ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI7025 check: MH U KOG2058 Intracellular trafficking, secretion, and vesicular transport Ypt/Rab GTPase activating protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI7025 2447842 2448750 303
(303 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YMR055c [U] KOG2058 Ypt/Rab GTPase activating protein 377 e-104
SPAC6F6.08c [U] KOG2058 Ypt/Rab GTPase activating protein 213 2e-55
SPBC215.01 [R] KOG4347 GTPase-activating protein VRP 71 2e-12
YGR100w [R] KOG4347 GTPase-activating protein VRP 59 9e-09
CE02529 [R] KOG1093 Predicted protein kinase (contains TBC and R... 59 9e-09
CE10236 [TR] KOG2222 Uncharacterized conserved protein contains ... 57 3e-08
Hs5902154 [R] KOG4347 GTPase-activating protein VRP 53 5e-07
Hs22042713 [R] KOG4347 GTPase-activating protein VRP 52 1e-06
Hs17485595 [R] KOG4347 GTPase-activating protein VRP 51 2e-06
Hs11034851 [TR] KOG2222 Uncharacterized conserved protein contai... 50 4e-06
7295061 [R] KOG4567 GTPase-activating protein 49 7e-06
>YMR055c [U] KOG2058 Ypt/Rab GTPase activating protein
Length = 306
Score = 377 bits (968), Expect = e-104
Identities = 192/301 (63%), Positives = 228/301 (74%), Gaps = 5/301 (1%)
Query: 2 SIEKFISQPPLIVHSSLSQLRYLVLSEGL---SDEDHQRTRSHVWSILSRASLEKCTQEY 58
SIE IS PPL++HSSLSQLRYL+LSEGL D+ QRTR +VW++LS+ S+E TQ Y
Sbjct: 3 SIEDLISNPPLLLHSSLSQLRYLILSEGLPISEDKQQQRTRCYVWTVLSQTSMEASTQRY 62
Query: 59 LRLVQLGAPPSNWISKIKNDTFRTLSTDEKFRNKVTESQLIRVLSCFAWDVLDSNQTHEL 118
L L++LG P + KIKNDT RT TD FRN+V+E LIR LSCFAW Q
Sbjct: 63 LALLKLGPPSTTIYQKIKNDTSRTFQTDPNFRNRVSEDALIRCLSCFAWQTQQRRQKTRF 122
Query: 119 PGL--SPYVQGMNVLLAPFLYVCPTEPIAYKLFHSLCFQMIPTYLNQSLSGVHVAAKLLD 176
+ S YVQGMNVLLAP LY CP+EP+AY+LF LC++MIPTYL ++L+G AKLLD
Sbjct: 123 GRIPVSTYVQGMNVLLAPLLYSCPSEPMAYQLFTKLCYEMIPTYLTKNLNGAQNGAKLLD 182
Query: 177 KCLKIIDPKLSKFLTDNLLTAEIFGIPSILTLSACNKPLDQVIKLWDFMFAYGFHMNILF 236
L+IIDPKLSKFL+DNLLTAEI+G+PSILTLS+CNKPLDQVIKLWDFMFAYGFHMNILF
Sbjct: 183 ISLRIIDPKLSKFLSDNLLTAEIYGMPSILTLSSCNKPLDQVIKLWDFMFAYGFHMNILF 242
Query: 237 IVAQLVINRQAIMVSSSPMNLLRETPSINADEIITLGVGFLAKLPDTLYTNLVDHLTDES 296
+VA LV R + S SP+NLLR+ P +ADEII LGVGF+AK+P +Y LVDHLTD
Sbjct: 243 VVAFLVKMRSKVFKSDSPVNLLRQFPDFDADEIIRLGVGFIAKIPAQIYDLLVDHLTDPD 302
Query: 297 I 297
I
Sbjct: 303 I 303
>SPAC6F6.08c [U] KOG2058 Ypt/Rab GTPase activating protein
Length = 299
Score = 213 bits (543), Expect = 2e-55
Identities = 115/294 (39%), Positives = 176/294 (59%), Gaps = 16/294 (5%)
Query: 3 IEKFISQPPLIVHSSLSQLRYLVLSEGLSDED--HQRTRSHVWSILSRASLEKCTQEYLR 60
++K + P +S+LRY+V+ + + ++ + TR +VW++L A + EY+R
Sbjct: 9 LQKLAPKSPENQSKCISRLRYMVMLDQVESDEGGNSSTRPYVWAVLLNAP-PRNADEYIR 67
Query: 61 LVQLGAPPSNWISKIKNDTFRTLSTDEKFRNKVTESQLIRVLSCFAWDVLDSNQTHELPG 120
V+ G PS KI+ND RTL + +F ++V++S L R+L+ + W
Sbjct: 68 YVRQG--PSPMAQKIQNDVSRTLVVESQFHSRVSQSSLSRLLNAYVWK-----------R 114
Query: 121 LSPYVQGMNVLLAPFLYVCPTEPIAYKLFHSLCFQMIPTYLNQSLSGVHVAAKLLDKCLK 180
+ YVQGMNVL +PFLY C +E A++ F L P Y+ ++ GVH AKLLDKCL+
Sbjct: 115 GALYVQGMNVLASPFLYACKSENQAFQFFDRLLQNECPLYVLPNIDGVHRGAKLLDKCLE 174
Query: 181 IIDPKLSKFLTDNLLTAEIFGIPSILTLSACNKPLDQVIKLWDFMFAYGFHMNILFIVAQ 240
++D +L +L LTA+I+ +PSILTLSAC PL + + +WDF+FAYG H+NIL ++AQ
Sbjct: 175 VLDHRLYTYLLSKGLTAKIYALPSILTLSACTAPLSEALTIWDFLFAYGIHLNILCVIAQ 234
Query: 241 LVINRQAIMVSSSPMNLLRETPSINADEIITLGVGFLAKLPDTLYTNLVDHLTD 294
+ I R+ ++ SPM LLR P +NA I+ + + ++KLP LY L H D
Sbjct: 235 MFIFREQLIDHPSPMTLLRTFPPLNAKNIMKITILLISKLPPELYNLLARHAWD 288
>SPBC215.01 [R] KOG4347 GTPase-activating protein VRP
Length = 827
Score = 70.9 bits (172), Expect = 2e-12
Identities = 60/250 (24%), Positives = 112/250 (44%), Gaps = 14/250 (5%)
Query: 12 LIVHSSLSQLRYLVLSEGLSDEDHQRTRSHVWSILSRASLEKC--TQEYLRLVQLGAPPS 69
L+ ++LS +R + S+ + E + R +W + S + + + EY L+++ + +
Sbjct: 199 LLYGANLSLIRVSLFSKLVRIELPNKLRGEIWELTSGSMYFRLENSDEYDHLLKVYSGQT 258
Query: 70 NW-ISKIKNDTFRTLSTDEKFRNKVTESQLIRVLSCFAWDVLDSNQTHELPGLSPYVQGM 128
++ + +I+ D R+L ++N+ + L VL F+W NQ Y Q M
Sbjct: 259 SFSLEEIEKDLGRSLPEYPAYQNEEGINALRNVLVAFSW----KNQE------VGYCQAM 308
Query: 129 NVLLAPFLYVCPTEPIAYKLFHSLCFQMIPTYLNQSLSGVHVAAKLLDKCLKIIDPKLSK 188
N++ A L C TE + L H +C IP Y ++++ G + ++ + ++ P L
Sbjct: 309 NIVAAALLIHC-TEEQTFFLMHKICEDYIPGYYSKTMYGTLIDQQVYESLVQRSMPNLHA 367
Query: 189 FLTDNLLTAEIFGIPSILTLSACNKPLDQVIKLWDFMFAYGFHMNILFIVAQLVINRQAI 248
+ I +P L+L C PL +L DF F G + +A L N I
Sbjct: 368 HFVSKDIQLSIISLPWFLSLFLCTMPLPYAFRLLDFFFLEGPRVLFQIGMAILYDNEAEI 427
Query: 249 MVSSSPMNLL 258
M ++ L+
Sbjct: 428 MKATEDTMLI 437
>YGR100w [R] KOG4347 GTPase-activating protein VRP
Length = 950
Score = 58.9 bits (141), Expect = 9e-09
Identities = 49/216 (22%), Positives = 97/216 (44%), Gaps = 14/216 (6%)
Query: 37 RTRSHVWSILSRASLEKCTQ--EYLRLVQLGA-PPSNWISKIKNDTFRTLSTDEKFRNKV 93
R R +W + S A + EY R++ A S I +I+ D R+L ++ +
Sbjct: 248 RMRGEIWELCSGAMYMRYANSGEYERILNENAGKTSQAIDEIEKDLKRSLPEYSAYQTEE 307
Query: 94 TESQLIRVLSCFAWDVLDSNQTHELPGLSPYVQGMNVLLAPFLYVCPTEPIAYKLFHSLC 153
+L VL+ ++W D Y Q MN+++A FL + +E A+ +LC
Sbjct: 308 GIQRLRNVLTAYSWKNPDVG----------YCQAMNIVVAGFL-IFMSEEQAFWCLCNLC 356
Query: 154 FQMIPTYLNQSLSGVHVAAKLLDKCLKIIDPKLSKFLTDNLLTAEIFGIPSILTLSACNK 213
+P Y ++++ G + ++ + ++ P L +++ + + + +P L+L +
Sbjct: 357 DIYVPGYYSKTMYGTLLDQRVFESFVEDRMPVLWEYILQHDIQLSVVSLPWFLSLFFTSM 416
Query: 214 PLDQVIKLWDFMFAYGFHMNILFIVAQLVINRQAIM 249
PL+ +++ D F G +A L IN I+
Sbjct: 417 PLEYAVRIMDIFFMNGSITLFQVALAVLKINADDIL 452
>CE02529 [R] KOG1093 Predicted protein kinase (contains TBC and RHOD
domains)
Length = 826
Score = 58.9 bits (141), Expect = 9e-09
Identities = 60/247 (24%), Positives = 104/247 (41%), Gaps = 15/247 (6%)
Query: 39 RSHVWSILSRASLEKCTQEYLRLVQLGAPPSNWISKIKNDTFRTLSTDEKFRNKVTESQL 98
R+ VW L + K ++YL L L + S+ +++ D R D + L
Sbjct: 453 RADVWCTLLDINETKW-RDYLNLDVLASHSSD--RQLEVDIPRCHQYDSYMTTPAIQESL 509
Query: 99 IRVLSCFAWDVLDSNQTHELPGLSPYVQGMNVLLAPFLYVCPTEP-IAYKLFHSLCFQMI 157
+VL W ++ +Q Y QG + L PFL ++P +A+ F ++
Sbjct: 510 RKVLK--GWQIVTESQHFV------YWQGCDSLATPFLLANMSKPHVAFACFKEFTYRYC 561
Query: 158 PT-YLNQSLSGVHVAAKLLDKCLKIIDPKLSKFLTDNLLTAEIFGIPSILTLSACNKPLD 216
YL + + + + DP L K L N AE+F IP LT A PL
Sbjct: 562 HKFYLKDNSEVIKEYLGIFYHLVAYTDPVLYKHLKINGFDAELFAIPWFLTCFAHELPLS 621
Query: 217 QVIKLWDFMFAYGFHMNILFIVAQLVINRQAIMV--SSSPMNLLRETPSINADEIITLGV 274
+++ LWD +G ++ +A L R ++ + + ++ + P ++ DEII
Sbjct: 622 KLVLLWDETMMHGNAFPLMIALAMLNRLRDKLLAVNFNEMIIIIHDQPDLSIDEIIKNSR 681
Query: 275 GFLAKLP 281
G+ +P
Sbjct: 682 GYEKLIP 688
>CE10236 [TR] KOG2222 Uncharacterized conserved protein contains TBC SH3 and
RUN domains
Length = 808
Score = 57.0 bits (136), Expect = 3e-08
Identities = 65/272 (23%), Positives = 119/272 (42%), Gaps = 29/272 (10%)
Query: 39 RSHVWSILSRASLEKCTQEYLR---LVQLGAPPSNWISKIKNDTFRTLSTDEKFRNKVTE 95
R +W LS A+ ++ +Y L Q + +I+ D RTL + F K +E
Sbjct: 156 RPFLWPRLSGATKKQKDAKYTYESVLQQCAQDKPSIGVQIERDLLRTLPNNICFWKKNSE 215
Query: 96 S--QLIRVLSCFAWDVLDSNQTHELPGLSPYVQGMNVLLAPFLYVCPTEPIAYKLFHSLC 153
L R+L C A+ D Y QGM V++A L C E + + +
Sbjct: 216 GIEALRRILKCVAFIYPDLG----------YCQGMGVIVATLLLYCSEETTFWMMTALIE 265
Query: 154 FQMIPTYLNQSLSGVHVAAKLLDKCLKIIDPKLSKFLTDNLLTAEIFGIPSILTLSACNK 213
+ P + Q+L G+ ++ +K P L+K L D + + + +LTL
Sbjct: 266 DILPPNFYTQTLLGLQADERVSRHLMKCHVPDLNKALEDYEVEVSLLTVSWLLTLFGSVF 325
Query: 214 PLDQVIKLWDFMFAYGFHMNILFIVAQLVINRQAIMVSSSPMNLLRETPSINADEIITLG 273
++++WDF+F Y +NI ++ ++ ++ +V + ET +AD I T
Sbjct: 326 RTRVMLRVWDFIF-YSGGVNIFRVIISILKMKEQEIVE------IAETTQSSAD-IFTA- 376
Query: 274 VGFLAKLPD--TLYTNLVDHLTDESITMSNHL 303
L++LP T +++++ T+++HL
Sbjct: 377 ---LSQLPASVTEVEKVIEYMGSFEFTITDHL 405
>Hs5902154 [R] KOG4347 GTPase-activating protein VRP
Length = 897
Score = 53.1 bits (126), Expect = 5e-07
Identities = 46/178 (25%), Positives = 79/178 (43%), Gaps = 12/178 (6%)
Query: 74 KIKNDTFRTLSTDEKFRNKVTESQLIRVLSCFAWDVLDSNQTHELPGLSPYVQGMNVLLA 133
+I+ D R+L F+N+ + L RVL+ +A H P + Y Q MN+L +
Sbjct: 306 EIERDLHRSLPEHPAFQNETGIAALRRVLTAYA---------HRNPKIG-YCQSMNILTS 355
Query: 134 PFLYVCPTEPIAYKLFHSLCFQMIPTYLNQSLSGVHVAAKLLDKCLKIIDPKLSKFLTDN 193
L E A+ L ++C +M+P Y N + G V + ++ +K P+L++ + D
Sbjct: 356 VLLLYTKEEE-AFWLLVAVCERMLPDYFNHRVIGAQVDQSVFEELIKGHLPELAEHMNDL 414
Query: 194 LLTAEIFGIPSILTLSACNKPLDQVIKLWDFMFAYGFHMNILFIVAQLVINRQAIMVS 251
A + + LTL PL+ + + D F G +A L N + + S
Sbjct: 415 SALASV-SLSWFLTLFLSIMPLESAVNVVDCFFYDGIKAIFQLGLAVLEANAEDLCSS 471
>Hs22042713 [R] KOG4347 GTPase-activating protein VRP
Length = 1266
Score = 52.0 bits (123), Expect = 1e-06
Identities = 54/209 (25%), Positives = 93/209 (43%), Gaps = 17/209 (8%)
Query: 39 RSHVWSILSRASLEKCTQE--YLRLVQLGAPPSNWISK-IKNDTFRTLSTDEKFRNKVTE 95
R +W +LS A EK T Y LV+ N ++ I+ D R+L F+N++
Sbjct: 521 RGELWLLLSGAINEKATHPGYYEDLVEKSMGKYNLATEEIERDLHRSLPEHPAFQNEMGI 580
Query: 96 SQLIRVLSCFAWDVLDSNQTHELPGLSPYVQGMNVLLAPFLYVCPTEPIAYKLFHSLCFQ 155
+ L RVL+ +A+ P + Y Q MN++ + L E A+ L +LC +
Sbjct: 581 AALRRVLTAYAF---------RNPNIG-YCQAMNIVTSVLLLYAKEEE-AFWLLVALCER 629
Query: 156 MIPTYLNQSLSGVHVAAKLLDKCLKIIDPKLSKFLTDNLLTAEIFGIPSILTLSACNKPL 215
M+P Y N + G V + ++ + P+L + D L + LTL P
Sbjct: 630 MLPDYYNTRVVGALVDQGVFEELARDYVPQLYDCMQD-LGVISTISLSWFLTLFLSVMPF 688
Query: 216 DQVIKLWDFMFAYGFHMNILFIVAQLVIN 244
+ + + D F G + ++F +A V++
Sbjct: 689 ESAVVVVDCFFYEG--IKVIFQLALAVLD 715
>Hs17485595 [R] KOG4347 GTPase-activating protein VRP
Length = 796
Score = 50.8 bits (120), Expect = 2e-06
Identities = 47/194 (24%), Positives = 87/194 (44%), Gaps = 15/194 (7%)
Query: 39 RSHVWSILSRASLEKCTQE--YLRLVQLGAPPSNWISK-IKNDTFRTLSTDEKFRNKVTE 95
R +W + S A + T Y +V+ N ++ I+ D R+L F++
Sbjct: 493 RGELWMLFSGAVNDMATNPDYYTEVVEQSLGTCNLATEEIERDLRRSLPEHPAFQSDTGI 552
Query: 96 SQLIRVLSCFAWDVLDSNQTHELPGLSPYVQGMNVLLAPFLYVCPTEPIAYKLFHSLCFQ 155
S L RVL+ +A+ P + Y Q MN+L + L E A+ L ++C +
Sbjct: 553 SALRRVLTAYAY---------RNPKIG-YCQAMNILTSVLLLYAKEEE-AFWLLVAVCER 601
Query: 156 MIPTYLNQSLSGVHVAAKLLDKCLKIIDPKLSKFLTDNLLTAEIFGIPSILTLSACNKPL 215
M+P Y N+ + G V + ++ ++ P+L++ +TD + + + LTL P+
Sbjct: 602 MLPDYFNRRIIGALVDQAVFEELIRDHLPQLTEHMTDMTFFSSV-SLSWFLTLFISVLPI 660
Query: 216 DQVIKLWDFMFAYG 229
+ + + D F G
Sbjct: 661 ESAVNVVDCFFYDG 674
>Hs11034851 [TR] KOG2222 Uncharacterized conserved protein contains TBC SH3 and
RUN domains
Length = 777
Score = 50.1 bits (118), Expect = 4e-06
Identities = 56/245 (22%), Positives = 104/245 (41%), Gaps = 20/245 (8%)
Query: 39 RSHVWSILSRASLEKCTQE--YLRLVQLGAPPSNWISK-IKNDTFRTLSTDEKFRN--KV 93
R +W LS A +K E Y +V+ + +K I+ D RT+ ++ F + +
Sbjct: 120 RPQLWMRLSGALQKKRNSELSYREIVKNSSNDETIAAKQIEKDLLRTMPSNACFASMGSI 179
Query: 94 TESQLIRVLSCFAWDVLDSNQTHELPGLSPYVQGMNVLLAPFLYVCPTEPIAYKLFHSLC 153
+L RVL AW + Y QG ++ A L E A+ + ++
Sbjct: 180 GVPRLRRVLRALAWLYPEIG----------YCQGTGMVAACLLLFLEEED-AFWMMSAII 228
Query: 154 FQMIP-TYLNQSLSGVHVAAKLLDKCLKIIDPKLSKFLTDNLLTAEIFGIPSILTLSACN 212
++P +Y + +L GV ++L + P+L K L ++ + + + LT A
Sbjct: 229 EDLLPASYFSTTLLGVQTDQRVLRHLIVQYLPRLDKLLQEHDIELSLITLHWFLTAFASV 288
Query: 213 KPLDQVIKLWDFMFAYGFHMNILFIVAQLVINRQAIMV---SSSPMNLLRETPSINADEI 269
+ ++++WD F G + + L + + ++ S+S N L + PS D
Sbjct: 289 VDIKLLLRIWDLFFYEGSRVLFQLTLGMLHLKEEELIQSENSASIFNTLSDIPSQMEDAE 348
Query: 270 ITLGV 274
+ LGV
Sbjct: 349 LLLGV 353
>7295061 [R] KOG4567 GTPase-activating protein
Length = 403
Score = 49.3 bits (116), Expect = 7e-06
Identities = 41/164 (25%), Positives = 73/164 (44%), Gaps = 17/164 (10%)
Query: 124 YVQGMNVLLAPFLYVCPTEPIAYKLFHS-----LCFQMIPT--------YLNQSLSGVHV 170
YVQGMN ++ P YV ++P H+ CF + + L+ + G+
Sbjct: 233 YVQGMNEIVGPIYYVMASDPDLTYRAHAEADCFFCFTALMSEIRDFFIKTLDDAEGGIKF 292
Query: 171 AAKLLDKCLKIIDPKLSKFLTDNLLTAEIFGIPSILTLSACNKPLDQVIKLWDFMFA--Y 228
L LK D + + L L + + + L + PL V+++WD +FA
Sbjct: 293 MMARLSNMLKSKDLSIYELLRSQELHPQYYSFRWLTLLLSQEFPLPDVLRIWDSVFADEQ 352
Query: 229 GFHMNILFIVAQLVINRQAIMVS--SSPMNLLRETPSINADEII 270
F I + ++I R+AI+ + +S + LL+ P I+ + +I
Sbjct: 353 RFDFLIKICCSMILIQREAILENDFASNVKLLQNYPPIDINVVI 396
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.323 0.137 0.405
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,786,353
Number of Sequences: 60738
Number of extensions: 717966
Number of successful extensions: 1806
Number of sequences better than 1.0e-05: 11
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 1797
Number of HSP's gapped (non-prelim): 11
length of query: 303
length of database: 30,389,216
effective HSP length: 106
effective length of query: 197
effective length of database: 23,950,988
effective search space: 4718344636
effective search space used: 4718344636
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)