BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0145 (asnA-2) DE:PLANT- TYPE L-ASPARAGINASE (EC 3.5.1.1)
(L-ASPARAGINE AMIDOHYDROLASE)
(305 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||A75211 asparaginase (EC 3.5.1.1) PAB0145 [similarity] - Pyr... 606 e-172
sp|O57971|ASGX_PYRHO PUTATIVE L-ASPARAGINASE (L-ASPARAGINE AMIDO... 565 e-160
pir||G82055 probable asparaginase VC2603 [imported] - Vibrio cho... 197 1e-49
gb|AAF48471.1| (AE003499) CG7860 gene product [Drosophila melano... 176 3e-43
sp|P37595|ASGX_ECOLI PUTATIVE L-ASPARAGINASE PRECURSOR (L-ASPARA... 175 6e-43
sp|P30364|ASPG_LUPAN L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE... 168 6e-41
sp|Q9ZSD6|ASPG_LUPLU L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE... 168 1e-40
sp|P30362|ASPG_LUPAR L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE... 167 1e-40
sp|P50288|ASPG_LUPAL L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE... 167 2e-40
pir||T50495 asparaginase - Arabidopsis thaliana >gi|8346547|emb|... 166 2e-40
gb|AAG20072.1| (AE005086) Vng1872c [Halobacterium sp. NRC-1] 164 1e-39
sp|P50287|ASPG_ARATH L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE... 163 2e-39
dbj|BAB02681.1| (AB012247) l-asparaginase (l-asparagine amidohyd... 161 1e-38
emb|CAC11482.1| (AL445064) L-asparaginase related protein [Therm... 160 2e-38
sp|P74383|ASGX_SYNY3 PUTATIVE L-ASPARAGINASE (L-ASPARAGINE AMIDO... 158 7e-38
gb|AAK42501.1| Asparinase, putative (ansA-like) [Sulfolobus sol... 155 7e-37
pdb|9GAF|A Chain A, Precursor Of The W11f Mutant Glycosylasparag... 146 3e-34
sp|Q47898|ASPG_FLAME N4-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINA... 146 3e-34
emb|CAC09349.1| (AL442007) putative l-asparaginase [Oryza sativa] 145 6e-34
pdb|9GAA|A Chain A, Precursor Of The T152a Mutant Glycosylaspara... 144 1e-33
pdb|9GAC|A Chain A, Precursor Of The T152c Mutant Glycosylaspara... 144 1e-33
sp|O02467|ASPG_SPOFR N4-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINA... 143 3e-33
gb|AAG28786.1|AF308474_1 (AF308474) asparaginase [Hordeum vulgare] 141 7e-33
gb|AAA35903.1| (M64073) glycosylasparaginase [Homo sapiens] 132 6e-30
gi|4557273 aspartylglucosaminidase precursor [Homo sapiens] >gi|... 131 8e-30
gb|AAB60655.1| (U21281) lysosomal glycosylasparaginase [Homo sap... 131 8e-30
sp|Q64191|ASPG_MOUSE N4-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINA... 130 2e-29
gi|11446109 aspartylglucosaminidase precursor [Homo sapiens] 130 2e-29
dbj|BAB15302.1| (AK025969) unnamed protein product [Homo sapiens] 125 5e-28
gi|8923202 hypothetical protein FLJ20212 [Homo sapiens] >gi|7020... 121 9e-27
dbj|BAB15209.1| (AK025671) unnamed protein product [Homo sapiens] 121 9e-27
gi|11421701 hypothetical protein FLJ20212 [Homo sapiens] 121 9e-27
gb|AAF58918.1| (AE003832) CG1827 gene product [Drosophila melano... 120 2e-26
dbj|BAB08998.1| (AB012239) glycosylasparaginase-like protein [Ar... 107 2e-22
sp|Q21697|ASPG_CAEEL PUTATIVE N4-(BETA-N-ACETYLGLUCOSAMINYL)-L-A... 104 1e-21
pdb|1AYY|C Chain C, Glycosylasparaginase >gi|3212474|pdb|1AYY|A ... 98 1e-19
pir||T01223 hypothetical protein F6N23.5 - Arabidopsis thaliana ... 91 1e-17
emb|CAB90154.1| (AL355013) similar to L-asparaginase [Schizosacc... 87 3e-16
gb|AAF49544.1| (AE003528) CG5241 gene product [Drosophila melano... 86 6e-16
pir||T49437 asparaginase related protein [imported] - Neurospora... 80 3e-14
pdb|1APY|A Chain A, Human Aspartylglucosaminidase >gi|1942817|pd... 79 7e-14
pdb|1APY|B Chain B, Human Aspartylglucosaminidase >gi|1942818|pd... 73 3e-12
>pir||A75211 asparaginase (EC 3.5.1.1) PAB0145 [similarity] - Pyrococcus abyssi
(strain Orsay) >gi|5457645|emb|CAB49136.1| (AJ248283)
PLANT-TYPE L-ASPARAGINASE (EC 3.5.1.1) (L-ASPARAGINE
AMIDOHYDROLASE) [Pyrococcus abyssi]
Length = 305
Score = 606 bits (1545), Expect = e-172
Identities = 305/305 (100%), Positives = 305/305 (100%)
Query: 1 MVAIIVHGGAGTIRKEERIPKVLEGVREAVLAGWKELKKGSALDAVEEAIKVLEDNPIFN 60
MVAIIVHGGAGTIRKEERIPKVLEGVREAVLAGWKELKKGSALDAVEEAIKVLEDNPIFN
Sbjct: 1 MVAIIVHGGAGTIRKEERIPKVLEGVREAVLAGWKELKKGSALDAVEEAIKVLEDNPIFN 60
Query: 61 AGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGA 120
AGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGA
Sbjct: 61 AGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGA 120
Query: 121 VKFARIMGFPEYDPTTEERRKQWQELKEKLMKGEVRHWKKLGELIKEHPEVLRSTVGAVA 180
VKFARIMGFPEYDPTTEERRKQWQELKEKLMKGEVRHWKKLGELIKEHPEVLRSTVGAVA
Sbjct: 121 VKFARIMGFPEYDPTTEERRKQWQELKEKLMKGEVRHWKKLGELIKEHPEVLRSTVGAVA 180
Query: 181 FDGEEVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAIKLALAKTAT 240
FDGEEVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAIKLALAKTAT
Sbjct: 181 FDGEEVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAIKLALAKTAT 240
Query: 241 DFVRLGLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKNTKHMSYAFMKEGMNE 300
DFVRLGLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKNTKHMSYAFMKEGMNE
Sbjct: 241 DFVRLGLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKNTKHMSYAFMKEGMNE 300
Query: 301 PEAGV 305
PEAGV
Sbjct: 301 PEAGV 305
>sp|O57971|ASGX_PYRHO PUTATIVE L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE)
>gi|7449813|pir||A71247 probable L-asparaginase -
Pyrococcus horikoshii >gi|3256621|dbj|BAA29304.1|
(AP000001) 305aa long hypothetical L-asparaginase
[Pyrococcus horikoshii]
Length = 305
Score = 565 bits (1440), Expect = e-160
Identities = 278/305 (91%), Positives = 298/305 (97%)
Query: 1 MVAIIVHGGAGTIRKEERIPKVLEGVREAVLAGWKELKKGSALDAVEEAIKVLEDNPIFN 60
MVAIIVHGGAGTI+KEERIPKV+EGV+EAV+ GWKEL+KGSALDAVEEAIKVLEDNPIFN
Sbjct: 1 MVAIIVHGGAGTIKKEERIPKVIEGVKEAVIVGWKELRKGSALDAVEEAIKVLEDNPIFN 60
Query: 61 AGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGA 120
AGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGA
Sbjct: 61 AGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGA 120
Query: 121 VKFARIMGFPEYDPTTEERRKQWQELKEKLMKGEVRHWKKLGELIKEHPEVLRSTVGAVA 180
VKFAR+MGFPEY+P TEER +QW+ELKEKLMKGE+++WKKLGELIKE+PEVLRSTVGAVA
Sbjct: 121 VKFARLMGFPEYNPITEERIEQWKELKEKLMKGEIKYWKKLGELIKEYPEVLRSTVGAVA 180
Query: 181 FDGEEVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAIKLALAKTAT 240
FDGEE+VAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAI+LALAKTAT
Sbjct: 181 FDGEEIVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAIRLALAKTAT 240
Query: 241 DFVRLGLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKNTKHMSYAFMKEGMNE 300
DFVRLG+DAQAAS AAI LATK+FGKDTMGIIMVD+ GNVGFAKNTKHMSYA+MK+GM E
Sbjct: 241 DFVRLGMDAQAASNAAISLATKYFGKDTMGIIMVDAAGNVGFAKNTKHMSYAYMKDGMEE 300
Query: 301 PEAGV 305
PEAGV
Sbjct: 301 PEAGV 305
>pir||G82055 probable asparaginase VC2603 [imported] - Vibrio cholerae (group
O1 strain N16961) >gi|9657191|gb|AAF95744.1| (AE004327)
asparaginase, putative [Vibrio cholerae]
Length = 326
Score = 197 bits (496), Expect = 1e-49
Identities = 131/303 (43%), Positives = 183/303 (60%), Gaps = 17/303 (5%)
Query: 3 AIIVHGGAGTIRKEERIPK----VLEGVREAVLAGWKELKKGS-ALDAVEEAIKVLEDNP 57
AI +HGGAGTI +E+ +L + +AV AG + L +G+ ALDAV A+ VLED+P
Sbjct: 6 AIAIHGGAGTILREQMSDALRMDILATLEQAVRAGHQLLVEGADALDAVVAAVTVLEDSP 65
Query: 58 IFNAGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVG 117
+FNAG GSVLT + VEMDA++M G EAGA+AG+ ++NPI +AR V+ +DHV L+G
Sbjct: 66 LFNAGKGSVLTHNEMVEMDASVMHGAAREAGAIAGVRHIRNPIQLARDVLRHSDHVFLIG 125
Query: 118 EGAVKFARIMG--FPEYDP-TTEERRKQWQELKEK----LMKGEVRHWKKLGELIKEHPE 170
+GA +FA G + E D TE R +Q Q +K++ L + + E E+P+
Sbjct: 126 DGAEQFAFQQGHVYTEQDYFFTERRYEQLQNMKQQDRFALSEASYQSESAEQESPSEYPD 185
Query: 171 VLR-STVGAVAFDGE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYA-NEVAGASCTGLG 227
+ TVGAVA D + + A TSTGG+ K FGRVGD+PIIGAGT A N SCTG+G
Sbjct: 186 DKKFGTVGAVALDQQGNLAAATSTGGITNKRFGRVGDSPIIGAGTLAENGNVAISCTGMG 245
Query: 228 EVAIKLALAKTATDFVR-LGLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKNT 286
E I+ A+A +R L D A E ++ K G + G+I +D++G + FA N+
Sbjct: 246 EYFIRYAVAGDIAARMRYLKEDVHTACETVVQGELKSVGGEG-GLIAIDAQGELHFAMNS 304
Query: 287 KHM 289
M
Sbjct: 305 SGM 307
>gb|AAF48471.1| (AE003499) CG7860 gene product [Drosophila melanogaster]
Length = 332
Score = 176 bits (441), Expect = 3e-43
Identities = 114/310 (36%), Positives = 176/310 (56%), Gaps = 19/310 (6%)
Query: 4 IIVHGGAGTIRKEERIPKVLEGVREAVLAGWKELKK------GSALDAVEEAIKVLEDNP 57
+++HGGAG I + RI G+++A+ + W L GSALDAVE A++ +E +
Sbjct: 6 LLIHGGAGDI-SDSRIAGKFAGIKQALRSAWGLLSPDNGSGGGSALDAVEAAVRSMELDE 64
Query: 58 IFNAGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVG 117
FNAG GS L G+VE++A++M G+ L AG + + V +PI+VAR++MEK H L G
Sbjct: 65 NFNAGYGSCLNTSGQVELEASLMEGRDLRAGCITLLRDVMHPITVARRLMEKQRHTFLGG 124
Query: 118 EGAVKFARIMGFPEYDP---TTEERRKQWQELKEKLMKGEVRHWKKLGELIKEHP----E 170
A + A G P TE R +E ++++ +G+ + + EL + P +
Sbjct: 125 AAAQELALATGSERLQPGALVTEGARLTLKEFEDQVAQGKDPFFART-ELTDDKPVPKTD 183
Query: 171 VLRSTVGAVAFDGE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEV-AGASCTGLGE 228
TVGAVA D ++V GTSTGG+ K GR+GDTPI+G+GTYA+ G S TG GE
Sbjct: 184 PSGETVGAVAMDASGQIVVGTSTGGITGKWPGRIGDTPILGSGTYADNCRGGVSTTGHGE 243
Query: 229 VAIKLALAKTATDFVRL-GLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKNTK 287
++ LA+ + GL AQAA++ TK G T G I+V G++G + ++
Sbjct: 244 TLMRYNLAQRILSAMEYQGLSAQAAADKECREMTKRLG-GTGGAIVVGHSGDLGISFTSR 302
Query: 288 HMSYAFMKEG 297
M++ ++++G
Sbjct: 303 RMAWGYVQDG 312
>sp|P37595|ASGX_ECOLI PUTATIVE L-ASPARAGINASE PRECURSOR (L-ASPARAGINE AMIDOHYDROLASE)
>gi|7449811|pir||D64820 probable asparaginase (EC
3.5.1.1) ybiK - Escherichia coli
>gi|1787050|gb|AAC73915.1| (AE000185) putative
asparaginase [Escherichia coli K12]
>gi|4062400|dbj|BAA35516.1| (D90720) Hypothetical
protein in moeA-grxA intergenic region precursor .
[Escherichia coli] >gi|4062405|dbj|BAA35523.1| (D90721)
Hypothetical protein in moeA-grxA intergenic region
precursor . [Escherichia coli]
Length = 321
Score = 175 bits (439), Expect = 6e-43
Identities = 122/319 (38%), Positives = 173/319 (53%), Gaps = 31/319 (9%)
Query: 4 IIVHGGAGTIRKEE----RIPKVLEGVREAVLAGWKELKKG-SALDAVEEAIKVLEDNPI 58
I +HGGAG I + + + + +E + V G K L+ G SALD V EA+++LE+ P+
Sbjct: 6 IAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLEAGESALDVVTEAVRLLEECPL 65
Query: 59 FNAGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGE 118
FNAG G+V T D E+DA +M G TL+AGAVAG+ ++NP+ AR VME++ HV+++GE
Sbjct: 66 FNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSHLRNPVLAARLVMEQSPHVMMIGE 125
Query: 119 GAVKFARIMGFPEYDPTTEERRKQWQELKEKLMKGEVRHWKKLGELIKEH------PEVL 172
GA FA G P ++++L +K G + +H +
Sbjct: 126 GAENFAFARGMERVSPEIFSTSLRYEQLLAA---------RKEGATVLDHSGAPLDEKQK 176
Query: 173 RSTVGAVAFDGE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGA-SCTGLGEVA 230
TVGAVA D + + A TSTGG+ K+ GRVGD+P++GAG YAN + A SCTG GEV
Sbjct: 177 MGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVF 236
Query: 231 IKLALAKTATDFVRL----GLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKNT 286
I+ A A D L GL A E + G + G+I +D GNV NT
Sbjct: 237 IR---ALAAYDIAALMDYGGLSLAEACERVVMEKLPALG-GSGGLIAIDHEGNVALPFNT 292
Query: 287 KHMSYAFMKEGMNEPEAGV 305
+ M A+ G + P G+
Sbjct: 293 EGMYRAWGYAG-DTPTTGI 310
>sp|P30364|ASPG_LUPAN L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE) >gi|99970|pir||S24757
asparaginase (EC 3.5.1.1) - narrow-leaved blue lupine
>gi|19135|emb|CAA43099.1| (X60691) developing seed
L-asparaginase [Lupinus angustifolius]
Length = 325
Score = 168 bits (422), Expect = 6e-41
Identities = 119/313 (38%), Positives = 165/313 (52%), Gaps = 23/313 (7%)
Query: 3 AIIVHGGAGTIR----KEERIPKVLEGVREAVLAGWKELKKGSA-LDAVEEAIKVLEDNP 57
+I +HGGAG I E R P+ EG+R + G + LK LD VE ++ LE+
Sbjct: 5 SIALHGGAGDIPFSLPPERRQPRE-EGLRHCLQIGVEALKSQKPPLDVVELVVRELENIQ 63
Query: 58 IFNAGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVG 117
FNAG GSVLT G VEM+A+IM GKT++ GAV+G+ V NPIS+AR VM+KT H+ L
Sbjct: 64 HFNAGIGSVLTNSGTVEMEASIMDGKTMKCGAVSGLSTVLNPISLARLVMDKTPHIYLAF 123
Query: 118 EGAVKFARIMGFPEYDPTTEERRKQWQELKEKLMKGEVR-HWKKLG-----------ELI 165
+GA FA+ G D + + + LK + V+ + + EL
Sbjct: 124 QGAQDFAKQQGVETVDSSHFITAENVERLKLAIEANRVQVDYSQYNYPQPAQDDAEKELP 183
Query: 166 KEHPEVLRSTVGAVAFDGE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCT 224
+ + TVG VA D + + TSTGG+ KM GR+GDTP+IGAGTYANE+ S T
Sbjct: 184 LANGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSAT 243
Query: 225 GLGEVAIKLALAKTATDFVRL-GLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFA 283
G GE I +A+ + GL + A++ + T K T+G+I V + G +
Sbjct: 244 GKGEAIISATVARDVAALMEFKGLSLKEAADYVVHERTP---KGTVGLIAVSAAGEIAMP 300
Query: 284 KNTKHMSYAFMKE 296
NT M A E
Sbjct: 301 FNTTGMFRASATE 313
>sp|Q9ZSD6|ASPG_LUPLU L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE)
>gi|4139266|gb|AAD03742.1| (AF112444) L-asparaginase
[Lupinus luteus]
Length = 325
Score = 168 bits (420), Expect = 1e-40
Identities = 120/313 (38%), Positives = 165/313 (52%), Gaps = 23/313 (7%)
Query: 3 AIIVHGGAGTIR----KEERIPKVLEGVREAVLAGWKELK-KGSALDAVEEAIKVLEDNP 57
+I +HGGAG I E R P+ EG+R + G + LK + LD VE ++ LE+
Sbjct: 5 SIALHGGAGDIPFSLPPERRKPRE-EGLRHCLQIGVEALKAQKPPLDVVELVVRELENIE 63
Query: 58 IFNAGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVG 117
FNAG GSVLT G VEM+A+IM G T++ GAV+G+ V NPIS+AR VM+KT H+ L
Sbjct: 64 HFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLSTVLNPISLARLVMDKTPHIYLAF 123
Query: 118 EGAVKFARIMGFPEYDPTTEERRKQWQELKEKLMKGEVR---HWKKLGELIKEHPE---- 170
+GA FA+ G D + + + LK + V+ E +K+ E
Sbjct: 124 QGAQDFAKQQGVETVDSSHLITAENVERLKLAIEANRVQVDYSQYNYPEPVKDDAEKELP 183
Query: 171 -----VLRSTVGAVAFDGE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCT 224
TVG VA D + + TSTGG+ KM GR+GDTP+IGAGTYANE+ S T
Sbjct: 184 LTNGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSAT 243
Query: 225 GLGEVAIKLALAKTATDFVRL-GLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFA 283
G GE I+ +A+ + GL + A++ I T K T+G+I V + G +
Sbjct: 244 GKGEEIIRATVARDVAALMEFKGLSLKEAADFVIHERTP---KGTVGLIAVSAAGEIAMP 300
Query: 284 KNTKHMSYAFMKE 296
NT M A E
Sbjct: 301 FNTTGMFRACATE 313
>sp|P30362|ASPG_LUPAR L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE) >gi|81837|pir||S22523
asparaginase (EC 3.5.1.1) [validated] - tree lupine
(fragment) >gi|19137|emb|CAA36824.1| (X52588)
asparaginase [Lupinus arboreus]
Length = 306
Score = 167 bits (419), Expect = 1e-40
Identities = 115/299 (38%), Positives = 162/299 (53%), Gaps = 19/299 (6%)
Query: 16 EERIPKVLEGVREAVLAGWKELK-KGSALDAVEEAIKVLEDNPIFNAGTGSVLTIDGKVE 74
E R P+ EG+R + G + LK + S LD VE ++ LE+N FNAG GSVLT G VE
Sbjct: 3 ERRKPRE-EGLRHCLQIGVEALKARKSPLDVVELVVRELENNEHFNAGIGSVLTNSGTVE 61
Query: 75 MDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFPEYDP 134
M+A+IM GK+++ GAV+G+ V NPIS+AR VMEKT H+ L +GA FA+ G D
Sbjct: 62 MEASIMDGKSMKCGAVSGLSTVLNPISLARLVMEKTPHMYLAFQGAQDFAKQQGVETVDS 121
Query: 135 TTEERRKQWQELKEKLMKGEVR-HWKKLG-----------ELIKEHPEVLRSTVGAVAFD 182
+ + + LK + V+ + + EL + + TVG VA D
Sbjct: 122 SHFITAENVERLKLAIEANRVQIDYSQYNYTQPVQDDAEKELPVANGDSQIGTVGCVAVD 181
Query: 183 GE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAIKLALAKTATD 241
+ + + TSTGG+ KM GR+GDTP+IGAGTYANE+ S TG GE I+ +A+
Sbjct: 182 SQGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSATGKGEAIIQATVARDVAA 241
Query: 242 FVRL-GLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKNTKHMSYAFMKEGMN 299
+ GL + A++ + T K T+G+I V + G + NT M A E N
Sbjct: 242 LMEFKGLSLKEAADYVVHERTP---KGTVGLIAVSAAGEIAMPFNTTGMFRACATEDGN 297
>sp|P50288|ASPG_LUPAL L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE)
>gi|496102|gb|AAA33409.1| (L19141) L-asparaginase
[Lupinus albus]
Length = 325
Score = 167 bits (418), Expect = 2e-40
Identities = 116/313 (37%), Positives = 165/313 (52%), Gaps = 23/313 (7%)
Query: 3 AIIVHGGAGTIR----KEERIPKVLEGVREAVLAGWKELK-KGSALDAVEEAIKVLEDNP 57
+I +HGGAG I E R P+ EG+R + G + LK + LD VE ++ LE+
Sbjct: 5 SIALHGGAGDIPFSLPPERRKPRE-EGLRHCLQIGIEALKAQNPPLDVVELVVRELENIQ 63
Query: 58 IFNAGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVG 117
FNAG GSVLT G VEM+A+IM G T++ GAV+G+ V NPIS+AR+VM+KT H+ L
Sbjct: 64 HFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLNTVMNPISLARQVMDKTPHIFLAF 123
Query: 118 EGAVKFARIMGFPEYDPTTEERRKQWQELKEKLMKGEVR-HWKKLG-----------ELI 165
+GA + G D + + + LK + V+ + + EL
Sbjct: 124 QGAQDLGKQQGVETVDSSHFITEENVERLKLAIEANRVQVDYSQYNYTQPVQDDAEKELP 183
Query: 166 KEHPEVLRSTVGAVAFDGE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCT 224
+ + TVG VA D + + TSTGG+ KM GR+GDTP+IGAGTYANE+ S T
Sbjct: 184 LANGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSAT 243
Query: 225 GLGEVAIKLALAKTATDFVRL-GLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFA 283
G GE I+ +A+ + GL + A++ + T K T+G+I V + G +
Sbjct: 244 GKGEAIIRATVARDVAALMEFKGLSLKEAADCVVHERTP---KGTVGLIAVSAAGEIAMP 300
Query: 284 KNTKHMSYAFMKE 296
NT M A E
Sbjct: 301 FNTTGMFRACATE 313
>pir||T50495 asparaginase - Arabidopsis thaliana >gi|8346547|emb|CAB93711.1|
(AL357612) asparaginase [Arabidopsis thaliana]
Length = 315
Score = 166 bits (417), Expect = 2e-40
Identities = 116/303 (38%), Positives = 160/303 (52%), Gaps = 13/303 (4%)
Query: 3 AIIVHGGAGTIR----KEERIPKVLEGVREAVLAGWKELKKGSA-LDAVEEAIKVLEDNP 57
AI +HGGAG I E RIP+ +R + G LK G LD E ++ LE++P
Sbjct: 5 AIALHGGAGDIPIDLPDERRIPRE-SALRHCLDLGISALKSGKPPLDVAELVVRELENHP 63
Query: 58 IFNAGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVG 117
FNAG GSVLT G VEM+A+IM GKT GAV+G+ V NPIS+AR VMEKT H+ L
Sbjct: 64 DFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAF 123
Query: 118 EGAVKFARIMGFPEYDPT---TEERRKQWQELKEKLMKGEVRHWKKLGELIKEHPEVLRS 174
+ A FAR G D + T E + ++ KE + ++ + ++ +
Sbjct: 124 DAAEAFARAHGVETVDSSHFITPENIARLKQAKE-FNRVQLDYTVPSPKVPDNCGDSQIG 182
Query: 175 TVGAVAFDGE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAIKL 233
TVG VA D + + TSTGG KM GR+GDTP+IGAGTYAN + S TG GE I+
Sbjct: 183 TVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKGEDIIRG 242
Query: 234 ALAKTATDFVRLGLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKNTKHMSYAF 293
+A+ + + +EAA + + + + G++ V + G V NT M A
Sbjct: 243 TVARDVAALME--YKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGMFRAC 300
Query: 294 MKE 296
E
Sbjct: 301 ASE 303
>gb|AAG20072.1| (AE005086) Vng1872c [Halobacterium sp. NRC-1]
Length = 294
Score = 164 bits (411), Expect = 1e-39
Identities = 117/299 (39%), Positives = 150/299 (50%), Gaps = 19/299 (6%)
Query: 4 IIVHGGAGTIRKEERIPKVLEGVREAVL--AGWKELKKGSALDAVEEAIKVLEDNPIFNA 61
++VHGGAG P R+ L A + +DAV A++VLE +P FNA
Sbjct: 3 VLVHGGAGA-------PPDAPAPRQDTLETAADTGASHATPVDAVTAAVRVLERDPAFNA 55
Query: 62 GTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAV 121
GTG+ DG + DA +M AGA A + GV+ ++VAR VM T HVLL G AV
Sbjct: 56 GTGATPQSDGVIRTDAGMMTSDRA-AGAAASMPGVEAAVAVARAVMADTPHVLLSGVHAV 114
Query: 122 KFARIMGFP-EYDPTTEERRKQWQELKEKLMKGEVRHWKKLGELI-------KEHPEVLR 173
FA G P E D T + R +W +L H + E +E P
Sbjct: 115 DFAADTGIPVESDLWTADTRARWDDLDSHPDGSPTDHRDWVRERFGRTDPGGRESPPGDH 174
Query: 174 STVGAVAFDGEEVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAIKL 233
TVGAVA DG+ TSTGG +L + GRVGD P IGAG YA AS TG GE K
Sbjct: 175 DTVGAVASDGDRFATATSTGGRWLALAGRVGDVPQIGAGFYAAPAGAASATGAGEAIAKT 234
Query: 234 ALAKTATDFVRLGLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKNTKHMSYA 292
L++ A + GL AQAA++ AIE ++ G + G+I+VD G G A N M A
Sbjct: 235 TLSRRAVRHLEAGLGAQAAADRAIEEFSELTGA-SAGVIVVDRDGGCGTAHNAAAMQTA 292
>sp|P50287|ASPG_ARATH L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE)
>gi|1076292|pir||S53127 asparaginase (EC 3.5.1.1) -
Arabidopsis thaliana >gi|735918|emb|CAA84367.1| (Z34884)
asparaginase [Arabidopsis thaliana]
Length = 315
Score = 163 bits (409), Expect = 2e-39
Identities = 118/306 (38%), Positives = 155/306 (50%), Gaps = 19/306 (6%)
Query: 3 AIIVHGGAGTIR----KEERIPKVLEGVREAVLAGWKELKKGSA-LDAVEEAIKVLEDNP 57
AI +HGGAG I E RIP+ +R + G LK G LD E ++ LE++P
Sbjct: 5 AIALHGGAGDIPIDLPDERRIPRE-SALRHCLDLGISALKSGKPPLDVAELVVRELENHP 63
Query: 58 IFNAGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVG 117
FNAG GSVLT G VEM+A+IM GKT GAV+G+ V NPIS+AR VMEKT H+ L
Sbjct: 64 DFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAF 123
Query: 118 EGAVKFARIMGFPE------YDPTTEERRKQWQELKEKLMKGEVRHWKKLGELIKEHPEV 171
+ A FAR G P R KQ +E + V K + +
Sbjct: 124 DAAEAFARAHGVETVYSSHFITPENIARLKQAKEFNRVQLDYTVPSPK----VPDNCGDS 179
Query: 172 LRSTVGAVAFDGE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVA 230
TVG VA D + + TSTGG KM GR+GDTP+IGAGTYAN + S TG GE
Sbjct: 180 QIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKGEDI 239
Query: 231 IKLALAKTATDFVRLGLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKNTKHMS 290
I+ +A+ + + +EAA + + + + G++ V + G V NT M
Sbjct: 240 IRGTVARDVAALME--YKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGMF 297
Query: 291 YAFMKE 296
A E
Sbjct: 298 RACASE 303
>dbj|BAB02681.1| (AB012247) l-asparaginase (l-asparagine amidohydrolase)
[Arabidopsis thaliana]
Length = 325
Score = 161 bits (403), Expect = 1e-38
Identities = 120/306 (39%), Positives = 158/306 (51%), Gaps = 27/306 (8%)
Query: 3 AIIVHGGAGTIRK--EERIPKVLEGVREAVLAGWKELKKG-SALDAVEEAIKVLEDNPIF 59
AI VHGGAG ER + + + + G L+ SA+D VE I+ LE +P+F
Sbjct: 5 AIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELETDPLF 64
Query: 60 NAGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEG 119
N+G GS LT G VEM+A+IM G GAV+GI VKNPIS+AR VM+K+ H L G
Sbjct: 65 NSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYLAFSG 124
Query: 120 AVKFARIMGFP----EYDPTTEE--RRKQWQELKEKLMKGEVRHWKKLGELIKEHPEVLR 173
A FAR G EY T + K +E L + G + P +
Sbjct: 125 AEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSPIQMN 184
Query: 174 ---------STVGAVAFDGE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASC 223
TVG V DG+ AGTSTGG+ KM GR+GD+P+IGAGTYA+E G SC
Sbjct: 185 GLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFCGVSC 244
Query: 224 TGLGEVAIKLALAKTATDFVRL-GLDAQAASEAAIELATKH-FGKDTMGIIMVDSRGNV- 280
TG GE I+ LA+ + + GL+ Q A++ KH + G+I V ++G V
Sbjct: 245 TGEGEAIIRATLARDVSAVMEYKGLNLQ----EAVDYVIKHRLDEGFAGLIAVSNKGEVV 300
Query: 281 -GFAKN 285
GF N
Sbjct: 301 CGFNSN 306
>emb|CAC11482.1| (AL445064) L-asparaginase related protein [Thermoplasma
acidophilum]
Length = 274
Score = 160 bits (400), Expect = 2e-38
Identities = 102/234 (43%), Positives = 135/234 (57%), Gaps = 17/234 (7%)
Query: 4 IIVHGGAGTIRKEERIPKVLEGVREAVLAGWKELKKGSALDAVEEAIKVLEDNPIFNAGT 63
+++HGG G+ + L G E +EL SALD A+ +ED+P FNAGT
Sbjct: 5 LLIHGGVGS---DPSFNGRLNGYAETAS---EEL---SALDNAVRAVIKMEDDPDFNAGT 55
Query: 64 GSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVKF 123
GSV IDG ++MDAA+M G+V I VKNPI VAR VM K+ H+++ G+GAVKF
Sbjct: 56 GSVPRIDGSIQMDAAVMIPGHF--GSVINIERVKNPILVARDVMAKSPHIMMTGDGAVKF 113
Query: 124 ARIMGFPEYDPTTEERRKQWQELKEKLMKGEV-RHWKKLGELIKEHPEVLRSTVGAVAFD 182
AR+MG+P+YDP TE+ R ++ +E + K EV +K + ++ H TVGAVA
Sbjct: 114 ARLMGYPDYDPMTEKARNLLKKFEEDVEKNEVPDKFKIYFKYVRVH-----DTVGAVARV 168
Query: 183 GEEVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAIKLALA 236
E STGG F M GRVGD+PI GAG Y E TG+GE K L+
Sbjct: 169 EGEFAGAVSTGGSFPMMRGRVGDSPIPGAGIYVGERGAVVATGVGEEIAKNVLS 222
>sp|P74383|ASGX_SYNY3 PUTATIVE L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE)
>gi|7469484|pir||S76221 hypothetical protein -
Synechocystis sp. (strain PCC 6803)
>gi|1653567|dbj|BAA18480.1| (D90914) asparaginase
[Synechocystis sp.]
Length = 329
Score = 158 bits (396), Expect = 7e-38
Identities = 106/287 (36%), Positives = 160/287 (54%), Gaps = 17/287 (5%)
Query: 4 IIVHGGAGTIRKEERIPKVLEGVREAVLAGWKELKKG-SALDAVEEAIKVLEDNPIFNAG 62
+I+HGGA ++ + + V + + + V A ++ L G SA+DAV + ++LE+ P FNAG
Sbjct: 5 LIIHGGASSLDDKGGLATVRQSLHQIVAAVYETLTAGGSAMDAVVQGCELLENEPRFNAG 64
Query: 63 TGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVK 122
TGSVL DG+V M A++M G V + +KNPI +A+ + +TD + L GA
Sbjct: 65 TGSVLQSDGQVRMSASLMDGDRQNFSGVINVSRIKNPIQMAQFLQGQTDRI-LSDYGAAD 123
Query: 123 FARIMGFPEYDPTTEERRKQWQELKEKLMKGEVRHWKKLGELIKEHP---EVLRSTVGAV 179
AR M P YDP T+ R ++W E + + +VR KK+ LI + E + T+G V
Sbjct: 124 LAREMQLPIYDPATDFRIQEWMEERGE----DVR--KKMARLIADPTVGIEARKGTIGVV 177
Query: 180 AFDGE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAIKLALAKT 238
A D ++ AGTSTGG L+ GRV D+ + AG YA AG SCTG+GE I LA
Sbjct: 178 ALDANGKIAAGTSTGGKGLERIGRVSDS-AMPAGNYATRFAGVSCTGVGEDIINECLAAK 236
Query: 239 ATDFVRLGLDAQAASEAAIELATKHFGKDT-MGIIMVDSRGNVGFAK 284
V+ D Q ++A + T+ +T +G I +D +G++ + K
Sbjct: 237 VVIRVK---DGQNLAQAMAKSITEALENNTDLGAIALDHQGHIAWGK 280
>gb|AAK42501.1| Asparinase, putative (ansA-like) [Sulfolobus solfataricus]
Length = 276
Score = 155 bits (387), Expect = 7e-37
Identities = 102/288 (35%), Positives = 149/288 (51%), Gaps = 39/288 (13%)
Query: 4 IIVHGGAGTIRKEERIPKVLEGVREAVLAGWKELKKGSALDAVEEAIKVLEDNPIFNAGT 63
++VHGGAG+ + ++ K + EA+ G+ E +KGSA++AV EAI +E++ +F+AG
Sbjct: 8 LVVHGGAGSWQIADQ-DKAKLTISEALERGYYEFRKGSAIEAVVEAIYYMEESGVFDAGK 66
Query: 64 GSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVKF 123
GSV G +EMDA IM G TL+ G + G+ + I A +++ + HVL++G
Sbjct: 67 GSVRNSAGYIEMDAGIMVGNTLQVGGIMGL-REGSAIKKALEILFQNRHVLMIGSN---- 121
Query: 124 ARIMGFPEYDPTTEERRKQWQELKEKLMKGEVRHWKKLGELIKEHPEVLRSTVGAVAFDG 183
Y+ + ++ +V TVGAVA D
Sbjct: 122 --------YNSSNSNNSAIYES------------------------KVSGDTVGAVALDQ 149
Query: 184 E-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAIKLALAKTATDF 242
+VAGTSTGG+ K+ GRVGD+PI GAG YA S TG+GE+ +++ AK
Sbjct: 150 HGNLVAGTSTGGIKGKLPGRVGDSPIPGAGYYATSNVAVSSTGIGEIILRILPAKEVDIL 209
Query: 243 VRLGLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKNTKHMS 290
V LG A A + TK FGKD +G+I +D GN NTK M+
Sbjct: 210 VSLGFTIDDALRAVVNKVTKTFGKDNIGMIGLDKYGNASAYYNTKGMA 257
>pdb|9GAF|A Chain A, Precursor Of The W11f Mutant Glycosylasparaginase From
Flavobacterium Meningosepticum >gi|9257088|pdb|9GAF|C
Chain C, Precursor Of The W11f Mutant
Glycosylasparaginase From Flavobacterium Meningosepticum
Length = 295
Score = 146 bits (365), Expect = 3e-34
Identities = 102/265 (38%), Positives = 146/265 (54%), Gaps = 26/265 (9%)
Query: 29 AVLAGWKELKKGS-ALDAVEEAIKVLEDNPIFNA-GTGSVLTIDGKVEMDAAIMRGKTLE 86
A + WK L KG ALDAVE+ ++++ED+P + G G DG+V +DA IM +
Sbjct: 17 ANVEAWKVLSKGGKALDAVEKGVRLVEDDPTERSVGYGGRPDRDGRVTLDACIM-DENYN 75
Query: 87 AGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFPEYDPTTEERRKQWQE- 145
G+VA + +KNPISVAR VMEKT HV+LVG+GA++FA GF + + T E K+W+E
Sbjct: 76 IGSVACMEHIKNPISVARAVMEKTPHVMLVGDGALEFALSQGFKKENLLTAESEKEWKEW 135
Query: 146 LKEKLMKGEVRHWKKLGELIKEHPEVLRSTVGAVAFDGEEVVAGT-STGGVFLKMFGRVG 204
LK K V I+ H T+G +A D + ++G +T G+ KM GRVG
Sbjct: 136 LKTSQYKPIVN--------IENH-----DTIGMIALDAQGNLSGACTTSGMAYKMHGRVG 182
Query: 205 DTPIIGAGTYA-NEVAGASCTGLGEVAIKLALAKTATDFVRLGLDAQAASEAAIELATK- 262
D+PIIGAG + NE+ A+ TG GE I+ + + G Q A + A+E K
Sbjct: 183 DSPIIGAGLFVDNEIGAATATGHGEEVIRTVGTHLVVELMNQGRTPQQACKEAVERIVKI 242
Query: 263 --HFGKD----TMGIIMVDSRGNVG 281
GK+ +G I ++ +G G
Sbjct: 243 VNRRGKNLKDIQVGFIALNKKGEYG 267
>sp|Q47898|ASPG_FLAME N4-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASE PRECURSOR
(GLYCOSYLASPARAGINASE) (ASPARTYLGLUCOSAMINIDASE)
(N4-(N-ACETYL-BETA-GLUCOSAMINYL)-L-ASPARAGINE AMIDASE)
(AGA) >gi|2120584|pir||S69194
N4-(beta-N-acetylglucosaminyl)-L-asparaginase (EC
3.5.1.26) precursor [validated] - Flavobacterium
meningosepticum >gi|555668|gb|AAA68868.1| (U08028)
glycosylasparaginase precursor [Chryseobacterium
meningosepticum]
Length = 340
Score = 146 bits (365), Expect = 3e-34
Identities = 102/265 (38%), Positives = 146/265 (54%), Gaps = 26/265 (9%)
Query: 29 AVLAGWKELKKGS-ALDAVEEAIKVLEDNPIFNA-GTGSVLTIDGKVEMDAAIMRGKTLE 86
A + WK L KG ALDAVE+ ++++ED+P + G G DG+V +DA IM +
Sbjct: 62 ANVEAWKVLSKGGKALDAVEKGVRLVEDDPTERSVGYGGRPDRDGRVTLDACIM-DENYN 120
Query: 87 AGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFPEYDPTTEERRKQWQE- 145
G+VA + +KNPISVAR VMEKT HV+LVG+GA++FA GF + + T E K+W+E
Sbjct: 121 IGSVACMEHIKNPISVARAVMEKTPHVMLVGDGALEFALSQGFKKENLLTAESEKEWKEW 180
Query: 146 LKEKLMKGEVRHWKKLGELIKEHPEVLRSTVGAVAFDGEEVVAGT-STGGVFLKMFGRVG 204
LK K V I+ H T+G +A D + ++G +T G+ KM GRVG
Sbjct: 181 LKTSQYKPIVN--------IENH-----DTIGMIALDAQGNLSGACTTSGMAYKMHGRVG 227
Query: 205 DTPIIGAGTYA-NEVAGASCTGLGEVAIKLALAKTATDFVRLGLDAQAASEAAIELATK- 262
D+PIIGAG + NE+ A+ TG GE I+ + + G Q A + A+E K
Sbjct: 228 DSPIIGAGLFVDNEIGAATATGHGEEVIRTVGTHLVVELMNQGRTPQQACKEAVERIVKI 287
Query: 263 --HFGKD----TMGIIMVDSRGNVG 281
GK+ +G I ++ +G G
Sbjct: 288 VNRRGKNLKDIQVGFIALNKKGEYG 312
>emb|CAC09349.1| (AL442007) putative l-asparaginase [Oryza sativa]
Length = 333
Score = 145 bits (362), Expect = 6e-34
Identities = 110/320 (34%), Positives = 158/320 (49%), Gaps = 41/320 (12%)
Query: 3 AIIVHGGAGTIRKEERIPKVLEGVREAVLA-----GWKELKKGSA-LDAVEEAIKVLEDN 56
AI +HGGAG + +P+ + + VLA G L+ G+A LD VE ++ LE +
Sbjct: 5 AIAIHGGAGV---DPNLPEHRQEEAKRVLARCLQVGVDALRSGAAALDVVEAVVRELESD 61
Query: 57 PIFNAGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLV 116
P FN+G GS LT G VEM+A+IM G+ GAV+G+ VKNP+S+AR VM+K+ H L
Sbjct: 62 PFFNSGRGSALTRLGTVEMEASIMDGRGRRCGAVSGVSTVKNPVSLARLVMDKSPHSYLA 121
Query: 117 GEGAVKFARIMGFPEYDPT---TEERRKQWQELKE--------------------KLMKG 153
+GA +FAR G D + TE+ + KE + +
Sbjct: 122 FDGAEQFARDQGLEVVDNSYFITEDNVGMLKLAKEANSILFDYRIPLSGSETCGAQAVSS 181
Query: 154 EVRHWKKLGELIKEHPEVLRS--TVGAVAFDGE-EVVAGTSTGGVFLKMFGRVGDTPIIG 210
+ H G ++ P + + TVG D A TSTGG+ KM GR+GD+P+IG
Sbjct: 182 DNSHGN--GMVMNGLPISIYAPETVGCAVVDSSGHTAAATSTGGLMNKMTGRIGDSPLIG 239
Query: 211 AGTYANEVAGASCTGLGEVAIKLALAKTATDFVRL-GLDAQAASEAAIELATKHFGKDTM 269
+GTYA SCTG GE I+ LA+ + G Q A + ++ + +
Sbjct: 240 SGTYACGACAVSCTGEGEAIIRSTLARDVAAVMEYRGAGLQEAVDYCVK---ERLDEGFA 296
Query: 270 GIIMVDSRGNVGFAKNTKHM 289
G+I V S G V + N M
Sbjct: 297 GLIAVSSTGEVAYGFNCTGM 316
>pdb|9GAA|A Chain A, Precursor Of The T152a Mutant Glycosylasparaginase From
Flavobacterium Meningosepticum >gi|9257084|pdb|9GAA|C
Chain C, Precursor Of The T152a Mutant
Glycosylasparaginase From Flavobacterium Meningosepticum
Length = 295
Score = 144 bits (360), Expect = 1e-33
Identities = 101/265 (38%), Positives = 145/265 (54%), Gaps = 26/265 (9%)
Query: 29 AVLAGWKELKKGS-ALDAVEEAIKVLEDNPIFNA-GTGSVLTIDGKVEMDAAIMRGKTLE 86
A + WK L KG ALDAVE+ ++++ED+P + G G DG+V +DA IM +
Sbjct: 17 ANVEAWKVLSKGGKALDAVEKGVRLVEDDPTERSVGYGGRPDRDGRVTLDACIM-DENYN 75
Query: 87 AGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFPEYDPTTEERRKQWQE- 145
G+VA + +KNPISVAR VMEKT HV+LVG+GA++FA GF + + T E K+W+E
Sbjct: 76 IGSVACMEHIKNPISVARAVMEKTPHVMLVGDGALEFALSQGFKKENLLTAESEKEWKEW 135
Query: 146 LKEKLMKGEVRHWKKLGELIKEHPEVLRSTVGAVAFDGEEVVAGT-STGGVFLKMFGRVG 204
LK K V I+ H +G +A D + ++G +T G+ KM GRVG
Sbjct: 136 LKTSQYKPIVN--------IENH-----DAIGMIALDAQGNLSGACTTSGMAYKMHGRVG 182
Query: 205 DTPIIGAGTYA-NEVAGASCTGLGEVAIKLALAKTATDFVRLGLDAQAASEAAIELATK- 262
D+PIIGAG + NE+ A+ TG GE I+ + + G Q A + A+E K
Sbjct: 183 DSPIIGAGLFVDNEIGAATATGHGEEVIRTVGTHLVVELMNQGRTPQQACKEAVERIVKI 242
Query: 263 --HFGKD----TMGIIMVDSRGNVG 281
GK+ +G I ++ +G G
Sbjct: 243 VNRRGKNLKDIQVGFIALNKKGEYG 267
>pdb|9GAC|A Chain A, Precursor Of The T152c Mutant Glycosylasparaginase From
Flavobacterium Meningosepticum >gi|9257086|pdb|9GAC|C
Chain C, Precursor Of The T152c Mutant
Glycosylasparaginase From Flavobacterium Meningosepticum
Length = 295
Score = 144 bits (359), Expect = 1e-33
Identities = 101/265 (38%), Positives = 145/265 (54%), Gaps = 26/265 (9%)
Query: 29 AVLAGWKELKKGS-ALDAVEEAIKVLEDNPIFNA-GTGSVLTIDGKVEMDAAIMRGKTLE 86
A + WK L KG ALDAVE+ ++++ED+P + G G DG+V +DA IM +
Sbjct: 17 ANVEAWKVLSKGGKALDAVEKGVRLVEDDPTERSVGYGGRPDRDGRVTLDACIM-DENYN 75
Query: 87 AGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFPEYDPTTEERRKQWQE- 145
G+VA + +KNPISVAR VMEKT HV+LVG+GA++FA GF + + T E K+W+E
Sbjct: 76 IGSVACMEHIKNPISVARAVMEKTPHVMLVGDGALEFALSQGFKKENLLTAESEKEWKEW 135
Query: 146 LKEKLMKGEVRHWKKLGELIKEHPEVLRSTVGAVAFDGEEVVAGT-STGGVFLKMFGRVG 204
LK K V I+ H +G +A D + ++G +T G+ KM GRVG
Sbjct: 136 LKTSQYKPIVN--------IENH-----DCIGMIALDAQGNLSGACTTSGMAYKMHGRVG 182
Query: 205 DTPIIGAGTYA-NEVAGASCTGLGEVAIKLALAKTATDFVRLGLDAQAASEAAIELATK- 262
D+PIIGAG + NE+ A+ TG GE I+ + + G Q A + A+E K
Sbjct: 183 DSPIIGAGLFVDNEIGAATATGHGEEVIRTVGTHLVVELMNQGRTPQQACKEAVERIVKI 242
Query: 263 --HFGKD----TMGIIMVDSRGNVG 281
GK+ +G I ++ +G G
Sbjct: 243 VNRRGKNLKDIQVGFIALNKKGEYG 267
>sp|O02467|ASPG_SPOFR N4-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASE
(GLYCOSYLASPARAGINASE) (ASPARTYLGLUCOSAMINIDASE)
(N4-(N-ACETYL-BETA-GLUCOSAMINYL)-L-ASPARAGINE AMIDASE)
(AGA) >gi|7322070|gb|AAB50885.2| (S83425)
glycosylasparaginase alpha beta [Spodoptera frugiperda]
Length = 320
Score = 143 bits (356), Expect = 3e-33
Identities = 105/276 (38%), Positives = 142/276 (51%), Gaps = 24/276 (8%)
Query: 34 WKELK-KGSALDAVEEAIKVLEDNPIFNA-GTGSVLTIDGKVEMDAAIMRGKTLEAGAVA 91
W+ LK +G ALDAVE+ V E+ G G DG+ +DA IM G T+ GAVA
Sbjct: 21 WEVLKDEGKALDAVEQGGIVCENEQCDRTVGYGGSPDEDGETTLDAFIMDGSTMNVGAVA 80
Query: 92 GIWGVKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFPEYDPTTEERRKQWQELK---- 147
+ +K+ ISVAR VME T H L GE A KFA MGF E +T+E R+ W + +
Sbjct: 81 ALRRIKSAISVARHVMEYTTHSFLAGELATKFAVEMGFKEESLSTDESRELWSKWRFEKQ 140
Query: 148 -----EKLMKGEVR-----HWKKLGELIKEHPEVL------RSTVGAVAFDGE-EVVAGT 190
K +K + R + KK + HPEV T+G VA D + +V AGT
Sbjct: 141 CQPNFRKNVKPDPRKHCGPYHKKRNFVDYIHPEVFVVDQYNHDTIGMVAVDSKGDVAAGT 200
Query: 191 STGGVFLKMFGRVGDTPIIGAGTYA-NEVAGASCTGLGEVAIKLALAKTATDFVRLGLDA 249
ST G K+ GRVGD+PI GAG YA N V GA+ TG G+ ++ + A + +R G
Sbjct: 201 STNGAKFKIPGRVGDSPIPGAGAYADNTVGGAAATGNGDTMMRFLPSFLAVEEMRRGASP 260
Query: 250 QAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKN 285
A++ AI+ + H +I + G G A N
Sbjct: 261 ANAAKTAIKRISAHHPDFMGAVIALSKNGQYGAACN 296
>gb|AAG28786.1|AF308474_1 (AF308474) asparaginase [Hordeum vulgare]
Length = 333
Score = 141 bits (353), Expect = 7e-33
Identities = 109/318 (34%), Positives = 153/318 (47%), Gaps = 37/318 (11%)
Query: 3 AIIVHGGAGTIRKEERIPKVLEGVREAVLA-----GWKELKKGS-ALDAVEEAIKVLEDN 56
AI +HGGAG + +P+ + + VLA G L+ G+ ALD VE ++ LE +
Sbjct: 5 AIAIHGGAGV---DPNLPEHRQEEAKRVLARCLQVGVDLLRAGATALDVVEAVVRELETD 61
Query: 57 PIFNAGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLV 116
P FN+G GS LT G VEM+A+IM G+ GAV+G+ VKNP+S+AR+VM+++ H L
Sbjct: 62 PCFNSGRGSALTRAGTVEMEASIMDGRGRRCGAVSGVSTVKNPVSLARRVMDRSPHSYLA 121
Query: 117 GEGAVKFARIMGFPEYDPT---TEERRKQWQELKE------------------KLMKGEV 155
+GA FAR G D + TEE + KE
Sbjct: 122 FDGAEDFAREQGLEVVDNSYFITEENVGMLKLAKEANSILFDYRIPLAGADTCSAQAAAT 181
Query: 156 RHWKKLGELIKEHPEVLRS--TVGAVAFD-GEEVVAGTSTGGVFLKMFGRVGDTPIIGAG 212
+ G ++ P + + TVG D A TSTGG+ KM GR+GD+P+IGAG
Sbjct: 182 ENHNNNGMVMNGLPISIYAPETVGCAVVDCNGFTAAATSTGGLMNKMTGRIGDSPLIGAG 241
Query: 213 TYANEVAGASCTGLGEVAIKLALAKTATDFVRLGLDAQAASEAAIELATK-HFGKDTMGI 271
TYA SCTG GE I+ LA+ D + + A++ K + G+
Sbjct: 242 TYACGHCAVSCTGEGEAIIRSTLAR---DVAAVMESRGCLLQEAVDFCVKERLDEGFAGL 298
Query: 272 IMVDSRGNVGFAKNTKHM 289
I V G V + N M
Sbjct: 299 IAVSGTGEVAYGFNCTGM 316
>gb|AAA35903.1| (M64073) glycosylasparaginase [Homo sapiens]
Length = 346
Score = 132 bits (328), Expect = 6e-30
Identities = 97/287 (33%), Positives = 139/287 (47%), Gaps = 33/287 (11%)
Query: 27 REAVLAGWKELKKG-SALDAVEEAIKVLE----DNPIFNAGTGSVLTIDGKVEMDAAIMR 81
+ A A W+ L G SALDAVE + E D + G+ L G+ +DA IM
Sbjct: 37 KNATEAAWRALASGGSALDAVESGCAMCEREQCDGSVGFGGSPDEL---GETTLDAMIMD 93
Query: 82 GKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFPEYDPTTEERRK 141
G T++ GAV + +KN I VARKV+E T H LLVGE A FA+ MGF D +T +
Sbjct: 94 GTTMDVGAVGDLRRIKNAIGVARKVLEHTTHTLLVGESATTFAQSMGFINEDLSTSASQA 153
Query: 142 QWQELKEKLMKGEVRHWKK-----------------LGELIKEHPEVL----RSTVGAVA 180
+ + + +W+ L + I H E T+G V
Sbjct: 154 LHSDWLAQ--NSQPNYWRNVIPDPSKYCGPYKPPGILKQDIPIHKETEDDRGHDTIGMVV 211
Query: 181 FDGE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAG-ASCTGLGEVAIKLALAKT 238
+ AGTST G+ K+ GRVGD+PI GAG YA++ AG A+ TG G++ ++ +
Sbjct: 212 IHKTGHIAAGTSTNGIKFKIHGRVGDSPIPGAGAYADDTAGAAAATGNGDILMRFLPSYQ 271
Query: 239 ATDFVRLGLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKN 285
A +++R G D A + I KHF + +I + G+ G A N
Sbjct: 272 AVEYMRRGEDPTIACQKVISRIQKHFPEFFGAVICANVTGSYGAACN 318
>gi|4557273 aspartylglucosaminidase precursor [Homo sapiens]
>gi|114276|sp|P20933|ASPG_HUMAN
N4-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASE PRECURSOR
(GLYCOSYLASPARAGINASE) (ASPARTYLGLUCOSAMINIDASE)
(N4-(N-ACETYL-BETA-GLUCOSAMINYL)-L-ASPARAGINE AMIDASE)
(AGA) >gi|67759|pir||MUHUGD
N4-(beta-N-acetylglucosaminyl)-L-asparaginase (EC
3.5.1.26) precursor [validated] - human
>gi|28534|emb|CAA39029.1| (X55330)
N4-(beta-N-acetylglucosaminyl)-L- asparaginase [Homo
sapiens] >gi|34760|emb|CAA39288.1| (X55762)
glycosylasparaginase precursor (AA -23 to 323) [Homo
sapiens]
Length = 346
Score = 131 bits (327), Expect = 8e-30
Identities = 99/285 (34%), Positives = 139/285 (48%), Gaps = 29/285 (10%)
Query: 27 REAVLAGWKELKKG-SALDAVEEAIKVLE----DNPIFNAGTGSVLTIDGKVEMDAAIMR 81
+ A A W+ L G SALDAVE + E D + G+ L G+ +DA IM
Sbjct: 37 KNATEAAWRALASGGSALDAVESGCAMCEREQCDGSVGFGGSPDEL---GETTLDAMIMD 93
Query: 82 GKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFPEYDPTTEERR- 140
G T++ GAV + +KN I VARKV+E T H LLVGE A FA+ MGF D +T +
Sbjct: 94 GTTMDVGAVGDLRRIKNAIGVARKVLEHTTHTLLVGESATTFAQSMGFINEDLSTSASQA 153
Query: 141 ------------KQWQELKEKLMK--GEVRHWKKLGELIKEHPEVL----RSTVGAVAFD 182
W+ + K G + L + I H E T+G V
Sbjct: 154 LHSDWLARNCQPNYWRNVIPDPSKYCGPYKPPGILKQDIPIHKETEDDRGHDTIGMVVIH 213
Query: 183 GE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAG-ASCTGLGEVAIKLALAKTAT 240
+ AGTST G+ K+ GRVGD+PI GAG YA++ AG A+ TG G++ ++ + A
Sbjct: 214 KTGHIAAGTSTNGIKFKIHGRVGDSPIPGAGAYADDTAGAAAATGNGDILMRFLPSYQAV 273
Query: 241 DFVRLGLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKN 285
+++R G D A + I KHF + +I + G+ G A N
Sbjct: 274 EYMRRGEDPTIACQKVISRIQKHFPEFFGAVICANVTGSYGAACN 318
>gb|AAB60655.1| (U21281) lysosomal glycosylasparaginase [Homo sapiens]
Length = 346
Score = 131 bits (327), Expect = 8e-30
Identities = 99/285 (34%), Positives = 139/285 (48%), Gaps = 29/285 (10%)
Query: 27 REAVLAGWKELKKG-SALDAVEEAIKVLE----DNPIFNAGTGSVLTIDGKVEMDAAIMR 81
+ A A W+ L G SALDAVE + E D + G+ L G+ +DA IM
Sbjct: 37 KNATEAAWRALASGGSALDAVESGCAMCEREQCDGSVGFGGSPDEL---GETTLDAMIMD 93
Query: 82 GKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFPEYDPTTEERR- 140
G T++ GAV + +KN I VARKV+E T H LLVGE A FA+ MGF D +T +
Sbjct: 94 GTTMDVGAVGDLRRIKNAIGVARKVLEHTTHTLLVGESATTFAQSMGFINEDLSTSASQA 153
Query: 141 ------------KQWQELKEKLMK--GEVRHWKKLGELIKEHPEVL----RSTVGAVAFD 182
W+ + K G + L + I H E T+G V
Sbjct: 154 LHSDWLARNCQPNYWRNVIPDPSKYCGPYKPPGILKQDIPIHKETEDDRGHDTIGMVVIH 213
Query: 183 GE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAG-ASCTGLGEVAIKLALAKTAT 240
+ AGTST G+ K+ GRVGD+PI GAG YA++ AG A+ TG G++ ++ + A
Sbjct: 214 KTGHIAAGTSTNGIKFKIHGRVGDSPIPGAGAYADDTAGAAAATGNGDILMRFLPSYQAV 273
Query: 241 DFVRLGLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKN 285
+++R G D A + I KHF + +I + G+ G A N
Sbjct: 274 EYMRRGEDPTIACQKVISRIQKHFPEFFGAVICANVTGSYGAACN 318
>sp|Q64191|ASPG_MOUSE N4-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASE PRECURSOR
(GLYCOSYLASPARAGINASE) (ASPARTYLGLUCOSAMINIDASE)
(N4-(N-ACETYL-BETA-GLUCOSAMINYL)-L-ASPARAGINE AMIDASE)
(AGA) >gi|1478357|gb|AAB36101.1| (S81393)
aspartylglucosaminidase, AGA [mice, liver, brain,
Peptide, 346 aa] [Mus sp.]
Length = 346
Score = 130 bits (324), Expect = 2e-29
Identities = 96/276 (34%), Positives = 131/276 (46%), Gaps = 22/276 (7%)
Query: 32 AGWKELKKGSALDAVEEAIKVLEDNPIFNA-GTGSVLTIDGKVEMDAAIMRGKTLEAGAV 90
A W L GSALDAVE V E G G G+ +DA IM G ++ GAV
Sbjct: 43 AWWTLLSGGSALDAVENGCAVCEKEQCDGTVGFGGSPDEGGETTLDAMIMDGTAMDVGAV 102
Query: 91 AGIWGVKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFPEYDPTTEERR---------- 140
G+ +KN I VAR+V+E T H LLVG+ A KFA MGF D +T+ R
Sbjct: 103 GGLRRIKNAIGVARRVLEHTTHTLLVGDSATKFAESMGFTNEDLSTKTSRDLHSDWLSRN 162
Query: 141 ---KQWQELKEKLMK--GEVRHWKKLGELIKEHPEVL----RSTVGAVAFDGE-EVVAGT 190
W+ + K G + L + I H E + T+G V AGT
Sbjct: 163 CQPNYWRNVIPDPSKYCGPYKPSGFLKQSISPHKEEVDIHSHDTIGMVVIHKTGHTAAGT 222
Query: 191 STGGVFLKMFGRVGDTPIIGAGTYANEVAG-ASCTGLGEVAIKLALAKTATDFVRLGLDA 249
ST G+ K+ GRVGD+PI GAG YA++ AG A+ TG G+ ++ + A +++R G D
Sbjct: 223 STNGIKFKIPGRVGDSPIPGAGAYADDTAGAAAATGDGDTLLRFLPSYQAVEYMRGGDDP 282
Query: 250 QAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKN 285
A + I K++ +I G+ G A N
Sbjct: 283 AIACQKVILRIQKYYPNFFGAVICASVNGSYGAACN 318
>gi|11446109 aspartylglucosaminidase precursor [Homo sapiens]
Length = 306
Score = 130 bits (323), Expect = 2e-29
Identities = 98/281 (34%), Positives = 137/281 (47%), Gaps = 29/281 (10%)
Query: 31 LAGWKELKKG-SALDAVEEAIKVLE----DNPIFNAGTGSVLTIDGKVEMDAAIMRGKTL 85
L W+ L G SALDAVE + E D + G+ L G+ +DA IM G T+
Sbjct: 1 LTAWRALASGGSALDAVESGCAMCEREQCDGSVGFGGSPDEL---GETTLDAMIMDGTTM 57
Query: 86 EAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFPEYDPTTEERR----- 140
+ GAV + +KN I VARKV+E T H LLVGE A FA+ MGF D +T +
Sbjct: 58 DVGAVGDLRRIKNAIGVARKVLEHTTHTLLVGESATTFAQSMGFINEDLSTTASQALHSD 117
Query: 141 --------KQWQELKEKLMK--GEVRHWKKLGELIKEHPEVL----RSTVGAVAFDGE-E 185
W+ + K G + L + I H E T+G V
Sbjct: 118 WLARNCQPNYWRNVIPDPSKYCGPYKPPGILKQDIPIHKETEDDRGHDTIGMVVIHKTGH 177
Query: 186 VVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAG-ASCTGLGEVAIKLALAKTATDFVR 244
+ AGTST G+ K+ GRVGD+PI GAG YA++ AG A+ TG G++ ++ + A +++R
Sbjct: 178 IAAGTSTNGIKFKIHGRVGDSPIPGAGAYADDTAGAAAATGNGDILMRFLPSYQAVEYMR 237
Query: 245 LGLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKN 285
G D A + I KHF + +I + G+ G A N
Sbjct: 238 RGEDPTIACQKVISRIQKHFPEFFGAVICANVTGSYGAACN 278
>dbj|BAB15302.1| (AK025969) unnamed protein product [Homo sapiens]
Length = 232
Score = 125 bits (312), Expect = 5e-28
Identities = 92/229 (40%), Positives = 123/229 (53%), Gaps = 20/229 (8%)
Query: 75 MDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFPEY-- 132
MDA+IM GK L AGAV+ + + NPI +AR VMEKT H L +GA +FA MG PE
Sbjct: 1 MDASIMDGKDLSAGAVSAVQCIANPIKLARLVMEKTPHCFLTDQGAAQFAAAMGVPEIPG 60
Query: 133 DPTTEERRKQWQELKEKLMKGEVRH--WKKLGELIKEHPEVLRSTVGAVAFDGEEVVA-G 189
+ ER K+ E KEK KG + K LG TVGAVA D + VA
Sbjct: 61 EKLVTERNKKRLE-KEKHEKGAQKTDCQKNLG------------TVGAVALDCKGNVAYA 107
Query: 190 TSTGGVFLKMFGRVGDTPIIGAGTYA-NEVAGASCTGLGEVAIKLALAKTATDFVRLGLD 248
TSTGG+ KM GRVGD+P +GAG YA N++ S TG GE +K+ LA+ + G
Sbjct: 108 TSTGGIVNKMVGRVGDSPCLGAGGYADNDIGAVSTTGHGESILKVNLARLTLFHIEQGKT 167
Query: 249 AQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKNTKHMSYAFMKEG 297
+ A++ ++ K K G+I+V G+ + M +A K+G
Sbjct: 168 VEEAADLSLGY-MKSRVKGLGGLIVVSKTGDWVAKWTSTSMPWAAAKDG 215
>gi|8923202 hypothetical protein FLJ20212 [Homo sapiens]
>gi|7020161|dbj|BAA91018.1| (AK000219) unnamed protein
product [Homo sapiens]
Length = 420
Score = 121 bits (301), Expect = 9e-27
Identities = 96/289 (33%), Positives = 141/289 (48%), Gaps = 33/289 (11%)
Query: 4 IIVHGGAGTIRKEERIPKVLEGVREAVLAGWKELKKGS-ALDAVEEAIKVLEDNPIFNAG 62
++VH GAG E + + + A ++L+ G+ A DAV A+ LED+P NAG
Sbjct: 44 VLVHAGAG-YHSESKAKEYKHVCKRACQKAIEKLQAGALATDAVTAALVELEDSPFTNAG 102
Query: 63 TGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVM----------EKTDH 112
GS L + G++E DA+IM GK+L GAV + G+KNP+SVA +++ +
Sbjct: 103 MGSNLNLLGEIECDASIMDGKSLNFGAVGALSGIKNPVSVANRLLCEGQKGKLSAGRIPP 162
Query: 113 VLLVGEGAVKFARIMGFPEYDP---TTE------ERRKQWQELKEKLMKGEVRHWKKLGE 163
LVGEGA ++A G P P TT +R K+ EL E++ ++ K+
Sbjct: 163 CFLVGEGAYRWAVDHGIPSCPPNIMTTRFSLAAFKRNKRKLELAERVDTDFMQLKKRRQS 222
Query: 164 LIKEHPEVLRSTVGAVAFDGE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEV---- 218
KE+ TVGAV D E V A S+GG+ LK GRVG + G G +A
Sbjct: 223 SEKENDSGTLDTVGAVVVDHEGNVAAAVSSGGLALKHPGRVGQAALYGCGCWAENTGAHN 282
Query: 219 ---AGASCTGLGEVAIKLALAKTATDFVRLGLDAQAASEAAIELATKHF 264
S +G GE ++ LA+ + L A+ A +A +E F
Sbjct: 283 PYSTAVSTSGCGEHLVRTILARECSH----ALQAEDAHQALLETMQNKF 327
>dbj|BAB15209.1| (AK025671) unnamed protein product [Homo sapiens]
Length = 420
Score = 121 bits (301), Expect = 9e-27
Identities = 96/289 (33%), Positives = 141/289 (48%), Gaps = 33/289 (11%)
Query: 4 IIVHGGAGTIRKEERIPKVLEGVREAVLAGWKELKKGS-ALDAVEEAIKVLEDNPIFNAG 62
++VH GAG E + + + A ++L+ G+ A DAV A+ LED+P NAG
Sbjct: 44 VLVHAGAG-YHSESKAKEYKHVCKRACQKAIEKLQAGALATDAVTAALVELEDSPFTNAG 102
Query: 63 TGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVM----------EKTDH 112
GS L + G++E DA+IM GK+L GAV + G+KNP+SVA +++ +
Sbjct: 103 MGSNLNLLGEIECDASIMDGKSLNFGAVGALSGIKNPVSVANRLLCEGQKGKLSAGRIPP 162
Query: 113 VLLVGEGAVKFARIMGFPEYDP---TTE------ERRKQWQELKEKLMKGEVRHWKKLGE 163
LVGEGA ++A G P P TT +R K+ EL E++ ++ K+
Sbjct: 163 CFLVGEGAYRWAVDHGIPSCPPNIMTTRFSLAAFKRNKRKLELAERVDTDFMQLKKRRQS 222
Query: 164 LIKEHPEVLRSTVGAVAFDGE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEV---- 218
KE+ TVGAV D E V A S+GG+ LK GRVG + G G +A
Sbjct: 223 SEKENDSGTLDTVGAVVVDHEGNVAAAVSSGGLALKHPGRVGQAALYGCGCWAENTGAHN 282
Query: 219 ---AGASCTGLGEVAIKLALAKTATDFVRLGLDAQAASEAAIELATKHF 264
S +G GE ++ LA+ + L A+ A +A +E F
Sbjct: 283 PYSTAVSTSGCGEHLVRTILARECSH----ALQAEDAHQALLETMQNKF 327
>gi|11421701 hypothetical protein FLJ20212 [Homo sapiens]
Length = 423
Score = 121 bits (301), Expect = 9e-27
Identities = 96/289 (33%), Positives = 141/289 (48%), Gaps = 33/289 (11%)
Query: 4 IIVHGGAGTIRKEERIPKVLEGVREAVLAGWKELKKGS-ALDAVEEAIKVLEDNPIFNAG 62
++VH GAG E + + + A ++L+ G+ A DAV A+ LED+P NAG
Sbjct: 47 VLVHAGAG-YHSESKAKEYKHVCKRACQKAIEKLQAGALATDAVTAALVELEDSPFTNAG 105
Query: 63 TGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVM----------EKTDH 112
GS L + G++E DA+IM GK+L GAV + G+KNP+SVA +++ +
Sbjct: 106 MGSNLNLLGEIECDASIMDGKSLNFGAVGALSGIKNPVSVANRLLCEGQKGKLSAGRIPP 165
Query: 113 VLLVGEGAVKFARIMGFPEYDP---TTE------ERRKQWQELKEKLMKGEVRHWKKLGE 163
LVGEGA ++A G P P TT +R K+ EL E++ ++ K+
Sbjct: 166 CFLVGEGAYRWAVDHGIPSCPPNIMTTRFSLAAFKRNKRKLELAERVDTDFMQLKKRRQS 225
Query: 164 LIKEHPEVLRSTVGAVAFDGE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEV---- 218
KE+ TVGAV D E V A S+GG+ LK GRVG + G G +A
Sbjct: 226 SEKENDSGTLDTVGAVVVDHEGNVAAAVSSGGLALKHPGRVGQAALYGCGCWAENTGAHN 285
Query: 219 ---AGASCTGLGEVAIKLALAKTATDFVRLGLDAQAASEAAIELATKHF 264
S +G GE ++ LA+ + L A+ A +A +E F
Sbjct: 286 PYSTAVSTSGCGEHLVRTILARECSH----ALQAEDAHQALLETMQNKF 330
>gb|AAF58918.1| (AE003832) CG1827 gene product [Drosophila melanogaster]
Length = 393
Score = 120 bits (298), Expect = 2e-26
Identities = 86/241 (35%), Positives = 119/241 (48%), Gaps = 31/241 (12%)
Query: 71 GKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFP 130
G+ +DA +M G T++ GAVAG+ +K+ I VAR V+E T H +LVG+ A FA MGF
Sbjct: 116 GETTLDAMVMDGATMDVGAVAGLRRIKDAIKVARHVLEHTQHTMLVGDAASAFANAMGFE 175
Query: 131 EYDPTTEERRKQWQELKE----------------------KLMKGEVRHWKKLGELIKEH 168
T E + W + K + WK+ + +
Sbjct: 176 SESLVTPESKDMWLQWTAENCQPNFWKNVHPDPKVSCGPYKPRPTPLTRWKE--DRARNE 233
Query: 169 PEVLRS---TVGAVAFDGEEVV-AGTSTGGVFLKMFGRVGDTPIIGAGTYA-NEVAGASC 223
E+ R T+G +A D E + AGTST G K+ GRVGD+PI GAG YA NEV A
Sbjct: 234 YEIGRKNHDTIGMIAIDVESNIHAGTSTNGARHKIPGRVGDSPIPGAGAYADNEVGAAVA 293
Query: 224 TGLGEVAIKLALAKTATDFVRLGLDAQAASEAAIELATKHFGKDTMG-IIMVDSRGNVGF 282
TG G+V ++ + A + +R G A++ + KH KD MG +I VD GN G
Sbjct: 294 TGDGDVMMRFLPSLLAVETMRAGKPPAEAAQEGLRRILKHH-KDFMGALIAVDRLGNYGA 352
Query: 283 A 283
A
Sbjct: 353 A 353
>dbj|BAB08998.1| (AB012239) glycosylasparaginase-like protein [Arabidopsis thaliana]
Length = 376
Score = 107 bits (265), Expect = 2e-22
Identities = 93/323 (28%), Positives = 142/323 (43%), Gaps = 37/323 (11%)
Query: 15 KEERIPKVLEG--VREAVLAGWKELKKGS-ALDAVEEAIKVLEDNPIFNA-GTGSVLTID 70
K ++ P V+ EAV A W+ + GS A++AV E E+ G G +
Sbjct: 46 KSDKFPVVVSTWPFLEAVRAAWRAVDNGSSAVEAVVEGCSACEELRCDGTVGPGGSPDEN 105
Query: 71 GKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFP 130
G+ +DA +M G T+E GAVA + VK+ I A VM+ + H LL GEGA FA MG P
Sbjct: 106 GETMIDALVMDGVTMEVGAVAAMRYVKDGIRAAHLVMKYSQHTLLAGEGASAFAISMGLP 165
Query: 131 -EYDPTTEERRKQWQELKEKLMKGEVRH----------WK-----------------KLG 162
+ ++ E K+W + KE + R +K ++G
Sbjct: 166 GPMNLSSPESVKKWSDWKENQCQPNFRKNVVPANDCGPYKPNNSAMNVFVDKSTESCEMG 225
Query: 163 ELIKEHPEV---LRSTVGAVAFDG-EEVVAGTSTGGVFLKMFGRVGDTPIIGAGTYA-NE 217
+ + P V T+ D + GTST G K+ GRVGD PI+G+ YA +E
Sbjct: 226 AIEYKPPLVGPHNHDTISMAVIDRMGHIAVGTSTNGATYKIPGRVGDGPIVGSSAYADDE 285
Query: 218 VAGASCTGLGEVAIKLALAKTATDFVRLGLDAQAASEAAIELATKHFGKDTMGIIMVDSR 277
V G TG G+ ++ + +R G+ + A++ AI + F ++ VD
Sbjct: 286 VGGCGATGDGDTMMRFLPCYQVVESMRQGMKPEEAAKDAISRIARKFPDFVGAVVAVDKN 345
Query: 278 GNVGFAKNTKHMSYAFMKEGMNE 300
G+ A Y+ MN+
Sbjct: 346 GSHAGACYGWTFQYSVQNPDMND 368
>sp|Q21697|ASPG_CAEEL PUTATIVE N4-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASE PRECURSOR
(GLYCOSYLASPARAGINASE) (ASPARTYLGLUCOSAMINIDASE)
(N4-(N-ACETYL-BETA-GLUCOSAMINYL)-L-ASPARAGINE AMIDASE)
(AGA) >gi|7506249|pir||T30965 hypothetical protein
R04B3.2 - Caenorhabditis elegans
>gi|1213550|gb|AAA91260.1| (U50198) contains similarity
to Pfam domain PF01112 (Asparaginase_2), Score=769.1,
E-value=5.9e-228, N=1 [Caenorhabditis elegans]
Length = 378
Score = 104 bits (257), Expect = 1e-21
Identities = 79/249 (31%), Positives = 114/249 (45%), Gaps = 36/249 (14%)
Query: 67 LTIDGKVEMDAAIMRG----KTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVK 122
L ID V ++ + GAVA + +++ VA VM T H LLVGE A +
Sbjct: 88 LVIDASVSSSMVVIENIFCRDGMRVGAVANLHRIRDAARVAWGVMNFTKHTLLVGESATQ 147
Query: 123 FARIMGFPEYDPTTEERRKQWQELKEKLMKGEVRHWKKLG-------------------- 162
FA+ +GF E D +TEE K W K K K + WK +
Sbjct: 148 FAKTLGFKEEDLSTEET-KSWIS-KWKTEKCQPNFWKNVSPDPSSSCGPYKTNPLTKSMR 205
Query: 163 --ELIKEHPEV-------LRSTVGAVAFDGEEVV-AGTSTGGVFLKMFGRVGDTPIIGAG 212
L+ + E T+G V D E + AGTS+ G K+ GRVGD+PI GAG
Sbjct: 206 YYSLVNQSDEAGYLVEKTNHDTIGMVVRDTENIFSAGTSSNGARFKIPGRVGDSPIPGAG 265
Query: 213 TYANEVAGASCTGLGEVAIKLALAKTATDFVRLGLDAQAASEAAIELATKHFGKDTMGII 272
YAN+ GA+ TG G+V ++ + A + LG A+ AI K F K + ++
Sbjct: 266 AYANKFGGAAATGDGDVMMRFLPSFFAVTQMELGTKPSKAAYKAITRILKVFPKFSGAVV 325
Query: 273 MVDSRGNVG 281
++ +G +G
Sbjct: 326 AMNVKGRIG 334
>pdb|1AYY|C Chain C, Glycosylasparaginase >gi|3212474|pdb|1AYY|A Chain A,
Glycosylasparaginase >gi|5542508|pdb|2GAC|A Chain A,
T152c Mutant Glycosylasparaginase From Flavobacterium
Meningosepticum >gi|5542510|pdb|2GAC|C Chain C, T152c
Mutant Glycosylasparaginase From Flavobacterium
Meningosepticum >gi|5542512|pdb|2GAW|A Chain A, Wild
Type Glycosylasparaginase From Flavobacterium
Meningosepticum >gi|5542514|pdb|2GAW|C Chain C, Wild
Type Glycosylasparaginase From Flavobacterium
Meningosepticum
Length = 151
Score = 97.9 bits (240), Expect = 1e-19
Identities = 56/119 (47%), Positives = 78/119 (65%), Gaps = 3/119 (2%)
Query: 29 AVLAGWKELKKGS-ALDAVEEAIKVLEDNPIFNA-GTGSVLTIDGKVEMDAAIMRGKTLE 86
A + WK L KG ALDAVE+ ++++ED+P + G G DG+V +DA IM +
Sbjct: 17 ANVEAWKVLSKGGKALDAVEKGVRLVEDDPTERSVGYGGRPDRDGRVTLDACIM-DENYN 75
Query: 87 AGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFPEYDPTTEERRKQWQE 145
G+VA + +KNPISVAR VMEKT HV+LVG+GA++FA GF + + T E K+W+E
Sbjct: 76 IGSVACMEHIKNPISVARAVMEKTPHVMLVGDGALEFALSQGFKKENLLTAESEKEWKE 134
>pir||T01223 hypothetical protein F6N23.5 - Arabidopsis thaliana
>gi|3047103|gb|AAC13614.1| (AF058919) regions of weak
similarity to l-asparaginase [Arabidopsis thaliana]
>gi|7267398|emb|CAB80868.1| (AL161472) putative
glycosylasparaginase [Arabidopsis thaliana]
Length = 402
Score = 91.3 bits (223), Expect = 1e-17
Identities = 89/292 (30%), Positives = 129/292 (43%), Gaps = 70/292 (23%)
Query: 4 IIVHGGAG--TIRKEERIPKVLEGVREAVLAGWKELKK--GSALDAVEEAIKVLEDNPIF 59
+ VH GAG E+ + V+ R A LA L++ G +DAV AI+VLED+P
Sbjct: 14 VAVHVGAGYHAAANEKALRSVM---RRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 70
Query: 60 NAGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEK--TDHV---- 113
NAG GS LT DG VE DA++M G + G V + GV+N I +A ++++ T
Sbjct: 71 NAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKEQITGSTLLGR 130
Query: 114 ---------------LLVGEGAVKFARIMG------FPEYDP--TTEERRKQWQELKEKL 150
LLVGEGA ++ + E D TE R QW+ K
Sbjct: 131 IPPIVGLNFLICLPRLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRF--KA 188
Query: 151 MKGEVRHWKKLGELIKEHP---------------------EVLRSTVGAVAFDGE-EVVA 188
M EV L +EHP + + TVG + D E +
Sbjct: 189 MLSEVGAKSILS--AEEHPRGTENNETCEENVSSCAAADEDKIMDTVGVICVDNEGHIAC 246
Query: 189 GTSTGGVFLKMFGRVGDTPIIGAGTYANE--------VAGASCTGLGEVAIK 232
G+S+GG+ +K+ GRVG G+G +A+ + G +G GE ++
Sbjct: 247 GSSSGGIAMKISGRVGLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMR 298
>emb|CAB90154.1| (AL355013) similar to L-asparaginase [Schizosaccharomyces pombe]
Length = 285
Score = 86.6 bits (211), Expect = 3e-16
Identities = 81/249 (32%), Positives = 125/249 (49%), Gaps = 40/249 (16%)
Query: 4 IIVHGGAGTIRKEERIPKVLEGVREAVLAGWKELKKG-SALDAVEEAIKVLEDNPIFNAG 62
+I+H GAG + I + + +A A + LK G SAL A +A K++ED+P+ NAG
Sbjct: 6 VILHAGAGLYSGQREI-QAKKTCSDACKAAIQALKVGQSALSAAIQAAKIMEDSPVTNAG 64
Query: 63 TGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNP------ISVARKVMEKTDHV--- 113
GS L IDGKVE +A +M ++ +VA ++P I RKVM + V
Sbjct: 65 VGSNLNIDGKVECEAGVMDSESGLTASVACCNCCRHPSEACLYILNKRKVMSQHGLVPPA 124
Query: 114 LLVGEGAVKF---ARIMGFPEYDPTTEERRKQWQELKEKLMKGEVRHWKKLGELIKEHPE 170
+LVG G K + I PE T E+ MK +++ WK E++ ++P
Sbjct: 125 MLVGNGIEKLLLHSNIKLVPESHLIT-----------ERSMKTQIK-WK---EILYQNPI 169
Query: 171 VLRS--TVGAVAFD-GEEVVAGTSTGGVFLKMFGRVGDTPIIGAG----TYANEVAGASC 223
L S T+G + D + +S+GG+ LK GR+G +PI G G ++ N+ ++C
Sbjct: 170 NLSSQDTIGVICVDKNGRIAVVSSSGGLLLKPAGRIGSSPIPGHGFWIESFDNKSHSSTC 229
Query: 224 ----TGLGE 228
+G GE
Sbjct: 230 AVATSGTGE 238
>gb|AAF49544.1| (AE003528) CG5241 gene product [Drosophila melanogaster]
Length = 298
Score = 85.8 bits (209), Expect = 6e-16
Identities = 69/195 (35%), Positives = 97/195 (49%), Gaps = 26/195 (13%)
Query: 69 IDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKV---------MEKTDHVLLVGEG 119
+DG V+ DAAIM G TL GA + VKNPI +AR++ +E+ ++L G G
Sbjct: 1 MDGSVQCDAAIMDGSTLNFGACTNVSRVKNPIQLARRICDAQSSPQLLERIPPMILAGTG 60
Query: 120 AVKFARIMGFPEYDP---TTEERRKQWQELKEKL-MKGEVRHWKKLGELIKEHPE----- 170
A +A +G +P + + + Q+ K K + R K E + PE
Sbjct: 61 AEHYADEVGCSMVEPGVLISSKAKFQFNHYKSKYDLVVNSRLGKATSEESVQVPEPGNEV 120
Query: 171 ---VLRSTVGAVAFDGE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYA---NEVAGASC 223
TVGAV DG AG S+GG+ LK+ GRVG GAG +A +E+A A+C
Sbjct: 121 ELAAALDTVGAVCVDGAGNTAAGCSSGGILLKVPGRVGQAATYGAGCWATDTDELAIATC 180
Query: 224 -TGLGEVAIKLALAK 237
TG GE +K LA+
Sbjct: 181 TTGNGEYLMKTLLAR 195
>pir||T49437 asparaginase related protein [imported] - Neurospora crassa
>gi|7800880|emb|CAB91238.1| (AL355926) asparaginase
related protein [Neurospora crassa]
Length = 606
Score = 80.0 bits (194), Expect = 3e-14
Identities = 65/171 (38%), Positives = 88/171 (51%), Gaps = 19/171 (11%)
Query: 1 MVAIIVHGGAG--TIRKEERIPKVLEGVREAVLAGWKELKKG-SALDAVEEAIKVLEDNP 57
+ AI VH GAG +I E+ LE EA + LK G SA +AVE AI+ LED
Sbjct: 51 VAAIFVHAGAGYHSITNEK---VHLEACSEASRVAMRFLKAGASAPEAVEAAIRCLEDKE 107
Query: 58 IFNAGTGSVLTIDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVL--- 114
I NAG GS L +DG VE DA ++ +GA + G+KNPIS+A+ +++K+ L
Sbjct: 108 ITNAGFGSNLNMDGVVECDATVV-DHLGRSGACGAVSGIKNPISLAKLILDKSSQPLSLR 166
Query: 115 ------LVGEGAVKFARIMGF---PEYDPTTEERRKQWQELKEKLMKGEVR 156
LVG GA FA G P ++ + ++ KE L K E R
Sbjct: 167 RVPPNILVGLGARDFAEEHGMATVPNEYLISKNAKDRFLRWKEDLKKAEAR 217
>pdb|1APY|A Chain A, Human Aspartylglucosaminidase >gi|1942817|pdb|1APY|C Chain
C, Human Aspartylglucosaminidase >gi|1942819|pdb|1APZ|A
Chain A, Human Aspartylglucosaminidase Complex With
Reaction Product >gi|1942821|pdb|1APZ|C Chain C, Human
Aspartylglucosaminidase Complex With Reaction Product
Length = 162
Score = 78.8 bits (191), Expect = 7e-14
Identities = 50/115 (43%), Positives = 64/115 (55%), Gaps = 8/115 (6%)
Query: 27 REAVLAGWKELKKG-SALDAVEEAIKVLE----DNPIFNAGTGSVLTIDGKVEMDAAIMR 81
+ A A W+ L G SALDAVE + E D + G+ L G+ +DA IM
Sbjct: 14 KNATEAAWRALASGGSALDAVESGCAMCEREQCDGSVGFGGSPDEL---GETTLDAMIMD 70
Query: 82 GKTLEAGAVAGIWGVKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFPEYDPTT 136
G T++ GAV + +KN I VARKV+E T H LLVGE A FA+ MGF D +T
Sbjct: 71 GTTMDVGAVGDLRRIKNAIGVARKVLEHTTHTLLVGESATTFAQSMGFINEDLST 125
>pdb|1APY|B Chain B, Human Aspartylglucosaminidase >gi|1942818|pdb|1APY|D Chain
D, Human Aspartylglucosaminidase >gi|1942820|pdb|1APZ|B
Chain B, Human Aspartylglucosaminidase Complex With
Reaction Product >gi|1942822|pdb|1APZ|D Chain D, Human
Aspartylglucosaminidase Complex With Reaction Product
Length = 141
Score = 73.4 bits (177), Expect = 3e-12
Identities = 42/113 (37%), Positives = 63/113 (55%), Gaps = 2/113 (1%)
Query: 175 TVGAVAFDGE-EVVAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAG-ASCTGLGEVAIK 232
T+G V + AGTST G+ K+ GRVGD+PI GAG YA++ AG A+ TG G++ ++
Sbjct: 1 TIGMVVIHKTGHIAAGTSTNGIKFKIHGRVGDSPIPGAGAYADDTAGAAAATGNGDILMR 60
Query: 233 LALAKTATDFVRLGLDAQAASEAAIELATKHFGKDTMGIIMVDSRGNVGFAKN 285
+ A +++R G D A + I KHF + +I + G+ G A N
Sbjct: 61 FLPSYQAVEYMRRGEDPTIACQKVISRIQKHFPEFFGAVICANVTGSYGAACN 113
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.316 0.135 0.381
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 110599839
Number of Sequences: 2977
Number of extensions: 4663233
Number of successful extensions: 13535
Number of sequences better than 1.0e-10: 42
Number of HSP's better than 0.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 19
Number of HSP's that attempted gapping in prelim test: 13407
Number of HSP's gapped (non-prelim): 69
length of query: 305
length of database: 189,106,746
effective HSP length: 58
effective length of query: 247
effective length of database: 154,394,500
effective search space: 38135441500
effective search space used: 38135441500
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 165 (68.7 bits)