BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB0283 (pimT-like) DE:PROTEIN-L-ISOASPARTATE METHYLTRANSFERASE HOMOLOG (253 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||A75159 protein-l-isoaspartate methyltransferase homolog PAB... 516 e-146 pir||E71198 hypothetical protein PH1858 - Pyrococcus horikoshii ... 460 e-128 pir||D69055 protein-L-isoaspartate methyltransferase homolog - M... 198 6e-50 sp|Q57598|Y134_METJA HYPOTHETICAL PROTEIN MJ0134 >gi|2127995|pir... 186 2e-46 pir||G72340 conserved hypothetical protein - Thermotoga maritima... 172 4e-42 emb|CAB59504.1| (AL132648) hypothetical protein SCI41.34c [Strep... 164 1e-39 gb|AAC68688.1| (AF088800) unknown [Rhodococcus erythropolis] 160 2e-38 pir||A70513 hypothetical protein Rv2118c - Mycobacterium tubercu... 154 8e-37 emb|CAA22923.1| (AL035310) hypothetical protein MLCB2533.09 [Myc... 150 2e-35 pir||S72844 beta-aspartate methyltransferase pimT - Mycobacteriu... 148 5e-35 gb|AAK40752.1| Protein L-isoaspartate methyltransferase [Sulfolo... 145 7e-34 gi|11497832 L-isoaspartyl protein carboxyl methyltransferase Pim... 122 4e-27 pir||T48633 hypothetical protein T15N1.90 - Arabidopsis thaliana... 116 3e-25 gi|8923586 hypothetical protein FLJ20628 [Homo sapiens] >gi|7020... 106 2e-22 pir||F72480 probable beta-aspartate metyltransferase APE2486 - A... 104 8e-22 gb|AAF56565.1| (AE003754) CG14544 gene product [Drosophila melan... 100 3e-20 gb|AAG18973.1| (AE004998) L-isoaspartyl protein carboxyl methylt... 99 5e-20 pir||T24625 hypothetical protein W02A11.1 - Caenorhabditis elega... 90 3e-17 pir||A70328 hypothetical protein aq_311 - Aquifex aeolicus >gi|2... 89 7e-17 emb|CAC11981.1| (AL445065) conserved hypothetical protein [Therm... 79 6e-14 gb|AAC64273.1|AAC64273 (AF086832) unknown [Streptomyces coelicol... 73 3e-12 pir||T39235 probable translational repressor - fission yeast (S... 69 7e-11 >pir||A75159 protein-l-isoaspartate methyltransferase homolog PAB0283 - Pyrococcus abyssi (strain Orsay) >gi|5457862|emb|CAB49352.1| (AJ248284) PROTEIN-L-ISOASPARTATE METHYLTRANSFERASE HOMOLOG [Pyrococcus abyssi] Length = 253 Score = 516 bits (1315), Expect = e-146 Identities = 253/253 (100%), Positives = 253/253 (100%) Query: 1 MIREGDKVVLVDPRGKRYLITVSKRDFHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILR 60 MIREGDKVVLVDPRGKRYLITVSKRDFHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILR Sbjct: 1 MIREGDKVVLVDPRGKRYLITVSKRDFHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILR 60 Query: 61 PRIVDYLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGR 120 PRIVDYLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGR Sbjct: 61 PRIVDYLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGR 120 Query: 121 VVSYEIREDFAKLAWENIKWAGFDDRVTIKLKDIYEGIEEENVDHVILDLPQPERVVEHA 180 VVSYEIREDFAKLAWENIKWAGFDDRVTIKLKDIYEGIEEENVDHVILDLPQPERVVEHA Sbjct: 121 VVSYEIREDFAKLAWENIKWAGFDDRVTIKLKDIYEGIEEENVDHVILDLPQPERVVEHA 180 Query: 181 AKALKPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECMRPRTTA 240 AKALKPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECMRPRTTA Sbjct: 181 AKALKPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECMRPRTTA 240 Query: 241 LVHTGYITFARRI 253 LVHTGYITFARRI Sbjct: 241 LVHTGYITFARRI 253 >pir||E71198 hypothetical protein PH1858 - Pyrococcus horikoshii >gi|3258297|dbj|BAA30980.1| (AP000007) 253aa long hypothetical protein [Pyrococcus horikoshii] Length = 253 Score = 460 bits (1170), Expect = e-128 Identities = 212/253 (83%), Positives = 242/253 (94%) Query: 1 MIREGDKVVLVDPRGKRYLITVSKRDFHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILR 60 MIREGDK++LVDPRGKRYL+T + +DFHTDLGI+KL ++IG+NFG++IKSHKGH FKIL+ Sbjct: 1 MIREGDKIILVDPRGKRYLVTATPKDFHTDLGIIKLGDVIGKNFGDSIKSHKGHVFKILK 60 Query: 61 PRIVDYLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGR 120 PRIVDYLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGP+G+ Sbjct: 61 PRIVDYLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPQGK 120 Query: 121 VVSYEIREDFAKLAWENIKWAGFDDRVTIKLKDIYEGIEEENVDHVILDLPQPERVVEHA 180 +VSYEIREDFAKLAWENIK AGF+DRVTIKLK+IY+GI+EENVDH++LDLPQPERVVEHA Sbjct: 121 IVSYEIREDFAKLAWENIKLAGFEDRVTIKLKNIYDGIDEENVDHIVLDLPQPERVVEHA 180 Query: 181 AKALKPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECMRPRTTA 240 KALKPGGFF+AYTPC+NQVMRLH+KL EF+D FM+PRTI V+VFDQEVKKECMRP+TT Sbjct: 181 IKALKPGGFFIAYTPCANQVMRLHDKLAEFRDTFMRPRTIEVIVFDQEVKKECMRPKTTV 240 Query: 241 LVHTGYITFARRI 253 L HTGYITFAR++ Sbjct: 241 LAHTGYITFARKL 253 >pir||D69055 protein-L-isoaspartate methyltransferase homolog - Methanobacterium thermoautotrophicum (strain Delta H) >gi|2622526|gb|AAB85891.1| (AE000903) protein-L-isoaspartate methyltransferase homolog [Methanobacterium thermoautotrophicum] Length = 245 Score = 198 bits (498), Expect = 6e-50 Identities = 108/248 (43%), Positives = 156/248 (62%), Gaps = 4/248 (1%) Query: 6 DKVVLVDPRGKRYLITVSKRDFHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILRPRIVD 65 D +L+D RGK+YL+ + DF +D+GI+K E + G+ +K+H G E +L+P + D Sbjct: 2 DLRILMDERGKKYLLEEGE-DFQSDMGIIKAETLEDSRPGDVLKTHLGREIYVLKPGLSD 60 Query: 66 YLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGRVVSYE 125 YL+ M+R I+ PKD +I AY GI G +V+AG G+G + ++LAN+VG G V +YE Sbjct: 61 YLELMERRCSILLPKDIGMICAYTGIVEGSRVVDAGTGAGTVAMYLANLVGESGHVTTYE 120 Query: 126 IREDFAKLAWENIKWAGFDDRVTIKLKDIYEGIEEENVDHVILDLPQPERVVEHAAKALK 185 IREDFA++A +NI GF + V +K +DI EGIEEE++D V LDLP+P ++E +L Sbjct: 121 IREDFAEIAEKNIAAFGFKN-VEVKNRDIKEGIEEEDLDLVFLDLPRPWELMEDVHDSLL 179 Query: 186 PGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECMRPRTTALVHTG 245 GG+ V Y P QV +H K F RT + + EV+K+ RPRT + HTG Sbjct: 180 RGGWAVFYNPYIEQVKLIHR--IGLKVGFADIRTFETIEREIEVRKQGTRPRTRMVGHTG 237 Query: 246 YITFARRI 253 Y+TF R+I Sbjct: 238 YLTFMRKI 245 >sp|Q57598|Y134_METJA HYPOTHETICAL PROTEIN MJ0134 >gi|2127995|pir||F64316 hypothetical protein homolog MJ0134 - Methanococcus jannaschii >gi|1590899|gb|AAB98115.1| (U67470) L-isoaspartyl protein carboxyl methyltransferase isolog (pimT) [Methanococcus jannaschii] Length = 282 Score = 186 bits (468), Expect = 2e-46 Identities = 112/269 (41%), Positives = 158/269 (58%), Gaps = 31/269 (11%) Query: 9 VLVDPRGKRYLITVSKRDFHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILRPRIVDYLD 68 +LVD RGKRYL+ + F TDLGI+ +++I G +KSHKGH F ++ P + D L Sbjct: 6 LLVDERGKRYLLKKNVEKFGTDLGIVDMKDI---EEGVELKSHKGHTFYLVEPTMFDILK 62 Query: 69 KMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGRVVSYEIRE 128 +MKR + PKD I+A AGI G+ +VEAG GSGALT++L+N VG G+V++Y+IR Sbjct: 63 RMKRTVTTLLPKDIGFIIARAGIREGETVVEAGTGSGALTMYLSNAVGKTGKVITYDIRP 122 Query: 129 DFAKLAWENIKWAG--------------FDD-----------RVTIKLKDIYEGIEEENV 163 +FAK+A +N+ G FDD V K+ D+ E I+E++V Sbjct: 123 EFAKVARKNLLRVGAIKKGQKIIGLDEEFDDEDEIEIEDGLFNVIQKIGDVREKIDEKDV 182 Query: 164 DHVILDLPQPERVVEHAAKAL-KPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINV 222 D ++LDLP P VVE+A KAL K G V Y P QV + EKL+E + F T + Sbjct: 183 DVIVLDLPDPWNVVENAKKALNKKRGRIVTYLPYIEQVKKTVEKLKE--EGFWDIHTYEI 240 Query: 223 LVFDQEVKKECMRPRTTALVHTGYITFAR 251 + + E+ ++ +RP T + HTGYIT AR Sbjct: 241 IEREIEISEKGVRPSTRMIGHTGYITVAR 269 >pir||G72340 conserved hypothetical protein - Thermotoga maritima (strain MSB8) >gi|4981273|gb|AAD35829.1|AE001744_19 (AE001744) conserved hypothetical protein [Thermotoga maritima] Length = 265 Score = 172 bits (431), Expect = 4e-42 Identities = 96/253 (37%), Positives = 146/253 (56%), Gaps = 3/253 (1%) Query: 2 IREGDKVVLVDPRGKRYLITVSK-RDFHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILR 60 ++ GD+V+L +L+ + K + HT LGI+ L E+ + GE I++ G + IL Sbjct: 8 LKPGDRVLLSFEDESEFLVDLEKDKKLHTHLGIIDLNEVFEKGPGEIIRTSAGKKGYILI 67 Query: 61 PRIVDYLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGR 120 P ++D + MKR QIV+PKD++ I + GD I++ GVGSGA+ LA VG G+ Sbjct: 68 PSLIDEIMNMKRRTQIVYPKDSSFIAMMLDVKEGDRIIDTGVGSGAMCAVLARAVGSSGK 127 Query: 121 VVSYEIREDFAKLAWENIKWAGFDDRVTIKLKDIYEGIEEENVDHVILDLPQPERVVEHA 180 V +YE RE+FAKLA N+ G +RVTIK++DI EG +E++VD + LD+P P ++ Sbjct: 128 VFAYEKREEFAKLAESNLTKWGLIERVTIKVRDISEGFDEKDVDALFLDVPDPWNYIDKC 187 Query: 181 AKALKPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECMRPRTTA 240 +ALK GG F P +NQV +KL+E F++ L + E +RP Sbjct: 188 WEALKGGGRFATVCPTTNQVQETLKKLQELP--FIRIEVWESLFRPYKPVPERLRPVDRM 245 Query: 241 LVHTGYITFARRI 253 + HT Y+ FA ++ Sbjct: 246 VAHTAYMIFATKV 258 >emb|CAB59504.1| (AL132648) hypothetical protein SCI41.34c [Streptomyces coelicolor A3(2)] Length = 300 Score = 164 bits (411), Expect = 1e-39 Identities = 91/252 (36%), Positives = 145/252 (57%), Gaps = 4/252 (1%) Query: 5 GDKVVLVDPRGKRYLITVSK-RDFHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILRPRI 63 GD+V L DP+G+ Y T+ ++FHT G +E+IG G +++ + LRP + Sbjct: 17 GDQVQLTDPKGRHYTFTLEAGKNFHTHKGSFPHDELIGAPEGSVVRTTGNVAYLALRPLL 76 Query: 64 VDYLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGRVVS 123 DY+ M RG +V+PKDA I+A+A I PG +VEAGVGSG+L+ FL +G +G + S Sbjct: 77 PDYVLSMPRGAAVVYPKDAGQILAFADIFPGARVVEAGVGSGSLSSFLLRAIGDQGMLHS 136 Query: 124 YEIREDFAKLAWENIK--WAGFDDRVTIKLKDIYEGIEEENVDHVILDLPQPERVVEHAA 181 YE REDFA++A +N++ + G + + D+ + + + +VD VILD+ P ++ Sbjct: 137 YERREDFAEIAQQNVQRYFGGPHPAWQLTVGDLQDNLSDTDVDRVILDMLAPWECLDVVK 196 Query: 182 KALKPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECMRPRTTAL 241 KAL PGG Y + Q+ R E +RE F +P + ++ + V+ +RP + Sbjct: 197 KALVPGGILCCYVATTTQLARTVESIREI-GCFNEPTSWETMIRNWHVEGLAVRPDHRMI 255 Query: 242 VHTGYITFARRI 253 HTG++ ARR+ Sbjct: 256 GHTGFLLTARRL 267 >gb|AAC68688.1| (AF088800) unknown [Rhodococcus erythropolis] Length = 324 Score = 160 bits (400), Expect = 2e-38 Identities = 96/258 (37%), Positives = 148/258 (57%), Gaps = 9/258 (3%) Query: 3 REGDKVVLVDPRGKRYLITV-SKRDFHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILRP 61 R GD+V L D +G+ Y I + + R++HT G + ++IG + G +KS G + LRP Sbjct: 47 RVGDRVQLTDGKGRHYTIVLEANREYHTHKGGIVHNDLIGADEGSVVKSVNGTPYLALRP 106 Query: 62 RIVDYLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGRV 121 +VDY+ M RG Q+++PKDAA IV + PG ++EAG GSGALT L VGPEG+V Sbjct: 107 LLVDYVLSMPRGAQVIYPKDAAQIVHEGDVFPGARVLEAGAGSGALTCSLLRAVGPEGKV 166 Query: 122 VSYEIREDFAKLAWENIKWAGFDDRV---TIKLKDIYEGIEEEN---VDHVILDLPQPER 175 +SYE+R+D A+ A N++ F +R + + D+ E E + VD V+LD+ P Sbjct: 167 ISYEVRDDHAEHAVRNVE-TFFGERPPNWELTIADLAEFDPEAHGGKVDRVVLDMLAPWD 225 Query: 176 VVEHAAKALKPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECMR 235 V+ +KAL PGG + Y Q+ ++ E +RE ++ + +PR+ +V V +R Sbjct: 226 VLPAVSKALVPGGVLIVYVATVTQLSKVVEAMRE-QECWTEPRSWESIVRGWHVVGLAVR 284 Query: 236 PRTTALVHTGYITFARRI 253 P HT ++ ARR+ Sbjct: 285 PEHRMQGHTAFLVSARRL 302 >pir||A70513 hypothetical protein Rv2118c - Mycobacterium tuberculosis (strain H37RV) >gi|2253058|emb|CAB10715.1| (Z97559) hypothetical protein Rv2118c [Mycobacterium tuberculosis] Length = 280 Score = 154 bits (386), Expect = 8e-37 Identities = 91/253 (35%), Positives = 141/253 (54%), Gaps = 5/253 (1%) Query: 5 GDKVVLVDPRGKRYLITVSK-RDFHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILRPRI 63 G++V L D +G+RY ++++ +FHT G + + +IG G +KS G F +LRP + Sbjct: 10 GERVQLTDAKGRRYTMSLTPGAEFHTHRGSIAHDAVIGLEQGSVVKSSNGALFLVLRPLL 69 Query: 64 VDYLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGRVVS 123 VDY+ M RGPQ+++PKDAA IV I PG ++EAG GSGALTL L VGP G+V+S Sbjct: 70 VDYVMSMPRGPQVIYPKDAAQIVHEGDIFPGARVLEAGAGSGALTLSLLRAVGPAGQVIS 129 Query: 124 YEIREDFAKLAWENIK--WAGFDDRVTIKLKDIYEG-IEEENVDHVILDLPQPERVVEHA 180 YE R D A+ A N+ + D + + D+ + + + +VD +LD+ P V++ Sbjct: 130 YEQRADHAEHARRNVSGCYGQPPDNWRLVVSDLADSELPDGSVDRAVLDMLAPWEVLDAV 189 Query: 181 AKALKPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECMRPRTTA 240 ++ L GG + Y Q+ R+ E LR K + +PR L V +RP+ + Sbjct: 190 SRLLVAGGVLMVYVATVTQLSRIVEALRA-KQCWTEPRAWETLQRGWNVVGLAVRPQHSM 248 Query: 241 LVHTGYITFARRI 253 HT ++ RR+ Sbjct: 249 RGHTAFLVATRRL 261 >emb|CAA22923.1| (AL035310) hypothetical protein MLCB2533.09 [Mycobacterium leprae] Length = 281 Score = 150 bits (374), Expect = 2e-35 Identities = 91/259 (35%), Positives = 144/259 (55%), Gaps = 11/259 (4%) Query: 5 GDKVVLVDPRGKRYLITVSKR-DFHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILRPRI 63 G++V L D +G+ Y ++++ +FHT G + + IIG G +KS G F +LRP + Sbjct: 10 GERVQLTDTKGRHYTMSLTPGGEFHTHRGSIAHDGIIGLEQGSVVKSSTGALFLVLRPLL 69 Query: 64 VDYLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGRVVS 123 +DY+ M RGPQ+++PKDAA IV I PG ++EAG GSGALT L VGP+G+V+S Sbjct: 70 IDYVMSMPRGPQVIYPKDAAQIVHEGDIFPGARVLEAGAGSGALTCSLLRAVGPQGQVIS 129 Query: 124 YEIREDFAKLAWENIK----WAGFDDRVT----IKLKDIYEG-IEEENVDHVILDLPQPE 174 YE+R D A+ A N+ +G + R++ + + D+ + + +VD V+LD+ P Sbjct: 130 YELRTDHAEHARRNVSNFFCVSGAEGRLSDNWQLIVSDVANSELPDGSVDRVVLDMLAPW 189 Query: 175 RVVEHAAKALKPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECM 234 V++ ++ + GG V Y Q+ R E LR + +++PR L V + Sbjct: 190 EVLDTVSRLVIAGGVLVIYVATVTQLSRAVEALR-VQQCWVEPRAWETLQRGWNVVGLAV 248 Query: 235 RPRTTALVHTGYITFARRI 253 RP HT ++ ARR+ Sbjct: 249 RPEHLMRGHTAFLILARRL 267 >pir||S72844 beta-aspartate methyltransferase pimT - Mycobacterium leprae >gi|466999|gb|AAA17184.1| (U00017) pimT; B2126_C1_165 [Mycobacterium leprae] Length = 280 Score = 148 bits (371), Expect = 5e-35 Identities = 91/259 (35%), Positives = 143/259 (55%), Gaps = 11/259 (4%) Query: 5 GDKVVLVDPRGKRYLITVSKR-DFHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILRPRI 63 G++V L D +G+ Y ++++ +FHT G + + IIG G +KS G F +LRP + Sbjct: 10 GERVQLTDTKGRHYTMSLTPGGEFHTHRGSIAHDGIIGLEQGSVVKSSTGALFLVLRPLL 69 Query: 64 VDYLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGRVVS 123 DY+ M RGPQ+++PKDAA IV I PG ++EAG GSGALT L VGP+G+V+S Sbjct: 70 TDYVMSMPRGPQVIYPKDAAQIVHEGDIFPGARVLEAGAGSGALTCSLLRAVGPQGQVIS 129 Query: 124 YEIREDFAKLAWENIK----WAGFDDRVT----IKLKDIYEG-IEEENVDHVILDLPQPE 174 YE+R D A+ A N+ +G + R++ + + D+ + + +VD V+LD+ P Sbjct: 130 YELRTDHAEHARRNVSNFFCVSGAEGRLSDNWQLIVSDVANSELPDGSVDRVVLDMLAPW 189 Query: 175 RVVEHAAKALKPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECM 234 V++ ++ + GG V Y Q+ R E LR + +++PR L V + Sbjct: 190 EVLDTVSRLVIAGGVLVIYVATVTQLSRAVEALR-VQQCWVEPRAWETLQRGWNVVGLAV 248 Query: 235 RPRTTALVHTGYITFARRI 253 RP HT ++ ARR+ Sbjct: 249 RPEHLMRGHTAFLILARRL 267 >gb|AAK40752.1| Protein L-isoaspartate methyltransferase [Sulfolobus solfataricus] Length = 256 Score = 145 bits (361), Expect = 7e-34 Identities = 82/255 (32%), Positives = 140/255 (54%), Gaps = 8/255 (3%) Query: 2 IREGDKVVL-VDPRGKRYLITVSKRD--FHTDLGILKLEEIIGRNFGEAIKSHKGHEFKI 58 + EGD VV+ +DP KR I+ +R TD G++ + +IG +G ++ G + + Sbjct: 4 LNEGDPVVIWIDP--KRVYISKLERGKRLDTDKGVVFYDNLIGLEYGTSLTLKTGVKAYL 61 Query: 59 LRPRIVDYLDK-MKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGP 117 L+P I D K +KR Q+++PKD I+ ++ ++EAG GSG LT+ LA + Sbjct: 62 LKPTIQDLYSKGLKRPSQVLYPKDIGYILTTLSLNQVKTVIEAGTGSGFLTISLALSLSE 121 Query: 118 EGRVVSYEIREDFAKLAWENIKWAGFDDRVTIKLKDIYEGIEEENVDHVILDLPQPERVV 177 ++ +Y+IRED K+A N G DR+ KLKDI EGI+E+ VD + LD+P P + Sbjct: 122 NAKIYTYDIREDMQKVAKFNANVLGVQDRIVFKLKDIREGIDEKEVDAIFLDMPDPWNAI 181 Query: 178 EHAAKALKPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECMRPR 237 ++LKP + + P NQ+ + + ++ + +++ + +VK+ +RPR Sbjct: 182 PKLYESLKPSSTIIIFVPTVNQIEKTYFAMK--NSGIIDIHVEELILREYQVKENAIRPR 239 Query: 238 TTALVHTGYITFARR 252 ++HTG+I R+ Sbjct: 240 NIGIMHTGFIIRGRK 254 >gi|11497832 L-isoaspartyl protein carboxyl methyltransferase PimT, putative [Archaeoglobus fulgidus] >gi|7483853|pir||H69276 L-isoaspartyl-protein carboxyl methyltransferase PimT homolog - Archaeoglobus fulgidus >gi|2650427|gb|AAB91017.1| (AE001091) L-isoaspartyl protein carboxyl methyltransferase PimT, putative [Archaeoglobus fulgidus] Length = 247 Score = 122 bits (303), Expect = 4e-27 Identities = 79/248 (31%), Positives = 128/248 (50%), Gaps = 8/248 (3%) Query: 7 KVVLVDPRGK-RYLITVSKRDFHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILRPRIVD 65 K +V RGK +L+ + HT GI+ LEE+ G+++G+ +++H G +++I D Sbjct: 2 KPPVVLTRGKYSFLVDRFEGKLHTHHGIINLEELRGKDYGDEVRTHLGVKYRIAPFNPAD 61 Query: 66 YLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGRVVSYE 125 + KRG + PKD I+AY G+SP I +AG GSG L +LA G VV+ E Sbjct: 62 FFRHFKRGATPIMPKDIGAIIAYTGLSPDSLIFDAGTGSGILAAYLA-YFNKYGEVVTVE 120 Query: 126 IREDFAKLAWENIKWAGFDD--RVTIKLKDIYEGIEEENVDHVILDLPQPERVVEHAAKA 183 R DFAK+A EN + AG + ++ + + ++ E D + LD+ + A + Sbjct: 121 KRPDFAKIARENFRTAGLKNIHQIVGDALAVADSLKVE-FDLITLDMKDDVAFIRKAKEI 179 Query: 184 LKPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECMRPRTTALVH 243 LKPGG V Y P ++ ++ + F + +L D ++K+ R +T + H Sbjct: 180 LKPGGHIVVYNPYIEAAREVYLEME--RRGFKELEAFELLRVDLDIKRVGTR-TSTKVWH 236 Query: 244 TGYITFAR 251 TGY+ F R Sbjct: 237 TGYLVFGR 244 >pir||T48633 hypothetical protein T15N1.90 - Arabidopsis thaliana >gi|7573309|emb|CAB87627.1| (AL163792) putative protein [Arabidopsis thaliana] Length = 370 Score = 116 bits (287), Expect = 3e-25 Identities = 80/235 (34%), Positives = 119/235 (50%), Gaps = 8/235 (3%) Query: 2 IREGDKVVLVDPRGKRYLITVSKRD-FHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILR 60 I +GD V++ + + VSK G+ K + IG+ G + S+KG +L Sbjct: 16 IEDGDLVIVYERHDVMKPVKVSKDGVLQNRFGVYKHSDWIGKPLGTKVFSNKGKFVYLLA 75 Query: 61 PRIVDYLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGR 120 P + + QI++ D + +V Y + PG ++E+G GSG+L+ LA V P G Sbjct: 76 PTPELWTLVLSHRTQILYIADISFVVMYLEVVPGCVVLESGTGSGSLSTSLARAVAPTGH 135 Query: 121 VVSYEIREDFAKLAWENIKWAGFDDRVTIKLKDIY-EGIEEE---NVDHVILDLPQPERV 176 V S++ E A A E+ + G VT++++DI EG E+ D V LDLPQP Sbjct: 136 VYSFDFHEQRAVSAREDFEKTGISSLVTVEVRDIQGEGFPEKLSGLADSVFLDLPQPWLA 195 Query: 177 VEHAAKALKPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKK 231 V AAK LK G +++PC QV R E LR F++ RT VL+ EVK+ Sbjct: 196 VPSAAKMLKQDGVLCSFSPCIEQVQRTCEVLR---SDFIEIRTFEVLLRTYEVKE 247 >gi|8923586 hypothetical protein FLJ20628 [Homo sapiens] >gi|7020859|dbj|BAA91300.1| (AK000635) unnamed protein product [Homo sapiens] Length = 477 Score = 106 bits (263), Expect = 2e-22 Identities = 67/194 (34%), Positives = 104/194 (53%), Gaps = 13/194 (6%) Query: 28 HTDLGILKLEEIIGRNFGEAIKSHKGHEFKILRPRIVDYLDKMKRGPQIVHPKDAALIVA 87 +++ G + +I+G+ G+ ++S G ++ + RP + DY+ MKRG I PKD +I++ Sbjct: 181 NSNWGAVPFGKIVGKFPGQILRSSFGKQYMLRRPALEDYVVLMKRGTAITFPKDINMILS 240 Query: 88 YAGISPGDFIVEAGVGSGALTLFLANIVGPEGRVVSYEIREDFAKLAWENIK-WAG---- 142 I+PGD ++EAG GSG ++LFL+ VG +GRV+S+E+R+D LA +N K W Sbjct: 241 MMDINPGDTVLEAGSGSGGMSLFLSKAVGSQGRVISFEVRKDHHDLAKKNYKHWRDSWKL 300 Query: 143 -----FDDRVTIKLKDI---YEGIEEENVDHVILDLPQPERVVEHAAKALKPGGFFVAYT 194 + D V KDI E I+ D V LD+ P + LK GG Y Sbjct: 301 SHVEEWPDNVDFIHKDISGATEDIKSLTFDAVALDMLNPHVTLPVFYPHLKHGGVCAVYV 360 Query: 195 PCSNQVMRLHEKLR 208 QV+ L + +R Sbjct: 361 VNITQVIELLDGIR 374 >pir||F72480 probable beta-aspartate metyltransferase APE2486 - Aeropyrum pernix (strain K1) >gi|5106191|dbj|BAA81502.1| (AP000064) 261aa long hypothetical beta-aspartate metyltransferase [Aeropyrum pernix] Length = 261 Score = 104 bits (258), Expect = 8e-22 Identities = 72/259 (27%), Positives = 129/259 (49%), Gaps = 13/259 (5%) Query: 4 EGDKVVLV--DPRGKR--YLITVSKRD-FHTDLGILKLEEIIGRNFGEAIKSHKGHEFKI 58 +G VL+ +G+R Y + VS+ + T G++ I+G+ +G ++S G + Sbjct: 3 QGCPAVLIAESAKGERRSYYVRVSEGSAYSTIAGVVTGRSILGKPWGVILRSRLG-TIAV 61 Query: 59 LRPRIVDYLDKM-KRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGP 117 ++P + + ++ +R Q+++PKD+ +I G PG ++EAGVGSG +T LA + P Sbjct: 62 VKPSLAELMEGFYERRTQVIYPKDSGMIAVLVGARPGARLLEAGVGSGFMTTVLAMGLCP 121 Query: 118 EGRVVSYEIREDFAKLAWENIKWAGFDDRVTIKLKDIYEGIEEENV---DHVILDLPQPE 174 GR++ E+R + + A N++ AG D V ++L D+ E + D V+LD+P P Sbjct: 122 TGRLIGLEVRSENLETARRNLEAAGLLDCVDLRLGDVRNPASVEGLGPFDGVVLDMPDPW 181 Query: 175 RVVEHAAKALKPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECM 234 R +E + +KP V + P S Q+ +L + ++ + + + Sbjct: 182 RALESISGEVKPSAVAVVFVPTSTQLEKL---ITNMPGGWVLQGAFETMARTVLMSPGAV 238 Query: 235 RPRTTALVHTGYITFARRI 253 RP TGYI RR+ Sbjct: 239 RPGEWLAGFTGYIVVLRRV 257 >gb|AAF56565.1| (AE003754) CG14544 gene product [Drosophila melanogaster] Length = 317 Score = 99.8 bits (245), Expect = 3e-20 Identities = 76/264 (28%), Positives = 123/264 (45%), Gaps = 41/264 (15%) Query: 27 FHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILRPRIVDYLDKMKRGPQIVHPKDAALIV 86 F T+ G LK+E IIG +G ++ KG +L+P + + QI++ D ++I+ Sbjct: 46 FQTNYGSLKVENIIGVEYGSKVELSKGWAH-VLQPTPELWTQTLPHRTQIIYTPDISMII 104 Query: 87 AYAGISPGDFIVEAGVGSGALTLFLANIVGPEGRVVSYEIREDFAKLAWENIKWAGFDDR 146 + PG +VE+G GSG+L+ + + P G + +++ E A A + + G D Sbjct: 105 HQLEVRPGAVVVESGTGSGSLSHYFLRALKPTGHLHTFDFHEARADQARDEFRRHGIADF 164 Query: 147 VTIKLKDIYE---GIE-EENVDHVILDLPQPERVVEHAAKALK-PGGFFVAYTPCSNQVM 201 VT+ +D+ G E + D V LDLP P+ V HA KALK GG F +++PC Q Sbjct: 165 VTVYHRDVCNLGFGAELDGKADAVFLDLPAPDLAVPHAFKALKLSGGRFCSFSPCIEQSQ 224 Query: 202 RLHEKLREF------------KDYFMKPRTINVLVFD----------------------Q 227 R ++L + ++ +K RT+ V+ + + Sbjct: 225 RCIQELTKLGFNEIVSLEVLQQENVVKTRTLPVIDLEFLKLPKTDETTAATNALESKAPK 284 Query: 228 EVKKECMRPRTTALV-HTGYITFA 250 EVKK L HTG++TFA Sbjct: 285 EVKKYLTSSNPQTLPGHTGFLTFA 308 >gb|AAG18973.1| (AE004998) L-isoaspartyl protein carboxyl methyltransferase; PimT2 [Halobacterium sp. NRC-1] Length = 238 Score = 99.1 bits (243), Expect = 5e-20 Identities = 67/239 (28%), Positives = 109/239 (45%), Gaps = 8/239 (3%) Query: 14 RGKRYLITVSKRDFHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILRPRIVDYLDKMKRG 73 RG R + + HTDLG+++ + GE +++H G F + R D + +R Sbjct: 5 RGDREFLVAPGEELHTDLGVIEAPD--DPTPGETVETHLGEPFTVRELRGPDLFNHFERT 62 Query: 74 PQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGRVVSYEIREDFAKL 133 + PKD L++ + G+S D +++AG G+G L +L G + V +YE DFA + Sbjct: 63 GAPMMPKDIGLVMGHTGVSGADRVLDAGTGTGVLAAYLGR-AGAD--VTTYEQDADFAAV 119 Query: 134 AWENIKWAGFDDRVTIKLKDIYEGIEE-ENVDHVILDLPQPERVVEHAAKALKPGGFFVA 192 A EN+ AG D V+++ D+ ++ D + LD VV HA L GG Sbjct: 120 ARENMSLAGVSDAVSVRAGDVTADLDALGTFDVLTLDTQDAPDVVAHADDLLVSGGQVAV 179 Query: 193 YTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECMRPRTTALVHTGYITFAR 251 Y+P + R+ + + + + RP T + HTGY+ FAR Sbjct: 180 YSPFVENARATTQAARDAG--LVDIDAMETIQRRMDFDDRGSRPSTAGVGHTGYLLFAR 236 >pir||T24625 hypothetical protein W02A11.1 - Caenorhabditis elegans >gi|3879583|emb|CAB04711.1| (Z81587) cDNA EST yk56g3.3 comes from this gene~cDNA EST yk56g3.5 comes from this gene~cDNA EST yk213d3.3 comes from this gene~cDNA EST yk213d3.5 comes from this gene [Caenorhabditis elegans] >gi|3880444|emb|CAB04894.1| (Z82062) cDNA EST yk56g3.3 comes from this gene~cDNA EST yk56g3.5 comes from this gene~cDNA EST yk213d3.3 comes from this gene~cDNA EST yk213d3.5 comes from this gene [Caenorhabditis elegans] Length = 312 Score = 89.7 bits (219), Expect = 3e-17 Identities = 64/206 (31%), Positives = 103/206 (49%), Gaps = 6/206 (2%) Query: 2 IREGDKVVLVDPRGKRYLITVSK-RDFHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILR 60 I+EGD V++ G+ V + + G L+ E IIG+ +G+ + + G+ + ILR Sbjct: 15 IQEGDTVIVYVTYGQMVPTIVKRGQTLMMKYGALRHEFIIGKRWGQRLSATAGYVY-ILR 73 Query: 61 PRIVDYLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGR 120 P + + R QI++ D A I++ PG I E+G GSG+L+ +A V P G Sbjct: 74 PTSDLWTRALPRRTQILYTADCAQILSLLDAKPGSVICESGTGSGSLSHAIALTVAPTGH 133 Query: 121 VVSYEIREDFAKLAWENIKWAGFDDRVTIKLKDI-YEGIEEEN-VDHVILDLPQPERVVE 178 + +++I E E K G T ++++ G EN D V LD+P P + Sbjct: 134 LYTHDIDETRTHKVLEEFKVHGLGSVTTAVVRNVCTSGFHVENAADGVFLDVPAPWEAIP 193 Query: 179 HAAKAL--KPGGFFVAYTPCSNQVMR 202 AAK++ GG V+++PC QV R Sbjct: 194 FAAKSIFRARGGRLVSFSPCLEQVQR 219 >pir||A70328 hypothetical protein aq_311 - Aquifex aeolicus >gi|2983004|gb|AAC06616.1| (AE000683) putative protein [Aquifex aeolicus] Length = 248 Score = 88.5 bits (216), Expect = 7e-17 Identities = 67/253 (26%), Positives = 115/253 (44%), Gaps = 13/253 (5%) Query: 3 REGDKVVLVDPRGKRYLITVSKRDFHTDLGILKLEEIIGRNFGEAIKSHKGHEFKILRPR 62 +EG+ V++ K + K+ +LK +E+IG+ G I F++ RP Sbjct: 5 KEGEYVLIRFGEKKFLRKLLPKQSLSVKKSVLKFDEVIGKPEGVKING-----FEVYRPT 59 Query: 63 IVDY-LDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGRV 121 + + L +R QI++PKD+ I ++ ++E G GSGAL L+ + G V Sbjct: 60 LEEIILLGFERKTQIIYPKDSFYIALKLNLNKEKRVLEFGTGSGALLAVLSEVA---GEV 116 Query: 122 VSYEIREDFAKLAWENIKWAGFDDRVTIKLKDIYEGIEEENVDH-VILDLPQPERVVEHA 180 ++E E+F K A +N+K V D + E + H +D+ +P +E Sbjct: 117 WTFEAVEEFYKTAQKNLKKFNLGKNVKFFNVDFKDAEVPEGIFHAAFVDVREPWHYLEKV 176 Query: 181 AKALKPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECMRPRTTA 240 K+L G P +NQV++L L ++YF + +L + E RP Sbjct: 177 HKSLMEGAPVGFLLPTANQVIKL---LESIENYFGNLEVVEILHRHYKTISERFRPEDQM 233 Query: 241 LVHTGYITFARRI 253 + HT Y+ F R++ Sbjct: 234 VAHTAYLVFGRKL 246 >emb|CAC11981.1| (AL445065) conserved hypothetical protein [Thermoplasma acidophilum] Length = 255 Score = 78.8 bits (191), Expect = 6e-14 Identities = 53/181 (29%), Positives = 87/181 (47%), Gaps = 2/181 (1%) Query: 70 MKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEGRVVSYEIRED 129 ++R QI+ DA+ I+ G+ PG I+E GVGSG ++ ++ + +G + E ED Sbjct: 67 IRRNTQIISEIDASYIIMRCGLRPGMDILEVGVGSGNMSSYILYALNGKGTLTVVERDED 126 Query: 130 FAKLAWENIKWAGFDDRVTIKLKDIYEGIEEENVDHVILDLPQPERVVEHAAKALKPGGF 189 K A +N+ V DI + I ++ D VI D+P P V+ A +KPG Sbjct: 127 NLKKAMDNLSEFYDIGNVRTSRSDIADFISDQMYDAVIADIPDPWNHVQKIASMMKPGSV 186 Query: 190 FVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECMRPRTTALVHTGYITF 249 Y P +Q + L + ++ T+ ++ V++ RP + L HT +ITF Sbjct: 187 ATFYLPNFDQSEKTVLSLSASGMHHLE--TVELMKRRILVREGATRPASDDLTHTAFITF 244 Query: 250 A 250 A Sbjct: 245 A 245 >gb|AAC64273.1|AAC64273 (AF086832) unknown [Streptomyces coelicolor A3(2)] Length = 168 Score = 73.0 bits (176), Expect = 3e-12 Identities = 42/133 (31%), Positives = 72/133 (53%), Gaps = 3/133 (2%) Query: 123 SYEIREDFAKLAWENIK--WAGFDDRVTIKLKDIYEGIEEENVDHVILDLPQPERVVEHA 180 SYE REDFA++A +N++ + G + + D+ + + + +VD VILD+ P ++ Sbjct: 4 SYERREDFAEIAQQNVQRYFGGPHPAWQLTVGDLQDNLSDTDVDRVILDMLAPWECLDVV 63 Query: 181 AKALKPGGFFVAYTPCSNQVMRLHEKLREFKDYFMKPRTINVLVFDQEVKKECMRPRTTA 240 KAL PGG Y + Q+ R E +RE F +P + ++ + V+ +RP Sbjct: 64 KKALVPGGILCCYVATTTQLARTVESIREI-GCFNEPTSWETMIRNWHVEGLAVRPDHRM 122 Query: 241 LVHTGYITFARRI 253 + HTG++ ARR+ Sbjct: 123 IGHTGFLLTARRL 135 >pir||T39235 probable translational repressor - fission yeast (Schizosaccharomyces pombe) >gi|2370560|emb|CAB11496.1| (Z98763) putative translational repressor [Schizosaccharomyces pombe] Length = 364 Score = 68.7 bits (165), Expect = 7e-11 Identities = 59/220 (26%), Positives = 99/220 (44%), Gaps = 13/220 (5%) Query: 2 IREGDKVVLVDPRGKRYLITV-SKRDFHTDLGILKLEEIIGRNFGEAIKSHKGHEF-KIL 59 + GD V R K + + +++ FH G E+IG+ +GE I S F +L Sbjct: 10 VENGDLAVAWIGRNKLIPLHIEAEKTFHNQYGAFPHSEMIGKRYGEQIASTAKQGFIYLL 69 Query: 60 RPRIVDYLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEAGVGSGALTLFLANIVGPEG 119 +P + + QIV+ D ALI I+ G ++EAG GS +++ ++ VGP G Sbjct: 70 QPTPELWTLALPHRTQIVYTPDIALIHQKLRITYGTRVIEAGTGSASMSHAISRTVGPLG 129 Query: 120 RVVSYEIREDFAKLAWENIKW------AGFDDRVTIK--LKDIYEGIEEENVDHVILDLP 171 R+ ++E + A + + G + +T + KD + E + VD + LDLP Sbjct: 130 RLFTFEYHATRYQTALQEFREHEMLIDVGGNTHLTHRDVCKDGFLDTEVK-VDAIFLDLP 188 Query: 172 QPERVVEHAAKALK--PGGFFVAYTPCSNQVMRLHEKLRE 209 P + H + + ++PC Q+ E LRE Sbjct: 189 APWEAIPHLSNHVNHDKSTRICCFSPCIEQIQHSAEALRE 228 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.323 0.143 0.423 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 99182567 Number of Sequences: 2977 Number of extensions: 4291608 Number of successful extensions: 10953 Number of sequences better than 1.0e-10: 22 Number of HSP's better than 0.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 10895 Number of HSP's gapped (non-prelim): 22 length of query: 253 length of database: 189,106,746 effective HSP length: 52 effective length of query: 201 effective length of database: 157,985,422 effective search space: 31755069822 effective search space used: 31755069822 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.9 bits) S2: 164 (68.3 bits)