BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0288 (leuD-2) DE:3-isopropylmalate dehydratase, small
subunit (leuD-2)
(163 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||A75160 3-isopropylmalate dehydratase, small chain (leud-2) ... 331 3e-90
sp|O59393|LEUD_PYRHO PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE SMAL... 272 1e-72
gi|11499350 3-isopropylmalate dehydratase, small subunit (leuD-2... 168 3e-41
pir||C75045 3-isopropylmalate dehydratase, small chain (leud-1) ... 166 2e-40
pir||D72394 hypothetical protein TM0292 - Thermotoga maritima (s... 163 9e-40
sp|Q58673|LE24_METJA PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE SMAL... 162 2e-39
sp|O27440|LE24_METTH PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE SMAL... 161 4e-39
gi|11498237 3-isopropylmalate dehydratase, small subunit (leuD-1... 161 6e-39
sp|O26917|LE23_METTH PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE SMAL... 148 3e-35
sp|Q9WZ25|LEUD_THEMA 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT... 148 3e-35
sp|Q58667|LE23_METJA PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE SMAL... 144 5e-34
pir||H75373 3-isopropylmalate dehydratase, small subunit - Deino... 143 2e-33
pir||E70421 3-isopropylmalate dehydratase - Aquifex aeolicus >gi... 142 2e-33
sp|O67399|LEUD_AQUAE 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT... 142 2e-33
gb|AAK42607.1| 3-isopropylmalate dehydratase, small subunit (iso... 140 8e-33
dbj|BAA74763.1| (AB017109) HacB [Thermus thermophilus] 136 1e-31
pir||F75355 3-isopropylmalate dehydratase, small subunit - Deino... 116 1e-25
sp|Q92412|LYS4_EMENI HOMOACONITASE PRECURSOR (HOMOACONITATE HYDR... 89 3e-17
emb|CAA89720.1| (Z49701) unknown [Saccharomyces cerevisiae] 84 8e-16
gi|6320440 homoaconitase; Lys4p [Saccharomyces cerevisiae] >gi|1... 84 8e-16
gb|AAC64298.1| (AC004450) 3-isopropylmalate dehydratase, small s... 78 6e-14
pir||T47781 3-isopropylmalate dehydratase-like protein (small su... 77 1e-13
pir||T38665 probable homoaconitate hydratase (EC 4.2.1.36) - fis... 76 2e-13
pir||F70453 aconitase - Aquifex aeolicus >gi|2984078|gb|AAC07617... 72 4e-12
gb|AAC64299.1| (AC004450) 3-isopropylmalate dehydratase, small s... 69 2e-11
gb|AAG20620.1| (AE005132) aconitase; Can [Halobacterium sp. NRC-1] 68 8e-11
>pir||A75160 3-isopropylmalate dehydratase, small chain (leud-2) PAB0288 -
Pyrococcus abyssi (strain Orsay)
>gi|5457870|emb|CAB49360.1| (AJ248284) 3-isopropylmalate
dehydratase, small subunit (leuD-2) [Pyrococcus abyssi]
Length = 163
Score = 331 bits (840), Expect = 3e-90
Identities = 163/163 (100%), Positives = 163/163 (100%)
Query: 1 MITTGRVWKFWDNVSTDEITPGRYNLTKDPQELARIAFIEVRPEFAEKVRRGDVVVGGKN 60
MITTGRVWKFWDNVSTDEITPGRYNLTKDPQELARIAFIEVRPEFAEKVRRGDVVVGGKN
Sbjct: 1 MITTGRVWKFWDNVSTDEITPGRYNLTKDPQELARIAFIEVRPEFAEKVRRGDVVVGGKN 60
Query: 61 FGIGSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLLIGDTDELEDGDVITVNW 120
FGIGSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLLIGDTDELEDGDVITVNW
Sbjct: 61 FGIGSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLLIGDTDELEDGDVITVNW 120
Query: 121 ETGEVRKNGQTLQFEPLPGFLLEIVREGGILEFIRRRGDLCIG 163
ETGEVRKNGQTLQFEPLPGFLLEIVREGGILEFIRRRGDLCIG
Sbjct: 121 ETGEVRKNGQTLQFEPLPGFLLEIVREGGILEFIRRRGDLCIG 163
>sp|O59393|LEUD_PYRHO PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7520855|pir||G71180 probable 3-isopropylmalate
dehydratase - Pyrococcus horikoshii
>gi|3258155|dbj|BAA30838.1| (AP000007) 163aa long
hypothetical 3-isopropylmalate dehydratase [Pyrococcus
horikoshii]
Length = 163
Score = 272 bits (689), Expect = 1e-72
Identities = 129/162 (79%), Positives = 148/162 (90%)
Query: 1 MITTGRVWKFWDNVSTDEITPGRYNLTKDPQELARIAFIEVRPEFAEKVRRGDVVVGGKN 60
MITTG+VWKF D++STDEITPGRYNLTKDP+ELA+IAFIEVRP+FA VR GDVVV GKN
Sbjct: 1 MITTGKVWKFGDDISTDEITPGRYNLTKDPKELAKIAFIEVRPDFARNVRPGDVVVAGKN 60
Query: 61 FGIGSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLLIGDTDELEDGDVITVNW 120
FGIGSSRESAALALKA G++G+IA+SFGRIFYRNA+N+GIPLL+G T+ L+DGD++TVNW
Sbjct: 61 FGIGSSRESAALALKALGIAGVIAESFGRIFYRNAINIGIPLLLGKTEGLKDGDLVTVNW 120
Query: 121 ETGEVRKNGQTLQFEPLPGFLLEIVREGGILEFIRRRGDLCI 162
ETGEVRK + L FEPL FLLEIVREGGILE+IRRRGDLCI
Sbjct: 121 ETGEVRKGDEILMFEPLEDFLLEIVREGGILEYIRRRGDLCI 162
>gi|11499350 3-isopropylmalate dehydratase, small subunit (leuD-2)
[Archaeoglobus fulgidus]
>gi|3122335|sp|O28513|LE24_ARCFU PUTATIVE
3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7482881|pir||H69469 3-isopropylmalate dehydratase,
small subunit (leuD-2) homolog - Archaeoglobus fulgidus
>gi|2648789|gb|AAB89489.1| (AE000981) 3-isopropylmalate
dehydratase, small subunit (leuD-2) [Archaeoglobus
fulgidus]
Length = 165
Score = 168 bits (422), Expect = 3e-41
Identities = 83/165 (50%), Positives = 116/165 (70%), Gaps = 3/165 (1%)
Query: 1 MITTGRVWKFWDNVSTDEITPGRY-NLTKDPQELARIAFIEVRPEFAEKVRRGDVVVGGK 59
M GR WKF D++STD ITPGRY +L + ELA+ + P+F +K R GD +V GK
Sbjct: 1 MKMVGRAWKFGDDISTDHITPGRYYHLRSNMPELAKHVMEDADPDFMKKFRPGDFIVAGK 60
Query: 60 NFGIGSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLLIGDTDELEDGDVITVN 119
NFG+GSSRE A LALK AGVS +IAKSF RIFYRNA+N+G+P+LI DTD ++ GD + V+
Sbjct: 61 NFGMGSSREHAPLALKIAGVSAVIAKSFARIFYRNAINVGLPVLIADTDSIDSGDELEVD 120
Query: 120 WETGEV--RKNGQTLQFEPLPGFLLEIVREGGILEFIRRRGDLCI 162
G V R G ++ +P+P +L+I+ EGG++ ++++ GD+ I
Sbjct: 121 LGKGLVVNRTKGIEIRVKPIPEVMLKILHEGGLVSYVKKHGDIKI 165
>pir||C75045 3-isopropylmalate dehydratase, small chain (leud-1) PAB0892 -
Pyrococcus abyssi (strain Orsay)
>gi|5458769|emb|CAB50256.1| (AJ248287) 3-isopropylmalate
dehydratase, small subunit (leuD-1) [Pyrococcus abyssi]
Length = 164
Score = 166 bits (415), Expect = 2e-40
Identities = 79/161 (49%), Positives = 112/161 (69%), Gaps = 4/161 (2%)
Query: 1 MITTGRVWKFWDNVSTDEITPGRYNLTKDPQELARIAFIEVRPEFAEKVRRGDVVVGGKN 60
M GR WK+ DN+ TD I P RY T DP+ELA+ ++ PEF K++ GD++V G+N
Sbjct: 1 MRVRGRAWKYGDNIDTDVIIPARYLNTSDPKELAKHVLEDLDPEFRSKMKPGDIIVAGEN 60
Query: 61 FGIGSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLLIGD--TDELEDGDVITV 118
FG GSSRE A LA+KAAGVS +IAKSF RIFYRNA+N+G+P+L D +E GD + V
Sbjct: 61 FGCGSSREHAPLAIKAAGVSCVIAKSFARIFYRNAINIGLPILEAPQAVDRIETGDELEV 120
Query: 119 NWETGEVRK--NGQTLQFEPLPGFLLEIVREGGILEFIRRR 157
++ +GE+R G+ + P P F++EI++ GG++E+ +RR
Sbjct: 121 DFSSGEIRNLTKGEVYRANPFPDFIMEIIKAGGLVEWAKRR 161
>pir||D72394 hypothetical protein TM0292 - Thermotoga maritima (strain MSB8)
>gi|4980791|gb|AAD35380.1|AE001711_3 (AE001711)
3-isopropylmalate dehydratase, small subunit, putative
[Thermotoga maritima]
Length = 166
Score = 163 bits (409), Expect = 9e-40
Identities = 78/158 (49%), Positives = 116/158 (73%), Gaps = 4/158 (2%)
Query: 5 GRVWKFWDNVSTDEITPGRY-NLTKDPQELARIAFIEVRPEFAEKVRRGDVVVGGKNFGI 63
GRVWKF DN+STD I PGRY +L + +ELA+ + +FA+KV++GD++V GKNFG+
Sbjct: 4 GRVWKFGDNISTDHIAPGRYFHLRNNLEELAKHVLEDAMEDFAKKVQKGDIIVAGKNFGL 63
Query: 64 GSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLL-IGDTDELEDGDVITVNWET 122
GSSRE AA +K AGVS I+AKSF RIFYRNA+N+G+P++ + + DE+ GD + ++ E
Sbjct: 64 GSSREHAARIIKIAGVSCIVAKSFARIFYRNAINVGLPVIELKEVDEINQGDELEIDLEN 123
Query: 123 GEVRK--NGQTLQFEPLPGFLLEIVREGGILEFIRRRG 158
G ++ G+ +F P+P FLLEI++E GI+ ++++ G
Sbjct: 124 GVLKNLTTGKEYRFTPIPKFLLEILKEDGIVNYLKKHG 161
>sp|Q58673|LE24_METJA PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|2127721|pir||D64459 3-isopropylmalate dehydratase
(EC 4.2.1.33) - Methanococcus jannaschii
>gi|1591913|gb|AAB99283.1| (U67568) 3-isopropylmalate
dehydratase (leuD) [Methanococcus jannaschii]
Length = 168
Score = 162 bits (406), Expect = 2e-39
Identities = 77/160 (48%), Positives = 114/160 (71%), Gaps = 4/160 (2%)
Query: 2 ITTGRVWKFWDNVSTDEITPGRYNLTKDPQELARIAFIEVRPEFAEKVRRGDVVVGGKNF 61
I GRVWKF +NV TD I P RY + P+ELA+ P+F +KV+ GD++VGGKNF
Sbjct: 4 IIKGRVWKFGNNVDTDAILPARYLVYTKPEELAQFVMTGADPDFPKKVKPGDIIVGGKNF 63
Query: 62 GIGSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLL--IGDTDELEDGDVITVN 119
G GSSRE A L LK AG+S +IA+SF RIFYRNA+N+G+PL+ G ++++ +GD + VN
Sbjct: 64 GCGSSREHAPLGLKGAGISCVIAESFARIFYRNAINVGLPLIECKGISEKVNEGDELEVN 123
Query: 120 WETGEVRK--NGQTLQFEPLPGFLLEIVREGGILEFIRRR 157
ETGE++ G+ L+ + LP F++EI+ GG++ +++++
Sbjct: 124 LETGEIKNLTTGEVLKGQKLPEFMMEILEAGGLMPYLKKK 163
>sp|O27440|LE24_METTH PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7482020|pir||E69051 3-isopropylmalate dehydratase,
LeuC subunit - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2622497|gb|AAB85864.1| (AE000901)
3-isopropylmalate dehydratase, LeuC subunit
[Methanobacterium thermoautotrophicum]
Length = 162
Score = 161 bits (404), Expect = 4e-39
Identities = 77/157 (49%), Positives = 111/157 (70%), Gaps = 2/157 (1%)
Query: 5 GRVWKFWDNVSTDEITPGRYNLTKDPQELARIAFIEVRPEFAEKVRRGDVVVGGKNFGIG 64
G+VWKF D+V TD I PGRY + +DP++L + PEF KV+ GD +V GKNFG G
Sbjct: 3 GKVWKFPDDVDTDIIIPGRYLVMRDPEKLREHVMEGLDPEFPSKVKPGDFIVAGKNFGCG 62
Query: 65 SSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLL--IGDTDELEDGDVITVNWET 122
SSRE A LALK AG++ +IA+SF RIFYRNA+N+GIPLL G T++L +GD I V+ +
Sbjct: 63 SSREHAPLALKGAGIAAVIAESFARIFYRNAINVGIPLLEAPGITEKLNEGDEIEVDLDR 122
Query: 123 GEVRKNGQTLQFEPLPGFLLEIVREGGILEFIRRRGD 159
G + + F+ LP F++EI+ +GG++ +++++GD
Sbjct: 123 GVIIRGDDEFPFKKLPDFMVEILEKGGLIPYLKKKGD 159
>gi|11498237 3-isopropylmalate dehydratase, small subunit (leuD-1)
[Archaeoglobus fulgidus]
>gi|3122336|sp|O29626|LE23_ARCFU PUTATIVE
3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7482880|pir||E69328 3-isopropylmalate dehydratase,
small subunit (leuD-1) homolog - Archaeoglobus fulgidus
>gi|2649990|gb|AAB90610.1| (AE001061) 3-isopropylmalate
dehydratase, small subunit (leuD-1) [Archaeoglobus
fulgidus]
Length = 161
Score = 161 bits (402), Expect = 6e-39
Identities = 76/154 (49%), Positives = 111/154 (71%), Gaps = 3/154 (1%)
Query: 5 GRVWKFWDNVSTDEITPGRYNLTKDPQELARIAFIEVRPEFAEKVRRGDVVVGGKNFGIG 64
GR WKF D++ TD I G+Y + +P+ELA+ F +RPEFA++V++GD VV G+NFG G
Sbjct: 2 GRAWKFGDDIDTDVIIQGKYLVINEPEELAKHVFENLRPEFAKEVKKGDFVVAGENFGCG 61
Query: 65 SSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLL-IGDTDELEDGDVITVNWETG 123
SSRE A LALKA G+ +IAKS+ RIF+RNA+N+G+ +L +TD++EDGD + V++E G
Sbjct: 62 SSREHAPLALKATGIEAVIAKSYARIFFRNAINIGLRVLECKETDKIEDGDELEVDYEKG 121
Query: 124 EV--RKNGQTLQFEPLPGFLLEIVREGGILEFIR 155
+ + G+ PLP FL EI+ +GG++EF +
Sbjct: 122 VIYNKTKGEEYPINPLPDFLKEILEKGGLVEFAK 155
>sp|O26917|LE23_METTH PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7482021|pir||E69210 3-isopropylmalate dehydratase,
LeuD subunit - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2621919|gb|AAB85327.1| (AE000860)
3-isopropylmalate dehydratase, LeuD subunit
[Methanobacterium thermoautotrophicum]
Length = 170
Score = 148 bits (371), Expect = 3e-35
Identities = 73/152 (48%), Positives = 103/152 (67%), Gaps = 3/152 (1%)
Query: 2 ITTGRVWKFWDNVSTDEITPGRYNLTKDPQELARIAFIEVRPEFAEKVRRGDVVVGGKNF 61
I GRVW+F DNV TD I PGRY T ELA RPEFA +VR+GD++V G+NF
Sbjct: 4 IIRGRVWRFGDNVDTDMIIPGRYLRTFSLDELASHVMEGARPEFASQVRKGDIIVAGRNF 63
Query: 62 GIGSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLLIGDTDELEDGDVITVNWE 121
G GSSRE A +ALK AGV IIA+SF RIFYRNA+N+G+P+++ D +DGD ++++
Sbjct: 64 GCGSSREQAPVALKHAGVVAIIAESFARIFYRNAINIGLPVIMAKVD-ADDGDEVSIDLR 122
Query: 122 TGEVRK--NGQTLQFEPLPGFLLEIVREGGIL 151
+G++R G + +P ++L I+ +GG++
Sbjct: 123 SGQIRNLTAGSEYRMKPFNDYMLSILEDGGLV 154
>sp|Q9WZ25|LEUD_THEMA 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7462039|pir||A72363 3-isopropylmalate dehydratase,
small subunit - Thermotoga maritima (strain MSB8)
>gi|4981070|gb|AAD35640.1|AE001730_8 (AE001730)
3-isopropylmalate dehydratase, small subunit [Thermotoga
maritima]
Length = 166
Score = 148 bits (371), Expect = 3e-35
Identities = 75/153 (49%), Positives = 106/153 (69%), Gaps = 4/153 (2%)
Query: 5 GRVWKFWDNVSTDEITPGRYNLTKDPQELARIAFIEVRPEFAEKVR-RGDVVVGGKNFGI 63
G+V+ F DNV+TDEI P RY T DPQELA+ + RP F + +G ++V G+NFG
Sbjct: 7 GKVFVFGDNVNTDEIIPARYLNTSDPQELAKYCMEDARPGFGRRDDIKGSIIVAGENFGC 66
Query: 64 GSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLL-IGDTDELEDGDVITVNWET 122
GSSRE A +A+KAAG+S +IAKSF RIF+RNA+N+G+P++ + + DE E+GD+ V+ E
Sbjct: 67 GSSREHAPVAIKAAGISCVIAKSFARIFFRNAINIGLPIVELKEADEFEEGDIAEVDLEN 126
Query: 123 GEVRK--NGQTLQFEPLPGFLLEIVREGGILEF 153
G VR G+ + P P FL++I+ GG LE+
Sbjct: 127 GVVRNLTKGKEYRIRPYPEFLMKIMEAGGWLEY 159
>sp|Q58667|LE23_METJA PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|2127722|pir||F64458 3-isopropylmalate dehydratase
(EC 4.2.1.33) - Methanococcus jannaschii
>gi|1591907|gb|AAB99277.1| (U67568) 3-isopropylmalate
dehydratase (leuD) [Methanococcus jannaschii]
Length = 170
Score = 144 bits (360), Expect = 5e-34
Identities = 70/159 (44%), Positives = 103/159 (64%), Gaps = 2/159 (1%)
Query: 1 MITTGRVWKFWDNVSTDEITPGRYNLTKDPQELARIAFIEVRPEFAEKVRRGDVVVGGKN 60
MI GR KF D+V TD I PG Y T DP ELA + F +KV+ GDV+V G+N
Sbjct: 1 MIIKGRAHKFGDDVDTDAIIPGPYLRTTDPYELASHCMAGIDENFPKKVKEGDVIVAGEN 60
Query: 61 FGIGSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLLIGDTDELEDGDVITVNW 120
FG GSSRE A +A+K G+ +IAKSF RIFYRNA+N+G+ +I +TDE++DGD++ ++
Sbjct: 61 FGCGSSREQAVIAIKYCGIKAVIAKSFARIFYRNAINVGLIPIIANTDEIKDGDIVEIDL 120
Query: 121 ETGE--VRKNGQTLQFEPLPGFLLEIVREGGILEFIRRR 157
+ E + +T++ E G EI+ GG++ ++++R
Sbjct: 121 DKEEIVITNKNKTIKCETPKGLEREILAAGGLVNYLKKR 159
>pir||H75373 3-isopropylmalate dehydratase, small subunit - Deinococcus
radiodurans (strain R1)
>gi|6459381|gb|AAF11175.1|AE002005_4 (AE002005)
3-isopropylmalate dehydratase, small subunit
[Deinococcus radiodurans]
Length = 177
Score = 143 bits (356), Expect = 2e-33
Identities = 75/155 (48%), Positives = 106/155 (68%), Gaps = 2/155 (1%)
Query: 6 RVWKFWDNVSTDEITPGRYNLTKDPQELAR-IAFIEVRPEFAEKVRRGDVVVGGKNFGIG 64
R+WKF D+V+TD+I PG++ +++ + AF VRPEFA +V+ GDV++GG+N+G+G
Sbjct: 3 RIWKFGDSVNTDDILPGKFAPFMAGEDVFQTFAFHYVRPEFAAQVQPGDVLIGGRNWGLG 62
Query: 65 SSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLLIGD-TDELEDGDVITVNWETG 123
SSRE A ALK + GI+A SF RI YRN +NLGIP D T+ LEDGD +T++ +TG
Sbjct: 63 SSREYAPQALKKLHIGGIVAPSFARIHYRNLLNLGIPAFEYDLTELLEDGDEVTLDAQTG 122
Query: 124 EVRKNGQTLQFEPLPGFLLEIVREGGILEFIRRRG 158
+ T+Q P P FL E ++EG ILEF ++ G
Sbjct: 123 LLTYADGTVQLPPPPEFLREALKEGSILEFFKKHG 157
>pir||E70421 3-isopropylmalate dehydratase - Aquifex aeolicus
>gi|2983802|gb|AAC07359.1| (AE000738) 3-isopropylmalate
dehydratase [Aquifex aeolicus]
Length = 219
Score = 142 bits (355), Expect = 2e-33
Identities = 73/158 (46%), Positives = 105/158 (66%), Gaps = 5/158 (3%)
Query: 5 GRVWKFWDNVSTDEITPGRYNLTKDPQELARIAFIEVR-PEFAEKVRRGDVVVGGKNFGI 63
GRVWKF DNV TD+I P RY T DP ELA+ + PEFA++ + GD++V GKNFG
Sbjct: 58 GRVWKFGDNVDTDQIIPARYLNTSDPYELAKHVMEDSEHPEFAKEHKEGDIIVAGKNFGS 117
Query: 64 GSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLLIGD--TDELEDGDVITVNWE 121
GSSRE A +A+K +GV +IAKSF RIF+RNA+N+G+P++ DE+E GD I V+ E
Sbjct: 118 GSSREHAPIAIKYSGVPVVIAKSFARIFFRNAINIGLPIVEAPEAVDEIEHGDEIEVDLE 177
Query: 122 TGEVR--KNGQTLQFEPLPGFLLEIVREGGILEFIRRR 157
G ++ + G+ Q P L +I++ GG++ + + +
Sbjct: 178 KGVIKNLRTGKEYQATKFPKELQDILKAGGLMAYAKEK 215
>sp|O67399|LEUD_AQUAE 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
Length = 168
Score = 142 bits (355), Expect = 2e-33
Identities = 73/158 (46%), Positives = 105/158 (66%), Gaps = 5/158 (3%)
Query: 5 GRVWKFWDNVSTDEITPGRYNLTKDPQELARIAFIEVR-PEFAEKVRRGDVVVGGKNFGI 63
GRVWKF DNV TD+I P RY T DP ELA+ + PEFA++ + GD++V GKNFG
Sbjct: 7 GRVWKFGDNVDTDQIIPARYLNTSDPYELAKHVMEDSEHPEFAKEHKEGDIIVAGKNFGS 66
Query: 64 GSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLLIGD--TDELEDGDVITVNWE 121
GSSRE A +A+K +GV +IAKSF RIF+RNA+N+G+P++ DE+E GD I V+ E
Sbjct: 67 GSSREHAPIAIKYSGVPVVIAKSFARIFFRNAINIGLPIVEAPEAVDEIEHGDEIEVDLE 126
Query: 122 TGEVR--KNGQTLQFEPLPGFLLEIVREGGILEFIRRR 157
G ++ + G+ Q P L +I++ GG++ + + +
Sbjct: 127 KGVIKNLRTGKEYQATKFPKELQDILKAGGLMAYAKEK 164
>gb|AAK42607.1| 3-isopropylmalate dehydratase, small subunit (isopropylmalate
isomerase) (alpha IPM isomerase) (IPMI) (leuD)
[Sulfolobus solfataricus]
Length = 165
Score = 140 bits (350), Expect = 8e-33
Identities = 71/158 (44%), Positives = 104/158 (64%), Gaps = 2/158 (1%)
Query: 1 MITTGRVWKFWDNVSTDEITPGRYNLTKDPQELARIAFIEVRPEFAEKVRRGDVVVGGKN 60
MI G V KF D + TD I P RY DPQ LA+ + PEF +K +G ++V GK
Sbjct: 1 MIIEGPVIKFGDKIDTDIIIPARYLKYTDPQYLAQHVMEPLDPEFYKKASKGVIIVAGKV 60
Query: 61 FGIGSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPL--LIGDTDELEDGDVITV 118
FG+GSSRE AA+ALKAAGV ++A+SF RIFYRNA+N G+P+ L T E+++ + +
Sbjct: 61 FGMGSSREQAAIALKAAGVKAVVAESFARIFYRNAINNGLPVITLPNSTKEIDENSYVKI 120
Query: 119 NWETGEVRKNGQTLQFEPLPGFLLEIVREGGILEFIRR 156
+ ETGE+ + L+ + + G LEI++ GGI+E++++
Sbjct: 121 DVETGEILVGNKVLKGKGITGMALEILQAGGIMEYLKK 158
>dbj|BAA74763.1| (AB017109) HacB [Thermus thermophilus]
Length = 163
Score = 136 bits (339), Expect = 1e-31
Identities = 71/156 (45%), Positives = 102/156 (64%), Gaps = 3/156 (1%)
Query: 6 RVWKFWDNVSTDEITPGRYNLTKDPQELARI-AFIEVRPEFAEKVRRGDVVVGGKNFGIG 64
RVWKF D ++TD+I PG+Y ++ + AF +RPEFA++VR GD++V G+N G+G
Sbjct: 3 RVWKFGDQINTDDILPGKYAPFMVGEDRFHLYAFAHLRPEFAKEVRPGDILVFGRNAGLG 62
Query: 65 SSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLLIGD--TDELEDGDVITVNWET 122
SSRE A ALK GV IIAKS+ RIF+RN VNLGI + D LEDGD + ++ E+
Sbjct: 63 SSREYAPEALKRLGVRAIIAKSYARIFFRNLVNLGIVPFESEEVVDALEDGDEVELDLES 122
Query: 123 GEVRKNGQTLQFEPLPGFLLEIVREGGILEFIRRRG 158
G + + + P P FLLE ++EG +L++ ++ G
Sbjct: 123 GVLTRGEERFALRPPPPFLLEALKEGSLLDYYKKHG 158
>pir||F75355 3-isopropylmalate dehydratase, small subunit - Deinococcus
radiodurans (strain R1)
>gi|6459552|gb|AAF11332.1|AE002019_5 (AE002019)
3-isopropylmalate dehydratase, small subunit
[Deinococcus radiodurans]
Length = 208
Score = 116 bits (288), Expect = 1e-25
Identities = 61/153 (39%), Positives = 96/153 (61%), Gaps = 4/153 (2%)
Query: 12 DNVSTDEITPGRYNLTKDPQELARIAFIEVRPEFAEKVRRGDVVVGGKNFGIGSSRESAA 71
D+++TDEI P R+ T ELA+ A + +F +V+ GD++V G +FG GSSRE A
Sbjct: 10 DHINTDEIIPARHLTTDVESELAKYAMEDYDKDFVRRVQPGDIIVAGADFGCGSSREHAV 69
Query: 72 LALKAAGVSGIIAKSFGRIFYRNAVNLGIPLL--IGDTDELEDGDVITVNWETGEVR--K 127
AL+ AGVS +IA +F RI+YRN++N G L G T+ +DG+ ++ + G +R +
Sbjct: 70 WALRGAGVSAVIAPNFARIYYRNSINNGFLALECEGITELFQDGEEAELDLKGGTIRNPR 129
Query: 128 NGQTLQFEPLPGFLLEIVREGGILEFIRRRGDL 160
G+ L F P+P F L++ + GG LE+++ D+
Sbjct: 130 TGKELSFVPVPQFALDVQKAGGWLEYMKAGEDV 162
>sp|Q92412|LYS4_EMENI HOMOACONITASE PRECURSOR (HOMOACONITATE HYDRATASE)
>gi|1628374|emb|CAA67943.1| (X99624) homoaconitase
[Aspergillus nidulellus]
Length = 775
Score = 88.9 bits (217), Expect = 3e-17
Identities = 59/167 (35%), Positives = 89/167 (52%), Gaps = 23/167 (13%)
Query: 12 DNVSTDEITPGRYNLTKD--PQELARIAFIEVRPEFAEKVRRGDVVVGGKNFGIGSSRES 69
DNV+TD I PG+Y D P+ +AR+ PEF+ + GD++V G NFG GSSRE
Sbjct: 604 DNVNTDGIYPGKYTYQDDVPPETMARVCMENYDPEFSTTAKEGDILVSGFNFGCGSSREQ 663
Query: 70 AALALKAAGVSGIIAKSFGRIFYRNAVN-----LGIPLLIGDTDE-LEDGDVI---TVNW 120
AA A+ A + +++ SFG IF RN++N L +P L+ E GD + W
Sbjct: 664 AATAILAKKIPLVVSGSFGNIFSRNSINNALMGLEVPRLVNRLRETFGSGDKVLTRRTGW 723
Query: 121 E-TGEVRKNGQTLQFEP-----------LPGFLLEIVREGGILEFIR 155
T +VRK+ +Q P LP + EI+ +GG+ ++++
Sbjct: 724 TLTWDVRKSQIEVQEGPGGPKWTHKVGELPPNVQEIIAKGGLEKWVK 770
>emb|CAA89720.1| (Z49701) unknown [Saccharomyces cerevisiae]
Length = 375
Score = 84.3 bits (205), Expect = 8e-16
Identities = 50/119 (42%), Positives = 67/119 (56%), Gaps = 7/119 (5%)
Query: 12 DNVSTDEITPGRYNLTKD--PQELARIAFIEVRPEFAEKVRRGDVVVGGKNFGIGSSRES 69
DN++TD I PG+Y D +++A++ EF KV GD+VV G NFG GSSRE
Sbjct: 204 DNINTDGIYPGKYTYQDDVPKEKMAQVCMENYDAEFRTKVHPGDIVVSGFNFGTGSSREQ 263
Query: 70 AALALKAAGVSGIIAKSFGRIFYRNAVN-----LGIPLLIGDTDELEDGDVITVNWETG 123
AA AL A G++ +++ SFG IF RN++N L IP LI E G + TG
Sbjct: 264 AATALLAKGINLVVSGSFGNIFSRNSINNALLTLEIPALIKKLREKYQGAPKELTRRTG 322
>gi|6320440 homoaconitase; Lys4p [Saccharomyces cerevisiae]
>gi|1346476|sp|P49367|LYS4_YEAST HOMOACONITASE PRECURSOR
(HOMOACONITATE HYDRATASE) >gi|2131287|pir||S61067
homoaconitate hydratase (EC 4.2.1.36) precursor - yeast
(Saccharomyces cerevisiae) >gi|1089840|emb|CAA63764.1|
(X93502) homoaconitase [Saccharomyces cerevisiae]
>gi|1197581|gb|AAA88902.1| (U46154) lysine biosynthesis
enzyme homoaconitase [Saccharomyces cerevisiae]
Length = 693
Score = 84.3 bits (205), Expect = 8e-16
Identities = 50/119 (42%), Positives = 67/119 (56%), Gaps = 7/119 (5%)
Query: 12 DNVSTDEITPGRYNLTKD--PQELARIAFIEVRPEFAEKVRRGDVVVGGKNFGIGSSRES 69
DN++TD I PG+Y D +++A++ EF KV GD+VV G NFG GSSRE
Sbjct: 522 DNINTDGIYPGKYTYQDDVPKEKMAQVCMENYDAEFRTKVHPGDIVVSGFNFGTGSSREQ 581
Query: 70 AALALKAAGVSGIIAKSFGRIFYRNAVN-----LGIPLLIGDTDELEDGDVITVNWETG 123
AA AL A G++ +++ SFG IF RN++N L IP LI E G + TG
Sbjct: 582 AATALLAKGINLVVSGSFGNIFSRNSINNALLTLEIPALIKKLREKYQGAPKELTRRTG 640
>gb|AAC64298.1| (AC004450) 3-isopropylmalate dehydratase, small subunit
[Arabidopsis thaliana]
Length = 251
Score = 78.0 bits (189), Expect = 6e-14
Identities = 52/167 (31%), Positives = 88/167 (52%), Gaps = 21/167 (12%)
Query: 12 DNVSTDEITPGRYN--LTKDPQELARI---AFIEVRPEFAEK-VRRGD------VVVGGK 59
DN+ TD+I P + + +P+E ++ A + + + E+ V+ G+ +++GG+
Sbjct: 79 DNIDTDQIIPAEFLTLVPSNPEEYEKLGSYALVGLPASYKERFVQPGEMKTKYSIIIGGE 138
Query: 60 NFGIGSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLG----IPLLIGDTDELEDGDV 115
NFG GSSRE A + L AAG ++A+S+ RIF+RN+V G + + DE GDV
Sbjct: 139 NFGCGSSREHAPVCLGAAGAKAVVAQSYARIFFRNSVATGEVYPLDSEVRVCDECTTGDV 198
Query: 116 ITVNWETGEV----RKNGQTLQFEPLPGFLLEIVREGGILEFIRRRG 158
TV G+ G+ + +P+ G ++ GGI + R+ G
Sbjct: 199 ATVELREGDSILINHTTGKEYKLKPI-GDAGPVIDAGGIFAYARKAG 244
>pir||T47781 3-isopropylmalate dehydratase-like protein (small subunit) -
Arabidopsis thaliana >gi|7529742|emb|CAB86927.1|
(AL163527) 3-isopropylmalate dehydratase-like protein
(small subunit) [Arabidopsis thaliana]
Length = 253
Score = 77.3 bits (187), Expect = 1e-13
Identities = 57/174 (32%), Positives = 89/174 (50%), Gaps = 34/174 (19%)
Query: 12 DNVSTDEITPGRY-----NLTKDPQELARIAFIEVRPEF--------AEKVRRGDVVVGG 58
DN+ TD+I P Y ++ +D ++L A + P+F E + V++GG
Sbjct: 82 DNIDTDQIIPAEYGTLIPSIPEDREKLGSFA-LNGLPKFYNERFVVPGEMKSKYSVIIGG 140
Query: 59 KNFGIGSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLG--IPL--LIGDTDELEDGD 114
NFG GSSRE A + L AAG ++A+S+ RIF+RN V G PL + DE + GD
Sbjct: 141 DNFGCGSSREHAPVCLGAAGAKAVVAESYARIFFRNCVATGEIFPLESEVRICDECKTGD 200
Query: 115 VITVNWETGEVRKNGQTL----------QFEPLPGFLLEIVREGGILEFIRRRG 158
V+T+ E +++G +L + +PL G ++ GGI + R+ G
Sbjct: 201 VVTI-----EHKEDGSSLLINHTTRKEYKLKPL-GDAGPVIDAGGIFAYARKAG 248
>pir||T38665 probable homoaconitate hydratase (EC 4.2.1.36) - fission yeast
(Schizosaccharomyces pombe) >gi|5706517|emb|CAB52279.1|
(AL109739) putative homoaconitate hydratase (EC
4.2.1.36) [Schizosaccharomyces pombe]
Length = 721
Score = 76.1 bits (184), Expect = 2e-13
Identities = 37/88 (42%), Positives = 55/88 (62%), Gaps = 2/88 (2%)
Query: 12 DNVSTDEITPGRYNLTKD--PQELARIAFIEVRPEFAEKVRRGDVVVGGKNFGIGSSRES 69
DN++TD I PGRY D +E+A++ EF +K ++ D++V G NFG GSSRE
Sbjct: 549 DNLNTDGIYPGRYTYRDDITKEEMAKVCMENYDSEFGKKTKKDDILVSGFNFGTGSSREQ 608
Query: 70 AALALKAAGVSGIIAKSFGRIFYRNAVN 97
AA A+ + G+ ++ SF IF RN++N
Sbjct: 609 AATAILSRGIPLVVGGSFSDIFKRNSIN 636
>pir||F70453 aconitase - Aquifex aeolicus >gi|2984078|gb|AAC07617.1| (AE000756)
aconitase [Aquifex aeolicus]
Length = 659
Score = 71.8 bits (173), Expect = 4e-12
Identities = 54/175 (30%), Positives = 84/175 (47%), Gaps = 34/175 (19%)
Query: 12 DNVSTDEITPGRYN---LTKDPQELARIAFIEVRPEFAEKVRR------GDVVVGGKNFG 62
DN++TD I P L + ++ + V PEF ++++ +++GG+N+G
Sbjct: 483 DNITTDHIMPAGAKILPLRSNIYAISEYVYHYVDPEFVPRMKKLKEKGKAGIIIGGENYG 542
Query: 63 IGSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGIPLL------------IGDTDEL 110
GSSRE AALA + GV +IAKSF RI + N VN G+ L +GD E+
Sbjct: 543 QGSSREHAALAPRFLGVRAVIAKSFARIHHANLVNFGVVPLEFKNKEDYDKFSLGDEIEI 602
Query: 111 --------EDGDVITVNWETGEVRKNGQTLQFEPLPGFLLEIVREGGILEFIRRR 157
+ D++ +N TGE + L L EI+ GG L +I+ +
Sbjct: 603 PNLIERLKKGEDILVINKTTGE-----EILCTYNLTPVQKEILIAGGRLNYIKNK 652
>gb|AAC64299.1| (AC004450) 3-isopropylmalate dehydratase, small subunit
[Arabidopsis thaliana]
Length = 256
Score = 69.5 bits (167), Expect = 2e-11
Identities = 54/167 (32%), Positives = 85/167 (50%), Gaps = 21/167 (12%)
Query: 12 DNVSTDEITPGRYNLT--KDPQELARIAF--IEVRPEF--------AEKVRRGDVVVGGK 59
DN+ TD+I P T + QE IA + P+F E + +++GG+
Sbjct: 85 DNIDTDQIIPAGAACTFPSNQQERDEIAAHALSGLPDFHKTRFIEPGENRSKYSIIIGGE 144
Query: 60 NFGIGSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLG--IPL--LIGDTDELEDGDV 115
NFG GSSRE A + L AAG I+A+S+ RIF+RN+V G PL + +E + GD
Sbjct: 145 NFGCGSSREHAPVCLGAAGAKAIVAESYARIFFRNSVATGEVFPLESEVRVCEECKTGDT 204
Query: 116 ITVNW-ETGEVRKN---GQTLQFEPLPGFLLEIVREGGILEFIRRRG 158
+T+ ++G + N G+ + + + G ++ GGI + R G
Sbjct: 205 VTIELSDSGGLLTNHTTGKNYKLKSI-GDAGPVIDAGGIFAYARMMG 250
>gb|AAG20620.1| (AE005132) aconitase; Can [Halobacterium sp. NRC-1]
Length = 661
Score = 67.5 bits (162), Expect = 8e-11
Identities = 45/130 (34%), Positives = 67/130 (50%), Gaps = 8/130 (6%)
Query: 9 KFWDNVSTDEITPGRYNLTK---DPQELARIAFIEVRPEFAEKVRRGD--VVVGGKNFGI 63
K DN++TD I P ++ K + + L+ + FAE+ + D V+V G+N+G
Sbjct: 481 KMQDNITTDHIIPATSDILKFRSNIERLSEFTLSRIDDTFAERAKAADSGVLVAGENYGQ 540
Query: 64 GSSRESAALALKAAGVSGIIAKSFGRIFYRNAVNLGI-PLLIGDTD--ELEDGDVITVNW 120
GSSRE AA+ GV ++A+SF RI N N GI PL I + +E GD + V
Sbjct: 541 GSSREHAAMCPMFLGVESVLAQSFARIHKANLFNFGILPLNIDEETYARIEQGDDVEVVD 600
Query: 121 ETGEVRKNGQ 130
+ +GQ
Sbjct: 601 DVAAAVADGQ 610
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.321 0.143 0.425
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63001498
Number of Sequences: 2977
Number of extensions: 2564142
Number of successful extensions: 5955
Number of sequences better than 1.0e-10: 26
Number of HSP's better than 0.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 5905
Number of HSP's gapped (non-prelim): 26
length of query: 163
length of database: 189,106,746
effective HSP length: 51
effective length of query: 112
effective length of database: 158,583,909
effective search space: 17761397808
effective search space used: 17761397808
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 162 (67.5 bits)