BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0157 (PAB0157) DE:NIFS PROTEIN
(401 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||E75213 nifs protein PAB0157 - Pyrococcus abyssi (strain Ors... 812 0.0
pir||G69051 nifS protein - Methanobacterium thermoautotrophicum ... 357 1e-97
pir||F70019 nifS protein homolog yurW - Bacillus subtilis >gi|26... 335 4e-91
pir||T35993 probable aminotransferase - Streptomyces coelicolor ... 310 1e-83
pir||S72761 nitrogen fixation protein homolog nifS - Mycobacteri... 302 4e-81
gb|AAG20545.1| (AE005125) NifS protein, class-V aminotransferase... 293 2e-78
pir||S76601 hypothetical protein - Synechocystis sp. (strain PCC... 289 5e-77
pir||C70872 hypothetical protein Rv1464 - Mycobacterium tubercul... 288 8e-77
gb|AAF82074.1|AF232751_2 (AF232751) nifS-like protein [Mycobacte... 279 4e-74
pir||D70110 aminotransferase (nifS) homolog - Lyme disease spiro... 277 2e-73
pir||T44909 nifS-like protein [imported] - Mycobacterium leprae ... 272 4e-72
pir||A72506 probable tRNA splicing protein SPL1 APE2023 - Aeropy... 271 1e-71
pir||D82676 aminotransferase XF1473 [imported] - Xylella fastidi... 270 3e-71
emb|CAC17128.1| (AJ301654) cysteine desulfurase [Pectobacterium ... 266 2e-70
pir||D72260 aminotransferase, class V - Thermotoga maritima (str... 265 7e-70
gb|AAF22900.1|AC006932_17 (AC006932) T27G7.17 [Arabidopsis thali... 263 3e-69
pir||H64925 aminotransferase nifS homolog b1680, pyridoxal phosp... 262 5e-69
pdb|1C0N|A Chain A, Csdb Protein, Nifs Homologue 257 2e-67
pir||F82092 aminotransferase, class V VC2309 [imported] - Vibrio... 254 2e-66
pir||F83187 probable pyridoxal-phosphate dependent enzyme PA3667... 242 6e-63
dbj|BAA75914.1| (AB024602) NIFS-like protein [Pseudomonas putida] 240 2e-62
gb|AAF73526.1| (AE002273) aminotransferase, class V [Chlamydia m... 237 1e-61
pir||C71483 probable nifS-related protein - Chlamydia trachomati... 233 4e-60
pir||G72047 nifs-related aminotransferase - Chlamydophila pneumo... 230 2e-59
pir||D82890 nitrogen fixation protein class-V pyridoxal-phosphat... 230 2e-59
pir||C71303 probable nitrogen fixation protein (nifS-1) - syphil... 229 4e-59
pir||F65063 hypothetical protein b2810 - Escherichia coli (strai... 216 3e-55
dbj|BAB07775.1| (AP001520) aminotransferase required for NAD bio... 208 9e-53
dbj|BAB07188.1| (AP001518) nitrogen fixation protein [Bacillus h... 206 3e-52
pir||I64114 nifS protein homolog HI1295 - Haemophilus influenzae... 195 9e-49
sp|P75298|NISH_MYCPN NIFS-LIKE PROTEIN >gi|2146475|pir||S73681 n... 172 7e-42
sp|Q49420|NISH_MYCGE NIFS-LIKE PROTEIN >gi|1361731|pir||B64237 n... 170 3e-41
pir||T35528 probable aminotransferase - Streptomyces coelicolor ... 153 3e-36
emb|CAA05909.1| (AJ003152) cysteine desulphurase [Ruminococcus f... 141 1e-32
gb|AAG01802.1|AF276772_1 (AF276772) cysteine desulfurase NifS [M... 138 2e-31
pir||H70364 iron-sulfur cofactor synthesis protein nifS - Aquife... 137 2e-31
sp|P25374|SPL1_YEAST TRNA SPLICING PROTEIN SPL1 (NIFS-LIKE 54.5 ... 136 3e-31
gb|AAK42385.1| NifS protein homolog (nifS) [Sulfolobus solfatari... 136 6e-31
gi|10383773 NifS-like protein; Nfs1p [Saccharomyces cerevisiae] 136 6e-31
pir||F72223 iron-sulfur cofactor synthesis protein TM1692 [simil... 135 1e-30
pdb|1ECX|B Chain B, Nifs-Like Protein >gi|7546479|pdb|1ECX|A Cha... 135 1e-30
emb|CAA07007.1| (AJ006406) AgSPL1 [Eremothecium gossypii] 132 5e-30
sp|P87185|SPL1_CANAL TRNA SPLICING PROTEIN SPL1 >gi|2121284|gb|A... 131 1e-29
pdb|1EG5|B Chain B, Nifs-Like Protein >gi|7546280|pdb|1EG5|A Cha... 130 3e-29
sp|P87187|SPL1_CANMA TRNA SPLICING PROTEIN SPL1 >gi|2121288|gb|A... 129 6e-29
pir||JC4557 capreomycin acetyltransferase (EC 2.3.1.-) - Strepto... 128 1e-28
gi|11497803 nifS protein, class-V aminotransferase (nifS-1) [Arc... 128 1e-28
pir||G82285 aminotransferase NifS, class V VC0748 [imported] - V... 128 1e-28
pir||G83168 L-cysteine desulfurase (pyridoxal phosphate-dependen... 127 2e-28
gb|AAF62329.1| (AE002486) nifS protein [Neisseria meningitidis M... 126 5e-28
pir||A65030 probable iron-sulfur cofactor synthesis protein b253... 125 7e-28
sp|P39171|NIFS_ECOLI NIFS PROTEIN HOMOLOG >gi|1799938|dbj|BAA164... 125 7e-28
pir||A83275 hypothetical protein PA2958 [imported] - Pseudomonas... 125 7e-28
sp|Q43884|NIFS_ANAAZ NIFS PROTEIN >gi|762778|gb|AAA87249.1| (L34... 125 1e-27
gi|11498172 nifS protein, class-V aminotransferase (nifS-2) [Arc... 125 1e-27
sp|P12623|NIFS_ANASP NIFS PROTEIN >gi|97706|pir||C34443 nitrogen... 124 2e-27
pir||E82231 NifS-related protein VC1184 [imported] - Vibrio chol... 123 3e-27
gb|AAA93018.1| (U49859) NifS2 [Anabaena variabilis] 122 1e-26
dbj|BAB04979.1| (AP001511) Fe-S cluster formation protein [Bacil... 121 1e-26
pir||S76131 nitrogenase cofactor synthesis protein nifS - Synech... 121 2e-26
gb|AAC49936.1| (AF000116) tRNA splicing protein [Candida maltosa] 121 2e-26
pir||E69981 iron-sulfur cofactor synthesis protein yrvO - Bacill... 120 3e-26
pir||E81852 NifS-like aminotranfserase NMA1594 [imported] - Neis... 120 4e-26
sp|P55690|NIFS_RHISN NIFS PROTEIN >gi|2182702|gb|AAB91919.1| (AE... 119 5e-26
pir||C71863 probable aminotransferase - Helicobacter pylori (str... 118 1e-25
pir||T05896 probable iron-sulfur cofactor synthesis protein F6H1... 118 1e-25
pir||T44281 iron-sulfur cofactor synthesis protein iscS [importe... 117 2e-25
pir||E64570 nifS-like protein - Helicobacter pylori (strain 2669... 117 2e-25
gb|AAF53143.1| (AE003634) CG12264 gene product [Drosophila melan... 117 2e-25
pir||A71652 iron-sulfur cofactor synthesis protein RP486 - Ricke... 116 3e-25
pir||T11683 probable iron-sulfur cofactor synthesis protein NFS1... 116 6e-25
pir||H75554 NifS-related protein - Deinococcus radiodurans (stra... 116 6e-25
emb|CAC08815.1| (AJ279023) putative SPL1 tRNA splicing protein [... 115 1e-24
gi|10864079 cysteine desulfurase [Homo sapiens] >gi|4185794|gb|A... 115 1e-24
gi|11420479 cysteine desulfurase [Homo sapiens] 115 1e-24
dbj|BAB13286.1| (AP001119) hypothetical protein [Buchnera sp. APS] 114 2e-24
sp|Q10089|YAOA_SCHPO HYPOTHETICAL 48.2 KD PROTEIN C11D3.10 IN CH... 113 4e-24
gb|AAA92036.1| (U13077) capreomycin acetyltransferase [Streptomy... 111 1e-23
pir||T11709 nifS protein homolog - fission yeast (Schizosaccharo... 111 1e-23
gi|6754846 nitrogen fixation gene, yeast homolog 1 (S. cerevisia... 111 1e-23
sp|Q57337|NIFS_HAEIN NIFS PROTEIN HOMOLOG >gi|1075069|pir||D6406... 111 2e-23
gb|AAG27074.1|AF105225_10 (AF105225) NifS [Acetobacter diazotrop... 109 7e-23
gb|AAF82635.1|AF167538_2 (AF167538) NifS [Trichodesmium sp. IMS101] 108 1e-22
pir||G81350 probable aminotransferase Cj0791c [imported] - Campy... 108 1e-22
pir||S02507 nitrogenase cofactor synthesis protein nifS - Klebsi... 106 4e-22
pir||C83387 probable pyridoxal-phosphate dependent enzyme PA2062... 106 4e-22
sp|O51886|NIFS_BUCAP NIFS PROTEIN HOMOLOG >gi|2827032|gb|AAC3812... 106 5e-22
sp|P05344|NIFS_KLEPN NIFS PROTEIN >gi|479600|pir||S34847 nifS pr... 104 2e-21
pir||D64547 iron-sulfur cofactor synthesis protein - Helicobacte... 104 2e-21
pir||G64117 nifS protein homolog HI1343 - Haemophilus influenzae... 102 9e-21
pir||A71960 probable nitrogenase cofactor synthesis protein nifS... 101 1e-20
emb|CAB96039.1| (AL360055) putative aminotransferase [Streptomyc... 100 3e-20
pir||A71107 hypothetical protein PH0626 - Pyrococcus horikoshii ... 99 6e-20
gb|AAC16992.1| (AF067211) similar to aminotransferases class-V; ... 99 1e-19
sp|Q03046|CEFD_NOCLA ISOPENICILLIN N EPIMERASE >gi|419704|pir||S... 98 1e-19
pir||S37296 nifS protein - Klebsiella pneumoniae >gi|149344|gb|A... 98 1e-19
dbj|BAB06923.1| (AP001518) L-cysteine sulfurtransferase (iron-su... 98 2e-19
gb|AAC33372.1| (AF001780) NifS [Cyanothece PCC8801] 97 4e-19
pir||F69666 iron-sulfur cofactor synthesis protein nifZ - Bacill... 97 4e-19
sp|Q01179|NIFS_RHOSH NIFS PROTEIN >gi|282176|pir||C41880 nitroge... 96 7e-19
pir||D70858 probable iron-sulfur cofactor synthesis protein Rv30... 96 9e-19
sp|Q52069|NIFS_ENTAG NIFS PROTEIN >gi|1480127|emb|CAA68020.1| (X... 95 1e-18
pir||E70390 iron-sulfur cofactor synthesis protein nifS - Aquife... 95 2e-18
gb|AAA22168.1| (M17349) nifS protein [Azotobacter vinelandii] 94 3e-18
pir||T45399 iron-sulfur cofactor synthesis protein MLCB637.06 [s... 94 4e-18
pir||E75054 capreomycin acetyltransferase or nifs-like protein P... 93 5e-18
sp|P05341|NIFS_AZOVI NIFS PROTEIN >gi|420859|pir||S29757 nitroge... 92 8e-18
sp|P42253|YCBU_BACSU HYPOTHETICAL 40.8 KD PROTEIN IN PCP-LMRB IN... 91 2e-17
pir||S74526 nitrogenase cofactor synthesis protein nifS - Synech... 88 2e-16
sp|Q07177|NIFS_RHOCA NIFS PROTEIN >gi|486694|pir||S34816 nitroge... 88 2e-16
gb|AAD02935.1| (AF061964) L-cysteine/cystine lyase C-DES [Synech... 87 3e-16
sp|P31672|NIFS_LACDE NIFS PROTEIN HOMOLOG >gi|98662|pir||S16047 ... 87 3e-16
pir||S77167 L-cysteine desulfhydrase (EC 4.4.1.-) - Synechocysti... 87 3e-16
pdb|1ELU|A Chain A, Complex Between The Cystine C-S Lyase C-Des ... 87 5e-16
pir||F70695 hypothetical protein Rv3778c - Mycobacterium tubercu... 85 1e-15
sp|P70727|NIFS_AZOBR NIFS PROTEIN >gi|1597739|gb|AAC46177.1| (U2... 85 1e-15
gb|AAF36034.1| (AC024202) contains similarity to Escherichia eco... 84 2e-15
pir||H72558 hypothetical protein APE1754 - Aeropyrum pernix (str... 84 3e-15
gb|AAD17272.1| (AF119361) NifS [Frankia sp. EuIK1] 83 7e-15
sp|P38033|NIFS_BACSU NIFS PROTEIN HOMOLOG >gi|421514|pir||B47071... 80 4e-14
gb|AAF12840.1|AF203881_13 (AF203881) NifS [Zymomonas mobilis] 80 4e-14
sp|Q59072|YG78_METJA HYPOTHETICAL PROTEIN MJ1678 >gi|2129014|pir... 78 2e-13
pir||T23860 hypothetical protein R03A10.3 - Caenorhabditis elega... 76 5e-13
emb|CAA21971.1| (AL033497) unknown hypothetical protein [Candida... 76 9e-13
pir||E75569 probable aminotransferase - Deinococcus radiodurans ... 75 1e-12
pir||E83544 hypothetical protein PA0813 [imported] - Pseudomonas... 75 1e-12
sp|P18549|CEFD_STRCL ISOPENICILLIN N EPIMERASE >gi|153204|gb|AAA... 75 2e-12
pir||B75547 iron-sulfur cofactor synthesis protein DR0215 [simil... 75 2e-12
pir||B71652 iron-sulfur cofactor synthesis protein RP487 [simila... 73 4e-12
dbj|BAB04936.1| (AP001511) NAD biosynthesis [Bacillus halodurans] 71 2e-11
gb|AAF34417.1|AF172282_6 (AF172282) hypothetical protein, simila... 70 4e-11
pir||D83347 probable aminotransferase PA2394 [imported] - Pseudo... 70 4e-11
>pir||E75213 nifs protein PAB0157 - Pyrococcus abyssi (strain Orsay)
>gi|5457665|emb|CAB49156.1| (AJ248283) NIFS PROTEIN
[Pyrococcus abyssi]
Length = 401
Score = 812 bits (2075), Expect = 0.0
Identities = 401/401 (100%), Positives = 401/401 (100%)
Query: 1 MRIPEDVRKDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQ 60
MRIPEDVRKDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQ
Sbjct: 1 MRIPEDVRKDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQ 60
Query: 61 KYEESRKVVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLP 120
KYEESRKVVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLP
Sbjct: 61 KYEESRKVVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLP 120
Query: 121 WQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEVEELGKMV 180
WQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEVEELGKMV
Sbjct: 121 WQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEVEELGKMV 180
Query: 181 KEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPP 240
KEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPP
Sbjct: 181 KEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPP 240
Query: 241 LIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLV 300
LIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLV
Sbjct: 241 LIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLV 300
Query: 301 KRTTEGLDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPV 360
KRTTEGLDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPV
Sbjct: 301 KRTTEGLDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPV 360
Query: 361 MKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSLRSSQ 401
MKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSLRSSQ
Sbjct: 361 MKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSLRSSQ 401
>pir||G69051 nifS protein - Methanobacterium thermoautotrophicum (strain Delta
H) >gi|2622499|gb|AAB85866.1| (AE000901) nifS protein
[Methanobacterium thermoautotrophicum]
Length = 400
Score = 357 bits (906), Expect = 1e-97
Identities = 190/398 (47%), Positives = 264/398 (65%), Gaps = 17/398 (4%)
Query: 5 EDVRKDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEE 64
EDVR+DIPL ++ +Y D +T+ TP PV+ AM EY+ Y AN RG + L AT+K +E
Sbjct: 7 EDVRRDIPLLRDHVYLDAASTTPTPLPVVRAMTEYFKEYNANTGRGAYSLVLRATRKLQE 66
Query: 65 SRKVVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRL 124
+R+ VA FINA DEI FTKNTSE++N+VA GL F+KGD +V EHHSN LPW RL
Sbjct: 67 AREKVAGFINASSDEIVFTKNTSEAINIVAGGLR--FRKGDSVVVPNIEHHSNFLPWLRL 124
Query: 125 AKKKGLKLEFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEELGKMVKEE 183
+++G+ + ++ D+ G +D E + + +LV V H+SNALG + +V+E+G++ +
Sbjct: 125 -RERGVDVRVVKADEGGVVDPGRIEDAVDETTRLVTVTHISNALGTVQDVKEIGRIAHDV 183
Query: 184 GAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIG 243
GA+++VDAAQS+GHMEVDV+ + ADF AF GHKG +GP G G LY + + EP ++G
Sbjct: 184 GALYLVDAAQSIGHMEVDVRAIGADFAAFPGHKGTLGPVGTGFLYCSTDVQGELEPLMLG 243
Query: 244 GGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKR- 302
GGT+ DV Y L + P +FEAGT NI G IGL A I Y+ +IGI +I+K K+ +
Sbjct: 244 GGTVLDVSEDGYVLEDFPAKFEAGTLNIAGFIGLGASIDYMNRIGIRRIQKHGMKMTEEL 303
Query: 303 --TTEGLDELEIPWYG-PRNLDKHAGVVSFNVPPLHPHDVASVLDEHK-IMVRSGHHCAL 358
T +D +E YG P+N+ G++SFN+ + PHDVA +LDE I VRSGHHCA+
Sbjct: 304 HATVSSIDGIEC--YGDPQNI---YGILSFNINNMDPHDVAKILDETAGICVRSGHHCAI 358
Query: 359 PVMKRLKIN---GTVRASFHVYNSLEEVETFLGVLEEL 393
P MK L ++ GTVRAS H YN+ EE++ LEE+
Sbjct: 359 PAMKHLALHETGGTVRASIHYYNTSEEIQLLGETLEEI 396
>pir||F70019 nifS protein homolog yurW - Bacillus subtilis
>gi|2635765|emb|CAB15258.1| (Z99120) similar to NifS
protein homolog [Bacillus subtilis]
Length = 406
Score = 335 bits (851), Expect = 4e-91
Identities = 177/397 (44%), Positives = 250/397 (62%), Gaps = 14/397 (3%)
Query: 6 DVRKDIPLTQE------VIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMAT 59
D+R+ P+ + ++Y D+ ATS P+ VIE +D+YY +Y +NVHRGVH L AT
Sbjct: 5 DIREQFPILHQQVNGHDLVYLDSAATSQKPRAVIETLDKYYNQYNSNVHRGVHTLGTRAT 64
Query: 60 QKYEESRKVVADFINAE-FDEIAFTKNTSESLNLVALGLEHL-FKKGDKIVTTPYEHHSN 117
YE +R+ V FINA+ EI FTK T+ SLN+VAL K GD++V T EHH+N
Sbjct: 65 DGYEGAREKVRKFINAKSMAEIIFTKGTTTSLNMVALSYARANLKPGDEVVITYMEHHAN 124
Query: 118 LLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEEL 176
++PWQ+ K G L++I ++G + L D + + K+VAV HVSN LG ++ ++E+
Sbjct: 125 IIPWQQAVKATGATLKYIPLQEDGTISLEDVRETVTSNTKIVAVSHVSNVLGTVNPIKEM 184
Query: 177 GKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDV 236
K+ + GA+ VVD AQS HM++DV+ L DF A S HK GPTG+GVLY K +
Sbjct: 185 AKIAHDNGAVIVVDGAQSTPHMKIDVQDLDCDFFALSSHK-MCGPTGVGVLYGKKALLEN 243
Query: 237 FEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQE 296
EP GG I+ V L E P +FEAGTP I GAIGL A I ++E+IG+D+I + E
Sbjct: 244 MEPAEFGGEMIDFVGLYESTWKELPWKFEAGTPIIAGAIGLGAAIDFLEEIGLDEISRHE 303
Query: 297 RKLVKRTTEGLDELE-IPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHH 355
KL E +L+ + YGP ++ AG+V+FN+ +HPHDVA+VLD I VR+GHH
Sbjct: 304 HKLAAYALERFRQLDGVTVYGP---EERAGLVTFNLDDVHPHDVATVLDAEGIAVRAGHH 360
Query: 356 CALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEE 392
CA P+MK L + T RASF++YN+ EE++ + L++
Sbjct: 361 CAQPLMKWLDVTATARASFYLYNTEEEIDKLVEALQK 397
>pir||T35993 probable aminotransferase - Streptomyces coelicolor
>gi|5459388|emb|CAB50746.1| (AL096839) putative
aminotransferase [Streptomyces coelicolor A3(2)]
Length = 418
Score = 310 bits (787), Expect = 1e-83
Identities = 176/404 (43%), Positives = 245/404 (60%), Gaps = 16/404 (3%)
Query: 5 EDVRKDIPLTQ-------EVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQM 57
E +RKD P+ +++Y DN ATS P+ V++A+ EYY RY ANVHRGVH L++
Sbjct: 11 EAIRKDFPILDRQVHDGHKLVYLDNAATSQKPRQVLDALSEYYERYNANVHRGVHVLAEE 70
Query: 58 ATQKYEESRKVVADFINAEF-DEIAFTKNTSESLNLVALGL-----EHLFKKGDKIVTTP 111
AT YE +R VA+FINA DE+ FTKN SESLNLVA L + +IV T
Sbjct: 71 ATALYEGARDKVAEFINAPSRDEVIFTKNASESLNLVANMLGWADDPYRVDHETEIVITE 130
Query: 112 YEHHSNLLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVI 170
EHHSN++PWQ LA++ G KL + D+G LDL++ ++ I + K+V+ VSN LG
Sbjct: 131 MEHHSNIVPWQLLAQRTGAKLRWFGLTDDGRLDLSNIDEVITEKTKVVSFVLVSNILGTQ 190
Query: 171 HEVEELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYIN 230
+ VE + + +E GA+ +DA+Q+ HM +DV+ L+ADF+AF+GHK GPTGIGVL+
Sbjct: 191 NPVEAIVRRAQEVGALVCIDASQAAPHMPLDVQALQADFVAFTGHK-MCGPTGIGVLWGR 249
Query: 231 KEFFDVFEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGID 290
+E + P L GG IE V + P +FEAGTP + A+GL A I Y+ IG+D
Sbjct: 250 QELLEDLPPFLGGGEMIETVSMHSSTYAPAPHKFEAGTPPVAQAVGLGAAIDYLNSIGMD 309
Query: 291 KIEKQERKLVKRTTEGLDEL-EIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIM 349
KI E L + + L E+ ++ GP ++ +SF + +HPHDV VLDE I
Sbjct: 310 KILAHEHALTEYAVKRLLEVPDLRIIGPTTAEERGAAISFTLGDIHPHDVGQVLDEQGIA 369
Query: 350 VRSGHHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEEL 393
VR GHHCA PV R I T RASF++Y++ E++ + LE +
Sbjct: 370 VRVGHHCARPVCLRYGIPATTRASFYLYSTPAEIDALVDGLEHV 413
>pir||S72761 nitrogen fixation protein homolog nifS - Mycobacterium leprae
>gi|466883|gb|AAA17128.1| (U00013) nifS; B1496_C2_193
[Mycobacterium leprae] >gi|2398703|emb|CAB16168.1|
(Z99125) nifS-like protein [Mycobacterium leprae]
Length = 418
Score = 302 bits (766), Expect = 4e-81
Identities = 172/402 (42%), Positives = 235/402 (57%), Gaps = 17/402 (4%)
Query: 7 VRKDIPLTQEVI-------YFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMAT 59
+R D P+ + V+ Y D+ ATS P V++A E+ + VHRG H+L + AT
Sbjct: 13 IRADFPILKRVMRGGNQLAYLDSGATSQRPVQVLDAEREFLVTSNGAVHRGAHQLMEEAT 72
Query: 60 QKYEESRKVVADFINAEFDEIAFTKNTSESLNLVALGL-EHLFKK-----GDKIVTTPYE 113
YE R +A FI A DE+ FTKN +ESLNLV+ ++ F+ GD IVTT E
Sbjct: 73 DAYERGRVDIAAFIGAAADELVFTKNATESLNLVSYVFGDNRFEGTSGDGGDVIVTTELE 132
Query: 114 HHSNLLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEV 173
HH+NL+PWQ LA++ G L + D+G +DL D+ + + K+VA H SN G + V
Sbjct: 133 HHANLIPWQELARRIGATLRWYGVTDDGQIDL-DSLQLDERVKVVAFSHHSNVTGAVAPV 191
Query: 174 EELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEF 233
EL KE GA+ V+DA QSV H VD+ L DF AFSGHK +GP GIGVLY +E
Sbjct: 192 RELVARAKEVGALTVLDACQSVPHQPVDLHGLGVDFAAFSGHK-MLGPNGIGVLYARREL 250
Query: 234 FDVFEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIE 293
V P L GG IE V + P+RFEAGTP +GLAA +Y++ IG+ +E
Sbjct: 251 LSVMPPFLTGGSMIETVTMESTTYAPAPQRFEAGTPMTSQVVGLAAAARYLDAIGMKAVE 310
Query: 294 KQERKLVKRTTEGLDELE-IPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRS 352
ER+LV EGL ++ + GP++++ VSF V +H HD+ VLD+ + VR
Sbjct: 311 AHERELVAAAVEGLSRIDGVRIIGPKSMENRGSPVSFVVDGVHAHDIGQVLDDDGVAVRV 370
Query: 353 GHHCALPVMKRLKINGTVRASFHVYNSLEEVETFL-GVLEEL 393
GHHCALP+ +R + T RASF VYN+++EV+ + GVL L
Sbjct: 371 GHHCALPLHRRFALAATARASFAVYNTVDEVDRLVAGVLRAL 412
>gb|AAG20545.1| (AE005125) NifS protein, class-V aminotransferase; NifS
[Halobacterium sp. NRC-1]
Length = 415
Score = 293 bits (743), Expect = 2e-78
Identities = 164/402 (40%), Positives = 246/402 (60%), Gaps = 19/402 (4%)
Query: 5 EDVRKDIPLTQ-------EVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQM 57
E VR D P+ + +++Y DN ATS TP+PV++A+ +YY RY +NVHRG+H LSQ
Sbjct: 12 EAVRADFPILEREVAGGEDLVYLDNAATSHTPEPVVDAIADYYRRYNSNVHRGLHELSQE 71
Query: 58 ATQKYEESRKVVADFINAE-FDEIAFTKNTSESLNLVAL--GLEHLFKKGDKIVTTPYEH 114
A+ YE++ +A F+ E +E+ FTKNT+E+ NLVA GL L GD++V T EH
Sbjct: 72 ASVAYEDAHDKLAAFVGGEDREEMVFTKNTTEAENLVAFAWGLNEL-GPGDEVVLTQMEH 130
Query: 115 HSNLLPWQRLAKKKGLKLEFIEGDDEGNLDL-ADAEKKIKGAKLVAVQHVSNALGVIHEV 173
H++L+ WQ++A + G ++++I D+G+LD+ A A+ LV H+SN LG ++ V
Sbjct: 131 HASLVTWQQVADETGAEVKYIPITDDGHLDMDAAADMITDDTALVNAVHISNTLGTVNPV 190
Query: 174 EELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEF 233
EL + + GA VD AQ+ VDV+++ ADF AFSGHK +GPTGIG LY +
Sbjct: 191 GELADIAHDHGAYIFVDGAQAAPTRAVDVQEIDADFYAFSGHK-MLGPTGIGCLYGKRHL 249
Query: 234 FDVFEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIE 293
EP L GG IE V +PP +FEAGTP I I LA + Y++ IG+D I
Sbjct: 250 LAEMEPFLYGGDMIERVSYEDATWNDPPWKFEAGTPVIAQGIALAEAVDYLQDIGMDAIR 309
Query: 294 KQERKLVKRTTEGL---DELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMV 350
E L + + L D++++ YGP D+ A VVSFNV +H HD++S+L+++ + +
Sbjct: 310 AHEEALTEYAYDQLTMTDDVDV--YGPPGDDRGA-VVSFNVDGIHAHDLSSILNDYGVAI 366
Query: 351 RSGHHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEE 392
R+G HC P+ L + + RASF++YN+ +EV+ + ++E
Sbjct: 367 RAGDHCTQPLHDTLGVPASARASFYLYNTRDEVDALVAAVDE 408
>pir||S76601 hypothetical protein - Synechocystis sp. (strain PCC 6803)
>gi|1001708|dbj|BAA10545.1| (D64004) NifS [Synechocystis
sp.]
Length = 420
Score = 289 bits (731), Expect = 5e-77
Identities = 167/403 (41%), Positives = 239/403 (58%), Gaps = 18/403 (4%)
Query: 7 VRKDIP-LTQEV-----IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQ 60
VR+D P L QE+ +Y DN ATS P+ V+E + YY ANVHRG H+LS AT
Sbjct: 13 VRQDFPILNQEINGHPLVYLDNAATSQKPRAVLEKLMHYYENDNANVHRGAHQLSVRATD 72
Query: 61 KYEESRKVVADFINAEFD-EIAFTKNTSESLNLVAL--GLEHLFKKGDKIVTTPYEHHSN 117
YE R VA FINA EI +T+N +E++NLVA G+ +L K GD+I+TT EHHSN
Sbjct: 73 AYEAVRNKVAKFINARSPREIVYTRNATEAINLVAYSWGMNNL-KAGDEIITTVMEHHSN 131
Query: 118 LLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEEL 176
L+PWQ +A K G L+F++ D++ + DL + + + KLV V H+SN LG ++ EE+
Sbjct: 132 LVPWQMVAAKTGAVLKFVQLDEQESFDLEHFKTLLSEKTKLVTVVHISNTLGCVNPAEEI 191
Query: 177 GKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDV 236
++ + GA +VDA QS H +DV+ + D+L SGHK PTGIG LY +E +
Sbjct: 192 AQLAHQAGAKVLVDACQSAPHYPLDVQLIDCDWLVASGHK-MCAPTGIGFLYGKEEILEA 250
Query: 237 FEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQE 296
P GG I +V + E P +FEAGTP I AI L A + Y+ +G++ I E
Sbjct: 251 MPPFFGGGEMIAEVFFDHFTTGELPHKFEAGTPAIAEAIALGAAVDYLTDLGMENIHNYE 310
Query: 297 RKLVKRTTEGLDEL-EIPWYGPRNLDKH---AGVVSFNVPPLHPHDVASVLDEHKIMVRS 352
+L +GL ++ ++ YGP KH A + SFNV LH DVA+++D+ I +RS
Sbjct: 311 VELTHYLWQGLGQIPQLRLYGPN--PKHGDRAALASFNVAGLHASDVATMVDQDGIAIRS 368
Query: 353 GHHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVK 395
GHHC P+ + +G+ RAS + YN+ EE++ FL L+ ++
Sbjct: 369 GHHCTQPLHRLFDASGSARASLYFYNTKEEIDLFLQSLQATIR 411
>pir||C70872 hypothetical protein Rv1464 - Mycobacterium tuberculosis (strain
H37RV) >gi|2791398|emb|CAA15994.1| (AL021184)
hypothetical protein Rv1464 [Mycobacterium tuberculosis]
Length = 417
Score = 288 bits (729), Expect = 8e-77
Identities = 164/391 (41%), Positives = 223/391 (56%), Gaps = 15/391 (3%)
Query: 7 VRKDIPLTQEVI-------YFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMAT 59
+R D P+ + ++ Y D+ ATS P V++A E+ VHRG H+L + AT
Sbjct: 13 IRADFPILKRIMRGGNPLAYLDSGATSQRPLQVLDAEREFLTASNGAVHRGAHQLMEEAT 72
Query: 60 QKYEESRKVVADFINAEFDEIAFTKNTSESLNLVALGL-----EHLFKKGDKIVTTPYEH 114
YE+ R +A F+ A+ DE+ FTKN +E+LNLV+ L E GD IVTT EH
Sbjct: 73 DAYEQGRADIALFVGADTDELVFTKNATEALNLVSYVLGDSRFERAVGPGDVIVTTELEH 132
Query: 115 HSNLLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEVE 174
H+NL+PWQ LA++ G L + D+G +DL D+ K+VA H SN GV+ V
Sbjct: 133 HANLIPWQELARRTGATLRWYGVTDDGRIDL-DSLYLDDRVKVVAFTHHSNVTGVLTPVS 191
Query: 175 ELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFF 234
EL + GA+ V+DA QSV H VD+ +L DF AFSGHK +GP GIGVLY +E
Sbjct: 192 ELVSRAHQSGALTVLDACQSVPHQPVDLHELGVDFAAFSGHK-MLGPNGIGVLYGRRELL 250
Query: 235 DVFEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEK 294
P L GG IE V + P+RFEAGTP +GLAA +Y+ IG+ +E
Sbjct: 251 AQMPPFLTGGSMIETVTMEGATYAPAPQRFEAGTPMTSQVVGLAAAARYLGAIGMAAVEA 310
Query: 295 QERKLVKRTTEGLDELE-IPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSG 353
ER+LV EGL ++ + GP ++ V+F V +H HDV VLD+ + VR G
Sbjct: 311 HERELVAAAIEGLSGIDGVRILGPTSMRDRGSPVAFVVEGVHAHDVGQVLDDGGVAVRVG 370
Query: 354 HHCALPVMKRLKINGTVRASFHVYNSLEEVE 384
HHCALP+ +R + T RASF VYN+ +EV+
Sbjct: 371 HHCALPLHRRFGLAATARASFAVYNTADEVD 401
>gb|AAF82074.1|AF232751_2 (AF232751) nifS-like protein [Mycobacterium avium subsp.
paratuberculosis]
Length = 685
Score = 279 bits (706), Expect = 4e-74
Identities = 160/402 (39%), Positives = 235/402 (57%), Gaps = 19/402 (4%)
Query: 7 VRKDIPLTQE------VIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQ 60
+R D P+ +E +I+FDN AT+ P+ VI+ + +Y +N+HR H L+ AT
Sbjct: 284 IRSDFPILKETVNGKPLIWFDNAATTQKPQVVIDRLSHFYAHENSNIHRAAHELAARATD 343
Query: 61 KYEESRKVVADFINA-EFDEIAFTKNTSESLNLVA--LGLEHLFKKGDKIVTTPYEHHSN 117
YEE+R VA+FI A D I F + T+E++NLVA G +HL + GD+IV T EHH+N
Sbjct: 344 AYEEARDTVAEFIGAPSSDNIVFVRGTTEAINLVAHAWGAKHL-QPGDEIVITHLEHHAN 402
Query: 118 LLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEEL 176
++PWQ +++K G L+ DD GNL L++ E + KLVA HVSNALG + V+++
Sbjct: 403 IVPWQLISQKTGAILKVAPIDDAGNLLLSEFEGLLGPRTKLVAASHVSNALGTVMPVDKI 462
Query: 177 GKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDV 236
++ GA ++D AQS+ H+ +DV +L ADF FSGHK GPTGIG LY +E
Sbjct: 463 VELGHRYGARVLIDGAQSIQHIPIDVAELGADFFVFSGHK-IYGPTGIGALYGTEEALTE 521
Query: 237 FEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQE 296
P GG I DV L PP +FEAGT NI A+GL ++Y++++G+++I E
Sbjct: 522 TPPWQGGGHMIADVTLERSLYQGPPTKFEAGTGNIADAVGLTEALRYVQRLGVERIAAYE 581
Query: 297 RKLVKRTTEGLDELEIPWYGPR---NLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSG 353
L++ T L ++ G R + A V+SF + P +V L+ I VR+G
Sbjct: 582 HALLEYATPRLADIP----GVRLIGTAQEKASVLSFVLAGHEPLEVGKALNAEGIAVRAG 637
Query: 354 HHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVK 395
HHCA P ++RL + TVR SF YN+ EE++ FL + + +
Sbjct: 638 HHCAQPALRRLGLEATVRPSFAFYNTFEEIDVFLRAVRRIAE 679
>pir||D70110 aminotransferase (nifS) homolog - Lyme disease spirochete
>gi|2687967|gb|AAC66472.1| (AE001121) aminotransferase
(nifS) [Borrelia burgdorferi]
Length = 422
Score = 277 bits (700), Expect = 2e-73
Identities = 156/408 (38%), Positives = 245/408 (59%), Gaps = 19/408 (4%)
Query: 7 VRKDIPLTQE------VIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQ 60
+RKD P+ + +IYFDN ATS PK VI + EYY Y ANVHR H+ + ++
Sbjct: 16 LRKDFPILNKKFDNKYIIYFDNAATSQKPKNVIYSNVEYYENYNANVHRSGHKFAIQSSI 75
Query: 61 KYEESRKVVADFINAEFDE-IAFTKNTSESLNLVALGLEH--LFKKGDKIVTTPYEHHSN 117
K E++R++V +FINAE + I FT T++ +N +A + FKK D+I+ T EH+SN
Sbjct: 76 KIEKTRELVKNFINAESAKNIIFTSGTTDGINTIASSFFYSKYFKKKDEIILTTLEHNSN 135
Query: 118 LLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEEL 176
LLPW LA LK++ + ++ G + + EK I + KL+++ ++N LG I+++E +
Sbjct: 136 LLPWVNLANLANLKIKLAKFNEMGIITPEEIEKLITEKTKLISISGINNTLGTINDLESI 195
Query: 177 GKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDV 236
GK+ K+ VDAAQ H+++DVKK+ DFL FSGHK + PTGIG+LYI+ +
Sbjct: 196 GKIAKKYNICLFVDAAQMAPHIKIDVKKIGCDFLVFSGHK-MLAPTGIGILYISNNMAEK 254
Query: 237 FEPPLIGGGTIEDV----ELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKI 292
+GG T+E++ E +K ++ P +FE+GTPNI G IGL IKYI+ I +D I
Sbjct: 255 LHSSKLGGNTVEEIFIENEKIKFKASDSPNKFESGTPNIAGIIGLEEAIKYIDNISMDFI 314
Query: 293 EKQERKLVKRTTEGLDEL-EIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVR 351
+ +++L++ + L EL E+ + NL K ++SF V +H HD+ + LD I R
Sbjct: 315 LEHDKQLIEYGVKKLQELDEVEFILNTNL-KRNSIISFTVKNIHSHDIETYLDTMGIATR 373
Query: 352 SGHHCALPVM--KRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSL 397
+G C+ + L + +R SF+ YN+ EE++ F+ L++++K L
Sbjct: 374 AGRTCSYVAFFPENLNKDHLLRISFYFYNTQEEIDNFILGLKKVIKEL 421
>pir||T44909 nifS-like protein [imported] - Mycobacterium leprae
>gi|2342628|emb|CAB11409.1| (Z98741) nifS-like protein
[Mycobacterium leprae]
Length = 611
Score = 272 bits (689), Expect = 4e-72
Identities = 158/402 (39%), Positives = 234/402 (57%), Gaps = 19/402 (4%)
Query: 7 VRKDIPLTQE------VIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQ 60
VR D P+ QE +I+FDN AT+ P+ VI+ + +Y +N+HR H L+ AT
Sbjct: 210 VRADFPILQETVNGKPLIWFDNAATTQKPQVVIDRLSYFYAHENSNIHRAAHELAARATD 269
Query: 61 KYEESRKVVADFINA-EFDEIAFTKNTSESLNLVAL--GLEHLFKKGDKIVTTPYEHHSN 117
YEE+R+ FI A + EI F + T+E++NLVA G +HL + GD++V T EHH+N
Sbjct: 270 AYEEARETARRFIGAAKAQEIIFVRGTTEAINLVAYAWGGKHL-QPGDEVVITHLEHHAN 328
Query: 118 LLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEEL 176
++PWQ L+ + G L+ DD GNL +++ E + KLVA VSNALG + + E++
Sbjct: 329 IVPWQLLSSQTGAILKVAPVDDAGNLLMSEFEDLLGPRTKLVAATQVSNALGTVTQGEKI 388
Query: 177 GKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDV 236
++ GA ++D AQS+ H+ ++V +L ADF FSGHK GPTGIGVLY ++
Sbjct: 389 VELGHRYGARVLIDGAQSIPHLPINVSELGADFFVFSGHK-IYGPTGIGVLYGCEDVLTE 447
Query: 237 FEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQE 296
P GG I DV L PP +FEAGT NI A+GL ++Y+E++G+ +I E
Sbjct: 448 MPPWQGGGNMIVDVTLERSLYQGPPNKFEAGTGNIADAVGLGEALRYVERVGVQRIASHE 507
Query: 297 RKLVKRTTEGLDELEIPWYGPR---NLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSG 353
+ L+ T L ++ G R + A V+SF + P +V L+ I VR+G
Sbjct: 508 QALLDYATPRLADIP----GVRLVGTATEKASVLSFVLAGHEPLEVGKALNAEGIAVRAG 563
Query: 354 HHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVK 395
HHCA PV++RL + TVR SF YN+ EE++ F+ V+ + +
Sbjct: 564 HHCAQPVLRRLGLEATVRPSFAFYNTYEEIDVFINVVRRIAE 605
>pir||A72506 probable tRNA splicing protein SPL1 APE2023 - Aeropyrum pernix
(strain K1) >gi|5105721|dbj|BAA81033.1| (AP000063) 411aa
long hypothetical tRNA splicing protein SPL1 [Aeropyrum
pernix]
Length = 411
Score = 271 bits (685), Expect = 1e-71
Identities = 158/406 (38%), Positives = 239/406 (57%), Gaps = 15/406 (3%)
Query: 6 DVRKDIP-LTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEE 64
+VR + P L + ++Y DN A++L P+ V+EAM E+ R ANVHRGVHRLS A++ YE+
Sbjct: 8 EVRSEFPELERGIVYLDNAASTLKPRRVVEAMREFSYRSYANVHRGVHRLSMEASKAYED 67
Query: 65 SRKVVADFINAEFDEIAFTKNTSESLNLVALGLEHL-FKKGDKIVTTPYEHHSNLLPWQR 123
+ +VVA + +DE+ FT+NT+E++ L AL L + +G +++ T +HHS LLPW R
Sbjct: 68 AHEVVARLVGGSWDEVVFTRNTTEAMQLAALTLAYNGLLRGGEVLVTAADHHSTLLPWVR 127
Query: 124 LAKKKGLKLEFIEGDDEG--NLDLADAEKKIKGAKLVAVQHVSNALGVIHEVEELGKMVK 181
A+ G + + D G DL + + + + VAV HVSN GV+ VE++ K K
Sbjct: 128 AARLGGGRARILPLDGRGVPRWDLLE-DYITEDTRAVAVGHVSNVTGVVAPVEDIVKAAK 186
Query: 182 EEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPL 241
+ GA+ V+D+AQ V H+ VD +++ D AFSGHK +GPTGIGVL+ ++ + EPPL
Sbjct: 187 KVGALVVLDSAQGVPHLPVDFRRMGVDMAAFSGHK-MLGPTGIGVLWARRDLLEELEPPL 245
Query: 242 IGGGTIEDVEL----CCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQER 297
GGGT+ V L + EPP +FEAGTP I A+GLA + +IG++ + + E
Sbjct: 246 GGGGTVSRVRLQGGSVEIEWEEPPWKFEAGTPPIIEAVGLAEAANMLMEIGMEHVARHED 305
Query: 298 KLVKRTTEGLDELE----IPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSG 353
+L T + L+ L I + GP + G+VS P ++ +LD I VR+G
Sbjct: 306 ELTSHTMKLLEPLYSEGLIRYVGPDKPGERHGIVSITTHLKSPDELGLLLDRRGIAVRTG 365
Query: 354 HHCALPVMKRLKIN-GTVRASFHVYNSLEEVETFLGVLEELVKSLR 398
HCA + + + G+V ASF++YN E+ E LEE++ + R
Sbjct: 366 LHCAHILHDHVDASMGSVWASFYIYNCKEDAERLAEALEEILTTRR 411
>pir||D82676 aminotransferase XF1473 [imported] - Xylella fastidiosa (strain
9a5c) >gi|9106497|gb|AAF84282.1|AE003977_5 (AE003977)
aminotransferase [Xylella fastidiosa]
Length = 416
Score = 270 bits (682), Expect = 3e-71
Identities = 151/404 (37%), Positives = 231/404 (56%), Gaps = 16/404 (3%)
Query: 5 EDVRKDIPLTQE------VIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMA 58
E +R D PL Q +IYFDN T+ P+ VI A D +Y R+ AN+ R VH L A
Sbjct: 17 ERLRTDFPLLQRHVHGKPLIYFDNANTAQKPQAVITATDTFYRRHNANISRAVHTLGTEA 76
Query: 59 TQKYEESRKVVADFINAEFDEIAFTKNTSESLNLVALG--LEHLFKKGDKIVTTPYEHHS 116
T+ YE +R +A +NA E+ T+ ++NL+A L L + GD I+ + EHH+
Sbjct: 77 TEAYEATRTALATLLNAPTHELVLCSGTTFAINLIAYSWALPRL-RAGDVILVSRMEHHA 135
Query: 117 NLLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKG--AKLVAVQHVSNALGVIHEVE 174
N++PWQ +A++ G +++ + G LDL DA + KL+A+ HVSN LG ++ +
Sbjct: 136 NIVPWQLIAERTGARIQVADILPNGTLDL-DALHTLMTPQVKLLAITHVSNVLGTVNPIH 194
Query: 175 ELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFF 234
++ + ++ G VVD +Q+ H +D+ + DF A +GHK GPTG G L+ +E
Sbjct: 195 DICRQARQRGITTVVDGSQAAPHRHIDIPAIGCDFYAITGHK-LYGPTGTGALWARREHL 253
Query: 235 DVFEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEK 294
+ P L GG I++V PP +FEAGTPNI G IGL A + Y+ +IGI++IE
Sbjct: 254 HIMPPFLGGGEMIKEVSFDGTLFNTPPHKFEAGTPNIAGFIGLRAAVDYVRRIGIEQIET 313
Query: 295 QERKLVKRTTEGLDELE-IPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSG 353
+E +L+ TE L +++ + +G A VVSF + H HD+A++LD + +RSG
Sbjct: 314 RETELLAHLTEELQKIDGMRLFG--TAPNKAAVVSFMIEGTHAHDLATLLDLEGVAIRSG 371
Query: 354 HHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSL 397
HCA P+++ + T RAS YN+ EE+E F+ L ++ K L
Sbjct: 372 QHCAHPLLQYYGVTATCRASLAFYNTHEEIERFIAALLKVRKLL 415
>emb|CAC17128.1| (AJ301654) cysteine desulfurase [Pectobacterium chrysanthemi]
Length = 412
Score = 266 bits (674), Expect = 2e-70
Identities = 156/402 (38%), Positives = 229/402 (56%), Gaps = 15/402 (3%)
Query: 7 VRKDIPLTQEVI------YFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQ 60
VR D P+ Q+ + Y D+ A++ P VI+ ++YL A VHRG+H LS AT
Sbjct: 13 VRADFPILQQSVNGQPLAYLDSAASAQKPLAVIDRERDFYLHEYAAVHRGIHTLSARATS 72
Query: 61 KYEESRKVVADFINA-EFDEIAFTKNTSESLNLVALGLEHL-FKKGDKIVTTPYEHHSNL 118
EE R VA FI+A ++I F + T+E++NLVA F+ GD +V + EHH+N+
Sbjct: 73 AMEEVRAKVATFIHAASAEDIVFVRGTTEAINLVANSYGRTAFQPGDNLVISEMEHHANI 132
Query: 119 LPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEELG 177
+PWQ LA+ +GL L + D+G LD+A + + +LVAV VSN LG ++ + E+
Sbjct: 133 VPWQMLAQARGLTLRVLPITDDGELDMAQLPALLDERTRLVAVTQVSNVLGTVNPLAEII 192
Query: 178 KMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVF 237
+ GA +VD AQ+V H VDV+ L DF AFSGHK GP+GIGVLY E
Sbjct: 193 RQAHACGAKVLVDGAQAVMHQAVDVQALDCDFYAFSGHK-LYGPSGIGVLYGKSELLQAM 251
Query: 238 EPPLIGGGTIEDVELCC-YKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQE 296
P GG I +V L +PP RFEAG+P++ G IGL A + Y+ +G+D I+ E
Sbjct: 252 PPWEGGGAMIREVSLTQGTTYADPPWRFEAGSPHVAGIIGLGAALDYVSALGVDAIQAHE 311
Query: 297 RKLVKRTTEGLDELE-IPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHH 355
L++ L E+ + YGP + GV++FN+ H DV S LD++ I +R+GHH
Sbjct: 312 GLLMRYALASLAEVPTLRLYGPVH---RQGVIAFNLGRHHAFDVGSFLDQYGIAIRTGHH 368
Query: 356 CALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSL 397
CA+P+M R + RAS +Y+ +E++ + L + + L
Sbjct: 369 CAMPLMSRYGVPSMCRASLALYSCQDEIDRLVAGLHRIHRLL 410
>pir||D72260 aminotransferase, class V - Thermotoga maritima (strain MSB8)
>gi|4981951|gb|AAD36460.1|AE001791_22 (AE001791)
aminotransferase, class V [Thermotoga maritima]
Length = 413
Score = 265 bits (670), Expect = 7e-70
Identities = 155/397 (39%), Positives = 237/397 (59%), Gaps = 21/397 (5%)
Query: 15 QEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFIN 74
+ ++Y D+ AT+L PK V++ ++E+Y ANVHR VH L+ AT ++EE+R+ A+F+
Sbjct: 20 KRIVYLDSAATTLKPKQVVKKLEEFYYNSYANVHRAVHTLAVRATTEFEETREEFAEFLG 79
Query: 75 AEFDEIAFTKNTSESLNLVALGL--EHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKL 132
A +EI FT T+ ++NL + L + K+ D ++ + EHH+N +PW RL+K G ++
Sbjct: 80 ASPEEIIFTSGTTMAINLAVVSLLRSGILKEDDLVLVSLLEHHANFVPWLRLSKFHGYRI 139
Query: 133 EFIEGDDE-GNLDLADAEK-KIKGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVD 190
+FI G L++ D K + K K+VA+ +SN G VEEL + +I ++D
Sbjct: 140 DFIRPSGRFGTLEMDDLLKHRDKNPKVVAITGLSNVTGQRIPVEELRNVF--PNSIILLD 197
Query: 191 AAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDV 250
AQ + H V K++ DFLAFS HK +GPTG+GVLY +E + EP L GG I+ V
Sbjct: 198 GAQLIPHEPVKPKEIGVDFLAFSLHK-MLGPTGVGVLYGKREVLEQMEPFLYGGEMIDRV 256
Query: 251 ELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDEL 310
L E P +FEAGTPNI + ++Y+++ G D + ++ +L + + + ++
Sbjct: 257 SLEDVTFNELPYKFEAGTPNIADIVASREALRYLKETGFDSVHEKIEQLTQMALKEMMKI 316
Query: 311 -EIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLD-EHKIMVRSGHHCALPVMKRLK--- 365
+ YGP + +H G+VSFNV +HPHDVA +LD E I VRSGHHCA P+M LK
Sbjct: 317 GGVEIYGPLDERQH-GIVSFNVKGIHPHDVAHILDQEFGIAVRSGHHCAQPLMSILKEQS 375
Query: 366 ----INGTVRASFHVYNSLEEVETFLGVLEELVKSLR 398
N T RASF++YN+ E+V+ +L E VK ++
Sbjct: 376 QIDFPNSTCRASFYIYNTEEDVK----ILVEGVKKVK 408
>gb|AAF22900.1|AC006932_17 (AC006932) T27G7.17 [Arabidopsis thaliana]
Length = 526
Score = 263 bits (664), Expect = 3e-69
Identities = 184/469 (39%), Positives = 248/469 (52%), Gaps = 77/469 (16%)
Query: 7 VRKDIP-LTQEV-----IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQ 60
VRKD L QEV +Y D+ ATS P V++A+ YY Y +NVHRG+H LS AT
Sbjct: 57 VRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLDALQNYYEFYNSNVHRGIHYLSAKATD 116
Query: 61 KYEESRKVVADFINA-EFDEIAFTKNTSESLNLVAL--GLEHLFKKGDKIVTTPYEHHSN 117
++E +RK VA FINA + EI FT+N +E++NLVA GL +L K GD+++ T EHHS
Sbjct: 117 EFELARKKVARFINASDSREIVFTRNATEAINLVAYSWGLSNL-KPGDEVILTVAEHHSC 175
Query: 118 LLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKG-AKLVAVQHVSNALGVIH----- 171
++PWQ +++K G L+F+ +++ D+ + I KLVAV HVSN L +
Sbjct: 176 IVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELISPKTKLVAVHHVSNVLEGKYLTYVS 235
Query: 172 -------EVEELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHK-GPM-GPT 222
+EE+ + GA +VDA QSV HM VDV+KL ADFL S HK G M GPT
Sbjct: 236 FCLASSLPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHKVGIMCGPT 295
Query: 223 GIGVLYINKEFFDVFEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIK 282
GIG LY + P L GG I DV L EPP RFEAGTP IG AI L A +
Sbjct: 296 GIGFLYGKSDLLHSMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIALGAAVD 355
Query: 283 YIEKIGIDKIEKQE--------RKLVKRTTEGLDEL-EIPWYGPRNLDK-HAGVV-SFNV 331
Y+ IG+ KI + E ++ K E L L ++ YGPR + H G + SFNV
Sbjct: 356 YLSGIGMPKIHEYEILISCVIQVEIGKYLYEKLSSLPDVRIYGPRPSESVHRGALCSFNV 415
Query: 332 PPLHPHDVASVLD-----------------------------------------EHKIMV 350
LHP D+A+ LD +H + +
Sbjct: 416 EGLHPTDLATFLDQQIKQIHLDRSNVNRPPQTPTLLSHQNIHSGMKTENEYCMLQHGVAI 475
Query: 351 RSGHHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
RSGHHCA P+ + L +N + RAS + YN+ ++V+ F+ L + V S
Sbjct: 476 RSGHHCAQPLHRYLGVNASARASLYFYNTKDDVDAFIVALADTVSFFNS 524
>pir||H64925 aminotransferase nifS homolog b1680, pyridoxal phosphate-dependent
- Escherichia coli >gi|1742766|dbj|BAA15457.1| (D90811)
NifS protein. [Escherichia coli]
>gi|1742773|dbj|BAA15463.1| (D90812) NifS protein.
[Escherichia coli] >gi|1787970|gb|AAC74750.1| (AE000263)
orf, hypothetical protein [Escherichia coli K12]
Length = 406
Score = 262 bits (663), Expect = 5e-69
Identities = 157/404 (38%), Positives = 230/404 (56%), Gaps = 15/404 (3%)
Query: 5 EDVRKDIP-LTQEV-----IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMA 58
+ VR D P L++EV Y D+ A++ P VI+A E+Y A VHRG+H LS A
Sbjct: 6 DKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEAEFYRHGYAAVHRGIHTLSAQA 65
Query: 59 TQKYEESRKVVADFINAEF-DEIAFTKNTSESLNLVALGLEHL-FKKGDKIVTTPYEHHS 116
T+K E RK + FINA +E+ F + T+E +NLVA + + GD I+ + EHH+
Sbjct: 66 TEKMENVRKRASLFINARSAEELVFVRGTTEGINLVANSWGNSNVRAGDNIIISQMEHHA 125
Query: 117 NLLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEE 175
N++PWQ L + G +L I + +G L L + +L+A+ HVSN LG + + E
Sbjct: 126 NIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTLFDEKTRLLAITHVSNVLGTENPLAE 185
Query: 176 LGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFD 235
+ + + GA +VD AQ+V H VDV+ L DF FSGHK GPTGIG+LY+ +
Sbjct: 186 MITLAHQHGAKVLVDGAQAVMHHPVDVQALDCDFYVFSGHK-LYGPTGIGILYVKEALLQ 244
Query: 236 VFEPPLIGGGTIEDVELC-CYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEK 294
P GG I V L T+ P RFEAGTPN GG IGL A ++Y+ +G++ I +
Sbjct: 245 EMPPWEGGGSMIATVSLSEGTTWTKAPWRFEAGTPNTGGIIGLGAALEYVSALGLNNIAE 304
Query: 295 QERKLVKRTTEGLDEL-EIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSG 353
E+ L+ L+ + ++ YGP+N GV++FN+ H +DV S LD + I VR+G
Sbjct: 305 YEQNLMHYALSQLESVPDLTLYGPQN---RLGVIAFNLGKHHAYDVGSFLDNYGIAVRTG 361
Query: 354 HHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSL 397
HHCA+P+M + RAS +YN+ EEV+ + L+ + + L
Sbjct: 362 HHCAMPLMAYYNVPAMCRASLAMYNTHEEVDRLVTGLQRIHRLL 405
>pdb|1C0N|A Chain A, Csdb Protein, Nifs Homologue
Length = 406
Score = 257 bits (650), Expect = 2e-67
Identities = 156/402 (38%), Positives = 226/402 (55%), Gaps = 15/402 (3%)
Query: 7 VRKDIP-LTQEV-----IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQ 60
VR D P L++EV Y D+ A++ P VI+A E+Y A VH G H LS AT+
Sbjct: 8 VRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEAEFYRHGYAAVHAGAHTLSAQATE 67
Query: 61 KYEESRKVVADFINAEF-DEIAFTKNTSESLNLVALGLEHL-FKKGDKIVTTPYEHHSNL 118
K E RK + FINA +E+ F + T+E +NLVA + + GD I+ + EHH+N+
Sbjct: 68 KMENVRKRASLFINARSAEELVFVRGTTEGINLVANSWGNSNVRAGDNIIISQMEHHANI 127
Query: 119 LPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKGA-KLVAVQHVSNALGVIHEVEELG 177
+PWQ L + G +L I + +G L L A +L+A+ HVSN LG + + E+
Sbjct: 128 VPWQMLCARVGAELRVIPLNPDGTLQLETLPTLFDAATRLLAITHVSNVLGTENPLAEMI 187
Query: 178 KMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVF 237
+ + GA +VD AQ+V H VDV+ L DF FSGHK GPTGIG+LY+ +
Sbjct: 188 TLAHQHGAKVLVDGAQAVMHHPVDVQALDCDFYVFSGHK-LYGPTGIGILYVKEALLQEM 246
Query: 238 EPPLIGGGTIEDVELC-CYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQE 296
P GG I V L T+ P RFEAGTPN GG IGL A ++Y+ +G++ I + E
Sbjct: 247 PPWEGGGSMIATVSLSEGTTWTKAPWRFEAGTPNTGGIIGLGAALEYVSALGLNNIAEYE 306
Query: 297 RKLVKRTTEGLDEL-EIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHH 355
+ L+ L+ + ++ YGP+ GV++FN+ H +DV S LD + I VR+GHH
Sbjct: 307 QNLMHYALSQLESVPDLTLYGPQ---ARLGVIAFNLGAHHAYDVGSFLDNYGIAVRTGHH 363
Query: 356 CALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSL 397
CA+P+M + RAS +YN+ EEV+ + L+ + + L
Sbjct: 364 CAMPLMAYYNVPAMCRASLAMYNTHEEVDRLVTGLQRIHRLL 405
>pir||F82092 aminotransferase, class V VC2309 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9656874|gb|AAF95453.1|
(AE004301) aminotransferase, class V [Vibrio cholerae]
Length = 404
Score = 254 bits (641), Expect = 2e-66
Identities = 147/403 (36%), Positives = 224/403 (55%), Gaps = 18/403 (4%)
Query: 7 VRKDIPLTQEVI------YFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQ 60
+R P Q+++ Y D+ AT+ P+ VI+A+ YY ++ ANVHRG H L+ AT
Sbjct: 8 IRAQFPALQQIVNGNPLVYLDSAATTQKPQCVIDAISHYYSQHNANVHRGSHSLTAQATS 67
Query: 61 KYEESRKVVADFINAEFDE-IAFTKNTSESLNLVALGLEH-LFKKGDKIVTTPYEHHSNL 118
++E +R+ VA FI A + I +T+ +E+LNL+A + GD+I+ + EHH+N+
Sbjct: 68 QFEGAREQVAQFIGAPSSKNIIWTRGATEALNLIAQSYARSTLQAGDEILVSETEHHANI 127
Query: 119 LPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKG-AKLVAVQHVSNALGVIHEVEELG 177
+PWQ +A++ G K+ I G LA + + K+VA+ H++N G +E +
Sbjct: 128 VPWQMVAEQTGAKVVKIPMTTTGEFGLAAFRQLLSPRCKIVALAHITNVTGTRQPIEAVI 187
Query: 178 KMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVF 237
+ ++GAI V+D AQ + H VDV+ L ADF FSGHK P GIGVLY +
Sbjct: 188 QAAHQQGAIVVIDGAQGIVHETVDVRALDADFYVFSGHK-LYAPAGIGVLYGKTALLEAM 246
Query: 238 EPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQER 297
P GG +E V T P +FEAGTPN+ GAI LA I + + + +E
Sbjct: 247 PPWHGGGKMVEKVSFDGTTFTGLPGKFEAGTPNVAGAIALATAIDWYQSLDRAAVEAHLH 306
Query: 298 KLVKRTTEGL---DELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGH 354
+L ++ + + D++ + Y P +A V+S + +H D+A++LD+ I VR+GH
Sbjct: 307 QLQQQAYQAISQIDDIRVLGYQP-----NASVLSLVMDGVHHQDLATLLDQQGIAVRAGH 361
Query: 355 HCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSL 397
HCA P+M + GTVR SF VYNS EEVE + + + V L
Sbjct: 362 HCAHPLMDAFGVKGTVRISFGVYNSAEEVERLIAAIHKAVDLL 404
>pir||F83187 probable pyridoxal-phosphate dependent enzyme PA3667 [imported] -
Pseudomonas aeruginosa (strain PAO1)
>gi|9949829|gb|AAG07055.1|AE004786_7 (AE004786) probable
pyridoxal-phosphate dependent enzyme [Pseudomonas
aeruginosa]
Length = 401
Score = 242 bits (611), Expect = 6e-63
Identities = 148/406 (36%), Positives = 224/406 (54%), Gaps = 13/406 (3%)
Query: 1 MRIPEDVRKDIPL-----TQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLS 55
M +P R D P + + Y D+ AT+ P+ V++A++ YYL ANVHR H
Sbjct: 1 MSLPSPWRADFPAFSAFAAEGLTYLDSAATAQKPQAVLDALNGYYLGGAANVHRAQHVPG 60
Query: 56 QMATQKYEESRKVVADFINAEFD-EIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEH 114
+ AT+ +E +R VA +++A E+ FT+ T+E++NLVA GLE F+ GD+++ + EH
Sbjct: 61 ERATRAFEAARSRVAHWLHAGNPAEVLFTRGTTEAINLVAYGLERHFRPGDELLVSALEH 120
Query: 115 HSNLLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEV 173
H+NLLPWQ+LA ++GL L + D+ G +DL A I + +L+A+ +SN LG V
Sbjct: 121 HANLLPWQQLALRRGLVLRVLPLDERGVIDLEQARHIIGERTRLLAISQLSNVLGTWQPV 180
Query: 174 EELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEF 233
EL ++ +E GA +VD AQ H D+ L DF AFSGHK GP GIGVL+ +
Sbjct: 181 VELIQLARERGAWTLVDGAQGSVHGRHDLPGLGCDFYAFSGHK-LYGPDGIGVLWGRPQA 239
Query: 234 FDVFEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIE 293
+ GG + P FEAGTP + AIGL A I ++ + ++
Sbjct: 240 LEQLAHWQFGGEMVRHTGFHEASFHAAPLGFEAGTPAVSAAIGLGATIDWLATLDEAEVA 299
Query: 294 KQERKLVKRTTEGLDELE-IPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRS 352
E L R GL + + G + A + SF V +H D+A +L E I VR+
Sbjct: 300 AHEGALHARLLAGLLARDGVSLLG----EPQAALASFCVDGVHVADLAHLLGEQGIAVRA 355
Query: 353 GHHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSLR 398
GHHCA+P+++RL++ G +R S +YN +++E F L+ ++ LR
Sbjct: 356 GHHCAMPLLQRLEVPGALRVSLGLYNDADDLERFFLALDRSLELLR 401
>dbj|BAA75914.1| (AB024602) NIFS-like protein [Pseudomonas putida]
Length = 401
Score = 240 bits (606), Expect = 2e-62
Identities = 144/403 (35%), Positives = 213/403 (52%), Gaps = 13/403 (3%)
Query: 4 PEDVRKDIPLT-----QEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMA 58
P R D P Q Y D+ AT+ P+ +++A+ YY ANVHR H +A
Sbjct: 4 PSPWRADFPAIAALQRQHQTYLDSAATTQKPQALLDALSHYYGHGAANVHRAQHLPGALA 63
Query: 59 TQKYEESRKVVADFINA-EFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSN 117
TQ +E SR VA ++NA + +I FT + +LNL+A GLEH + GD+I + EHH+N
Sbjct: 64 TQAFETSRDKVAAWLNAADSRQIVFTHGATSALNLLAYGLEHRLEAGDEIAISALEHHAN 123
Query: 118 LLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEEL 176
LLPWQ+LA ++ L L + D G +D A + I +++A+ +SN LG + L
Sbjct: 124 LLPWQQLAHRRNLHLVVLPLDAHGRIDQDQALQLIGPRTRVLAISQLSNVLGTWQPLPAL 183
Query: 177 GKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDV 236
+ +GA+ VVD AQ V H D+++L DF FS HK GP G+GVLY + ++
Sbjct: 184 LAHARAQGALTVVDGAQGVVHGRQDMQQLGCDFYVFSSHK-LYGPDGVGVLYGRAQALEL 242
Query: 237 FEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQE 296
GG ++ E P FEAGTP I G IGL A + Y+ + + E
Sbjct: 243 LRHWQFGGEMVQLAEYHSASFRPAPLGFEAGTPPIAGVIGLGATLDYLASLDAHAVAAHE 302
Query: 297 RKLVKRTTEGLDELEIPWYGPRNLD-KHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHH 355
L + GL + E G R L + SF + +H D+A +L E I VR+GHH
Sbjct: 303 ASLHQHLLRGLGDRE----GVRVLGAPQTALASFVIEGVHNADIAHLLTEQGIAVRAGHH 358
Query: 356 CALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSLR 398
CA+P++K L + G +R S +YN ++V+ F L++ ++ LR
Sbjct: 359 CAMPLLKGLGLEGAIRVSLGLYNDSDDVQRFFDALDQGLELLR 401
>gb|AAF73526.1| (AE002273) aminotransferase, class V [Chlamydia muridarum]
Length = 400
Score = 237 bits (599), Expect = 1e-61
Identities = 147/405 (36%), Positives = 224/405 (55%), Gaps = 19/405 (4%)
Query: 6 DVRKDIPLTQE----VIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQK 61
+V++D P+ + IY D+ AT+ P+ VI+A+ +YY A V+R ++ S +
Sbjct: 3 NVKEDFPIFRNQKDPYIYLDSAATTHKPQCVIDAVTDYYSFSYATVNRAIYSASHDISSA 62
Query: 62 YEESRKVVADFINAEFD-EIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLP 120
Y R VA +I A +D EI FT+ T+ SLNL+A+ + G +V + EHH+N+L
Sbjct: 63 YWRVRSKVAAWIGARYDQEIVFTRGTTSSLNLLAIAANDSWLAGGTVVVSEAEHHANILS 122
Query: 121 WQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKM 179
W+ ++ G ++ + +DEG +DL+ EK +K G +LV++ HVSN G + V+E+ +
Sbjct: 123 WEIACRRSGATVKKVRVNDEGIIDLSHLEKLLKQGVQLVSLAHVSNVSGAVLPVQEVAFL 182
Query: 180 VKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEP 239
V GA+ +D AQ VG +++ DF AFSGHK PTGIGVLY +E + P
Sbjct: 183 VHRYGALLAIDGAQGVGSGPLNLSGWDVDFYAFSGHK-LYAPTGIGVLYGKRELLESL-P 240
Query: 240 PLIGGG---TIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGI---DKIE 293
P+ GGG + D E Y+ EPP RFEAGTP+I G +GL A I Y++ + D +
Sbjct: 241 PVEGGGDMVVVYDSESSRYQ--EPPLRFEAGTPHIAGVLGLGAAIDYLQALPFSVSDHLT 298
Query: 294 KQERKLVKRTTEGLDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSG 353
R L R L +I GP+ + S +P + D+ +LD I VRSG
Sbjct: 299 ALTRFLYNRL---LTIPDIQIVGPQQGTPRGCLCSIIIPGVQASDLGFLLDGKGIAVRSG 355
Query: 354 HHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSLR 398
H C+ P M R + +RAS VYN ++V +F+ LE++++S R
Sbjct: 356 HQCSQPAMARWDLGHVLRASLGVYNDQQDVISFVEALEDILRSYR 400
>pir||C71483 probable nifS-related protein - Chlamydia trachomatis (serotype D,
strain UW3/Cx) >gi|3329140|gb|AAC68282.1| (AE001339)
NifS-related enzyme [Chlamydia trachomatis]
Length = 401
Score = 233 bits (587), Expect = 4e-60
Identities = 140/408 (34%), Positives = 228/408 (55%), Gaps = 23/408 (5%)
Query: 6 DVRKDIPLTQE----VIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQK 61
+V+KD P+ + +Y D+ AT+ P+ VI+++ +YY A V+R ++ S T
Sbjct: 3 NVKKDFPIFKNQGDPYVYLDSAATTHKPQCVIDSIVDYYSSSYATVNRALYTASHDITFA 62
Query: 62 YEESRKVVADFINAEFD-EIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLP 120
+ + R V +I A++D EI FT+ T+ SLNL+A+ + G +V + EHH+NL+
Sbjct: 63 HWQVRSKVGSWIGAQYDQEIIFTRGTTSSLNLLAIAANDSWLAGGTVVISEAEHHANLVS 122
Query: 121 WQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKM 179
W+ ++ G ++ + DDEG +D + E+ +K G +LV++ HVSN G + + E+ +
Sbjct: 123 WELACQRSGATIKKVRVDDEGMVDCSHLEQLLKQGVQLVSLAHVSNVSGAVLPLPEIAHL 182
Query: 180 VKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEP 239
V A+F VD AQ VG +++ + DF AFSGHK PTGIGVLY KE + P
Sbjct: 183 VHRYEALFAVDGAQGVGKGPLNLSEWGVDFYAFSGHK-LYAPTGIGVLYGKKELLESL-P 240
Query: 240 PLIGGG---TIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGI---DKIE 293
P+ GGG + D E Y+ EPP RFEAGTP+I G +GL A I Y++ + D++
Sbjct: 241 PVEGGGDMVIVYDFEELSYQ--EPPLRFEAGTPHIAGVLGLGAAIDYLQALPFSITDRLT 298
Query: 294 KQERKLVKR--TTEGLDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVR 351
+ L ++ T G+ + GP+ + S ++P + D+ +LD I VR
Sbjct: 299 ELTHFLYEQLLTVPGIQII-----GPKQGAARGSLCSISIPGVQASDLGFLLDGRGISVR 353
Query: 352 SGHHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
SGH C+ P M R + +RAS +YN +++ F+ L++++++ RS
Sbjct: 354 SGHQCSQPAMVRWDLGHVLRASLGIYNEQQDILLFVEALKDILRAYRS 401
>pir||G72047 nifs-related aminotransferase - Chlamydophila pneumoniae (strain
CWL029) >gi|4376991|gb|AAD18828.1| (AE001651)
NifS-related Aminotransferase [Chlamydophila pneumoniae
CWL029] >gi|8163356|gb|AAF73623.1| (AE002169)
aminotransferase, class V [Chlamydophila pneumoniae
AR39] >gi|8979061|dbj|BAA98896.1| (AP002547)
NifS-related aminotransferase [Chlamydophila pneumoniae
J138]
Length = 406
Score = 230 bits (581), Expect = 2e-59
Identities = 137/412 (33%), Positives = 224/412 (54%), Gaps = 26/412 (6%)
Query: 5 EDVRKDIPL-------TQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQM 57
+++++D P+ + IY D+ AT+ P+ VI+A+ +Y A V+R ++ S+
Sbjct: 2 KNLKEDFPIFAAKAKENEPFIYLDSAATTQKPQQVIDAVANFYTSSYATVNRAIYSSSRN 61
Query: 58 ATQKYEESRKVVADFINAEFD-EIAFTKNTSESLNLVALGLEHLF-KKGDKIVTTPYEHH 115
T+ Y R+ V +++A D EI FT+ T+ LNL+A+ + L+ KG ++ + EHH
Sbjct: 62 VTEAYAAVREKVRKWVSAASDSEIVFTRGTTAGLNLLAISVNDLWIPKGGVVLVSEAEHH 121
Query: 116 SNLLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVE 174
+N+L W+ +++G ++ I D G +DL D EK + +GA+ V++ HVSN G + ++
Sbjct: 122 ANVLSWEIACRRRGSLVKKIRVHDSGLIDLDDLEKLLNEGAQFVSIPHVSNVTGCVQPLQ 181
Query: 175 ELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFF 234
++ ++V A VD AQ H+ +DV+ DF FS HK GPTGIGVLY K+
Sbjct: 182 QVAELVHRYDAYLAVDGAQGAPHLPIDVQLWDVDFYVFSSHK-IYGPTGIGVLYGKKDLL 240
Query: 235 DVFEPPLIGGGTIEDVELCCYKLTEP-----PERFEAGTPNIGGAIGLAAGIKYIEKIGI 289
D PP+ GGG + + Y P P +FEAGTPNI G +GL A + Y++ +
Sbjct: 241 DQL-PPVEGGGDM----VAIYDHQNPEYLPAPMKFEAGTPNIAGVLGLGAALDYLDGLSA 295
Query: 290 DKIEKQERKLVKRTTEGLDELEIPWY---GPRNLDKHAGVVSFNVPPLHPHDVASVLDEH 346
I +E L + L LEIP GP + ++ + HP D+ +LD
Sbjct: 296 KFIYDKEIALTTYLHKEL--LEIPGVEILGPSIEEPRGALIGMTIDGAHPLDLGFLLDLR 353
Query: 347 KIMVRSGHHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSLR 398
I VR+GH CA P M+R + +R S +YN ++++ F+ VL++ + +R
Sbjct: 354 GIAVRTGHQCAQPAMERWNVGHVLRVSLGIYNDEDDIDQFILVLQDSLDKIR 405
>pir||D82890 nitrogen fixation protein class-V pyridoxal-phosphate
aminotransferase UU454 [imported] - Ureaplasma
urealyticum >gi|6899446|gb|AAF30866.1|AE002141_12
(AE002141) nitrogen fixation protein - class-V
pyridoxal-phosphate aminotransferase [Ureaplasma
urealyticum]
Length = 402
Score = 230 bits (580), Expect = 2e-59
Identities = 140/384 (36%), Positives = 209/384 (53%), Gaps = 7/384 (1%)
Query: 15 QEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFIN 74
++VIY D++ATSL P+ VI+A+ +YY +Y N H + + E+R VA FIN
Sbjct: 15 KDVIYLDSSATSLKPQVVIDAIVDYYTKYSTNPHNTDSNFTFHTHEIMNETRANVAKFIN 74
Query: 75 AEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEF 134
A FDEI FT +ESLNL+A GL KKGD+I+ T EH SNLLPW +L G+K+ F
Sbjct: 75 ANFDEIIFTSGATESLNLIANGLRPHLKKGDEIILTYIEHASNLLPWYKLRDDLGVKIIF 134
Query: 135 IEGDDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVK--EEGAIFVVDA 191
++ L+D K I K+V+ N +G + + + K +K + + VDA
Sbjct: 135 ANQKNQFP-QLSDFLKVISPKTKVVSFASGGNLIGNVLDENLIIKNIKKIKPDVLVCVDA 193
Query: 192 AQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVE 251
QS+ H DVKK K+DF+ FS HK +GPTGIGV YI + +P GGG ++
Sbjct: 194 TQSIQHRMFDVKKCKSDFMVFSAHK-LLGPTGIGVAYIKNDLIKKMQPLKYGGGMNFSID 252
Query: 252 LCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELE 311
L Y+L + +FE GTP++ G G A +K++ IG +KI + E K+ K + L
Sbjct: 253 LNSYQLYDNYMKFEGGTPHVAGFYGFNAALKFLMDIGYNKIHEHELKITKYARDQL--AL 310
Query: 312 IPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMKRLKINGTVR 371
IP D + ++F+ + D A+ L I+VRSG CA + ++ +R
Sbjct: 311 IPEIKTYVQDATSSTITFSYNGVFCQDFANYLGSKNIIVRSGLSCAKIINNVIQTECAIR 370
Query: 372 ASFHVYNSLEEVETFLGVLEELVK 395
ASF++YN +V+ + ++E K
Sbjct: 371 ASFYIYNDFNDVDRLIKAIKEYQK 394
>pir||C71303 probable nitrogen fixation protein (nifS-1) - syphilis spirochete
>gi|3322912|gb|AAC65590.1| (AE001236) nitrogen fixation
protein (nifS-1) [Treponema pallidum]
Length = 404
Score = 229 bits (578), Expect = 4e-59
Identities = 137/400 (34%), Positives = 206/400 (51%), Gaps = 9/400 (2%)
Query: 8 RKDIPL---TQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEE 64
+ D PL + V Y D+ AT+ P PV+E + Y+ N RG H L+ + E
Sbjct: 7 KADFPLLLRSPRVHYLDSAATTQRPAPVLERVMHYHTHLNGNAGRGSHELAVESALLIEN 66
Query: 65 SRKVVADFINAE-FDEIAFTKNTSESLNLVA--LGLEHLFKKGDKIVTTPYEHHSNLLPW 121
+RK A FINA +I FTK+ +ESLN++A L L + GD+IV HH+N++PW
Sbjct: 67 TRKKTAQFINAAPTHDIVFTKSCTESLNIIAHCYALPRL-RAGDEIVLAISNHHANIVPW 125
Query: 122 QRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKGA-KLVAVQHVSNALGVIHEVEELGKMV 180
Q + + G ++++ D EGNLD+ +A+KKI+ K+V+ V NA G ++ +EL +
Sbjct: 126 QHVCRCTGATIQWLYPDAEGNLDIQEAQKKIRACTKIVSFSAVVNATGAVNPAQELTALA 185
Query: 181 KEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPP 240
+ GA+ V+D AQ++ H +V L DF FSGHK G+GVL + P
Sbjct: 186 HQVGAVVVIDGAQAMVHGVPNVADLGCDFFVFSGHK-MFSLFGVGVLCAPHTLLESMPPF 244
Query: 241 LIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLV 300
L GGG ++ V P ++E G+ N + L A I+Y E + + L
Sbjct: 245 LYGGGMVDFVTEQESVFKGAPHKYEGGSANTAAVVSLCAAIEYCESLESSAVRASVHALD 304
Query: 301 KRTTEGLDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPV 360
L+EL + G+++FNV +H HD A +L E +MVRSG HC+ P
Sbjct: 305 AALLARLEELPFLETYHARARERLGIIAFNVKNVHSHDTAHILGEEGVMVRSGDHCSKPF 364
Query: 361 MKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSLRSS 400
M L I RASF +YN++E+VE L + + + S
Sbjct: 365 MTHLSIQSCCRASFCIYNTMEDVEALTRALHAVGRIFQCS 404
>pir||F65063 hypothetical protein b2810 - Escherichia coli (strain K-12)
>gi|882705|gb|AAB40460.1| (U29581) ORF_o401 [Escherichia
coli] >gi|1789175|gb|AAC75852.1| (AE000364) orf,
hypothetical protein [Escherichia coli K12]
Length = 401
Score = 216 bits (545), Expect = 3e-55
Identities = 129/399 (32%), Positives = 216/399 (53%), Gaps = 10/399 (2%)
Query: 4 PEDVRKDIPLTQEV-IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKY 62
P R P Q+ +Y D+ AT+L P+ V+EA ++Y NVHR +Q T +Y
Sbjct: 6 PAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYSLSAGNVHRSQFAEAQRLTARY 65
Query: 63 EESRKVVADFINAEFDE-IAFTKNTSESLNLVALGLEH-LFKKGDKIVTTPYEHHSNLLP 120
E +R+ VA +NA D+ I +T+ T+ES+N+VA + GD+I+ + EHH+NL+P
Sbjct: 66 EAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYARPRLQPGDEIIVSVAEHHANLVP 125
Query: 121 WQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKG-AKLVAVQHVSNALGVIHEVEELGKM 179
W +A++ G K+ + + + D+ + I ++++A+ +SN G ++
Sbjct: 126 WLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPRSRILALGQMSNVTGGCPDLARAITF 185
Query: 180 VKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEP 239
G + +VD AQ H DV++L DF AFSGHK GPTGIGVLY E + P
Sbjct: 186 AHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAFSGHK-LYGPTGIGVLYGKSELLEAMSP 244
Query: 240 PLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKL 299
L GG + +V + P + EAGTPN+ G IGL+A ++++ I++ E R L
Sbjct: 245 WLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLADYDINQAESWSRSL 304
Query: 300 VKRTTEGLDELEIPWYGPRNLD-KHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCAL 358
+ L + G R+ + + +++F+ +H D+ ++L E+ I +R+G HCA
Sbjct: 305 ATLAEDALAKRP----GFRSFRCQDSSLLAFDFAGVHHSDMVTLLAEYGIALRAGQHCAQ 360
Query: 359 PVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSL 397
P++ L + GT+RASF YN+ +V+ + ++ ++ L
Sbjct: 361 PLLAELGVTGTLRASFAPYNTKSDVDALVNAVDRALELL 399
>dbj|BAB07775.1| (AP001520) aminotransferase required for NAD biosynthesis (NifS
protein) [Bacillus halodurans]
Length = 383
Score = 208 bits (524), Expect = 9e-53
Identities = 136/389 (34%), Positives = 213/389 (53%), Gaps = 12/389 (3%)
Query: 17 VIYFDNTATSLT-PKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINA 75
+IY D A+S PK V EAM Y AN RG HRL+ A +E+R+++ NA
Sbjct: 1 MIYLDQAASSFPKPKEVAEAMVAAVNEYGANPGRGGHRLATQARVTIDETRELLKTLFNA 60
Query: 76 EFDE-IAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEF 134
+ + F +N + +LN G FK+GD +V+T EH+S P ++L ++ GL + +
Sbjct: 61 PSKKHVWFYQNATGALNQALFGFP--FKEGDHVVSTALEHNSVRRPLEQLRERNGLVITY 118
Query: 135 IEGDDEGNLDLAD-AEKKIKGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQ 193
+ D+EG + A+ K + + H SN G E+E++G+M + G +F+VDA+Q
Sbjct: 119 AQPDEEGIISPEKIAQACTDQTKAIVLSHGSNVTGAYLELEKIGEMAEAHGIVFIVDASQ 178
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELC 253
+ G +++D+K+ + LA +GHKG +GP G GVL + D PL GGT EL
Sbjct: 179 TAGVIDIDMKRDRISMLACAGHKGLLGPQGTGVLIVAD---DYGLTPLSYGGTGSYSEL- 234
Query: 254 CYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELEIP 313
+ ++ P+R+E+GT N G GL AGI+Y+ G+ +I E L +R +GL ++
Sbjct: 235 IDQPSQWPDRYESGTLNTPGIAGLKAGIEYVLDNGVAQIFAHEWALTERLLKGLGAQKVD 294
Query: 314 WYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEH-KIMVRSGHHCALPVMKRLKI--NGTV 370
YGP K GVVSF + H+VA +LDEH +I VR+G HCA + L+ G +
Sbjct: 295 VYGPPLPRKRLGVVSFRLSGFDSHEVALILDEHYQIAVRAGLHCAPMTHETLQTTDTGLI 354
Query: 371 RASFHVYNSLEEVETFLGVLEELVKSLRS 399
R S +N+ EV+ L + ++ ++ S
Sbjct: 355 RVSVGPFNTEREVDQLLQAVMDIQQAFTS 383
>dbj|BAB07188.1| (AP001518) nitrogen fixation protein [Bacillus halodurans]
Length = 252
Score = 206 bits (519), Expect = 3e-52
Identities = 112/235 (47%), Positives = 154/235 (64%), Gaps = 10/235 (4%)
Query: 6 DVRKDIP-LTQEV-----IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMAT 59
+++K P L QEV +Y D+ ATS P VIEA+D+YY RY +NVHRGVH L +AT
Sbjct: 5 EIKKLFPVLDQEVNGSPLVYLDSAATSQKPIAVIEALDDYYRRYNSNVHRGVHTLGTLAT 64
Query: 60 QKYEESRKVVADFINAEF-DEIAFTKNTSESLNLVALGLEHL-FKKGDKIVTTPYEHHSN 117
YE +R+ + FI+A +EI FT+ T+ ++NLVA +GD+IV TP EHHSN
Sbjct: 65 DGYEGAREKIRRFIHASSTEEIIFTRGTTTAINLVAASYGRANLGEGDEIVITPMEHHSN 124
Query: 118 LLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEEL 176
++PWQ++AK G L ++ +G + + D EK I + K+VA+ HVSN LG I+ V+E+
Sbjct: 125 IIPWQQVAKATGATLTYLPLQKDGTIKIEDVEKTISEKTKIVAIMHVSNVLGTINPVKEI 184
Query: 177 GKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINK 231
++ GAI +VD AQS HM++DV++L DF AFSGHK GPTGIGVLY K
Sbjct: 185 AEIAHRHGAIMLVDGAQSAPHMKIDVQELGCDFFAFSGHK-MAGPTGIGVLYGKK 238
>pir||I64114 nifS protein homolog HI1295 - Haemophilus influenzae (strain Rd
KW20) >gi|1574753|gb|AAC22941.1| (U32809) nifS protein,
putative [Haemophilus influenzae Rd]
Length = 437
Score = 195 bits (490), Expect = 9e-49
Identities = 133/389 (34%), Positives = 204/389 (52%), Gaps = 17/389 (4%)
Query: 17 VIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAE 76
VIY DN AT+L P+ +I+ E+Y +VHR + +Q T +YE++R V ++++AE
Sbjct: 59 VIYLDNAATTLKPQVLIDRTAEFYAS-AGSVHRSQYDAAQ--TVQYEQARTQVKEWVHAE 115
Query: 77 FDE-IAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFI 135
+ +T T+ ++NLVA GL D+I+ + +HH+N + W AKK G K++ +
Sbjct: 116 DKHAVIWTSGTTHAINLVANGLMPQLNAEDEILISQADHHANFVTWHETAKKCGAKIQVL 175
Query: 136 EG-----DDEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEVEELGKMV-KEEGAIFVV 189
DE L A +EK KLVA+ VSN G ++ L +++ K A+ +V
Sbjct: 176 PILDNWLIDENALISALSEK----TKLVALNFVSNVTGTEQPIKRLIQLIRKHSNALVLV 231
Query: 190 DAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIED 249
DAAQ++ H+++D++ L ADFLAFS HK GP G+GVL +P GG ++
Sbjct: 232 DAAQAISHIKIDLQDLDADFLAFSAHK-IYGPNGLGVLTGKLTALSQLQPLFFGGKMVDR 290
Query: 250 VELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDE 309
V E P R EAGTPNI G IG A + +++K E+ L + L
Sbjct: 291 VSNDRITFAELPYRLEAGTPNIAGVIGFNAVLDWLQKWDFTAAEQYAISLAESVKVRLKS 350
Query: 310 LEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMKRLKINGT 369
E N + + VV F + D++++L E I +R G HCA P + RL T
Sbjct: 351 YENCRLF--NSPQASTVVCFVFDGIDCSDLSTLLSEQNIALRVGEHCAQPYLARLGERTT 408
Query: 370 VRASFHVYNSLEEVETFLGVLEELVKSLR 398
+R SF YN+ E+VE F L++ + L+
Sbjct: 409 LRLSFAPYNTQEDVEAFFTALDKALDLLQ 437
>sp|P75298|NISH_MYCPN NIFS-LIKE PROTEIN >gi|2146475|pir||S73681 nitrogen fixation protein
nifS - Mycoplasma pneumoniae (strain ATCC 29342)
>gi|1674033|gb|AAB96003.1| (AE000034) bifunctional;
nifS-like; also related to tRNA splicing protein spl 1;
molecular function: classV pyridoxal-phosphate-dependent
transferase; subunit to MPN487 [Mycoplasma pneumoniae]
Length = 408
Score = 172 bits (431), Expect = 7e-42
Identities = 128/388 (32%), Positives = 195/388 (49%), Gaps = 18/388 (4%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+ FDN ATS+ V +A EYY + N H L+ E+R++VAD+ N
Sbjct: 25 VNFDNAATSIALDTVSQACKEYYELFSVNPHNKTPDLNNQIIAIIAETRQLVADWFNVTA 84
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEG 137
EI FT + +ES+NL A GL+ K GD+IV EH +N+LPW LAK+ +L ++E
Sbjct: 85 LEIIFTSSATESINLFAHGLKPWIKPGDEIVLKGDEHSANVLPWVALAKQTKARLVWVE- 143
Query: 138 DDEGNLDLADAEKKI--KGAKLVAVQHVSNALGVIHEVEELGKMVKEEG--AIFVVDAAQ 193
+ N L D K + K+VA+ SN G + ++ +K+ A VDA Q
Sbjct: 144 -KQANQSLEDTFKSLINPKTKVVAITATSNLFGNSIDFAQIADYLKQVNPKAFVAVDAVQ 202
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVE-- 251
+V H ++D+ K + DFLAFS HK GPTG+GV YI K +P +GG ++
Sbjct: 203 TVQHKQIDIAKTQIDFLAFSTHKF-YGPTGLGVAYIKKSLQPQLQPLKLGGDIFTQIDPD 261
Query: 252 -LCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYI-EKIGIDKIEKQERKLVKRTTEGLDE 309
+K T P +FEAGTPNI L +++ +K ++ +L ++ E L+
Sbjct: 262 NTIHFKST--PLKFEAGTPNIMAIYALNKLLRFFKQKFNFAQMMAYGHQLKQQAYELLN- 318
Query: 310 LEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMKRLKINGT 369
P N D+ + SF L D+A+ L+ +KIMVR G C V + +
Sbjct: 319 -SNPQIVLANHDQDVPIFSFKHRQLATIDLATFLNINKIMVRQGSIC---VGRYRNKDYF 374
Query: 370 VRASFHVYNSLEEVETFLGVLEELVKSL 397
VR S YN+++E++ +L K++
Sbjct: 375 VRVSLMHYNTVKELQYLAKLLATDTKTI 402
>sp|Q49420|NISH_MYCGE NIFS-LIKE PROTEIN >gi|1361731|pir||B64237 nitrogen fixation protein
nifS homolog - Mycoplasma genitalium
>gi|3844918|gb|AAC71561.1| (U39715) nitrogen fixation
protein (nifS) [Mycoplasma genitalium]
Length = 408
Score = 170 bits (426), Expect = 3e-41
Identities = 126/388 (32%), Positives = 195/388 (49%), Gaps = 16/388 (4%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
I FDN ATS+ V EA EYY + N H ++Q EE+R ++A F NA+
Sbjct: 25 INFDNAATSIALDVVAEASKEYYQYFCVNPHNKNPEINQKLIAIIEETRDLLAKFFNAKK 84
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEG 137
+EI FT + +ESLNL A GL L K D+I+ EH +N+ PW LAK+ KL+ I+
Sbjct: 85 NEIIFTSSATESLNLFAFGLSSLVKSNDEIILKEDEHAANVFPWVNLAKENKAKLKIIKK 144
Query: 138 DDEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEVEELGKMVKE--EGAIFVVDAAQSV 195
+ A + KL+ + SN G + E++ K +K+ + VVDA Q+V
Sbjct: 145 TPNKSWTDAFLKACTPSTKLLVITATSNLFGNSIDYEKISKHLKKISPNSFIVVDAVQAV 204
Query: 196 GHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCY 255
H ++D+ DFL FS HK GPTG+G+ +I E +P +GG + ++ +
Sbjct: 205 PHHKIDITSANIDFLTFSTHKF-YGPTGLGIAFIKSELQSRLKPFKLGGDIFKSLD-NNF 262
Query: 256 KL--TEPPERFEAGTPNIGGAIGLAAGIKYIEK-IGIDKIEKQERKLVKRTTEGLDELEI 312
K+ E P +FEAGT NI L +K+++K ++ ++L + L +
Sbjct: 263 KIIFKEGPSKFEAGTLNIMAIYALNKQLKFMQKEFNFSEMVFYSKQLKNLAYQLLS--QN 320
Query: 313 PWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMKRLKINGTVRA 372
P N D+ + +F ++ D+A+ L+ KI+VR G C V K +R
Sbjct: 321 PNIVLANHDQDVPIFAFKHKYINSADLATFLNIKKIIVRQGSIC---VGKFKNKESFLRV 377
Query: 373 SFHVYNSLEEVETFLGVLEELVKSLRSS 400
S YN+ EE L LE+L+K+ ++S
Sbjct: 378 SLLHYNTKEE----LLYLEKLLKTSKNS 401
>pir||T35528 probable aminotransferase - Streptomyces coelicolor
>gi|4539215|emb|CAB39873.1| (AL049497) putative
aminotransferase [Streptomyces coelicolor A3(2)]
Length = 476
Score = 153 bits (383), Expect = 3e-36
Identities = 124/386 (32%), Positives = 179/386 (46%), Gaps = 39/386 (10%)
Query: 40 YLRYRANVHRGVHRLSQMATQKYEESRKVVADFINA-EFDEIAFTKNTSESLNLVALGLE 98
Y Y +VHRG LSQ++T +E +R VA F+ + D++ FT++T++SLNL+A L
Sbjct: 52 YAPYYGSVHRGAGYLSQLSTDLFENARGTVAGFLGCRDDDQVVFTRSTTDSLNLLAAALP 111
Query: 99 HLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKGAKLV 158
++ EHH++LLPW R A L + L+ A A ++ G LV
Sbjct: 112 ----AHCRVFVFETEHHASLLPW-RAADVTYLDAPRTPAEAVETLERALAAREPYGPALV 166
Query: 159 AVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGP 218
V SN G + V EL GA V+DAAQ H V V+ L D++AFSGHK
Sbjct: 167 CVTGASNVTGELWPVRELAAAAHAHGARIVLDAAQLAPHHPVSVRDLDVDWVAFSGHK-L 225
Query: 219 MGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCC-----YKLTEPPERFEAGTPNIGG 273
P G GVL ++ EP L GGG V + + R EAG+PN+ G
Sbjct: 226 YAPFGSGVLAGRADWLREAEPYLAGGGASRSVSRRADGGVDVQWHDSAARHEAGSPNVIG 285
Query: 274 AIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELEIPWYGPRNL----DKHAGVVSF 329
+A+ K + + G D + +E L+++ EGL E+P +L GV+SF
Sbjct: 286 VYAIASACKALTEAGFDTLVAREEYLIRKVREGL--AEVPEVRVLSLFGDDAPRVGVLSF 343
Query: 330 NVPPLH-PHDVASVLDEHKIMVRSGHHCALPVMKRL--------------------KING 368
V + H A++ E+ I VR G CA P+++ L K
Sbjct: 344 VVEGWNSSHFAAALSAEYGIGVRDGLFCAHPLVRTLLGSDPQTQGECGAPEAAPGEKSLN 403
Query: 369 TVRASFHVYNSLEEVETFLGVLEELV 394
+R SF E VE F+ + ELV
Sbjct: 404 AIRVSFGAGTPDEHVERFVSAVRELV 429
>emb|CAA05909.1| (AJ003152) cysteine desulphurase [Ruminococcus flavefaciens]
Length = 396
Score = 141 bits (352), Expect = 1e-32
Identities = 120/405 (29%), Positives = 196/405 (47%), Gaps = 42/405 (10%)
Query: 13 LTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADF 72
+ + IY DN AT+ + V+ AM Y+ N +++L + A + E +R+ VA
Sbjct: 1 MEKRFIYADNAATTAVSEEVLSAMLPYFRTAYGNAS-SIYKLGRDAQRDVELAREKVAKA 59
Query: 73 INAEFDEIAFTKNTSESLNLVALGLEHLF-KKGDK-IVTTPYEHHSNLLPWQRLAKKKGL 130
+ AE EI FT SES N G L KKG K IVT+ +EHH+ L + L +K G
Sbjct: 60 LGAEPREIYFTSCGSESDNWAIKGTAELMAKKGKKHIVTSVFEHHAVLHTCEYL-EKHGY 118
Query: 131 KLEFIEGDDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVV 189
++ ++ +D+G +D D K ++ LV + + +N +G I +EE+ + +E+G +F
Sbjct: 119 EVTYVPVNDKGLIDPEDVRKAVREDTALVTIMYANNEIGTIQPIEEIAAVCREKGVLFHT 178
Query: 190 DAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIED 249
DA Q+VGH+++DV K D L+ SGHK GIG +YI K + P L+ GG E
Sbjct: 179 DAVQAVGHVDIDVHKQGIDMLSLSGHK-IHAQKGIGAIYIRK---GIVLPNLVHGGGQER 234
Query: 250 VELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDE 309
+ AGT N+ +GL I + + ++ ++ R +DE
Sbjct: 235 GK-------------RAGTENVPAIVGLGVAI----EAAVRNTAEKAAIIIPRRNRIIDE 277
Query: 310 -LEIPWYGPRNLDKH---AGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCA-------- 357
L+IP Y N D+ G ++ + + + +LD + I SG C
Sbjct: 278 LLKIP-YTRLNGDREKRLPGNINISFEGIEGESLLLMLDLNGICASSGSACTSGSLDPSH 336
Query: 358 --LPV-MKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
L + +K +G++R S S E+VE + + ++V+ LRS
Sbjct: 337 VLLSIGLKHAVAHGSLRLSIEEDVSDEDVEYIIETIPKVVQRLRS 381
>gb|AAG01802.1|AF276772_1 (AF276772) cysteine desulfurase NifS [Methanosarcina thermophila]
Length = 404
Score = 138 bits (343), Expect = 2e-31
Identities = 114/408 (27%), Positives = 190/408 (45%), Gaps = 41/408 (10%)
Query: 9 KDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKV 68
+++ + +Y DN+AT+ K V+EAM Y N ++ + + + +RK
Sbjct: 9 ENVSTENKAVYMDNSATTPVRKEVVEAMLPYMTENFGNPS-SIYEIGKTSKHAINLARKK 67
Query: 69 VADFINAEFDEIAFTKNTSESLNLVALGLEHLFK-KGDKIVTTPYEHHSNLLPWQRLAKK 127
AD + AE +EI FT +ES N G+ + KG I+T+ EHH+ L L +
Sbjct: 68 AADALGAEENEIYFTSGGTESDNWAIKGIAFANRDKGKHIITSSIEHHAVLHTCAWL-EG 126
Query: 128 KGLKLEFIEGDDEGNLDLADAEKKIKGAK-LVAVQHVSNALGVIHEVEELGKMVKEEGAI 186
+G ++ ++ D G + + I+ L+++ +N +G I ++E+G++ KE
Sbjct: 127 QGFEVTYLPVDKYGMVSPDELRNAIRDDTILISIMFANNEIGTIQPIKEIGEIAKENQIY 186
Query: 187 FVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGT 246
F DA Q++GH+ +DVKKL D L+ SGH+ GP G G LYI K V PL+ GG
Sbjct: 187 FHTDAVQAIGHVPIDVKKLNIDLLSLSGHEFE-GPKGCGALYIRK---GVKIDPLLHGGA 242
Query: 247 IEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVK---RT 303
E + AGT N+ G +GL ++ +IE+ R L+K R
Sbjct: 243 QE-------------RKRRAGTENVPGIVGLGKA----TELATAEIEESNRTLLKLRDRL 285
Query: 304 TEGLDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHC------- 356
EGL ++ + A V+ + + +L+ I +G C
Sbjct: 286 IEGLLKIPKTHLNGHPTQRLANNVNVTFEYIEGESLLLLLNAKGIYASTGSACNSSSLEP 345
Query: 357 -----ALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
A V + I+G++R S N+ E+V+ L V+ E+V+ LR+
Sbjct: 346 SHVLTACGVPHEI-IHGSLRLSLGRMNTSEDVDRVLEVVPEIVQKLRN 392
>pir||H70364 iron-sulfur cofactor synthesis protein nifS - Aquifex aeolicus
>gi|2983322|gb|AAC06912.1| (AE000705) FeS cluster
formation protein NifS [Aquifex aeolicus]
Length = 406
Score = 137 bits (342), Expect = 2e-31
Identities = 119/403 (29%), Positives = 190/403 (46%), Gaps = 41/403 (10%)
Query: 15 QEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFIN 74
++V+Y D+ AT+ + V+EAM Y+ R + +H Q A + E++R+ VA IN
Sbjct: 8 KKVVYVDHIATTPVAEEVLEAMLPYF-REKFGNPTSLHSFGQEAKKAVEKAREQVAQLIN 66
Query: 75 AEF-DEIAFTKNTSESLNLVALGLEHLF-KKGDKIVTTPYEHHSNLLPWQRLAKKKGLKL 132
A +EI FT E+ NL G+ + ++G IVTT EHHS L P + L +++G ++
Sbjct: 67 ANIPEEIIFTSGGIEANNLAIKGIAKAYQRRGKHIVTTEIEHHSILHPCKTL-EREGWEV 125
Query: 133 EFIEGDDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEG--AIFVV 189
+++ D G +D + ++ LV++ H + +G I ++EL K KE+ IF
Sbjct: 126 TYLKPDKYGLIDPEQVREAVREDTVLVSIGHSNREIGTIQNIKELVKAAKEKNPKVIFHT 185
Query: 190 DAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIED 249
DAA S+GH VDV+ D +F+ H GP G+G L+ K V PLI GGT E
Sbjct: 186 DAAPSLGHYPVDVQDWGVDAASFTAHL-MYGPKGVGALWTRK---GVKVKPLIEGGTQE- 240
Query: 250 VELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQER---KLVKRTTEG 306
AGT N+ G +G A + K D++++ KL K E
Sbjct: 241 ------------RGVRAGTENVPGIVGFGAAAELAMKELDDRMKRLSHYRDKLRKGLEEK 288
Query: 307 LDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMKRLKI 366
+D +E + + L H ++ V + + LD I SG C +K+ +
Sbjct: 289 VDYIEFTGHPTQRLPHHLSII---VHFVEGEAMLLRLDLMGIETASGSACVSLALKQSHV 345
Query: 367 -----------NGTVRASFHVYNSLEEVETFLGVLEELVKSLR 398
NG+V SF N+ E+V+ L +++ LR
Sbjct: 346 LTAIGIPKEVSNGSVVFSFGRENTEEDVDYILEEFPKVINWLR 388
>sp|P25374|SPL1_YEAST TRNA SPLICING PROTEIN SPL1 (NIFS-LIKE 54.5 KD PROTEIN)
>gi|83348|pir||S19343 probable iron-sulfur cofactor
synthesis protein NIFS1 - yeast (Saccharomyces
cerevisiae) >gi|172038|gb|AAA34814.1| (M98808) nitrogen
fixation-like protein [Saccharomyces cerevisiae]
>gi|1907129|emb|CAA42344.1| (X59720) YCL017c, len:497
[Saccharomyces cerevisiae]
Length = 497
Score = 136 bits (340), Expect = 3e-31
Identities = 80/229 (34%), Positives = 119/229 (51%), Gaps = 2/229 (0%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
IY D AT+ T V++ M ++Y N H H E +R VA INA+
Sbjct: 100 IYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETNTAVENARAYVAKMINADP 159
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEG 137
EI FT +ES N+V G+ +KK K + T H +L R K+G ++ F+
Sbjct: 160 KEIIFTSGATESNNMVLKGVPRFYKKTKKHIITTRTEHKCVLEAARAMMKEGFEVTFLNV 219
Query: 138 DDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVG 196
DD+G +DL + E I+ LV+V V+N +GVI ++E+G + ++ F DAAQ+ G
Sbjct: 220 DDQGLIDLKELEDAIRPDTCLVSVMAVNNEIGVIQPIKEIGAICRKNKIYFHTDAAQAYG 279
Query: 197 HMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
+ +DV ++ D L+ S HK GP GIG +Y+ + EP L GGG
Sbjct: 280 KIHIDVNEMNIDLLSISSHK-IYGPKGIGAIYVRRRPRVRLEPLLSGGG 327
>gb|AAK42385.1| NifS protein homolog (nifS) [Sulfolobus solfataricus]
Length = 376
Score = 136 bits (338), Expect = 6e-31
Identities = 113/406 (27%), Positives = 193/406 (46%), Gaps = 50/406 (12%)
Query: 1 MRIPEDVRKDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQ 60
+R P + R+++P+T++ +Y ++ + S TP P + + Y Y +RG +++
Sbjct: 3 LRDPREFRENVPVTRKYVYLNHASVSPTPLPSL--FEAYRYLYEV-ANRGSIAVNEEEED 59
Query: 61 KYEESRKVVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLP 120
+ R +++ + A DEI+ NTS +NLVA GLE +K D IVT E + + P
Sbjct: 60 ELYHIRSKISNLVGAYSDEISLIPNTSYGVNLVAHGLE--WKGDDNIVTDNLEFPTVVYP 117
Query: 121 WQRLAKKKGLKLEFIEGD----DEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEVEEL 176
+ +L KK G+K+ +E + +E + D K +LVA+ HVS G+ +V ++
Sbjct: 118 FLKLTKK-GVKINIVETNPYTFEEDIISHID-----KNTRLVAISHVSFNTGLKVDVRKI 171
Query: 177 GKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDV 236
K +E + ++D QS G ++++VK+L DF G+K M P G G +Y+ + +
Sbjct: 172 VKAARENNTLVLLDIIQSAGAVKINVKELGIDFAIAGGYKWLMSPQGSGFIYVKRGLIE- 230
Query: 237 FEPPLIGGGTIE---DVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIE 293
+PP G T D + L + P RFE GT ++ + LA K E IG
Sbjct: 231 -DPPFYGWKTSADYLDFNPNKFTLEKGPRRFEIGTVDLAANLSLA---KSCEIIG----- 281
Query: 294 KQERKLVKRTTEGLDELEIPWYGPRNL------DKHAGVVSFNVPPLHPHDVASVLDEHK 347
+ +L++ + L + I ++ DK AG+V V P ++A L +
Sbjct: 282 -ENMELIESSVTNLSQFAIRLAKDHSMEVITPEDKRAGIVIVKVK--KPKEIAKELLKEN 338
Query: 348 IMVRSGHHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEEL 393
I+V +R S H YN+ EEV+ + + EL
Sbjct: 339 IVVSPR-------------GEGIRISTHFYNTEEEVQKTIEKISEL 371
>gi|10383773 NifS-like protein; Nfs1p [Saccharomyces cerevisiae]
Length = 497
Score = 136 bits (338), Expect = 6e-31
Identities = 80/229 (34%), Positives = 119/229 (51%), Gaps = 2/229 (0%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
IY D AT+ T V++ M ++Y N H H E +R VA INA+
Sbjct: 100 IYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETNTAVENARAHVAKMINADP 159
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEG 137
EI FT +ES N+V G+ +KK K + T H +L R K+G ++ F+
Sbjct: 160 KEIIFTSGATESNNMVLKGVPRFYKKTKKHIITTRTEHKCVLEAARAMMKEGFEVTFLNV 219
Query: 138 DDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVG 196
DD+G +DL + E I+ LV+V V+N +GVI ++E+G + ++ F DAAQ+ G
Sbjct: 220 DDQGLIDLKELEDAIRPDTCLVSVMAVNNEIGVIQPIKEIGAICRKNKIYFHTDAAQAYG 279
Query: 197 HMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
+ +DV ++ D L+ S HK GP GIG +Y+ + EP L GGG
Sbjct: 280 KIHIDVNEMNIDLLSISSHK-IYGPKGIGAIYVRRRPRVRLEPLLSGGG 327
>pir||F72223 iron-sulfur cofactor synthesis protein TM1692 [similarity] -
Thermotoga maritima (strain MSB8)
>gi|4982268|gb|AAD36759.1|AE001809_11 (AE001809)
aminotransferase, class V [Thermotoga maritima]
Length = 384
Score = 135 bits (336), Expect = 1e-30
Identities = 112/396 (28%), Positives = 187/396 (46%), Gaps = 38/396 (9%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+YFDN AT+ V+E M +Y N + H + A E++R+ VA +
Sbjct: 3 VYFDNNATTRVDDRVLEEMIVFYREKYGNPN-SAHGMGIEANLHMEKAREKVAKVLGVSP 61
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDK-IVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
EI FT +ES+N + + F+K + I+TTP EH + +L + KG K++++
Sbjct: 62 SEIFFTSCATESINWILKTVAETFEKRKRTIITTPIEHKA-VLETMKYLSMKGFKVKYVP 120
Query: 137 GDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEELGKMVKEEG--AIFVVDAAQ 193
D G + L + EK + + LV++ +N +G I VE++ ++VK++ + VDA Q
Sbjct: 121 VDSRGVVKLEELEKLVDEDTFLVSIMAANNEVGTIQPVEDVTRIVKKKNKETLVHVDAVQ 180
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELC 253
++G + ++KL+ D+ +FS HK GP G+G+ YI K V PLI GG E
Sbjct: 181 TIGKIPFSLEKLEVDYASFSAHKF-HGPKGVGITYIRK---GVPIRPLIHGGGQE----- 231
Query: 254 CYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELEIP 313
+GT N+ G +G A ++ I + + K KL + GL L
Sbjct: 232 --------RGLRSGTQNVPGIVGAARAME-IAVEELSEAAKHMEKLRSKLVSGLMNLGAH 282
Query: 314 WYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHC------------ALPVM 361
P + +S + P + + ++L + I V + C A+ V
Sbjct: 283 IITPLEISL-PNTLSVSFPNIRGSTLQNLLSGYGIYVSTSSACTSKDERLSHVLDAMGVD 341
Query: 362 KRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSL 397
+R+ G +R S YN+ EEV+ FL +EE++ L
Sbjct: 342 RRI-AQGAIRISLCKYNTEEEVDYFLKKIEEILSFL 376
>pdb|1ECX|B Chain B, Nifs-Like Protein >gi|7546479|pdb|1ECX|A Chain A,
Nifs-Like Protein
Length = 384
Score = 135 bits (336), Expect = 1e-30
Identities = 112/396 (28%), Positives = 187/396 (46%), Gaps = 38/396 (9%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+YFDN AT+ V+E M +Y N + H + A E++R+ VA +
Sbjct: 3 VYFDNNATTRVDDRVLEEMIVFYREKYGNPN-SAHGMGIEANLHMEKAREKVAKVLGVSP 61
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDK-IVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
EI FT +ES+N + + F+K + I+TTP EH + +L + KG K++++
Sbjct: 62 SEIFFTSCATESINWILKTVAETFEKRKRTIITTPIEHKA-VLETMKYLSMKGFKVKYVP 120
Query: 137 GDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEELGKMVKEEG--AIFVVDAAQ 193
D G + L + EK + + LV++ +N +G I VE++ ++VK++ + VDA Q
Sbjct: 121 VDSRGVVKLEELEKLVDEDTFLVSIMAANNEVGTIQPVEDVTRIVKKKNKETLVHVDAVQ 180
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELC 253
++G + ++KL+ D+ +FS HK GP G+G+ YI K V PLI GG E
Sbjct: 181 TIGKIPFSLEKLEVDYASFSAHKF-HGPKGVGITYIRK---GVPIRPLIHGGGQE----- 231
Query: 254 CYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELEIP 313
+GT N+ G +G A ++ I + + K KL + GL L
Sbjct: 232 --------RGLRSGTQNVPGIVGAARAME-IAVEELSEAAKHMEKLRSKLVSGLMNLGAH 282
Query: 314 WYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHC------------ALPVM 361
P + +S + P + + ++L + I V + C A+ V
Sbjct: 283 IITPLEISL-PNTLSVSFPNIRGSTLQNLLSGYGIYVSTSSACTSKDERLRHVLDAMGVD 341
Query: 362 KRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSL 397
+R+ G +R S YN+ EEV+ FL +EE++ L
Sbjct: 342 RRI-AQGAIRISLCKYNTEEEVDYFLKKIEEILSFL 376
>emb|CAA07007.1| (AJ006406) AgSPL1 [Eremothecium gossypii]
Length = 490
Score = 132 bits (330), Expect = 5e-30
Identities = 77/229 (33%), Positives = 120/229 (51%), Gaps = 2/229 (0%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
IY D AT+ T V++ M ++Y N H H +Q+ E++RK VAD I A+
Sbjct: 93 IYLDMQATTPTDPRVVDTMLKFYTGLYGNPHSNTHSYGWETSQEVEKARKNVADVIKADP 152
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEG 137
EI FT +ES N+ G+ +KK + T H +L R K +G + F+
Sbjct: 153 KEIIFTSGATESNNMALKGVARFYKKRKNHIITTRTEHKCVLEAARSMKDEGFDVTFLNV 212
Query: 138 DDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVG 196
+++G + L + E+ I+ LV+V V+N +GV+ ++E+G + + F DAAQ+ G
Sbjct: 213 NEDGLVSLEELEQAIRPETSLVSVMSVNNEIGVVQPIKEIGAICRRHKVFFHSDAAQAYG 272
Query: 197 HMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
+ +DV ++ D L+ S HK GP GIG LY+ + EP L GGG
Sbjct: 273 KIPIDVDEMNIDLLSISSHK-IYGPKGIGALYVRRRPRVRMEPLLSGGG 320
>sp|P87185|SPL1_CANAL TRNA SPLICING PROTEIN SPL1 >gi|2121284|gb|AAC49940.1| (AF000120)
tRNA splicing protein [Candida albicans]
Length = 488
Score = 131 bits (326), Expect = 1e-29
Identities = 75/229 (32%), Positives = 120/229 (51%), Gaps = 2/229 (0%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
IY D AT+ V++ M E+Y N H H ++ E++R +AD INA+
Sbjct: 89 IYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINADP 148
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEG 137
EI FT +E+ N+ G+ +KK K + T H +L R + +G ++ ++
Sbjct: 149 KEIIFTSGATETNNMAIKGVPRFYKKTKKHIITTQTEHKCVLDSARHMQDEGFEVTYLPV 208
Query: 138 DDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVG 196
EG ++L D +K I K LV++ V+N +GVI ++E+GK+ +E F DAAQ+ G
Sbjct: 209 SSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQAYG 268
Query: 197 HMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
+ +DV ++ D L+ S HK GP GIG Y+ + +P + GGG
Sbjct: 269 KIPIDVNEMNIDLLSISSHK-IYGPKGIGACYVRRRPRVRLDPIITGGG 316
>pdb|1EG5|B Chain B, Nifs-Like Protein >gi|7546280|pdb|1EG5|A Chain A,
Nifs-Like Protein
Length = 384
Score = 130 bits (323), Expect = 3e-29
Identities = 112/396 (28%), Positives = 183/396 (45%), Gaps = 38/396 (9%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+YFDN AT+ V+E +Y N + H A E++R+ VA +
Sbjct: 3 VYFDNNATTRVDDRVLEEXIVFYREKYGNPN-SAHGXGIEANLHXEKAREKVAKVLGVSP 61
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDK-IVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
EI FT +ES+N + + F+K + I+TTP EH + +L + KG K++++
Sbjct: 62 SEIFFTSCATESINWILKTVAETFEKRKRTIITTPIEHKA-VLETXKYLSXKGFKVKYVP 120
Query: 137 GDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEELGKMVKEEG--AIFVVDAAQ 193
D G + L + EK + + LV++ +N +G I VE++ ++VK++ + VDA Q
Sbjct: 121 VDSRGVVKLEELEKLVDEDTFLVSIXAANNEVGTIQPVEDVTRIVKKKNKETLVHVDAVQ 180
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELC 253
++G + ++KL+ D+ +FS HK GP G+G+ YI K V PLI GG E
Sbjct: 181 TIGKIPFSLEKLEVDYASFSAHKF-HGPKGVGITYIRK---GVPIRPLIHGGGQE----- 231
Query: 254 CYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELEIP 313
+GT N+ G +G AA I + + K KL + GL L
Sbjct: 232 --------RGLRSGTQNVPGIVG-AARAXEIAVEELSEAAKHXEKLRSKLVSGLXNLGAH 282
Query: 314 WYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHC------------ALPVM 361
P + +S + P + + ++L + I V + C A V
Sbjct: 283 IITPLEISL-PNTLSVSFPNIRGSTLQNLLSGYGIYVSTSSACTSKDERLRHVLDAXGVD 341
Query: 362 KRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSL 397
+R+ G +R S YN+ EEV+ FL +EE++ L
Sbjct: 342 RRI-AQGAIRISLCKYNTEEEVDYFLKKIEEILSFL 376
>sp|P87187|SPL1_CANMA TRNA SPLICING PROTEIN SPL1 >gi|2121288|gb|AAC49935.1| (AF000115)
tRNA splicing protein [Candida maltosa]
Length = 484
Score = 129 bits (321), Expect = 6e-29
Identities = 75/229 (32%), Positives = 120/229 (51%), Gaps = 2/229 (0%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
IY D AT+ V++ M E+Y N H H ++ E++R +AD INA+
Sbjct: 85 IYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARGYIADVINADP 144
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEG 137
EI FT +E+ N+ G+ +KK K + T H +L R + +G + ++
Sbjct: 145 KEIIFTSGATETNNMAIKGVPRFYKKTKKHIITTQTEHKCVLDSARHMQDEGFDITYLPV 204
Query: 138 DDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVG 196
+ EG ++L D +K I K LV+V V+N +GVI ++E+G++ +E F DAAQ+ G
Sbjct: 205 NSEGLINLDDLKKAIRKDTVLVSVMAVNNEIGVIQPLKEIGQICRENKIFFHTDAAQAYG 264
Query: 197 HMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
+ +DV ++ D L+ S HK GP GIG Y+ + +P + GGG
Sbjct: 265 KIPIDVNEMNIDLLSISSHK-IYGPKGIGACYVRRRPRVRLDPIITGGG 312
>pir||JC4557 capreomycin acetyltransferase (EC 2.3.1.-) - Streptomyces capreolus
>gi|1223838|gb|AAA92035.1| (U13077) capreomycin
acetyltransferase [Streptomyces capreolus]
>gi|1586532|prf||2204233A capreomycin acetyltransferase
[Streptomyces capreolus]
Length = 359
Score = 128 bits (319), Expect = 1e-28
Identities = 101/365 (27%), Positives = 169/365 (45%), Gaps = 15/365 (4%)
Query: 36 MDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEFDEIAFTKNTSES----LN 91
+DE R G + ++ E V A + A DE+AFT + + S +
Sbjct: 2 LDEVVGHLRREAEIGGYVAARERADDLEAGYGVFASLMGAHPDEVAFTDSATRSWLAAFD 61
Query: 92 LVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKK 151
V LG GD+++ T E+ N +P A++ G ++ I D G +D+ +
Sbjct: 62 AVPLG------PGDRVLVTEVEYGGNAIPILHRAREVGFEVVTIPSDAVGQVDVEALREL 115
Query: 152 I-KGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFL 210
+ + KLV++ H G+++ V E+ E GA+ ++DA QS+G + V +L D +
Sbjct: 116 VDERVKLVSLVHAPTNGGLVNPVREVASAAHEVGALVLLDACQSLGQLPVRADELGVDII 175
Query: 211 AFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPN 270
+ +G K GP G GVL + + D P L+ + E V KL E +E +
Sbjct: 176 SATGRKWLRGPRGTGVLVVCRSAADRLRPRLVDLHSGEWVGPEEVKLREDARVYELWETS 235
Query: 271 IGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELEIPWYGPRNLD-KHAGVVSF 329
+ +GL A +Y +GID I + R +R GL E+P R++ + +G+V+F
Sbjct: 236 VADRLGLIAAARYALDLGIDAIAAEVRSRAERLRAGL--REVPGVEVRDIGARLSGIVTF 293
Query: 330 NVPPLHPHDVASVLDEHKIMVRSGHHCALPV-MKRLKINGTVRASFHVYNSLEEVETFLG 388
V P V L H ++V + H + + M R + G VR S H + S E+V+ +
Sbjct: 294 TVEGRDPVAVRDELARHDVVVTTSHLTSTRIDMTRRGVEGLVRMSPHYFVSPEQVDRAVE 353
Query: 389 VLEEL 393
V+ L
Sbjct: 354 VVAAL 358
>gi|11497803 nifS protein, class-V aminotransferase (nifS-1) [Archaeoglobus
fulgidus] >gi|7447239|pir||B69273 iron-sulfur cofactor
synthesis protein nifS-1 - Archaeoglobus fulgidus
>gi|2650451|gb|AAB91039.1| (AE001093) nifS protein,
class-V aminotransferase (nifS-1) [Archaeoglobus
fulgidus]
Length = 392
Score = 128 bits (319), Expect = 1e-28
Identities = 106/397 (26%), Positives = 186/397 (46%), Gaps = 34/397 (8%)
Query: 16 EVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINA 75
E+ YFD +T + V+EAM Y + N VH A + EE+R +++ IN
Sbjct: 10 EMPYFDYASTKPVDERVVEAMLPYMTEHFGNPS-SVHSYGFKAREVIEEARGKISELING 68
Query: 76 EFDEIAFTKNTSESLNLVALGLE-HLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEF 134
+I FT +ES NL +G +KG ++ + EH S + P + L +K+G ++EF
Sbjct: 69 GGGDIVFTSGATESNNLALIGYAMRNARKGKHVLVSSIEHMSVINPAKYL-QKQGFEVEF 127
Query: 135 IEGDDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQ 193
I D G +DL E+KI+ LV+VQH +N +G I ++E+ +++ A+ VDA
Sbjct: 128 IPVDKYGTVDLEFIEEKIREDTILVSVQHANNEIGTIQPIKEISEIINGRAALH-VDATA 186
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELC 253
S+G +EVDV+K+ AD L S + GP G+G L++ + +P ++GGG
Sbjct: 187 SLGQIEVDVEKIGADMLTISSN-DIYGPKGVGALWVRQGV--RLQPIILGGG-------- 235
Query: 254 CYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELEIP 313
+ +G+ N+ +G + K ++ + R+L R + + ++E
Sbjct: 236 ------QEKGLRSGSENVPAIVGFGKAAEITAKEWGEEAGRL-RRLRDRIIDNVLKIEES 288
Query: 314 WYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPV-----------MK 362
+ + V+ + + LD I +G C+ +K
Sbjct: 289 YLNGHPEKRLPNNVNVRFSYIEGESIVLSLDMAGIQASTGSACSSKTLQPSHVLMACGLK 348
Query: 363 RLKINGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
+ +GT+ + YN+ E+V+ L VL +++ LRS
Sbjct: 349 HEEAHGTLLLTLGRYNTDEDVDRLLEVLPGVIERLRS 385
>pir||G82285 aminotransferase NifS, class V VC0748 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9655193|gb|AAF93913.1|
(AE004160) aminotransferase NifS, class V [Vibrio
cholerae]
Length = 404
Score = 128 bits (319), Expect = 1e-28
Identities = 102/396 (25%), Positives = 181/396 (44%), Gaps = 32/396 (8%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRA--NVHRGVHRLSQMATQKYEESRKVVADFINA 75
IY D +AT V E M +Y N HR A + + +R+ +A +NA
Sbjct: 5 IYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAALLNA 64
Query: 76 EFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFI 135
+ EI FT +ES NL G+ H + K K + T H +L R +++G ++ ++
Sbjct: 65 DPREIVFTSGATESDNLAIKGVAHFYNKQGKHIITSKTEHKAVLDTMRQLEREGFEVTYL 124
Query: 136 EGDDEGNLDLADAEKKIKGAK-LVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQS 194
+ + G +DLA E ++ LV++ HV+N +GV+ ++ +G++ + +F VDAAQS
Sbjct: 125 DPESNGLVDLAKLEAAMRDDTILVSIMHVNNEIGVVQDIAAIGELCRSRKVVFHVDAAQS 184
Query: 195 VGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCC 254
G + +DV+++K D ++ S HK GP GIG LY+ ++ E + GGG
Sbjct: 185 AGKVAIDVQEMKVDLISLSAHKA-YGPKGIGALYVRRKPRIRLEAQMHGGG--------- 234
Query: 255 YKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELEIPW 314
F +GT +G+ + I K + + KL R +G+ ++E
Sbjct: 235 -----HERGFRSGTLPTHQIVGMGEAFR-IAKEELQQDYDHALKLRNRLLDGIKDMEAVT 288
Query: 315 YGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALP------VMKRLKIN- 367
+LD+ + NV + ++ + V SG C V++ L +N
Sbjct: 289 IN-GDLDQRV-PHNLNVSFAFVEGESLLMALKDLAVSSGSACTSASLEPSYVLRALGLND 346
Query: 368 ----GTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
++R SF + + E++ + ++ V LR+
Sbjct: 347 ELAHSSIRFSFGRFTTEAEIDYAIELIRVAVDKLRA 382
>pir||G83168 L-cysteine desulfurase (pyridoxal phosphate-dependent) PA3814
[imported] - Pseudomonas aeruginosa (strain PAO1)
>gi|9949988|gb|AAG07201.1|AE004799_7 (AE004799)
L-cysteine desulfurase (pyridoxal phosphate-dependent)
[Pseudomonas aeruginosa]
Length = 404
Score = 127 bits (316), Expect = 2e-28
Identities = 78/232 (33%), Positives = 128/232 (54%), Gaps = 6/232 (2%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYR--ANVHRGVHRLSQMATQKYEESRKVVADFINA 75
IY D +AT+ V + M E L N H A + E +R+ VA+ +NA
Sbjct: 5 IYLDYSATTPVDPRVAQKMSECLLMDGNFGNPASRSHVFGWKAEEAVENARRQVAELVNA 64
Query: 76 EFDEIAFTKNTSESLNLVALGLEHLFK-KGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEF 134
+ EI +T +ES NL G+ H + KG I+T+ EH + +L R +++G ++ +
Sbjct: 65 DPREIVWTSGATESDNLAIKGVAHFYSGKGKHIITSKIEHKA-VLDTCRQLEREGFEVTY 123
Query: 135 IEGDDEGNLDLADAEKKIKGAK-LVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQ 193
+E ++G + A E ++ LV+V HV+N +G ++++ +G++ + G +F VDAAQ
Sbjct: 124 LEPGEDGLITPALVEAALRDDTILVSVMHVNNEIGTVNDIAAIGELTRSRGVLFHVDAAQ 183
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
S G +E+D+ KLK D ++FS HK GP GIG LY+ ++ E + GGG
Sbjct: 184 STGKVEIDLDKLKVDLMSFSAHK-TYGPKGIGALYVRRKPRVRIEGQMHGGG 234
>gb|AAF62329.1| (AE002486) nifS protein [Neisseria meningitidis MC58]
Length = 404
Score = 126 bits (313), Expect = 5e-28
Identities = 79/230 (34%), Positives = 122/230 (52%), Gaps = 4/230 (1%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+Y D AT K V E M Y N H A + E++R +A INA+
Sbjct: 7 VYLDYAATPPVDKRVAEKMIPYLTETFGNPASNSHSFGWEAEEAVEKARADIAALINADS 66
Query: 78 DEIAFTKNTSESLNLVALGLEHLFK-KGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
EI FT +ES NL G H +K KG+ ++T EH + +L R +++G ++ +++
Sbjct: 67 KEIVFTSGATESNNLAIKGAAHFYKSKGNHLITVKTEHKA-VLDTMRELERQGYEVTYLD 125
Query: 137 GDDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSV 195
+ G +DL + I+ LV+V V+N +GV+ ++ +G++ +E IF VDAAQ+
Sbjct: 126 VQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVVQDIPAIGEICRERKIIFHVDAAQAC 185
Query: 196 GHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
G + VDV+ K D L+ SGHK GP GIG LY+ ++ E + GGG
Sbjct: 186 GKVPVDVEAAKVDLLSMSGHK-VYGPKGIGALYVRRKPRVRLEAQMHGGG 234
>pir||A65030 probable iron-sulfur cofactor synthesis protein b2530 - Escherichia
coli (strain K-12) >gi|1788879|gb|AAC75583.1| (AE000339)
putative aminotransferase [Escherichia coli K12]
Length = 412
Score = 125 bits (312), Expect = 7e-28
Identities = 74/231 (32%), Positives = 122/231 (52%), Gaps = 4/231 (1%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRA--NVHRGVHRLSQMATQKYEESRKVVADFINA 75
IY D +AT+ V E M ++ N HR A + + +R +AD + A
Sbjct: 13 IYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFGWQAEEAVDIARNQIADLVGA 72
Query: 76 EFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFI 135
+ EI FT +ES NL G + ++K K + T H +L R +++G ++ ++
Sbjct: 73 DPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYL 132
Query: 136 EGDDEGNLDLADAEKKIKGAK-LVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQS 194
G +DL + E ++ LV++ HV+N +GV+ ++ +G+M + G I+ VDA QS
Sbjct: 133 APQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVVQDIAAIGEMCRARGIIYHVDATQS 192
Query: 195 VGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
VG + +D+ +LK D ++FSGHK GP GIG LY+ ++ E + GGG
Sbjct: 193 VGKLPIDLSQLKVDLMSFSGHK-IYGPKGIGALYVRRKPRVRIEAQMHGGG 242
>sp|P39171|NIFS_ECOLI NIFS PROTEIN HOMOLOG >gi|1799938|dbj|BAA16424.1| (D90883) UNKNOWN
PROTEIN FROM 2D-PAGE (SPOT M92) (FRAGMENT). [Escherichia
coli]
Length = 404
Score = 125 bits (312), Expect = 7e-28
Identities = 74/231 (32%), Positives = 122/231 (52%), Gaps = 4/231 (1%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRA--NVHRGVHRLSQMATQKYEESRKVVADFINA 75
IY D +AT+ V E M ++ N HR A + + +R +AD + A
Sbjct: 5 IYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFGWQAEEAVDIARNQIADLVGA 64
Query: 76 EFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFI 135
+ EI FT +ES NL G + ++K K + T H +L R +++G ++ ++
Sbjct: 65 DPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYL 124
Query: 136 EGDDEGNLDLADAEKKIKGAK-LVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQS 194
G +DL + E ++ LV++ HV+N +GV+ ++ +G+M + G I+ VDA QS
Sbjct: 125 APQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVVQDIAAIGEMCRARGIIYHVDATQS 184
Query: 195 VGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
VG + +D+ +LK D ++FSGHK GP GIG LY+ ++ E + GGG
Sbjct: 185 VGKLPIDLSQLKVDLMSFSGHK-IYGPKGIGALYVRRKPRVRIEAQMHGGG 234
>pir||A83275 hypothetical protein PA2958 [imported] - Pseudomonas aeruginosa
(strain PAO1) >gi|9949056|gb|AAG06346.1|AE004722_2
(AE004722) hypothetical protein [Pseudomonas aeruginosa]
Length = 377
Score = 125 bits (312), Expect = 7e-28
Identities = 99/386 (25%), Positives = 187/386 (47%), Gaps = 28/386 (7%)
Query: 14 TQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFI 73
T + Y ++ A + P+ +A+ + R NV G + ++ R+ +A +
Sbjct: 11 TNGLRYLNHAAVAPWPRRAADAVSAFA---RENVAHGASDYPEWLKIEHR-LRERLARLL 66
Query: 74 NAEF-DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKL 132
NA ++A KNTSE+L+ VA GL+ +++GD++V E SN + W+ L + +
Sbjct: 67 NARAGSDLALVKNTSEALSFVAFGLD--WREGDQVVIGNEEFPSNRVVWEALRPRGVEVV 124
Query: 133 EF-IEGDDEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDA 191
E + GDD + LA + + + AVQ+ S G+ ++E +G + G +F +DA
Sbjct: 125 EVGLAGDDPESALLAACGPRTRLLSISAVQYAS---GLRLDLERIGAGCRRRGVLFCIDA 181
Query: 192 AQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIE--- 248
Q +G + DV+ F G+K +GP G+GV Y + E + G +E
Sbjct: 182 IQQLGALPFDVQAYDCAFAMADGYKWMLGPEGLGVFYCHPEQRERLALHEYGWHMLEHAL 241
Query: 249 DVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLD 308
D E ++ RFE G+PN+ GA+ L A + +E++G++++ + + V+ +GL
Sbjct: 242 DYERRDWRPARSARRFECGSPNMLGAMALEASLGLLEEVGMERVGRDIAERVQWLQDGLS 301
Query: 309 ELE-IPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMKRLKIN 367
+ + + PRN + AG+++F++ + L + +++ CA +
Sbjct: 302 GIPGVRLHSPRNPSRRAGILTFSLDGQDNRTLFETLRQERVI------CA-------QRG 348
Query: 368 GTVRASFHVYNSLEEVETFLGVLEEL 393
G +R S H Y S ++ L +L ++
Sbjct: 349 GGIRFSPHFYTSSTVIDDTLAILRQV 374
>sp|Q43884|NIFS_ANAAZ NIFS PROTEIN >gi|762778|gb|AAA87249.1| (L34879) NifS gene product
[Anabaena azollae]
Length = 400
Score = 125 bits (310), Expect = 1e-27
Identities = 109/399 (27%), Positives = 177/399 (44%), Gaps = 39/399 (9%)
Query: 17 VIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAE 76
VIY DN AT+ V+EA+ Y Y N +H + +R+ VA + A+
Sbjct: 3 VIYLDNNATTKVDPEVVEAIMPYLTDYYGNPS-SMHTFGGQLGKAVRTAREQVAALLGAD 61
Query: 77 FDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
EI FT +E N + I+TT EH + L ++L + +G + ++
Sbjct: 62 ESEIVFTSCGTEGDNAAIRAALLAQPEKRHIITTQVEHPAVLNVCKQL-ETQGYTVTYLS 120
Query: 137 GDDEGNLDLADAEKKIKG-AKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSV 195
+ G LDL + E + G LV + + +N G + +EE+GK VKE GAIF VDA Q+V
Sbjct: 121 VNGHGQLDLDELEASLTGNTALVTIMYANNETGTVFPIEEIGKRVKERGAIFHVDAVQAV 180
Query: 196 GHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCY 255
G + +++K D L SGHK P GIG LY+ + F P LIGG
Sbjct: 181 GKIPLNMKTSTIDMLTISGHK-IHAPKGIGALYVRRGV--RFRPLLIGG----------- 226
Query: 256 KLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGL----DELE 311
AGT N+ G +GL + +E I I+ K+E +L R + L + E
Sbjct: 227 ---HQERGRRAGTENVPGIVGLGKAAE-LELIHIETAIKKETRLRDRLEQTLLAKIPDCE 282
Query: 312 IPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMK--------- 362
+ + L + + + L+++ I SG C ++
Sbjct: 283 VNGDVTQRL---PNTTNIGFKYIEGEAILLSLNKYGICASSGSACTSGSLEPSHVLRAMG 339
Query: 363 --RLKINGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
++G++R S Y + +++ + V+ E+V+ LR+
Sbjct: 340 LPYTTLHGSIRFSLCRYTTEAQIDRVIEVMPEIVERLRA 378
>gi|11498172 nifS protein, class-V aminotransferase (nifS-2) [Archaeoglobus
fulgidus] >gi|7447238|pir||D69320 iron-sulfur cofactor
synthesis protein nifS-2 - Archaeoglobus fulgidus
>gi|2650055|gb|AAB90671.1| (AE001065) nifS protein,
class-V aminotransferase (nifS-2) [Archaeoglobus
fulgidus]
Length = 382
Score = 125 bits (310), Expect = 1e-27
Identities = 106/396 (26%), Positives = 184/396 (45%), Gaps = 38/396 (9%)
Query: 19 YFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEFD 78
YFD T+ + V+EAM Y N VH A + +E+R+ VA +N
Sbjct: 3 YFDYTSAKPVDERVLEAMLPYMTESFGNPS-SVHSYGFKAREAVQEAREKVAKLVNGGGG 61
Query: 79 EIAFTKNTSESLNLVALGLE-HLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEG 137
+ FT +E+ NL +G +KG I+ + EH S + P + +K+G ++E+I
Sbjct: 62 TVVFTSGATEANNLAIIGYAMRNARKGKHILVSAVEHMSVINP-AKFLQKQGFEVEYIPV 120
Query: 138 DDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVG 196
G +D++ ++K++ LV+VQH +N +G I VEE+ +++ + A+ +DA SVG
Sbjct: 121 GKYGEVDVSFIDQKLRDDTILVSVQHANNEIGTIQPVEEISEVLAGKAALH-IDATASVG 179
Query: 197 HMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCYK 256
+EVDV+K+ AD L S + GP G+G L+I KE +P ++GGG
Sbjct: 180 QIEVDVEKIGADMLTISSN-DIYGPKGVGALWIRKE--AKLQPVILGGG----------- 225
Query: 257 LTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQE--RKLVKRTTEGLDELEIPW 314
+G+ N+ +G K E ++ E+ E R+L R + + ++E +
Sbjct: 226 ---QENGLRSGSENVPSIVGFG---KAAEITAMEWREEAERLRRLRDRIIDNVLKIEESY 279
Query: 315 YGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPV-----------MKR 363
+ V+ + + LD I +G C+ +K
Sbjct: 280 LNGHPEKRLPNNVNVRFSYIEGESIVLSLDMAGIQASTGSACSSKTLQPSHVLMACGLKH 339
Query: 364 LKINGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
+ +GT+ + YN+ E+V+ L VL +++ LRS
Sbjct: 340 EEAHGTLLLTLGRYNTDEDVDRLLEVLPGVIERLRS 375
>sp|P12623|NIFS_ANASP NIFS PROTEIN >gi|97706|pir||C34443 nitrogenase cofactor synthesis
protein nifS - Anabaena sp >gi|142037|gb|AAA22006.1|
(J05111) nifS [Anabaena PCC7120]
Length = 400
Score = 124 bits (308), Expect = 2e-27
Identities = 109/399 (27%), Positives = 176/399 (43%), Gaps = 39/399 (9%)
Query: 17 VIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAE 76
VIY DN AT+ V+EA+ Y Y N +H + +R+ VA + A+
Sbjct: 3 VIYLDNNATTKVDPDVVEAIMPYLTDYYGNPS-SMHTFGGQLGKAVRTAREQVAALLGAD 61
Query: 77 FDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
EI FT +E N I+TT EH + L ++L + +G + ++
Sbjct: 62 ESEIVFTSCGTEGDNAAIRAALLAQPAKRHIITTQVEHPAVLNVCKQL-ETQGYTVTYLS 120
Query: 137 GDDEGNLDLADAEKKIKG-AKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSV 195
+ G LDL + E + G LV + + +N G + +EE+GK VKE GAIF VDA Q+V
Sbjct: 121 VNSHGQLDLDELEASLTGNTALVTIMYANNETGTVFPIEEIGKRVKERGAIFHVDAVQAV 180
Query: 196 GHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCY 255
G + +++K D L SGHK P GIG LY+ + F P LIGG
Sbjct: 181 GKIPLNMKTSTIDMLTISGHK-IHAPKGIGALYVRRGV--RFRPLLIGG----------- 226
Query: 256 KLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGL----DELE 311
AGT N+ G +GL + +E I I+ K+E +L R + L + E
Sbjct: 227 ---HQERGRRAGTENVPGIVGLGKAAE-LELIHIETAIKKETRLRDRLEQTLLAKIPDCE 282
Query: 312 IPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMK--------- 362
+ + L + + + L+++ I SG C ++
Sbjct: 283 VNGDITQRL---PNTTNIGFKYIEGEAILLSLNKYGICASSGSACTSGSLEPSHVLRAMG 339
Query: 363 --RLKINGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
++G++R S Y + +++ + V+ E+V+ LR+
Sbjct: 340 LPYTTLHGSIRFSLCRYTTEAQIDRVIEVMPEIVERLRA 378
>pir||E82231 NifS-related protein VC1184 [imported] - Vibrio cholerae (group O1
strain N16961) >gi|9655661|gb|AAF94343.1| (AE004198)
NifS-related protein [Vibrio cholerae]
Length = 416
Score = 123 bits (307), Expect = 3e-27
Identities = 110/418 (26%), Positives = 191/418 (45%), Gaps = 44/418 (10%)
Query: 4 PEDVRKDIPLTQE------VIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQM 57
PE VR+ P + V++FD S P+ V++AM Y RY AN+ G H S
Sbjct: 8 PESVRQQFPALAQIVNDKPVVFFDGPGGSQVPQSVLDAMVAYLGRYNANL--GGHYFSSK 65
Query: 58 ATQKYEES-RKVVADFINAEFDE-IAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHH 115
T + +S R+ V +NA E I F N + + + ++ GD+++ T +H+
Sbjct: 66 KTVELVQSAREHVQALLNASTPESIVFGANMTSLTFQFSRAISRDWRVGDEVIVTALDHY 125
Query: 116 SNLLPWQRLAKKKGLKLEFIEGDD-EGNLDLADAEKKIKG-AKLVAVQHVSNALGVIHEV 173
SN+ WQ+ A+ KG + + + LD A I +LVAV SN G + E+
Sbjct: 126 SNVSSWQQAAQDKGAIVHQARVNTADCTLDQAHLLSLINPRTRLVAVTFASNTTGSLVEL 185
Query: 174 EELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEF 233
E + + + GA+ VDA + H DV+ L DFLA S +K GP +G++Y+ ++
Sbjct: 186 EAIIEAAHQVGAMVYVDAVHYLPHHLPDVQALGCDFLACSAYKF-FGP-HVGIVYVAPQW 243
Query: 234 FDVFEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIG----- 288
+ P + T + P RFE GT + G G+ A ++Y+ + G
Sbjct: 244 QNTILPYKVEPAT-----------NQGPGRFETGTLSFEGLAGVIAVVQYLAQWGDPNSR 292
Query: 289 --------IDKIEKQERKLVKRTTEGLDEL-EIPWYGPRNLD--KHAGVVSFNVPPLHPH 337
+ ++ E++L + + L ++ + +G + D K + P
Sbjct: 293 LRERLVESYQQYQQHEQRLSEYFLQKLSDMPALKLHGLQLADAAKRTPTFALTCTDCTPQ 352
Query: 338 DVASVLDEHKIMVRSGHHCALPVMKRLKI---NGTVRASFHVYNSLEEVETFLGVLEE 392
+A +L E + V +GH AL ++++L + G +R YN+ EE++ +L +
Sbjct: 353 SMAKMLGEQNVCVWNGHFYALGLVRQLGLESSGGVLRIGLMHYNTTEEIDHLFALLRD 410
>gb|AAA93018.1| (U49859) NifS2 [Anabaena variabilis]
Length = 398
Score = 122 bits (302), Expect = 1e-26
Identities = 109/401 (27%), Positives = 177/401 (43%), Gaps = 44/401 (10%)
Query: 17 VIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAE 76
VIY DN AT+ V+ AM Y + N +H + +++R VA + AE
Sbjct: 3 VIYLDNNATTQVDAEVLAAMLPYLTEFYGNPS-SMHTFGGQVGKAVQQARGQVAFLLGAE 61
Query: 77 FDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
EI FT +E N + I+TT EH + + ++L +K+G K+ ++
Sbjct: 62 ESEIIFTSCGTEGNNAAIKAALAAQPEKRHIITTQVEHAAIINVCKQL-EKQGYKVTYLS 120
Query: 137 GDDEGNLDLADAEKKIKG-AKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSV 195
D +G +DL + E + G LV+ + +N GV+ +E++G K+ GAIF VDA Q+V
Sbjct: 121 VDSQGQIDLLELEAALTGDTALVSTMYANNETGVVFPIEQIGLRAKDAGAIFHVDAVQAV 180
Query: 196 GHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCY 255
G + +++K D L SGHK P GIG LYI + F P L+GG
Sbjct: 181 GKVPLNLKTSSIDMLTLSGHK-LHAPKGIGALYIRRGV--RFRPLLVGG----------- 226
Query: 256 KLTEPPERFEAGTPNIGG--AIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGL----DE 309
AGT N+ G A+G AA + I K K+E+ L R +GL
Sbjct: 227 ---HQERGRRAGTENVPGMIALGKAAELAQIHL----KDVKREKALRDRLEQGLLSAIPN 279
Query: 310 LEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMKRLKI--- 366
E+ + L + + + L+++ I SG C ++ +
Sbjct: 280 TEVNGHPTSRLPTPTNI---GFKYIEGEAILLSLNKYGICASSGSACTSGSLESSHVLRA 336
Query: 367 --------NGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
+G++R S Y + E++ L ++ +V+ LR+
Sbjct: 337 MGLPYTILHGSIRFSLSRYTTEAEIDKVLQLMPPIVERLRA 377
>dbj|BAB04979.1| (AP001511) Fe-S cluster formation protein [Bacillus halodurans]
Length = 386
Score = 121 bits (301), Expect = 1e-26
Identities = 109/411 (26%), Positives = 182/411 (43%), Gaps = 55/411 (13%)
Query: 16 EVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINA 75
E IY D+ ATS VI+AM YY N +H+ + A Q +E+R +A + A
Sbjct: 2 EPIYLDHAATSPVHPEVIQAMLPYYEEQFGNPS-SIHQFGRRARQGVDEARGTIARLLQA 60
Query: 76 EFDEIAFTKNTSESLNLVALGLEHLFK-KGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEF 134
+ E FT +E+ NL G + + KG+ I+T+ EHH+ L Q L + +G ++ +
Sbjct: 61 DPSEFIFTSGGTEADNLAIFGYAYQHRGKGNHIITSQVEHHAVLHACQEL-EHQGFEVTY 119
Query: 135 IEGDDEGNLDLADAEKKIKGAK-LVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQ 193
+ D G + + D + ++ LV + + +N +G I + E+G ++++ A+ DA Q
Sbjct: 120 VPVDQTGRVSVEDVRQALRDDTILVTLMYGNNEVGTIQPIAEIGALLQDHQAVLHTDAVQ 179
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELC 253
+ G + +++ L D L+ S HK GP G+G+LY+ V +P L GG
Sbjct: 180 AFGAISIELDHLPVDMLSVSAHK-INGPKGVGLLYVRDGI--VLKPALYGG--------- 227
Query: 254 CYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELE-- 311
E + AGT N+ IG A + +I I E++++ D+ +
Sbjct: 228 -----EQERKKRAGTENVAAIIGFAKAV----EIAIANREERQKAYFDYCQTFFDQFQQE 278
Query: 312 ---------IPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCAL---- 358
W P H VSF P +H + LD I SG C
Sbjct: 279 GVQFVMNGHQTWRLP-----HIFNVSF--PGVHVEALLVNLDLAGIAASSGSACTAGSIE 331
Query: 359 PVMKRLKINGT--------VRASFHVYNSLEEVETFLGVLEELVKSLRSSQ 401
P + ++G+ VR SF + N+ E V+ ++VK L+ +
Sbjct: 332 PSHVLVAMHGSDSELVTSGVRFSFGLGNTKEHVQWAAKETAKIVKRLQDQE 382
>pir||S76131 nitrogenase cofactor synthesis protein nifS - Synechocystis sp.
(strain PCC 6803) >gi|1001483|dbj|BAA10109.1| (D63999)
NifS protein [Synechocystis sp.]
Length = 386
Score = 121 bits (300), Expect = 2e-26
Identities = 93/342 (27%), Positives = 168/342 (48%), Gaps = 21/342 (6%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+YFDN AT+ V+EAM Y N H A+ +++R+ +A+ + A+
Sbjct: 5 LYFDNHATTALDPRVLEAMLPYLTEQYGNAG-SAHFYGWQASAAIKQARQEIAETMGAQP 63
Query: 78 DEIAFTKNTSESLNLVALGL-EHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
+EI FT +E+ NL G+ E F +G +VT EH + L P + L + G ++ ++
Sbjct: 64 EEIIFTSGATEANNLAIRGVAEAYFAQGKHLVTVETEHQAVLAPCRYL-ETLGFEVTYLS 122
Query: 137 GDDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSV 195
G +DL + EK ++ LV+V +N +GV+ ++E+G + ++ IF D AQ++
Sbjct: 123 VQSSGLVDLTELEKALRPDTILVSVMAANNEIGVLQPLKEIGALCRQRSIIFHCDGAQAL 182
Query: 196 GHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCY 255
G + +DV +L D L+F+GHK GP GIG LY + P L+GGG
Sbjct: 183 GKIPLDVHQLNIDLLSFTGHK-IHGPKGIGGLYRRQNPGVRLAPQLLGGG---------- 231
Query: 256 KLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELEIPWY 315
F +GT + +GLA + + + + ++Q R+L + +GL ++
Sbjct: 232 ----QEGNFRSGTLPVPLIVGLAKALTIAGETLVSEGDRQ-RQLRDQLWQGLAKISGVVL 286
Query: 316 GPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCA 357
+ G ++ ++ + P + + L + ++ + SG C+
Sbjct: 287 NGDYEQRLPGNLNVSITGVDPKALLTTL-QPRLALSSGSACS 327
>gb|AAC49936.1| (AF000116) tRNA splicing protein [Candida maltosa]
Length = 305
Score = 121 bits (300), Expect = 2e-26
Identities = 68/214 (31%), Positives = 111/214 (51%), Gaps = 1/214 (0%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
IY D AT+ V++ M E+Y N H H ++ E++R +AD INA+
Sbjct: 85 IYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARGYIADVINADP 144
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEG 137
EI FT + + N+ G+ +KK K + T H +L R + +G + ++
Sbjct: 145 KEIIFTSGATATNNMAIKGVPRFYKKTKKHIITTQTEHKCVLDSARHMQDEGFDITYLPV 204
Query: 138 DDEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVGH 197
+ EG ++L D + K LV+V V+N +GVI ++E+G++ +E F DAAQ+ G
Sbjct: 205 NSEGLINLDDLKAIRKDTVLVSVMAVNNEIGVIQPLKEIGQICRENKIFFHTDAAQAYGK 264
Query: 198 MEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINK 231
+ +DV ++ D L+ S HK GP GIG Y+ +
Sbjct: 265 IPIDVNEMNIDLLSISSHK-IYGPKGIGACYVRR 297
>pir||E69981 iron-sulfur cofactor synthesis protein yrvO - Bacillus subtilis
>gi|2635197|emb|CAB14693.1| (Z99117) similar to NifS
protein homolog [Bacillus subtilis]
>gi|2635215|emb|CAB14710.1| (Z99118) similar to NifS
protein homolog [Bacillus subtilis]
Length = 346
Score = 120 bits (298), Expect = 3e-26
Identities = 89/283 (31%), Positives = 139/283 (48%), Gaps = 21/283 (7%)
Query: 16 EVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINA 75
E IY D+ ATS + V+E M ++ N +H + + + +E+R +A I A
Sbjct: 2 ERIYLDHAATSPMDERVLEQMIPHFSGSFGNPS-SIHSFGRESRKWVDEARAQIAAEIGA 60
Query: 76 EFDEIAFTKNTSESLNLVALGLEHLFKK-GDKIVTTPYEHHSNLLPWQRLAKKKGLKLEF 134
EI FT +E+ NL +G K G I+TT EHH+ L ++L + G + +
Sbjct: 61 AEQEIIFTSGGTEADNLAIMGTALARKDLGRHIITTKIEHHAVLHTCEKL-EGDGFDITY 119
Query: 135 IEGDDEGNLDLADAEKKIKGAK-LVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQ 193
++ D G + ++ ++ LV V + +N +G + +EE+G+++KE A F DA Q
Sbjct: 120 LDVDQNGRVSAKQVKEALRDDTILVTVMYGNNEVGTVQPIEEIGELLKEHKAYFHTDAVQ 179
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELC 253
+ G + +DVK D L+ SGHK GP G G LY +K DV PL+ GG
Sbjct: 180 AFGLLPIDVKNSHIDLLSVSGHK-LNGPKGTGFLYASK---DVKLSPLLFGG-------- 227
Query: 254 CYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQE 296
E + AGT N+ G +GL IK + +K EK +
Sbjct: 228 -----EQERKRRAGTENVPGIVGLKEAIKLSSEERDEKNEKYQ 265
>pir||E81852 NifS-like aminotranfserase NMA1594 [imported] - Neisseria
meningitidis (group A strain Z2491)
>gi|7380235|emb|CAB84821.1| (AL162756) NifS-like
aminotranfserase [Neisseria meningitidis Z2491]
Length = 404
Score = 120 bits (297), Expect = 4e-26
Identities = 75/230 (32%), Positives = 120/230 (51%), Gaps = 4/230 (1%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+Y D AT+ K V E M Y N H A + E++R +A INA+
Sbjct: 7 VYLDYAATTTVDKRVAEKMIPYLTETFGNPASNSHAFGWEAEEAVEKARADIAALINADP 66
Query: 78 DEIAFTKNTSESLNLVALGLEHLFK-KGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
EI FT +ES NL G + +K KG ++T EH + +L R +++G ++ ++
Sbjct: 67 KEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKTEHKA-VLDTMRELERQGFEVTYLG 125
Query: 137 GDDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSV 195
+ G +DL + + I+ LV+V +N +GV+ ++ +G++ +E +F VDAAQ+
Sbjct: 126 VQENGLIDLEELKAAIRDDTILVSVMWANNEIGVVQDIPAIGEICRERKIVFHVDAAQAC 185
Query: 196 GHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
G + VDV+ K D L+ S HK GP GIG LY+ ++ E + GGG
Sbjct: 186 GKVPVDVEAAKIDLLSMSAHK-VYGPKGIGALYVRRKPRVRLEAQMHGGG 234
>sp|P55690|NIFS_RHISN NIFS PROTEIN >gi|2182702|gb|AAB91919.1| (AE000104) NifS [Rhizobium
sp. NGR234]
Length = 387
Score = 119 bits (296), Expect = 5e-26
Identities = 117/402 (29%), Positives = 174/402 (43%), Gaps = 41/402 (10%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
IY DN AT+ V++AM ++ N +H A +R+ + I A+F
Sbjct: 4 IYLDNNATTRVDAEVLQAMLPFFADVFGNAS-SMHDAGATAGAAINVARRQLQALIGAKF 62
Query: 78 D-EIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
D EI FT +ES N L + +IVT+ EH + L L K +G K+ +
Sbjct: 63 DPEITFTSGGTESNNAAILSGLEAMPERTEIVTSAVEHPAVLTLCTHLEKTRGTKVHKVP 122
Query: 137 GDDEGNLDL-ADAEKKIKGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSV 195
D +G LDL A + +V++ +N G I V +L +M K+ GAIF DA Q++
Sbjct: 123 VDHQGRLDLDAYQDALTHRVAIVSIMWANNETGTIFPVVKLAEMAKKVGAIFHTDAVQAI 182
Query: 196 GHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCY 255
G + +D+K D L+ S HK GP G+G LYI + V LI GG E
Sbjct: 183 GKLPIDLKSTAIDMLSLSAHKF-HGPKGVGALYIKR---GVPFHALIKGGHQE------- 231
Query: 256 KLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQE---RKLVKRTTEGLDELEI 312
AGT N G +GL ++ +D ++K R R +GL E +
Sbjct: 232 ------RDRRAGTENTPGIVGLGKA----AELALDCMDKDNAIIRSFRDRLEKGLLE-RV 280
Query: 313 P--WYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHC---ALPVMKRLKIN 367
P + + + S + + +L+ H I SG C +L LK
Sbjct: 281 PQVFVMGDPVTRLPNTTSIAFEGVGGEAMQFLLNRHGIACSSGSACTSRSLSTSHVLKAM 340
Query: 368 GT--------VRASFHVYNSLEEVETFLGVLEELVKSLRSSQ 401
GT VR S YN E+++ L V+ +VK LR S+
Sbjct: 341 GTPHRQAVGAVRFSLSGYNCEEDIDRVLRVIPAVVKKLRESR 382
>pir||C71863 probable aminotransferase - Helicobacter pylori (strain J99)
>gi|4155567|gb|AAD06561.1| (AE001527) putative
AMINOTRANSFERASE [Helicobacter pylori J99]
Length = 440
Score = 118 bits (293), Expect = 1e-25
Identities = 122/429 (28%), Positives = 196/429 (45%), Gaps = 44/429 (10%)
Query: 5 EDVRKDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHR-------LSQM 57
+ V+ I L + V YFD A+ L ++E + L Y AN H + L +
Sbjct: 21 DQVKSSIILKKGVSYFDWGASGLA-SALVEKRVKSLLPYYANAHSVASKHAILMGMLLKE 79
Query: 58 ATQKYEESRKVVADF--INAEFDEIAFTKNTSESLNL-----VALGLEHLFKKG--DKIV 108
+K + S + A+ ++A + + K E L + LE K +++
Sbjct: 80 CQEKLKRSLNLSANHCVLSAGYGASSAIKKFQEILGVCIPSKTKKNLEPYLKDMALKRVI 139
Query: 109 TTPYEHHSNLLPWQRLAKKKGL-KLEFIEGDDEGNLDLADAEKKIKGA--KLVAVQHVSN 165
PYEHHSN + W ++GL ++ I ++ G LDL E+ +K LV+V SN
Sbjct: 140 VGPYEHHSNEVSW-----REGLCEVVRIPLNEHGLLDLEILEQTLKKTPNSLVSVSAASN 194
Query: 166 ALGVIHEVEELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIG 225
G++ ++E+ + KE AI +D A H + + F ++ HK G G G
Sbjct: 195 VTGILTPLKEVSSLCKEYRAILALDLANFSAHANPKDCEYQTGF--YAPHKLLGGVGGCG 252
Query: 226 VLYINKEFFDVFEPPLIG-GGTIEDVELCCYK-LTEPPERFEAGTPNIGGAIGLAAGIKY 283
+L I+K+ D PP GG I+ ++ + E P R E GTP + A +
Sbjct: 253 LLGISKDLIDTQIPPSFSAGGVIKYANRTRHEFIDELPLREEFGTPGLLQFYRSALAYQL 312
Query: 284 IEKIGIDKIEKQERKLVKRTTEGLDEL-EIPWYGPRNLDKHAGVVSFNVPPLHPHDVASV 342
++ G+D I K+E L++ GL +L I YG + GVV+FN+ + P+D+A V
Sbjct: 313 RDECGLDFIHKKENNLLRVLVYGLKDLPAINIYGNLTASR-VGVVAFNIGGISPYDLARV 371
Query: 343 LD-EHKIMVRSGHHCALP---------VMKRLKIN---GTVRASFHVYNSLEEVETFLGV 389
L E+ I R+G CA P K N G +R S H +S+ +++ L
Sbjct: 372 LSYEYAIETRAGCSCAGPYGHDLLNLNAQKSSDFNAKPGWLRVSLHFTHSINDIDYLLDS 431
Query: 390 LEELVKSLR 398
L++ VK LR
Sbjct: 432 LKKAVKKLR 440
>pir||T05896 probable iron-sulfur cofactor synthesis protein F6H11.150 -
Arabidopsis thaliana >gi|2827713|emb|CAA16686.1|
(AL021684) pyridoxal-phosphate-dependent
aminotransferase - like protein [Arabidopsis thaliana]
>gi|6686815|emb|CAB64727.1| (AJ243393) nifS-like protein
[Arabidopsis thaliana] >gi|10177330|dbj|BAB10679.1|
(AB010075) NifS-like aminotranfserase [Arabidopsis
thaliana]
Length = 453
Score = 118 bits (292), Expect = 1e-25
Identities = 73/238 (30%), Positives = 116/238 (48%), Gaps = 2/238 (0%)
Query: 9 KDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKV 68
K + ++ +Y D AT+ V +AM+ + N H H A E +R
Sbjct: 46 KGVRISGRPLYLDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQ 105
Query: 69 VADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKK 128
VA I A EI F +E+ N+ G+ H +K K V T H +L R +++
Sbjct: 106 VAKLIEASPKEIVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQE 165
Query: 129 GLKLEFIEGDDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIF 187
G ++ ++ +G +DL + I+ LV++ V+N +GV+ +EE+G + KE F
Sbjct: 166 GFEVTYLPVKTDGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPF 225
Query: 188 VVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
DAAQ++G + VDVKK ++ S HK GP G+G LY+ + EP + GGG
Sbjct: 226 HTDAAQAIGKIPVDVKKWNVALMSMSAHK-IYGPKGVGALYVRRRPRIRLEPLMNGGG 282
>pir||T44281 iron-sulfur cofactor synthesis protein iscS [imported] -
Azotobacter vinelandii >gi|2271521|gb|AAC24472.1|
(AF010139) IscS [Azotobacter vinelandii]
Length = 404
Score = 117 bits (290), Expect = 2e-25
Identities = 72/232 (31%), Positives = 125/232 (53%), Gaps = 6/232 (2%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYR--ANVHRGVHRLSQMATQKYEESRKVVADFINA 75
IY D +AT+ V + M E N H A + E +R+ VA+ +NA
Sbjct: 5 IYLDYSATTPVDPRVAQKMCECLTMEGNFGNPASRSHVFGWKAEEAVENARRQVAELVNA 64
Query: 76 EFDEIAFTKNTSESLNLVALGLEHL-FKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEF 134
+ EI +T +ES NL G+ H KG I+T+ EH + +L R +++G ++ +
Sbjct: 65 DPREIVWTSGATESDNLAIKGVAHFNASKGKHIITSKIEHKA-VLDTTRQLEREGFEVTY 123
Query: 135 IEGDDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQ 193
+E ++G + A ++ LV+V HV+N +G ++++ +G++ + G ++ VDAAQ
Sbjct: 124 LEPGEDGLITPAMVAAALREDTILVSVMHVNNEIGTVNDIAAIGELTRSRGVLYHVDAAQ 183
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
S G + +D++++K D ++FS HK GP GIG LY+ ++ E + GGG
Sbjct: 184 STGKVAIDLERMKVDLMSFSAHK-TYGPKGIGALYVRRKPRVRLEAQMHGGG 234
>pir||E64570 nifS-like protein - Helicobacter pylori (strain 26695)
>gi|2313511|gb|AAD07474.1| (AE000556) nifS-like protein
[Helicobacter pylori 26695]
Length = 440
Score = 117 bits (290), Expect = 2e-25
Identities = 122/430 (28%), Positives = 197/430 (45%), Gaps = 46/430 (10%)
Query: 5 EDVRKDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHR-------LSQM 57
E V+ I L + V YFD A+ L ++E + L Y AN H + L +
Sbjct: 21 EQVKSSIILKKGVSYFDWGASGLA-SVLVEKRVKSLLPYYANAHSVASKHAILMGMLLKE 79
Query: 58 ATQKYEESRKVVADF--INAEFDEIAFTKNTSESLNL-----VALGLEHLFKKG--DKIV 108
+K + S + A+ ++A + + K E L + LE K +++
Sbjct: 80 CQEKLKRSLNLSANHCVLSAGYGASSAIKKFQEILGVCIPSKTKKNLEPYLKDMALKRVI 139
Query: 109 TTPYEHHSNLLPWQRLAKKKGL-KLEFIEGDDEGNLDLADAEKKIKGA--KLVAVQHVSN 165
PYEHHSN + W ++GL ++ I ++ G LDL E+ +K + LV++ SN
Sbjct: 140 VGPYEHHSNEISW-----REGLCEVVRIPLNEHGLLDLEILEQTLKKSPNSLVSISAASN 194
Query: 166 ALGVIHEVEELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIG 225
G++ ++E+ + K+ A +D A H + + F ++ HK G G G
Sbjct: 195 VTGILTPLKEVSSLCKKYKAALALDLANFSAHANPKDCEYQTGF--YAPHKLLGGIGGCG 252
Query: 226 VLYINKEFFDV-FEPPLIGGGTIEDVELCCYK-LTEPPERFEAGTPNIGGAIGLAAGIKY 283
+L I+K+ D P GG I+ ++ + E P R E GTP + A +
Sbjct: 253 LLGISKDLIDTQIAPSFSAGGVIKYANRTRHEFIDELPLREEFGTPGLLQFYRSALAYQL 312
Query: 284 IEKIGIDKIEKQERKLVKRTTEGLDEL-EIPWYGPRNLDKH-AGVVSFNVPPLHPHDVAS 341
++ G+D I K+E L++ GL +L I YG NL H GVV+FN+ + P+D+A
Sbjct: 313 RDECGLDFIHKKENNLLRVLMHGLKDLPAINIYG--NLTAHRVGVVAFNIGGISPYDLAR 370
Query: 342 VLD-EHKIMVRSGHHCALP---------VMKRLKIN---GTVRASFHVYNSLEEVETFLG 388
VL E+ I R+G CA P K N G +R S H +S+ +++ L
Sbjct: 371 VLSYEYAIETRAGCSCAGPYGHDLLNLNAQKSSDFNAKPGWLRVSLHFTHSINDIDYLLD 430
Query: 389 VLEELVKSLR 398
L++ VK LR
Sbjct: 431 SLKKAVKKLR 440
>gb|AAF53143.1| (AE003634) CG12264 gene product [Drosophila melanogaster]
Length = 462
Score = 117 bits (290), Expect = 2e-25
Identities = 72/229 (31%), Positives = 111/229 (48%), Gaps = 2/229 (0%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+Y D AT+ V++AM Y + N H H E++R+ VA I A+
Sbjct: 64 LYLDAQATTPMDPRVLDAMLPYLTNFYGNPHSRTHAYGWETESAVEKAREQVATLIGADP 123
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEG 137
EI FT +ES N+ G+ + + V T H +L R + +G K+ ++
Sbjct: 124 KEIIFTSGATESNNIAVKGVARFYGTKKRHVITTQTEHKCVLDSCRALENEGFKVTYLPV 183
Query: 138 DDEGNLDLADAEKKIKG-AKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVG 196
G +DL E+ I LV++ V+N +GV V+E+GK+ + F DAAQ+VG
Sbjct: 184 LANGLIDLQQLEETITSETSLVSIMTVNNEIGVRQPVDEIGKLCRSRRVFFHTDAAQAVG 243
Query: 197 HMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
+ +DV + D ++ SGHK GP G+G LY+ + EP GGG
Sbjct: 244 KVPLDVNAMNIDLMSISGHK-IYGPKGVGALYVRRRPRVRLEPIQSGGG 291
>pir||A71652 iron-sulfur cofactor synthesis protein RP486 - Rickettsia
prowazekii >gi|3861039|emb|CAA14939.1| (AJ235272) NIFS
PROTEIN HOMOLOG (spl1) [Rickettsia prowazekii]
Length = 410
Score = 116 bits (289), Expect = 3e-25
Identities = 78/229 (34%), Positives = 113/229 (49%), Gaps = 3/229 (1%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
IY D +T+ V+EAM Y+ N H H A E +R +VA I A+
Sbjct: 12 IYMDYQSTTPIDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAENAVENARSMVAKVIGADS 71
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEG 137
EI FT +ES NLV G+ + K + T H +L R +++G+K+ ++
Sbjct: 72 KEIIFTSGATESNNLVIKGIAKFYGNKKKHIITLVSEHKCVLNACRHLEQEGIKITYLPI 131
Query: 138 DDEGNLDLADAEKKIKGAKL-VAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVG 196
G +DL + I L V+V V+N +GVI ++E+GK+ +E F D AQ G
Sbjct: 132 KSNGIIDLETLKNAITDQTLLVSVMAVNNEIGVIQPLKEIGKICRERNVFFHSDIAQGFG 191
Query: 197 HMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
+ ++V + D + SGHK GP GIG LYI K+ V PLI GG
Sbjct: 192 KIPINVNECNIDLASISGHK-IYGPKGIGALYIRKK-PRVRVTPLINGG 238
>pir||T11683 probable iron-sulfur cofactor synthesis protein NFS1 - fission
yeast (Schizosaccharomyces pombe)
>gi|3560214|emb|CAA20767.1| (AL031536) trna splicing
protein [Schizosaccharomyces pombe]
Length = 498
Score = 116 bits (287), Expect = 6e-25
Identities = 75/255 (29%), Positives = 123/255 (47%), Gaps = 13/255 (5%)
Query: 3 IPEDVRKDIPLTQ-----------EVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGV 51
IPE+ K + L Q IY D ATS V+++M ++ N H
Sbjct: 75 IPENSLKTVTLDQAQTAASTVTGLHPIYMDFQATSPLDYRVLDSMLPFFTGIYGNPHSRT 134
Query: 52 HRLSQMATQKYEESRKVVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTP 111
H A + E +R+ +A INA+ EI FT +ES N + G+ +K K + +
Sbjct: 135 HAYGWEAEKAVENARQEIASVINADPREIIFTSGATESNNAILKGVARFYKSRKKHLVSV 194
Query: 112 YEHHSNLLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVI 170
H +L R +++G ++ F+ G ++L + I+ V+V V+N +GV
Sbjct: 195 QTEHKCVLDSLRALQEEGFEVTFLPVQTNGLINLDELRDAIRPDTVCVSVMAVNNEIGVC 254
Query: 171 HEVEELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYIN 230
+EE+GK+ +++ F DAAQ G +++DV ++ D ++ S HK GP GIG Y+
Sbjct: 255 QPLEEIGKICRQKKVFFHSDAAQGYGKIDIDVNRMNIDLMSISAHK-IYGPKGIGAAYVR 313
Query: 231 KEFFDVFEPPLIGGG 245
+ EP + GGG
Sbjct: 314 RRPRVRLEPLISGGG 328
>pir||H75554 NifS-related protein - Deinococcus radiodurans (strain R1)
>gi|6457815|gb|AAF09737.1|AE001877_4 (AE001877)
NifS-related protein [Deinococcus radiodurans]
Length = 401
Score = 116 bits (287), Expect = 6e-25
Identities = 108/391 (27%), Positives = 166/391 (41%), Gaps = 34/391 (8%)
Query: 19 YFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEFD 78
Y DN A L P I+A+ E+ R+ A H Q Q +R+ A F+NAE
Sbjct: 25 YLDNAAGGLLPTRSIQAVTEHLTRFGATNAMPGHAPGQQIGQLKARAREGTALFLNAEAP 84
Query: 79 EIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEGD 138
++A + + +A L+ GD+++ + EH +N PW+ L + +
Sbjct: 85 DVALGPSATALAFRLAAAFARLWGPGDEVIVSGLEHEANASPWRELERVGVTVKVWHARA 144
Query: 139 DEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVGH 197
E L D + +LVAV SN LGV ++ + V+ G VVDA + H
Sbjct: 145 PEMRLHPDDLAALLSPRTRLVAVTAASNVLGVAPDIAAITAQVRAAGGWTVVDAVHAAPH 204
Query: 198 MEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCYKL 257
+ DV+ ADF+ FS +K GP +G L+I E P + +ED ++
Sbjct: 205 LLPDVQAWGADFVTFSPYK-VFGP-HLGGLWIRPELRPQLPWPRL--SFVEDGDI----- 255
Query: 258 TEPPERFEAGTPNIGGAIGLAAGIKYIEKIG-------------IDKIEKQERKLVKRTT 304
E GTP G + Y+ ++G ++I ER + +
Sbjct: 256 ----TGIEHGTPQYELLAGWLGTLDYLRELGGGDTLTRAALVAAFEQIAALERPVTEALL 311
Query: 305 EGLDELEIPW---YGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVM 361
GL L P+ YGP+ L+ G V+F V P D A L + V +GH A+ +
Sbjct: 312 TGL--LARPYVTVYGPQTLEGRVGTVAFQVQGEAPLDTALRLSAQGVDVAAGHFYAVQPL 369
Query: 362 KRLKI--NGTVRASFHVYNSLEEVETFLGVL 390
+ L + +G RAS Y S E+V L L
Sbjct: 370 RDLGLYPDGVARASLAHYTSEEDVARLLAAL 400
>emb|CAC08815.1| (AJ279023) putative SPL1 tRNA splicing protein [Candida rugosa]
Length = 400
Score = 115 bits (285), Expect = 1e-24
Identities = 68/229 (29%), Positives = 116/229 (49%), Gaps = 2/229 (0%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
IY D AT+ T V++ M + + N H H ++ E +R +A+ I A+
Sbjct: 3 IYLDMQATTPTDPRVVDKMLPFSIGLYGNPHSSTHSYGWETEKEVEAARAHIANVIKADP 62
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEG 137
EI FT +ES N G+ + K + + T H +L R + +G + ++
Sbjct: 63 KEIVFTSGATESNNTAIKGVARFYGKTKRHIITTQTEHKCVLDTCRRLQDEGFDVTYLAV 122
Query: 138 DDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVG 196
+++G ++L + EK I K LV+V V+N +GV+ + E+G + ++ F D+AQ+ G
Sbjct: 123 NEDGLINLEELEKAIRKDTALVSVMSVNNEIGVVQPLAEIGAICRKNKVFFHTDSAQAFG 182
Query: 197 HMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
+++DV + D ++ S HK GP GIG LY+ + EP + GGG
Sbjct: 183 RLDLDVNAQQIDLMSISSHK-IYGPMGIGALYVRRRPRVRIEPLINGGG 230
>gi|10864079 cysteine desulfurase [Homo sapiens] >gi|4185794|gb|AAD09187.1|
(AF097025) cysteine desulfurase [Homo sapiens]
Length = 457
Score = 115 bits (284), Expect = 1e-24
Identities = 71/229 (31%), Positives = 112/229 (48%), Gaps = 2/229 (0%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+Y D AT+ V++AM Y + Y N H H + E +R+ VA I A+
Sbjct: 59 LYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADP 118
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEG 137
EI FT +ES N+ G+ ++ K + T H +L R + +G ++ ++
Sbjct: 119 REIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYLPV 178
Query: 138 DDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVG 196
G +DL + E I+ LV+V V+N +GV + E+G++ F DAAQ+VG
Sbjct: 179 QKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQAVG 238
Query: 197 HMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
+ +DV +K D ++ SGHK GP G+G +YI + E GGG
Sbjct: 239 KIPLDVNDMKIDLMSISGHK-IYGPKGVGAIYIRRRPRVRVEALQSGGG 286
>gi|11420479 cysteine desulfurase [Homo sapiens]
Length = 421
Score = 115 bits (284), Expect = 1e-24
Identities = 71/229 (31%), Positives = 112/229 (48%), Gaps = 2/229 (0%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+Y D AT+ V++AM Y + Y N H H + E +R+ VA I A+
Sbjct: 80 LYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADP 139
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEG 137
EI FT +ES N+ G+ ++ K + T H +L R + +G ++ ++
Sbjct: 140 REIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYLPV 199
Query: 138 DDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVG 196
G +DL + E I+ LV+V V+N +GV + E+G++ F DAAQ+VG
Sbjct: 200 QKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQAVG 259
Query: 197 HMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
+ +DV +K D ++ SGHK GP G+G +YI + E GGG
Sbjct: 260 KIPLDVNDMKIDLMSISGHK-IYGPKGVGAIYIRRRPRVRVEALQSGGG 307
>dbj|BAB13286.1| (AP001119) hypothetical protein [Buchnera sp. APS]
Length = 404
Score = 114 bits (282), Expect = 2e-24
Identities = 67/218 (30%), Positives = 111/218 (50%), Gaps = 4/218 (1%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYL--RYRANVHRGVHRLSQMATQKYEESRKVVADFINA 75
IY D AT+ V + M Y N H+ A + + +R +++ I A
Sbjct: 5 IYLDYAATTPVEFEVAKKMMNYLTIDGVFGNSASRSHKFGWKAEEVVDIARNQISELIGA 64
Query: 76 EFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFI 135
+ EI FT +ES NL G+ + K + T H ++L R + KG + ++
Sbjct: 65 DSREIVFTSGATESNNLAIKGIASFHQNKGKHIVTSKTEHKSVLDTCRYLENKGFTVTYL 124
Query: 136 EGDDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQS 194
+ G +DL + +K IK LV++ HV+N +G+I ++ + ++ + G F VDA QS
Sbjct: 125 TPKNNGIIDLNNLKKNIKKDTILVSIMHVNNEIGIIQDINSISQICRNHGVFFHVDATQS 184
Query: 195 VGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKE 232
VG + +D+KK+ D ++FS HK GP GIG LY+ ++
Sbjct: 185 VGKIPIDLKKIPIDLMSFSAHK-IYGPKGIGGLYVRRK 221
>sp|Q10089|YAOA_SCHPO HYPOTHETICAL 48.2 KD PROTEIN C11D3.10 IN CHROMOSOME I
>gi|7492235|pir||T37521 nifS homolog - fission yeast
(Schizosaccharomyces pombe) >gi|1107899|emb|CAA92311.1|
(Z68166) nifS homolog, putative aminotransferase
[Schizosaccharomyces pombe]
Length = 434
Score = 113 bits (280), Expect = 4e-24
Identities = 99/397 (24%), Positives = 176/397 (43%), Gaps = 35/397 (8%)
Query: 8 RKDIPLTQ---EVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEE 64
R +PL Q + IY + + + V A+ Y N H M ++ EE
Sbjct: 11 RSYVPLLQGNTKTIYLNQSFQAPMNMLVSTALQGYINEGLYNPHP-----KPMWKERTEE 65
Query: 65 SRKVVADFINAEF-DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQR 123
+R ++A +NA D I FT++T+E LNL L+ +K GD +V EH + W
Sbjct: 66 TRSLLAKLLNASTKDSITFTRDTTEGLNLFQRSLK--WKPGDNVVILDNEHPNQGFGWIA 123
Query: 124 LAKKKGLKLEFIEGDDEGNLDLADAEKKIKG-AKLVAVQHVSNALGVIHEVEELGKMVKE 182
L + GL++ + + + + + + + K + + V G ++V+++ +
Sbjct: 124 L-QNDGLEVRLVPNEGQYHANASTFAPYVDSRTKAIGLSSVMFHSGQKNDVKDIANAFRP 182
Query: 183 EGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFE--PP 240
+G + D Q VG ++DV+ L AFS HKG PTG+GVLY++ PP
Sbjct: 183 KGIHVLADLTQQVGLSKIDVQDLNVSACAFSCHKGLGCPTGLGVLYVSPLAISELRSTPP 242
Query: 241 LIGGGTIEDVE-----LCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQ 295
+GGG +ED + K + R+E L A +K++ K+GI +E+
Sbjct: 243 FVGGGAVEDFKEDLKLKLNAKYHQSALRYEHTNNAYMLITALRAYLKFLLKVGISNVERY 302
Query: 296 ERKLVKRTTEGLDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHH 355
+ L K + L+ L + G ++ DKH+ H + + + E +R
Sbjct: 303 LQGLGKDLIKELESLNVSVIGYKDFDKHSS---------HSYVLKILNPEWFDFLRQQGV 353
Query: 356 CALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEE 392
C + +R SF +YN+ +++ F+ V+ +
Sbjct: 354 CV------SRFESGIRVSFGLYNTSKDIIKFISVIRK 384
>gb|AAA92036.1| (U13077) capreomycin acetyltransferase [Streptomyces capreolus]
Length = 289
Score = 111 bits (276), Expect = 1e-23
Identities = 82/290 (28%), Positives = 140/290 (48%), Gaps = 5/290 (1%)
Query: 107 IVTTPYEHHSNLLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSN 165
++ T E+ N +P A++ G ++ I D G +D+ + + + KLV++ H
Sbjct: 1 MLVTEVEYGGNAIPILHRAREVGFEVVTIPSDAVGQVDVEALRELVDERVKLVSLVHAPT 60
Query: 166 ALGVIHEVEELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIG 225
G+++ V E+ E GA+ ++DA QS+G + V +L D ++ +G K GP G G
Sbjct: 61 NGGLVNPVREVASAAHEVGALVLLDACQSLGQLPVRADELGVDIISATGRKWLRGPRGTG 120
Query: 226 VLYINKEFFDVFEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIE 285
VL + + D P L+ + E V KL E +E ++ +GL A +Y
Sbjct: 121 VLVVCRSAADRLRPRLVDLHSGEWVGPEEVKLREDARVYELWETSVADRLGLIAAARYAL 180
Query: 286 KIGIDKIEKQERKLVKRTTEGLDELEIPWYGPRNLD-KHAGVVSFNVPPLHPHDVASVLD 344
+GID I + R +R GL E+P R++ + +G+V+F V P V L
Sbjct: 181 DLGIDAIAAEVRSRAERLRAGL--REVPGVEVRDIGARLSGIVTFTVEGRDPVAVRDELA 238
Query: 345 EHKIMVRSGHHCALPV-MKRLKINGTVRASFHVYNSLEEVETFLGVLEEL 393
H ++V + H + + M R + G VR S H + S E+V+ + V+ L
Sbjct: 239 RHDVVVTTSHLTSTRIDMTRRGVEGLVRMSPHYFVSPEQVDRAVEVVAAL 288
>pir||T11709 nifS protein homolog - fission yeast (Schizosaccharomyces pombe)
>gi|3560240|emb|CAA20707.1| (AL031532) nifs homolog,
putative aminotransferase [Schizosaccharomyces pombe]
Length = 396
Score = 111 bits (276), Expect = 1e-23
Identities = 80/274 (29%), Positives = 137/274 (49%), Gaps = 12/274 (4%)
Query: 60 QKYEESRKVVADFINAEF-DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNL 118
QK EE+R++VA ++NA D IAFT++T+E LNL + F+ GD +V EH ++
Sbjct: 64 QKAEETRELVAKYLNASSSDSIAFTRDTTEGLNLFQRSMH--FQPGDNVVLLDGEHPNHG 121
Query: 119 LPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEELG 177
W L ++ GL++ I D D + E + + K + + V G ++ V+++
Sbjct: 122 FGWISL-QEAGLEVRLIPSKDIYYADASTFEPYVDEKTKAIGISSVMFHSGQLNNVKDIC 180
Query: 178 KMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVF 237
+ E +VD Q VG +DV++L AF+ HKG PTG+GVLY+ +
Sbjct: 181 NKFRPENIHVLVDMTQQVGLSSIDVQELNVSACAFACHKGLSCPTGLGVLYVAPDVLPQL 240
Query: 238 E--PPLIGGGTIEDVELCCYKLTEP-----PERFEAGTPNIGGAIGLAAGIKYIEKIGID 290
+ PP++G G IE+++ P RFE ++ + L + ++ IG +
Sbjct: 241 KNVPPIVGAGAIENLDANLLVNLNPIYFSTARRFEHLNKSLITTLCLNNYLSFLMDIGFE 300
Query: 291 KIEKQERKLVKRTTEGLDELEIPWYGPRNLDKHA 324
IE+ R+L K + L +L + G ++ K +
Sbjct: 301 NIEQYLRELGKSLVDELSKLGVTIIGSKDPKKRS 334
>gi|6754846 nitrogen fixation gene, yeast homolog 1 (S. cerevisiae); nifS-like
(sic) [Mus musculus] >gi|4138201|emb|CAA10916.1|
(AJ222660) mouse NifS-like protein [Mus musculus]
Length = 459
Score = 111 bits (276), Expect = 1e-23
Identities = 70/229 (30%), Positives = 111/229 (47%), Gaps = 2/229 (0%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+Y D AT+ V++AM Y + Y N H H + E +R+ VA I A+
Sbjct: 61 LYMDVQATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGADP 120
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEG 137
EI FT +ES N+ G+ ++ K + T H +L R + +G ++ ++
Sbjct: 121 REIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYLPV 180
Query: 138 DDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVG 196
G +DL + E I+ LV+V V+N +GV + E+ ++ F DAAQ+VG
Sbjct: 181 QKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIRQICSSRKVYFHTDAAQAVG 240
Query: 197 HMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
+ +DV +K D ++ SGHK GP G+G +YI + E GGG
Sbjct: 241 KIPLDVNDMKIDLMSISGHK-LYGPKGVGAIYIRRRPRVRVEALQSGGG 288
>sp|Q57337|NIFS_HAEIN NIFS PROTEIN HOMOLOG >gi|1075069|pir||D64064 iron-sulfur cofactor
synthesis protein HI0378 - Haemophilus influenzae
(strain Rd KW20) >gi|1573347|gb|AAC22035.1| (U32721)
nifS protein (nifS) [Haemophilus influenzae Rd]
Length = 406
Score = 111 bits (274), Expect = 2e-23
Identities = 90/364 (24%), Positives = 173/364 (46%), Gaps = 38/364 (10%)
Query: 52 HRLSQMATQKYEESRKVVADFINAEFDEIAFTKNTSESLNLVALGLEHLFK-KGDKIVTT 110
H+ A + + +R +AD I A+ EI FT +ES NL G H ++ KG I+T
Sbjct: 43 HKFGWQAEEAVDIARNQIADLIGADSREIVFTSGATESDNLAIKGAAHFYQTKGKHIITC 102
Query: 111 PYEHHSNLLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKGAKLVA-VQHVSNALGV 169
EH + +L R +++G ++ ++ + +G +DL + ++ ++A + H +N +GV
Sbjct: 103 KTEHKA-VLDTCRQLEREGFEVTYLSPEADGLIDLEKFKAALRPDTILASIMHANNEIGV 161
Query: 170 IHEVEELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYI 229
+ +++ +G++ + IF VDA QSVG +E+++++L D ++ S HK GP G+G LY+
Sbjct: 162 LQDIKAIGELCRANKTIFHVDATQSVGKVEINLEELAVDLMSMSSHK-LYGPKGVGALYV 220
Query: 230 NKEFFDVFEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGI 289
++ E + GGG +GT + +G+ + I K +
Sbjct: 221 RRKPRVRLEAIIHGGG--------------HERGMRSGTLPVHQIVGMGEAYR-IAKEEM 265
Query: 290 DKIEKQERKLVKRTTEGLDELEIPWYG---PRNLDKHAGVVSFNVPPLHPHDVASVLDEH 346
+ + L R GL ++E + LD + +SFN + + ++
Sbjct: 266 ASEMPRLKALRDRLYNGLKDIEETYVNGSMEHRLDSNLN-ISFN----YVEGESLMMALR 320
Query: 347 KIMVRSGHHCALP------VMKRLKIN-----GTVRASFHVYNSLEEVETFLGVLEELVK 395
I V SG C V++ L +N ++R + Y + EE++ + +++ V+
Sbjct: 321 DIAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFTLGRYTTEEEIDYTINLMKGAVE 380
Query: 396 SLRS 399
LR+
Sbjct: 381 KLRA 384
>gb|AAG27074.1|AF105225_10 (AF105225) NifS [Acetobacter diazotrophicus]
Length = 400
Score = 109 bits (269), Expect = 7e-23
Identities = 102/396 (25%), Positives = 172/396 (42%), Gaps = 35/396 (8%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+Y DN AT+ + AM ++ N +H +R+ + + AEF
Sbjct: 4 VYLDNNATTRVDPAAVSAMLPFFTEQFGNAS-SMHAFGAEVGGALHTARRQLQALLGAEF 62
Query: 78 D-EIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
D EI +T +ES N L ++IVT+ EH + L L K +G ++ +I
Sbjct: 63 DHEIVYTAGGTESDNTAILSALETQAGRNEIVTSAVEHPAVLALCAWLEKTRGTRVHYIG 122
Query: 137 GDDEGNLDLADAEKKIKGAK--LVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQS 194
D G LD+ DA ++ + + ++ +N G I VE L + E GA+F DA Q+
Sbjct: 123 VDARGRLDI-DAYRRALSPRTAIASIMWANNETGTIFPVETLAAVAHEAGALFHTDAVQA 181
Query: 195 VGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCC 254
VG + ++++ + D L+ SGHK P GIG LY+ + V PLI GG E
Sbjct: 182 VGKIPMNLRHSEIDMLSLSGHK-LHAPKGIGALYVRR---GVRLRPLIRGGHQE------ 231
Query: 255 YKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEG-LDELEIP 313
AGT N+ G IGL + + + ID+ + R L R +G L+ +
Sbjct: 232 -------RGRRAGTENVPGIIGLGVAAE-LARHHIDEENTRVRALRDRLEQGLLERIGNA 283
Query: 314 WYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCA------LPVMKRLKI- 366
W ++ + + + ++++ I SG C V++ + +
Sbjct: 284 WVTGDTENRLPNTANVAFEFIEGEAILLLMNKAGIAASSGSACTSGSLEPSHVLRAMHVP 343
Query: 367 ----NGTVRASFHVYNSLEEVETFLGVLEELVKSLR 398
+G +R SF N+ +V+ L V+ ++ LR
Sbjct: 344 YTAAHGAIRFSFSRENTDADVDRVLDVMPGIIAKLR 379
>gb|AAF82635.1|AF167538_2 (AF167538) NifS [Trichodesmium sp. IMS101]
Length = 378
Score = 108 bits (267), Expect = 1e-22
Identities = 98/362 (27%), Positives = 157/362 (43%), Gaps = 33/362 (9%)
Query: 51 VHRLSQMATQKYEESRKVVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTT 110
+H + ++R VA +NAE EI FT +E N I+TT
Sbjct: 15 MHTFGGKVGKATRKARSQVAALLNAEDTEIIFTSCGTEGDNAAIRAALTAQPNKRHIITT 74
Query: 111 PYEHHSNLLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKG-AKLVAVQHVSNALGV 169
EH + L + L +K+G + ++ D +G +DL + E I G LV+V + +N GV
Sbjct: 75 QVEHPAALSLCKYL-EKQGYTVTYLSVDSQGMIDLTELEAAITGNTALVSVMYANNETGV 133
Query: 170 IHEVEELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYI 229
+ +E++G++ KE GA+F VD Q+VG + +D+K D LA SGHK P GI LY+
Sbjct: 134 VFPIEKIGQIAKEYGALFHVDGVQAVGKVPLDMKNSTIDMLALSGHK-LHAPKGIAALYV 192
Query: 230 NKEFFDVFEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGI 289
+ F P LIGG AGT N+ G I L + + K +
Sbjct: 193 RRG--TRFRPLLIGG--------------HQERGRRAGTENVPGIIALGKACE-LAKEHL 235
Query: 290 DKIEKQERKLVKRTTEG-LDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKI 348
I+K ER+L G L+ + + + + + + L + I
Sbjct: 236 AHIDK-ERELRDLLENGILNTIPDCAVNGHLIQRLPNTTNIGFKYIEGEAILLHLSHYGI 294
Query: 349 MVRSGHHCALPVMKRLKI-----------NGTVRASFHVYNSLEEVETFLGVLEELVKSL 397
SG C ++ + +G++R S Y + +EVE L V+ + + L
Sbjct: 295 CASSGSACTSGSLEPSHVLRAMGLPYTVLHGSIRFSLSRYTTRQEVEEVLAVMPSIAERL 354
Query: 398 RS 399
R+
Sbjct: 355 RA 356
>pir||G81350 probable aminotransferase Cj0791c [imported] - Campylobacter jejuni
(strain NCTC 11168) >gi|6968237|emb|CAB73056.1|
(AL139076) putative aminotransferase [Campylobacter
jejuni]
Length = 424
Score = 108 bits (267), Expect = 1e-22
Identities = 117/426 (27%), Positives = 190/426 (44%), Gaps = 44/426 (10%)
Query: 6 DVRKDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEES 65
D++K++ L + +++FD TA++L K V + + + Y AN H S Q YE++
Sbjct: 7 DLKKELILKKGILHFDFTASALALKCVEKEILKILPTY-ANTHSDSSLNSFKTQQTYEQA 65
Query: 66 RKVVADFINAE--FDEIAFTKNTSESLN---------LVALGLEHLFKKGDK-----IVT 109
RK + ++ + F IA +S ++ + L E F + DK ++
Sbjct: 66 RKDIKKSLSLDENFALIACGTGSSSAIKKFQELIGIYIPPLVKERYFTQIDKNTLPLVIV 125
Query: 110 TPYEHHSNLLPWQRLAKKKGL-KLEFIEGDDEGNLDLADAEK---KIKGAKLVA-VQHVS 164
PYEHHSN L+ ++GL + I D G +D EK K K K++A S
Sbjct: 126 GPYEHHSN-----ELSFREGLCECIRIPLDKNGEIDFDFLEKTLQKNKKRKIIASFSLAS 180
Query: 165 NALGVIHEVEELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGI 224
N G++ + + + +MV++ I DA+ + + + + A F+ S HK G G
Sbjct: 181 NVTGILSDYKRISEMVRKFKGIVAFDASSFIPYKNISCQYYDALFI--SSHKLIGGIGGS 238
Query: 225 GVLYINKEFFDVFEPPLIGGGTIEDVELC--CYKLTEPPERFEAGTPNIGGAIGLAAGIK 282
G+L I K+ +P GGT+ V CY E E GTP I I + K
Sbjct: 239 GLLAIKKDLCG-NKPSFAAGGTVGYVSRTSQCYLCNEEALE-EGGTPGILQLIRASLAFK 296
Query: 283 YIEKIGIDKIEKQERKLVKRTTEGLDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASV 342
+ IG+ IEK+E L E L + +NL + +FN+ + P D+A
Sbjct: 297 IKDSIGVKNIEKKEEILKDYFFEKLKTIPNLILYAKNLKTRLPIFAFNIKGISPFDIAYE 356
Query: 343 LD-EHKIMVRSGHHCALPV---MKRLKIN-------GTVRASFHVYNSLEEVETFLGVLE 391
L ++ I R+G CA P + LK N G +R S H + E+++ F L
Sbjct: 357 LSKKYHIETRAGCACAGPYGHDLLGLKDNQKLKTKPGWLRISLHYTHEKEDIDYFFNALN 416
Query: 392 ELVKSL 397
+ + L
Sbjct: 417 KTIVKL 422
>pir||S02507 nitrogenase cofactor synthesis protein nifS - Klebsiella pneumoniae
>gi|43831|emb|CAA31675.1| (X13303) nifS protein (AA
1-400) [Klebsiella pneumoniae]
Length = 400
Score = 106 bits (263), Expect = 4e-22
Identities = 101/397 (25%), Positives = 171/397 (42%), Gaps = 37/397 (9%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+Y DN AT+ V+EAM + + N +H A E + + A + AE+
Sbjct: 4 VYLDNNATTRLDPMVLEAMMPFLTDFYGNPS-SIHDFGIPAQAALERAHQQAAALLGAEY 62
Query: 78 -DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
EI FT +E+ L + +I+T+ EH + L + + +++G ++ I
Sbjct: 63 PSEIIFTSCATEATATAIASAIALLPERREIITSVVEHPATLAACEHM-EREGYRIHRIA 121
Query: 137 GDDEGNLDLADAEKKIKG-AKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSV 195
D EG LD+A + LV+V +N GV+ + E+ ++ E+GA+F DA Q V
Sbjct: 122 VDGEGALDMAQFRAALSPRVALVSVMWANNETGVLFPIGEMAELAHEQGALFHCDAVQVV 181
Query: 196 GHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCY 255
G + + V + + D L+ S HK GP G+G LY+ + F P L GG
Sbjct: 182 GKIPIAVGQTRIDMLSCSAHKF-HGPKGVGCLYLRRG--TRFRPLLRGG----------- 227
Query: 256 KLTEPPERFEAGTPNIGGAIGLAAG--IKYIEKIGIDKIEKQERKLVKRTTEGLDELEIP 313
AGT NI G +G+ A + I G+ I + +L R + + +
Sbjct: 228 ---HQEYGRRAGTENICGIVGMGAACELANIHLPGMTHIGQLRNRLEHRLLASVPSVMVM 284
Query: 314 WYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCA------LPVMKRLKI- 366
G + G V+ + + +L++ I SG C VM+ + I
Sbjct: 285 GGGQPAV---PGTVNLAFEFIEGEAILLLLNQAGIAASSGSACTSGSLEPSHVMRAMNIP 341
Query: 367 ----NGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
+GT+R S Y +E++ + L ++ LR+
Sbjct: 342 YTAAHGTIRFSLSRYTREKEIDYVVATLPPIIDRLRA 378
>pir||C83387 probable pyridoxal-phosphate dependent enzyme PA2062 [imported] -
Pseudomonas aeruginosa (strain PAO1)
>gi|9948071|gb|AAG05450.1|AE004633_1 (AE004633) probable
pyridoxal-phosphate dependent enzyme [Pseudomonas
aeruginosa]
Length = 393
Score = 106 bits (263), Expect = 4e-22
Identities = 73/232 (31%), Positives = 118/232 (50%), Gaps = 7/232 (3%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRA---NVHRGVHRLSQMATQKYEESRKVVADFIN 74
+YFD AT+ + VI AM E L Y+A N H + A + E +R+ VA +
Sbjct: 6 LYFDYAATTPVDERVIRAMVEC-LGYQACFGNPASNSHAYGRQARRAVERAREQVAALVG 64
Query: 75 AEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEF 134
A +I +T +ES NL G+ ++T+ EH + L Q+LA G + +
Sbjct: 65 ASAGQIVWTSGATESNNLALKGVAQALDAPGHLITSRLEHKAVLDTVQQLADD-GWSVTW 123
Query: 135 IEGDDEGNLDLADAEKKIKG-AKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQ 193
+ +G + E + +LV++ V+N LG + ++ +G+ V+E GA+F VDAAQ
Sbjct: 124 LAPGTDGLIRPEQVEAAWRADTRLVSLMRVNNELGTLTDIAAIGERVRERGALFHVDAAQ 183
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
+ G + +D+ +L D ++FS HK GP GIG LY+ + + GGG
Sbjct: 184 ASGKVAIDLGRLAVDLMSFSAHK-TYGPKGIGALYVGPRAERRLKAQIHGGG 234
>sp|O51886|NIFS_BUCAP NIFS PROTEIN HOMOLOG >gi|2827032|gb|AAC38124.1| (AF008210) NifS
[Buchnera aphidicola]
Length = 404
Score = 106 bits (262), Expect = 5e-22
Identities = 69/219 (31%), Positives = 112/219 (50%), Gaps = 6/219 (2%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYL--RYRANVHRGVHRLSQMATQKYEESRKVVADFINA 75
IY D AT+ V + M Y N H+ A + + +R +++ I +
Sbjct: 5 IYLDYAATTPVEFKVAKKMMNYLTIDGIFGNPASRSHKFGWEAEEVIDIARNEISELIGS 64
Query: 76 EFDEIAFTKNTSESLNLVALGLEHLF-KKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEF 134
+ EI FT +E+ NL G+ KKG+ I+T+ EH S +L R + G + +
Sbjct: 65 DSREIVFTSGATEANNLAIKGIAFFHRKKGNHIITSKTEHKS-VLDACRYLESVGFSVTY 123
Query: 135 IEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQ 193
+ + G +DL D +K I K LV++ HV+N G+I ++ + K+ + F VDA Q
Sbjct: 124 LTPKNNGIIDLNDLKKNINKNTILVSIMHVNNETGIIQDITNISKICRSHDIFFHVDATQ 183
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKE 232
SVG + +++K L D ++FS HK GP GIG LY+ ++
Sbjct: 184 SVGKIAINLKNLFVDLMSFSAHK-IYGPKGIGGLYVRRK 221
>sp|P05344|NIFS_KLEPN NIFS PROTEIN >gi|479600|pir||S34847 nifS protein - Klebsiella
pneumoniae >gi|43880|emb|CAA31118.1| (X12600) nifS gene
product (AA 1 - 397) [Klebsiella pneumoniae]
Length = 397
Score = 104 bits (256), Expect = 2e-21
Identities = 102/397 (25%), Positives = 171/397 (42%), Gaps = 40/397 (10%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+Y DN AT+ V+EAM + + N +H A E + + A + AE+
Sbjct: 4 VYLDNNATTRLDPMVLEAMMPFLTDFYGNPS-SIHDFGIPAQAALERAHQQAAALLGAEY 62
Query: 78 -DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
EI FT + A+ L + +I+T+ EH + L + L +++G ++ I
Sbjct: 63 PSEIIFTSWPRATPRHAAIAL---LPERREIITSVVEHPATLAACEHL-ERQGYRIHRIA 118
Query: 137 GDDEGNLDLADAEKKIKG-AKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSV 195
D EG LD+A + LV+V +N GV+ + E+ ++ E+GA+F DA Q V
Sbjct: 119 VDSEGALDMAQFRAALSPRVALVSVMWANNETGVLFPIGEMAELAHEQGALFHCDAVQVV 178
Query: 196 GHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCY 255
G + + V + + D L+ S HK GP G+G LY+ + F P L GG
Sbjct: 179 GKIPIAVGQTRIDMLSCSAHKF-HGPKGVGCLYLRRG--TRFRPLLRGG----------- 224
Query: 256 KLTEPPERFEAGTPNIGGAIGLAAG--IKYIEKIGIDKIEKQERKLVKRTTEGLDELEIP 313
AGT NI G +G+ A + I G+ I + +L R + + +
Sbjct: 225 ---HQEYGRRAGTENICGIVGMGAACELANIHLPGMTHIGQLRNRLEHRLLASVPSVMVM 281
Query: 314 WYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCA------LPVMKRLKI- 366
G + G V+ + + +L++ I SG C VM+ + I
Sbjct: 282 GGGQPRV---PGTVNLAFEFIEGEAILLLLNQAGIAASSGSACTSGSLEPSHVMRAMNIP 338
Query: 367 ----NGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
+GT+R S Y +E++ + L ++ LR+
Sbjct: 339 YTAAHGTIRFSLSRYTREKEIDYVVATLPPIIDRLRA 375
>pir||D64547 iron-sulfur cofactor synthesis protein - Helicobacter pylori
(strain 26695) >gi|2313311|gb|AAD07288.1| (AE000542)
synthesis of [Fe-S] cluster (nifS) [Helicobacter pylori
26695]
Length = 387
Score = 104 bits (256), Expect = 2e-21
Identities = 86/302 (28%), Positives = 143/302 (46%), Gaps = 29/302 (9%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINA-E 76
IY DN AT+ V E MD + + N +H+ E+ + INA +
Sbjct: 5 IYLDNNATTRIDPKVKEIMDPFLRDHYGNPS-SLHQFGTETHPAIAEALDKLYKGINARD 63
Query: 77 FDEIAFTKNTSESLNLVALGL--EHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEF 134
D++ T +ES N V G+ + KKG + T H + + G+++ +
Sbjct: 64 IDDVIITSCATESNNWVLKGVYFDECLKKGKNHIVTTVAEHPAVRSTCNFLESLGVEVTY 123
Query: 135 IEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQ 193
+ ++ G++ ++ I + LV+V +N G+I +EE+G + KE+G +F DA Q
Sbjct: 124 LPINEHGSITAEQVKEAITEKTALVSVMWANNETGLIFPIEEIGAICKEKGVLFHTDAVQ 183
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELC 253
++G + VDV K ADFL+FS HK GP GIG LYI V PL GG
Sbjct: 184 AIGKIPVDVLKANADFLSFSAHKF-HGPKGIGGLYIRS---GVGLTPLFHGG-------- 231
Query: 254 CYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDE--LE 311
E +GT N+ +G+ + K+ ++ ++ E+++V + + L+E L+
Sbjct: 232 -----EHMNGRRSGTLNVPYIVGMGEAM----KLAVEHLD-YEKEVVGKLRDKLEEALLK 281
Query: 312 IP 313
IP
Sbjct: 282 IP 283
>pir||G64117 nifS protein homolog HI1343 - Haemophilus influenzae (strain Rd
KW20) >gi|1574801|gb|AAC22994.1| (U32813) H. influenzae
predicted coding region HI1343 [Haemophilus influenzae
Rd]
Length = 238
Score = 102 bits (251), Expect = 9e-21
Identities = 63/176 (35%), Positives = 95/176 (53%), Gaps = 9/176 (5%)
Query: 116 SNLLPWQRLAKKKGLKLEFIEGDDEGNLD----LADAEKKIKGAKLVAVQHVSNALGVIH 171
+N + W AKK G K+ + D +D ++ +K KLVA+ VSN G
Sbjct: 44 ANFVTWHETAKKCGAKIRVLPILDNWLIDENVLISTLSEK---TKLVALNFVSNVTGTEQ 100
Query: 172 EVEELGKMVKEEG-AIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYIN 230
++ L +++++ A+ +VDAAQ++ H+++D++ L ADFLAFS HK GP G+GVL
Sbjct: 101 PIKRLIQLIRKHSHALVLVDAAQAISHIKIDLQDLDADFLAFSAHK-IYGPNGLGVLTGK 159
Query: 231 KEFFDVFEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEK 286
+P GG +E V E P R EAGTPNI G IG A + +++K
Sbjct: 160 LTALSQLQPLFFGGKMVERVSNNRITFAELPYRLEAGTPNIAGVIGFNAVLDWLQK 215
>pir||A71960 probable nitrogenase cofactor synthesis protein nifS - Helicobacter
pylori (strain J99) >gi|4154727|gb|AAD05789.1|
(AE001459) putative AMINOTRANSFERASE [Helicobacter
pylori J99]
Length = 387
Score = 101 bits (250), Expect = 1e-20
Identities = 85/302 (28%), Positives = 142/302 (46%), Gaps = 29/302 (9%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINA-E 76
IY DN AT+ V E MD + + N +H+ E+ + INA +
Sbjct: 5 IYLDNNATTRIDPKVKEIMDPFLRDHYGNPS-SLHQFGTETHPAIAEALDKLYKGINARD 63
Query: 77 FDEIAFTKNTSESLNLVALGL--EHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEF 134
D++ T +ES N V G+ + KKG + T H + + G+++ +
Sbjct: 64 IDDVIITSCATESNNWVLKGVYFDECLKKGKNHIITTVAEHPAVRSTCNFLESLGVEVTY 123
Query: 135 IEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQ 193
+ ++ G++ ++ I + LV+V +N G+I +EE+G + KE+G +F DA Q
Sbjct: 124 LPINEHGSITADQVKEAITEKTALVSVMWANNETGLIFPIEEIGAICKEKGVLFHTDAVQ 183
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELC 253
++G + VDV K DFL+FS HK GP GIG LYI V PL GG
Sbjct: 184 AIGKIPVDVLKANVDFLSFSAHKF-HGPKGIGGLYIRS---GVGLTPLFHGG-------- 231
Query: 254 CYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDE--LE 311
E +GT N+ +G+ + K+ ++ ++ E+++V + + L+E L+
Sbjct: 232 -----EHMNGRRSGTLNVPYIVGMGEAM----KLAVEHLD-YEKEVVGKLRDKLEEALLK 281
Query: 312 IP 313
IP
Sbjct: 282 IP 283
>emb|CAB96039.1| (AL360055) putative aminotransferase [Streptomyces coelicolor
A3(2)]
Length = 400
Score = 100 bits (247), Expect = 3e-20
Identities = 109/410 (26%), Positives = 170/410 (40%), Gaps = 43/410 (10%)
Query: 7 VRKDIP-LTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEES 65
+R +P L FD + TP+PVI+A+ AN R + A + +E+
Sbjct: 8 IRAHVPALKSGTARFDAPGGTQTPQPVIDAIAAALTAPLANRGRNTEG-ERNADRIVDEA 66
Query: 66 RKVVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLA 125
R +AD + + F ++ ++ ++ L + GD++V T +H SN+ PW + A
Sbjct: 67 RSALADLLGTTPGTVVFGRSATQLAYDLSRTLAKGWGPGDEVVVTRLDHDSNIRPWVQAA 126
Query: 126 KKKGLKLEFIEGDDEGNLDLADAEKKIKG--AKLVAVQHVSNALGVIHEVEELGKMVKEE 183
+ G + + + D + + G +LVAV SN +G + ++ L +V
Sbjct: 127 EAAGADVRWADFDPATGELRPEHLAAVLGPRTRLVAVTAASNLIGTMPDLPALASLVHAN 186
Query: 184 GAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPP--L 241
GA F VDA H VD+ AD L S +K +GP +GVL E + P L
Sbjct: 187 GAHFHVDAVHYASHAVVDLAATGADTLVCSPYKF-LGP-HLGVLTGRAELLESLRPDKLL 244
Query: 242 IGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGID----------- 290
T+ PERFE GT G +A + ++ + +
Sbjct: 245 PSADTV-------------PERFELGTLPYELLAGTSAAVDFLAGLEAEAEGSRRDRLVA 291
Query: 291 ---KIEKQERKLVKRTTEGLDEL-EIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEH 346
+E E L +R GL EL I Y + + F V L P DV L E
Sbjct: 292 SFAALEAHEEALRERLERGLAELGGITVYS--RAARRTPTLLFTVAGLRPADVYRQLAER 349
Query: 347 KIMVRSGHHCALPVMKRLKI--NGTVRASFHVYNSLEEVE---TFLGVLE 391
+ +G A+ +RL + G VR Y+S E+V+ T LG LE
Sbjct: 350 AVDAPAGSFYAVEAARRLGLGDEGGVRVGLAPYSSAEDVDRLLTALGALE 399
>pir||A71107 hypothetical protein PH0626 - Pyrococcus horikoshii
>gi|3257032|dbj|BAA29715.1| (AP000003) 385aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 385
Score = 99.5 bits (244), Expect = 6e-20
Identities = 97/390 (24%), Positives = 167/390 (41%), Gaps = 32/390 (8%)
Query: 19 YFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEFD 78
Y + L P V+E + E++L A G++ + M T E + K A +
Sbjct: 14 YLNTAGLGLLPLNVLEDIAEFFLSMLA-YKDGINAVEVMDTIYLEPTLKEAAKLMKTSHR 72
Query: 79 EIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEGD 138
I + T++ L L L+ K+G IV+ E + +K G+K++ I+ +
Sbjct: 73 NITLSLQTTDGLKRALLALKP--KRGMNIVSFDLEFPTISAIVNSYSKLTGIKVKVIKNN 130
Query: 139 DEGNLDLADAEKKIKGAKLVAV-QHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVGH 197
G DL+D EK + V V G +E+ ++ E GA +VDA QS+G
Sbjct: 131 G-GIYDLSDVEKAVDDNTFAVVFSDVQWITGQRMPTKEIAEIAHEHGAWVIVDAVQSLGA 189
Query: 198 MEVDVKKLKADFLAFSGHKGPMGPT-GIGVLYINKEFFDV---------FEPPLIGGGTI 247
+EV +K+ D L G K + P G GV++++ F + EPP+
Sbjct: 190 LEVYPEKMGVDILVAGGEKWLLNPNMGSGVMFLSNRFIEESKPVLGLLNTEPPVPWSEWW 249
Query: 248 EDVELCCYKL---TEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTT 304
D + +++ + + + GTP A+ L A ++ I +GI KIE+ +L +R
Sbjct: 250 GDKDKDLWEMLPVRKDARKLDPGTPPYLAAVALKASLELINDVGIKKIERNNLRLAERVK 309
Query: 305 EGLDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMKRL 364
E +E G + L V+ + + + L E KI+V +
Sbjct: 310 EWANE-----KGFKTLGSSQIVLIMGLDFEEEKKIVNKLKEDKIIVS---------QRGA 355
Query: 365 KINGTVRASFHVYNSLEEVETFLGVLEELV 394
K +R S H YN+ +++E F +E L+
Sbjct: 356 KGIHGIRVSPHFYNNKDDIEKFAEGMESLL 385
>gb|AAC16992.1| (AF067211) similar to aminotransferases class-V; Pfam domain:
PF00266 (aminotran_5), Score=389.1, E-value=1.4e-113,
N=1 [Caenorhabditis elegans]
Length = 446
Score = 98.7 bits (242), Expect = 1e-19
Identities = 73/271 (26%), Positives = 122/271 (44%), Gaps = 39/271 (14%)
Query: 12 PLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVAD 71
P + + IY D AT+ V++AM Y + N H H A + E++R+ VA+
Sbjct: 7 PGSPQPIYLDVQATAPMDPRVVDAMLPYMINDFGNPHSRTHSYGWKAEEGVEQAREHVAN 66
Query: 72 FINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDK--IVTTPYEHHSNLLPWQRLAKKKG 129
I A+ +I FT +ES NL G+ K+ K I+T EH +L R + +G
Sbjct: 67 LIKADPRDIIFTSGATESNNLAIKGVAKFRKQSGKNHIITLQTEHKC-VLDSCRYLENEG 125
Query: 130 LKLEFIEGDDEGNLDLADAEKKIKGAK-LVAVQHVSNALGVIHEVEELG----------- 177
K+ ++ D G +D+ + I LV++ V+N +GV+ ++++G
Sbjct: 126 FKVTYLPVDKGGMVDMEQLTQSITAETCLVSIMFVNNEIGVMQPIKQIGLFFFSNQRNTP 185
Query: 178 -----------------------KMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSG 214
++ + +G F DAAQ+ G + +DV ++K D ++ S
Sbjct: 186 KKKLNLTKISKFFSFDSLIAEFSELCRSKGVYFHTDAAQATGKVPIDVNEMKIDLMSISA 245
Query: 215 HKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
HK GP G G LY+ + E + GGG
Sbjct: 246 HK-IYGPKGAGALYVRRRPRVRIEAQMSGGG 275
>sp|Q03046|CEFD_NOCLA ISOPENICILLIN N EPIMERASE >gi|419704|pir||S30901 isopenicillin N
epimerase (EC 5.1.1.-) - Streptomyces lactamdurans
>gi|49302|emb|CAA78377.1| (Z13984) CefD [Streptomyces
lactamdurans] >gi|445071|prf||1908385A isopenicillin N
epimerase [Streptomyces lactamdurans]
Length = 398
Score = 98.3 bits (241), Expect = 1e-19
Identities = 99/412 (24%), Positives = 181/412 (43%), Gaps = 43/412 (10%)
Query: 6 DVRKDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRG-VHRLSQMATQKYEE 64
+ R+ + L V + + P+PV + + RA + G + LS+
Sbjct: 8 NAREQVSLDPAVTNLNAGSCGPLPRPVFDRVT-----VRARMAAGPMDFLSRQLPPLLWT 62
Query: 65 SRKVVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRL 124
+R+ +A ++ A + +AF N + ++NLVA ++ G +I+ + E+ W+R+
Sbjct: 63 ARERLAGYLGARPERLAFATNVTGAVNLVASSVQPHLAAGGEILLSDQEYAPMRWCWERV 122
Query: 125 AKKKGLKLEFIE------GDDEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEVEELGK 178
A+ +GL + G + ++ A A + +L+ HV ++ G++ L +
Sbjct: 123 ARHQGLVVRTFRLPVQPLGSPDEVVEAATAAMGPR-TRLLFFSHVVSSTGLVLPATRLCE 181
Query: 179 MVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFE 238
+ G + VVD AQ+ G ++D+ L D+ A SGHK + PTG+G L+ ++ V
Sbjct: 182 EARRRGVLTVVDGAQAPGFTDLDLAALPCDYYAGSGHKWLLAPTGVGFLHFAEDQGGVLR 241
Query: 239 PPLIGGGTIEDVELCCYKLTEPPERFEA----------GTPNIGGAIGLAAGIKYIEKIG 288
PP + G D E ++ RF + GT ++ + + + I + +G
Sbjct: 242 PPQVSWGYRPDGE----NPSDERNRFGSTDRLRNLECEGTRDLCPWLAVPSAIDFQAGLG 297
Query: 289 IDKIEKQERKLVKRTTEGLDELEIPWYG-----PRNLDKHAGVVSFNVPPLHPHDVASVL 343
++ ++ R+L T E L W G P + + +F +PP D A +
Sbjct: 298 HGRVRERMRELAAFTRERLS----GWRGLEPVTPAHPGLSGAMTAFRLPP--GTDTAGL- 350
Query: 344 DEHKIMVRSGHHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVK 395
H + R +PV+ R +R S H YN+ EVE L+EL K
Sbjct: 351 -RHGLWDR--FRIDVPVLDR-PDGPLLRVSTHFYNTETEVERLAEALKELSK 398
>pir||S37296 nifS protein - Klebsiella pneumoniae >gi|149344|gb|AAA25156.1|
(M17350) nifS-encoded protein [Klebsiella pneumoniae]
Length = 397
Score = 98.3 bits (241), Expect = 1e-19
Identities = 100/397 (25%), Positives = 169/397 (42%), Gaps = 40/397 (10%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+Y DN AT+ V+EAM + + N +H A E + + A + AE+
Sbjct: 4 VYLDNNATTRLDPMVLEAMMPFLTDFYGNPS-SIHDFGIPAQAALERAHQQAAALLGAEY 62
Query: 78 -DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
EI FT + A+ L + +I+T+ EH + L + L +++G ++ I
Sbjct: 63 PSEIIFTSWPRATPRHAAIAL---LPERREIITSVVEHPATLAACEHL-ERQGYRIHRIA 118
Query: 137 GDDEGNLDLADAEKKIKG-AKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSV 195
D EG LD+A + LV+V +N GV+ + E+ ++ E+GA+F DA Q V
Sbjct: 119 VDSEGALDMAQFRAALSPRVALVSVMWANNETGVLFPIGEMAELAHEQGALFHCDAVQVV 178
Query: 196 GHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCY 255
G + + V + + L+ S HK P G+G LY+ + F P L GG
Sbjct: 179 GKIPIAVGQTRIHMLSCSAHKF-HPPKGVGCLYLRRG--TRFRPLLRGG----------- 224
Query: 256 KLTEPPERFEAGTPNIGGAIGLAAG--IKYIEKIGIDKIEKQERKLVKRTTEGLDELEIP 313
AGT NI G +G+ A + I G+ I + +L R + + +
Sbjct: 225 ---HQEYGRRAGTENICGIVGMGAACELANIHLPGMTHIGQLRNRLEHRLLASVPSVMVM 281
Query: 314 WYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCA------LPVMKRLKI- 366
G + G V+ + + +L++ I SG C VM+ + I
Sbjct: 282 GGGQPRV---PGTVNLAFEFIEGEAILLLLNQAGIAASSGSACTSGSLEPSHVMRAMNIP 338
Query: 367 ----NGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
+GT+R S Y +E++ + L ++ LR+
Sbjct: 339 YTAAHGTIRFSLSRYTREKEIDYVVATLPPIIDRLRA 375
>dbj|BAB06923.1| (AP001518) L-cysteine sulfurtransferase (iron-sulfur cofactor
synthesis) [Bacillus halodurans]
Length = 380
Score = 97.5 bits (239), Expect = 2e-19
Identities = 100/403 (24%), Positives = 180/403 (43%), Gaps = 50/403 (12%)
Query: 17 VIYFDNTATSLTPKPVIEAMDEYY---LRYRANVHRGVHRLSQMATQKYEESRKVVADFI 73
+IY DN+AT+ +P +E +D Y L + N +HR+ A Q ++++ +A +
Sbjct: 1 MIYLDNSATT---RPFMEVVDSYTKASLTFFGNPS-SLHRMGTEAEQLLLKTKEQIAGIL 56
Query: 74 NAEFDEIAFTKNTSESLNLVALGLEHLFK-KGDKIVTTPYEHHSNLLPWQRLAKKKGLKL 132
EI +T +E NL G + K +G ++TT EH S + ++ L + +G K+
Sbjct: 57 GISSSEIIYTSGGTEGNNLAIKGTAYQLKGRGTHLITTAVEHASTIEAFRSL-EAEGFKV 115
Query: 133 EFIEGDDEGNLDLADAEKKIKGAK-LVAVQHVSNALGVIHEVEELGKMVKEEGAI-FVVD 190
+++ G + + + LV++ HV+N G+I VE +G+++K + F +D
Sbjct: 116 TYLKPGPTGQVTAEQVKAALTNQTILVSIIHVNNETGIIMPVEGIGELLKGYPKVRFHID 175
Query: 191 AAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDV 250
Q + + + D + FS HK G G G +Y K+ + PL GG E
Sbjct: 176 HVQGATKVPLRLAHGLIDLVTFSSHKF-HGLKGTGFIY-KKDGLRL--TPLFHGGGQEGA 231
Query: 251 ELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLV---KRTTEGL 307
+GT N+ GA+ A + ++ ++ E++ KL+ + EGL
Sbjct: 232 -------------LRSGTENVAGAVATAKAL----RMAMELYEERHDKLLHLRNQLLEGL 274
Query: 308 DELE-IPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHC---------- 356
E+E + P+ L +V+F++P + P + L + I V + C
Sbjct: 275 SEIEGVVINTPKEL-AAPHIVNFSIPKMKPEVIIQALSKRDIHVSTKSACSTKRSEPSHV 333
Query: 357 --ALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSL 397
A+ V K + N +R S + +EV L L+ L+ L
Sbjct: 334 LLAMGVGKE-RANSAIRLSLSYLTTEDEVSKALQELQHLIPEL 375
>gb|AAC33372.1| (AF001780) NifS [Cyanothece PCC8801]
Length = 400
Score = 96.7 bits (237), Expect = 4e-19
Identities = 102/400 (25%), Positives = 178/400 (44%), Gaps = 39/400 (9%)
Query: 15 QEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFIN 74
++ IY DN AT+ + V+ AM Y Y N +H +R+ VA +
Sbjct: 2 KDCIYLDNNATTQVDEEVLGAMLPYLTLYYGNPS-SMHTFGGQVGSAIRTAREQVAALLG 60
Query: 75 AEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEF 134
AE EI FT +E N I+TT EH + L + L +++G + +
Sbjct: 61 AEPSEIVFTSCGTEGDNAAIRAALAAQPNKRHIITTEVEHPAILNLCKNL-ERQGYTVTY 119
Query: 135 IEGDDEGNLDLADAEKKIKG-AKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQ 193
+ +++G LDL++ E + G +V++ + +N GVI VE++G+M KE GA+F VDA Q
Sbjct: 120 LSVNNQGQLDLSELEASLTGNTAVVSIMYANNETGVIFPVEQVGQMAKEYGALFHVDAVQ 179
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELC 253
+VG + +++ + D L + K M P G+ ++I + F P LIGG
Sbjct: 180 AVGKVPLNMAESTIDMLTYPAIKF-MPPKGL--VHICRR-NTRFRPLLIGG--------- 226
Query: 254 CYKLTEPPERFEAGTPNIGG--AIGLAAGI-KYIEKIGIDKIEKQERKLVKRTTEGLDEL 310
AGT N+ G A+G AA + Y + G E++ R +++T +
Sbjct: 227 -----HQERGRRAGTENVPGIVALGKAAELAAYHLQYGTS--ERELRDYLEQTILTIIPD 279
Query: 311 EIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMKRLKI---- 366
+ P + + F + + L+++ I SG C ++ I
Sbjct: 280 TVLNGHPVQRLPNTSNIGFKF--IEGEAILLSLNQYGICASSGSACTSGSLEPSHILRAM 337
Query: 367 -------NGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
+G++R S + + E+++ L VL ++ LR+
Sbjct: 338 GLPYSVLHGSIRFSLSRFTTQEQIQKVLEVLPGIIDRLRA 377
>pir||F69666 iron-sulfur cofactor synthesis protein nifZ - Bacillus subtilis
>gi|2293229|gb|AAC00307.1| (AF008220) NifS2 [Bacillus
subtilis] >gi|2635443|emb|CAB14937.1| (Z99119) NifS
protein homolog [Bacillus subtilis]
Length = 381
Score = 96.7 bits (237), Expect = 4e-19
Identities = 91/399 (22%), Positives = 170/399 (41%), Gaps = 39/399 (9%)
Query: 17 VIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAE 76
+IY DN++T+ V+ ++ RY N +HR Q + ++ + + +
Sbjct: 1 MIYLDNSSTTKPYDEVLNVYEQTSSRYFGNPS-SLHRYGAETEQLLQAAKNQIKRSLGLK 59
Query: 77 FDEIAFTKNTSESLNLVALGLE-HLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFI 135
+I FT +E+ N G K G I+ T EH S ++L + G + ++
Sbjct: 60 KYDIVFTSGATEANNAALKGAALSKIKTGKHIIATSIEHPSVTESLEQLKELFGFDITYL 119
Query: 136 EGDDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAI-FVVDAAQ 193
+++G + + D + I+ LV++ HV+N +G + +E G+++KE I F VD Q
Sbjct: 120 SVNEDGFVSIEDLKAAIRPDTVLVSMMHVNNEVGSVQPIEAAGEVLKEHSNILFHVDYVQ 179
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELC 253
+ + + ++K D + SGHK G G G L + + + PLI GG+ +
Sbjct: 180 GIYKVPLAIEKAGIDLCSISGHKF-HGLKGTGALIVKEGTRLI---PLITGGSQQ----- 230
Query: 254 CYKLTEPPERFEAGTPNIGGAIGLAAGIKYIE---KIGIDKIEKQERKLVKRTTEGLDEL 310
+ AGT + GA+ LA I +D + + + R L E+
Sbjct: 231 --------KGIRAGTEHTAGAVSLAKAINLASADFDTRLDTMTAVKELFMNR----LSEI 278
Query: 311 EIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMKRLKI---- 366
E ++ +++F+VP + + +L+E I V + C+ K K+
Sbjct: 279 EGVVINTPQMNSAPHIINFSVPGIKAEVLLHMLEEQDIFVSTTSACSAKEHKPSKVLLEM 338
Query: 367 -------NGTVRASFHVYNSLEEVETFLGVLEELVKSLR 398
++R S + + + E F+ L +K LR
Sbjct: 339 GKGEQIAGSSIRISLNYSQTSDVAEPFMNALRPGIKKLR 377
>sp|Q01179|NIFS_RHOSH NIFS PROTEIN >gi|282176|pir||C41880 nitrogenase cofactor synthesis
protein nifS - Rhodobacter sphaeroides
>gi|151968|gb|AAA26137.1| (M86823) nifS [Rhodobacter
sphaeroides]
Length = 387
Score = 96.0 bits (235), Expect = 7e-19
Identities = 102/401 (25%), Positives = 168/401 (41%), Gaps = 42/401 (10%)
Query: 16 EVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINA 75
E +Y DN AT+ ++AM + N +H + + +R+ V + I A
Sbjct: 2 ERVYLDNNATTRLAPEALQAMLPFLTEEFGNPS-SLHGQGRAPARALMAARRAVLELIGA 60
Query: 76 EFD-EIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEF 134
E D EI FT +E+ +IVT+ EH + L L +++G+ +
Sbjct: 61 EADSEILFTSGGTEADTTAIRSALAADPSRREIVTSTVEHAAVLALCDHLERQEGVTVHR 120
Query: 135 IEGDDEGNLDLADAEKKIKG-AKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQ 193
I D +G LD+ + LV++ +N G + VE L ++ GA+F DA Q
Sbjct: 121 IPVDGDGRLDIEAYRAALSPRVALVSLMWANNETGTVFPVEGLAELAHRAGALFHTDAVQ 180
Query: 194 SVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELC 253
+VG + + ++ + D L+ S HK GP G+G L++ K V PLI GG
Sbjct: 181 AVGKVPIVLRGTEIDMLSLSAHKF-HGPKGVGALWLRK---GVPFQPLIRGG-------- 228
Query: 254 CYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELEIP 313
AGT NI G +GL ++ + R L R +G+ +P
Sbjct: 229 -----RQQRGHRAGTENIPGIVGLGRA----AELALGGDHGAVRLLRDRLEQGI-LARVP 278
Query: 314 ----WYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCA------LPVMKR 363
P + + V+F+ + +LD I V SG CA V++
Sbjct: 279 KARVLGDPLDRLPNTSCVAFDF--AEGEAIVMLLDRAGICVSSGAACASGAMEPSHVIRA 336
Query: 364 LKI-----NGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
+K+ +G +R S + + E++ L VL +V LR+
Sbjct: 337 MKVPFTAAHGAIRFSLSHWTTAAEIDRLLEVLPPIVDQLRA 377
>pir||D70858 probable iron-sulfur cofactor synthesis protein Rv3025c -
Mycobacterium tuberculosis (strain H37RV)
>gi|2791623|emb|CAA16110.1| (AL021287) hypothetical
protein Rv3025c [Mycobacterium tuberculosis]
Length = 393
Score = 95.6 bits (234), Expect = 9e-19
Identities = 93/351 (26%), Positives = 158/351 (44%), Gaps = 29/351 (8%)
Query: 19 YFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEFD 78
Y D+ AT+ IEAM R N +H + A ++ EE+R+++AD + A
Sbjct: 3 YLDHAATTPMHPAAIEAMAAVQ-RTIGNAS-SLHTSGRSARRRIEEARELIADKLGARPS 60
Query: 79 EIAFTKNTSESLNLVALGLEHLFKKGD----KIVTTPYEHHSNLLPWQRLAKKKGLKLEF 134
E+ FT +ES NL G+ + + +IVTT EHH+ L L + +G + +
Sbjct: 61 EVIFTAGGTESDNLAVKGIYWARRDAEPHRRRIVTTEVEHHAVLDSVNWLVEHEGAHVTW 120
Query: 135 IEGDDEGNLD---LADAEKKIKGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDA 191
+ +G++ L +A + LV+V +N +G I + E+ + E G DA
Sbjct: 121 LPTAADGSVSATALREALQSHDDVALVSVMWANNEVGTILPIAEMSVVAMEFGVPMHSDA 180
Query: 192 AQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVE 251
Q+VG + +D ++ +GHK GP G+G L + ++ V P + GGG D+
Sbjct: 181 IQAVGQLPLDFGASGLSAMSVAGHKFG-GPPGVGALLLRRDVTCV--PLMHGGGQERDI- 236
Query: 252 LCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEG-LDEL 310
+GTP++ A+G+A + I G+++ + R L R EG L E+
Sbjct: 237 -------------RSGTPDVASAVGMATAAQ-IAVDGLEENSARLRLLRDRLVEGVLAEI 282
Query: 311 -EIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPV 360
++ G + + AG F + +LD + I +G C V
Sbjct: 283 DDVCLNGADDPMRLAGNAHFTFRGCEGDALLMLLDANGIECSTGSACTAGV 333
>sp|Q52069|NIFS_ENTAG NIFS PROTEIN >gi|1480127|emb|CAA68020.1| (X99694) nifS
[Enterobacter agglomerans]
Length = 401
Score = 95.2 bits (233), Expect = 1e-18
Identities = 97/395 (24%), Positives = 163/395 (40%), Gaps = 33/395 (8%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+Y DN AT+ V+EAM Y Y N +H E +R+ VA + A +
Sbjct: 4 VYLDNNATTRIDPMVLEAMMPYLRDYYGNPS-SIHDFGGPCRAGLECAREQVASLLGAAY 62
Query: 78 -DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
EI FT +E+ + L + +I+TT EH + L + L +++G + I
Sbjct: 63 TSEIIFTSCATEATSTAIYSAIALAPERREIITTAVEHPATLAVCEHL-ERQGYMIHRIG 121
Query: 137 GDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSV 195
+EG LD A + + LV + +N GV+ V E+ E G +F DA Q+V
Sbjct: 122 VSEEGALDTAHYYDALSENVALVTMMWANNETGVLFPVSEMATAAHERGILFHCDAVQAV 181
Query: 196 GHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCY 255
G + + ++ D L+ S HK GP G G LY+ + + PL+ GG E
Sbjct: 182 GKIPISLRATDIDMLSCSAHK-IHGPKGCGCLYLRR---NTRFRPLVRGGHQE------- 230
Query: 256 KLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELEIPWY 315
AGT NI G +G+ A + + + + + + + T L +
Sbjct: 231 ------RGRRAGTENIAGIVGMGAACE-LADVHMPMMASVQEMRDRLETGLLTTIPHTLL 283
Query: 316 GPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCA------LPVMKRLKI--- 366
N + V+ + + +L+ I SG C VM+ + I
Sbjct: 284 MGANQPRTPNTVNIAFEYIEGEAILLLLNHCGIAASSGSACTSGSLEPSHVMRAMNIPYT 343
Query: 367 --NGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
+G++R S + E+E + + ++V LR+
Sbjct: 344 AAHGSIRFSLSRFTREREIEWAIEQMPDIVARLRT 378
>pir||E70390 iron-sulfur cofactor synthesis protein nifS - Aquifex aeolicus
>gi|2983536|gb|AAC07111.1| (AE000720) FeS cluster
formation protein NifS [Aquifex aeolicus]
Length = 370
Score = 94.8 bits (232), Expect = 2e-18
Identities = 98/398 (24%), Positives = 182/398 (45%), Gaps = 49/398 (12%)
Query: 17 VIYFDNTATSLTPKPVIEAMDEYYLRYRANVH---RGVHRLSQMATQKYEESRKVVADFI 73
+IYFDN AT+ PV+ + + + +++ VH L + A + E +RK +A+++
Sbjct: 1 MIYFDNAATT----PVLPEVKNFLCK-ALDIYGNPSSVHTLGKFARSEIETARKTIANYL 55
Query: 74 NAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLE 133
+ + I F +E N + + + +G+ +V + EH S + + K +++
Sbjct: 56 KVKPENIIFNSCATEGNNTIIRTILNNKDRGN-VVLSAIEHKS-VKEAIKFWANKSIEIR 113
Query: 134 FIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAA 192
I+ + G +DL I LV V +VSN G I ++E+ K+ ++G + DA
Sbjct: 114 EIKVNKNGVIDLEHLYHLIDSNTILVCVMYVSNEFGTIQPIQEIAKICSDKGVPLLTDAV 173
Query: 193 QSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVEL 252
Q++G + +++K + + FSGHK G G LYI+ E +EP ++GGG
Sbjct: 174 QAIGKIPIELKNI--SYATFSGHKF-HAIKGSGFLYISDE--ANYEPLIVGGG------- 221
Query: 253 CCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKL----VKRTTEGLD 308
E +R +GT N+ G + LA ++ I + ++Q +KL E L
Sbjct: 222 -----QENGKR--SGTENVVGILSLAKALEIIVS-NFSRYQEQLKKLRDLFENLLLEALP 273
Query: 309 ELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCAL----PVMKRL 364
+ +I ++ ++ + S +P ++ + L E I +G C P L
Sbjct: 274 DAQIV---GKDAERSPSISSVIMPKFFGAEIVNKLSEKGIYCSTGSACLSGEYEPNKHML 330
Query: 365 KINGT-------VRASFHVYNSLEEVETFLGVLEELVK 395
K+ + VR SF + N EEV + ++E+ +
Sbjct: 331 KMGFSQEKALRMVRFSFGLLNKEEEVIECIERIKEIYR 368
>gb|AAA22168.1| (M17349) nifS protein [Azotobacter vinelandii]
Length = 402
Score = 94.0 bits (230), Expect = 3e-18
Identities = 100/399 (25%), Positives = 165/399 (41%), Gaps = 40/399 (10%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+Y DN AT+ +++AM ++ N +H L +++R+ V + AE
Sbjct: 4 VYLDNNATTRVDDEIVQAMLPFFTEQFGNPS-SLHSLGNQVGMALKKARQSVQKLLGAEH 62
Query: 78 D-EIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
D EI FT +ES + L + ++TT EH + L LA + G + +
Sbjct: 63 DSEILFTSCGTESDSTAILSALKAQPERKTVITTVVEHPAVLSLCDYLASE-GYTVHKLP 121
Query: 137 GDDEGNLDLAD-AEKKIKGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSV 195
D +G LDL A +V+V +N G + +EE+ ++ + G +F DA Q+V
Sbjct: 122 VDKKGRLDLEHYASLLTDDVAVVSVMWANNETGTLFPIEEMARLADDAGIMFHTDAVQAV 181
Query: 196 GHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCY 255
G + +D+K L+ GHK P G+GVLY+ + F P L GG
Sbjct: 182 GKVPIDLKNSSIHMLSLCGHK-LHAPKGVGVLYLRRG--TRFRPLLRGG----------- 227
Query: 256 KLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELEIPWY 315
AGT N IGL G+ + + E E ++ E +P
Sbjct: 228 ---HQERGRRAGTENAASIIGL--GVAAERALQFMEHENTEVNALRDKLEAGILAVVPHA 282
Query: 316 ----GPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCA------LPVMKRLK 365
P N + ++F + + +L++ I SG C VM+ +
Sbjct: 283 FVTGDPDNRLPNTANIAFEY--IEGEAILLLLNKVGIAASSGSACTSGSLEPSHVMRAMD 340
Query: 366 I-----NGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
I +GTVR S Y + EE++ + + +V LR+
Sbjct: 341 IPYTAAHGTVRFSLSRYTTEEEIDRVIREVPPIVAQLRN 379
>pir||T45399 iron-sulfur cofactor synthesis protein MLCB637.06 [similarity] -
Mycobacterium leprae >gi|2414532|emb|CAB16421.1|
(Z99263) nifS-like protein [Mycobacterium leprae]
Length = 396
Score = 93.6 bits (229), Expect = 4e-18
Identities = 104/402 (25%), Positives = 177/402 (43%), Gaps = 44/402 (10%)
Query: 19 YFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEFD 78
Y D+ AT+ IEAM L N +H ++A ++ EESR+++AD + A
Sbjct: 3 YLDHAATTPMHPAAIEAMTTA-LGTVGNAS-SLHTSGRVARRRIEESRELIADKLAARPS 60
Query: 79 EIAFTKNTSESLNLVALGLEHLFKKGD----KIVTTPYEHHSNLLPWQRLAKKKGLKLEF 134
E+ FT +ES NL G+ + +IVTT EHH+ L L + +G ++ +
Sbjct: 61 EVIFTAGGTESDNLAVKGIYWARRDAQPCHRRIVTTEVEHHAVLDSVNWLVEHEGAEVTW 120
Query: 135 IEGDDEGNLD---LADAEKKIKGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDA 191
+ +G++ L +A ++ LV+V +N +G + V EL + E G DA
Sbjct: 121 LPTAADGSVSAFGLREALQQHNDVALVSVMWANNEVGTVMPVVELAAVAAEFGVPMHSDA 180
Query: 192 AQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVE 251
Q+VG + V ++ + HK GP G+G L + ++ V P L GGG D+
Sbjct: 181 IQAVGQLPVKFGVSGLSAMSVAAHKFG-GPPGVGALLLRRDISCV--PLLHGGGQERDI- 236
Query: 252 LCCYKLTEPPERFEAGTPNIGGAIGLAAGIKY-IEKIGIDKIEKQ--ERKLVKRTTEGLD 308
+GTP + +G+AA + ++ + I+ I Q +L++ +D
Sbjct: 237 -------------RSGTPGVASVVGMAAAAQIAVDGLEINSIRLQVLRDRLIEGVLATID 283
Query: 309 ELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMKRLKI-- 366
++ + G R+ + G F + +LD + I +G C V + +
Sbjct: 284 DVAL--NGARDRMRLPGNAHFIFRGCEGDVLLMLLDANGIECSTGSACTSGVPQPSHVLI 341
Query: 367 ---------NGTVRASFHVYNSLE-EVETFLGVLEELVKSLR 398
G++R S +NS+E +VE L VL V R
Sbjct: 342 AMGADPASARGSLRLSLG-HNSVEVDVEEVLRVLPGAVDRAR 382
>pir||E75054 capreomycin acetyltransferase or nifs-like protein PAB0943 -
Pyrococcus abyssi (strain Orsay)
>gi|5458843|emb|CAB50330.1| (AJ248287) CAPREOMYCIN
ACETYLTRANSFERASE or NIFS-LIKE PROTEIN [Pyrococcus
abyssi]
Length = 386
Score = 93.2 bits (228), Expect = 5e-18
Identities = 95/392 (24%), Positives = 162/392 (41%), Gaps = 35/392 (8%)
Query: 19 YFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEFD 78
Y + L P V++ + E+ L G++ + + E A +
Sbjct: 14 YLNTAGLGLLPITVLKEVTEFLLDV-IKYKEGINAVEILDPMYLEPVLSEAAKLMRTRSR 72
Query: 79 EIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEGD 138
+ + T++ L L LE KK KI++ E + + A+K LK+E IE
Sbjct: 73 NVGLSIQTTDGLKRALLALEP--KKNMKIISFDLEFPTISAVVKSYAEKHNLKVEVIESK 130
Query: 139 DEGNLDLADAEKKIKGAKLVAV-QHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVGH 197
+ G D++D EK I V V G +E+ ++ E GA +VDA QS+G
Sbjct: 131 N-GLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHEHGAWVIVDAVQSLGA 189
Query: 198 MEVDVKKLKADFLAFSGHKGPMGPT-GIGVLYINKEFFDV---------FEPPLIGGGTI 247
++V +KL D L G K + P G G++Y++ F + EPP+
Sbjct: 190 LQVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKPIIGLLNTEPPVPWSDWW 249
Query: 248 EDVELCCYKLTE---PPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTT 304
D + + + + + GTP A+ L ++ I +GI KIEK++ KL +
Sbjct: 250 GDKDKDLWDILPVRGDARKLDPGTPAYLSAVALKPSLELINDVGIKKIEKEDLKLAEMVR 309
Query: 305 EGLDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMKR- 363
E E G + + F + K +VR + + V +R
Sbjct: 310 EWALEKGFNVLGDSQITLIVTGIEF--------------EREKEIVRKLNEMGIIVSQRG 355
Query: 364 -LKINGTVRASFHVYNSLEEVETFLGVLEELV 394
I+G +R S H+YN+ E++E F+ +E +
Sbjct: 356 AKGIHG-IRVSPHLYNTKEDIEKFIEGIERFL 386
>sp|P05341|NIFS_AZOVI NIFS PROTEIN >gi|420859|pir||S29757 nitrogenase cofactor synthesis
protein nifS - Azotobacter vinelandii
>gi|142361|gb|AAA64726.1| (M20568) nifS protein
[Azotobacter vinelandii]
Length = 402
Score = 92.4 bits (226), Expect = 8e-18
Identities = 99/399 (24%), Positives = 164/399 (40%), Gaps = 40/399 (10%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
+Y DN AT+ +++AM ++ N +H +++R+ V + AE
Sbjct: 4 VYLDNNATTRVDDEIVQAMLPFFTEQFGNPS-SLHSFGNQVGMALKKARQSVQKLLGAEH 62
Query: 78 D-EIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
D EI FT +ES + L + ++TT EH + L LA + G + +
Sbjct: 63 DSEILFTSCGTESDSTAILSALKAQPERKTVITTVVEHPAVLSLCDYLASE-GYTVHKLP 121
Query: 137 GDDEGNLDLAD-AEKKIKGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSV 195
D +G LDL A +V+V +N G + +EE+ ++ + G +F DA Q+V
Sbjct: 122 VDKKGRLDLEHYASLLTDDVAVVSVMWANNETGTLFPIEEMARLADDAGIMFHTDAVQAV 181
Query: 196 GHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCY 255
G + +D+K L+ GHK P G+GVLY+ + F P L GG
Sbjct: 182 GKVPIDLKNSSIHMLSLCGHK-LHAPKGVGVLYLRRG--TRFRPLLRGG----------- 227
Query: 256 KLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELEIPWY 315
AGT N IGL G+ + + E E ++ E +P
Sbjct: 228 ---HQERGRRAGTENAASIIGL--GVAAERALQFMEHENTEVNALRDKLEAGILAVVPHA 282
Query: 316 ----GPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCA------LPVMKRLK 365
P N + ++F + + +L++ I SG C VM+ +
Sbjct: 283 FVTGDPDNRLPNTANIAFEY--IEGEAILLLLNKVGIAASSGSACTSGSLEPSHVMRAMD 340
Query: 366 I-----NGTVRASFHVYNSLEEVETFLGVLEELVKSLRS 399
I +GTVR S Y + EE++ + + +V LR+
Sbjct: 341 IPYTAAHGTVRFSLSRYTTEEEIDRVIREVPPIVAQLRN 379
>sp|P42253|YCBU_BACSU HYPOTHETICAL 40.8 KD PROTEIN IN PCP-LMRB INTERGENIC REGION
PRECURSOR (ORF21) >gi|7475675|pir||E69754 nifS protein
homolog ycbU - Bacillus subtilis
>gi|2415717|dbj|BAA22227.1| (AB000617) YcbU [Bacillus
subtilis] >gi|2632552|emb|CAB12060.1| (Z99105) similar
to NifS protein homolog [Bacillus subtilis]
Length = 370
Score = 90.9 bits (222), Expect = 2e-17
Identities = 80/291 (27%), Positives = 128/291 (43%), Gaps = 14/291 (4%)
Query: 3 IPEDVRKDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKY 62
+PE R+ P Q + + S +PV A+ +YY + +++G + + A +K
Sbjct: 4 LPEQYRQLFPTLQTHTMLASCSQSALAEPVSRAIQDYY---DSLLYKGTNW--KEAIEKT 58
Query: 63 EESRKVVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQ 122
E +R A I AE DE+A + S++L VA L KK +V T + + WQ
Sbjct: 59 EFARNEFAKLIGAEPDEVAIVPSVSDALVSVASYLTAFGKK--HVVYTDMDFPAVPHVWQ 116
Query: 123 RLAKKKGLKLEFIEGDDEGNLDLADAEKKIKGAK-LVAVQHVSNALGVIHEVEELGKMVK 181
+ + I+G L L E I L V HV G + +++ + ++ +
Sbjct: 117 AHSDYTVSVIPSIDGV----LPLEQYETHISDETVLTCVPHVHYRDGYVQDIKAIAEISQ 172
Query: 182 EEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPL 241
+G++ VDA QS GH+ +DVK+ D LA K +G G+ LY+ KE D +P
Sbjct: 173 RKGSLLFVDAYQSAGHIPIDVKEWGVDMLAAGTRKYLLGIPGVAFLYVRKELADALKPKA 232
Query: 242 IGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKI 292
E Y + RF+ GTP AA + + IG+ I
Sbjct: 233 SAWFGRESGFDGAY--AKVARRFQTGTPAFISVYAAAAALSLLNHIGVSHI 281
>pir||S74526 nitrogenase cofactor synthesis protein nifS - Synechocystis sp.
(strain PCC 6803) >gi|1651750|dbj|BAA16678.1| (D90899)
NifS protein [Synechocystis sp.]
Length = 391
Score = 88.2 bits (215), Expect = 2e-16
Identities = 70/267 (26%), Positives = 121/267 (45%), Gaps = 21/267 (7%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
IY D +AT+ P+ ++A + +L +H A E +R VA +NA
Sbjct: 3 IYLDYSATT-PPRQEVKAAVQSFLDQSWGNPASLHHWGNRAALALETARLQVAQLLNASH 61
Query: 78 -DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIE 136
D I FT +E+ +L G+ + ++ + EH + P R + +G ++ +
Sbjct: 62 PDSIVFTSGGTEANHLAIFGVTRNYPSPQHLIISTVEHSAIAEP-VRWLENQGWQVTRLG 120
Query: 137 GDDEGNLDLADAEKKIKG-AKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSV 195
+ +G ++ + E+ I+ L++V + + +G + +EELG++ ++ F DA Q
Sbjct: 121 VNAQGRVNPLELEQAIQSNTVLISVIYGQSEVGTLQPIEELGRIARQRHIPFHTDAVQVA 180
Query: 196 GHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCY 255
G VDVK+L D L+ S HK G G G LYI+ + P L+GGG
Sbjct: 181 GRCPVDVKRLPVDLLSLSSHK-LYGIQGAGALYIHPDL--AIGPLLMGGG---------- 227
Query: 256 KLTEPPERFEAGTPNIGGAIGLAAGIK 282
+ +GTP + +GL K
Sbjct: 228 ----QEQGLRSGTPPLPAIVGLGIAAK 250
>sp|Q07177|NIFS_RHOCA NIFS PROTEIN >gi|486694|pir||S34816 nitrogenase cofactor synthesis
protein nifS - Rhodobacter capsulatus
>gi|297918|emb|CAA48487.1| (X68444) nifS [Rhodobacter
capsulatus]
Length = 384
Score = 87.8 bits (214), Expect = 2e-16
Identities = 101/404 (25%), Positives = 164/404 (40%), Gaps = 44/404 (10%)
Query: 13 LTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADF 72
+TQ IY DN AT+ V+ AM Y+ + N G H A ++R VA
Sbjct: 1 MTQPAIYLDNNATTRVFPEVVAAMLPYFTEHFGNASSG-HGFGAQAGLGLRKARLAVAAL 59
Query: 73 INAEFD-EIAFTKNTSESLNLVALGLEHLFKKGDK-IVTTPYEHHSNLLPWQRLAKKKGL 130
I A + EI FT +E+ N A+ + G + I+ + EH + L+ L K +G+
Sbjct: 60 IGAASEQEILFTSGGTEA-NTTAIRSALAVQDGRREIIISAVEHPAILMLAADLEKTEGV 118
Query: 131 KLEFIEGDDEGNLDLADAEKKIKGAKLVA-VQHVSNALGVIHEVEELGKMVKEEGAIFVV 189
++ I D G LDLA + VA + +N + V +L GA+F
Sbjct: 119 RVHRIPVDHLGRLDLAAYRAALSDRVAVASIMWANNRYDL--PVPQLAAEAHAAGALFHT 176
Query: 190 DAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIED 249
DA Q+VG + + + + D L+ SGHK GP G+G LY+ K V PL+ GG E
Sbjct: 177 DAVQAVGKLPMSLADTEIDMLSLSGHKF-HGPKGVGALYLRK---GVAFRPLLRGGKQE- 231
Query: 250 VELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDE 309
AGT N+ +G+ + + + + + + L+
Sbjct: 232 ------------RGRRAGTENVPALVGMGLAAEIVAARMAADLSR-----IATLRDWLES 274
Query: 310 LEIPWYGPRNL----DKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMKRLK 365
+ G + L ++ A + + V + L I + +G CA M+
Sbjct: 275 GILALGGCQRLGDPANRLANTCTLAFDRIDSEAVLTKLARAGIAISTGSACASGAMEPSH 334
Query: 366 I-----------NGTVRASFHVYNSLEEVETFLGVLEELVKSLR 398
+ +G VR S + E+ L +L E++ LR
Sbjct: 335 VTRAMAVPFTAAHGVVRFSLSADTTAAELSRVLAILPEILAELR 378
>gb|AAD02935.1| (AF061964) L-cysteine/cystine lyase C-DES [Synechocystis PCC6714]
Length = 393
Score = 87.4 bits (213), Expect = 3e-16
Identities = 95/403 (23%), Positives = 166/403 (40%), Gaps = 41/403 (10%)
Query: 8 RKDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRK 67
R P YF+ + P +EA+ Y + N + +Q Q + R+
Sbjct: 11 RHQFPGLANKTYFNFGGQGILPTVALEAITAMYGYLQENGPFSI-AANQHIQQLIAQLRQ 69
Query: 68 VVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKK 127
+A+ N + + I T N + ++V GL+ + +GD+I+ T EH + Q +A +
Sbjct: 70 ALAETFNVDPNTITITDNVTTGCDIVLWGLD--WHQGDEILLTDCEHPGIIAIVQAIAAR 127
Query: 128 KGLKLEFIEGD---DEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEVEELGKMVKEEG 184
G+ F ++G+ A +LV + H+ G + + E+ + +
Sbjct: 128 FGITYRFFPVAATLNQGDAAAVLANHLGPKTRLVILSHLLWNTGQVLPLAEIMAVCRRHQ 187
Query: 185 AIF----VVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPP 240
+ +VD AQS G + +D +L+ D+ AF+GHK GP G+G LYI+ + P
Sbjct: 188 GNYPVRVLVDGAQSAGSLPLDFSRLEVDYYAFTGHKWFAGPAGVGGLYIHGDCLGEINPT 247
Query: 241 LIGGGTIE-DVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKL 299
+G +I + E +RFE T GL A ++ ++ G E++ + +
Sbjct: 248 YVGWRSITYGAKGEPTGWAEGGKRFEVATSAYPQYAGLLAALQLHQRQG--TAEERYQAI 305
Query: 300 VKRTTEGLDELEIPWYGPRNLD---------KHAGVVSFNV-PPLHPHDVASVLDEHKIM 349
+R+ E W G L AG+VSF V PL + L+E +I
Sbjct: 306 CQRS-------EFLWRGLNQLPHVHCLATSAPQAGLVSFTVDSPLGHRAIVQKLEEQRIY 358
Query: 350 VRSGHHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEE 392
+R+ + +RA H EE+ L L +
Sbjct: 359 LRT-----------IADPDCIRACCHYITDEEEINHLLARLAD 390
>sp|P31672|NIFS_LACDE NIFS PROTEIN HOMOLOG >gi|98662|pir||S16047 nitrogenase cofactor
synthesis protein nifS - Lactobacillus delbrueckii
>gi|43985|emb|CAA43493.1| (X61190) nifS-like gene
[Lactobacillus delbrueckii]
Length = 355
Score = 87.0 bits (212), Expect = 3e-16
Identities = 96/356 (26%), Positives = 155/356 (42%), Gaps = 43/356 (12%)
Query: 51 VHRLSQMATQKYEESRKVVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKK-GDKIVT 109
+H+L A E SRK VAD + DEI FT +ES N G ++ G I+T
Sbjct: 23 LHKLGDRAHGLLEASRKQVADLLGVNTDEIYFTSGGTESNNTAIKGTAWAKREFGKHIIT 82
Query: 110 TPYEHHSNLLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALG 168
+ EH S + L + G ++ + D EG ++ D + + K LV++ V+N +G
Sbjct: 83 SSVEHASVANTFTEL-ENLGFRVTRLPVDKEGRVNPEDLKAALDKDTTLVSIMGVNNEIG 141
Query: 169 VIHEVEELGKMVKEEGAI-FVVDAAQSVGHMEVD-VKKLKADFLAFSGHKGPMGPTGIGV 226
I ++E+ +++ + I F VD Q++G D V + D ++FS HK GP GIG+
Sbjct: 142 TIQPIKEISEILADYPNIHFHVDNVQALGKGIWDQVFTSRVDMMSFSSHKF-HGPRGIGI 200
Query: 227 LYINKEFFDVFEPPLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEK 286
LY K+ + P GGG + +GT N+ +AA K
Sbjct: 201 LY--KKRGRMLMPLCEGGG--------------QEKGLRSGTENLA---AIAAMAKAARL 241
Query: 287 IGIDKIEKQERKL-----VKRTTEGLDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVAS 341
+ D+ EK +R+ + + G + I + P D ++ F + + +
Sbjct: 242 LLTDEKEKADREYAIKEKISKYLAGKPGIHI--FSPLKADFAPHILCFALEGIRGETLVH 299
Query: 342 VLDEHKIMVRSGHHCA---------LPVMKRLKINGT--VRASFHVYNSLEEVETF 386
L++ I + + CA L MK T VR SF N+LEE + F
Sbjct: 300 TLEDQDIYISTTSACASKKADEASTLVAMKTPDAIATSAVRLSFDESNTLEEADEF 355
>pir||S77167 L-cysteine desulfhydrase (EC 4.4.1.-) - Synechocystis sp. (strain
PCC 6803) >gi|1652806|dbj|BAA17725.1| (D90908)
isopenicillin N epimerase [Synechocystis sp.]
Length = 401
Score = 87.0 bits (212), Expect = 3e-16
Identities = 100/415 (24%), Positives = 171/415 (41%), Gaps = 57/415 (13%)
Query: 8 RKDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRK 67
R+ P YF+ + P +EA+ Y + N + +Q Q + R+
Sbjct: 11 RQQFPGLANKTYFNFGGQGILPTVALEAITAMYGYLQENGPFSI-AANQYIQQLIAQLRQ 69
Query: 68 VVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKK 127
+ + N + + I T N + ++V G++ ++KGD+I+ T EH + Q +A +
Sbjct: 70 ALGETFNVDPNTITITDNVTTGCDIVLWGID--WQKGDEILLTDCEHPGIIAIVQAIAAR 127
Query: 128 KGLKLEFIEGDDEGNLDLADAEKKIKG-----AKLVAVQHVSNALGVIHEVEELGKMVKE 182
G+ F D L+ DA + +LV + H+ G + + E+ + +
Sbjct: 128 FGVTYRFFPVAD--TLNQGDAAAVLVNHLGPKTRLVILSHLLWNTGQVLPLGEIMAVCRR 185
Query: 183 EGAIF----VVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYI--------N 230
+ +VD AQS G + +D +L+ D+ AF+GHK GP G+G LYI N
Sbjct: 186 HQGDYPVRVLVDGAQSAGSLPLDFSRLEVDYYAFTGHKWFAGPAGVGGLYIHGDAKGTAN 245
Query: 231 KEFFDVFEPPLIGGGTI---EDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKI 287
P +G +I E + E +RFE T GL A ++ ++
Sbjct: 246 AARLGEINPTYVGWRSITYGSKGEPTGW--AEGGKRFEVATSAYPQYAGLLAALQLHQRQ 303
Query: 288 GIDKIEKQERKLVKRTTEGLDELEIPWYGPRNL---------DKHAGVVSFNV-PPLHPH 337
G E++ + + +R+ E+ W G L AG+VSF + PL
Sbjct: 304 G--TAEERYQAICQRS-------ELLWQGLNQLPHVHCLATSPPQAGLVSFTIDSPLGHR 354
Query: 338 DVASVLDEHKIMVRSGHHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEE 392
+ L+E +I +R+ + VRA H + EE+E L L E
Sbjct: 355 AIVQKLEEQRIYLRT-----------IADPDCVRACCHYITNEEEIEHLLSRLAE 398
>pdb|1ELU|A Chain A, Complex Between The Cystine C-S Lyase C-Des And Its
Reaction Product Cysteine Persulfide.
>gi|7767012|pdb|1ELU|B Chain B, Complex Between The
Cystine C-S Lyase C-Des And Its Reaction Product
Cysteine Persulfide. >gi|7767009|pdb|1ELQ|A Chain A,
Crystal Structure Of The Cystine C-S Lyase C-Des
>gi|7767010|pdb|1ELQ|B Chain B, Crystal Structure Of The
Cystine C-S Lyase C-Des
Length = 390
Score = 86.6 bits (211), Expect = 5e-16
Identities = 93/392 (23%), Positives = 164/392 (41%), Gaps = 41/392 (10%)
Query: 19 YFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEFD 78
YF+ + P +EA+ Y + N + +Q Q + R+ +A+ N + +
Sbjct: 19 YFNFGGQGILPTVALEAITAMYGYLQENGPFSI-AANQHIQQLIAQLRQALAETFNVDPN 77
Query: 79 EIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEGD 138
I T N + ++V GL+ + +GD+I+ T EH + Q +A + G+ F
Sbjct: 78 TITITDNVTTGCDIVLWGLD--WHQGDEILLTDCEHPGIIAIVQAIAARFGITYRFFPVA 135
Query: 139 ---DEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIF----VVDA 191
++G+ A +LV + H+ G + + E+ + + + +VD
Sbjct: 136 ATLNQGDAAAVLANHLGPKTRLVILSHLLWNTGQVLPLAEIMAVCRRHQGNYPVRVLVDG 195
Query: 192 AQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIE-DV 250
AQS G + +D +L+ D+ AF+GHK GP G+G LYI+ + P +G +I
Sbjct: 196 AQSAGSLPLDFSRLEVDYYAFTGHKWFAGPAGVGGLYIHGDCLGEINPTYVGWRSITYGA 255
Query: 251 ELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDEL 310
+ E +RFE T GL A ++ ++ G E++ + + +R+
Sbjct: 256 KGEPTGWAEGGKRFEVATSAYPQYAGLLAALQLHQRQG--TAEERYQAICQRS------- 306
Query: 311 EIPWYGPRNLD---------KHAGVVSFNV-PPLHPHDVASVLDEHKIMVRSGHHCALPV 360
E W G L AG+VSF V PL + L+E +I +R+
Sbjct: 307 EFLWRGLNQLPHVHCLATSAPQAGLVSFTVDSPLGHRAIVQKLEEQRIYLRT-------- 358
Query: 361 MKRLKINGTVRASFHVYNSLEEVETFLGVLEE 392
+ +RA H EE+ L L +
Sbjct: 359 ---IADPDCIRACCHYITDEEEINHLLARLAD 387
>pir||F70695 hypothetical protein Rv3778c - Mycobacterium tuberculosis (strain
H37RV) >gi|1552861|emb|CAB02457.1| (Z80343) hypothetical
protein Rv3778c [Mycobacterium tuberculosis]
Length = 398
Score = 85.0 bits (207), Expect = 1e-15
Identities = 69/272 (25%), Positives = 119/272 (43%), Gaps = 22/272 (8%)
Query: 18 IYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEF 77
++FD A L P V + + R A+ G H ++ + + +R+ VAD +NA+
Sbjct: 20 VHFDAPAGMLIPDSVATTVSTAFRRSGASTV-GAHPSARRSAAVLDAAREAVADLVNADP 78
Query: 78 DEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEG 137
+ + + L+L+A G +++ + + +N+ PW R A + G K+++ E
Sbjct: 79 GGVVLGADRAVLLSLLAEASSSRAGLGYEVIVSRLDDEANIAPWLRAAHRYGAKVKWAEV 138
Query: 138 DDE-GNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSV 195
D E G L E I K +LVAV S LG + ++ + K+V + GA+ VVD + +
Sbjct: 139 DIETGELPTWQWESLISKSTRLVAVNSASGTLGGVTDLRAMTKLVHDVGALVVVDHSAAA 198
Query: 196 GHMEVDVKKLKADFLAFSGHKGPMGPTGIGVL---YINKEFFDVFEPPLIGGGTIEDVEL 252
+ +D+++ AD + + H P G V + F V P G
Sbjct: 199 PYRLLDIRETDADVVTVNAHAWGGPPIGAMVFRDPSVMNSFGSVSTNPYATG-------- 250
Query: 253 CCYKLTEPPERFEAGTPNIGGAIGLAAGIKYI 284
P R E G G G+ A I+Y+
Sbjct: 251 --------PARLEIGVHQFGLLAGVVASIEYL 274
>sp|P70727|NIFS_AZOBR NIFS PROTEIN >gi|1597739|gb|AAC46177.1| (U26427) nifS [Azospirillum
brasilense]
Length = 398
Score = 85.0 bits (207), Expect = 1e-15
Identities = 72/236 (30%), Positives = 105/236 (43%), Gaps = 10/236 (4%)
Query: 13 LTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADF 72
+T + IY DN AT+ V+ M + N +H + + A
Sbjct: 1 MTAQGIYLDNNATTRVDPDVLAEMLPLFTEQFGNPS-SMHGFGAAVGGQDRMGAQAGAGA 59
Query: 73 INAEFD-EIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLK 131
A D EI FT +ES N L + K IVT+ EH + L L KK+G
Sbjct: 60 ARAAHDSEIVFTSGGTESDNTAILSTLEAYPKKKSIVTSVVEHPAVLALCDYLEKKRGYT 119
Query: 132 LEFIEGDDEGNLDLADAEKKI---KGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFV 188
+ I D+ GNLDL DA K+ +V++ +N G I +EEL ++ K GA+F
Sbjct: 120 VHRIGVDNRGNLDL-DAYKRCALGPDVAIVSIMWANNETGTIFPIEELAQLAKAAGAVFH 178
Query: 189 VDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGG 244
A Q+VG + ++V+ D L GHK P G+ LY+ + F P + GG
Sbjct: 179 T-AVQAVGKIPMNVRHSAVDMLCACGHK-LHAPKGVHALYVKRGL--RFRPIVRGG 230
>gb|AAF36034.1| (AC024202) contains similarity to Escherichia ecoli hypothetical
protein (SP:P76055) [Caenorhabditis elegans]
Length = 1512
Score = 84.3 bits (205), Expect = 2e-15
Identities = 70/248 (28%), Positives = 114/248 (45%), Gaps = 22/248 (8%)
Query: 15 QEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFIN 74
+ + Y D TA++ + + L + N H V ++ T E+R+ + F
Sbjct: 548 ENMTYCDYTASARAFTSIETYIQHEVLPFYGNTHSSVTVTAEQTTLFMHEARQEIRAFSG 607
Query: 75 A-EFDEIAFTKNTSESLNLVALGLEHLFKKGDK-----IVTTPYEHHSNLLPWQRLAKKK 128
+ D + FT S S V L L HL G +V + EHHSNLLPW+++AK+
Sbjct: 608 CGDQDSVIFTG--SGSTCAVEL-LVHLMSDGKDDEEVIVVHSSQEHHSNLLPWRKIAKE- 663
Query: 129 GLKLEFIEGDDEGNLDLADAEKKIK-------GAKLVAVQ-HVSNALGVIHEVEELGKMV 180
IE + G +DL + +K K++ SN G++ +++ + K++
Sbjct: 664 ---CRNIEELENGQIDLVHLQNVLKEIRESHGSIKIIGTFCAASNLTGILIDIQNVTKIL 720
Query: 181 KEEGAIFVVDAAQSVGHMEVDVK-KLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEP 239
K AI + D A + ++ + V D + FSGHK P G + GVL + K +P
Sbjct: 721 KMFDAIAIWDFASAAPYIPILVNGTYPLDAVFFSGHKFPGGVSSPGVLIVKKSIIQATKP 780
Query: 240 PLIGGGTI 247
IGGGT+
Sbjct: 781 KRIGGGTV 788
>pir||H72558 hypothetical protein APE1754 - Aeropyrum pernix (strain K1)
>gi|5105444|dbj|BAA80757.1| (AP000062) 415aa long
hypothetical protein [Aeropyrum pernix]
Length = 415
Score = 83.9 bits (204), Expect = 3e-15
Identities = 90/405 (22%), Positives = 157/405 (38%), Gaps = 42/405 (10%)
Query: 6 DVRKDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEES 65
+VR+ IP+ +Y D V +A++ + + + L + ES
Sbjct: 32 EVRERIPIFSRKVYLDTAGAGPLTLDVKDAVEGFLELWNSEGEPWGEALDDIL-----ES 86
Query: 66 RKVVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLA 125
R++ A + A+ +E+A + LN L+ +V + + + + +A
Sbjct: 87 RRLFAQMVGAKVEEVAAVPGVTYGLNAFLSSLK--LPPDSNVVVSETNFPTGVYTFHAMA 144
Query: 126 KKKGLKLEFIEGDDEGNLDLADAEKKIKGA-KLVAVQHVSNALGVIHEVEELGKMVKEEG 184
++ ++ I +G + L E+ I + +V V VS G ++ L ++ G
Sbjct: 145 RRGLIREVRIASASDGCVGLDGFERLIDDSTSIVYVDMVSWITGCREDLRTLAELAHSHG 204
Query: 185 AIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGG 244
A+ V D + G +DVKKL D +K +G G G Y+++E EP G
Sbjct: 205 ALMVSDVFHAAGVYPIDVKKLGLDAAFTGSYKWLLGLHGGGFAYVSEELLPSLEPMFSGW 264
Query: 245 GTIED----------------VELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIG 288
I+D + ++ + E GT + L A +K +
Sbjct: 265 MAIDDSVMERMARGDPLFSRPFNVREFRRASDAKVLEWGTWPAIAFVSLKASLKMLSAYD 324
Query: 289 IDKI-EKQERKLVKRTTEGLDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHK 347
+ +LVKR + L ++ + PR + AG+V F HP + L+
Sbjct: 325 APGLYASHTGRLVKRLIDSLTDMGVETVTPRGM--AAGIVVFR--HRHPERLGRFLESRG 380
Query: 348 IMVRSGHHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEE 392
I+ G+VR S H YNS E+VE L LEE
Sbjct: 381 IVASVR-------------PGSVRVSIHFYNSREDVEALLRALEE 412
>gb|AAD17272.1| (AF119361) NifS [Frankia sp. EuIK1]
Length = 419
Score = 82.7 bits (201), Expect = 7e-15
Identities = 80/313 (25%), Positives = 122/313 (38%), Gaps = 32/313 (10%)
Query: 17 VIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAE 76
+IY D+ AT+ V+E M + AN H Q AT+ E +R+ VA A
Sbjct: 1 MIYLDHNATTPVDPRVVEVMLPLFTERFANP-ASKHAPGQEATRIVERARREVASLAGAR 59
Query: 77 FDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKL---- 132
EI FT +E+ NL G+ + G + + H +L A G +
Sbjct: 60 PREIVFTSGATEAANLAIGGVLATAEPGRRRILVGATEHPAVLGAADAAVSAGSAVSADG 119
Query: 133 ----EFIEGDDEGNLDLADAEKKI-KGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIF 187
E I +G +D+ D +++ LVAV +N G +++ L + + G
Sbjct: 120 TTAAERIAVHPDGTVDVDDLRRRLGPDVALVAVMAANNETGAVNDPRPLVEAARSAGRCC 179
Query: 188 VVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTI 247
D Q++G + V+ ++ D S HK GP G+G L +K+ + P + GGG
Sbjct: 180 CADVTQALGRVPVEFERTGVDLAVSSAHK-LYGPKGVGALIASKDAWSRLAPRVHGGG-- 236
Query: 248 EDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQE-------RKLV 300
AGT N G G A + + D +Q LV
Sbjct: 237 ------------QERGLRAGTLNTAGIAGFGAAARIAAESMADDGTRQRALVALLTELLV 284
Query: 301 KRTTEGLDELEIP 313
KR G EL P
Sbjct: 285 KRLGPGAVELNGP 297
>sp|P38033|NIFS_BACSU NIFS PROTEIN HOMOLOG >gi|421514|pir||B47071 nitrogenase cofactor
synthesis protein nifS - Bacillus subtilis
>gi|143241|gb|AAA21613.1| (M98822) homologous to the
nifS gene products of other bacteria [Bacillus subtilis]
>gi|2635253|emb|CAB14748.1| (Z99118) nifS [Bacillus
subtilis]
Length = 395
Score = 80.0 bits (194), Expect = 4e-14
Identities = 81/302 (26%), Positives = 131/302 (42%), Gaps = 33/302 (10%)
Query: 17 VIYFDNTATSLTPKPVIEAMDEYYLRYRANVH---RGVHRLSQMATQKYEESRKVVADFI 73
+IY D AT+ P+ E Y + +++ +H A E R+ +A+ I
Sbjct: 1 MIYLDYAATT----PICEEALTVYQKLSMDMYGNASSLHDAGGKAKHILEYCREKIANII 56
Query: 74 NAEFDEIAFTKNTSESLNLVALG--LEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLK 131
E I FT +ES N +A+ L L K +TT EH S + +++G
Sbjct: 57 GGEASGIYFTSGGTES-NFLAIQSLLNGLPKTKRHFITTAMEHQS-IHNCAAFLEQQGYD 114
Query: 132 LEFIEGDDEGNLDLADAEKKIKGAK-LVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVD 190
+ IE ++ G + I+ LV++QH ++ G+I ++ L + +G + D
Sbjct: 115 VTVIEPNEYGLITEEILLTHIRPETGLVSIQHANSETGIIQPIQHLSSYLHNKGILLHCD 174
Query: 191 AAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDV 250
A Q+ G + ++ K L D L+ S HK GP G+G +YI DV P+
Sbjct: 175 AVQTFGKIPINTKNLGVDALSMSSHK-IHGPKGVGAVYIRP---DVPWKPV--------- 221
Query: 251 ELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDEL 310
Y LT F AGT N+ G A + I + ++EKQ + T LD++
Sbjct: 222 ----YPLTTHEYGFRAGTVNVPGIGAFTAAAELI----VSEMEKQISRNEALRTYFLDQI 273
Query: 311 EI 312
I
Sbjct: 274 RI 275
>gb|AAF12840.1|AF203881_13 (AF203881) NifS [Zymomonas mobilis]
Length = 370
Score = 80.0 bits (194), Expect = 4e-14
Identities = 103/397 (25%), Positives = 163/397 (40%), Gaps = 46/397 (11%)
Query: 17 VIYFDNTATSLTPK-PVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFI-N 74
+IY D AT TP P +A +L AN H HR+ + A ++ +A I
Sbjct: 1 MIYLDYQAT--TPMAPEAKAAMLPWLDGGANPH-SPHRIGRKAAAAVAIAKDQIAALIPE 57
Query: 75 AEFDEIAFTKNTSESLNLVALGLEHLFKKGD-KIVTTPYEHHSNLLPWQRLAKKKGLKLE 133
E + FT +E+LN+ GL K KIVT EH + L LA+ G ++
Sbjct: 58 REAGNLVFTSGATEALNMAIKGLWFSGKNTRRKIVTLATEHAATLDSLGYLARL-GAEIV 116
Query: 134 FIEGDDEGNLDLADAEKKIKG-AKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAA 192
+ +G +DL A I LV V+N +GVI + EL K+ + GA F++DA
Sbjct: 117 LLPVHSDGLVDLEKAAAAIDDKTALVTAMFVNNEIGVIQPIAELAKLACQVGAYFLLDAV 176
Query: 193 QSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVEL 252
Q G + D +A SGHK P G G L+ K D+ PL+ GG
Sbjct: 177 QGYGRFALPE---GCDMIALSGHK-IHAPIGSGALWFKK---DITLTPLLHGG------- 222
Query: 253 CCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELEI 312
E F +GT + L + + G ++ K + + L + E+
Sbjct: 223 ------HQQEGFRSGTLS-----PLCLRLWCCRRFGAEQQAKDQSHVA--ALFALAKAEM 269
Query: 313 PWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCA------LPVMKRL-- 364
P + + + +FN+ + D ++ +G C+ V+K L
Sbjct: 270 PDWIINGSEDQRWLGNFNIHQEGIDSSRLISDARQVAFSAGSACSSGNGRPSHVLKALGL 329
Query: 365 ---KINGTVRASFHVYNSLEEVETFLGVLEELVKSLR 398
+ G++R F Y + EE+ + LG + E + R
Sbjct: 330 SQKAVRGSIRIGFGRYTTEEELLSGLGYIREAAEKQR 366
>sp|Q59072|YG78_METJA HYPOTHETICAL PROTEIN MJ1678 >gi|2129014|pir||D64509 hypothetical
protein MJ1678 - Methanococcus jannaschii
>gi|1500582|gb|AAB99700.1| (U67608) acetyltransferase
[Methanococcus jannaschii]
Length = 396
Score = 77.6 bits (188), Expect = 2e-13
Identities = 96/393 (24%), Positives = 176/393 (44%), Gaps = 62/393 (15%)
Query: 27 LTPKPVIEAMDEYYLRYRANVHRGVH-RLSQMATQKYEESRKVVADFINAEFDEIAFTKN 85
+ PK +A+ EY+ Y +V H RL ++ ++ + +A F+N D T
Sbjct: 45 ILPKEAKKAVYEYWDGY--SVCDYCHGRLDEVTCPPIKDFLEDIAKFLN--MDCARPTHG 100
Query: 86 TSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRL---AKKKGLKLEF--IEGDDE 140
E +V + + K+GD +V H+++ + +R + G + E+ + + E
Sbjct: 101 AREGKFIV---MHAICKEGDYVVLDKNAHYTSYVAAERAKLNVAEVGYEEEYPTYKINLE 157
Query: 141 GNLDLAD-AEKKIKGAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVGHME 199
G ++ D E K K L+ + HV G +++ +++GK+ KE+G F+++ A +VG M
Sbjct: 158 GYKEVIDNLEDKGKNVGLILLTHVDGEYGNLNDAKKVGKIAKEKGIPFLLNCAYTVGRMP 217
Query: 200 VDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCYKLTE 259
V+ K++KADF+ SGHK G+L ++EF D K+T+
Sbjct: 218 VNGKEVKADFIVASGHKSMAASAPCGILAFSEEFSD--------------------KITK 257
Query: 260 PPERFEAGTPNIGGA-------IGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGLDELEI 312
E+F + G + L A ++ +++++K + +L K+T +DELE
Sbjct: 258 TSEKFPVKEIEMLGCTSRGLPIVTLMASFPHV----VERVKKWDEEL-KKTRYVVDELEK 312
Query: 313 PWYGPRNL-DKHAGVVSFNVPPLHPHDVASVLDE-HKIMVRSGHHCALPVMKR----LKI 366
+ + K ++ F P VLDE K R G + KR ++
Sbjct: 313 IGFKQLGIKPKEHDLIKFETP---------VLDEIAKKDKRRGFFFYDELKKRGIGGIRA 363
Query: 367 NGTVRASFHVYN-SLEEVETFLGVLEELVKSLR 398
T VY E+VE + ++E+V+S +
Sbjct: 364 GVTKEIKMSVYGLEWEQVEYVVNAIKEIVESCK 396
>pir||T23860 hypothetical protein R03A10.3 - Caenorhabditis elegans
>gi|3878874|emb|CAA93672.1| (Z69793) R03A10.3
[Caenorhabditis elegans]
Length = 548
Score = 76.5 bits (185), Expect = 5e-13
Identities = 81/343 (23%), Positives = 144/343 (41%), Gaps = 38/343 (11%)
Query: 19 YFDNTATSLTPKPVIEAMDEYYLRY-RANVHRGVHRLSQMATQKYEESRKV-VADFINAE 76
Y D+ ++L K +E + + + AN H H + + T++ S ++ + + N
Sbjct: 3 YLDHAGSTLPSKIQLEEVAKQQSQLILANPHS--HHATAVKTKQIVNSARLRILQYFNTT 60
Query: 77 FDE--IAFTKNTSESLNLVA------------LGLEHLFKKGDKIVTTPYEHHSNLLPWQ 122
D+ + T NT+ L +VA L + + G + Y+ H +++ +
Sbjct: 61 SDDYFVVLTNNTTHGLKIVAENFKFGQKTHSILNIASVLHGGSSNLGYLYDSHHSVVGLR 120
Query: 123 RLAKKKGLKLEFIEGDDEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEVEELGKMVKE 182
+ K + + + ++ D E L + +SN G + +E + ++ +E
Sbjct: 121 HVVNGKVNSISCVNEESILEHEIPDVEHS-----LFVLTAMSNFCGKKYSLESVHRL-QE 174
Query: 183 EGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLI 242
+G +DAA V +D+ + + +F+AFS +K PTGIG L + K+ + E
Sbjct: 175 KGWAVCLDAASFVSSSALDLSQQRPNFIAFSFYKIFGYPTGIGALLVRKDSAHLIEKTSF 234
Query: 243 GGGTIEDVE--LCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKI-GIDKIEKQERKL 299
GGT++ V+ + L E FE GT N G L G + IE+ GI I L
Sbjct: 235 AGGTVQSVDEMSMFFVLREFERAFEEGTLNYYGIAQLQKGFEEIERCGGISSIRNLTHHL 294
Query: 300 VKRTTEGLDELEIPWYGP-----------RNLDKHAGVVSFNV 331
K L + P P N DK +V+FN+
Sbjct: 295 CKNALYMLKSKKHPNGRPVVEIYSQSEQFENPDKQGPIVAFNL 337
>emb|CAA21971.1| (AL033497) unknown hypothetical protein [Candida albicans]
Length = 421
Score = 75.7 bits (183), Expect = 9e-13
Identities = 103/416 (24%), Positives = 171/416 (40%), Gaps = 47/416 (11%)
Query: 13 LTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADF 72
+ EV ++ + LTP PV+E + ++ + + + Y ES K V
Sbjct: 20 MDDEVFPVNHGSYGLTPTPVLEKYLDLIVKNASYTDKF---MKYGIKDSYVESLKAVGKV 76
Query: 73 INAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIV--TTPYEHHSNLLPWQRLAKKKGL 130
+N ++ +AF N + +N + + KK DK+V +T Y N + + L + G+
Sbjct: 77 LNCDYHNLAFVDNATSGVNTILRS--YPLKKVDKLVIQSTVYGACGNTVKF--LHDRYGV 132
Query: 131 KLEFIEGD------DEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEVEELGKMVKEEG 184
EFI D DE L + + KL +S+ GVI E++ K+ K+
Sbjct: 133 --EFIVVDLNYPITDEEILSKFERVFVEEKPKLCMFDTISSMPGVIFPYEKMTKLCKKYS 190
Query: 185 AIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGG 244
+ ++D A +G + D+ LK DF + HK P G VLYI+ + +V I
Sbjct: 191 VLSLIDGAHGIGCIPQDLGNLKPDFYVTNLHKWFYIPFGCAVLYIDPKHHNVVHTLPISH 250
Query: 245 GTIEDVELCCYKLTEPPER-------FEAGTPNIGGAIGLAAGIKYIEKI--GIDKIEKQ 295
+ED KL++ ++ F GT N + IK+ ++ G KI
Sbjct: 251 SYLED----HVKLSDGDQKNRLIDRFFLYGTKNFASIQVIPEAIKFRSEVCGGETKIYDY 306
Query: 296 ERKLVKRTTEGLDELEIPWYGPRNLDKHAG-----VVSFNVPPLHPHDVA---SVLDEHK 347
L K+ + EL +G LD+ +V+ VP ++ SV+D H
Sbjct: 307 CHGLAKQ----VGELVSRKWGTSYLDQKGSTLISTMVTVEVPTQDYPEIVKNWSVIDHHV 362
Query: 348 IMVRSGHHCALPVMKRLKINGTVRASF--HVYNSLEEVETFLGVLEELVKSLRSSQ 401
P + NG + A F VYN L + + V+ E +K + S Q
Sbjct: 363 YNKMFDRKAYTPCIAH---NGKLFARFSCQVYNELSDYDYASDVVLETLKEVASEQ 415
>pir||E75569 probable aminotransferase - Deinococcus radiodurans (strain R1)
>gi|6457687|gb|AAF09620.1|AE001866_7 (AE001866)
aminotransferase, putative [Deinococcus radiodurans]
Length = 544
Score = 74.9 bits (181), Expect = 1e-12
Identities = 76/267 (28%), Positives = 116/267 (42%), Gaps = 24/267 (8%)
Query: 111 PYEHHSNLLPWQR-LAKKKGLKLEFIEGDDEGNLDLADAEKKIKGA------KLVAVQHV 163
PYEHHSN + W+ LA+ + L GNLDL +K K+ +
Sbjct: 148 PYEHHSNEVSWRETLAEVVEIPL-----CARGNLDLDALITALKDPRYARRPKIGSFSAA 202
Query: 164 SNALGVIHEVEELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKAD---FLAFSGHKGPMG 220
SN G++ + + +++ GA D A S ++ +D+K + D + S HK G
Sbjct: 203 SNVTGLLTDTRTVARILHRHGAYAFFDFAASAPYVAIDMKPGQPDGYDAVFLSPHKFVGG 262
Query: 221 PTGIGVLYINKEFFDVFEPPLIGGGTIEDVELCCYKLTEPPE-RFEAGTPNIGGAIGLAA 279
P G+L E + + P GGGT+ V + + E R +AGTP I G I A
Sbjct: 263 PGTPGLLCFRAELYRLSSPSTAGGGTVRYVSRTKHTFVDDIEAREDAGTPAILGKIRTAL 322
Query: 280 GIKYIEKIGIDKIEKQERKLVKRTTEGLD-ELEIPWYGPRNLDKHAGVVSFNVPP----- 333
+ E++G+ + ++E +L R L + G + + A +SF V
Sbjct: 323 AFRVKEELGVRALTEREHELFGRAVTRLGIHPRVQLLGNPDAPRLA-FLSFLVKAEDGTY 381
Query: 334 LHPHDVASVL-DEHKIMVRSGHHCALP 359
LHP V +L D I R G CA P
Sbjct: 382 LHPRFVVRLLNDLFGIQARGGCACAGP 408
>pir||E83544 hypothetical protein PA0813 [imported] - Pseudomonas aeruginosa
(strain PAO1) >gi|9946706|gb|AAG04202.1|AE004516_7
(AE004516) hypothetical protein [Pseudomonas aeruginosa]
Length = 415
Score = 74.9 bits (181), Expect = 1e-12
Identities = 91/409 (22%), Positives = 165/409 (40%), Gaps = 28/409 (6%)
Query: 7 VRKDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESR 66
+R++ P +F + + SL P+ + EA+D + A GV R ++ + R
Sbjct: 20 LRRETPAWGSYAHFAHGSASLPPQVLYEALDSWL---EAERRWGVQRAAEHFAEPLLAVR 76
Query: 67 KVVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAK 126
VA + + IA S + + VA + +++ E+ SN L +
Sbjct: 77 DSVARVLGTQPRHIALLDCASRAWS-VAFAAALAAHPRIRAISSFDEYGSNSLCLLAARQ 135
Query: 127 KKGLKLEFIE--GD--------DEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEVEEL 176
++GL+L I+ GD DE DLA + + V H G +E +
Sbjct: 136 QRGLELRLIDARGDAAQLLQRLDEQLHDLAPGQTPLLSLSAVPTGH-----GAATALEGV 190
Query: 177 GKMVKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDV 236
+ ++ +F +DA+ +VG + + V+ D L F K GP G+GVLY+ + +
Sbjct: 191 AERIRAHDGLFFLDASHAVGQLPLAVEATGCDVLVFPPRKWLRGPKGLGVLYLGERALER 250
Query: 237 FEPP---LIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIE 293
P +GG D + + RFE N G + L A Y+ + + +I
Sbjct: 251 LALPDGLDVGGAQWSDA--FALQARDDARRFECSEFNPGLRLALKASCDYLLQTDVRRIA 308
Query: 294 KQERKLVKRTTEGLDELEIPWYGPRNLDKHA-GVVSFNVPPLHPHDVASVLDEHKIMVR- 351
++ R+L +R + L + W P HA ++++ P L L +
Sbjct: 309 RRNRQLRERIAQQLYR-RLGW-TPLEQGPHASALMTYAAPELSGEQWLKRLHARGVNASY 366
Query: 352 SGHHCALPVMKRLKINGTVRASFHVYNSLEEVETFLGVLEELVKSLRSS 400
G A ++ + G +R + H E+E LE+ ++ +S
Sbjct: 367 IGPQYARWALREQALPGVLRLTPHYLTDDGEIERLGEALEDCLRQRAAS 415
>sp|P18549|CEFD_STRCL ISOPENICILLIN N EPIMERASE >gi|153204|gb|AAA26714.1| (M32324)
isopenicillin N epimerase (cefD) [Streptomyces
clavuligerus]
Length = 398
Score = 74.5 bits (180), Expect = 2e-12
Identities = 57/226 (25%), Positives = 99/226 (43%), Gaps = 20/226 (8%)
Query: 64 ESRKVVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQR 123
++R+ +A I + +A N + ++NLVA L + +I+ + E+ W+R
Sbjct: 63 QARESLARLIGGDPLRLALATNVTAAVNLVASSLR--LEAPGEILLSDDEYTPMRWCWER 120
Query: 124 LAKKKGLKLEFIE----GDDEGNLDLADAEKKIKGAKLVAVQHVSNALGVIHEVEELGKM 179
+A++ GL+L D + A +L HV + G+I EL +
Sbjct: 121 VARRHGLELRTFRLPELPSDPAEITAAAVAAMGPRTRLFFFSHVVSTTGLILPAAELCEE 180
Query: 180 VKEEGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEP 239
+ G VVD A + G +++D+ ++ DF A SGHK + PTG+G L++ + EP
Sbjct: 181 ARARGITTVVDGAHAPGFLDLDLSRIPCDFYAGSGHKWLLAPTGVGFLHLAPGRLEELEP 240
Query: 240 PLIGGGTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIE 285
+ EPPE +G P G G++ +E
Sbjct: 241 TQVSWA------------YEPPE--GSGPPAARDRFGSTPGLRRLE 272
>pir||B75547 iron-sulfur cofactor synthesis protein DR0215 [similarity] -
Deinococcus radiodurans (strain R1)
>gi|6457882|gb|AAF09798.1|AE001883_4 (AE001883)
aminotransferase, class V [Deinococcus radiodurans]
Length = 378
Score = 74.5 bits (180), Expect = 2e-12
Identities = 63/235 (26%), Positives = 101/235 (42%), Gaps = 13/235 (5%)
Query: 17 VIYFDNTATS-LTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINA 75
+IY D AT LTP + + L VH Q A +K EE R +A +
Sbjct: 2 MIYLDYAATHPLTPAALAAYAEAAALPGNP---ASVHAAGQAAREKLEEGRARMAAAFDV 58
Query: 76 EFDEIAFTKNTSESLNLVALGLEHLFKKGD----KIVTTPYEHHSNLLPWQRLAKKKGLK 131
+ + +E N V LG +++ +VTTP EH + L P + LA G
Sbjct: 59 DPRTLTLNSGGTEGDNHVLLGAAQAWQERHGSPGHLVTTPTEHSAVLAPARHLA-AHGWD 117
Query: 132 LEFIEGDDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVD 190
+ ++E D G+ ++ LV++ H +N +G + L + G + D
Sbjct: 118 VTWLEPDSAGHYSPEQLGAALRDDTALVSIHHANNEIGTVQPTAALAAVAAARGVPYHTD 177
Query: 191 AAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGGG 245
A Q+ G + V++ + + FS HK GP G+G LY+ + P +GGG
Sbjct: 178 AVQAPGVLPVNLAEWGVTYATFSAHKWG-GPRGVGALYVQRG--AELPPVTLGGG 229
>pir||B71652 iron-sulfur cofactor synthesis protein RP487 [similarity] -
Rickettsia prowazekii >gi|3861040|emb|CAA14940.1|
(AJ235272) NIFS PROTEIN HOMOLOG (spl1) [Rickettsia
prowazekii]
Length = 376
Score = 73.4 bits (177), Expect = 4e-12
Identities = 96/400 (24%), Positives = 154/400 (38%), Gaps = 58/400 (14%)
Query: 17 VIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAE 76
+IY D+ AT+ V E + L + H + A E +R +A +
Sbjct: 1 MIYLDHNATTFIDPRVKEYIIS--LMDKELNPSSAHTSGRFAKNIIETARSQIATSLGIT 58
Query: 77 FDE---IAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEH-----HSNLLPWQRLAKKK 128
I FT + +ES NL+ F GD I + EH H N P
Sbjct: 59 MSREYNITFTSSGTESNNLIMKN----FYDGD-IFISAIEHLSIYNHINYAP-------- 105
Query: 129 GLKLEFIEGDDEGNLDLADAEKKIKGA----KLVAVQHVSNALGVIHEVEELGKMVKEEG 184
++ I + +G +DL E + + KLV+V +N GV+ ++ + K+ K+
Sbjct: 106 --NIKIIRVNKQGLVDLEHLEDLLSQSNASKKLVSVMIANNENGVLQDIAAISKITKKYN 163
Query: 185 AIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKEFFDVFEPPLIGG 244
A F D QS G + +++K+L DF SGHK G G G L N F + P +IGG
Sbjct: 164 AKFHSDLVQSFGKISLNIKELGLDFATISGHK-IGGGHGCGALISNSNFQLI--PIIIGG 220
Query: 245 GTIEDVELCCYKLTEPPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTT 304
G + V +GT N+ G ++ K I K + R L +R
Sbjct: 221 GQEKSV--------------RSGTENVLAIAGFGLAAEFRRK-DISKNYIKIRYLQERLE 265
Query: 305 EGLDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMKRL 364
+ L E N+ + A V D I V SG C+ + +
Sbjct: 266 QKLKEYSNVNIISNNVARLANTTLITVDNTDAQVKLIGFDLRNICVSSGSACSSGKISKS 325
Query: 365 KI-----------NGTVRASFHVYNSLEEVETFLGVLEEL 393
+ ++R S N++ +++ F+ EE+
Sbjct: 326 HVLTNMGIREEEARSSIRISLSHTNTISDIKAFIKAFEEI 365
>dbj|BAB04936.1| (AP001511) NAD biosynthesis [Bacillus halodurans]
Length = 375
Score = 71.4 bits (172), Expect = 2e-11
Identities = 57/220 (25%), Positives = 112/220 (50%), Gaps = 15/220 (6%)
Query: 17 VIYFDNTATSLTPKPVIEAMDEYYLRYRANVH---RGVHRLSQMATQKYEESRKVVADFI 73
+IY D AT+ P+ + + + Y+ + + +H + A+ E+ ++ +A+ +
Sbjct: 1 MIYLDYCATT----PMSDYVKDVYVTVASQFYGNPNSLHDVGYEASNILEQCKRQIANLL 56
Query: 74 NAEFDEIAFTKNTSES--LNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLK 131
+ + I FT + SE+ L +V+L L H +KG ++TT EH S L ++ L ++
Sbjct: 57 HIDPAGIFFTGSASEANFLTIVSLALAHR-RKGRHLITTKCEHPSVLSTFRYLESQQ-FD 114
Query: 132 LEFIEGDDEGNLDLADAEKKIK-GAKLVAVQHVSNALGVIHEVEELGKMVKEEGAIFVVD 190
+ ++ + G + + I+ LV++ H + LG I +E + + +F D
Sbjct: 115 VSYVPVNRYGQVSVDALMGTIREDTILVSIAHSQSVLGTIQSLETFAEPLVTYDILFHSD 174
Query: 191 AAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYIN 230
QS+G ++V +++L + L SGHK GP GIG+ Y++
Sbjct: 175 CTQSIGKVDVPIRQLHS--LTMSGHK-INGPKGIGLAYVD 211
>gb|AAF34417.1|AF172282_6 (AF172282) hypothetical protein, similar to H. pilory nifS like
protein [Oryza sativa]
Length = 659
Score = 70.2 bits (169), Expect = 4e-11
Identities = 58/193 (30%), Positives = 90/193 (46%), Gaps = 19/193 (9%)
Query: 111 PYEHHSNLLPWQRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKGAK------LVAVQHVS 164
PYEHHSNLL W+R +E GDD G LDLA + ++ + L + S
Sbjct: 220 PYEHHSNLLSWRRSLAD---VVEVGAGDD-GLLDLAALRRALRAPEHADRPMLGSFSACS 275
Query: 165 NALGVIHEVEELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKA---DFLAFSGHKGPMGP 221
N GV+ + + +++ + GA D A S ++E+D++ + D + S HK GP
Sbjct: 276 NVTGVLTDTRAVARLLHQHGAFACFDFAASGPYVEIDMRPGEVDGYDAVFLSPHKFVGGP 335
Query: 222 TGIGVLYINKEFFDVFE--PPLIGGGTIEDV----ELCCYKLTEPPERFEAGTPNIGGAI 275
G+L +++ + + P GGGT+ V E L ER +AGTP I G +
Sbjct: 336 GTPGILLMSRSLYRLSSQPPTTCGGGTVAYVNGASERDTVYLAGVEEREDAGTPPIVGKV 395
Query: 276 GLAAGIKYIEKIG 288
A ++G
Sbjct: 396 RAALAFWVKARVG 408
>pir||D83347 probable aminotransferase PA2394 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9948435|gb|AAG05782.1|AE004665_9 (AE004665) probable
aminotransferase [Pseudomonas aeruginosa]
Length = 427
Score = 70.2 bits (169), Expect = 4e-11
Identities = 56/240 (23%), Positives = 105/240 (43%), Gaps = 16/240 (6%)
Query: 7 VRKDIPLTQEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESR 66
+R+ L + ++F N + P+PV EA++ +R+ N V + + +E+R
Sbjct: 43 LRQQFDLDPQYLHFANFLLTSHPRPVREAIERLRVRFDRNPGEAVDWHREEIWKYEDEAR 102
Query: 67 KVVADFINAEFDEIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAK 126
+ + ++A T +T++ L + GL L + G +I+T+ +EH+S + K
Sbjct: 103 AWAGRYFAVQPGQVALTGSTTDGLAAIYGGL--LVQPGKEILTSSHEHYSTYTTLEYRHK 160
Query: 127 KKGLKL-EFIEGDDEGNLDLADAEKKIKG-----AKLVAVQHVSNALGVIHEVEELGKMV 180
+ G ++ EF D + + I +++ + V + GV + E+GK+V
Sbjct: 161 RMGTQVREFPLFKDPHRVSADEILSSIAAQIRPQTRVLGMTWVQSGSGVKLPIREIGKLV 220
Query: 181 KE--------EGAIFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVLYINKE 232
+E + I+VVD G +V D+ HK GP G GV+ E
Sbjct: 221 RELNQKRDEQDRIIYVVDGVHGFGVEDVSFADFDCDYFIAGTHKWLFGPRGTGVIIARSE 280
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.317 0.138 0.396
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 149849627
Number of Sequences: 2977
Number of extensions: 6582349
Number of successful extensions: 17090
Number of sequences better than 1.0e-10: 137
Number of HSP's better than 0.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 110
Number of HSP's that attempted gapping in prelim test: 16694
Number of HSP's gapped (non-prelim): 143
length of query: 401
length of database: 189,106,746
effective HSP length: 57
effective length of query: 344
effective length of database: 154,992,987
effective search space: 53317587528
effective search space used: 53317587528
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 166 (69.1 bits)