BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0096 (birA) DE:biotin operon repressor/biotin--[acetyl CoA
carboxylase] ligase (birA)
(237 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||H75202 biotin operon repressor/biotin--[acetyl coa carboxyl... 464 e-130
pir||A71236 probable biotin--[acetyl-CoA-carboxylase] ligase - P... 362 2e-99
gb|AAC25558.1| (AF013169) biotin ligase BirA homolog [Pyrococcus... 326 1e-88
pir||H69122 biotin acetyl-CoA carboxylase ligase / biotin operon... 144 8e-34
sp|P42975|BIRA_BACSU BIRA BIFUNCTIONAL PROTEIN [INCLUDES: BIOTIN... 135 4e-31
gb|AAB60184.1| (U20445) BirA protein [Bacillus subtilis] 132 3e-30
pir||A70351 biotin [acetyl-CoA-carboxylase] ligase - Aquifex aeo... 132 5e-30
pir||C72401 biotin--(acetyl-CoA carboxylase) synthetase - Thermo... 127 2e-28
gi|11497694 biotin operon repressor/biotin--[acetyl CoA carboxyl... 118 4e-26
dbj|BAB05404.1| (AP001512) transcriptional repressor of the biot... 116 2e-25
pir||B64502 bifunctional protein homolog - Methanococcus jannasc... 113 2e-24
gb|AAK40967.1| Biotin--(acetyl-CoA carboxylase) synthetase (bioR... 103 2e-21
emb|CAB57640.1| (Y18930) hypothetical protein [Sulfolobus solfat... 103 2e-21
gb|AAG19158.1| (AE005013) biotin acetyl-CoA carboxylase ligase; ... 101 6e-21
gb|AAA92879.1| (L38424) biotin [acetyl-CoA carboxylase] ligase [... 101 1e-20
dbj|BAA21331.1| (AB005471) ORF105d~similar to BirA bifunctional ... 96 5e-19
pir||D75285 birA bifunctional protein - Deinococcus radiodurans ... 96 5e-19
pir||E82337 birA bifunctional protein VC0319 [imported] - Vibrio... 87 2e-16
gb|AAC49706.1| (U41369) biotin holocarboxylase synthetase [Arabi... 84 1e-15
pir||H70979 probable birA protein - Mycobacterium tuberculosis (... 84 1e-15
gb|AAD31371.1|AC006053_13 (AC006053) biotin holocarboxylase synt... 84 1e-15
pir||A83112 BirA bifunctional protein PA4280 [imported] - Pseudo... 83 2e-15
gb|AAF19230.1|AC007505_6 (AC007505) Putative biotin holocarboxyl... 83 4e-15
sp|P06709|BIRA_ECOLI BIRA BIFUNCTIONAL PROTEIN [INCLUDES: BIOTIN... 81 1e-14
pir||D71657 biotin-protein ligase (birA) RP533 - Rickettsia prow... 80 2e-14
pir||S74680 biotin--[acetyl-CoA-carboxylase] ligase (EC 6.3.4.15... 79 5e-14
gb|AAF33493.1| (AF170176) Salmonella typhimurium bifunctional pr... 77 2e-13
sp|P46363|BIRA_HAEIN BIRA BIFUNCTIONAL PROTEIN [INCLUDES: BIOTIN... 76 3e-13
pir||C71334 probable biotin--acetyl-CoA-carboxylase ligase (birA... 74 1e-12
pir||B81009 BirA protein/Bvg accessory factor NMB2075 [imported]... 73 2e-12
pir||B47751 biotin [acetyl-CoA-carboxylase] ligase homolog - Par... 73 3e-12
sp|P29906|BIRA_PARDE BIOTIN--[ACETYL-COA-CARBOXYLASE] SYNTHETASE... 73 3e-12
pir||H82031 probable BirA bifunctional protein NMA0357 [similari... 71 1e-11
pir||B72026 biotin synthetase - Chlamydophila pneumoniae (strain... 70 3e-11
gb|AAC68833.1| (AF016461) putative biotin protein ligase [Bordet... 69 3e-11
pir||B82637 bifunctional biotin operon/biotin acetyl-CoA-carboxy... 69 6e-11
>pir||H75202 biotin operon repressor/biotin--[acetyl coa carboxylase] ligase
(bira) PAB0096 - Pyrococcus abyssi (strain Orsay)
>gi|5457580|emb|CAB49071.1| (AJ248283) biotin operon
repressor/biotin--[acetyl CoA carboxylase] ligase (birA)
[Pyrococcus abyssi]
Length = 237
Score = 464 bits (1180), Expect = e-130
Identities = 237/237 (100%), Positives = 237/237 (100%)
Query: 1 MLGLKTSVIGRTIIYFQEVASTNDYAKAENLEEGTVIVADRQIKGHGRLERKWESPEGGL 60
MLGLKTSVIGRTIIYFQEVASTNDYAKAENLEEGTVIVADRQIKGHGRLERKWESPEGGL
Sbjct: 1 MLGLKTSVIGRTIIYFQEVASTNDYAKAENLEEGTVIVADRQIKGHGRLERKWESPEGGL 60
Query: 61 WMSVVLTPRVSQEDLPKIVFLGALAVVETLREFSIDARIKWPNDVLVNYRKVAGVLVEAK 120
WMSVVLTPRVSQEDLPKIVFLGALAVVETLREFSIDARIKWPNDVLVNYRKVAGVLVEAK
Sbjct: 61 WMSVVLTPRVSQEDLPKIVFLGALAVVETLREFSIDARIKWPNDVLVNYRKVAGVLVEAK 120
Query: 121 GEKVILGIGLNVNNKVPDGATSMKQELGSEIPMLNVFKTLVKTLDSLYLKFLESPGKILE 180
GEKVILGIGLNVNNKVPDGATSMKQELGSEIPMLNVFKTLVKTLDSLYLKFLESPGKILE
Sbjct: 121 GEKVILGIGLNVNNKVPDGATSMKQELGSEIPMLNVFKTLVKTLDSLYLKFLESPGKILE 180
Query: 181 RAKRSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVSLRFL 237
RAKRSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVSLRFL
Sbjct: 181 RAKRSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVSLRFL 237
>pir||A71236 probable biotin--[acetyl-CoA-carboxylase] ligase - Pyrococcus
horikoshii >gi|3256533|dbj|BAA29216.1| (AP000001) 235aa
long hypothetical biotin--[acetyl-CoA-carboxylase]
ligase [Pyrococcus horikoshii]
Length = 235
Score = 362 bits (919), Expect = 2e-99
Identities = 172/237 (72%), Positives = 207/237 (86%), Gaps = 2/237 (0%)
Query: 1 MLGLKTSVIGRTIIYFQEVASTNDYAKAENLEEGTVIVADRQIKGHGRLERKWESPEGGL 60
MLGLKTS+IGR +IYFQE+ STN++AK LEEGTVIVAD+Q GHGRL RKWESPEGGL
Sbjct: 1 MLGLKTSIIGRRVIYFQEITSTNEFAKTSYLEEGTVIVADKQTMGHGRLNRKWESPEGGL 60
Query: 61 WMSVVLTPRVSQEDLPKIVFLGALAVVETLREFSIDARIKWPNDVLVNYRKVAGVLVEAK 120
W+S+VL+P+V Q+DLPKIVFLGA+ VVETL+EFSID RIKWPNDVLVNY+K+AGVLVE K
Sbjct: 61 WLSIVLSPKVPQKDLPKIVFLGAVGVVETLKEFSIDGRIKWPNDVLVNYKKIAGVLVEGK 120
Query: 121 GEKVILGIGLNVNNKVPDGATSMKQELGSEIPMLNVFKTLVKTLDSLYLKFLESPGKILE 180
G+K++LGIGLNVNNKVP+GATSMK ELGSE+P+L+VF++L+ LD LYL FL++P IL
Sbjct: 121 GDKIVLGIGLNVNNKVPNGATSMKLELGSEVPLLSVFRSLITNLDRLYLNFLKNPMDILN 180
Query: 181 RAKRSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVSLRFL 237
+ +MILGVRVK+L DG EGIAED+D+FGRLI+RLD G VKK++YGDVSLRFL
Sbjct: 181 LVRDNMILGVRVKILGDGSF--EGIAEDIDDFGRLIIRLDSGEVKKVIYGDVSLRFL 235
>gb|AAC25558.1| (AF013169) biotin ligase BirA homolog [Pyrococcus furiosus]
Length = 235
Score = 326 bits (828), Expect = 1e-88
Identities = 155/237 (65%), Positives = 192/237 (80%), Gaps = 2/237 (0%)
Query: 1 MLGLKTSVIGRTIIYFQEVASTNDYAKAENLEEGTVIVADRQIKGHGRLERKWESPEGGL 60
MLGL TS+IG+ +IY++EV STN+ AK N EEGTVIVAD Q GHGR+ R+WESP GGL
Sbjct: 1 MLGLNTSIIGKVVIYYREVTSTNEVAKLGNFEEGTVIVADTQTMGHGRIGRRWESPPGGL 60
Query: 61 WMSVVLTPRVSQEDLPKIVFLGALAVVETLREFSIDARIKWPNDVLVNYRKVAGVLVEAK 120
WMSVVL PR+S EDLPK+ FLGALAVV TL EFSI +IKWPNDVLV+Y+K+AG+LVE +
Sbjct: 61 WMSVVLHPRISTEDLPKLSFLGALAVVRTLSEFSIQGKIKWPNDVLVDYKKIAGILVEKR 120
Query: 121 GEKVILGIGLNVNNKVPDGATSMKQELGSEIPMLNVFKTLVKTLDSLYLKFLESPGKILE 180
G+ V+LG+GLNVNN+ P+ ATSM + LG+ +P++ VF+ LV LD LY FL SP +LE
Sbjct: 121 GDNVVLGVGLNVNNEAPENATSMAEILGTSVPLIEVFRALVTNLDELYKMFLSSPASLLE 180
Query: 181 RAKRSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVSLRFL 237
AK+ MILGV V+++ DGE+ EGIAED+DE GRLI+RL DG +KKI+YGDVSLRFL
Sbjct: 181 MAKKEMILGVPVRIIGDGEI--EGIAEDIDELGRLILRLSDGSLKKIVYGDVSLRFL 235
>pir||H69122 biotin acetyl-CoA carboxylase ligase / biotin operon repressor
bifunctional protein - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2623050|gb|AAB86376.1| (AE000942) biotin acetyl-CoA
carboxylase ligase / biotin operon repressor
bifunctional protein [Methanobacterium
thermoautotrophicum]
Length = 261
Score = 144 bits (360), Expect = 8e-34
Identities = 89/247 (36%), Positives = 135/247 (54%), Gaps = 24/247 (9%)
Query: 6 TSVIGRTIIYFQEVASTNDYAKA---ENLEEGTVIVADRQIKGHGRLERKWESPEGGLWM 62
T IG I F EV STN+ AK + EGTV++A Q +G GR + W SP+GG+WM
Sbjct: 14 TEYIGHEIKCFDEVDSTNNVAKRMAEDGATEGTVVIAKTQSRGRGRRGKPWISPQGGIWM 73
Query: 63 SVVLTPRVSQEDLPKIVFLGALAVVETLR-EFSIDARIKWPNDVLVNYRKVAGVLVEAKG 121
S++L P V P + + +AV TLR E +D IKWPND+L+ +KV G+L EA
Sbjct: 74 SIILRPEVHPSRAPLLTLVAGVAVARTLRDECGLDVGIKWPNDILIGDKKVCGILTEAHA 133
Query: 122 -----EKVILGIGLNVN-------NKVPDGATSMKQELGSEIPMLNVFKTLVKTLDSLYL 169
E V++G+G++ N + + +GATS+K EL +I + ++ + Y
Sbjct: 134 RFNTLEYVVVGVGIDTNVDISHFPDDLREGATSIKNELKRDIKSSELIARFLRNFEETYN 193
Query: 170 KFLESPGK----ILERAKRSMILGVRVKVLSD-GEVEAEGIAEDVDEFGRLIVRLDDGRV 224
F + GK + E K S +G RV++ GE+ G A ++ G LI+ LDDG +
Sbjct: 194 SFKD--GKMDEILTEWRKLSKTIGRRVEIRKQLGEI-VHGDAVGINSEGALILELDDGTL 250
Query: 225 KKILYGD 231
+K++ G+
Sbjct: 251 RKVISGE 257
>sp|P42975|BIRA_BACSU BIRA BIFUNCTIONAL PROTEIN [INCLUDES: BIOTIN OPERON REPRESSOR;
BIOTIN--[ACETYL-COA-CARBOXYLASE] SYNTHETASE
(BIOTIN--PROTEIN LIGASE)] >gi|7450978|pir||A69595
transcription repressor of biotin operon birA - Bacillus
subtilis >gi|1146239|gb|AAB38447.1| (L47709) biotin
[acetyl-CoA-carboxylase] ligase [Bacillus subtilis]
>gi|2634662|emb|CAB14160.1| (Z99115) transcriptional
regulator and biotin acetyl-CoA-carboxylase synthetase
[Bacillus subtilis]
Length = 325
Score = 135 bits (337), Expect = 4e-31
Identities = 87/253 (34%), Positives = 139/253 (54%), Gaps = 25/253 (9%)
Query: 3 GLKTSVIGRTIIYFQEVASTNDYAKA---ENLEEGTVIVADRQIKGHGRLERKWESPEG- 58
GLKT V+G+ +IY ++ST A N EGT++VAD+Q G GR+ R W S EG
Sbjct: 76 GLKTEVMGQHLIYHDVLSSTQKTAHELANNNAPEGTLVVADKQTAGRGRMSRVWHSQEGN 135
Query: 59 GLWMSVVLTPRVSQEDLPKIVFLGALAVVETLREFS-IDARIKWPNDVLVNYRKVAGVLV 117
G+WMS++L P + + P++ L A+AVV+ + E + I IKWPND+L+N +K G+L
Sbjct: 136 GVWMSLILRPDIPLQKTPQLTLLAAVAVVQGIEEAAGIQTDIKWPNDILINGKKTVGILT 195
Query: 118 EAKGEK-----VILGIGLNVN-------NKVPDGATSMKQELGSEIPMLNVFKTLVKTLD 165
E + E+ VI+GIG+NVN +++ D ATS+ Q G +I V + ++ +
Sbjct: 196 EMQAEEDRVRSVIIGIGINVNQQPNDFPDELKDIATSLSQAAGEKIDRAGVIQHILLCFE 255
Query: 166 SLYLKFLESPGKILERAKRSMILGV----RVKVLSDGEVEAEGIAEDVDEFGRLIVRLDD 221
Y ++ ++ S LG+ R + L+ G A +D+ G L++ ++
Sbjct: 256 KRYRDYMTHGFTPIKLLWESYALGIGTNMRARTLNG---TFYGKALGIDDEGVLLLETNE 312
Query: 222 GRVKKILYGDVSL 234
G +KKI D+ L
Sbjct: 313 G-IKKIYSADIEL 324
>gb|AAB60184.1| (U20445) BirA protein [Bacillus subtilis]
Length = 325
Score = 132 bits (330), Expect = 3e-30
Identities = 86/253 (33%), Positives = 137/253 (53%), Gaps = 25/253 (9%)
Query: 3 GLKTSVIGRTIIYFQEVASTNDYAKA---ENLEEGTVIVADRQIKGHGRLERKWESPEG- 58
GLKT V+G+ +IY ++ST A N EGT++VAD+Q G GR+ R W S EG
Sbjct: 76 GLKTEVMGQHLIYQDVISSTQKTAHELANNNAPEGTLVVADKQTAGRGRMSRVWHSQEGN 135
Query: 59 GLWMSVVLTPRVSQEDLPKIVFLGALAVVETLREFS-IDARIKWPNDVLVNYRKVAGVLV 117
G+WMS++L P + + P++ L A+AVV+ + + I IKWPND+L+N +K G+L
Sbjct: 136 GIWMSLILRPDIPLQKTPQLTLLAAVAVVQGIEAAAGIQTDIKWPNDILINGKKTVGILT 195
Query: 118 EAKGEK-----VILGIGLNVN-------NKVPDGATSMKQELGSEIPMLNVFKTLVKTLD 165
E + E+ VI+GIG+NVN +++ D ATS+ Q G +I V + ++ +
Sbjct: 196 EMQAEEDRVRSVIIGIGINVNQQSDDFPDELKDIATSLSQASGEKIDRAGVIQHILLCFE 255
Query: 166 SLYLKFLESPGKILERAKRSMILGV----RVKVLSDGEVEAEGIAEDVDEFGRLIVRLDD 221
Y ++ ++ S LG+ R + L+ G A +D+ G L++ +
Sbjct: 256 KRYRDYMTHGFTPIKLLWESYALGIGTNMRARTLNG---TFYGKALGIDDEGVLLLETQE 312
Query: 222 GRVKKILYGDVSL 234
G +KKI D+ L
Sbjct: 313 G-IKKIYSADIEL 324
>pir||A70351 biotin [acetyl-CoA-carboxylase] ligase - Aquifex aeolicus
>gi|2983199|gb|AAC06798.1| (AE000696) biotin
[acetyl-CoA-carboxylase] ligase [Aquifex aeolicus]
Length = 233
Score = 132 bits (328), Expect = 5e-30
Identities = 88/236 (37%), Positives = 136/236 (57%), Gaps = 18/236 (7%)
Query: 11 RTIIYFQEVASTNDYAKAENLEEGTVIVADRQIKGHGRLERKWESPEGGLWMSVVLTPRV 70
+ +I+ +EV ST + K N+ GT +VADRQ KG GRL RKW S EGGL+ S +L P+
Sbjct: 3 KNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGRLGRKWLSQEGGLYFSFLLNPK- 61
Query: 71 SQEDLPKIVFLGALAVVETLREFS-IDARIKWPNDVLVNYRKVAGVLVEAKGEKVILGIG 129
E+L ++ + L+V E L E + I +KWPNDV +KV+GVL E +K+I+GIG
Sbjct: 62 EFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVSGVLCELSKDKLIVGIG 121
Query: 130 LNVNNK-----VPDGATSMKQELGSEIP----MLNVFKTLVKTLDSLYLK-FLESPGKIL 179
+NVN + + D AT++ + G + +L V K + + L K F E GKI
Sbjct: 122 INVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISENLKKFKEKSFKEFKGKI- 180
Query: 180 ERAKRSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVSLR 235
+ + LG VK+L +G++ + + + E G ++ ++G +K+IL G+ SLR
Sbjct: 181 --ESKMLYLGEEVKLLGEGKITGKLVG--LSEKGGALILTEEG-IKEILSGEFSLR 231
>pir||C72401 biotin--(acetyl-CoA carboxylase) synthetase - Thermotoga maritima
(strain MSB8) >gi|4980723|gb|AAD35316.1|AE001707_3
(AE001707) biotin--(acetyl-CoA carboxylase) synthetase
[Thermotoga maritima]
Length = 231
Score = 127 bits (315), Expect = 2e-28
Identities = 76/192 (39%), Positives = 113/192 (58%), Gaps = 14/192 (7%)
Query: 8 VIGRTIIYFQEVASTNDYAKAENLE---EGTVIVADRQIKGHGRLERKWESPEGGLWMSV 64
+IG II F+ + STN + K EN +GTV+VA Q G+GR R W SP+GGLW SV
Sbjct: 1 MIGEKIISFESIDSTNRFLK-ENYRFYPDGTVVVALEQTSGYGRSGRHWHSPKGGLWFSV 59
Query: 65 VLTPRVSQEDLPKIVFLGALAVVETLREFSIDARIKWPNDVLVNYRKVAGVLVE-----A 119
+ PR E L + ++AVV+TL I A IKWPNDV +N RK+AGVL E A
Sbjct: 60 LFKPRKPLE-LSFYTRVFSVAVVKTLENMKIHANIKWPNDVYINGRKLAGVLSEGIFEGA 118
Query: 120 KGEKVILGIGLNVNNKVP----DGATSMKQELGSEIPMLNVFKTLVKTLDSLYLKFLESP 175
K V++G+G+NVNN++P A S+K+ +G EI ++ + ++++K ++ K+
Sbjct: 119 KPLAVVVGVGMNVNNEIPAELKTRAISLKEIMGKEISIVKLMESMLKNARVIFRKYSRKK 178
Query: 176 GKILERAKRSMI 187
+ KR ++
Sbjct: 179 EALTRIWKRYLL 190
>gi|11497694 biotin operon repressor/biotin--[acetyl CoA carboxylase] ligase
(birA) [Archaeoglobus fulgidus] >gi|7450984|pir||B69259
biotin operon repressor/biotin--[acetyl CoA carboxylase]
ligase (birA) homolog - Archaeoglobus fulgidus
>gi|2650573|gb|AAB91153.1| (AE001101) biotin operon
repressor/biotin--[acetyl CoA carboxylase] ligase (birA)
[Archaeoglobus fulgidus]
Length = 299
Score = 118 bits (294), Expect = 4e-26
Identities = 83/225 (36%), Positives = 120/225 (52%), Gaps = 20/225 (8%)
Query: 14 IYFQEVA-STNDYAKAENLEEGTVIVADRQIKGHGRLERKWESPEGGLWMSVVLTPRVSQ 72
++F +V STN AK E + + A+RQ G GR RKW S EGGL+ SV L+P +
Sbjct: 83 VHFYDVTDSTNSRAK-EYGKPDALFFANRQTAGRGRHGRKWLSEEGGLYFSVTLSPPLDY 141
Query: 73 EDLPKIVFLGALAVVETLREFSIDARIKWPNDVLVNYRKVAGVLVEAKGE----KVILGI 128
+LPK+ + L+V E + + + IKWPNDVL+ RKV G+L E GE VI+G+
Sbjct: 142 SELPKLTLIAGLSVAEAIPQ----SEIKWPNDVLIKGRKVCGILSELHGEVERPLVIVGV 197
Query: 129 GLNVNNKVPDGATSMKQELGSEIPMLNVFKTLVKTLDSLYLKFLESPGKILER--AKRSM 186
G+NV N P+ S+ + ++ VF+ +++ Y L+ L R +R
Sbjct: 198 GINVKNPHPENGISLSELY--DVSRREVFEQVIRNFSKNYRMLLDGRWCELRRRIERRCS 255
Query: 187 ILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGD 231
+G V+V + V AEGIAE + E G L+V KKI GD
Sbjct: 256 SVGKAVRVTTPSGV-AEGIAEAISEDGSLVV-----SGKKIYAGD 294
>dbj|BAB05404.1| (AP001512) transcriptional repressor of the biotin operon [Bacillus
halodurans]
Length = 323
Score = 116 bits (288), Expect = 2e-25
Identities = 77/251 (30%), Positives = 138/251 (54%), Gaps = 22/251 (8%)
Query: 4 LKTSVIGRTIIYFQEVASTNDYA---KAENLEEGTVIVADRQIKGHGRLERKWESPEGG- 59
L+T GR I Y + AST A E +EG +++A+ Q G GR+ R W SP G
Sbjct: 73 LETERFGREITYLESTASTQTVALKLAQEGAKEGHIVLANEQTSGKGRMGRGWYSPPGSS 132
Query: 60 LWMSVVLTPRVSQEDLPKIVFLGALAVVETLREFS-IDARIKWPNDVLVNYRKVAGVLVE 118
+ MS++ P++ + P++ L A+A+V ++E + +D+ IKWPND+L++ +K+ G+L E
Sbjct: 133 ISMSIIFRPQLPPQKAPQLTLLTAVAIVRAIKETTGLDSDIKWPNDLLIDGKKIVGILTE 192
Query: 119 AKGEK-----VILGIGLNVNN-------KVPDGATSMKQELGSEIPMLNVFKTLVKTLD- 165
+ ++ VI GIG+NVN+ ++ ATS+ + G I + ++K ++
Sbjct: 193 MQADQDSVHSVIQGIGINVNHQEEAFAEEIRKIATSLAIKKGEPIQRAPLIAAILKNIEL 252
Query: 166 --SLYLKFLESPGKILERAKRSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGR 223
LYL+ S K L A ++ +G R++ +V+ G+A+ + + G L++ DDG+
Sbjct: 253 FYDLYLQHGFSRIKPLWEA-HAISIGKRIRARMLNDVKF-GVAKGITDDGVLLLEDDDGK 310
Query: 224 VKKILYGDVSL 234
+ I D+ +
Sbjct: 311 LHSIYSADIEI 321
>pir||B64502 bifunctional protein homolog - Methanococcus jannaschii
>gi|1592217|gb|AAB99640.1| (U67602) biotin operon
repressor/biotin--[acetyl-CoA-carboxylase] ligase (birA)
[Methanococcus jannaschii]
Length = 237
Score = 113 bits (280), Expect = 2e-24
Identities = 75/227 (33%), Positives = 132/227 (58%), Gaps = 22/227 (9%)
Query: 13 IIYFQEVASTNDYAK--AENLEEGTVIVADRQIKGHGRLERKWESPEGGLWMSVVLTPRV 70
II+ E+ STNDYAK A+ + +++AD+Q G GR R W S EGGL+ S+VL ++
Sbjct: 3 IIHLSEIDSTNDYAKELAKEGKRNFIVLADKQNNGKGRWGRVWYSDEGGLYFSMVLDSKL 62
Query: 71 SQEDLPKIV-FLGALAVVETLREF-SIDARIKWPNDVLV----NYRKVAGVLVEAKGEKV 124
PK++ L + ++E L+ + + +K+PND++V NY+K+ G+L E + +
Sbjct: 63 YN---PKVINLLVPICIIEVLKNYVDKELGLKFPNDIMVKVNDNYKKLGGILTELTDDYM 119
Query: 125 ILGIGLNVNNKVPDG----ATSMKQELGSEIPMLNVFKTLVKTLDSLYLKFLESPG---- 176
I+GIG+NVNN++ + A S+K+ G E+ + + +KT +S YL+ L++
Sbjct: 120 IIGIGINVNNQIRNEIREIAISLKEITGKELDKVEILSNFLKTFES-YLEKLKNKEIDDY 178
Query: 177 KILERAKR-SMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDG 222
+IL++ K+ S+ +G +VK+L G D+D F +++ + G
Sbjct: 179 EILKKYKKYSITIGKQVKILLSNNEIITGKVYDID-FDGIVLGTEKG 224
>gb|AAK40967.1| Biotin--(acetyl-CoA carboxylase) synthetase (bioR) [Sulfolobus
solfataricus]
Length = 233
Score = 103 bits (254), Expect = 2e-21
Identities = 77/233 (33%), Positives = 125/233 (53%), Gaps = 19/233 (8%)
Query: 13 IIYFQEVASTNDYAKAEN--LEEGTVIVADRQIKGHGRLERKWESPEGGLWMSVVLTPRV 70
+I +V ST D+A+A + L E +++A+ Q K GRL R W SP+GGLW + V
Sbjct: 7 VIRLNKVTSTQDFAEAISNMLFEDFLVIAEEQTKARGRLGRSWYSPKGGLWFTYV-KKNF 65
Query: 71 SQEDLPKIVFLGALAVVETLREFSIDARIKWPNDVLVNYRKVAGVLVEAKGEK------- 123
E++ A+AV++ LR+F +DA+I+WPND++VN +K+AG+L+EAK +
Sbjct: 66 KTEEIQLSSLKVAVAVLDVLRKF-VDAKIRWPNDIVVNDKKIAGILIEAKTYEEDIKLAD 124
Query: 124 VILGIGLNVNNK-VPD--GATSMKQELGSEIPMLNVFKTLVKTLDSLYLKFLESPGKILE 180
+ +G G+++ K P ATS+ E+G E+ LN+ + + K + L L KI++
Sbjct: 125 LFIGAGIDILIKEFPSDLNATSLYLEIGKEVE-LNIDEVIGKIEEHLKL----DNRKIID 179
Query: 181 RAKRSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVS 233
+ + + R L E A VD GRL+ V++I +VS
Sbjct: 180 IVNQYLSIKDREVTLKGENWEKRCKALFVDYMGRLVTECGIYEVEEIYRVEVS 232
>emb|CAB57640.1| (Y18930) hypothetical protein [Sulfolobus solfataricus]
Length = 233
Score = 103 bits (254), Expect = 2e-21
Identities = 77/233 (33%), Positives = 125/233 (53%), Gaps = 19/233 (8%)
Query: 13 IIYFQEVASTNDYAKAEN--LEEGTVIVADRQIKGHGRLERKWESPEGGLWMSVVLTPRV 70
+I +V ST D+A+A + L E +++A+ Q K GRL R W SP+GGLW + V
Sbjct: 7 VIRLNKVTSTQDFAEAISNMLFEDFLVIAEEQTKARGRLGRSWYSPKGGLWFTYV-KKNF 65
Query: 71 SQEDLPKIVFLGALAVVETLREFSIDARIKWPNDVLVNYRKVAGVLVEAKGEK------- 123
E++ A+AV++ LR+F +DA+I+WPND++VN +K+AG+L+EAK +
Sbjct: 66 KTEEIQLSSLKVAVAVLDVLRKF-VDAKIRWPNDIVVNDKKIAGILIEAKTYEEDIKLAD 124
Query: 124 VILGIGLNVNNK-VPD--GATSMKQELGSEIPMLNVFKTLVKTLDSLYLKFLESPGKILE 180
+ +G G+++ K P ATS+ E+G E+ LN+ + + K + L L KI++
Sbjct: 125 LFIGAGIDILIKEFPSDLNATSLYLEIGKEVE-LNIDEVIGKIEEHLKL----DNRKIID 179
Query: 181 RAKRSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVS 233
+ + + R L E A VD GRL+ V++I +VS
Sbjct: 180 IVNQYLSIKDREVTLKGENWEKRCKALFVDYMGRLVTECGIYEVEEIYRVEVS 232
>gb|AAG19158.1| (AE005013) biotin acetyl-CoA carboxylase ligase; BirL
[Halobacterium sp. NRC-1]
Length = 282
Score = 101 bits (250), Expect = 6e-21
Identities = 74/256 (28%), Positives = 116/256 (44%), Gaps = 43/256 (16%)
Query: 9 IGRTIIYFQEVASTNDYAKAE---NLEEGTVIVADRQIKGHGRLERKWESPEGGLWMSVV 65
+ +++ + STND A+AE +GT ++AD Q G GR W SP GG+W S V
Sbjct: 12 VSAPVVHRETCPSTNDIARAEARAGATDGTFVIADEQTAGRGRTGNAWASPPGGVWSSTV 71
Query: 66 LTPRVSQEDLPKIVFLGALAVVETLREFSIDARIKWPNDVLV------NYRKVAGVLVEA 119
+ ++ + ++ G LA +T F +DAR+KWPNDV+V + K+ GVL EA
Sbjct: 72 VRADIAASHVGRLTLAGGLAAADTAASFGVDARLKWPNDVVVAAADGSDQSKLCGVLTEA 131
Query: 120 KGEKV------------------------ILGIGLNVN------NKVPDGATSMKQELGS 149
++V +LGIG+N + T+++ E G+
Sbjct: 132 VIDEVPVAGKPVGSVLPETDPANADMSFAVLGIGVNAALDPDSLGDLDRAVTTLRAERGA 191
Query: 150 EIPMLNVFKTLVKTLDSLYLKFLESPG---KILERAKRSMILGVRVKVLSDGEVEAEGIA 206
P V TL + L + + + G + +RS LG RV+V + G A
Sbjct: 192 VDPTA-VAATLHEHLRTWVSRVQTAGGMDDAVAAVRERSATLGKRVRVTRFDDDPVTGTA 250
Query: 207 EDVDEFGRLIVRLDDG 222
+D+ G L+V DG
Sbjct: 251 VAIDDRGALVVERPDG 266
>gb|AAA92879.1| (L38424) biotin [acetyl-CoA carboxylase] ligase [Bacillus subtilis]
Length = 305
Score = 101 bits (248), Expect = 1e-20
Identities = 77/253 (30%), Positives = 126/253 (49%), Gaps = 45/253 (17%)
Query: 3 GLKTSVIGRTIIYFQEVASTNDYAKA---ENLEEGTVIVADRQIKGHGRLERKWESPEG- 58
GLKT V+G+ +IY ++ST A N EGT+ EG
Sbjct: 76 GLKTEVMGQHLIYHDVLSSTQKTAHELANNNAPEGTL--------------------EGN 115
Query: 59 GLWMSVVLTPRVSQEDLPKIVFLGALAVVETLREFS-IDARIKWPNDVLVNYRKVAGVLV 117
G+WMS++L P + + P++ L A+AVV+ + E + I IKWPND+L+N +K G+L
Sbjct: 116 GVWMSLILRPDIPLQKTPQLTLLAAVAVVQGIEEAAGIQTDIKWPNDILINGKKTVGILT 175
Query: 118 EAKGEK-----VILGIGLNVN-------NKVPDGATSMKQELGSEIPMLNVFKTLVKTLD 165
E + E+ VI+GIG+NVN +++ D ATS+ Q G +I V + ++ +
Sbjct: 176 EMQAEEDRVRSVIIGIGINVNQQPNDFPDELKDIATSLSQAAGEKIDRAGVIQHILLCFE 235
Query: 166 SLYLKFLESPGKILERAKRSMILGV----RVKVLSDGEVEAEGIAEDVDEFGRLIVRLDD 221
Y ++ ++ S LG+ R + L+ G A +D+ G L++ ++
Sbjct: 236 KRYRDYMTHGFTPIKLLWESYALGIGTNMRARTLNG---TFYGKALGIDDEGVLLLETNE 292
Query: 222 GRVKKILYGDVSL 234
G +KKI D+ L
Sbjct: 293 G-IKKIYSADIEL 304
>dbj|BAA21331.1| (AB005471) ORF105d~similar to BirA bifunctional proteins
[Deinococcus radiodurans]
Length = 322
Score = 95.6 bits (234), Expect = 5e-19
Identities = 80/238 (33%), Positives = 118/238 (48%), Gaps = 20/238 (8%)
Query: 10 GRTIIYFQEVASTND----YAKAEN--LEEGTVIVADRQIKGHGRLERKWESPEGGLWMS 63
GR + Y V ST D +A +E G V+VA+RQ G GR R W++ G L S
Sbjct: 86 GRALRYQGTVTSTQDEVRFWADSERGPAPHGAVVVAERQTAGRGRRGRVWDTTHGTLAFS 145
Query: 64 VVLTPRVSQEDLPKIVFLGALAVVETLREFSIDARIKWPNDVLV-NYRKVAGVLVEA--K 120
V+L +S DL ++ +A+ + +KWPND+L + RK+AG+L+EA +
Sbjct: 146 VLLREALSLADLVRMPLAAGVALHAACTALGVPCGLKWPNDLLTPDGRKLAGILLEADLR 205
Query: 121 GE---KVILGIGLNVNNKVPDGATSMKQELGSEIPMLNVFKTLVKTLDSL-YLKFLESPG 176
GE + +LGIG+NV P+GA L +P L L + L L + +P
Sbjct: 206 GEEARRAVLGIGINVGT-APEGAA----HLHESVPDLTRAAVLGEVLFQLEHWLAQPAPA 260
Query: 177 KILERAKRSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVSL 234
I S+ LG V+V + EG+A D+DE G L+V G ++ + GDV L
Sbjct: 261 IIATWKATSLTLGRPVRV-TTARGTVEGVARDLDEQGNLLVETAAG-LETVSAGDVQL 316
>pir||D75285 birA bifunctional protein - Deinococcus radiodurans (strain R1)
>gi|6460154|gb|AAF11888.1|AE002065_5 (AE002065) birA
bifunctional protein [Deinococcus radiodurans]
Length = 474
Score = 95.6 bits (234), Expect = 5e-19
Identities = 80/238 (33%), Positives = 118/238 (48%), Gaps = 20/238 (8%)
Query: 10 GRTIIYFQEVASTND----YAKAEN--LEEGTVIVADRQIKGHGRLERKWESPEGGLWMS 63
GR + Y V ST D +A +E G V+VA+RQ G GR R W++ G L S
Sbjct: 238 GRALRYQGTVTSTQDEVRFWADSERGPAPHGAVVVAERQTAGRGRRGRVWDTTHGTLAFS 297
Query: 64 VVLTPRVSQEDLPKIVFLGALAVVETLREFSIDARIKWPNDVLV-NYRKVAGVLVEA--K 120
V+L +S DL ++ +A+ + +KWPND+L + RK+AG+L+EA +
Sbjct: 298 VLLREALSLADLVRMPLAAGVALHAACTALGVPCGLKWPNDLLTPDGRKLAGILLEADLR 357
Query: 121 GE---KVILGIGLNVNNKVPDGATSMKQELGSEIPMLNVFKTLVKTLDSL-YLKFLESPG 176
GE + +LGIG+NV P+GA L +P L L + L L + +P
Sbjct: 358 GEEARRAVLGIGINVGT-APEGAA----HLHESVPDLTRAAVLGEVLFQLEHWLAQPAPA 412
Query: 177 KILERAKRSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVSL 234
I S+ LG V+V + EG+A D+DE G L+V G ++ + GDV L
Sbjct: 413 IIATWKATSLTLGRPVRV-TTARGTVEGVARDLDEQGNLLVETAAG-LETVSAGDVQL 468
>pir||E82337 birA bifunctional protein VC0319 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9654732|gb|AAF93492.1|
(AE004120) birA bifunctional protein [Vibrio cholerae]
Length = 320
Score = 86.6 bits (211), Expect = 2e-16
Identities = 69/234 (29%), Positives = 115/234 (48%), Gaps = 19/234 (8%)
Query: 19 VASTNDYA--KAENLEEGTVIVADRQIKGHGRLERKWESPEGG-LWMSVVLTPRVSQEDL 75
+ STN Y E L GTV +A+ Q G GR R W SP G L++S+
Sbjct: 86 IGSTNQYLLDHVETLVSGTVCLAEYQASGRGRRGRHWVSPFGANLYLSIYWRLDAGMAAA 145
Query: 76 PKIVFLGALAVVETLREFSIDA-RIKWPNDVLVNYRKVAGVLVEAKGE-----KVILGIG 129
+ + +A+VE L +D ++KWPND+ +K+AG+LVE G+ +++G+G
Sbjct: 146 MGLSLVVGVAIVEALEAMGVDGVKLKWPNDLYYQDKKLAGILVEMSGQAGAAAHLVIGMG 205
Query: 130 LNVNNKVPDGA-----TSMKQELG-SEIPMLNVFKTLVKTLDSLYLKF-LESPGKILERA 182
+N+ + +G S+ + G S I + L+ LD ++ + +ER
Sbjct: 206 INLAMRDNEGNIDQPWISLAEVTGQSRIDRNALAINLIAALDRTLRQYEISGMQNFVERW 265
Query: 183 KR-SMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVSLR 235
R +G VK+L G E GI +DE G +++ ++G +K + G++SLR
Sbjct: 266 NRWDNFIGRPVKLLM-GANEVRGIERGIDEHGGVLLETEEG-LKSFIGGEISLR 317
>gb|AAC49706.1| (U41369) biotin holocarboxylase synthetase [Arabidopsis thaliana]
Length = 367
Score = 84.3 bits (205), Expect = 1e-15
Identities = 70/223 (31%), Positives = 103/223 (45%), Gaps = 28/223 (12%)
Query: 1 MLGLKTSVIGRTIIYFQEVASTNDYAKAE--NLEEGTVIVADRQIKGHGRLERKWESPEG 58
M + T GR +I+ ++ST+D L G+V V D Q KG GR + WESP+G
Sbjct: 102 MNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVTDIQFKGRGRTKNVWESPKG 161
Query: 59 GLWMSVVLTPRVSQED---LPKIVFLGALAVVETLREFS-------IDARIKWPNDVLVN 108
L S L ED +P I ++ +LAV E +++ ID +IKWPND+ VN
Sbjct: 162 CLMYSFTL----EMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPNDLYVN 217
Query: 109 YRKVAGVL----VEAKGEKVILGIGLNVNNKVPDGATSMKQELGSEIPMLNVFK------ 158
KV G+L +K V +G+GLNV+N P T + L P N+ K
Sbjct: 218 GLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQP--TTCLNAVLKGMAPESNLLKREEILG 275
Query: 159 TLVKTLDSLYLKFLESPGKILERAKRSMILGVRVKVLSDGEVE 201
+ + F++ K LE L +V+ + +VE
Sbjct: 276 AFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVE 318
>pir||H70979 probable birA protein - Mycobacterium tuberculosis (strain H37RV)
>gi|1877334|emb|CAB07082.1| (Z92771) birA [Mycobacterium
tuberculosis]
Length = 266
Score = 84.3 bits (205), Expect = 1e-15
Identities = 66/214 (30%), Positives = 108/214 (49%), Gaps = 19/214 (8%)
Query: 33 EGTVIVADRQIKGHGRLERKWESPEGGLWMSVVLTPRVSQEDLPK-----IVFLGALAVV 87
+G V++A+ Q G GR R W + ++L+ V D+P + LAV+
Sbjct: 54 DGVVLIAEHQTAGRGRHGRGWAATARA---QIILSVGVRVVDVPVQAWGWLSLAAGLAVL 110
Query: 88 ETLREF----SIDARIKWPNDVLVNYRKVAGVLVEAKGEKVILGIGLNVN---NKVPDGA 140
+++ + +KWPNDVL K+AG+L E V+LG+GLNV +V A
Sbjct: 111 DSVAPLIAVPPAETGLKWPNDVLARGGKLAGILAEVAQPFVVLGVGLNVTQAPEEVDPDA 170
Query: 141 TSMKQELGSEIPMLN-VFKTLVKTLDSLYLKFLE-SPGKILERAKRSMILGVRVKVLSDG 198
TS+ +LG P N + L++ L++ +++ +P + RS+ +G RV+V G
Sbjct: 171 TSL-LDLGVAAPDRNRIASRLLRELEARIIQWRNANPQLAADYRARSLTIGSRVRVELPG 229
Query: 199 EVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDV 232
+ GIA D+D+ GRL + + GR + GDV
Sbjct: 230 GQDVVGIARDIDDQGRLCLDV-GGRTVVVSAGDV 262
>gb|AAD31371.1|AC006053_13 (AC006053) biotin holocarboxylase synthetase [Arabidopsis thaliana]
Length = 355
Score = 84.3 bits (205), Expect = 1e-15
Identities = 70/223 (31%), Positives = 103/223 (45%), Gaps = 28/223 (12%)
Query: 1 MLGLKTSVIGRTIIYFQEVASTNDYAKAE--NLEEGTVIVADRQIKGHGRLERKWESPEG 58
M + T GR +I+ ++ST+D L G+V V D Q KG GR + WESP+G
Sbjct: 102 MNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVTDIQFKGRGRTKNVWESPKG 161
Query: 59 GLWMSVVLTPRVSQED---LPKIVFLGALAVVETLREFS-------IDARIKWPNDVLVN 108
L S L ED +P I ++ +LAV E +++ ID +IKWPND+ VN
Sbjct: 162 CLMYSFTL----EMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPNDLYVN 217
Query: 109 YRKVAGVL----VEAKGEKVILGIGLNVNNKVPDGATSMKQELGSEIPMLNVFK------ 158
KV G+L +K V +G+GLNV+N P T + L P N+ K
Sbjct: 218 GLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQP--TTCLNAVLKGMAPESNLLKREEILG 275
Query: 159 TLVKTLDSLYLKFLESPGKILERAKRSMILGVRVKVLSDGEVE 201
+ + F++ K LE L +V+ + +VE
Sbjct: 276 AFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVE 318
>pir||A83112 BirA bifunctional protein PA4280 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9950499|gb|AAG07668.1|AE004843_10 (AE004843) BirA
bifunctional protein [Pseudomonas aeruginosa]
Length = 312
Score = 83.5 bits (203), Expect = 2e-15
Identities = 75/234 (32%), Positives = 112/234 (47%), Gaps = 20/234 (8%)
Query: 19 VASTNDYAK---AENLEEGTVIVADRQIKGHGRLERKWESPEG-GLWMSVVLTPRVSQED 74
V STN A A VIVA++Q G GR R W SP G L+ S+ +T R +
Sbjct: 79 VDSTNSEAMRRLAAGASTPFVIVAEQQTAGRGRRGRAWVSPYGENLYYSLAITVRGGARE 138
Query: 75 LPKIVFLGALAVVETLREFSI-DARIKWPNDVLVNYRKVAGVLVEAKGE-----KVILGI 128
L + + LAV+ L + D +KWPND+L +K+AG+L+E G+ V++GI
Sbjct: 139 LEGLSLVVGLAVLRALESLGVQDTGLKWPNDLLQGGKKIAGILLELSGDPADLCHVVIGI 198
Query: 129 GLNVNNK----VPDGATSMKQELGSEIPMLNVFKTLVKTLDSLYLKFLESPGKILERAK- 183
G+NVN K + TS+++ LG + + L + L YL G R +
Sbjct: 199 GINVNMKTATDIGQPWTSLREALGELVDRSRLLSALNRQLAD-YLDRHSREGFAASREEW 257
Query: 184 --RSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVSLR 235
++ G +V +LS EG VD G L + + DG + G++SLR
Sbjct: 258 ESCNLWQGAQV-ILSTALSSIEGRVLGVDSRGALRLEV-DGVEQGFSGGELSLR 309
>gb|AAF19230.1|AC007505_6 (AC007505) Putative biotin holocarboxylase synthetase [Arabidopsis
thaliana]
Length = 310
Score = 82.7 bits (201), Expect = 4e-15
Identities = 65/203 (32%), Positives = 101/203 (49%), Gaps = 30/203 (14%)
Query: 1 MLGLKTSVIGRTIIYFQEVASTNDYAKAENLEE---GTVIVADRQIKGHGRLERKWESPE 57
M + T GR +I+ ++ST+D + N E G+V V+D Q+KG GR + WESP+
Sbjct: 64 MNSISTHRFGRFLIWSPYLSSTHDVV-SHNFSEIPVGSVCVSDIQLKGRGRTKNVWESPK 122
Query: 58 GGLWMSVVLTPRVSQED---LPKIVFLGALAVVETLREFS-------IDARIKWPNDVLV 107
G L S L ED +P I ++ +LAV E +++ D +IKWPND+ +
Sbjct: 123 GCLMYSFTL----EMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYL 178
Query: 108 NYRKVAGVL----VEAKGEKVILGIGLNVNNKVPDGATSMKQELGSEIPMLNVFK----- 158
N K+ G+L ++ V +G+GLNV+N+ P T + L P N+ K
Sbjct: 179 NGLKIGGILCTSTYRSRKFLVSVGVGLNVDNEQP--TTCLNAVLKDVCPPSNLLKREEIL 236
Query: 159 -TLVKTLDSLYLKFLESPGKILE 180
K ++ + F+E K LE
Sbjct: 237 GAFFKKFENFFDLFMEQGFKSLE 259
>sp|P06709|BIRA_ECOLI BIRA BIFUNCTIONAL PROTEIN [INCLUDES: BIOTIN OPERON REPRESSOR;
BIOTIN--[ACETYL-COA-CARBOXYLASE] SYNTHETASE
(BIOTIN--PROTEIN LIGASE)] >gi|73188|pir||BVECBF birA
bifunctional protein [validated] - Escherichia coli
>gi|442676|pdb|1BIA| Bira Bifunctional Protein (Acts As
Biotin Operon Repressor And Biotin Holoenzyme
Synthetase) (E.C.6.3.4.15) >gi|442677|pdb|1BIB| Bira
Bifunctional Protein (Acts As Biotin Operon Repressor
And Biotin Holoenzyme Synthetase) (E.C.6.3.4.15) Complex
With Biotinylated Lysine >gi|145432|gb|AAA23520.1|
(M10123) bifunctional protein [Escherichia coli]
>gi|145434|gb|AAA23521.1| (M15820) biotin-holoenzyme
synthetase [Escherichia coli] >gi|396316|gb|AAC43075.1|
(U00006) bifunctional protein: biotin operon repressor
and biotin-[acetyl-CoA carboxylase] synthetase
[Escherichia coli] >gi|1790408|gb|AAC76951.1| (AE000471)
biotin-[acetylCoA carboxylase] holoenzyme synthetase and
biotin operon repressor [Escherichia coli K12]
>gi|2852398|gb|AAC40211.1| (AF044308) Eschericia coli
biotin-holoenzyme synthetase [Cloning vector
pBIOTRX-BirA] >gi|4545270|gb|AAD22470.1|AF121784_2
(AF121784) biotin protein ligase [Expression vector
pCY216] >gi|4545273|gb|AAD22472.1| (AF121785) biotin
protein ligase [Expression vector pCY215]
Length = 321
Score = 81.1 bits (197), Expect = 1e-14
Identities = 69/242 (28%), Positives = 114/242 (46%), Gaps = 18/242 (7%)
Query: 10 GRTIIYFQEVASTNDYA--KAENLEEGTVIVADRQIKGHGRLERKWESPEGG-LWMSVVL 66
G ++ + STN Y + L+ G +A+ Q G GR RKW SP G L++S+
Sbjct: 78 GGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFW 137
Query: 67 TPRVSQEDLPKIVFLGALAVVETLREFSID-ARIKWPNDVLVNYRKVAGVLVEAKGE--- 122
+ + + + E LR+ D R+KWPND+ + RK+AG+LVE G+
Sbjct: 138 RLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGD 197
Query: 123 --KVILGIGLN-----VNNKVPDGATSMKQELGSEIPMLNVFKTLVKTLDSLYLKFLESP 175
++++G G+N V V + QE G + + L++ L + L+ E
Sbjct: 198 AAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAA-LELFEQE 256
Query: 176 G--KILERAKRSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVS 233
G L R ++ R L G+ E GI+ +D+ G L++ DG +K + G++S
Sbjct: 257 GLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLE-QDGIIKPWMGGEIS 315
Query: 234 LR 235
LR
Sbjct: 316 LR 317
>pir||D71657 biotin-protein ligase (birA) RP533 - Rickettsia prowazekii
>gi|3861082|emb|CAA14982.1| (AJ235272) BIOTIN-PROTEIN
LIGASE (birA) [Rickettsia prowazekii]
Length = 268
Score = 80.0 bits (194), Expect = 2e-14
Identities = 67/248 (27%), Positives = 117/248 (47%), Gaps = 47/248 (18%)
Query: 13 IIYFQEVASTND----YAKAENLEEGTVIVADRQIKGHGRLERKWESPEGGLWMSVVLTP 68
+I F E+ ST+ A+ + I A Q +G GR + W+S G L +S+++ P
Sbjct: 14 LIVFDEIDSTSSEAMRIARHNKVYADYAIFAKSQTRGRGRSGKNWQSRLGNLHVSLLMRP 73
Query: 69 RVSQEDLPKIVFLGALAVVETL-----------------------REFSIDARIKWPNDV 105
E LP++ F+ ALAV +T+ + +SI ++KWPND+
Sbjct: 74 DKELELLPQLCFVTALAVYDTILSCILYAGSNQLINNFNAVSVCPQNYSI--QLKWPNDI 131
Query: 106 LVNYRKVAGVLVEA----KGEKVILGIGLNVN---NKVPDGATSMKQELGSEIPMLNVFK 158
L+N RK+AG+L+E+ +I+GIG+N+ + TS+ E + + K
Sbjct: 132 LMNGRKIAGILLESVKMDNNYYLIIGIGINITYHPENIDQPTTSLITENLMHVVPKALLK 191
Query: 159 TLVKTLDSLYLKFLESPG------KILERAKRSMILGVRVKVLSDGEVEAEGIAEDVDEF 212
L+K + Y K G K +E A + + + +K +D G+ +D+D
Sbjct: 192 ILIKNFEK-YYKIWHHNGFPFIRKKWIEHAYK-LNENINIKYRND---IVTGLFKDIDNT 246
Query: 213 GRLIVRLD 220
G +I++L+
Sbjct: 247 GNIILQLN 254
>pir||S74680 biotin--[acetyl-CoA-carboxylase] ligase (EC 6.3.4.15) -
Synechocystis sp. (strain PCC 6803)
>gi|1651905|dbj|BAA16831.1| (D90901) biotin
[acetyl-CoA-carboxylase] ligase [Synechocystis sp.]
Length = 274
Score = 78.8 bits (191), Expect = 5e-14
Identities = 66/216 (30%), Positives = 102/216 (46%), Gaps = 24/216 (11%)
Query: 36 VIVADRQIKGHGRLERKWESPEGGLWMSVVLTPRVSQEDLPKIVFLGALAVVETLREFSI 95
V +A Q G G+ R W+SP GGL++S+ L + P +V A + L + I
Sbjct: 64 VAIAREQTAGRGQWGRTWQSPPGGLYLSLWLATDLPISYGPHLVLWSAWGIAHALIQHGI 123
Query: 96 DARIKWPNDVLVNYRKVAGVLVEAK--GEKV---ILGIGLNVNNKVPDGATSMKQELGSE 150
++KWPND+L+ +K+AG+ E+K G ++ I+G+G+N N VP ++ +E
Sbjct: 124 PVQVKWPNDLLLKGKKLAGIKTESKINGNRITAAIIGVGINWTNPVPATGIALGPFCEAE 183
Query: 151 -IPMLNVFKTLVK-TLDSLYLKFLESPGKILERAKRSMILGVRVKVL--------SDGEV 200
I +N L + TL L L + R +R I G+ V L
Sbjct: 184 SIQSINSLTDLAEITLAGLTLGW--------HRYQREGIAGILVDYLQLFAHRGREISLT 235
Query: 201 EAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVSLRF 236
+ GI E V G L+V + R + IL G VSL +
Sbjct: 236 QGVGIIESVTAQGELVVLIGQRR-EIILPGTVSLDY 270
>gb|AAF33493.1| (AF170176) Salmonella typhimurium bifunctional protein (BIRA)
(SW:P37416) [Salmonella typhimurium LT2]
Length = 320
Score = 77.3 bits (187), Expect = 2e-13
Identities = 70/232 (30%), Positives = 107/232 (45%), Gaps = 16/232 (6%)
Query: 19 VASTNDYA--KAENLEEGTVIVADRQIKGHGRLERKWESPEGG-LWMSVVLTPRVSQEDL 75
+ STN Y + L G VA+ Q G GR RKW SP G L++S+
Sbjct: 87 IDSTNQYLLDRIGELRSGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMYWRLEQGPAAA 146
Query: 76 PKIVFLGALAVVETLREFSID-ARIKWPNDVLVNYRKVAGVLVEAKGE-----KVILGIG 129
+ + + + E LR+ D R+KWPND+ + RK+AG+LVE G+ ++++G G
Sbjct: 147 IGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLLDRKLAGILVELTGKTGDAAQIVIGAG 206
Query: 130 LN-----VNNKVPDGATSMKQELGSEIPM-LNVFKTLVKTLDSLYLKFLESPGKILERAK 183
+N V V + QE G + + K + K +L L E L R K
Sbjct: 207 INMAMRRVEEDVINQGWITLQEAGITLDRNMLAAKLIYKLRAALELFEQEGLSPYLSRWK 266
Query: 184 RSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVSLR 235
+ R L G+ E GI+ +D G L++ DG +K + G++SLR
Sbjct: 267 KLDNFIDRPVKLIIGDKEIFGISRGIDTQGALLLE-QDGVIKPWMGGEISLR 317
>sp|P46363|BIRA_HAEIN BIRA BIFUNCTIONAL PROTEIN [INCLUDES: BIOTIN OPERON REPRESSOR;
BIOTIN--[ACETYL-COA-CARBOXYLASE] SYNTHETASE
(BIOTIN--PROTEIN LIGASE)] >gi|1573184|gb|AAC21889.1|
(U32708) biotin operon repressor/biotin acetyl coenzyme
A carboxylase synthetase (birA) [Haemophilus influenzae
Rd]
Length = 302
Score = 76.1 bits (184), Expect = 3e-13
Identities = 73/248 (29%), Positives = 120/248 (47%), Gaps = 26/248 (10%)
Query: 4 LKTSVIGRTIIYFQEVASTNDYAKAE--NLEEGTVIVADRQIKGHGRLERKWESPEGGLW 61
+ T++ +I Y ++STN++ +L++G + VA+ Q G GR R+W SP G
Sbjct: 63 ISTALFPYSIHYQPIISSTNEWILQNILSLKKGDLCVAEYQTAGRGRRGRQWLSPFAGQI 122
Query: 62 MSV---VLTPRVSQEDLPKIVFLGALAVVETLREFSIDARIKWPNDVLVNYRKVAGVLVE 118
M P+ S E L ++ LA+ E L + ++KWPND+L + RK+ G+LVE
Sbjct: 123 MFSFYWAFDPKKSIEGLSLVI---GLAIAEVL-----NVQVKWPNDILFDERKLGGILVE 174
Query: 119 AKGEK-----VILGIGLNVNNKVPDGATSMKQELGSEIPMLNVFKTLVKTLDSLY--LKF 171
K +++GIG+NV+ + E+ P + L K + LY L
Sbjct: 175 IANHKNGMLNLVIGIGINVSLSKQTEISQPYAEVCEIDPDVERQTLLPKLIQHLYTRLNI 234
Query: 172 LESPG--KILERAKRS--MILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKI 227
E G + ++A +S + VL++ V GI + +DE G L V L +++
Sbjct: 235 FEQNGIDEEFQQAWQSYNAFSNSEINVLTEQGV-ISGIEQGIDERGYLKV-LCGNKIQMF 292
Query: 228 LYGDVSLR 235
G+VSLR
Sbjct: 293 NGGEVSLR 300
>pir||C71334 probable biotin--acetyl-CoA-carboxylase ligase (birA) - syphilis
spirochete >gi|3322635|gb|AAC65343.1| (AE001215)
biotin--acetyl-CoA-carboxylase ligase (birA) [Treponema
pallidum]
Length = 246
Score = 74.1 bits (179), Expect = 1e-12
Identities = 63/174 (36%), Positives = 88/174 (50%), Gaps = 10/174 (5%)
Query: 10 GRTIIYFQEVASTNDYA--KAENLEEGTVIVADRQIKGHGRLE-RKWESPEGGLWMSVVL 66
G ++ + + AST D A +A+ EEG V A Q+ G GR + RKW+S G + V+
Sbjct: 15 GASVYFAPKTASTMDEAASRAQGCEEGAVFCAGEQVAGRGRGDQRKWQSEPGKNLLCTVV 74
Query: 67 TPRVSQEDLPKIV-FLGALAVVETLREFSIDARIKWPNDVLVNYRKVAGVLVEAKGEKVI 125
RV+ V + ALA + L RIKWPNDVLV RK+AGVL + + ++
Sbjct: 75 LRRVAFPAFSLCVGYAVALAYMAFLGGVCAP-RIKWPNDVLVCDRKIAGVLCQVRAGALL 133
Query: 126 LGIGLN-VNNKVP---DGATSMKQELGSEIPMLNVFKTLVKTLDSLYLKFLESP 175
+GIG N + K P A S+ Q +G E + F L LD LY + P
Sbjct: 134 VGIGCNLLQVKFPPELSHACSLAQIVGGE-RCPDPFAFLPVLLDRLYACVMAPP 186
>pir||B81009 BirA protein/Bvg accessory factor NMB2075 [imported] - Neisseria
meningitidis (group B strain MD58)
>gi|7227338|gb|AAF42394.1| (AE002557) BirA protein/Bvg
accessory factor [Neisseria meningitidis MC58]
Length = 592
Score = 73.4 bits (177), Expect = 2e-12
Identities = 67/236 (28%), Positives = 109/236 (45%), Gaps = 19/236 (8%)
Query: 18 EVASTNDYAK-----AENLEEGTVIVADRQIKGHGRLERKWESPEGG-LWMSVVLTPRVS 71
E AS+ND A + T+ V Q KG GR RKW G L S
Sbjct: 89 ECASSNDEILELARIAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRP 148
Query: 72 QEDLPKIVFLGALAVVETLREFSIDARIKWPNDVLVNYRKVAGVLVEA--KGEKVILGIG 129
Q +L + + A+A L +D +IKWPND++V K+ G+L+E G K + +G
Sbjct: 149 QYELGSLSPVAAVACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVG 208
Query: 130 LNVNNKVP---DGATSMKQELGSEIPMLN-----VFKTLVKTLDSLYLKFLES--PGKIL 179
+ +N +P + A S++ + N + +TL+ LD++ L++ +
Sbjct: 209 IGINFVLPKEVENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVA 268
Query: 180 ERAKRSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVSLR 235
E + G V +L DGE EG + VD G L + +G+ + ++ G++SLR
Sbjct: 269 EYQAANRDHGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGK-QTVVSGEISLR 323
>pir||B47751 biotin [acetyl-CoA-carboxylase] ligase homolog - Paracoccus
denitrificans
Length = 240
Score = 73.0 bits (176), Expect = 3e-12
Identities = 68/215 (31%), Positives = 102/215 (46%), Gaps = 21/215 (9%)
Query: 28 AENLEEGTVIVADRQIKGHGRLERKWESPEGGLWMSVVLTPRVSQEDLPKIVFLGALAVV 87
A L ++A Q G GR R+W P G ++VL P+ ++ F+ ALA+
Sbjct: 16 APGLSGSAWVLAREQFAGRGRRGREWVMPAGNFAGTLVLRPQGGALAAAQLSFVAALALY 75
Query: 88 ETLREFSIDAR---IKWPNDVLVNYRKVAGVLVEAKG-----EKVILGIGLNV-----NN 134
+ L A IKWPNDVL+N KVAG+L+E+ G + V +GIG+N+
Sbjct: 76 DALGLACGPAARLAIKWPNDVLLNGGKVAGILLESSGSGPGVQAVAVGIGVNLAGAPDAG 135
Query: 135 KVPDGAT---SMKQELGSEIPMLNVFKTLVKTLDSLYLKFLESPGKILER---AKRSMIL 188
V GAT S++ E G + F L+ + + L++ G R R+ L
Sbjct: 136 AVEPGATPPVSVQGETGHAVDP-EEFLDLLAPAFARWQAQLDTYGFAPIRNAWLARAARL 194
Query: 189 GVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGR 223
G + + G E+ GI E +D+ G LI+R GR
Sbjct: 195 GEPI-IARTGTAESHGIFEGIDDSGALILRGPAGR 228
>sp|P29906|BIRA_PARDE BIOTIN--[ACETYL-COA-CARBOXYLASE] SYNTHETASE (BIOTIN--PROTEIN
LIGASE) >gi|150617|gb|AAA25601.1| (L02354) biotin
[acetyl-CoA carboxylase] ligase [Paracoccus
denitrificans]
Length = 240
Score = 73.0 bits (176), Expect = 3e-12
Identities = 68/215 (31%), Positives = 102/215 (46%), Gaps = 21/215 (9%)
Query: 28 AENLEEGTVIVADRQIKGHGRLERKWESPEGGLWMSVVLTPRVSQEDLPKIVFLGALAVV 87
A L ++A Q G GR R+W P G ++VL P+ ++ F+ ALA+
Sbjct: 16 APGLSGSAWVLAREQFAGRGRRGREWVMPAGNFAGTLVLRPQGGALAAAQLSFVAALALY 75
Query: 88 ETLREFSIDAR---IKWPNDVLVNYRKVAGVLVEAKG-----EKVILGIGLNV-----NN 134
+ L A IKWPNDVL+N KVAG+L+E+ G + V +GIG+N+
Sbjct: 76 DALGLACGPAARLAIKWPNDVLLNGGKVAGILLESSGSGPGVQAVAVGIGVNLAGAPDAG 135
Query: 135 KVPDGAT---SMKQELGSEIPMLNVFKTLVKTLDSLYLKFLESPGKILER---AKRSMIL 188
V GAT S++ E G + F L+ + + L++ G R R+ L
Sbjct: 136 AVEPGATPPVSVQGETGHAVDP-EEFLDLLAPAFARWQAQLDTYGFAPIRNAWLARAARL 194
Query: 189 GVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGR 223
G + + G E+ GI E +D+ G LI+R GR
Sbjct: 195 GEPI-IARTGTAESHGIFEGIDDSGALILRGPAGR 228
>pir||H82031 probable BirA bifunctional protein NMA0357 [similarity] - Neisseria
meningitidis (group A strain Z2491)
>gi|7379110|emb|CAB83659.1| (AL162752) BirA bifunctional
protein [Neisseria meningitidis Z2491]
Length = 592
Score = 71.0 bits (171), Expect = 1e-11
Identities = 66/236 (27%), Positives = 108/236 (44%), Gaps = 19/236 (8%)
Query: 18 EVASTNDYAK-----AENLEEGTVIVADRQIKGHGRLERKWESPEGG-LWMSVVLTPRVS 71
E AS+ND A + T+ V Q KG GR RKW G L S
Sbjct: 89 ECASSNDEILELARIAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRP 148
Query: 72 QEDLPKIVFLGALAVVETLREFSIDARIKWPNDVLVNYRKVAGVLVEA--KGEKVILGIG 129
Q +L + + A+A L + +IKWPND++V K+ G+L+E G K + +G
Sbjct: 149 QYELGSLSPVAAVACRRALSRLGLKTQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVG 208
Query: 130 LNVNNKVP---DGATSMKQELGSEIPMLN-----VFKTLVKTLDSLYLKFLES--PGKIL 179
+ +N +P + A S++ + N + +TL+ LD++ L++ +
Sbjct: 209 IGINFVLPKEVENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPFVA 268
Query: 180 ERAKRSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVSLR 235
E + G V +L DGE EG + VD G L + +G+ + ++ G++SLR
Sbjct: 269 EYQAANRDHGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGK-QTVVSGEISLR 323
>pir||B72026 biotin synthetase - Chlamydophila pneumoniae (strains CWL029 and
AR39) >gi|4377183|gb|AAD19004.1| (AE001667) Biotin
Synthetase [Chlamydophila pneumoniae CWL029]
>gi|7189916|gb|AAF38781.1| (AE002257) BirA-related
protein [Chlamydophila pneumoniae AR39]
>gi|8979240|dbj|BAA99074.1| (AP002548) biotin synthetase
[Chlamydophila pneumoniae J138]
Length = 192
Score = 69.9 bits (168), Expect = 3e-11
Identities = 57/169 (33%), Positives = 85/169 (49%), Gaps = 17/169 (10%)
Query: 13 IIYFQ--EVASTNDYAKAE----NLEEGTVIVADRQIKGHGRLERKWESPEGGLWMSVVL 66
+IY++ E+ STN AK+ + TVI Q G G+ + W+S +G L +
Sbjct: 3 VIYYEIEEIPSTNTMAKSYMHLWDPYALTVISTKCQTAGTGKFGKSWKSSKGDLLNTFCF 62
Query: 67 TPRVSQEDLPKIVFLGALAVVETLREFSI-DARIKWPNDVLVNYRKVAGVLVEAKGEK-- 123
D+ ++ LG AVV ++ I +A+IKWPNDVLV+ K+ GVL E +
Sbjct: 63 FITDLHIDVSRLFRLGTEAVVALCKDLGITEAKIKWPNDVLVHGEKLCGVLPETLPVEGL 122
Query: 124 --VILGIGLNVN------NKVPDGATSMKQELGSEIPMLNVFKTLVKTL 164
V+LGIGLN N V ATS+++ LG I + + L+ L
Sbjct: 123 LGVVLGIGLNGNTTKQALKDVGQPATSLQEILGHPIDLETTRELLIHHL 171
>gb|AAC68833.1| (AF016461) putative biotin protein ligase [Bordetella pertussis]
Length = 288
Score = 69.5 bits (167), Expect = 3e-11
Identities = 72/256 (28%), Positives = 110/256 (42%), Gaps = 37/256 (14%)
Query: 11 RTIIYFQEVASTND----YAKAENLEEGTVIVADRQIKGHGRLERKWESPEGG-LWMSVV 65
R I + STN A+A ++ A Q G GR R W++ G L S
Sbjct: 36 RDISWVGSTGSTNADLLARARAGGGARPCLLGAHLQETGRGRAGRPWQNRVGAALMFSCA 95
Query: 66 LTPRVSQEDLPKIVFLGALAVVETLREF---SIDARIKWPNDVLVNYRKVAGVLVEA--- 119
+ LP + L +A E LR+ +KWPNDV + K+AGVLVE+
Sbjct: 96 FDVDLPPSQLPALSPLAGMAACEALRQLVGQPAGLLVKWPNDVQWHDAKLAGVLVESVRN 155
Query: 120 ---KGEKVILGIGLNVNNKVPDGATSMKQELGSEIPMLN-----------VFKTLVKTL- 164
G V++G+GLN++ G ++ Q L + N LV +
Sbjct: 156 PAGTGHAVVIGMGLNLH-----GGDALSQALQRSVADWNDVAGPGEKGPDAAADLVAAVA 210
Query: 165 ----DSLYLKFLESPGKILER-AKRSMILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRL 219
D+ + E G R A+ + G V V+ GE+ G A VD+ GRL+V+
Sbjct: 211 RAWQDAAHTLQQEGFGAFGARFARVDALAGRPVNVIDRGEILYTGAASGVDDHGRLLVQA 270
Query: 220 DDGRVKKILYGDVSLR 235
DG ++ + G++S+R
Sbjct: 271 ADG-MRPVSVGEISIR 285
>pir||B82637 bifunctional biotin operon/biotin acetyl-CoA-carboxylase synthetase
transcription repressor XF1796 [imported] - Xylella
fastidiosa (strain 9a5c)
>gi|9106873|gb|AAF84604.1|AE004001_9 (AE004001)
bifunctional transcriptional repressor of the biotin
operon/biotin acetyl-CoA-carboxylase synthetase [Xylella
fastidiosa]
Length = 317
Score = 68.7 bits (165), Expect = 6e-11
Identities = 64/229 (27%), Positives = 110/229 (47%), Gaps = 13/229 (5%)
Query: 18 EVASTNDYAKAENLEEGTVIVADRQIKGHGRLERKWESP-EGGLWMSVVLTPRVSQEDLP 76
E +T+ A+ + V++A+RQ G G+ R W SP +++S+ L
Sbjct: 89 ESTNTSLLARPALADGVEVLLAERQTGGRGQRGRIWISPLAANIYLSLSRVFSSGAAQLM 148
Query: 77 KIVFLGALAVVETLREFSID-ARIKWPNDVLVNYRKVAGVLVEAKGE-KVILGIGLNVN- 133
+ +A+ E L R+KWPND++ N RK+ GVLVE +GE + ++G+GLNV
Sbjct: 149 GLSVAVGVAIAEALIAAGFSHVRLKWPNDLVDNDRKLGGVLVERQGEARAVIGLGLNVRM 208
Query: 134 -----NKVPDGATSMKQELGSEIPMLNVFKTLVKT-LDSLYLKFLESPGKILER-AKRSM 186
++ ++ GS + + L+ L +L+L LE L R AK
Sbjct: 209 PTNFAEQIDQPWVDLETLAGSTVERNALVALLLSALLPALHLFQLEGLAPFLPRYAKLDC 268
Query: 187 ILGVRVKVLSDGEVEAEGIAEDVDEFGRLIVRLDDGRVKKILYGDVSLR 235
++G V + + ++ GIA + G L+V + +G ++ G VS+R
Sbjct: 269 LVGCAVWI-EEANLKYVGIARGLAADGALLVEI-NGVLRPFHTGQVSVR 315
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.318 0.139 0.387
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83160212
Number of Sequences: 2977
Number of extensions: 3352688
Number of successful extensions: 7526
Number of sequences better than 1.0e-10: 36
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 32
Number of HSP's that attempted gapping in prelim test: 7460
Number of HSP's gapped (non-prelim): 37
length of query: 237
length of database: 189,106,746
effective HSP length: 57
effective length of query: 180
effective length of database: 154,992,987
effective search space: 27898737660
effective search space used: 27898737660
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 164 (68.3 bits)