ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI7283 good E KOG3145 Amino acid transport and metabolism Cystine transporter Cystinosin
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI7283 2539961 2540710 250
(250 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YCR075c [E] KOG3145 Cystine transporter Cystinosin 192 5e-49
7300949 [E] KOG3145 Cystine transporter Cystinosin 77 2e-14
CE28541 [E] KOG3145 Cystine transporter Cystinosin 74 1e-13
At5g40670 [E] KOG3145 Cystine transporter Cystinosin 63 4e-10
Hs4826682 [E] KOG3145 Cystine transporter Cystinosin 49 7e-06
>YCR075c [E] KOG3145 Cystine transporter Cystinosin
Length = 260
Score = 192 bits (487), Expect = 5e-49
Identities = 101/247 (40%), Positives = 143/247 (57%), Gaps = 4/247 (1%)
Query: 1 MERVLGITYVICWSVSVYPVVWANWKNGNANAVSFDYVVLNTVGYSCLLASMVLQKLFWA 60
++ +LGI YV WS+S+YP + NW++ +A+A+S D+V+LNT GYS L+ S+ LQ W
Sbjct: 4 LDDILGIVYVTSWSISMYPPIITNWRHKSASAISMDFVMLNTAGYSYLVISIFLQLYCWK 63
Query: 61 EGTDET-LIAPEISGSDLIYCGHGTXXXXXXXXXXXXGNRLWNFSHTTRTHPKMHTVYKR 119
DE+ L P+++ D YC HG G R+W F + H KM+ Y R
Sbjct: 64 MTGDESDLGRPKLTQFDFWYCLHGCLMNVVLLTQVVAGARIWRFPG--KGHRKMNPWYLR 121
Query: 120 MFSXXXXXXXXXXXXXXXXXXXXGLSNATLLSYCNNLSLVKITMSLIKHLPQVRHNFQRK 179
+ N+ L+YCNNL L+KI+MSLIK++PQV HN RK
Sbjct: 122 ILLASLAIFSLLTVQFMYSNYWYDWHNSRTLAYCNNLFLLKISMSLIKYIPQVTHNSTRK 181
Query: 180 SMAGFPIQSTCIDVLGSVCSLSQLALRLASKPQGLTLMSFFTNFGKIGIGLITLLFNIVY 239
SM FPIQ +DV G + SL QL +L S QG +L +F TNFGK+G+ ++TL+FN ++
Sbjct: 182 SMDCFPIQGVFLDVTGGIASLLQLIWQL-SNDQGFSLDTFVTNFGKVGLSMVTLIFNFIF 240
Query: 240 VSQWIVY 246
+ QW VY
Sbjct: 241 IMQWFVY 247
>7300949 [E] KOG3145 Cystine transporter Cystinosin
Length = 363
Score = 77.0 bits (188), Expect = 2e-14
Identities = 58/249 (23%), Positives = 106/249 (42%), Gaps = 27/249 (10%)
Query: 4 VLGITYVICWSVSVYPVVWANWKNGNANAVSFDYVVLNTVGYSCLLASMVLQKLFWAEGT 63
+ G Y + WSVS YP +W+N++ + ++FD++ LN VG++ L SM L++ E
Sbjct: 134 IFGWVYFVAWSVSFYPQIWSNYRRKSVEGLNFDFLALNIVGFT--LYSMFNCGLYFIEDL 191
Query: 64 DET------LIAPEISGSDLIYCGHGTXXXXXXXXXXXXGNRLWNFSHTTRTHPKMHTVY 117
L + +D+++ H R
Sbjct: 192 QNEYEVRYPLGVNPVMLNDVVFSLHAMFATCITILQCFFYQR----------------AQ 235
Query: 118 KRMFSXXXXXXXXXXXXXXXXXXXXGLSNATLLSYCNNLSLVKITMSLIKHLPQVRHNFQ 177
+R+ G S L + S VK+T+++IK++PQ N++
Sbjct: 236 QRVSFIAYGILAIFAVVVVVSAGLAGGSVIHWLDFLYYCSYVKLTITIIKYVPQALMNYR 295
Query: 178 RKSMAGFPIQSTCIDVLGSVCSLSQLALRLASKPQGLTLMSFFTNFGKIGIGLITLLFNI 237
RKS +G+ I + +D G S+ Q+ L + +S F + K G+GL ++LF++
Sbjct: 296 RKSTSGWSIGNILLDFTGGTLSMLQMILNAHNYDD---WVSIFGDPTKFGLGLFSVLFDV 352
Query: 238 VYVSQWIVY 246
++ Q V+
Sbjct: 353 FFMLQHYVF 361
>CE28541 [E] KOG3145 Cystine transporter Cystinosin
Length = 404
Score = 74.3 bits (181), Expect = 1e-13
Identities = 58/248 (23%), Positives = 108/248 (43%), Gaps = 25/248 (10%)
Query: 3 RVLGITYVICWSVSVYPVVWANWKNGNANAVSFDYVVLNTVGYSCLLASMVLQKLFWAEG 62
+++G TY WS+S YP ++ N+K + ++FD++ LN VG+ C A L + +
Sbjct: 130 QIVGWTYFFAWSISFYPQMYLNFKRKSVVGLNFDFLSLNLVGF-CAYAIFNLLMYYNSHV 188
Query: 63 TDETLIA-----PEISGSDLIYCGHGTXXXXXXXXXXXXGNRLWNFSHTTRTHPKMHTVY 117
+E I P + +D+++ H R N S +++ M +
Sbjct: 189 KNEYNIVNPRSPPPVLLNDVVFAVHAFLACFITILQCLFYERD-NQSVSSKCIALMIVLI 247
Query: 118 KRMFSXXXXXXXXXXXXXXXXXXXXGLSNATLLSYCNNLSLVKITMSLIKHLPQVRHNFQ 177
F L LLS+ +LS +K+ ++ K+ PQ N+
Sbjct: 248 SFGFCSAAATV---------------LRKIQLLSFVTSLSYIKMAVTCCKYFPQAYFNYT 292
Query: 178 RKSMAGFPIQSTCIDVLGSVCSLSQLALRLASKPQGLTLMSFFTNFGKIGIGLITLLFNI 237
RKS G+ I + +D G + Q+ L+ + +F+ N K G+G +++ F+I
Sbjct: 293 RKSTVGWSIGNIMLDFTGGTLDILQMILQAVNVND---WSAFYANPVKFGLGFVSIFFDI 349
Query: 238 VYVSQWIV 245
+++ Q V
Sbjct: 350 IFMVQHYV 357
>At5g40670 [E] KOG3145 Cystine transporter Cystinosin
Length = 270
Score = 62.8 bits (151), Expect = 4e-10
Identities = 52/252 (20%), Positives = 106/252 (41%), Gaps = 35/252 (13%)
Query: 4 VLGITYVICWSVSVYPVVWANWKNGNANAVSFDYVVLNTVGYS-------CLLASMVLQK 56
++G WS+S YP + N++ + ++FD+V+LN +S CL S V+QK
Sbjct: 15 IVGWIAFASWSISFYPQLILNFRRRSVVGLNFDFVMLNLTKHSSYMIYNVCLYFSPVIQK 74
Query: 57 LFWAEGTDETLIAPEISGSDLIYCGHGTXXXXXXXXXXXXGNRLWNFSHTTRTHPKMHTV 116
++ D+ +I ++ +D+ + H +
Sbjct: 75 QYFDTYGDKEMIP--VAANDVAFSIHAVVMTAVTLFQIF--------------------I 112
Query: 117 YKR---MFSXXXXXXXXXXXXXXXXXXXXGLSNATLLSYCNNLSLVKITMSLIKHLPQVR 173
Y+R S L + L + + +++ M+ +K++PQ +
Sbjct: 113 YERGPQKVSRLAIGIVVVVWGFAAICFFIALPTHSWLWLISIFNSIQVFMTCVKYIPQAK 172
Query: 174 HNFQRKSMAGFPIQSTCIDVLGSVCSLSQLALRLASKPQGLTLMSFFTNFGKIGIGLITL 233
NF RKS G+ I + +D G + + Q+ ++ + + +F+ N GK + LI++
Sbjct: 173 MNFTRKSTVGWSIGNILLDFTGGLANYLQMVIQSIDQN---SWKNFYGNMGKTLLSLISI 229
Query: 234 LFNIVYVSQWIV 245
F+I+++ Q V
Sbjct: 230 FFDILFMFQHYV 241
>Hs4826682 [E] KOG3145 Cystine transporter Cystinosin
Length = 367
Score = 48.9 bits (115), Expect = 7e-06
Identities = 28/95 (29%), Positives = 51/95 (53%), Gaps = 3/95 (3%)
Query: 148 TLLSYCNNLSLVKITMSLIKHLPQVRHNFQRKSMAGFPIQSTCIDVLGSVCSLSQLALRL 207
T L + S +K+ ++L+K+ PQ NF KS G+ I + +D G SL Q+ L+
Sbjct: 261 TWLQFLFCFSYIKLAVTLVKYFPQAYMNFYYKSTEGWSIGNVLLDFTGGSFSLLQMFLQS 320
Query: 208 ASKPQGLTLMSFFTNFGKIGIGLITLLFNIVYVSQ 242
+ Q + F + K G+G+ +++F++V+ Q
Sbjct: 321 YNNDQWTLI---FGDPTKFGLGVFSIVFDVVFFIQ 352
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.324 0.137 0.430
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,138,933
Number of Sequences: 60738
Number of extensions: 455558
Number of successful extensions: 1211
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1197
Number of HSP's gapped (non-prelim): 13
length of query: 250
length of database: 30,389,216
effective HSP length: 104
effective length of query: 146
effective length of database: 24,072,464
effective search space: 3514579744
effective search space used: 3514579744
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)