BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0287 (leuC-2) DE:3-isopropylmalate dehydratase, large
subunit (leuC-2)
(380 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||H75159 3-isopropylmalate dehydratase, large chain (leuc-2) ... 755 0.0
pir||A71181 probable isomerase - Pyrococcus horikoshii >gi|32581... 618 e-176
pir||C72394 hypothetical protein TM0291 - Thermotoga maritima (s... 302 6e-81
gi|11499781 3-isopropylmalate dehydratase, large subunit (leuC) ... 301 1e-80
pir||B75045 probable 3-isopropylmalate dehydratase (EC 4.2.1.33)... 295 5e-79
dbj|BAA74762.1| (AB017109) HacA [Thermus thermophilus] 294 1e-78
gi|11499545 aconitase (acn) [Archaeoglobus fulgidus] >gi|3122334... 294 1e-78
sp|O27668|LE22_METTH PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE LARG... 286 4e-76
gb|AAK42608.1| 3-isopropylmalate dehydratase, large subunit (iso... 283 3e-75
sp|O27439|LE21_METTH PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE LARG... 282 4e-75
pir||H72362 3-isopropylmalate dehydratase, large subunit - Therm... 282 6e-75
pir||D75373 3-isopropylmalate dehydratase, large subunit - Deino... 275 6e-73
sp|Q58409|LE21_METJA PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE LARG... 270 2e-71
sp|O67078|LEU2_AQUAE 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 268 6e-71
sp|P81291|LE22_METJA PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE LARG... 253 3e-66
pir||T51171 homoaconitate hydratase (EC 4.2.1.36) [validated] - ... 238 1e-61
pir||H75354 3-isopropylmalate dehydratase, large subunit - Deino... 231 1e-59
pir||F70453 aconitase - Aquifex aeolicus >gi|2984078|gb|AAC07617... 213 3e-54
gb|AAG20620.1| (AE005132) aconitase; Can [Halobacterium sp. NRC-1] 195 6e-49
pir||T06300 hypothetical protein T9E8.170 - Arabidopsis thaliana... 189 5e-47
pir||T38665 probable homoaconitate hydratase (EC 4.2.1.36) - fis... 174 2e-42
sp|P80858|LEU2_BACSU 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 164 2e-39
gi|6320440 homoaconitase; Lys4p [Saccharomyces cerevisiae] >gi|1... 158 9e-38
sp|Q92412|LYS4_EMENI HOMOACONITASE PRECURSOR (HOMOACONITATE HYDR... 157 1e-37
dbj|BAB06775.1| (AP001517) 3-isopropylmalate dehydratase large s... 157 3e-37
sp|O53237|LEU2_MYCTU 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 150 2e-35
sp|O33123|LEU2_MYCLE 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 145 6e-34
pir||A81270 3-isopropylmalate dehydratase (EC 4.2.1.33) large ch... 142 6e-33
sp|Q44427|LEU2_ACTTI 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 141 2e-32
pir||T29083 3-isopropylmalate dehydratase (EC 4.2.1.33) large ch... 141 2e-32
gi|10954460 3-isopropylmalate isomerase subunit [Buchnera aphidi... 140 2e-32
gi|10954452 isopropylmalate isomerase subunit [Buchnera aphidico... 140 2e-32
dbj|BAA37138.1| (AB017135) isopropylmalate isomerase large subun... 139 4e-32
sp|Q02142|LEU2_LACLA 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 136 3e-31
gi|10957109 3-isopropylmalate dehydratase [Buchnera sp. APS] >gi... 136 4e-31
sp|P54384|LEU2_SYNY3 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 135 9e-31
sp|P56934|LEU2_BUCAI 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 134 1e-30
emb|CAC14578.1| (AJ296268) 2-isopropylmalate isomerase large sub... 133 3e-30
sp|P48573|LEU2_BUCRP 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 132 8e-30
pir||G81128 3-isopropylmalate dehydratase, large chain NMB1036 [... 131 1e-29
pir||A81836 probable 3-isopropylmalate dehydratase (EC 4.2.1.33)... 131 2e-29
pir||D36889 3-isopropylmalate dehydratase (EC 4.2.1.33) chain le... 131 2e-29
gb|AAG31400.1| (AF197455) isopropylmaltate dehydratase subunit [... 129 7e-29
gb|AAG31391.1| (AF197452) isopropylmaltate dehydratase subunit [... 128 9e-29
gb|AAG31397.1| (AF197454) isopropylmaltate dehydratase subunit [... 128 1e-28
pir||H82070 3-isopropylmalate dehydratase, large chain VC2492 [s... 128 1e-28
sp|P49601|LEU2_USTMA 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMAL... 127 2e-28
sp|P55811|LEU2_RHINI 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMAL... 127 2e-28
sp|P44968|LEU2_HAEIN 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 126 5e-28
gb|AAG31382.1| (AF197449) isopropylmaltate dehydratase subunit [... 125 6e-28
sp|O14289|LEU2_SCHPO 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMAL... 125 8e-28
pir||S40586 3-isopropylmalate dehydratase (EC 4.2.1.33) alpha ch... 125 8e-28
sp|P30127|LEU2_ECOLI 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 125 8e-28
pir||H64728 3-isopropylmalate dehydratase (EC 4.2.1.33) alpha ch... 124 1e-27
gb|AAG31388.1| (AF197451) isopropylmaltate dehydratase subunit [... 123 2e-27
emb|CAB83173.1| (AL162692) aconitate hydratase [Schizosaccharomy... 123 3e-27
gb|AAG31406.1| (AF197457) isopropylmaltate dehydratase subunit [... 123 3e-27
pir||S26864 3-isopropylmalate dehydratase (EC 4.2.1.33) - Phycom... 123 4e-27
sp|P18250|LEU2_PHYBL 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMAL... 123 4e-27
sp|P17279|LEU2_RHIRA 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMAL... 123 4e-27
gb|AAG31379.1| (AF197448) isopropylmaltate dehydratase subunit [... 122 5e-27
sp|Q00464|LEU2_CANMA 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMAL... 120 2e-26
sp|P55251|LEU2_RHIPU 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMAL... 119 5e-26
pir||G83255 3-isopropylmalate dehydratase large subunit PA3121 [... 119 5e-26
gi|6321429 isopropylmalate isomerase; Leu1p [Saccharomyces cerev... 117 2e-25
gi|6323335 Aconitase, mitochondrial; Aco1p [Saccharomyces cerevi... 116 4e-25
gb|AAA34389.1| (M33131) aconitase (ACO1) (EC 4.2.1.3) [Saccharom... 116 4e-25
emb|CAB99455.1| (AJ278693) 3-isopropylmalate dehydratase [Yarrow... 116 4e-25
pir||H82564 3-isopropylmalate dehydratase large subunit XF2375 [... 115 7e-25
emb|CAC18623.1| (AL451109) related to aconitate hydratase precur... 115 9e-25
emb|CAA76360.1| (Y16747) aconitate hydratase [Piromyces sp. E2] 115 9e-25
sp|P34455|ACON_CAEEL PROBABLE ACONITATE HYDRATASE, MITOCHONDRIAL... 114 2e-24
sp|P20004|ACON_BOVIN ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSO... 114 2e-24
sp|P15717|LEU2_SALTY 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 114 2e-24
gb|AAB19612.1| (S57886) isopropylmalate isomerase {EC 4.2.1.33} ... 114 2e-24
pdb|5ACN| Aconitase (E.C.4.2.1.3) (Inactive (3fe-4s) Cluster F... 112 8e-24
pdb|1C96|A Chain A, S642a:citrate Complex Of Aconitase 112 8e-24
pdb|1C97|A Chain A, S642a:isocitrate Complex Of Aconitase >gi|67... 112 8e-24
pdb|1B0J|A Chain A, Crystal Structure Of Aconitase With Isocitrate 112 8e-24
pdb|1B0M|A Chain A, Aconitase R644q:fluorocitrate Complex 112 8e-24
sp|P16276|ACON_PIG ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSOR ... 112 8e-24
pdb|8ACN| Aconitase (E.C.4.2.1.3) Complex With Nitroisocitrate... 111 1e-23
pdb|1NIS| Aconitase (E.C.4.2.1.3) Complex With Nitrocitrate (M... 111 1e-23
pdb|1AMJ| Aconitase (E.C.4.2.1.3) Complexed With Sulfate And H... 111 1e-23
pir||S57528 aconitate hydratase (EC 4.2.1.3) precursor, mitochon... 111 1e-23
gb|AAB38416.1| (U80040) mitochondrial aconitase [Homo sapiens] 110 3e-23
gi|4501867 aconitase 2; Aconitase, mitochondrial [Homo sapiens] ... 110 3e-23
emb|CAB46022.1| (AL023553) dJ347H13.1 (aconitase 2, mitochondria... 110 3e-23
gb|AAD19351.2| (AF093096) aconitase [Homo sapiens] 109 7e-23
emb|CAC11018.1| (AJ243266) mitochondrial aconitase [Rattus norve... 109 7e-23
gb|AAF54588.1| (AE003690) CG4706 gene product [Drosophila melano... 108 9e-23
sp|P49609|ACON_GRAVE ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSO... 108 9e-23
gb|AAC61778.1| (AF093142) aconitase [Aspergillus terreus] 107 2e-22
gb|AAF01428.1|AF186471_1 (AF186471) aconitase [Xenopus laevis] 107 3e-22
sp|O13966|ACON_SCHPO ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSO... 106 3e-22
emb|CAB72317.1| (AJ245736) aconitase [Daphnia pulex] 104 2e-21
pir||S10171 3-isopropylmalate dehydratase (EC 4.2.1.33) chain le... 102 5e-21
emb|CAB93521.1| (AJ252018) mitochondrial aconitase [Drosophila m... 99 6e-20
gb|AAF53973.1| (AE003669) Acon gene product [alt 1] [Drosophila ... 99 1e-19
emb|CAA89720.1| (Z49701) unknown [Saccharomyces cerevisiae] 98 2e-19
pir||S45196 3-isopropylmalate dehydrogenase homolog - Escherichi... 98 2e-19
sp|P36683|ACO2_ECOLI ACONITATE HYDRATASE 2 (CITRATE HYDRO-LYASE ... 98 2e-19
gb|AAC35557.1| (AF086790) aconitase precursor [Homo sapiens] 96 5e-19
pir||C82303 aconitate hydratase 2 VC0604 [imported] - Vibrio cho... 94 2e-18
gb|AAK41354.1| Aconitate hydratase [Sulfolobus solfataricus] 94 2e-18
sp|Q44023|LEU2_ALCEU 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 89 6e-17
gb|AAD32176.1|AF125563_1 (AF125563) putative aconitate hydratase... 89 8e-17
pir||G81068 aconitate hydratase 2 NMB1572 [imported] - Neisseria... 88 1e-16
pir||A81801 aconitate hydratase (EC 4.2.1.3) NMA1761 [imported] ... 88 1e-16
sp|P74582|ACO2_SYNY3 ACONITATE HYDRATASE 2 (CITRATE HYDRO-LYASE ... 86 6e-16
emb|CAA72703.1| (Y11966) 3-isopropylmalate dehydratase subunit [... 83 6e-15
pir||G83422 aconitate hydratase 2 PA1787 [imported] - Pseudomona... 81 2e-14
emb|CAA88513.1| (Z48612) unknown [Saccharomyces cerevisiae] 78 2e-13
pir||A82825 aconitate hydratase 2 XF0292 [imported] - Xylella fa... 77 3e-13
pir||C81356 aconitate hydratase (EC 4.2.1.3) Cj0835c [imported] ... 72 9e-12
>pir||H75159 3-isopropylmalate dehydratase, large chain (leuc-2) PAB0287 -
Pyrococcus abyssi (strain Orsay)
>gi|5457869|emb|CAB49359.1| (AJ248284) 3-isopropylmalate
dehydratase, large subunit (leuC-2) [Pyrococcus abyssi]
Length = 380
Score = 755 bits (1929), Expect = 0.0
Identities = 380/380 (100%), Positives = 380/380 (100%)
Query: 1 MTLVGKLLNAKPGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRTRAYIFFDHVYPAPT 60
MTLVGKLLNAKPGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRTRAYIFFDHVYPAPT
Sbjct: 1 MTLVGKLLNAKPGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRTRAYIFFDHVYPAPT 60
Query: 61 VKVANLHREIREFAKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVF 120
VKVANLHREIREFAKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVF
Sbjct: 61 VKVANLHREIREFAKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVF 120
Query: 121 AVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNA 180
AVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNA
Sbjct: 121 AVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNA 180
Query: 181 IEFFNVPFSFDERLTLTNFSVEANAKTAIIGEEYEGGGYSDEITIELDSLPPMVAKPFSP 240
IEFFNVPFSFDERLTLTNFSVEANAKTAIIGEEYEGGGYSDEITIELDSLPPMVAKPFSP
Sbjct: 181 IEFFNVPFSFDERLTLTNFSVEANAKTAIIGEEYEGGGYSDEITIELDSLPPMVAKPFSP 240
Query: 241 ANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQVNVRTLVAPASINVYRRMI 300
ANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQVNVRTLVAPASINVYRRMI
Sbjct: 241 ANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQVNVRTLVAPASINVYRRMI 300
Query: 301 EEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLAS 360
EEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLAS
Sbjct: 301 EEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLAS 360
Query: 361 PVTAAVSALYGEITNPEGEL 380
PVTAAVSALYGEITNPEGEL
Sbjct: 361 PVTAAVSALYGEITNPEGEL 380
>pir||A71181 probable isomerase - Pyrococcus horikoshii
>gi|3258157|dbj|BAA30840.1| (AP000007) 380aa long
hypothetical isomerase [Pyrococcus horikoshii]
Length = 380
Score = 618 bits (1576), Expect = e-176
Identities = 298/380 (78%), Positives = 339/380 (88%)
Query: 1 MTLVGKLLNAKPGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRTRAYIFFDHVYPAPT 60
MTLV ++L+AK GE VIR VDLVYAHDGTMPLIIEAFN++FN V+ RAY+FFDHVYPAPT
Sbjct: 1 MTLVEEILDAKAGEVVIRNVDLVYAHDGTMPLIIEAFNKVFNSVKARAYVFFDHVYPAPT 60
Query: 61 VKVANLHREIREFAKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVF 120
VKVANL +EIREFA+ HGIPVVEGKGISHQLVVE+GLAE+++I+VGGDSHTPTLGALGVF
Sbjct: 61 VKVANLQKEIREFARRHGIPVVEGKGISHQLVVEMGLAEKSRIVVGGDSHTPTLGALGVF 120
Query: 121 AVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNA 180
AVGMGATDVA+ LGLGK WL+VPES+AV+ +G P MA+DVMI LITSL++ +M+Y A
Sbjct: 121 AVGMGATDVAVVLGLGKTWLRVPESVAVIFEGKPSRTTMAADVMIRLITSLRNYDMNYKA 180
Query: 181 IEFFNVPFSFDERLTLTNFSVEANAKTAIIGEEYEGGGYSDEITIELDSLPPMVAKPFSP 240
+EFFNVPF+ DERLTLTNFSVEANAKTA+IGEEY+G Y EI+ EL SL PMVAKPFSP
Sbjct: 181 LEFFNVPFTADERLTLTNFSVEANAKTALIGEEYDGRNYIKEISFELSSLGPMVAKPFSP 240
Query: 241 ANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQVNVRTLVAPASINVYRRMI 300
ANGV VE VEGTKIDQVFIGSCTNGRFE IKRAAEIL GE+V VRT++ PAS+NVY+RM+
Sbjct: 241 ANGVEVEKVEGTKIDQVFIGSCTNGRFEHIKRAAEILKGEEVAVRTIIGPASVNVYKRMV 300
Query: 301 EEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLAS 360
EG+V IL+DAG ILPPGCGPCLGRHMGV GD EV+LSTTNRNFRGRMGSP AEIYLAS
Sbjct: 301 REGIVDILLDAGATILPPGCGPCLGRHMGVLGDKEVVLSTTNRNFRGRMGSPTAEIYLAS 360
Query: 361 PVTAAVSALYGEITNPEGEL 380
P+TAAVSALYGEIT+P GE+
Sbjct: 361 PLTAAVSALYGEITSPGGEV 380
>pir||C72394 hypothetical protein TM0291 - Thermotoga maritima (strain MSB8)
>gi|4980790|gb|AAD35379.1|AE001711_2 (AE001711)
3-isopropylmalate dehydratase, large subunit, putative
[Thermotoga maritima]
Length = 418
Score = 302 bits (764), Expect = 6e-81
Identities = 166/399 (41%), Positives = 243/399 (60%), Gaps = 32/399 (8%)
Query: 9 NAKPGEAVIRKVDLVYAHDGTMPLIIEAFNRI-FNEVRT-RAYIFFDHVYPAPTVKVANL 66
+ K GE V+ +VD+ A DGT PL+I F + F EV+ +A++F DH P+P +++N
Sbjct: 17 DVKAGEIVLARVDIAMAQDGTGPLMINEFRELGFKEVKVPKAFLFIDHASPSPRKELSNS 76
Query: 67 HREIREFAKIHGIPVVE-GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMG 125
+ +REF K G+ V + G GISHQ++ E + ++ G DSHT T G LG F GMG
Sbjct: 77 QKMMREFGKEMGVKVFDAGDGISHQILAEK-YVKPGDLVAGADSHTCTAGGLGAFGTGMG 135
Query: 126 ATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFN 185
+TDVAI GLG+ W KVPE+I V+++G ++ + A D+++ + L +Y A+EF
Sbjct: 136 STDVAIIFGLGQNWFKVPETIKVVVNGKLQDGVYAKDIILEIARILGSDGATYKALEFHG 195
Query: 186 V---PFSFDERLTLTNFSVEANAKTAII-------------GEEYE--------GGGYSD 221
+ ++RLT++N +VE AK ++ G E + Y
Sbjct: 196 SCIENMNVEDRLTISNMAVEVGAKAGLMPSDEKTREFLKKMGREEDFRELKADPDAVYET 255
Query: 222 EITIELDSLPPMVAKPFSPANGVSVEDVEGTKI--DQVFIGSCTNGRFEQIKRAAEILD- 278
EI I+ +L P+V+ P N V +VE KI DQVFIG+CTNGR + ++ A +IL+
Sbjct: 256 EIEIDATTLEPLVSLPHYVDNVRKVSEVEKEKIKIDQVFIGTCTNGRLQDLEIALKILEK 315
Query: 279 -GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVI 337
G+ +VR +V PAS VY +E+G+++ ++ G ++PPGCGPC+G HMGV GD E +
Sbjct: 316 HGKHPDVRLIVGPASRKVYMDALEKGIIKKFVELGAAVIPPGCGPCVGIHMGVLGDGERV 375
Query: 338 LSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNP 376
LST NRNF+GRMG+P AEIYLASP TAA +A+ G IT+P
Sbjct: 376 LSTQNRNFKGRMGNPNAEIYLASPATAAATAVTGYITDP 414
>gi|11499781 3-isopropylmalate dehydratase, large subunit (leuC) [Archaeoglobus
fulgidus] >gi|3122333|sp|O28084|LE22_ARCFU PUTATIVE
3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7450353|pir||G69524 3-isopropylmalate dehydratase,
large subunit (leuC) homolog - Archaeoglobus fulgidus
>gi|2648330|gb|AAB89057.1| (AE000954) 3-isopropylmalate
dehydratase, large subunit (leuC) [Archaeoglobus
fulgidus]
Length = 416
Score = 301 bits (762), Expect = 1e-80
Identities = 171/410 (41%), Positives = 243/410 (58%), Gaps = 36/410 (8%)
Query: 2 TLVGKLLNAKP------GEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRT--RAYIFFD 53
T+ K+L+ K G+ V+ ++D + DGT PL I + EVR R + F D
Sbjct: 4 TIAEKILSEKSKSDAYAGDIVVAEIDQIALQDGTAPLAIRQLMELGTEVRAADRTHFFVD 63
Query: 54 HVYPAPTVKVANLHREIREFAKIHGIPV-VEGKGISHQLVVELGLAEEAKIIVGGDSHTP 112
H P+P +++N + I EFAK G G+GI HQ++VE + + VG DSHT
Sbjct: 64 HAAPSPRRELSNDQKFIYEFAKKVGADFNPPGEGIIHQIMVER-YVKPGDLAVGADSHTC 122
Query: 113 TLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLK 172
T G +G F+ GMG+TDVA+A+ LGK W +VPES V LDGS + + A DV++ LI L
Sbjct: 123 TYGGIGAFSTGMGSTDVAVAIALGKNWFRVPESFRVQLDGSLPKGVFAKDVILKLIGDLG 182
Query: 173 DREMSYNAIEFFNV---PFSFDERLTLTNFSVEANAKTAI-------------IGEEY-- 214
+Y A+EF + +ERLT+ N +VE AK I +G E
Sbjct: 183 VDGATYKALEFHGECAENMTVEERLTIANMAVECGAKAGIFESDENTRKFLAELGREGDF 242
Query: 215 ------EGGGYSDEITIELDSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFE 268
E Y EI +++ SL P+V+KP + N + +VEGT+++QV+IG+CTNGR
Sbjct: 243 REVKADEDAEYEKEIYMDVSSLVPVVSKPHNVDNVAEISEVEGTEVNQVYIGTCTNGRLS 302
Query: 269 QIKRAAEILDGEQVN--VRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGR 326
++ AA IL G +V VR +V PAS VY + +++GL+R+ ++AG ++L PGCGPC+G
Sbjct: 303 DLEVAARILKGRKVKEGVRLIVVPASRRVYLQALDKGLIRVFVEAGGMVLNPGCGPCVGI 362
Query: 327 HMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNP 376
H G+ D EV +ST NRNF+GRMG+P AEI+LASP TAA SA+ G I +P
Sbjct: 363 HQGILADGEVCISTQNRNFKGRMGNPNAEIFLASPATAAASAVKGYIADP 412
>pir||B75045 probable 3-isopropylmalate dehydratase (EC 4.2.1.33) large chain
PAB0891 - Pyrococcus abyssi (strain Orsay)
>gi|5458768|emb|CAB50255.1| (AJ248287) PUTATIVE
3-ISOPROPYLMALATE DEHYDRATASE LARGE GE SUBUNIT (EC
4.2.1.33) (ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM
ISOMERASE) (IPMI). [Pyrococcus abyssi]
Length = 423
Score = 295 bits (748), Expect = 5e-79
Identities = 176/419 (42%), Positives = 241/419 (57%), Gaps = 44/419 (10%)
Query: 1 MTLVGKLL-------NAKPGEAVIRKVDLVYAHDGTMPLIIEAFN-----RIFNEVRTRA 48
MT+ K+L KPGE V+ K+D V+ +D TMPL I+ F R+F+ R R
Sbjct: 3 MTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFD--RERI 60
Query: 49 YIFFDHVYPAPTVKVANLHREIREFAKIHGIP-VVEGK--GISHQLVVELGLAEEAKIIV 105
I DH P +K A + REFAK GI EG G+ H L+ ELGL +I+
Sbjct: 61 AIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLII 120
Query: 106 GGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMI 165
G DSHT T GALG FA G+G+TD+A+A+ G+ W +VPE++ + +G + Y+ D+++
Sbjct: 121 GADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLIL 180
Query: 166 HLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNFSVEANAKTAIIGEEYEGGGY--- 219
H I + Y +EF S ++R+T++N ++EA AKT II + + Y
Sbjct: 181 HTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKE 240
Query: 220 ----------SDE-------ITIELDSLPPMVAKPFSPANGVSVEDVE--GTKIDQVFIG 260
SDE I ++ + P+VA P P N V + KIDQVFIG
Sbjct: 241 RAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQVFIG 300
Query: 261 SCTNGRFEQIKRAAEILDGEQVN--VRTLVAPASINVYRRMIEEGLVRILIDAGVVILPP 318
SCTNGR E ++ AAEIL+G++V VR +V P S VY + ++EGL+ I ++AG VI PP
Sbjct: 301 SCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPP 360
Query: 319 GCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNPE 377
CGPCLG HMGV E +STTNRNF GRMG PK+E+YLA+P AA SA+ G I +PE
Sbjct: 361 TCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPE 419
>dbj|BAA74762.1| (AB017109) HacA [Thermus thermophilus]
Length = 418
Score = 294 bits (745), Expect = 1e-78
Identities = 171/406 (42%), Positives = 234/406 (57%), Gaps = 30/406 (7%)
Query: 2 TLVGKLLNAK------PGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVR--TRAYIFFD 53
TL K+L+ K GE V+ +VD V D + + R R I D
Sbjct: 4 TLAEKILSHKVGRPVRAGELVVVEVDQVMVVDSIAGSFFKRLEYLEATPRYPERVSIVID 63
Query: 54 HVYPAPTVKVANLHREIREFAKIHGIPVVE-GKGISHQLVVELGLAEEAKIIVGGDSHTP 112
HV PA ++VA +EIRE+ K HGI V + G+G+ HQ+++E GLA+ ++VG DSH+
Sbjct: 64 HVAPAANLEVAKAQKEIREWGKRHGIRVFDVGRGVCHQVLIEEGLAQPGWVVVGSDSHST 123
Query: 113 TLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLK 172
T GA+G F GMGATD+A+A G+ WL+VPES+ V+ G + + A D + ++ L
Sbjct: 124 TYGAVGAFGTGMGATDIALAAASGRTWLRVPESVKVVFRGRLPKGVTAKDAALEMVRLLT 183
Query: 173 DREMSYNAIEFFNVP----FSFDERLTLTNFSVEANAKTAII---GEEYE---------- 215
+Y A+E + + ER+TL N +VEA AK ++ GE E
Sbjct: 184 AEGATYMAVEIHLLDGAEALTRGERMTLANLTVEAGAKAGLVVPSGEILEMYRVPDWLYP 243
Query: 216 --GGGYSDEITIELDSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRA 273
Y+ E+ I+L +L P V+ PF N V V+G ++DQVFIG+CTNGR E ++ A
Sbjct: 244 DPDARYAKEVEIDLSALTPRVSVPFYVDNVHEVAQVKGKRVDQVFIGTCTNGRIEDLRAA 303
Query: 274 AEILDGEQVN--VRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVA 331
AE+L G +V VR LV PAS V +G + L++AG I PGCGPC+GRHMGV
Sbjct: 304 AEVLRGRKVAPWVRLLVVPASSQVLEEAARDGTLLTLLEAGATIGTPGCGPCMGRHMGVL 363
Query: 332 GDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNPE 377
EV +ST+NRNFRGRMG+P AEIYLASP AA SA+ G +T PE
Sbjct: 364 APGEVCVSTSNRNFRGRMGAPDAEIYLASPRVAAASAVAGYLTTPE 409
>gi|11499545 aconitase (acn) [Archaeoglobus fulgidus]
>gi|3122334|sp|O28316|LE21_ARCFU PUTATIVE
3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7450351|pir||B69495 aconitase (acn) homolog -
Archaeoglobus fulgidus >gi|2648576|gb|AAB89290.1|
(AE000967) aconitase (acn) [Archaeoglobus fulgidus]
Length = 433
Score = 294 bits (744), Expect = 1e-78
Identities = 167/412 (40%), Positives = 235/412 (56%), Gaps = 38/412 (9%)
Query: 2 TLVGKLLN------AKPGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEV-----RTRAYI 50
TLV K+ + K GE V+ +DL HD T PL I+AF I +++ +
Sbjct: 19 TLVEKIFSKASGKEVKAGEFVMANIDLAMIHDITAPLAIKAFREILGSDAKVWDKSKVIM 78
Query: 51 FFDHVYPAPTVKVANLHREIREFAKIHGIPVVEGKG-ISHQLVVELGLAEEAKIIVGGDS 109
FDH PA +V A H+ +R+FA+ GI + KG I+HQ++VE E +IVG DS
Sbjct: 79 AFDHQVPADSVHAAENHKMLRKFAEEQGILNYDVKGGIAHQIMVE-NHVEPGMLIVGADS 137
Query: 110 HTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLIT 169
HT GALG FA G+G+TD+ L +GK+W KVPESI + G +++ D+++ LI
Sbjct: 138 HTCMYGALGAFATGIGSTDMGFVLAMGKLWFKVPESIRFNVHGKLEKHVYGKDIVLKLIG 197
Query: 170 SLKDREMSYNAIEFFNV---PFSFDERLTLTNFSVEANAKTAII-------------GEE 213
+ +Y A + +RLT+ N ++E K I+ G
Sbjct: 198 MVGADGANYKACIYSGEVVEKLGMSDRLTMCNMAIEMGGKAGIVEPDKTTLEYLKAMGRP 257
Query: 214 YEG-------GGYSDEITIELDSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGR 266
YEG E+ +++ + P VA P N V + +VEGT++DQVFIGSCTNGR
Sbjct: 258 YEGELLKSDEDAEFQEVELDVTGMEPQVAAPHRVDNVVGISEVEGTRVDQVFIGSCTNGR 317
Query: 267 FEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCL 324
+E +K AAEIL GE+V NVR +V PAS YRR ++EGL+ I +DAG ++ P CGPC+
Sbjct: 318 YEDLKIAAEILKGEKVASNVRLIVIPASHREYRRALKEGLIEIFVDAGALVEAPCCGPCM 377
Query: 325 GRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNP 376
G G+ EV +ST+NRNF GR GSP+ +IYL +P AA +A+YGEIT+P
Sbjct: 378 GGSFGLIASGEVSVSTSNRNFIGRQGSPEGKIYLVNPAVAAATAIYGEITDP 429
>sp|O27668|LE22_METTH PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7450349|pir||A69085 3-isopropylmalate dehydratase,
LeuC subunit - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2622758|gb|AAB86104.1| (AE000922)
3-isopropylmalate dehydratase, LeuC subunit
[Methanobacterium thermoautotrophicum]
Length = 428
Score = 286 bits (723), Expect = 4e-76
Identities = 160/407 (39%), Positives = 220/407 (53%), Gaps = 34/407 (8%)
Query: 7 LLNAKPGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRT-------RAYIFFDHVYPAP 59
L PGE + +VDL HDGT P I F I + R + FDH PA
Sbjct: 17 LREVTPGEIIEARVDLAMTHDGTSPPTIRTFRDIASRGGPARVWDPERIVMVFDHNVPAN 76
Query: 60 TVKVANLHREIREFAKIHGIPVV--EGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGAL 117
T+ A R REFA+ GI + GI HQ++ E G +IVG DSHT T GA
Sbjct: 77 TIGAAEFQRVTREFAREQGIVNIFQNAAGICHQVLPERGFVRPGMVIVGADSHTCTYGAF 136
Query: 118 GVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMS 177
G FA GMGATD+A+ GK W VPE++ + + G P ++ A DV++H+I + +
Sbjct: 137 GAFATGMGATDMAMVFATGKTWFMVPEAMRIEVTGEPEGHVYAKDVILHIIGEIGVDGAT 196
Query: 178 YNAIEFFN---VPFSFDERLTLTNFSVEANAKTAII--------------GEEY------ 214
Y ++EF R+T+ N +VE AK I+ G E+
Sbjct: 197 YRSVEFTGDTIESMDVSGRMTICNMAVEMGAKNGIMEPNRQTLDYVRARTGREFRVYSSD 256
Query: 215 EGGGYSDEITIELDSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAA 274
E Y ++ ++ L P VA P N V VEGT ID+ F+GSCTNGR+E +K AA
Sbjct: 257 EDSQYLEDHHFDVSDLEPQVACPDDVDNVYPVHRVEGTHIDEAFLGSCTNGRYEDLKIAA 316
Query: 275 EILDGEQVN--VRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAG 332
E++ +V+ VR +V+PAS +Y + +E+G++ I AG ++ PGCGPCLG HMGV
Sbjct: 317 EVIGDRRVHEDVRFIVSPASREIYLKALEDGIIETFIRAGAIVCNPGCGPCLGAHMGVLA 376
Query: 333 DNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNPEGE 379
EV ++TTNRNFRGRMG P + +YLA+P A SA+ G I+ P+ E
Sbjct: 377 PGEVSIATTNRNFRGRMGDPASSVYLANPAVVAESAIEGVISAPQQE 423
>gb|AAK42608.1| 3-isopropylmalate dehydratase, large subunit (isopropylmalate
isomerase) (alpha IPM isomerase) (IPMI) (leuC)
[Sulfolobus solfataricus]
Length = 416
Score = 283 bits (716), Expect = 3e-75
Identities = 161/411 (39%), Positives = 235/411 (57%), Gaps = 39/411 (9%)
Query: 2 TLVGKLLN------AKPGEAVIRKVDLVYAHDGTMPLIIEAFNR-----IFNEVRTRAYI 50
TL K+L+ PG+ + KVDLV HD T +IE + +F+ +++ I
Sbjct: 6 TLTEKILSRASGKDVSPGDVIEAKVDLVAFHDLTGYHVIEVMEKANMIKVFD--KSKLVI 63
Query: 51 FFDHVYPAPTVKVANLHREIREFAKIHGIPVVE--GKGISHQLVVELGLAEEAKIIVGGD 108
FDH+ P P V+ A + IR+F K GIP GI H++++E A ++I+ D
Sbjct: 64 AFDHLAPPPDVRSAEIQGYIRKFVKSEGIPNFYDINYGILHEVMIEQ-YANPGQVILAAD 122
Query: 109 SHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLI 168
SHT T GA+G FA GMGA+D+A AL GK WL VP+ V+L+G P ++I DV + L+
Sbjct: 123 SHTTTSGAVGAFAQGMGASDIAAALITGKTWLVVPQPFKVVLEGKPAKWITGKDVALKLL 182
Query: 169 TSLKDREMSYNAIEFF---NVPFSFDERLTLTNFSVEANAKTAIIGEEYE---------- 215
K + +E F + F D R T++N +E NA + + E
Sbjct: 183 GDFKADYFNGMTLEIFVKDPLSFPMDYRATVSNMGIEMNADALMFIPDQETKRYIKEMRG 242
Query: 216 ----------GGGYSDEITIELDSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNG 265
G Y DE TI+LD + P+VA P S N V ++EGT +DQV+IGSCTNG
Sbjct: 243 YEPELVTPDNGAKYVDEYTIQLDEMEPLVAAPHSVDNVKVVNELEGTPVDQVYIGSCTNG 302
Query: 266 RFEQIKRAAEILDGEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG 325
R + AA+I+ G++V R + PAS +++ +E G ++ L+DAG ++ CGPCLG
Sbjct: 303 RLSDFEIAAKIMKGKKVKSRCIAIPASYRMFKEALERGYIQTLVDAGCIVTYGTCGPCLG 362
Query: 326 RHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNP 376
H G+AG E I+ST++RNF+GRMGS +A++YL+ P AA+SAL G+IT+P
Sbjct: 363 GHFGIAGPGENIVSTSSRNFKGRMGSNEAKVYLSGPAVAAISALEGKITDP 413
>sp|O27439|LE21_METTH PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7450350|pir||D69051 3-isopropylmalate dehydratase,
LeuD subunit - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2622496|gb|AAB85863.1| (AE000901)
3-isopropylmalate dehydratase, LeuD subunit
[Methanobacterium thermoautotrophicum]
Length = 419
Score = 282 bits (714), Expect = 4e-75
Identities = 158/395 (40%), Positives = 229/395 (57%), Gaps = 32/395 (8%)
Query: 13 GEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRT----RAYIFFDHVYPAPTVKVANLHR 68
GE V+ +D+ HD T PL +E+F R E R + + FDH PA +++ A H
Sbjct: 22 GEIVMADIDVAMTHDLTGPLSVESF-RAIGEDRVWDPEKIVVIFDHQVPADSIEAAQNHM 80
Query: 69 EIREFAKIHGIPVVEG--KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGA 126
+R+F + GI +G+ HQ++ E G +++VG DSHT T GALG FA G+G+
Sbjct: 81 IMRDFVEEQGIRNFYDVREGVCHQVLPEKGHVVPGEVVVGTDSHTCTHGALGAFATGIGS 140
Query: 127 TDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFN- 185
TD+A+ GK+W +VPE++ + G RE++ A DV++++I + +Y A EF
Sbjct: 141 TDMAMVFATGKLWFRVPETLRFDVRGKLREHVYAKDVILNIIGRVGADGATYMACEFAGE 200
Query: 186 --VPFSFDERLTLTNFSVEANAKTAIIGEEYEGGGYSDE--------------------I 223
S +R+ L+N ++E KT I+ + + Y +
Sbjct: 201 TVAEMSVSDRMVLSNMAIEMGGKTGIVEPDEKTLNYVRRRSGKPWRVFKTDPDAPSLSVM 260
Query: 224 TIELDSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQVN 283
+++ L P VA P + N V +VEGT+IDQVF+GSCTNGR ++ AA IL +V+
Sbjct: 261 EVDVSDLEPQVACPHNVDNVKPVTEVEGTEIDQVFLGSCTNGRLSDLRDAAAILKNRKVS 320
Query: 284 --VRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTT 341
VR LV PAS VYRR ++EGL+ I +DAG ++ P CGPCLG H+G+ G EV LST+
Sbjct: 321 DSVRMLVIPASREVYRRALDEGLIEIFVDAGALVCNPCCGPCLGGHVGLVGPGEVSLSTS 380
Query: 342 NRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNP 376
NRNFRGR GSP+AE+YL+S AA SA+ G IT+P
Sbjct: 381 NRNFRGRQGSPEAEVYLSSAAVAAASAVKGSITHP 415
>pir||H72362 3-isopropylmalate dehydratase, large subunit - Thermotoga maritima
(strain MSB8) >gi|4981069|gb|AAD35639.1|AE001730_7
(AE001730) 3-isopropylmalate dehydratase, large subunit
[Thermotoga maritima]
Length = 417
Score = 282 bits (713), Expect = 6e-75
Identities = 169/414 (40%), Positives = 234/414 (55%), Gaps = 38/414 (9%)
Query: 1 MTLVGKLLNAK------PGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNE---VRTRAYIF 51
MTL K+L+ K PGE ++ + D+ A+D T PL I+ F + R +
Sbjct: 1 MTLAEKILSQKAGRKVEPGEFLLLEPDIALANDITAPLAIKKFKEYGGKKVKYPDRVVLV 60
Query: 52 FDHVYPAPTVKVANLHREIREFAKIHGIPV---VEGKGISHQLVVELGLAEEAKIIVGGD 108
DH P +K A + +REFA+ GI + GI H L+ E G+ + ++VG D
Sbjct: 61 PDHFTPNKDIKSAMQVKMMREFAREQGIEKFFEIGRMGIEHVLLPEEGIVKSGDLVVGAD 120
Query: 109 SHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLI 168
SHT T GALG FA G+G+TD+A +GK+W +VPESI V L G ++ + A D+++ LI
Sbjct: 121 SHTCTYGALGAFATGVGSTDIAGFYLIGKVWFRVPESIKVTLRGKFKDLVTAKDLVLKLI 180
Query: 169 TSLKDREMSYNAIEFFNV---PFSFDERLTLTNFSVEANAKTAI---------------- 209
+ L +Y AIEF S D R T++N ++EA KT +
Sbjct: 181 SILGVDGANYKAIEFSGPGVKEISMDGRFTISNMAIEAGGKTGLFPVDEITIAYERERGI 240
Query: 210 -IGEEY--EGGGYSDEITIELDSLPPMVAKPFSPANG--VSVEDVEGTKIDQVFIGSCTN 264
+ E Y E Y E+ ++L L P VA PF P+N VS + E KIDQ IGSCTN
Sbjct: 241 EVEEMYPDEDAKYVREVEMDLSELEPQVAYPFLPSNAKDVSEAEKERIKIDQAVIGSCTN 300
Query: 265 GRFEQIKRAAEILDGEQVN--VRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGP 322
GR E ++ AA+IL G V+ VR ++ P S VY++ ++EGL+ I IDAG + P CGP
Sbjct: 301 GRIEDLRLAAQILKGRTVSPDVRCIIIPGSQKVYKQALKEGLIDIFIDAGCAVSTPTCGP 360
Query: 323 CLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNP 376
CLG HMGV + EV +STTNRNF GRMG P ++++LASP AA SA+ G I +P
Sbjct: 361 CLGGHMGVLAEGEVAISTTNRNFVGRMGHPNSKVFLASPAVAAASAIKGYIADP 414
>pir||D75373 3-isopropylmalate dehydratase, large subunit - Deinococcus
radiodurans (strain R1)
>gi|6459378|gb|AAF11172.1|AE002005_1 (AE002005)
3-isopropylmalate dehydratase, large subunit
[Deinococcus radiodurans]
Length = 434
Score = 275 bits (696), Expect = 6e-73
Identities = 162/389 (41%), Positives = 219/389 (55%), Gaps = 24/389 (6%)
Query: 13 GEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRT---RAYIFFDHVYPAPTVKVANLHRE 69
G+ + +VD V D IE R V R I DHV PA TV VA +E
Sbjct: 37 GDLAVVEVDQVMVVDSIAQSFIERMERDLGAVPKYPERVSIVVDHVAPASTVSVAQAQKE 96
Query: 70 IREFAKIHGIPVVE-GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATD 128
RE+A G+ + + G+GI HQ+++E LA+ I++G DSH+ T GA+ F GMGATD
Sbjct: 97 AREYAAKTGVRLFDVGRGICHQVLMEEKLAQPGWIVLGSDSHSTTYGAVAAFGSGMGATD 156
Query: 129 VAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFF-NVP 187
+A+A GK WL+VPES+ V L G R + A DV + +I L +Y ++E
Sbjct: 157 IALAAASGKTWLRVPESVKVTLTGDLRPGVTAKDVALEMIRVLGADGATYQSVEIHAGDR 216
Query: 188 FSFDERLTLTNFSVEANAKTAII---GE---EY-----------EGGGYSDEITIELDSL 230
F+ ER+TL N VEA AK ++ GE +Y EG Y+ E+ I+L +L
Sbjct: 217 FTRGERMTLANLCVEAGAKAGLVVPGGEILTDYGYDVPAWVYPDEGAAYAREVEIDLSAL 276
Query: 231 PPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQVN--VRTLV 288
P ++ P N V ++ G K+DQVFIG+CTNGR E + AAE+L G +V+ R LV
Sbjct: 277 HPRMSAPSEVDNVHDVAELRGLKVDQVFIGTCTNGRIEDLHAAAEVLRGRRVDPTTRLLV 336
Query: 289 APASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGR 348
PAS V +++G + L AG V+ PGCGPC+GRH GV EV +ST+NRNF GR
Sbjct: 337 IPASSQVMEEALQDGTLLTLQRAGAVLGTPGCGPCMGRHQGVLAPGEVCVSTSNRNFIGR 396
Query: 349 MGSPKAEIYLASPVTAAVSALYGEITNPE 377
MG A IYLASP AA +A+ G + PE
Sbjct: 397 MGDKDAHIYLASPAVAAATAVMGRVALPE 425
>sp|Q58409|LE21_METJA PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|2127973|pir||B64425 homoaconitate hydratase (EC
4.2.1.36) - Methanococcus jannaschii
>gi|1591664|gb|AAB99007.1| (U67543) 3-isopropylmalate
dehydratase (leuC) [Methanococcus jannaschii]
Length = 420
Score = 270 bits (683), Expect = 2e-71
Identities = 163/419 (38%), Positives = 227/419 (53%), Gaps = 46/419 (10%)
Query: 1 MTLVGKLLNAK------PGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRT--RAYIFF 52
MTLV K+L+ K G+++ +VDL HDGT PL +A + + V + + F
Sbjct: 1 MTLVEKILSKKVGYEVCAGDSIEVEVDLAMTHDGTTPLAYKALKEMSDSVWNPDKIVVAF 60
Query: 53 DHVYPAPTVKVANLHREIREFAKIHGIPVVE--GKGISHQLVVELGLAEEAKIIVGGDSH 110
DH P TVK A + + EF K GI G+GI HQ++ E + + GGDSH
Sbjct: 61 DHNVPPNTVKAAEMQKLALEFVKRFGIKNFHKGGEGICHQILAENYVLPNM-FVAGGDSH 119
Query: 111 TPTLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITS 170
T T GA G FA G GATD+A G+ W+KVP++I V + G E + A D+++ +
Sbjct: 120 TCTHGAFGAFATGFGATDMAYIYATGETWIKVPKTIRVDIVGK-NENVSAKDIVLRVCKE 178
Query: 171 LKDREMSYNAIEFFN---VPFSFDERLTLTNFSVEANAKTAIIGEE-------------- 213
+ R +Y AIE+ D RLTL N ++E KT +I +
Sbjct: 179 IGRRGATYMAIEYGGEVVKNMDMDGRLTLCNMAIEMGGKTGVIEADEITYDYLKKERGLS 238
Query: 214 ---------------YEGGGYSDEITIELDSLPPMVAKPFSPANGVSVEDVEGTKIDQVF 258
+ Y EI I++ + VA P P N + DVEGT+I+QVF
Sbjct: 239 DEDIAKLKKERITVNRDEANYYKEIEIDITDMEEQVAVPHHPDNVKPISDVEGTEINQVF 298
Query: 259 IGSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVIL 316
IGSCTNGR ++ AA+ L G +V +V+ +V PAS V+ + ++EG++ I + AG +I
Sbjct: 299 IGSCTNGRLSDLREAAKYLKGREVHKDVKLIVIPASKKVFLQALKEGIIDIFVKAGAMIC 358
Query: 317 PPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITN 375
PGCGPCLG H GV + E+ LSTTNRNF+GRMG + IYLASP AA+SA+ G ITN
Sbjct: 359 TPGCGPCLGAHQGVLAEGEICLSTTNRNFKGRMGHINSYIYLASPKIAAISAVKGYITN 417
>sp|O67078|LEU2_AQUAE 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7450352|pir||C70381 large subunit of isopropylmalate
isomerase - Aquifex aeolicus >gi|2983447|gb|AAC07028.1|
(AE000714) large subunit of isopropylmalate isomerase
[Aquifex aeolicus]
Length = 432
Score = 268 bits (679), Expect = 6e-71
Identities = 154/421 (36%), Positives = 231/421 (54%), Gaps = 46/421 (10%)
Query: 1 MTLVGKLL-------NAKPGEAVIRKVDLVYAHDGTMPLIIE-----AFNRIFNEVRTRA 48
MT+ K+L PGE + K+DL A+D T PL I+ +++F+ R
Sbjct: 3 MTITEKILAEHAGKKEVHPGELITAKIDLAMANDVTAPLAIKILEKYGIDKVFDPERIA- 61
Query: 49 YIFFDHVYPAPTVKVANLHREIREFAKIHGIP--VVEGKGISHQLVVELGLAEEAKIIVG 106
+ H PA +K A + +REF K HGI EG+GI H ++ E GL ++VG
Sbjct: 62 -LVLSHFVPAKDIKSAEQAKIVREFVKKHGIKWFFEEGEGIEHAILPEQGLVVPGDLVVG 120
Query: 107 GDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIH 166
DSHT T GALG FA G+G+TD+A A+ G++WL+VPES+ + G + ++M D++++
Sbjct: 121 ADSHTCTYGALGAFATGVGSTDIAYAMATGEVWLRVPESMKFIFYGKLKPWVMGKDLILY 180
Query: 167 LITSLKDREMSYNAIEFFNVPF---SFDERLTLTNFSVEANAKTAIIGEEYE-------- 215
I + Y A+EF S ++R T+ N ++EA K+ II + +
Sbjct: 181 TIGQIGVDGALYRAMEFDGETIRNLSMEQRFTIANMAIEAGGKSGIISPDEKTIEYVKQR 240
Query: 216 ------------GGGYSDEITIELDSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCT 263
Y + + P+VA P+ P+N V + IDQ FIGSCT
Sbjct: 241 AKRPWKVYQSDPDAEYHSVYEWDASQIEPLVAWPYLPSNVHPVSESTHITIDQAFIGSCT 300
Query: 264 NGRFEQIKRAAEILD-----GEQVN--VRTLVAPASINVYRRMIEEGLVRILIDAGVVIL 316
NGR E ++ AA++ + G++V+ VR +V PAS +Y++ ++EGL+ I ++AG V+
Sbjct: 301 NGRIEDLRVAAKVFEAALKQGKKVHPYVRCIVIPASKAIYKQALKEGLIDIFMEAGCVVS 360
Query: 317 PPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNP 376
CGPCLG HMGV + E +ST+NRNF GRMG PK+E YLA+P A SA+ G I +P
Sbjct: 361 TSTCGPCLGGHMGVLAEGERCISTSNRNFPGRMGHPKSESYLANPAVVAASAIMGRIAHP 420
Query: 377 E 377
+
Sbjct: 421 D 421
>sp|P81291|LE22_METJA PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|2127740|pir||C64362 aconitate hydratase (EC 4.2.1.3)
- Methanococcus jannaschii >gi|1591201|gb|AAB98487.1|
(U67499) 3-isopropylmalate dehydratase (leuC)
[Methanococcus jannaschii]
Length = 424
Score = 253 bits (639), Expect = 3e-66
Identities = 150/421 (35%), Positives = 221/421 (51%), Gaps = 47/421 (11%)
Query: 1 MTLVGKLL-------NAKPGEAVIRKVDLVYAHDGTMPLIIEAFN-----RIFNEVRTRA 48
MT+V K+L PG+ V+ +D+ HD T PL + +++N +
Sbjct: 3 MTIVEKILAKASGKKEVSPGDIVMANIDVAMVHDITGPLTVNTLKEYGIEKVWNP--EKI 60
Query: 49 YIFFDHVYPAPTVKVANLHREIREFAKIHGIPVVEG--KGISHQLVVELGLAEEAKIIVG 106
I FDH PA ++K A H +R+F K GI +G+ HQ++ E G +++VG
Sbjct: 61 VILFDHQVPADSIKAAENHILMRKFVKEQGIKYFYDIREGVCHQVLPEKGHVAPGEVVVG 120
Query: 107 GDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIH 166
DSHT T GA G FA G+G+TD+A GK+W KVPE+I + G + Y+ + DV++
Sbjct: 121 ADSHTCTHGAFGAFATGIGSTDMAHVFATGKLWFKVPETIYFNITGDLQPYVTSKDVILS 180
Query: 167 LITSLKDREMSYNAIEFFNVP---FSFDERLTLTNFSVEANAKTAIIGEEY--------- 214
+I + +Y A +F S R+T+TN ++E KT II +
Sbjct: 181 IIGEVGVDGATYKACQFGGETVKKMSIASRMTMTNMAIEMGGKTGIIEPDEKTIQYVKEA 240
Query: 215 ----------------EGGGYSDEITIELDSLPPMVAKPFSPANGVSVEDVEGTKIDQVF 258
E +++ IE D + P+ A P + N +V G IDQVF
Sbjct: 241 MKKHGTERPFEVIKGDEDAEFAEVYEIEADKIEPVFACPHNVDNVKQAREVAGKPIDQVF 300
Query: 259 IGSCTNGRFEQIKRAAEILD---GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVI 315
IGSCTNGR E ++ A +I++ G +VR +V PAS Y + ++EG++ + G V+
Sbjct: 301 IGSCTNGRLEDLRMAIKIIEKHGGIADDVRVVVTPASREEYLKALKEGIIEKFLKYGCVV 360
Query: 316 LPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITN 375
P C C+G GV G EV +ST+NRNFRGR GS +AEIYLASP+TAA A+ GE+ +
Sbjct: 361 TNPSCSACMGSLYGVLGPGEVCVSTSNRNFRGRQGSLEAEIYLASPITAAACAVKGELVD 420
Query: 376 P 376
P
Sbjct: 421 P 421
>pir||T51171 homoaconitate hydratase (EC 4.2.1.36) [validated] - Thermus
aquaticus (fragment) >gi|3724368|dbj|BAA33786.1|
(AB018379) homoaconitate hydratase [Thermus
thermophilus]
Length = 386
Score = 238 bits (600), Expect = 1e-61
Identities = 146/381 (38%), Positives = 202/381 (52%), Gaps = 26/381 (6%)
Query: 2 TLVGKLLNAK------PGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVR--TRAYIFFD 53
TL K+L+ K GE V+ +VD V D + + R R I D
Sbjct: 6 TLAEKILSHKVGRPVRAGELVVVEVDQVMVVDSIAGSFFKRLEYLEATPRYPERVSIVID 65
Query: 54 HVYPAPTVKVANLHREIREFAKIHGIPVVE-GKGISHQLVVELGLAEEAKIIVGGDSHTP 112
HV PA ++VA +EIRE+ K HGI V + G+G+ HQ+++E GLA+ ++VG DSH+
Sbjct: 66 HVAPAANLEVAKAQKEIREWGKRHGIRVFDVGRGVCHQVLIEEGLAQPGWVVVGSDSHST 125
Query: 113 TLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLK 172
T GA+G F GMGATD+A+A G+ WL+VPES+ V+ G + + L
Sbjct: 126 TYGAVGAFGTGMGATDIALAAASGRTWLRVPESVKVVFRGRLPKGVHGQGRPPWRWVPLL 185
Query: 173 DREMSYNAIEFFNV----PFSFDERLTLTNFSVEANAKTAIIGEEYEGGG---------- 218
R + + +R+T A +G + G
Sbjct: 186 HRPEGATTWRWRSTLRRATLQRGQRMTSQLTVERAKRARVALGRSWRCTGARLALSRPRC 245
Query: 219 -YSDEITIELDSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEIL 277
Y+ E+ I+L +L P V+ PF N V V+G ++DQVFIG+CTNGR E ++ AAE+L
Sbjct: 246 PYAKEVEIDLSALTPRVSVPFYVDNVHEVAQVKGKRVDQVFIGTCTNGRIEDLRAAAEVL 305
Query: 278 DGEQVN--VRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNE 335
G +V VR LV PAS V +G + L++AG I PGCGPC+GRHMGV E
Sbjct: 306 RGRKVAPWVRLLVVPASSQVLEEAARDGTLLTLLEAGATIGTPGCGPCMGRHMGVLAPGE 365
Query: 336 VILSTTNRNFRGRMGSPKAEI 356
V +ST+NRNFRGRMG+P AEI
Sbjct: 366 VCVSTSNRNFRGRMGAPDAEI 386
>pir||H75354 3-isopropylmalate dehydratase, large subunit - Deinococcus
radiodurans (strain R1)
>gi|6459551|gb|AAF11331.1|AE002019_4 (AE002019)
3-isopropylmalate dehydratase, large subunit
[Deinococcus radiodurans]
Length = 431
Score = 231 bits (582), Expect = 1e-59
Identities = 137/418 (32%), Positives = 222/418 (52%), Gaps = 45/418 (10%)
Query: 1 MTLVGKLLNAK-------PGEAVIRKVDLVYAHDGTMPLIIE-----AFNRIFNEVRTRA 48
MT+ K+L A PG+ + D V H+ T P + +R+F+ + A
Sbjct: 3 MTIAEKMLAAHSGHDTVVPGQLIECATDWVLCHEITTPAALRMLEERGMDRVFDPQKIVA 62
Query: 49 YIFFDHVYPAPTVKVANLHREIREFAKIHGIPV---VEGKGISHQLVVELGLAEEAKIIV 105
DH PA +K A ++++++ + + GI V GI+H ++ GL + + +V
Sbjct: 63 VP--DHSVPAMNIKAAKMYQKLKSWVQEKGIEHFYDVGRGGIAHVVLENTGLMKPGQTLV 120
Query: 106 GGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMI 165
GDSHT GALG FA G+G+TD+A A+ GK+W KVPE++ + + G + D+++
Sbjct: 121 SGDSHTCNAGALGAFATGVGSTDLAGAIYAGKVWFKVPETMLIRVTGKTNPGVTPKDIVL 180
Query: 166 HLITSLKDREMSYNAIEF---FNVPFSFDERLTLTNFSVEANAKTAIIGEEYEGGGYSDE 222
+I + +Y +E+ + + R TLTN ++EA KT I+ + Y +
Sbjct: 181 EVIKQIGADGANYMVMEWVGDYIDQLDMEGRFTLTNMAIEAGGKTGIVAVDDTTRAYMQQ 240
Query: 223 --------------------ITIELDS--LPPMVAKPFSPANGVSVEDVEGTKIDQVFIG 260
+ +E+D+ + P VA P P+NG V + ++ ++G
Sbjct: 241 RGVSPEQYTEYQSDPDAKYKVVVEIDAAQVEPTVAYPHIPSNG-RVAGSDRIRVTHAYVG 299
Query: 261 SCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPP 318
SCTNGR ++ A IL G +V +V+ +V PA+ ++++ +EGL+ I +DAG + P
Sbjct: 300 SCTNGRITDLRDVARILKGRKVAQDVQMIVVPATQAIWKQAAQEGLLEIFVDAGASVSYP 359
Query: 319 GCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNP 376
CG CLG H GV G +++ +S++NRNF GRMG P A+IYLASP T A SA+ G I +P
Sbjct: 360 SCGACLGMHSGVLGPDDICISSSNRNFVGRMGDPSAQIYLASPATVAASAVEGFIADP 417
>pir||F70453 aconitase - Aquifex aeolicus >gi|2984078|gb|AAC07617.1| (AE000756)
aconitase [Aquifex aeolicus]
Length = 659
Score = 213 bits (536), Expect = 3e-54
Identities = 140/396 (35%), Positives = 205/396 (51%), Gaps = 33/396 (8%)
Query: 11 KPGEAVIRKVDLVYAHDGTMPLIIEAFNRI-FNEVRTRAYI-FFDHVYPAPTVKVANLHR 68
+PGE + K+D D T + F + EV+T + + DH + A+ H+
Sbjct: 21 EPGEEIAIKIDQTLTQDATGTMCYLEFEAMGVPEVKTELSVSYIDHNMLQTDFRNADDHK 80
Query: 69 EIREFAKIHGIPVVE-GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGAT 127
+ AK GI + + G GI HQ+ +E A+ K ++G DSHTPT G +G+ A+G G
Sbjct: 81 YLMSVAKKFGIWLSKPGNGICHQVHLER-FAKPGKTLLGSDSHTPTAGGMGMLAIGAGGL 139
Query: 128 DVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV- 186
DVA A+ +LK+P+ + V L G E++ A D+++ L+ L + E+F
Sbjct: 140 DVAAAMAGEPFYLKMPKIVGVHLKGKMPEWVTAKDIILELLRRLTVKGGLGKIFEYFGEG 199
Query: 187 --PFSFDERLTLTNFSVEANAKTAII-------------GEEYE--------GGGYSDEI 223
S ER T+TN E A T+I G E + Y + I
Sbjct: 200 VKELSVPERATITNMGAELGATTSIFPSDEITRAYLRAQGREEDWVELLPDPDAEYDEVI 259
Query: 224 TIELDSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQV- 282
I L L P++A+P SP N V V ++EG K+DQV IGSCTN F + RAA++L+G +V
Sbjct: 260 EINLSELEPLIAQPHSPDNVVPVREIEGIKVDQVVIGSCTNSSFVDLTRAAKLLEGRRVH 319
Query: 283 -NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVA-GDNEVILST 340
+V VAP S + + G + + AG IL CGPC+G MG A N V + +
Sbjct: 320 PDVVFAVAPGSKQALELITQNGALLNFLKAGARILESACGPCIG--MGFAPPTNGVSVRS 377
Query: 341 TNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNP 376
NRNF+GR G+P A++YLASP T +A+ GEI +P
Sbjct: 378 FNRNFKGRAGTPDAKVYLASPETCVAAAIAGEIIDP 413
>gb|AAG20620.1| (AE005132) aconitase; Can [Halobacterium sp. NRC-1]
Length = 661
Score = 195 bits (491), Expect = 6e-49
Identities = 135/399 (33%), Positives = 201/399 (49%), Gaps = 39/399 (9%)
Query: 11 KPGEAVIRKVDLVYAHDGTMPLIIEAFNRI-FNEVRTR-AYIFFDHVYPAPTVKVANLHR 68
+PGE + ++D + D T ++ F + +EV+T A + DH K + HR
Sbjct: 20 QPGEEIGIEIDQTLSQDTTGTMVWLQFEALEMDEVQTELAAQYCDHQTYQFDFKNTDDHR 79
Query: 69 EIREFAKIHGIPVVE-GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGAT 127
+R A +G G GI H + E A K ++G DSHTPT G LG A+G G
Sbjct: 80 FLRSAAGTYGAYFSRPGNGICHNVHKE-NFAAPGKTLLGSDSHTPTPGGLGQLAIGAGGI 138
Query: 128 DVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSL-------KDREMSYNA 180
D+A+A+G G ++++PE + V L+G E+ A DV++ L+ L K E +
Sbjct: 139 DIAVAMGGGAYYVEMPEVVNVRLEGELPEWATAKDVILELLRRLSVKGGVGKVLEYTGPG 198
Query: 181 IEFFNVPFSFDERLTLTNFSVEANAKTAII---------------GEEY------EGGGY 219
+E +VP ER T+TN E A ++I +E+ Y
Sbjct: 199 VESLSVP----ERTTITNMGTELGATSSIFPTDDRTEDWLARFDREDEFVDLQADADAEY 254
Query: 220 SDEITIELDSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEILDG 279
+DEI ++L L P++A+P P N V V +V G +++QV +GSCTNG +E + AA++L+G
Sbjct: 255 ADEIVVDLSDLEPLIAEPSMPDNVVPVREVAGQEVEQVMVGSCTNGAYEDVLPAAKMLEG 314
Query: 280 EQVNVRT--LVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVI 337
+V+ T +VAP S + EG ++ AGV CG C+G A D+ V
Sbjct: 315 REVSKHTEMIVAPGSKQASEMLAREGWTAEMMAAGVNFSEATCGACIGIGHVPASDS-VS 373
Query: 338 LSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNP 376
L T NRNF GR G +YL SP A SA+ GEI +P
Sbjct: 374 LRTFNRNFEGRSGIEDDSVYLCSPEVATASAIAGEIVDP 412
>pir||T06300 hypothetical protein T9E8.170 - Arabidopsis thaliana
>gi|4584548|emb|CAB40778.1| (AL049608) putative protein
[Arabidopsis thaliana] >gi|7268046|emb|CAB78385.1|
(AL161536) putative protein [Arabidopsis thaliana]
Length = 509
Score = 189 bits (475), Expect = 5e-47
Identities = 137/421 (32%), Positives = 196/421 (46%), Gaps = 57/421 (13%)
Query: 12 PGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRT-----RAYIFFDHVYPAPTVKVANL 66
PG+ + VD++ HD P F R F E + + DH Y K AN
Sbjct: 85 PGDNIWVNVDVLMTHDVCGPGAFGIFKREFGEKAKVWDPEKIVVIPDH-YIFTADKRANR 143
Query: 67 HREI-REFAKIHGIPVV-------------EGKGISHQLVVELGLAEEAKIIVGGDSHTP 112
+ +I RE + I + KG+ H + + G ++++G DSHT
Sbjct: 144 NVDIMREHCREQNIKYFYDITDLGNFKANPDYKGVCHVALAQEGHCRPGEVLLGTDSHTC 203
Query: 113 TLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLK 172
T GA G FA G+G TD LG GKI LKVP ++ +LDG Y+ A D+++ +I +
Sbjct: 204 TAGAFGQFATGIGNTDAGFVLGTGKILLKVPPTMRFILDGEMPSYLQAKDLILQIIGEIS 263
Query: 173 DREMSYNAIEFFNV---PFSFDERLTLTNFSVEANAKTAIIGEE---------------- 213
+Y +EF S +ER+TL N VEA K +I +
Sbjct: 264 VAGATYKTMEFSGTTIESLSMEERMTLCNMVVEAGGKNGVIPPDATTLNYVEACILSCFL 323
Query: 214 --YEGGGYS--DEITIELDSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFEQ 269
Y G S + ++ L P+VAKP SP N + + KID+V+IGSCT G+ E
Sbjct: 324 PVYSDGNASFVADYRFDVSKLEPVVAKPHSPDNRALARECKDVKIDRVYIGSCTGGKTED 383
Query: 270 IKRAAEILD--GEQVNVRTLVAPASINVYRRMIE--------EGLVRILIDAGV-VILPP 318
AA++ G +V V T + PA+ V+ + + +I +AG P
Sbjct: 384 FMAAAKLFHAAGRKVKVPTFLVPATQKVWMDVYALPVPGAGGKTCAQIFEEAGCDTPASP 443
Query: 319 GCGPCLGRHMGV---AGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITN 375
CG CLG + +V +STTNRNF GRMG + +IYLASP TAA SAL G + +
Sbjct: 444 SCGACLGGPADTYARLNEPQVCVSTTNRNFPGRMGHKEGQIYLASPYTAAASALTGRVAD 503
Query: 376 P 376
P
Sbjct: 504 P 504
>pir||T38665 probable homoaconitate hydratase (EC 4.2.1.36) - fission yeast
(Schizosaccharomyces pombe) >gi|5706517|emb|CAB52279.1|
(AL109739) putative homoaconitate hydratase (EC
4.2.1.36) [Schizosaccharomyces pombe]
Length = 721
Score = 174 bits (436), Expect = 2e-42
Identities = 127/414 (30%), Positives = 189/414 (44%), Gaps = 46/414 (11%)
Query: 11 KPGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRTRAYIFFDHVYPAPTVKVANL--HR 68
+ G+ V K +HD + P+ ++ +V I + ANL ++
Sbjct: 82 RSGDYVTIKPKHCMSHDNSWPVALKFMGIGAKKVFDNRQIVCTLDHDVQNKSEANLRKYK 141
Query: 69 EIREFAKIHGIPVVE-GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGAT 127
I FAK GI G+GI HQ++VE G A + V DSH+ T G +G + T
Sbjct: 142 NIESFAKGQGIDFYPAGRGIGHQIMVEQGYAMPGSMAVASDSHSNTYGGVGCLGTPIVRT 201
Query: 128 DVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV- 186
D A G+ W ++P V L G + + D+++ L + E+ +AIEF+
Sbjct: 202 DAAAIWATGQTWWQIPPIARVNLVGQLPKGLSGKDIIVSLCGAFNHDEVLNHAIEFYGEG 261
Query: 187 --PFSFDERLTLTNFSVEANAKTAI-----------------IGEEY------------- 214
S + RLT+ N + E A + + +G +
Sbjct: 262 LNSLSIESRLTIANMTTEWGALSGLFPTDEKLLAWYEDRLKFLGPNHPRVNRETLDAIKA 321
Query: 215 ------EGGGYSDEITIELDSLPPMVAKPFSPA--NGVSVEDVEGTKIDQVFIGSCTNGR 266
EG Y+ + ++L +L P V+ P S N + + + I + ++ SCTNGR
Sbjct: 322 SPILADEGAFYAKHLILDLSTLSPAVSGPNSVKVYNSAATLEKKDILIKKAYLVSCTNGR 381
Query: 267 FEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCL 324
I AAE + G++V V V AS V + G + LID+G LP GCGPC+
Sbjct: 382 LSDIHDAAETVKGKKVADGVEFYVGAASSEVEAAAQKNGDWQTLIDSGARTLPAGCGPCI 441
Query: 325 GRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNPEG 378
G G+ D EV +S TNRNF+GRMGS +A YLASP A SA+ G+I PEG
Sbjct: 442 GLGTGLLKDGEVGISATNRNFKGRMGSREALAYLASPAVVAASAIAGKIVAPEG 495
>sp|P80858|LEU2_BACSU 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
(SUPEROXIDE-INDUCIBLE PROTEIN 10) (SOI10)
>gi|7450355|pir||B69650 3-isopropylmalate dehydratase
(EC 4.2.1.33) large chain - Bacillus subtilis
>gi|1770071|emb|CAA99533.1| (Z75208) 3-isopropylmalate
dehydratase, large subunit [Bacillus subtilis]
>gi|2635291|emb|CAB14786.1| (Z99118) 3-isopropylmalate
dehydratase (large subunit) [Bacillus subtilis]
Length = 472
Score = 164 bits (411), Expect = 2e-39
Identities = 129/446 (28%), Positives = 201/446 (44%), Gaps = 88/446 (19%)
Query: 13 GEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRTRAYIFFDHVYPAPTV----------- 61
G+ + +DL H+ T P E + +VR F + PTV
Sbjct: 21 GKPDLLYIDLHLIHEVTSPQAFEGLRQKGRKVRRPQNTFATMDHNIPTVNRFEIKDEVAK 80
Query: 62 -KVANLHREIREF----AKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGA 116
+V L R EF A +H + +GI H + ELGL K IV GDSHT T GA
Sbjct: 81 RQVTALERNCEEFGVRLADLHSVD----QGIVHVVGPELGLTLPGKTIVCGDSHTSTHGA 136
Query: 117 LGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREM 176
G A G+G ++V L +W + P+++ V +DG+ ++ + A DV++ +I +
Sbjct: 137 FGALAFGIGTSEVEHVLSTQTLWQQRPKTLEVRVDGTLQKGVTAKDVILAVIGKYGVKFG 196
Query: 177 SYNAIEFFNVPF---SFDERLTLTNFSVEANAKTAII------------------GEEYE 215
+ IE+ F + DER+T+ N S+EA A+ +I GEE++
Sbjct: 197 TGYVIEYTGEVFRNMTMDERMTVCNMSIEAGARAGLIAPDEVTFEYCKNRKYTPKGEEFD 256
Query: 216 -------------GGGYSDEITIELDSLPPMVAKPFSPA------------NGVSVED-- 248
G Y I ++ + + PMV +P S ED
Sbjct: 257 KAVEEWKALRTDPGAVYDKSIVLDGNKISPMVTWGINPGMVLPVDSEVPAPESFSAEDDK 316
Query: 249 -----------------VEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVRTLVA 289
+E K++ VFIGSCTN R +++AA+++ G++V +VR +V
Sbjct: 317 KEAIRAYEYMGLTPHQKIEDIKVEHVFIGSCTNSRMTDLRQAADMIKGKKVADSVRAIVV 376
Query: 290 PASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRM 349
P S +V + +EGL +I ++AG GC CL + V + E ST+NRNF GR
Sbjct: 377 PGSQSVKLQAEKEGLDQIFLEAGFEWRESGCSMCLSMNNDVVPEGERCASTSNRNFEGRQ 436
Query: 350 GSPKAEIYLASPVTAAVSALYGEITN 375
G A +L SP AA++A++G +
Sbjct: 437 GK-GARTHLVSPAMAAMAAIHGHFVD 461
>gi|6320440 homoaconitase; Lys4p [Saccharomyces cerevisiae]
>gi|1346476|sp|P49367|LYS4_YEAST HOMOACONITASE PRECURSOR
(HOMOACONITATE HYDRATASE) >gi|2131287|pir||S61067
homoaconitate hydratase (EC 4.2.1.36) precursor - yeast
(Saccharomyces cerevisiae) >gi|1089840|emb|CAA63764.1|
(X93502) homoaconitase [Saccharomyces cerevisiae]
>gi|1197581|gb|AAA88902.1| (U46154) lysine biosynthesis
enzyme homoaconitase [Saccharomyces cerevisiae]
Length = 693
Score = 158 bits (396), Expect = 9e-38
Identities = 124/428 (28%), Positives = 197/428 (45%), Gaps = 58/428 (13%)
Query: 5 GKLLNAKPGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRTRAYIF--FDHVYPAPTVK 62
GK++++ G+ V K +HD + P+ ++ +++ + I DH + K
Sbjct: 38 GKVVHS--GDYVSIKPAHCMSHDNSWPVALKFMGLGATKIKNPSQIVTTLDHDIQNKSEK 95
Query: 63 VANLHREIREFAKIHGIP-VVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFA 121
++ I FAK H I G+GI HQ+++E G A + V DSH+ T G LG
Sbjct: 96 NLTKYKNIENFAKKHHIDHYPAGRGIGHQIMIEEGYAFPLNMTVASDSHSNTYGGLGSLG 155
Query: 122 VGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAI 181
+ TD A G+ W ++P V L G + + D+++ L + ++ +AI
Sbjct: 156 TPIVRTDAAAIWATGQTWWQIPPVAQVELKGQLPQGVSGKDIIVALCGLFNNDQVLNHAI 215
Query: 182 EFFNVPFS---FDERLTLTNFSVE--------------------------------ANAK 206
EF + D RLT+ N + E N K
Sbjct: 216 EFTGDSLNALPIDHRLTIANMTTEWGALSGLFPVDKTLIDWYKNRLQKLGTNNHPRINPK 275
Query: 207 T-------AIIGEEYEGGGYSDEITIELDSLPPMVAKPFSPANGVSVEDV--EGTKIDQV 257
T A I + + Y+ ++ I+L +L V+ P S +V+D+ + KI++
Sbjct: 276 TIRALEEKAKIPKADKDAHYAKKLIIDLATLTHYVSGPNSVKVSNTVQDLSQQDIKINKA 335
Query: 258 FIGSCTNGRFEQIKRAAEIL----DGEQVN-----VRTLVAPASINVYRRMIEEGLVRIL 308
++ SCTN R ++ AA+++ D +VN V VA AS + + G L
Sbjct: 336 YLVSCTNSRLSDLQSAADVVCPTGDLNKVNKVAPGVEFYVAAASSEIEADARKSGAWEKL 395
Query: 309 IDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSA 368
+ AG + LP GCGPC+G G+ EV +S TNRNF+GRMGS A YLASP A SA
Sbjct: 396 LKAGCIPLPSGCGPCIGLGAGLLEPGEVGISATNRNFKGRMGSKDALAYLASPAVVAASA 455
Query: 369 LYGEITNP 376
+ G+I++P
Sbjct: 456 VLGKISSP 463
>sp|Q92412|LYS4_EMENI HOMOACONITASE PRECURSOR (HOMOACONITATE HYDRATASE)
>gi|1628374|emb|CAA67943.1| (X99624) homoaconitase
[Aspergillus nidulellus]
Length = 775
Score = 157 bits (394), Expect = 1e-37
Identities = 123/434 (28%), Positives = 190/434 (43%), Gaps = 70/434 (16%)
Query: 11 KPGEAVIRKVDLVYAHDGTMPLIIEAFN----RIFNEVRTRAYIFFDHVYPAPTVKVANL 66
K G+ V + HD + P ++ + R+ N + + DH + K
Sbjct: 87 KSGDYVTIQPHRCMTHDNSWPCALKFMSIGASRLHNP--DQIVMTLDHDVQNKSDKNLKK 144
Query: 67 HREIREFAKIHGIPVVE-GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMG 125
+R+I EFA HG+ G+GI HQ+++E G A + V DSH+ G +G +
Sbjct: 145 YRQIEEFATQHGVEFYPAGRGIGHQIMIEEGFAWPGTLAVASDSHSNMYGGVGCLGTPIV 204
Query: 126 ATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFN 185
TD A GK W ++P V G + DV++ L ++ +AIEF
Sbjct: 205 RTDAASVWATGKTWWQIPPVAKVTFKGVLPPGVTGKDVIVALCGLFNKDDVLNHAIEFTG 264
Query: 186 VP-----FSFDERLTLTNFSVE-------------------ANAKTAIIG---------- 211
S D RLT+ N + E A TA +G
Sbjct: 265 SEETMRSLSVDTRLTIANMTTEWGALSGLFPIDSVLKGWLRGKATTAAMGLADGPFKTRA 324
Query: 212 -EEY----------------EGGGYSDEITIELDSLPPMVAKPFSPANGVSVEDVEGT-- 252
E + +G Y+ E+ ++L SL P V+ P S ++++E
Sbjct: 325 AERFTHPLLEQLFENPLTADKGAKYAKELFLDLSSLSPYVSGPNSVKVATPLKELEAQNI 384
Query: 253 KIDQVFIGSCTNGRFEQIKRAAEIL------DGEQV----NVRTLVAPASINVYRRMIEE 302
K+D+ ++ SCTN R I AA++ +G ++ V+ +A ASI
Sbjct: 385 KVDKAYLVSCTNSRASDIAAAAKVFKEAAEKNGGKIPKIHGVKFYIAAASIPEQLAAEGN 444
Query: 303 GLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPV 362
G + L++AG LP GCGPC+G G+ EV +S +NRNF+GRMGS +A+ YL SP
Sbjct: 445 GDWQTLLEAGATQLPAGCGPCIGMGQGLLEPGEVGISASNRNFKGRMGSTEAKAYLGSPE 504
Query: 363 TAAVSALYGEITNP 376
A SAL G+++ P
Sbjct: 505 VVAASALSGKLSGP 518
>dbj|BAB06775.1| (AP001517) 3-isopropylmalate dehydratase large subunit [Bacillus
halodurans]
Length = 472
Score = 157 bits (392), Expect = 3e-37
Identities = 133/458 (29%), Positives = 189/458 (41%), Gaps = 85/458 (18%)
Query: 2 TLVGKLLNA-----KPGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRTRAYIFFDHVY 56
T++ K+ +A + G+ + +DL H+ T P E VR F +
Sbjct: 5 TIIEKIWDAHTVIGEEGKPSLLYIDLHMVHEVTSPQAFEGLRLAGRPVRRPDLTFATMDH 64
Query: 57 PAPTVKVANLHREIREF--------AKIHGIPVV----EGKGISHQLVVELGLAEEAKII 104
PTV N+ +I K GI + GI H + ELGL + K I
Sbjct: 65 NVPTVDRFNIQDQIARKQIETLEANCKEFGIEIAGLDSPNNGIVHVIGPELGLTQPGKTI 124
Query: 105 VGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVM 164
V GDSHT T GA G A G+G ++V L +W P+++ V + G + A D++
Sbjct: 125 VCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLWQSKPKTMEVRVTGELAPSVSAKDII 184
Query: 165 IHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNFSVEANAKTAII----------- 210
+ +I + + IEF S +ER+T+ N S+EA AK +I
Sbjct: 185 LAVIAKYGVDFGTGHVIEFTGEAIRSLSMEERMTICNMSIEAGAKAGLISPDSVTFEYLR 244
Query: 211 -------GEEYE-------------GGGYSDEITIELDSLPPMVAKPFSPANGVSVEDV- 249
GE ++ G Y +T+ + PMV +PA G V V
Sbjct: 245 GRPNAPKGEAFDVAIQQWEALATDAGAVYDRVLTMNASEIEPMVTWGTNPAQGTGVSQVV 304
Query: 250 -------------------------EGTKIDQ-----VFIGSCTNGRFEQIKRAAEILDG 279
GT I + VFIGSCTN R ++ AAE++ G
Sbjct: 305 PSPDDAKDENERRAIKQSLAYMGLEPGTPITEIAIQHVFIGSCTNSRLSDLRTAAELIKG 364
Query: 280 EQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVI 337
+V VR LV P S V R +EGL I +AG GC CLG + + E
Sbjct: 365 RKVADGVRALVVPGSQQVKRAAEKEGLDEIFKEAGFEWRDSGCSMCLGMNPDTVPEGERC 424
Query: 338 LSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITN 375
ST+NRNF GR G A +L SP AA +A+ G +
Sbjct: 425 ASTSNRNFEGRQGK-GARTHLVSPQMAAAAAIAGHFVD 461
>sp|O53237|LEU2_MYCTU 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7450356|pir||G70853 probable leuC protein -
Mycobacterium tuberculosis (strain H37RV)
>gi|2791585|emb|CAA16073.1| (AL021287) leuC
[Mycobacterium tuberculosis]
Length = 473
Score = 150 bits (376), Expect = 2e-35
Identities = 123/438 (28%), Positives = 190/438 (43%), Gaps = 83/438 (18%)
Query: 20 VDLVYAHDGTMPLIIEAFNRIFNEVRTRAYIFFDHVYPAPTV-------------KVANL 66
+DL H+ T P + VR + PTV +V L
Sbjct: 34 IDLHLVHEVTSPQAFDGLRLAGRRVRRPELTLATEDHNVPTVDIDQPIADPVSRTQVETL 93
Query: 67 HREIREFA-KIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMG 125
R EF ++H + +E +GI H + +LGL + IV GDSHT T GA G A+G+G
Sbjct: 94 RRNCAEFGIRLHSMGDIE-QGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGALAMGIG 152
Query: 126 ATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFN 185
++V L + L+ +++AV +DG + + A D+++ LI + + IE+
Sbjct: 153 TSEVEHVLATQTLPLRPFKTMAVNVDGRLPDGVSAKDIILALIAKIGTGGGQGHVIEYRG 212
Query: 186 VPF---SFDERLTLTNFSVEANAKTAII------------------GEEYE--------- 215
S + R+T+ N S+EA A+ ++ G +++
Sbjct: 213 SAIESLSMEGRMTICNMSIEAGARAGMVAPDETTYAFLRGRPHAPTGAQWDTALVYWQRL 272
Query: 216 ----GGGYSDEITIELDSLPPMVAKPFSPANGVSV-----------EDVE---------- 250
G + E+ ++ SL P V +P GV + +D E
Sbjct: 273 RTDVGAVFDTEVYLDAASLSPFVTWGTNPGQGVPLAAAVPDPQLMTDDAERQAAEKALAY 332
Query: 251 -----GTK-----IDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRR 298
GT +D VF+GSCTNGR E ++ AE+L G +V VR L+ P S+ V +
Sbjct: 333 MDLRPGTAMRDIAVDAVFVGSCTNGRIEDLRVVAEVLRGRKVADGVRMLIVPGSMRVRAQ 392
Query: 299 MIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYL 358
EGL I DAG GC CLG + E +T+NRNF GR G+ +L
Sbjct: 393 AEAEGLGEIFTDAGAQWRQAGCSMCLGMNPDQLASGERCAATSNRNFEGRQGA-GGRTHL 451
Query: 359 ASPVTAAVSALYGEITNP 376
SP AA +A+ G +++P
Sbjct: 452 VSPAVAAATAVRGTLSSP 469
>sp|O33123|LEU2_MYCLE 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|11280213|pir||T45425 3-isopropylmalate dehydratase
[imported] - Mycobacterium leprae
>gi|2414558|emb|CAB16447.1| (Z99263) 3-isopropylmalate
dehydratase [Mycobacterium leprae]
Length = 476
Score = 145 bits (363), Expect = 6e-34
Identities = 113/388 (29%), Positives = 174/388 (44%), Gaps = 70/388 (18%)
Query: 57 PAPTVKVANLHREIREFA-KIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLG 115
P ++V L R EF ++H + +E +GI H + +LGL + IV GDSHT T G
Sbjct: 87 PVSRIQVETLRRNCAEFGVRLHPMGDIE-QGIVHVVGPQLGLTQPGMTIVCGDSHTSTHG 145
Query: 116 ALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDRE 175
A G A+G+G +++ L + L+ +++AV +DG + A D+++ LI +
Sbjct: 146 AFGAIAMGIGTSEIEHVLATQTLPLRPFKTMAVTVDGRLPAGVTAKDIILALIAKIGTGG 205
Query: 176 MSYNAIEFFNV---PFSFDERLTLTNFSVEANAKTAII------------------GEEY 214
+E+ S + R+T+ N S+EA A+ ++ G+++
Sbjct: 206 GQGYVLEYRGSVIESMSMEGRMTICNMSIEAGARAGMVAPDETTYEFLRDRPHAPTGKQW 265
Query: 215 EGGG-------------YSDEITIELDSLPPMVAKPFSPANGV----SVEDVE------- 250
+ + E+ ++ SL P V +P GV SV D E
Sbjct: 266 DAAVAYWQQLRTDDDAVFDTEVYLDATSLSPFVTWGTNPGQGVPLAASVPDPELMIDDVA 325
Query: 251 ---------------GTK-----IDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVRTLV 288
GT +D VF+GSCTNGR E ++ A++L G +V VR L+
Sbjct: 326 RQAAEKALAYMDLRPGTPMRDIAVDAVFVGSCTNGRIEDLRLVADVLRGHKVADGVRMLI 385
Query: 289 APASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGR 348
P S+ V + EGL I I AG PGC CLG + E +T+NRNF GR
Sbjct: 386 VPGSMRVRAQAEAEGLGEIFITAGAQWRQPGCSMCLGMNPDQLAPGERCAATSNRNFEGR 445
Query: 349 MGSPKAEIYLASPVTAAVSALYGEITNP 376
G +L SP AA +A+ G + P
Sbjct: 446 QGK-GGRTHLVSPAVAAATAVRGTFSAP 472
>pir||A81270 3-isopropylmalate dehydratase (EC 4.2.1.33) large chain Cj1717c
[imported] - Campylobacter jejuni (strain NCTC 11168)
>gi|6969132|emb|CAB73703.1| (AL139079) 3-isopropylmalate
dehydratase large subunit [Campylobacter jejuni]
Length = 470
Score = 142 bits (355), Expect = 6e-33
Identities = 104/358 (29%), Positives = 162/358 (45%), Gaps = 68/358 (18%)
Query: 83 EGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKV 142
+ +GI H + ELG +V GDSHT T GA G A G+G ++V + +
Sbjct: 105 KNQGIVHIVGPELGFTLPGVTLVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLKQAK 164
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
+++ + G ++ + D++++LI + + AIEF S + R+TL N
Sbjct: 165 LKTMKIECKGQFQKGVYTKDLILYLIAQYGTAKGTGYAIEFCGELIRNLSMEARMTLCNM 224
Query: 200 SVEANAKTAII------------------GEEY-------------EGGGYSDEITIELD 228
++E AK +I GEE+ EG Y IT+++
Sbjct: 225 AIEFGAKVGMIAPDEITFEYIKGKEFAPKGEEFQKYCEYWKSLRSDEGAKYDASITLDVS 284
Query: 229 SLPPMVAKPFSPANGVSVED-------------------------------VEGTKIDQV 257
+ P ++ +P+ + +++ +EG KID V
Sbjct: 285 KIKPQISYGTNPSQVIGIDEKIPKISDFKNQSEQKSLLDALYYVNLEQDQVIEGVKIDIV 344
Query: 258 FIGSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVI 315
FIGSCTNGR E +K AA+IL G ++ NV+ L+ P S+ V + GL +I I+AG
Sbjct: 345 FIGSCTNGRLEDLKIAADILKGRKIHKNVKALIVPGSMQVRKEAENLGLDKIFIEAGCEW 404
Query: 316 LPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEI 373
GC CLG + A + + ST+NRNF GR G +L SP +AA A+ G I
Sbjct: 405 RYAGCSMCLGMNDDKANSGQRVASTSNRNFVGRQGKGSI-THLMSPASAAACAIEGVI 461
>sp|Q44427|LEU2_ACTTI 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|2127501|pir||I39699 3-isopropylmalate dehydratase
(EC 4.2.1.33) large chain - Actinoplanes teichomyceticus
>gi|1008942|emb|CAA59140.1| (X84647) large subunit of
isopropilmalate isomerase [Actinoplanes teichomyceticus]
Length = 485
Score = 141 bits (351), Expect = 2e-32
Identities = 101/359 (28%), Positives = 155/359 (43%), Gaps = 68/359 (18%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + +LGL + IV GDSHT T GA G A G+G ++V L + P+
Sbjct: 125 QGIVHVIGPQLGLTQPGMTIVCGDSHTATHGASGALAFGIGTSEVEHVLATQTLPQSKPK 184
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNVPF---SFDERLTLTNFSV 201
++AV + G R + A D+++ LIT + + +E+ S + R+T+ N S+
Sbjct: 185 TMAVTVVGELRPGVSAKDLILTLITQTGTGGGNGHIVEYRGEAIRKLSMEGRMTICNMSI 244
Query: 202 EANAKTAIIGEEY-------------------------------EGGGYSDEITIELDSL 230
E AK +I + EG Y EI ++ +
Sbjct: 245 EWGAKAGMIAPDETTFDYLSGRKHARRGADWDAAVAYWKTLATDEGAEYDTEIILDASKI 304
Query: 231 PPMVAKPFSPANGVSVEDV-------------------------------EGTKIDQVFI 259
P + +P G +++ V +D VF+
Sbjct: 305 SPFITWGTNPGQGAALDGVVPDPQDFLDEVERGAAERALAYMGLTPGTPFRDVPVDVVFV 364
Query: 260 GSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILP 317
GSCTNGR E ++ AA+++ G +V VR ++ P S V + EGL +I IDAG
Sbjct: 365 GSCTNGRLEDLRAAADVIRGRKVADGVRMMIVPGSYQVREQAEAEGLDKIFIDAGAEWRF 424
Query: 318 PGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNP 376
GC CLG + + ST+NRNF GR G +L SP AA +A+ G++ P
Sbjct: 425 AGCSMCLGMNPDTLSPGQRAASTSNRNFEGRQGK-GGRTHLVSPQVAAATAVVGKLAAP 482
>pir||T29083 3-isopropylmalate dehydratase (EC 4.2.1.33) large chain -
Streptomyces coelicolor >gi|3355701|emb|CAA20001.1|
(AL031124) 3-isopropylmalate dehydratase large subunit
[Streptomyces coelicolor A3(2)]
Length = 476
Score = 141 bits (351), Expect = 2e-32
Identities = 119/445 (26%), Positives = 183/445 (40%), Gaps = 83/445 (18%)
Query: 13 GEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRTRAYIFFDHVYPAPTVKV--------- 63
GE + +DL H+ T P + + VR + PT+ +
Sbjct: 20 GEPDLLFIDLHLLHEVTSPQAFDGLRKSGRPVRRLDLTIATEDHNTPTLDIDKPIADPVS 79
Query: 64 ----ANLHREIREFA-KIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALG 118
L + +F ++H + VE +G+ H + +LGL + +V GDSHT T GA G
Sbjct: 80 RAQLETLRKNCADFGVRLHPLGDVE-QGVVHVVGPQLGLTQPGTTVVCGDSHTSTHGAFG 138
Query: 119 VFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSY 178
A G+G + V L + L P+++A+ +DG E + A D+++ +I +
Sbjct: 139 ALAFGIGTSQVEHVLATQTLPLARPKTMAITVDGELPEGVTAKDLILAIIARIGTGGGQG 198
Query: 179 NAIEFFNVPF---SFDERLTLTNFSVEANAKTAIIGE----------------------- 212
+E+ S + R+T+ N S+EA A+ +I
Sbjct: 199 YILEYRGEAIEKLSMEARMTICNMSIEAGARAGMIAPDETTFAYLQGRPHAPEGADWDAA 258
Query: 213 -EY-------EGGGYSDEITIELDSLPPMVAKPFSPANGV----------SVED------ 248
EY + + E+ IE L P V +P G S ED
Sbjct: 259 VEYWKTLRTDDDAEFDAEVVIEAAELAPFVTWGTNPGQGAPLSAAVPDPASYEDASERFA 318
Query: 249 ---------------VEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPA 291
+ ++D VF+GSCTNGR E ++ AAEI+ +V VR LV P
Sbjct: 319 AEKALEYMGLEAGQPLRSIQVDTVFVGSCTNGRIEDLRAAAEIVRDRKVADGVRMLVVPG 378
Query: 292 SINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGS 351
S V + + EGL + +AG GC CLG + E ST+NRNF GR G
Sbjct: 379 SARVGLQAVSEGLDVVFKEAGAEWRHAGCSMCLGMNPDQLAPGERSASTSNRNFEGRQGK 438
Query: 352 PKAEIYLASPVTAAVSALYGEITNP 376
+L SP AA +A+ G + +P
Sbjct: 439 -GGRTHLVSPQVAAATAVLGHLASP 462
>gi|10954460 3-isopropylmalate isomerase subunit [Buchnera aphidicola]
>gi|3282742|gb|AAD12602.1| (AF041837) 3-isopropylmalate
isomerase subunit [Buchnera aphidicola]
Length = 466
Score = 140 bits (350), Expect = 2e-32
Identities = 104/359 (28%), Positives = 165/359 (44%), Gaps = 68/359 (18%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + E G+ +IV GDSHT T GA G + G+G ++V L + + +
Sbjct: 105 QGIVHVISPEQGMTLPGMVIVCGDSHTSTHGAFGALSFGIGTSEVEHVLATQTLKQQRFK 164
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFF-NV--PFSFDERLTLTNFSV 201
++ + + G +I A DV++++I + + IEF NV S +ER+T+ N ++
Sbjct: 165 NMKIEVIGKIGNFITAKDVILYIIGKIGSSAGTGYIIEFCGNVIKKMSMEERMTVCNMAI 224
Query: 202 EANAKTAIIGEE-----------YEGGG--------------------YSDEITIELDSL 230
E AK+ +I + Y G + TI++ ++
Sbjct: 225 ELGAKSGLIAPDETTYLYLKNKTYSPHGRNWQKAIEYWKTLKTDHNAIFDKVFTIDISNI 284
Query: 231 PPMVAKPFSPANGVSVED-------------------------------VEGTKIDQVFI 259
P V +P + + + + ID+VFI
Sbjct: 285 LPQVTWGTNPDQVIGINEKIPDFTSFQNIVKKDLAKSACKYMDLKPGTYLTDITIDKVFI 344
Query: 260 GSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILP 317
GSCTN R E ++ A++IL ++ NV+ +V P S V R+ EGL +I I+AG
Sbjct: 345 GSCTNSRIEDLRSASKILKHNKISKNVKAIVVPGSGLVKRQAESEGLDKIFIEAGFEWRL 404
Query: 318 PGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNP 376
PGC CLG + +NE ST+NRNF GR G +L SP+ AA++ALYG+ +NP
Sbjct: 405 PGCSMCLGMNNDRLSENERCASTSNRNFEGRQGR-NGRTHLVSPIMAALAALYGKFSNP 462
>gi|10954452 isopropylmalate isomerase subunit [Buchnera aphidicola]
>gi|3282734|gb|AAD12595.1| (AF041836) isopropylmalate
isomerase subunit [Buchnera aphidicola]
Length = 469
Score = 140 bits (350), Expect = 2e-32
Identities = 103/358 (28%), Positives = 161/358 (44%), Gaps = 69/358 (19%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + E GL IV GDSHT T GA G + G+G ++V L + + +
Sbjct: 105 QGIVHVIGPEQGLTLPGSTIVCGDSHTSTHGAFGALSFGIGTSEVEHVLVTQTLKQQRFK 164
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFN---VPFSFDERLTLTNFSV 201
++ + + G +++I A D+++ +I L S IEF + +ER+T+ N ++
Sbjct: 165 NMKIKIVGKVKKFITAKDIILFIIGKLGTSSGSGYVIEFCGDVIKKMNMEERMTICNMAI 224
Query: 202 EANAKTAIIGEEY-------------------------------EGGGYSDEITIELDSL 230
E AK+A+I + E + E T ++ +
Sbjct: 225 EMGAKSALIAPDETTYLYLKNKRYSPKNKYWDSAIRYWKTLVTDENALFDKEFTFDISDI 284
Query: 231 PPMVAKPFSPANGVSVED-------------------------------VEGTKIDQVFI 259
P V SP +S+ + ++ KID+VFI
Sbjct: 285 SPQVTWGTSPDQVLSINEKIPDFNSFKDSIKKDLARSACNYMDLKPGSYLKNIKIDKVFI 344
Query: 260 GSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILP 317
GSCTN R E ++ AA IL +++ N++ +V P S +V + +EGL +I IDAG
Sbjct: 345 GSCTNSRIEDLRSAATILKNKKISKNIKAIVVPGSGSVKNQAEKEGLDKIFIDAGFEWRL 404
Query: 318 PGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITN 375
PGC CLG + D E ST+NRNF GR G +L SP+ AA +A+YG +
Sbjct: 405 PGCSMCLGMNNDKLSDGERCASTSNRNFEGRQGR-GGRTHLVSPIMAA-AAIYGHFVD 460
>dbj|BAA37138.1| (AB017135) isopropylmalate isomerase large subunit [Thermus
thermophilus]
Length = 472
Score = 139 bits (348), Expect = 4e-32
Identities = 121/465 (26%), Positives = 188/465 (40%), Gaps = 97/465 (20%)
Query: 4 VGKLLNAKPGEA-VIRK---------VDLVYAHDGTMPLIIEAFNRIFNEVRT--RAYIF 51
+GK L K EA +RK +DL H+ T P + VR R +
Sbjct: 1 MGKTLYEKVWEAHEVRKLKNGQSQLFIDLHLLHEVTSPQAFGMLKDLGLRVRYPHRTFAT 60
Query: 52 FDHVYPA-------PTVKVANLHREIREFAKIHGIPVVE----GKGISHQLVVELGLAEE 100
DH+ P ++ +R + HGI + +GI H + +LGL +
Sbjct: 61 VDHIVPTHDRTEPFQDPLAQSMLEALRANTREHGITFFDLGSGNQGIVHVIGPQLGLTQP 120
Query: 101 AKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMA 160
I GDSHT T GA G A G+G + V L + + + + ++G + A
Sbjct: 121 GMTIACGDSHTSTHGAFGAVAFGIGTSQVRDVLATQTLAAQKLKVRRINVEGRLAPGVYA 180
Query: 161 SDVMIHLITSLKDR-------EMSYNAIEFFNVPFSFDERLTLTNFSVEANAKTAII--- 210
DV++H+I L + E +A+E ++ + R+TL N S+E A+ +
Sbjct: 181 KDVILHIIRHLGVKGGLGYAYEYGGSAVEAMDM----ESRMTLCNMSIEGGARIGYVNPD 236
Query: 211 ---------------GEEYE-------------GGGYSDEITIELDSLPPMVAKPFSPAN 242
G E+E Y D +T + + P V +P
Sbjct: 237 ETTFQYLEGRPYVPKGSEWEEAKRRWLAWRSDPDASYDDVVTFRAEEIAPTVTWGITPGQ 296
Query: 243 GVSVED------------------------------VEGTKIDQVFIGSCTNGRFEQIKR 272
+ ++ ++G I FIGSCTN R ++
Sbjct: 297 AIPIDGRIPLLEELPEEERPVAEEALAYMGFRPGQPIKGVPIQVAFIGSCTNARLSDLRE 356
Query: 273 AAEILDGEQVN--VRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGV 330
A L G +V VR LV P S V R+ EEG+ + +AG PGC CL +
Sbjct: 357 VARYLKGHKVKKGVRALVVPGSEWVARKAEEEGIAEVFREAGFEWRMPGCSMCLAMNPDR 416
Query: 331 AGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITN 375
+E+ S++NRN++GRMGSP+ L SP+ A +A+ GEI +
Sbjct: 417 LEGDELCASSSNRNYKGRMGSPRGRTVLMSPLMVAAAAVAGEIAD 461
>sp|Q02142|LEU2_LACLA 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|486726|pir||S35134 probable 3-isopropylmalate
dehydratase (EC 4.2.1.33) chain leuC - Lactococcus
lactis subsp. lactis >gi|2565153|gb|AAB81915.1| (U92974)
LeuC [Lactococcus lactis]
Length = 460
Score = 136 bits (340), Expect = 3e-31
Identities = 115/438 (26%), Positives = 180/438 (40%), Gaps = 80/438 (18%)
Query: 13 GEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRTR--AYIFFDHVYPAPTV--------- 61
GE + +DL H+ T P + VR + Y DH P +
Sbjct: 21 GEPQLLYIDLHVIHEVTSPQAFQGLREAGRRVRRKDLTYGTLDHNVPTQNIFNIQDLISK 80
Query: 62 -KVANLHREIREF---AKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGAL 117
++ + ++EF A+ HG +G+GI H + E G + K IV GDSHT T GA
Sbjct: 81 KQIDTFTKNVKEFDVPAETHG---GKGQGIVHMVAPESGRTQPGKTIVCGDSHTATNGAF 137
Query: 118 GVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSL-KDREM 176
G A G+G ++V L IW P+ + + G P++ I + D ++ LI D +
Sbjct: 138 GAIAFGIGTSEVEHVLATQTIWQVKPKRMKIEFQGHPQKGIYSKDFILALIAKYGVDAGV 197
Query: 177 SYNAIEFFN---VPFSFDERLTLTNFSVEANAKTAII-----------GEEY-------- 214
Y A+E+ S +ER+T+ N S+E AK ++ G E+
Sbjct: 198 GY-AVEYSGDAISDLSMEERMTICNMSIEFGAKIGLMNPDEKTYDYVKGREHAPKNFDEA 256
Query: 215 ----------EGGGYSDEITIELDSLPPMVAKPFSPANGVSV------------------ 246
Y +++++ L PMV +P G+
Sbjct: 257 VSKWEKLVSDSDAQYDKILSLDVSQLKPMVTWGTNPGMGLEFGEKFPEINNDLNYERAYQ 316
Query: 247 -------EDVEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYR 297
+ + +FIGSCTN R ++ AA+I+ + + +V P S V
Sbjct: 317 YMDLKPGQTASDIDLGYIFIGSCTNARLGDLEEAAKIIGDRHIADGLTGIVVPGSRPVKE 376
Query: 298 RMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIY 357
+GL +I +AG PGC CLG + + ST+NRNF GR G A +
Sbjct: 377 AAEAQGLDKIFKEAGFEWREPGCSACLGMNPDQIPEYVHCASTSNRNFEGRQGH-NARTH 435
Query: 358 LASPVTAAVSALYGEITN 375
L SP AA +A+ G+ +
Sbjct: 436 LCSPAMAAAAAIAGKFVD 453
>gi|10957109 3-isopropylmalate dehydratase [Buchnera sp. APS]
>gi|7707823|dbj|BAA95425.1| (AP001071) 3-isopropylmalate
dehydratase [Buchnera sp. APS]
Length = 475
Score = 136 bits (339), Expect = 4e-31
Identities = 101/358 (28%), Positives = 162/358 (45%), Gaps = 68/358 (18%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + E G+ IV GDSHT T GA G + G+G ++V L + + +
Sbjct: 109 QGIVHVISPEKGMTLPGMTIVCGDSHTSTHGAFGALSFGIGTSEVEHVLATQTLKQQRFK 168
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFF-NV--PFSFDERLTLTNFSV 201
++ + + G ++++ A D+++ +I L + IEF NV S +ER+T+ N ++
Sbjct: 169 NMKIEITGEIQKFVTAKDLILFIIGKLGSSGGAGYIIEFCGNVIEKMSMEERMTICNMAI 228
Query: 202 EANAKTAIIGEEYEGGGY------------------------SDE-------ITIELDSL 230
E AK+ +I + Y SD+ + I + L
Sbjct: 229 EIGAKSGLIAPDEVTFSYLKNKMYAPRGVFWKKALNFWKNLKSDKNAFFDKVVNINISDL 288
Query: 231 PPMVAKPFSPANGVSVED-------------------------------VEGTKIDQVFI 259
P + +P +S++ + +D+VFI
Sbjct: 289 SPQITWGTNPDQVISIDQKIPDFSSFDNLIKKDLAKSACKYMGLKIGTYLTNITVDKVFI 348
Query: 260 GSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILP 317
GSCTNGR E ++ A++IL +++ NV+ +V P S +V R EGL +I I AG
Sbjct: 349 GSCTNGRIEDLRAASKILKDKKIANNVKAIVVPGSGSVKREAENEGLDKIFISAGFEWRL 408
Query: 318 PGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITN 375
PGC CLG + D E ST+NRNF GR G +L SP+ AA +A+YG+ +
Sbjct: 409 PGCSMCLGMNKDRLNDGERCASTSNRNFEGRQGR-GGRTHLVSPIMAAAAAVYGKFVD 465
>sp|P54384|LEU2_SYNY3 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7450354|pir||S76826 hypothetical protein -
Synechocystis sp. (strain PCC 6803)
>gi|1653827|dbj|BAA18738.1| (D90916) 3-isopropylmalate
dehydratase [Synechocystis sp.]
Length = 468
Score = 135 bits (336), Expect = 9e-31
Identities = 113/437 (25%), Positives = 179/437 (40%), Gaps = 83/437 (18%)
Query: 20 VDLVYAHDGTMP----LIIEAFNRIFNEVRTRAYIFFDHVYPAPTVK-------VANLHR 68
+ L H+ T P ++ E R+ RT A + DH+ P + + +
Sbjct: 28 IGLHLIHEVTSPQAFAMLKERGLRVMYPERTVATV--DHIVPTENQQRPFQDSLAEEMMQ 85
Query: 69 EIREFAKIHGI---PVVEG-KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGM 124
+ K H I P+ G +GI H + E GL + I GDSHT T GA G A G+
Sbjct: 86 ALEVNTKAHNIRFHPIGSGNQGIVHVIAPEQGLTQPGMTIACGDSHTSTHGAFGAIAFGI 145
Query: 125 GATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFF 184
G + V L + L + + ++G + A DV++H+I L + A E+
Sbjct: 146 GTSQVRDVLAAQSLSLNKLKVRKIEVNGDLAPGVYAKDVILHIIRKLGVKGGVGYAYEYA 205
Query: 185 NVPF---SFDERLTLTNFSVEANAKTAII-----------GEEYEGGG------------ 218
F + +ER+T+ N S+E A+ I G E+ G
Sbjct: 206 GSTFVAMNMEERMTVCNMSIEGGARCGYINPDEVTFAYLKGREFAPQGEDWDKAVQWWRS 265
Query: 219 --------YSDEITIELDSLPPMVAKPFSPANGVSVED---------------------- 248
Y D ++ + + P V +P G+ + +
Sbjct: 266 IASEPDAVYDDVVSFRAEDIEPTVTWGITPGQGIGISEPVPTLDSLNPDDQAIASEAYKY 325
Query: 249 --------VEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRR 298
++GTK+D FIGSCTNGR ++ AA++ G QV V+ V P S V ++
Sbjct: 326 MQFQPGQPLKGTKVDVCFIGSCTNGRLSDLEEAAKVAKGHQVAAGVKAFVVPGSEQVKQQ 385
Query: 299 MIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYL 358
GL +I AG GC CL + +++ S++NRNF+GR GS L
Sbjct: 386 AEAAGLDQIFQAAGFEWREAGCSMCLAMNPDKLQGDQLSASSSNRNFKGRQGSASGRTLL 445
Query: 359 ASPVTAAVSALYGEITN 375
SP A +A+ G++ +
Sbjct: 446 MSPAMVAAAAITGQVAD 462
>sp|P56934|LEU2_BUCAI 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|5912538|emb|CAB56192.1| (AJ006878) isopropylmalate
dehydratase subunit [Buchnera aphidicola]
Length = 471
Score = 134 bits (335), Expect = 1e-30
Identities = 100/358 (27%), Positives = 162/358 (44%), Gaps = 68/358 (18%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + E G+ IV GDSHT T GA G + G+ ++V L + + +
Sbjct: 105 QGIVHVISPEKGMTLPGVTIVCGDSHTSTHGAFGALSFGIDTSEVEHVLATQTLKQQRFK 164
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFF-NV--PFSFDERLTLTNFSV 201
++ + + G ++++ A D+++ +I L + IEF NV S +ER+T+ N ++
Sbjct: 165 NMKIEITGEIQKFVTAKDLILFIIGKLGSSGGAGYIIEFCGNVIEKMSMEERMTICNMAI 224
Query: 202 EANAKTAIIGEEYEGGGY------------------------SDE-------ITIELDSL 230
E AK+ +I + Y SD+ + I + L
Sbjct: 225 EIGAKSGLIAPDEVTFSYLKNKMYAPRGVFWKKALNFWKNLKSDKNAFFDKVVNINISDL 284
Query: 231 PPMVAKPFSPANGVSVED-------------------------------VEGTKIDQVFI 259
P + +P +S++ + +D+VFI
Sbjct: 285 SPQITWGTNPDQVISIDQKIPDFSSFDNLIKKDLAKSACKYMGLKIGTYLTNITVDEVFI 344
Query: 260 GSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILP 317
GSCTNGR E ++ A++IL +++ NV+ +V P S +V R EGL +I I+AG
Sbjct: 345 GSCTNGRIEDLRAASKILKDKKIANNVKAIVVPGSSSVKREAENEGLDKIFINAGFEWRL 404
Query: 318 PGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITN 375
PGC CLG + D E ST+NRNF GR G +L SP+ AA +A+YG+ +
Sbjct: 405 PGCSMCLGMNKDRLNDGERCASTSNRNFEGRQGR-GGRTHLVSPIMAAAAAVYGKFVD 461
>emb|CAC14578.1| (AJ296268) 2-isopropylmalate isomerase large subunit [Sinorhizobium
meliloti]
Length = 448
Score = 133 bits (332), Expect = 3e-30
Identities = 119/437 (27%), Positives = 181/437 (41%), Gaps = 82/437 (18%)
Query: 20 VDLVYAHDGTMPLIIEAFNRIFNEVRT--RAYIFFDHVYPAPT------------VKVAN 65
+D H+ T P E +VR + DH P ++V
Sbjct: 8 IDCHLVHEVTSPQAFEGLRMAGRKVRAPEKTLAVVDHNVPTSPDRHLGIKNEESRIQVEA 67
Query: 66 LHREIREFAKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMG 125
L R + + + +GI H + E G IV GDSHT T GA G A G+G
Sbjct: 68 LARNAADLGVEYYSENDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIG 127
Query: 126 ATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFN 185
++V L + K +++ V +DG + A D+++ +I + + + IEF
Sbjct: 128 TSEVEHVLATQTLIQKKAKNMLVRVDGQLPPGVTAKDIILAIIGEIGTAGGTGHVIEFAG 187
Query: 186 VPF---SFDERLTLTNFSVEANAKTAII------------------GEEY---------- 214
S + R+T+ N ++E A+ +I GE +
Sbjct: 188 EAIRSLSMEGRMTVCNMTIEGGARAGLIAPDETTFEYIKDKPRAPKGEAWDRAVEYWKTL 247
Query: 215 ---EGGGYSDEITIELDSLPPMVAKPFSPANGVSVEDV---------------------- 249
EG Y + ++ +LPP+V+ SP + VSV+ V
Sbjct: 248 HMDEGAHYDRVVVLDAANLPPIVSWGSSPEDVVSVQGVVPNPDDIQDETKRTSKWRALDY 307
Query: 250 ----EGTKI-----DQVFIGSCTNGRFEQIKRAAEILDGEQVN--VRTLVAPASINVYRR 298
GTKI D+VFIGSCTNGR E ++ AE+++G +V+ V ++ P S V +
Sbjct: 308 MGLKPGTKITDIAIDRVFIGSCTNGRIEDLRAVAEVVEGRKVSPTVSAMIVPGSGLVKEQ 367
Query: 299 MIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYL 358
EGL +I +AG PGC CL + E ST+NRNF GR G K +L
Sbjct: 368 AEAEGLDKIFKEAGFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGF-KGRTHL 426
Query: 359 ASPVTAAVSALYGEITN 375
SP AA +A+ G +
Sbjct: 427 LSPAMAAAAAVAGHFVD 443
>sp|P48573|LEU2_BUCRP 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|899390|emb|CAA50617.1| (X71612) isopropylmalate
isomerase subunit [Buchnera aphidicola]
Length = 471
Score = 132 bits (328), Expect = 8e-30
Identities = 102/356 (28%), Positives = 160/356 (44%), Gaps = 71/356 (19%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + E G+ IV GDSHT T GA G + G+G ++V L + + +
Sbjct: 105 QGIVHVIGPEQGMTLPGMTIVCGDSHTSTHGAFGALSFGIGTSEVEHVLVTQTLKQQRLK 164
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFN---VPFSFDERLTLTNFSV 201
++ + + G + ++ A D+++ +I L S IEF + +ER+T+ N ++
Sbjct: 165 NMKIQIKGKIKNFVTAKDIILFIIGKLGTSGGSGYVIEFCGDVIKKMNMEERMTVCNMAI 224
Query: 202 EANAKTAII-----------GEEYEGGG--------------------YSDEITIELDSL 230
E AK+A+I G Y G + E T ++ +L
Sbjct: 225 EMGAKSALIAPDETTFLYLKGRRYSPQGKFWEEAVKYWKTLITRSKAFFDKEFTFDVSNL 284
Query: 231 PPMVAKPFSPANGVSVED-------------------------------VEGTKIDQVFI 259
P + SP +S+ + + KID+VFI
Sbjct: 285 SPQITWGTSPDQVLSINEKIPDFNFFKDSVKKNLARSACDYMNLKPGSYLTDIKIDKVFI 344
Query: 260 GSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASIN--VYRRMIEEGLVRILIDAGVVI 315
GSCTN R E ++ AA+IL +++ N++ ++ P S V + +EGL +I IDAG
Sbjct: 345 GSCTNSRIEDLRSAAKILKNKKISKNIKAILLPGSGTGTVKKPAEKEGLDKIFIDAGFEW 404
Query: 316 LPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYG 371
PGC CLG + D E ST+NRNF GR G +L SP+ AA +A+YG
Sbjct: 405 RLPGCSMCLGMNNDKLSDGERCASTSNRNFEGRQGR-GGRTHLVSPIMAA-AAIYG 458
>pir||G81128 3-isopropylmalate dehydratase, large chain NMB1036 [imported] -
Neisseria meningitidis (group B strain MD58)
>gi|7226276|gb|AAF41435.1| (AE002454) 3-isopropylmalate
dehydratase, large subunit [Neisseria meningitidis MC58]
Length = 469
Score = 131 bits (327), Expect = 1e-29
Identities = 110/388 (28%), Positives = 168/388 (42%), Gaps = 70/388 (18%)
Query: 57 PAPTVKVANLHREIREFAKIHGIPVVE-GKGISHQLVVELGLAEEAKIIVGGDSHTPTLG 115
P ++V L + I+EF + P ++ G+GI H + E G +V GDSHT T G
Sbjct: 78 PISKLQVDTLDKNIKEFGALAYFPFMDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHG 137
Query: 116 ALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDRE 175
A G A G+G ++V + I K +S+ + +DG + + A DV +++I +
Sbjct: 138 AFGALAHGIGTSEVEHTMATQCITAKKSKSMLISVDGKLKAGVTAKDVALYIIGQIGTAG 197
Query: 176 MSYNAIEFFNVPF---SFDERLTLTNFSVEANAKTAII------------------GEEY 214
+ AIEF S + R+TL N ++EA A++ ++ GE +
Sbjct: 198 GTGYAIEFGGEAIRSLSMESRMTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAW 257
Query: 215 EGG--------------------------------GYSDEITIELDSLPPMVAKPFSPAN 242
+ G S E+ +++ S P A+ P
Sbjct: 258 DKAVEYWRTLVSDEGAVFDKEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVK 317
Query: 243 GVSVEDV-------EGTK-----IDQVFIGSCTNGRFEQIKRAAEILDGEQ--VNV-RTL 287
+E GT +D VFIGSCTN R E ++ AA I + NV R L
Sbjct: 318 RSGMERALEYMGLEAGTPLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVL 377
Query: 288 VAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRG 347
+ P S V + +EGL +I I+AG PGC CL + + ST+NRNF G
Sbjct: 378 IVPGSGLVKEQAEKEGLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEG 437
Query: 348 RMGSPKAEIYLASPVTAAVSALYGEITN 375
R G+ +L SP AA +A+ G T+
Sbjct: 438 RQGN-GGRTHLVSPAMAAAAAVTGRFTD 464
>pir||A81836 probable 3-isopropylmalate dehydratase (EC 4.2.1.33) large chain
NMA1450 [imported] - Neisseria meningitidis (group A
strain Z2491) >gi|7380103|emb|CAB84686.1| (AL162756)
putative 3-isopropylmalate dehydratase large subunit
[Neisseria meningitidis Z2491]
Length = 469
Score = 131 bits (325), Expect = 2e-29
Identities = 110/388 (28%), Positives = 168/388 (42%), Gaps = 70/388 (18%)
Query: 57 PAPTVKVANLHREIREFAKIHGIPVVE-GKGISHQLVVELGLAEEAKIIVGGDSHTPTLG 115
P ++V L + I+EF + P ++ G+GI H + E G +V GDSHT T G
Sbjct: 78 PISKLQVDTLDKNIKEFGALAYFPFMDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHG 137
Query: 116 ALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDRE 175
A G A G+G ++V + I K +S+ + +DG + + A DV +++I +
Sbjct: 138 AFGALAHGIGTSEVEHTMATQCITAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAG 197
Query: 176 MSYNAIEFFNVPF---SFDERLTLTNFSVEANAKTAII------------------GEEY 214
+ AIEF S + R+TL N ++EA A++ ++ GE +
Sbjct: 198 GTGYAIEFGGEAIRSLSMEGRMTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAW 257
Query: 215 EGG--------------------------------GYSDEITIELDSLPPMVAKPFSPAN 242
+ G S E+ +++ S P A+ P
Sbjct: 258 DKAVEYWRTLVSDEGAVFDKEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVK 317
Query: 243 GVSVEDV-------EGTK-----IDQVFIGSCTNGRFEQIKRAAEILDGEQ--VNV-RTL 287
+E GT +D VFIGSCTN R E ++ AA I + NV R L
Sbjct: 318 RSGMERALEYMGLEAGTPLNEIPVDIVFIGSCTNSRVEDLREAAAIAKDRKKAANVQRVL 377
Query: 288 VAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRG 347
+ P S V + +EGL +I I+AG PGC CL + + ST+NRNF G
Sbjct: 378 IVPGSGLVKEQAEKEGLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEG 437
Query: 348 RMGSPKAEIYLASPVTAAVSALYGEITN 375
R G+ +L SP AA +A+ G T+
Sbjct: 438 RQGN-GGRTHLVSPAMAAAAAVTGRFTD 464
>pir||D36889 3-isopropylmalate dehydratase (EC 4.2.1.33) chain leuC -
Lactococcus lactis subsp. lactis (strain IL1403)
Length = 459
Score = 131 bits (325), Expect = 2e-29
Identities = 114/438 (26%), Positives = 179/438 (40%), Gaps = 81/438 (18%)
Query: 13 GEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRTR--AYIFFDHVYPAPTV--------- 61
GE + +DL H+ T P + VR + Y DH P +
Sbjct: 21 GEPQLLYIDLHVIHEVTSPQAFQGLREAGRRVRRKDLTYGTLDHNVPTQDIFNIQDLISK 80
Query: 62 -KVANLHREIREF---AKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGAL 117
++ + ++EF A+ HG +G+GI H + E G + K IV GDSHT T GA
Sbjct: 81 KQIDTFTKNVKEFDVPAETHG---GKGQGIVHMVAPESGRTQPGKTIVCGDSHTATNGAF 137
Query: 118 GVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSL-KDREM 176
G A G+G ++V L IW P+ + + G P++ I + D ++ LI D +
Sbjct: 138 GAIAFGIGTSEVEHVLATQTIWQVKPKRMKIEFQGHPQKGIYSKDFILALIAKYGVDAGV 197
Query: 177 SYNAIEFFN---VPFSFDERLTLTNFSVEANAKTAII-----------GEEY-------- 214
Y A+E+ S +ER+T+ N S+E AK ++ G E+
Sbjct: 198 GY-AVEYSGDAISDLSMEERMTICNMSIEFGAKIGLMNPDEKTYDYVKGREHAPKNFDEA 256
Query: 215 ----------EGGGYSDEITIELDSLPPMVAKPFSPANGVSV------------------ 246
Y +++++ L PMV +P G+
Sbjct: 257 VSKWEKLVSDSDAQYDKILSLDVSQLKPMVTWGTNPGMGLEFGEKFPEINNDLNYERAYQ 316
Query: 247 -------EDVEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYR 297
+ + +FIGSCTN R ++ AA+I+ + + +V P S V
Sbjct: 317 YMDLKPGQTASAIDLGYIFIGSCTNARLGDLEEAAKIIGDRHIADGLTGIVVPGSRPVKE 376
Query: 298 RMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIY 357
+GL +I AG PGC CLG + + ST+NRNF GR G A +
Sbjct: 377 AAEAQGLDKI-FKAGFEWREPGCSACLGMNPDQIPEYVHCASTSNRNFEGRQGH-NARTH 434
Query: 358 LASPVTAAVSALYGEITN 375
+ SP AA +A+ G+ +
Sbjct: 435 VCSPAMAAAAAIAGKFVD 452
>gb|AAG31400.1| (AF197455) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 443
Score = 129 bits (320), Expect = 7e-29
Identities = 113/424 (26%), Positives = 187/424 (43%), Gaps = 89/424 (20%)
Query: 11 KPGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRT-----RAYIFFDH--------VYP 57
K EA++ +DL H+ T P +AFN + N+ R + + DH ++
Sbjct: 19 KNSEAILY-IDLHLLHEVTSP---QAFNALRNKKRKVRQSKKTFATMDHNVSTKIRSIHA 74
Query: 58 APTVKVANLHREIREFAKIHGIPVVE----GKGISHQLVVELGLAEEAKIIVGGDSHTPT 113
+ ++ + + I + + IP+ + +GI H + E G+ IV GDSHT T
Sbjct: 75 SGSMAQKQMEQLINNCTEFN-IPLYDINNPNQGIVHVIAPEKGMTLPGMTIVCGDSHTST 133
Query: 114 LGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKD 173
GA G A G+G ++V L + K +++ V + G +++ A D+++ +I L
Sbjct: 134 HGAFGTLAFGIGTSEVEHVLATQTLKQKRFKNMKVEIIGEMPKFVTAKDIILFIIGKLGS 193
Query: 174 REMSYNAIEFF-NV--PFSFDERLTLTNFSVEANAKTAIIGEEY---------------- 214
+ + IEF NV S +ER+T+ N +VE AK+ +I +
Sbjct: 194 SSGAGHVIEFCGNVIKNMSMEERMTICNMAVEMGAKSGLIAPDEITYKYLKDKIYSPFGI 253
Query: 215 --------------EGGGYSDE-ITIELDSLPPMVAKPFSPANGVSVED----------- 248
+ Y D+ +T+++ +L P + +P +S+++
Sbjct: 254 FWEKSLDYWKFLKSDKNAYFDKCVTVDISNLAPQITWGTNPDQVISIDEKIPDYNNINSI 313
Query: 249 --------------------VEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVRT 286
+ ID+VFIGSCTN R E ++ A++IL ++V +V
Sbjct: 314 VKRKSAKSACEYMGLQSNTYLTNISIDKVFIGSCTNARIEDLRSASKILKNKKVAKHVTA 373
Query: 287 LVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFR 346
+V P S V + EEGL +I IDAG PGC CLG + E S +NRNF
Sbjct: 374 IVVPGSGLVKNQAEEEGLDKIFIDAGFEWRLPGCSMCLGMNKDRLNFGERCASHSNRNFE 433
Query: 347 GRMG 350
GR G
Sbjct: 434 GRQG 437
>gb|AAG31391.1| (AF197452) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 443
Score = 128 bits (319), Expect = 9e-29
Identities = 95/333 (28%), Positives = 150/333 (44%), Gaps = 67/333 (20%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + E G+ IV GDSHT T GA G + G+G ++V L + K +
Sbjct: 105 QGIVHVIAPEKGMTLPGMTIVCGDSHTSTHGAFGALSFGIGTSEVEHVLATQTLKQKRFK 164
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNVPF---SFDERLTLTNFSV 201
++ + + G E+I A D+++ +I L + IEFF S +ER+TL N ++
Sbjct: 165 NMKITIIGKVPEFITAKDIILFIIGQLGSSRGTGYVIEFFGDVINNMSMEERMTLCNMAI 224
Query: 202 EANAKTAIIGEEY------------------------------EGGGYSDEI-TIELDSL 230
E AK+ +I + + Y D+ TI++ +L
Sbjct: 225 EMGAKSGLIAPDEITYSYLKNKIYSPYGIFWKKAIDYWSTLKSDKNAYFDKFFTIDISNL 284
Query: 231 PPMVAKPFSPANGVSVEDV--------------------------EGT-----KIDQVFI 259
P + +P +S+++ EGT +D+VFI
Sbjct: 285 APQITWGTNPDQVISIDEKIPNHNKFDSSIIRDSIKSACKYMGLKEGTYLTDVSVDKVFI 344
Query: 260 GSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILP 317
GSCTN R E ++ A++IL +++ NV+ +V P S +V + +EGL +I I+AG
Sbjct: 345 GSCTNARIEDLRSASKILKNKKISKNVKAIVVPGSGSVKEQAEKEGLDKIFINAGFEWRL 404
Query: 318 PGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMG 350
PGC CLG + E S +NRNF GR G
Sbjct: 405 PGCSMCLGMNNDRLNFGERCASXSNRNFEGRQG 437
>gb|AAG31397.1| (AF197454) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 444
Score = 128 bits (318), Expect = 1e-28
Identities = 111/424 (26%), Positives = 182/424 (42%), Gaps = 89/424 (20%)
Query: 11 KPGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRT--RAYIFFDHVYPAPTVKVA---- 64
K GE+++ +DL H+ T P +A +VR + + DH ++
Sbjct: 20 KNGESILY-IDLHLLHEVTSPQAFDALRSKKRKVRQSKKTFATMDHNVSTKIQSISASGS 78
Query: 65 -------NLHREIREFAKIHGIPVVE----GKGISHQLVVELGLAEEAKIIVGGDSHTPT 113
L + R+F IP+ + +GI H + E G+ IV GDSHT T
Sbjct: 79 MAKKQMEQLIKNCRDF----NIPLYDINNPNQGIVHVIAPEKGMTLPGMTIVCGDSHTST 134
Query: 114 LGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKD 173
GA G A G+G ++V L + K +++ V + G +++ A D+++ +I L
Sbjct: 135 HGAFGALAFGIGTSEVEHVLATQTLKQKRFKNMKVEIIGKIPKFVTAKDIILFIIGQLGS 194
Query: 174 REMSYNAIEFFN---VPFSFDERLTLTNFSVEANAKTAIIGEE-----------YEGGGY 219
+ IEF S +ER+T+ N ++E AK+ +I + Y G
Sbjct: 195 SSGTGYVIEFCGDVIKNISMEERMTICNMAIEMGAKSGLIAPDEITYKYLKDKIYSPSGL 254
Query: 220 -------------SDE-------ITIELDSLPPMVAKPFSPANGVSVED----------- 248
SD+ IT+++ +L P + +P +S+++
Sbjct: 255 FWEKSLDYWKFLKSDKNAHFDKCITLDISNLAPQITWGTNPDQVISIDEKIPDYNNINSL 314
Query: 249 --------------------VEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVRT 286
+ ID+VFIGSCTN R E ++ A++IL ++ +V+
Sbjct: 315 LKKESAKSACEYMGLKSNTYLTNISIDRVFIGSCTNARIEDLRAASKILKNRKIAKHVKA 374
Query: 287 LVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFR 346
+V P S +V R+ +EGL +I ID+G PGC CLG + E S +NRNF
Sbjct: 375 IVVPGSGSVKRKAEQEGLDKIFIDSGFEWRLPGCSMCLGMNKDRLNFGERCASHSNRNFE 434
Query: 347 GRMG 350
GR G
Sbjct: 435 GRQG 438
>pir||H82070 3-isopropylmalate dehydratase, large chain VC2492 [similarity] -
Vibrio cholerae (group O1 strain N16961)
>gi|9657072|gb|AAF95634.1| (AE004318) 3-isopropylmalate
dehydratase, large subunit [Vibrio cholerae]
Length = 467
Score = 128 bits (318), Expect = 1e-28
Identities = 113/446 (25%), Positives = 179/446 (39%), Gaps = 81/446 (18%)
Query: 10 AKPGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVR--TRAYIFFDHVYPAPT------- 60
A PGE I +D H+ T P + VR ++ + DH T
Sbjct: 19 AAPGETPILYIDRHLVHEVTSPQAFDGLREKGRPVRQVSKTFATMDHNVSTTTKDINASG 78
Query: 61 ----VKVANLHREIREFAKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGA 116
+++ L + EF + +GI H + ELG+ IV GDSHT T GA
Sbjct: 79 EMARIQMQTLSKNCEEFGVTLYDINHKYQGIVHVMGPELGITLPGMTIVCGDSHTATHGA 138
Query: 117 LGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREM 176
G A G+G ++V L + +++ + + G I A D+++ +I
Sbjct: 139 FGSLAFGIGTSEVEHVLATQTLKQGRAKTMKIEVRGKVAPGITAKDIVLAIIGKTTAAGG 198
Query: 177 SYNAIEFFNVPF---SFDERLTLTNFSVEANAKTAIIGEEYEGGGY-------------- 219
+ +EF S + R+T+ N ++E AK +I + Y
Sbjct: 199 TGYVVEFCGEAIRDLSMEGRMTVCNMAIELGAKAGLIAPDATTFNYIKGRKFAPQGSDWD 258
Query: 220 ----------SDE-------ITIELDSLPPMVAKPFSPANGVSVED-------------- 248
+DE +T+E + P V +P ++V++
Sbjct: 259 AAVDYWQTLKTDEDAQFDAVVTLEASEIKPQVTWGTNPGQVIAVDEPIPSPSQFADPVER 318
Query: 249 -----------------VEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVRTLVA 289
+ K+D+VF+GSCTN R E ++ AA + G++V +V+ L+
Sbjct: 319 SSAEKALAYMGLEAGKMLSDYKVDKVFVGSCTNSRIEDMRAAAAVAKGKKVASHVQALIV 378
Query: 290 PASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRM 349
P S V + EGL +I I+AG PGC CL + G E ST+NRNF GR
Sbjct: 379 PGSEQVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLGPGERCASTSNRNFEGRQ 438
Query: 350 GSPKAEIYLASPVTAAVSALYGEITN 375
G +L SP AA +A+ G +
Sbjct: 439 GR-DGRTHLVSPAMAAAAAIAGHFVD 463
>sp|P49601|LEU2_USTMA 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMALATE ISOMERASE)
(ALPHA-IPM ISOMERASE) (IPMI) >gi|311008|gb|AAA34226.1|
(L20832) LEU1 [Ustilago maydis]
Length = 773
Score = 127 bits (317), Expect = 2e-28
Identities = 101/359 (28%), Positives = 155/359 (43%), Gaps = 69/359 (19%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + E G IV GDSHT T GA G A G+G ++V L + K +
Sbjct: 112 QGIVHIIGPEQGFTLPGATIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLLQKRAK 171
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNVPF---SFDERLTLTNFSV 201
++ + +DG + + + D+++H+I + + + IE+ S + R+++ N S+
Sbjct: 172 NMLIQVDGELSQGVTSKDIILHIIGLIGTAGGTGHVIEYAGSTIRSLSMEARMSICNMSI 231
Query: 202 EANAKTAII-------------------GEEY-------------EGGGYSDEITIELDS 229
EA A+ +I GE + EG Y I I+
Sbjct: 232 EAGARAGLIAPDEITYEYIKGRPLAPKQGEAWDQALAYWKTLPSDEGAQYDTVIKIDAKD 291
Query: 230 LPPMVAKPFSPANGVSVED-------------------------------VEGTKIDQVF 258
+PP V SP + V++ +E KID+VF
Sbjct: 292 IPPTVTWGTSPQDVVAITGTVPDPKNASNEAEAKAWTRALEYMGLEAGTPMEKIKIDKVF 351
Query: 259 IGSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVIL 316
IGSCTN R E ++ AA +L G +V + ++ P S V ++ EGL +I AG
Sbjct: 352 IGSCTNARIEDLRAAARVLHGRKVADGLYCMLVPGSGLVKKQAEAEGLDKIFQAAGFDWR 411
Query: 317 PPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITN 375
GC CLG + E ST+NRNF GR G+ +L SP AA A+ G +T+
Sbjct: 412 EAGCSMCLGMNPDQLAPGERCASTSNRNFEGRQGA-GGRTHLMSPAMAAACAVTGYLTD 469
>sp|P55811|LEU2_RHINI 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMALATE ISOMERASE)
(ALPHA-IPM ISOMERASE) (IPMI) >gi|1817566|dbj|BAA09893.1|
(D63833) 3-isopropylmalate dehyratase [Rhizopus niveus]
>gi|1588638|prf||2209244A alpha-isopropylmalate
isomerase [Rhizopus niveus]
Length = 750
Score = 127 bits (316), Expect = 2e-28
Identities = 102/358 (28%), Positives = 156/358 (43%), Gaps = 68/358 (18%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + E G +V GDSHT T GA G A G+G ++V L + K +
Sbjct: 111 QGIVHVIGPEQGFTLPGTTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLLQKKSK 170
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFF---NVPFSFDERLTLTNFSV 201
++ + ++G P + + D+ +H+I + + IEF N S + R+++ N S+
Sbjct: 171 NMRIRVEGKPSAGVTSKDIALHVIGVIGTAGGTGCVIEFCGEANESLSMESRMSICNMSI 230
Query: 202 EANAKTAII------------------GEEYE-------------GGGYSDEITIELDSL 230
EA A+ +I GEE++ Y + I+ +
Sbjct: 231 EAGARAGMIAPDDITFEYLCNKPLASKGEEWDRAVAYWKTLKFDADAQYDITVDIKASDI 290
Query: 231 PPMVAKPFSPANGV----------SVED---------------------VEGTKIDQVFI 259
P V SP + S+ D +EG KID+VFI
Sbjct: 291 APTVTWGTSPQDVAPITGKTPDLDSIADPLRRLAVQRALDYIGIASNTPLEGVKIDKVFI 350
Query: 260 GSCTNGRFEQIKRAAEILDGEQVN--VRTLVAPASINVYRRMIEEGLVRILIDAGVVILP 317
GSCTN R E + AA I+ G++V V +V P S V R+ +GL +I +AG
Sbjct: 351 GSCTNSRIEDLGPAAAIVKGKRVADWVDAMVVPGSALVKRQAERKGLDKIFQEAGFNWRE 410
Query: 318 PGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITN 375
GC CLG + E ST+NRNF+GR G+ +L SP AA + + G +T+
Sbjct: 411 AGCSMCLGMNPDQLKPGERCASTSNRNFKGRQGA-GGRTHLLSPAMAAAAGIKGCLTD 467
>sp|P44968|LEU2_HAEIN 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|1074594|pir||F64163 3-isopropylmalate dehydratase
(EC 4.2.1.33) chain leuC - Haemophilus influenzae
(strain Rd KW20) >gi|1574017|gb|AAC22649.1| (U32779)
3-isopropylmalate dehydratase, alpha subunit (leuC)
[Haemophilus influenzae Rd]
Length = 469
Score = 126 bits (313), Expect = 5e-28
Identities = 117/444 (26%), Positives = 181/444 (40%), Gaps = 82/444 (18%)
Query: 13 GEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVR--TRAYIFFDHVYPAPTVKVANLHR-- 68
GE I ++ H+ T P + +VR + + DH V L
Sbjct: 20 GETPILYINRHLIHEVTSPQAFDGLRVANRQVRQVNKTFGTMDHSISTQVRDVNKLEGQA 79
Query: 69 -----EIREFAKIHGIPVVE----GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGV 119
E+ + K GI + + +GI H + E GL IV GDSHT T GA G
Sbjct: 80 KIQVLELDKNTKATGIKLFDITTKEQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGA 139
Query: 120 FAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYN 179
A G+G ++V L + +S+ + + G I A D+++ +I + +
Sbjct: 140 LAFGIGTSEVEHVLATQTLKQARAKSMKIEVRGKVASGITAKDIILAIIGKTTMAGGTGH 199
Query: 180 AIEFFNVPF---SFDERLTLTNFSVEANAKTAII------------------GEEYEGG- 217
+EF S + R+T+ N ++E AK +I G+++E
Sbjct: 200 VVEFCGEAIQDLSMEGRMTVCNMAIEMGAKAGLIAPDETTFAYLKDRPHAPKGKDWEDAV 259
Query: 218 ------------GYSDEITIELDSLPPMVAKPFSPANGVSVED----------------- 248
+ +T+E + P V +P +SV +
Sbjct: 260 AYWKTLKSDDDAQFDTVVTLEAKDIAPQVTWGTNPGQVISVNETIPNPQEMADPVQRASA 319
Query: 249 --------------VEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQV--NV-RTLVAPA 291
++ K+DQVFIGSCTN R E ++ AA ++ G + NV R LV P
Sbjct: 320 EKALHYIGLEAGTNLKDIKVDQVFIGSCTNSRIEDLRAAAAVMKGRKKADNVKRILVVPG 379
Query: 292 SINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGS 351
S V + +EGL +I I AG PGC CLG + G+ E ST+NRNF GR G
Sbjct: 380 SGLVKEQAEKEGLDKIFIAAGAEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGR 439
Query: 352 PKAEIYLASPVTAAVSALYGEITN 375
+L SP AA + ++G+ +
Sbjct: 440 -NGRTHLVSPAMAAAAGVFGKFVD 462
>gb|AAG31382.1| (AF197449) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 442
Score = 125 bits (312), Expect = 6e-28
Identities = 107/415 (25%), Positives = 174/415 (41%), Gaps = 88/415 (21%)
Query: 20 VDLVYAHDGTMPLIIEAFNRIFNEVRT--RAYIFFDHVYPAPTVKVA-----------NL 66
+DL H+ T P +A +VR + + DH ++ L
Sbjct: 27 IDLHLLHEVTSPQAFDALRNKQRKVRQSRKTFATMDHNVSTTIQNISASGSMAQKQMEQL 86
Query: 67 HREIREFAKIHGIPVVE----GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAV 122
+ +EF IP+ + +GI H + E G+ IV GDSHT T GA G A
Sbjct: 87 IKNCQEF----NIPLYDINNPNQGIVHVIAPEKGMTLPGMTIVCGDSHTSTHGAFGALAF 142
Query: 123 GMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIE 182
G+G ++V L + K +++ + + G +++ A D+++ +I L + IE
Sbjct: 143 GIGTSEVEHVLATQTLKQKRFKNMKIEIIGEIPKFVTAKDIILFIIGKLGSSSGTGYVIE 202
Query: 183 FFN---VPFSFDERLTLTNFSVEANAKTAIIGEE-----------YEGGGYSDE------ 222
F S +ER+T+ N ++E AK+ +I + Y G S E
Sbjct: 203 FCGDVIKNMSMEERMTICNMAIEMGAKSGLIAPDEITYKYLKNKIYSPSGLSWEKSLDHW 262
Query: 223 --------------ITIELDSLPPMVAKPFSPANGVSVED-------------------- 248
+T+++ +L P + +P +S+++
Sbjct: 263 KFLKSDKNAYFDKCVTVDISNLAPQITWGTNPDQVISIDEKIPDYNNINSAIKRESSKSA 322
Query: 249 -----------VEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINV 295
+ ID+VFIGSCTN R E ++ A++IL +++ +V+ +V P S V
Sbjct: 323 CEYMGLQSNTYLTNISIDRVFIGSCTNARIEDLRSASKILKNKKIAKHVKAIVVPGSKLV 382
Query: 296 YRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMG 350
R+ +EGL RI IDAG PGC CLG + S +NRNF GR G
Sbjct: 383 KRQAEQEGLDRIFIDAGFEWRLPGCSMCLGMNKDRLHFGGRCASXSNRNFEGRQG 437
>sp|O14289|LEU2_SCHPO 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMALATE ISOMERASE)
(ALPHA-IPM ISOMERASE) (IPMI) >gi|7489951|pir||T39210
3-isopropylmalate dehydratase - fission yeast
(Schizosaccharomyces pombe) >gi|2414659|emb|CAB16402.1|
(Z99262) 3-isopropylmalate dehydratase
[Schizosaccharomyces pombe]
Length = 758
Score = 125 bits (311), Expect = 8e-28
Identities = 105/387 (27%), Positives = 164/387 (42%), Gaps = 69/387 (17%)
Query: 57 PAPTVKVANLHREIREFAKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGA 116
P +V L I+EF + +GI H + E G +V GDSHT T GA
Sbjct: 89 PDSRTQVLALENNIKEFGLTYYGMNDRRQGIVHVIGPEQGFTLPGTTLVCGDSHTSTHGA 148
Query: 117 LGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREM 176
G A G+G ++V L I + +++ + ++G E I + D+++H+I +
Sbjct: 149 FGALAFGIGTSEVEHVLATQTILQRKSKNMRIRVNGKLPEGIASKDLILHIIGVIGTAGG 208
Query: 177 SYNAIEFFNVPF---SFDERLTLTNFSVEANAKTAII------------------GEEYE 215
+ + IEF S + R+++ N S+EA A+ +I G+++E
Sbjct: 209 TGSVIEFCGEAIEGLSMEARMSMCNMSIEAGARAGMIAPDATTFEYVKNRPLAPKGDDWE 268
Query: 216 -------------GGGYSDEITIELDSLPPMVAKPFSPANGVSVE--------------- 247
Y E+ I + P V SP + + +
Sbjct: 269 QAVAYWKTLRSDENAKYDIEVEINAADVLPTVTWGTSPQDVIPINGNIPDPAHVKDNVRA 328
Query: 248 ----------------DVEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVR-TLV 288
+ ID+VFIGSCTN R E ++ AA ++ G +V NV+ ++
Sbjct: 329 ASIQRSLEYMGLKPNTSIVSYPIDKVFIGSCTNSRIEDLRLAAAVVKGRKVAANVKDAMI 388
Query: 289 APASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGR 348
P S V + EGL +I I+AG GC CLG + E ST+NRNF GR
Sbjct: 389 VPGSGLVKKMAEAEGLDQIFIEAGFDWREAGCSMCLGMNPDQLKPYERCASTSNRNFEGR 448
Query: 349 MGSPKAEIYLASPVTAAVSALYGEITN 375
G+ K +L SP AA +A+ G + N
Sbjct: 449 QGA-KGRTHLVSPAMAAAAAIKGHLCN 474
>pir||S40586 3-isopropylmalate dehydratase (EC 4.2.1.33) alpha chain -
Escherichia coli >gi|216490|dbj|BAA01341.1| (D10483)
isopropylmalate isomerase chain leuC homolog(PIR:S10171)
[Escherichia coli]
Length = 466
Score = 125 bits (311), Expect = 8e-28
Identities = 99/358 (27%), Positives = 151/358 (41%), Gaps = 68/358 (18%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + E G+ IV GDSHT T GA G A G+G ++V L + +
Sbjct: 105 QGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAK 164
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNVPF---SFDERLTLTNFSV 201
++ + + G I A D+++ +I + + +EF S + R+TL N ++
Sbjct: 165 TMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAI 224
Query: 202 EANAKTAII------------------GEEY-------------EGGGYSDEITIELDSL 230
E AK ++ G+++ EG + +T++ + +
Sbjct: 225 EMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEI 284
Query: 231 PPMVAKPFSPANGVSVED-------------------------------VEGTKIDQVFI 259
P V +P +SV D + ID+VFI
Sbjct: 285 SPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPLTEVAIDKVFI 344
Query: 260 GSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILP 317
GSCTN R E ++ AAEI+ G +V V+ LV P S V + EGL +I I+AG
Sbjct: 345 GSCTNSRIEDLRAAAEIVKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRL 404
Query: 318 PGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITN 375
PGC CL + E ST+NRNF GR G +L SP AA +A+ G +
Sbjct: 405 PGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGR-GGRTHLVSPAMAAAAAVTGHFAD 461
>sp|P30127|LEU2_ECOLI 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
Length = 466
Score = 125 bits (311), Expect = 8e-28
Identities = 99/358 (27%), Positives = 151/358 (41%), Gaps = 68/358 (18%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + E G+ IV GDSHT T GA G A G+G ++V L + +
Sbjct: 105 QGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAK 164
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNVPF---SFDERLTLTNFSV 201
++ + + G I A D+++ +I + + +EF S + R+TL N ++
Sbjct: 165 TMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAI 224
Query: 202 EANAKTAII------------------GEEY-------------EGGGYSDEITIELDSL 230
E AK ++ G+++ EG + +T++ + +
Sbjct: 225 EMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEI 284
Query: 231 PPMVAKPFSPANGVSVED-------------------------------VEGTKIDQVFI 259
P V +P +SV D + ID+VFI
Sbjct: 285 SPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPLTEVAIDKVFI 344
Query: 260 GSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILP 317
GSCTN R E ++ AAEI+ G +V V+ LV P S V + EGL +I I+AG
Sbjct: 345 GSCTNSRIEDLRAAAEIVKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRL 404
Query: 318 PGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITN 375
PGC CL + E ST+NRNF GR G +L SP AA +A+ G +
Sbjct: 405 PGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGR-GGRTHLVSPAMAAAAAVTGHFAD 461
>pir||H64728 3-isopropylmalate dehydratase (EC 4.2.1.33) alpha chain -
Escherichia coli >gi|1786259|gb|AAC73183.1| (AE000117)
3-isopropylmalate isomerase (dehydratase) subunit
[Escherichia coli K12]
Length = 466
Score = 124 bits (309), Expect = 1e-27
Identities = 99/358 (27%), Positives = 150/358 (41%), Gaps = 68/358 (18%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + E G+ IV GDSHT T GA G A G+G ++V L + +
Sbjct: 105 QGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAK 164
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNVPF---SFDERLTLTNFSV 201
++ + + G I A D+++ +I + + +EF S + R+TL N ++
Sbjct: 165 TMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAI 224
Query: 202 EANAKTAII------------------GEEY-------------EGGGYSDEITIELDSL 230
E AK ++ G+++ EG + +T++ + +
Sbjct: 225 EMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEI 284
Query: 231 PPMVAKPFSPANGVSVED-------------------------------VEGTKIDQVFI 259
P V +P +SV D + ID+VFI
Sbjct: 285 SPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPLTEVAIDKVFI 344
Query: 260 GSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILP 317
GSCTN R E ++ AAEI G +V V+ LV P S V + EGL +I I+AG
Sbjct: 345 GSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRL 404
Query: 318 PGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITN 375
PGC CL + E ST+NRNF GR G +L SP AA +A+ G +
Sbjct: 405 PGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGR-GGRTHLVSPAMAAAAAVTGHFAD 461
>gb|AAG31388.1| (AF197451) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 442
Score = 123 bits (307), Expect = 2e-27
Identities = 96/333 (28%), Positives = 148/333 (43%), Gaps = 67/333 (20%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + E G+ IIV GDSHT T GA G + G+G ++V L + + +
Sbjct: 105 QGIVHVIAPEKGMTLPGMIIVCGDSHTSTHGAFGALSFGIGTSEVEHVLATQTLKQQCFK 164
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFF-NV--PFSFDERLTLTNFSV 201
++ V + G +++ A D+++ +I L S IEF NV S +ER+T+ N ++
Sbjct: 165 NMKVEIVGDIPKFVTAKDIILFIIRRLGSSGGSGYVIEFCGNVIEKMSMEERMTVCNMAI 224
Query: 202 EANAKTAIIGEEYEGGGY------------------------SDE-------ITIELDSL 230
E AK+ II + Y SD+ TI++ +L
Sbjct: 225 EMGAKSGIIAPDETTYAYLKNKIYSPSGACWEXSISYWKTLKSDKDAFFDKCFTIDISNL 284
Query: 231 PPMVAKPFSPANGVSVED-------------------------------VEGTKIDQVFI 259
P V SP +S+ + + ID+VFI
Sbjct: 285 APQVTWGTSPDQVISINEKIPHYNEFNSITQKNSAKSALKYMGLNEGAYLTNISIDKVFI 344
Query: 260 GSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILP 317
GSCTN R E ++ A++IL ++ NV+ +V P S +V ++ EGL +I I++G
Sbjct: 345 GSCTNARIEDLRSASKILKNRKISSNVKAIVVPGSGSVKQQAEREGLDKIFINSGFEWRL 404
Query: 318 PGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMG 350
PGC CLG + E S +NRNF GR G
Sbjct: 405 PGCSMCLGMNRDQLNIGERCASXSNRNFEGRQG 437
>emb|CAB83173.1| (AL162692) aconitate hydratase [Schizosaccharomyces pombe]
Length = 905
Score = 123 bits (306), Expect = 3e-27
Identities = 107/368 (29%), Positives = 160/368 (43%), Gaps = 59/368 (16%)
Query: 63 VANLHREIREF----AKIHGIPVV-EGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGAL 117
+AN ++EI +F AK +GI G GI HQ+V+E A +++G DSHTP G L
Sbjct: 144 IAN-NKEIFDFLQSAAKKYGIQFWGPGSGIIHQIVLE-NYAAPGGMMLGTDSHTPNAGGL 201
Query: 118 GVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMS 177
G+ A+G+G D A+ LK P+ I V L G+ + D+++HL L R +
Sbjct: 202 GMIAIGVGGADAVDAMTNTPWELKAPKIIGVNLTGAMSGWTTPKDLILHLAGKLTVRGGT 261
Query: 178 YNAIEFFN---VPFSFDERLTLTNFSVEANAKTAI------------------------- 209
+ IE+F S T+ N E A T+I
Sbjct: 262 GHIIEYFGPGVASLSCTGMATVCNMGAEVGATTSIFPYTDSMRRYLIATHRAEVADAASE 321
Query: 210 IGEEYE------GGGYSDEITIELDSLPPMVAKPFSPANGVSV----EDVEGTK----ID 255
+ EY G Y I I L L P + PF+P V E +E K +
Sbjct: 322 VHSEYNYLAADTGAKYDQIIDINLSELTPSLNGPFTPDLSTPVSKFGEAIEKNKWPKKLS 381
Query: 256 QVFIGSCTNGRFEQIKRAAEILD-----GEQVNVRTLVAPASINVYRRMIEEGLVRILID 310
IGSCTN ++ + ++++ G + V LV P S + + +G+ L +
Sbjct: 382 AGLIGSCTNSSYQDMTCVVDVVEQAISAGLKPKVPFLVTPGSEQIRATIERDGITERLEE 441
Query: 311 AGVVILPPGCGPCLGRHMG----VAGDNEVILSTTNRNFRGRM-GSPKAEIYLASPVTAA 365
AG +L CGPC+G +G+ IL++ NRNFR R G+P +L SPV A
Sbjct: 442 AGATVLANACGPCIGMWKRTDDIASGEPNAILTSFNRNFRSRNDGNPSTMNFLTSPVIVA 501
Query: 366 VSALYGEI 373
++
Sbjct: 502 AKIFSSDL 509
>gb|AAG31406.1| (AF197457) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 448
Score = 123 bits (306), Expect = 3e-27
Identities = 95/345 (27%), Positives = 153/345 (43%), Gaps = 68/345 (19%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + E G+ IV GDSHT T GA G + G+G ++V L + + +
Sbjct: 105 QGIVHVISPEKGMTLPGMTIVCGDSHTSTHGAFGALSFGIGTSEVEHVLATQTLKQQRFK 164
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFF-NV--PFSFDERLTLTNFSV 201
++ + + G ++++ A D+++ +I L + IEF NV S +ER+T+ N ++
Sbjct: 165 NMKIEITGEIQKFVTAKDLILFIIGKLGSSGGAGYIIEFCGNVIEKMSMEERMTICNMAI 224
Query: 202 EANAKTAIIGEEYEGGGY------------------------SDE-------ITIELDSL 230
E AK+ +I + Y SD+ + I + L
Sbjct: 225 EIGAKSGLIAPDEVTFSYLKNKMYAPRGVFWKKALNFWKNLKSDKNAFFDKVVNINISDL 284
Query: 231 PPMVAKPFSPANGVSVED-------------------------------VEGTKIDQVFI 259
P + +P +S++ + +D+VFI
Sbjct: 285 SPQITWGTNPDQVISIDQKIPDFSSFDNLIKKDLAKSACKYMGLKIGTYLTNITVDKVFI 344
Query: 260 GSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILP 317
GSCTNGR E ++ A++IL +++ NV+ +V P S +V R EGL +I I+AG
Sbjct: 345 GSCTNGRIEDLRAASKILKDKKIANNVKAIVVPGSGSVKREAENEGLDKIFINAGFEWRL 404
Query: 318 PGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPV 362
PGC CLG + D E S +NRNF GR G +L SP+
Sbjct: 405 PGCSMCLGMNKDRLNDGERCASHSNRNFEGRQGR-XXRTHLVSPI 448
>pir||S26864 3-isopropylmalate dehydratase (EC 4.2.1.33) - Phycomyces
blakesleeanus >gi|3113|emb|CAA37257.1| (X53090)
alpha-isopropylmalate isomerase (AA 1-689) [Phycomyces
blakesleeanus]
Length = 689
Score = 123 bits (305), Expect = 4e-27
Identities = 116/444 (26%), Positives = 173/444 (38%), Gaps = 91/444 (20%)
Query: 20 VDLVYAHDGTMPLIIEAFNRIFNEVR----TRAYIFFDHVYPAPTVK------------- 62
+D H+ T P E VR T A + DH P T K
Sbjct: 15 IDRHLVHEVTSPQAFEGLRNASRRVRRPDCTLATV--DHNIPTTTRKKFKSITTFIDEAD 72
Query: 63 ----VANLHREIREFAKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALG 118
L ++EF + +GI H + E G +V GDSHT T GA G
Sbjct: 73 SRTQCETLETNVKEFELTYFGMEDSRQGIVHVIGPEQGFTLPGTTVVCGDSHTSTHGAFG 132
Query: 119 VFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSY 178
A G+G ++V L + K +++ V ++G + + DV +H+I + +
Sbjct: 133 ALAFGIGTSEVEHVLATQTLLQKKSKNMRVCVEGELTPGVTSKDVALHVIGLIGTAGGTR 192
Query: 179 NAIEFFN---VPFSFDERLTLTNFSVEANAKTAII------------------GEEYE-- 215
IEF S + R+++ N S+E A+ +I G E++
Sbjct: 193 CVIEFCGSAIASLSMEARMSICNMSIEGGARAGMIAPDEITFEYLRGRPLAPEGAEWDKA 252
Query: 216 -----------GGGYSDEITIELDSLPPMVAKPFSPANGV----------SVED------ 248
Y ++ I + P + SP + SV D
Sbjct: 253 VQYWKSLKSDPNAKYDIDVKIAASDIAPTITWGTSPQDVAPITANVPDPSSVSDPARKAA 312
Query: 249 ---------------VEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQVN--VRTLVAPA 291
+E KID+ FIGSCTN R E ++ AA I+ G+ + V +V P
Sbjct: 313 MERALEYIGLVPNTPLEEVKIDKAFIGSCTNSRIEDLRSAASIVKGKHIADWVYAMVVPG 372
Query: 292 SINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGS 351
S V R+ EGL ++ DAG GC CLG + E ST+NRNF GR G+
Sbjct: 373 SGLVKRQAEREGLDKVFTDAGFDWREAGCSMCLGMNPDQLSPGERCASTSNRNFEGRQGA 432
Query: 352 PKAEIYLASPVTAAVSALYGEITN 375
+L SP AA +A+ G +T+
Sbjct: 433 -GGRTHLMSPAMAAAAAIRGYLTD 455
>sp|P18250|LEU2_PHYBL 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMALATE ISOMERASE)
(ALPHA-IPM ISOMERASE) (IPMI)
Length = 744
Score = 123 bits (305), Expect = 4e-27
Identities = 116/444 (26%), Positives = 173/444 (38%), Gaps = 91/444 (20%)
Query: 20 VDLVYAHDGTMPLIIEAFNRIFNEVR----TRAYIFFDHVYPAPTVK------------- 62
+D H+ T P E VR T A + DH P T K
Sbjct: 15 IDRHLVHEVTSPQAFEGLRNASRRVRRPDCTLATV--DHNIPTTTRKKFKSITTFIDEAD 72
Query: 63 ----VANLHREIREFAKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALG 118
L ++EF + +GI H + E G +V GDSHT T GA G
Sbjct: 73 SRTQCETLETNVKEFELTYFGMEDSRQGIVHVIGPEQGFTLPGTTVVCGDSHTSTHGAFG 132
Query: 119 VFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSY 178
A G+G ++V L + K +++ V ++G + + DV +H+I + +
Sbjct: 133 ALAFGIGTSEVEHVLATQTLLQKKSKNMRVCVEGELTPGVTSKDVALHVIGLIGTAGGTR 192
Query: 179 NAIEFFN---VPFSFDERLTLTNFSVEANAKTAII------------------GEEYE-- 215
IEF S + R+++ N S+E A+ +I G E++
Sbjct: 193 CVIEFCGSAIASLSMEARMSICNMSIEGGARAGMIAPDEITFEYLRGRPLAPEGAEWDKA 252
Query: 216 -----------GGGYSDEITIELDSLPPMVAKPFSPANGV----------SVED------ 248
Y ++ I + P + SP + SV D
Sbjct: 253 VQYWKSLKSDPNAKYDIDVKIAASDIAPTITWGTSPQDVAPITANVPDPSSVSDPARKAA 312
Query: 249 ---------------VEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQVN--VRTLVAPA 291
+E KID+ FIGSCTN R E ++ AA I+ G+ + V +V P
Sbjct: 313 MERALEYIGLVPNTPLEEVKIDKAFIGSCTNSRIEDLRSAASIVKGKHIADWVYAMVVPG 372
Query: 292 SINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGS 351
S V R+ EGL ++ DAG GC CLG + E ST+NRNF GR G+
Sbjct: 373 SGLVKRQAEREGLDKVFTDAGFDWREAGCSMCLGMNPDQLSPGERCASTSNRNFEGRQGA 432
Query: 352 PKAEIYLASPVTAAVSALYGEITN 375
+L SP AA +A+ G +T+
Sbjct: 433 -GGRTHLMSPAMAAAAAIRGYLTD 455
>sp|P17279|LEU2_RHIRA 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMALATE ISOMERASE)
(ALPHA-IPM ISOMERASE) (IPMI) >gi|82770|pir||JQ0160
3-isopropylmalate dehydratase (EC 4.2.1.33) - Rhizomucor
circinelloides >gi|168367|gb|AAA33422.1| (M33166)
alpha-isopropylmalate isomerase (put.); putative [Mucor
circinelloides]
Length = 644
Score = 123 bits (305), Expect = 4e-27
Identities = 114/444 (25%), Positives = 176/444 (38%), Gaps = 91/444 (20%)
Query: 20 VDLVYAHDGTMPLIIEAFNRIFNEVR----TRAYIFFDHVYPAPTVKV------------ 63
+D H+ T P E VR T A + DH P T K+
Sbjct: 74 IDRHLVHEVTSPQAFEGLRNANRPVRRPDCTLATV--DHNIPTTTRKIFKNITTFIKEAD 131
Query: 64 -----ANLHREIREFAKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALG 118
L + I F + +GI H + E G A +V GDSHT T GA G
Sbjct: 132 SRTQCETLEQNIEAFGLTYFGMEDSRQGIVHVIGPEQGFTLPATTVVCGDSHTSTHGAFG 191
Query: 119 VFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSY 178
A G+G ++V L + K +++ + + G + + D+++H+I + +
Sbjct: 192 ALAFGIGTSEVEHVLATQTLLQKKSKNMRIRVQGKALPGVTSKDIVLHIIGVIGTAGGTG 251
Query: 179 NAIEFFN---VPFSFDERLTLTNFSVEANAKTAII------------------GEEYE-- 215
IEF S + R+++ N S+EA A+ ++ G +++
Sbjct: 252 CVIEFCGDTIAALSMESRMSICNMSIEAGARAGMVAPDEVTFEYLRDKPLAPKGADWDRA 311
Query: 216 -----------GGGYSDEITIELDSLPPMVAKPFSPANGV----------SVED------ 248
Y + I + P + SP + V +ED
Sbjct: 312 VKYWKSLSSDADAKYDINVEINAADIAPTLTWGTSPQDVVPITGSTPDPAKIEDPIRRSA 371
Query: 249 ---------------VEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQVN--VRTLVAPA 291
+EG K+D+VFIGSCTN R E ++ AA ++ G++ V +V P
Sbjct: 372 VQRALDYIGIAPNTPMEGVKVDKVFIGSCTNSRIEDLRAAAAVVKGKRAAEWVDAMVVPG 431
Query: 292 SINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGS 351
S V R+ EGL +I DAG GC CLG + E ST+NRNF GR G+
Sbjct: 432 SGLVKRQAEREGLDKIFTDAGFDWREAGCSMCLGMNPDQLKPGERCASTSNRNFEGRQGA 491
Query: 352 PKAEIYLASPVTAAVSALYGEITN 375
+L SP AA + + G +T+
Sbjct: 492 -GGRTHLVSPAMAAAAGIKGCLTD 514
>gb|AAG31379.1| (AF197448) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 443
Score = 122 bits (304), Expect = 5e-27
Identities = 109/417 (26%), Positives = 177/417 (42%), Gaps = 92/417 (22%)
Query: 20 VDLVYAHDGTMPLIIEAFNRIFNEVRT-----RAYIFFDHVYPAPTVKVANLH------- 67
+DL H+ T P +AF+ + N+ RT + + DH + K+ N+
Sbjct: 27 IDLHLLHEVTSP---QAFDSLRNKQRTVRQSKKTFATMDHNV---STKIQNISASGSMAQ 80
Query: 68 ---REIREFAKIHGIPVVE----GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVF 120
++ + + IP+ + +GI H + E G+ IV GDSHT T GA G
Sbjct: 81 KQMEQLIQNCQEFNIPLYDINNPHQGIVHVIAPEKGMTLPGMTIVCGDSHTSTHGAFGAL 140
Query: 121 AVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNA 180
A G+G ++V L + K +++ + + G ++ A D+++ +I L + +
Sbjct: 141 AFGIGTSEVEHVLATQTLKQKRFKNMKIEIIGKIPRFVTAKDIILFIIGKLGSSSGTGHV 200
Query: 181 IEFFNVPF---SFDERLTLTNFSVEANAKTAIIGEE-----------YEGGG-------- 218
IEF S +ER+T+ N ++E AK+ +I + Y G
Sbjct: 201 IEFCGDVIRNMSMEERMTICNMAIEMGAKSGLIAPDEITYSYLKNKIYSPSGLFWQKSLD 260
Query: 219 ------------YSDEITIELDSLPPMVAKPFSPANGVSVE-------DVEGT------- 252
Y T+ + +L P + + +S++ DV T
Sbjct: 261 YWKLLKSDHNAHYDKCFTVNISNLAPQITWGTNLDQVISIDEKIPDFNDVNSTIKKDSAK 320
Query: 253 -----------------KIDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASI 293
ID+VFIGSCTN R E ++ AA IL +++ +V+ +V P S
Sbjct: 321 SACEYMGLKSNTYLTNISIDKVFIGSCTNARIEDLRSAAGILKNKKIAKHVQAIVVPGSG 380
Query: 294 NVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMG 350
+V R+ EGL +I ID+G PGC CLG + E S +NRNF GR G
Sbjct: 381 SVKRQAEREGLDKIFIDSGFEWRLPGCSMCLGMNKDRLNFGERCASXSNRNFEGRQG 437
>sp|Q00464|LEU2_CANMA 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMALATE ISOMERASE)
(ALPHA-IPM ISOMERASE) (IPMI) >gi|1399939|gb|AAB03335.1|
(U60167) alpha isopropylmalate isomerase [Candida
maltosa]
Length = 770
Score = 120 bits (299), Expect = 2e-26
Identities = 101/383 (26%), Positives = 165/383 (42%), Gaps = 69/383 (18%)
Query: 61 VKVANLHREIREFAKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVF 120
++V L + +++F + + +GI H + E G +V GDSHT T GA G
Sbjct: 88 LQVQTLEQNVKDFDVTYFGMTDDRQGIVHVVGPEQGFTLPGTTVVCGDSHTSTHGAFGAL 147
Query: 121 AVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNA 180
A G+G ++V L P+++ + +DG E I + D+++H+I + +
Sbjct: 148 AFGIGTSEVEHVLATQTTIQANPKNMRITIDGELSEGITSKDLVLHVIGVIGTAGGTGCV 207
Query: 181 IEFFNVPF---SFDERLTLTNFSVEANAKTAII------------------GEEYE---- 215
IEF S + R+++ N ++EA A+ +I G+E+E
Sbjct: 208 IEFAGKAIEDLSMEARMSICNMAIEAGARAGMIKPDEKTFEYIKGRPLAPKGDEWEKALK 267
Query: 216 ---------GGGYSDEITIELDSLPPMVAKPFSPANGV----SVEDVEGT---------- 252
G +I I + P + SP + + SV D
Sbjct: 268 YWKTLHTDDGAKLHYDIKIAASDIVPTITWGNSPQDALPITASVPDPANVSDPIKKSGME 327
Query: 253 -----------------KIDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVR-TLVAPAS 292
K+ + FIGSCTN R E ++ AA++ G + NV+ LV P S
Sbjct: 328 RALKYQGLTPNTPFVVIKMHKAFIGSCTNSRIEDLRAAAKVAKGHKKADNVKLVLVVPGS 387
Query: 293 INVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSP 352
+ ++ +EGL +I AG GC CLG + + E ST+NRNF GR G+
Sbjct: 388 GLIKKQAEKEGLDKIFESAGFTWREAGCSMCLGMNPDILDPEERCASTSNRNFEGRQGA- 446
Query: 353 KAEIYLASPVTAAVSALYGEITN 375
++ +L SP AA +A+ G T+
Sbjct: 447 RSRTHLMSPAMAAAAAIKGHFTD 469
>sp|P55251|LEU2_RHIPU 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMALATE ISOMERASE)
(ALPHA-IPM ISOMERASE) (IPMI) >gi|1000692|dbj|BAA11052.1|
(D67033) LeuA [Rhizomucor pusillus]
Length = 755
Score = 119 bits (296), Expect = 5e-26
Identities = 102/377 (27%), Positives = 158/377 (41%), Gaps = 68/377 (18%)
Query: 66 LHREIREFAKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMG 125
L ++EF + +GI H + E G + GDSHT T GA G A G+G
Sbjct: 92 LEHNVKEFGLTYFGMDDSRQGIVHVIGPEQGFTLPGCTVNCGDSHTSTHGAFGALAFGIG 151
Query: 126 ATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFN 185
++V L + K +++ V +DG + + D+++H+I + + IEF
Sbjct: 152 TSEVEHVLATQTLLQKKSKNMRVKVDGKLAPGVTSKDIVLHIIGVIGTAGGTGCVIEFCG 211
Query: 186 VPF---SFDERLTLTNFSVEANAKTAII------------------GEEYE--------- 215
F S + R+++ N S+EA A+ +I G E++
Sbjct: 212 SAFEQMSMEARMSVCNMSIEAGARAGMIAPDETTFEYIRGRPLAPTGAEWDKAVEYWRSL 271
Query: 216 ----GGGYSDEITIELDSLPPMVAKPFSPANGVSVED----------------------- 248
Y ++ I+ + P + SP + V++
Sbjct: 272 RSDPDAKYDVDVFIDAADIAPTLTWGTSPQDVVAITGTTPDPSTVSDPIRRQAMERALDY 331
Query: 249 --------VEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQVN--VRTLVAPASINVYRR 298
++ KID+VFIGSCTN R E ++ AA I G V V +V P S V ++
Sbjct: 332 IGLKPNTPMQEVKIDKVFIGSCTNSRIEDLRAAAAIAKGRHVADWVYAMVVPGSGLVKKQ 391
Query: 299 MIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYL 358
+EGL RI +AG GC CLG + E ST+NRNF GR G+ +L
Sbjct: 392 AEQEGLDRIFKEAGFDWREAGCSMCLGMNPDQLKPGERCASTSNRNFEGRQGA-GGRTHL 450
Query: 359 ASPVTAAVSALYGEITN 375
SP AA +A+ G T+
Sbjct: 451 MSPAMAAAAAVTGYFTD 467
>pir||G83255 3-isopropylmalate dehydratase large subunit PA3121 [imported] -
Pseudomonas aeruginosa (strain PAO1)
>gi|9949233|gb|AAG06509.1|AE004736_6 (AE004736)
3-isopropylmalate dehydratase large subunit [Pseudomonas
aeruginosa]
Length = 474
Score = 119 bits (296), Expect = 5e-26
Identities = 98/355 (27%), Positives = 150/355 (41%), Gaps = 69/355 (19%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + E G +V GDSHT T GA G A G+G ++V L + K +
Sbjct: 111 QGIVHVVGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLVAKKMK 170
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNVPF---SFDERLTLTNFSV 201
++ V ++G + A D+++ +I + + +AIEF S + R+T+ N S+
Sbjct: 171 NMLVKVEGRLPAGVTAKDIVLAVIGRIGTAGGNGHAIEFAGSAIRDLSIEGRMTICNMSI 230
Query: 202 EANAKTAIIGEEYE---------------------------------------------- 215
EA A+ ++ + +
Sbjct: 231 EAGARVGLVAVDQKTIDYVKGRPFAPSAEQWDQAVACWQGLVSDADARFDTVVELDAAQI 290
Query: 216 ----GGGYSDEITIELDSLPPMVAKPFSPANGVSVED------------VEGTKIDQVFI 259
G S E+ + +D P A+ P S+E + ++D+VFI
Sbjct: 291 KPQVSWGTSPEMVLAVDQNVPDPARESDPIKRGSIERALKYMGLRPNQAITDIQLDRVFI 350
Query: 260 GSCTNGRFEQIKRAAEILDGEQVNV---RTLVAPASINVYRRMIEEGLVRILIDAGVVIL 316
GSCTN R E ++ AAE+ G +V + LV P S V + +EGL RI I+AG
Sbjct: 351 GSCTNSRIEDLRAAAEVARGRKVAATIKQALVVPGSGLVKEQAEKEGLDRIFIEAGFEWR 410
Query: 317 PPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYG 371
PGC CL + E ST+NRNF GR G+ +L SP AA +A+ G
Sbjct: 411 EPGCSMCLAMNPDRLESGEHCASTSNRNFEGRQGA-GGRTHLVSPAMAAAAAVNG 464
>gi|6321429 isopropylmalate isomerase; Leu1p [Saccharomyces cerevisiae]
>gi|1708801|sp|P07264|LEU2_YEAST 3-ISOPROPYLMALATE
DEHYDRATASE (ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM
ISOMERASE) (IPMI) >gi|2131159|pir||S64011
3-isopropylmalate dehydratase (EC 4.2.1.33) - yeast
(Saccharomyces cerevisiae) >gi|1322463|emb|CAA96709.1|
(Z72531) ORF YGL009c [Saccharomyces cerevisiae]
Length = 779
Score = 117 bits (290), Expect = 2e-25
Identities = 102/387 (26%), Positives = 169/387 (43%), Gaps = 77/387 (19%)
Query: 61 VKVANLHREIREFAKIHGIPVV----EGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGA 116
++V L +++F G+P +GI H + E G +V GDSHT T GA
Sbjct: 94 LQVKTLENNVKQF----GVPYFGMSDARQGIVHTIGPEEGFTLPGTTVVCGDSHTSTHGA 149
Query: 117 LGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREM 176
G A G+G ++V L I +++ + ++G I + D+++++I +
Sbjct: 150 FGSLAFGIGTSEVEHVLATQTIIQAKSKNMRITVNGKLSPGITSKDLILYIIGLIGTAGG 209
Query: 177 SYNAIEFFNVPF---SFDERLTLTNFSVEANAKTAII------------------GEEYE 215
+ IEF S + R+++ N ++EA A+ +I G E+E
Sbjct: 210 TGCVIEFAGEAIEALSMEARMSMCNMAIEAGARAGMIKPDETTFQYTKGRPLAPKGAEWE 269
Query: 216 -------------GGGYSDEITIELDSLPPMVAKPFSPANGV----SVED---------- 248
G + EI IE + P + SP + + SV D
Sbjct: 270 KAVAYWKTLKTDEGAKFDHEINIEAVDVIPTITWGTSPQDALPITGSVPDPKNVTDPIKK 329
Query: 249 -----------------VEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVR-TLV 288
++ K+D+VFIGSCTNGR E ++ AA ++ G+++ N++ +V
Sbjct: 330 SGMERALAYMGLEPNTPLKSIKVDKVFIGSCTNGRIEDLRSAAAVVRGQKLASNIKLAMV 389
Query: 289 APASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGR 348
P S V ++ EGL +I +AG GC CLG + + E ST+NRNF GR
Sbjct: 390 VPGSGLVKKQAEAEGLDKIFQEAGFEWREAGCSICLGMNPDILDAYERCASTSNRNFEGR 449
Query: 349 MGSPKAEIYLASPVTAAVSALYGEITN 375
G+ + +L SP AA + + G +
Sbjct: 450 QGA-LSRTHLMSPAMAAAAGIAGHFVD 475
>gi|6323335 Aconitase, mitochondrial; Aco1p [Saccharomyces cerevisiae]
>gi|1168308|sp|P19414|ACON_YEAST ACONITATE HYDRATASE,
MITOCHONDRIAL PRECURSOR (CITRATE HYDRO-LYASE)
(ACONITASE) >gi|1077349|pir||S50387 aconitate hydratase
(EC 4.2.1.3) precursor - yeast (Saccharomyces
cerevisiae) >gi|596052|gb|AAB67348.1| (U17243) Aconitate
hydratase [Saccharomyces cerevisiae]
Length = 778
Score = 116 bits (288), Expect = 4e-25
Identities = 107/415 (25%), Positives = 176/415 (41%), Gaps = 64/415 (15%)
Query: 21 DLVYAHDGTMPL-IIEAFNRIFNEVRTRAYIFFDHVYPAPT------VKVANLHREIREF 73
D V D T + I++ + +V + DH+ A + +L++E+ +F
Sbjct: 90 DRVACQDATAQMAILQFMSAGLPQVAKPVTVHCDHLIQAQVGGEKDLKRAIDLNKEVYDF 149
Query: 74 -----AKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATD 128
AK + G GI HQ+V+E A +I+G DSHTP G LG A+G+G D
Sbjct: 150 LASATAKYNMGFWKPGSGIIHQIVLE-NYAFPGALIIGTDSHTPNAGGLGQLAIGVGGAD 208
Query: 129 VAIALGLGKIW-LKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV- 186
A+ + G+ W LK P+ + V L G + D+++ L + + +E+F
Sbjct: 209 -AVDVMAGRPWELKAPKILGVKLTGKMNGWTSPKDIILKLAGITTVKGGTGKIVEYFGDG 267
Query: 187 --PFSFDERLTLTNFSVEANAKTAIIG------EEYEGGG-------------------- 218
FS T+ N E A T++ E E G
Sbjct: 268 VDTFSATGMGTICNMGAEIGATTSVFPFNKSMIEYLEATGRGKIADFAKLYHKDLLSADK 327
Query: 219 ---YSDEITIELDSLPPMVAKPFSPANGVSVEDVEGTKIDQVF--------IGSCTNGRF 267
Y + + I+L++L P + PF+P V ++ + + IGSCTN +
Sbjct: 328 DAEYDEVVEIDLNTLEPYINGPFTPDLATPVSKMKEVAVANNWPLDVRVGLIGSCTNSSY 387
Query: 268 EQIKRAAEIL-----DGEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGP 322
E + R+A I+ G + V P S + + +G + + G ++L CGP
Sbjct: 388 EDMSRSASIVKDAAAHGLKSKTIFTVTPGSEQIRATIERDGQLETFKEFGGIVLANACGP 447
Query: 323 CLG---RHMGVAGDNEVILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEI 373
C+G R GD I+S+ NRNF R G+P+ ++ASP A+ G++
Sbjct: 448 CIGQWDRRDIKKGDKNTIVSSYNRNFTSRNDGNPQTHAFVASPELVTAFAIAGDL 502
>gb|AAA34389.1| (M33131) aconitase (ACO1) (EC 4.2.1.3) [Saccharomyces cerevisiae]
Length = 779
Score = 116 bits (288), Expect = 4e-25
Identities = 107/415 (25%), Positives = 176/415 (41%), Gaps = 64/415 (15%)
Query: 21 DLVYAHDGTMPL-IIEAFNRIFNEVRTRAYIFFDHVYPAPT------VKVANLHREIREF 73
D V D T + I++ + +V + DH+ A + +L++E+ +F
Sbjct: 90 DRVACQDATAQMAILQFMSAGLPQVAKPVTVHCDHLIQAQVGGEKDLKRAIDLNKEVYDF 149
Query: 74 -----AKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATD 128
AK + G GI HQ+V+E A +I+G DSHTP G LG A+G+G D
Sbjct: 150 LASATAKYNMGFWKPGSGIIHQIVLE-NYAFPGALIIGTDSHTPNAGGLGQLAIGVGGAD 208
Query: 129 VAIALGLGKIW-LKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV- 186
A+ + G+ W LK P+ + V L G + D+++ L + + +E+F
Sbjct: 209 -AVDVMAGRPWELKAPKILGVKLTGKMNGWTSPKDIILKLAGITTVKGGTGKIVEYFGDG 267
Query: 187 --PFSFDERLTLTNFSVEANAKTAIIG------EEYEGGG-------------------- 218
FS T+ N E A T++ E E G
Sbjct: 268 VDTFSATGMGTICNMGAEIGATTSVFPFNKSMIEYLEATGRGKIADFAKLYHKDLLSADK 327
Query: 219 ---YSDEITIELDSLPPMVAKPFSPANGVSVEDVEGTKIDQVF--------IGSCTNGRF 267
Y + + I+L++L P + PF+P V ++ + + IGSCTN +
Sbjct: 328 DAEYDEVVEIDLNTLEPYINGPFTPDLATPVSKMKEVAVANNWPLDVRVGLIGSCTNSSY 387
Query: 268 EQIKRAAEIL-----DGEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGP 322
E + R+A I+ G + V P S + + +G + + G ++L CGP
Sbjct: 388 EDMSRSASIVKDAAAHGLKSKTIFTVTPGSEQIRATIERDGQLETFKEFGGIVLANACGP 447
Query: 323 CLG---RHMGVAGDNEVILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEI 373
C+G R GD I+S+ NRNF R G+P+ ++ASP A+ G++
Sbjct: 448 CIGQWDRRDIKKGDKNTIVSSYNRNFTSRNDGNPQTHAFVASPELVTAFAIAGDL 502
>emb|CAB99455.1| (AJ278693) 3-isopropylmalate dehydratase [Yarrowia lipolytica]
Length = 711
Score = 116 bits (288), Expect = 4e-25
Identities = 109/443 (24%), Positives = 179/443 (39%), Gaps = 88/443 (19%)
Query: 20 VDLVYAHDGTMPLIIEAFNRIFNEVRTR--AYIFFDHVYPAPTVK--------------- 62
+D H+ T P E +VR + DH P T K
Sbjct: 26 IDRHLVHEVTSPQAFEGLKNAGRQVRRKDCTLATVDHNIPTTTRKDFKSIGTFIKEEDSR 85
Query: 63 --VANLHREIREFAKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVF 120
L + +++F + + +GI H + E G +V GDSHT T GA G
Sbjct: 86 LQCTTLEQNVKDFGVRYFGMDDKRQGIVHVVGPEQGFTLPGTTVVCGDSHTSTHGAFGAL 145
Query: 121 AVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNA 180
A G+G ++V L I +++ + + G + I + D+++H+I + +
Sbjct: 146 AFGIGTSEVEHVLATQTIIQAKSKNMRINVSGKLQPGITSKDLILHIIGVIGTAGGTGTV 205
Query: 181 IEFFNVPF---SFDERLTLTNFSVEANAKTAII------------------GEEYE---- 215
IEF S + R+++ N ++E A+ +I G E+E
Sbjct: 206 IEFCGEAIRDLSMEARMSMCNMAIEGGARAGMIAPDETTFRYIRGRPLAPEGAEWEKAVT 265
Query: 216 ---------GGGYSDEITIELDSLPPMVAKPFSPANGV----SVED-------------- 248
+ ++ I+ + + P V SP + + SV D
Sbjct: 266 YWKTLHSDSDAEFDIDVNIKGEDIIPTVTWGTSPQDALPITGSVPDPSKESDPIKRAGIE 325
Query: 249 -------------VEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQVN---VRTLVAPAS 292
+ +D+VFIGSCTN R E ++ AA I+ G++ VR +V P S
Sbjct: 326 RALEYMGLEANTPLNEIAVDKVFIGSCTNSRIEDLRAAAAIVRGKKKADSVVRAMVVPGS 385
Query: 293 INVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSP 352
V R+ EGL ++ AG GC CLG + + E ST+NRNF GR G+
Sbjct: 386 GLVKRKAEAEGLDKVFEAAGFEWREAGCSMCLGMNPDILNPRERCASTSNRNFEGRQGA- 444
Query: 353 KAEIYLASPVTAAVSALYGEITN 375
+ +L SP AA++ + G++T+
Sbjct: 445 LSRTHLVSPAMAALAGITGKLTD 467
>pir||H82564 3-isopropylmalate dehydratase large subunit XF2375 [imported] -
Xylella fastidiosa (strain 9a5c)
>gi|9107553|gb|AAF85174.1|AE004047_5 (AE004047)
3-isopropylmalate dehydratase large subunit [Xylella
fastidiosa]
Length = 474
Score = 115 bits (286), Expect = 7e-25
Identities = 95/359 (26%), Positives = 151/359 (41%), Gaps = 69/359 (19%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + E G +V GDSHT T GA G A G+G ++V L + + +
Sbjct: 111 QGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLVTQKMK 170
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNVPF---SFDERLTLTNFSV 201
++ V ++G+ + A D+++ LI + + A+EF S + R+T+ N ++
Sbjct: 171 NMQVRVEGTLPWGVTAKDIVLALIGKIGTAGGNGYAVEFSGSTIRALSMEGRMTICNMAI 230
Query: 202 EANAKTAII------------------GEEYEGG-------------------------- 217
EA A+ ++ G +++
Sbjct: 231 EAGARVGMVAVDEKTIQYVHGRPFAPKGSDWDAAVAFWRGLVSDPDAHFDRVVELSAEEI 290
Query: 218 ------GYSDEITIELDSLPPMVAKPFSPANGVSV------------EDVEGTKIDQVFI 259
G S E+ +D P + P S+ + + K+D+VFI
Sbjct: 291 KPQVTWGTSPEMVSAVDQSVPDPERETDPVKKESLIRALKYMGLQPNDPITSIKLDRVFI 350
Query: 260 GSCTNGRFEQIKRAAEILDGEQVNV---RTLVAPASINVYRRMIEEGLVRILIDAGVVIL 316
GSCTN R E ++ AAE++ G +V + +V P S V + EGL +I I+AG
Sbjct: 351 GSCTNSRIEDLRAAAEVVKGRKVASTVKQAMVVPGSGLVKAQAEVEGLDKIFIEAGFEWR 410
Query: 317 PPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITN 375
PGC CL + G E ST+NRNF GR G +L SP AA +A+ G +
Sbjct: 411 EPGCSMCLAMNPDKLGSGEHCASTSNRNFEGRQGI-GGRTHLVSPAMAAAAAVAGHFVD 468
>emb|CAC18623.1| (AL451109) related to aconitate hydratase precursor [Neurospora
crassa]
Length = 809
Score = 115 bits (285), Expect = 9e-25
Identities = 99/355 (27%), Positives = 150/355 (41%), Gaps = 64/355 (18%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ V+E A +++G DSH+P G L +G+G D AL +G W LK
Sbjct: 183 GAGIIHQTVLE-NYAVPGLMMLGTDSHSPNAGGLTTITIGVGGADAVEAL-VGAPWELKA 240
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P+ + V L G +++ A D+++HL L R + +E+F S TL N
Sbjct: 241 PKILGVRLTGKLNDWVSAKDIILHLTGRLTVRGGTGYIVEYFGPGIESLSCSGSSTLCNM 300
Query: 200 SVEANAKTAII--------------------------------GEEY-------EGGGYS 220
E A T+I G++ EG Y
Sbjct: 301 GAEIGATTSIFPFTEASSRYLKATNREQADKDAKNFENFTKGTGQDSFFKFKADEGAQYD 360
Query: 221 DEITIELDSLPPMVAKPFSPANGVSVEDVEGT--------KIDQVFIGSCTNGRFEQIKR 272
+ I I+L L P + PF+P + + + + T K+ IGSCTN +E + R
Sbjct: 361 ELIEIDLSKLEPHINGPFTPDLSIPLSEFKKTVQEQKWPEKLSAGLIGSCTNSSYEDMTR 420
Query: 273 AAEILDG-EQVNVRTL----VAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRH 327
I+ EQ ++ + P S + + + ++ AG ++L CGPC+G+
Sbjct: 421 CESIVKAAEQAGIKPASDFYITPGSEGIRATLERDNTLQTFEKAGGIVLSNACGPCIGQW 480
Query: 328 MG----VAGDNEVILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEIT-NP 376
G IL++ NRNFRGR G+ + +LASP A G T NP
Sbjct: 481 KRQDDIPKGTPNAILTSYNRNFRGRNDGNLETMNFLASPEIVTAMAYAGSTTFNP 535
>emb|CAA76360.1| (Y16747) aconitate hydratase [Piromyces sp. E2]
Length = 755
Score = 115 bits (285), Expect = 9e-25
Identities = 98/353 (27%), Positives = 156/353 (43%), Gaps = 60/353 (16%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ+V+E A +++G DSHTP G LG+ AVG+G D A+ + G W LK
Sbjct: 142 GSGIIHQIVLE-NYAYPGGMMIGTDSHTPNAGGLGMIAVGVGGAD-AVDVMAGIPWELKC 199
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFN---VPFSFDERLTLTNF 199
P+ I V L G + +D+++ + L + + +E+F S T+ N
Sbjct: 200 PKIIGVKLTGKRNGWTSPTDIILKVAGILTVKGGTGAIVEYFGEGVESISCTGMATICNM 259
Query: 200 SVEANAKTAIIG-----EEY-----------------------EGGGYSDEITIELDSLP 231
E A T++ EEY EG Y I I L L
Sbjct: 260 GAEIGATTSVFPFNERMEEYLKATGRSEIAELAKQYKDLLKADEGCHYDQIIEINLSELE 319
Query: 232 PMVAKPFSPANGVSVEDVEGT--------KIDQVFIGSCTNGRFEQIKRAAEIL-----D 278
P + PF+P + + + ++ IGSCTN +E + R + ++ +
Sbjct: 320 PHINGPFTPDLATPLSEFKNAVEKNGWPEELKVGLIGSCTNSSYEDMCRVSSLIKQALDN 379
Query: 279 GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNE 335
G + ++ P S + + ++G + ++ G V+L CGPC+G R GD
Sbjct: 380 GVPAKSKFIITPGSEQIRATIAKDGQMELMKKVGGVVLANACGPCIGQWDRKDIKKGDLN 439
Query: 336 VILSTTNRNFRGRM-GSPKAEIYLASP--VTAAV-------SALYGEITNPEG 378
I+++ NRNF R +P+ ++ASP VTA V + + EIT P+G
Sbjct: 440 SIITSYNRNFTARNDANPQTHAFVASPEIVTAMVFGGSLKFNPMKDEITKPDG 492
>sp|P34455|ACON_CAEEL PROBABLE ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSOR (CITRATE
HYDRO-LYASE) (ACONITASE) >gi|630644|pir||S44831 F54H12.1
protein - Caenorhabditis elegans
>gi|409297|gb|AAA28050.1| (L25599) aconitate hydratase
[Caenorhabditis elegans]
Length = 788
Score = 114 bits (283), Expect = 2e-24
Identities = 97/338 (28%), Positives = 145/338 (42%), Gaps = 49/338 (14%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVP 143
G GI HQ+++E A +++G DSHTP G LG +G+G D + LK P
Sbjct: 177 GSGIIHQIILE-NYAFPGLLLIGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCP 235
Query: 144 ESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNFS 200
+ I + L G + A DV++ + L + + +E+F S T+ N
Sbjct: 236 KVIGIKLTGKLNGWTSAKDVILKVADILTVKGGTGAIVEYFGPGVDSISATGMGTICNMG 295
Query: 201 VEANAKTAI------------------IGEEY----------EGGGYSDEITIELDSLPP 232
E A T++ I EE +G Y I I LD+L P
Sbjct: 296 AEIGATTSVFPYNESMYKYLEATGRKEIAEEARKYKDLLTADDGANYDQIIEINLDTLTP 355
Query: 233 MVAKPFSPANGVSVEDV-EGTK-------IDQVFIGSCTNGRFEQIKRAAEI----LD-G 279
V PF+P S++ + E K + IGSCTN +E + RAA I LD G
Sbjct: 356 HVNGPFTPDLASSIDKLGENAKKNGWPLDVKVSLIGSCTNSSYEDMTRAASIAKQALDKG 415
Query: 280 EQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNEV 336
+ + P S V + +GL +I D G ++L CGPC+G R G+
Sbjct: 416 LKAKTIFTITPGSEQVRATIERDGLSKIFADFGGMVLANACGPCIGQWDRQDVKKGEKNT 475
Query: 337 ILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEI 373
I+++ NRNF GR +P ++ SP A+ G +
Sbjct: 476 IVTSYNRNFTGRNDANPATHGFVTSPDITTAMAISGRL 513
>sp|P20004|ACON_BOVIN ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSOR (CITRATE HYDRO-LYASE)
(ACONITASE) >gi|1364216|emb|CAA90177.1| (Z49931)
aconitase [Bos taurus]
Length = 780
Score = 114 bits (282), Expect = 2e-24
Identities = 98/344 (28%), Positives = 151/344 (43%), Gaps = 52/344 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ+++E A +++G DSHTP G LG +G+G D A+ + G W LK
Sbjct: 169 GSGIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVTAGIPWELKC 226
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P+ I V L GS + DV++ + L + + +E+ S T+ N
Sbjct: 227 PKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNM 286
Query: 200 SVEANAKTAI---------------------IGEEYE-------GGGYSDEITIELDSLP 231
E A T++ + +E++ G Y I I L L
Sbjct: 287 GAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPDSGCHYDQLIEINLSELK 346
Query: 232 PMVAKPFSP------ANGVSVEDVEGTKID--QVFIGSCTNGRFEQIKRAAEILD----- 278
P + PF+P A SV + EG +D IGSCTN +E + R+A +
Sbjct: 347 PHINGPFTPDLAHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAH 406
Query: 279 GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNE 335
G Q + + P S + + +G +IL D G ++L CGPC+G R G+
Sbjct: 407 GLQCKSQFTITPGSEQIRATIERDGYAQILRDVGGIVLANACGPCIGQWDRKDIKKGEKN 466
Query: 336 VILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEIT-NPE 377
I+++ NRNF GR +P+ ++ SP A+ G + NPE
Sbjct: 467 TIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPE 510
>sp|P15717|LEU2_SALTY 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
Length = 465
Score = 114 bits (282), Expect = 2e-24
Identities = 113/462 (24%), Positives = 180/462 (38%), Gaps = 93/462 (20%)
Query: 2 TLVGKLLNAK-----PGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRT--RAYIFFDH 54
TL KL +A P E + +D H+ T P + + VR + + DH
Sbjct: 4 TLYEKLFDAHVVFEAPNETPLLYIDRHLVHEVTSPQAFDGLRAHPSPVRQPGKTFATMDH 63
Query: 55 VYPAPT-----------VKVANLHREIREFAKIHGIPVVE----GKGISHQLVVELGLAE 99
T +++ L + EF G+ + + +GI H + E G+
Sbjct: 64 NVSTQTKDINASGEMARIQMQELIKNCNEF----GVELYDLNHPYQGIVHVMGPEQGVTL 119
Query: 100 EAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIM 159
IV GDSHT T GA G A G+G ++V L + +++ + + G+ I
Sbjct: 120 PGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVTGNAAPGIT 179
Query: 160 ASDVMIHLITSLKDREMSYNAIEFFNVPF---SFDERLTLTNFSVEANAKTAIIGE---- 212
A D+++ +I + + +EF S + R+TL N ++E AK ++
Sbjct: 180 AKDIVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVAPDETT 239
Query: 213 --------------------EY-------EGGGYSDEITIELDSLPPMVAKPFSPANGVS 245
EY +G + + + + + P V +P +S
Sbjct: 240 FNYVKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVALRAEEIAPQVTWGTNPGQVIS 299
Query: 246 VEDV-------------------------------EGTKIDQVFIGSCTNGRFEQIKRAA 274
V D+ ID+VFIG CTN R E ++ A
Sbjct: 300 VTDIIPDPASFSDPVERASAEKALAYMGLQPGVPLTDVAIDKVFIGPCTNSRIEDLRAPA 359
Query: 275 EILDGEQV-NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGD 333
E+ G ++ V+ LV P S V + EGL +I I+AG PGC C+ +
Sbjct: 360 EVGKGPKLPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCVAVNNDRLNP 419
Query: 334 NEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITN 375
E ST+NRNF GR G +L SP AA +A+ G +
Sbjct: 420 GERCASTSNRNFEGRQGR-GGRTHLVSPAMAAAAAVTGHFAD 460
>gb|AAB19612.1| (S57886) isopropylmalate isomerase {EC 4.2.1.33} [Saccharomyces
cerevisiae, Peptide, 782 aa]
Length = 782
Score = 114 bits (282), Expect = 2e-24
Identities = 103/390 (26%), Positives = 170/390 (43%), Gaps = 80/390 (20%)
Query: 61 VKVANLHREIREFAKIHGIPVV----EGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGA 116
++V L +++F G+P +GI H + E G +V GDSHT T GA
Sbjct: 94 LQVKTLENNVKQF----GVPYFGMSDARQGIVHTIGPEEGFTLPGTTVVCGDSHTSTHGA 149
Query: 117 LGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREM 176
G A G+G ++V L I +++ + ++G I + D+++++I +
Sbjct: 150 FGSLAFGIGTSEVEHVLATQTIIQAKSKNMRITVNGKLSPGITSKDLILYIIGLIGTAGG 209
Query: 177 SYNAIEFFNVPF---SFDERLTLTNFSVEANAKTAII------------------GEEYE 215
+ IEF S + R+++ N ++EA A+ +I G E+E
Sbjct: 210 TGCVIEFAGEAIEALSMEARMSMCNMAIEAGARAGMIKPDETTFQYTKGRPLAPKGAEWE 269
Query: 216 -------------GGGYSDEIT---IELDSLPPMVAKPFSPANGV----SVED------- 248
G + EIT IE + P + SP + + SV D
Sbjct: 270 KAVAYWKTLKTDEGAKFDHEITLKHIEAVDVIPTITWGTSPQDALPITGSVPDPKNVTDP 329
Query: 249 --------------------VEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVR- 285
++ K+D+VFIGSCTNGR E ++ AA ++ G+++ N++
Sbjct: 330 IKKSGMERALAYMGLEPNTPLKSIKVDKVFIGSCTNGRIEDLRSAAAVVRGQKLASNIKL 389
Query: 286 TLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNF 345
+V P S V ++ EGL +I +AG GC CLG + + E ST+NRNF
Sbjct: 390 AMVVPGSGLVKKQAEAEGLDKIFQEAGFEWREAGCSMCLGMNPDILDAYERCASTSNRNF 449
Query: 346 RGRMGSPKAEIYLASPVTAAVSALYGEITN 375
GR G+ + +L SP AA + + G +
Sbjct: 450 EGRQGA-LSRTHLISPAMAAAAGIAGHFVD 478
>pdb|5ACN| Aconitase (E.C.4.2.1.3) (Inactive (3fe-4s) Cluster Form)
>gi|231188|pdb|6ACN| Aconitase (E.C.4.2.1.3) (Activated
(4fe-4s) Cluster Form) >gi|443544|pdb|7ACN| Aconitase
(E.C.4.2.1.3) Complex With Isocitrate
Length = 754
Score = 112 bits (277), Expect = 8e-24
Identities = 96/344 (27%), Positives = 151/344 (42%), Gaps = 52/344 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ+++E A +++G DSHTP G LG +G+G D A+ + G W LK
Sbjct: 142 GSGIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKC 199
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P+ I V L GS + DV++ + L + + +E+ S T+ N
Sbjct: 200 PKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNM 259
Query: 200 SVEANAKTAI---------------------IGEEYE-------GGGYSDEITIELDSLP 231
E A T++ + +E++ G Y I I L L
Sbjct: 260 GAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPDPGCHYDQVIEINLSELK 319
Query: 232 PMVAKPFSP------ANGVSVEDVEGTKID--QVFIGSCTNGRFEQIKRAAEILD----- 278
P + PF+P A SV + EG +D IGSCTN +E + R+A +
Sbjct: 320 PHINGPFTPDLAHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAH 379
Query: 279 GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNE 335
G + + + P S + + +G ++L D G ++L CGPC+G R G+
Sbjct: 380 GLKCKSQFTITPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKN 439
Query: 336 VILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEIT-NPE 377
I+++ NRNF GR +P+ ++ SP A+ G + NPE
Sbjct: 440 TIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPE 483
>pdb|1C96|A Chain A, S642a:citrate Complex Of Aconitase
Length = 753
Score = 112 bits (277), Expect = 8e-24
Identities = 96/344 (27%), Positives = 151/344 (42%), Gaps = 52/344 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ+++E A +++G DSHTP G LG +G+G D A+ + G W LK
Sbjct: 141 GSGIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKC 198
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P+ I V L GS + DV++ + L + + +E+ S T+ N
Sbjct: 199 PKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNM 258
Query: 200 SVEANAKTAI---------------------IGEEYE-------GGGYSDEITIELDSLP 231
E A T++ + +E++ G Y I I L L
Sbjct: 259 GAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPDPGCHYDQVIEINLSELK 318
Query: 232 PMVAKPFSP------ANGVSVEDVEGTKID--QVFIGSCTNGRFEQIKRAAEILD----- 278
P + PF+P A SV + EG +D IGSCTN +E + R+A +
Sbjct: 319 PHINGPFTPDLAHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAH 378
Query: 279 GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNE 335
G + + + P S + + +G ++L D G ++L CGPC+G R G+
Sbjct: 379 GLKCKSQFTITPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKN 438
Query: 336 VILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEIT-NPE 377
I+++ NRNF GR +P+ ++ SP A+ G + NPE
Sbjct: 439 TIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPE 482
>pdb|1C97|A Chain A, S642a:isocitrate Complex Of Aconitase
>gi|6729979|pdb|1B0K|A Chain A, S642a:fluorocitrate
Complex Of Aconitase
Length = 753
Score = 112 bits (277), Expect = 8e-24
Identities = 96/344 (27%), Positives = 151/344 (42%), Gaps = 52/344 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ+++E A +++G DSHTP G LG +G+G D A+ + G W LK
Sbjct: 141 GSGIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKC 198
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P+ I V L GS + DV++ + L + + +E+ S T+ N
Sbjct: 199 PKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNM 258
Query: 200 SVEANAKTAI---------------------IGEEYE-------GGGYSDEITIELDSLP 231
E A T++ + +E++ G Y I I L L
Sbjct: 259 GAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPDPGCHYDQVIEINLSELK 318
Query: 232 PMVAKPFSP------ANGVSVEDVEGTKID--QVFIGSCTNGRFEQIKRAAEILD----- 278
P + PF+P A SV + EG +D IGSCTN +E + R+A +
Sbjct: 319 PHINGPFTPDLAHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAH 378
Query: 279 GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNE 335
G + + + P S + + +G ++L D G ++L CGPC+G R G+
Sbjct: 379 GLKCKSQFTITPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKN 438
Query: 336 VILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEIT-NPE 377
I+++ NRNF GR +P+ ++ SP A+ G + NPE
Sbjct: 439 TIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPE 482
>pdb|1B0J|A Chain A, Crystal Structure Of Aconitase With Isocitrate
Length = 754
Score = 112 bits (277), Expect = 8e-24
Identities = 96/344 (27%), Positives = 151/344 (42%), Gaps = 52/344 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ+++E A +++G DSHTP G LG +G+G D A+ + G W LK
Sbjct: 142 GSGIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKC 199
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P+ I V L GS + DV++ + L + + +E+ S T+ N
Sbjct: 200 PKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNM 259
Query: 200 SVEANAKTAI---------------------IGEEYE-------GGGYSDEITIELDSLP 231
E A T++ + +E++ G Y I I L L
Sbjct: 260 GAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPDPGCHYDQVIEINLSELK 319
Query: 232 PMVAKPFSP------ANGVSVEDVEGTKID--QVFIGSCTNGRFEQIKRAAEILD----- 278
P + PF+P A SV + EG +D IGSCTN +E + R+A +
Sbjct: 320 PHINGPFTPDLAHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAH 379
Query: 279 GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNE 335
G + + + P S + + +G ++L D G ++L CGPC+G R G+
Sbjct: 380 GLKCKSQFTITPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKN 439
Query: 336 VILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEIT-NPE 377
I+++ NRNF GR +P+ ++ SP A+ G + NPE
Sbjct: 440 TIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPE 483
>pdb|1B0M|A Chain A, Aconitase R644q:fluorocitrate Complex
Length = 753
Score = 112 bits (277), Expect = 8e-24
Identities = 96/344 (27%), Positives = 151/344 (42%), Gaps = 52/344 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ+++E A +++G DSHTP G LG +G+G D A+ + G W LK
Sbjct: 141 GSGIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKC 198
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P+ I V L GS + DV++ + L + + +E+ S T+ N
Sbjct: 199 PKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNM 258
Query: 200 SVEANAKTAI---------------------IGEEYE-------GGGYSDEITIELDSLP 231
E A T++ + +E++ G Y I I L L
Sbjct: 259 GAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPDPGCHYDQVIEINLSELK 318
Query: 232 PMVAKPFSP------ANGVSVEDVEGTKID--QVFIGSCTNGRFEQIKRAAEILD----- 278
P + PF+P A SV + EG +D IGSCTN +E + R+A +
Sbjct: 319 PHINGPFTPDLAHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAH 378
Query: 279 GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNE 335
G + + + P S + + +G ++L D G ++L CGPC+G R G+
Sbjct: 379 GLKCKSQFTITPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKN 438
Query: 336 VILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEIT-NPE 377
I+++ NRNF GR +P+ ++ SP A+ G + NPE
Sbjct: 439 TIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPE 482
>sp|P16276|ACON_PIG ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSOR (CITRATE HYDRO-LYASE)
(ACONITASE) >gi|108227|pir||A35544 aconitate hydratase
(EC 4.2.1.3) precursor, mitochondrial [validated] - pig
>gi|164315|gb|AAA30987.1| (J05224) heart aconitase
precursor [Sus scrofa]
Length = 781
Score = 112 bits (277), Expect = 8e-24
Identities = 96/344 (27%), Positives = 151/344 (42%), Gaps = 52/344 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ+++E A +++G DSHTP G LG +G+G D A+ + G W LK
Sbjct: 169 GSGIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKC 226
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P+ I V L GS + DV++ + L + + +E+ S T+ N
Sbjct: 227 PKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNM 286
Query: 200 SVEANAKTAI---------------------IGEEYE-------GGGYSDEITIELDSLP 231
E A T++ + +E++ G Y I I L L
Sbjct: 287 GAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPDPGCHYDQVIEINLSELK 346
Query: 232 PMVAKPFSP------ANGVSVEDVEGTKID--QVFIGSCTNGRFEQIKRAAEILD----- 278
P + PF+P A SV + EG +D IGSCTN +E + R+A +
Sbjct: 347 PHINGPFTPDLAHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAH 406
Query: 279 GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNE 335
G + + + P S + + +G ++L D G ++L CGPC+G R G+
Sbjct: 407 GLKCKSQFTITPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKN 466
Query: 336 VILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEIT-NPE 377
I+++ NRNF GR +P+ ++ SP A+ G + NPE
Sbjct: 467 TIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPE 510
>pdb|8ACN| Aconitase (E.C.4.2.1.3) Complex With Nitroisocitrate
>gi|442617|pdb|1ACO| Aconitase (Mitochondrial)
(E.C.4.2.1.3) Complex With Transaconitate
>gi|1942843|pdb|1FGH| Complex With
4-Hydroxy-Trans-Aconitate
Length = 754
Score = 111 bits (276), Expect = 1e-23
Identities = 96/344 (27%), Positives = 151/344 (42%), Gaps = 52/344 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ+++E A +++G DSHTP G LG +G+G D A+ + G W LK
Sbjct: 142 GSGIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKC 199
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P+ I V L GS + DV++ + L + + +E+ S T+ N
Sbjct: 200 PKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNM 259
Query: 200 SVEANAKTAI---------------------IGEEYE-------GGGYSDEITIELDSLP 231
E A T++ + +E++ G Y I I L L
Sbjct: 260 GAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPDSGCHYDQLIEINLSELK 319
Query: 232 PMVAKPFSP------ANGVSVEDVEGTKID--QVFIGSCTNGRFEQIKRAAEILD----- 278
P + PF+P A SV + EG +D IGSCTN +E + R+A +
Sbjct: 320 PHINGPFTPDLAHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAH 379
Query: 279 GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNE 335
G + + + P S + + +G ++L D G ++L CGPC+G R G+
Sbjct: 380 GLKCKSQFTITPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKN 439
Query: 336 VILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEIT-NPE 377
I+++ NRNF GR +P+ ++ SP A+ G + NPE
Sbjct: 440 TIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPE 483
>pdb|1NIS| Aconitase (E.C.4.2.1.3) Complex With Nitrocitrate (Major Occupancy
Form) >gi|443149|pdb|1NIT| Aconitase (E.C.4.2.1.3)
Complex With Sulfate (Minor Occupancy Form)
Length = 754
Score = 111 bits (276), Expect = 1e-23
Identities = 96/344 (27%), Positives = 151/344 (42%), Gaps = 52/344 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ+++E A +++G DSHTP G LG +G+G D A+ + G W LK
Sbjct: 142 GSGIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKC 199
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P+ I V L GS + DV++ + L + + +E+ S T+ N
Sbjct: 200 PKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNM 259
Query: 200 SVEANAKTAI---------------------IGEEYE-------GGGYSDEITIELDSLP 231
E A T++ + +E++ G Y I I L L
Sbjct: 260 GAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPDSGCHYDQLIEINLSELK 319
Query: 232 PMVAKPFSP------ANGVSVEDVEGTKID--QVFIGSCTNGRFEQIKRAAEILD----- 278
P + PF+P A SV + EG +D IGSCTN +E + R+A +
Sbjct: 320 PHINGPFTPDLAHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAH 379
Query: 279 GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNE 335
G + + + P S + + +G ++L D G ++L CGPC+G R G+
Sbjct: 380 GLKCKSQFTITPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKN 439
Query: 336 VILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEIT-NPE 377
I+++ NRNF GR +P+ ++ SP A+ G + NPE
Sbjct: 440 TIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPE 483
>pdb|1AMJ| Aconitase (E.C.4.2.1.3) Complexed With Sulfate And Hydroxide
>gi|999984|pdb|1AMI| Aconitase (E.C.4.2.1.3) Complexed
With Alpha-Methyl-Isocitrate
Length = 754
Score = 111 bits (276), Expect = 1e-23
Identities = 96/344 (27%), Positives = 151/344 (42%), Gaps = 52/344 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ+++E A +++G DSHTP G LG +G+G D A+ + G W LK
Sbjct: 142 GSGIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKC 199
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P+ I V L GS + DV++ + L + + +E+ S T+ N
Sbjct: 200 PKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNM 259
Query: 200 SVEANAKTAI---------------------IGEEYE-------GGGYSDEITIELDSLP 231
E A T++ + +E++ G Y I I L L
Sbjct: 260 GAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPDSGCHYDQLIEINLSELK 319
Query: 232 PMVAKPFSP------ANGVSVEDVEGTKID--QVFIGSCTNGRFEQIKRAAEILD----- 278
P + PF+P A SV + EG +D IGSCTN +E + R+A +
Sbjct: 320 PHINGPFTPDLAHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAH 379
Query: 279 GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNE 335
G + + + P S + + +G ++L D G ++L CGPC+G R G+
Sbjct: 380 GLKCKSQFTITPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKN 439
Query: 336 VILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEIT-NPE 377
I+++ NRNF GR +P+ ++ SP A+ G + NPE
Sbjct: 440 TIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPE 483
>pir||S57528 aconitate hydratase (EC 4.2.1.3) precursor, mitochondrial
[validated] - bovine
Length = 781
Score = 111 bits (276), Expect = 1e-23
Identities = 96/344 (27%), Positives = 151/344 (42%), Gaps = 52/344 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ+++E A +++G DSHTP G LG +G+G D A+ + G W LK
Sbjct: 169 GSGIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKC 226
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P+ I V L GS + DV++ + L + + +E+ S T+ N
Sbjct: 227 PKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNM 286
Query: 200 SVEANAKTAI---------------------IGEEYE-------GGGYSDEITIELDSLP 231
E A T++ + +E++ G Y I I L L
Sbjct: 287 GAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPDSGCHYDQLIEINLSELK 346
Query: 232 PMVAKPFSP------ANGVSVEDVEGTKID--QVFIGSCTNGRFEQIKRAAEILD----- 278
P + PF+P A SV + EG +D IGSCTN +E + R+A +
Sbjct: 347 PHINGPFTPDLAHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAH 406
Query: 279 GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNE 335
G + + + P S + + +G ++L D G ++L CGPC+G R G+
Sbjct: 407 GLKCKSQFTITPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKN 466
Query: 336 VILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEIT-NPE 377
I+++ NRNF GR +P+ ++ SP A+ G + NPE
Sbjct: 467 TIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPE 510
>gb|AAB38416.1| (U80040) mitochondrial aconitase [Homo sapiens]
Length = 780
Score = 110 bits (272), Expect = 3e-23
Identities = 96/344 (27%), Positives = 150/344 (42%), Gaps = 52/344 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ+++E A +++G DSHTP G LG +G+G D A+ + G W LK
Sbjct: 169 GSGIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKC 226
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P+ I V L GS + DV++ + L + + +E+ S T+ N
Sbjct: 227 PKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSMSCTGMATICNM 286
Query: 200 SVEANAKTAI---------------------IGEEYE-------GGGYSDEITIELDSLP 231
E A T++ + +E++ G Y I I L L
Sbjct: 287 GAEIGATTSVFPYNHRMKKYLSKTGREDIANLADEFKDHLVPDPGCHYDQLIEINLSELK 346
Query: 232 PMVAKPFSP------ANGVSVEDVEGTKID--QVFIGSCTNGRFEQIKRAAEILD----- 278
P + PF+P A V + EG +D IGSCTN +E + R+A +
Sbjct: 347 PHINGPFTPDLAHPVAEVGKVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAH 406
Query: 279 GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNE 335
G + + + P S + + +G +IL D G ++L CGPC+G R G+
Sbjct: 407 GLKCKSQFTITPGSEQIRATIERDGYAQILRDLGGIVLANACGPCIGQWDRKDIKKGEKN 466
Query: 336 VILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEIT-NPE 377
I+++ NRNF GR +P+ ++ SP A+ G + NPE
Sbjct: 467 TIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPE 510
>gi|4501867 aconitase 2; Aconitase, mitochondrial [Homo sapiens]
>gi|11418186|ref|XP_010018.1| aconitase 2 [Homo sapiens]
>gi|6686275|sp|Q99798|ACON_HUMAN ACONITATE HYDRATASE,
MITOCHONDRIAL PRECURSOR (CITRATE HYDRO-LYASE)
(ACONITASE) >gi|3366620|gb|AAC39921.1| (U87939)
aconitate hydratase [Homo sapiens]
Length = 780
Score = 110 bits (272), Expect = 3e-23
Identities = 110/428 (25%), Positives = 183/428 (42%), Gaps = 65/428 (15%)
Query: 13 GEAVIR-KVDLVYAHDGTMPL-IIEAFNRIFNEVRTRAYIFFDHVYPAPTV------KVA 64
G++ +R + D V D T + +++ + ++V + I DH+ A +
Sbjct: 85 GKSYLRLRPDRVAMQDATAQMAMLQFISSGLSKVAVPSTIHCDHLIEAQVGGEKDLRRAK 144
Query: 65 NLHREIREFAKIHGIPV-----VEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGV 119
++++E+ F G G GI HQ+++E A +++G DSHTP G LG
Sbjct: 145 DINQEVYNFLATAGAKYGVGFWKPGSGIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGG 203
Query: 120 FAVGMGATDVAIALGLGKIW-LKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSY 178
+G+G D A+ + G W LK P+ I V L GS + DV++ + L + +
Sbjct: 204 ICIGVGGAD-AVDVMAGIPWELKCPKVIGVKLTGSLSGWSSPKDVILKVAGILTVKGGTG 262
Query: 179 NAIEFFNV---PFSFDERLTLTNFSVEANAKTAI---------------------IGEEY 214
+E+ S T+ N E A T++ + +E+
Sbjct: 263 AIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGREDIANLADEF 322
Query: 215 E-------GGGYSDEITIELDSLPPMVAKPFSP------ANGVSVEDVEGTKID--QVFI 259
+ G Y I I L L P + PF+P A V + EG +D I
Sbjct: 323 KDHLVPDPGCHYDQLIEINLSELKPHINGPFTPDLAHPVAEVGKVAEKEGWPLDIRVGLI 382
Query: 260 GSCTNGRFEQIKRAAEILD-----GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVV 314
GSCTN +E + R+A + G + + + P S + + +G +IL D G +
Sbjct: 383 GSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIERDGYAQILRDLGGI 442
Query: 315 ILPPGCGPCLG---RHMGVAGDNEVILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALY 370
+L CGPC+G R G+ I+++ NRNF GR +P+ ++ SP A+
Sbjct: 443 VLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIA 502
Query: 371 GEIT-NPE 377
G + NPE
Sbjct: 503 GTLKFNPE 510
>emb|CAB46022.1| (AL023553) dJ347H13.1 (aconitase 2, mitochondrial (Aconitate
Hydratase, EC 4.2.1.3, Citrate Hydrolyase)) [Homo
sapiens]
Length = 768
Score = 110 bits (272), Expect = 3e-23
Identities = 110/428 (25%), Positives = 183/428 (42%), Gaps = 65/428 (15%)
Query: 13 GEAVIR-KVDLVYAHDGTMPL-IIEAFNRIFNEVRTRAYIFFDHVYPAPTV------KVA 64
G++ +R + D V D T + +++ + ++V + I DH+ A +
Sbjct: 73 GKSYLRLRPDRVAMQDATAQMAMLQFISSGLSKVAVPSTIHCDHLIEAQVGGEKDLRRAK 132
Query: 65 NLHREIREFAKIHGIPV-----VEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGV 119
++++E+ F G G GI HQ+++E A +++G DSHTP G LG
Sbjct: 133 DINQEVYNFLATAGAKYGVGFWKPGSGIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGG 191
Query: 120 FAVGMGATDVAIALGLGKIW-LKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSY 178
+G+G D A+ + G W LK P+ I V L GS + DV++ + L + +
Sbjct: 192 ICIGVGGAD-AVDVMAGIPWELKCPKVIGVKLTGSLSGWSSPKDVILKVAGILTVKGGTG 250
Query: 179 NAIEFFNV---PFSFDERLTLTNFSVEANAKTAI---------------------IGEEY 214
+E+ S T+ N E A T++ + +E+
Sbjct: 251 AIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGREDIANLADEF 310
Query: 215 E-------GGGYSDEITIELDSLPPMVAKPFSP------ANGVSVEDVEGTKID--QVFI 259
+ G Y I I L L P + PF+P A V + EG +D I
Sbjct: 311 KDHLVPDPGCHYDQLIEINLSELKPHINGPFTPDLAHPVAEVGKVAEKEGWPLDIRVGLI 370
Query: 260 GSCTNGRFEQIKRAAEILD-----GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVV 314
GSCTN +E + R+A + G + + + P S + + +G +IL D G +
Sbjct: 371 GSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIERDGYAQILRDLGGI 430
Query: 315 ILPPGCGPCLG---RHMGVAGDNEVILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALY 370
+L CGPC+G R G+ I+++ NRNF GR +P+ ++ SP A+
Sbjct: 431 VLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIA 490
Query: 371 GEIT-NPE 377
G + NPE
Sbjct: 491 GTLKFNPE 498
>gb|AAD19351.2| (AF093096) aconitase [Homo sapiens]
Length = 780
Score = 109 bits (269), Expect = 7e-23
Identities = 96/344 (27%), Positives = 150/344 (42%), Gaps = 52/344 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ+++E A +++G DSHTP G LG +G+G D A+ + G W LK
Sbjct: 169 GSGIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKC 226
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P+ I V L GS + DV++ + L + + +E+ S T+ N
Sbjct: 227 PKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSMSCTGMATICNM 286
Query: 200 SVEANAKTAI---------------------IGEEYE-------GGGYSDEITIELDSLP 231
E A T++ + +E++ G Y I I L L
Sbjct: 287 GAEIGATTSVFPYNHRMKKYLSKTGREDIANLADEFKDHLVPDPGCHYDQLIEINLSELK 346
Query: 232 PMVAKPFSP------ANGVSVEDVEGTKID--QVFIGSCTNGRFEQIKRAAEILD----- 278
P + PF+P A V + EG +D IGSCTN +E + R+A +
Sbjct: 347 PHINGPFTPDLAHPVAEVGKVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAH 406
Query: 279 GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNE 335
G + + + P S + + +G +IL D G ++L CGPC+G R G+
Sbjct: 407 GLKCKSQFTITPGSEQIRATIERDGYRQILRDLGGIVLANACGPCIGQWDRKDIKKGEKN 466
Query: 336 VILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEIT-NPE 377
I+++ NRNF GR +P+ ++ SP A+ G + NPE
Sbjct: 467 TIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPE 510
>emb|CAC11018.1| (AJ243266) mitochondrial aconitase [Rattus norvegicus]
Length = 780
Score = 109 bits (269), Expect = 7e-23
Identities = 96/344 (27%), Positives = 149/344 (42%), Gaps = 52/344 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWL-KV 142
G GI HQ+++E A +++G DSHTP G LG +G+G D A+ + G W K
Sbjct: 169 GSGIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWEPKC 226
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P+ I V L G+ + DV++ + L + + +E+ S T+ N
Sbjct: 227 PKVIGVKLTGTLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNM 286
Query: 200 SVEANAKTAI---------------------IGEEYE-------GGGYSDEITIELDSLP 231
E A T + + EE++ G Y I I L+ L
Sbjct: 287 GAEIGATTPVFPYNHRMKKYLSKTGRADIANLAEEFKDHLVPDPGCQYDQVIEINLNELK 346
Query: 232 PMVAKPFSPANGVSVEDV------EGTKID--QVFIGSCTNGRFEQIKRAAEILD----- 278
P + PF+P V DV EG +D IGSCTN +E + R+A +
Sbjct: 347 PHINGPFTPDLAHPVADVGTVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAH 406
Query: 279 GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNE 335
G + + + P S + + +G +IL D G ++L CGPC+G R G+
Sbjct: 407 GLKCKSQFTITPGSEQIRATIERDGYAQILRDVGGIVLANACGPCIGQWDRKDIKKGEKN 466
Query: 336 VILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEIT-NPE 377
I+++ NRNF GR +P+ ++ SP A+ G + NPE
Sbjct: 467 TIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPE 510
>gb|AAF54588.1| (AE003690) CG4706 gene product [Drosophila melanogaster]
Length = 769
Score = 108 bits (268), Expect = 9e-23
Identities = 112/422 (26%), Positives = 176/422 (41%), Gaps = 62/422 (14%)
Query: 13 GEAVIR-KVDLVYAHDGTMPL-IIEAFNRIFNEVRTRAYIFFDHVYPAPT------VKVA 64
G++ +R + D V D T + +++ + +V + + DH+ A +
Sbjct: 69 GKSYLRLRPDRVALQDATAQMTLLQFISSGLKKVAVPSTVHCDHLIEAQISGDKDLARAK 128
Query: 65 NLHREIREF-----AKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGV 119
+L++E+ +F AK + G GI HQ+++E A +++G DSHTP G LG
Sbjct: 129 DLNKEVYDFLSSACAKYNLGFWKPGSGIIHQIILE-NYAFPGLLMIGTDSHTPNGGGLGC 187
Query: 120 FAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYN 179
VG+G D + LK P I L G + DV++ + L + +
Sbjct: 188 LCVGVGGADAVDVMANIPWELKCPTVIGCHLTGKISGWTSPKDVILKVAEILTVKGGTGA 247
Query: 180 AIEFFN---VPFSFDERLTLTNFSVEANAKTAI------------------IGEEY---- 214
+E+ S T+TN E A T+I I +E
Sbjct: 248 IVEYHGPGVESISCTGMATITNMGAEIGATTSIFPFNERMVTYLRATGRGAIADEATKNK 307
Query: 215 ------EGGGYSDEITIELDSLPPMVAKPFSP--ANGVSVEDVEGTK------IDQVFIG 260
EG Y I I LD+L P+V PF+P A+ +S K I IG
Sbjct: 308 DLLVPDEGCKYDKVIEINLDTLEPLVNGPFTPDLAHPISKLGQNSEKNGYPMEIKVSLIG 367
Query: 261 SCTNGRFEQIKRAAEILD-----GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVI 315
SCTN +E + R A I + G + V V P S V + +G++ +L G +
Sbjct: 368 SCTNSSYEDMGRCASIANDALGHGLKSCVPFNVTPGSEQVRATIARDGIIDVLEKFGGTV 427
Query: 316 LPPGCGPCLG---RHMGVAGDNEVILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYG 371
L CGPC+G R G+ I+++ NRNF GR +P ++ SP A A+ G
Sbjct: 428 LANACGPCIGQWDRKDVKMGEKNTIVTSYNRNFTGRNDANPATHCFVTSPEMATALAIAG 487
Query: 372 EI 373
+
Sbjct: 488 RL 489
>sp|P49609|ACON_GRAVE ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSOR (CITRATE HYDRO-LYASE)
(ACONITASE) >gi|1361942|pir||S57805 aconitate hydratase
(EC 4.2.1.3) precursor - red alga (Gracilaria
verrucosa) >gi|600171|gb|AAA80494.1| (U17709) aconitase
[Gracilaria gracilis]
Length = 779
Score = 108 bits (268), Expect = 9e-23
Identities = 97/339 (28%), Positives = 154/339 (44%), Gaps = 51/339 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ+V+E A +++G DSHTP G LG AVG+G D A+ + +G W LK
Sbjct: 167 GSGIIHQIVLE-NYAFPGLLMIGTDSHTPNAGGLGACAVGVGGAD-AVDVMVGLPWELKA 224
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P+ I V L G +E+ DV++ + L + + +E+F S T+ N
Sbjct: 225 PKVIGVKLTGKLQEWASPKDVILKVAGILTVKGGTGAIVEYFGEGVDSLSCTGMGTICNM 284
Query: 200 SVEANAKTAI------IGEEYEGGG----------------------YSDEITIELDSLP 231
E A T++ +G+ + G Y I I L L
Sbjct: 285 GAEIGATTSMFPYNSRMGDYLKATGRDGIASLADSFSEQLRADENAVYDQLIEINLSELE 344
Query: 232 PMVAKPFSP--ANGVS--VEDVE----GTKIDQVFIGSCTNGRFEQIKRAAEILD---GE 280
P + PF+P A+ +S E+VE ++ IGSCTN +E + R+A ++
Sbjct: 345 PHINGPFTPDLAHPLSKFKEEVEKNGWPAELTVGLIGSCTNSSYEDMARSASVVKQALSH 404
Query: 281 QVNVRTL--VAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNE 335
V +++ + P S V + +G++ +AG +L CGPC+G R G
Sbjct: 405 GVKSKSIFNITPGSEQVRATISRDGILDTFTEAGGTVLANACGPCIGQWNRSDVPKGTPN 464
Query: 336 VILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEI 373
I+++ NRNF R G+P+ ++ASP +L G +
Sbjct: 465 SIITSFNRNFSQRNDGNPQTHAFVASPEIVTAMSLAGSL 503
>gb|AAC61778.1| (AF093142) aconitase [Aspergillus terreus]
Length = 778
Score = 107 bits (265), Expect = 2e-22
Identities = 112/414 (27%), Positives = 170/414 (41%), Gaps = 63/414 (15%)
Query: 21 DLVYAHDGTMPL-IIEAFNRIFNEVRTRAYIFFDHVYPAPT------VKVANLHREIREF 73
D V D T + I++ + V T + DH+ A + +++E+ +F
Sbjct: 91 DRVACQDATAQMAILQFMSAGMPSVATPTTVHCDHLIEAQVGGEKDLARANEINKEVYDF 150
Query: 74 -----AKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATD 128
AK + G GI HQ+V+E A +++G DSHTP G L + A+G+G D
Sbjct: 151 LATATAKYNIGFWKPGSGIIHQIVLE-NYAFPGGLMIGTDSHTPNAGGLAMAAIGVGGAD 209
Query: 129 VAIALGLGKIW-LKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV- 186
A+ + G W LK P+ I V L G + D+++ + L + + IE+
Sbjct: 210 -AVDVMAGLPWELKAPKVIGVKLTGEMSGWTTPKDIILKVAGLLTVKGGTGAIIEYHGPG 268
Query: 187 --PFSFDERLTLTNFSVEANAKTAI------------------IGE----------EYEG 216
S T+ N E A T++ IGE E EG
Sbjct: 269 VNSLSCTGMGTICNMGAEIGATTSMFPFNDRMYDYLKATKRQHIGEFARSYAKELREDEG 328
Query: 217 GGYSDEITIELDSLPPMVAKPFSPANGVSVE------DVEG--TKIDQVFIGSCTNGRFE 268
Y I I L L P + PF+P + D G ++ IGSCTN +E
Sbjct: 329 AEYDQLIEINLSELEPHINGPFTPDLATPISKFKEAVDANGWPEELKVGLIGSCTNSSYE 388
Query: 269 QIKRAAEIL-----DGEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPC 323
+ RAA I G + V P S + + +G ++ L + G VIL CGPC
Sbjct: 389 DMSRAASIARDALNHGVKSKSLFTVTPGSEQIRATIERDGQLQTLEEFGGVILANACGPC 448
Query: 324 LG---RHMGVAGDNEVILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEI 373
+G R G+ I+S+ NRNF GR +P ++ASP + G +
Sbjct: 449 IGQWDRKDVKKGEPNSIISSYNRNFTGRNDANPATHAFVASPDLVVAMCVAGTL 502
>gb|AAF01428.1|AF186471_1 (AF186471) aconitase [Xenopus laevis]
Length = 856
Score = 107 bits (264), Expect = 3e-22
Identities = 96/340 (28%), Positives = 150/340 (43%), Gaps = 52/340 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIW-LKV 142
G GI HQ+++E A +++G DSHTP G LG +G+G D A+ + G W LK
Sbjct: 171 GSGIIHQIILE-NYAFPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKC 228
Query: 143 PESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNF 199
P I V L G + DV++ + L + + +E+ S T+ N
Sbjct: 229 PNVIGVKLTGQLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNM 288
Query: 200 SVEANAKTAI---------------------IGEEYEGGGYSDE--------ITIELDSL 230
E A T++ + +E++ DE I I LD L
Sbjct: 289 GAEIGATTSVFPYNHRMKKYLDKTGRSEIASLTDEFKSNLVPDEGLLKYDQLIEINLDEL 348
Query: 231 PPMVAKPFSP--ANGV----SVEDVEGTKID--QVFIGSCTNGRFEQIKRAAEI----LD 278
P + PF+P AN V +V + +G +D IGSCTN +E + RAA + LD
Sbjct: 349 KPHINGPFTPDLANPVWEVGAVAEQKGWPLDIRVGLIGSCTNSSYEDMGRAAAVARQALD 408
Query: 279 -GEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDN 334
G + + + P S + + +G +L D G V+L CGPC+G R G+
Sbjct: 409 HGLKCKSQFTITPGSEQIRATIERDGYAAVLRDVGGVVLANACGPCIGQWDRKDIKKGEK 468
Query: 335 EVILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEI 373
I+++ NRNF GR +P+ ++ SP ++ G +
Sbjct: 469 NTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALSIAGTL 508
>sp|O13966|ACON_SCHPO ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSOR (CITRATE HYDRO-LYASE)
(ACONITASE) >gi|7490068|pir||T38347 aconitate hydratase
- fission yeast (Schizosaccharomyces pombe)
>gi|2330789|emb|CAB11263.1| (Z98601) aconitate hydratase
[Schizosaccharomyces pombe]
Length = 778
Score = 106 bits (263), Expect = 3e-22
Identities = 91/333 (27%), Positives = 141/333 (42%), Gaps = 51/333 (15%)
Query: 84 GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVP 143
G GI HQ+V+E A +++G DSHTP G LG+ A+G+G D + LK P
Sbjct: 165 GSGIIHQIVLE-NYAFPGGLLIGTDSHTPNAGGLGMVAIGVGGADAVDVMANLPWELKCP 223
Query: 144 ESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFN---VPFSFDERLTLTNFS 200
+ I V L G + + DV++ + L + + +E+F S T+ N
Sbjct: 224 KVIGVKLTGQLKGWTSPKDVILKVAGILTVKGGTGAIVEYFGPGVESLSCTGMGTICNMG 283
Query: 201 VEANAKTAII---------------------GEEY-------EGGGYSDEITIELDSLPP 232
E A T+I EE+ E Y I I+L++L P
Sbjct: 284 AEIGATTSIFPFNPRMSEYLRATNRSAIADYAEEFAPIIAADENAHYDQIIEIDLNTLEP 343
Query: 233 MVAKPFSPANGVSVEDVEGT--------KIDQVFIGSCTNGRFEQIKRAAEILD-----G 279
+ PF+P + + ++ IGSCTN +E + RAA I G
Sbjct: 344 HLNGPFTPDLATPISKFKEAVKKNDWPQELKVGLIGSCTNSSYEDMSRAASICQQAIDKG 403
Query: 280 EQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGV---AGDNEV 336
+ + P S V + +G + + AG ++L CGPC+G+ G+
Sbjct: 404 IKTKSLFTITPGSEQVRATLTRDGQLDTMRKAGGIVLANACGPCIGQWKRTDVKKGEKNS 463
Query: 337 ILSTTNRNFRGRM-GSPKAEIYLASP--VTAAV 366
I+++ NRNF GR +P ++ SP VTA V
Sbjct: 464 IVTSYNRNFTGRNDANPATHAFVTSPDIVTAMV 496
>emb|CAB72317.1| (AJ245736) aconitase [Daphnia pulex]
Length = 788
Score = 104 bits (256), Expect = 2e-21
Identities = 104/423 (24%), Positives = 167/423 (38%), Gaps = 63/423 (14%)
Query: 13 GEAVIR-KVDLVYAHDGTMPL-IIEAFNRIFNEVRTRAYIFFDHVYPAPT------VKVA 64
G++ +R + D V D T + +++ + V + I DH+ A +
Sbjct: 90 GQSYLRLRPDRVAMQDATAQMAMLQFISSGLPRVAVPSTIHCDHLIEAQVGGAQDLARAV 149
Query: 65 NLHREIREFAKIHGIPV-----VEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGV 119
++++E+ F G G GI HQ+++E A +++G DSHTP G LG
Sbjct: 150 DINKEVYNFLSTSGAKYGVGFWKPGSGIIHQIILE-NYAFPGLLMIGTDSHTPNGGGLGG 208
Query: 120 FAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYN 179
+G+G D + LK P+ I V L G + + DV++ + L + +
Sbjct: 209 LCIGVGGADAVDVMANIPWELKCPKVIGVKLTGKLQGWTSPKDVILKVAGILTVKGGTGA 268
Query: 180 AIEFFNV---PFSFDERLTLTNFSVEANAKTAII-------------------------G 211
+E+F S T+ N E A T++ G
Sbjct: 269 IVEYFGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMRDYLVATERKAIADEIDPYG 328
Query: 212 EEY----EGGGYSDEITIELDSLPPMVAKPFSP--ANGVSVEDVEGTK------IDQVFI 259
E+ E Y I + L L P V PF+P AN +S E K + I
Sbjct: 329 NEFLRADEDAPYDQVIELNLSELEPHVNGPFTPDLANPISKLGEEAKKNNWPLDVKVSLI 388
Query: 260 GSCTNGRFEQIKRAAEIL-----DGEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVV 314
GSCTN +E + R A I G + + P S + + +G+ L G +
Sbjct: 389 GSCTNSSYEDMGRCASIAREALKHGLKAKSAFNITPGSEQIRATIERDGIAETLRQFGGI 448
Query: 315 ILPPGCGPCLG---RHMGVAGDNEVILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALY 370
+L CGPC+G R G+ I+++ NRNF R +P ++ SP AL
Sbjct: 449 VLAQPCGPCIGQWDRKDVKKGEKNTIVTSYNRNFTARNDANPATHAFVTSPELTTALALA 508
Query: 371 GEI 373
G +
Sbjct: 509 GTL 511
>pir||S10171 3-isopropylmalate dehydratase (EC 4.2.1.33) chain leuC - Salmonella
typhimurium >gi|47762|emb|CAA35840.1| (X51476) leuC gene
product (AA 1-464) [Salmonella typhimurium]
>gi|154158|gb|AAA27155.1| (M31047) isopropylmalate
synthase (EC 4.2.1.3) [Salmonella typhimurium]
Length = 464
Score = 102 bits (253), Expect = 5e-21
Identities = 90/357 (25%), Positives = 139/357 (38%), Gaps = 67/357 (18%)
Query: 85 KGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPE 144
+GI H + E G+ IV GDSHT T GA G A G+G ++V L + +
Sbjct: 104 QGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAK 163
Query: 145 SIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNVPF---SFDERLTLTNFSV 201
++ + + G+ I A D+++ +I + + +EF S + R+TL N ++
Sbjct: 164 TMKIEVTGNAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAI 223
Query: 202 EANAKTAIIGE------------------------EY-------EGGGYSDEITIELDSL 230
E AK ++ EY +G + + + + +
Sbjct: 224 EMGAKAGLVAPDETTFNYVKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVALRAEEI 283
Query: 231 PPMVAKPFSPANGVSVEDV-------------------------------EGTKIDQVFI 259
P V +P +SV D+ ID+VFI
Sbjct: 284 APQVTWGTNPGQVISVTDIIPDPASFSDPVERASAEKALAYMGLQPGVPLTDVAIDKVFI 343
Query: 260 GSCTNGRFEQIKRAAEILDGEQVNVRTLVAPASINVYRRMIE-EGLVRILIDAGVVILPP 318
G CTN R E ++ AE+ +V R A E EGL +I I+AG P
Sbjct: 344 GPCTNSRIEDLRAPAEVGQRAKVAGRAGAGGAGFRSGESAAEAEGLDKIFIEAGFEWRLP 403
Query: 319 GCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITN 375
GC C+ + E ST+NRNF GR G +L SP AA +A+ G +
Sbjct: 404 GCSMCVAVNNDRLNPGERCASTSNRNFEGRQGR-GGRTHLVSPAMAAAAAVTGHFAD 459
>emb|CAB93521.1| (AJ252018) mitochondrial aconitase [Drosophila melanogaster]
>gi|10728908|gb|AAF53974.2| (AE003669) Acon gene product
[alt 2] [Drosophila melanogaster]
Length = 787
Score = 99.5 bits (244), Expect = 6e-20
Identities = 101/414 (24%), Positives = 168/414 (40%), Gaps = 62/414 (14%)
Query: 21 DLVYAHDGTMPL-IIEAFNRIFNEVRTRAYIFFDHVY------PAPTVKVANLHREIREF 73
D V D T + +++ + +V + + DH+ P + +L++E+ +F
Sbjct: 101 DRVAMQDATAQMALLQFISSGLKKVAVPSTVHCDHLIEAQIGGPKDLARAKDLNKEVYDF 160
Query: 74 AKI----HGIPVVE-GKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATD 128
+G+ + G GI HQ+++E A +++G DSHTP G LG +G+G D
Sbjct: 161 LASTCAKYGLGFWKPGSGIIHQIILE-NYAFPGLLMIGTDSHTPNGGGLGGLCIGVGGAD 219
Query: 129 VAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNV-- 186
+ LK P+ I V L G + DV++ + L + + IE+
Sbjct: 220 AVDVMADIPWELKCPKVIGVNLTGKISGWTSPKDVILKVADILTVKGGTGAIIEYHGKGV 279
Query: 187 -PFSFDERLTLTNFSVEANAKTAII---------------------GEEYEGG------- 217
S T+ N E A T++ ++Y+
Sbjct: 280 DSISCTGMATICNMGAEIGATTSLFPFNQRMADYLKSTGRAGIASEAQKYQAKILSADKN 339
Query: 218 -GYSDEITIELDSLPPMVAKPFSPANGVSVEDV-EGTK-------IDQVFIGSCTNGRFE 268
Y + I I LD+L P V PF+P G + + E +K I IGSCTN +E
Sbjct: 340 CEYDELIEINLDTLEPHVNGPFTPDLGHPISKLGENSKKNGYPMDIRVGLIGSCTNSSYE 399
Query: 269 QIKRAAEIL-----DGEQVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPC 323
+ R A I G + + V P S + + +G+ + G +L CGPC
Sbjct: 400 DMGRCASIAKDAMSHGLKSKIPFNVTPGSEQIRATIERDGISEVFDKFGGTVLANACGPC 459
Query: 324 LG---RHMGVAGDNEVILSTTNRNFRGRM-GSPKAEIYLASPVTAAVSALYGEI 373
+G R GD I+++ NRNF GR +P ++ SP ++ G +
Sbjct: 460 IGQWDRKDVKKGDKNTIVTSYNRNFTGRNDANPATHCFVTSPELVTALSIAGRL 513
>gb|AAF53973.1| (AE003669) Acon gene product [alt 1] [Drosophila melanogaster]
Length = 683
Score = 98.7 bits (242), Expect = 1e-19
Identities = 94/371 (25%), Positives = 152/371 (40%), Gaps = 55/371 (14%)
Query: 57 PAPTVKVANLHREIREFAKI----HGIPVVE-GKGISHQLVVELGLAEEAKIIVGGDSHT 111
P + +L++E+ +F +G+ + G GI HQ+++E A +++G DSHT
Sbjct: 40 PKDLARAKDLNKEVYDFLASTCAKYGLGFWKPGSGIIHQIILE-NYAFPGLLMIGTDSHT 98
Query: 112 PTLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSL 171
P G LG +G+G D + LK P+ I V L G + DV++ + L
Sbjct: 99 PNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVNLTGKISGWTSPKDVILKVADIL 158
Query: 172 KDREMSYNAIEFFNV---PFSFDERLTLTNFSVEANAKTAII------------------ 210
+ + IE+ S T+ N E A T++
Sbjct: 159 TVKGGTGAIIEYHGKGVDSISCTGMATICNMGAEIGATTSLFPFNQRMADYLKSTGRAGI 218
Query: 211 ---GEEYEGG--------GYSDEITIELDSLPPMVAKPFSPANGVSVEDV-EGTK----- 253
++Y+ Y + I I LD+L P V PF+P G + + E +K
Sbjct: 219 ASEAQKYQAKILSADKNCEYDELIEINLDTLEPHVNGPFTPDLGHPISKLGENSKKNGYP 278
Query: 254 --IDQVFIGSCTNGRFEQIKRAAEIL-----DGEQVNVRTLVAPASINVYRRMIEEGLVR 306
I IGSCTN +E + R A I G + + V P S + + +G+
Sbjct: 279 MDIRVGLIGSCTNSSYEDMGRCASIAKDAMSHGLKSKIPFNVTPGSEQIRATIERDGISE 338
Query: 307 ILIDAGVVILPPGCGPCLG---RHMGVAGDNEVILSTTNRNFRGRM-GSPKAEIYLASPV 362
+ G +L CGPC+G R GD I+++ NRNF GR +P ++ SP
Sbjct: 339 VFDKFGGTVLANACGPCIGQWDRKDVKKGDKNTIVTSYNRNFTGRNDANPATHCFVTSPE 398
Query: 363 TAAVSALYGEI 373
++ G +
Sbjct: 399 LVTALSIAGRL 409
>emb|CAA89720.1| (Z49701) unknown [Saccharomyces cerevisiae]
Length = 375
Score = 97.5 bits (239), Expect = 2e-19
Identities = 57/143 (39%), Positives = 83/143 (57%), Gaps = 11/143 (7%)
Query: 245 SVEDV--EGTKIDQVFIGSCTNGRFEQIKRAAEIL----DGEQVN-----VRTLVAPASI 293
+V+D+ + KI++ ++ SCTN R ++ AA+++ D +VN V VA AS
Sbjct: 3 TVQDLSQQDIKINKAYLVSCTNSRLSDLQSAADVVCPTGDLNKVNKVAPGVEFYVAAASS 62
Query: 294 NVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPK 353
+ + G L+ AG + LP GCGPC+G G+ EV +S TNRNF+GRMGS
Sbjct: 63 EIEADARKSGAWEKLLKAGCIPLPSGCGPCIGLGAGLLEPGEVGISATNRNFKGRMGSKD 122
Query: 354 AEIYLASPVTAAVSALYGEITNP 376
A YLASP A SA+ G+I++P
Sbjct: 123 ALAYLASPAVVAASAVLGKISSP 145
>pir||S45196 3-isopropylmalate dehydrogenase homolog - Escherichia coli
>gi|473786|dbj|BAA05575.1| (D26562) 'ORF' [Escherichia
coli]
Length = 773
Score = 97.5 bits (239), Expect = 2e-19
Identities = 106/393 (26%), Positives = 156/393 (38%), Gaps = 77/393 (19%)
Query: 52 FDHVYPAPTVKVANLHREIREFAKIHG-IPVVEGKGISHQLVVELGLAEEAKIIVGGDSH 110
F H P N H + +F G + + G G+ H + + L + + GGDSH
Sbjct: 350 FCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDT--VGTGGDSH 407
Query: 111 TPTLGALGV-FAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLIT 169
T +G+ F G G VA A G + L +PES+ V G + I D ++H I
Sbjct: 408 TRF--PIGISFPAGSGL--VAFAAATGVMPLDMPESVLVRFKGKMQPGITLRD-LVHAIP 462
Query: 170 SL-------------KDREMSYNAIEFFNVP-FSFDERLTLTNFSVEANAKTAIIGEEYE 215
K S +E +P ++ LT+ S E +A I E
Sbjct: 463 LYAIKQGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKE 522
Query: 216 -----------------GGGYSDEITIE-------------------------------- 226
GY D T+E
Sbjct: 523 PIIEYLNSNIVLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDL 582
Query: 227 LDSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEILDGE--QVNV 284
D P++ P P + + V+G KID+VFIGSC + A ++LD Q+
Sbjct: 583 ADIKEPILCAPNDPDDARPLSAVQGEKIDEVFIGSCMT-NIGHFRAAGKLLDAHKGQLPT 641
Query: 285 RTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRN 344
R VAP + ++ EEG + +G I PGC C+G VA D ++ST+ RN
Sbjct: 642 RLWVAPPTRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVA-DGATVVSTSTRN 700
Query: 345 FRGRMGSPKAEIYLASPVTAAVSALYGEITNPE 377
F R+G+ A ++LAS AAV+AL G++ PE
Sbjct: 701 FPNRLGT-GANVFLASAELAAVAALIGKLPTPE 732
>sp|P36683|ACO2_ECOLI ACONITATE HYDRATASE 2 (CITRATE HYDRO-LYASE 2) (ACONITASE 2)
>gi|7465770|pir||F64734 aconitate hydratase (EC 4.2.1.3)
2 - Escherichia coli >gi|2367097|gb|AAC73229.1|
(AE000121) aconitate hydrase B [Escherichia coli K12]
Length = 865
Score = 97.5 bits (239), Expect = 2e-19
Identities = 106/393 (26%), Positives = 156/393 (38%), Gaps = 77/393 (19%)
Query: 52 FDHVYPAPTVKVANLHREIREFAKIHG-IPVVEGKGISHQLVVELGLAEEAKIIVGGDSH 110
F H P N H + +F G + + G G+ H + + L + + GGDSH
Sbjct: 442 FCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDT--VGTGGDSH 499
Query: 111 TPTLGALGV-FAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLIT 169
T +G+ F G G VA A G + L +PES+ V G + I D ++H I
Sbjct: 500 TRF--PIGISFPAGSGL--VAFAAATGVMPLDMPESVLVRFKGKMQPGITLRD-LVHAIP 554
Query: 170 SL-------------KDREMSYNAIEFFNVP-FSFDERLTLTNFSVEANAKTAIIGEEYE 215
K S +E +P ++ LT+ S E +A I E
Sbjct: 555 LYAIKQGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKE 614
Query: 216 -----------------GGGYSDEITIE-------------------------------- 226
GY D T+E
Sbjct: 615 PIIEYLNSNIVLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDL 674
Query: 227 LDSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEILDGE--QVNV 284
D P++ P P + + V+G KID+VFIGSC + A ++LD Q+
Sbjct: 675 ADIKEPILCAPNDPDDARPLSAVQGEKIDEVFIGSCMT-NIGHFRAAGKLLDAHKGQLPT 733
Query: 285 RTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRN 344
R VAP + ++ EEG + +G I PGC C+G VA D ++ST+ RN
Sbjct: 734 RLWVAPPTRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVA-DGATVVSTSTRN 792
Query: 345 FRGRMGSPKAEIYLASPVTAAVSALYGEITNPE 377
F R+G+ A ++LAS AAV+AL G++ PE
Sbjct: 793 FPNRLGT-GANVFLASAELAAVAALIGKLPTPE 824
>gb|AAC35557.1| (AF086790) aconitase precursor [Homo sapiens]
Length = 600
Score = 96.3 bits (236), Expect = 5e-19
Identities = 85/324 (26%), Positives = 139/324 (42%), Gaps = 49/324 (15%)
Query: 103 IIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASD 162
+++G DSHTP G LG ++G+G D + LK + IA+ L GS + D
Sbjct: 8 LLIGTDSHTPNGGGLGGISIGVGGRDRVDVMAGIPCELKCTKVIAMKLTGSLSGWTSPKD 67
Query: 163 VMIHLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNFSVEANAKTAI---------- 209
V++ + L + + +E+ S T+ N E A T++
Sbjct: 68 VILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKY 127
Query: 210 -----------IGEEYE-------GGGYSDEITIELDSLPPMVAKPFSP------ANGVS 245
+ EE++ G Y I I L+ L P + PF+P A +
Sbjct: 128 LSKTGRADIASLREEFKDHLVPDPGCQYDQVIEINLNELKPHINGPFTPDLAHPVAEVGT 187
Query: 246 VEDVEGTKID--QVFIGSCTNGRFEQIKRAAEILD-----GEQVNVRTLVAPASINVYRR 298
V + EG +D IGSCTN +E + R+A + G + + + P S +
Sbjct: 188 VAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRAT 247
Query: 299 MIEEGLVRILIDAGVVILPPGCGPCLG---RHMGVAGDNEVILSTTNRNFRGRMGS-PKA 354
+ +G +IL D G ++L CGPC+G R G+ ++++ NRNF GR + P+
Sbjct: 248 IERDGYAQILRDVGGIVLANACGPCIGQRDRKDIKKGEKNTMVTSYNRNFTGRNDAFPET 307
Query: 355 EIYLASPVTAAVSALYGEIT-NPE 377
++ SP A+ G + NPE
Sbjct: 308 HAFVTSPEIVTALAIAGTLKFNPE 331
>pir||C82303 aconitate hydratase 2 VC0604 [imported] - Vibrio cholerae (group
O1 strain N16961) >gi|9655036|gb|AAF93771.1| (AE004145)
aconitate hydratase 2 [Vibrio cholerae]
Length = 865
Score = 94.4 bits (231), Expect = 2e-18
Identities = 121/445 (27%), Positives = 176/445 (39%), Gaps = 86/445 (19%)
Query: 3 LVGKLLNA---KPGEAVIRKVDLVYAHDGTMPLIIEAFNRI----FNEVRTRAYIFFDHV 55
+VGK +PG K+ V + D T P+ + + F+
Sbjct: 388 MVGKACGVQGIRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTSA 447
Query: 56 YPAPTVKVANLHREIREFAKIHG-IPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTL 114
YP P H + +F G + + G G+ H + + L + + GGDSHT
Sbjct: 448 YPKPVD--VQTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDT--VGTGGDSHTRF- 502
Query: 115 GALGV-FAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSL-- 171
LG+ F G G VA A G + L +PES+ V G + I D ++H I
Sbjct: 503 -PLGISFPAGSGL--VAFAAATGVMPLDMPESVLVRFKGKMQPGITLRD-LVHAIPYYGI 558
Query: 172 -----------KDREMSYNAIEFFNVP-FSFDERLTLTNFSVEANAKTAIIG------EE 213
K E S +E V + ++ L++ S E +A + EE
Sbjct: 559 KQGLLTVEKAGKINEFSGRILEIEGVEHLTVEQAFELSDASAERSAAGCTVKLSQASIEE 618
Query: 214 YEGG-----------GYSDEITIE--------------------------------LDSL 230
Y GY D TIE +
Sbjct: 619 YLNSNIVMLKWMIAEGYGDRRTIERRIQAMEAWLANPTLMEADKDAQYAHVIEIDLAEIK 678
Query: 231 PPMVAKPFSPANGVSVEDVEGTKIDQVFIGSC-TN-GRFEQIKRAAEILDGEQVNVRTLV 288
P++ P P + + + G I++VFIGSC TN G F + E +G Q+N R V
Sbjct: 679 EPILCAPNDPDDARLLSECAGETINEVFIGSCMTNIGHFRAAGKLLEKFNG-QLNTRLWV 737
Query: 289 APASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGR 348
AP + ++IEEG I AGV I PGC C+G VA D ++ST+ RNF R
Sbjct: 738 APPTKMDRDQLIEEGYYGIFGRAGVRIETPGCSLCMGNQARVA-DKATVMSTSTRNFPNR 796
Query: 349 MGSPKAEIYLASPVTAAVSALYGEI 373
+G+ A +YL+S AAV A+ G I
Sbjct: 797 LGT-GANVYLSSAELAAVGAILGRI 820
>gb|AAK41354.1| Aconitate hydratase [Sulfolobus solfataricus]
Length = 855
Score = 94.4 bits (231), Expect = 2e-18
Identities = 92/371 (24%), Positives = 144/371 (38%), Gaps = 78/371 (21%)
Query: 81 VVEGKGISHQLVVELGLAEEAK------------IIVGGDSHTPTLGALGVFAVGMGATD 128
V GKGI HQ+ +E AK +I+G DSHT + LG+ G+G +
Sbjct: 161 VPPGKGIIHQVNLEYLSTVVAKAEVKGLLTAFPEVIIGTDSHTTMIEGLGILGWGVGGLE 220
Query: 129 VAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNVPF 188
L +L VPE I V L G +E + +DV++++ L+ + + +EFF
Sbjct: 221 AEAVLLGEPYYLNVPEVIGVRLTGEIQEGVTPTDVVLYITELLRKKNVVSKFVEFFGPSL 280
Query: 189 SF---DERLTLTNFSVEANAKTA------------------------------IIGEEYE 215
S +R T+ N + E A A + ++
Sbjct: 281 SLLSVPDRATIANMAPEYGATAAYFPIDDVTVSYLELTNRDGEFVKKYAQLQELFYDDSR 340
Query: 216 GGGYSDEITIELDSLPPMVAKPFSPANGVSVEDVEG---------------TKIDQVFIG 260
YSD + ++L + P +A P +P +S+ +V+G + I
Sbjct: 341 KIRYSDIVEVDLSKIEPAIAGPRNPDERISLREVKGKLSKEKKKKGKYVEDNAVVLAAIT 400
Query: 261 SCTNGRFEQIKRAAEILDGEQVN--------VRTLVAPASINVYRRMIEEGLVRILIDAG 312
SCTN + A IL + V ++T AP S V + E GL+ L G
Sbjct: 401 SCTNTSNPTVMLGAGILAKKAVEMGLRVPPYIKTSTAPGSPVVAEYLKETGLLPYLEALG 460
Query: 313 VVILPPGCGPCLG----------RHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPV 362
++ GC C+G + G + + NRNF GR+ +LASP+
Sbjct: 461 FHLVGFGCTTCIGNAGPLPKYVEEDIKENGIETYAVISGNRNFEGRINPLLKGTFLASPI 520
Query: 363 TAAVSALYGEI 373
AL G I
Sbjct: 521 LVVAYALAGRI 531
>sp|Q44023|LEU2_ALCEU 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|2120952|pir||I39573 probable 3-isopropylmalate
dehydratase (EC 4.2.1.33) large chain [similarity] -
Alcaligenes eutrophus (fragment)
>gi|695283|gb|AAC41429.1| (L36817) ORF11 [Ralstonia
eutropha] >gi|1093470|prf||2104199L ORF 11 [Ralstonia
eutropha]
Length = 267
Score = 89.3 bits (218), Expect = 6e-17
Identities = 74/250 (29%), Positives = 102/250 (40%), Gaps = 65/250 (26%)
Query: 191 DERLTLTNFSVEANAKTAII-----------GEEYEGGG-------------YSD----- 221
+ RLTL N +VE +A + I+ G Y G YSD
Sbjct: 13 EARLTLCNLAVEFSAFSGIVAPDDTVFEYLAGRPYAPAGAQWEPALWHWRSLYSDADAVF 72
Query: 222 --EITIELDSLPPMVAKPFSPANGVSVED------------------------------- 248
E++++ L PMV SP +GV+V+
Sbjct: 73 DRELSVDCRQLAPMVTWGTSPQHGVAVDGAVPNPAMAIDADTRQAMERALSYMDLRPGQR 132
Query: 249 VEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVR 306
+ ID FIGSCTN R ++ AA +L G +V V + P S V R EGL R
Sbjct: 133 MADIAIDAAFIGSCTNSRLSDLRSAAGVLAGRKVAPGVTAICVPVSSAVKRAAEAEGLDR 192
Query: 307 ILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAV 366
+ +AG GC C G + ++S+TNRNF R G P+ +LA P T A
Sbjct: 193 VFREAGFEWRESGCSMCFYAGGESFGHRQRVISSTNRNFESRQG-PQTRTHLAGPATVAA 251
Query: 367 SALYGEITNP 376
SA+ G I +P
Sbjct: 252 SAVLGRIADP 261
>gb|AAD32176.1|AF125563_1 (AF125563) putative aconitate hydratase [Neisseria meningitidis]
Length = 861
Score = 88.9 bits (217), Expect = 8e-17
Identities = 97/391 (24%), Positives = 156/391 (39%), Gaps = 74/391 (18%)
Query: 52 FDHVYPAPTVKVANLHREIREFAKIHG-IPVVEGKGISHQLVVELGLAEEAKIIVGGDSH 110
F H P H+E+ F G + + G G+ H + L L + + GGDSH
Sbjct: 443 FCHTAAYPKPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDT--VGTGGDSH 500
Query: 111 TPTLGALGV-FAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDV------ 163
T +G+ F G G VA A G + L +PES+ V G + + D+
Sbjct: 501 TRF--PIGISFPAGSGL--VAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPL 556
Query: 164 ------MIHLITSLKDREMSYNAIEFFNVP-FSFDERLTLTNFSVEANA----------- 205
++ + + K S +E +P ++ LT+ S E +A
Sbjct: 557 YAIKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEP 616
Query: 206 ------KTAIIGEEYEGGGYSDEITIEL-------------------------------- 227
++ + GY D T+E
Sbjct: 617 IIEYMKSNVVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMD 676
Query: 228 DSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEILDGE-QVNVRT 286
D P++A P P + + + GTKID+VFIGSC + A+++L+G+ + VR
Sbjct: 677 DIKEPIIACPNDPDDVCFMSERSGTKIDEVFIGSCMT-NIGHFRAASKLLEGKSDIPVRL 735
Query: 287 LVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFR 346
VAP + + + +EG +L AG + PGC C+G V + ++ST+ RNF
Sbjct: 736 WVAPPTKMDAKELSDEGHYGVLGRAGARMEMPGCSLCMGNQAQV-HEGATVMSTSTRNFP 794
Query: 347 GRMGSPKAEIYLASPVTAAVSALYGEITNPE 377
R+G +YL S AA+ + G+I E
Sbjct: 795 NRLGK-NTFVYLGSAELAAICSKLGKIPTVE 824
>pir||G81068 aconitate hydratase 2 NMB1572 [imported] - Neisseria meningitidis
(group B strain MD58) >gi|7226819|gb|AAF41925.1|
(AE002507) aconitate hydratase 2 [Neisseria meningitidis
MC58]
Length = 861
Score = 88.2 bits (215), Expect = 1e-16
Identities = 96/391 (24%), Positives = 156/391 (39%), Gaps = 74/391 (18%)
Query: 52 FDHVYPAPTVKVANLHREIREFAKIHG-IPVVEGKGISHQLVVELGLAEEAKIIVGGDSH 110
F H P H+E+ F G + + G G+ H + L L + + GGDSH
Sbjct: 443 FCHTAAYPKPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDT--VGTGGDSH 500
Query: 111 TPTLGALGV-FAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDV------ 163
T +G+ F G G VA A G + L +PES+ V G + + D+
Sbjct: 501 TRF--PIGISFPAGSGL--VAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPL 556
Query: 164 ------MIHLITSLKDREMSYNAIEFFNVP-FSFDERLTLTNFSVEANA----------- 205
++ + + K S +E +P ++ LT+ S E +A
Sbjct: 557 YAIKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEP 616
Query: 206 ------KTAIIGEEYEGGGYSDEITIEL-------------------------------- 227
++ + GY D T+E
Sbjct: 617 IIEYMKSNVVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMD 676
Query: 228 DSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEILDGE-QVNVRT 286
D P++A P P + + + GTKID+VFIGSC + A+++L+G+ VR
Sbjct: 677 DIKEPIIACPNDPDDVCFMSERSGTKIDEVFIGSCMT-NIGHFRAASKLLEGKADTPVRL 735
Query: 287 LVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFR 346
+AP + +++ +EG +L AG + PGC C+G V + ++ST+ RNF
Sbjct: 736 WIAPPTKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVR-EGATVMSTSTRNFP 794
Query: 347 GRMGSPKAEIYLASPVTAAVSALYGEITNPE 377
R+G +YL S AA+ + G+I E
Sbjct: 795 NRLGK-NTFVYLGSAELAAICSKLGKIPTVE 824
>pir||A81801 aconitate hydratase (EC 4.2.1.3) NMA1761 [imported] - Neisseria
meningitidis (group A strain Z2491)
>gi|7380403|emb|CAB84989.1| (AL162757) aconitate
hydratase [Neisseria meningitidis Z2491]
Length = 861
Score = 88.2 bits (215), Expect = 1e-16
Identities = 96/391 (24%), Positives = 156/391 (39%), Gaps = 74/391 (18%)
Query: 52 FDHVYPAPTVKVANLHREIREFAKIHG-IPVVEGKGISHQLVVELGLAEEAKIIVGGDSH 110
F H P H+E+ F G + + G G+ H + L L + + GGDSH
Sbjct: 443 FCHTAAYPKPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDT--VGTGGDSH 500
Query: 111 TPTLGALGV-FAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDV------ 163
T +G+ F G G VA A G + L +PES+ V G + + D+
Sbjct: 501 TRF--PIGISFPAGSGL--VAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPL 556
Query: 164 ------MIHLITSLKDREMSYNAIEFFNVP-FSFDERLTLTNFSVEANA----------- 205
++ + + K S +E +P ++ LT+ S E +A
Sbjct: 557 YAIKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEP 616
Query: 206 ------KTAIIGEEYEGGGYSDEITIEL-------------------------------- 227
++ + GY D T+E
Sbjct: 617 IIEYMKSNVVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMD 676
Query: 228 DSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEILDGE-QVNVRT 286
D P++A P P + + + GTKID+VFIGSC + A+++L+G+ VR
Sbjct: 677 DIKEPIIACPNDPDDVCFMSERSGTKIDEVFIGSCMT-NIGHFRAASKLLEGKADTPVRL 735
Query: 287 LVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFR 346
+AP + +++ +EG +L AG + PGC C+G V + ++ST+ RNF
Sbjct: 736 WIAPPTKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVR-EGATVMSTSTRNFP 794
Query: 347 GRMGSPKAEIYLASPVTAAVSALYGEITNPE 377
R+G +YL S AA+ + G+I E
Sbjct: 795 NRLGK-NTFVYLGSAELAAICSKLGKIPTVE 824
>sp|P74582|ACO2_SYNY3 ACONITATE HYDRATASE 2 (CITRATE HYDRO-LYASE 2) (ACONITASE 2)
>gi|7469257|pir||S76777 aconitate hydratase (EC 4.2.1.3)
2 - Synechocystis sp. (strain PCC 6803)
>gi|1653778|dbj|BAA18689.1| (D90916) hypothetical
protein [Synechocystis sp.]
Length = 868
Score = 86.2 bits (210), Expect = 6e-16
Identities = 60/161 (37%), Positives = 82/161 (50%), Gaps = 5/161 (3%)
Query: 219 YSDEITIELDSLP-PMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEIL 277
Y+D I + LD + P+VA P P N + + G I +VFIGSC + AA+IL
Sbjct: 656 YADVIEVNLDEIKEPIVAAPNDPDNVKLMSECAGDVIHEVFIGSCMT-NIGHYRAAAKIL 714
Query: 278 DGE-QVNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEV 336
+G V R + P + +++ EEG+ I AG PGC C+G V D
Sbjct: 715 EGAGTVKGRLWICPPTRMDEQQLREEGIYGIFAAAGARTEMPGCSLCMGNQARVE-DGVT 773
Query: 337 ILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNPE 377
+ ST+ RNF RMG A++YL S AAV AL G+I E
Sbjct: 774 VFSTSTRNFNNRMGK-GAQVYLGSAELAAVCALLGKIPTVE 813
>emb|CAA72703.1| (Y11966) 3-isopropylmalate dehydratase subunit [Buchnera
aphidicola]
Length = 466
Score = 82.7 bits (201), Expect = 6e-15
Identities = 47/120 (39%), Positives = 69/120 (57%), Gaps = 3/120 (2%)
Query: 254 IDQVFIGSCTNGRFEQIKRAAEILDGEQV--NVRTLVAPASINVYRRMIEEGLVRILIDA 311
+D+VFIGSCTN R E ++ A++I+ ++V + +V P S V + +EGL +I I+A
Sbjct: 339 VDKVFIGSCTNSRIEDLRIASKIIKNKKVAKTTKAIVVPGSKLVKIQAEKEGLDKIFINA 398
Query: 312 GVVILPPGCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYG 371
G PGC CL + E ST+NRNF R G +L SP+TAA +A++G
Sbjct: 399 GFEWRLPGCSMCLAMNDDKLNRGERCASTSNRNFEDRQGR-GGRTHLVSPITAAAAAIFG 457
>pir||G83422 aconitate hydratase 2 PA1787 [imported] - Pseudomonas aeruginosa
(strain PAO1) >gi|9947768|gb|AAG05176.1|AE004604_6
(AE004604) aconitate hydratase 2 [Pseudomonas
aeruginosa]
Length = 869
Score = 81.1 bits (197), Expect = 2e-14
Identities = 102/394 (25%), Positives = 157/394 (38%), Gaps = 79/394 (20%)
Query: 52 FDHVYPAPTVKVANLHREIREFAKIHG-IPVVEGKGISHQLVVELGLAEEAKIIVGGDSH 110
F H P H + +F G + + G GI H + + L + + GGDSH
Sbjct: 445 FCHTAAYPKPIDVKTHHTLPDFIMTRGGVSLRPGDGIIHSWLNRMLLPDT--VGTGGDSH 502
Query: 111 TPTLGALGV-FAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIH--- 166
T +G+ F G G VA A G + L +PES+ V G + I D ++H
Sbjct: 503 TRF--PIGISFPAGSGL--VAFAAATGVMPLDMPESVLVRFKGKLQPGITLRD-LVHAIP 557
Query: 167 --------LITSLKDREMSYNA----IEFFNVPFSFDERLTLTNFSVEANAKTAIIG--- 211
L K ++ ++ IE N + ++ L++ S E +A I
Sbjct: 558 YYAIQQGLLTVEKKGKKNIFSGRILEIEGLN-DLTVEQAFELSDASAERSAAGCTIKLPE 616
Query: 212 ---EEYE-----------GGGYSDEITIE------------------------------- 226
EY G GY D T+E
Sbjct: 617 QAIAEYLKSNITLLRWMIGEGYGDPRTLERRAQAMEAWLAKPELLEADKDAEYAAVIEID 676
Query: 227 -LDSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEILDGEQ--VN 283
D P++ P P + + V+G KID+VFIGSC + A ++LD + +
Sbjct: 677 LADVKEPVLCAPNDPDDARLLSSVQGRKIDEVFIGSCMT-NIGHFRAAGKLLDKVKGGIP 735
Query: 284 VRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEVILSTTNR 343
R +AP + ++ EEG I AG + PGC C+G V + V+ ST+ R
Sbjct: 736 TRLWLAPPTKMDAHQLTEEGYYGIYGKAGARMEMPGCSLCMGNQARVQTGSTVV-STSTR 794
Query: 344 NFRGRMGSPKAEIYLASPVTAAVSALYGEITNPE 377
NF R+G +++LAS AAVS++ G++ E
Sbjct: 795 NFPNRLGD-ATDVFLASAELAAVSSILGKLPTVE 827
>emb|CAA88513.1| (Z48612) unknown [Saccharomyces cerevisiae]
Length = 324
Score = 77.6 bits (188), Expect = 2e-13
Identities = 56/211 (26%), Positives = 97/211 (45%), Gaps = 8/211 (3%)
Query: 5 GKLLNAKPGEAVIRKVDLVYAHDGTMPLIIEAFNRIFNEVRTRAYIF--FDHVYPAPTVK 62
GK++++ G+ V K +HD + P+ ++ +++ + I DH + K
Sbjct: 38 GKVVHS--GDYVSIKPAHCMSHDNSWPVALKFMGLGATKIKNPSQIVTTLDHDIQNKSEK 95
Query: 63 VANLHREIREFAKIHGIP-VVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFA 121
++ I FAK H I G+GI HQ+++E G A + V DSH+ T G LG
Sbjct: 96 NLTKYKNIENFAKKHHIDHYPAGRGIGHQIMIEEGYAFPLNMTVASDSHSNTYGGLGSLG 155
Query: 122 VGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAI 181
+ TD A G+ W ++P V L G + + D+++ L + ++ +AI
Sbjct: 156 TPIVRTDAAAIWATGQTWWQIPPVAQVELKGQLPQGVSGKDIIVALCGLFNNDQVLNHAI 215
Query: 182 EFFNVPFS---FDERLTLTNFSVEANAKTAI 209
EF + D RLT+ N + E A + +
Sbjct: 216 EFTGDSLNALPIDHRLTIANMTTEWGALSGL 246
>pir||A82825 aconitate hydratase 2 XF0292 [imported] - Xylella fastidiosa
(strain 9a5c) >gi|9105113|gb|AAF83105.1|AE003882_7
(AE003882) aconitate hydratase 2 [Xylella fastidiosa]
Length = 886
Score = 77.3 bits (187), Expect = 3e-13
Identities = 53/157 (33%), Positives = 83/157 (52%), Gaps = 5/157 (3%)
Query: 219 YSDEITIEL-DSLPPMVAKPFSPANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKRAAEIL 277
Y+ I I+L D P+VA P P + ++ +V G KID+VFIGSC + AA++L
Sbjct: 692 YAAVIEIDLADIHEPIVACPNDPDDVKTLSEVAGAKIDEVFIGSCMT-NIGHFRAAAKLL 750
Query: 278 DGEQ-VNVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVAGDNEV 336
+G++ + R VAP + + +EG +AG + PGC C+G + A +
Sbjct: 751 EGKRDIPTRLWVAPPTKMDASELTKEGYYGTFGNAGARMEMPGCSLCMG-NQAQAREGAT 809
Query: 337 ILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEI 373
+ ST+ RNF R+G +YL S AA+ + G+I
Sbjct: 810 VFSTSTRNFPNRLGR-NTNVYLGSAELAAICSRLGKI 845
>pir||C81356 aconitate hydratase (EC 4.2.1.3) Cj0835c [imported] - Campylobacter
jejuni (strain NCTC 11168) >gi|6968281|emb|CAB73100.1|
(AL139076) aconitate hydratase [Campylobacter jejuni]
Length = 848
Score = 72.2 bits (174), Expect = 9e-12
Identities = 56/166 (33%), Positives = 81/166 (48%), Gaps = 10/166 (6%)
Query: 219 YSDEITIELDSLP-PMVAKPFSP-----ANGVSVEDVEGTKIDQVFIGSCTNGRFEQIKR 272
Y+ I I L+++ P++A P P + + +D ID+VF+GSC +
Sbjct: 652 YAYIIDINLNNIKEPILACPNDPDDVATLSEILADDKRPKNIDEVFVGSCMTN-IGHYRA 710
Query: 273 AAEILDGEQV-NVRTLVAPASINVYRRMIEEGLVRILIDAGVVILPPGCGPCLGRHMGVA 331
EIL + + R V P + ++I EG I AG I PGC C+G V
Sbjct: 711 LGEILKDKGILKTRLWVVPPTKMDKAQLINEGYYSIFGAAGARIEVPGCSLCMGNQARV- 769
Query: 332 GDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNPE 377
D V+ ST+ RNF RMG A++YL S AAV A+ G+I + E
Sbjct: 770 NDGAVVFSTSTRNFDNRMGM-GAKVYLGSAELAAVCAILGKIPSKE 814
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.319 0.139 0.398
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 141705179
Number of Sequences: 2977
Number of extensions: 6095143
Number of successful extensions: 14316
Number of sequences better than 1.0e-10: 119
Number of HSP's better than 0.0 without gapping: 25
Number of HSP's successfully gapped in prelim test: 94
Number of HSP's that attempted gapping in prelim test: 13957
Number of HSP's gapped (non-prelim): 194
length of query: 380
length of database: 189,106,746
effective HSP length: 56
effective length of query: 324
effective length of database: 155,591,474
effective search space: 50411637576
effective search space used: 50411637576
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 166 (69.1 bits)