ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI7346 good E KOG0456 Amino acid transport and metabolism Aspartate kinase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI7346 2558178 2559743 522
(522 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YER052c [E] KOG0456 Aspartate kinase 732 0.0
SPBC19F5.04 [E] KOG0456 Aspartate kinase 506 e-143
At5g13280 [E] KOG0456 Aspartate kinase 200 5e-51
At5g14060 [E] KOG0456 Aspartate kinase 196 5e-50
At3g02020 [E] KOG0456 Aspartate kinase 194 4e-49
>YER052c [E] KOG0456 Aspartate kinase
Length = 527
Score = 732 bits (1889), Expect = 0.0
Identities = 368/522 (70%), Positives = 448/522 (85%), Gaps = 10/522 (1%)
Query: 5 SNWVVQKFGGTSIGKFSKQIVNEIIKVYSQEY--DNNVAVVCSARSSYTKAEGTTSRLLL 62
SNWVVQKFGGTS+GKF QIV++I+K YS+ +NNVAVVCSARSSYTKAEGTTSRLL
Sbjct: 12 SNWVVQKFGGTSVGKFPVQIVDDIVKHYSKPDGPNNNVAVVCSARSSYTKAEGTTSRLLK 71
Query: 63 ACDLASNNNYEYKNVLDLVERDHVANAEESVKDITIQETLIEDTKREINIVRRYLDASQV 122
CDLAS + E++++++++ +DH+ NA+ + + +Q L++DT +E+ +V++YL+AS+V
Sbjct: 72 CCDLASQES-EFQDIIEVIRQDHIDNADRFILNPALQAKLVDDTNKELELVKKYLNASKV 130
Query: 123 LGEVSTRTLDLVMSLGEKLSCLYVTALCQDNGVNARYVDLSFIVPSDYECKEGTLDNSFY 182
LGEVS+RT+DLVMS GEKLSCL++TALC D G A+YVDLS IVPSD+ LDNSFY
Sbjct: 131 LGEVSSRTVDLVMSCGEKLSCLFMTALCNDRGCKAKYVDLSHIVPSDFSAS--ALDNSFY 188
Query: 183 TFLVTAFREKLSPILKETENGTKIIPVITGFFGVVPMGLLNGVGRGYTDLCAALIAVALN 242
TFLV A +EKL+P + E +I+PV TGFFG+VP GLLNGVGRGYTDLCAALIAVA+N
Sbjct: 189 TFLVQALKEKLAPFVSAKE---RIVPVFTGFFGLVPTGLLNGVGRGYTDLCAALIAVAVN 245
Query: 243 ADELQVWKEVDGIFTADPRKVPQARLLDTVTPDEAAELTYYGSEVIHPFTMEQVIRTHIP 302
ADELQVWKEVDGIFTADPRKVP+ARLLD+VTP+EA+ELTYYGSEVIHPFTMEQVIR IP
Sbjct: 246 ADELQVWKEVDGIFTADPRKVPEARLLDSVTPEEASELTYYGSEVIHPFTMEQVIRAKIP 305
Query: 303 IRIKNVQNPKGNGTIIYPSDQAKKGESTPPHPPVALTSSIFESKKKGATAITTKSDIVVV 362
IRIKNVQNP GNGTIIYP + AKKGESTPPHPP L+SS +E +K+GATAITTK+DI V+
Sbjct: 306 IRIKNVQNPLGNGTIIYPDNVAKKGESTPPHPPENLSSSFYEKRKRGATAITTKNDIFVI 365
Query: 363 NIHSNKKTLSHGFLAEMFSTLDKYKLVVDLISTSEVHVSMALPVPDSDSLKALRCAVEEL 422
NIHSNKKTLSHGFLA++F+ LDKYKLVVDLISTSEVHVSMALP+PD+DSLK+LR A E+L
Sbjct: 366 NIHSNKKTLSHGFLAQIFTILDKYKLVVDLISTSEVHVSMALPIPDADSLKSLRQAEEKL 425
Query: 423 KRLGTVDVIKKMAIVSLVGKQMKQFVGIAGTMFTTLAEQHINIEMISQGANEINISCVID 482
+ LG+VD+ KK++IVSLVGK MKQ++GIAGTMFTTLAE+ INIEMISQGANEINISCVI+
Sbjct: 426 RILGSVDITKKLSIVSLVGKHMKQYIGIAGTMFTTLAEEGINIEMISQGANEINISCVIN 485
Query: 483 ESNAVRALQSIHAKLLDSPQN--ENFESAVNERLEQIKQMSL 522
ES++++ALQ IHAKLL N FE A++ERLEQ+K++ +
Sbjct: 486 ESDSIKALQCIHAKLLSERTNTSNQFEHAIDERLEQLKRLGI 527
>SPBC19F5.04 [E] KOG0456 Aspartate kinase
Length = 519
Score = 506 bits (1303), Expect = e-143
Identities = 269/498 (54%), Positives = 352/498 (70%), Gaps = 15/498 (3%)
Query: 4 TSNWVVQKFGGTSIGKFSKQIVNEIIKVYSQEYDNNVAVVCSARSSYTKAEGTTSRLLLA 63
+ WVVQKFGGTS+GKF +I ++ K Y VA+VCSARS+ TKAEGTT+RL+ A
Sbjct: 12 SKGWVVQKFGGTSVGKFPIKIAVDVAKEYLST--KRVALVCSARSTDTKAEGTTTRLIRA 69
Query: 64 CDLASNNNY-EYKNVLDLVERDHVANAEESVKDITIQETLIEDTKREINIVRRYLDASQV 122
+ A +++ ++E DHV A + ++D+ IQ+ LI+ + + +YL+A +V
Sbjct: 70 TEAALRPAVGSVHDLVRIIETDHVQAARDFIQDVGIQDELIDAFHADCVELEQYLNAIRV 129
Query: 123 LGEVSTRTLDLVMSLGEKLSCLYVTALCQDNGVNARYVDLSFIVPSDYECKEGTLDNSFY 182
L EVS RT DLV+ +GE+LSC ++ A+ +D G+++ ++D+S I+ E + LD SFY
Sbjct: 130 LSEVSPRTRDLVIGMGERLSCRFMAAVLKDQGIDSEFIDMSHIIDEQREWRN--LDASFY 187
Query: 183 TFLVTAFREKLSPILKETENGTKIIPVITGFFGVVPMGLLNGVGRGYTDLCAALIAVALN 242
+L + K++ + G K+ PV+TGFFG+VP GLL+ +GRGYTD CAAL+AV LN
Sbjct: 188 AYLASQLASKVTAV------GNKV-PVVTGFFGMVPGGLLSQIGRGYTDFCAALLAVGLN 240
Query: 243 ADELQVWKEVDGIFTADPRKVPQARLLDTVTPDEAAELTYYGSEVIHPFTMEQVIRTHIP 302
ADELQ+WKEVDGIFTADPRKVP ARLL +TP+EAAELTYYGSEVIHPFTM QV+ IP
Sbjct: 241 ADELQIWKEVDGIFTADPRKVPTARLLPLITPEEAAELTYYGSEVIHPFTMSQVVHARIP 300
Query: 303 IRIKNVQNPKGNGTIIYPSDQAKKGESTPPHPPVALTSSIFES-KKKGATAITTKSDIVV 361
IRIKNV NP+G GT+I+P ++ G +TPPHPP + I S KGATA+T K I+V
Sbjct: 301 IRIKNVGNPRGKGTVIFPDTISRHGSATPPHPPKIMPDDISASLANKGATAVTIKDTIMV 360
Query: 362 VNIHSNKKTLSHGFLAEMFSTLDKYKLVVDLISTSEVHVSMALPVPDSDSLKALRCAVEE 421
+NI SN+K +HGFLA +F+ LDKYKL VDLI+TSEVHVSMAL D + A E
Sbjct: 361 INIQSNRKISAHGFLASIFAILDKYKLAVDLITTSEVHVSMALYEESDDG--NMHEAFVE 418
Query: 422 LKRLGTVDVIKKMAIVSLVGKQMKQFVGIAGTMFTTLAEQHINIEMISQGANEINISCVI 481
L+RLGT+D++ +AI+SLVGK M+ G AG MF LAE INIEMISQGA+EINISCVI
Sbjct: 419 LRRLGTLDILHGLAILSLVGKHMRNTTGYAGRMFCKLAEAQINIEMISQGASEINISCVI 478
Query: 482 DESNAVRALQSIHAKLLD 499
DE AV+AL IH +LL+
Sbjct: 479 DEKMAVKALNVIHKELLE 496
>At5g13280 [E] KOG0456 Aspartate kinase
Length = 569
Score = 200 bits (508), Expect = 5e-51
Identities = 153/507 (30%), Positives = 255/507 (50%), Gaps = 66/507 (13%)
Query: 9 VQKFGGTSIGKFSK-QIVNEIIKVYSQEYDNNVAVVCSARSSYTKAEGTTSRLLLACDLA 67
V KFGG+S+ + + V ++I + +E + +V SA TT+ LLLA + A
Sbjct: 89 VMKFGGSSVASAERMKEVADLILTFPEE---SPVIVLSAMGK------TTNNLLLAGEKA 139
Query: 68 SN---NNYEYKNVLDLVERDHVANAEESVKDITIQETLIEDTKREINIVRRYLDASQVLG 124
+ +N L +++ H+ +E D ++ T +E+ + + L ++
Sbjct: 140 VSCGVSNASEIEELSIIKELHIRTVKELNIDPSVILTYLEE-------LEQLLKGIAMMK 192
Query: 125 EVSTRTLDLVMSLGEKLSCLYVTALCQDNGVNARYVD---LSFIVPSDYECKEGTLDNSF 181
E++ RT D ++S GE LS A GV AR D + FI D+ +
Sbjct: 193 ELTLRTRDYLVSFGECLSTRIFAAYLNTIGVKARQYDAFEIGFITTDDFTNGD------- 245
Query: 182 YTFLVTAFREKLSPILKETENGTKIIPVITGFFGVV-PMGLLNGVGRGYTDLCAALIAVA 240
++ A ++ L + +P++TGF G G + +GRG +DL A I A
Sbjct: 246 ---ILEATYPAVAKRLYDDWMHDPAVPIVTGFLGKGWKTGAVTTLGRGGSDLTATTIGKA 302
Query: 241 LNADELQVWKEVDGIFTADPRKVPQARLLDTVTPDEAAELTYYGSEVIHPFTMEQVIRTH 300
L E+QVWK+VDG+ T DP +A + +T DEAAEL Y+G++V+HP +M
Sbjct: 303 LGLKEIQVWKDVDGVLTCDPTIYKRATPVPYLTFDEAAELAYFGAQVLHPQSMRPAREGE 362
Query: 301 IPIRIKNVQNPKGNGTIIYPSDQAKKGESTPPHPPVALTSSIFESKKKGATAITTKSDIV 360
IP+R+KN NPK GTII + +T SI T+I K ++
Sbjct: 363 IPVRVKNSYNPKAPGTIITKTRD--------------MTKSIL-------TSIVLKRNVT 401
Query: 361 VVNIHSNKKTLSHGFLAEMFSTLDKYKLVVDLISTSEVHVSMALPVPDSDSL-------K 413
+++I S + GFLA++FS ++ + VD+++TSEV +S+ L D L +
Sbjct: 402 MLDIASTRMLGQVGFLAKVFSIFEELGISVDVVATSEVSISLTL---DPSKLWSRELIQQ 458
Query: 414 ALRCAVEELKRLGTVDVIKKMAIVSLVGKQMKQFVGIAGTMFTTLAEQHINIEMISQGAN 473
L VEEL+++ V+++K AI+SL+G + I F L + +N++MISQGA+
Sbjct: 459 ELDHVVEELEKIAVVNLLKGRAIISLIGNVQHSSL-ILERAFHVLYTKGVNVQMISQGAS 517
Query: 474 EINISCVIDESNAVRALQSIHAKLLDS 500
++NIS +++E+ A +Q++H +S
Sbjct: 518 KVNISFIVNEAEAEGCVQALHKSFFES 544
>At5g14060 [E] KOG0456 Aspartate kinase
Length = 544
Score = 196 bits (499), Expect = 5e-50
Identities = 143/505 (28%), Positives = 249/505 (48%), Gaps = 60/505 (11%)
Query: 9 VQKFGGTSIGKFSKQIVNEIIKVYSQEYDNNVAVVCSARSSYTKAEGTTSRLLLACDLAS 68
V KFGG+S+ S + + E+ + D +V SA TT++LL A + A
Sbjct: 85 VMKFGGSSVE--SAERMKEVANLILSFPDERPVIVLSAMGK------TTNKLLKAGEKAV 136
Query: 69 N---NNYEYKNVLDLVERDHVANAEESVKDITIQETLIEDTKREINIVRRYLDASQVLGE 125
N E L ++ H+ A E + ++ T+IE + + + + L ++ E
Sbjct: 137 TCGVTNVESIEELSFIKELHLRTAHE----LGVETTVIE---KHLEGLHQLLKGISMMKE 189
Query: 126 VSTRTLDLVMSLGEKLSCLYVTALCQDNGVNARYVD---LSFIVPSDYECKEGTLDNSFY 182
++ RT D ++S GE +S +A G AR D + FI D+ +
Sbjct: 190 LTLRTRDYLVSFGECMSTRLFSAYLNKIGHKARQYDAFEIGFITTDDFTNAD-------- 241
Query: 183 TFLVTAFREKLSPILKETENGTKIIPVITGFFGVVPMGL-LNGVGRGYTDLCAALIAVAL 241
++ A +S L + +PV+TG+ G + +GRG +DL A I AL
Sbjct: 242 --ILEATYPAVSKTLVGDWSKENAVPVVTGYLGKGWRSCAITTLGRGGSDLTATTIGKAL 299
Query: 242 NADELQVWKEVDGIFTADPRKVPQARLLDTVTPDEAAELTYYGSEVIHPFTMEQVIRTHI 301
E+QVWK+VDG+ T DP P A+ + +T DEAAEL Y+G++V+HP +M I
Sbjct: 300 GLREIQVWKDVDGVLTCDPNIYPGAQSVPYLTFDEAAELAYFGAQVLHPLSMRPARDGDI 359
Query: 302 PIRIKNVQNPKGNGTIIYPSDQAKKGESTPPHPPVALTSSIFESKKKGATAITTKSDIVV 361
P+R+KN NP GT+I S + K T+I K ++ +
Sbjct: 360 PVRVKNSYNPTAPGTVITRSR---------------------DMSKAVLTSIVLKRNVTM 398
Query: 362 VNIHSNKKTLSHGFLAEMFSTLDKYKLVVDLISTSEVHVSMALPVPD------SDSLKAL 415
++I S + +GFLA++F+T + + VD+++TSEV +S+ L + L
Sbjct: 399 LDIASTRMLGQYGFLAKVFTTFEDLGISVDVVATSEVSISLTLDPAKLWGRELIQRVNEL 458
Query: 416 RCAVEELKRLGTVDVIKKMAIVSLVGKQMKQFVGIAGTMFTTLAEQHINIEMISQGANEI 475
VEEL+++ V ++++ +I+SL+G K + I +F +N++MISQGA+++
Sbjct: 459 DNLVEELEKIAVVKLLQRRSIISLIGNVQKSSL-ILEKVFQVFRSNGVNVQMISQGASKV 517
Query: 476 NISCVIDESNAVRALQSIHAKLLDS 500
NIS ++++ A + ++++H+ ++
Sbjct: 518 NISLIVNDEEAEQCVRALHSAFFET 542
>At3g02020 [E] KOG0456 Aspartate kinase
Length = 559
Score = 194 bits (492), Expect = 4e-49
Identities = 145/501 (28%), Positives = 250/501 (48%), Gaps = 54/501 (10%)
Query: 9 VQKFGGTSIGKFSKQIVNEIIKVYSQEYDNNVAVVCSARSSYTKAEGTTSRLLLACDLA- 67
V KFGG+S+ + I ++ K+ D VV SA + TT++LL+A + A
Sbjct: 86 VMKFGGSSVASAERMI--QVAKLILSFPDEKPVVVLSAMAK------TTNKLLMAGEKAV 137
Query: 68 --SNNNYEYKNVLDLVERDHVANAEESVKDITIQETLIEDTKREINIVRRYLDASQVLGE 125
N + L ++ H+ A E + ++ +I + + + + L ++ E
Sbjct: 138 CCGVTNVDTIEELSYIKELHIRTAHE----LGVETAVIAE---HLEGLEQLLKGVAMMKE 190
Query: 126 VSTRTLDLVMSLGEKLSCLYVTALCQDNGVNARYVDLSFIVPSDYECKEGTLDNSFYTFL 185
++ R+ D ++S GE +S A G AR D +E T D+ +
Sbjct: 191 LTLRSRDYLVSFGECMSTRLFAAYLNKIGHKARQYDA-------FEIGIITTDDFTNADI 243
Query: 186 VTAFREKLSPILKETENGTKIIPVITGFFGVVPMGL-LNGVGRGYTDLCAALIAVALNAD 244
+ A +S L + +PV+TGF G + +GRG +DL A I AL
Sbjct: 244 LEATYPAVSKKLLGDWSKENALPVVTGFLGKGWRSCAVTTLGRGGSDLTATTIGKALGLR 303
Query: 245 ELQVWKEVDGIFTADPRKVPQARLLDTVTPDEAAELTYYGSEVIHPFTMEQVIRTHIPIR 304
E+QVWK+VDG+ T DP A+ + +T DEAAEL Y+G++V+HP +M +IP+R
Sbjct: 304 EIQVWKDVDGVLTCDPNIYCGAQPVPHLTFDEAAELAYFGAQVLHPLSMRPAREGNIPVR 363
Query: 305 IKNVQNPKGNGTIIYPSDQAKKGESTPPHPPVALTSSIFESKKKGATAITTKSDIVVVNI 364
+KN NP GT+I S + K T+I K ++ +++I
Sbjct: 364 VKNSYNPTAPGTVITRSR---------------------DMSKAVLTSIVLKRNVTMLDI 402
Query: 365 HSNKKTLSHGFLAEMFSTLDKYKLVVDLISTSEVHVSMALPVPDSDSLKALR----CAVE 420
S + +GFLA++FST +K + VD+++TSEV +S+ L S + ++ VE
Sbjct: 403 TSTRMLGQYGFLAKVFSTFEKLGISVDVVATSEVSISLTLDPSKFCSRELIQHELDQVVE 462
Query: 421 ELKRLGTVDVIKKMAIVSLVGK-QMKQFVGIAGTMFTTLAEQHINIEMISQGANEINISC 479
EL+++ V++++ +I+SL+G Q F+ G F L IN++MISQGA+++NIS
Sbjct: 463 ELEKIAVVNLLRHRSIISLIGNVQRSSFILEKG--FRVLRTNGINVQMISQGASKVNISL 520
Query: 480 VIDESNAVRALQSIHAKLLDS 500
++++ A ++++H+ ++
Sbjct: 521 IVNDDEAEHCVKALHSAFFET 541
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.133 0.366
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,338,594
Number of Sequences: 60738
Number of extensions: 1228723
Number of successful extensions: 3725
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3699
Number of HSP's gapped (non-prelim): 5
length of query: 522
length of database: 30,389,216
effective HSP length: 111
effective length of query: 411
effective length of database: 23,647,298
effective search space: 9719039478
effective search space used: 9719039478
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)