ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI6588 good A KOG2636 RNA processing and modification Splicing factor 3a, subunit 3
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI6588 2294399 2292810 -530
(530 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL030w [A] KOG2636 Splicing factor 3a subunit 3 296 5e-80
SPBC36.09 [A] KOG2636 Splicing factor 3a subunit 3 147 5e-35
Hs5803167 [A] KOG2636 Splicing factor 3a subunit 3 129 1e-29
CE23981 [A] KOG2636 Splicing factor 3a subunit 3 122 1e-27
At5g06160 [A] KOG2636 Splicing factor 3a subunit 3 118 3e-26
7296720 [A] KOG2636 Splicing factor 3a subunit 3 115 2e-25
ECU07g1140 [A] KOG2636 Splicing factor 3a subunit 3 67 9e-11
>YDL030w [A] KOG2636 Splicing factor 3a subunit 3
Length = 530
Score = 296 bits (758), Expect = 5e-80
Identities = 183/529 (34%), Positives = 268/529 (50%), Gaps = 11/529 (2%)
Query: 10 EERRKILEELEVIEDAISSRLRRNPEIFYRFNEALHNLGVEAWAVPKLDVSANRIYXXXX 69
E RR +LEE+E+IE+AI+ R++RNPE++Y + + + + ++ N+IY
Sbjct: 5 ETRRSLLEEMEIIENAIAERIQRNPELYYHYIQESSKVFPDTKLPRSSLIAENKIYKFKK 64
Query: 70 XXXXXXQIKAQEYEIALFLGRYRRLLKSLQKHKLSEEHLEYFRDTNASFEQFETVTTEVL 129
QI Q++EI +FL Y+ ++ K EE E +D + + + E+
Sbjct: 65 VKRKRKQIILQQHEINIFLRDYQEKQQTFNKINRPEETQEDDKDLPNFERKLQQLEKELK 124
Query: 130 EGQVNSRSLVEVKSD-YEMFSGPQAASK--NIISPIGQIIDLSRNFARDENFGTYLDLSE 186
N + K D Y +FS S+ NI+S + +DL+ F RDE +G Y++L +
Sbjct: 125 NEDENFELDINSKKDKYALFSSSSDPSRRTNILSDRARDLDLNEIFTRDEQYGEYMELEQ 184
Query: 187 QFKKWLNIIKRADLTILSFLKMLESF-ETYDYLLNPSLDRDTKEYNQLVTELLXXXXXXX 245
WLN+IKR D ++L FL +LE F + YLL P +DR Y + +L
Sbjct: 185 FHSLWLNVIKRGDCSLLQFLDILELFLDDEKYLLTPPMDRKNDRYMAFLLKLSKYVETFF 244
Query: 246 XXXXPLVDKEILSKTIHNGFDKYVRTGITESPSKESGKMYSVVFNRWFDSQSEFNTHVQT 305
L+D + I + F+ G S +K +Y +RWF + S F +H+
Sbjct: 245 FKSYALLDAAAVENLIKSDFEHSYCRGSLRSEAKG---IYCPFCSRWFKTSSVFESHLVG 301
Query: 306 EQFNIALSRNRKRLMKEYTFHCFSKFLSKVLQNTISFTERKLAXXXXXXXXXXXXXXXXY 365
+ S+ R + EY H + K+L+ T SF ERKLA Y
Sbjct: 302 KIHKKNESKRRNFVYSEYKLHRYLKYLNDEFSRTRSFVERKLAFTANERMAEMDILTQKY 361
Query: 366 ESPIYAKDESEDKPSYIDNAEDTAQKESMDPTNPYNLPLGPDGFPIPRWLIKVQGLDVKY 425
E+P Y E E D +E +++PLGPDG P+P WL K+ GLD +Y
Sbjct: 362 EAPAYDSTEKEGAEQVDGEQRDGQLQEEHLSGKSFDMPLGPDGLPMPYWLYKLHGLDREY 421
Query: 426 VCEICGNQIYHGRREFEKHFPLKKHSEGLKSLGITPSLAFKDVTTIVEVTELWKALQAKS 485
CEIC N++Y+GRR FE+HF ++H L+ LGI PS FK +T I E ELWK +Q +S
Sbjct: 422 RCEICSNKVYNGRRTFERHFNEERHIYHLRCLGIEPSSVFKGITKIKEAQELWKNMQGQS 481
Query: 486 TLKS---GEPFSKISTVKEP-EMVLETEDNEGNVMSVQVYEELKKQGLL 530
L S P S +K P E+ LE ED EGNVMS +VY+ELKKQGL+
Sbjct: 482 QLTSIAAVPPKPNPSQLKVPTELELEEEDEEGNVMSKKVYDELKKQGLV 530
>SPBC36.09 [A] KOG2636 Splicing factor 3a subunit 3
Length = 492
Score = 147 bits (370), Expect = 5e-35
Identities = 125/481 (25%), Positives = 210/481 (42%), Gaps = 60/481 (12%)
Query: 81 EYEIALFLGRYRRLLKSLQKHKLSEEHLEYFRDTNASFEQFETVTTEVLEGQVNSRSLVE 140
E++ A FL ++R K L +S E + +D + + TE + + +
Sbjct: 41 EHQSAQFLNQFRETSKKLL---VSHESSDRLKDQEVARINADDDLTEFYKSLGEIQEFHK 97
Query: 141 VKSDYEMFSGPQAASKNIISPIGQIIDLSRNFARDENFGTYLDLSEQFKKWLNIIKRADL 200
D+++ Q S P I ++ F +E +G ++DL+E +++++N+ +++
Sbjct: 98 KYPDHKVEDLSQLYSIKPSQP--GIDEIDTLFRGEEMYGRFMDLNECYEEYINL---SNV 152
Query: 201 TILSFLKMLESFETYDYLLNPSLDRDTKEYNQLVTELLXXXXXXXXXXXPLVDKEILSKT 260
+S+L+ L++ E +D + P ++ Y +T L PL + + +
Sbjct: 153 QHISYLEYLKNLEDFDQIPKPEKNQT---YINYITHLYEYLVSFYRRTHPLSNLDKIIAV 209
Query: 261 IHNGFDKYVRTGI---------TESPSKESGK-MYSVVFNRWFDSQSEFNTHVQTEQFNI 310
FD G+ E K+S + Y V ++F + F H +++ N
Sbjct: 210 FDTEFDAAWEAGLPGWYSHNAEAEKDGKDSTEAFYCEVCQKFFGKITVFEAHKKSKAHNK 269
Query: 311 ALSR----------NRKRLMK--------EYTFHCFSKFLSKVLQNTISFTERK---LAX 349
A+ R N K E+ + L V ++T R+ A
Sbjct: 270 AVKRMQSSSPSTTSNTNEKQKGPKAIARIEFLIKKLTSLLDDVRKDTRENVVRRQTLTAA 329
Query: 350 XXXXXXXXXXXXXXXYESPIYAKDESEDKPSYIDNAEDTAQKESMDPTNPYNLPLGPDGF 409
+P + + ++D+ S D+ E NP LPLG DG
Sbjct: 330 ERLAEVEAAEREAFEQSTPSVSVEGNQDEESDQDDEEKIY--------NPLKLPLGWDGK 381
Query: 410 PIPRWLIKVQGLDVKYVCEICGNQIYHGRREFEKHFPLKKHSEGLKSLGITPSLAFKDVT 469
PIP WL K+ GL ++ CEICGN +Y GR+ F+KHF ++H GLK LGI+PS F +T
Sbjct: 382 PIPFWLWKLHGLGKEFPCEICGNYVYMGRKAFDKHFTEQRHIYGLKCLGISPSPLFNQIT 441
Query: 470 TIVEVTELWKALQAKSTLKSGEPFSKISTVKEPEMVLETEDNEGNVMSVQVYEELKKQGL 529
+I E +LW+ + S + S + E ED+EGNVMS +VY +LK QGL
Sbjct: 442 SIDEALQLWQKYKVDSKKRETTMAS----------LNEMEDDEGNVMSEKVYNDLKAQGL 491
Query: 530 L 530
L
Sbjct: 492 L 492
>Hs5803167 [A] KOG2636 Splicing factor 3a subunit 3
Length = 501
Score = 129 bits (323), Expect = 1e-29
Identities = 103/390 (26%), Positives = 162/390 (41%), Gaps = 49/390 (12%)
Query: 172 FARDENFGTYLDLSEQFKKWLNIIKRADLTILSFLKMLESFETYDYLLNPSLDRDTKEYN 231
F +E +G YLDL + + K++N+ L +++L + +D L + +R EY
Sbjct: 130 FTDEEGYGRYLDLHDCYLKYINLKASEKLDYITYLSI------FDQLFDIPKERKNAEYK 183
Query: 232 QLVTELLXXXXXXXXXXXPLVDKEILSKTIHNGFDKYVRTGITESPSKESGKMYS----- 286
+ + LL PL D+ L I F+K G KE+ +
Sbjct: 184 RYLEMLLEYLQDYTDRVKPLQDQNELFGKIQAEFEKKWENGTFPGWPKETSSALTHAGAH 243
Query: 287 ---VVFNRWFDSQSEFNTHVQTEQFNIALSRNRKRLMKEYTFHCFSKFLSKVLQNTISFT 343
F+ W + S +++ +AL ++E FS + S
Sbjct: 244 LDLSAFSSWEELASLGLDRLKSAL--LALGLKCGGTLEERAQRLFSTKGKSLESLDTSLF 301
Query: 344 ERKLAXXXXXXXXXXXXXXXXYESPIYAKDESEDKPSYI------------------DNA 385
+ E+ IY E + ++ +
Sbjct: 302 AKNPKSKGTKRDTERNKDIAFLEAQIYEYVEILGEQRHLTHENVQRKQARTGEEREEEEE 361
Query: 386 EDTAQKESMDPTN-----PYNLPLGPDGFPIPRWLIKVQGLDVKYVCEICGNQIYHGRRE 440
E ++ ES D N P NLPLG DG PIP WL K+ GL++ Y CEICGN Y G +
Sbjct: 362 EQISESESEDEENEIIYNPKNLPLGWDGKPIPYWLYKLHGLNINYNCEICGNYTYRGPKA 421
Query: 441 FEKHFPLKKHSEGLKSLGITPSLAFKDVTTIVEVTELWKALQAKSTLKSGEPFSKISTVK 500
F++HF +H+ G++ LGI + F +VT I + LW L+ K S
Sbjct: 422 FQRHFAEWRHAHGMRCLGIPNTAHFANVTQIEDAVSLWAKLK----------LQKASERW 471
Query: 501 EPEMVLETEDNEGNVMSVQVYEELKKQGLL 530
+P+ E ED+ GNV++ + YE+LK+QGLL
Sbjct: 472 QPDTEEEYEDSSGNVVNKKTYEDLKRQGLL 501
>CE23981 [A] KOG2636 Splicing factor 3a subunit 3
Length = 500
Score = 122 bits (307), Expect = 1e-27
Identities = 63/158 (39%), Positives = 94/158 (58%), Gaps = 13/158 (8%)
Query: 373 DESEDKPSYIDNAEDTAQKESMDPTNPYNLPLGPDGFPIPRWLIKVQGLDVKYVCEICGN 432
+E E++ I+ E+ + P NP NLPLG DG PIP WL K+ GL++ Y CEICGN
Sbjct: 356 EEEEEEEIVIEEEEEIDESA---PYNPKNLPLGWDGKPIPYWLYKLHGLNLSYSCEICGN 412
Query: 433 QIYHGRREFEKHFPLKKHSEGLKSLGITPSLAFKDVTTIVEVTELWKALQAKSTLKSGEP 492
Q Y G + F+KHF +HS G++ LGI + F ++T I + +LW L+ + +
Sbjct: 413 QTYKGPKAFQKHFNEWRHSHGMRCLGIPNTSHFANITKIKDALDLWNKLKTEKEMAKW-- 470
Query: 493 FSKISTVKEPEMVLETEDNEGNVMSVQVYEELKKQGLL 530
P++ E ED+ GNV++ ++YE+LK+QGLL
Sbjct: 471 --------NPDIDEEYEDSSGNVVTRKMYEDLKRQGLL 500
>At5g06160 [A] KOG2636 Splicing factor 3a subunit 3
Length = 504
Score = 118 bits (295), Expect = 3e-26
Identities = 106/416 (25%), Positives = 170/416 (40%), Gaps = 62/416 (14%)
Query: 161 PIGQIIDLSRN------------FARDENFGTYLDLSEQFKKWLNIIKRADLTILSFLKM 208
P G+++D + + F+ +E G YLDL + + +++N + ++L +
Sbjct: 105 PSGRLVDANEDYEARLKEEPIIAFSGEEGNGRYLDLHDMYNQYINSKFGERVEYSAYLDV 164
Query: 209 LESFETYDYLLNPSLDRDTKEYNQLVTELLXXXXXXXXXXXPLVDKEILSKTIHNGFDKY 268
E L S ++Y + + LL PL D + + + + F++
Sbjct: 165 FSQPEKIPRKLKLS-----RQYMKYMEALLEYLVYFFQRTEPLQDLDRILSKVCSDFEEQ 219
Query: 269 VRTGITESPSKESGKMYSVVFNRWFDSQSEFNTHVQTEQFNIALS------------RNR 316
GI E E V + + S E V E+ AL R
Sbjct: 220 YADGIVEGLDNELIPSQHTVIDLDYYSTVEELVDVGPEKLKEALGALGLKVGGTPQQRAE 279
Query: 317 KRLMKEYT------FHCFSKFLSKVLQN-------------TISFTE---RKLAXXXXXX 354
+ + ++T F++ QN I+ TE +KL
Sbjct: 280 RLFLTKHTPLEKLDKKHFARPPHNGKQNGDAKSTHESENAKEIALTEAKVKKLCNLLDET 339
Query: 355 XXXXXXXXXXYESPIYAKDESEDKPSYIDNAEDTAQKESMDPTNPYNLPLGPDGFPIPRW 414
+S Y + E E + N E + E NP LP+G DG PIP W
Sbjct: 340 IERTKQNIVKKQSLTYEEMEGEREGEEA-NTELESDDEDGLIYNPLKLPIGWDGKPIPYW 398
Query: 415 LIKVQGLDVKYVCEICGNQIYHGRREFEKHFPLKKHSEGLKSLGITPSLAFKDVTTIVEV 474
L K+ GL ++ CEICGN Y GRR FE+HF +H G++ LGI + F ++T+I E
Sbjct: 399 LYKLHGLGQEFKCEICGNYSYWGRRAFERHFKEWRHQHGMRCLGIPNTKNFNEITSIEEA 458
Query: 475 TELWKALQAKSTLKSGEPFSKISTVKEPEMVLETEDNEGNVMSVQVYEELKKQGLL 530
ELWK +Q + + PE+ E ED EGN+ + + Y +L++QGL+
Sbjct: 459 KELWKRIQERQGVNKW----------RPELEEEYEDREGNIYNKKTYSDLQRQGLI 504
>7296720 [A] KOG2636 Splicing factor 3a subunit 3
Length = 503
Score = 115 bits (287), Expect = 2e-25
Identities = 64/159 (40%), Positives = 98/159 (61%), Gaps = 12/159 (7%)
Query: 372 KDESEDKPSYIDNAEDTAQKESMDPTNPYNLPLGPDGFPIPRWLIKVQGLDVKYVCEICG 431
+D+S+ + S DN +D + P NP NLPLG DG PIP WL K+ GL++ Y CEICG
Sbjct: 357 RDDSDVEASESDNEDDPDADDV--PYNPKNLPLGWDGKPIPYWLYKLHGLNISYNCEICG 414
Query: 432 NQIYHGRREFEKHFPLKKHSEGLKSLGITPSLAFKDVTTIVEVTELWKALQAKSTLKSGE 491
N Y G + F++HF +H+ G++ LGI + F +VT I + LW+ L+++ K E
Sbjct: 415 NFTYKGPKAFQRHFAEWRHAHGMRCLGIPNTAHFANVTQIEDAITLWEKLKSQ---KQSE 471
Query: 492 PFSKISTVKEPEMVLETEDNEGNVMSVQVYEELKKQGLL 530
+ ++ +E E ED+ GNV++ + +E+LK+QGLL
Sbjct: 472 RW--VADQEE-----EFEDSLGNVVNRKTFEDLKRQGLL 503
>ECU07g1140 [A] KOG2636 Splicing factor 3a subunit 3
Length = 370
Score = 66.6 bits (161), Expect = 9e-11
Identities = 37/120 (30%), Positives = 61/120 (50%), Gaps = 18/120 (15%)
Query: 411 IPRWLIKVQGLDVKYVCEICGNQIYHGRREFEKHFPLKKHSEGLKSLGITPSLAFKDVTT 470
+PRWL + + LD+ + CEICG Y GR F++HF H+ G++ G K +T
Sbjct: 269 VPRWLCRRKDLDIAFECEICGYSGY-GRDRFDRHFEEDSHARGVEMYGAKYCRVLKGITR 327
Query: 471 IVEVTELWKALQAKSTLKSGEPFSKISTVKEPEMVLETEDNEGNVMSVQVYEELKKQGLL 530
+ + ++ K+ ++ E +S+ E ED EGNV + YE+LK+ L+
Sbjct: 328 VGIL------MKMKNRMEESESYSE-----------EFEDEEGNVFDKRTYEDLKRNNLI 370
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.134 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,471,943
Number of Sequences: 60738
Number of extensions: 1212291
Number of successful extensions: 3463
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 3438
Number of HSP's gapped (non-prelim): 13
length of query: 530
length of database: 30,389,216
effective HSP length: 111
effective length of query: 419
effective length of database: 23,647,298
effective search space: 9908217862
effective search space used: 9908217862
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)