BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0063 (cca) DE:tRNA nucleotidyltransferase (cca)
(448 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||D75197 tRNA nucleotidyltransferase (cca) PAB0063 - Pyrococc... 896 0.0
pir||C71230 hypothetical protein PH0101 - Pyrococcus horikoshii ... 675 0.0
sp|O26684|CCA_METTH TRNA NUCLEOTIDYLTRANSFERASE (TRNA ADENYLYLTR... 249 5e-65
gi|11499739 tRNA nucleotidyltransferase (cca) [Archaeoglobus ful... 243 4e-63
sp|Q58511|CCA_METJA TRNA NUCLEOTIDYLTRANSFERASE (TRNA ADENYLYLTR... 236 3e-61
gb|AAG18757.1| (AE004980) tRNA nucleotidyltransferase; Cca [Halo... 205 1e-51
emb|CAC12566.1| (AL445067) trna nucleotidyltransferase related p... 197 2e-49
gb|AAK41301.1| tRNA nucleotidyltransferase [Sulfolobus solfatari... 197 3e-49
sp|P77978|CCA_SULSH TRNA NUCLEOTIDYLTRANSFERASE (TRNA ADENYLYLTR... 192 9e-48
pir||F64438 hypothetical protein MJ1111 - Methanococcus jannaschii 188 1e-46
pir||T37335 probable tRNA adenylyltransferase (EC 2.7.7.25) - Th... 145 8e-34
pir||E72563 probable tRNA nucleotidyltransferase APE1791 - Aerop... 125 1e-27
>pir||D75197 tRNA nucleotidyltransferase (cca) PAB0063 - Pyrococcus abyssi
(strain Orsay) >gi|5457536|emb|CAB49027.1| (AJ248283)
tRNA nucleotidyltransferase (cca) [Pyrococcus abyssi]
Length = 448
Score = 896 bits (2289), Expect = 0.0
Identities = 448/448 (100%), Positives = 448/448 (100%)
Query: 1 MLKMTLKEVLESIKPKDEERKKVKLIMDELRGIAQEVIEESGEEIEVKFVGSLAKDTYLS 60
MLKMTLKEVLESIKPKDEERKKVKLIMDELRGIAQEVIEESGEEIEVKFVGSLAKDTYLS
Sbjct: 1 MLKMTLKEVLESIKPKDEERKKVKLIMDELRGIAQEVIEESGEEIEVKFVGSLAKDTYLS 60
Query: 61 GDHDIDMFLAFPLSIPVEKLKSKGLEIAESIGKRLESYEISYAEHPYVRGVYKGYQVDIV 120
GDHDIDMFLAFPLSIPVEKLKSKGLEIAESIGKRLESYEISYAEHPYVRGVYKGYQVDIV
Sbjct: 61 GDHDIDMFLAFPLSIPVEKLKSKGLEIAESIGKRLESYEISYAEHPYVRGVYKGYQVDIV 120
Query: 121 PCYNVRDWREVRTAVDRSILHTEWVLKNIKGKNDEVRLLKRFLKGINAYGSEVYRRGFSG 180
PCYNVRDWREVRTAVDRSILHTEWVLKNIKGKNDEVRLLKRFLKGINAYGSEVYRRGFSG
Sbjct: 121 PCYNVRDWREVRTAVDRSILHTEWVLKNIKGKNDEVRLLKRFLKGINAYGSEVYRRGFSG 180
Query: 181 YLAEILVIKFGSFLKVLEKADFMLRQKIIDPENWLKREPEIAMKTVKREIEEDKPIIVID 240
YLAEILVIKFGSFLKVLEKADFMLRQKIIDPENWLKREPEIAMKTVKREIEEDKPIIVID
Sbjct: 181 YLAEILVIKFGSFLKVLEKADFMLRQKIIDPENWLKREPEIAMKTVKREIEEDKPIIVID 240
Query: 241 PVDPRRNVAANLSWERYGLFYFKAREFLTKPSTELFFPRDKKGNYLEVLRRKGTHLVTLT 300
PVDPRRNVAANLSWERYGLFYFKAREFLTKPSTELFFPRDKKGNYLEVLRRKGTHLVTLT
Sbjct: 241 PVDPRRNVAANLSWERYGLFYFKAREFLTKPSTELFFPRDKKGNYLEVLRRKGTHLVTLT 300
Query: 301 FEPPNLVDDIIIPQVERTAKGLARQLELEGFRVLGIDYGRDFIFLEVEEIERPRIKIKKG 360
FEPPNLVDDIIIPQVERTAKGLARQLELEGFRVLGIDYGRDFIFLEVEEIERPRIKIKKG
Sbjct: 301 FEPPNLVDDIIIPQVERTAKGLARQLELEGFRVLGIDYGRDFIFLEVEEIERPRIKIKKG 360
Query: 361 PLYFTHHGLRFFDKNDIVWIEGKELASEKSSLGFIVDVLEDILRKGQFSAGKNVKDAIVG 420
PLYFTHHGLRFFDKNDIVWIEGKELASEKSSLGFIVDVLEDILRKGQFSAGKNVKDAIVG
Sbjct: 361 PLYFTHHGLRFFDKNDIVWIEGKELASEKSSLGFIVDVLEDILRKGQFSAGKNVKDAIVG 420
Query: 421 ANIIIDFVPKALAQEAYLFLSREKFRVK 448
ANIIIDFVPKALAQEAYLFLSREKFRVK
Sbjct: 421 ANIIIDFVPKALAQEAYLFLSREKFRVK 448
>pir||C71230 hypothetical protein PH0101 - Pyrococcus horikoshii
>gi|3256487|dbj|BAA29170.1| (AP000001) 481aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 481
Score = 675 bits (1723), Expect = 0.0
Identities = 320/443 (72%), Positives = 382/443 (85%)
Query: 6 LKEVLESIKPKDEERKKVKLIMDELRGIAQEVIEESGEEIEVKFVGSLAKDTYLSGDHDI 65
+ EVL+ I P EE V +M+E++ +E IEE + + FVGSLAKDTYL+GDHD+
Sbjct: 39 IAEVLQRIVPTKEEENFVSTLMEEIKEKTEETIEELNLDAKPYFVGSLAKDTYLAGDHDV 98
Query: 66 DMFLAFPLSIPVEKLKSKGLEIAESIGKRLESYEISYAEHPYVRGVYKGYQVDIVPCYNV 125
D+F+AFPL +E+L+ KGLE+ + +G+RL YEI+YAEHPYVR YKG +VDIVPCYNV
Sbjct: 99 DLFIAFPLDTSLEELREKGLELGKVLGERLGKYEIAYAEHPYVRAEYKGIRVDIVPCYNV 158
Query: 126 RDWREVRTAVDRSILHTEWVLKNIKGKNDEVRLLKRFLKGINAYGSEVYRRGFSGYLAEI 185
++W++VRTAVDRSILHT WVL+NIKGKN+EVRLLKRFLKGINAYGSE+Y RGFSGYLAEI
Sbjct: 159 KNWKDVRTAVDRSILHTHWVLENIKGKNNEVRLLKRFLKGINAYGSEIYIRGFSGYLAEI 218
Query: 186 LVIKFGSFLKVLEKADFMLRQKIIDPENWLKREPEIAMKTVKREIEEDKPIIVIDPVDPR 245
L+I+FGSFL VLEK+DFMLR+KIIDPE+W+KRE EI MKT+KRE+ EDKP+IVIDPVDPR
Sbjct: 219 LIIEFGSFLNVLEKSDFMLRKKIIDPEDWMKRESEITMKTIKREVGEDKPLIVIDPVDPR 278
Query: 246 RNVAANLSWERYGLFYFKAREFLTKPSTELFFPRDKKGNYLEVLRRKGTHLVTLTFEPPN 305
RNVAANLSWERYGLFYFK++EFL +PSTE FFPR K GNYL LR + THL+TLTF PP
Sbjct: 279 RNVAANLSWERYGLFYFKSQEFLKEPSTEFFFPRKKIGNYLSALRSRKTHLITLTFNPPK 338
Query: 306 LVDDIIIPQVERTAKGLARQLELEGFRVLGIDYGRDFIFLEVEEIERPRIKIKKGPLYFT 365
LVDDI++PQVERTAKG+ RQLELEGF+VLG DYGR+FIFLEV+ +ER IK+K+GPLYFT
Sbjct: 339 LVDDILLPQVERTAKGIKRQLELEGFKVLGYDYGREFIFLEVDRLEREGIKVKRGPLYFT 398
Query: 366 HHGLRFFDKNDIVWIEGKELASEKSSLGFIVDVLEDILRKGQFSAGKNVKDAIVGANIII 425
HG RF++KNDIVWIEGKELASEK FIVDVLE+ILRKGQFSAGKN+KDAIV +I++
Sbjct: 399 QHGKRFYEKNDIVWIEGKELASEKPISTFIVDVLEEILRKGQFSAGKNIKDAIVKGDILV 458
Query: 426 DFVPKALAQEAYLFLSREKFRVK 448
DFVPK L+++AYLFLSREKFRVK
Sbjct: 459 DFVPKELSKDAYLFLSREKFRVK 481
>sp|O26684|CCA_METTH TRNA NUCLEOTIDYLTRANSFERASE (TRNA ADENYLYLTRANSFERASE) (TRNA
CCA-PYROPHOSPHORYLASE) (CCA-ADDING ENZYME)
>gi|7446769|pir||D69177 hypothetical protein MTH584 -
Methanobacterium thermoautotrophicum (strain Delta H)
>gi|2621662|gb|AAB85090.1| (AE000840) tRNA
nucleotidyltransferase [Methanobacterium
thermoautotrophicum]
Length = 454
Score = 249 bits (629), Expect = 5e-65
Identities = 156/417 (37%), Positives = 232/417 (55%), Gaps = 30/417 (7%)
Query: 9 VLESIKPKDEERKKVKLIMDELRGIAQEVIEESGEEIEVKFVGSLAKDTYLSGDHDIDMF 68
+L++IKP +E ++V + D L + EE G + E VGS+AK T+LSG DID+F
Sbjct: 8 ILKTIKPDGDEYRRVMELSDSLVECLNGLAEEQGIDAEAVLVGSVAKGTWLSGAADIDIF 67
Query: 69 LAFPLSIPVEKLKSKGLEIAESIGKRL-ESYEISYAEHPYVRGVYKGYQVDIVPCYNVRD 127
+ FPL+ P ++LK GL + +R E YA HPYV G GY+VD VPCY + D
Sbjct: 68 IHFPLTTPEDELKESGLRLGYGCIERFGGEAEERYASHPYVTGYIDGYEVDFVPCYRIDD 127
Query: 128 WREVRTAVDRSILHTEWVLKNIKG-KNDEVRLLKRFLKGINAYGSEVYRRGFSGYLAEIL 186
+R+AVDR+ILHT ++ +N++ + D+V LLK+F+K YGSE GF+GYLAE+L
Sbjct: 128 ASMLRSAVDRTILHTRYIQENLRDEQRDDVLLLKQFMKSTGTYGSEFRVGGFAGYLAELL 187
Query: 187 VIKFGSFLKVLEKADFMLRQKIIDPENWLKREPEIAMKTVKREIEEDKPIIVIDPVDPRR 246
V+++G F VLE A IID E + E+ P++V+DPVD R
Sbjct: 188 VLRYGDFEGVLEGALQWRPGYIIDLEG----------HGTGKGFED--PLVVVDPVDRNR 235
Query: 247 NVAANLSWERYGLFYFKAREFLTKPSTELFFPRDKKGNYLEV---LRRKGTHLVTLTFEP 303
NVAA L+ +R F + FL PS E F P + E+ L +G+ ++ ++
Sbjct: 236 NVAAALTLQRMAEFVTASLNFLGNPSPEYFQPPSYSTDAAEITGTLSCRGSRVIVISTGV 295
Query: 304 PNLVDDIIIPQVERTAKGLARQLELEGFRVLGIDYGRD-----FIFLEVEEIERPRIKIK 358
P++ D + PQ+ +T + + LE EGF VLG DY D F+ LE++ E P +
Sbjct: 296 PDVPSDALYPQLRKTLDSVVKNLEAEGFSVLGADYWSDESKSTFMVLEMDVWELPSYLKR 355
Query: 359 KG-PLYFTHHGLRFFDKNDIVWIEGKELASE-----KSSLGFIVDVLE--DILRKGQ 407
G P++ H RF K++ VW+EG L E +S++ ++ D+L D LR G+
Sbjct: 356 YGPPVWSRRHRERFLRKHERVWVEGSRLTIESPRRHRSAVSYLRDLLSKPDRLRMGK 412
>gi|11499739 tRNA nucleotidyltransferase (cca) [Archaeoglobus fulgidus]
>gi|3913198|sp|O28126|CCA_ARCFU TRNA
NUCLEOTIDYLTRANSFERASE (TRNA ADENYLYLTRANSFERASE) (TRNA
CCA-PYROPHOSPHORYLASE) (CCA-ADDING ENZYME)
>gi|7446770|pir||D69519 tRNA nucleotidyltransferase
(cca) homolog - Archaeoglobus fulgidus
>gi|2648358|gb|AAB89084.1| (AE000955) tRNA
nucleotidyltransferase (cca) [Archaeoglobus fulgidus]
Length = 437
Score = 243 bits (613), Expect = 4e-63
Identities = 141/375 (37%), Positives = 216/375 (57%), Gaps = 31/375 (8%)
Query: 6 LKEVLESIKPKDEERKKVKLIMDELRGIAQEVIEESGEEIEVKFVGSLAKDTYLSGDHDI 65
L++ LE + P +EE +K + +ELR ++E G +E FVGS A++T+L G +I
Sbjct: 7 LEKALELVIPDEEEVRKGREAEEELR----RRLDELG--VEYVFVGSYARNTWLKGSLEI 60
Query: 66 DMFLAFPLSIPVEKLKSKGLEIAESIGKRLESYEISYAEHPYVRGVYKGYQVDIVPCYNV 125
D+FL FP E+L+ +GLEI +++ L+SYEI YAEHPYV GV KG +VD+VPCY +
Sbjct: 61 DVFLLFPEEFSKEELRERGLEIGKAV---LDSYEIRYAEHPYVHGVVKGVEVDVVPCYKL 117
Query: 126 RDWREVRTAVDRSILHTEWVLKNIKGKNDEVRLLKRFLKGINAYGSEVYRRGFSGYLAEI 185
++ + +++AVDR+ H +W+ IKGK +EVRLLK FLK YG+E RGFSGYL E+
Sbjct: 118 KEPKNIKSAVDRTPFHHKWLEGRIKGKENEVRLLKGFLKANGIYGAEYKVRGFSGYLCEL 177
Query: 186 LVIKFGSFLKVLEKADFMLRQKIIDPENWLKREPEIAMKTVKREIEEDKPIIVIDPVDPR 245
L++ +GSFL+ ++ A R+ +ID K E+ + + V+DPVD +
Sbjct: 178 LIVFYGSFLETVKNARRWTRRTVID--------------VAKGEVRKGEEFFVVDPVDEK 223
Query: 246 RNVAANLSWERYGLFYFKAREFLTKPSTELFFPRD----KKGNYLEVLRRKGTHLVTLTF 301
RNVAANLS + F REF+ PS F P+ + +++ +GT + + F
Sbjct: 224 RNVAANLSLDNLARFVHLCREFMEAPSLGFFKPKHPLEIEPERLRKIVEERGTAVFAVKF 283
Query: 302 EPPNLVDDIIIPQVERTAKGLARQLELEGFRVL--GIDYGRDFIFL--EVEEIERPRIKI 357
P++VDD + PQ+ER ++ + LE E F L +F +L E + E R+
Sbjct: 284 RKPDIVDDNLYPQLERASRKIFEFLERENFMPLRSAFKASEEFCYLLFECQIKEISRVFR 343
Query: 358 KKGPLYFTHHGLRFF 372
+ GP + ++ F
Sbjct: 344 RMGPQFEDERNVKKF 358
>sp|Q58511|CCA_METJA TRNA NUCLEOTIDYLTRANSFERASE (TRNA ADENYLYLTRANSFERASE) (TRNA
CCA-PYROPHOSPHORYLASE) (CCA-ADDING ENZYME)
>gi|2826366|gb|AAB99114.1| (U67554) tRNA
nucleotidyltransferase (cca) [Methanococcus jannaschii]
Length = 449
Score = 236 bits (597), Expect = 3e-61
Identities = 156/409 (38%), Positives = 236/409 (57%), Gaps = 17/409 (4%)
Query: 6 LKEVLESIKPKDEERKKVKLIMDELRGIAQEVIEESGEEI-EVKFVGSLAKDTYLSGDHD 64
LKEVL IKP E+ +K++L +E+ E++ E+ I EV VGS A++T L D+D
Sbjct: 10 LKEVLNEIKPSKEDMEKLQLKANEIIDKIWEIVRENSYPILEVLLVGSSARNTNLKDDYD 69
Query: 65 IDMFLAFPLSIPVEKLKSKGLEIAESIGKRLE-SYEISYAEHPYVRGVYKGYQVDIVPCY 123
ID+F+ F S+ ++L+ GL+I KRL SY I+YA HPYV G GY+VDIVPCY
Sbjct: 70 IDIFVLFDKSVSEDELEEIGLKIGTEAIKRLNGSYNINYASHPYVNGEVDGYEVDIVPCY 129
Query: 124 NVRDWREVRTAVDRSILHTEWVLKNIKGKN-DEVRLLKRFLKGINAYGSEVYRRGFSGYL 182
+ ++ +AVDR+ LH ++++ + + DEVRLLK FLK + YGS+V +GFSGYL
Sbjct: 130 KIDFGEKIISAVDRTPLHHKFLISRLNERLCDEVRLLKAFLKSLGLYGSDVKTKGFSGYL 189
Query: 183 AEILVIKFGSFLKVLEKA-DFMLRQKIIDPENWLKREPEIAMKTVKREIEE-DKPIIVID 240
E+L++ +GSF+ +L++A ++ + +KII LK EI ++++ D+P IV D
Sbjct: 190 CELLILHYGSFINLLKEAQNWRIGKKII-----LKDIFEIYKDVDINKLKKFDEPFIVYD 244
Query: 241 PVDPRRNVAANLSWERYGLFYFKAREFLTKPSTELFFPRDKKGNYLEVLRRKGTHLVTLT 300
PVD RNVA+ LS + + F F +R+FL PS E F KK + R G L+ L
Sbjct: 245 PVDLNRNVASPLSKDNFCRFIFYSRQFLKNPSIEFFKDYAKKLEEILENREHGYRLI-LK 303
Query: 301 FEPPNLVDDIIIPQVERTAKGLARQLELEGFRVLG----IDYGRDFIFLEVEEIERPRIK 356
N+VDDII PQ+E+ K + + + F +L D +++ E E P+I
Sbjct: 304 IPRENVVDDIIYPQMEKLQKSINKVIVKNEFVILNSKCFADDNYCYLYWEFLVYELPKIA 363
Query: 357 IKKGPLYF-THHGLRFFDKNDIVWI-EGKELASEKSSLGFIVDVLEDIL 403
+++GP F RF K V+I + K A + I+D+ +DI+
Sbjct: 364 LREGPPVFEKERAERFLKKYGKVFIRDCKLFAYTEREYSHIIDLFKDIV 412
>gb|AAG18757.1| (AE004980) tRNA nucleotidyltransferase; Cca [Halobacterium sp.
NRC-1]
Length = 452
Score = 205 bits (515), Expect = 1e-51
Identities = 125/377 (33%), Positives = 197/377 (52%), Gaps = 25/377 (6%)
Query: 6 LKEVLESIKPKDEERKKVKLIMDELRGIAQEVIEESGEEIEVKFVGSLAKDTYLSGDHDI 65
++ V + + P ER+ + L A+ I E +V VGS A+ T+++GD DI
Sbjct: 8 IERVRQRVDPTPAERRALAAAASRLTERAEAAIAELPVAADVVQVGSTARGTWVAGDRDI 67
Query: 66 DMFLAFPLSIPVEKLKSKGLEIAESIGKRLESYEISYAEHPYVRGVYKGYQVDIVPCYNV 125
D+F+ FP +P E+L++ G + ++ L YAEHPYV G ++GY VD+VPCY+V
Sbjct: 68 DLFVRFPSDLPREQLETYGTTVGAAV---LPDGHEEYAEHPYVTGTFEGYAVDLVPCYDV 124
Query: 126 RDWREVRTAVDRSILHTEWVLKNI-KGKNDEVRLLKRFLKGINAYGSEVYRRGFSGYLAE 184
E+++AVDR+ HT ++ +++ G +VRL K+FLKGI YGS++ +GFSGYL E
Sbjct: 125 AAATEIKSAVDRTPFHTTYLQEHLDDGLAADVRLCKQFLKGIGVYGSDLRTQGFSGYLCE 184
Query: 185 ILVIKFGSFLKVLEKADFMLRQKIIDPENWLKREPEIAMKTVKREIEEDKPIIVIDPVDP 244
+LV+++G F +L + +IDP ++ D P++V+DP DP
Sbjct: 185 LLVVEYGGFEAMLAAIEDWQPPVVIDP-------------AAHQQASFDDPLVVVDPTDP 231
Query: 245 RRNVAANLSWERYGLFYFKAREFLTKPSTELFFPRDKKGNYLEVLR----RKGTHLVTLT 300
RNVAA +S AR F PS + F P LR R+ T + +
Sbjct: 232 ERNVAAVVSAANVATVQHHARRFRATPSEDAFTPASPAPLDAAALRSHIDRRDTTPLAVV 291
Query: 301 FEPPNLVDDIIIPQVERTAKGLARQLELEGFRVLGIDYGRD---FIFLEVEEIERPRIKI 357
+ P++V D + PQ+ R+ G+ R L GF V+ D +F+E+ E P ++
Sbjct: 292 LDAPDVVADQLYPQLYRSRDGVVRGLREHGFDVVRAAAWADERAVVFVELASAELPAVER 351
Query: 358 KKG-PLYFTHHGLRFFD 373
G P+ H RF++
Sbjct: 352 HVGPPVSVGTHAERFYE 368
>emb|CAC12566.1| (AL445067) trna nucleotidyltransferase related protein
[Thermoplasma acidophilum]
Length = 431
Score = 197 bits (496), Expect = 2e-49
Identities = 140/425 (32%), Positives = 214/425 (49%), Gaps = 48/425 (11%)
Query: 7 KEVLESIKPKDEERKKVKLIMDELRGIAQEVIEESGEEIEVKFVGSLAKDTYLSGDHDID 66
+EVL +P EE K+K+I D++ + G E VGS AK T L D D+D
Sbjct: 5 QEVLSRYRPTQEEENKLKIISDDIIRKINSICRSRGLRAEAVIVGSYAKGTNLR-DGDLD 63
Query: 67 MFLAFPLSIPVEKLKSKGLEIAESIGKRLESYEISYAEHPYVRGVYKGYQVDIVPCYNVR 126
+F+AF P E + ++GL I ++ + + YAEHPYV G G ++D+VPCY +
Sbjct: 64 IFIAFDRDYPEEIINTEGLHIGHAV---IPNGREKYAEHPYVSGEIGGVKIDVVPCYKMS 120
Query: 127 DWREVRTAVDRSILHTEWVLKNIKGKN-DEVRLLKRFLKGINAYGSEVYRRGFSGYLAEI 185
+ +AVDR++LHTE+V ++ K DEVRLLK F K I YG+E GFSGYL E+
Sbjct: 121 FGDKKISAVDRTLLHTEYVNGHLDEKGRDEVRLLKIFTKSIGVYGAEARTFGFSGYLCEL 180
Query: 186 LVIKFGSFLKVLEKADFMLRQKIIDPENWLKREPEIAMKTVKREIEEDKPIIVIDPVDPR 245
LVI+FGSF V+ + +ID + + P+++IDPVDP
Sbjct: 181 LVIRFGSFENVIRYFSKAKGRVLIDLDERFR-----------------DPMVLIDPVDPD 223
Query: 246 RNVAANLSWERYGLFYFKAREFLTKPSTELFFPRDKKGNYLEVLRRKGTHLVTLTFEPPN 305
RNVA+ +S E ++ FL+ P E FF + G ++ +GT ++ + P+
Sbjct: 224 RNVASPVSLESLSRMKIASKMFLSSPD-EGFFQIEHNGKNVQ-YHDRGTCIMIYSLPKPD 281
Query: 306 LVDDIIIPQVERTAKGLARQLELEGFRVLGIDY---GRDFIFLEVEEIERPRIKIKKGPL 362
L DD+I PQV R L + +E RV+ + R ++ +E RI++ GP
Sbjct: 282 LTDDVIYPQVYRFRSVLQKIMESHEIRVISSEIDVSDRIYVLIETPACAEERIRVHTGPP 341
Query: 363 YFTHHGLRFFDKNDIVW-----IEGKELASEKSSLGFIVDV------LEDILRKG--QFS 409
T + + F + W G + +++ VDV +EDI+R+ +S
Sbjct: 342 VDTDNAVDFVNS----WKARDRSRGPYIVADR----LYVDVFTGQRSIEDIVRQEIFNYS 393
Query: 410 AGKNV 414
GKN+
Sbjct: 394 IGKNL 398
>gb|AAK41301.1| tRNA nucleotidyltransferase [Sulfolobus solfataricus]
Length = 412
Score = 197 bits (495), Expect = 3e-49
Identities = 131/383 (34%), Positives = 203/383 (52%), Gaps = 35/383 (9%)
Query: 4 MTLKEVLESIKPKDEERKKVKLIMDELRGIAQEVIEESGEEIEVKFVGSLAKDTYLSGDH 63
M +EVL+ IKP +E++K ++ ++ E+I E +++ + GS K T+L D
Sbjct: 1 MIEEEVLKIIKPTEEDKKGIEKVL--------EIIRERLNKLDFEVEGSFRKGTWLRQDT 52
Query: 64 DIDMFLAFPLSIPVEKLKSKGLEIAESIGKRLESYEISYAEHPYVRGVYKGYQVDIVPCY 123
DID+F+ +P + E L+ L + K L+ Y ++YAEHPYV +VDIVP
Sbjct: 53 DIDVFVFYPKDVGKEYLERNALNDIINRIKDLD-YTLAYAEHPYVIVNINNVEVDIVPAL 111
Query: 124 NVRDWREVRTAVDRSILHTEWVLKNI--KGKNDEVRLLKRFLKGINAYGSEVYRRGFSGY 181
V + TAVDR+ HT++V ++ +GK DEVRLLKRF+KGI YG+E+ +GFSGY
Sbjct: 112 RVESGDKAITAVDRTPFHTKYVTSHLDERGK-DEVRLLKRFMKGIGVYGAELKVQGFSGY 170
Query: 182 LAEILVIKFGSFLKVLEKADFMLRQKIIDPENWLKREPEIAMKTVKREIEEDKPIIVIDP 241
E+L+I +G+F KVLE+A K + I ++ K +P+I+ DP
Sbjct: 171 ATELLIIYYGNFRKVLEEAS--------------KWKHPIKIELTKPMKIFSEPLIIPDP 216
Query: 242 VDPRRNVAANLSWERYGLFYFKAREFLTKPSTELFFPRDKKGNYLEVLRRKGTHLVTLTF 301
VDP+RNV A +S + F A+ +L PS E FFP K + KG L+
Sbjct: 217 VDPKRNVTAAVSLKNIATFSIAAKYYLKNPSIEFFFPSKKVEE-----KVKGDVLILRLN 271
Query: 302 EPPNLVDDIIIPQVERTAKGLARQLELEGFRVLGI----DYGRDFIFLEVEEIERPRIKI 357
+DI+ Q++R+ + R L+ GFRV+ + D I +++E + +
Sbjct: 272 LDEKSSEDIVWGQIKRSVNKIERALKQYGFRVIDVQAWGDTNNITIAVQLESKNIGQYYL 331
Query: 358 KKGPLYFTHHGLRFFDKNDIVWI 380
GP Y++ F KND +W+
Sbjct: 332 NIGPQYYSGTIEDFIQKNDNIWV 354
>sp|P77978|CCA_SULSH TRNA NUCLEOTIDYLTRANSFERASE (TRNA ADENYLYLTRANSFERASE) (TRNA
CCA-PYROPHOSPHORYLASE) (CCA-ADDING ENZYME)
>gi|11279001|pir||T48856 tRNA nucleotidyltransferase
[validated] - Sulfolobus shibatae
>gi|1575544|gb|AAB53697.1| (U66004) tRNA
nucleotidyltransferase [Sulfolobus shibatae]
Length = 412
Score = 192 bits (482), Expect = 9e-48
Identities = 132/368 (35%), Positives = 194/368 (51%), Gaps = 35/368 (9%)
Query: 4 MTLKEVLESIKPKDEERKKVKLIMDELRGIAQEVIEESGEEIEVKFVGSLAKDTYLSGDH 63
M +EVL+ IKP +E++K ++ ++ E+I E +++ + GS K T+L D
Sbjct: 1 MIEEEVLKIIKPTEEDKKGIEKVL--------EIIRERLNKLDFEVEGSFRKGTWLRQDT 52
Query: 64 DIDMFLAFPLSIPVEKLKSKGLEIAESIGKRLESYEISYAEHPYVRGVYKGYQVDIVPCY 123
D+D+F+ +P + E L+ L + K L+ Y ++YAEHPYV +VDIVP
Sbjct: 53 DVDVFVFYPKDVGKEYLERNALNDIINRIKDLD-YTLAYAEHPYVIVNINNVEVDIVPAL 111
Query: 124 NVRDWREVRTAVDRSILHTEWVLKNI--KGKNDEVRLLKRFLKGINAYGSEVYRRGFSGY 181
V TAVDR+ HT++V ++ +GK DEVRLLKRF+KGI YG+E+ +GFSGY
Sbjct: 112 RVESGDRAITAVDRTPFHTKYVTSHLDERGK-DEVRLLKRFMKGIGVYGAELKVQGFSGY 170
Query: 182 LAEILVIKFGSFLKVLEKADFMLRQKIIDPENWLKREPEIAMKTVKREIEEDKPIIVIDP 241
E+LVI +GSF KVLE A W K +I + R E P+I+ DP
Sbjct: 171 ATELLVIYYGSFRKVLEAA-----------SKW-KHPIKIELTKPMRAFSE--PLIIPDP 216
Query: 242 VDPRRNVAANLSWERYGLFYFKAREFLTKPSTELFFPRDKKGNYLEVLRRKGTHLVTLTF 301
VDPRRNV A +S + F A+ +L PS E FFP K + KG L+
Sbjct: 217 VDPRRNVTAAVSLKNIATFSVAAKYYLKNPSMEFFFPSKKVEEKI-----KGDVLILRLN 271
Query: 302 EPPNLVDDIIIPQVERTAKGLARQLELEGFRVLGI----DYGRDFIFLEVEEIERPRIKI 357
+DII Q++R+ + R L+ GFRV+ I D I +++E + +
Sbjct: 272 LDEKSSEDIIWGQIKRSVNKIERALKQSGFRVIDIQAWGDTSNIVIAVQLESKNIGQYYL 331
Query: 358 KKGPLYFT 365
GP Y++
Sbjct: 332 NIGPQYYS 339
>pir||F64438 hypothetical protein MJ1111 - Methanococcus jannaschii
Length = 384
Score = 188 bits (472), Expect = 1e-46
Identities = 126/336 (37%), Positives = 191/336 (56%), Gaps = 16/336 (4%)
Query: 78 EKLKSKGLEIAESIGKRLE-SYEISYAEHPYVRGVYKGYQVDIVPCYNVRDWREVRTAVD 136
++L+ GL+I KRL SY I+YA HPYV G GY+VDIVPCY + ++ +AVD
Sbjct: 18 DELEEIGLKIGTEAIKRLNGSYNINYASHPYVNGEVDGYEVDIVPCYKIDFGEKIISAVD 77
Query: 137 RSILHTEWVLKNIKGKN-DEVRLLKRFLKGINAYGSEVYRRGFSGYLAEILVIKFGSFLK 195
R+ LH ++++ + + DEVRLLK FLK + YGS+V +GFSGYL E+L++ +GSF+
Sbjct: 78 RTPLHHKFLISRLNERLCDEVRLLKAFLKSLGLYGSDVKTKGFSGYLCELLILHYGSFIN 137
Query: 196 VLEKA-DFMLRQKIIDPENWLKREPEIAMKTVKREIEE-DKPIIVIDPVDPRRNVAANLS 253
+L++A ++ + +KII LK EI ++++ D+P IV DPVD RNVA+ LS
Sbjct: 138 LLKEAQNWRIGKKII-----LKDIFEIYKDVDINKLKKFDEPFIVYDPVDLNRNVASPLS 192
Query: 254 WERYGLFYFKAREFLTKPSTELFFPRDKKGNYLEVLRRKGTHLVTLTFEPPNLVDDIIIP 313
+ + F F +R+FL PS E F KK + R G L+ L N+VDDII P
Sbjct: 193 KDNFCRFIFYSRQFLKNPSIEFFKDYAKKLEEILENREHGYRLI-LKIPRENVVDDIIYP 251
Query: 314 QVERTAKGLARQLELEGFRVLG----IDYGRDFIFLEVEEIERPRIKIKKGPLYF-THHG 368
Q+E+ K + + + F +L D +++ E E P+I +++GP F
Sbjct: 252 QMEKLQKSINKVIVKNEFVILNSKCFADDNYCYLYWEFLVYELPKIALREGPPVFEKERA 311
Query: 369 LRFFDKNDIVWI-EGKELASEKSSLGFIVDVLEDIL 403
RF K V+I + K A + I+D+ +DI+
Sbjct: 312 ERFLKKYGKVFIRDCKLFAYTEREYSHIIDLFKDIV 347
>pir||T37335 probable tRNA adenylyltransferase (EC 2.7.7.25) - Thermoplasma
acidophilum (fragment) >gi|5689053|dbj|BAA82799.1|
(AB023294) tRNA nucleotidyltransferase [Thermoplasma
acidophilum]
Length = 222
Score = 145 bits (363), Expect = 8e-34
Identities = 88/239 (36%), Positives = 128/239 (52%), Gaps = 22/239 (9%)
Query: 7 KEVLESIKPKDEERKKVKLIMDELRGIAQEVIEESGEEIEVKFVGSLAKDTYLSGDHDID 66
+EVL +P EE ++++I D++ + E VGS AK T L D D+D
Sbjct: 5 QEVLSRYRPTQEEENRLRIISDDIIRKINSICRSRRLRAEAVIVGSYAKGTNLR-DGDLD 63
Query: 67 MFLAFPLSIPVEKLKSKGLEIAESIGKRLESYEISYAEHPYVRGVYKGYQVDIVPCYNVR 126
+F+AF P E + ++GL I ++ + + YAEHPYV G G ++D+VPCY +
Sbjct: 64 IFIAFDRDYPEEIINTEGLHIGHAV---IPNGREKYAEHPYVSGEIGGVKIDVVPCYKMS 120
Query: 127 DWREVRTAVDRSILHTEWVLKNIKGKN-DEVRLLKRFLKGINAYGSEVYRRGFSGYLAEI 185
+AVDR++LHTE+V ++ K DEVRLLK F K I YG+E GFSGYL E+
Sbjct: 121 FGDRKISAVDRTLLHTEYVNGHLDEKGRDEVRLLKIFTKSIGVYGAEARTFGFSGYLCEL 180
Query: 186 LVIKFGSFLKVLEKADFMLRQKIIDPENWLKREPEIAMKTVKREIEEDKPIIVIDPVDP 244
LVI+F SF V+ + +IDP+ + P+++IDPVDP
Sbjct: 181 LVIRFRSFENVIRYFSKAKGRVLIDPDERFR-----------------DPMLLIDPVDP 222
>pir||E72563 probable tRNA nucleotidyltransferase APE1791 - Aeropyrum pernix
(strain K1) >gi|5105481|dbj|BAA80794.1| (AP000062) 435aa
long hypothetical tRNA nucleotidyltransferase [Aeropyrum
pernix]
Length = 435
Score = 125 bits (310), Expect = 1e-27
Identities = 105/329 (31%), Positives = 158/329 (47%), Gaps = 32/329 (9%)
Query: 47 VKFVGSLAKDTYLSGDHDIDMFLAFPLSIPVEKLKSKGLEIAES-IGKRLESYEISYAEH 105
V+ VGS AK T L ++D+FL L P E+++ G + S +G RL Y Y+EH
Sbjct: 28 VELVGSAAKGTLLRDKWEVDVFLL--LDAPREEVRRLGESLLRSCLGGRLPYY-FKYSEH 84
Query: 106 PYVRGVYKGYQVDIVPCYNVRDWREVRTAVDRSILHTEWVLKNIKGKN---DEVRLLKRF 162
PY G Q D+VP D R+ V+R+ HT +V ++ + D++ L K F
Sbjct: 85 PYATVSLMGMQADVVPAPLAVDPRDA-VGVERTPFHTRYVRSRLEERPWLVDDILLFKSF 143
Query: 163 LKGINAYGSEVYRRGFSGYLAEILVIKFGSFLKVLEKADFMLRQKIIDPENWLKREPEIA 222
LKGI YG+E GFSGYLAE+L+I G F + ++ A ++D + +A
Sbjct: 144 LKGIGVYGAETRVGGFSGYLAEVLIIHHGGFEETVKAASSWRPPVMVDTTSGKADLDMLA 203
Query: 223 MKTVKREIEEDKPIIVIDPVDPRRNVAANLSWERYGLFYFKAREFLTKPSTELFFPRDKK 282
+ D PIIV DPVDP RN AA+++ +R A FL KPS FF +
Sbjct: 204 RR------YPDSPIIVPDPVDPSRNTAASVTPKRLAELVHAANIFLRKPS-PTFFHALQP 256
Query: 283 GNYLEVLRRKGTHLVTL-----TFEPPNLVDDIIIPQVERTAKGLARQLELEGFRVLGID 337
G E RR ++ L + PP D + +++R + L R + G+ L
Sbjct: 257 G---EPCRRTLPGVMVLMSGNYSDHPP----DSVWGRLKRLGERLYRATKARGYPALSYS 309
Query: 338 YGRD-----FIFLEVEEIERPRIKIKKGP 361
+ D +++ +E R I+ + GP
Sbjct: 310 FYTDEALEAAVYIHMESPSRYPIEGRLGP 338
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.321 0.142 0.407
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 165676975
Number of Sequences: 2977
Number of extensions: 7226208
Number of successful extensions: 18953
Number of sequences better than 1.0e-10: 12
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 18907
Number of HSP's gapped (non-prelim): 12
length of query: 448
length of database: 189,106,746
effective HSP length: 56
effective length of query: 392
effective length of database: 155,591,474
effective search space: 60991857808
effective search space used: 60991857808
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 166 (69.1 bits)