ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI6603 good J KOG2805 Translation, ribosomal structure and biogenesis tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI6603 2298224 2299468 415
(415 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL033c [J] KOG2805 tRNA (5-methylaminomethyl-2-thiouridylate)-m... 468 e-131
SPAC23H4.04 [J] KOG2805 tRNA (5-methylaminomethyl-2-thiouridylat... 211 1e-54
7290113 [J] KOG2805 tRNA (5-methylaminomethyl-2-thiouridylate)-m... 148 2e-35
At1g51310 [J] KOG2805 tRNA (5-methylaminomethyl-2-thiouridylate)... 147 4e-35
CE26926 [J] KOG2805 tRNA (5-methylaminomethyl-2-thiouridylate)-m... 136 7e-32
Hs8922248 [J] KOG2805 tRNA (5-methylaminomethyl-2-thiouridylate)... 73 7e-13
>YDL033c [J] KOG2805 tRNA
(5-methylaminomethyl-2-thiouridylate)-methyltransferase
Length = 417
Score = 468 bits (1203), Expect = e-131
Identities = 224/410 (54%), Positives = 292/410 (70%), Gaps = 9/410 (2%)
Query: 6 SRFLELCKQRRLPGYHVQSMPSKFDNIXXXXXXXXXXXXCAALFAA-YPNVHGVYMQNWG 64
+R+L L +R Y Q +P+KFDN+ AALFA +PN GVYMQNW
Sbjct: 3 ARYLNLIGRRSASPYRPQRLPAKFDNVIVAMSSGVDSSVAAALFAGEFPNTRGVYMQNWS 62
Query: 65 KDQSLTRPEEDPCYEKEWKDAEKVAKHLNIPVEFVNFEKDYWLNVFAPMLEQYSMGYTPN 124
+ QSL P ++PCYE++W+D +VAKHLNI V+ VNFE+DYW++VF PML YS G TPN
Sbjct: 63 ESQSLDDPGKEPCYERDWRDVNRVAKHLNIRVDKVNFEQDYWIDVFEPMLRGYSEGSTPN 122
Query: 125 PDIGCNQFVKFGKLMDHLDVKFGKNNYWLVMGHYARILRRDSGPERSEPHLMRGLYQQKD 184
PDIGCN+FVKFGKL + LD K+G NYWLV GHYAR+++ +G + HL+R +Y+ KD
Sbjct: 123 PDIGCNKFVKFGKLREWLDEKYGTGNYWLVTGHYARVMQEMNG--KGLFHLLRSIYRPKD 180
Query: 185 QSYYLSQVSVPSLNQMILPIGHLTKPEVREMAKEVDLHTATKPDSQGICFVNNSQHGKFK 244
QSYYLSQ++ L+ ++LPIGHLTKPEVR++AK L TA KPDSQGICFVNNSQHGKFK
Sbjct: 181 QSYYLSQINSTVLSSLLLPIGHLTKPEVRDLAKYAGLPTAEKPDSQGICFVNNSQHGKFK 240
Query: 245 NFLKEYLPMELGNIITIDELTGKKTVWGKHPGIWSYTIGQKIGISLPQGDPRYQGAWYVS 304
NFLK YLP G+IIT+D +G KT WG+H G+WSYTIGQK+GIS+PQ DP YQG W+VS
Sbjct: 241 NFLKHYLPSSPGDIITVDPQSGAKTTWGRHDGLWSYTIGQKVGISMPQADPNYQGTWFVS 300
Query: 305 EKNKRTREIVIVRGKDNNNLFKDVVTVKHFKILSKNPLSKEELNEAINKETLHMQFRSLQ 364
EK + T EI+IVRG+DN L+ D + +++F L ++ +N N L +QFRSLQ
Sbjct: 301 EKLRDTNEILIVRGRDNPALYSDTMRIENFSSLGPR---EDTINAFQNTGALTLQFRSLQ 357
Query: 365 TPVKVTSVEWINSN---EFSLKLERKERAMAPGQYCCLYVNERVIGSGII 411
PV++ S + S + ++ L K+RA+ PGQ CCLY+++RV+GSG I
Sbjct: 358 VPVQIKSCKLNRSADNLDITIHLASKQRAITPGQSCCLYIDDRVLGSGPI 407
>SPAC23H4.04 [J] KOG2805 tRNA
(5-methylaminomethyl-2-thiouridylate)-methyltransferase
Length = 415
Score = 211 bits (538), Expect = 1e-54
Identities = 130/367 (35%), Positives = 191/367 (51%), Gaps = 25/367 (6%)
Query: 54 NVHGVYMQNWGKDQSLTRPEEDPCYEKEWKDAEKVAKHLNIPVEFVNFEKDYWLNVFAPM 113
NV GV+M+NW + S P P E++W +KV K LNI NFEK+YW VF P
Sbjct: 57 NVEGVFMRNWLDEDSA--PSGCPA-ERDWATVQKVCKKLNISCRRFNFEKEYWNLVFEPS 113
Query: 114 LEQYSMGYTPNPDIGCNQFVKFGKLMDHLDVKFGKNN----YWLVMGHYARILRRDSGPE 169
L+ Y G TPNPD+ CN+ VKFG L D L K +NN +WL GHYA+ + E
Sbjct: 114 LDLYENGLTPNPDVSCNRQVKFGALFDALK-KHCENNVKGDWWLASGHYAKSV---VNIE 169
Query: 170 RSEPHLMRGLYQQKDQSYYLSQVSVPSLNQMILPIGHLTKPEVREMAKEVDL-HTATKPD 228
+E H+ ++KDQ+ +L + +L + I P+ + TK V++ A A K +
Sbjct: 170 TNESHMCIPTDKRKDQTLFLCTIRKEALEKTIFPLHNWTKENVKKQASSAGFKEIAEKQE 229
Query: 229 SQGICFVNNSQHGKFKNFLKEYLPMELGNIITIDELTGKKTVW---GKHPGIWSYTIGQK 285
SQG+CFV+ + KF+ FL+ YL + I + GK V G H GIWS T+G++
Sbjct: 230 SQGLCFVSPNVGRKFRKFLQRYLNF---SDRPIKVIAGKNVVGEFSGNH-GIWSLTVGER 285
Query: 286 IGISLPQGDPRYQGAWYVSEKNKRTREIVIVRGKDNNNLFKDVVTVKHFKILSKNPLSKE 345
G+SLPQ Y G WYV +K+ + + I RG +N L + +K +K + E
Sbjct: 286 CGLSLPQAQSEYFGRWYVWKKDIKNNALYICRGTNNELLMSKCIYLKDWKWCGTKLQNLE 345
Query: 346 ELNEAINKETLHMQFRSLQTPVKVTSVEWINSNEFSLKLERKERAMAPGQYCCLYVNERV 405
+ + H Q P++ V W N + + K+RA+ PGQ +YVN+
Sbjct: 346 KSALSCFVRVRHQQ------PLQPAKVTWRNPESVKIHFQDKQRAVTPGQVIAVYVNDVC 399
Query: 406 IGSGIID 412
+G G++D
Sbjct: 400 LGGGMVD 406
>7290113 [J] KOG2805 tRNA
(5-methylaminomethyl-2-thiouridylate)-methyltransferase
Length = 389
Score = 148 bits (373), Expect = 2e-35
Identities = 105/362 (29%), Positives = 173/362 (47%), Gaps = 29/362 (8%)
Query: 55 VHGVYMQNWGKDQSLTRPEEDPCYEKEWKDAEKVAKHLNIPVEFVNFEKDYWLNVFAPML 114
V GV+M+NW + + R E + KDAE + L + + VN+ ++YW VF+ L
Sbjct: 29 VLGVFMRNWDEADEVGRCSG----EADLKDAEWACRQLGVELRQVNYVREYWTAVFSQFL 84
Query: 115 EQYSMGYTPNPDIGCNQFVKFGKLMDHLDVKFGKNNYWLVMGHYARIL---RRDSGPERS 171
+ Y MG TPNPDI CN+ +KF H G + + GHYAR + +
Sbjct: 85 DDYQMGLTPNPDILCNRHIKFDLFHKHALENLGYDA--VATGHYARNSLGNYLEGIASNN 142
Query: 172 EPHLMRGLYQQKDQSYYLSQVSVPSLNQMILPIGHLTKPEVREMAKEVDL-HTATKPDSQ 230
+ L+ KDQ+++L+ +S +L + + P+G K +V+++AK++ A K +S
Sbjct: 143 DARLLIPADTFKDQTFFLAGISRKALQRTMFPLGDFQKSQVKDLAKKIGFQRLAKKKEST 202
Query: 231 GICFVNNSQHGKFKNFLKEYLPMELGNIITIDELTGKKTVWGKHPGIWSYTIGQKIGISL 290
GICFV FK+F++EY+ + G + ID V G H GI +T+GQ+ +S
Sbjct: 203 GICFVGKR---NFKDFIQEYITSKRGPFLDID----SGAVVGHHEGIHQWTVGQRCRLS- 254
Query: 291 PQGDPRYQGAWYVSEKNKRTREIVIVRGKDNNNLFKDVVTVKHFKILSKNPLSKEELNEA 350
+ ++V+ K + I + G ++ L H + N L +
Sbjct: 255 -----SFLQPYFVARKEAASNTIYVASGHNHPALLS-----THIAVDPPNWLCSKSQQIL 304
Query: 351 INKETLHMQFRSLQT-PVKVTSVEWINSNEFSLKLERKERAMAPGQYCCLYVNERVIGSG 409
+ +L +FR T P+ + SN F ++L+ RA+ PGQY Y + +GS
Sbjct: 305 SDTGSLRCRFRFQHTKPLVDCQLSISPSNTFLVELDAPLRAITPGQYAVFYDDTACLGSA 364
Query: 410 II 411
I
Sbjct: 365 RI 366
>At1g51310 [J] KOG2805 tRNA
(5-methylaminomethyl-2-thiouridylate)-methyltransferase
Length = 421
Score = 147 bits (370), Expect = 4e-35
Identities = 101/336 (30%), Positives = 165/336 (49%), Gaps = 32/336 (9%)
Query: 82 WKDAEKVAKHL----NIPVEFVNFEKDYWLNVFAPMLEQYSMGYTPNPDIGCNQFVKFGK 137
W+D K AKH+ ++P+E V+ +YW V + ++E+Y G TPNPD+ CN +KFG
Sbjct: 54 WEDDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIKFGA 113
Query: 138 LMDHL-DVKFGKNNYWLVMGHYARILRRDSGPERSEPHLMRGLYQQKDQSYYLSQVSVPS 196
MD + D+++ ++ GHYA+++ + + L KDQ+Y+LS +S
Sbjct: 114 FMDAISDMEYD----YVGSGHYAKVVHPPADQNDASSVLELSQDMVKDQTYFLSHLSQTQ 169
Query: 197 LNQMILPIGHLTKPEVREMAKEVDLHTATKPDSQGICFVNNSQHGKFKNFLKEYLPMELG 256
L +++ P+G + K EVR++A + DL + DSQGICF+ KF +F+ ++ G
Sbjct: 170 LKRLLFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKI---KFSDFVCRHIGEMEG 226
Query: 257 NIITIDELTGKKTVWGKHPGIWSYTIGQKIGISLPQGDPRYQGAWYVSEKNKRTREIVIV 316
I+ + G H G W YTIGQ+ G+ LP G WYV EK+ + + +
Sbjct: 227 IILEAE----SGDFLGNHRGFWFYTIGQRQGLRLP------GGPWYVVEKDTKNNVVFVS 276
Query: 317 RGKDNNNLFKDVVTVKHFKILSKNPLSKEELNEAINKETLHMQFRSLQTPVKVTSVEWIN 376
R + + + + V + S P K + E K F S S E
Sbjct: 277 RNYYSIDKRRRIFRVGSLRWNSGKPSGK--VRELRCKVRHGPGFYS-------CSFEMEG 327
Query: 377 SNEFS-LKLERKERAMAPGQYCCLYVNERVIGSGII 411
+ + + L+ ++ +A GQ+ Y IGSG+I
Sbjct: 328 DGDVAVVHLDEDDQGLAAGQFAAFYEGTTCIGSGVI 363
>CE26926 [J] KOG2805 tRNA
(5-methylaminomethyl-2-thiouridylate)-methyltransferase
Length = 375
Score = 136 bits (342), Expect = 7e-32
Identities = 112/365 (30%), Positives = 164/365 (44%), Gaps = 30/365 (8%)
Query: 54 NVHGVYMQNWGKDQSLTRPEEDPC-YEKEWKDAEKVAKHLNIPVEFVNFEKDYWLNVFAP 112
+V G++M NW + T C K+ DA V LNIP VNF K+YW +VF
Sbjct: 27 DVIGLHMINWDVQEEGT----SHCPRSKDESDARNVCDRLNIPFHTVNFVKEYWNDVFLK 82
Query: 113 MLEQYSMGYTPNPDIGCNQFVKFGKLMDHLDVKFGKNNYWLVMGHYARILRRD---SGPE 169
LE Y G T PDI CNQ +KF KF N ++ GHYA D + +
Sbjct: 83 FLENYKNGRTTVPDIDCNQSIKFDVFHKIAREKF--NADFIATGHYATTNFGDFQQNAKD 140
Query: 170 RSEPHLMRGLYQQKDQSYYLSQVSVPSLNQMILPIGHLTKPEVREMAKEVDL-HTATKPD 228
E L G KDQ+++L V+ L + + P+G L K EV+ +A+E A KP+
Sbjct: 141 SDEIRLFSGKDPLKDQTFFLCTVNQEQLKRAMFPLGSLQKSEVKRIAEEQGFQEVAKKPE 200
Query: 229 SQGICFVNNSQHGKFKNFLKEYLPMELGNIITIDELTGKKTVWGKHPGIWSYTIGQKIGI 288
S GICF+ + +F +FL EY+ + G I L + G H GI +TIG++I
Sbjct: 201 SMGICFIGKKK--RFSDFLDEYIEPKPGRI-----LLKNGSEIGNHHGIHQFTIGKRING 253
Query: 289 SLPQGDPRYQGAWYVSEKNKRTREIVIVRGKDNNNLFKDVVTVKHFK-ILSKNPLSKEEL 347
+ R ++VS + T +I+ G + +L+ + H K I + +P ++
Sbjct: 254 KYLEA--RSHLGFFVSHIHSDTGDIIACEGSHHPDLYASRFLINHPKWIRTFDPFNRISS 311
Query: 348 NEAINKETLHMQFRSLQTPVKVTSVEWINSNEFSLKLERKERAMAPGQYCCLY-VNERVI 406
N + R +T + V S+ RA APGQ C Y +
Sbjct: 312 NNFL--------CRIQRTHPPIPCVAEKQEQFLSVIPRLALRATAPGQMCVFYNTKNECL 363
Query: 407 GSGII 411
G G I
Sbjct: 364 GGGEI 368
>Hs8922248 [J] KOG2805 tRNA
(5-methylaminomethyl-2-thiouridylate)-methyltransferase
Length = 166
Score = 73.2 bits (178), Expect = 7e-13
Identities = 41/112 (36%), Positives = 61/112 (53%), Gaps = 8/112 (7%)
Query: 55 VHGVYMQNWGKDQSLTRPEEDPCY-EKEWKDAEKVAKHLNIPVEFVNFEKDYWLNVFAPM 113
V GV+M+NW + E C +K+ +DA +V + L+IP V++ K+YW +VF+
Sbjct: 31 VTGVFMKNWD-----SLDEHGVCTADKDCEDAYRVCQILDIPFHQVSYVKEYWNDVFSDF 85
Query: 114 LEQYSMGYTPNPDIGCNQFVKFGKLMDHLDVKFGKNNYWLVMGHYARILRRD 165
L +Y G TPNPDI CN+ +KF + G + + GHYAR D
Sbjct: 86 LNEYEKGRTPNPDIVCNKHIKFSCFFHYAVDNLGADA--IATGHYARTSLED 135
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.137 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,661,816
Number of Sequences: 60738
Number of extensions: 1185390
Number of successful extensions: 2688
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2659
Number of HSP's gapped (non-prelim): 7
length of query: 415
length of database: 30,389,216
effective HSP length: 109
effective length of query: 306
effective length of database: 23,768,774
effective search space: 7273244844
effective search space used: 7273244844
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)