ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactVI7159   suspect: LH   R     KOG4821  General function prediction only                               Predicted Na+-dependent cotransporter

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactVI7159 2495105  2496454 450  
         (450 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YMR034c [R] KOG4821 Predicted Na+-dependent cotransporter             515   e-146
Hs14149785 [R] KOG4821 Predicted Na+-dependent cotransporter           60   7e-09

>YMR034c [R] KOG4821 Predicted Na+-dependent cotransporter
          Length = 434

 Score =  515 bits (1326), Expect = e-146
 Identities = 254/408 (62%), Positives = 303/408 (74%), Gaps = 11/408 (2%)

Query: 37  IYNHKITQYIISQWFFIGLAIFIVLARFFPNFARSGGLIRGQYSIGYGAVIVIFLQSGLS 96
           I+ H +T+++ SQWFFI LAI IV+ARF PNFAR GGLI+GQYSIGYG V  IFLQSGL 
Sbjct: 11  IWAHSVTEFLKSQWFFICLAILIVIARFAPNFARDGGLIKGQYSIGYGCVAWIFLQSGLG 70

Query: 97  MSTKKLLVNMGNWRAHLVVLVISFLVTSSIMYGLCCXXXXXXXXXXXXWVLIGIIVTATC 156
           M ++ L+ NM NWRAH  +LV+SFL+TSSI+YG CC            WVLIG+I+TATC
Sbjct: 71  MKSRSLMANMLNWRAHATILVLSFLITSSIVYGFCCAVKAANDPKIDDWVLIGLILTATC 130

Query: 157 PTTVSSNVVMTTKADGNALLCLCEVFIGNVLGAFITPALVQMYTSSGPMVFGNPATDTSV 216
           PTTV+SNV+MTT A GN+LLC+CEVFIGN+LGAFITPALVQM+T+  P  +GNPAT   +
Sbjct: 131 PTTVASNVIMTTNAGGNSLLCVCEVFIGNLLGAFITPALVQMFTNRAPFAYGNPATGNGI 190

Query: 217 QQLYANVMKQIGLSVFIPLFVGQVLQNVFPKQVTWFLTTFRMN--KVGSFCLLLIMFSSF 274
             LY  VMKQ+GLSVF+PLFVGQV+QN FPK   ++L   +    K+GS+ LLLIMFSSF
Sbjct: 191 GALYGRVMKQVGLSVFVPLFVGQVIQNCFPKGTAYYLGFLKKYHIKIGSYMLLLIMFSSF 250

Query: 275 STAFYQHAFTSVSHACIIFLCFFNVGIYLFFTLVCFVCARPWFIIKIFDHEPTE-HSSKT 333
           STAFYQ AFTSVSH CIIFLCFFN+GIY+FFT + ++CARPWFI+K+F HEP E  S++ 
Sbjct: 251 STAFYQDAFTSVSHVCIIFLCFFNLGIYIFFTGLSYLCARPWFILKLFPHEPIEGKSTRL 310

Query: 334 YTICYKIFRPFYYSKRDAIAVMLCGAAKTAALGVSLISSQYGDDNPKLGTLLVPLVLYQS 393
           Y   Y IFRPFYYSK DAI +M CG AKTAALGVSLI+SQYGD    LG LLVPLVLYQ 
Sbjct: 311 YRYSYNIFRPFYYSKEDAICIMFCGPAKTAALGVSLITSQYGDKKEHLGKLLVPLVLYQV 370

Query: 394 EQVITANFMVPFMKKWASDEDEHGNKIIKQPTDEESRISQNKEDVSKE 441
           EQV+TANF V   K+W   +         Q    ES  +   E+V  E
Sbjct: 371 EQVMTANFFVSLFKRWIQKD--------AQADGSESSCANENEEVDLE 410


>Hs14149785 [R] KOG4821 Predicted Na+-dependent cotransporter
          Length = 159

 Score = 60.1 bits (144), Expect = 7e-09
 Identities = 34/147 (23%), Positives = 67/147 (45%), Gaps = 3/147 (2%)

Query: 41  KITQYIISQWFFIGLAIFIVLARFFPNFARSGGLIRGQYSIGYGAVIVIFLQSGLSMSTK 100
           ++ + +   WF +G+ + I  A+  P+   +GG ++ + ++ Y AV  IF  SGLS+ T+
Sbjct: 2   RLLERMRKDWFMVGIVLAIAGAKLEPSIGVNGGPLKPEITVSYIAVATIFFNSGLSLKTE 61

Query: 101 KLLVNMGNWRAHLVVLVISFLVTSSIMYGLCCXXXXXXXXXXXXWVLIGIIVTATCPTTV 160
           +L   + + + HL + + +     + ++                W+L G+      P  V
Sbjct: 62  ELTSALVHLKLHLFIQIFTLAFFPATIW---LFLQLLSITPINEWLLKGLQTVGCMPPPV 118

Query: 161 SSNVVMTTKADGNALLCLCEVFIGNVL 187
           SS V++T    GN    +     G+ L
Sbjct: 119 SSAVILTKAVGGNEAAAIFNSAFGSFL 145


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.327    0.139    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,235,539
Number of Sequences: 60738
Number of extensions: 979491
Number of successful extensions: 3845
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 3840
Number of HSP's gapped (non-prelim): 3
length of query: 450
length of database: 30,389,216
effective HSP length: 110
effective length of query: 340
effective length of database: 23,708,036
effective search space: 8060732240
effective search space used: 8060732240
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)