BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0104 (birA-like) DE:related biotin operon
repressor/biotin--[acetyl CoA carboxylase] ligase
(227 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||A75204 related biotin operon repressor/biotin-- [acetyl coa... 458 e-128
gi|11497694 biotin operon repressor/biotin--[acetyl CoA carboxyl... 98 1e-19
dbj|BAB05404.1| (AP001512) transcriptional repressor of the biot... 84 2e-15
sp|P42975|BIRA_BACSU BIRA BIFUNCTIONAL PROTEIN [INCLUDES: BIOTIN... 78 9e-14
gb|AAB60184.1| (U20445) BirA protein [Bacillus subtilis] 75 8e-13
>pir||A75204 related biotin operon repressor/biotin-- [acetyl coa carboxylase]
ligase PAB0104 - Pyrococcus abyssi (strain Orsay)
>gi|5457589|emb|CAB49080.1| (AJ248283) related biotin
operon repressor/biotin--[acetyl CoA carboxylase] ligase
[Pyrococcus abyssi]
Length = 227
Score = 458 bits (1167), Expect = e-128
Identities = 227/227 (100%), Positives = 227/227 (100%)
Query: 1 MRCIAPGPKFRILEMLKAGKRVSGELIARELGISRIAVWKHVKMLKSLGYEIDARRNGYR 60
MRCIAPGPKFRILEMLKAGKRVSGELIARELGISRIAVWKHVKMLKSLGYEIDARRNGYR
Sbjct: 1 MRCIAPGPKFRILEMLKAGKRVSGELIARELGISRIAVWKHVKMLKSLGYEIDARRNGYR 60
Query: 61 LVKIPDEPFPWELGVNYLYCLKVRSTMDSAWRNFGTYDGVIAKEQTKGRGRSDPWISLPG 120
LVKIPDEPFPWELGVNYLYCLKVRSTMDSAWRNFGTYDGVIAKEQTKGRGRSDPWISLPG
Sbjct: 61 LVKIPDEPFPWELGVNYLYCLKVRSTMDSAWRNFGTYDGVIAKEQTKGRGRSDPWISLPG 120
Query: 121 GLYLSACFPRISSVSMVETLANDIAESLSERYSLNVQAVEGKLFLNGKKLGGVLIEVRGN 180
GLYLSACFPRISSVSMVETLANDIAESLSERYSLNVQAVEGKLFLNGKKLGGVLIEVRGN
Sbjct: 121 GLYLSACFPRISSVSMVETLANDIAESLSERYSLNVQAVEGKLFLNGKKLGGVLIEVRGN 180
Query: 181 YKAVVGVGINVRNPVPRNFARLENASLREVAEIVIKVLQRYNRRERT 227
YKAVVGVGINVRNPVPRNFARLENASLREVAEIVIKVLQRYNRRERT
Sbjct: 181 YKAVVGVGINVRNPVPRNFARLENASLREVAEIVIKVLQRYNRRERT 227
>gi|11497694 biotin operon repressor/biotin--[acetyl CoA carboxylase] ligase
(birA) [Archaeoglobus fulgidus] >gi|7450984|pir||B69259
biotin operon repressor/biotin--[acetyl CoA carboxylase]
ligase (birA) homolog - Archaeoglobus fulgidus
>gi|2650573|gb|AAB91153.1| (AE001101) biotin operon
repressor/biotin--[acetyl CoA carboxylase] ligase (birA)
[Archaeoglobus fulgidus]
Length = 299
Score = 97.5 bits (239), Expect = 1e-19
Identities = 74/231 (32%), Positives = 114/231 (49%), Gaps = 17/231 (7%)
Query: 1 MRCIAPGPKFRILEMLKAGKRVSGELIARELGISRIAVWKHVKMLKSLGYEIDARRNGYR 60
MR +R+ +L +SGE +A++LGISR AVWK V+ LK G +++ +GY
Sbjct: 1 MRISRESTDYRVYRILSENP-LSGEKLAKKLGISRTAVWKAVQKLKECGVYVESNASGYT 59
Query: 61 LVKIPDEPFPWEL-------GVNYLYCLKVRSTMDSAWRNFGTYDGVI-AKEQTKGRGRS 112
+ +E P+E+ G ++ V + +S + +G D + A QT GRGR
Sbjct: 60 IAG-EEELNPYEVARLAFESGFKEVHFYDVTDSTNSRAKEYGKPDALFFANRQTAGRGRH 118
Query: 113 D-PWISLPGGLYLSACFPRISSVSMVETLANDIAESLSERYSLNVQAVEGKLFLNGKKLG 171
W+S GGLY S S + L S++E + + + G+K+
Sbjct: 119 GRKWLSEEGGLYFSVTLSPPLDYSELPKLTLIAGLSVAEAIPQSEIKWPNDVLIKGRKVC 178
Query: 172 GVLIEVRGNYK---AVVGVGINVRNPVPRN---FARLENASLREVAEIVIK 216
G+L E+ G + +VGVGINV+NP P N + L + S REV E VI+
Sbjct: 179 GILSELHGEVERPLVIVGVGINVKNPHPENGISLSELYDVSRREVFEQVIR 229
>dbj|BAB05404.1| (AP001512) transcriptional repressor of the biotin operon [Bacillus
halodurans]
Length = 323
Score = 83.9 bits (204), Expect = 2e-15
Identities = 64/197 (32%), Positives = 99/197 (49%), Gaps = 25/197 (12%)
Query: 22 VSGELIARELGISRIAVWKHVKMLKSLGYEIDA-RRNGYRLVKIPDEPFPWELGV----- 75
VSGE I++ +G SR AVWKH++ L+ GYE++A +R GYR+VK PD+ P ++ V
Sbjct: 17 VSGEKISQAIGCSRTAVWKHIEELRKSGYEVEAVQRKGYRIVKRPDQIKPHDIQVVLETE 76
Query: 76 ------NYLYCLKVRSTMDSAWRNFGTYDG--VIAKEQTKGRGR-SDPWISLPG-GLYLS 125
YL T+ G +G V+A EQT G+GR W S PG + +S
Sbjct: 77 RFGREITYLESTASTQTVALKLAQEGAKEGHIVLANEQTSGKGRMGRGWYSPPGSSISMS 136
Query: 126 ACF----PRISSVSMVETLANDIAESLSERYSLNVQAV-EGKLFLNGKKLGGVLIEVRGN 180
F P + + A I ++ E L+ L ++GKK+ G+L E++ +
Sbjct: 137 IIFRPQLPPQKAPQLTLLTAVAIVRAIKETTGLDSDIKWPNDLLIDGKKIVGILTEMQAD 196
Query: 181 ----YKAVVGVGINVRN 193
+ + G+GINV +
Sbjct: 197 QDSVHSVIQGIGINVNH 213
>sp|P42975|BIRA_BACSU BIRA BIFUNCTIONAL PROTEIN [INCLUDES: BIOTIN OPERON REPRESSOR;
BIOTIN--[ACETYL-COA-CARBOXYLASE] SYNTHETASE
(BIOTIN--PROTEIN LIGASE)] >gi|7450978|pir||A69595
transcription repressor of biotin operon birA - Bacillus
subtilis >gi|1146239|gb|AAB38447.1| (L47709) biotin
[acetyl-CoA-carboxylase] ligase [Bacillus subtilis]
>gi|2634662|emb|CAB14160.1| (Z99115) transcriptional
regulator and biotin acetyl-CoA-carboxylase synthetase
[Bacillus subtilis]
Length = 325
Score = 78.0 bits (189), Expect = 9e-14
Identities = 65/203 (32%), Positives = 98/203 (48%), Gaps = 26/203 (12%)
Query: 22 VSGELIARELGISRIAVWKHVKMLKSLGYEIDA-RRNGYRLVKIPDEPFPWE-------- 72
+SG+ I+ LG SR AVWKH++ L+ GYE++A RR GYRL+K P + E
Sbjct: 21 ISGQKISDALGCSRTAVWKHIEELRKEGYEVEAVRRKGYRLIKKPGKLSESEIRFGLKTE 80
Query: 73 -LGVNYLYCLKVRSTMDSAWR--NFGTYDG--VIAKEQTKGRGR-SDPWISLPG-----G 121
+G + +Y + ST +A N +G V+A +QT GRGR S W S G
Sbjct: 81 VMGQHLIYHDVLSSTQKTAHELANNNAPEGTLVVADKQTAGRGRMSRVWHSQEGNGVWMS 140
Query: 122 LYLSACFPRISSVSMVETLANDIAESLSERYSLNVQAV-EGKLFLNGKKLGGVLIEVRGN 180
L L P + + A + + + E + + +NGKK G+L E++
Sbjct: 141 LILRPDIPLQKTPQLTLLAAVAVVQGIEEAAGIQTDIKWPNDILINGKKTVGILTEMQAE 200
Query: 181 ----YKAVVGVGINVRNPVPRNF 199
++G+GINV N P +F
Sbjct: 201 EDRVRSVIIGIGINV-NQQPNDF 222
>gb|AAB60184.1| (U20445) BirA protein [Bacillus subtilis]
Length = 325
Score = 74.9 bits (181), Expect = 8e-13
Identities = 61/195 (31%), Positives = 96/195 (48%), Gaps = 25/195 (12%)
Query: 22 VSGELIARELGISRIAVWKHVKMLKSLGYEIDA-RRNGYRLVKIPDEPFPWE-------- 72
+SG+ I+ LG SR AVWKH++ L+ GYE++A RR GYRL+K P + E
Sbjct: 21 ISGQKISDALGCSRTAVWKHIEELRKEGYEVEAVRRKGYRLIKKPGKLSESEIRFGLKTE 80
Query: 73 -LGVNYLYCLKVRSTMDSAWR--NFGTYDG--VIAKEQTKGRGR-SDPWISLPG-GLYLS 125
+G + +Y + ST +A N +G V+A +QT GRGR S W S G G+++S
Sbjct: 81 VMGQHLIYQDVISSTQKTAHELANNNAPEGTLVVADKQTAGRGRMSRVWHSQEGNGIWMS 140
Query: 126 AC----FPRISSVSMVETLANDIAESLSERYSLNVQAV-EGKLFLNGKKLGGVLIEVRGN 180
P + + A + + + + + +NGKK G+L E++
Sbjct: 141 LILRPDIPLQKTPQLTLLAAVAVVQGIEAAAGIQTDIKWPNDILINGKKTVGILTEMQAE 200
Query: 181 ----YKAVVGVGINV 191
++G+GINV
Sbjct: 201 EDRVRSVIIGIGINV 215
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.321 0.139 0.412
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 82835377
Number of Sequences: 2977
Number of extensions: 3285067
Number of successful extensions: 10853
Number of sequences better than 1.0e-10: 5
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 10842
Number of HSP's gapped (non-prelim): 6
length of query: 227
length of database: 189,106,746
effective HSP length: 53
effective length of query: 174
effective length of database: 157,386,935
effective search space: 27385326690
effective search space used: 27385326690
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 163 (67.9 bits)