BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0253 (ksgA) DE:dimethyladenosine transferase (ksgA)
(269 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||F75152 dimethyladenosine transferase (ksga) PAB0253 - Pyroc... 529 e-149
pir||G71193 probable dimethyladenosine transferase - Pyrococcus ... 429 e-119
pir||C69043 conserved hypothetical protein MTH1326 - Methanobact... 221 6e-57
pir||D64428 probable (adenine-N6,N6-)-dimethyltransferase (EC 2.... 213 2e-54
gi|7657198 putative dimethyladenosine transferase [Homo sapiens]... 168 9e-41
pir||T00442 rRNA (adenine-N6,N6-)-dimethyltransferase homolog T3... 167 1e-40
gb|AAG19544.1| (AE005045) dimethyladenosine transferase; KsgA [H... 161 9e-39
gb|AAF56847.1| (AE003768) CG11837 gene product [Drosophila melan... 152 3e-36
sp|Q09522|YQN1_CAEEL HYPOTHETICAL 34.1 KD PROTEIN E02H1.1 IN CHR... 150 1e-35
dbj|BAB10912.1| (AB013389) dimethyladenosine transferase-like pr... 147 1e-34
gi|11499372 dimethyladenosine transferase (ksgA) [Archaeoglobus ... 145 7e-34
pir||T40240 dimethylase - fission yeast (Schizosaccharomyces pom... 140 1e-32
sp|P37468|KSGA_BACSU DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLME... 139 2e-32
sp|P78697|DIM1_KLULA DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLME... 139 3e-32
gi|6324989 dimethyladenosine transferase; Dim1p [Saccharomyces c... 138 5e-32
pir||T43249 rRNA (adenine-N6,N6-)-dimethyltransferase (EC 2.1.1.... 138 7e-32
dbj|BAB03776.1| (AP001507) dimethyladenosine transferase [Bacill... 136 2e-31
pir||B72255 dimethyladenosine transferase - Thermotoga maritima ... 132 4e-30
pir||T09625 rRNA (adenine-N6,N6-)-dimethyltransferase (EC 2.1.1.... 128 8e-29
sp|P43038|KSGA_MYCCA DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLME... 127 2e-28
pir||H81168 dimethyladenosine transferase NMB0697 [imported] - N... 124 9e-28
pir||F81936 probable dimethyladenosine transferase (EC 2.1.1.-) ... 124 1e-27
pir||E70173 dimethyladenosine transferase (ksgA) homolog - Lyme ... 120 2e-26
pir||H83571 rRNA (adenine-N6,N6)-dimethyltransferase PA0592 [imp... 119 4e-26
sp|O05952|KSGA_RICPR DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLME... 118 5e-26
pir||F75385 dimethyladenosine transferase - Deinococcus radiodur... 118 5e-26
pir||D81680 dimethyladenosine transferase TC0633 [imported] - Ch... 115 8e-25
pir||H72001 dimethyladenosine transferase CP0791 [imported] - Ch... 114 1e-24
pir||B82323 dimethyladenosine transferase VC0443 [imported] - Vi... 112 4e-24
pir||C71526 probable dimethyladenosine transferase - Chlamydia t... 111 1e-23
pir||E82871 dimethyladenosine transferase UU601 [imported] - Ure... 111 1e-23
pir||S74516 (adenine-N6,N6-)-dimethyltransferase (EC 2.1.1.-) ks... 110 2e-23
emb|CAB95942.1| (AL359989) putative dimethyladenosine transferas... 109 4e-23
sp|P47701|KSGA_MYCGE DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLME... 107 1e-22
sp|P75113|KSGA_MYCPN DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLME... 107 2e-22
sp|P57241|KSGA_BUCAI DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLME... 106 2e-22
pir||A47697 probable methyltransferase (EC 2.1.1.-) - Bacillus a... 104 9e-22
sp|Q04720|ERMJ_BACAN RRNA ADENINE N-6-METHYLTRANSFERASE (MACROLI... 104 1e-21
sp|Q03986|ERMD_BACLI RRNA ADENINE N-6-METHYLTRANSFERASE (MACROLI... 104 1e-21
dbj|BAA83962.1| (AB024564) ERMK [Bacillus halodurans] >gi|101729... 104 1e-21
emb|CAB64788.1| (AJ242786) KsgA, high level kasugamycin resistan... 104 1e-21
pir||A72640 probable dimethyladenosine transferase APE0553 - Aer... 103 2e-21
pir||H82593 dimethyladenosine transferase XF2148 [imported] - Xy... 102 4e-21
sp|P45438|ERMK_BACLI RRNA ADENINE N-6-METHYLTRANSFERASE (MACROLI... 101 7e-21
pir||T51591 dimethyladenosine transferase (EC 2.1.1.-) PFC1 [val... 101 7e-21
emb|CAC12422.1| (AL445067) rRNA (adenine-N6, N6-)-dimethyltransf... 101 1e-20
sp|P44749|KSGA_HAEIN DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLME... 99 5e-20
emb|CAB60001.1| (X51891) N-6-aminoadenine-N-methyltransferase [S... 97 2e-19
sp|P06992|KSGA_ECOLI DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLME... 97 3e-19
sp|P06571|ERMG_BACSH RRNA ADENINE N-6-METHYLTRANSFERASE (MACROLI... 96 3e-19
gi|11416997 putative dimethyladenosine transferase [Homo sapiens] 95 1e-18
gb|AAC37034.1| (L42817) rRNA methyltransferase [Bacteroides thet... 94 2e-18
pir||F70456 dimethyladenosine transferase - Aquifex aeolicus >gi... 93 3e-18
sp|P10337|ERMF_BACFR RRNA ADENINE N-6-METHYLTRANSFERASE (MACROLI... 93 4e-18
gb|AAA27431.1| (M17808) clindamycin resistance determinant (ermF... 93 4e-18
pir||T29195 hypothetical protein T03F1.7 - Caenorhabditis elegan... 93 4e-18
gb|AAF68230.1|AF203972_3 (AF203972) Shuttle vector pBA complete ... 93 4e-18
sp|Q02607|ERMU_BACFR RRNA ADENINE N-6-METHYLTRANSFERASE (MACROLI... 91 1e-17
pir||G71337 probable dimethyladenosine transferase (ksgA) - syph... 90 2e-17
gb|AAC36915.1| (L22689) 23S rRNA methlyase [Clostridium perfring... 90 3e-17
pir||C81269 probable dimethyladenosine transferase (16S rRNA dim... 87 2e-16
gi|9507344 adenine methylase (ermC) [Plasmid pE194] >gi|127193|s... 85 8e-16
gb|AAD46055.1|AF076212_5 (AF076212) adenine methylase [Promoter ... 85 8e-16
pir||E70603 probable dimethyladenosine transferase - Mycobacteri... 84 1e-15
gi|7705785 CGI-75 protein [Homo sapiens] >gi|4929619|gb|AAD34070... 84 2e-15
gi|11417701 similar to CGI-75 protein (H. sapiens) [Homo sapiens... 84 2e-15
gb|AAG37071.1|AF310974_2 (AF310974) methylase ermT [Plasmid p121BS] 84 2e-15
emb|CAA70207.1| (Y09002) adenine methylase [Staphylococcus haemo... 83 2e-15
gi|9507389 23S RNA methylase [Plasmid pT48] >gi|127195|sp|P13978... 83 4e-15
pir||S42117 kasugamycin dimethyltransferase (EC 2.1.1.-) - Mycop... 83 4e-15
gi|9507309 macrolide-lincosamide-streptogramin B-resistance prot... 82 7e-15
emb|CAA70208.1| (Y09003) adenine methylase [Staphylococcus hyicus] 82 7e-15
sp|P07287|ERME_SACER RRNA ADENINE N-6-METHYLTRANSFERASE (MACROLI... 82 9e-15
prf||1112175A transferase,N-Me [Saccharopolyspora erythraea] 82 9e-15
sp|P13079|CARB_STRTH RRNA METHYLTRANSFERASE (CARBOMYCIN-RESISTAN... 81 1e-14
sp|Q00014|ERMG_LACRE RRNA ADENINE N-6-METHYLTRANSFERASE (MACROLI... 81 1e-14
sp|P13957|ERM3_STAAU RRNA ADENINE N-6-METHYLTRANSFERASE (MACROLI... 81 2e-14
gi|9507371 macrolide-lincosamide-streptogramin B-resistance prot... 80 2e-14
gb|AAB60941.1| (AF002716) erythromycin resistance methylase [Str... 80 2e-14
gb|AAC45552.1| (U82607) rRNA N-6-methyltransferase [Staphylococc... 79 5e-14
sp|P06699|ERM1_STAAU RRNA ADENINE N-6-METHYLTRANSFERASE (MACROLI... 78 8e-14
gb|AAC33146.1| (AF019140) rRNA N-6-methyltransferase [Staphyloco... 78 8e-14
gb|AAA26492.1| (M11200) N-6-amino adenine-N-methyl transferase [... 78 1e-13
emb|CAA70206.1| (Y09001) adenine methylase [Staphylococcus hominis] 78 1e-13
pir||S68607 protein Erm2 - Streptococcus pyogenes plasmid pBT233... 77 2e-13
sp|P20173|ERM2_ENTFA RRNA ADENINE N-6-METHYLTRANSFERASE (MACROLI... 77 2e-13
pir||S45087 probable MSL methylase erm2 - Streptococcus pyogenes... 77 2e-13
gi|10956213 MSL methylase [Streptococcus agalactiae] >gi|1363996... 76 3e-13
gb|AAC45607.1| (U48430) rRNA methylase [Lactobacillus fermentum] 76 3e-13
gb|AAB86539.1| (AF030064) erythromycin resistance determinant [L... 76 3e-13
gb|AAC31204.1| (AF080450) rRNA methylase [Lactobacillus reuteri] 76 3e-13
pir||B27739 erythromycin resistance protein ermBC - Escherichia ... 76 4e-13
sp|P10738|ERMB_ECOLI RRNA ADENINE N-6-METHYLTRANSFERASE (MACROLI... 76 5e-13
sp|P06573|ERM_STRSA RRNA ADENINE N-6-METHYLTRANSFERASE (MACROLID... 76 5e-13
gb|AAD37840.1|AF109075_2 (AF109075) rRNA methyltransferase ErmBZ... 76 5e-13
sp|P12038|ERM1_ENTFA RRNA ADENINE N-6-METHYLTRANSFERASE (MACROLI... 75 7e-13
pir||A27507 DNA adenine methylase (EC 2.1.1.-), ORF2 and ORF3 - ... 75 7e-13
sp|P21236|ERM_STRPN RRNA ADENINE N-6-METHYLTRANSFERASE (MACROLID... 75 7e-13
gb|AAG09761.1| (AF239772) erythromycin resistance methylase [Sta... 75 7e-13
pir||S49316 adenine methylase (EC 2.1.1.-) - Enterococcus hirae 75 9e-13
emb|CAA57314.1| (X81655) adenine methylase [Enterococcus hirae] 75 9e-13
emb|CAA08815.1| (AJ009761) putative dimethyladenosine transferas... 75 9e-13
gb|AAF64431.1|AF229200_4 (AF229200) erythromycin resistance meth... 75 1e-12
sp|P09891|ERMA_ARTS3 RRNA ADENINE N-6-METHYLTRANSFERASE (MACROLI... 74 2e-12
gb|AAA73396.1| (U00453) adenine methylase [Streptococcus agalact... 74 2e-12
pir||E71821 dimethyladenosine transferase - Helicobacter pylori ... 74 2e-12
gb|AAF76480.1|AC020622_14 (AC020622) Identical to dimethyladenos... 73 5e-12
pir||G64698 16S rRNA (adenosine-N6,N6-)-dimethyltransferase (EC ... 71 1e-11
sp|P43433|MYRB_MICGR MYCINAMICIN-RESISTANCE PROTEIN MYRB >gi|286... 71 2e-11
>pir||F75152 dimethyladenosine transferase (ksga) PAB0253 - Pyrococcus abyssi
(strain Orsay) >gi|5457811|emb|CAB49301.1| (AJ248284)
dimethyladenosine transferase (ksgA) [Pyrococcus abyssi]
Length = 269
Score = 529 bits (1348), Expect = e-149
Identities = 269/269 (100%), Positives = 269/269 (100%)
Query: 1 MRDRLFFLLSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDEL 60
MRDRLFFLLSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDEL
Sbjct: 1 MRDRLFFLLSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDEL 60
Query: 61 SKRAKKVFTIELDRRIIEILRNEYSWNNVEIIQGDAVKVEWPSFNKVVSNIPYQISSPFT 120
SKRAKKVFTIELDRRIIEILRNEYSWNNVEIIQGDAVKVEWPSFNKVVSNIPYQISSPFT
Sbjct: 61 SKRAKKVFTIELDRRIIEILRNEYSWNNVEIIQGDAVKVEWPSFNKVVSNIPYQISSPFT 120
Query: 121 FKLLKMEFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKP 180
FKLLKMEFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKP
Sbjct: 121 FKLLKMEFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKP 180
Query: 181 KVDSALVLITPKKDRIELNESLVKALFQHRRKVVSKALRESAHMLGIKDVKTVKDILSSV 240
KVDSALVLITPKKDRIELNESLVKALFQHRRKVVSKALRESAHMLGIKDVKTVKDILSSV
Sbjct: 181 KVDSALVLITPKKDRIELNESLVKALFQHRRKVVSKALRESAHMLGIKDVKTVKDILSSV 240
Query: 241 PHSNKRVFHLTPEEVKEIEEYLREHRIIS 269
PHSNKRVFHLTPEEVKEIEEYLREHRIIS
Sbjct: 241 PHSNKRVFHLTPEEVKEIEEYLREHRIIS 269
>pir||G71193 probable dimethyladenosine transferase - Pyrococcus horikoshii
>gi|3258259|dbj|BAA30942.1| (AP000007) 290aa long
hypothetical dimethyladenosine transferase [Pyrococcus
horikoshii]
Length = 290
Score = 429 bits (1091), Expect = e-119
Identities = 209/269 (77%), Positives = 244/269 (90%), Gaps = 1/269 (0%)
Query: 1 MRDRLFFLLSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDEL 60
MRDRLFFLLSKYGIRP D IGQHFLI++DVI+KAIE A V+++DV+LEVGPGLGFLTDEL
Sbjct: 23 MRDRLFFLLSKYGIRPRDSIGQHFLIIEDVIEKAIETANVNENDVILEVGPGLGFLTDEL 82
Query: 61 SKRAKKVFTIELDRRIIEILRNEYSWNNVEIIQGDAVKVEWPSFNKVVSNIPYQISSPFT 120
+KRAKKV+TIE+D++IIEIL+ EYSWNNV+IIQGDAV+VEWP FNKVVSNIPY+ISSPFT
Sbjct: 83 AKRAKKVYTIEIDQKIIEILKKEYSWNNVKIIQGDAVRVEWPKFNKVVSNIPYKISSPFT 142
Query: 121 FKLLKMEFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKP 180
FKLLK +FERAVVMYQLEFALRM AKPG RNYSRLSLM QAL +VEIVM+IGKGAFYP+P
Sbjct: 143 FKLLKTDFERAVVMYQLEFALRMVAKPGSRNYSRLSLMAQALGNVEIVMKIGKGAFYPRP 202
Query: 181 KVDSALVLITPKKDRIELNESLVKALFQHRRKVVSKALRESAHMLGIKDVKTVKDILSSV 240
KVDSALVLI P+KD+I LNE+LVKALFQHRRK V +AL++S HMLG+ ++ I+++V
Sbjct: 203 KVDSALVLIEPRKDKIVLNENLVKALFQHRRKTVPRALKDSIHMLGVSK-DEIRGIINNV 261
Query: 241 PHSNKRVFHLTPEEVKEIEEYLREHRIIS 269
PHSNKRVF L PEEVK+IEEYL++H IIS
Sbjct: 262 PHSNKRVFQLYPEEVKDIEEYLKKHGIIS 290
>pir||C69043 conserved hypothetical protein MTH1326 - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2622432|gb|AAB85804.1| (AE000896) dimethyladenosine
transferase [Methanobacterium thermoautotrophicum]
Length = 273
Score = 221 bits (558), Expect = 6e-57
Identities = 121/260 (46%), Positives = 174/260 (66%), Gaps = 5/260 (1%)
Query: 8 LLSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKV 67
+L KYG+R +GQ++LI + + +E A++ + D VLE+GPG+G LT +++ A V
Sbjct: 11 VLRKYGVRLRRSLGQNYLIDEVKRQRILEYADLREDDRVLEIGPGIGTLTLPMAELAGHV 70
Query: 68 FTIELDRRIIEILRNEYSWNNVEIIQGDAVKVEWPSFNKVVSNIPYQISSPFTFKLLKME 127
IE D I IL + +NV++I GDA++V++P FNKVVSN+PYQISSP TF+LL+ +
Sbjct: 71 TAIESDPLIAAILMDRLQVDNVDVIVGDALRVDFPEFNKVVSNLPYQISSPITFRLLEHD 130
Query: 128 FERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALV 187
FE AV+MYQ EFA RM A+PG R YSRLS+M LA+VEIV + G F+P+P+V+SA+V
Sbjct: 131 FELAVLMYQKEFARRMVAEPGTREYSRLSVMVHFLAEVEIVDYLKPGCFFPRPRVESAVV 190
Query: 188 LITPKKDRI-ELNESLVKALFQHRRKVVSKALRESAHMLGIKDVKTVKDILSSVPHS--N 244
+ P R E + +ALFQHR+K SK+LRES H I+ + ++L +P
Sbjct: 191 TLKPTGFRAPAFLEDVCRALFQHRKKKTSKSLRESFH--EIRTDLSFNEVLRGLPPEILE 248
Query: 245 KRVFHLTPEEVKEIEEYLRE 264
KRVF L PE++ EI E++ +
Sbjct: 249 KRVFQLRPEDILEIAEHIED 268
>pir||D64428 probable (adenine-N6,N6-)-dimethyltransferase (EC 2.1.1.-) -
Methanococcus jannaschii >gi|1591684|gb|AAB99033.1|
(U67545) dimethyladenosine transferase (ksgA)
[Methanococcus jannaschii]
Length = 275
Score = 213 bits (537), Expect = 2e-54
Identities = 120/250 (48%), Positives = 168/250 (67%), Gaps = 11/250 (4%)
Query: 15 RPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDR 74
+P ++GQ FLI K+ ++KA+E A ++K DVVLE+G G G LT+EL+K AKKV+ IE+D+
Sbjct: 3 KPKKKLGQCFLIDKNFVNKAVESANLTKDDVVLEIGLGKGILTEELAKNAKKVYVIEIDK 62
Query: 75 RIIEILRNEYS--WNNVEIIQGDAVKVEWPS--FNKVVSNIPYQISSPFTFKLLKMEFER 130
+ E N+ +NN+EII GDA+KV+ FNKVV+N+PYQISSP TFKL+K F+
Sbjct: 63 SL-EPYANKLKELYNNIEIIWGDALKVDLNKLDFNKVVANLPYQISSPITFKLIKRGFDL 121
Query: 131 AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
AV+MYQ EFA RM AK G ++Y RLS+ Q+ ADVEIV ++ AFYPKPKV SA+V I
Sbjct: 122 AVLMYQYEFAKRMVAKEGTKDYGRLSVAVQSRADVEIVAKVPPSAFYPKPKVYSAIVKIK 181
Query: 191 PKKDRIELN-----ESLVKALFQHRRKVVSKALRESAHMLGIKDVKTVKDILSSVPHSNK 245
P K + + + ++A+FQHR K V KAL +S+ L + +K IL ++N
Sbjct: 182 PNKGKYHIENENFFDDFLRAIFQHRNKSVRKALIDSSKELNY-NKDEMKKILEDFLNTNS 240
Query: 246 RVFHLTPEEV 255
+ +L E+V
Sbjct: 241 EIKNLINEKV 250
>gi|7657198 putative dimethyladenosine transferase [Homo sapiens]
>gi|4050050|gb|AAC97955.1| (AF102147) putative
dimethyladenosine transferase [Homo sapiens]
Length = 313
Score = 168 bits (420), Expect = 9e-41
Identities = 98/225 (43%), Positives = 138/225 (60%), Gaps = 10/225 (4%)
Query: 8 LLSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKV 67
L S G+ N IGQH L +I+ I+ A + +DVVLEVGPG G +T +L ++AKKV
Sbjct: 22 LKSAGGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKV 81
Query: 68 FTIELDRRIIEILRNEYSWNNV----EIIQGDAVKVEWPSFNKVVSNIPYQISSPFTFKL 123
ELD R++ L V +++ GD +K + P F+ V+N+PYQISSPF FKL
Sbjct: 82 VACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCVANLPYQISSPFVFKL 141
Query: 124 L--KMEFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPK 181
L + F A++M+Q EFALR+ AKPGD+ Y RLS+ TQ LA V+ +M++GK F P PK
Sbjct: 142 LLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPK 201
Query: 182 VDSALVLITPKKDRIELN----ESLVKALFQHRRKVVSKALRESA 222
V+S++V I PK +N + LV+ F + K +S A + SA
Sbjct: 202 VESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSA 246
>pir||T00442 rRNA (adenine-N6,N6-)-dimethyltransferase homolog T30B22.28 -
Arabidopsis thaliana >gi|2529685|gb|AAC62868.1|
(AC002535) putative dimethyladenosine transferase
[Arabidopsis thaliana]
Length = 353
Score = 167 bits (419), Expect = 1e-40
Identities = 93/250 (37%), Positives = 151/250 (60%), Gaps = 11/250 (4%)
Query: 13 GIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIEL 72
GI + GQH L ++D ++ A + +DV+LE+GPG G LT +L + K+V +EL
Sbjct: 25 GISFHKSKGQHILKNPLLVDSIVQKAGIKSTDVILEIGPGTGNLTKKLLEAGKEVIAVEL 84
Query: 73 DRRIIEILRNEYSW----NNVEIIQGDAVKVEWPSFNKVVSNIPYQISSPFTFKLL--KM 126
D R++ L+ + N +++IQGD +K E P F+ V+NIPYQISSP TFKLL
Sbjct: 85 DSRMVLELQRRFQGTPFSNRLKVIQGDVLKTELPRFDICVANIPYQISSPLTFKLLFHPT 144
Query: 127 EFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSAL 186
F AV+MYQ EFA+R+ A+PGD Y RLS+ TQ A V ++++GK F P PKVDS++
Sbjct: 145 SFRCAVIMYQREFAMRLVAQPGDNLYCRLSVNTQLYARVSHLLKVGKNNFRPPPKVDSSV 204
Query: 187 VLITPKKDRIELN----ESLVKALFQHRRKVVSKALRESAHMLGI-KDVKTVKDILSSVP 241
V I P++ ++N + ++ F + K + ++ + + + K+ KT++ +L+S+
Sbjct: 205 VRIEPRRPGPQVNKKEWDGFLRVCFIRKNKTLGSIFKQKSVLSMLEKNFKTLQAVLASLQ 264
Query: 242 HSNKRVFHLT 251
++ + + T
Sbjct: 265 NNGEPALNTT 274
>gb|AAG19544.1| (AE005045) dimethyladenosine transferase; KsgA [Halobacterium sp.
NRC-1]
Length = 282
Score = 161 bits (403), Expect = 9e-39
Identities = 100/243 (41%), Positives = 138/243 (56%), Gaps = 15/243 (6%)
Query: 15 RPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDR 74
RPN QHFLI V+D+ A+ VLE+G G G LTD L A +V +E D
Sbjct: 16 RPNPDFDQHFLIDDRVLDRIPTYADGFDRGHVLEIGAGTGALTDRLLSVADRVTAVERDE 75
Query: 75 RIIEILRNEYS----WNNVEIIQGDAVKVEWPSFNKVVSNIPYQISSPFTFKLLKMEFER 130
LR E++ +++++ GDA+ V+ P+F VSN+PY ++S TF+LL +
Sbjct: 76 SYASFLREEFADAIAAGDLDVVAGDALAVDLPAFTCAVSNLPYGVASEVTFRLLPAG-KP 134
Query: 131 AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
V+MYQLEFA RM A PG Y RLS+ TQ ADV IV + AF P+P+V+SA+V +T
Sbjct: 135 MVLMYQLEFAERMAADPGTSEYGRLSVATQHYADVSIVETVPAAAFDPQPRVESAVVRVT 194
Query: 191 PK-KDRIELNE----SLVKALFQHRRKVVSKALRESAHMLGIKDVKTV-----KDILSSV 240
P+ D + +E S VKALF RRK A+R +AH+ G+ D V D+L +
Sbjct: 195 PRDPDYVVADEAFFLSFVKALFTQRRKTTRNAIRNTAHISGLDDPDAVVAAVDDDVLGTR 254
Query: 241 PHS 243
P S
Sbjct: 255 PGS 257
>gb|AAF56847.1| (AE003768) CG11837 gene product [Drosophila melanogaster]
Length = 306
Score = 152 bits (381), Expect = 3e-36
Identities = 89/228 (39%), Positives = 133/228 (58%), Gaps = 10/228 (4%)
Query: 2 RDRLFFLLSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELS 61
+ R+ + K GI N GQH L VI +E A + +DVVLE+GPG G +T +
Sbjct: 9 KSRIHNDVQKQGIVFNKDFGQHILKNPLVITTMLEKAALRATDVVLEIGPGTGNMTVRML 68
Query: 62 KRAKKVFTIELDRRIIEILRNEYSWN----NVEIIQGDAVKVEWPSFNKVVSNIPYQISS 117
+RAKKV E+D R+ L+ ++++ GD +K E P F+ ++N+PYQISS
Sbjct: 69 ERAKKVIACEIDTRLAAELQKRVQATPLQPKLQVLIGDFLKAELPFFDLCIANVPYQISS 128
Query: 118 PFTFKLL--KMEFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGA 175
P FKLL + F AV+M+Q EFA R+ AKPGD+ Y RLS+ TQ LA V+++M++GK
Sbjct: 129 PLIFKLLLHRPLFRCAVLMFQREFAERLVAKPGDKLYCRLSINTQLLARVDMLMKVGKNN 188
Query: 176 FYPKPKVDSALVLITPKKDRIELN----ESLVKALFQHRRKVVSKALR 219
F P PKV+S++V + PK +N + L + F + K ++ +
Sbjct: 189 FRPPPKVESSVVRLEPKNPPPPVNFTEWDGLTRIAFLRKNKTLAATFK 236
>sp|Q09522|YQN1_CAEEL HYPOTHETICAL 34.1 KD PROTEIN E02H1.1 IN CHROMOSOME II
>gi|7498306|pir||T20417 hypothetical protein E02H1.1 -
Caenorhabditis elegans >gi|3875432|emb|CAA87382.1|
(Z47075) contains similarity to Pfam domain: PF00398
(Ribosomal RNA adenine dimethylases), Score=229.3,
E-value=4.4e-71, N=1 [Caenorhabditis elegans]
Length = 304
Score = 150 bits (376), Expect = 1e-35
Identities = 88/215 (40%), Positives = 129/215 (59%), Gaps = 6/215 (2%)
Query: 21 GQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEIL 80
GQH L V++ +E + + +D VLEVGPG G LT ++ + AK V E+D R+I +
Sbjct: 29 GQHILKNPGVVNAIVEKSALKATDTVLEVGPGTGNLTVKMLEVAKTVIACEIDPRMIAEV 88
Query: 81 RNEYSW----NNVEIIQGDAVKVEWPSFNKVVSNIPYQISSPFTFKLL--KMEFERAVVM 134
+ N +++ GD +K+EWP F+ V+N+PYQISSPF KLL + AV+M
Sbjct: 89 KKRVMGTPLQNKLQVNGGDVMKMEWPFFDVCVANLPYQISSPFVQKLLLHRPLPRYAVLM 148
Query: 135 YQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPKKD 194
+Q EFA R+ A+PGD++YSRLS+ Q LA VE++M++ + F P PKVDSA+V I PK
Sbjct: 149 FQKEFADRLVARPGDKDYSRLSVNVQLLAKVEMLMKVKRTEFRPPPKVDSAVVRIAPKNP 208
Query: 195 RIELNESLVKALFQHRRKVVSKALRESAHMLGIKD 229
E L++ F + K + R S + I+D
Sbjct: 209 PPPEWEGLLRLCFMRKNKTLMAIFRLSNVIEVIED 243
>dbj|BAB10912.1| (AB013389) dimethyladenosine transferase-like protein [Arabidopsis
thaliana]
Length = 380
Score = 147 bits (367), Expect = 1e-34
Identities = 79/220 (35%), Positives = 131/220 (58%), Gaps = 10/220 (4%)
Query: 21 GQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEIL 80
GQH L ++D + +++ +D VLE+GPG G LT +L + A+ V +ELD+R++EIL
Sbjct: 68 GQHLLTNTRILDSIVRSSDIRPTDTVLEIGPGTGNLTMKLLEAAQNVVAVELDKRMVEIL 127
Query: 81 RNEYS----WNNVEIIQGDAVKVEWPSFNKVVSNIPYQISSPFTFKLL--KMEFERAVVM 134
R S + + IIQ D +K ++P F+ VV+NIPY ISSP KL+ F A ++
Sbjct: 128 RKRVSDHGFADKLTIIQKDVLKTDFPHFDLVVANIPYNISSPLVAKLVYGSNTFRSATLL 187
Query: 135 YQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPKKD 194
Q EF+ R+ A PGD +++RL++ + +ADV+ VM + K F P PKVDS+++ ITPK+
Sbjct: 188 LQKEFSRRLLANPGDSDFNRLAVNVKLVADVKFVMDVSKREFVPPPKVDSSVIRITPKEI 247
Query: 195 RIELNE----SLVKALFQHRRKVVSKALRESAHMLGIKDV 230
++N + + F + K + R+ ++ ++ +
Sbjct: 248 IPDVNVQEWLAFTRTCFGKKNKTLGSMFRQKKKVMELQSL 287
>gi|11499372 dimethyladenosine transferase (ksgA) [Archaeoglobus fulgidus]
>gi|7433474|pir||F69472 dimethyladenosine transferase
(ksgA) homolog - Archaeoglobus fulgidus
>gi|2648762|gb|AAB89464.1| (AE000979) dimethyladenosine
transferase (ksgA) [Archaeoglobus fulgidus]
Length = 244
Score = 145 bits (361), Expect = 7e-34
Identities = 89/217 (41%), Positives = 128/217 (58%), Gaps = 14/217 (6%)
Query: 20 IGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEI 79
+GQH L+ + VI + + AE+S+ DVVLEVG G G LT L ++ V IE D +++
Sbjct: 7 LGQHMLVDRRVISRIVGYAELSEDDVVLEVGCGTGNLTSALLRKCS-VVGIEKDPLMVKR 65
Query: 80 LRNEYS----WNNVEIIQGDAVKVEWPSFNKVVSNIPYQISSPFTFKLLKMEFERAVVMY 135
LR +S +IQGDA+KV++P F K V+NIPY+ISSP TFKLLK +F AVVMY
Sbjct: 66 LRERFSDFIGKGRFRLIQGDALKVDFPYFTKFVANIPYKISSPLTFKLLKTDFRLAVVMY 125
Query: 136 QLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPKK-- 193
Q EFA R+ + +RL ++++ EI+ + +F P PKV+SA+V I P+
Sbjct: 126 QREFAERLCGED-----NRLGVISKTYCKAEILEIVKPSSFNPPPKVESAIVRIVPEPEV 180
Query: 194 --DRIELNESLVKALFQHRRKVVSKALRESAHMLGIK 228
+ EL E V F RRK + K ++E G++
Sbjct: 181 FVENRELFEKFVTFAFSMRRKRMGKIVQEFRKRYGVE 217
>pir||T40240 dimethylase - fission yeast (Schizosaccharomyces pombe)
>gi|6048291|emb|CAB58154.1| (AL121815) dimethylase
[Schizosaccharomyces pombe]
Length = 307
Score = 140 bits (350), Expect = 1e-32
Identities = 81/216 (37%), Positives = 123/216 (56%), Gaps = 10/216 (4%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRR- 75
N GQH L V ++ A++ +SD VLEVGPG G LT + ++A+KV +E+D R
Sbjct: 25 NKDFGQHILKNPLVAQGIVDKADLKQSDTVLEVGPGTGNLTVRMLEKARKVIAVEMDPRM 84
Query: 76 ---IIEILRNEYSWNNVEIIQGDAVKVEWPSFNKVVSNIPYQISSPFTFKLLKMEF--ER 130
I + ++ ++++ GD +K + P F+ VSN PYQISSP FKLL+
Sbjct: 85 AAEITKRVQGTPKEKKLQVVLGDVIKTDLPYFDVCVSNTPYQISSPLVFKLLQQRPAPRA 144
Query: 131 AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
A++M+Q EFALR+ A+PGD Y RLS Q A V+ +M++GK F P P V+S++V I
Sbjct: 145 AILMFQREFALRLVARPGDPLYCRLSANVQMWAHVKHIMKVGKNNFRPPPLVESSVVRIE 204
Query: 191 PKKD----RIELNESLVKALFQHRRKVVSKALRESA 222
PK E + L++ +F + K + + S+
Sbjct: 205 PKNPPPPLAFEEWDGLLRIVFLRKNKTIGACFKTSS 240
>sp|P37468|KSGA_BACSU DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLMETHIONINE-6-N',
N'-ADENOSYL(RRNA) DIMETHYLTRANSFERASE) (16S RRNA
DIMETHYLASE) (HIGH LEVEL KASUGAMYCIN RESISTANCE PROTEIN
KSGA) (KASUGAMYCIN DIMETHYLTRANSFERASE)
>gi|2126951|pir||S66071 probable
(adenine-N6,N6-)-dimethyltransferase (EC 2.1.1.-) ksgA -
Bacillus subtilis >gi|467431|dbj|BAA05277.1| (D26185)
high level kasgamycin resistance [Bacillus subtilis]
>gi|2632309|emb|CAB11818.1| (Z99104) dimethyladenosine
transferase [Bacillus subtilis]
Length = 292
Score = 139 bits (348), Expect = 2e-32
Identities = 90/258 (34%), Positives = 144/258 (54%), Gaps = 20/258 (7%)
Query: 8 LLSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKV 67
+L KYG +GQ+FLI +++++ ++ AEV++ V+E+GPG+G LT++L+KRAKKV
Sbjct: 14 ILKKYGFSFKKSLGQNFLIDTNILNRIVDHAEVTEKTGVIEIGPGIGALTEQLAKRAKKV 73
Query: 68 FTIELDRRIIEILRNEYS-WNNVEIIQGDAVKVEWPSFNK----------VVSNIPYQIS 116
E+D+R++ IL++ S + NV +I D +K + S + VV+N+PY ++
Sbjct: 74 VAFEIDQRLLPILKDTLSPYENVTVIHQDVLKADVKSVIEEQFQDCDEIMVVANLPYYVT 133
Query: 117 SPFTFKLLK--MEFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKG 174
+P KLL+ + + VVM Q E A RM A P + Y LS+ Q + + VM + K
Sbjct: 134 TPIIMKLLEEHLPLKGIVVMLQKEVAERMAADPSSKEYGSLSIAVQFYTEAKTVMIVPKT 193
Query: 175 AFYPKPKVDSALV-LITPKKDRIEL-NES----LVKALFQHRRKVVSKALRESAHMLGIK 228
F P+P VDSA++ LI +++ NES L+KA F RRK + L + G
Sbjct: 194 VFVPQPNVDSAVIRLILRDGPAVDVENESFFFQLIKASFAQRRKTLLNNLVNNLPE-GKA 252
Query: 229 DVKTVKDILSSVPHSNKR 246
T++ +L KR
Sbjct: 253 QKSTIEQVLEETNIDGKR 270
>sp|P78697|DIM1_KLULA DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLMETHIONINE-6-N',
N'-ADENOSYL(RRNA) DIMETHYLTRANSFERASE) (18S RRNA
DIMETHYLASE)
Length = 320
Score = 139 bits (347), Expect = 3e-32
Identities = 90/259 (34%), Positives = 143/259 (54%), Gaps = 12/259 (4%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N +GQH L V ++ A++ SD+VLE+GPG G LT + ++A+KV +E D R+
Sbjct: 32 NTDLGQHILKNPLVAQGIVDKAQIKPSDIVLEIGPGTGNLTVRILEQARKVVAVEFDPRM 91
Query: 77 IEILRNEYSWNNVE----IIQGDAVKVEWPSFNKVVSNIPYQISSPFTFKLLKMEFER-- 130
+ VE I+ GD +K E P F+ +SN PYQISSP FKL+
Sbjct: 92 AADVTKRVHGTPVEKKLEILLGDFMKTELPYFDVCISNTPYQISSPLVFKLINQPKPPRV 151
Query: 131 AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+++M+Q EFA+R+ A+PGD Y RLS Q A+V +M++GK F P PKV+S++V I
Sbjct: 152 SILMFQREFAMRLLARPGDSLYCRLSANVQMWANVTHIMKVGKNNFRPPPKVESSVVRIE 211
Query: 191 PKKDR--IELNE--SLVKALFQHRRKVVSKALRESAHM-LGIKDVKTVKDILSSVPHSNK 245
K R ++ NE L++ +F + + ++ + + + + K+ K S+VP ++
Sbjct: 212 IKNPRPQVDFNEWDGLLRIVFVRKNRTIAAGFKSTTDLEILEKNYKAFLATQSAVPTTSS 271
Query: 246 RVFHLTPEEVKEIEEYLRE 264
L E ++IE+ L E
Sbjct: 272 G-DSLINEVKEKIEQVLSE 289
>gi|6324989 dimethyladenosine transferase; Dim1p [Saccharomyces cerevisiae]
>gi|1169344|sp|P41819|DIM1_YEAST DIMETHYLADENOSINE
TRANSFERASE (S-ADENOSYLMETHIONINE-6-N',
N'-ADENOSYL(RRNA) DIMETHYLTRANSFERASE) (18S RRNA
DIMETHYLASE) >gi|1078347|pir||S47985 rRNA
(adenine-N6,N6-)-dimethyltransferase (EC 2.1.1.-) DIM1 -
yeast (Saccharomyces cerevisiae)
>gi|601876|gb|AAA57357.1| (L26480) dimethyladenosine
transferase [Saccharomyces cerevisiae]
>gi|1370549|emb|CAA98001.1| (Z73622) ORF YPL266w
[Saccharomyces cerevisiae]
Length = 318
Score = 138 bits (345), Expect = 5e-32
Identities = 92/259 (35%), Positives = 142/259 (54%), Gaps = 13/259 (5%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N +GQH L V ++ A++ SDVVLEVGPG G LT + ++AK V +E+D R+
Sbjct: 31 NTDLGQHILKNPLVAQGIVDKAQIRPSDVVLEVGPGTGNLTVRILEQAKNVVAVEMDPRM 90
Query: 77 IEILRNEYSWNNVE----IIQGDAVKVEWPSFNKVVSNIPYQISSPFTFKLLKMEFER-- 130
L VE I+ GD +K E P F+ +SN PYQISSP FKL+
Sbjct: 91 AAELTKRVRGTPVEKKLEIMLGDFMKTELPYFDICISNTPYQISSPLVFKLINQPRPPRV 150
Query: 131 AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+++M+Q EFALR+ A+PGD Y RLS Q A+V +M++GK F P P+V+S++V +
Sbjct: 151 SILMFQREFALRLLARPGDSLYCRLSANVQMWANVTHIMKVGKNNFRPPPQVESSVVRLE 210
Query: 191 PKKDR--IELNE--SLVKALFQHRRKVVSKALRESAHM-LGIKDVKTVKDILSSVPHSNK 245
K R ++ NE L++ +F + + +S + + M + K+ KT + + + K
Sbjct: 211 IKNPRPQVDYNEWDGLLRIVFVRKNRTISAGFKSTTVMDILEKNYKTFLAMNNEMVDDTK 270
Query: 246 RVFHLTPEEVKEIEEYLRE 264
H +E +I+ L+E
Sbjct: 271 GSMHDVVKE--KIDTVLKE 287
>pir||T43249 rRNA (adenine-N6,N6-)-dimethyltransferase (EC 2.1.1.-) - fission
yeast (Schizosaccharomyces pombe)
>gi|4572637|emb|CAA92585.1| (Z68293) dimethylase
[Schizosaccharomyces pombe]
Length = 315
Score = 138 bits (344), Expect = 7e-32
Identities = 80/216 (37%), Positives = 122/216 (56%), Gaps = 10/216 (4%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRR- 75
N GQH L V ++ A++ +SD VLEVGPG G LT + ++A+KV +E+D R
Sbjct: 25 NKDFGQHILKNPLVAQGIVDKADLKQSDTVLEVGPGTGNLTVRMLEKARKVIAVEMDPRM 84
Query: 76 ---IIEILRNEYSWNNVEIIQGDAVKVEWPSFNKVVSNIPYQISSPFTFKLLKMEF--ER 130
I + ++ ++++ GD +K + P F+ VSN PYQISSP FKLL+
Sbjct: 85 AAEITKRVQGTPKEKKLQVVLGDVIKTDLPYFDVCVSNTPYQISSPLVFKLLQQRPAPRG 144
Query: 131 AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
A++M+Q EFALR+ A+PGD Y RL Q A V+ +M++GK F P P V+S++V I
Sbjct: 145 AILMFQREFALRLVARPGDPLYCRLPANVQMWAHVKHIMKVGKNNFRPPPLVESSVVRIE 204
Query: 191 PKKD----RIELNESLVKALFQHRRKVVSKALRESA 222
PK E + L++ +F + K + + S+
Sbjct: 205 PKNPPPPLAFEEWDGLLRIVFLRKNKTIGACFKTSS 240
>dbj|BAB03776.1| (AP001507) dimethyladenosine transferase [Bacillus halodurans]
Length = 289
Score = 136 bits (340), Expect = 2e-31
Identities = 89/276 (32%), Positives = 155/276 (55%), Gaps = 20/276 (7%)
Query: 8 LLSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKV 67
+L+K+G +GQ+FLI +V+ ++VA ++ +E+GPG+G LT++L+++AK+V
Sbjct: 14 ILAKHGFTFKKSLGQNFLIDPNVLRNIVDVASLTPQSGAIEIGPGIGALTEQLARQAKRV 73
Query: 68 FTIELDRRIIEILRNEYS-WNNVEIIQGDAVKVE-----WPSFNK-----VVSNIPYQIS 116
E+D+R+I +LR + + NV +I D +K + +F + VV+N+PY ++
Sbjct: 74 VAFEIDQRLIPVLRETLAPYENVTVINEDVLKADVKQVIATTFEEGQDLMVVANLPYYVT 133
Query: 117 SPFTFKLL--KMEFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKG 174
+P KLL K+ VVM Q E A R+ AKPG + Y LS+ Q A E+ +++ K
Sbjct: 134 TPILMKLLEAKLPVRGIVVMIQAEVADRIAAKPGTKEYGSLSIAAQYYATAELAVKVPKT 193
Query: 175 AFYPKPKVDSALVLIT-PKKDRIELNE-----SLVKALFQHRRKVVSKALRESAHMLGIK 228
F P+P VDSA++ +T +K + + + ++V+A F +RRK + L + K
Sbjct: 194 VFVPQPNVDSAVLRLTIREKPPVTVADEEWLFAIVRASFANRRKTILNNLIHNLVGKERK 253
Query: 229 DVKTVKDILSSVPHSNKRVFHLTPEEVKEIEEYLRE 264
D K V+ + + ++R LT EE + E L++
Sbjct: 254 D-KAVQALAEAGIDPSRRGETLTIEEFANLSEQLQK 288
>pir||B72255 dimethyladenosine transferase - Thermotoga maritima (strain MSB8)
>gi|4982002|gb|AAD36507.1|AE001795_10 (AE001795)
dimethyladenosine transferase [Thermotoga maritima]
Length = 279
Score = 132 bits (329), Expect = 4e-30
Identities = 84/259 (32%), Positives = 135/259 (51%), Gaps = 17/259 (6%)
Query: 9 LSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVF 68
L KYG+R +GQ FL + + ++ AE++ DVV+E+G G G LT+EL+K +V
Sbjct: 26 LKKYGVRLKKHLGQVFLSDDRIAKRIVKAAELTPEDVVVEIGAGAGTLTEELAKTGARVI 85
Query: 69 TIELDRRIIEILRNEYS-WNNVEIIQGDAVKVE-WPSFNKVVSNIPYQISSPFTFKLLKM 126
E+D + IL+ S + NVE+ D +K + P VSNIPY I+ P K+++
Sbjct: 86 AYEIDESLAPILQERLSKYPNVELRFEDFLKAKNVPEGAICVSNIPYNITGPLMEKIIEW 145
Query: 127 EFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSAL 186
+F+RA+VM Q E R+ AKPG + Y LS++ Q +V+ + + + F P P+VDS +
Sbjct: 146 KFKRAIVMIQKEVGERILAKPGKKTYGYLSVVVQTFYEVKKLFDVSRSCFVPNPEVDSTV 205
Query: 187 VLITPKKDRIELN--ESLVKALFQHRRKVVSKALRESAHMLGIKDVKTVKDILSSVPHSN 244
V + K ++ + V +F +RK + LR + D+ +
Sbjct: 206 VDLKRKPVDLDFEKFKKFVSMIFAKKRKTLKNNLRPFLSIFEGVDL-------------S 252
Query: 245 KRVFHLTPEEVKEIEEYLR 263
+R LT EE+ E+ E R
Sbjct: 253 RRAEQLTVEEIVELYEKWR 271
>pir||T09625 rRNA (adenine-N6,N6-)-dimethyltransferase (EC 2.1.1.-) [validated]
- yeast (Kluyveromyces marxianus var. lactis)
>gi|1806115|emb|CAA92586.1| (Z68294) dimethylase
[Kluyveromyces lactis]
Length = 320
Score = 128 bits (318), Expect = 8e-29
Identities = 90/267 (33%), Positives = 143/267 (52%), Gaps = 28/267 (10%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N +GQH L V ++ A++ SD+VLE+GPG G LT + ++A+KV RR
Sbjct: 32 NTDLGQHILKNPLVAQGIVDKAQIKPSDIVLEIGPGTGNLTVRILEQARKVV-----RRG 86
Query: 77 IEILRNEYSWNN------------VEIIQGDAVKVEWPSFNKVVSNIPYQISSPFTFKLL 124
+ R+ Y +EI+ GD +K E P F+ +SN PYQISSP FKL+
Sbjct: 87 V---RSSYGSGRTKRVHGTPVEKKLEILLGDFMKTELPYFDVCISNTPYQISSPLVFKLI 143
Query: 125 KMEFER--AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKV 182
+++M+Q EFA+R+ A+PGD Y RLS Q A+V +M++GK F P PKV
Sbjct: 144 NQPKPPRVSILMFQREFAMRLLARPGDSLYCRLSANVQMWANVTHIMKVGKNNFRPPPKV 203
Query: 183 DSALVLITPKKDR--IELNE--SLVKALFQHRRKVVSKALRESAHM-LGIKDVKTVKDIL 237
+S++V I K R ++ NE L++ +F + + ++ + + + + K+ K
Sbjct: 204 ESSVVRIEIKNPRPQVDFNEWDGLLRIVFVRKNRTIAAGFKSTTDLEILEKNYKAFLATQ 263
Query: 238 SSVPHSNKRVFHLTPEEVKEIEEYLRE 264
S+VP ++ L E ++IE+ L E
Sbjct: 264 SAVPTTSSG-DSLINEVKEKIEQVLSE 289
>sp|P43038|KSGA_MYCCA DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLMETHIONINE-6-N',
N'-ADENOSYL(RRNA) DIMETHYLTRANSFERASE) (16S RRNA
DIMETHYLASE) (HIGH LEVEL KASUGAMYCIN RESISTANCE PROTEIN
KSGA) (KASUGAMYCIN DIMETHYLTRANSFERASE)
Length = 266
Score = 127 bits (315), Expect = 2e-28
Identities = 81/264 (30%), Positives = 145/264 (54%), Gaps = 26/264 (9%)
Query: 21 GQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEIL 80
GQ+F+ ++I+ ++V + +K +++E+GPG G LT EL KR KV IE+D+ ++EIL
Sbjct: 8 GQNFISDLNLINXIVDVLDQNKDQLIIEIGPGXGALTKELVKRFDKVVVIEIDQDMVEIL 67
Query: 81 RNEYSWNNVEIIQGDAVKVEWPSF--------NKVVSNIPYQISSPFTFKLLKME--FER 130
+ +++ +N+EIIQ D ++++ ++SN PY I+S FK L++ +
Sbjct: 68 KTKFNHSNLEIIQADVLEIDLKQLISKYDYKNISIISNTPYYITSEILFKTLQISDLLTK 127
Query: 131 AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
AV M Q E ALR+ + + NY+ LS+ Q + + K FYP PKVDSA++ +T
Sbjct: 128 AVFMLQKEVALRICSNKNENNYNNLSIACQFYSQRNFEFVVNKKMFYPIPKVDSAIISLT 187
Query: 191 PKK-DRIELNE-----SLVKALFQHRRKVVSKALRESAHMLGIKDVKTVKDILSSVPHSN 244
+ ++N+ V+ LF ++RK + L I++ + L + S+
Sbjct: 188 FNNIYKKQINDDKKFIEFVRTLFNNKRKTILNNLNNI-----IQNKNKALEYLKMLNISS 242
Query: 245 KRVFHLTPEEVKEIEEYLREHRII 268
+L PE++ +I+EY++ +I
Sbjct: 243 ----NLRPEQL-DIDEYIKLFNLI 261
>pir||H81168 dimethyladenosine transferase NMB0697 [imported] - Neisseria
meningitidis (group B strain MD58)
>gi|7225925|gb|AAF41114.1| (AE002424) dimethyladenosine
transferase [Neisseria meningitidis MC58]
Length = 259
Score = 124 bits (309), Expect = 9e-28
Identities = 83/255 (32%), Positives = 130/255 (50%), Gaps = 20/255 (7%)
Query: 19 RIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIE 78
R GQ+FL +I + DVV+E+GPGL +T+ L+K+ ++ +E+DR I+
Sbjct: 9 RFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLAAITEPLAKKLNRLHVVEIDRDIVC 68
Query: 79 ILRNEYSWNNVEIIQGDAVKVEWPSF---NKVVSNIPYQISSPFTFKLLKMEFERAVV-- 133
L+ + + I +GD ++ ++ K+V N+PY IS+P FKL ++ + +
Sbjct: 69 RLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVGNLPYNISTPLLFKLAEVADDVVDMHF 128
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPKK 193
M Q E RM A P +Y RL +M Q D+E+++ + +F P PKVDSA+V + P K
Sbjct: 129 MLQKEVVERMVAAPKSNDYGRLGVMLQYFFDMEMLIDVPPESFDPAPKVDSAVVRMIPVK 188
Query: 194 DRI------ELNESLVKALFQHRRKVVSKALRESAHMLGIKDVKTVKDILSSVPHSNKRV 247
RI E LVK F RRK + L+E A G D++ V + R
Sbjct: 189 HRIGKADDFEHFAKLVKLAFHQRRKTIRNNLKELA---GDDDLQAVG------INPQDRA 239
Query: 248 FHLTPEEVKEIEEYL 262
H+ PE+ + YL
Sbjct: 240 EHIAPEKYVALSNYL 254
>pir||F81936 probable dimethyladenosine transferase (EC 2.1.1.-) NMA0902
[imported] - Neisseria meningitidis (group A strain
Z2491) >gi|7379614|emb|CAB84179.1| (AL162754) putative
dimethyladenosine transferase [Neisseria meningitidis
Z2491]
Length = 259
Score = 124 bits (308), Expect = 1e-27
Identities = 83/255 (32%), Positives = 130/255 (50%), Gaps = 20/255 (7%)
Query: 19 RIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIE 78
R GQ+FL +I + DVV+E+GPGL +T+ L+K+ ++ +E+DR I+
Sbjct: 9 RFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLAAITEPLAKKLNRLHVVEIDRDIVC 68
Query: 79 ILRNEYSWNNVEIIQGDAVKVEWPSF---NKVVSNIPYQISSPFTFKLLKMEFERAVV-- 133
L+ + + I +GD ++ ++ K+V N+PY IS+P FKL ++ + +
Sbjct: 69 RLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVGNLPYNISTPLLFKLAEVADDVVDMHF 128
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPKK 193
M Q E RM A P +Y RL +M Q D+E+++ + +F P PKVDSA+V + P K
Sbjct: 129 MLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDMEMLIDVPPESFDPAPKVDSAVVRMIPVK 188
Query: 194 DRI------ELNESLVKALFQHRRKVVSKALRESAHMLGIKDVKTVKDILSSVPHSNKRV 247
RI E LVK F RRK + L+E A G D++ V + R
Sbjct: 189 HRIGKADDFEHFAKLVKLAFHQRRKTIRNNLKELA---GDDDLQAVG------INPQDRA 239
Query: 248 FHLTPEEVKEIEEYL 262
H+ PE+ + YL
Sbjct: 240 EHIAPEKYVALSNYL 254
>pir||E70173 dimethyladenosine transferase (ksgA) homolog - Lyme disease
spirochete >gi|2688507|gb|AAB91517.1| (AE001160)
dimethyladenosine transferase (ksgA) [Borrelia
burgdorferi]
Length = 281
Score = 120 bits (297), Expect = 2e-26
Identities = 74/219 (33%), Positives = 125/219 (56%), Gaps = 10/219 (4%)
Query: 9 LSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVF 68
L + I P GQ++LI + + K IE ++ +++ + E+GPGLG +T+ L K+ +
Sbjct: 22 LKERKIAPRKLWGQNYLINESIRQKIIESLDIKENEKIWEIGPGLGAMTEILLKKTNLLT 81
Query: 69 TIELDRRIIEILRNEYS-WNNVEIIQGDAVKV---EWPSFNKVVSNIPYQISSPFTFKLL 124
E+D + EIL ++ N ++I+GD +K E + +K+ SN+PY I+S KL+
Sbjct: 82 AFEIDLKYSEILNEKFGKLKNFKLIKGDFLKKYKNENQNIDKIFSNLPYNIASKVISKLI 141
Query: 125 KMEFERAVVM-YQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVD 183
+ F + +V Q E A R+TAK +NYS +++ Q+ V ++ IG+ FYP PKV
Sbjct: 142 EENFLKEMVFTVQKELADRITAKINSKNYSSFTVLVQSHFKVIKILDIGENNFYPAPKVK 201
Query: 184 SALVLITPKKDRI----ELNESLVKALFQHRRKVVSKAL 218
S + + PKK+ I E N+ LV+ +F +RRK + +
Sbjct: 202 STTLKLIPKKNNIKNFKEFNK-LVRTVFSNRRKKLKNTI 239
>pir||H83571 rRNA (adenine-N6,N6)-dimethyltransferase PA0592 [imported] -
Pseudomonas aeruginosa (strain PAO1)
>gi|9946464|gb|AAG03981.1|AE004495_5 (AE004495) rRNA
(adenine-N6,N6)-dimethyltransferase [Pseudomonas
aeruginosa]
Length = 268
Score = 119 bits (295), Expect = 4e-26
Identities = 80/238 (33%), Positives = 124/238 (51%), Gaps = 18/238 (7%)
Query: 12 YGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIE 71
Y R R GQ+FL VI + + + +LE+GPG G LT+ L ++ IE
Sbjct: 5 YQHRARKRFGQNFLHDAGVIHRILRAIHAREGQRLLEIGPGQGALTEGLLGSGARLDVIE 64
Query: 72 LDRRIIEILRNEYSWNN-VEIIQGDAVKVEWPSFN------KVVSNIPYQISSPFTFKLL 124
LD+ +I +L+ ++ + + QGDA+K ++ S +VV N+PY IS+P F LL
Sbjct: 65 LDQDLIPLLKLKFGLESRFSLHQGDALKFDFASLVESGEKLRVVGNLPYNISTPLIFHLL 124
Query: 125 KME--FERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKV 182
+ E M Q E R+ A PG ++ RLS+M Q VE + +G GAF P PKV
Sbjct: 125 EHAPVIEDMHFMLQKEVVERLAATPGGGDWGRLSIMVQYHCRVEHLFNVGPGAFNPPPKV 184
Query: 183 DSALVLITPKKD------RIELNESLVKALFQHRRKVVSKALRESAHMLGIKDVKTVK 234
DSA+V +TP + +L E +V+ F RRK + L+ +L ++D++ +
Sbjct: 185 DSAIVRLTPFAEPPHPARDPKLLERVVREAFNQRRKTLRNTLKP---LLSVEDIEAAE 239
>sp|O05952|KSGA_RICPR DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLMETHIONINE-6-N',
N'-ADENOSYL(RRNA) DIMETHYLTRANSFERASE) (16S RRNA
DIMETHYLASE) (HIGH LEVEL KASUGAMYCIN RESISTANCE PROTEIN
KSGA) (KASUGAMYCIN DIMETHYLTRANSFERASE)
>gi|7433483|pir||C71673 dimethyladenosine transferase
(ksgA) RP672 - Rickettsia prowazekii
>gi|2073482|emb|CAA72482.1| (Y11786) dimethyladenosine
transferase [Rickettsia prowazekii]
>gi|3861209|emb|CAA15109.1| (AJ235272) DIMETHYLADENOSINE
TRANSFERASE (ksgA) [Rickettsia prowazekii]
Length = 268
Score = 118 bits (294), Expect = 5e-26
Identities = 86/264 (32%), Positives = 136/264 (50%), Gaps = 24/264 (9%)
Query: 10 SKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDE-LSKRAKKVF 68
+ + I P + GQ+F+ + DK I + V ++ V+E+GPG+G LT L K K +
Sbjct: 10 TSHKINPLKKHGQNFIFDSSLCDKIIRASNVLENSNVIEIGPGIGGLTRSILQKNPKSLT 69
Query: 69 TIELDRRIIEILRN--EYSWNNVEIIQGDAVKVEWPS--FNKV--VSNIPYQISSPFTFK 122
IE+D R I +L EY + N+ II+ D +K+ ++KV +SN+PY I + +
Sbjct: 70 VIEIDERCIPLLNEIQEY-YPNLNIIKQDVLKINLTDLIYDKVTVISNLPYHIGTELVIR 128
Query: 123 LLKME--FERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKP 180
LLK +M Q E R+ A P + Y RLS++ Q LA VE + AFYP P
Sbjct: 129 LLKEVKLITNMTLMLQKEVVERICAIPSTKAYGRLSVICQILAKVEKCFNVAPTAFYPHP 188
Query: 181 KVDSALVLITPKKD-----RIELNESLVKALFQHRRKVVSKALRESAHMLGIKDVKTVKD 235
KV SA+V I P ++ I E + K +F RRK++ +LR + + +
Sbjct: 189 KVYSAIVKIIPLENPPSIALINKVEQITKLVFAGRRKMIKSSLRNL--------IPNIHE 240
Query: 236 ILSSVP-HSNKRVFHLTPEEVKEI 258
+L+ + + N R +LTP++ I
Sbjct: 241 VLTQLKINCNDRAENLTPKDYLRI 264
>pir||F75385 dimethyladenosine transferase - Deinococcus radiodurans (strain
R1) >gi|6459288|gb|AAF11091.1|AE001996_6 (AE001996)
dimethyladenosine transferase [Deinococcus radiodurans]
Length = 336
Score = 118 bits (294), Expect = 5e-26
Identities = 72/227 (31%), Positives = 122/227 (53%), Gaps = 7/227 (3%)
Query: 4 RLFFLLSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKR 63
R+ LL+ +G++P +GQ+FLI +++ E + + VLE+GPGLG LT E++ R
Sbjct: 71 RVRALLAAHGLKPTKSLGQNFLIDGNILRAIAEAGGAAPGENVLEIGPGLGVLTREVASR 130
Query: 64 AKKVFTIELDRRIIEILRNEYSWNNVEIIQGDAVKVEW---PSFNKVVSNIPYQISSPFT 120
+V +E D R+ +L + +V +I GDA+ ++ P+ +V++N+PY I+
Sbjct: 131 GARVTALEKDERLRPVLAETLAGLDVNVIWGDALDFDYAALPAGTRVIANLPYYITGLLL 190
Query: 121 FKLLKME-FERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPK 179
+ ++ A V+ Q E A R+ A+PG NY LS + V+ V + KGAF+P
Sbjct: 191 TRFMQAPGVVSATVLVQKEVAQRLVAQPGQDNYGFLSAVAALYGSVKHVRDVPKGAFFPA 250
Query: 180 PKVDSALVLITPKKDRIELNE---SLVKALFQHRRKVVSKALRESAH 223
P V S++V + + R + + S V ++RRK + LR H
Sbjct: 251 PDVTSSVVRLDFDRTRPQPDPAFVSFVDNALRYRRKTLRNNLRMMGH 297
>pir||D81680 dimethyladenosine transferase TC0633 [imported] - Chlamydia
muridarum (strain Nigg) >gi|7190672|gb|AAF39462.1|
(AE002332) dimethyladenosine transferase [Chlamydia
muridarum]
Length = 277
Score = 115 bits (284), Expect = 8e-25
Identities = 89/279 (31%), Positives = 139/279 (48%), Gaps = 33/279 (11%)
Query: 7 FLLSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKK 66
FL S G R + Q+FL+ +++ K + A+V D VLE+GPG G L++ L +
Sbjct: 12 FLKSVNG-RAKKALSQNFLVDGNILRKILATADVQPGDWVLEIGPGFGALSEVLVSQGAN 70
Query: 67 VFTIELDRRIIEILRNEYSWNNVEIIQGDAVKVE--------WPSFNKVVSNIPYQISSP 118
V +E D E L S ++I DA K W ++V+N+PY I++P
Sbjct: 71 VIALEKDPMFEESL----SQLPIDIEITDACKYPLASLDDKGWKGKGRIVANLPYHITTP 126
Query: 119 FTFKLL---KMEFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGA 175
K ++ VM Q E A R+TA PGD++YS L++ + ADV+ ++
Sbjct: 127 LLTKFFLECPNRWKTVTVMIQDEVARRITANPGDKDYSSLTVFLRFFADVQYAFKVSPNC 186
Query: 176 FYPKPKVDSALVLITPKKD----RIELNE--SLVKALFQHRRKVVSKALRESAHMLGIKD 229
FYPKP V SA+V + +D E++E +L +A F RRK+++ +L+ L KD
Sbjct: 187 FYPKPSVSSAVVHMRVHEDFPLSGSEIDEFFALTRAAFGQRRKLLANSLKN----LYPKD 242
Query: 230 VKTVKDILSSVPHSNKRVFHLTPEEVKEIEEYLREHRII 268
V +L + S K T E +EEYL+ R++
Sbjct: 243 --KVFQVLEHLGFSEK-----TRPETISLEEYLKIFRLL 274
>pir||H72001 dimethyladenosine transferase CP0791 [imported] - Chlamydophila
pneumoniae (strains CWL029 and AR39)
>gi|4377394|gb|AAD19196.1| (AE001686) Dimethyladenosine
Transferase [Chlamydophila pneumoniae CWL029]
>gi|7189706|gb|AAF38590.1| (AE002238) dimethyladenosine
transferase [Chlamydophila pneumoniae AR39]
>gi|8979432|dbj|BAA99266.1| (AP002548) dimethyladenosine
transferase [Chlamydophila pneumoniae J138]
Length = 277
Score = 114 bits (282), Expect = 1e-24
Identities = 79/228 (34%), Positives = 122/228 (52%), Gaps = 21/228 (9%)
Query: 9 LSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVF 68
LS+ +P + Q+FL+ ++++ K + +EV D VLE+GPG G LT+EL +V
Sbjct: 13 LSEIQNKPKKSLSQNFLVDQNIVKKIVATSEVIPQDWVLEIGPGFGALTEELIAAGAQVI 72
Query: 69 TIELDRRIIEILRNEYSWNNVEIIQGDAVKV------EWPSFNK--VVSNIPYQISSPFT 120
IE D L E +EII DA K E+ + K VV+N+PY I++P
Sbjct: 73 AIEKDPMFAPSL--EELPIRLEII--DACKYPLDQLQEYKTLGKGRVVANLPYHITTPLL 128
Query: 121 FKLL--KMEFERAV-VMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFY 177
KL +F + V VM Q E A R+ A+PG R+Y L++ Q AD+ ++ FY
Sbjct: 129 TKLFLEAPDFWKTVTVMVQDEVARRIVAQPGGRDYGSLTIFLQFFADIHYAFKVSASCFY 188
Query: 178 PKPKVDSALVLITPKK------DRIELNESLVKALFQHRRKVVSKALR 219
PKP+V SA++ + K+ + I + +L + FQ RRKV++ L+
Sbjct: 189 PKPQVQSAVIHMKVKETLPLSDEEIPVFFTLTRTAFQQRRKVLANTLK 236
>pir||B82323 dimethyladenosine transferase VC0443 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9654867|gb|AAF93616.1|
(AE004131) dimethyladenosine transferase [Vibrio
cholerae]
Length = 282
Score = 112 bits (278), Expect = 4e-24
Identities = 79/237 (33%), Positives = 116/237 (48%), Gaps = 24/237 (10%)
Query: 13 GIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIEL 72
G + R GQ+FL +ID + ++E+GPGLG +T+ + + K IEL
Sbjct: 19 GHKARKRFGQNFLNDPYIIDGIVSAINPKPGQNLVEIGPGLGAITEPVGREVDKFTVIEL 78
Query: 73 DRRIIEILRNEYSW-NNVEIIQGDAVKVEWPSFNK------VVSNIPYQISSPFTFKLLK 125
DR + E LRN + + I +GDA++ ++ K V N+PY IS+P F L
Sbjct: 79 DRDLAERLRNHPELASKLTIHEGDAMRFDFKQLVKPNNKLRVFGNLPYNISTPLMFHLF- 137
Query: 126 MEFERAV----VMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPK 181
EF R + M Q E R+ A PG + Y RL++M Q V V+ + AF P PK
Sbjct: 138 -EFHRDIQDMHFMLQKEVVNRLAAGPGTKAYGRLTVMAQYYCKVVPVLEVPPSAFVPPPK 196
Query: 182 VDSALVLITPKKD------RIELNESLVKALFQHRRKVVSKALR-----ESAHMLGI 227
VDSA+V + P +D +E + +V+ F RRK V + E+ LGI
Sbjct: 197 VDSAVVRLVPYEDLPHPATSLEWLDRVVREGFNQRRKTVRNCYKGLAEPETLETLGI 253
>pir||C71526 probable dimethyladenosine transferase - Chlamydia trachomatis
(serotype D, strain UW3/Cx) >gi|3328775|gb|AAC67949.1|
(AE001308) Dimethyladenosine Transferase [Chlamydia
trachomatis]
Length = 277
Score = 111 bits (274), Expect = 1e-23
Identities = 88/274 (32%), Positives = 132/274 (48%), Gaps = 33/274 (12%)
Query: 7 FLLSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKK 66
FL S G R + Q+FL+ +++ K + AEV D VLE+GPG G L++ L +
Sbjct: 12 FLRSVNG-RAKKALSQNFLVDGNILRKILTTAEVQPGDWVLEIGPGFGALSEVLLSQGAN 70
Query: 67 VFTIELDRRIIEILRNEYSWNNVEIIQGDAVKVE--------WPSFNKVVSNIPYQISSP 118
V +E D E L S ++I DA K W ++V+N+PY I++P
Sbjct: 71 VIALEKDPMFEESL----SQLPMDIEITDACKYPLTSLEDKGWKGKGRIVANLPYHITTP 126
Query: 119 FTFKLL---KMEFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGA 175
K ++ VM Q E A R+TAKPGD++Y L++ ADV+ ++
Sbjct: 127 LLTKFFLECPYRWKTVTVMIQDEVARRITAKPGDKDYGSLTVFLSFFADVQYAFKVSPNC 186
Query: 176 FYPKPKVDSALVLITPKK------DRIELNESLVKALFQHRRKVVSKALRESAHMLGIKD 229
FYPKP V SA+V + + IE +L +A F RRK+++ +L+ L KD
Sbjct: 187 FYPKPSVHSAVVHMRVHEQFALADSEIEEFFTLTRAAFGQRRKLLANSLKN----LYPKD 242
Query: 230 VKTVKDILSSVPHSNKRVFHLTPEEVKEIEEYLR 263
V +L + S K T E +EEYL+
Sbjct: 243 --KVFQVLEQLGFSEK-----TRPETIFLEEYLK 269
>pir||E82871 dimethyladenosine transferase UU601 [imported] - Ureaplasma
urealyticum >gi|6899612|gb|AAF31015.1|AE002158_13
(AE002158) dimethyladenosine transferase [Ureaplasma
urealyticum]
Length = 257
Score = 111 bits (274), Expect = 1e-23
Identities = 73/258 (28%), Positives = 134/258 (51%), Gaps = 18/258 (6%)
Query: 20 IGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEI 79
+GQ+FL+ ++ +K ++VA ++K D++LE+GPG G +T+ L ++ + IELD+R+
Sbjct: 1 MGQNFLLSNNIKNKIVDVANINKDDLILEIGPGWGAITEILVQKTNILIAIELDKRLYAH 60
Query: 80 LRNEYSWNNVEIIQGDAVKVEWPS----FN--------KVVSNIPYQISSPFTFKLLKME 127
L+ +N II D + V+ + +N KVV+N+PY ISS K+++ +
Sbjct: 61 LKTYIKTSNFHIINNDVLCVDLDNLILDYNNTQKIQKIKVVANLPYAISSKIVLKIIQSK 120
Query: 128 -FERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSAL 186
A +M Q E A R+ AK R Y+ +++ Q +I+ + F+P+PKV SA+
Sbjct: 121 LINDAYIMVQKEMAERIGAKVNTRGYNAFTVLVQLFCKTKILFEVNAKEFHPQPKVQSAV 180
Query: 187 VLITPKKDRIELN-ESLVKALFQHRRKVVSKALRESAHMLGIKDVKTVKDILSSVP-HSN 244
+ + + + N E L K L R ++K + ++ I D+K + + N
Sbjct: 181 IHLENLHNSVNFNIEELGKFL---RICFLNKRKKLKNNLSNIYDIKIINQMFIDYNLDMN 237
Query: 245 KRVFHLTPEEVKEIEEYL 262
R ++ P+ ++ YL
Sbjct: 238 LRAENIEPKMFLKLFNYL 255
>pir||S74516 (adenine-N6,N6-)-dimethyltransferase (EC 2.1.1.-) ksgA -
Synechocystis sp. (strain PCC 6803)
>gi|1651740|dbj|BAA16668.1| (D90899) dimethyladenosine
transferase [Synechocystis sp.]
Length = 284
Score = 110 bits (273), Expect = 2e-23
Identities = 82/237 (34%), Positives = 122/237 (50%), Gaps = 33/237 (13%)
Query: 15 RPNDRIGQHFLIVKDVIDKAIEVAEV--------SKSDVVLEVGPGLGFLTDELSKRAKK 66
RP R GQH+L + + + A++ S D +LE+GPG+G LT +L
Sbjct: 4 RPRKRFGQHWLNHEPTLQAIVAAADIQSGAPQSGSLRDRLLEIGPGMGVLTKQLLATGNP 63
Query: 67 VFTIELDRRIIEILRNEYSWN-NVEIIQGD-------AVKVEWPSF---NKVVSNIPYQI 115
V +ELDR + LR + N +++GD A+ ++P F NKVV+NIPY I
Sbjct: 64 VVAVELDRDLCLKLRKKLGQRENFLLLEGDVLILDLNALLQDFPQFSPLNKVVANIPYNI 123
Query: 116 SSPFTFKLLKM-------EFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIV 168
+SP LL FE V++ Q E A R+TA+P + Y LS+ Q LA V+ +
Sbjct: 124 TSPILELLLGTIQKPRVPGFETIVLLVQKEIAERLTAQPSTKAYGALSVRMQYLARVDWI 183
Query: 169 MRIGKGAFYPKPKVDSALVLITP------KKDRIELNESLVKALFQHRRKVVSKALR 219
+ + AF P PKVDSA++ +TP DR L + L+ F +RRK++ L+
Sbjct: 184 VDVPPKAFTPPPKVDSAVIRLTPYPVEQLPGDR-RLLDQLLCLGFANRRKMLRNNLK 239
>emb|CAB95942.1| (AL359989) putative dimethyladenosine transferase [Streptomyces
coelicolor A3(2)]
Length = 286
Score = 109 bits (269), Expect = 4e-23
Identities = 74/228 (32%), Positives = 114/228 (49%), Gaps = 17/228 (7%)
Query: 8 LLSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKV 67
L + G+RP + GQ+F+I + + + + AEV DVV+EVGPGLG LT L + A +V
Sbjct: 18 LAAALGVRPTKQRGQNFVIDANTVRRIVRTAEVRPDDVVVEVGPGLGSLTLALLEAADRV 77
Query: 68 FTIELDRRIIEIL------RNEYSWNNVEIIQGDAVKVE---WPSFNKVVSNIPYQISSP 118
+E+D + L R + ++ DA+ V P+ +V+N+PY ++ P
Sbjct: 78 TAVEIDDVLAGALPATVAARMPARADRFALVHSDAMHVRELPGPAPTALVANLPYNVAVP 137
Query: 119 FTFKLLKM--EFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAF 176
+L ER +VM Q E A R+ A PG + Y S+ A+V+ IG+ F
Sbjct: 138 VLLHMLDTFPGIERTLVMVQAEVADRLAAGPGSKVYGVPSVKANWYAEVKRAGSIGRNVF 197
Query: 177 YPKPKVDSALVLITPKKDRIELNES------LVKALFQHRRKVVSKAL 218
+P P VDS LV + + + + S +V A F RRK + AL
Sbjct: 198 WPAPNVDSGLVSLVRRSEPLRTTASKAEVFAVVDAAFAQRRKTLRAAL 245
>sp|P47701|KSGA_MYCGE DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLMETHIONINE-6-N',
N'-ADENOSYL(RRNA) DIMETHYLTRANSFERASE) (16S RRNA
DIMETHYLASE) (HIGH LEVEL KASUGAMYCIN RESISTANCE PROTEIN
KSGA) (KASUGAMYCIN DIMETHYLTRANSFERASE)
>gi|1361543|pir||B64251 probable methyltransferase (EC
2.1.1.-) ksgA homolog - Mycoplasma genitalium
>gi|3845058|gb|AAC72483.1| (U39728) dimethyladenosine
transferase [Mycoplasma genitalium]
Length = 259
Score = 107 bits (265), Expect = 1e-22
Identities = 76/245 (31%), Positives = 124/245 (50%), Gaps = 10/245 (4%)
Query: 16 PNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRR 75
P+ ++GQ+F + VI + + ++E+G G G LT+ L K IE+D+R
Sbjct: 6 PSRKLGQNFTVNLSVIKRIFAFVKNLNPQAIVEIGVGKGALTNYLLKLKIPYKGIEIDKR 65
Query: 76 IIEILRNEYSWNNVEIIQGDAVKVEWPSFNKVVS-----NIPYQISSPFTFKLLKMEFER 130
+IE L E ++++GD +K ++ SF + +S NIPY I+SP K L+ +
Sbjct: 66 LIEYLLVEKILTEDQLVKGDILKKDFNSFFENLSPLLCGNIPYSITSPIINKFLESKLRS 125
Query: 131 AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
V+M Q EFA R+ AK +YS Q + V +I + AF PKPKVDS L+L+
Sbjct: 126 FVLMTQKEFANRLLAKVNSSDYSAFGAFCQYYLTITTVFKIDRHAFKPKPKVDSTLILLE 185
Query: 191 PKKD-RIELNESL-VKALFQHRRKVVSKALRE---SAHMLGIKDVKTVKDILSSVPHSNK 245
K + L +K F RRK++ L+ ++L I + +K + + S
Sbjct: 186 KNKSVSYDFKFGLFLKQCFNQRRKMLINNLKHFFAVDYLLNIIQKQNLKTSIRAQELSPC 245
Query: 246 RVFHL 250
++F+L
Sbjct: 246 QLFNL 250
>sp|P75113|KSGA_MYCPN DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLMETHIONINE-6-N',
N'-ADENOSYL(RRNA) DIMETHYLTRANSFERASE) (16S RRNA
DIMETHYLASE) (HIGH LEVEL KASUGAMYCIN RESISTANCE PROTEIN
KSGA) (KASUGAMYCIN DIMETHYLTRANSFERASE)
>gi|2146646|pir||S73489 probable
S-adenosylmethionine-6-N',N'-adenosyl(rRNA)
dimethyltransferase (EC 2.1.1.-) ksgA - Mycoplasma
pneumoniae (ATCC 28342) >gi|1673824|gb|AAB95811.1|
(AE000017) S-adenosylmethionine-6-N',N'-adenosyl(rRNA)
dimethyltransferase [Mycoplasma pneumoniae]
Length = 263
Score = 107 bits (264), Expect = 2e-22
Identities = 77/258 (29%), Positives = 124/258 (47%), Gaps = 14/258 (5%)
Query: 16 PNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRR 75
P+ ++GQ+F + + VI K + + ++EVGPG G LT L K IELD+R
Sbjct: 6 PSRKLGQNFTVDQSVIAKTCRLIKSLNPTALIEVGPGKGALTKALLKLQLPYHGIELDKR 65
Query: 76 IIEILRNEYSWNNVEIIQGDAVKVEWPSFNK-----VVSNIPYQISSPFTFKLLKMEFER 130
+ E L ++ GDA+K + + NIPY ISSP L + ++
Sbjct: 66 LAEYLLVNEILTEEQLTIGDALKQNLDQYFPDTIPLLCGNIPYSISSPLIANFLASKLQQ 125
Query: 131 AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
V++ Q EF R+ A NYS + Q ++ V +I K AF PKP+VDS L+L+
Sbjct: 126 FVLVCQWEFGQRLVAPVNSPNYSAFGVFCQYHLQIKSVFKIDKVAFKPKPQVDSVLMLL- 184
Query: 191 PKKDRIELNE---SLVKALFQHRRKVVSKALRESAHMLGIKDVKTVKDILSSVPHSNKRV 247
KK ++ +K F RRK++ L++ + +V +D+ ++V R
Sbjct: 185 KKKPQVAYEAHFGRFLKQCFHQRRKLLVNNLKQLLPPTLLTNVLQQQDLAATV-----RA 239
Query: 248 FHLTPEEVKEIEEYLREH 265
LTP ++ + L+ H
Sbjct: 240 QELTPTQLFRLYLSLKPH 257
>sp|P57241|KSGA_BUCAI DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLMETHIONINE-6-N',
N'-ADENOSYL(RRNA) DIMETHYLTRANSFERASE) (16S RRNA
DIMETHYLASE) (HIGH LEVEL KASUGAMYCIN RESISTANCE PROTEIN
KSGA) (KASUGAMYCIN DIMETHYLTRANSFERASE)
>gi|10038824|dbj|BAB12859.1| (AP001118)
dimethyladenosine transferase [Buchnera sp. APS]
Length = 273
Score = 106 bits (263), Expect = 2e-22
Identities = 70/263 (26%), Positives = 141/263 (52%), Gaps = 26/263 (9%)
Query: 16 PNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRR 75
P + GQ+FLI KD I I++ + ++ ++E+GPGL LT + + +++ IE+D+
Sbjct: 10 PLKKYGQNFLINKDTIKNIIKIIDPKTTETLVEIGPGLAALTKPMCELLEELIVIEIDQD 69
Query: 76 IIEILRNEYSWNNVEIIQGDAVKVEWPS-FNK------VVSNIPYQISSP---FTFKLLK 125
++ +L+ ++ + + +A+ + + F+K + N+PY IS+ F FK +K
Sbjct: 70 LLFLLKKRSFYSKLIVFYQNALNFNFLNLFHKKKQLIRIFGNLPYNISTSLIIFLFKQIK 129
Query: 126 MEFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSA 185
+ + M Q E A R+ + PG+++Y RLS+++Q D++I++ + F P PKV S
Sbjct: 130 V-IQDMNFMLQKEVAERLISTPGNKSYGRLSIISQYYCDIKILLNVSPEDFRPIPKVHSV 188
Query: 186 LVLITPKKD------RIELNESLVKALFQHRRKVVSKALRESAHMLGIKDVKTVKDILSS 239
+ + P + + + ++ K FQ+RRK+ LR S +K++ + K+++
Sbjct: 189 FINLKPHTNSPYFVYNVNILSAITKDAFQNRRKI----LRHS-----LKNLFSEKELIKL 239
Query: 240 VPHSNKRVFHLTPEEVKEIEEYL 262
+SN R +++ ++ YL
Sbjct: 240 EINSNLRAENVSVSHYCKLANYL 262
>pir||A47697 probable methyltransferase (EC 2.1.1.-) - Bacillus anthracis
Length = 287
Score = 104 bits (258), Expect = 9e-22
Identities = 74/207 (35%), Positives = 108/207 (51%), Gaps = 18/207 (8%)
Query: 21 GQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEIL 80
GQH + K +I++ ++ A +S D VLE+G G G LT LS++A KV +E D + ++IL
Sbjct: 23 GQHLMHNKKLIEEIVDRANISIDDTVLELGAGAGALTTVLSQKAGKVLAVENDSKFVDIL 82
Query: 81 -RNEYSWNNVEIIQGDAVKVEWPSFN-KVVSNIPYQISSPFTFKLLK---MEFERAVVMY 135
R +N +II D +K+ P VVSNIPY I++P LL F++ +++
Sbjct: 83 TRKTAQHSNTKIIHQDIMKIHLPKEKFVVVSNIPYAITTPIMKMLLNNPASGFQKGIIVM 142
Query: 136 QLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPKKDR 195
+ A R T+K +Y L + D+ IV I K F P PKVDSA+V IT KKD
Sbjct: 143 EKGAAKRFTSKFIKNSY---VLAWRMWFDIGIVREISKEHFSPPPKVDSAMVRITRKKD- 198
Query: 196 IELNESLVKALFQHRRKVVSKALRESA 222
A H+ + + L E A
Sbjct: 199 ---------APLSHKHYIAFRGLAEYA 216
>sp|Q04720|ERMJ_BACAN RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERYTHROMYCIN RESISTANCE PROTEIN)
>gi|143198|gb|AAA22597.1| (L08389) rRNA
methyltransferase [Bacillus anthracis]
Length = 287
Score = 104 bits (257), Expect = 1e-21
Identities = 74/207 (35%), Positives = 108/207 (51%), Gaps = 18/207 (8%)
Query: 21 GQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEIL 80
GQH + K +I++ ++ A +S D VLE+G G G LT LS++A KV +E D + ++IL
Sbjct: 23 GQHLMHNKKLIEEIVDRANISIDDTVLELGAGKGALTTVLSQKAGKVLAVENDSKFVDIL 82
Query: 81 -RNEYSWNNVEIIQGDAVKVEWPSFN-KVVSNIPYQISSPFTFKLLK---MEFERAVVMY 135
R +N +II D +K+ P VVSNIPY I++P LL F++ +++
Sbjct: 83 TRKTAQHSNTKIIHQDIMKIHLPKEKFVVVSNIPYAITTPIMKMLLNNPASGFQKGIIVM 142
Query: 136 QLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPKKDR 195
+ A R T+K +Y L + D+ IV I K F P PKVDSA+V IT KKD
Sbjct: 143 EKGAAKRFTSKFIKNSY---VLAWRMWFDIGIVREISKEHFSPPPKVDSAMVRITRKKD- 198
Query: 196 IELNESLVKALFQHRRKVVSKALRESA 222
A H+ + + L E A
Sbjct: 199 ---------APLSHKHYIAFRGLAEYA 216
>sp|Q03986|ERMD_BACLI RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERYTHROMYCIN RESISTANCE PROTEIN)
>gi|2126802|pir||I39885 rRNA methylase ermD [similarity]
- Bacillus licheniformis >gi|143201|gb|AAA22599.1|
(M29832) macrolide-lincosamide-streptogramin B
resistance methylase [Bacillus licheniformis]
Length = 287
Score = 104 bits (257), Expect = 1e-21
Identities = 74/207 (35%), Positives = 108/207 (51%), Gaps = 18/207 (8%)
Query: 21 GQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEIL 80
GQH + K +I++ ++ A +S D VLE+G G G LT LS++A KV +E D + ++IL
Sbjct: 23 GQHLMHNKKLIEEIVDRANISIDDTVLELGAGKGALTTVLSQKAGKVLAVENDSKFVDIL 82
Query: 81 -RNEYSWNNVEIIQGDAVKVEWPSFN-KVVSNIPYQISSPFTFKLLK---MEFERAVVMY 135
R +N +II D +K+ P VVSNIPY I++P LL F++ +++
Sbjct: 83 TRKTAQHSNTKIIHQDIMKIHLPKEKFVVVSNIPYAITTPIMKMLLNNPASGFQKGIIVM 142
Query: 136 QLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPKKDR 195
+ A R T+K +Y L + D+ IV I K F P PKVDSA+V IT KKD
Sbjct: 143 EKGAAKRFTSKFIKNSY---VLAWRMWFDIGIVREISKEHFSPPPKVDSAMVRITRKKD- 198
Query: 196 IELNESLVKALFQHRRKVVSKALRESA 222
A H+ + + L E A
Sbjct: 199 ---------APLSHKHYIAFRGLAEYA 216
>dbj|BAA83962.1| (AB024564) ERMK [Bacillus halodurans] >gi|10172993|dbj|BAB04099.1|
(AP001508) rRNA adenine N-6-methyltransferase [Bacillus
halodurans]
Length = 284
Score = 104 bits (256), Expect = 1e-21
Identities = 71/186 (38%), Positives = 104/186 (55%), Gaps = 8/186 (4%)
Query: 16 PNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRR 75
P + GQH + K ++ + ++ A+VSK D VLE+G G G LT LS+RAK+V +E D+
Sbjct: 18 PPNFSGQHLMHNKKLLQEIVDQAKVSKKDTVLELGAGKGALTTFLSERAKRVLAVEYDQT 77
Query: 76 IIEILRNEYSW-NNVEIIQGDAVKVEWPSFN-KVVSNIPYQISSPFTFKLLK---MEFER 130
I++L + + N II D +++ P VVSNIPY I++P KLL F+R
Sbjct: 78 FIQVLNRKMAHAANTTIIHEDIMRIHLPKGEFVVVSNIPYSITTPIMKKLLSNPVSGFQR 137
Query: 131 AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
V++ + A R T+ P +N L + D+E V I + F P PKVDSA+V I+
Sbjct: 138 GVIVMEKGAAKRFTS-PFIKN--AYVLAWRMWFDLEYVKGISRECFSPPPKVDSAMVFIS 194
Query: 191 PKKDRI 196
K D I
Sbjct: 195 RKPDPI 200
>emb|CAB64788.1| (AJ242786) KsgA, high level kasugamycin resistance protein
[Burkholderia sp.]
Length = 277
Score = 104 bits (256), Expect = 1e-21
Identities = 77/219 (35%), Positives = 113/219 (51%), Gaps = 21/219 (9%)
Query: 19 RIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKR---AKKVFTIELDRR 75
R GQHFLI VID + + D ++E+GPGLG LT L +R A ++ IE+DR
Sbjct: 16 RFGQHFLIDSAVIDAIVSAIAPAPQDCLVEIGPGLGALTWPLLERLGGAGRLHAIEMDRD 75
Query: 76 IIEILRNEYSWNNVEIIQGDAVKVEWPSFNK-------VVSNIPYQISSPFTFKLLKMEF 128
+ L+ ++ + GDA++ ++ + +V N+PY ISSP F LL E
Sbjct: 76 LAAHLQR-LGHASLILHAGDALRFDFAQLARAEQARLRIVGNLPYNISSPLLFHLLD-EM 133
Query: 129 ERAV---VMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSA 185
V M Q E A R+ A PG +Y RLS+M Q+ VE + + AF P P V SA
Sbjct: 134 NEVVDQHFMLQREVAARIAAAPGSAHYGRLSVMLQSRYAVERLFDVPPCAFAPPPAVHSA 193
Query: 186 LVLITPKKDRI--ELN----ESLVKALFQHRRKVVSKAL 218
++ + P R +L+ +LV+A F RRK++ L
Sbjct: 194 VLRMAPHASRALPQLDWARFAALVRAAFSQRRKILRHTL 232
>pir||A72640 probable dimethyladenosine transferase APE0553 - Aeropyrum pernix
(strain K1) >gi|5104206|dbj|BAA79521.1| (AP000060) 277aa
long hypothetical dimethyladenosine transferase
[Aeropyrum pernix]
Length = 277
Score = 103 bits (255), Expect = 2e-21
Identities = 79/264 (29%), Positives = 128/264 (47%), Gaps = 15/264 (5%)
Query: 8 LLSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVV---LEVGPGLGFLTDELSKRA 64
+L G+RP+DR+GQHFLI + + ++ E + ++ + LE+GPG G +T ++
Sbjct: 20 VLGLAGLRPSDRLGQHFLIDDRAVGEFLKPLEKAAAEGIREALEIGPGAGSITLPAAEVL 79
Query: 65 KKVFTIELDRRIIEILRNEYSWNNVEIIQGDAVKVEWPSFNKVV-SNIPYQISSPFTFKL 123
++ +ELD R+ L + + V +I GD V S +V SN P+ +S L
Sbjct: 80 DRIVAVELDNRLASAL-SRLAPARVAVITGDGVSHAAASQAPLVFSNTPFNLSPAIVEAL 138
Query: 124 -LKMEFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKV 182
+ AV+ Q E A RMTA+PG R+YSRLS++ + E+ + A+YP+P+V
Sbjct: 139 AVNNRVAAAVLGVQYEVARRMTARPGSRDYSRLSVLVSLVFHAELAGVVRPQAYYPRPQV 198
Query: 183 DSALVLITPKKDRIELNE---SLVKALFQHRRKVVSKALRESAHMLGIKDVKTVKDILSS 239
+A+V + ++ L L F R K SK LR G + +
Sbjct: 199 LTAVVTLRRRRRWRSLYARALELAGCAFTQRNKKASKVLRRCLEAAGCAPPPWLDSL--- 255
Query: 240 VPHSNKRVFHLTPEEVKEIEEYLR 263
+ RV+ L PE+ + E R
Sbjct: 256 ---GDARVWMLRPEDFVGLAEACR 276
>pir||H82593 dimethyladenosine transferase XF2148 [imported] - Xylella
fastidiosa (strain 9a5c)
>gi|9107283|gb|AAF84947.1|AE004029_7 (AE004029)
dimethyladenosine transferase [Xylella fastidiosa]
Length = 290
Score = 102 bits (252), Expect = 4e-21
Identities = 73/219 (33%), Positives = 112/219 (50%), Gaps = 22/219 (10%)
Query: 21 GQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEIL 80
GQHFL+ + ID+ I +D ++E+GPG G +T L K + IELDR +I L
Sbjct: 40 GQHFLVDRYYIDRIIHAITPQPNDHIVEIGPGQGAITLPLLKCCGSLTAIELDRDLIAPL 99
Query: 81 RNEYS-WNNVEIIQGDAVKVEWPSFNK-------VVSNIPYQISSPFTFKLLKMEFERAV 132
+ ++II D + V+ K +V N+PY ISSP F +L+ + A+
Sbjct: 100 TAAATPLGKLDIIHRDVLTVDLSILAKPGNKKLRLVGNLPYNISSPILFHVLQ---QAAI 156
Query: 133 V-----MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALV 187
+ M Q E RM A PG + Y RLS+M QA +V + + AF P PKV+SA+
Sbjct: 157 IADMHFMLQKEVVDRMAAPPGSKVYGRLSVMLQAWCEVTTMFVVPPDAFQPPPKVNSAIT 216
Query: 188 LITPK-KDRIELNES-----LVKALFQHRRKVVSKALRE 220
+ P+ I + ++ +V+A F RRK + +L +
Sbjct: 217 RLVPRDPTTIRIADTKRFSDIVRAAFGQRRKTLRNSLAD 255
>sp|P45438|ERMK_BACLI RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERYTHROMYCIN RESISTANCE PROTEIN)
>gi|80107|pir||B42473 rRNA methylase ermK - Bacillus
licheniformis >gi|143195|gb|AAA22595.1| (M77505)
erythromycin-inducible 23S ribosomal RNA methylase
[Bacillus licheniformis]
Length = 287
Score = 101 bits (250), Expect = 7e-21
Identities = 69/179 (38%), Positives = 98/179 (54%), Gaps = 8/179 (4%)
Query: 21 GQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEIL 80
GQH + K +I++ ++ A +S D VLE+G G G LT LS++A KV +E D + + IL
Sbjct: 23 GQHLMHNKKLIEEIVDRANISIDDTVLELGAGKGALTTMLSQKAGKVLAVENDSKFVAIL 82
Query: 81 -RNEYSWNNVEIIQGDAVKVEWPSFN-KVVSNIPYQISSPFTFKLLK---MEFERAVVMY 135
R N +II D +K+ P VVSNIPY I++P LL F++ +++
Sbjct: 83 TRKTAQHPNTKIIHQDIMKIHLPKEKFVVVSNIPYAITTPIMKMLLNNPASGFQKGIIVM 142
Query: 136 QLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPKKD 194
+ A R T+K +Y L + D+ IV I K F P PKVDSA+V IT KKD
Sbjct: 143 EKGAAKRFTSKFIKNSY---VLAWRMWFDIGIVREISKEHFSPPPKVDSAMVRITRKKD 198
>pir||T51591 dimethyladenosine transferase (EC 2.1.1.-) PFC1 [validated] -
Arabidopsis thaliana >gi|3005590|gb|AAC09322.1|
(AF051326) dimethyladenosine transferase [Arabidopsis
thaliana]
Length = 343
Score = 101 bits (250), Expect = 7e-21
Identities = 79/290 (27%), Positives = 150/290 (51%), Gaps = 41/290 (14%)
Query: 9 LSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVF 68
L+ G P +GQH+++ D+ D+ A+V + D VLE+GPG G LT+ L V
Sbjct: 64 LNSRGRFPRKSLGQHYMLNSDINDQLASAADVKEGDFVLEIGPGTGSLTNVLINLGATVL 123
Query: 69 TIELDRRIIEILRNEYSWNN-VEIIQGDAVKVEWPS-----------------FNKVVSN 110
IE D +++++ ++ ++ +++Q D VK S KVVSN
Sbjct: 124 AIEKDPHMVDLVSERFAGSDKFKVLQEDFVKCHIRSHMLSILETRRLSHPDSALAKVVSN 183
Query: 111 IPYQISSPFTFKLLKME--FERAVVMYQLEFALRMTAKPGDR--NYSRLSLMTQALADVE 166
+P+ IS+ LL M F + V++ Q E ALR+ +P R Y ++++ ++ E
Sbjct: 184 LPFNISTDVVKLLLPMGDIFSKVVLLLQDEAALRL-VEPALRTSEYRPINILINFYSEPE 242
Query: 167 IVMRIGKGAFYPKPKVDSALVLITPK--KDRIELNE-----SLVKALFQHRRKVVSKALR 219
R+ + F+P+PKVD+A+V K +D +++ SLV + F +RK++ K+L+
Sbjct: 243 YNFRVPRENFFPQPKVDAAVVTFKLKHPRDYPDVSSTKNFFSLVNSAFNGKRKMLRKSLQ 302
Query: 220 ESAHMLGIKDVKTVKDILSSVPHSNKRVFHLTPEEVKEIEEYLREHRIIS 269
H+ D++ + + +P +++ PEE+ ++++++ H +I+
Sbjct: 303 ---HISSSPDIEKALGV-AGLPATSR------PEEL-TLDDFVKLHNVIA 341
>emb|CAC12422.1| (AL445067) rRNA (adenine-N6, N6-)-dimethyltransferase (DIM1, yeast)
related protein [Thermoplasma acidophilum]
Length = 233
Score = 101 bits (249), Expect = 1e-20
Identities = 67/200 (33%), Positives = 114/200 (56%), Gaps = 10/200 (5%)
Query: 20 IGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEI 79
+GQ FL + + + +++ + + VLE+GPG G LT L +R K+ +E DR I
Sbjct: 1 MGQVFLQSRRIAEYEVDL--LGEPGTVLEIGPGHGVLTKILVERGFKITAVEKDRYIFGE 58
Query: 80 LRNEYSWNNVEIIQGDAVKVEWPSFNKVVSNIPYQISSPFTFKLLKMEFERAVVMYQLEF 139
L++ + N+ +I D + + S++ ++ NIPY ISSP FKL + EF R+V+M Q EF
Sbjct: 59 LQSLRA-ANLNLINMDFLDMAPGSYDYIIGNIPYSISSPIVFKLYEFEFRRSVIMVQKEF 117
Query: 140 ALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPKKDR---- 195
A ++ A P D SRL + +VE+ + + F P+P+VDSA++++ KK
Sbjct: 118 AEKI-AFPDD--MSRLYVNAHVRYNVELKRYVSRKNFNPQPEVDSAILVLEKKKYEEPYP 174
Query: 196 IELNESLVKALFQHRRKVVS 215
+E + ++ +F +RK +S
Sbjct: 175 LEFLDGVLVQMFSKKRKKLS 194
>sp|P44749|KSGA_HAEIN DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLMETHIONINE-6-N',
N'-ADENOSYL(RRNA) DIMETHYLTRANSFERASE) (16S RRNA
DIMETHYLASE) (HIGH LEVEL KASUGAMYCIN RESISTANCE PROTEIN
KSGA) (KASUGAMYCIN DIMETHYLTRANSFERASE)
>gi|1073774|pir||I64076 rRNA
(adenine-N6,N6-)-dimethyltransferase (EC 2.1.1.-) -
Haemophilus influenzae (strain Rd KW20)
>gi|1573534|gb|AAC22207.1| (U32736) dimethyladenosine
transferase (ksgA) [Haemophilus influenzae Rd]
Length = 287
Score = 99.1 bits (243), Expect = 5e-20
Identities = 70/222 (31%), Positives = 106/222 (47%), Gaps = 23/222 (10%)
Query: 19 RIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIE 78
R GQ+FL VI + + ++E+GPGLG LT+ + + + +ELDR + E
Sbjct: 14 RFGQNFLHDTSVIQGIVAAIYPQPNQFLVEIGPGLGALTEPVGELVDHLTVVELDRDLAE 73
Query: 79 ILRNE-YSWNNVEIIQGDAVKVEWPSFN------------KVVSNIPYQISSPFTFKLLK 125
LR+ + + +I+ DA++ ++ + +V N+PY IS+P F L K
Sbjct: 74 RLRHHPFLHQKLTVIETDAMQFDFGALYTKENLAEKGQKLRVFGNLPYNISTPLMFHLFK 133
Query: 126 ME--FERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVD 183
+ M Q E R+ A P + Y RL++M Q V V+ + AF P PKVD
Sbjct: 134 YHDVIQDMHFMLQKEVVKRLCAAPNSKAYGRLTIMAQYFCQVMPVLEVPPSAFKPAPKVD 193
Query: 184 SALVLITPK-------KDRIELNESLVKALFQHRRKVVSKAL 218
SA+V + P KD LN +A F RRK + AL
Sbjct: 194 SAVVRLIPHKELPHPVKDLYWLNRVCSQA-FNQRRKTLRNAL 234
>emb|CAB60001.1| (X51891) N-6-aminoadenine-N-methyltransferase [Saccharopolyspora
erythraea]
Length = 381
Score = 97.1 bits (238), Expect = 2e-19
Identities = 71/202 (35%), Positives = 109/202 (53%), Gaps = 11/202 (5%)
Query: 15 RPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDR 74
R + GQ+FL + I + E AE+ VLE GPG G LT EL+ RA++V + E+D
Sbjct: 34 RNRRQFGQNFLRDRKTIARIAETAELRPDLPVLEAGPGEGLLTRELADRARQVTSYEIDP 93
Query: 75 RIIEILRNEYSWN-NVEIIQGDAVKVEWPSFN-KVVSNIPYQISSPFTFKLLKM-EFERA 131
R+ + LR + S + N+E++ D + E P V IPY I+S L+ E A
Sbjct: 94 RLAKSLREKLSGHPNIEVVNADFLTAEPPPEPFAFVGAIPYGITSAIVDWCLEAPTIETA 153
Query: 132 VVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITP 191
++ QLEFA + T G +SRL++MT L + E V ++ + F P PKVDSA++ +
Sbjct: 154 TMVTQLEFARKRTGDYG--RWSRLTVMTWPLFEWEFVEKVDRRLFKPVPKVDSAIMRLRR 211
Query: 192 KKD------RIELNESLVKALF 207
+ + +E ES+V+ F
Sbjct: 212 RAEPLLEGAALERYESMVELCF 233
>sp|P06992|KSGA_ECOLI DIMETHYLADENOSINE TRANSFERASE (S-ADENOSYLMETHIONINE-6-N',
N'-ADENOSYL(RRNA) DIMETHYLTRANSFERASE) (16S RRNA
DIMETHYLASE) (HIGH LEVEL KASUGAMYCIN RESISTANCE PROTEIN
KSGA) (KASUGAMYCIN DIMETHYLTRANSFERASE)
>gi|66453|pir||XYECRO rRNA
(adenine-N6,N6-)-dimethyltransferase (EC 2.1.1.-) -
Escherichia coli >gi|40917|emb|CAA28417.1| (X04711) put.
16S rRNA methyltransferase (ksgA) (AA 1-273)
[Escherichia coli] >gi|146571|gb|AAA24049.1| (M11054)
methyltransferase (ksgA) [Escherichia coli]
>gi|216476|dbj|BAA01327.1| (D10483) rRNA
(adenosine-N6,N6-)-dimethyltransferase [Escherichia
coli] >gi|1786236|gb|AAC73162.1| (AE000115)
S-adenosylmethionine-6-N',N'-adenosyl (rRNA)
dimethyltransferase [Escherichia coli K12]
>gi|224747|prf||1112172A methyltransferase [Escherichia
coli]
Length = 273
Score = 96.7 bits (237), Expect = 3e-19
Identities = 69/217 (31%), Positives = 103/217 (46%), Gaps = 18/217 (8%)
Query: 19 RIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIE 78
R GQ+FL + VID + K ++E+GPGL LT+ + +R ++ IELDR +
Sbjct: 14 RFGQNFLNDQFVIDSIVSAINPQKGQAMVEIGPGLAALTEPVGERLDQLTVIELDRDLAA 73
Query: 79 ILRNE-YSWNNVEIIQGDAVKVEWPSFN-------KVVSNIPYQISSPFTFKLLKMEFER 130
L+ + + I Q DA+ + +V N+PY IS+P F L
Sbjct: 74 RLQTHPFLGPKLTIYQQDAMTFNFGELAEKMGQPLRVFGNLPYNISTPLMFHLFSYTDAI 133
Query: 131 AVV--MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVL 188
A + M Q E R+ A P + Y RLS+M Q +V V+ + AF P PKVDSA+V
Sbjct: 134 ADMHFMLQKEVVNRLVAGPNSKAYGRLSVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVR 193
Query: 189 ITPK-------KDRIELNESLVKALFQHRRKVVSKAL 218
+ P KD + + + F RRK + +L
Sbjct: 194 LVPHATMPHPVKD-VRVLSRITTEAFNQRRKTIRNSL 229
>sp|P06571|ERMG_BACSH RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERYTHROMYCIN RESISTANCE PROTEIN)
>gi|80191|pir||C26930 rRNA
(adenine-N6-)-methyltransferase (EC 2.1.1.48) ermG -
Bacillus sphaericus >gi|142884|gb|AAA22419.1| (M15332)
rRNA methyltransferase [Bacillus sphaericus]
Length = 244
Score = 96.3 bits (236), Expect = 3e-19
Identities = 62/187 (33%), Positives = 101/187 (53%), Gaps = 7/187 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+F+ K I+K + + + D + E+G G G T EL KR V IE+D ++
Sbjct: 5 NIKDSQNFITSKYHIEKIMNCISLDEKDNIFEIGAGKGHFTAELVKRCNFVTAIEIDSKL 64
Query: 77 IEILRNE-YSWNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
E+ RN+ ++ N +I+ D +K +PS N K+ +IPY IS T + K+ FE +
Sbjct: 65 CEVTRNKLLNYPNYQIVNDDILKFTFPSHNPYKIFGSIPYNIS---TNIIRKIVFESSAT 121
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPKK 193
+ L + D N S L+L+ A D+ I+ +I + F+PKPKVDSAL+++ K
Sbjct: 122 ISYLIVEYGFAKRLLDTNRS-LALLLMAEVDISILAKIPRYYFHPKPKVDSALIVLKRKP 180
Query: 194 DRIELNE 200
++ E
Sbjct: 181 AKMAFKE 187
>gi|11416997 putative dimethyladenosine transferase [Homo sapiens]
Length = 381
Score = 94.8 bits (232), Expect = 1e-18
Identities = 53/113 (46%), Positives = 73/113 (63%), Gaps = 6/113 (5%)
Query: 116 SSPFTFKLL--KMEFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGK 173
SSPF FKLL + F A++M+Q EFALR+ AKPGD+ Y RLS+ TQ LA V+ +M++GK
Sbjct: 202 SSPFVFKLLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGK 261
Query: 174 GAFYPKPKVDSALVLITPKKDRIELN----ESLVKALFQHRRKVVSKALRESA 222
F P PKV+S++V I PK +N + LV+ F + K +S A + SA
Sbjct: 262 NNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSA 314
>gb|AAC37034.1| (L42817) rRNA methyltransferase [Bacteroides thetaiotaomicron]
Length = 244
Score = 93.6 bits (229), Expect = 2e-18
Identities = 61/187 (32%), Positives = 99/187 (52%), Gaps = 7/187 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+F+ K I+K + + + D + E+G G G T EL KR V IE+D ++
Sbjct: 5 NIKDSQNFITSKYHIEKIMNCISLDEKDNIFEIGAGKGHFTAELVKRCNFVTAIEIDSKL 64
Query: 77 IEILRNE-YSWNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
E+ RN+ ++ N +I+ D +K +PS N K+ +IPY IS T + K+ FE +
Sbjct: 65 CEVTRNKLLNYPNYQIVNDDILKFTFPSHNPYKIFGSIPYNIS---TNIIRKIVFESSAT 121
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPKK 193
+ L D N S L+L+ A D+ I+ +I + F+PKPKVDS L+++ K
Sbjct: 122 ISYLIVEYGFAKMLLDTNRS-LALLLMAEVDISILAKIPRYYFHPKPKVDSTLIVLKRKP 180
Query: 194 DRIELNE 200
++ E
Sbjct: 181 AKMAFKE 187
>pir||F70456 dimethyladenosine transferase - Aquifex aeolicus
>gi|2984099|gb|AAC07637.1| (AE000757) dimethyladenosine
transferase [Aquifex aeolicus]
Length = 248
Score = 93.2 bits (228), Expect = 3e-18
Identities = 73/218 (33%), Positives = 110/218 (49%), Gaps = 16/218 (7%)
Query: 14 IRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKR-AKKVFTIEL 72
+R GQH L+ + V+ K E + + + V+EVG G G LT L + KK++ IEL
Sbjct: 2 VRLKKSFGQHLLVSEGVLKKIAEELNIEEGNTVVEVGGGTGNLTKVLLQHPLKKLYVIEL 61
Query: 73 DRRIIEILRNEYSWNNVEIIQGDAVKVEWPSFN---KVVSNIPYQISSPFTFKLL--KME 127
DR ++E L++ +E+I DA K + S KVV N+PY ++S + K
Sbjct: 62 DREMVENLKS-IGDERLEVINEDASKFPFCSLGKELKVVGNLPYNVASLIIENTVYNKDC 120
Query: 128 FERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALV 187
AV M Q E A ++ G ++ LS+ + DV VM + F P PKV SA++
Sbjct: 121 VPLAVFMVQKEVAEKL---QGKKDTGWLSVFVRTFYDVNYVMTVPPRFFVPPPKVQSAVI 177
Query: 188 LIT-----PKKDRIELNESLVKALFQHRRKVVSKALRE 220
+ P KD + L K +FQ+RRKV+ K + E
Sbjct: 178 KLVKNEKFPVKDLKNYKKFLTK-IFQNRRKVLRKKIPE 214
>sp|P10337|ERMF_BACFR RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERYTHROMYCIN RESISTANCE PROTEIN)
>gi|79389|pir||A25157
macrolide/lincosamide/streptogramin B resistance protein
- Bacteroides fragilis >gi|1085780|pir||S55397 probable
methyltransferase (EC 2.1.1.-) [similarity] -
Bacteroides fragilis plasmid >gi|150541|gb|AAA98217.1|
(M14730) macrolide-lincosamide-streptogramin
B-resistance protein [Bacillus fragilis]
>gi|154890|gb|AAA88675.1| (M17124)
macrolide-lincosamide-streptogramin-resistance protein
[Transposon Tn4351] >gi|853782|emb|CAA60706.1| (X87253)
ermF [Bacteroides sp.] >gi|1016682|gb|AAB39962.1|
(U30830) rRNA methyltransferase [Cloning vector pFD288]
Length = 266
Score = 92.8 bits (227), Expect = 4e-18
Identities = 67/178 (37%), Positives = 95/178 (52%), Gaps = 10/178 (5%)
Query: 21 GQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEIL 80
GQHF I K +I AI A +S D VL++G G GFLT L K A V IE D ++E L
Sbjct: 12 GQHFTIDKVLIKDAIRQANISNQDTVLDIGAGKGFLTVHLLKIANNVVAIENDTALVEHL 71
Query: 81 RNEYS-WNNVEIIQGDAVKVEWPSFN-KVVSNIPYQISSPFTFKLLKME----FERAVVM 134
R +S NV+++ D P F KVVSNIPY I+S FK+L E F ++
Sbjct: 72 RKLFSDARNVQVVGCDFRNFAVPKFPFKVVSNIPYGITSDI-FKILMFESLGNFLGGSIV 130
Query: 135 YQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPK 192
QLE ++ ++ + Y+ ++ D+++V +G +F P P V SAL+ I K
Sbjct: 131 LQLEPTQKLFSR---KLYNPYTVFYHTFFDLKLVYEVGPESFLPPPTVKSALLNIKRK 185
>gb|AAA27431.1| (M17808) clindamycin resistance determinant (ermFS) [Transposon
Tn4551] >gi|6979874|gb|AAF34636.1| (AF219231) ErmF
[Shuttle vector pHS17]
Length = 266
Score = 92.8 bits (227), Expect = 4e-18
Identities = 67/178 (37%), Positives = 95/178 (52%), Gaps = 10/178 (5%)
Query: 21 GQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEIL 80
GQHF I K +I AI A +S D VL++G G GFLT L K A V IE D ++E L
Sbjct: 12 GQHFTIDKVLIKDAIRQANISNQDTVLDIGAGKGFLTVHLLKIANNVVAIENDTALVEHL 71
Query: 81 RNEYS-WNNVEIIQGDAVKVEWPSFN-KVVSNIPYQISSPFTFKLLKME----FERAVVM 134
R +S NV+++ D P F KVVSNIPY I+S FK+L E F ++
Sbjct: 72 RKLFSDARNVQVVGCDFRNFAVPKFPFKVVSNIPYGITSDI-FKILMFESLGNFLGGSIV 130
Query: 135 YQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPK 192
QLE ++ ++ + Y+ ++ D+++V +G +F P P V SAL+ I K
Sbjct: 131 LQLEPTQKLFSR---KLYNPYTVFYHTFFDLKLVYEVGPESFLPPPTVKSALLNIKRK 185
>pir||T29195 hypothetical protein T03F1.7 - Caenorhabditis elegans
>gi|1825610|gb|AAB42235.1| (U88169) similar to the rRNA
adenine N-6-methytransferase family [Caenorhabditis
elegans]
Length = 313
Score = 92.8 bits (227), Expect = 4e-18
Identities = 70/246 (28%), Positives = 124/246 (49%), Gaps = 38/246 (15%)
Query: 12 YGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDE-LSKRAKKVFTI 70
Y +R + Q++L+ ++ K + A+V + D V+E+GPG G +T L A ++ +
Sbjct: 20 YRLRAKKILSQNYLMDMNITRKIAKHAKVIEKDWVIEIGPGPGGITRAILEAGASRLDVV 79
Query: 71 ELDRRIIEILRN--EYSWNNVEIIQGDAVK----------------VEWPSFN----KVV 108
E+D R I L++ E + + + I DA++ V+W N V+
Sbjct: 80 EIDNRFIPPLQHLAEAADSRMFIHHQDALRTEIGDIWKNETARPESVDWHDSNLPAMHVI 139
Query: 109 SNIPYQISSPFTFKLLK-MEFERAVVMY---------QLEFALRMTAKPGDRNYSRLSLM 158
N+P+ I+SP K L+ M + R V Y QLE A R+ + SR+S+M
Sbjct: 140 GNLPFNIASPLIIKYLRDMSYRRGVWQYGRVPLTLTFQLEVAKRLCSPIACDTRSRISIM 199
Query: 159 TQALADVEIVMRIGKGAFYPKPKVDSALVLITPKKDRI-----ELNESLVKALFQHRRKV 213
+Q +A+ ++V +I F P+P+VD +V P+K + E+ E + + +F +R+K
Sbjct: 200 SQYVAEPKMVFQISGSCFVPRPQVDVGVVRFVPRKTPLVNTSFEVLEKVCRQVFHYRQKY 259
Query: 214 VSKALR 219
V+K L+
Sbjct: 260 VTKGLK 265
>gb|AAF68230.1|AF203972_3 (AF203972) Shuttle vector pBA complete sequence
Length = 266
Score = 92.8 bits (227), Expect = 4e-18
Identities = 67/178 (37%), Positives = 95/178 (52%), Gaps = 10/178 (5%)
Query: 21 GQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEIL 80
GQHF I K +I AI A +S D VL++G G GFLT L K A V IE D ++E L
Sbjct: 12 GQHFTIDKVLIKDAIRQANISNQDTVLDIGAGKGFLTVHLLKIANNVVAIENDTALVEHL 71
Query: 81 RNEYS-WNNVEIIQGDAVKVEWPSFN-KVVSNIPYQISSPFTFKLLKME----FERAVVM 134
R +S NV+++ D P F KVVSNIPY I+S FK+L E F ++
Sbjct: 72 RKLFSDARNVQVVGCDFRNFAVPKFPFKVVSNIPYGITSDI-FKILMFESLGNFLGGSIV 130
Query: 135 YQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPK 192
QLE ++ ++ + Y+ ++ D+++V +G +F P P V SAL+ I K
Sbjct: 131 LQLEPTQKLFSR---KLYNPYTVFYHTFFDLKLVYEVGPESFLPPPTVKSALLNIKRK 185
>sp|Q02607|ERMU_BACFR RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERYTHROMYCIN RESISTANCE PROTEIN)
>gi|421315|pir||S34413 methyltransferase - Bacteroides
fragilis >gi|143943|gb|AAA63165.1| (M62487)
methyltransferase [Bacteroides fragilis]
Length = 266
Score = 91.3 bits (223), Expect = 1e-17
Identities = 66/178 (37%), Positives = 94/178 (52%), Gaps = 10/178 (5%)
Query: 21 GQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEIL 80
GQHF I K +I AI A +S D VL++G G GFLT L K A V IE D ++E L
Sbjct: 12 GQHFTIDKVLIKDAIRQANISNQDTVLDIGAGKGFLTVHLLKIANNVVAIENDTALVEHL 71
Query: 81 RNEYS-WNNVEIIQGDAVKVEWPSFN-KVVSNIPYQISSPFTFKLLKME----FERAVVM 134
R +S NV+++ D P F KVVSNIPY I+S FK+L E F ++
Sbjct: 72 RKLFSDARNVQVVGCDFRNFAVPKFPFKVVSNIPYGITSDI-FKILMFENLENFLGGSIV 130
Query: 135 YQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPK 192
Q E ++ ++ + Y+ ++ D+++V +G +F P P V SAL+ I K
Sbjct: 131 LQFEPTQKLFSR---KLYNPYTVFYHTFFDLKLVYEVGPESFLPPPTVKSALLNIKRK 185
>pir||G71337 probable dimethyladenosine transferase (ksgA) - syphilis spirochete
>gi|3322613|gb|AAC65323.1| (AE001213) dimethyladenosine
transferase (ksgA) [Treponema pallidum]
Length = 285
Score = 90.1 bits (220), Expect = 2e-17
Identities = 63/224 (28%), Positives = 117/224 (52%), Gaps = 17/224 (7%)
Query: 9 LSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVF 68
L++ G+R + + GQ+FL+ + + +++ + + V E+G G+G +T L + + +
Sbjct: 16 LTERGLRMHKKWGQNFLLDPVLRTQLVKILAPERGERVWEIGAGIGAMTALLVQNSDFLT 75
Query: 69 TIELDRRIIEILRNEYSWNNVEIIQGDAVKVEWPSFNK------VVSNIPYQISSPFTFK 122
E+DR ++ LR + +V +I+GD ++ +W + V+ N+PY I++ F
Sbjct: 76 VFEIDRGFVQTLRKLFD-AHVRVIEGDVLQ-QWHAAAAQEQPACVLGNLPYNIAARFIGN 133
Query: 123 LLKME--FERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKP 180
++ F+R VV Q E LRMTA P + YS S++ Q +V ++ + F+P+P
Sbjct: 134 TIESGYIFKRMVVTVQKEIGLRMTALPAQKWYSYFSVLCQWQYEVRVIRNVAPVCFWPRP 193
Query: 181 KVDSALVLITPKKDRI------ELNESLVKALFQHRRKVVSKAL 218
V S +++T K++ + L + K LF RRK V L
Sbjct: 194 HVVSQALVLT-KRNAVPSCVDPALFLHVTKTLFSARRKTVRNNL 236
>gb|AAC36915.1| (L22689) 23S rRNA methlyase [Clostridium perfringens]
>gi|1092864|prf||2102192A 23S rRNA methylase
[Clostridium perfringens]
Length = 257
Score = 89.7 bits (219), Expect = 3e-17
Identities = 66/206 (32%), Positives = 101/206 (48%), Gaps = 9/206 (4%)
Query: 20 IGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEI 79
+ Q+F+ K+ I K I+ +SK+D V+E+GPG G +T+ L +++ V IELDR +
Sbjct: 16 VSQNFITSKNTIYKLIKKTNISKNDFVIEIGPGKGHITEALCEKSYWVTAIELDRSLYGN 75
Query: 80 LRNEY-SWNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVVMYQ 136
L N++ S NNV +I D + + P KV SNIP+ I++ KLL E M+
Sbjct: 76 LINKFKSKNNVTLINKDFLNWKLPKKREYKVFSNIPFYITTKIIKKLLLEELNSPTDMWL 135
Query: 137 LEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPK---- 192
+ G S+LSL+ + D++IV + F+P P VD LV K
Sbjct: 136 VMEKGSAKRFMGIPRESKLSLLLKTKFDIKIVHYFNREDFHPMPSVDCVLVYFKRKYKYD 195
Query: 193 --KDRIELNESLVKALFQHRRKVVSK 216
KD S + + R V +K
Sbjct: 196 ISKDEWNEYTSFISKSINNLRDVFTK 221
>pir||C81269 probable dimethyladenosine transferase (16S rRNA dimethylase) (EC
2.1.1.-) Cj1711c [imported] - Campylobacter jejuni
(strain NCTC 11168) >gi|6969126|emb|CAB73697.1|
(AL139079) putative dimethyladenosine transferase (16S
rRNA dimethylase) [Campylobacter jejuni]
Length = 266
Score = 87.0 bits (212), Expect = 2e-16
Identities = 65/213 (30%), Positives = 110/213 (51%), Gaps = 16/213 (7%)
Query: 14 IRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELD 73
++ + GQ+FLI K V+ K I+ ++++ E+GPGLG LT EL K +V E+D
Sbjct: 2 VKAKKQYGQNFLIDKSVLAKIIQAIPKEMNNII-EIGPGLGDLTQELLK-ISQVKAYEID 59
Query: 74 RRIIEILRNEYS----WNNVEIIQGDAVKVEWPSFNK----VVSNIPYQISSPFTFKLLK 125
+I IL+ ++ +I DA + PS ++ +V+N+PY ++S K L+
Sbjct: 60 NDLIPILKKKFQKELECGKFNLIHQDASEAFNPSLDEKPYFLVANLPYYVASHIILKALE 119
Query: 126 -MEFERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDS 184
+VM Q E A + AK G+ +S L +++ + + +I+ + F P PKV S
Sbjct: 120 DKNCLGLIVMAQREMAEKFCAKEGNSEFSSLGVLSAMICERKILFDVDPQCFNPPPKVMS 179
Query: 185 ALVLITPKKDRIELNE-----SLVKALFQHRRK 212
A++ + KD EL E + +K F+ RK
Sbjct: 180 AVMSLIKTKDFDELCEIENFKNFLKDCFKAPRK 212
>gi|9507344 adenine methylase (ermC) [Plasmid pE194]
>gi|127193|sp|P02979|ERM2_STAAU RRNA ADENINE
N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERYTHROMYCIN RESISTANCE PROTEIN)
>gi|66452|pir||YESA9E rRNA
(adenine-N6-)-methyltransferase (EC 2.1.1.48) -
Staphylococcus aureus plasmids >gi|46558|emb|CAA24591.1|
(V01278) reading frame B mls resistance [Staphylococcus
aureus] >gi|209127|gb|AAA72303.1| (L05081) erythromycin
resistance determinant [unidentified cloning vector]
>gi|455314|gb|AAB59079.1| (J01755) adenine methylase
(ermC) [Plasmid pE194] >gi|1185576|gb|AAB40308.1|
(U46198) erythromycin resistance; erythromycin
resistance determinant [Cloning vector pDG1728]
>gi|1185582|gb|AAB40313.1| (U46199) erythromycin
resistance; erythromycin resistance determinant
[Cloning vector pDG1730] >gi|1185608|gb|AAB40336.1|
(U46202) erythromycin resistance; erythromycin
resistance determinant [Cloning vector pDG1729]
>gi|1185616|gb|AAB40343.1| (U46203) erythromicin
resistance; erythromycin resistance determinant
[Cloning vector pDG1731] >gi|1638844|emb|CAA57985.1|
(X82668) adenine methylase [Staphylococcus equorum]
>gi|5327232|emb|CAB46341.1| (Y17294) adenine methylase
[Staphylococcus aureus] >gi|11545512|gb|AAG37883.1|
(AF307747) erythromycin resistance [Tn10 delivery vector
pHV1248] >gi|11545518|gb|AAG37888.1| (AF307748)
erythromycin resistance [Tn10 delivery vector pHV1249]
Length = 244
Score = 85.0 bits (207), Expect = 8e-16
Identities = 61/228 (26%), Positives = 114/228 (49%), Gaps = 16/228 (7%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+F+ K IDK + +++ D + E+G G G T EL KR V IE+D ++
Sbjct: 5 NIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFTLELVKRCNFVTAIEIDHKL 64
Query: 77 IEILRNE-YSWNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAV- 132
+ N+ +N +++ D ++ ++P K+ NIPY IS+ K++ +
Sbjct: 65 CKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPYNISTDIIRKIVFDSIANEIY 124
Query: 133 VMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPK 192
++ + FA R+ L+L+ A D+ I+ + + F+PKPKV+S+L+ ++ K
Sbjct: 125 LIVEYGFAKRLL-----NTKRSLALLLMAEVDISILSMVPREYFHPKPKVNSSLIRLSRK 179
Query: 193 KDRI------ELNESLVKALFQHRRKVVSK-ALRESAHMLGIKDVKTV 233
K RI + N ++K + + +K+ +K S GI D+ +
Sbjct: 180 KSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGIDDLNNI 227
>gb|AAD46055.1|AF076212_5 (AF076212) adenine methylase [Promoter screenings vector pMM223]
>gi|5668900|gb|AAD46060.1|AF076213_5 (AF076213) adenine
methylase [Promoter screenings vector pMM225]
Length = 244
Score = 85.0 bits (207), Expect = 8e-16
Identities = 61/228 (26%), Positives = 114/228 (49%), Gaps = 16/228 (7%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+F+ K IDK + +++ D + E+G G G T EL KR V IE+D ++
Sbjct: 5 NIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFTLELVKRCNFVTAIEIDHKL 64
Query: 77 IEILRNE-YSWNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAV- 132
+ N+ +N +++ D ++ ++P K+ NIPY IS+ K++ +
Sbjct: 65 CKTTENKLVDHDNFQVLNKDIMQFKFPKNQSYKIYGNIPYNISTDIIRKIVFDSIANEIY 124
Query: 133 VMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPK 192
++ + FA R+ L+L+ A D+ I+ + + F+PKPKV+S+L+ ++ K
Sbjct: 125 LIVEYGFAKRLL-----NTKRSLALLLMAEVDISILSMVPREYFHPKPKVNSSLIRLSRK 179
Query: 193 KDRI------ELNESLVKALFQHRRKVVSK-ALRESAHMLGIKDVKTV 233
K RI + N ++K + + +K+ +K S GI D+ +
Sbjct: 180 KSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGIDDLNNI 227
>pir||E70603 probable dimethyladenosine transferase - Mycobacterium tuberculosis
(strain H37RV) >gi|2052147|emb|CAB08137.1| (Z94752) ksgA
[Mycobacterium tuberculosis]
Length = 317
Score = 84.3 bits (205), Expect = 1e-15
Identities = 71/235 (30%), Positives = 106/235 (44%), Gaps = 30/235 (12%)
Query: 8 LLSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKV 67
L + RP +GQ+F+ + + + + + VS+SD+VLEVGPGLG LT L R V
Sbjct: 22 LAKELDFRPRKSLGQNFVHDANTVRRVVAASGVSRSDLVLEVGPGLGSLTLALLDRGATV 81
Query: 68 FTIELDRRIIEILRN---EYSWNNVE---IIQGDAVKVEWPSF----NKVVSNIPYQISS 117
+E+D + L+ E+S + V ++ D + + VV+N+PY ++
Sbjct: 82 TAVEIDPLLASRLQQTVAEHSHSEVHRLTVVNRDVLALRREDLAAAPTAVVANLPYNVAV 141
Query: 118 PFTFKLLKMEFER---AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKG 174
P LL +EF VM Q E A R+ A+PG + Y S+ + V +
Sbjct: 142 PALLHLL-VEFPSIRVVTVMVQAEVAERLAAEPGSKEYGVPSVKLRFFGRVRRCGMVSPT 200
Query: 175 AFYPKPKVDSALVLITPKKDRIELNE------------SLVKALFQHRRKVVSKA 217
F+P P+V S LV I DR E + LV F RRK A
Sbjct: 201 VFWPIPRVYSGLVRI----DRYETSPWPTDDAFRRRVFELVDIAFAQRRKTSRNA 251
>gi|7705785 CGI-75 protein [Homo sapiens] >gi|4929619|gb|AAD34070.1|AF151833_1
(AF151833) CGI-75 protein [Homo sapiens]
Length = 346
Score = 83.9 bits (204), Expect = 2e-15
Identities = 72/235 (30%), Positives = 114/235 (47%), Gaps = 33/235 (14%)
Query: 18 DRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDE-LSKRAKKVFTIELDRRI 76
+ + Q+FL+ + DK + A + V EVGPG G +T L+ ++ +E D R
Sbjct: 31 NELSQNFLLDLRLTDKIVRKAGNLTNAYVYEVGPGPGGITRSILNADVAELLVVEKDTRF 90
Query: 77 IEILR--NEYSWNNVEIIQGDAV--KVE----------W---PSFNKVVSNIPYQISSPF 119
I L+ ++ + + I+ GD + KVE W P ++ N+P+ +S+P
Sbjct: 91 IPGLQMLSDAAPGKLRIVHGDVLTFKVEKAFSESLKRPWEDDPPNVHIIGNLPFSVSTPL 150
Query: 120 TFKLLKMEFER----------AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVM 169
K L+ R + +Q E A R+ A G + SRLS+M Q L +V +
Sbjct: 151 IIKWLENISCRDGPFVYGRTQMTLTFQKEVAERLAANTGSKQRSRLSVMAQYLCNVRHIF 210
Query: 170 RIGKGAFYPKPKVDSALV----LITPKKDR-IELNESLVKALFQHRRKVVSKALR 219
I AF PKP+VD +V LI PK ++ +L E +V+ +FQ RRK + LR
Sbjct: 211 TIPGQAFVPKPEVDVGVVHFTPLIQPKIEQPFKLVEKVVQNVFQFRRKYCHRGLR 265
>gi|11417701 similar to CGI-75 protein (H. sapiens) [Homo sapiens]
>gi|11418596|ref|XP_004314.1| similar to CGI-75 protein
(H. sapiens) [Homo sapiens]
Length = 346
Score = 83.9 bits (204), Expect = 2e-15
Identities = 72/234 (30%), Positives = 114/234 (47%), Gaps = 33/234 (14%)
Query: 19 RIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDE-LSKRAKKVFTIELDRRII 77
++ Q+FL+ + DK + A + V EVGPG G +T L+ ++ +E D R I
Sbjct: 32 QLSQNFLLDLRLTDKIVRKAGNLTNAYVYEVGPGPGGITRSILNADVAELLVVEKDTRFI 91
Query: 78 EILR--NEYSWNNVEIIQGDAV--KVE----------W---PSFNKVVSNIPYQISSPFT 120
L+ ++ + + I+ GD + KVE W P ++ N+P+ +S+P
Sbjct: 92 PGLQMLSDAAPGKLRIVHGDVLTFKVEKAFSESLKRPWEDDPPNVHIIGNLPFSVSTPLI 151
Query: 121 FKLLKMEFER----------AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMR 170
K L+ R + +Q E A R+ A G + SRLS+M Q L +V +
Sbjct: 152 IKWLENISCRDGPFVYGRTQMTLTFQKEVAERLAANTGSKQRSRLSVMAQYLCNVRHIFT 211
Query: 171 IGKGAFYPKPKVDSALV----LITPKKDR-IELNESLVKALFQHRRKVVSKALR 219
I AF PKP+VD +V LI PK ++ +L E +V+ +FQ RRK + LR
Sbjct: 212 IPGQAFVPKPEVDVGVVHFTPLIQPKIEQPFKLVEKVVQNVFQFRRKYCHRGLR 265
>gb|AAG37071.1|AF310974_2 (AF310974) methylase ermT [Plasmid p121BS]
Length = 244
Score = 83.9 bits (204), Expect = 2e-15
Identities = 58/212 (27%), Positives = 113/212 (52%), Gaps = 15/212 (7%)
Query: 15 RPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDR 74
+ N + Q+F+ K I++ + ++ +D ++E+G G G T EL+KR V IE+D
Sbjct: 3 KKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHFTLELAKRCNYVTAIEIDP 62
Query: 75 RIIEILRNE-YSWNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLL-KMEFER 130
++ I +N+ + N ++I D ++ ++P K+ NIPY IS+ K++ +
Sbjct: 63 KLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNIPYNISTDIIRKIVFESTATE 122
Query: 131 AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLI- 189
+ ++ + FA R+ + N S L+L D+ I+ +I + F+PKP+V+S+L+++
Sbjct: 123 SYLIVEYGFAKRLL----NTNRS-LALFLMTEVDISILSKIPREYFHPKPRVNSSLIVLK 177
Query: 190 -----TPKKDRIELNESLVKALFQHRRKVVSK 216
KDR + ++K + + RK+ SK
Sbjct: 178 RHPSKISLKDRKQYENFVMKWVNKEYRKLFSK 209
>emb|CAA70207.1| (Y09002) adenine methylase [Staphylococcus haemolyticus]
Length = 244
Score = 83.5 bits (203), Expect = 2e-15
Identities = 61/228 (26%), Positives = 112/228 (48%), Gaps = 16/228 (7%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+F+ K IDK + +++ D + E+G G G T EL KR V IE+D ++
Sbjct: 5 NIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFTLELVKRCNFVTAIEIDHKL 64
Query: 77 IEILRNE-YSWNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEF-ERAV 132
+ N+ +N +++ D ++ ++P K+ NIPY IS+ K++ +
Sbjct: 65 CKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNIPYNISTDIIRKIVFDSIADEIY 124
Query: 133 VMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPK 192
++ + FA R+ L+L A D+ I+ + + F+PKPKV+S+L+ + K
Sbjct: 125 LIVEYGFAKRLL-----NTKRSLALFLMAEVDISILSMVPREYFHPKPKVNSSLIRLNRK 179
Query: 193 KDRI------ELNESLVKALFQHRRKVVSK-ALRESAHMLGIKDVKTV 233
K RI + N ++K + + +K+ +K S GI D+ +
Sbjct: 180 KSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGIDDLNNI 227
>gi|9507389 23S RNA methylase [Plasmid pT48] >gi|127195|sp|P13978|ERM4_STAAU
RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERYTHROMYCIN RESISTANCE PROTEIN)
>gi|538884|pir||B46568 ermC protein - Staphylococcus
aureus plasmid pT48 >gi|455358|gb|AAA20192.1| (M19652)
23S RNA methylase [Plasmid pT48]
Length = 244
Score = 82.7 bits (201), Expect = 4e-15
Identities = 60/228 (26%), Positives = 113/228 (49%), Gaps = 16/228 (7%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+F+ K IDK + +++ D + E+G G G T EL +R V IE+D ++
Sbjct: 5 NIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFTLELVQRCNFVTAIEIDHKL 64
Query: 77 IEILRNE-YSWNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEF-ERAV 132
+ N+ +N +++ D ++ ++P K+ NIPY IS+ K++ +
Sbjct: 65 CKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNIPYNISTDIIRKIVFDSIADEIY 124
Query: 133 VMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPK 192
++ + FA R+ L+L+ A D+ I+ + + F+PKPKV+S+L+ + K
Sbjct: 125 LIVEYGFAKRLL-----NTKRSLALLLMAEVDISILSMVPREYFHPKPKVNSSLIRLNRK 179
Query: 193 KDRI------ELNESLVKALFQHRRKVVSK-ALRESAHMLGIKDVKTV 233
K RI + N ++K + + +K+ +K S GI D+ +
Sbjct: 180 KSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGIDDLNNI 227
>pir||S42117 kasugamycin dimethyltransferase (EC 2.1.1.-) - Mycoplasma
capricolum >gi|416234|dbj|BAA03626.1| (D14983)
kasugamycin dimethyltransferase [Mycoplasma capricolum]
Length = 204
Score = 82.7 bits (201), Expect = 4e-15
Identities = 59/209 (28%), Positives = 108/209 (51%), Gaps = 26/209 (12%)
Query: 76 IIEILRNEYSWNNVEIIQGDAVKVEWPSFNK--------VVSNIPYQISSPFTFKLLKME 127
++EIL+ +++ +N+EIIQ D ++++ ++SN PY I+S FK L++
Sbjct: 1 MVEILKTKFNHSNLEIIQADVLEIDLKQLISKYDYKNISIISNTPYYITSEILFKTLQIS 60
Query: 128 --FERAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSA 185
+AV M Q E ALR+ + + NY+ LS+ Q + + K FYP PKVDSA
Sbjct: 61 DLLTKAVFMLQKEVALRICSNKNENNYNNLSIACQFYSQRNFEFVVNKKMFYPIPKVDSA 120
Query: 186 LVLITPKK-DRIELNE-----SLVKALFQHRRKVVSKALRESAHMLGIKDVKTVKDILSS 239
++ +T + ++N+ V+ LF ++RK + L I++ + L
Sbjct: 121 IISLTFNNIYKKQINDDKKFIEFVRTLFNNKRKTILNNLNNI-----IQNKNKALEYLKM 175
Query: 240 VPHSNKRVFHLTPEEVKEIEEYLREHRII 268
+ S+ +L PE++ +I+EY++ +I
Sbjct: 176 LNISS----NLRPEQL-DIDEYIKLFNLI 199
>gi|9507309 macrolide-lincosamide-streptogramin B-resistance protein [Plasmid
pIM13] >gi|127198|sp|P13956|ERM_BACSU RRNA ADENINE
N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERYTHROMYCIN RESISTANCE PROTEIN)
>gi|80331|pir||B25233
macrolide/lincosamide/streptogramin B resistance
methylase - Bacillus subtilis plasmid pIM13
>gi|11256781|pir||A81241 rRNA adenine
N-6-methyltransferase NMB0066 [imported] - Neisseria
meningitidis (group B strain MD58)
>gi|7546362|pdb|1QAN|A Chain A, The Structure Of The
Rrna Methyltransferase Ermc': Implications For The
Reaction Mechanism >gi|7546363|pdb|1QAO|A Chain A, The
Structure Of The Rrna Methyltransferase Ermc':
Implications For The Reaction Mechanism
>gi|7546364|pdb|1QAQ|A Chain A, The Structure Of The
Rrna Methyltransferase Ermc': Implications For The
Reaction Mechanism >gi|7546361|pdb|1QAM|A Chain A, The
Structure Of The Rrna Methyltransferase Ermc':
Implications For The Reaction Mechanism
>gi|4699789|pdb|2ERC|A Chain A, Crystal Structure Of
Ermc' A Rrna-Methyl Transferase >gi|4699790|pdb|2ERC|B
Chain B, Crystal Structure Of Ermc' A Rrna-Methyl
Transferase >gi|149052|gb|AAA98136.1| (M13761)
macrolide-lincosamide-streptogramin B-resistance protein
[Plasmid pIM13] >gi|7225287|gb|AAF40534.1| (AE002366)
rRNA adenine N-6-methyltransferase; foreign cassette
inserted to disrupt SiaD (NMB0067) to reduce virulence
[Neisseria meningitidis MC58]
Length = 244
Score = 81.9 bits (199), Expect = 7e-15
Identities = 60/228 (26%), Positives = 112/228 (48%), Gaps = 16/228 (7%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+F+ K IDK + +++ D + E+G G G T EL +R V IE+D ++
Sbjct: 5 NIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFTLELVQRCNFVTAIEIDHKL 64
Query: 77 IEILRNE-YSWNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEF-ERAV 132
+ N+ +N +++ D ++ ++P K+ NIPY IS+ K++ +
Sbjct: 65 CKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNIPYNISTDIIRKIVFDSIADEIY 124
Query: 133 VMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPK 192
++ + FA R+ L+L A D+ I+ + + F+PKPKV+S+L+ + K
Sbjct: 125 LIVEYGFAKRLL-----NTKRSLALFLMAEVDISILSMVPREYFHPKPKVNSSLIRLNRK 179
Query: 193 KDRI------ELNESLVKALFQHRRKVVSK-ALRESAHMLGIKDVKTV 233
K RI + N ++K + + +K+ +K S GI D+ +
Sbjct: 180 KSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGIDDLNNI 227
>emb|CAA70208.1| (Y09003) adenine methylase [Staphylococcus hyicus]
Length = 244
Score = 81.9 bits (199), Expect = 7e-15
Identities = 58/230 (25%), Positives = 118/230 (51%), Gaps = 16/230 (6%)
Query: 15 RPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDR 74
+ N + Q+F+ K +DK + +++ D + E+G G G T EL +R V IE+D
Sbjct: 3 KKNIKDSQNFITSKRNVDKIMTNISLNEHDNIFEIGSGKGHFTLELVQRCNFVTAIEIDH 62
Query: 75 RIIEILRNE-YSWNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLL-KMEFER 130
++ + N+ + +N +++ D ++ ++P K+ +IPY IS+ K++ + +
Sbjct: 63 KLCKTTENKLVNHDNFQVLNKDILQFKFPKNQSYKIFGSIPYNISTDIIRKVVFESIADE 122
Query: 131 AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ ++ + FA R+ L+L+ A D+ I+ + + F+PKPKV+S+L+ +
Sbjct: 123 SYLIVEYGFAKRLL-----NTKRSLALLLMAEVDISILSMVPREYFHPKPKVNSSLIRLN 177
Query: 191 PKKDRI------ELNESLVKALFQHRRKVVSK-ALRESAHMLGIKDVKTV 233
KK RI + N ++K + + +K+ +K +S GI D+ +
Sbjct: 178 RKKSRISYKDKQKYNYFVMKWVNKEYKKIFTKNQFNKSLKHAGIDDLNNI 227
>sp|P07287|ERME_SACER RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERYTHROMYCIN RESISTANCE PROTEIN) (NMT)
>gi|66451|pir||XYSMRE rRNA
(adenine-N6-)-methyltransferase (EC 2.1.1.48) -
Saccharopolyspora erythraea
Length = 370
Score = 81.5 bits (198), Expect = 9e-15
Identities = 68/202 (33%), Positives = 103/202 (50%), Gaps = 22/202 (10%)
Query: 15 RPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDR 74
R + GQ+FL + I + E AE+ VLE GPG G LT EL+ RA++V + E+D
Sbjct: 34 RNRRQFGQNFLRDRKTIARIAETAELRPDLPVLEAGPGEGLLTRELADRARQVTSYEIDP 93
Query: 75 RIIEILRNEYSWN-NVEIIQGDAVKVEWPSFN-KVVSNIPYQISSPFTFKLLKM-EFERA 131
R+ + LR + S + N+E++ D + E P V IPY I+S L+ E A
Sbjct: 94 RLAKSLREKLSGHPNIEVVNADFLTAEPPPEPFAFVGAIPYGITSAIVDWCLEAPTIETA 153
Query: 132 VVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITP 191
++ QLEFA + T G +SRL++MT L + E V KVDSA++ +
Sbjct: 154 TMVTQLEFARKRTGDYG--RWSRLTVMTWPLFEWEFV-----------EKVDSAIMRLRR 200
Query: 192 KKD------RIELNESLVKALF 207
+ + +E ES+V+ F
Sbjct: 201 RAEPLLEGAALERYESMVELCF 222
>prf||1112175A transferase,N-Me [Saccharopolyspora erythraea]
Length = 370
Score = 81.5 bits (198), Expect = 9e-15
Identities = 68/202 (33%), Positives = 103/202 (50%), Gaps = 22/202 (10%)
Query: 15 RPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDR 74
R + GQ+FL + I + E AE+ VLE GPG G LT EL+ RA++V + E+D
Sbjct: 34 RNRRQFGQNFLRDRKTIARIAETAELRPDLPVLEAGPGEGLLTRELADRARQVTSYEIDP 93
Query: 75 RIIEILRNEYSWN-NVEIIQGDAVKVEWPSFN-KVVSNIPYQISSPFTFKLLKM-EFERA 131
R+ + LR + S + N+E++ D + E P V IPY I+S L+ E A
Sbjct: 94 RLAKSLREKLSGHPNIEVVNADFLTAEPPPEPFAFVGAIPYGITSAIVDWCLEAPTIETA 153
Query: 132 VVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITP 191
++ QLEFA + T G +SRL++MT L + E V KVDSA++ +
Sbjct: 154 TMVTQLEFARKRTGDYG--RWSRLTVMTWPLFEWEFV-----------EKVDSAIMRLRR 200
Query: 192 KKD------RIELNESLVKALF 207
+ + +E ES+V+ F
Sbjct: 201 RAEPLLEGAALERYESMVELCF 222
>sp|P13079|CARB_STRTH RRNA METHYLTRANSFERASE (CARBOMYCIN-RESISTANCE PROTEIN)
>gi|99022|pir||A26512 carB protein - Streptomyces sp
>gi|153200|gb|AAC32026.1| (M16503) carbomycin resistance
protein [Streptomyces thermotolerans]
Length = 299
Score = 81.1 bits (197), Expect = 1e-14
Identities = 53/170 (31%), Positives = 91/170 (53%), Gaps = 5/170 (2%)
Query: 21 GQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEIL 80
GQ+FL+ ++ + + + A+ +VVLEVG G G +T EL++ ++V E+DR + L
Sbjct: 53 GQNFLVDRETVQRFVRFADPDPGEVVLEVGAGNGAITRELARLCRRVVAYEIDRHFADRL 112
Query: 81 RNEYSWN-NVEIIQGDAVKVEWPSFN-KVVSNIPYQISSPFT-FKLLKMEFERAVVMYQL 137
R + + +E++ GD +K P VV NIP+ ++ + L ++ QL
Sbjct: 113 REATAEDPRIEVVAGDFLKTSQPKVPFSVVGNIPFGNTADIVDWCLNARRLRTTTLVTQL 172
Query: 138 EFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALV 187
E+A + T G R +SRL++ T + + RI + F P P VDSA++
Sbjct: 173 EYARKRTG--GYRRWSRLTVATWPEVEWRMGERISRRWFRPVPAVDSAVL 220
>sp|Q00014|ERMG_LACRE RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERYTHROMYCIN RESISTANCE PROTEIN)
>gi|148728|gb|AAA98096.1| (M64090) methylase [Plasmid
pGT633]
Length = 244
Score = 81.1 bits (197), Expect = 1e-14
Identities = 51/190 (26%), Positives = 103/190 (53%), Gaps = 9/190 (4%)
Query: 15 RPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDR 74
+ N + Q+F+ K I++ + ++ +D ++E+G G G + EL+KR V IE+D
Sbjct: 3 KKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHFSFELAKRCNYVTAIEIDP 62
Query: 75 RIIEILRNE-YSWNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLL-KMEFER 130
++ I +N+ + N ++I D ++ ++P K+ NIPY IS+ K++ +
Sbjct: 63 KLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIFGNIPYNISTDIIRKIVFESTATE 122
Query: 131 AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ ++ + FA R+ + N S L+L D+ I+ +I + F+PKP+V+S+L+++
Sbjct: 123 SYLIVEYGFAKRLL----NTNRS-LALFLMTEVDISILSKIPREYFHPKPRVNSSLIVLK 177
Query: 191 PKKDRIELNE 200
+I L +
Sbjct: 178 RHPSKISLKD 187
>sp|P13957|ERM3_STAAU RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERYTHROMYCIN RESISTANCE PROTEIN)
>gi|80332|pir||B29827
macrolide/lincosamide/streptogramin B resistance
methylase - Staphylococcus aureus plasmid pE5
>gi|153065|gb|AAA98228.1| (M17990) ermC gene product
[Plasmid pE5] >gi|8927568|gb|AAF82122.1|AF276982_3
(AF276982) erythromycin resistance [Integrative vector
pNLE1]
Length = 244
Score = 80.8 bits (196), Expect = 2e-14
Identities = 59/228 (25%), Positives = 112/228 (48%), Gaps = 16/228 (7%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+F+ K IDK + +++ D + E+G G G T EL +R V IE+D ++
Sbjct: 5 NIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFTLELVQRCNFVTAIEIDHKL 64
Query: 77 IEILRNE-YSWNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEF-ERAV 132
+ N+ +N +++ D ++ ++P K+ NIPY IS+ K++ +
Sbjct: 65 CKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNIPYNISTDIIRKIVFDSIADEIY 124
Query: 133 VMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPK 192
++ + FA R+ L+L A D+ I+ + + F+PKP+V+S+L+ + K
Sbjct: 125 LIVEYGFAKRLL-----NTKRSLALFLMAEVDISILSMVPREYFHPKPRVNSSLIRLNRK 179
Query: 193 KDRI------ELNESLVKALFQHRRKVVSK-ALRESAHMLGIKDVKTV 233
K RI + N ++K + + +K+ +K S GI D+ +
Sbjct: 180 KSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGIDDLNNI 227
>gi|9507371 macrolide-lincosamide-streptogramin B-resistance protein [Plasmid
pNE131] >gi|127201|sp|P06572|ERMM_STAEP RRNA ADENINE
N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERYTHROMYCIN RESISTANCE PROTEIN)
>gi|79921|pir||A24497 erythromycin resistance protein -
Staphylococcus epidermidis plasmid pNE131
>gi|150831|gb|AAA98296.1| (M12730)
macrolide-lincosamide-streptogramin B-resistance protein
[Plasmid pNE131]
Length = 244
Score = 80.4 bits (195), Expect = 2e-14
Identities = 59/228 (25%), Positives = 111/228 (47%), Gaps = 16/228 (7%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+F+ K IDK + +++ D + E+G G G T EL +R V IE+D ++
Sbjct: 5 NIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFTLELVQRCNFVTAIEIDHKL 64
Query: 77 IEILRNE-YSWNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEF-ERAV 132
+ N+ +N +++ D ++ ++P K+ NIPY IS+ K++ +
Sbjct: 65 CKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNIPYNISTDIIRKIVFDSIADEIY 124
Query: 133 VMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPK 192
++ + FA R+ +L A D+ I+ + + F+PKPKV+S+L+ + K
Sbjct: 125 LIVEYGFAKRLL-----NTKRSFALFLMAEVDISILSMVPREYFHPKPKVNSSLIRLNRK 179
Query: 193 KDRI------ELNESLVKALFQHRRKVVSK-ALRESAHMLGIKDVKTV 233
K RI + N ++K + + +K+ +K S GI D+ +
Sbjct: 180 KSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGIDDLNNI 227
>gb|AAB60941.1| (AF002716) erythromycin resistance methylase [Streptococcus
pyogenes]
Length = 243
Score = 80.4 bits (195), Expect = 2e-14
Identities = 56/216 (25%), Positives = 104/216 (47%), Gaps = 13/216 (6%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+F+ K + + ++ ++K D ++E+G G G T EL + +++V IE+D +
Sbjct: 5 NPKNTQNFITSKKHVKEILKYTNINKQDKIIEIGSGKGHFTKELVEMSQRVNAIEIDEGL 64
Query: 77 IEILRNEYS-WNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N+++I D +K +P K+ NIPY IS+ + K+ F+ +
Sbjct: 65 CHATKKAVEPFQNIKVIHEDILKFSFPKNTDYKIFGNIPYNISTDI---VKKIAFD-SQA 120
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPKK 193
Y R AK L L+ D++I+ ++ + F+PKP VDS L+++ K
Sbjct: 121 KYSYLIVERGFAKRLQNTQRALGLLLMVEMDIKILKKVPRAYFHPKPNVDSVLIVLERHK 180
Query: 194 DRIELNESLVKALFQHRRKVVSKALRESAHMLGIKD 229
I +K ++ R V K + H+L K+
Sbjct: 181 PFI------LKKDYKKYRFFVYKWVNREYHVLFTKN 210
>gb|AAC45552.1| (U82607) rRNA N-6-methyltransferase [Staphylococcus chromogenes]
Length = 244
Score = 79.2 bits (192), Expect = 5e-14
Identities = 59/228 (25%), Positives = 112/228 (48%), Gaps = 16/228 (7%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+F+ K IDK + +++ D + E+G G G T EL +R V IE+D ++
Sbjct: 5 NIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFTLELVQRCNFVTAIEIDHKL 64
Query: 77 IEILRNE-YSWNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEF-ERAV 132
+ N+ +N +++ D ++ ++P K+ NI Y IS+ K++ +
Sbjct: 65 CKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNIRYNISTDIIRKIVFDSIADEIY 124
Query: 133 VMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPK 192
++ + FA R+ L+L+ A D+ I+ + + F+PKPKV+S+L+ + K
Sbjct: 125 LIVEYGFAKRLL-----NTKRSLALLLMAEVDISILSMVPREYFHPKPKVNSSLIRLNRK 179
Query: 193 KDRI------ELNESLVKALFQHRRKVVSK-ALRESAHMLGIKDVKTV 233
K RI + N ++K + + +K+ +K S GI D+ +
Sbjct: 180 KSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGIDDLNNI 227
>sp|P06699|ERM1_STAAU RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERYTHROMYCIN RESISTANCE PROTEIN)
>gi|79878|pir||A25101 erythromycin resistance protein
ermA - Staphylococcus aureus transposon Tn554
>gi|43731|emb|CAA26964.1| (X03216) S-adenosyl-methionine
dependent methylase [Staphylococcus aureus]
>gi|5360835|dbj|BAA82205.1| (D86934) rRNA adenine
N-6-methyltransferase [Staphylococcus aureus]
>gi|224805|prf||1202257A gene ermA [Staphylococcus
aureus]
Length = 243
Score = 78.4 bits (190), Expect = 8e-14
Identities = 49/178 (27%), Positives = 95/178 (52%), Gaps = 11/178 (6%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+F+ K + + + +SK D V+E+G G G T EL K ++ V IE+D +
Sbjct: 5 NPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHFTKELVKMSRSVTAIEIDGGL 64
Query: 77 IEILRNEYS-WNNVEIIQGDAVKVEWPSF--NKVVSNIPYQISSPFTFKL-LKMEFERAV 132
++ + + N+++IQ D +K +P K+ NIPY IS+ ++ + + + +
Sbjct: 65 CQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNIPYNISTDIVKRITFESQAKYSY 124
Query: 133 VMYQLEFALRMTAKPGDRNYSR-LSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLI 189
++ + FA R+ +N R L L+ D++++ ++ F+PKP VDS L+++
Sbjct: 125 LIVEKGFAKRL------QNLQRALGLLLMVEMDIKMLKKVPPLYFHPKPSVDSVLIVL 176
>gb|AAC33146.1| (AF019140) rRNA N-6-methyltransferase [Staphylococcus simulans]
Length = 244
Score = 78.4 bits (190), Expect = 8e-14
Identities = 59/228 (25%), Positives = 111/228 (47%), Gaps = 16/228 (7%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+F+ K IDK + +++ D + E+G G G T EL +R V IE+D ++
Sbjct: 5 NIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFTLELVQRCNFVTAIEIDHKL 64
Query: 77 IEILRNE-YSWNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEF-ERAV 132
+ N+ +N +++ D ++ ++P K+ NI Y IS+ K++ +
Sbjct: 65 CKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNIRYNISTDIIRKIVFDSIADEIY 124
Query: 133 VMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPK 192
++ + FA R+ L+L A D+ I+ + + F+PKPKV+S+L+ + K
Sbjct: 125 LIVEYGFAKRLL-----NTKRSLALFLMAEVDISILSMVPREYFHPKPKVNSSLIRLNRK 179
Query: 193 KDRI------ELNESLVKALFQHRRKVVSK-ALRESAHMLGIKDVKTV 233
K RI + N ++K + + +K+ +K S GI D+ +
Sbjct: 180 KSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGIDDLNNI 227
>gb|AAA26492.1| (M11200) N-6-amino adenine-N-methyl transferase [Saccharopolyspora
erythraea]
Length = 370
Score = 78.0 bits (189), Expect = 1e-13
Identities = 67/202 (33%), Positives = 102/202 (50%), Gaps = 22/202 (10%)
Query: 15 RPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDR 74
R + GQ+FL + I + E AE+ VLE GP G LT EL+ RA++V + E+D
Sbjct: 34 RNRRQFGQNFLRDRKTIARIAETAELRPDLPVLEAGPVEGLLTRELADRARQVTSYEIDP 93
Query: 75 RIIEILRNEYSWN-NVEIIQGDAVKVEWPSFN-KVVSNIPYQISSPFTFKLLKM-EFERA 131
R+ + LR + S + N+E++ D + E P V IPY I+S L+ E A
Sbjct: 94 RLAKSLREKLSGHPNIEVVNADFLTAEPPPEPFAFVGAIPYGITSAIVDWCLEAPTIETA 153
Query: 132 VVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITP 191
++ QLEFA + T G +SRL++MT L + E V KVDSA++ +
Sbjct: 154 TMVTQLEFARKRTGDYG--RWSRLTVMTWPLFEWEFV-----------EKVDSAIMRLRR 200
Query: 192 KKD------RIELNESLVKALF 207
+ + +E ES+V+ F
Sbjct: 201 RAEPLLEGAALERYESMVELCF 222
>emb|CAA70206.1| (Y09001) adenine methylase [Staphylococcus hominis]
Length = 244
Score = 78.0 bits (189), Expect = 1e-13
Identities = 61/229 (26%), Positives = 112/229 (48%), Gaps = 18/229 (7%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q F+ K IDK + +++ D + E+G G G T EL +R V IE+D ++
Sbjct: 5 NIKHSQKFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGDFTLELVQRCNFVTAIEIDHKL 64
Query: 77 IEILRNEY-SWNNVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEF-ERAV 132
+ + +N +++ D ++ ++P K+ NIPY IS+ K++ +
Sbjct: 65 CKTTEKKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNIPYNISTDIIRKIVFDSIADEIY 124
Query: 133 VMYQLEFALRMTAKPGDRNYSR-LSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITP 191
++ + FA R+ N R L+L A D+ I+ + + F+PKPKV+S+L+ +
Sbjct: 125 LIVEYGFAKRLL------NTKRSLALFLMAEVDISILSMVPREYFHPKPKVNSSLIRLNR 178
Query: 192 KKDRI------ELNESLVKALFQHRRKVVSK-ALRESAHMLGIKDVKTV 233
KK RI + N ++K + + +K+ +K S GI D+ +
Sbjct: 179 KKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGIDDLNNI 227
>pir||S68607 protein Erm2 - Streptococcus pyogenes plasmid pBT233
>gi|456370|emb|CAA45935.1| (X64695) erm2 [Streptococcus
pyogenes] >gi|1532095|gb|AAC44378.1| (U35228) adenine
methylase [Staphylococcus lentus]
>gi|1835782|gb|AAC71783.1| (U86375) adenine methylase
[Enterococcus faecalis] >gi|6273679|emb|CAA73921.1|
(Y13600) rRNA methylase [Staphylococcus aureus]
>gi|739954|prf||2004266G erm2 protein [Streptococcus
pyogenes]
Length = 245
Score = 77.3 bits (187), Expect = 2e-13
Identities = 48/177 (27%), Positives = 93/177 (52%), Gaps = 7/177 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 4 NIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 63
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V NIPY +S+ K++ +
Sbjct: 64 FNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESHASDIY 123
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKPKV+S L+ +T
Sbjct: 124 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLT 176
>sp|P20173|ERM2_ENTFA RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) >gi|79948|pir||B25028 erythromycin resistance
protein, ORF2 - Enterococcus faecalis
>gi|154959|gb|AAA27452.1| (M11180) rRNA methylase
[Transposon Tn917] >gi|1185588|gb|AAB40318.1| (U46200)
erythromycin resistance; erythromycin resistance
determinant [Cloning vector pDG1663]
>gi|1185596|gb|AAB40325.1| (U46201) erythromycin
resistance; erythromycin resistance determinant
[Cloning vector pDG1664] >gi|1277133|gb|AAC53690.1|
(U50977) erythromycin-resistance protein [Cloning vector
pFW15]
Length = 245
Score = 76.9 bits (186), Expect = 2e-13
Identities = 48/177 (27%), Positives = 93/177 (52%), Gaps = 7/177 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 4 NIKYSQNFLTNEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 63
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V NIPY +S+ K++ +
Sbjct: 64 FNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESHASDIY 123
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKPKV+S L+ +T
Sbjct: 124 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLT 176
>pir||S45087 probable MSL methylase erm2 - Streptococcus pyogenes plasmid pDB101
and pBT233 >gi|496516|emb|CAA47093.1| (X66468) erm2
[Streptococcus pyogenes] >gi|3127117|gb|AAC16049.1|
(AF061336) erythromycin resistance determinant [Tn917
delivery vector pAJ005]
Length = 253
Score = 76.9 bits (186), Expect = 2e-13
Identities = 48/177 (27%), Positives = 93/177 (52%), Gaps = 7/177 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 12 NIKYSQNFLTNEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 71
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V NIPY +S+ K++ +
Sbjct: 72 FNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESHASDIY 131
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKPKV+S L+ +T
Sbjct: 132 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLT 184
>gi|10956213 MSL methylase [Streptococcus agalactiae] >gi|1363996|pir||S57623
adenine methylase (EC 2.1.1.-) - Enterococcus sp.
plasmid (strain RE39) >gi|2117692|pir||I39481 MSL
methylase - Streptococcus agalactiae
>gi|2981999|pdb|1YUB| Solution Structure Of An Rrna
Methyltransferase (Ermam) That Confers
Macrolide-Lincosamide-Streptogramin Antibiotic
Resistance, Nmr, Minimized Average Structure
>gi|511078|emb|CAA50902.1| (X72021) MSL methylase
[Streptococcus agalactiae] >gi|886483|emb|CAA58028.1|
(X82819) adenine methylase [Enterococcus sp.]
Length = 245
Score = 76.5 bits (185), Expect = 3e-13
Identities = 48/177 (27%), Positives = 93/177 (52%), Gaps = 7/177 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 4 NIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 63
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V NIPY +S+ K++ +
Sbjct: 64 FNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESRASDIY 123
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKPKV+S L+ +T
Sbjct: 124 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLT 176
>gb|AAC45607.1| (U48430) rRNA methylase [Lactobacillus fermentum]
Length = 250
Score = 76.5 bits (185), Expect = 3e-13
Identities = 48/177 (27%), Positives = 93/177 (52%), Gaps = 7/177 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 4 NIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 63
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V NIPY +S+ K++ +
Sbjct: 64 FNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESRASDIY 123
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKPKV+S L+ +T
Sbjct: 124 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLT 176
>gb|AAB86539.1| (AF030064) erythromycin resistance determinant [Lactobacillus
reuteri]
Length = 248
Score = 76.5 bits (185), Expect = 3e-13
Identities = 48/177 (27%), Positives = 93/177 (52%), Gaps = 7/177 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 4 NIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 63
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V NIPY +S+ K++ +
Sbjct: 64 FNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTKIIKKVVFESRASDIY 123
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKPKV+S L+ +T
Sbjct: 124 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLT 176
>gb|AAC31204.1| (AF080450) rRNA methylase [Lactobacillus reuteri]
Length = 250
Score = 76.5 bits (185), Expect = 3e-13
Identities = 48/177 (27%), Positives = 93/177 (52%), Gaps = 7/177 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 4 NIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 63
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V NIPY +S+ K++ +
Sbjct: 64 FNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESRASDIY 123
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKPKV+S L+ +T
Sbjct: 124 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLT 176
>pir||B27739 erythromycin resistance protein ermBC - Escherichia coli
Length = 245
Score = 76.1 bits (184), Expect = 4e-13
Identities = 47/177 (26%), Positives = 93/177 (51%), Gaps = 7/177 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 4 NIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 63
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V NIPY +S+ K++ +
Sbjct: 64 FNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESHASDIY 123
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKP+V+S L+ +T
Sbjct: 124 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIQQLLKLPAECFHPKPRVNSVLIKLT 176
>sp|P10738|ERMB_ECOLI RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERYTHROMYCIN RESISTANCE PROTEIN)
>gi|150715|gb|AAA25634.1| (M19270) 23S rRNA methylase
[Plasmid pIP1527]
Length = 245
Score = 75.7 bits (183), Expect = 5e-13
Identities = 47/177 (26%), Positives = 93/177 (51%), Gaps = 7/177 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 4 NIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 63
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V NIPY +S+ K++ +
Sbjct: 64 FNLSSEKLKSNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESHASDIY 123
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKP+V+S L+ +T
Sbjct: 124 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIQQLLKLPAECFHPKPRVNSVLIKLT 176
>sp|P06573|ERM_STRSA RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) >gi|150491|gb|AAA25527.1| (K00551) adenine
methylase [Plasmid pAM77]
Length = 245
Score = 75.7 bits (183), Expect = 5e-13
Identities = 47/180 (26%), Positives = 95/180 (52%), Gaps = 7/180 (3%)
Query: 14 IRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELD 73
++ N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD
Sbjct: 1 MKKNIKYSQNFLTNEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELD 60
Query: 74 RRIIEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFER 130
+ + + N V +I D ++ ++P+ K+V +IPY +S+ K++
Sbjct: 61 SHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIPYHLSTQIIKKVVFESHAS 120
Query: 131 AVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ + E + T + L L+ ++ ++++ F+PKPKV+S L+ +T
Sbjct: 121 DIYLIVEEGFYKRTLD----IHRSLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLT 176
>gb|AAD37840.1|AF109075_2 (AF109075) rRNA methyltransferase ErmBZ1 [Clostridium difficile]
>gi|5019748|gb|AAD37843.1|AF109075_5 (AF109075) rRNA
methyltransferase ErmBZ2 [Clostridium difficile]
Length = 245
Score = 75.7 bits (183), Expect = 5e-13
Identities = 48/177 (27%), Positives = 94/177 (52%), Gaps = 7/177 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 4 NIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 63
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V +IPY +S+ K++ +
Sbjct: 64 FNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYHLSTQIIKKVVFESRASDIY 123
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKPKV+SAL+ +T
Sbjct: 124 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIKQLLKLPAECFHPKPKVNSALIKLT 176
>sp|P12038|ERM1_ENTFA RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) >gi|98622|pir||S16033 rRNA methylase ermBP -
Clostridium perfringens >gi|40558|emb|CAA41221.1|
(X58285) ribosomal RNA methylase [Clostridium
perfringens] >gi|47020|emb|CAA68299.1| (Y00116) adenine
methylase (ORF2)(AA 1-245) [Enterococcus faecalis]
>gi|144783|gb|AAC36955.1| (L02938) RNA methylase
[Shuttle vector pJIR751] >gi|208134|gb|AAA73116.1|
(M77169) RNA methylase [unidentified cloning vector]
>gi|220962|dbj|BAA14385.1| (D90381) MLS resistance
protein [synthetic construct] >gi|841202|gb|AAC43480.1|
(U18931) ErmBP [Clostridium perfringens]
>gi|2745828|gb|AAB94755.1| (AF039139) adenine methylase
[Cloning vector pIL252] >gi|2773394|gb|AAB96789.1|
(AF041239) adenine methylase [Cloning vector pIL253]
>gi|3132863|gb|AAC16408.1| (U90555) erythromycin
resistance gene [Shuttle vector pJIR1457]
>gi|3138989|gb|AAD11463.1| (AF060240) rRNA adenine
N-6-methyltransferase [synthetic construct]
>gi|3320598|gb|AAC83650.1| (AF072806) adenine methylase
[Integrational vector pMUTIN2]
>gi|5459333|emb|CAB50726.1| (AJ243540)
adenin-N6-methylase [Expression vector pERM-ex1]
>gi|5459338|emb|CAB50711.1| (AJ243541)
adenin-N6-methylase [Cloning vector pAMY-em1]
>gi|6979875|gb|AAF34637.1| (AF219231) ErmAM [Shuttle
vector pHS17] >gi|9963916|gb|AAG09762.1| (AF239773)
erythromycin resistance methylase [Staphylococcus
intermedius]
Length = 245
Score = 75.3 bits (182), Expect = 7e-13
Identities = 47/177 (26%), Positives = 93/177 (51%), Gaps = 7/177 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 4 NIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 63
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V +IPY +S+ K++ +
Sbjct: 64 FNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYHLSTQIIKKVVFESHASDIY 123
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKPKV+S L+ +T
Sbjct: 124 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLT 176
>pir||A27507 DNA adenine methylase (EC 2.1.1.-), ORF2 and ORF3 - Enterococcus
faecalis plasmid pAMbeta1 (fragments)
Length = 286
Score = 75.3 bits (182), Expect = 7e-13
Identities = 47/177 (26%), Positives = 93/177 (51%), Gaps = 7/177 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 4 NIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 63
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V +IPY +S+ K++ +
Sbjct: 64 FNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYHLSTQIIKKVVFESHASDIY 123
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKPKV+S L+ +T
Sbjct: 124 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLT 176
>sp|P21236|ERM_STRPN RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERMAM) >gi|98052|pir||S12727 rRNA
(adenine-N6-)-methyltransferase (EC 2.1.1.48) -
Streptococcus pneumoniae transposon Tn1545
Length = 245
Score = 75.3 bits (182), Expect = 7e-13
Identities = 47/177 (26%), Positives = 93/177 (51%), Gaps = 7/177 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 4 NIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 63
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V +IPY +S+ K++ +
Sbjct: 64 FNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIPYHLSTQIIKKVVFESHASDIY 123
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKPKV+S L+ +T
Sbjct: 124 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLT 176
>gb|AAG09761.1| (AF239772) erythromycin resistance methylase [Staphylococcus
intermedius]
Length = 245
Score = 75.3 bits (182), Expect = 7e-13
Identities = 47/177 (26%), Positives = 93/177 (51%), Gaps = 7/177 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 4 NIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 63
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V +IPY +S+ K++ +
Sbjct: 64 FNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIPYHLSTQIIKKVVFESHASDIY 123
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKPKV+S L+ +T
Sbjct: 124 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLT 176
>pir||S49316 adenine methylase (EC 2.1.1.-) - Enterococcus hirae
Length = 245
Score = 74.9 bits (181), Expect = 9e-13
Identities = 50/180 (27%), Positives = 96/180 (52%), Gaps = 13/180 (7%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 4 NIKYSQNFLTNEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 63
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V +IPY +S+ + K+ FE
Sbjct: 64 FNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYHLSTQI---IKKVVFE---- 116
Query: 134 MYQLEFALRMTAKPGDRN---YSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ + L + K R + L L+ ++ ++++ F+PKPKV+S L+ +T
Sbjct: 117 SHASDIYLIVEKKKKKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLT 176
>emb|CAA57314.1| (X81655) adenine methylase [Enterococcus hirae]
Length = 245
Score = 74.9 bits (181), Expect = 9e-13
Identities = 47/177 (26%), Positives = 93/177 (51%), Gaps = 7/177 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 4 NIKYSQNFLTNEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 63
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V +IPY +S+ K++ +
Sbjct: 64 FNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYHLSTQIIKKVVFESHASDIY 123
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKPKV+S L+ +T
Sbjct: 124 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLT 176
>emb|CAA08815.1| (AJ009761) putative dimethyladenosine transferase [Homo sapiens]
Length = 157
Score = 74.9 bits (181), Expect = 9e-13
Identities = 40/88 (45%), Positives = 56/88 (63%), Gaps = 4/88 (4%)
Query: 139 FALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLITPKKDRIEL 198
FALR+ AKPGD+ Y RLS+ TQ LA V+ +M++GK F P PKV+S++V I PK +
Sbjct: 3 FALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPI 62
Query: 199 N----ESLVKALFQHRRKVVSKALRESA 222
N + LV+ F + K +S A + SA
Sbjct: 63 NFQEWDGLVRITFVRKNKTLSAAFKSSA 90
>gb|AAF64431.1|AF229200_4 (AF229200) erythromycin resistance methylase [Enterococcus faecium]
>gi|9246953|gb|AAF86219.1|AF242872_2 (AF242872) ErmB
[Enterococcus faecium]
Length = 248
Score = 74.5 bits (180), Expect = 1e-12
Identities = 47/177 (26%), Positives = 93/177 (51%), Gaps = 7/177 (3%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V +IELD +
Sbjct: 4 NIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHL 63
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V +IPY +S+ K++ +
Sbjct: 64 FNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYNLSTQIIKKVVFESRASDIY 123
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKPKV+S L+ +T
Sbjct: 124 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLT 176
>sp|P09891|ERMA_ARTS3 RRNA ADENINE N-6-METHYLTRANSFERASE
(MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN B RESISTANCE
PROTEIN) (ERYTHROMYCIN RESISTANCE PROTEIN)
>gi|80586|pir||A24026 erythromycin resistance protein -
Arthrobacter sp >gi|142204|gb|AAA22075.1| (M11276)
erythromycin resistance (ermA) protein [Arthrobacter
sp.]
Length = 340
Score = 74.1 bits (179), Expect = 2e-12
Identities = 55/178 (30%), Positives = 92/178 (50%), Gaps = 7/178 (3%)
Query: 15 RPNDR--IGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIEL 72
R DR +GQ+FL I + + A+V +V+E GPG G LT EL++RA +V T EL
Sbjct: 28 RDRDRRVLGQNFLRDPATIRRIADAADVDPDGLVVEAGPGEGLLTRELARRAGRVRTYEL 87
Query: 73 DRRIIEILRNEYSW-NNVEIIQGDAVKVEWPSFN-KVVSNIPYQISSPFT-FKLLKMEFE 129
D+R+ L + + ++E++ D + P + V IPY I+S + L
Sbjct: 88 DQRLARRLSTDLAQETSIEVVHADFLTAPHPEEPFQFVGAIPYGITSAIVDWCLTAPTLT 147
Query: 130 RAVVMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALV 187
A ++ Q EFA + T G ++ L++ T + + V ++ + F P P+V SA++
Sbjct: 148 SATLVTQQEFARKRTGDYG--RWTALTVTTWPTFEWQYVAKVDRTLFTPVPRVHSAIM 203
>gb|AAA73396.1| (U00453) adenine methylase [Streptococcus agalactiae]
Length = 244
Score = 74.1 bits (179), Expect = 2e-12
Identities = 49/177 (27%), Positives = 93/177 (51%), Gaps = 8/177 (4%)
Query: 17 NDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRI 76
N + Q+FL + V+++ I+ + ++D V E+G G G LT +L+K +K+V TIELD +
Sbjct: 4 NIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQV-TIELDSHL 62
Query: 77 IEILRNEYSWN-NVEIIQGDAVKVEWPSFN--KVVSNIPYQISSPFTFKLLKMEFERAVV 133
+ + N V +I D ++ ++P+ K+V NIPY +S+ K++ +
Sbjct: 63 FNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESRASDIY 122
Query: 134 MYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLIT 190
+ E + T + L L+ ++ ++++ F+PKPKV+S L+ +T
Sbjct: 123 LIVEEGFYKRTLD----IHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLT 175
>pir||E71821 dimethyladenosine transferase - Helicobacter pylori (strain J99)
>gi|4155935|gb|AAD06902.1| (AE001555) DIMETHYLADENOSINE
TRANSFERASE [Helicobacter pylori J99]
Length = 271
Score = 73.7 bits (178), Expect = 2e-12
Identities = 67/229 (29%), Positives = 111/229 (48%), Gaps = 33/229 (14%)
Query: 20 IGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIE- 78
+GQHFL+ + +D+ + S ++E+G GLG LT +L R + T E+D + E
Sbjct: 8 LGQHFLMDESFLDRIVNALPPLNSLRLIEIGVGLGDLTLKLLDR-YPLKTYEIDSNLCEK 66
Query: 79 ---ILRNEYSWNNVEIIQGDA--VKVEWPSFNKVVSNIPYQISSPFTFKLLK-MEFERAV 132
LR E +E+++ DA +K E P F ++SN+PY I++ LK + +
Sbjct: 67 MRARLRAEKKPFQLELVEKDALFLKEEEPYF--LISNLPYYIATRLVLNALKDPKCRGLL 124
Query: 133 VMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSAL--VLIT 190
VM Q E AL+ AK + + LS++ + + ++ + AF P PKV S++ V+
Sbjct: 125 VMTQKEVALKFCAKD---SQNALSVLAHTIGNATLLFDVPPSAFSPPPKVFSSVFEVIKE 181
Query: 191 PKKDR------------------IELNESLVKALFQHRRKVVSKALRES 221
P K++ E E +KA F RK +S L++S
Sbjct: 182 PLKEKALASLAQAPFFEEALQKGFETLEDFLKACFSSPRKTLSNNLKKS 230
>gb|AAF76480.1|AC020622_14 (AC020622) Identical to dimethyladenosine transferase (PFC1) from
Arabidopsis thaliana gb|AF051326 and contains a
Ribosomal RNA Adenine Dimethylases PF|00398 domain.
This gene may be cut off
Length = 239
Score = 72.6 bits (175), Expect = 5e-12
Identities = 48/155 (30%), Positives = 78/155 (49%), Gaps = 20/155 (12%)
Query: 9 LSKYGIRPNDRIGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVF 68
L+ G P +GQH+++ D+ D+ A+V + D VLE+GPG G LT+ L V
Sbjct: 64 LNSRGRFPRKSLGQHYMLNSDINDQLASAADVKEGDFVLEIGPGTGSLTNVLINLGATVL 123
Query: 69 TIELDRRIIEILRNEYSWNN-VEIIQGDAVKVEWPS-----------------FNKVVSN 110
IE D +++++ ++ ++ +++Q D VK S KVVSN
Sbjct: 124 AIEKDPHMVDLVSERFAGSDKFKVLQEDFVKCHIRSHMLSILETRRLSHPDSALAKVVSN 183
Query: 111 IPYQISSPFTFKLLKME--FERAVVMYQLEFALRM 143
+P+ IS+ LL M F + V++ Q E ALR+
Sbjct: 184 LPFNISTDVVKLLLPMGDIFSKVVLLLQDEAALRL 218
>pir||G64698 16S rRNA (adenosine-N6,N6-)-dimethyltransferase (EC 2.1.1.-) -
Helicobacter pylori (strain 26695)
>gi|2314603|gb|AAD08470.1| (AE000643) 16S rRNA
(adenosine-N6,N6-)-dimethyltransferase (ksgA)
[Helicobacter pylori 26695]
Length = 271
Score = 71.4 bits (172), Expect = 1e-11
Identities = 63/229 (27%), Positives = 110/229 (47%), Gaps = 33/229 (14%)
Query: 20 IGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEI 79
+GQHFL + +D+ + ++E+G GLG LT +L R + T E+D + E
Sbjct: 8 LGQHFLTDESFLDRIVNALPPLNPLKLVEIGVGLGDLTLKLLDR-YPLKTYEIDSHLCEK 66
Query: 80 LRNEYSWN----NVEIIQGDA--VKVEWPSFNKVVSNIPYQISSPFTFKLLK-MEFERAV 132
+R++ +E+++ DA +K E P F ++SN+PY I++ K + +
Sbjct: 67 MRSKLKAQKKPFKLELVEKDALFLKEEEPYF--LISNLPYYIATRLVLNAFKDPKCRGLL 124
Query: 133 VMYQLEFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSAL--VLIT 190
VM Q E AL+ AK + + LS++ + + ++ + AF P PKV S++ V+
Sbjct: 125 VMTQKEVALKFCAKD---SQNALSVLAHTIGNATLLFDVPPSAFSPPPKVFSSVFEVIKE 181
Query: 191 PKKDR------------------IELNESLVKALFQHRRKVVSKALRES 221
P K++ E+ E +KA F RK +S L++S
Sbjct: 182 PLKEKALASLAQAPFFEEALQKGFEMLEDFLKACFSSPRKTLSNNLKKS 230
>sp|P43433|MYRB_MICGR MYCINAMICIN-RESISTANCE PROTEIN MYRB >gi|286054|dbj|BAA03402.1|
(D14532) myrB [Micromonospora griseorubida]
Length = 311
Score = 70.6 bits (170), Expect = 2e-11
Identities = 49/172 (28%), Positives = 91/172 (52%), Gaps = 6/172 (3%)
Query: 20 IGQHFLIVKDVIDKAIEVAEVSKSDVVLEVGPGLGFLTDELSKRAKKVFTIELDRRIIEI 79
+GQ+FL+ + V + EV + + VLE+G G G +T L V +ELD R +
Sbjct: 24 LGQNFLVDRGVCTRIAEVVSSTTAHPVLELGAGDGAITRALVAANLPVTALELDPRRVRR 83
Query: 80 LRNEYSWNNVEIIQGDAVKVEW-PSFNKVVSNIPYQISSPFTFKLLKMEF-ERAVVMYQL 137
L+ ++ + V ++ GD ++ ++ P + VVS +P+ I++P +L+ F AV++ Q
Sbjct: 84 LQRTFA-DGVTVVHGDMLRYDFGPYPHHVVSTVPFSITTPLLRRLIGQRFWHTAVLLVQW 142
Query: 138 EFALRMTAKPGDRNYSRLSLMTQALADVEIVMRIGKGAFYPKPKVDSALVLI 189
E A + + G + L+ + + +V R+ K +F P P VD +++I
Sbjct: 143 EVARK---RAGVGGTTMLTAASWPWYEFTLVERVPKTSFDPVPSVDGGILVI 191
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.321 0.138 0.385
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89400953
Number of Sequences: 2977
Number of extensions: 3296483
Number of successful extensions: 9964
Number of sequences better than 1.0e-10: 109
Number of HSP's better than 0.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 95
Number of HSP's that attempted gapping in prelim test: 9755
Number of HSP's gapped (non-prelim): 114
length of query: 269
length of database: 189,106,746
effective HSP length: 57
effective length of query: 212
effective length of database: 154,992,987
effective search space: 32858513244
effective search space used: 32858513244
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 164 (68.3 bits)