ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI6509 good TR KOG2283 General function prediction only Clathrin coat dissociation kinase GAK/PTEN/Auxilin and related tyrosine phosphatases
r_klactVI6509 good TR KOG2283 Signal transduction mechanisms Clathrin coat dissociation kinase GAK/PTEN/Auxilin and related tyrosine phosphatases
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI6509 2267020 2265722 -433
(433 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNL128w [TR] KOG2283 Clathrin coat dissociation kinase GAK/PTEN/... 261 1e-69
SPBC609.02 [TR] KOG2283 Clathrin coat dissociation kinase GAK/PT... 68 2e-11
7297635 [TR] KOG2283 Clathrin coat dissociation kinase GAK/PTEN/... 57 4e-08
Hs4506249 [TR] KOG2283 Clathrin coat dissociation kinase GAK/PTE... 57 5e-08
CE26385 [TR] KOG2283 Clathrin coat dissociation kinase GAK/PTEN/... 51 4e-06
>YNL128w [TR] KOG2283 Clathrin coat dissociation kinase GAK/PTEN/Auxilin and
related tyrosine phosphatases
Length = 434
Score = 261 bits (668), Expect = 1e-69
Identities = 157/447 (35%), Positives = 244/447 (54%), Gaps = 46/447 (10%)
Query: 5 KFNPEHIIKSIYSSPFNQYKNNMGLVLDVSYVTDNIIVCSYPVMKYPKMFYRNSLVDLVT 64
K+ P +++K I S P + KN++GL LD+SY+ N+IVCSYPV YPK+ YRNSL DL+
Sbjct: 16 KWKPVNLMKKILSLPMKKTKNDIGLRLDISYILVNLIVCSYPVNTYPKLLYRNSLDDLIL 75
Query: 65 YLDANHGRKNWKIYNLKAEMNDSDYTDDD---FAMVLESKHVTPPAGKSEKITSTFRSRK 121
+L HG+ N++I+N + E DSDY D+D A ESK RS
Sbjct: 76 FLTVYHGKGNFRIFNFRGEKEDSDYKDNDLIGIAAKFESKDFE---------IQELRSTL 126
Query: 122 MLEDHCNVSGIKLLFRSNADEIVKPPISVKSHLLRAGWIDHSPPSFLHLQNLIDDIRD-- 179
+ + +S I L R+ +E I R GW+DH PP F L+ ++D I +
Sbjct: 127 INDGKIPISPIDLETRTLVEEETNNVICE-----RIGWLDHFPPPFELLEEIVDGIENYL 181
Query: 180 TVSRGKVAVIHCKMGKGRSGTLVVAYLMTYLQLPRHEAQSLFLSTRFKTGISKGVTIASQ 239
+VS+ +VAV+HC+MGKGRSG + VAYLM YLQ P EA+ +F+ RFK G++ GVTI SQ
Sbjct: 182 SVSKNRVAVLHCRMGKGRSGMITVAYLMKYLQCPLGEARLIFMQARFKYGMTNGVTIPSQ 241
Query: 240 LRYLKYQEMFLRYEAKQREEIIKNLSTIEFK--ILSIEFTNSIGQYSSEFLKKANNTTVS 297
LRYL+Y E F+ +E +E I +FK + + F +SE + V+
Sbjct: 242 LRYLRYHEFFITHEKAAQEGISNEAVKFKFKFRLAKMTFLRPSSLITSE------SAIVT 295
Query: 298 IKIQSHSSGKSGLI-------DLFSKQCSTGKGSLRSLTSKQIIVPNINVTFSDIRLSFG 350
KIQ ++ ++ L+ D+ + +C G++ + + + +D R+ F
Sbjct: 296 TKIQHYNDDRNALLTRKVVYSDIMAHECG---GNMTFIFGRDYLTLE-----NDCRIEFT 347
Query: 351 IRSKTSQFINNVSHLSSFSSCWLNLYWESVLQSKNLDNDSNYI----LPKNERFEFYIPW 406
+ + S+ +++ +S +SCWLN+Y E+++ D+ +Y L ++E I W
Sbjct: 348 LGTSKSKAASSIISWTSCASCWLNIYLETLMHIIKDDSSPDYFQVERLKRDEMLGTTISW 407
Query: 407 EELDGFKGTSGKGLKLFDSISIRWMLL 433
+ELDGF S GLKLF ++ + W ++
Sbjct: 408 QELDGFGELSTHGLKLFQALKLEWEII 434
>SPBC609.02 [TR] KOG2283 Clathrin coat dissociation kinase GAK/PTEN/Auxilin and
related tyrosine phosphatases
Length = 348
Score = 68.2 bits (165), Expect = 2e-11
Identities = 67/284 (23%), Positives = 123/284 (42%), Gaps = 32/284 (11%)
Query: 134 LLFRSNADEIVKPPISVKSHLLRAGWIDHSPPSFLHLQNLIDDIRDTVSRGK---VAVIH 190
+L A+E V K +++ G+ DH+PP L L ++ ++ D + + + V+H
Sbjct: 70 ILLNLCAEETVYHLELFKPNVINYGFQDHNPPPLLFLWAIVMNM-DALFQTQPLLTLVVH 128
Query: 191 CKMGKGRSGTLVVAYLMTYLQLPRHEAQSLFLSTRFKTGISKGVTIASQLRYLKYQEMFL 250
CK GKGR+GT++ +YL+ + L ++ L+ R G G+TI+SQ+RY+ Y E+
Sbjct: 129 CKAGKGRTGTVICSYLVAFGGLTAKQSLELYTEKRMVRG--HGLTISSQIRYVYYIEILK 186
Query: 251 RYEAKQREEIIKNLSTIEFKILSIEFTNSIGQYSSEFLKKANNTTVSIKIQSHSSGKSGL 310
++ L +EF T + S + L N+++ + + + S G++
Sbjct: 187 QFP--------NYLKAVEFN------TGTTFFKSFKCLNIKKNSSLILSLHAFSKGRNIN 232
Query: 311 IDLFSKQCSTGKGSLRSLTSKQIIVPNINVTFSDIRLSFGIRSKTSQFINNVSHLSSFSS 370
K ++ K+I N+ S+ L + K QF + S
Sbjct: 233 PVALWKSSDISSHNVSIKEGKRIWGIQCNLETSEKDLLLRVERK-GQF-----YFPSSVQ 286
Query: 371 CWLNLYWESVL----QSKNLDNDSNYILPKNERFEFYIPWEELD 410
CW + +++ +L N N L + F W E+D
Sbjct: 287 CWFHTHFQPMLVEYTNGINFQQGINSFLQGQQSISF--SWSEMD 328
>7297635 [TR] KOG2283 Clathrin coat dissociation kinase GAK/PTEN/Auxilin and
related tyrosine phosphatases
Length = 514
Score = 57.4 bits (137), Expect = 4e-08
Identities = 31/87 (35%), Positives = 49/87 (55%), Gaps = 3/87 (3%)
Query: 161 DHSPPSFLHLQNLIDDIRDTVSR--GKVAVIHCKMGKGRSGTLVVAYLMTYLQLPRHEAQ 218
DH+PP+ +Q D+ + V +HCK GKGR+GT++ AYL+ + + + +
Sbjct: 100 DHNPPTIELIQRFCSDVDMWLKEDSSNVVAVHCKAGKGRTGTMICAYLV-FSGIKKSADE 158
Query: 219 SLFLSTRFKTGISKGVTIASQLRYLKY 245
+L +T KGVTI SQ RY++Y
Sbjct: 159 ALAWYDEKRTKDRKGVTIPSQRRYVQY 185
Score = 49.7 bits (117), Expect = 9e-06
Identities = 27/74 (36%), Positives = 44/74 (58%), Gaps = 2/74 (2%)
Query: 10 HIIKSIYSSPFNQYKNNMGLVLDVSYVTDNIIVCSYPVMKYPKMFYRNSLVDLVTYLDAN 69
++I+++ S +YK G LD++Y+ DNII YP + +RN L D+ L+ N
Sbjct: 10 NVIRNVVSKKRIRYKEK-GYDLDLTYINDNIIAMGYPAPDKLEGLFRNRLEDVFKLLEEN 68
Query: 70 HGRKNWKIYNLKAE 83
H +++KIYNL +E
Sbjct: 69 HA-QHYKIYNLCSE 81
>Hs4506249 [TR] KOG2283 Clathrin coat dissociation kinase GAK/PTEN/Auxilin and
related tyrosine phosphatases
Length = 403
Score = 57.0 bits (136), Expect = 5e-08
Identities = 33/93 (35%), Positives = 51/93 (54%), Gaps = 3/93 (3%)
Query: 161 DHSPPSFLHLQNLIDDIRDTVSR--GKVAVIHCKMGKGRSGTLVVAYLMTYLQLPRHEAQ 218
DH+PP ++ +D+ +S VA IHCK GKGR+G ++ AYL+ + + + +
Sbjct: 92 DHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQ-E 150
Query: 219 SLFLSTRFKTGISKGVTIASQLRYLKYQEMFLR 251
+L +T KGVTI SQ RY+ Y L+
Sbjct: 151 ALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLK 183
Score = 49.7 bits (117), Expect = 9e-06
Identities = 29/73 (39%), Positives = 46/73 (62%), Gaps = 3/73 (4%)
Query: 11 IIKSIYSSPFNQYKNNMGLVLDVSYVTDNIIVCSYPVMKYPKMFYRNSLVDLVTYLDANH 70
IIK I S +Y+ + G LD++Y+ NII +P + + YRN++ D+V +LD+ H
Sbjct: 4 IIKEIVSRNKRRYQED-GFDLDLTYIYPNIIAMGFPAERLEGV-YRNNIDDVVRFLDSKH 61
Query: 71 GRKNWKIYNLKAE 83
+ ++KIYNL AE
Sbjct: 62 -KNHYKIYNLCAE 73
>CE26385 [TR] KOG2283 Clathrin coat dissociation kinase GAK/PTEN/Auxilin and
related tyrosine phosphatases
Length = 962
Score = 50.8 bits (120), Expect = 4e-06
Identities = 50/199 (25%), Positives = 88/199 (44%), Gaps = 21/199 (10%)
Query: 55 YRNSLVDLV-----TYLDANHGRKNWKIYNLKAEMNDSDY-TDDDFAMVLESKHVTPPAG 108
Y NS+V+ + T + +N R ++ +L D Y TD A+ + +
Sbjct: 37 YHNSIVERIRHIFRTAVSSNRCRTEYQNIDL-----DCAYITDRIIAIGYPATGIEANFR 91
Query: 109 KSEKITSTFRSRKMLEDHCNVSGIKLLFRSNADEIVKPPISVKSHLLRAGWIDHSPPSFL 168
S+ T F +R+ + + V ++ + +AD +++ DH PPS
Sbjct: 92 NSKVQTQQFLTRRHGKGNVKVFNLRGGYYYDADNF-------DGNVICFDMTDHHPPSLE 144
Query: 169 HLQNLIDDIRDTVSRGK--VAVIHCKMGKGRSGTLVVAYLMTYLQLPRHEAQSLFLSTRF 226
+ + ++ + V +HCK GKGR+G ++ A L+ Y+ Q L +
Sbjct: 145 LMAPFCREAKEWLEADDKHVIAVHCKAGKGRTGVMICA-LLIYINFYPSPRQILDYYSII 203
Query: 227 KTGISKGVTIASQLRYLKY 245
+T +KGVTI SQ RY+ Y
Sbjct: 204 RTKNNKGVTIPSQRRYIYY 222
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.135 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,834,156
Number of Sequences: 60738
Number of extensions: 1098242
Number of successful extensions: 3248
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 3232
Number of HSP's gapped (non-prelim): 9
length of query: 433
length of database: 30,389,216
effective HSP length: 109
effective length of query: 324
effective length of database: 23,768,774
effective search space: 7701082776
effective search space used: 7701082776
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)