BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB0226 (THII) DE:THIAMIN BIOSYNTHESIS PROTEIN THII (198 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||B75149 thiamin biosynthesis protein thII PAB0226 - Pyrococc... 394 e-109 pir||B71189 hypothetical protein PH1786 - Pyrococcus horikoshii ... 326 1e-88 sp|Q58341|THII_METJA PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII... 171 5e-42 pir||T44564 probable thiamin biosynthesis protein thiI [imported... 151 5e-36 sp|O27720|THII_METTH PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII... 126 2e-28 gi|11498485 conserved hypothetical protein [Archaeoglobus fulgid... 111 8e-24 sp|Q9X220|THII_THEMA PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII... 104 6e-22 sp|O83570|THII_TREPA PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII... 103 1e-21 dbj|BAB06922.1| (AP001518) thiamin biosynthesis [Bacillus halodu... 97 2e-19 gb|AAK40666.1| Thiamine biosynthesis protein thiI (thiI) [Sulfol... 91 7e-18 sp|P47612|THII_MYCGE PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII... 90 1e-17 sp|O34595|THII_BACSU PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII... 90 2e-17 sp|P77718|THII_ECOLI THIAMINE BIOSYNTHESIS PROTEIN THII >gi|7467... 89 5e-17 sp|P55913|THII_SALTY THIAMINE BIOSYNTHESIS PROTEIN THII >gi|1135... 88 8e-17 pir||E82267 thiamin biosynthesis protein ThiI VC0894 [imported] ... 87 2e-16 pir||C82892 thiamin biosynthesis protein UU418 [imported] - Urea... 84 1e-15 emb|CAC11646.1| (AL445064) thiazole biosynthesis protein Thi I r... 84 1e-15 sp|Q9YEW8|THII_AERPE PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII... 82 5e-15 sp|P75228|THII_MYCPN PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII... 81 8e-15 gb|AAG19645.1| (AE005052) Vng1299c [Halobacterium sp. NRC-1] 79 4e-14 pir||F83005 thiazole biosynthesis protein ThiI PA5118 [imported]... 72 5e-12 >pir||B75149 thiamin biosynthesis protein thII PAB0226 - Pyrococcus abyssi (strain Orsay) >gi|5457783|emb|CAB49273.1| (AJ248284) THIAMIN BIOSYNTHESIS PROTEIN THII [Pyrococcus abyssi] Length = 198 Score = 394 bits (1002), Expect = e-109 Identities = 198/198 (100%), Positives = 198/198 (100%) Query: 1 MKVVALLSSGIDSPVAIHLVASKGAEVYPLHFKQDDVKENKVRRIVKRLQEIHGDKVKDP 60 MKVVALLSSGIDSPVAIHLVASKGAEVYPLHFKQDDVKENKVRRIVKRLQEIHGDKVKDP Sbjct: 1 MKVVALLSSGIDSPVAIHLVASKGAEVYPLHFKQDDVKENKVRRIVKRLQEIHGDKVKDP 60 Query: 61 RIVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQVAS 120 RIVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQVAS Sbjct: 61 RIVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQVAS 120 Query: 121 QTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEKEPPCPFVPRFPVVRA 180 QTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEKEPPCPFVPRFPVVRA Sbjct: 121 QTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEKEPPCPFVPRFPVVRA 180 Query: 181 GLGEFRKILNEVKDLLPK 198 GLGEFRKILNEVKDLLPK Sbjct: 181 GLGEFRKILNEVKDLLPK 198 >pir||B71189 hypothetical protein PH1786 - Pyrococcus horikoshii >gi|3258222|dbj|BAA30905.1| (AP000007) 201aa long hypothetical protein [Pyrococcus horikoshii] Length = 201 Score = 326 bits (827), Expect = 1e-88 Identities = 152/198 (76%), Positives = 182/198 (91%) Query: 1 MKVVALLSSGIDSPVAIHLVASKGAEVYPLHFKQDDVKENKVRRIVKRLQEIHGDKVKDP 60 M+V+ LLSSGIDSPVAIHL+AS+GAEVYPLHF+QD++KE K R++V RL+EIHG+KVKD Sbjct: 4 MRVIGLLSSGIDSPVAIHLMASRGAEVYPLHFRQDEIKEKKARKLVSRLKEIHGEKVKDL 63 Query: 61 RIVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQVAS 120 ++VDA +VQGPVFEKLRE+++EKW CIFCKYTMY+VA YARE+ A+AIVTGDSLGQVAS Sbjct: 64 KVVDALKVQGPVFEKLRELKREKWICIFCKYTMYMVAEKYAREVGAEAIVTGDSLGQVAS 123 Query: 121 QTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEKEPPCPFVPRFPVVRA 180 QTLDNLMV+STAT LPI+RPLIALDKEEIVRIAKEIGTF+IS E EPPCPF+P+FPVVRA Sbjct: 124 QTLDNLMVISTATNLPIIRPLIALDKEEIVRIAKEIGTFEISIENEPPCPFLPKFPVVRA 183 Query: 181 GLGEFRKILNEVKDLLPK 198 G +FRKIL E++++LPK Sbjct: 184 GFSQFRKILEEIREVLPK 201 >sp|Q58341|THII_METJA PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII >gi|2128072|pir||C64416 conserved hypothetical MG372 related protein - Methanococcus jannaschii >gi|1591602|gb|AAB98933.1| (U67536) conserved hypothetical protein [Methanococcus jannaschii] Length = 381 Score = 171 bits (429), Expect = 5e-42 Identities = 95/192 (49%), Positives = 124/192 (64%), Gaps = 2/192 (1%) Query: 2 KVVALLSSGIDSPVAIHLVASKGAEVYPLHFKQDDVKENKVRRIVKRLQEIHGDKVKDPR 61 KV+ L+S GIDSPVA ++ +G LH K + NKVR+IV+ L + D + Sbjct: 190 KVLCLISDGIDSPVAAFMMIRRGCRAVLLHLKMSEEALNKVRKIVEVLSDY--DTELEFV 247 Query: 62 IVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQVASQ 121 + D + + EKL+ I+KE +TCIFCK M VA YA+ + AIVTGD+LGQVASQ Sbjct: 248 VYDYKKDIEDIVEKLKSIKKENYTCIFCKRKMLKVAEKYAKYLDCDAIVTGDNLGQVASQ 307 Query: 122 TLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEKEPPCPFVPRFPVVRAG 181 TL NL V+S PILRPLI LDK +IV+IAKEIGT++ISTEKE CP++P+ P A Sbjct: 308 TLKNLRVISENINYPILRPLIGLDKNDIVKIAKEIGTYEISTEKEIKCPYLPKHPKTIAK 367 Query: 182 LGEFRKILNEVK 193 E +KI +VK Sbjct: 368 PEEVKKIKEKVK 379 >pir||T44564 probable thiamin biosynthesis protein thiI [imported] - Pyrococcus furiosus >gi|6066237|gb|AAF03222.1|AF156097_2 (AF156097) putative thiamine biosynthesis protein THII [Pyrococcus furiosus] Length = 363 Score = 151 bits (378), Expect = 5e-36 Identities = 88/196 (44%), Positives = 117/196 (58%), Gaps = 8/196 (4%) Query: 2 KVVALLSSGIDSPVAIHLVASKGAEVYPLHFKQDDVKENKVRRIVKRLQEIHGDKVKDPR 61 KVVAL+S GIDSPVA L+ +GAEV LHF Q +++V+++ EI D +P Sbjct: 167 KVVALISGGIDSPVAAFLMLKRGAEVIALHFDQG----TNAKKVVEKVVEILSDYSPEPI 222 Query: 62 ---IVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQV 118 + + FE+ P L +++K +WTCI CK M A A+E A IVTGDSLGQV Sbjct: 223 ELIVENHFEILKPYVSVLAKLQKREWTCILCKIAMLKRAAEIAKEEGALGIVTGDSLGQV 282 Query: 119 ASQTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTE-KEPPCPFVPRFPV 177 ASQTL NL + + + + PI RPLI DKEEIV IAK IGT+D E CPF P + Sbjct: 283 ASQTLSNLFIETISVDYPIYRPLIGFDKEEIVSIAKRIGTYDAFLEYPYCECPFRPNRVI 342 Query: 178 VRAGLGEFRKILNEVK 193 + + EF I +E++ Sbjct: 343 TQGKITEFYFIRDELR 358 >sp|O27720|THII_METTH PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII >gi|7429876|pir||C69092 conserved hypothetical protein MTH1685 - Methanobacterium thermoautotrophicum (strain Delta H) >gi|2622815|gb|AAB86157.1| (AE000926) conserved protein [Methanobacterium thermoautotrophicum] Length = 389 Score = 126 bits (314), Expect = 2e-28 Identities = 77/179 (43%), Positives = 95/179 (53%), Gaps = 8/179 (4%) Query: 2 KVVALLSSGIDSPVAIHLVASKGAEVYPLHFKQ----DDVKENKVRRIVKRLQEIHGDKV 57 KVVALLS GIDSPVA +L+ +G +V +H + E KV +I +L E Sbjct: 179 KVVALLSGGIDSPVATYLMMKRGCQVVAVHMDNAPFTGEEAEEKVEKIAAKLAEYSAGVE 238 Query: 58 KDPRIVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQ 117 R G E R EK TC+ CK+ MY +A A E A AIV G SLGQ Sbjct: 239 FKLRTFS----YGRYLESCRRGAPEKMTCVLCKFGMYHLAEMVAEEEGALAIVDGSSLGQ 294 Query: 118 VASQTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEKEPPCPFVPRFP 176 VASQTL N++ +PIL PLI +DK EI +AK IGT+DIS + C VP P Sbjct: 295 VASQTLPNILATRMGVNIPILSPLIGMDKVEIENLAKRIGTYDISVIPDGGCSAVPAHP 353 >gi|11498485 conserved hypothetical protein [Archaeoglobus fulgidus] >gi|3915121|sp|O29382|THII_ARCFU PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII >gi|7429877|pir||G69359 conserved hypothetical protein AF0879 - Archaeoglobus fulgidus >gi|2649723|gb|AAB90361.1| (AE001043) conserved hypothetical protein [Archaeoglobus fulgidus] Length = 374 Score = 111 bits (274), Expect = 8e-24 Identities = 78/191 (40%), Positives = 109/191 (56%), Gaps = 10/191 (5%) Query: 2 KVVALLSSGIDSPVAIHLVASKGAEVYPLHFKQDDVKENKVRRIVKRLQEIHGD---KVK 58 KVVAL+S GIDSPVA + +G EV +HF + KVR ++ L E + K+K Sbjct: 167 KVVALISGGIDSPVAAFMAMKRGCEVVAVHFFNQTMHSPKVREKIRMLAEKLAEYQGKIK 226 Query: 59 DPRIVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQV 118 +V EVQ + ++ K + + +M +A A + KA+VTGDSL QV Sbjct: 227 -LYMVPFKEVQFEIINRV----PPKLRMVVYRRSMMRMANLIAEKEGCKAVVTGDSLSQV 281 Query: 119 ASQTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDIS-TEKEPPCPF-VPRFP 176 ASQTLDN+ V+ +A++L +L PLI +DKEEIV IAK I T++IS E C F V + P Sbjct: 282 ASQTLDNINVIYSASKLAVLPPLIGMDKEEIVAIAKRIETYEISILPYEDCCSFMVAQHP 341 Query: 177 VVRAGLGEFRK 187 RA + + K Sbjct: 342 ETRAKIEDVVK 352 >sp|Q9X220|THII_THEMA PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII >gi|7445342|pir||H72223 thiamin biosynthesis protein ThiI - Thermotoga maritima (strain MSB8) >gi|4982270|gb|AAD36761.1|AE001809_13 (AE001809) thiamin biosynthesis protein ThiI [Thermotoga maritima] Length = 388 Score = 104 bits (258), Expect = 6e-22 Identities = 72/200 (36%), Positives = 107/200 (53%), Gaps = 10/200 (5%) Query: 2 KVVALLSSGIDSPVAIHLVASKGAEVYPLHFKQDDVKEN----KVRRIVKRLQEIHGDKV 57 K V LLS GIDSPVA +G + + F KVR I++ L+E G Sbjct: 176 KAVLLLSGGIDSPVAGWYALKRGVLIESVTFVSPPFTSEGAVEKVRDILRVLREFSGGHP 235 Query: 58 KDPRIVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQ 117 IV+ ++Q V +++ +K++ I + +M+ +A A E A A TG+++GQ Sbjct: 236 LRLHIVNLTKLQLEVKKRV----PDKYSLIMYRRSMFRIAEKIAEETGAVAFYTGENIGQ 291 Query: 118 VASQTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEK-EPPCP-FVPRF 175 VASQTL+NL + + T P++RPL DK EIV AKEIGT++IS + + C F P+ Sbjct: 292 VASQTLENLWSIESVTTRPVIRPLSGFDKTEIVEKAKEIGTYEISIKPYQDSCVFFAPKN 351 Query: 176 PVVRAGLGEFRKILNEVKDL 195 P R+ K+ +V DL Sbjct: 352 PATRSHPSILEKLEQQVPDL 371 >sp|O83570|THII_TREPA PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII >gi|7429878|pir||C71310 conserved hypothetical protein TP0559 - syphilis spirochete >gi|3322853|gb|AAC26563.1| (AE001231) conserved hypothetical protein [Treponema pallidum] Length = 401 Score = 103 bits (255), Expect = 1e-21 Identities = 68/186 (36%), Positives = 101/186 (53%), Gaps = 8/186 (4%) Query: 6 LLSSGIDSPVAIHLVASKGAEVYPLHFKQDDVKENKVRRIVKRLQEIHGDKVKDPR--IV 63 LLS GIDSPVA + + S+G + L+F + ++ V+ L ++ +V Sbjct: 186 LLSGGIDSPVAGYRMLSRGMHIDCLYFHSYPYTPPEAQKKVEDLAKVLARYGLSTTLTVV 245 Query: 64 DAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQVASQTL 123 ++Q ++L+ + + M +A ARE +A+ ++TG+SL QVASQTL Sbjct: 246 SLTDIQ----KQLQTHAPAPSLTLLLRMCMMRIAEHVAREQRARCLITGESLAQVASQTL 301 Query: 124 DNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEKEPPC--PFVPRFPVVRAG 181 +NL V S T LPI RPLI DKE+I+R A EIGT+ IS C F P+ PV+R Sbjct: 302 ENLTVTSACTHLPIFRPLIGADKEDIIRTATEIGTYAISIRPYEDCCTLFAPKHPVLRPE 361 Query: 182 LGEFRK 187 + E +K Sbjct: 362 VEEMQK 367 >dbj|BAB06922.1| (AP001518) thiamin biosynthesis [Bacillus halodurans] Length = 382 Score = 96.7 bits (237), Expect = 2e-19 Identities = 61/164 (37%), Positives = 91/164 (55%), Gaps = 8/164 (4%) Query: 2 KVVALLSSGIDSPVAIHLVASKGAEVYPLHFKQDDVKENKVRRIVKRLQEI---HGDKVK 58 KV+ +LS GIDSPVA + + +G + +HF K ++ V L G +VK Sbjct: 159 KVLLMLSGGIDSPVAGYYLLKRGVTLEAIHFHSPPFTNEKAKQKVVDLAAKLTNFGGQVK 218 Query: 59 DPRIVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQV 118 IV E+Q + EK+ + M ++ A++ A AI TG+SLGQV Sbjct: 219 -LHIVPFTELQKHIHEKV----PSNMEMTIMRRMMLRISEGIAKKTDALAIATGESLGQV 273 Query: 119 ASQTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDIS 162 ASQTL ++ ++ T P+LRPL+ +DKEE++ +AKEI TF+IS Sbjct: 274 ASQTLHSMNTINEVTTTPVLRPLLTMDKEEVITVAKEIDTFNIS 317 >gb|AAK40666.1| Thiamine biosynthesis protein thiI (thiI) [Sulfolobus solfataricus] Length = 371 Score = 91.3 bits (223), Expect = 7e-18 Identities = 65/171 (38%), Positives = 91/171 (53%), Gaps = 10/171 (5%) Query: 2 KVVALLSSGIDSPVAIHLVASKGAEVYPLHFKQD-DVKENKVRRIVKRLQEIHGD-KVKD 59 K V L S GIDSPVA ++ +G+ L+F D+ + V + + L+ G K+K Sbjct: 170 KTVVLFSGGIDSPVATWMMMKRGSVPIILNFNLGGDLHKELVLKELNMLKRWSGGHKLK- 228 Query: 60 PRIVDAFEVQGP-VFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQV 118 F V+G VF KL ++ + + K MY A + AK+I TG+SL QV Sbjct: 229 -----IFIVKGTDVFIKLSQVERRS-RVVMLKRVMYKTAEKLCEKTNAKSITTGESLSQV 282 Query: 119 ASQTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEKEPPC 169 +SQT+ NL V + PI RPLI DKEEIV IA++IGT+ S + C Sbjct: 283 SSQTMANLYVTEYGIKYPIFRPLIGFDKEEIVDIARKIGTYKHSIKLPEYC 333 >sp|P47612|THII_MYCGE PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII >gi|1361675|pir||B64241 hypothetical protein MG372 - Mycoplasma genitalium >gi|3844960|gb|AAC71599.1| (U39719) NTP-utilizing enzyme (GuaA family) [Mycoplasma genitalium] Length = 385 Score = 90.5 bits (221), Expect = 1e-17 Identities = 68/204 (33%), Positives = 107/204 (52%), Gaps = 12/204 (5%) Query: 2 KVVALLSSGIDSPVAIHLVASKGAEVYPLHFKQDDVKENK-VRRIVKRLQEIHGDKVKDP 60 K + LLS GIDSPVA LV +G + + F + K K + +I + I +K Sbjct: 179 KALVLLSGGIDSPVAASLVMQRGFNIDFITFINEPNKNQKTIEKITRLANLISFNKTICS 238 Query: 61 RIVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQVAS 120 + F+ + ++L I E + + + Y A+ + K +VTG+ LGQVAS Sbjct: 239 GKLLVFDFTA-IQKELIHISNESYRIVLMRRVFYKAASMF----KYDCLVTGEVLGQVAS 293 Query: 121 QTLDNLMVMSTAT-ELPILRPLIALDKEEIVRIAKEIGTFDISTEK--EPPCPFVPRFPV 177 QT++NL V+ +AT + I+RPLI K++I+ +AK TFDIS E+ + F P+ P Sbjct: 294 QTIENLKVIQSATPDTFIVRPLIGFSKDKIIELAKFFNTFDISIEQHLDTCSEFSPKNPT 353 Query: 178 VRAGLGEFRKILNE---VKDLLPK 198 +A L K+ +E + DL+ K Sbjct: 354 TKAKLINVEKLESELIFLNDLIEK 377 >sp|O34595|THII_BACSU PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII >gi|7429879|pir||E69988 conserved hypothetical protein ytbJ - Bacillus subtilis >gi|2293230|gb|AAC00308.1| (AF008220) YtbJ [Bacillus subtilis] >gi|2635442|emb|CAB14936.1| (Z99119) similar to hypothetical proteins [Bacillus subtilis] Length = 358 Score = 89.7 bits (219), Expect = 2e-17 Identities = 59/172 (34%), Positives = 88/172 (50%), Gaps = 10/172 (5%) Query: 2 KVVALLSSGIDSPVAIHLVASKGAEVYPLHFKQ----DDVKENKVRRIVKRLQEIHGDKV 57 K + +LS G DSPVA +G V +HF + + KV + K L G Sbjct: 179 KAMLMLSGGFDSPVAGFYAMKRGLSVEAVHFFSPPYTSERAKQKVMDLAKCLSRFGGSMT 238 Query: 58 KDPRIVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQ 117 IV + Q E +++ E +T + M +A + AI+TG+SLGQ Sbjct: 239 L--HIVPFTKTQ----ELIQKQIPENYTMTATRRLMLQIADRIREKRNGLAIITGESLGQ 292 Query: 118 VASQTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEKEPPC 169 VASQTL+++ ++ T PILRPLIA+DK EI+ ++EIGT++ S + C Sbjct: 293 VASQTLESMYAINAVTSTPILRPLIAMDKTEIIEKSREIGTYETSIQPFEDC 344 >sp|P77718|THII_ECOLI THIAMINE BIOSYNTHESIS PROTEIN THII >gi|7467031|pir||G64771 yajK protein - Escherichia coli >gi|1773107|gb|AAB40179.1| (U82664) similar to M. jannaschii MG372 [Escherichia coli] >gi|1786625|gb|AAC73526.1| (AE000148) putative oxidoreductase [Escherichia coli K12] Length = 482 Score = 88.5 bits (216), Expect = 5e-17 Identities = 58/180 (32%), Positives = 95/180 (52%), Gaps = 6/180 (3%) Query: 3 VVALLSSGIDSPVAIHLVASKGAEVYPLHFKQDDVK-ENKVRRIVKRLQEIHGDKVKDPR 61 V++L+S G DS V+ +++ +G V+ F E VR++ L G + Sbjct: 180 VLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRF 239 Query: 62 IVDAFE-VQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQVAS 120 + FE V G + EK+ + + + K M A+ A +A+VTG++LGQV+S Sbjct: 240 VAINFEPVVGEILEKIDDGQMG----VILKRMMVRAASKVAERYGVQALVTGEALGQVSS 295 Query: 121 QTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEKEPPCPFVPRFPVVRA 180 QTL NL ++ ++ ILRPLI+ DKE I+ +A++IGT D + C + + P V+A Sbjct: 296 QTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTEDFARTMPEYCGVISKSPTVKA 355 >sp|P55913|THII_SALTY THIAMINE BIOSYNTHESIS PROTEIN THII >gi|11354259|pir||T46944 thiamin biosynthesis protein thiI [validated] - Salmonella typhimurium >gi|2098763|gb|AAB63031.1| (U94901) ThiI [Salmonella typhimurium] Length = 482 Score = 87.8 bits (214), Expect = 8e-17 Identities = 58/180 (32%), Positives = 95/180 (52%), Gaps = 6/180 (3%) Query: 3 VVALLSSGIDSPVAIHLVASKGAEVYPLHFKQDDVK-ENKVRRIVKRLQEIHGDKVKDPR 61 V++L+S G DS V+ +++ +G V+ F E VR++ L G + Sbjct: 180 VLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRF 239 Query: 62 IVDAFE-VQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQVAS 120 + FE V G + EK+ + + + K M A+ A +A+VTG++LGQV+S Sbjct: 240 VAINFEPVVGEILEKVDDGQMG----VVLKRMMVRAASKVAERYGVQALVTGEALGQVSS 295 Query: 121 QTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEKEPPCPFVPRFPVVRA 180 QTL NL ++ ++ ILRPLI+ DKE I+ +A++IGT D + C + + P V+A Sbjct: 296 QTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTEDFARTMPEYCGVISKSPTVKA 355 >pir||E82267 thiamin biosynthesis protein ThiI VC0894 [imported] - Vibrio cholerae (group O1 strain N16961) >gi|9655349|gb|AAF94056.1| (AE004173) thiamin biosynthesis protein ThiI [Vibrio cholerae] Length = 515 Score = 86.6 bits (211), Expect = 2e-16 Identities = 60/179 (33%), Positives = 93/179 (51%), Gaps = 4/179 (2%) Query: 3 VVALLSSGIDSPVAIHLVASKGAEVYPLHFKQDD-VKENKVRRIVKRLQEIHGDKVKDPR 61 V++L+S G DS V+ +L +G++V+ F E V++ L + +G K Sbjct: 212 VLSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQTAYYLWQKYGSSAKVRF 271 Query: 62 IVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQVASQ 121 I F PV ++ E + + K A A + +A+VTG++LGQV+SQ Sbjct: 272 IAIDF---APVVAEILEKIDDGQMGVVLKRMFMRTAGMVAEKFGIQALVTGEALGQVSSQ 328 Query: 122 TLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEKEPPCPFVPRFPVVRA 180 TL NL + T+ ILRPLI DKE+I+R+A+EIGT D + C + + P V+A Sbjct: 329 TLTNLRHIDNVTDTLILRPLINWDKEDIIRLAREIGTEDFAKTMPEFCGVISKSPTVKA 387 >pir||C82892 thiamin biosynthesis protein UU418 [imported] - Ureaplasma urealyticum >gi|6899407|gb|AAF30829.1|AE002139_2 (AE002139) thiamin biosynthesis protein [Ureaplasma urealyticum] Length = 390 Score = 83.9 bits (204), Expect = 1e-15 Identities = 61/199 (30%), Positives = 102/199 (50%), Gaps = 15/199 (7%) Query: 2 KVVALLSSGIDSPVAIHLVASKGAEV------YPLHFKQDDVKENKVRRIVKRLQEIHGD 55 K + LLS GIDSPVA L+ +G + P H Q + +K + K++ + Sbjct: 176 KALVLLSGGIDSPVASRLIMKRGISIDFITFITPPHTSQKAL--DKTIALAKQITLDNKL 233 Query: 56 KVKDPRIVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSL 115 + + + ++Q +++ I KE + + +A A +KA A+VTG++L Sbjct: 234 TKANLYVCNFTKLQ----DEIAHISKESYRITLMRRYFMRIAKRLAISVKANALVTGEAL 289 Query: 116 GQVASQTLDNLMVMSTATE-LPILRPLIALDKEEIVRIAKEIGTFDIS-TEKEPPCP-FV 172 GQVASQTL+++ +S+ + +LRPLIA DKEEI+ +AK T+++S + C F Sbjct: 290 GQVASQTLNSMQTISSVLDNFLVLRPLIAYDKEEIISLAKRFNTYELSILPYDDSCSLFA 349 Query: 173 PRFPVVRAGLGEFRKILNE 191 P+ P + K+ E Sbjct: 350 PKNPTTNPNVQTAAKLEEE 368 >emb|CAC11646.1| (AL445064) thiazole biosynthesis protein Thi I related protein [Thermoplasma acidophilum] Length = 469 Score = 83.9 bits (204), Expect = 1e-15 Identities = 63/190 (33%), Positives = 100/190 (52%), Gaps = 25/190 (13%) Query: 2 KVVALLSSGIDSPVAIHLVASKGAEV--------YPLHFKQ--DDVKENKVRRIVKRLQE 51 K ++L+S GIDSPV+ ++ +G+ YP+ + D VK+ R R Sbjct: 158 KYISLVSGGIDSPVSTWMIMKRGSPCDILFCSLSYPVDLRPFVDVVKKLVERWAPYRKPR 217 Query: 52 IHGDKVKDPR-IVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIV 110 I+ V D R ++ ++G + K++ + K +Y +A A E IV Sbjct: 218 IY---VADCRSLIRTMVIEG----------RTKYSNVTFKRVIYRIAEKIALENGYNGIV 264 Query: 111 TGDSLGQVASQTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEKEPPCP 170 TG+SLGQV+SQT +NL + + +PILRPLI +DK+E+V +A+ IGTF + E Sbjct: 265 TGESLGQVSSQTAENLKAIESGIGVPILRPLIGMDKDEVVDMARRIGTFPELSMGEFCSL 324 Query: 171 FVPRFPVVRA 180 F R P++R+ Sbjct: 325 FASR-PIIRS 333 >sp|Q9YEW8|THII_AERPE PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII >gi|7445341|pir||H72741 probable thiazole biosynthesis gene thi I APE0465 - Aeropyrum pernix (strain K1) >gi|5104112|dbj|BAA79428.1| (AP000059) 412aa long hypothetical thiazole biosynthesis gene thi I [Aeropyrum pernix] Length = 412 Score = 81.9 bits (199), Expect = 5e-15 Identities = 64/194 (32%), Positives = 103/194 (52%), Gaps = 7/194 (3%) Query: 4 VALLSSGIDSPVAIHLVASKGAEVYPLHFKQDDVKENKVRRIVKRLQEIHGDKVKD--PR 61 +AL S G DS VA ++ +G+ V+ L F V E + V+ + + G+ PR Sbjct: 198 LALYSGGFDSGVAAWMIMRRGSPVH-LAFYDFGVPE-ALEVAVEGAKTLAGEWAWGYRPR 255 Query: 62 IVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQVASQ 121 + +G + + K + + + M + A A +A+VTG+S+GQVASQ Sbjct: 256 LY-VVNFRGAALI-VNGLVKPSYRTLVLRRLMLLHAQDLAAREGFEALVTGESVGQVASQ 313 Query: 122 TLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEKEPPCPFVPRFPVVRAG 181 T+ NL ++S+ ELP+LRPL +DK+EIV ++EIG +DI+ + C V + P RA Sbjct: 314 TVRNLRLISSGLELPVLRPLAGMDKDEIVEKSREIGLYDIARRQVEVCG-VDQPPNPRAS 372 Query: 182 LGEFRKILNEVKDL 195 FR +V+D+ Sbjct: 373 PQGFRSEFEKVRDI 386 >sp|P75228|THII_MYCPN PROBABLE THIAMINE BIOSYNTHESIS PROTEIN THII >gi|2146443|pir||S73618 MG372 homolog G12_orf387 - Mycoplasma pneumoniae (strain ATCC 29342) >gi|1673963|gb|AAB95940.1| (AE000027) Thiamin biosynthesis protein [Mycoplasma pneumoniae] Length = 387 Score = 81.1 bits (197), Expect = 8e-15 Identities = 62/199 (31%), Positives = 102/199 (51%), Gaps = 21/199 (10%) Query: 4 VALLSSGIDSPVAIHLVASKGAEV-------YPLHFKQDDVKENKVRRIVKRLQEIHGDK 56 +ALLS GIDSPVA LV +G V P H K ++ +V Q + + Sbjct: 183 LALLSGGIDSPVAASLVLQRGFNVDFITFINEPGHNAATIGKIQRLANLVSLNQTLCTGR 242 Query: 57 VKDPRIVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLG 116 + + D ++Q ++L I E + + + Y +A+ + K ++TG++LG Sbjct: 243 LF---VFDFTDLQ----KELSHISLEGYRIVLMRRCFYKIASLF----KYDCLITGEALG 291 Query: 117 QVASQTLDNL-MVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTE--KEPPCPFVP 173 QVASQT+DNL ++ + ++RPLI L K++I+ AK +GTF+ S E + F P Sbjct: 292 QVASQTIDNLKVIQAVVPNTFVIRPLIGLSKDKIIEWAKALGTFETSIEHHMDTCTVFAP 351 Query: 174 RFPVVRAGLGEFRKILNEV 192 + P +A L K+ +E+ Sbjct: 352 KKPTTKAKLAIVEKLESEL 370 >gb|AAG19645.1| (AE005052) Vng1299c [Halobacterium sp. NRC-1] Length = 392 Score = 78.8 bits (191), Expect = 4e-14 Identities = 62/188 (32%), Positives = 87/188 (45%), Gaps = 9/188 (4%) Query: 3 VVALLSSGIDSPVAIHLVASKGAEVYPLHFKQDDV----KENKVRRIVKRLQEIHGDKVK 58 +VAL+S GIDSPVA +GA V PL+ D + V+ L + + Sbjct: 184 LVALVSGGIDSPVAAWESMRRGAPVIPLYLALGDYGGPDHRARAEAAVRTLDDYAPNHDL 243 Query: 59 DPRIVDAFEVQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQV 118 R+ A G ++L F ++ MY VA A A IVTG+++GQ Sbjct: 244 SLRVAPA----GDAIDRLAAATGRTRMLSFRRF-MYRVAEHVAEHAGAAGIVTGEAVGQK 298 Query: 119 ASQTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEKEPPCPFVPRFPVV 178 +SQT NL V+ AT LP+ RPL+ DK+ I A+ I TF S+ PR P+ Sbjct: 299 SSQTTANLGVVDRATTLPVHRPLLTWDKQRITAAARSIDTFRDSSLDVGCNRLAPRQPLT 358 Query: 179 RAGLGEFR 186 A + R Sbjct: 359 AAPIESVR 366 >pir||F83005 thiazole biosynthesis protein ThiI PA5118 [imported] - Pseudomonas aeruginosa (strain PAO1) >gi|9951416|gb|AAG08503.1|AE004925_1 (AE004925) thiazole biosynthesis protein ThiI [Pseudomonas aeruginosa] Length = 484 Score = 71.8 bits (173), Expect = 5e-12 Identities = 52/181 (28%), Positives = 89/181 (48%), Gaps = 6/181 (3%) Query: 2 KVVALLSSGIDSPVAIHLVASKGAEVYPLHFKQDD-VKENKVRRIVKRLQEIHGDKVKDP 60 +V+ L+S G DS VA + + +G + + F E V + L E +G + Sbjct: 181 QVLVLMSGGFDSTVAAYQMMRRGMISHFVFFNLGGRAHELGVMEVAHYLWEKYGRSQRVL 240 Query: 61 RIVDAFE-VQGPVFEKLREIRKEKWTCIFCKYTMYIVATSYAREIKAKAIVTGDSLGQVA 119 + FE V G + K+ + + + K M A+ A ++ A+VTG+++ QV+ Sbjct: 241 FVSVPFEEVVGEILTKV----DDSYMGVTLKRMMLRAASRVAERLELDALVTGEAISQVS 296 Query: 120 SQTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDISTEKEPPCPFVPRFPVVR 179 SQTL NL V+ T+ +LRPLI K++I+ A++IGT + + C + P + Sbjct: 297 SQTLPNLSVIDRVTDTLVLRPLIVSHKQDIIDTARQIGTAEFARHMPEYCGVISVNPTTQ 356 Query: 180 A 180 A Sbjct: 357 A 357 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.321 0.139 0.395 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 68120407 Number of Sequences: 2977 Number of extensions: 2617130 Number of successful extensions: 8659 Number of sequences better than 1.0e-10: 21 Number of HSP's better than 0.0 without gapping: 16 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 8626 Number of HSP's gapped (non-prelim): 21 length of query: 198 length of database: 189,106,746 effective HSP length: 55 effective length of query: 143 effective length of database: 156,189,961 effective search space: 22335164423 effective search space used: 22335164423 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.8 bits) S2: 163 (67.9 bits)