ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI6631 good A KOG1817 RNA processing and modification Ribonuclease
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI6631 2308342 2309775 478
(478 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YMR239c [A] KOG1817 Ribonuclease 412 e-115
SPBC119.11c [A] KOG1817 Ribonuclease 118 2e-26
CE29039 [A] KOG1817 Ribonuclease 62 2e-09
7304132 [A] KOG1817 Ribonuclease 57 6e-08
Hs21359822 [A] KOG1817 Ribonuclease 53 9e-07
HsM7106397 [A] KOG1817 Ribonuclease 52 1e-06
>YMR239c [A] KOG1817 Ribonuclease
Length = 471
Score = 412 bits (1060), Expect = e-115
Identities = 212/427 (49%), Positives = 291/427 (67%), Gaps = 14/427 (3%)
Query: 40 YTYSQIIKLEHSITDLVKQLKTVIELSPNIKQYHQALDQLPTMSSDLIPSFDRYQLKLAA 99
Y Y ++I+LEH++T LV+ +IELSPN+ Y++A++ + ++PS RYQLKLAA
Sbjct: 44 YKYLEVIQLEHAVTKLVESYNKIIELSPNLVAYNEAVNNQDRVPVQILPSLSRYQLKLAA 103
Query: 100 ELTSLYQLGKLELFNNIIHYSDKFKSGSSTPILPELQN------ENLDLLSANVTKSLHI 153
EL +L+ L K + I Y ++F + PIL E+ E L+ + + + +
Sbjct: 104 ELKTLHDLKKDAILTEITDYENEFDTEQKQPILQEISKADMEKLEKLEQVKREKREKIDV 163
Query: 154 NSHENSKATXXXXXXXXXXXGYEVID-NAVTDLKKGKEWPPKLPKIKNPVIRARVFIHKS 212
N +EN G + D D+ K +WPPKLP+I++ IRARVFIHKS
Sbjct: 164 NVYENLNEKEDEEEDE----GEDSYDPTKAGDIVKATKWPPKLPEIQDLAIRARVFIHKS 219
Query: 213 MINDKLYLTDQEIIRSHNERLEFLGDSILNTTMTTIIYNKFPHFDEGQLSQLRMRLINNE 272
I DK+YL+ E+I +HNERLEFLGDSILN+ MT IIYNKFP + EGQLS LRM L++NE
Sbjct: 220 TIKDKVYLSGSEMINAHNERLEFLGDSILNSVMTLIIYNKFPDYSEGQLSTLRMNLVSNE 279
Query: 273 LLKEWSFLYKFPDELKTHNVKPDDN---KDGKQKLYADVFEAYIGGLVEDNGKKNLPKIR 329
+K+WS +Y F ++LKT+ D+N ++GK KLYADVFEAYIGGL+ED+ + NLPKIR
Sbjct: 280 QIKQWSIMYNFHEKLKTNFDLKDENSNFQNGKLKLYADVFEAYIGGLMEDDPRNNLPKIR 339
Query: 330 KWLAKLAEPVISKGLNSDITLENTTEVDVNAKKTLYSLIGYAALNLHYYPVHKPTSTDPN 389
KWL KLA+PVI + + + LE T ++D+NAK+ LYSLIGYA+L LHY V KPT+ DPN
Sbjct: 340 KWLRKLAKPVIEEATRNQVALEKTDKLDMNAKRQLYSLIGYASLRLHYVTVKKPTAVDPN 399
Query: 390 AIVECRITGGKVLGRGVGKNIKIAGMRAAEDVLNNKELIEKYVSLRASLPKSESVIKANN 449
+IVECR+ G VLG GVG+NIKIAG+RAAE+ L +K++++ Y RA++P+SESV+K +
Sbjct: 400 SIVECRVGDGTVLGTGVGRNIKIAGIRAAENALRDKKMLDFYAKQRAAIPRSESVLKDPS 459
Query: 450 DNTKKRK 456
KKRK
Sbjct: 460 QKNKKRK 466
>SPBC119.11c [A] KOG1817 Ribonuclease
Length = 363
Score = 118 bits (295), Expect = 2e-26
Identities = 83/239 (34%), Positives = 125/239 (51%), Gaps = 22/239 (9%)
Query: 190 EWPPKLPKIKNPVIRARVFIHKSMINDKLYLTDQ---EIIRSHNERLEFLGDSILNTTMT 246
E+PP LP +++ ++ +VF+H S + +Y E++ HNERLEFLGDS N T
Sbjct: 129 EYPPPLPPLRSEKLKEQVFMHISRAYE-IYPNQSNPNELLDIHNERLEFLGDSFFNLFTT 187
Query: 247 TIIYNKFPHFDEGQLSQLRMRLINNELLKEWSFLYKFPDELKTHNVKPDDNKDGKQKLYA 306
II++KFP DEG LS+LR + + NE +++ LY F L D QK+ A
Sbjct: 188 RIIFSKFPQMDEGSLSKLRAKFVGNESADKFARLYGFDKTLVLSYSAEKDQLRKSQKVIA 247
Query: 307 DVFEAYIGGLVEDNGKKNLPKIRKWLAKLAEPVISKGLNSDITLENTTEVDVNAKKTL-- 364
D FEAY+G L+ D ++ +W+++L +P I ++IT++ +D AK L
Sbjct: 248 DTFEAYLGALILDGQEET---AFQWVSRLLQPKI-----ANITVQR--PIDKLAKSKLFH 297
Query: 365 -YSLIGYAALNLHYYPVHKPTSTDPNAIVECRITGGKVLGRGVGKNIKIAGMRAAEDVL 422
YS +G+ + Y V + ++ C I GK + R G N K AG RAA L
Sbjct: 298 KYSTLGH----IEYRWVDGAGGSAEGYVIAC-IFNGKEVARAWGANQKDAGSRAAMQAL 351
>CE29039 [A] KOG1817 Ribonuclease
Length = 1086
Score = 61.6 bits (148), Expect = 2e-09
Identities = 37/109 (33%), Positives = 58/109 (52%), Gaps = 17/109 (15%)
Query: 220 LTDQEIIRSHNERLEFLGDSILNTTMTTIIYNKFPHFDEGQLSQLRMRLINNEL------ 273
+ + ++ + HN+RLE+LGDS+L ++ +Y +FP+ EG +S LR L++N+
Sbjct: 859 IPNNDLTKGHNQRLEWLGDSVLQLIVSDFLYRRFPYHHEGHMSLLRTSLVSNQTQAVVCD 918
Query: 274 -LKEWSFLYKFPDELKTHNVKPDDNKDGKQKLYADVFEAYIGGLVEDNG 321
L F+ K P KT +K D AD+ EA+IG L D G
Sbjct: 919 DLGFTEFVIKAP--YKTPELKLKDK--------ADLVEAFIGALYVDRG 957
>7304132 [A] KOG1817 Ribonuclease
Length = 1327
Score = 57.0 bits (136), Expect = 6e-08
Identities = 70/276 (25%), Positives = 113/276 (40%), Gaps = 25/276 (9%)
Query: 197 KIKNPVIRARVFIHKSMINDKLYLTDQEIIRSHNERLEFLGDSILNTTMTTIIYNKFPHF 256
K K+ + AR F +S+ L L N+RLEFLGD++L + +Y FP
Sbjct: 985 KFKHIRLLARAFTDRSIGFTHLTL-------GSNQRLEFLGDTVLQLICSEYLYRHFPEH 1037
Query: 257 DEGQLSQLRMRLINNELLKEWSFLYKFPDELKTHNVKPDDNKDGKQKLYADVFEAYIGGL 316
EG LS LR L+NN P + V + D K K AD+ EA++G L
Sbjct: 1038 HEGHLSLLRSSLVNNRTQAVVCDDLGMP----KYAVYANPKADLKTKDRADLLEAFLGAL 1093
Query: 317 VEDNGKKNLPKIRKWLAKLAEPVISKGLNSDITLENTTEVDVNAKK---TLYSLIGYAAL 373
D G + + + L I ++ + ++ TL ++ G
Sbjct: 1094 YVDKG-------LLYCEQFCHVCLFPRLQLFIMNQDWNDPKSKLQQCCLTLRTMDG-GEP 1145
Query: 374 NLHYYPVHKPTSTDPNAIVECRI-TGGKVLGRGVGKNIKIAGMRAAEDVL-NNKELIEKY 431
++ YY V + + + + + K L G +I+ A M AA+ L N+++L +
Sbjct: 1146 DIPYYKVVEASGPTNTRVYKVAVYFRSKRLATSSGSSIQQAEMNAAKQALENSRDLFPQL 1205
Query: 432 VSLRASLPKSESVIKANN-DNTKKRKHEGHSNDRGK 466
+ + KS N DN R+H+ R K
Sbjct: 1206 DHQKRVIAKSIKKQTGNELDNDSDRQHQEEKIKRPK 1241
>Hs21359822 [A] KOG1817 Ribonuclease
Length = 1374
Score = 53.1 bits (126), Expect = 9e-07
Identities = 60/213 (28%), Positives = 91/213 (42%), Gaps = 44/213 (20%)
Query: 229 HNERLEFLGDSILNTTMTTIIYNKFPHFDEGQLSQLRMRLINNELLKEWSFLYKFPDELK 288
HN+R+EFLGDSI+ T ++ FP EG L+ LR L+NN K +EL
Sbjct: 1142 HNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQA------KVAEELG 1195
Query: 289 THNVKPDDNKDG-----KQKLYADVFEAYIGGLVEDNGKKNLPKIRKWL-----AKLAEP 338
++K + K AD+ E++I L D K+L + ++ +L E
Sbjct: 1196 MQEYAITNDKTKRPVALRTKTLADLLESFIAALYTD---KDLEYVHTFMNVCFFPRLKEF 1252
Query: 339 VISKGLNSD--------ITLENT-TEVDVNAKKTLYSLIGYAALNLHYYPVHKPTSTDPN 389
++++ N +TL E D+ KTL ++ P H T T
Sbjct: 1253 ILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTV----------GPSHARTYT--- 1299
Query: 390 AIVECRITGGKVLGRGVGKNIKIAGMRAAEDVL 422
V G ++ G G G +I+ A M AA D L
Sbjct: 1300 --VAVYFKGERI-GCGKGPSIQQAEMGAAMDAL 1329
>HsM7106397 [A] KOG1817 Ribonuclease
Length = 541
Score = 52.4 bits (124), Expect = 1e-06
Identities = 60/213 (28%), Positives = 91/213 (42%), Gaps = 44/213 (20%)
Query: 229 HNERLEFLGDSILNTTMTTIIYNKFPHFDEGQLSQLRMRLINNELLKEWSFLYKFPDELK 288
HN+R+EFLGDSI+ T ++ FP EG L+ LR L+NN K +EL
Sbjct: 309 HNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQA------KVAEELG 362
Query: 289 THNVKPDDNKDG-----KQKLYADVFEAYIGGLVEDNGKKNLPKIRKWL-----AKLAEP 338
++K + K AD+ E++I L D K+L + ++ +L E
Sbjct: 363 MQEYAITNDKTKRPVALRTKTLADLLESFIAALYID---KDLEYVHTFMNVCFFPRLKEF 419
Query: 339 VISKGLNSD--------ITLENT-TEVDVNAKKTLYSLIGYAALNLHYYPVHKPTSTDPN 389
++++ N +TL E D+ KTL ++ P H T T
Sbjct: 420 ILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTV----------GPSHARTYT--- 466
Query: 390 AIVECRITGGKVLGRGVGKNIKIAGMRAAEDVL 422
V G ++ G G G +I+ A M AA D L
Sbjct: 467 --VAVYFKGERI-GCGKGPSIQQAEMGAAMDAL 496
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.134 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,306,565
Number of Sequences: 60738
Number of extensions: 1229025
Number of successful extensions: 2719
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 2706
Number of HSP's gapped (non-prelim): 10
length of query: 478
length of database: 30,389,216
effective HSP length: 110
effective length of query: 368
effective length of database: 23,708,036
effective search space: 8724557248
effective search space used: 8724557248
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)