BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0110 (moaE) DE:molybdopterin converting factor, subunit 2
(moaE)
(148 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||D75205 molybdopterin biosynthesis protein E chain PAB0110 [... 299 9e-81
pir||F71240 molybdopterin biosynthesis protein E chain [similari... 245 1e-64
pir||D70487 molybdopterin biosynthesis protein E chain [similari... 110 7e-24
sp|O05795|MOAE_MYCTU MOLYBDOPTERIN [MPT] CONVERTING FACTOR, SUBU... 109 2e-23
emb|CAC12598.1| (AL445067) molybdopterin-synthase large subunit ... 105 2e-22
sp|P30749|MOAE_ECOLI MOLYBDOPTERIN [MPT] CONVERTING FACTOR, SUBU... 105 3e-22
pir||B82251 molybdenum cofactor biosynthesis protein E VC1028 [i... 104 4e-22
pir||B69659 molybdopterin biosynthesis protein E chain [similari... 102 2e-21
gb|AAD39471.1|AF138285_1 (AF138285) molybdopterin synthase large... 100 1e-20
gb|AAB64030.1| (AC002333) putative molybdopterin synthase large ... 97 9e-20
pir||B83156 molybdopterin converting factor, large subunit PA391... 97 9e-20
gi|4758732 molybdenum cofactor synthesis 2 [Homo sapiens] >gi|11... 97 1e-19
sp|P45308|MOAE_HAEIN MOLYBDOPTERIN [MPT] CONVERTING FACTOR, SUBU... 96 2e-19
dbj|BAB06738.1| (AP001517) molybdopterin converting factor (subu... 96 2e-19
pir||B70844 molybdopterin biosynthesis protein D/E Rv3323c [simi... 96 3e-19
emb|CAC01331.1| (AL390968) moaE-like protein [Streptomyces coeli... 94 6e-19
gb|AAC83141.1| (AF109295) MoaE [Staphylococcus carnosus] 93 1e-18
sp|Q55371|Y903_SYNY3 HYPOTHETICAL 16.5 KD PROTEIN SLR0903 >gi|74... 92 4e-18
gi|11499761 molybdopterin converting factor, subunit 2 (moaE) [A... 88 4e-17
gi|7305279 molybdenum cofactor synthesis 2 [Mus musculus] >gi|42... 87 7e-17
gb|AAC44004.1| (U29587) homologous to moaE gene of E. coli; Meth... 86 2e-16
pir||E75252 molybdopterin biosynthesis protein D/E DR2607 [simil... 85 4e-16
pir||B70816 probable molybdopterin biosynthesis protein E2 chain... 83 2e-15
pir||T44853 molybdopterin biosynthesis protein E chain [similari... 82 3e-15
sp|Q56210|MOAE_SYNP7 MOLYBDOPTERIN [MPT] CONVERTING FACTOR, SUBU... 79 2e-14
pir||T25919 hypothetical protein T27A3.6 - Caenorhabditis elegan... 79 3e-14
pir||E72646 probable molybdopterin (mpt) converting factor, subu... 76 1e-13
gb|AAG18716.1| (AE004976) molybdenum cofactor biosynthesis prote... 75 5e-13
gb|AAK42541.1| Molybdenum cofactor biosynthesis protein E (moaE)... 70 1e-11
gb|AAF76450.1|AF077841_1 (AF077841) putative MoaD2 [Sulfolobus s... 70 1e-11
>pir||D75205 molybdopterin biosynthesis protein E chain PAB0110 [similarity] -
Pyrococcus abyssi (strain Orsay)
>gi|5457600|emb|CAB49091.1| (AJ248283) molybdopterin
converting factor, subunit 2 (moaE) [Pyrococcus abyssi]
Length = 148
Score = 299 bits (758), Expect = 9e-81
Identities = 148/148 (100%), Positives = 148/148 (100%)
Query: 1 MGKSKVSLVKKPEDFNIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMA 60
MGKSKVSLVKKPEDFNIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMA
Sbjct: 1 MGKSKVSLVKKPEDFNIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMA 60
Query: 61 IKEMERIRNEALDRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVK 120
IKEMERIRNEALDRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVK
Sbjct: 61 IKEMERIRNEALDRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVK 120
Query: 121 KRVPVWKREVTDEGEFWIEGDRHVPVKR 148
KRVPVWKREVTDEGEFWIEGDRHVPVKR
Sbjct: 121 KRVPVWKREVTDEGEFWIEGDRHVPVKR 148
>pir||F71240 molybdopterin biosynthesis protein E chain [similarity] -
Pyrococcus horikoshii >gi|3256570|dbj|BAA29253.1|
(AP000001) 149aa long hypothetical
molybdopterinconverting factor, subunit [Pyrococcus
horikoshii]
Length = 149
Score = 245 bits (620), Expect = 1e-64
Identities = 110/145 (75%), Positives = 134/145 (91%)
Query: 1 MGKSKVSLVKKPEDFNIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMA 60
M SK++L+KKP DF+I AI+L+SSSSTGGIVIFLGKVRDEN GR+V++LIYEAY+EMA
Sbjct: 1 MEDSKIALIKKPADFDIARAISLISSSSTGGIVIFLGKVRDENFGRRVKRLIYEAYEEMA 60
Query: 61 IKEMERIRNEALDRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVK 120
+KEME+IR EAL +FPI+DALIWHR+GELE+GENTILV+ S+KHRREAFEAC W+VDEVK
Sbjct: 61 LKEMEKIRKEALSKFPIIDALIWHRVGELEIGENTILVIASSKHRREAFEACIWMVDEVK 120
Query: 121 KRVPVWKREVTDEGEFWIEGDRHVP 145
KRVP+WKRE+T+EGE+WIEGD+ VP
Sbjct: 121 KRVPIWKREITEEGEYWIEGDKAVP 145
>pir||D70487 molybdopterin biosynthesis protein E chain [similarity] - Aquifex
aeolicus >gi|2984359|gb|AAC07878.1| (AE000776)
molybdopterin converting factor subunit 2 [Aquifex
aeolicus]
Length = 154
Score = 110 bits (273), Expect = 7e-24
Identities = 54/115 (46%), Positives = 78/115 (66%), Gaps = 2/115 (1%)
Query: 30 GGIVIFLGKVRDE-NLGRKVEKLIYEAYDEMAIKEMERIRNEALDRFPIVDALIWHRIGE 88
G V+FLG R+ G +E L YEAY EMAIKEME+IR E +++F + + I HR+G
Sbjct: 32 GAQVLFLGIPRNAPEDGGNIEALEYEAYPEMAIKEMEKIRQETIEKFGVKEVFIHHRLGL 91
Query: 89 LEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKREVTDEGE-FWIEGDR 142
+++GE + LV+ HR E F+AC + VDE KKRVP+WK+E+ EG+ W+ G++
Sbjct: 92 VKIGEPSFLVLAVGGHREETFKACRYAVDETKKRVPIWKKEIFKEGKGEWVLGEK 146
>sp|O05795|MOAE_MYCTU MOLYBDOPTERIN [MPT] CONVERTING FACTOR, SUBUNIT 2 (MOLYBDENUM
COFACTOR BIOSYNTHESIS PROTEIN E) (MOLYBDOPTERIN
CONVERTING FACTOR LARGE SUBUNIT) >gi|7449545|pir||F70921
probable molybdopterin biosynthesis protein E1 chain
[similarity] - Mycobacterium tuberculosis (strain H37RV)
>gi|2076694|emb|CAB08376.1| (Z95150) moaE [Mycobacterium
tuberculosis]
Length = 147
Score = 109 bits (269), Expect = 2e-23
Identities = 52/130 (40%), Positives = 78/130 (60%), Gaps = 1/130 (0%)
Query: 13 EDFNIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEMERIRNEAL 72
+ +++E +A VS GGIVIF+G VRD N G V +L YEAY M I+ + I
Sbjct: 18 QPLSVDEVLAAVSGPEQGGIVIFVGNVRDHNAGHDVTRLFYEAYPPMVIRTLMSIIGRCE 77
Query: 73 DRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKREVTD 132
D+ V + HR GEL++G+ +++ SA HR EAF+A ++ +K+ VP+WK+E +
Sbjct: 78 DKAEGVRVAVAHRTGELQIGDAAVVIGASAPHRAEAFDAARMCIELLKQEVPIWKKEFSS 137
Query: 133 EGEFWIEGDR 142
G W+ GDR
Sbjct: 138 TGAEWV-GDR 146
>emb|CAC12598.1| (AL445067) molybdopterin-synthase large subunit related protein
[Thermoplasma acidophilum]
Length = 135
Score = 105 bits (260), Expect = 2e-22
Identities = 46/114 (40%), Positives = 78/114 (68%)
Query: 17 IEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEMERIRNEALDRFP 76
+ + I + + G IV F G VR+++ G KV+ L YE+Y EMAI+E+ + + +F
Sbjct: 13 VMDLIDRIRNPKAGAIVTFEGTVREDSDGVKVDALFYESYREMAIREIRDMIEYCIRQFG 72
Query: 77 IVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKREV 130
I DA + HRIG + + E+++++ VSA HR +AF+AC +I+D++K+R P+WKR++
Sbjct: 73 ITDAAVVHRIGRVNLTEDSVVIAVSAPHRGQAFDACRYIIDQIKERAPIWKRDI 126
>sp|P30749|MOAE_ECOLI MOLYBDOPTERIN [MPT] CONVERTING FACTOR, SUBUNIT 2 (MOLYBDENUM
COFACTOR BIOSYNTHESIS PROTEIN E) (MOLYBDOPTERIN
CONVERTING FACTOR LARGE SUBUNIT) >gi|321846|pir||S31883
molybdopterin biosynthesis protein E chain [validated] -
Escherichia coli >gi|42012|emb|CAA49865.1| (X70420) moaE
[Escherichia coli] >gi|1787003|gb|AAC73872.1| (AE000181)
molybdopterin converting factor, subunit 2 [Escherichia
coli K12] >gi|4062341|dbj|BAA35443.1| (D90716)
Molybdopterin-converting factor 16k chain [Escherichia
coli]
Length = 150
Score = 105 bits (259), Expect = 3e-22
Identities = 56/137 (40%), Positives = 79/137 (56%), Gaps = 1/137 (0%)
Query: 4 SKVSLVKKPEDFNI-EEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIK 62
++ +V P+ F++ EE L G +V F GKVR+ NLG V L E Y M K
Sbjct: 2 AETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLGDSVNALTLEHYPGMTEK 61
Query: 63 EMERIRNEALDRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKR 122
+ I +EA +R+P+ + HRIGEL G+ + V V++ HR AFEA +I+D +K R
Sbjct: 62 ALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSAHRSSAFEAGQFIMDYLKTR 121
Query: 123 VPVWKREVTDEGEFWIE 139
P WKRE T EG+ W+E
Sbjct: 122 APFWKREATPEGDRWVE 138
>pir||B82251 molybdenum cofactor biosynthesis protein E VC1028 [imported] -
Vibrio cholerae (group O1 strain N16961)
>gi|9655491|gb|AAF94187.1| (AE004184) molybdenum
cofactor biosynthesis protein E [Vibrio cholerae]
Length = 150
Score = 104 bits (258), Expect = 4e-22
Identities = 58/136 (42%), Positives = 82/136 (59%), Gaps = 3/136 (2%)
Query: 5 KVSLVKKPEDFNI-EEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKE 63
+VS+ K EDF++ +E AL S G +V F+GKVRD NLG V L E Y M K
Sbjct: 4 RVSVQK--EDFSVAQEYEALAQGSQAGAVVTFVGKVRDMNLGDNVVGLHLEHYPGMTEKS 61
Query: 64 MERIRNEALDRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKRV 123
+ I + A +R+P+ + HRIG++ G+ +LV VS+ HR AF AC +I+D +K R
Sbjct: 62 LLEICDMAQERWPLQRVRVIHRIGDMLSGDQIVLVGVSSAHRNAAFAACEFIMDYLKTRA 121
Query: 124 PVWKREVTDEGEFWIE 139
P WK+E+T E WI+
Sbjct: 122 PFWKKELTTEASRWID 137
>pir||B69659 molybdopterin biosynthesis protein E chain [similarity] - Bacillus
subtilis >gi|2633801|emb|CAB13303.1| (Z99111)
molybdopterin converting factor (subunit 2) [Bacillus
subtilis] >gi|3282114|gb|AAC24904.1| (AF012285)
molybdopterin converting factor, subunit 2 [Bacillus
subtilis]
Length = 157
Score = 102 bits (253), Expect = 2e-21
Identities = 50/138 (36%), Positives = 84/138 (60%), Gaps = 3/138 (2%)
Query: 5 KVSLVKKPEDFNIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEM 64
+ + K P N E I V G I F+G VR+ G++ +L YEAY+ MA++ +
Sbjct: 3 RFEITKTP--INTENIIKKVEKREAGAITTFIGTVREWTNGKRTVRLEYEAYEPMAVQML 60
Query: 65 ERIRNEALDRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVP 124
+I E +++ A I HRIG L++GE +++ VS+ HR+ A+EA + ++ +K+ VP
Sbjct: 61 AQIGAEIEEKWEGASAAITHRIGVLDIGEAAVVIAVSSPHRKAAYEANEYAIERIKQIVP 120
Query: 125 VWKREVTDEGEFWIEGDR 142
+WK+E+ ++GE WI GD+
Sbjct: 121 IWKKEIWEDGEQWI-GDQ 137
>gb|AAD39471.1|AF138285_1 (AF138285) molybdopterin synthase large subunit CnxH [Aspergillus
nidulans]
Length = 195
Score = 100 bits (246), Expect = 1e-20
Identities = 52/121 (42%), Positives = 76/121 (61%), Gaps = 1/121 (0%)
Query: 20 AIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEMERIRNEALDRFPIVD 79
A+ VSS S+G ++FLG RD R V +L Y +Y +A+K + +I +EA+++F +
Sbjct: 57 ALQHVSSPSSGANILFLGTTRDTFDDRPVARLSYTSYPALALKSLHKISSEAVEKFGLNG 116
Query: 80 ALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKR-EVTDEGEFWI 138
I HR+GE+ VGE +I+V V A HR EA+ W+++ VK+RV VWKR E D G W
Sbjct: 117 VYIAHRLGEVPVGEASIVVAVGAGHRGEAWRGAEWVLEVVKERVEVWKREEFVDGGMEWR 176
Query: 139 E 139
E
Sbjct: 177 E 177
>gb|AAB64030.1| (AC002333) putative molybdopterin synthase large subunit
[Arabidopsis thaliana] >gi|4469121|emb|CAB38428.1|
(AJ133519) molybdopterin synthase large subunit
[Arabidopsis thaliana] >gi|6899949|emb|CAB71291.1|
(AJ271728) molybdopterin synthase, large subunit
[Arabidopsis thaliana]
Length = 198
Score = 97.1 bits (238), Expect = 9e-20
Identities = 48/137 (35%), Positives = 78/137 (56%)
Query: 3 KSKVSLVKKPEDFNIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIK 62
K+ + ++++ ++ + I VS+ G I F G RD G+ V +L YEAY MA +
Sbjct: 6 KNLIEILEEGHKVDVVKYIDYVSAPQAGAIATFSGTTRDMFEGKTVLELRYEAYVPMATR 65
Query: 63 EMERIRNEALDRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKR 122
+ I A + I + HR+G + VGE ++L+ VS+ HR + +AC +++DE+K
Sbjct: 66 CLSSICTTARSTWDIHKIAVAHRLGPVPVGETSVLIAVSSVHRADGLDACKFLIDELKAS 125
Query: 123 VPVWKREVTDEGEFWIE 139
VP+WK+EV GE W E
Sbjct: 126 VPIWKKEVYTNGEIWKE 142
>pir||B83156 molybdopterin converting factor, large subunit PA3916 [imported] -
Pseudomonas aeruginosa (strain PAO1)
>gi|9950100|gb|AAG07303.1|AE004809_3 (AE004809)
molybdopterin converting factor, large subunit
[Pseudomonas aeruginosa]
Length = 150
Score = 97.1 bits (238), Expect = 9e-20
Identities = 49/111 (44%), Positives = 69/111 (62%)
Query: 30 GGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEMERIRNEALDRFPIVDALIWHRIGEL 89
G +V F+G VRD N GR+V + E Y M K + +I EA R+P++ I HRIG L
Sbjct: 27 GAVVGFVGYVRDFNDGREVGGMFLEHYPGMTEKALGKIAAEAGQRWPLLRLEILHRIGRL 86
Query: 90 EVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKREVTDEGEFWIEG 140
E GE + V ++ HR+ AF+AC +++D +K R P WK+E T EG W+EG
Sbjct: 87 EPGEPIVFVGCASAHRQAAFDACNFVMDYLKTRAPFWKKEDTAEGPRWVEG 137
>gi|4758732 molybdenum cofactor synthesis 2 [Homo sapiens]
>gi|11417057|ref|XP_003912.1| molybdenum cofactor
synthesis 2 [Homo sapiens] >gi|4235631|gb|AAD13297.1|
(AF117815) molybdopterin synthase large subunit [Homo
sapiens] >gi|4262373|gb|AAD14599.1| (AF091871)
molybdopterin-synthase large subunit [Homo sapiens]
>gi|7688675|gb|AAF67478.1|AF155659_1 (AF155659)
molybdenum cofactor biosysthesis protein E [Homo
sapiens]
Length = 188
Score = 96.7 bits (237), Expect = 1e-19
Identities = 47/135 (34%), Positives = 80/135 (58%)
Query: 3 KSKVSLVKKPEDFNIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIK 62
KSK + E +++E LV S G I +F+G R+ G+KV L YEAY MA
Sbjct: 42 KSKDVINFTAEKLSVDEVSQLVISPLCGAISLFVGTTRNNFEGKKVISLEYEAYLPMAEN 101
Query: 63 EMERIRNEALDRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKR 122
E+ +I ++ ++P+ ++HR+G + V E +I++ VS+ HR + EA ++ +D +K +
Sbjct: 102 EVRKICSDIRQKWPVKHIAVFHRLGLVPVSEASIIIAVSSAHRAASLEAVSYAIDTLKAK 161
Query: 123 VPVWKREVTDEGEFW 137
VP+WK+E+ +E W
Sbjct: 162 VPIWKKEIYEESSTW 176
>sp|P45308|MOAE_HAEIN MOLYBDOPTERIN [MPT] CONVERTING FACTOR, SUBUNIT 2 (MOLYBDENUM
COFACTOR BIOSYNTHESIS PROTEIN E) (MOLYBDOPTERIN
CONVERTING FACTOR LARGE SUBUNIT) >gi|1075036|pir||H64135
molybdopterin biosynthesis protein E chain [similarity]
- Haemophilus influenzae (strain Rd KW20)
>gi|1574523|gb|AAC23318.1| (U32840) molybdopterin
converting factor, subunit 2 (moaE) [Haemophilus
influenzae Rd]
Length = 150
Score = 96.0 bits (235), Expect = 2e-19
Identities = 50/117 (42%), Positives = 70/117 (59%)
Query: 23 LVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEMERIRNEALDRFPIVDALI 82
L S G VIF+GKVRD NLG +V L E Y M K + I +A R+ I +
Sbjct: 22 LSEQHSIGATVIFVGKVRDLNLGDEVSSLYLEHYPAMTEKALNEIVAQAKVRWDIQRVSV 81
Query: 83 WHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKREVTDEGEFWIE 139
HR+G L+ G+ +LV VS+ HR +A+ A +I+D +K + P WK+E T++GE WIE
Sbjct: 82 IHRVGLLQTGDEIVLVGVSSAHRGDAYHANEFIMDFLKSKAPFWKKEQTNQGERWIE 138
>dbj|BAB06738.1| (AP001517) molybdopterin converting factor (subunit 2) [Bacillus
halodurans]
Length = 156
Score = 96.0 bits (235), Expect = 2e-19
Identities = 48/142 (33%), Positives = 84/142 (58%), Gaps = 3/142 (2%)
Query: 1 MGKSKVSLVKKPEDFNIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMA 60
M + +++++P I++ I V G I F+G VR+ G++ L YEAY MA
Sbjct: 1 MTSKRFAIMEQP--IVIQQVIDQVVHPHAGAINTFIGTVRELTKGKRTLYLQYEAYPSMA 58
Query: 61 IKEMERIRNEALDRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVK 120
K++ +I E +R+P I HRIG LE+ + +++ VS HR +++EA + ++ +K
Sbjct: 59 EKKLAQIGQEIQERWPEARVAITHRIGRLEITDVAVVIAVSTPHRADSYEASRYAIERIK 118
Query: 121 KRVPVWKREVTDEGEFWIEGDR 142
+ VP+WK+E ++GE W+ GD+
Sbjct: 119 EIVPIWKKEHWEDGEMWV-GDQ 139
>pir||B70844 molybdopterin biosynthesis protein D/E Rv3323c [similarity] -
Mycobacterium tuberculosis (strain H37RV)
>gi|2894233|emb|CAA17095.1| (AL021841) gphA
[Mycobacterium tuberculosis]
Length = 221
Score = 95.6 bits (234), Expect = 3e-19
Identities = 49/137 (35%), Positives = 75/137 (53%)
Query: 2 GKSKVSLVKKPEDFNIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAI 61
G S V E +++E + +S S GG VIF+G VR+ N G +V KL YEAY M
Sbjct: 81 GGSDVYCRLTDEPLSVDEVLNAISGPSQGGAVIFVGTVRNNNNGHEVTKLYYEAYPAMVH 140
Query: 62 KEMERIRNEALDRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKK 121
+ + I E + V + HR GEL +G+ +++ SA HR AF+A ++ +K+
Sbjct: 141 RTLMDIIEECERQADGVRVAVAHRTGELRIGDAAVVIGASAPHRAAAFDAARMCIERLKQ 200
Query: 122 RVPVWKREVTDEGEFWI 138
VP+WK+E +G W+
Sbjct: 201 DVPIWKKEFALDGVEWV 217
>emb|CAC01331.1| (AL390968) moaE-like protein [Streptomyces coelicolor A3(2)]
Length = 152
Score = 94.4 bits (231), Expect = 6e-19
Identities = 44/124 (35%), Positives = 74/124 (59%), Gaps = 2/124 (1%)
Query: 16 NIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEMERIRNEALDRF 75
+++E V ++GG +F+G VR+ + G V++L Y + A EM RI + + +
Sbjct: 28 SVDEVFGAVGDDASGGTALFVGTVRNHDGGTDVDRLGYSCHPS-AEAEMRRIAEKVVAEY 86
Query: 76 PIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKREVTDEG- 134
P+ HR+G+LEVG+ ++V VS HR EAF+AC ++D++K VP+WK + +G
Sbjct: 87 PVRALAALHRVGDLEVGDLAVVVAVSCPHRGEAFDACRKLIDDLKHEVPIWKHQTFSDGT 146
Query: 135 EFWI 138
E W+
Sbjct: 147 EEWV 150
>gb|AAC83141.1| (AF109295) MoaE [Staphylococcus carnosus]
Length = 150
Score = 93.2 bits (228), Expect = 1e-18
Identities = 43/113 (38%), Positives = 72/113 (63%)
Query: 30 GGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEMERIRNEALDRFPIVDALIWHRIGEL 89
G +V+F G VR+ G + E L YEAY MA K++ +I +E +++P I HRIG L
Sbjct: 26 GAVVVFTGHVREWTKGIRTEHLEYEAYIPMAEKKLAQIGDEINEQWPGTIVSIVHRIGPL 85
Query: 90 EVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKREVTDEGEFWIEGDR 142
++ + +L+ VS+ HR++A+ A + +D +K+ VP+WK+E+ ++G WI R
Sbjct: 86 KISDIAVLIAVSSPHRKDAYAANEYAIDRIKEVVPIWKKEIWEDGAEWIGHQR 138
>sp|Q55371|Y903_SYNY3 HYPOTHETICAL 16.5 KD PROTEIN SLR0903 >gi|7449542|pir||S75719
molybdopterin biosynthesis protein E chain [similarity]
- Synechocystis sp. (strain PCC 6803)
>gi|1001213|dbj|BAA10454.1| (D64003) molybdopterin (MPT)
converting factor, subunit 2 [Synechocystis sp.]
Length = 145
Score = 91.7 bits (224), Expect = 4e-18
Identities = 41/117 (35%), Positives = 71/117 (60%)
Query: 13 EDFNIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEMERIRNEAL 72
+ N + I+ + G +V F G VR+ N G++V+ L Y+ Y E+AI E +I EA
Sbjct: 11 QPINTQPLISALQCDQAGALVTFAGWVRNHNDGKQVDSLEYQVYRELAINEGFKIIAEAK 70
Query: 73 DRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKRE 129
+++ + A+ HR G L++GE + V A HR+ AF+ +++D++K R+P+WK+E
Sbjct: 71 EKYDLHQAIAVHRSGHLKIGETAVWVGTVASHRQAAFQGTQYVIDQIKLRLPIWKKE 127
>gi|11499761 molybdopterin converting factor, subunit 2 (moaE) [Archaeoglobus
fulgidus] >gi|7483875|pir||C69522 molybdopterin
converting factor, subunit 2 (moaE) homolog -
Archaeoglobus fulgidus >gi|2648344|gb|AAB89071.1|
(AE000954) molybdopterin converting factor, subunit 2
(moaE) [Archaeoglobus fulgidus]
Length = 239
Score = 88.2 bits (215), Expect = 4e-17
Identities = 42/109 (38%), Positives = 67/109 (60%)
Query: 30 GGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEMERIRNEALDRFPIVDALIWHRIGEL 89
G I IF+G VR E+ G+ V +L YEAYDEM ++ I IV+A ++H+ G L
Sbjct: 123 GAIGIFIGFVRKESDGKTVSRLEYEAYDEMLNEKAREIEERVKQMPGIVNARVYHKRGIL 182
Query: 90 EVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKREVTDEGEFWI 138
GE+ + + V +HR++ +E V+ +KK +P+WK+EV ++GE W+
Sbjct: 183 LPGEDIVYITVMGEHRKDIWEPLKEAVELMKKELPIWKKEVYEDGEVWV 231
>gi|7305279 molybdenum cofactor synthesis 2 [Mus musculus]
>gi|4262376|gb|AAD14601.1| (AF091872)
molybdopterin-synthase large subunit [Mus musculus]
Length = 170
Score = 87.4 bits (213), Expect = 7e-17
Identities = 45/138 (32%), Positives = 78/138 (55%), Gaps = 8/138 (5%)
Query: 2 GKSKVSLVKKPEDF--------NIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIY 53
GK + +KP+D ++ E LV S G + +F+G R+ G+KV L Y
Sbjct: 33 GKDVDDVQEKPKDIIQFTAEKLSVGEVSQLVVSPLCGAVSLFVGTTRNNFEGKKVISLEY 92
Query: 54 EAYDEMAIKEMERIRNEALDRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACA 113
EAY MA E+ +I N+ ++P+ ++HR+G + V E + ++ VS+ HR + EA +
Sbjct: 93 EAYVPMAENEIRKICNDIRQKWPVRHIAVFHRLGLVPVSEASTVIAVSSAHRAASLEAVS 152
Query: 114 WIVDEVKKRVPVWKREVT 131
+ +D +K +VP+WK+ +T
Sbjct: 153 YAIDSLKAKVPIWKKGLT 170
>gb|AAC44004.1| (U29587) homologous to moaE gene of E. coli; Method: conceptual
translation supplied by author [Rhodobacter sphaeroides]
Length = 146
Score = 85.8 bits (209), Expect = 2e-16
Identities = 48/134 (35%), Positives = 71/134 (52%), Gaps = 2/134 (1%)
Query: 5 KVSLVKKPEDFNIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEM 64
+V + +P +F E ++ G IV F G VRD L +E + E Y M + +
Sbjct: 2 RVLVQAEPFEFGAEAQTFADGAAGAGAIVTFTGLVRD--LSGALEAMEIEHYPGMTERAI 59
Query: 65 ERIRNEALDRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVP 124
I EA R+ ++DAL+ HR G L E ++V +A HR EAF A +++D +K R P
Sbjct: 60 AAIAEEARQRWHLIDALVIHRHGRLGPSEPIMMVATAAAHRAEAFAAAEFLMDYLKSRAP 119
Query: 125 VWKREVTDEGEFWI 138
WK+EVT G W+
Sbjct: 120 FWKKEVTAGGADWV 133
>pir||E75252 molybdopterin biosynthesis protein D/E DR2607 [similarity] -
Deinococcus radiodurans (strain R1)
>gi|6460436|gb|AAF12145.1|AE002090_1 (AE002090)
molybdenum cofactor biosynthesis protein D/E
[Deinococcus radiodurans]
Length = 229
Score = 85.0 bits (207), Expect = 4e-16
Identities = 44/128 (34%), Positives = 68/128 (52%)
Query: 13 EDFNIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEMERIRNEAL 72
+ ++ EA A + G F+G VR N G+ VE + YEA+ MA K M A
Sbjct: 95 DPLSLSEADAFLVKPEYGAQAYFMGTVRSPNQGQVVEYIDYEAFAPMAEKVMREAAALAR 154
Query: 73 DRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKREVTD 132
+R + I HR G L +I++ V++ HRR A EAC ++++ +K +P+WK E
Sbjct: 155 ERHGELRVWIEHRTGRLTPAVASIVIGVASPHRRPALEACDFLIEHLKIELPIWKHEADG 214
Query: 133 EGEFWIEG 140
GE W++G
Sbjct: 215 RGEHWVKG 222
>pir||B70816 probable molybdopterin biosynthesis protein E2 chain [similarity] -
Mycobacterium tuberculosis (strain H37RV)
>gi|2916925|emb|CAA17672.1| (AL022004) moaE2
[Mycobacterium tuberculosis]
Length = 141
Score = 82.7 bits (201), Expect = 2e-15
Identities = 45/117 (38%), Positives = 67/117 (56%), Gaps = 1/117 (0%)
Query: 23 LVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEMERIRNEALDRFPIVDALI 82
LVS S G IV F+G +RD + GR V +L Y A+ A + + A + +
Sbjct: 22 LVSHRSAGAIVGFVGMIRDRDGGRGVLRLEYSAHPSAAQVLADLVAEVAEESSGVRAVAA 81
Query: 83 WHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKREVTDEG-EFWI 138
HRIG L+VGE ++ V+A HRR AF CA +V+ +K R+PVWK + ++G + W+
Sbjct: 82 SHRIGVLQVGEAALVAAVAADHRRAAFGTCAHLVETIKARLPVWKHQFFEDGTDEWV 138
>pir||T44853 molybdopterin biosynthesis protein E chain [similarity] -
Arthrobacter nicotinovorans plasmid pAO1
>gi|2288878|emb|CAA71782.1| (Y10817) molybdopterin
synthase, large subunit [Arthrobacter nicotinovorans]
Length = 155
Score = 81.9 bits (199), Expect = 3e-15
Identities = 45/133 (33%), Positives = 76/133 (56%), Gaps = 14/133 (10%)
Query: 13 EDFNIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAY--------DEM-AIKE 63
+ +++ AIA V S ++G +V F G VRD + G+ V++L Y A+ D M A+
Sbjct: 15 QPISVDVAIAAVDSRTSGAVVSFFGVVRDHDGGKAVDRLSYTAHPTAHQALSDVMEAVAA 74
Query: 64 MERIRNEALDRFPIVDALIW--HRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKK 121
+EA + P+ IW HR+G L++G+ ++ V+A HR +AF C+ +VD +K+
Sbjct: 75 KYSFSDEAEEGVPV---RIWAAHRVGALQIGQAALVCAVAAGHRGQAFAVCSELVDRIKE 131
Query: 122 RVPVWKREVTDEG 134
VP+WK + +G
Sbjct: 132 EVPIWKEQFFSDG 144
>sp|Q56210|MOAE_SYNP7 MOLYBDOPTERIN [MPT] CONVERTING FACTOR, SUBUNIT 2 (MOLYBDENUM
COFACTOR BIOSYNTHESIS PROTEIN E) (MOLYBDOPTERIN
CONVERTING FACTOR LARGE SUBUNIT)
>gi|1480153|emb|CAA67947.1| (X99625) moaE [Synechococcus
sp.]
Length = 165
Score = 79.2 bits (192), Expect = 2e-14
Identities = 41/114 (35%), Positives = 66/114 (56%), Gaps = 2/114 (1%)
Query: 30 GGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEMERIRNEALDRF-PIVDALIWHRIGE 88
G F+G VR N+GR V + Y+++ + + I EA F + + HR+GE
Sbjct: 34 GAFASFVGWVRRVNVGRLVTGITYQSFQPLCRTVLTEICQEAEQVFGQELRIYVQHRLGE 93
Query: 89 LEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKREVTDEGEF-WIEGD 141
VG+ T+L+ V A HR EA EAC ++++E+K R P+WK E ++G+ W+ G+
Sbjct: 94 TRVGDPTVLIGVGAIHRDEACEACRYVIEELKHRAPIWKLEHYEDGDSGWVPGN 147
>pir||T25919 hypothetical protein T27A3.6 - Caenorhabditis elegans
>gi|1825695|gb|AAB42299.1| (U88180) similar to
molybdenum cofactor biosynthesis protein E
[Caenorhabditis elegans]
Length = 340
Score = 78.8 bits (191), Expect = 3e-14
Identities = 42/124 (33%), Positives = 71/124 (56%), Gaps = 1/124 (0%)
Query: 13 EDFNIEEAIALVSSSSTGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEMERIRNEAL 72
E +++ LVS S G I F G RD + GR V L Y+ +D MA K++ I E
Sbjct: 203 EVIDVKVVDQLVSHPSCGAISTFNGVTRDNHGGRDVSNLSYDCHDLMAYKKLRGICAEIR 262
Query: 73 DRFPIVDAL-IWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKREVT 131
+P V + I+HR+G++++GE+++++ S+ HR+ A +A +D +K+ P++K E
Sbjct: 263 AEYPDVKKIAIFHRLGKVDIGESSVIISTSSPHRKTAIQATEKCIDLLKEHAPIFKYEEY 322
Query: 132 DEGE 135
GE
Sbjct: 323 SNGE 326
>pir||E72646 probable molybdopterin (mpt) converting factor, subunit 2 APE0603 -
Aeropyrum pernix (strain K1) >gi|5104258|dbj|BAA79573.1|
(AP000060) 249aa long hypothetical molybdopterin (mpt)
converting factor, subunit 2 [Aeropyrum pernix]
Length = 249
Score = 76.5 bits (185), Expect = 1e-13
Identities = 48/140 (34%), Positives = 74/140 (52%), Gaps = 3/140 (2%)
Query: 8 LVKKPEDFNIEEAIALVSSSS--TGGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEME 65
L+K E +I +A +S+S G + +F+G V+ G +VEKL YEA +A + +E
Sbjct: 98 LLKPGEPVDIASLVARAASASPANGAVAVFVGTVKSVVGGVRVEKLFYEAAWGVAERVIE 157
Query: 66 RIRNEALDRFPIVDALIWHRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPV 125
I E + A+++H GE GE TI+ VV+ R + +VD VK+ P+
Sbjct: 158 SILREEAGANGLSAAIVYHYTGERRPGETTIVAVVAGASRGNVYPGLQRVVDRVKREAPI 217
Query: 126 WKREVTDEG-EFWIEGDRHV 144
WK E + G + +I GDR V
Sbjct: 218 WKVEYREGGVKAYILGDRVV 237
>gb|AAG18716.1| (AE004976) molybdenum cofactor biosynthesis protein; MoaE
[Halobacterium sp. NRC-1]
Length = 297
Score = 74.5 bits (180), Expect = 5e-13
Identities = 41/114 (35%), Positives = 63/114 (54%), Gaps = 2/114 (1%)
Query: 26 SSSTGGIVIFLGKVR--DENLGRKVEKLIYEAYDEMAIKEMERIRNEALDRFPIVDALIW 83
S G I F G+VR D+ + L +E YD +A + M IR + +R + D +
Sbjct: 178 SEFAGAIATFTGRVRERDDPDDTPTQHLEFERYDTVAEQRMATIREQLCERDGVYDVRMH 237
Query: 84 HRIGELEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKREVTDEGEFW 137
HR G + G++ + VVV A HR +AF A + +D +K VP++K+EVT + FW
Sbjct: 238 HRTGVVPAGDDVVHVVVLAGHRGDAFRAVSDGIDRLKDEVPLFKKEVTVDDAFW 291
>gb|AAK42541.1| Molybdenum cofactor biosynthesis protein E (moaE) [Sulfolobus
solfataricus]
Length = 231
Score = 70.2 bits (169), Expect = 1e-11
Identities = 34/110 (30%), Positives = 64/110 (57%), Gaps = 1/110 (0%)
Query: 30 GGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEMERIRNEALDRF-PIVDALIWHRIGE 88
G +V+++G V+ +V +L YEAY+E K I++E ++ +++ I H I
Sbjct: 112 GSLVVYVGFVKGIVDNHRVFELKYEAYEEYTRKRFLEIKDEIKKKYNDLIEIEIIHVIDS 171
Query: 89 LEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKREVTDEGEFWI 138
++ GEN +L++ K R++A +A ++ VK +WK E+ D+GE+W+
Sbjct: 172 MKPGENVLLIMAIGKGRKDAIDAVKETLELVKHSTGIWKLEIRDDGEYWV 221
>gb|AAF76450.1|AF077841_1 (AF077841) putative MoaD2 [Sulfolobus solfataricus]
Length = 231
Score = 70.2 bits (169), Expect = 1e-11
Identities = 34/110 (30%), Positives = 64/110 (57%), Gaps = 1/110 (0%)
Query: 30 GGIVIFLGKVRDENLGRKVEKLIYEAYDEMAIKEMERIRNEALDRF-PIVDALIWHRIGE 88
G +V+++G V+ +V +L YEAY+E K I++E ++ +++ I H I
Sbjct: 112 GSLVVYVGFVKGIVDNHRVFELKYEAYEEYTRKRFLEIKDEIKKKYNDLIEIEIIHVIDS 171
Query: 89 LEVGENTILVVVSAKHRREAFEACAWIVDEVKKRVPVWKREVTDEGEFWI 138
++ GEN +L++ K R++A +A ++ VK +WK E+ D+GE+W+
Sbjct: 172 MKPGENVLLIMAIGKGRKDAIDAVKETLELVKHSTGIWKLEIRDDGEYWV 221
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.319 0.138 0.400
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 54383957
Number of Sequences: 2977
Number of extensions: 1995529
Number of successful extensions: 5651
Number of sequences better than 1.0e-10: 30
Number of HSP's better than 0.0 without gapping: 24
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 5619
Number of HSP's gapped (non-prelim): 30
length of query: 148
length of database: 189,106,746
effective HSP length: 53
effective length of query: 95
effective length of database: 157,386,935
effective search space: 14951758825
effective search space used: 14951758825
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 161 (67.1 bits)