ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI6950 good J KOG1627 Translation, ribosomal structure and biogenesis Translation elongation factor EF-1 gamma
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI6950 2419096 2420025 310
(310 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKL081w_2 [J] KOG1627 Translation elongation factor EF-1 gamma 310 2e-84
YPL048w_2 [J] KOG1627 Translation elongation factor EF-1 gamma 301 8e-82
SPAC29A4.02c_2 [J] KOG1627 Translation elongation factor EF-1 gamma 198 9e-51
Hs20540877_2 [J] KOG1627 Translation elongation factor EF-1 gamma 128 9e-30
Hs4503481_2 [J] KOG1627 Translation elongation factor EF-1 gamma 128 1e-29
7301765_2 [J] KOG1627 Translation elongation factor EF-1 gamma 118 1e-26
CE05656_2 [J] KOG1627 Translation elongation factor EF-1 gamma 117 2e-26
At1g57720_2 [J] KOG1627 Translation elongation factor EF-1 gamma 104 1e-22
At1g09640_2 [J] KOG1627 Translation elongation factor EF-1 gamma 104 1e-22
YPL048w_1 [O] KOG0867 Glutathione S-transferase 103 3e-22
YKL081w_1 [O] KOG0867 Glutathione S-transferase 100 5e-21
CE05656_1 [O] KOG0867 Glutathione S-transferase 69 9e-12
YGR201c [O] KOG0867 Glutathione S-transferase 62 8e-10
Hs4503481_1 [O] KOG0867 Glutathione S-transferase 60 5e-09
Hs20540877_1 [O] KOG0867 Glutathione S-transferase 56 8e-08
At1g09640_1 [O] KOG0867 Glutathione S-transferase 49 9e-06
>YKL081w_2 [J] KOG1627 Translation elongation factor EF-1 gamma
Length = 159
Score = 310 bits (794), Expect = 2e-84
Identities = 141/159 (88%), Positives = 151/159 (94%)
Query: 152 PLELLGKATFNLEEWKRTYSNEDTRPVALPWFWEHFNPEEYSIWKVGYKYNDELTLTFMS 211
PLE LGK+TF L++WKR YSN+DTRPVALPWFWEH+NPEEYSIWKVGYKYNDELTLTFMS
Sbjct: 1 PLEALGKSTFVLDDWKRKYSNDDTRPVALPWFWEHYNPEEYSIWKVGYKYNDELTLTFMS 60
Query: 212 NNLVGGFFNRLSASVKYMFGCLVVYGEXXXXGIVGAIVVRGQDFAPAFDVAPDWESYEYS 271
NNLVGGFFNRLSAS KYMFGCLVVYGE GIVGA++VRGQDFAPAFDVAPDWESYEY+
Sbjct: 61 NNLVGGFFNRLSASTKYMFGCLVVYGENNNNGIVGAVMVRGQDFAPAFDVAPDWESYEYT 120
Query: 272 KLDPTKEEDKEFINNMWAWDKPVVVNGENKEIVDGKVLK 310
KLDPTKEEDKEF+NNMWAWDKPVVVNGE+KEIVDGKVLK
Sbjct: 121 KLDPTKEEDKEFVNNMWAWDKPVVVNGEDKEIVDGKVLK 159
>YPL048w_2 [J] KOG1627 Translation elongation factor EF-1 gamma
Length = 160
Score = 301 bits (771), Expect = 8e-82
Identities = 136/160 (85%), Positives = 148/160 (92%)
Query: 151 HPLELLGKATFNLEEWKRTYSNEDTRPVALPWFWEHFNPEEYSIWKVGYKYNDELTLTFM 210
HPLELLGK+TF L++WKR YSNEDTRPVALPWFWEH+NPEEYS+WKV YKYNDELTLTFM
Sbjct: 1 HPLELLGKSTFVLDDWKRKYSNEDTRPVALPWFWEHYNPEEYSLWKVTYKYNDELTLTFM 60
Query: 211 SNNLVGGFFNRLSASVKYMFGCLVVYGEXXXXGIVGAIVVRGQDFAPAFDVAPDWESYEY 270
SNNLVGGFFNRLSAS KYMFGCLVVYGE GIVGA++VRGQD+ PAFDVAPDWESY+Y
Sbjct: 61 SNNLVGGFFNRLSASTKYMFGCLVVYGENNNNGIVGAVMVRGQDYVPAFDVAPDWESYDY 120
Query: 271 SKLDPTKEEDKEFINNMWAWDKPVVVNGENKEIVDGKVLK 310
+KLDPT ++DKEFINNMWAWDKPV VNGE KEIVDGKVLK
Sbjct: 121 AKLDPTNDDDKEFINNMWAWDKPVSVNGEPKEIVDGKVLK 160
>SPAC29A4.02c_2 [J] KOG1627 Translation elongation factor EF-1 gamma
Length = 156
Score = 198 bits (503), Expect = 9e-51
Identities = 92/160 (57%), Positives = 114/160 (70%), Gaps = 5/160 (3%)
Query: 152 PLELLGKATFNLEEWKRTYSNEDTRPVALPWFWEHFNPEEYSIWKVGYKYNDELTL-TFM 210
PL +F++EE+KR YSN+DTR ALPWF+EHF+PE YS+WKV Y Y ++L FM
Sbjct: 1 PLASAPNGSFDIEEYKRVYSNQDTRSGALPWFFEHFDPENYSVWKVDYSYPEDLKQPVFM 60
Query: 211 SNNLVGGFFNRLSASVKYMFGCLVVYGEXXXXGIVGAIVVRGQDFAPAFDVAPDWESYEY 270
+NNL+GGFF RL AS KY+FGC VV GE I GA V++G D+ PAFDVAPDW SY +
Sbjct: 61 TNNLIGGFFQRLEASRKYIFGCCVVIGENGDNTITGAFVIKGHDYVPAFDVAPDWGSYTF 120
Query: 271 SKLDPTKEEDKEFINNMWAWDKPVVVNGENKEIVDGKVLK 310
+KLD K EDK FI + WAWDKP+ E +E+ DGKV K
Sbjct: 121 TKLDINKPEDKAFIEDAWAWDKPI----EGREVADGKVCK 156
>Hs20540877_2 [J] KOG1627 Translation elongation factor EF-1 gamma
Length = 179
Score = 128 bits (322), Expect = 9e-30
Identities = 64/144 (44%), Positives = 90/144 (62%), Gaps = 6/144 (4%)
Query: 152 PLELLGKATFNLEEWKRTYSNEDTRPVALPWFWEHFNPEEYSIWKVGYKYNDELTLTFMS 211
P L K+TF L+E+KR YSNEDT VALP+FWEHF+ + +S+W Y++ +ELT TFMS
Sbjct: 21 PFAHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYSEYRFPEELTQTFMS 80
Query: 212 NNLVGGFFNRLSASVKYMFGCLVVYGEXXXXGIVGAIVVRGQDFAPAFDVAPDW----ES 267
NL+ G F RL K F ++++G I G V RGQ+ AF ++PDW ES
Sbjct: 81 CNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQEL--AFPLSPDWQVDYES 138
Query: 268 YEYSKLDPTKEEDKEFINNMWAWD 291
Y + KLDP +EE + + ++W+
Sbjct: 139 YTWRKLDPGREETQTLVREYFSWE 162
>Hs4503481_2 [J] KOG1627 Translation elongation factor EF-1 gamma
Length = 179
Score = 128 bits (321), Expect = 1e-29
Identities = 69/163 (42%), Positives = 95/163 (57%), Gaps = 8/163 (4%)
Query: 152 PLELLGKATFNLEEWKRTYSNEDTRPVALPWFWEHFNPEEYSIWKVGYKYNDELTLTFMS 211
P L K+TF L+E+KR YSNEDT VALP+FWEHF+ + +S+W Y++ +ELT TFMS
Sbjct: 21 PFAHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYSEYRFPEELTQTFMS 80
Query: 212 NNLVGGFFNRLSASVKYMFGCLVVYGEXXXXGIVGAIVVRGQDFAPAFDVAPDW----ES 267
NL+ G F RL K F ++++G I G V RGQ+ AF ++PDW ES
Sbjct: 81 CNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQEL--AFPLSPDWQVDYES 138
Query: 268 YEYSKLDPTKEEDKEFINNMWAWDKPVVVNGENKEIVDGKVLK 310
Y + KLDP EE + + ++W+ G K GK+ K
Sbjct: 139 YTWRKLDPGSEETQTLVREYFSWEGAFQHVG--KAFNQGKIFK 179
>7301765_2 [J] KOG1627 Translation elongation factor EF-1 gamma
Length = 161
Score = 118 bits (295), Expect = 1e-26
Identities = 64/163 (39%), Positives = 93/163 (56%), Gaps = 10/163 (6%)
Query: 152 PLELLGKATFNLEEWKRTYSNEDTRPVALPWFWEHFNPEEYSIWKVGYKYNDELTLTFMS 211
P + L K TFN +++KR YSNED ++P+F++ F+ E YSIW YKYN+EL+ FMS
Sbjct: 5 PFDALPKGTFNFDDFKRVYSNEDEAK-SIPYFFDKFDAENYSIWFGEYKYNEELSKVFMS 63
Query: 212 NNLVGGFFNRLSASVKYMFGCLVVYGEXXXXGIVGAIVVRGQDFAPAFDVAPDW----ES 267
NL+ G F RL K F + ++GE I G V RGQD AF ++PDW E
Sbjct: 64 CNLITGMFQRLDKMRKAAFASVCLFGEDGNSTISGIWVWRGQDL--AFTLSPDWQIDYEV 121
Query: 268 YEYSKLDPTKEEDKEFINNMWAWDKPVVVNGENKEIVDGKVLK 310
Y++ KLD EE K+ + ++W + + ++ GK+ K
Sbjct: 122 YDWKKLDAKSEETKKLVTQYFSWSG---TDKDGRKFNQGKIFK 161
>CE05656_2 [J] KOG1627 Translation elongation factor EF-1 gamma
Length = 158
Score = 117 bits (293), Expect = 2e-26
Identities = 62/163 (38%), Positives = 93/163 (57%), Gaps = 10/163 (6%)
Query: 152 PLELLGKATFNLEEWKRTYSNEDTRPVALPWFWEHFNPEEYSIWKVGYKYNDELTLTFMS 211
P + + K TF L+ +KR+YSNEDT A+P FWE+F+ + +SIWK YKY ++LTL FMS
Sbjct: 2 PFQDMPKGTFVLDNFKRSYSNEDTATKAIPHFWENFDADNWSIWKCEYKYPEDLTLAFMS 61
Query: 212 NNLVGGFFNRLSASVKYMFGCLVVYGEXXXXGIVGAIVVRGQDFAPAFDVAPDW----ES 267
NL+ G + RL K F ++++G I G V +G AF+++PDW ES
Sbjct: 62 CNLINGMYQRLEKLKKNAFASMILFGTDNNSTISGIWVWKGDKL--AFELSPDWQVDYES 119
Query: 268 YEYSKLDPTKEEDKEFINNMWAWDKPVVVNGENKEIVDGKVLK 310
Y ++KLD + K+ +N W+ + K+ GK+ K
Sbjct: 120 YTWTKLDAKSDATKKEVNEYLMWEG----DFGGKKFNQGKIFK 158
>At1g57720_2 [J] KOG1627 Translation elongation factor EF-1 gamma
Length = 159
Score = 104 bits (260), Expect = 1e-22
Identities = 60/164 (36%), Positives = 93/164 (56%), Gaps = 9/164 (5%)
Query: 151 HPLELLGKATFNLEEWKRTYSNEDT--RPVALPWFWEHFNPEEYSIWKVGYKYNDELTLT 208
+PL+LL + L++WKR YSN + R VA+ FW+ ++PE YS+W YKYNDE ++
Sbjct: 1 NPLDLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWDMYDPEGYSLWFCDYKYNDENMVS 60
Query: 209 FMSNNLVGGFFNRLSASVKYMFGCLVVYGEXXXXGIVGAIVVRGQDFAPAF--DVAPDWE 266
F++ N VGGF R+ + KY FG +++ G + G + RG + P F D D E
Sbjct: 61 FVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKGLWLFRGPEI-PKFIMDEVYDME 119
Query: 267 SYEYSKLDPTKEEDKEFINNMWAWDKPVVVNGENKEIVDGKVLK 310
YE++K+D + E KE ++ M +P E + ++D K K
Sbjct: 120 LYEWTKVDISDEAQKERVSQMIEDAEPF----EGEALLDAKCFK 159
>At1g09640_2 [J] KOG1627 Translation elongation factor EF-1 gamma
Length = 159
Score = 104 bits (260), Expect = 1e-22
Identities = 60/164 (36%), Positives = 93/164 (56%), Gaps = 9/164 (5%)
Query: 151 HPLELLGKATFNLEEWKRTYSNEDT--RPVALPWFWEHFNPEEYSIWKVGYKYNDELTLT 208
+PL+LL + L++WKR YSN + R VA+ FW+ ++PE YS+W YKYNDE ++
Sbjct: 1 NPLDLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWDMYDPEGYSLWFCDYKYNDENMVS 60
Query: 209 FMSNNLVGGFFNRLSASVKYMFGCLVVYGEXXXXGIVGAIVVRGQDFAPAF--DVAPDWE 266
F++ N VGGF R+ + KY FG +++ G + G + RG + P F D D E
Sbjct: 61 FVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKGLWLFRGPEI-PKFIMDEVYDME 119
Query: 267 SYEYSKLDPTKEEDKEFINNMWAWDKPVVVNGENKEIVDGKVLK 310
YE++K+D + E KE ++ M +P E + ++D K K
Sbjct: 120 LYEWTKVDISDEAQKERVSQMIEDAEPF----EGEALLDAKCFK 159
>YPL048w_1 [O] KOG0867 Glutathione S-transferase
Length = 255
Score = 103 bits (257), Expect = 3e-22
Identities = 48/105 (45%), Positives = 71/105 (66%)
Query: 5 VMGALLPIVGRVPYNKKSVDTSLAQLNAIGSVFEARLKNYTYLAGERITLADLFGAAILS 64
+ ++P+ G PYNKKSVD+++ ++ I +FE RLKNYTYLA E I+LADL A+I +
Sbjct: 111 IANTIVPLKGGAPYNKKSVDSAMDAVDKIVDIFENRLKNYTYLATENISLADLVAASIFT 170
Query: 65 KALSTLLDAKWRAANPATVRWFKTVVASPIISEAFGKTEFAEEAL 109
+ +L +WRA +PA VRWF TV ASP + + + +FA++ L
Sbjct: 171 RYFESLFGTEWRAQHPAIVRWFNTVRASPFLKDEYKDFKFADKPL 215
>YKL081w_1 [O] KOG0867 Glutathione S-transferase
Length = 253
Score = 99.8 bits (247), Expect = 5e-21
Identities = 46/103 (44%), Positives = 68/103 (65%)
Query: 10 LPIVGRVPYNKKSVDTSLAQLNAIGSVFEARLKNYTYLAGERITLADLFGAAILSKALST 69
L G +PYNKK VD +++ + +VF+ARL++YT++A E I+L DL A + L+T
Sbjct: 113 LSFKGLIPYNKKDVDACFVKIDNLAAVFDARLRDYTFVATENISLGDLHAAGSWAFGLAT 172
Query: 70 LLDAKWRAANPATVRWFKTVVASPIISEAFGKTEFAEEALKYT 112
+L +WRA +P +RWF TV ASPI+ F + + AE+AL YT
Sbjct: 173 ILGPEWRAKHPHLMRWFNTVAASPIVKTPFAEVKLAEKALTYT 215
>CE05656_1 [O] KOG0867 Glutathione S-transferase
Length = 240
Score = 68.9 bits (167), Expect = 9e-12
Identities = 39/108 (36%), Positives = 57/108 (52%)
Query: 4 AVMGALLPIVGRVPYNKKSVDTSLAQLNAIGSVFEARLKNYTYLAGERITLADLFGAAIL 63
AV+G +LP V ++KK+V+ +LN V + L TYL GER++LAD+ A L
Sbjct: 87 AVLGYVLPSVSAANFDKKTVEQYKNELNGQLQVLDRVLVKKTYLVGERLSLADVSVALDL 146
Query: 64 SKALSTLLDAKWRAANPATVRWFKTVVASPIISEAFGKTEFAEEALKY 111
A +LDA R + RWF+TVV P + E G+ A ++
Sbjct: 147 LPAFQYVLDANARKSIVNVTRWFRTVVNQPAVKEVLGEVSLASSVAQF 194
>YGR201c [O] KOG0867 Glutathione S-transferase
Length = 274
Score = 62.4 bits (150), Expect = 8e-10
Identities = 34/109 (31%), Positives = 49/109 (44%), Gaps = 1/109 (0%)
Query: 3 NAVMGALLPIVGRVPYNKKSVDTSLAQLNAIGSVFEARLKNYTYLA-GERITLADLFGAA 61
N V P++G PYN + ++ I S++E RLK YL + TLADL AA
Sbjct: 163 NEVCEVFFPLIGVKPYNATEFKAARENVDTIVSLYEKRLKKQQYLVCDDHETLADLISAA 222
Query: 62 ILSKALSTLLDAKWRAANPATVRWFKTVVASPIISEAFGKTEFAEEALK 110
S + D WR+ +P RWF V+ S F + E ++
Sbjct: 223 AFSLGFISFFDETWRSKHPEVTRWFNRVIKSRFFEGEFESFKMCETEMQ 271
>Hs4503481_1 [O] KOG0867 Glutathione S-transferase
Length = 258
Score = 59.7 bits (143), Expect = 5e-09
Identities = 30/103 (29%), Positives = 54/103 (52%)
Query: 9 LLPIVGRVPYNKKSVDTSLAQLNAIGSVFEARLKNYTYLAGERITLADLFGAAILSKALS 68
+ P +G + +NK++ + + ++ I + +A LK T+L GER+TLAD+ L
Sbjct: 114 VFPTLGIMHHNKQATENAKEEVRRILGLLDAYLKTRTFLVGERVTLADITVVCTLLWLYK 173
Query: 69 TLLDAKWRAANPATVRWFKTVVASPIISEAFGKTEFAEEALKY 111
+L+ +R A P T RWF T + P G+ + E+ ++
Sbjct: 174 QVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQF 216
>Hs20540877_1 [O] KOG0867 Glutathione S-transferase
Length = 258
Score = 55.8 bits (133), Expect = 8e-08
Identities = 29/103 (28%), Positives = 53/103 (51%)
Query: 9 LLPIVGRVPYNKKSVDTSLAQLNAIGSVFEARLKNYTYLAGERITLADLFGAAILSKALS 68
+ P +G + +NK++ + + ++ I + +A LK T+L GER+TLAD+ L
Sbjct: 114 VFPTLGIMHHNKQATENAKEEVRRILGLLDAYLKTRTFLVGERVTLADITVVCTLLWLYK 173
Query: 69 TLLDAKWRAANPATVRWFKTVVASPIISEAFGKTEFAEEALKY 111
+L+ +R A T RWF T + P G+ + E+ ++
Sbjct: 174 QVLEPSFRRAFRNTNRWFLTCINQPQFRAVLGELKLCEKMAQF 216
>At1g09640_1 [O] KOG0867 Glutathione S-transferase
Length = 255
Score = 48.9 bits (115), Expect = 9e-06
Identities = 27/96 (28%), Positives = 47/96 (48%)
Query: 11 PIVGRVPYNKKSVDTSLAQLNAIGSVFEARLKNYTYLAGERITLADLFGAAILSKALSTL 70
P +G +PY+ + + +++ L L + TYL G ITLAD+ L+ +T+
Sbjct: 115 PRMGFMPYSAPAEEGAISTLKRALDALNTHLTSNTYLVGHSITLADIITVCNLNLGFATV 174
Query: 71 LDAKWRAANPATVRWFKTVVASPIISEAFGKTEFAE 106
+ K+ + P R+F TVV P ++ G + E
Sbjct: 175 MTKKFTSEFPHVERYFWTVVNQPNFTKVLGDVKQTE 210
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.136 0.415
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,474,363
Number of Sequences: 60738
Number of extensions: 623414
Number of successful extensions: 1302
Number of sequences better than 1.0e-05: 16
Number of HSP's better than 0.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1276
Number of HSP's gapped (non-prelim): 16
length of query: 310
length of database: 30,389,216
effective HSP length: 106
effective length of query: 204
effective length of database: 23,950,988
effective search space: 4886001552
effective search space used: 4886001552
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)