BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0139 (lysS) DE:lysyl-tRNA synthetase (lysS)
(526 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9V270|SYK_PYRAB LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) ... 1097 0.0
sp|O57963|SYK_PYRHO LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) ... 965 0.0
sp|O83650|SYK_TREPA LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) ... 402 e-111
pir||C64367 hypothetical protein MJ0539 - Methanococcus jannaschii 382 e-105
sp|Q57959|SYK_METJA LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) ... 381 e-104
sp|O51603|SYK_BORBU LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) ... 380 e-104
sp|O30522|SYK_METMP LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) ... 353 4e-96
gi|11498815 lysyl-tRNA synthetase (lysS) [Archaeoglobus fulgidus... 333 3e-90
sp|O27585|SYK_METTH LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) ... 332 8e-90
sp|P57677|SYK_THEAC LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) ... 298 8e-80
gb|AAG20183.1| (AE005095) lysyl-tRNA synthetase; LysS [Halobacte... 267 2e-70
sp|Q9X895|SYK_STRCO LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) ... 252 5e-66
sp|Q9YFT9|SYK_AERPE LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) ... 229 8e-59
sp|O74059|SYK_CERSY LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) ... 186 6e-46
sp|Q9ZDF8|SYK_RICPR LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) ... 150 3e-35
>sp|Q9V270|SYK_PYRAB LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) (LYSRS)
>gi|7450808|pir||A75210 lysyl-tRNA synthetase (lyss)
PAB0139 - Pyrococcus abyssi (strain Orsay)
>gi|5457637|emb|CAB49128.1| (AJ248283) lysyl-tRNA
synthetase (lysS) [Pyrococcus abyssi]
Length = 526
Score = 1097 bits (2807), Expect = 0.0
Identities = 526/526 (100%), Positives = 526/526 (100%)
Query: 1 MVIVVHWADYVADKIIRERGEKDRYVVESGITPSGYVHVGNFRELFTAYIVGHALRDKGY 60
MVIVVHWADYVADKIIRERGEKDRYVVESGITPSGYVHVGNFRELFTAYIVGHALRDKGY
Sbjct: 1 MVIVVHWADYVADKIIRERGEKDRYVVESGITPSGYVHVGNFRELFTAYIVGHALRDKGY 60
Query: 61 EVRHIHMWDDYDRFRKVPRNVPPEWKEYLGMPVSEVPDPWGCHDSYAEHFMEKFEREVKE 120
EVRHIHMWDDYDRFRKVPRNVPPEWKEYLGMPVSEVPDPWGCHDSYAEHFMEKFEREVKE
Sbjct: 61 EVRHIHMWDDYDRFRKVPRNVPPEWKEYLGMPVSEVPDPWGCHDSYAEHFMEKFEREVKE 120
Query: 121 LGIEVEFLYASELYKRGEYSEEIRKAFEGKEKIMEILNKYREIAKQPPLPENWWPAMVYC 180
LGIEVEFLYASELYKRGEYSEEIRKAFEGKEKIMEILNKYREIAKQPPLPENWWPAMVYC
Sbjct: 121 LGIEVEFLYASELYKRGEYSEEIRKAFEGKEKIMEILNKYREIAKQPPLPENWWPAMVYC 180
Query: 181 PEHRKEAEIIEWDGEWKVKYRCPEGHEGWVDIRSGNVKLRWRVDWPMRWAHFDVDFEPAG 240
PEHRKEAEIIEWDGEWKVKYRCPEGHEGWVDIRSGNVKLRWRVDWPMRWAHFDVDFEPAG
Sbjct: 181 PEHRKEAEIIEWDGEWKVKYRCPEGHEGWVDIRSGNVKLRWRVDWPMRWAHFDVDFEPAG 240
Query: 241 KDHLAAGSSYDTGKEIIKAIYGKEAPMSLMYEFVGIKGEKGKMSGSKGNVILLSDLYEVL 300
KDHLAAGSSYDTGKEIIKAIYGKEAPMSLMYEFVGIKGEKGKMSGSKGNVILLSDLYEVL
Sbjct: 241 KDHLAAGSSYDTGKEIIKAIYGKEAPMSLMYEFVGIKGEKGKMSGSKGNVILLSDLYEVL 300
Query: 301 EPGLVRFIYARHRPNKEIRIDLGMGLLNLYDEFDKVERIYFGVEDAKGDVEELKRTYELS 360
EPGLVRFIYARHRPNKEIRIDLGMGLLNLYDEFDKVERIYFGVEDAKGDVEELKRTYELS
Sbjct: 301 EPGLVRFIYARHRPNKEIRIDLGMGLLNLYDEFDKVERIYFGVEDAKGDVEELKRTYELS 360
Query: 361 NPRIPNRLIAQAPFRFLAVLVQMPHLDEDGIISVLTNQGHVPKELEREDIERIRLRIRLA 420
NPRIPNRLIAQAPFRFLAVLVQMPHLDEDGIISVLTNQGHVPKELEREDIERIRLRIRLA
Sbjct: 361 NPRIPNRLIAQAPFRFLAVLVQMPHLDEDGIISVLTNQGHVPKELEREDIERIRLRIRLA 420
Query: 421 KNWVEKYAPDSVKFSILPEPPKIEIDESVREAMEEVAKWIESKSTFSVDELNNVLFEVAK 480
KNWVEKYAPDSVKFSILPEPPKIEIDESVREAMEEVAKWIESKSTFSVDELNNVLFEVAK
Sbjct: 421 KNWVEKYAPDSVKFSILPEPPKIEIDESVREAMEEVAKWIESKSTFSVDELNNVLFEVAK 480
Query: 481 KRGISSKEWFSTLYKLFIGKERGPRLASFLASLDKDFVVRRLRLEG 526
KRGISSKEWFSTLYKLFIGKERGPRLASFLASLDKDFVVRRLRLEG
Sbjct: 481 KRGISSKEWFSTLYKLFIGKERGPRLASFLASLDKDFVVRRLRLEG 526
>sp|O57963|SYK_PYRHO LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) (LYSRS)
>gi|7450803|pir||A71246 lysine--tRNA ligase (EC 6.1.1.6)
- Pyrococcus horikoshii >gi|3256613|dbj|BAA29296.1|
(AP000001) 523aa long hypothetical lysyl-tRNA synthetase
[Pyrococcus horikoshii]
Length = 523
Score = 965 bits (2467), Expect = 0.0
Identities = 440/523 (84%), Positives = 492/523 (93%)
Query: 4 VVHWADYVADKIIRERGEKDRYVVESGITPSGYVHVGNFRELFTAYIVGHALRDKGYEVR 63
+VHWADY+ADKIIRERGEK++YVVESGITPSGYVHVGNFRELFTAYIVGHALRDKGYEVR
Sbjct: 1 MVHWADYIADKIIRERGEKEKYVVESGITPSGYVHVGNFRELFTAYIVGHALRDKGYEVR 60
Query: 64 HIHMWDDYDRFRKVPRNVPPEWKEYLGMPVSEVPDPWGCHDSYAEHFMEKFEREVKELGI 123
HIHMWDDYDRFRKVPRNVP EWK+YLGMP+SEVPDPWGCH+SYAEHFM KFE EV++LGI
Sbjct: 61 HIHMWDDYDRFRKVPRNVPQEWKDYLGMPISEVPDPWGCHESYAEHFMRKFEEEVEKLGI 120
Query: 124 EVEFLYASELYKRGEYSEEIRKAFEGKEKIMEILNKYREIAKQPPLPENWWPAMVYCPEH 183
EV+FLYASELYKRGEYSEEIR AFE ++KIMEILNKYREIAKQPPLPENWWPAMVYCPEH
Sbjct: 121 EVDFLYASELYKRGEYSEEIRLAFEKRDKIMEILNKYREIAKQPPLPENWWPAMVYCPEH 180
Query: 184 RKEAEIIEWDGEWKVKYRCPEGHEGWVDIRSGNVKLRWRVDWPMRWAHFDVDFEPAGKDH 243
R+EAEIIEWDG WKVKY+CPEGHEGWVDIRSGNVKLRWRVDWPMRW+HF VDFEPAGKDH
Sbjct: 181 RREAEIIEWDGGWKVKYKCPEGHEGWVDIRSGNVKLRWRVDWPMRWSHFGVDFEPAGKDH 240
Query: 244 LAAGSSYDTGKEIIKAIYGKEAPMSLMYEFVGIKGEKGKMSGSKGNVILLSDLYEVLEPG 303
L AGSSYDTGKEIIK +YGKEAP+SLMYEFVGIKG+KGKMSGSKGNVILLSDLYEVLEPG
Sbjct: 241 LVAGSSYDTGKEIIKEVYGKEAPLSLMYEFVGIKGQKGKMSGSKGNVILLSDLYEVLEPG 300
Query: 304 LVRFIYARHRPNKEIRIDLGMGLLNLYDEFDKVERIYFGVEDAKGDVEELKRTYELSNPR 363
LVRFIYARHRPNKEI+IDLG+G+LNLYDEFDKVERIYFGVE KGD EEL+RTYELS P+
Sbjct: 301 LVRFIYARHRPNKEIKIDLGLGILNLYDEFDKVERIYFGVEGGKGDDEELRRTYELSMPK 360
Query: 364 IPNRLIAQAPFRFLAVLVQMPHLDEDGIISVLTNQGHVPKELEREDIERIRLRIRLAKNW 423
P RL+AQAPFRFLAVLVQ+PHL E+ II+VL QGH+P++L +ED+ER++LRI LA+NW
Sbjct: 361 KPERLVAQAPFRFLAVLVQLPHLTEEDIINVLIKQGHIPRDLSKEDVERVKLRINLARNW 420
Query: 424 VEKYAPDSVKFSILPEPPKIEIDESVREAMEEVAKWIESKSTFSVDELNNVLFEVAKKRG 483
V+KYAP+ VKFSIL +PP++E+ E VREAM EVA+W+E+ FSV+E NN+LFEVAK+RG
Sbjct: 421 VKKYAPEDVKFSILEKPPEVEVSEDVREAMNEVAEWLENHEEFSVEEFNNILFEVAKRRG 480
Query: 484 ISSKEWFSTLYKLFIGKERGPRLASFLASLDKDFVVRRLRLEG 526
ISS+EWFSTLY+LFIGKERGPRLASFLASLD+ FV++RLRLEG
Sbjct: 481 ISSREWFSTLYRLFIGKERGPRLASFLASLDRSFVIKRLRLEG 523
>sp|O83650|SYK_TREPA LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) (LYSRS)
>gi|7450805|pir||C71299 lysine--tRNA ligase (EC 6.1.1.6)
(lysS-1) - syphilis spirochete
>gi|3322943|gb|AAC65618.1| (AE001239) lysyl-tRNA
synthetase (lysS-1) [Treponema pallidum]
Length = 528
Score = 402 bits (1022), Expect = e-111
Identities = 208/515 (40%), Positives = 317/515 (61%), Gaps = 18/515 (3%)
Query: 5 VHWADYVADKIIRERGEKDRYVVESGITPSGYVHVGNFRELFTAYIVGHALRDKGYEVRH 64
+HWAD VA KII+ER + D+Y SGITPSG VH+GNFRE+ + +V ALRD+G VR
Sbjct: 8 LHWADKVAHKIIKERADCDQYTCASGITPSGTVHIGNFREIISVDLVVRALRDQGKSVRF 67
Query: 65 IHMWDDYDRFRKVPRNVPP--EWKEYLGMPVSEVPDPWGCHDSYAEHFMEKFEREVKELG 122
+H WDDYD FR++P NVP E K+Y+ MP++ VPDP+ DSYA H + E + E+G
Sbjct: 68 VHSWDDYDVFRRIPDNVPAQDELKQYIRMPITSVPDPFQQEDSYARHHEREIESALPEVG 127
Query: 123 IEVEFLYASELYKRGEYSEEIRKAFEGKEKIMEILNKYREIAKQPPLPENWWPAMVYCPE 182
I E++Y S+ Y+ G Y++EI+ A + + +I ILN+YR+ +Q + +WP V+C
Sbjct: 128 IYPEYVYQSKQYQAGVYAQEIKIALDNRHRIQAILNEYRD--EQHKISGTYWPVSVFCTA 185
Query: 183 HRKEAEIIE-WDGEWKVKYRCPEGHEGWVDIRSGN-VKLRWRVDWPMRWAHFDVDFEPAG 240
K+ ++ WD W ++Y C GH VD+R + VKL WRVDW MRW+ V FEPAG
Sbjct: 186 CHKDCTTVDAWDSHWCLQYHCECGHGEQVDLRQTSAVKLSWRVDWAMRWSKEHVVFEPAG 245
Query: 241 KDHLAAGSSYDTGKEIIKAIYGKEAPMSLMYEFVGIKGEKGKMSGSKGNVILLSDLYEVL 300
KDH + G S+DT + I IY AP+S Y+F+G+KG GKMS S G V+ L D+ EV
Sbjct: 246 KDHHSQGGSFDTARLISDHIYHWPAPVSFRYDFIGLKGLPGKMSSSAGKVVGLRDVLEVY 305
Query: 301 EPGLVRFIYARHRPNKEIRIDLGMGLLNLYDEFDKVERIYFGVEDAKGDVEEL--KRTYE 358
+P ++R+++ RPN E I + +L +Y+++DK ER+ +G+ AK + E + KR YE
Sbjct: 306 QPEVLRYLFVSTRPNTEFSISFDLDVLKIYEDYDKSERVAWGIHAAKSEHEFMRHKRIYE 365
Query: 359 LSNPR-IPNRLIAQAPFRFLAVLVQMPHLDEDGIISVLTNQGHVPKELEREDIERIRLRI 417
LS R +P + Q PFR + ++Q+ D +++ + ++ ++ IER R
Sbjct: 366 LSQVRGMPPCISYQVPFRHVCNILQINSGDISAVLAFFS-------DIHKDQIERFVRRC 418
Query: 418 RLAKNWV-EKYAPDSVKFSILPEPPKIEIDESVREAMEEVAKWIESKS-TFSVDELNNVL 475
+ A NW+ + APD KF++ + ++ + + EA+ + + ++ S EL+ L
Sbjct: 419 QCAWNWIRDAGAPDDFKFTLKEDGVRVPLSAEITEALRLIRDTLVPRTDVLSEKELSAEL 478
Query: 476 FEVAKKRGISSKEWFSTLYKLFIGKERGPRLASFL 510
+ VA++ + SKE F+ LY++ IGK +GPRLA F+
Sbjct: 479 YAVARQIPVGSKELFTALYQVLIGKNQGPRLAGFM 513
>pir||C64367 hypothetical protein MJ0539 - Methanococcus jannaschii
Length = 534
Score = 382 bits (971), Expect = e-105
Identities = 208/538 (38%), Positives = 321/538 (59%), Gaps = 26/538 (4%)
Query: 4 VVHWADYVADKIIRERGEKDRYVVESGITPSGYVHVGNFRELFTAYIVGHALRDKGYEVR 63
++HWAD +A+K+I ER + D+Y+V SGITPSG++HVGN RE TA + L +KG E
Sbjct: 4 IMHWADVIAEKLIEER-KADKYIVASGITPSGHIHVGNARETLTADAIYKGLINKGVEAE 62
Query: 64 HIHMWDDYDRFRKVPRNVPPEWKEYLGMPVSEVPDPWGCHDSYAEHFMEKFEREVKELGI 123
I + D YD RK+ +P E+++Y+GMP+SE+P P GC +SYAEHF+ + + +LG+
Sbjct: 63 LIFIADTYDPLRKLYPFLPKEFEQYIGMPLSEIPCPEGCCESYAEHFLRPYLESLDDLGV 122
Query: 124 EVEFLYASELYKRGEYSEEIRKAFEGKEKIMEILNKYREIAKQPPLPENWWPAMVYCPEH 183
E+ A E YK+G Y E+I+ A + +EKIMEILNK+R PLP++WWP + C
Sbjct: 123 ELTTYRADENYKKGLYDEKIKIALDNREKIMEILNKFR----ANPLPDDWWPINIVCENC 178
Query: 184 RK-EAEIIEWDGE-WKVKYRCPE-GHEGWVDIRSGNVKLRWRVDWPMRWAHFDVDFEPAG 240
K + ++I++D E ++ YRC G E V G KL WRVDWP RW+ F+V EP G
Sbjct: 179 GKLKTKVIKYDSEKEEITYRCEICGFENTVKPYKGRAKLPWRVDWPARWSIFNVTIEPMG 238
Query: 241 KDHLAAGSSYDTGKEIIKAIYGKEAPMSLMYEFVGIK-GEKG-KMSGSKGNVILLSDLYE 298
KDH AAG SYDTG I K IY P ++YE++ +K G+K MS SKG V + D
Sbjct: 239 KDHAAAGGSYDTGVLIAKEIYNYIPPKKVVYEWIQLKVGDKAIPMSSSKGVVFAVKDWTN 298
Query: 299 VLEPGLVRFIYARHRPNKEIRIDLGMGLLNLYDEFDKVERIYFGVED---AKGDVEELKR 355
+ P ++RF+ R +P K I DL + +L DE+D++E YF +D + +E R
Sbjct: 299 IAHPEILRFLLLRSKPTKHIDFDL-KKIPDLVDEYDRLEDFYFNNKDKDELSEEEQEKIR 357
Query: 356 TYELSNPRIPNRLIAQAPFRFLAVLVQMPHLDE------DGIISVLTNQGHVPKELERED 409
YELS P+IP P+RF +++ Q+ + +E + + +L + +++
Sbjct: 358 IYELSTPKIPETKPFVIPYRFCSIIAQLTYDEEKEDINMERVFEILRRNNYSIDDIDEFS 417
Query: 410 IERIRLRIRLAKNWVEKYAPDSVKFSILPEPPKIEIDESVREAMEEVAKWIESK---STF 466
+++++ R+ +A+NW KY K I+ E EI E +++ +E K+ K + F
Sbjct: 418 MKKLKDRLLMARNWALKYGE---KLVIISEDEAKEIYEKLKDKQKEWIKYFAEKLKTAEF 474
Query: 467 SVDELNNVLFEVAKKRGISSKEWFSTLYKLFIGKERGPRLASFLASLDKDFVVRRLRL 524
L+ ++++ AK+ G++ ++ F Y + +GK+ GP+L +FLA+L KDFV+RR L
Sbjct: 475 DALNLHELIYQTAKELGLNPRDAFQASYMILLGKKYGPKLGAFLATLGKDFVIRRYSL 532
>sp|Q57959|SYK_METJA LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) (LYSRS)
>gi|2826296|gb|AAB98532.1| (U67503) tRNA synthetase,
putative [Methanococcus jannaschii]
Length = 530
Score = 381 bits (968), Expect = e-104
Identities = 208/537 (38%), Positives = 320/537 (58%), Gaps = 26/537 (4%)
Query: 5 VHWADYVADKIIRERGEKDRYVVESGITPSGYVHVGNFRELFTAYIVGHALRDKGYEVRH 64
+HWAD +A+K+I ER + D+Y+V SGITPSG++HVGN RE TA + L +KG E
Sbjct: 1 MHWADVIAEKLIEER-KADKYIVASGITPSGHIHVGNARETLTADAIYKGLINKGVEAEL 59
Query: 65 IHMWDDYDRFRKVPRNVPPEWKEYLGMPVSEVPDPWGCHDSYAEHFMEKFEREVKELGIE 124
I + D YD RK+ +P E+++Y+GMP+SE+P P GC +SYAEHF+ + + +LG+E
Sbjct: 60 IFIADTYDPLRKLYPFLPKEFEQYIGMPLSEIPCPEGCCESYAEHFLRPYLESLDDLGVE 119
Query: 125 VEFLYASELYKRGEYSEEIRKAFEGKEKIMEILNKYREIAKQPPLPENWWPAMVYCPEHR 184
+ A E YK+G Y E+I+ A + +EKIMEILNK+R PLP++WWP + C
Sbjct: 120 LTTYRADENYKKGLYDEKIKIALDNREKIMEILNKFR----ANPLPDDWWPINIVCENCG 175
Query: 185 K-EAEIIEWDGE-WKVKYRCPE-GHEGWVDIRSGNVKLRWRVDWPMRWAHFDVDFEPAGK 241
K + ++I++D E ++ YRC G E V G KL WRVDWP RW+ F+V EP GK
Sbjct: 176 KLKTKVIKYDSEKEEITYRCEICGFENTVKPYKGRAKLPWRVDWPARWSIFNVTIEPMGK 235
Query: 242 DHLAAGSSYDTGKEIIKAIYGKEAPMSLMYEFVGIK-GEKG-KMSGSKGNVILLSDLYEV 299
DH AAG SYDTG I K IY P ++YE++ +K G+K MS SKG V + D +
Sbjct: 236 DHAAAGGSYDTGVLIAKEIYNYIPPKKVVYEWIQLKVGDKAIPMSSSKGVVFAVKDWTNI 295
Query: 300 LEPGLVRFIYARHRPNKEIRIDLGMGLLNLYDEFDKVERIYFGVED---AKGDVEELKRT 356
P ++RF+ R +P K I DL + +L DE+D++E YF +D + +E R
Sbjct: 296 AHPEILRFLLLRSKPTKHIDFDL-KKIPDLVDEYDRLEDFYFNNKDKDELSEEEQEKIRI 354
Query: 357 YELSNPRIPNRLIAQAPFRFLAVLVQMPHLDE------DGIISVLTNQGHVPKELEREDI 410
YELS P+IP P+RF +++ Q+ + +E + + +L + +++ +
Sbjct: 355 YELSTPKIPETKPFVIPYRFCSIIAQLTYDEEKEDINMERVFEILRRNNYSIDDIDEFSM 414
Query: 411 ERIRLRIRLAKNWVEKYAPDSVKFSILPEPPKIEIDESVREAMEEVAKWIESK---STFS 467
++++ R+ +A+NW KY K I+ E EI E +++ +E K+ K + F
Sbjct: 415 KKLKDRLLMARNWALKYGE---KLVIISEDEAKEIYEKLKDKQKEWIKYFAEKLKTAEFD 471
Query: 468 VDELNNVLFEVAKKRGISSKEWFSTLYKLFIGKERGPRLASFLASLDKDFVVRRLRL 524
L+ ++++ AK+ G++ ++ F Y + +GK+ GP+L +FLA+L KDFV+RR L
Sbjct: 472 ALNLHELIYQTAKELGLNPRDAFQASYMILLGKKYGPKLGAFLATLGKDFVIRRYSL 528
>sp|O51603|SYK_BORBU LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) (LYSRS)
>gi|7450804|pir||B70182 lysine--tRNA ligase (EC 6.1.1.6)
- Lyme disease spirochete >gi|2688588|gb|AAC67006.1|
(AE001167) lysyl-tRNA synthetase [Borrelia burgdorferi]
Length = 521
Score = 380 bits (965), Expect = e-104
Identities = 201/521 (38%), Positives = 319/521 (60%), Gaps = 24/521 (4%)
Query: 6 HWADYVADKIIRERGEKDRYVVESGITPSGYVHVGNFRELFTAYIVGHALRDKGYEVRHI 65
HWAD+ A+KI +E+G K+ Y V SGITPSG VH+GNFRE+ + +V ALRD G +VR I
Sbjct: 5 HWADFYAEKIKKEKGPKNLYTVASGITPSGTVHIGNFREVISVDLVARALRDSGSKVRFI 64
Query: 66 HMWDDYDRFRKVPRNVPPE--WKEYLGMPVSEVPDPWGCHDSYAEHFMEKFEREVKELGI 123
+ WD+YD FRKVP+N+P + YL ++ VPD SYA +FE+ + +GI
Sbjct: 65 YSWDNYDVFRKVPKNMPEQELLTTYLRQAITRVPDTRSHKTSYARANEIEFEKYLPVVGI 124
Query: 124 EVEFLYASELYKRGEYSEEIRKAFEGKEKIMEILNKYREIAKQPPLPENWWPAMVYCPE- 182
EF+ S+ Y Y+ +I+ A + K+++ E LN+YR L ENW+P V+C +
Sbjct: 125 NPEFIDQSKQYTSNAYASQIKFALDHKKELSEALNEYR----TSKLEENWYPISVFCTKC 180
Query: 183 HRKEAEIIEWDGEWKVKYRCPEGHEGWVDIR-SGNVKLRWRVDWPMRWAHFDVDFEPAGK 241
+R + +D + V+Y C G++ +DIR + +KL WR+DWPMRW + VDFEPAGK
Sbjct: 181 NRDTTTVNNYDNHYSVEYSCECGNQESLDIRTTWAIKLPWRIDWPMRWKYEKVDFEPAGK 240
Query: 242 DHLAAGSSYDTGKEIIKAIYGKEAPMSLMYEFVGIKGEKGKMSGSKGNVILLSDLYEVLE 301
DH ++G S+DT K I+K G P++ Y+F+ IKG GK+S S G+VI L D+ EV
Sbjct: 241 DHHSSGGSFDTSKNIVKIFQG-SPPVTFQYDFISIKGRGGKISSSSGDVISLKDVLEVYT 299
Query: 302 PGLVRFIYARHRPNKEIRIDLGMGLLNLYDEFDKVERIYFGVEDAKGDVEE-LKRTYELS 360
P + RF++A +PN E I + ++ +Y+++DK ERIY+GVED K + + KR YELS
Sbjct: 300 PEVTRFLFAATKPNTEFSISFDLDVIKIYEDYDKFERIYYGVEDVKEEKKRAFKRIYELS 359
Query: 361 NPRIPNRLIA-QAPFRFLAVLVQMPHLDEDGIISVLTNQGHVPKELEREDIERIRLRIRL 419
P +P++ I Q FR L+V+ Q+ + + I++ L K ++ + +++ +I
Sbjct: 360 QPYMPSKRIPYQVGFRHLSVISQIFENNINKILNYL-------KNVQEDQKDKLINKINC 412
Query: 420 AKNWVEKYAPDSVKFSILPEPPKIEI-DESVREAMEEVAKWIESKSTFSV---DELNNVL 475
A NW+ +AP+ KFS+ + +EI +E+ ++A+ E+ ++ K F V ++ N +
Sbjct: 413 AINWIRDFAPEDFKFSLRSKFDNMEILEENSKKAINELLDFL--KKNFEVATEQDIQNEI 470
Query: 476 FEVAKKRGISSKEWFSTLYKLFIGKERGPRLASFLASLDKD 516
++++++ I +F +YK+ I KE+GP+LA F+ + D
Sbjct: 471 YKISRENNIEPALFFKQIYKILIDKEKGPKLAGFIKIIGID 511
>sp|O30522|SYK_METMP LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) (LYSRS)
>gi|11280297|pir||T46975 lysine--tRNA ligase (EC
6.1.1.6) [validated] - Methanococcus maripaludis
>gi|2645489|gb|AAB87410.1| (AF009824) lysyl-tRNA
synthetase [Methanococcus maripaludis]
Length = 533
Score = 353 bits (895), Expect = 4e-96
Identities = 201/543 (37%), Positives = 304/543 (55%), Gaps = 35/543 (6%)
Query: 5 VHWADYVADKIIRERGEKDRYVVESGITPSGYVHVGNFRELFTAYIVGHALRDKGYEVRH 64
+HWAD ++KI+++R ++ YVV SGITPSG++H+GN RE TA V + KG E +
Sbjct: 1 MHWADATSEKIMKKRNAEE-YVVSSGITPSGHIHIGNARETLTADAVYKGMLKKGAEAKL 59
Query: 65 IHMWDDYDRFRKVPRNVPPEWKEYLGMPVSEVPDPWGCHDSYAEHFMEKFEREVKELGIE 124
I + DDYD RK+ +P E+++Y+GMP+SE+P P GC SYA+HF+ F +++LG+E
Sbjct: 60 IFIADDYDPLRKLYPFLPKEFEKYIGMPLSEIPCPQGCCKSYADHFLMPFLNSLEDLGVE 119
Query: 125 VEFLYASELYKRGEYSEEIRKAFEGKEKIMEILNKYREIAKQPPLPENWWPAMVYCPEHR 184
+ A+E YK G Y+E I A E + KI E+L+ YR + PL +NW+P V C +
Sbjct: 120 ITTHRANECYKAGMYNEAIITALENRLKIKELLDSYR----KEPLADNWYPLNVVCEKCG 175
Query: 185 K--EAEIIEWDGEWK-VKYRCPEGHEGWVDIRSGNVKLRWRVDWPMRWAHFDVDFEPAGK 241
K E ++ ++ E K + Y C G E V +G KL WRVDWP RW F V EP GK
Sbjct: 176 KMHETKVTSYNSEDKTITYVCKCGFENTVQPFNGIGKLPWRVDWPARWNIFGVTAEPMGK 235
Query: 242 DHLAAGSSYDTGKEIIKAIYGKEAPMSLMYEFVGIK-GEKG-KMSGSKGNVILLSDLYEV 299
DH A+G SYDTG +I + I+ +AP ++YE++ +K G+K MS S G V + D E+
Sbjct: 236 DHAASGGSYDTGIKIARQIFNYQAPEKMVYEWIQLKIGDKAMPMSSSSGVVFAVKDWTEI 295
Query: 300 LEPGLVRFIYARHRPNKEIRIDLGMGLLNLYDEFDKVERIYFGV------EDAKGDVEEL 353
P ++RF+ + +P K I DL + NL D++D++ER YF + E+ + E
Sbjct: 296 CHPEVLRFLILKGKPTKHIDFDL-KAISNLVDDYDELERKYFELIEKQKTEELNDNENEK 354
Query: 354 KRTYELSNPRIPNRLIAQAPFRFLAVLVQM------PHLDEDGIISVLTNQGHVPKELER 407
YEL P+IP RL Q +RF +++ Q+ +D + + +L G+ P E
Sbjct: 355 ISLYELVTPKIPERLPLQVAYRFCSIIAQIALDKETQKIDMERVFDILGRNGYNPAEFSE 414
Query: 408 EDIERIRLRIRLAKNWVEKYAPDSVKFSILPEPPKIEIDESVREAMEEVAKWIESKS--- 464
D R+ R+ ++K W Y + L + E EE W+++ S
Sbjct: 415 YDKSRLEKRLYMSKKWASDYGEN------LEINDFEQAKEQYETLSEEQKAWLKAFSKEV 468
Query: 465 ---TFSVDELNNVLFEVAKKRGISSKEWFSTLYKLFIGKERGPRLASFLASLDKDFVVRR 521
+ ++ +++E A K ++ KE F YK+ +GK GP+L SFLASL K+FV+ R
Sbjct: 469 ENIEIDANTIHELMYETATKLNLAPKEAFVASYKILLGKNYGPKLGSFLASLKKEFVIGR 528
Query: 522 LRL 524
L
Sbjct: 529 FNL 531
>gi|11498815 lysyl-tRNA synthetase (lysS) [Archaeoglobus fulgidus]
>gi|3183169|sp|O29052|SYK_ARCFU LYSYL-TRNA SYNTHETASE
(LYSINE--TRNA LIGASE) (LYSRS) >gi|7450807|pir||G69401
lysyl-tRNA synthetase (lysS) homolog - Archaeoglobus
fulgidus >gi|2649372|gb|AAB90029.1| (AE001020)
lysyl-tRNA synthetase (lysS) [Archaeoglobus fulgidus]
Length = 493
Score = 333 bits (845), Expect = 3e-90
Identities = 183/521 (35%), Positives = 296/521 (56%), Gaps = 33/521 (6%)
Query: 5 VHWADYVADKIIRERGEKDRYVVESGITPSGYVHVGNFRELFTAYIVGHALRDKGYEVRH 64
+HWAD +A +++ + + + +GI+PSG++H+GN RE+ TA + AL D G E +
Sbjct: 1 MHWADVIAADLLKR---SNSHRIATGISPSGHIHLGNLREMVTADAIRRALLDAGGEAKI 57
Query: 65 IHMWDDYDRFRKVPRNVPPEWKEYLGMPVSEVPDPWGCHDSYAEHFMEKFEREVKELGIE 124
+++ DD+D R+ +P E++ Y+GMP+ ++PDP GCHDSY+EHF++ F ++ LGI
Sbjct: 58 VYIADDFDPLRRRYPFLPEEYENYVGMPLCKIPDPEGCHDSYSEHFLQPFLESLEILGIP 117
Query: 125 VEFLYASELYKRGEYSEEIRKAFEGKEKIMEILNKYREIAKQPPLPENWWPAMVYCPE-- 182
VE A ++Y G Y R A + +++I I+ E+ + L E W+P M C
Sbjct: 118 VEVRRAYQMYSEGLYENNTRIALKRRDEIARII---AEVTGR-ELEERWYPFMPLCENCG 173
Query: 183 HRKEAEIIEWDGEWKVKYRCPEGHEGWVDIRSGNVKLRWRVDWPMRWAHFDVDFEPAGKD 242
+ +D W + Y C GH G V G KL WRVDW RW + EP GKD
Sbjct: 174 RINSTRVTSFDENW-IYYECDCGHSGRVGYVGGG-KLTWRVDWAARWQILSITCEPFGKD 231
Query: 243 HLAAGSSYDTGKEIIKAIYGKEAPMSLMYEFVGIKGEKGKMSGSKGNVILLSDLYEVLEP 302
H AAG SYDTG I + I+ E P + YE++ +KG KG M SKG V+ + ++ EV+ P
Sbjct: 232 HAAAGGSYDTGVRIAREIFDYEPPYPVPYEWIHLKG-KGAMKSSKGIVLPVREMVEVIPP 290
Query: 303 GLVRFIYARHRPNKEIRIDLGMGLLNLYDEFDKVERIYFGVEDAKGDVEELKRTYELSNP 362
+VR+I R +P + I D G+GLL+L +EF++ +E R+ ELS
Sbjct: 291 EIVRYITIRVKPERHIEFDPGLGLLDLVEEFEE-------------KFKEKDRSVELS-- 335
Query: 363 RIPNRLIAQAPFRFLAVLVQMPHLDEDGIISVLTNQGHVPKELEREDIERIRLRIRLAKN 422
+ + + PFR L V+ Q+ + D + + ++ G+ ++ R D+ER R++ A+
Sbjct: 336 LVGEVVYSDVPFRHLIVVGQIANWDLEKALEIIERTGYTVDDVTRRDVER---RLKYARK 392
Query: 423 WVEKYAPDSVKFSILPEPPKIEIDESVREAMEEVAKWIESKSTFSVDELNNVLFEVAKKR 482
W+EKYAPD++KF I PE E E ++ + A+ + +S +E++ ++++V+K+
Sbjct: 393 WLEKYAPDNIKFEI-PEKVTAEFSEEEKKFLRAYAERL--RSDMKPEEIHTLVYDVSKEV 449
Query: 483 GISSKEWFSTLYKLFIGKERGPRLASFLASLDKDFVVRRLR 523
GI S + F +YK +GK GPR+ F+ SL ++V R++
Sbjct: 450 GIKSSKAFQAIYKAILGKTYGPRVGYFIKSLGVEWVRERIK 490
>sp|O27585|SYK_METTH LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) (LYSRS)
>gi|7450806|pir||G69072 conserved hypothetical protein
MTH1542 - Methanobacterium thermoautotrophicum (strain
Delta H) >gi|2622662|gb|AAB86016.1| (AE000914) conserved
protein [Methanobacterium thermoautotrophicum]
Length = 544
Score = 332 bits (841), Expect = 8e-90
Identities = 186/537 (34%), Positives = 304/537 (55%), Gaps = 23/537 (4%)
Query: 1 MVIVVHWADYVADKIIRERGEKDRYVVESGITPSGYVHVGNFRELFTAYIVGHALRDKGY 60
++ + HW + +AD++ +ER + + +VV SG + SG +H+GN ++F A + +L+ G+
Sbjct: 18 VISLKHWIERIADEL-KER-DVEEHVVASGTSISGSIHIGNSCDVFIASSIAKSLKKDGF 75
Query: 61 EVRHIHMWDDYDRFRKVPRNVPPEWKEYLGMPVSEVPDPWGCHDSYAEHFMEKFEREVKE 120
+ R + + DD+D RKVP +P +++YLG+P S +P P GC +S+ EHF F ++
Sbjct: 76 KSRTVWIADDHDPLRKVPYPLPESYEKYLGVPYSMIPCPEGCCESFVEHFQRPFLEALER 135
Query: 121 LGIEVEFLYASELYKRGEYSEEIRKAFEGKEKIMEILNKYREIAKQPPLPENWWPAMVYC 180
I VE + +Y G Y++ IR + E +I EI N++R+ PL ++W P C
Sbjct: 136 FRIGVEHYSGARMYTEGLYNDYIRTSLERAPEIREIFNRFRD----RPLRDDWLPYNPIC 191
Query: 181 PE--HRKEAEIIEWDGEWKVKYRCPEGHEGWVDIRSGNVKLRWRVDWPMRWAHFDVDFEP 238
+ E ++ G+ V+YRC G +G +DI+SG KL WRV+W RW V EP
Sbjct: 192 EKCGRVNTTEAYDFSGD-TVRYRCECGFDGEMDIKSGLGKLTWRVEWAARWKILGVTCEP 250
Query: 239 AGKDHLAAGSSYDTGKEIIKAIYGKEAPMSLMYEFVGIKGEKGKMSGSKGNVILLSDLYE 298
GKDH A+G SYD I + I+ AP + YE++ ++GE MS S+G E
Sbjct: 251 FGKDHAASGGSYDVSSIISEEIFDYPAPYPVPYEWITLRGE--AMSKSRGVFFTPGQWLE 308
Query: 299 VLEPGLVRFIYARHRPNKEIRIDLGMGLLNLYDEFDKVERIYFGVEDAKGDVEE--LKRT 356
+ P + + R +P K + M L+L D+FD+ ERIY+G+EDA + EE L+
Sbjct: 309 IGPPESLNYFIFRSKPMKHKDFNPDMPFLDLMDQFDRTERIYYGMEDAASEKEEQKLRNI 368
Query: 357 YELSNPRIPNRLIAQAPFRFLAVLVQMPHLDEDGIISVLTNQGHVPKE--------LERE 408
Y +S L + +RF+ V Q+ D + + +L +P+E L +
Sbjct: 369 YRVSMIE-EFDLPLRPSYRFMTVACQIAGDDPERLYDILRRNSQLPEELMDLELDQLTDK 427
Query: 409 DIERIRLRIRLAKNWVEKYAPDSVKFSILPEPPKIEIDESVREAMEEVAKWIESKSTFSV 468
+E++ RI KNW+ YAP+ VKF + E P +E+ E + +++VA +ES+ S
Sbjct: 428 QLEQLNERIENVKNWLRLYAPEFVKFQVQEELPDVELSEPQLKFLQDVADLMESRE-MSA 486
Query: 469 DELNNVLFEVAKKRGISSKEWFSTLYKLFIGKERGPRLASFLASLDKDFVVRRLRLE 525
+EL++ ++ + ++ G+ ++ F +Y++ IGK+ GPR ASFL SL++DFV+RRLRLE
Sbjct: 487 EELHDEMYSILRRHGLKPQKAFQAIYRVLIGKKMGPRAASFLLSLERDFVIRRLRLE 543
>sp|P57677|SYK_THEAC LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) (LYSRS)
>gi|10640474|emb|CAC12288.1| (AL445066) lysyl-tRNA
synthetase (lysS) related protein [Thermoplasma
acidophilum]
Length = 507
Score = 298 bits (756), Expect = 8e-80
Identities = 177/514 (34%), Positives = 280/514 (54%), Gaps = 21/514 (4%)
Query: 12 ADKIIRERGEKDRYVVESGITPSGYVHVGNFRELFTAYIVGHALRDKGYEVRHIHMWDDY 71
AD ++++ R V +GI+PSG +HVGN RE+ T I+ A+ +G E I++ DD
Sbjct: 7 ADALVKDLSGPQR--VSTGISPSGPIHVGNMREILTGDILFKAITKRGLESDFIYLCDDM 64
Query: 72 DRFRKVPRNVPPEWKEYLGMPVSEVPDPWGCHDSYAEHFMEKFEREVKELGIEVEFLYAS 131
D RKV + +++ Y+G P+ +P P G Y+++++E F R +KE I + S
Sbjct: 65 DPLRKVYPFLSKDYERYVGQPLKNIPAPQGA-GKYSDYYLEPFVRVMKEANIPARVIKTS 123
Query: 132 ELYKRGEYSEEIRKAFEGKEKIMEILNKYREIAKQPPLPENWWPAMVYCPEHRK--EAEI 189
+LY+ G ++ A +EKI +IL E + +++P C + + +
Sbjct: 124 DLYESGMLAQACDIAINNREKIKDIL----ETVSGRKIEGDFYPYEPLCEKCGRISTTHV 179
Query: 190 IEWDGEWKVKYRCPEGHEGWVDIRSGNVKLRWRVDWPMRWAHFDVDFEPAGKDHLAAGSS 249
I + + +Y C GH G+ DIR K+ WRV+WP +W V EP GKDH A G S
Sbjct: 180 ISYSYPY-AEYACKCGHHGFADIRKAEGKMPWRVEWPAKWFALKVTVEPFGKDHGAPGGS 238
Query: 250 YDTGKEIIKAIYGKEAPMSLMYEFVGIKGEKGKMSGSKGNVILLSDLYEVLEPGLVRFIY 309
YDTG+ I + ++G E P+ LMYE + +KG KG M S G I S++ EV+ P L+R++
Sbjct: 239 YDTGRRIAREVFGIEPPVPLMYERIILKG-KGAMHSSTGLAIAASEIMEVIPPDLLRYMI 297
Query: 310 ARHRPNKEIRIDLGMGLLNLYDEFDKVERIYFGVEDAKGDVEELKRTYELSNPRIPNRLI 369
AR P + I D GMG+L L DE +K++ YF + + + Y L N +R
Sbjct: 298 ARVNPGRHIDFDPGMGILALSDELEKLQDAYFENRASLDEDQAAMVEYSLVN---KDRKP 354
Query: 370 AQAPFRFLAVLVQMPHLDEDGIISVLTNQGHVPKELEREDIERIRLRIRLAKNWVEKYAP 429
FR L LVQ+ +++ + +V Q P + D R I A+ W+E+YAP
Sbjct: 355 YPVDFRHLVTLVQIYRTEDEILRAVKKGQ---PSDFIEAD---FRKEIEYARRWLERYAP 408
Query: 430 DSVKFSILPEPPKIEIDESVREAMEEVAKWIESKSTFSVDELNNVLFEVAKKRGISSKEW 489
+SVKF ILP KIE+ +S + + IE ++ + +++ ++E+++K + +
Sbjct: 409 ESVKFRILPVDQKIELSDSDLAILSDFLNGIED-MPWNSESIHDRIYEISQKFKTNPESV 467
Query: 490 FSTLYKLFIGKERGPRLASFLASLDKDFVVRRLR 523
F+ Y++FIGK+RGPRL FL +L KDFV R+R
Sbjct: 468 FTLFYRVFIGKDRGPRLGYFLFNLGKDFVRERIR 501
>gb|AAG20183.1| (AE005095) lysyl-tRNA synthetase; LysS [Halobacterium sp. NRC-1]
Length = 548
Score = 267 bits (676), Expect = 2e-70
Identities = 170/550 (30%), Positives = 279/550 (49%), Gaps = 49/550 (8%)
Query: 7 WADYVADKIIRERGEKDRYVVESGITPSGYVHVGNFRELFTAYIVGHALRDKGYEVRHIH 66
WAD AD+++ + ++ V++ GI+PSG H+GN E+ Y V LR++G+EVR +
Sbjct: 18 WADAAADRVL-DGDPEEPIVIKGGISPSGVPHLGNMNEIMRGYFVAAVLRERGHEVRQVF 76
Query: 67 MWDDYDRFRKVPRNVPPEWKEY--------------LGMPVSEVPDPWGCHDSYAEHFME 112
DD D R +PR + E LG P +E+PDP+GC DSY HF
Sbjct: 77 TSDDRDPLRGLPRKLADLDGEVVALGDVNAGALGQNLGAPYTEIPDPFGCCDSYGAHFSN 136
Query: 113 KFEREVKELGIEVEFLYASELYKRGEYSEEIRKAFEGKEKIMEILNKYREIAKQPPLPEN 172
+ LG++VE + +E Y G + E R E ++L +Y++ + +P N
Sbjct: 137 LIQSSADRLGVDVEMVSNTETYANGGFEEVTRYLLEHVTAARDVLGEYQDKVDEEYVPFN 196
Query: 173 WWPAMVYCPEHRKEAEIIEWDGEWKVKYRCPE-----------GHEGWVDIRSGNVKLRW 221
P C + + ++ D V+Y C + GHEG +R G KL W
Sbjct: 197 --PICEACGKVTETVTDVDADA-GTVEYVCTDMDAGDQTIEGCGHEGTATLREG--KLPW 251
Query: 222 RVDWPMRWAHFDVDFEPAGKDHLAAGSSYDTGKEIIKAIYGKEAPMSLMYEFVGIKGEKG 281
R +WP +W VD+EP GKDH A S+ +G +I + + G E P ++YE+ + GE
Sbjct: 252 RFEWPAQWRTLGVDYEPFGKDH--AEGSWPSGTDISRNVLGDEPPTPMVYEWFTLDGE-- 307
Query: 282 KMSGSKGNVILLSDLYEVLEPGLVRFIYARH-RPNKEIRIDLGMGLLNLYDEFDKVERIY 340
S S G+VIL+ D+ +LE +VR+ +++ R ++ I+ L L DEFD++ER+Y
Sbjct: 308 PFSSSAGHVILVQDVLALLELPVVRYFFSKDPRRARDFSIE---HLDQLVDEFDRMERVY 364
Query: 341 FG--VEDAKGDVEELKRTYELSNPRIPNRLIAQAPFRFLAVLVQMPHLDEDGII--SVLT 396
FG + ++ + E R Y L I + + PF F AVL + ED + +
Sbjct: 365 FGEAADASEDERERAARVYPLLVDDIDADRV-RIPFTFAAVL----GMTEDSALREEIAR 419
Query: 397 NQGHVPKELEREDIERIRLRIRLAKNWVEKYAPDSVKFSILPEPPKIEIDESVREAMEEV 456
+GH+P + + + + R+ A+ W + + E P + D++ A++E+
Sbjct: 420 REGHIPADADADTVADALTRVERAREWARRTNNEFNYRLARAEIPAHDFDDATAAALDEL 479
Query: 457 AKWIESKSTFSVDELNNVLFEVAKKRGISSKEWFSTLYKLFIGKERGPRLASFLASLDKD 516
A +++ + + L ++E AK+ I +F+ Y+LF +++GP+L FLA LD++
Sbjct: 480 AAFLDDEDP-DGEALQGEIYEAAKRHDIDIGAFFAAGYRLFFDEDQGPKLGPFLAKLDRE 538
Query: 517 FVVRRLRLEG 526
FVV RLR EG
Sbjct: 539 FVVTRLRREG 548
>sp|Q9X895|SYK_STRCO LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) (LYSRS)
>gi|7480963|pir||T36123 probable lysyl-tRNA synthetase -
Streptomyces coelicolor (fragment)
>gi|4678917|emb|CAB41289.1| (AL049707) putative
lysyl-tRNA synthetase [Streptomyces coelicolor A3(2)]
Length = 545
Score = 252 bits (638), Expect = 5e-66
Identities = 170/546 (31%), Positives = 269/546 (49%), Gaps = 60/546 (10%)
Query: 26 VVESGITPSGYVHVGNFRELFTAYIVGHALRDKGYEVRHIHMWDDYDRFRKVPRNVP--- 82
VV SG++PSG +H+GN RE+ T ++V +R +G+EVRH+ WDDYDR+RKVP VP
Sbjct: 1 VVASGLSPSGPIHLGNLREVMTPHLVADEVRRRGHEVRHLISWDDYDRYRKVPAGVPGVD 60
Query: 83 PEWKEYLGMPVSEVPDPWGC-HDSYAEHFMEKFEREVKELGIEVEFLYASELYKRGEYSE 141
W E++G P++ VP P G H ++AEHF + E+G+E + + + Y G Y E
Sbjct: 61 ESWAEHIGKPLTSVPAPKGSPHPNWAEHFKAAMVDSLAEMGVEFDGISQTAQYTSGVYRE 120
Query: 142 EIRKAFEGKEKIMEILNKYR-----EIAKQPPLPE----------------------NWW 174
+I A + + I IL++YR Q PL E ++
Sbjct: 121 QILHAMKHRRDIDAILDQYRTKKAPAKKSQKPLDEAELEAAEGSGAAAEDDGSSGSAGYF 180
Query: 175 PAMVYCPEHRKEAEIIEW--DGEWKVKYRCPE-GHEGWVDIRSGN-VKLRWRVDWPMRWA 230
P YC K+ + D ++ Y C G V + N KL W+VDWPMRWA
Sbjct: 181 PYKPYCGNCEKDLTTVTAYDDDSTELTYACTACGFSETVRLSEFNRGKLVWKVDWPMRWA 240
Query: 231 HFDVDFEPAGKDHLAAGSSYDTGKEIIKAIYGKEAPMSLMYEFVGIKGEKGKMSGSKGNV 290
+ V FEP+G DH + GSS+ G +I+ I+G E P+ MY FVGI G KMS SKG V
Sbjct: 241 YEGVVFEPSGVDHSSPGSSFQVGGQIV-GIFGGEQPIGPMYAFVGISG-MAKMSSSKGGV 298
Query: 291 ILLSDLYEVLEPGLVRFIYARHRPNKEIRIDLGMGLLNLYDEFDKVERIYFGVEDAKGDV 350
+D +++EP L+R++YAR RPN+ +I + LYDE+D+++ D
Sbjct: 299 PTPADALKIMEPQLLRWLYARRRPNQSFKIAFDQEIQRLYDEWDRLDAKVADGSALPADA 358
Query: 351 EELKRTYELSNPRIPNRLIAQAPFRFLAVLVQMPHLDEDGIISVLTNQGHVPKELEREDI 410
R + +P R P+R LA + + ED + +L G + +
Sbjct: 359 AAHARAVGTAAGELP-RTPRPLPYRTLASVADITAGHEDQALRIL---GELDPANPIASL 414
Query: 411 ERIRLRIRLAKNWVEKYAPDSVKFSILPEPPKIEIDESVREAMEEVAKWIES--KSTFSV 468
+ R R A+ W+ + P + +I+ + P E+ +S+ E + + + +S+
Sbjct: 415 DEARPRYDKAEAWINTHVPADQR-TIVRQEPDAELLKSLDEPSRQSLRLLLDGLADHWSL 473
Query: 469 DELNNVLFEVAKKRG----------------ISSKEWFSTLYKLFIGKERGPRLASFLAS 512
D L + ++ V K + + + +F+ LY L +G++ GPRL + L +
Sbjct: 474 DGLTHHVYGVPKVQAGFPADATPKELPPEIKTAQRTFFALLYHLLVGRDTGPRLPTLLLA 533
Query: 513 LDKDFV 518
+ +D V
Sbjct: 534 VGQDRV 539
>sp|Q9YFT9|SYK_AERPE LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) (LYSRS)
>gi|7450809|pir||F72771 probable lysyl-tRNA Synthetase
APE0161 - Aeropyrum pernix (strain K1)
>gi|5103551|dbj|BAA79072.1| (AP000058) 562aa long
hypothetical lysyl-tRNA Synthetase [Aeropyrum pernix]
Length = 562
Score = 229 bits (577), Expect = 8e-59
Identities = 172/552 (31%), Positives = 271/552 (48%), Gaps = 54/552 (9%)
Query: 5 VHWADYVADKI---IRERGEKDRYVVESGITPSGYVHVGNFR-ELFTAYIVGHALRDKGY 60
VHW D + ++ ++ RG KD Y+ G++ SG H+G R E+ V L +G+
Sbjct: 20 VHWVDKLVAELEAKLQNRG-KDEYIFNGGLSVSGLQHIGRLRGEVLLGEAVRRELEKRGF 78
Query: 61 EVRH------IHMWDDYDRFRKVPRNVPPEWKEYLGMPVSEVPDPWGCHDSYAEHFMEKF 114
V+ + W D R+ + P + Y+G P+ VPDP GCH S+ +HF F
Sbjct: 79 RVKQLLTLYTVDPWKGKDEQRREFPD-PKAAERYVGWPLDRVPDPKGCHASWVDHFWSDF 137
Query: 115 EREVKELGI----EVEFLYASELYKRGEYSEEIRK-AFEGKEKIMEILNKYREIAKQPPL 169
+ G+ ++E + ELYK G E I +++I ++NKYR + P
Sbjct: 138 GPYI---GVFTDGKIEVVTTRELYK-GRLKEFITTMVLPRRDEIRRVINKYRG---RKPY 190
Query: 170 PENWWPAMVYCPEHRK--EAEIIEWDGEWKVKYRCPE-GHEGWVDIRSGNVKLRWRVDWP 226
E W P C + E +E G +V+YRC G++G I KL WR++W
Sbjct: 191 QEGWIPLEPRCARCGRIDSTEALEILGGERVRYRCSYCGYQGESSIEDS--KLNWRIEWA 248
Query: 227 MRWAHFDVDFEPAGKDHLAAGSSYDTGKEIIKAIYGKEAPMSLMYEFVGIK--GEKGKMS 284
W VDFEP GKDH G S D+ E+ + + G E P + YE+V ++ G + MS
Sbjct: 249 GVWWSLGVDFEPYGKDHATPGGSRDSAAELAR-LLGFEPPEGVWYEWVSLRAGGREADMS 307
Query: 285 GSKGNVILLSDLYEVLEPGLVRFIYARHRPNKEIRIDLGMGLLNLYDEFDKVERIYFGVE 344
S I + ++ P ++RFIY H P + + +DL + + Y ++ + ERIYFG+E
Sbjct: 308 SSGFTGITPREWLDIAHPQILRFIYFLHPPTRRVVVDLS-EIPSYYSQYYRAERIYFGIE 366
Query: 345 DAKGDVEE---LKRTYELSNP-RIPNRLIAQAPFRFLAVLVQM---PHLDEDGI--ISVL 395
+A VEE L RTYELS+P P + +Q P+ A++ Q+ L DG+ +
Sbjct: 367 EA-STVEETRYLARTYELSHPSNPPAKPPSQIPYSHAAIVAQVVGPERLWTDGLERLKRA 425
Query: 396 TNQGHVPKELEREDIERIRLRIRLAKNWVEKYAPDSVKFSILPEPPKIEIDESVR-EAME 454
GH + I + + A W +YAP +KF I PP+ + + + +E
Sbjct: 426 GLLGH-----DEYSIRWAKELLEKAYKWARRYAPKHLKFEIPDSPPEDALRRIEKPDLLE 480
Query: 455 EVAKWIESKSTFSVDELNNVLFEVAKKRGISSKE---WFSTLYKLFIGKERGPRLASFLA 511
++A+ +ES +S + + L E + G+SS E ++ Y +G+ GPR A L+
Sbjct: 481 KLAEVLESVEEWSEERIKQALVEFGE--GMSSSERRRFYRDFYLAIVGRPEGPRAAPLLS 538
Query: 512 SLDKDFVVRRLR 523
+D+ FVV RLR
Sbjct: 539 LMDRGFVVDRLR 550
>sp|O74059|SYK_CERSY LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) (LYSRS)
>gi|11280298|pir||T31311 lysine--tRNA ligase (EC
6.1.1.6) - Cenarchaeum symbiosum
>gi|3599397|gb|AAC62702.1| (AF083072) lysyl tRNA
synthetase [Cenarchaeum symbiosum]
Length = 524
Score = 186 bits (467), Expect = 6e-46
Identities = 155/540 (28%), Positives = 241/540 (43%), Gaps = 50/540 (9%)
Query: 7 WADYVADKII-RERG---EKDRYVVESGITPSGYVHVGNFRELFTAYIVGHALRDKGYEV 62
W D +A +++ RE + + VESG+ SG H+G+ + AY VG A+ D G+
Sbjct: 9 WIDKLAHELVEREEALGRDTEMINVESGLGASGIPHMGSLGDAVRAYGVGLAVGDMGHSF 68
Query: 63 RHIHMWDDYDRFRKVPRNVPPEWKEYLGMPVSEVPDPWGCHDSYAEHFMEKFEREVKELG 122
R I +DD D RKVP +P +E++ PVS +PDP+GCHDSY H + LG
Sbjct: 69 RLIAYFDDLDGLRKVPEGMPSSLEEHIARPVSAIPDPYGCHDSYGMHMSGLLLEGLDALG 128
Query: 123 IEVEFLYASELYKRGEYSEEIRKAFEGKEKIMEILNKYREIAKQPPLPENWWPAMVYCPE 182
IE +F A + Y+ G +E+I + I E K E+ Q + P C +
Sbjct: 129 IEYDFRRARDTYRDGLLAEQIHRILSNSSVIGE---KIAEMVGQEKFRSS-LPYFAVCEQ 184
Query: 183 HRK--EAEIIEWDGE-WKVKYRCPE-----------GHEGWVDIRSGNVKLRWRVDWPMR 228
K AE +E+ + KV+YRC + GHEG D KL W+V++ R
Sbjct: 185 CGKMYTAESVEYLADSRKVRYRCGDAEVGGRKIAGCGHEGEADTGGAGGKLAWKVEFAAR 244
Query: 229 WAHFDVDFEPAGKDHLAAGSSYDTGKEIIKAIYGKEAPMSLMYEFVGIKGEKGKMSGSKG 288
W FDV FE GKD + S + I P YE KG K K+S S G
Sbjct: 245 WQAFDVRFEAYGKDIM---DSVRINDWVSDEILSSPHPHHTRYEMFLDKGGK-KISKSSG 300
Query: 289 NVILLSDLYEVLEP-GLVRFIYARHRPNKEIRIDLGMGLLNLYDEFDKVERIYFGVEDAK 347
NV+ P ++ +Y R +E+ ++ + +L DE+ ++R YF
Sbjct: 301 NVVTPQKWLRYGTPQSILLLMYKRITGARELGLE---DVPSLMDEYGDLQREYFAGGGRG 357
Query: 348 GDVEELKRTYELSNPRIPNRLIAQAPFRFLAVLVQMPHLDEDG----IISVLTNQGHVPK 403
G E K + P +LV++ L + + L G +
Sbjct: 358 GKAREAKNRGLFEYTNLLEAQEGPRPHAGYRLLVELSRLFRENRTERVTKKLVEYGVI-- 415
Query: 404 ELEREDIERIRLRIRLAKNWVEKYAPDSVKFSILPEPPKIEIDESVREAMEEVAKWIESK 463
+ IER+ I LA N YA D E ++E+D + R A+ E+A+ + S
Sbjct: 416 DGPSPGIERL---IALAGN----YADDMYS----AERTEVELDGATRGALSELAEMLGSA 464
Query: 464 STFSVDELNNVLFEVAKKRGISSKEWFSTLYKLFIGKERGPRLASFLASLDKDFVVRRLR 523
L +V++ VAK G+ +++F LY++ + GPR+ F+ + ++ V +R
Sbjct: 465 PE---GGLQDVIYGVAKSHGVPPRDFFKALYRIILDASSGPRIGPFIEDIGREKVAGMIR 521
>sp|Q9ZDF8|SYK_RICPR LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) (LYSRS)
>gi|7467883|pir||D71694 lysine--tRNA ligase (EC 6.1.1.6)
(lysS) RP371 - Rickettsia prowazekii
>gi|3860930|emb|CAA14830.1| (AJ235271) LYSYL-tRNA
SYNTHETASE (lysS) [Rickettsia prowazekii]
Length = 528
Score = 150 bits (376), Expect = 3e-35
Identities = 134/513 (26%), Positives = 228/513 (44%), Gaps = 35/513 (6%)
Query: 8 ADYVADKIIRERGEKDRYVVESGITPSGYVHVGNFRELFTAYIVGHALRD-KGYEVRHIH 66
A + D + + EK + E+G PSG H+G F E +V A + I
Sbjct: 19 AKKILDSLNGKAPEKGYILFETGYGPSGLPHIGTFAENARMVMVQKAFEQLSDIPTKLIC 78
Query: 67 MWDDYDRFRKVPRNVP-PEW-KEYLGMPVSEVPDPWGCHDSYAEHFMEKFEREVKELGIE 124
DD D RKVP N+P PE +Y+ MP++ +PDP+G SY + K + + G +
Sbjct: 79 FSDDMDGLRKVPSNIPHPEMVAQYMDMPLTSIPDPFGKCKSYGHYMNAKLCAFLDKFGFK 138
Query: 125 VEFLYASELYKRGEYSEEIRKAFEGKEKIMEI-LNKYREIAKQPPLPENWWPAMVYCPEH 183
EF ++ YK G + E + + E ++IM + L +R+ K + P M CP+
Sbjct: 139 YEFYSSTNCYKAGMFDEMLIRVLEKYDEIMALMLPTFRDERKT-----TYAPFMPICPKT 193
Query: 184 RK--EAEIIEWDGE-WKVKYRCPEGHEGWVDIRSGNVKLRWRVDWPMRWAHFDVDFEPAG 240
K + I +WD + V Y+ +G++ V + G+ KL+W+ D+ MRWA VD+E G
Sbjct: 194 GKVLQVPIEKWDAKAGTVSYKDEDGNDVEVPVTGGHCKLQWKPDFGMRWAALKVDYEMYG 253
Query: 241 KDHLAAGSSYDTGKEIIKAIYGKEAPMSLMYE-FVGIKGEKGKMSGSKGNVILLSD-LYE 298
KDHLA Y EI + + GK P+ YE F+ GE K+S S+GN I + D L
Sbjct: 254 KDHLANSRLY---SEICRILGGK-PPVQFCYELFLDANGE--KISKSRGNSISVDDWLKY 307
Query: 299 VLEPGLVRFIYARHRPNKEIRIDLGMGLLNLYDEFDKVERIYFGVEDAKGDVEELKRTYE 358
+ F+Y K + DL + DE+ + + Y ED Y
Sbjct: 308 ASVESIALFMYKNPARAKRLFFDL---IPKNVDEYITLNQKYHLEEDMV--TRFANPVYH 362
Query: 359 LSNPRIPNRLIAQAPFRFLAVLVQMPHLDEDGIISVLTNQGHVPKELEREDIERIRLRIR 418
+ + +P + L L + + + ++ + + PK L
Sbjct: 363 IHHGNVPKIETFGLTYSLLLNLTAVCNTSDKSVLWGFITK-YEPKATPNTSTYLDHL-TE 420
Query: 419 LAKNWVEKYAPDSVKFSILPEPPKIEIDESVREAMEEVAKWIESKSTFSVDELNNVLFEV 478
A + + + ++ E KI I + + + ++ E +S + ++++
Sbjct: 421 FAIRYYNDFIQTHKSYLVVSEKHKI-ILHDILDMLSNISDQTEEES------IQKAIYDI 473
Query: 479 AKKRGISS-KEWFSTLYKLFIGKERGPRLASFL 510
K G + + +F LY++ +G+ GPRL +F+
Sbjct: 474 GMKSGYENLRYYFKDLYQILLGQNEGPRLGTFI 506
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.320 0.140 0.431
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 212412843
Number of Sequences: 2977
Number of extensions: 9889628
Number of successful extensions: 24858
Number of sequences better than 1.0e-10: 15
Number of HSP's better than 0.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 24762
Number of HSP's gapped (non-prelim): 15
length of query: 526
length of database: 189,106,746
effective HSP length: 53
effective length of query: 473
effective length of database: 157,386,935
effective search space: 74444020255
effective search space used: 74444020255
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 167 (69.5 bits)