ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI6781 good U KOG3229 Intracellular trafficking, secretion, and vesicular transport Vacuolar sorting protein VPS24
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI6781 2361279 2361950 224
(224 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKL041w [U] KOG3229 Vacuolar sorting protein VPS24 210 1e-54
SPAC9E9.14 [U] KOG3229 Vacuolar sorting protein VPS24 102 3e-22
At5g22950 [U] KOG3229 Vacuolar sorting protein VPS24 85 7e-17
7296875 [U] KOG3229 Vacuolar sorting protein VPS24 84 1e-16
CE27211 [U] KOG3229 Vacuolar sorting protein VPS24 69 7e-12
Hs7706353 [U] KOG3229 Vacuolar sorting protein VPS24 64 2e-10
At3g45000 [U] KOG3229 Vacuolar sorting protein VPS24 62 5e-10
YKL002w [U] KOG3230 Vacuolar assembly/sorting protein DID4 52 5e-07
CE21987 [U] KOG3230 Vacuolar assembly/sorting protein DID4 52 7e-07
Hs7656922 [U] KOG3230 Vacuolar assembly/sorting protein DID4 50 2e-06
At2g06530 [U] KOG3230 Vacuolar assembly/sorting protein DID4 49 4e-06
>YKL041w [U] KOG3229 Vacuolar sorting protein VPS24
Length = 224
Score = 210 bits (535), Expect = 1e-54
Identities = 115/225 (51%), Positives = 145/225 (64%), Gaps = 4/225 (1%)
Query: 1 MNFIKTAIWGPDPKEQHRKLKSILRKNDRQXXXXXXXXXXXXXXTQQLIKQAAKQNDIKT 60
M++IK AIWGPDPKEQ R+++S+LRKN R TQQLIK++AK+ND++T
Sbjct: 1 MDYIKKAIWGPDPKEQQRRIRSVLRKNGRNIEKSLRELTVLQNKTQQLIKKSAKKNDVRT 60
Query: 61 VRLYAKELYHVNKQYNRMYTSKAQLQSVGMKIEECFQMNKLQDKMAQSAVLMRDVNSLVR 120
VRLYAKELY +NKQY+RMYTS+AQL SV MKI+E +MN L ++MA SA LMR+VNSLVR
Sbjct: 61 VRLYAKELYQINKQYDRMYTSRAQLDSVRMKIDEAIRMNTLSNQMADSAGLMREVNSLVR 120
Query: 121 LPQLRGTMIELEKELVKSGIIT--XXXXXXXXXXXXXXXXXXXXXXXXQIVAEYTSEKLG 178
LPQLR TMIELEKEL+KSGII+ +IV +YT+EK
Sbjct: 121 LPQLRNTMIELEKELMKSGIISEMVDDTMESVGDVGEEMDEAVDEEVNKIVEQYTNEKFK 180
Query: 179 KVEETPNVVLSXXXXXXXXXXXXSNIDAEADNMIKAMKERLNALQ 223
V++ P V L+ +D EAD M+ M+ERL ALQ
Sbjct: 181 NVDQVPTVELA--ANEEEQEIPDEKVDEEADRMVNEMRERLRALQ 223
>SPAC9E9.14 [U] KOG3229 Vacuolar sorting protein VPS24
Length = 231
Score = 102 bits (255), Expect = 3e-22
Identities = 54/141 (38%), Positives = 83/141 (58%)
Query: 1 MNFIKTAIWGPDPKEQHRKLKSILRKNDRQXXXXXXXXXXXXXXTQQLIKQAAKQNDIKT 60
M +++ +GP P+EQ+RK +SI+RK RQ + +KQ AKQ+DI
Sbjct: 1 MQTVRSYFFGPTPQEQNRKWQSIIRKEQRQLDRQVYHLKAGRKKAEVQLKQLAKQSDITN 60
Query: 61 VRLYAKELYHVNKQYNRMYTSKAQLQSVGMKIEECFQMNKLQDKMAQSAVLMRDVNSLVR 120
+R+ AKE+ N+ R+ SKA L S+ +++ + M K+Q M S +M+DV+SL+R
Sbjct: 61 MRILAKEIARANRHGKRLAESKALLGSLSLQLNDQMAMLKIQGTMQSSTKIMQDVSSLIR 120
Query: 121 LPQLRGTMIELEKELVKSGII 141
LPQL TM L EL K+G++
Sbjct: 121 LPQLSETMRNLSMELTKAGVL 141
>At5g22950 [U] KOG3229 Vacuolar sorting protein VPS24
Length = 229
Score = 85.1 bits (209), Expect = 7e-17
Identities = 48/141 (34%), Positives = 78/141 (55%), Gaps = 3/141 (2%)
Query: 1 MNFIKTAIWGPDPKEQHRKLKSILRKNDRQXXXXXXXXXXXXXXTQQLIKQAAKQNDIKT 60
MN IK PDPK+ R + LR+ R Q+ IK+AAK+ND+ +
Sbjct: 5 MNIIKPK---PDPKQLLRDWQRKLRQECRNIERQIRDIQKEERNVQKAIKEAAKRNDMVS 61
Query: 61 VRLYAKELYHVNKQYNRMYTSKAQLQSVGMKIEECFQMNKLQDKMAQSAVLMRDVNSLVR 120
+ AKE+ + NR+Y +KAQ+ S+ M + E + + +++SA +M+ VN+L++
Sbjct: 62 AKALAKEIVSSRRTVNRLYENKAQMNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLMK 121
Query: 121 LPQLRGTMIELEKELVKSGII 141
PQ+ TM E KE+ K+G+I
Sbjct: 122 APQMAATMQEFSKEMTKAGVI 142
>7296875 [U] KOG3229 Vacuolar sorting protein VPS24
Length = 223
Score = 84.3 bits (207), Expect = 1e-16
Identities = 56/213 (26%), Positives = 97/213 (45%), Gaps = 2/213 (0%)
Query: 12 DPKEQHRKLKSILRKNDRQXXXXXXXXXXXXXXTQQLIKQAAKQNDIKTVRLYAKELYHV 71
DPKEQ ++ +RK Q ++ +KQAA++ND T + AKE+ +
Sbjct: 11 DPKEQVQEWTHKIRKEGNQLDRQIRSIQREEEKVKRSLKQAAQKNDRDTCVILAKEIVNA 70
Query: 72 NKQYNRMYTSKAQLQSVGMKIEECFQMNKLQDKMAQSAVLMRDVNSLVRLPQLRGTMIEL 131
K NR+YTSKA L S+ M+++ ++ + +S +++ + SLVR P+L G M ++
Sbjct: 71 RKAINRIYTSKAHLNSIQMQMKNQLSTLRVAGSLQKSTEVLQAMQSLVRYPELAGIMRDM 130
Query: 132 EKELVKSGII-TXXXXXXXXXXXXXXXXXXXXXXXXQIVAEYTSEKLGKVEETPNVVLSX 190
KE++K+GII +++ E T KLG+ P +
Sbjct: 131 SKEMMKAGIIEEMLDETMDSLEESEELEEEASKEVDKVLWEITDGKLGEAPLPPEATPAD 190
Query: 191 XXXXXXXXXXXSNIDAEADNMIKAMKERLNALQ 223
D E + ++ M+ RL +L+
Sbjct: 191 KASASAARVEAEQDDEEGEE-LQEMQSRLASLR 222
>CE27211 [U] KOG3229 Vacuolar sorting protein VPS24
Length = 208
Score = 68.6 bits (166), Expect = 7e-12
Identities = 44/168 (26%), Positives = 77/168 (45%)
Query: 12 DPKEQHRKLKSILRKNDRQXXXXXXXXXXXXXXTQQLIKQAAKQNDIKTVRLYAKELYHV 71
DPKE+ R+L+ +R+ R + IK+AAK+ D + AK +
Sbjct: 9 DPKEEVRELQRKMRQEMRSLDRQVYSIQREEQKVTKEIKEAAKKGDKDVCLILAKSMIQS 68
Query: 72 NKQYNRMYTSKAQLQSVGMKIEECFQMNKLQDKMAQSAVLMRDVNSLVRLPQLRGTMIEL 131
K ++++ SKAQ+ SV M ++E ++ + +S +M+ + LV++P++ TM E+
Sbjct: 69 RKAISKIHVSKAQINSVIMCMQEQLATIRMAGSLQKSTEVMKSMQQLVKVPEIMKTMREM 128
Query: 132 EKELVKSGIITXXXXXXXXXXXXXXXXXXXXXXXXQIVAEYTSEKLGK 179
E++K GII I+ E T +LGK
Sbjct: 129 SAEMMKLGIIEEMIEETLESVEPAGLEEKAQEEVENILWEVTQGELGK 176
>Hs7706353 [U] KOG3229 Vacuolar sorting protein VPS24
Length = 222
Score = 63.5 bits (153), Expect = 2e-10
Identities = 37/129 (28%), Positives = 67/129 (51%)
Query: 13 PKEQHRKLKSILRKNDRQXXXXXXXXXXXXXXTQQLIKQAAKQNDIKTVRLYAKELYHVN 72
PKE + +RK R ++ +K AAK+ + AKE+
Sbjct: 12 PKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLAKEMIRSR 71
Query: 73 KQYNRMYTSKAQLQSVGMKIEECFQMNKLQDKMAQSAVLMRDVNSLVRLPQLRGTMIELE 132
K +++Y SKA + SV M ++ + ++ + +S +M+ + SLV++P+++ TM EL
Sbjct: 72 KAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQATMRELS 131
Query: 133 KELVKSGII 141
KE++K+GII
Sbjct: 132 KEMMKAGII 140
>At3g45000 [U] KOG3229 Vacuolar sorting protein VPS24
Length = 200
Score = 62.4 bits (150), Expect = 5e-10
Identities = 30/93 (32%), Positives = 54/93 (57%)
Query: 49 IKQAAKQNDIKTVRLYAKELYHVNKQYNRMYTSKAQLQSVGMKIEECFQMNKLQDKMAQS 108
IK + K+ND+ T + A+E+ + R+Y +KAQ+ S+ M + E +++S
Sbjct: 21 IKDSVKRNDLVTAKALAREIVSSRRTVKRLYENKAQVNSISMHLGESIATAVTVGNLSKS 80
Query: 109 AVLMRDVNSLVRLPQLRGTMIELEKELVKSGII 141
+M+ VNSL++ P++ TM KE+ K+G+I
Sbjct: 81 GEVMKLVNSLMKAPEIAVTMQAFSKEMTKTGVI 113
>YKL002w [U] KOG3230 Vacuolar assembly/sorting protein DID4
Length = 232
Score = 52.4 bits (124), Expect = 5e-07
Identities = 28/122 (22%), Positives = 58/122 (46%)
Query: 13 PKEQHRKLKSILRKNDRQXXXXXXXXXXXXXXTQQLIKQAAKQNDIKTVRLYAKELYHVN 72
P+E+ +K + L + R+ IK++AK + ++ AK+L
Sbjct: 14 PQERLKKNQRALERTQRELEREKRKLELQDKKLVSEIKKSAKNGQVAAAKVQAKDLVRTR 73
Query: 73 KQYNRMYTSKAQLQSVGMKIEECFQMNKLQDKMAQSAVLMRDVNSLVRLPQLRGTMIELE 132
+ KAQLQ++ ++I+ +++ M+++ L+ +N + LPQL+ +E E
Sbjct: 74 NYIQKFDNMKAQLQAISLRIQAVRSSDQMTRSMSEATGLLAGMNRTMNLPQLQRISMEFE 133
Query: 133 KE 134
K+
Sbjct: 134 KQ 135
>CE21987 [U] KOG3230 Vacuolar assembly/sorting protein DID4
Length = 237
Score = 52.0 bits (123), Expect = 7e-07
Identities = 29/121 (23%), Positives = 56/121 (45%), Gaps = 4/121 (3%)
Query: 14 KEQHRKLKSILRKNDRQXXXXXXXXXXXXXXTQQLIKQAAKQNDIKTVRLYAKELYHVNK 73
++ R L +R+ DR+ IK AK+N + +V++ AK+L +
Sbjct: 16 RQNQRALNKAIRELDRERARMEAQEKKVIAD----IKNMAKKNQMDSVKVMAKDLVRTRR 71
Query: 74 QYNRMYTSKAQLQSVGMKIEECFQMNKLQDKMAQSAVLMRDVNSLVRLPQLRGTMIELEK 133
+ KA +Q+V +K++ + + M M+ +N + LPQ++ M+E EK
Sbjct: 72 YIKKFIVMKANIQAVSLKVQTLKSQDAMASAMKGVTKAMQSMNRQLNLPQIQKIMMEFEK 131
Query: 134 E 134
+
Sbjct: 132 Q 132
>Hs7656922 [U] KOG3230 Vacuolar assembly/sorting protein DID4
Length = 222
Score = 50.4 bits (119), Expect = 2e-06
Identities = 30/121 (24%), Positives = 55/121 (44%), Gaps = 4/121 (3%)
Query: 14 KEQHRKLKSILRKNDRQXXXXXXXXXXXXXXTQQLIKQAAKQNDIKTVRLYAKELYHVNK 73
++ R L +R+ DR+ IK+ AKQ + VR+ AK+L +
Sbjct: 16 RQNQRALNRAMRELDRERQKLETQEKKIIAD----IKKMAKQGQMDAVRIMAKDLVRTRR 71
Query: 74 QYNRMYTSKAQLQSVGMKIEECFQMNKLQDKMAQSAVLMRDVNSLVRLPQLRGTMIELEK 133
+ +A +Q+V +KI+ N + M M +N ++LPQ++ M+E E+
Sbjct: 72 YVRKFVLMRANIQAVSLKIQTLKSNNSMAQAMKGVTKAMGTMNRQLKLPQIQKIMMEFER 131
Query: 134 E 134
+
Sbjct: 132 Q 132
>At2g06530 [U] KOG3230 Vacuolar assembly/sorting protein DID4
Length = 218
Score = 49.3 bits (116), Expect = 4e-06
Identities = 27/94 (28%), Positives = 47/94 (49%)
Query: 49 IKQAAKQNDIKTVRLYAKELYHVNKQYNRMYTSKAQLQSVGMKIEECFQMNKLQDKMAQS 108
IK+ AKQ + V++ AK+L Q + Y K+QLQ V ++I+ + + M
Sbjct: 41 IKKTAKQGQMGAVKVMAKDLIRTRHQIEKFYKLKSQLQGVSLRIQTLKSTQAMGEAMKGV 100
Query: 109 AVLMRDVNSLVRLPQLRGTMIELEKELVKSGIIT 142
M +N + LP L+ M E E++ K +++
Sbjct: 101 TKAMGQMNRQMNLPSLQKIMQEFERQNEKMEMVS 134
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.314 0.129 0.342
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,465,814
Number of Sequences: 60738
Number of extensions: 217483
Number of successful extensions: 647
Number of sequences better than 1.0e-05: 11
Number of HSP's better than 0.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 633
Number of HSP's gapped (non-prelim): 11
length of query: 224
length of database: 30,389,216
effective HSP length: 102
effective length of query: 122
effective length of database: 24,193,940
effective search space: 2951660680
effective search space used: 2951660680
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)