ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI7028 good A KOG0120 RNA processing and modification Splicing factor U2AF, large subunit (RRM superfamily)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI7028 2450517 2448751 -589
(589 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKL074c [A] KOG0120 Splicing factor U2AF large subunit (RRM supe... 143 6e-34
At1g60900 [A] KOG0120 Splicing factor U2AF large subunit (RRM su... 55 2e-07
At1g60830 [A] KOG0120 Splicing factor U2AF large subunit (RRM su... 54 9e-07
7291545 [A] KOG0120 Splicing factor U2AF large subunit (RRM supe... 54 9e-07
7293214 [A] KOG0120 Splicing factor U2AF large subunit (RRM supe... 53 1e-06
CE29149 [A] KOG0120 Splicing factor U2AF large subunit (RRM supe... 52 3e-06
CE27339 [A] KOG0120 Splicing factor U2AF large subunit (RRM supe... 52 3e-06
CE25626 [A] KOG0120 Splicing factor U2AF large subunit (RRM supe... 52 3e-06
>YKL074c [A] KOG0120 Splicing factor U2AF large subunit (RRM superfamily)
Length = 527
Score = 143 bits (361), Expect = 6e-34
Identities = 122/483 (25%), Positives = 222/483 (45%), Gaps = 44/483 (9%)
Query: 131 KSPRQDNRERQSRFQRNDSSISKPAFNSDNSFQQNRGHEVRNGRFDNKNRWSDASNASHS 190
K+P+ N + R SSI + N +++Q R R D R + N S
Sbjct: 55 KAPKSRNVSHSNN--RGPSSIITMSTNR-TTYEQTRAGPHRQSYRDASGRSYNRENRYSS 111
Query: 191 RNTNLQVKNN----QADDSEGHNFRKPTEWKKDLSHNTRGRFE-PNKNRFGHAQAERYRT 245
NT Q NN Q D+ G N R + + R ++ N+ + ++ +
Sbjct: 112 HNTGPQWNNNPYNRQRDERRGRNERFDRRGRNGNGNYDRFNYQRKNEGSKFNGDRDKRQL 171
Query: 246 KTPNPSASWSSEDAVINGNATGRSVIPPYETILKNVTNVRLNRTLIVTPMIPGFEEHVSV 305
+T +++S++ + G++ P Y N+ + + N L+++ + + S+
Sbjct: 172 QTNKYDMNYNSQNVMYPGSSFDS---PAYY----NMASSKANSRLVISGLSQSSDP--SI 222
Query: 306 VAIIKEFIGDVDCDLDTKN------EVTSSIIEKNR---IVMEFNKESLVTIVLTCQKYL 356
VA +K+ + + L ++++ I + R I++EF+ + T+VL C+ +
Sbjct: 223 VARLKDLLENFISGLQKTESNAEDFKISNFYIGEGRPDHIIVEFSSQICSTMVLACRSFF 282
Query: 357 ESKISS-QFIWSRPNGCVNDTGNVQPINHGSVLSLLKLGPECEDEESTKQWLID------ 409
+K+ + W RPN V ++ G+V++L L E E+ + L
Sbjct: 283 NAKLGTFDLKWRRPNDYVQQLDHLVDFCRGTVIALENLENIGEGEDYRMKELFSSLNVTN 342
Query: 410 ---EQVEYSWLQLMKLKGSDERTSWIKALLYEPKAED---QKKLPMTIRPNQSSMQQNFK 463
+ + Y G + + L +E +D + K +PN + Q
Sbjct: 343 GTAKPLFYKCSSNTNNTGKESEFTKCILLSFEVVTQDILDKLKPYKWFKPNDGKISQVTS 402
Query: 464 SITYENFPHLVKKYEKKPSKVVCVLNSVDPMDLKNEKFVTEIKEAMMYGTPIQSCGEVES 523
IT+++ P+LV + + S+V+ +LN +DP+DLK+E F+TEIKE + Y S ++
Sbjct: 403 WITFQSLPNLVTQSVRVESRVLLLLNCLDPLDLKDETFITEIKETLKY-----SIAGADT 457
Query: 524 VQIPLPNPDYRNDMSHIHSSIGKVFIKFRDLVGAEKAMLVLPGSQFCQRTILCSYYSETD 583
++I P DYR + ++ S G ++IKF+ L A+ AM LPG+QF RT+LC+Y E D
Sbjct: 458 IKICQPGVDYRLNFENLASGAGNIYIKFKTLEAAKHAMEELPGTQFNDRTVLCTYIDEDD 517
Query: 584 YDM 586
+DM
Sbjct: 518 FDM 520
>At1g60900 [A] KOG0120 Splicing factor U2AF large subunit (RRM superfamily)
Length = 568
Score = 55.5 bits (132), Expect = 2e-07
Identities = 34/107 (31%), Positives = 54/107 (49%), Gaps = 7/107 (6%)
Query: 481 PSKVVCVLNSVDPMDLKNEKFVTEIKEAMMYGTPIQSCGEVESVQIPLPNPDYRNDMSHI 540
P+K+VC+ V DL++++ EI E M G + +V IP PNPD+
Sbjct: 465 PTKIVCLTQVVTADDLRDDEEYAEIMEDMRQEGG--KFGNLVNVVIPRPNPDHDPT---- 518
Query: 541 HSSIGKVFIKFRDLVGAEKAMLVLPGSQFCQRTILCSYYSETDYDMG 587
+GKVF+++ D+ G+ KA + G +F ++ YY E Y G
Sbjct: 519 -PGVGKVFLEYADVDGSSKARSGMNGRKFGGNQVVAVYYPEDKYAQG 564
>At1g60830 [A] KOG0120 Splicing factor U2AF large subunit (RRM superfamily)
Length = 111
Score = 53.5 bits (127), Expect = 9e-07
Identities = 33/107 (30%), Positives = 53/107 (48%), Gaps = 7/107 (6%)
Query: 481 PSKVVCVLNSVDPMDLKNEKFVTEIKEAMMYGTPIQSCGEVESVQIPLPNPDYRNDMSHI 540
P+K+VC+ V DL+++ +I E M G + +V IP PNPD+
Sbjct: 8 PTKIVCLTQVVTADDLRDDAEYADIMEDMSQEGG--KFGNLVNVVIPRPNPDHDPT---- 61
Query: 541 HSSIGKVFIKFRDLVGAEKAMLVLPGSQFCQRTILCSYYSETDYDMG 587
+GKVF+++ D+ G+ KA + G +F ++ YY E Y G
Sbjct: 62 -PGVGKVFLEYADVDGSSKARSGMNGRKFGGNQVVAVYYPEDKYAQG 107
>7291545 [A] KOG0120 Splicing factor U2AF large subunit (RRM superfamily)
Length = 449
Score = 53.5 bits (127), Expect = 9e-07
Identities = 32/107 (29%), Positives = 55/107 (50%), Gaps = 14/107 (13%)
Query: 481 PSKVVCVLNSVDPMDLKNEKFVTEIKEAMMYGTPIQSC---GEVESVQIPLPNPDYRNDM 537
P++ +C+LN V P +L +++ +I+ + Q C GEV S++IP P +
Sbjct: 348 PTETLCLLNMVRPEELLDDEEFEDIRTDIK-----QECAKFGEVRSIKIPRPIGQFPK-- 400
Query: 538 SHIHSSIGKVFIKFRDLVGAEKAMLVLPGSQFCQRTILCSYYSETDY 584
GKVF++F + ++KA+ L G +F R ++ SYY Y
Sbjct: 401 ----RGCGKVFVQFESVEDSQKALKALSGRKFSGRIVMTSYYDPEKY 443
>7293214 [A] KOG0120 Splicing factor U2AF large subunit (RRM superfamily)
Length = 416
Score = 52.8 bits (125), Expect = 1e-06
Identities = 33/107 (30%), Positives = 54/107 (49%), Gaps = 14/107 (13%)
Query: 481 PSKVVCVLNSVDPMDLKNEKFVTEIKEAMMYGTPIQSC---GEVESVQIPLPNPDYRNDM 537
P++V+C+LN V P +L++E+ +I E + + C G V SV+IP P
Sbjct: 316 PTEVLCLLNMVTPDELRDEEEYEDILEDIK-----EECTKYGVVRSVEIPRPIEGVEVP- 369
Query: 538 SHIHSSIGKVFIKFRDLVGAEKAMLVLPGSQFCQRTILCSYYSETDY 584
GKVF++F ++ +KA L G +F R ++ SY+ Y
Sbjct: 370 -----GCGKVFVEFNSVLDCQKAQQALTGRKFSDRVVVTSYFDPDKY 411
>CE29149 [A] KOG0120 Splicing factor U2AF large subunit (RRM superfamily)
Length = 474
Score = 51.6 bits (122), Expect = 3e-06
Identities = 31/105 (29%), Positives = 51/105 (48%), Gaps = 8/105 (7%)
Query: 480 KPSKVVCVLNSVDPMDLKNEKFVTEIKEAMMYGTPIQSCGEVESVQIPLPNPDYRNDMSH 539
+ ++++C++N V +LK + EI E + G V S++IP P D H
Sbjct: 373 RATEILCLMNMVTEDELKADDEYEEILEDVR--DECSKYGIVRSLEIPRPYED------H 424
Query: 540 IHSSIGKVFIKFRDLVGAEKAMLVLPGSQFCQRTILCSYYSETDY 584
+GKVF++F ++A L G +F RT++ SYY Y
Sbjct: 425 PVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKY 469
>CE27339 [A] KOG0120 Splicing factor U2AF large subunit (RRM superfamily)
Length = 496
Score = 51.6 bits (122), Expect = 3e-06
Identities = 31/105 (29%), Positives = 51/105 (48%), Gaps = 8/105 (7%)
Query: 480 KPSKVVCVLNSVDPMDLKNEKFVTEIKEAMMYGTPIQSCGEVESVQIPLPNPDYRNDMSH 539
+ ++++C++N V +LK + EI E + G V S++IP P D H
Sbjct: 395 RATEILCLMNMVTEDELKADDEYEEILEDVR--DECSKYGIVRSLEIPRPYED------H 446
Query: 540 IHSSIGKVFIKFRDLVGAEKAMLVLPGSQFCQRTILCSYYSETDY 584
+GKVF++F ++A L G +F RT++ SYY Y
Sbjct: 447 PVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKY 491
>CE25626 [A] KOG0120 Splicing factor U2AF large subunit (RRM superfamily)
Length = 143
Score = 51.6 bits (122), Expect = 3e-06
Identities = 31/105 (29%), Positives = 51/105 (48%), Gaps = 8/105 (7%)
Query: 480 KPSKVVCVLNSVDPMDLKNEKFVTEIKEAMMYGTPIQSCGEVESVQIPLPNPDYRNDMSH 539
+ ++++C++N V +LK + EI E + G V S++IP P D H
Sbjct: 42 RATEILCLMNMVTEDELKADDEYEEILEDVR--DECSKYGIVRSLEIPRPYED------H 93
Query: 540 IHSSIGKVFIKFRDLVGAEKAMLVLPGSQFCQRTILCSYYSETDY 584
+GKVF++F ++A L G +F RT++ SYY Y
Sbjct: 94 PVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKY 138
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.312 0.129 0.376
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,257,330
Number of Sequences: 60738
Number of extensions: 1729118
Number of successful extensions: 4394
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 4383
Number of HSP's gapped (non-prelim): 10
length of query: 589
length of database: 30,389,216
effective HSP length: 112
effective length of query: 477
effective length of database: 23,586,560
effective search space: 11250789120
effective search space used: 11250789120
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)