BLASTP 2.0.10 [Aug-26-1999]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= PAB0104 (birA-like) DE:related biotin operon
repressor/biotin--[acetyl CoA carboxylase] ligase
         (227 letters)

Database: ./suso.pep; /banques/blast2/nr.pep
           598,487 sequences; 189,106,746 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||A75204 related biotin operon repressor/biotin-- [acetyl coa...   458  e-128
gi|11497694 biotin operon repressor/biotin--[acetyl CoA carboxyl...    98  1e-19
dbj|BAB05404.1| (AP001512) transcriptional repressor of the biot...    84  2e-15
sp|P42975|BIRA_BACSU BIRA BIFUNCTIONAL PROTEIN [INCLUDES: BIOTIN...    78  9e-14
gb|AAB60184.1| (U20445) BirA protein [Bacillus subtilis]               75  8e-13

>pir||A75204 related biotin operon repressor/biotin-- [acetyl coa carboxylase]
           ligase PAB0104 - Pyrococcus abyssi (strain Orsay)
           >gi|5457589|emb|CAB49080.1| (AJ248283) related biotin
           operon repressor/biotin--[acetyl CoA carboxylase] ligase
           [Pyrococcus abyssi]
           Length = 227
           
 Score =  458 bits (1167), Expect = e-128
 Identities = 227/227 (100%), Positives = 227/227 (100%)

Query: 1   MRCIAPGPKFRILEMLKAGKRVSGELIARELGISRIAVWKHVKMLKSLGYEIDARRNGYR 60
           MRCIAPGPKFRILEMLKAGKRVSGELIARELGISRIAVWKHVKMLKSLGYEIDARRNGYR
Sbjct: 1   MRCIAPGPKFRILEMLKAGKRVSGELIARELGISRIAVWKHVKMLKSLGYEIDARRNGYR 60

Query: 61  LVKIPDEPFPWELGVNYLYCLKVRSTMDSAWRNFGTYDGVIAKEQTKGRGRSDPWISLPG 120
           LVKIPDEPFPWELGVNYLYCLKVRSTMDSAWRNFGTYDGVIAKEQTKGRGRSDPWISLPG
Sbjct: 61  LVKIPDEPFPWELGVNYLYCLKVRSTMDSAWRNFGTYDGVIAKEQTKGRGRSDPWISLPG 120

Query: 121 GLYLSACFPRISSVSMVETLANDIAESLSERYSLNVQAVEGKLFLNGKKLGGVLIEVRGN 180
           GLYLSACFPRISSVSMVETLANDIAESLSERYSLNVQAVEGKLFLNGKKLGGVLIEVRGN
Sbjct: 121 GLYLSACFPRISSVSMVETLANDIAESLSERYSLNVQAVEGKLFLNGKKLGGVLIEVRGN 180

Query: 181 YKAVVGVGINVRNPVPRNFARLENASLREVAEIVIKVLQRYNRRERT 227
           YKAVVGVGINVRNPVPRNFARLENASLREVAEIVIKVLQRYNRRERT
Sbjct: 181 YKAVVGVGINVRNPVPRNFARLENASLREVAEIVIKVLQRYNRRERT 227


>gi|11497694 biotin operon repressor/biotin--[acetyl CoA carboxylase] ligase
           (birA) [Archaeoglobus fulgidus] >gi|7450984|pir||B69259
           biotin operon repressor/biotin--[acetyl CoA carboxylase]
           ligase (birA) homolog - Archaeoglobus fulgidus
           >gi|2650573|gb|AAB91153.1| (AE001101) biotin operon
           repressor/biotin--[acetyl CoA carboxylase] ligase (birA)
           [Archaeoglobus fulgidus]
           Length = 299
           
 Score = 97.5 bits (239), Expect = 1e-19
 Identities = 74/231 (32%), Positives = 114/231 (49%), Gaps = 17/231 (7%)

Query: 1   MRCIAPGPKFRILEMLKAGKRVSGELIARELGISRIAVWKHVKMLKSLGYEIDARRNGYR 60
           MR       +R+  +L     +SGE +A++LGISR AVWK V+ LK  G  +++  +GY 
Sbjct: 1   MRISRESTDYRVYRILSENP-LSGEKLAKKLGISRTAVWKAVQKLKECGVYVESNASGYT 59

Query: 61  LVKIPDEPFPWEL-------GVNYLYCLKVRSTMDSAWRNFGTYDGVI-AKEQTKGRGRS 112
           +    +E  P+E+       G   ++   V  + +S  + +G  D +  A  QT GRGR 
Sbjct: 60  IAG-EEELNPYEVARLAFESGFKEVHFYDVTDSTNSRAKEYGKPDALFFANRQTAGRGRH 118

Query: 113 D-PWISLPGGLYLSACFPRISSVSMVETLANDIAESLSERYSLNVQAVEGKLFLNGKKLG 171
              W+S  GGLY S         S +  L      S++E    +       + + G+K+ 
Sbjct: 119 GRKWLSEEGGLYFSVTLSPPLDYSELPKLTLIAGLSVAEAIPQSEIKWPNDVLIKGRKVC 178

Query: 172 GVLIEVRGNYK---AVVGVGINVRNPVPRN---FARLENASLREVAEIVIK 216
           G+L E+ G  +    +VGVGINV+NP P N    + L + S REV E VI+
Sbjct: 179 GILSELHGEVERPLVIVGVGINVKNPHPENGISLSELYDVSRREVFEQVIR 229


>dbj|BAB05404.1| (AP001512) transcriptional repressor of the biotin operon [Bacillus
           halodurans]
           Length = 323
           
 Score = 83.9 bits (204), Expect = 2e-15
 Identities = 64/197 (32%), Positives = 99/197 (49%), Gaps = 25/197 (12%)

Query: 22  VSGELIARELGISRIAVWKHVKMLKSLGYEIDA-RRNGYRLVKIPDEPFPWELGV----- 75
           VSGE I++ +G SR AVWKH++ L+  GYE++A +R GYR+VK PD+  P ++ V     
Sbjct: 17  VSGEKISQAIGCSRTAVWKHIEELRKSGYEVEAVQRKGYRIVKRPDQIKPHDIQVVLETE 76

Query: 76  ------NYLYCLKVRSTMDSAWRNFGTYDG--VIAKEQTKGRGR-SDPWISLPG-GLYLS 125
                  YL       T+       G  +G  V+A EQT G+GR    W S PG  + +S
Sbjct: 77  RFGREITYLESTASTQTVALKLAQEGAKEGHIVLANEQTSGKGRMGRGWYSPPGSSISMS 136

Query: 126 ACF----PRISSVSMVETLANDIAESLSERYSLNVQAV-EGKLFLNGKKLGGVLIEVRGN 180
             F    P   +  +    A  I  ++ E   L+        L ++GKK+ G+L E++ +
Sbjct: 137 IIFRPQLPPQKAPQLTLLTAVAIVRAIKETTGLDSDIKWPNDLLIDGKKIVGILTEMQAD 196

Query: 181 ----YKAVVGVGINVRN 193
               +  + G+GINV +
Sbjct: 197 QDSVHSVIQGIGINVNH 213


>sp|P42975|BIRA_BACSU BIRA BIFUNCTIONAL PROTEIN [INCLUDES: BIOTIN OPERON REPRESSOR;
           BIOTIN--[ACETYL-COA-CARBOXYLASE] SYNTHETASE
           (BIOTIN--PROTEIN LIGASE)] >gi|7450978|pir||A69595
           transcription repressor of biotin operon birA - Bacillus
           subtilis >gi|1146239|gb|AAB38447.1| (L47709) biotin
           [acetyl-CoA-carboxylase] ligase [Bacillus subtilis]
           >gi|2634662|emb|CAB14160.1| (Z99115) transcriptional
           regulator and biotin acetyl-CoA-carboxylase synthetase
           [Bacillus subtilis]
           Length = 325
           
 Score = 78.0 bits (189), Expect = 9e-14
 Identities = 65/203 (32%), Positives = 98/203 (48%), Gaps = 26/203 (12%)

Query: 22  VSGELIARELGISRIAVWKHVKMLKSLGYEIDA-RRNGYRLVKIPDEPFPWE-------- 72
           +SG+ I+  LG SR AVWKH++ L+  GYE++A RR GYRL+K P +    E        
Sbjct: 21  ISGQKISDALGCSRTAVWKHIEELRKEGYEVEAVRRKGYRLIKKPGKLSESEIRFGLKTE 80

Query: 73  -LGVNYLYCLKVRSTMDSAWR--NFGTYDG--VIAKEQTKGRGR-SDPWISLPG-----G 121
            +G + +Y   + ST  +A    N    +G  V+A +QT GRGR S  W S  G      
Sbjct: 81  VMGQHLIYHDVLSSTQKTAHELANNNAPEGTLVVADKQTAGRGRMSRVWHSQEGNGVWMS 140

Query: 122 LYLSACFPRISSVSMVETLANDIAESLSERYSLNVQAV-EGKLFLNGKKLGGVLIEVRGN 180
           L L    P   +  +    A  + + + E   +         + +NGKK  G+L E++  
Sbjct: 141 LILRPDIPLQKTPQLTLLAAVAVVQGIEEAAGIQTDIKWPNDILINGKKTVGILTEMQAE 200

Query: 181 ----YKAVVGVGINVRNPVPRNF 199
                  ++G+GINV N  P +F
Sbjct: 201 EDRVRSVIIGIGINV-NQQPNDF 222


>gb|AAB60184.1| (U20445) BirA protein [Bacillus subtilis]
           Length = 325
           
 Score = 74.9 bits (181), Expect = 8e-13
 Identities = 61/195 (31%), Positives = 96/195 (48%), Gaps = 25/195 (12%)

Query: 22  VSGELIARELGISRIAVWKHVKMLKSLGYEIDA-RRNGYRLVKIPDEPFPWE-------- 72
           +SG+ I+  LG SR AVWKH++ L+  GYE++A RR GYRL+K P +    E        
Sbjct: 21  ISGQKISDALGCSRTAVWKHIEELRKEGYEVEAVRRKGYRLIKKPGKLSESEIRFGLKTE 80

Query: 73  -LGVNYLYCLKVRSTMDSAWR--NFGTYDG--VIAKEQTKGRGR-SDPWISLPG-GLYLS 125
            +G + +Y   + ST  +A    N    +G  V+A +QT GRGR S  W S  G G+++S
Sbjct: 81  VMGQHLIYQDVISSTQKTAHELANNNAPEGTLVVADKQTAGRGRMSRVWHSQEGNGIWMS 140

Query: 126 AC----FPRISSVSMVETLANDIAESLSERYSLNVQAV-EGKLFLNGKKLGGVLIEVRGN 180
                  P   +  +    A  + + +     +         + +NGKK  G+L E++  
Sbjct: 141 LILRPDIPLQKTPQLTLLAAVAVVQGIEAAAGIQTDIKWPNDILINGKKTVGILTEMQAE 200

Query: 181 ----YKAVVGVGINV 191
                  ++G+GINV
Sbjct: 201 EDRVRSVIIGIGINV 215


  Database: ./suso.pep
    Posted date:  Jul 6, 2001  5:57 PM
  Number of letters in database: 840,471
  Number of sequences in database:  2977
  
  Database: /banques/blast2/nr.pep
    Posted date:  Dec 14, 2000 12:46 PM
  Number of letters in database: 188,266,275
  Number of sequences in database:  595,510
  
Lambda     K      H
   0.321    0.139    0.412 

Gapped
Lambda     K      H
   0.270   0.0470    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 82835377
Number of Sequences: 2977
Number of extensions: 3285067
Number of successful extensions: 10853
Number of sequences better than 1.0e-10: 5
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 10842
Number of HSP's gapped (non-prelim): 6
length of query: 227
length of database: 189,106,746
effective HSP length: 53
effective length of query: 174
effective length of database: 157,386,935
effective search space: 27385326690
effective search space used: 27385326690
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 163 (67.9 bits)