ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI6799 good O KOG2358 Posttranslational modification, protein turnover, chaperones NifU-like domain-containing proteins
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI6799 2368507 2367740 -256
(256 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKL040c [O] KOG2358 NifU-like domain-containing proteins 293 1e-79
At3g20970 [O] KOG2358 NifU-like domain-containing proteins 134 1e-31
At1g51390 [O] KOG2358 NifU-like domain-containing proteins 127 2e-29
Hs7661720 [O] KOG2358 NifU-like domain-containing proteins 121 1e-27
CE06292 [O] KOG2358 NifU-like domain-containing proteins 120 2e-27
SPBC1709.19c [O] KOG2358 NifU-like domain-containing proteins 102 4e-22
At4g25910 [O] KOG2358 NifU-like domain-containing proteins 57 3e-08
At4g01940 [O] KOG2358 NifU-like domain-containing proteins 54 2e-07
At5g49940_1 [O] KOG2358 NifU-like domain-containing proteins 52 1e-06
>YKL040c [O] KOG2358 NifU-like domain-containing proteins
Length = 256
Score = 293 bits (751), Expect = 1e-79
Identities = 136/229 (59%), Positives = 185/229 (80%), Gaps = 5/229 (2%)
Query: 12 YKSVHRTVLNPVW----QRSIHIKTLTTPNENALKFVSTDGGLLQEKGTQSVEIKNTDEE 67
+KSV + +P++ QR IHIKTLTTPNENALKF+STDG +LQ +G++S+ IKNTDE
Sbjct: 2 FKSVAKLGKSPIFYLNSQRLIHIKTLTTPNENALKFLSTDGEMLQTRGSKSIVIKNTDEN 61
Query: 68 LLKHAPFPSKVFQQCPGVEAMMIGDDFVTINKDELIHWNQVTPTVIDLLVQHLASGKPTF 127
L+ H+ ++F QCPGVE++MIGDDF+TINKD ++HWN + P +IDLL + LA G+
Sbjct: 62 LINHSKLAQQIFLQCPGVESLMIGDDFLTINKDRMVHWNSIKPEIIDLLTKQLAYGEDVI 121
Query: 128 LPDFFDVKKSS-EVGYDVDIPKFEYDEDEQEISEMIDELIQTRIRPAIMDDGGDIQYRGW 186
+F V++ E GY +++PKFE E+++E+SE+I+ELI TRIRPAI++DGGDI YRGW
Sbjct: 122 SKEFHAVQEEEGEGGYKINMPKFELTEEDEEVSELIEELIDTRIRPAILEDGGDIDYRGW 181
Query: 187 NPETGTVYLKLQGACKSCSSSEDTLKHGIEAMLKHYIEEVEDVVQILDP 235
+P+TGTVYL+LQGAC SCSSSE TLK+GIE+MLKHY++EV++V+QI+DP
Sbjct: 182 DPKTGTVYLRLQGACTSCSSSEVTLKYGIESMLKHYVDEVKEVIQIMDP 230
>At3g20970 [O] KOG2358 NifU-like domain-containing proteins
Length = 283
Score = 134 bits (338), Expect = 1e-31
Identities = 79/210 (37%), Positives = 116/210 (54%), Gaps = 18/210 (8%)
Query: 25 QRSIHIKTLTTPNENALKFVSTDGGLLQEKGTQSVEIKNTDEELLKHAPFPSKVFQQCPG 84
+R++ I+T +TPN ++L F G + E G S + N L +P ++ G
Sbjct: 77 RRTMFIQTQSTPNPSSLMFYP--GKPVMEVG--SADFPNVRSAL--GSPLAKSIYS-IDG 129
Query: 85 VEAMMIGDDFVTINKDELIHWNQVTPTVIDLLVQHLASGKPTFLPDFFDVKKSSEVGYDV 144
V + G DFVT+ K + + W+ + P + ++ +SG+P FL K + +
Sbjct: 130 VVRVFFGSDFVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQAAAAKDTAIS--- 186
Query: 145 DIPKFEYDEDEQEISEMIDELIQTRIRPAIMDDGGDIQYRGWNPETGTVYLKLQGACKSC 204
ED+ E MI EL++TRIRPA+ DDGGDI+Y G++PE+G V L++QGAC C
Sbjct: 187 --------EDDSETVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSGC 238
Query: 205 SSSEDTLKHGIEAMLKHYIEEVEDVVQILD 234
SS TLK GIE ML HY+ EV+ V Q D
Sbjct: 239 PSSSVTLKSGIENMLMHYVSEVKGVEQEFD 268
>At1g51390 [O] KOG2358 NifU-like domain-containing proteins
Length = 304
Score = 127 bits (319), Expect = 2e-29
Identities = 80/234 (34%), Positives = 119/234 (50%), Gaps = 37/234 (15%)
Query: 25 QRSIHIKTLTTPNENALKFVSTDGGLLQEKGTQSVE----------------IKNTDEEL 68
+R++ I+T +TPN ++L F + G + E G+ I L
Sbjct: 72 RRTMFIQTQSTPNPSSLMF--SPGKPVMEIGSADFPNSRSAMSSPLAKAIFAIDGIPRLL 129
Query: 69 LKHAP--------FPSKVFQQCPGVEAMMIGDDFVTINKDELIHWNQVTPTVIDLLVQHL 120
L+H F +K+ GV + G DFVT+ K + + W+ + P + +++
Sbjct: 130 LQHTIVSSSYNPCFVTKIVSVDAGVVRVFYGSDFVTVTKSDDVTWDILKPDIFAVVMDFY 189
Query: 121 ASGKPTFLPDFFDVKKSSEVGYDVDIPKFEYDEDEQEISEMIDELIQTRIRPAIMDDGGD 180
+SG+P FL K + + ED+ E MI EL++TRIRP++ DDGGD
Sbjct: 190 SSGQPLFLDSQATAAKDTAI-----------HEDDSETVAMIKELLETRIRPSVQDDGGD 238
Query: 181 IQYRGWNPETGTVYLKLQGACKSCSSSEDTLKHGIEAMLKHYIEEVEDVVQILD 234
I+Y G++ ETG V L++QGAC C SS TLK GIE ML HY+ EV+ V Q D
Sbjct: 239 IEYCGFDTETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVSEVKGVEQEFD 292
>Hs7661720 [O] KOG2358 NifU-like domain-containing proteins
Length = 196
Score = 121 bits (303), Expect = 1e-27
Identities = 79/214 (36%), Positives = 114/214 (52%), Gaps = 40/214 (18%)
Query: 30 IKTLTTPNENALKFVSTDGGLLQEKGTQSVEIKNTDEELLKHAPFPSKVFQ--------Q 81
I+T TPN N+LKF+ G +E + D P P+ F+ +
Sbjct: 3 IQTQDTPNPNSLKFIP---------GKPVLETRTMD------FPTPAAAFRSPLARQLFR 47
Query: 82 CPGVEAMMIGDDFVTINKD-ELIHWNQVTPTVIDLLVQHLASGKPTFLPDFFDVKKSSEV 140
GV+++ G DF+T+ K+ E + WN + P + ++ ASG LP + S E
Sbjct: 48 IEGVKSVFFGPDFITVTKENEELDWNLLKPDIYATIMDFFASG----LPLVTEETPSGEA 103
Query: 141 GYDVDIPKFEYDEDEQEISEMIDELIQTRIRPAIMDDGGDIQYRGWNPETGTVYLKLQGA 200
G E++ E+ MI EL+ TRIRP + +DGGD+ Y+G+ E G V LKLQG+
Sbjct: 104 G----------SEEDDEVVAMIKELLDTRIRPTVQEDGGDVIYKGF--EDGIVQLKLQGS 151
Query: 201 CKSCSSSEDTLKHGIEAMLKHYIEEVEDVVQILD 234
C SC SS TLK+GI+ ML+ YI EVE V Q++D
Sbjct: 152 CTSCPSSIITLKNGIQNMLQFYIPEVEGVEQVMD 185
>CE06292 [O] KOG2358 NifU-like domain-containing proteins
Length = 222
Score = 120 bits (301), Expect = 2e-27
Identities = 72/210 (34%), Positives = 118/210 (55%), Gaps = 23/210 (10%)
Query: 26 RSIHIKTLTTPNENALKFVSTDGGLLQEKGTQSVEIKNTDEELLKHAPFPSKVFQQCPGV 85
RS++I+ TPN +LKF+ G L +++ + + K +P K+ + GV
Sbjct: 14 RSMYIQVQETPNPLSLKFLP--GQQLLPDASKTYDFSSA--ATAKQSPLAIKLLR-VDGV 68
Query: 86 EAMMIGDDFVTINK-DELIHWNQVTPTVIDLLVQHLASGKPTFLPDFFDVKKSSEVGYDV 144
+ + G+DFVT+ K DE + W + P + + H+ +GKP + +++ V V
Sbjct: 69 KRVFFGEDFVTVTKSDETVDWALLRPEIFSTIADHIQTGKPV-------INEAATVSDQV 121
Query: 145 DIPKFEYDEDEQEISEMIDELIQTRIRPAIMDDGGDIQYRGWNPETGTVYLKLQGACKSC 204
E++ E+ MI E+++TRIRP + +DGGDI Y G+ + G V LK+QG+C C
Sbjct: 122 --------EEDDEVVMMIKEILETRIRPMVQEDGGDITYVGF--DDGVVKLKMQGSCTGC 171
Query: 205 SSSEDTLKHGIEAMLKHYIEEVEDVVQILD 234
SS TLK+GIE ML Y+ EV++V+++ D
Sbjct: 172 PSSGVTLKNGIENMLTFYVPEVKEVIEVKD 201
>SPBC1709.19c [O] KOG2358 NifU-like domain-containing proteins
Length = 96
Score = 102 bits (255), Expect = 4e-22
Identities = 46/70 (65%), Positives = 62/70 (87%)
Query: 166 IQTRIRPAIMDDGGDIQYRGWNPETGTVYLKLQGACKSCSSSEDTLKHGIEAMLKHYIEE 225
I+T IRP+I +DGGD+++RG++ +TGTV LKL+GAC++CSSS TLK+GI+ MLKHYI E
Sbjct: 1 IETSIRPSIQEDGGDVEFRGFDEKTGTVSLKLRGACRTCSSSAVTLKNGIQQMLKHYIPE 60
Query: 226 VEDVVQILDP 235
VE+VVQ+LDP
Sbjct: 61 VENVVQVLDP 70
>At4g25910 [O] KOG2358 NifU-like domain-containing proteins
Length = 224
Score = 57.0 bits (136), Expect = 3e-08
Identities = 33/80 (41%), Positives = 47/80 (58%), Gaps = 7/80 (8%)
Query: 155 EQEISEMIDELIQTRIRPAIMDDGGDIQYRGWNPETGTVYLKLQGACKSCSSSEDTLKHG 214
E+ + ++DE +RP++M DGG++ + V LKLQGAC SC SS TLK G
Sbjct: 74 EENVERVLDE-----VRPSLMADGGNVALH--EIDGLVVVLKLQGACGSCPSSSMTLKMG 126
Query: 215 IEAMLKHYIEEVEDVVQILD 234
IE+ L+ I E+ V Q L+
Sbjct: 127 IESRLRDKIPEIMSVEQFLE 146
>At4g01940 [O] KOG2358 NifU-like domain-containing proteins
Length = 174
Score = 54.3 bits (129), Expect = 2e-07
Identities = 29/72 (40%), Positives = 45/72 (62%), Gaps = 3/72 (4%)
Query: 164 ELIQTRIRPAIMDDGGDIQYRGWNPETGTVYLKLQGACKSCSSSEDTLKHGIEAMLKH-Y 222
+L+ +RP ++ DGG++ + E G V LKLQGAC SC SS T+ GIE +LK +
Sbjct: 90 DLVLEDVRPFLISDGGNVDVV--SVEDGVVSLKLQGACTSCPSSSTTMTMGIERVLKEKF 147
Query: 223 IEEVEDVVQILD 234
+ ++D+ Q+ D
Sbjct: 148 GDALKDIRQVFD 159
>At5g49940_1 [O] KOG2358 NifU-like domain-containing proteins
Length = 215
Score = 51.6 bits (122), Expect = 1e-06
Identities = 33/88 (37%), Positives = 45/88 (50%), Gaps = 7/88 (7%)
Query: 147 PKFEYDEDEQEISEMIDELIQTRIRPAIMDDGGDIQYRGWNPETGTVYLKLQGACKSCSS 206
P E E+ + ++DE IRP +M DGG++ + V +KLQGAC SC S
Sbjct: 79 PILEVPLTEENVESVLDE-----IRPYLMSDGGNVALH--EIDGNIVRVKLQGACGSCPS 131
Query: 207 SEDTLKHGIEAMLKHYIEEVEDVVQILD 234
S T+K GIE L I E+ V + D
Sbjct: 132 STMTMKMGIERRLMEKIPEIVAVEALPD 159
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.137 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,548,050
Number of Sequences: 60738
Number of extensions: 699408
Number of successful extensions: 2038
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 2017
Number of HSP's gapped (non-prelim): 9
length of query: 256
length of database: 30,389,216
effective HSP length: 104
effective length of query: 152
effective length of database: 24,072,464
effective search space: 3659014528
effective search space used: 3659014528
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)