ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI6415 good C KOG1261 Energy production and conversion Malate synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI6415 2233681 2232017 -555
(555 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNL117w [C] KOG1261 Malate synthase 909 0.0
YIR031c [C] KOG1261 Malate synthase 877 0.0
CE23521_2 [C] KOG1261 Malate synthase 472 e-133
CE23529_2 [C] KOG1261 Malate synthase 458 e-129
At5g03860 [C] KOG1261 Malate synthase 439 e-123
>YNL117w [C] KOG1261 Malate synthase
Length = 554
Score = 909 bits (2348), Expect = 0.0
Identities = 440/554 (79%), Positives = 487/554 (87%)
Query: 1 MVKKNLENVQLLVDVDTKPQFSPSTTTVADILTKDALEFVVLLHRTFNKTRLQLLENRQI 60
MVK +L+NV+LLVDVD +P F PS+TTV DILTKDALEF+VLLHRTFN R QLLENRQ+
Sbjct: 1 MVKVSLDNVKLLVDVDKEPFFKPSSTTVGDILTKDALEFIVLLHRTFNNKRKQLLENRQV 60
Query: 61 VQQKIDSGEYEFNFLKETEAIRNDPTWQGPPLAPGLIDRSTEITGPPLRNMLVNALNSDV 120
VQ+K+DSG Y +FL ET IRNDPTWQGP LAPGLI+RSTEITGPPLRNML+NALN+ V
Sbjct: 61 VQKKLDSGSYHLDFLPETANIRNDPTWQGPILAPGLINRSTEITGPPLRNMLINALNAPV 120
Query: 121 KTYMTDFEDSASPTWVNVIYGQVNLYDAIRDQIDFVTPRKEYKLRGKIADLPTLIVRPRG 180
TYMTDFEDSASPTW N++YGQVNLYDAIR+QIDF TPRK YKL G +A+LPT+IVRPRG
Sbjct: 121 NTYMTDFEDSASPTWNNMVYGQVNLYDAIRNQIDFDTPRKSYKLNGNVANLPTIIVRPRG 180
Query: 181 WHMVDNHILVDGEPISASILDFGLYFYHNAKKLIEIGKGPYFYLPKMEHHLEAKLWNDVF 240
WHMV+ H+ VD EPISASI DFGLYFYHNAK+LI++GKGPYFYLPKMEHHLEAKLWNDVF
Sbjct: 181 WHMVEKHLYVDDEPISASIFDFGLYFYHNAKELIKLGKGPYFYLPKMEHHLEAKLWNDVF 240
Query: 241 QVAQDSLAIPRGTIRATVLIETLPAAFQMEEIIWQLRQHSSGLNCGRWDYIFSTIKRLRN 300
VAQD + IPRGTIRATVLIETLPAAFQMEEII+QLRQHSSGLNCGRWDYIFSTIKRLRN
Sbjct: 241 CVAQDYIGIPRGTIRATVLIETLPAAFQMEEIIYQLRQHSSGLNCGRWDYIFSTIKRLRN 300
Query: 301 KPEHVLPNRDQVTMTSPFMNAYVKRLIKICHGRGVHAMGGMAAQIPIKDNEEANNAAMTK 360
P H+LPNR+QVTMTSPFM+AYVKRLI CH RGVHAMGGMAAQIPIKD+ AN AMTK
Sbjct: 301 DPNHILPNRNQVTMTSPFMDAYVKRLINTCHRRGVHAMGGMAAQIPIKDDPAANEKAMTK 360
Query: 361 VRNDKIRELTNGHDGSWVAHPALAAICNEVFAEMGTPNQIYFVPETSVAASDLLNTNIEG 420
VRNDKIRELTNGHDGSWVAHPALA ICNEVF MGTPNQIYF+PE V A++LL T I
Sbjct: 361 VRNDKIRELTNGHDGSWVAHPALAPICNEVFINMGTPNQIYFIPENVVTAANLLETKIPN 420
Query: 421 GEITTAGIKQNLDIGLQYMEAWLRGSGCVPINNLMEDAATAEVSRCQLHQWVKHKIVLKD 480
GEITT GI QNLDIGLQYMEAWLRGSGCVPINNLMEDAATAEVSRCQL+QWVKH + LKD
Sbjct: 421 GEITTEGIVQNLDIGLQYMEAWLRGSGCVPINNLMEDAATAEVSRCQLYQWVKHGVTLKD 480
Query: 481 TGKQVTPALAKEILYQQAKELAAKSPVGDANKFELAATYFEPEITGEKFSEFLTTLLYEE 540
TG++VTP L ++IL +Q + L+ SP+GD NKF LAA YF PEI GEKFSEFLTTLLY+E
Sbjct: 481 TGEKVTPELTEKILKEQVERLSKASPLGDKNKFALAAKYFLPEIRGEKFSEFLTTLLYDE 540
Query: 541 IITVNSTPYDLSKL 554
I++ +TP DLSKL
Sbjct: 541 IVSTKATPTDLSKL 554
>YIR031c [C] KOG1261 Malate synthase
Length = 554
Score = 877 bits (2267), Expect = 0.0
Identities = 422/554 (76%), Positives = 481/554 (86%)
Query: 1 MVKKNLENVQLLVDVDTKPQFSPSTTTVADILTKDALEFVVLLHRTFNKTRLQLLENRQI 60
MVK +L+N L D+DT PQF PS TTVADILTKDALEF+VLLHRTFN TR QLL NR
Sbjct: 1 MVKISLDNTALYADIDTTPQFEPSKTTVADILTKDALEFIVLLHRTFNSTRKQLLANRSN 60
Query: 61 VQQKIDSGEYEFNFLKETEAIRNDPTWQGPPLAPGLIDRSTEITGPPLRNMLVNALNSDV 120
+Q K+DSGEY F+FL ETE IRNDPTWQG APGLI+RS+EITGPPLRNMLVNALN++V
Sbjct: 61 LQSKLDSGEYRFDFLPETEQIRNDPTWQGAIPAPGLINRSSEITGPPLRNMLVNALNAEV 120
Query: 121 KTYMTDFEDSASPTWVNVIYGQVNLYDAIRDQIDFVTPRKEYKLRGKIADLPTLIVRPRG 180
TYMTDFEDS+SPTW N+IYGQVNLYDAIR+QIDF TPRKEY+L+ I+ LPTLIVRPRG
Sbjct: 121 TTYMTDFEDSSSPTWENMIYGQVNLYDAIRNQIDFKTPRKEYRLKDDISRLPTLIVRPRG 180
Query: 181 WHMVDNHILVDGEPISASILDFGLYFYHNAKKLIEIGKGPYFYLPKMEHHLEAKLWNDVF 240
WHMV+ H+ +D EPISASI DFGLYFYHNAK+L++IGKGPYFYLPKMEHH+E KLWND+F
Sbjct: 181 WHMVEKHLYIDDEPISASIFDFGLYFYHNAKELVKIGKGPYFYLPKMEHHMEVKLWNDIF 240
Query: 241 QVAQDSLAIPRGTIRATVLIETLPAAFQMEEIIWQLRQHSSGLNCGRWDYIFSTIKRLRN 300
VAQD + +PRGTIRATVLIETLPAAFQMEEII+Q+R+HSSGLNCGRWDYIFSTIK+LRN
Sbjct: 241 CVAQDFIGMPRGTIRATVLIETLPAAFQMEEIIYQIREHSSGLNCGRWDYIFSTIKKLRN 300
Query: 301 KPEHVLPNRDQVTMTSPFMNAYVKRLIKICHGRGVHAMGGMAAQIPIKDNEEANNAAMTK 360
PEHVLPNRD VTMTSPFM+AYVKRLI CH RGVHAMGGMAAQIPIKD+ +AN AAM K
Sbjct: 301 LPEHVLPNRDLVTMTSPFMDAYVKRLINTCHRRGVHAMGGMAAQIPIKDDPKANEAAMNK 360
Query: 361 VRNDKIRELTNGHDGSWVAHPALAAICNEVFAEMGTPNQIYFVPETSVAASDLLNTNIEG 420
VRNDKIRE+ NGHDGSWVAHPALA ICNEVF+ MGT NQIYFVP+ V +SDLLNT I+
Sbjct: 361 VRNDKIREMKNGHDGSWVAHPALAPICNEVFSNMGTANQIYFVPDVHVTSSDLLNTKIQD 420
Query: 421 GEITTAGIKQNLDIGLQYMEAWLRGSGCVPINNLMEDAATAEVSRCQLHQWVKHKIVLKD 480
++TT GI+ NLDIGLQYMEAWLRGSGCVPIN+LMEDAATAEVSRCQL+QWVKH +VL D
Sbjct: 421 AQVTTEGIRVNLDIGLQYMEAWLRGSGCVPINHLMEDAATAEVSRCQLYQWVKHGVVLSD 480
Query: 481 TGKQVTPALAKEILYQQAKELAAKSPVGDANKFELAATYFEPEITGEKFSEFLTTLLYEE 540
TG +VTP L +IL ++ +LA+ SP+G+ NKF LAA YF PE+TG+ FS+FLTTLLY+E
Sbjct: 481 TGDKVTPELTAKILNEETAKLASASPLGEKNKFALAAKYFLPEVTGKIFSDFLTTLLYDE 540
Query: 541 IITVNSTPYDLSKL 554
II ++ P DLSKL
Sbjct: 541 IIKPSAKPVDLSKL 554
>CE23521_2 [C] KOG1261 Malate synthase
Length = 530
Score = 472 bits (1214), Expect = e-133
Identities = 251/517 (48%), Positives = 334/517 (64%), Gaps = 11/517 (2%)
Query: 31 ILTKDALEFVVLLHRTFNKTRLQLLENRQIVQQKIDSGEYEFNFLKETEAIRNDPTWQGP 90
ILT DAL F+ L+ FN RL+LL R VQ I++ + +F KETE +R+D W+G
Sbjct: 18 ILTPDALRFLHDLNTEFNPRRLRLLSKRNQVQADINNSLWFPDFNKETEVLRSDQGWKGA 77
Query: 91 PLAPGLIDRSTEITGPPLRNMLVNALNSDVKTYMTDFEDSASPTWVNVIYGQVNLYDAIR 150
+ L DR EITGP R M++NA+NS +M DFEDS SPTW N + GQ+NLYDA+R
Sbjct: 78 EIPRDLQDRRVEITGPTDRKMVINAMNSGANVFMADFEDSNSPTWRNQLEGQINLYDAVR 137
Query: 151 DQIDFVTP--RKEYKLRGKIADLPTLIVRPRGWHMVDNHILVDGEPISASILDFGLYFYH 208
+ I + P +KEY L K A L VRPRGWH+ + H+L+ +P S S+ DFGL+ +H
Sbjct: 138 NNISYTHPTTKKEYTLNEKHA---VLKVRPRGWHLPEKHVLIHNQPTSGSLFDFGLFVFH 194
Query: 209 NAKKLIEIGKGPYFYLPKMEHHLEAKLWNDVFQVAQDSLAIPRGTIRATVLIETLPAAFQ 268
NAK LI G GPYFYLPK++ EA+LW DVF+ +D L + RGTI+ TVLIE L A+FQ
Sbjct: 195 NAKALIAQGSGPYFYLPKLQSAEEAQLWADVFKYTEDKLGLARGTIKCTVLIEHLLASFQ 254
Query: 269 MEEIIWQLRQHSSGLNCGRWDYIFSTIKRLRNKPEHVLPNRDQVTMTSPFMNAYVKRLIK 328
+ EII L+ + GLNCGRWDYIFS IK +N + +LP+R Q+ MT+PFM Y +IK
Sbjct: 255 LHEIIHALKDNIVGLNCGRWDYIFSYIKTFQNHRKFLLPDRFQIGMTAPFMRNYSLEVIK 314
Query: 329 ICHGRGVHAMGGMAAQIPIKDNEEANNAAMTKVRNDKIRELTNGHDGSWVAHPALAAICN 388
CH RG+HAMGGMAAQIPIK ++ AN+ A VR DK RE T+GHDG+WVAHP L +
Sbjct: 315 ACHLRGIHAMGGMAAQIPIKHDQVANDKAFALVRADKEREATDGHDGTWVAHPGLVPLAK 374
Query: 389 EVFAE-MGTPNQI-YFVPETSVAASDLLNTNIEGGEITTAGIKQNLDIGLQYMEAWLRGS 446
VF + M PNQI + + DL T I G T AG + N+ + L Y+++WLRG+
Sbjct: 375 RVFDQMMPKPNQISKNLTRANCTKEDL--TVIPEGTRTEAGFRHNISVTLGYLDSWLRGT 432
Query: 447 GCVPINNLMEDAATAEVSRCQLHQWVKHKIVLKDTGKQVTPALAKEILYQQAKELAAKSP 506
GCVP+ NLMEDAATAE+SR QL QW+ H L+D G+ + L K+ + + + ++
Sbjct: 433 GCVPLYNLMEDAATAEISRAQLWQWLHHDAKLED-GRTIDAGLVKQTIAAETERRLIRAG 491
Query: 507 VGDANKFELAATYFEPEITGEKFSEFLTTLLYEEIIT 543
N+ AA E +T EK S+FLTT Y+ +++
Sbjct: 492 -SVVNRIPEAADLLEKFVTEEKMSDFLTTDAYDRLVS 527
>CE23529_2 [C] KOG1261 Malate synthase
Length = 516
Score = 458 bits (1179), Expect = e-129
Identities = 241/516 (46%), Positives = 330/516 (63%), Gaps = 23/516 (4%)
Query: 31 ILTKDALEFVVLLHRTFNKTRLQLLENRQIVQQKIDSGEYEFNFLKETEAIRNDPTWQGP 90
ILT DAL F+ L+ FN RL+LL R VQ+ I++ + +F KET+ +R+D W+G
Sbjct: 18 ILTPDALRFLHDLNIEFNPRRLKLLSKRNKVQEDINNSIWFPDFNKETQVLRDDQGWKGS 77
Query: 91 PLAPGLIDRSTEITGPPLRNMLVNALNSDVKTYMTDFEDSASPTWVNVIYGQVNLYDAIR 150
+ L DR EITGPP R ++NALNS +MTDFEDS SPTW N I GQVN+YDA+R
Sbjct: 78 QIPDDLKDRRVEITGPPDRKSVINALNSGANVFMTDFEDSNSPTWRNQIEGQVNMYDAVR 137
Query: 151 DQIDFVTP--RKEYKLRGKIADLPTLIVRPRGWHMVDNHILVDGEPISASILDFGLYFYH 208
+ I + P +KEYKL K A L+VRPRGWH+ + H+ + +P S ++ DFGL+ +H
Sbjct: 138 NNISYTHPTTKKEYKLDEKHA---VLMVRPRGWHLTEKHVQIHNQPTSGTLFDFGLFVFH 194
Query: 209 NAKKLIEIGKGPYFYLPKMEHHLEAKLWNDVFQVAQDSLAIPRGTIRATVLIETLPAAFQ 268
NAK LI G GPYFYLPK+++ EA+LW+DVF+ +D L + RGTI+ TVLIE L A FQ
Sbjct: 195 NAKALISKGSGPYFYLPKLQNAEEAQLWSDVFKFTEDKLGLSRGTIKCTVLIEHLLATFQ 254
Query: 269 MEEIIWQLRQHSSGLNCGRWDYIFSTIKRLRNKPEHVLPNRDQVTMTSPFMNAYVKRLIK 328
+ EII L+ H GLNCGRWD+ S +R Q++MT+PFM Y LIK
Sbjct: 255 LHEIIHSLKDHVVGLNCGRWDFFCS--------------HRHQISMTAPFMRNYSLELIK 300
Query: 329 ICHGRGVHAMGGMAAQIPIKDNEEANNAAMTKVRNDKIRELTNGHDGSWVAHPALAAICN 388
CH RG+HAMGGMAA IPIK ++ AN+ A V +DK RE+T+GHDG+W+AHP L +
Sbjct: 301 TCHQRGIHAMGGMAAHIPIKHDQIANDKAFALVTSDKQREVTDGHDGTWIAHPGLVPVAQ 360
Query: 389 EVFAE-MGTPNQIYFVPETSVAASDLLNTNIEGGEITTAGIKQNLDIGLQYMEAWLRGSG 447
VF E M PNQI + +A + L T + G T G + N+ + L Y++ WLRG G
Sbjct: 361 RVFNEMMPRPNQIDKLLNRPIATKEQL-TMVPEGTRTDLGFRNNISVTLGYLDYWLRGVG 419
Query: 448 CVPINNLMEDAATAEVSRCQLHQWVKHKIVLKDTGKQVTPALAKEILYQQAKELAAKSPV 507
CVP++NLMED ATAE+SR QL QW+ H+ L+D G+ + L K+ + +A+ + ++
Sbjct: 420 CVPLDNLMEDLATAEISRAQLWQWLHHEAKLED-GRVIDTGLVKQTIAAEAEHRSIRAG- 477
Query: 508 GDANKFELAATYFEPEITGEKFSEFLTTLLYEEIIT 543
N+ AA E +T EK S+FLT Y+++++
Sbjct: 478 SVVNRIPEAAELLEKLVTEEKLSDFLTLDAYDKLVS 513
>At5g03860 [C] KOG1261 Malate synthase
Length = 562
Score = 439 bits (1130), Expect = e-123
Identities = 252/542 (46%), Positives = 332/542 (60%), Gaps = 27/542 (4%)
Query: 29 ADILTKDALEFVVLLHRTFNKTRLQLLENRQIVQQKIDSGEYEFNFLKETEAIRNDPTWQ 88
A ILT++AL FV L R F +E R+ +++ +SG F T+ IR D W
Sbjct: 32 AKILTREALSFVAELQREFRGHVKYAMECRREARRRYNSGAVP-GFDPSTKFIR-DGDWS 89
Query: 89 GPPLAPGLIDRSTEITGPPLRNMLVNALNSDVKTYMTDFEDSASPTWVNVIYGQVNLYDA 148
+ P + DR EITGP R M++NALNS K +M DFED+ SP+W N++ G VNL DA
Sbjct: 90 CASVPPAVADRRVEITGPVERKMIINALNSGAKVFMADFEDALSPSWENLMRGHVNLKDA 149
Query: 149 IRDQIDF--VTPRKEYKLRGKIADLPTLIVRPRGWHMVDNHILVDGEPISASILDFGLYF 206
+ I F + + YKL + A L VRPRGWH+ + HIL+DGEP + ++DFGLYF
Sbjct: 150 VDGSITFHDKSRNRVYKLNDQTAKL---FVRPRGWHLPEAHILIDGEPATGCLVDFGLYF 206
Query: 207 YHNAKKLIEI---GKGPYFYLPKMEHHLEAKLWNDVFQVAQDSLAIPRGTIRATVLIETL 263
+HN K + G GP+FYLPKMEH EAK+WN VF+ A+ I RG+IRATVLIETL
Sbjct: 207 FHNYAKFRQTQGSGFGPFFYLPKMEHSREAKIWNSVFERAEKMAGIERGSIRATVLIETL 266
Query: 264 PAAFQMEEIIWQLRQHSSGLNCGRWDYIFSTIKRLRNKPEHVLPNRDQVTMTSPFMNAYV 323
PA FQM EI+++LR HS GLNCGRWDYIFS +K + P+ +LP+R V M FM +Y
Sbjct: 267 PAVFQMNEILYELRDHSVGLNCGRWDYIFSYVKTFQAHPDRLLPDRVLVGMGQHFMRSYS 326
Query: 324 KRLIKICHGRGVHAMGGMAAQIPIKDNEEANNAAMTKVRNDKIRELTNGHDGSWVAHPAL 383
LI+ CH RGVHAMGGMAAQIPI+D+ +AN A+ VR DK+RE+ GHDG+W AHP L
Sbjct: 327 DLLIRTCHKRGVHAMGGMAAQIPIRDDPKANEMALDLVRKDKLREVRAGHDGTWAAHPGL 386
Query: 384 AAICNEVF-AEMG-TPNQIYFVPETSVAA---SDLLNTNIEGGEITTAGIKQNLDIGLQY 438
IC E F MG +PNQI V AA DLL I G T G++ N +G+QY
Sbjct: 387 IPICMEAFTGHMGKSPNQIKSVKREDAAAITEEDLL--QIPRGVRTLEGLRLNTRVGIQY 444
Query: 439 MEAWLRGSGCVPINNLMEDAATAEVSRCQLHQWVKHKIVLKDTGKQVTPALAKEI---LY 495
+ AWL GSG VP+ NLMEDAATAE+SR Q QW+++ + L G V ++KE+ +
Sbjct: 445 LAAWLTGSGSVPLYNLMEDAATAEISRVQNWQWIRYGVELDGDGLGV--RVSKELFGRVV 502
Query: 496 QQAKELAAKSPVGDANK---FELAATYFEPEITGEKFSEFLTTLLYEEIITVNSTPYDLS 552
++ E K D K ++ A F + T + +FLT +Y I+ P ++S
Sbjct: 503 EEEMERIEKEVGKDKFKNGMYKEACKMFTKQCTAPELDDFLTLAVYNHIVA--HYPINVS 560
Query: 553 KL 554
+L
Sbjct: 561 RL 562
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,952,890
Number of Sequences: 60738
Number of extensions: 1484965
Number of successful extensions: 3281
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3266
Number of HSP's gapped (non-prelim): 5
length of query: 555
length of database: 30,389,216
effective HSP length: 111
effective length of query: 444
effective length of database: 23,647,298
effective search space: 10499400312
effective search space used: 10499400312
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)