ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI6843 good O KOG0828 Posttranslational modification, protein turnover, chaperones Predicted E3 ubiquitin ligase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI6843 2384293 2386563 757
(757 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKL034w [O] KOG0828 Predicted E3 ubiquitin ligase 819 0.0
SPBC947.10 [O] KOG0828 Predicted E3 ubiquitin ligase 144 4e-34
At2g20650 [O] KOG0828 Predicted E3 ubiquitin ligase 127 6e-29
At1g35330 [O] KOG0800 FOG: Predicted E3 ubiquitin ligase 54 9e-07
At2g28920 [O] KOG0800 FOG: Predicted E3 ubiquitin ligase 53 2e-06
At2g44580_2 [O] KOG0800 FOG: Predicted E3 ubiquitin ligase 51 6e-06
>YKL034w [O] KOG0828 Predicted E3 ubiquitin ligase
Length = 758
Score = 819 bits (2115), Expect = 0.0
Identities = 394/751 (52%), Positives = 519/751 (68%), Gaps = 25/751 (3%)
Query: 20 SSPSGDGVTSQYEFIQLETLKSQLKYEYEVFRELSYDQNFRNITGLKLAYHDSKENPNLN 79
SSP GDGV+SQYEF QL+ LK Q + E+ F ++Y +FRNITGLKL+Y D NP N
Sbjct: 20 SSPGGDGVSSQYEFNQLQRLKQQFRTEHNTFVNMTYTDSFRNITGLKLSYQDMLNNPLQN 79
Query: 80 ATYPLPHKDYSSWKPNENYMMLPQDIIDKVQDNIWNRKQ----TLFPPNITSNLYGKINL 135
ATYPLP KDY W PN+NYM+LP D+I+ + +WN LFPPNITS L GKI+L
Sbjct: 80 ATYPLPGKDYDRWFPNQNYMVLPNDVIEAINTEVWNTSNDDASNLFPPNITSTLLGKIDL 139
Query: 136 TDNNKYTKIKMPIANFFDPPESFQDEKPADGEHYLAVDERGDAGNHGELHNVSWSYGIVD 195
NNKY KI+MP+ F++P F ++ P +GE Y + + ++GELHNVS+ +G +
Sbjct: 140 VSNNKYEKIRMPVPRFYEPATDFSEDIPPEGETYWS-----EWPSYGELHNVSFQHGEIA 194
Query: 196 VSIQHLDSVENSLSLSPASISTGESQLGEDDSHWKILHLNVNFYNREETEKHSLSTKGIY 255
+ I H+ +++++ + + + + + WK+L+L ++F ++ E EKHS+ +K +Y
Sbjct: 195 IQISHMSNLQDNNNYLRRNF------INKKNDRWKLLNLQIDFSDKAEKEKHSIYSKAVY 248
Query: 256 DIWTGRILIMSQSAKFHSLFAFPHYLA----NTEKEFNQVKRLIEKYWNATNYAESLTMA 311
DI GRIL +SQS+KFHSLFA PHY++ EK FN VK L++++WN T+Y + +TM
Sbjct: 249 DIQRGRILSISQSSKFHSLFALPHYMSFQNDYNEKIFNDVKELVDEFWNFTDYTDVMTMK 308
Query: 312 NLQHLSDEANTKCEYFGFLQLEPWDQFTPDQIRMIDEELKWPLGRPINRSNLPPIRLHNA 371
++Q + AN KCEY FLQLEPW+Q+T DQI++ID+EL WPLGRP N S+LPPI + +
Sbjct: 309 DVQDAYNNANFKCEYLIFLQLEPWNQYTRDQIKLIDDELNWPLGRPANLSSLPPINVVSG 368
Query: 372 LLYSPDCGLSLELEDVHGPRYELQVRTIRIHXXXXXXXXXXXXXXXXCQMNFTNTPSSVN 431
LLYSPDCG+ L L +V G RYEL++ +IR H QM+ TNTPS VN
Sbjct: 369 LLYSPDCGVRLGLHNVKGTRYELKIMSIRKHLLFGIALFAAQIYLLLTQMHHTNTPSMVN 428
Query: 432 KISYWCLFMMNLVDGCLAMLYFLASPLLQELYLPLCISAFACFILASVFEIRYMISVYAS 491
KIS++C M+NLVDG LA LYF+A+ ++ ELYLPL ISAF+CFILAS+FEIRY+IS+YAS
Sbjct: 429 KISFYCFSMINLVDGSLATLYFVAASVVPELYLPLVISAFSCFILASIFEIRYLISIYAS 488
Query: 492 QVNEQGVGILTLLRGGS---EANTVVNRVIPDEASISSSLYGRXXXXXXXXXXXXXXXXX 548
QVNEQ VGI+ LLRG + + N IPDE SI SLYGR
Sbjct: 489 QVNEQNVGIINLLRGNTGTYDENRPRPAFIPDEGSIGGSLYGRFFFMLIIFTFLILSSTS 548
Query: 549 WPKEIRTIFEYSVLVVLNSYWVPQICRNAVKGSDPRRRRQNGYQSLQNGDTNCIPLLWSF 608
WP+++R +FEY ++ +LNSYW+PQI RNAVKG RR R N N +PLLWSF
Sbjct: 549 WPRQLRMVFEYILIFILNSYWIPQIFRNAVKGIPSRRERARSSIG-GNRSQNKMPLLWSF 607
Query: 609 IIGTSVLRLIPIVYVFTYPSNVFRHDIDVRFAVLLSLWMLFQLLILYSQDLLGSRWFLPQ 668
+IGT+++R +P+VYVFTY SNVFRH DV F V LSLW+LFQ+ ILYSQD+LGSRWFLP+
Sbjct: 608 VIGTTIIRSLPVVYVFTYSSNVFRHHKDVHFVVFLSLWLLFQISILYSQDVLGSRWFLPK 667
Query: 669 HVIPDGYHYHRPVPQSILMEY--GSQNNCFVCPICMVDVPVYVEETEETHKIDAQSYMIT 726
H IPDGY Y +P+ + E+ G+ + C ICM DVP+Y+EE ETHK+D SYM+T
Sbjct: 668 HTIPDGYSYFKPLSNEYISEHGGGTAEHTVDCAICMSDVPIYIEEIPETHKVDQHSYMVT 727
Query: 727 PCSHIFHTECLENWMSYKLQCPVCRAPLPPL 757
PC+H+FHT CLENWM+YKLQCPVCR+PLPPL
Sbjct: 728 PCNHVFHTSCLENWMNYKLQCPVCRSPLPPL 758
>SPBC947.10 [O] KOG0828 Predicted E3 ubiquitin ligase
Length = 676
Score = 144 bits (364), Expect = 4e-34
Identities = 122/479 (25%), Positives = 198/479 (40%), Gaps = 70/479 (14%)
Query: 318 DEANTKCEYFGFLQLEPWDQFTPDQIRMIDEELKWPLGRPINRSNLPPIRLHNALLYSPD 377
+E C F FL + + ++ I+ E P G ++ +P + + + L+YSPD
Sbjct: 225 EEEEVTCSAFVFLHNKNTG-LDKETLKAIENEFYHPQG--VSTQKMPEVFV-SGLVYSPD 280
Query: 378 CGLSLELEDVHGPR-YELQVRTIRIHXXXXXXXXXXXXXXXXCQMNFTNTPSSVNKISYW 436
C ++ + GPR + L+ +R QM N+PS V ++S+
Sbjct: 281 CNVAFTFSNTKGPRNFVLENHLVRFSSLYIFIVLSQIFVLLR-QMRI-NSPSHVQRLSFL 338
Query: 437 CLFMMNLVDGCLAMLYFLASPLLQELYLPLCISAFACFILASVFEIRYMISVYASQ---- 492
+ M +D +A+ + + ++++ YLP AF + + +F +RY+ + Q
Sbjct: 339 TIAMQAGLDAYIAIFFLSTNAVIEKGYLPFVSVAFLSLVPSVMFTMRYLALILRVQNSNM 398
Query: 493 ----------------VNEQGVGILTLLRGGSEAN-----TVVNRVIPDEASIS------ 525
N+ S AN T VN D+ ++
Sbjct: 399 PPPAPRPVTNNSSNNNTNQSNASNENSPNAPSAANDNTETTTVNPPQEDDQPMTQHERDQ 458
Query: 526 ---SSLYGRXXXXXXXXXXXXXXXXXWPKEIRTIFEYSVLVVLNSYWVPQICRNAVKGSD 582
S++ R WP R F +++ S+W+PQI +N +G+
Sbjct: 459 RDWSAVCLRFYFIILVVCIASLYSAFWPVIYRFYFISALIFTSYSFWIPQIIQNVKQGTS 518
Query: 583 PRRRRQNGYQSLQNGDTNCIPLLWSFIIGTSVLRLIPIVYVFTYPSNVFRHDIDVRFAVL 642
W++I+G SVLRL + +F + FA+
Sbjct: 519 RS-------------------FTWTYILGASVLRLYLPLAIFIDSELILGFPPKYFFALG 559
Query: 643 LSLWMLFQLLILYSQDLLGSRWFLPQHVIPDG--YHYHRPVPQSILMEYGSQNNCFVCPI 700
L LWMLFQ+L+L QD LG R+FLP+ Y YH + Q L + N VCPI
Sbjct: 560 LVLWMLFQVLVLLVQDTLGPRFFLPKKFFLSSPVYDYHPVIQQDDLEAFMRDAN--VCPI 617
Query: 701 CMVDVPVYVEETEET----HKIDAQSYMITPCSHIFHTECLENWMSYKLQCPVCRAPLP 755
CM P+ + T T + ++YM+TPC H++H +CL WM + CPVCR LP
Sbjct: 618 CMQ--PIELVSTGSTLNPASMMVRRNYMLTPCHHLYHRQCLLQWMETRSICPVCRCHLP 674
>At2g20650 [O] KOG0828 Predicted E3 ubiquitin ligase
Length = 515
Score = 127 bits (319), Expect = 6e-29
Identities = 91/330 (27%), Positives = 147/330 (43%), Gaps = 88/330 (26%)
Query: 432 KISYWCLFMMNLVDGCLAMLYFLASPLLQELYLPLCISAFACFILASVFEIRYMISVYAS 491
K+S + ++D L +L+ A L++ L+ +AF F++ S+FE+RY++S++ +
Sbjct: 268 KVSIVMIGQQAIMDSYLCLLHLTAGILVESLFNAFATAAFFKFVVFSIFEMRYLLSIWKA 327
Query: 492 QVNEQGVGILTLLRGGSEANTVVNRVIPDEASISSSLYGRXXXXXXXXXXXXXXXXXWPK 551
GIL GG
Sbjct: 328 TRPSTSDGILL---GG-------------------------------------------- 340
Query: 552 EIRTIFEYS-----VLVVLNSYWVPQICRNAVKGSDPRRRRQNGYQSLQNGDTNCIPLLW 606
I ++E+ +L+++ S+W+PQI N V+ D R+ PL
Sbjct: 341 -ILLMYEFHNYMRPILLLMYSFWIPQIVANVVR--DSRK-----------------PLHP 380
Query: 607 SFIIGTSVLRLIPIVYVFTYPSNVFRHDIDVRFAVLLSLWMLFQLLILYSQDLLGSRWFL 666
+I+G +V RL +YVF P N R + + V L +M FQ +L Q GSR F+
Sbjct: 381 YYILGMTVTRLAIPLYVFGCPKNFMRVEPSKAWCVSLCAFMGFQAGVLLLQHYFGSRCFV 440
Query: 667 PQHVIPDGYHYHRPVPQSILMEYGSQNNCFVCPICMVDVPVYVEETEETHKIDAQSYMIT 726
P+ ++P+ Y Y+R + ++ N C ICM + + H+I+ M+T
Sbjct: 441 PRKLLPEKYSYYRRLDHNV-------NRSRDCVICMTTIDL-------RHRIN--DCMVT 484
Query: 727 PCSHIFHTECLENWMSYKLQCPVCRAPLPP 756
PC HIFH+ CL+ WM K++CP CR PLPP
Sbjct: 485 PCEHIFHSGCLQRWMDIKMECPTCRRPLPP 514
>At1g35330 [O] KOG0800 FOG: Predicted E3 ubiquitin ligase
Length = 327
Score = 53.9 bits (128), Expect = 9e-07
Identities = 25/59 (42%), Positives = 34/59 (57%), Gaps = 11/59 (18%)
Query: 698 CPICMVDVPVYVEETEETHKIDAQSYMITPCSHIFHTECLENWMSYKLQCPVCRAPLPP 756
C IC+ + E EET ++ + PCSH FH C++ W+S + CPVCRA LPP
Sbjct: 128 CAICLNEF-----EDEETLRL------MPPCSHAFHASCIDVWLSSRSTCPVCRASLPP 175
>At2g28920 [O] KOG0800 FOG: Predicted E3 ubiquitin ligase
Length = 145
Score = 53.1 bits (126), Expect = 2e-06
Identities = 21/57 (36%), Positives = 32/57 (55%), Gaps = 11/57 (19%)
Query: 697 VCPICMVDVPVYVEETEETHKIDAQSYMITPCSHIFHTECLENWMSYKLQCPVCRAP 753
VC IC+ D K++ ++ C H+FH +C+++W YKL CP+CRAP
Sbjct: 92 VCVICLEDF-----------KVNDVVRVLVRCKHVFHVDCIDSWCFYKLTCPICRAP 137
>At2g44580_2 [O] KOG0800 FOG: Predicted E3 ubiquitin ligase
Length = 265
Score = 51.2 bits (121), Expect = 6e-06
Identities = 23/60 (38%), Positives = 33/60 (54%), Gaps = 11/60 (18%)
Query: 698 CPICMVDVPVYVEETEETHKIDAQSYMITPCSHIFHTECLENWMSYKLQCPVCRAPLPPL 757
C IC+ D E E+ +I T CSH FH +C++ W+ K CP+CRA +PP+
Sbjct: 39 CTICLEDAA----EGEKMRRI-------TACSHCFHVDCIDPWLMKKSTCPLCRAEIPPV 87
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.137 0.427
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 46,293,758
Number of Sequences: 60738
Number of extensions: 2042658
Number of successful extensions: 5037
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 5015
Number of HSP's gapped (non-prelim): 9
length of query: 757
length of database: 30,389,216
effective HSP length: 114
effective length of query: 643
effective length of database: 23,465,084
effective search space: 15088049012
effective search space used: 15088049012
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)