BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0289 (leuB-2) DE:3-isopropylmalate dehydrogenase (leuB)
(346 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||B75160 3-isopropylmalate dehydrogenase (leub) PAB0289 - Pyr... 691 0.0
pir||E71180 probable 3-isopropylmalate dehydrogenase - Pyrococcu... 489 e-137
pir||A69113 isocitrate dehydrogenase - Methanobacterium thermoau... 247 1e-64
sp|Q58991|IDH_METJA ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCCIN... 245 7e-64
pir||C64499 isocitrate dehydrogenase (EC 1.1.1.-) - Methanococcu... 243 3e-63
pir||E75368 probable isocitrate dehydrogenase - Deinococcus radi... 224 2e-57
gi|11498236 3-isopropylmalate dehydrogenase (leuB) [Archaeoglobu... 220 2e-56
sp|Q58130|LEU3_METJA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 214 1e-54
pir||H64389 isocitrate dehydrogenase (NADP+) (EC 1.1.1.42) - Met... 214 1e-54
pir||D75045 3-isopropylmalate dehydrogenase (leub) PAB2424 - Pyr... 213 2e-54
gi|6322097 Homo-isocitrate dehydrogenase; Lys12p [Saccharomyces ... 213 2e-54
sp|O66607|LEU3_AQUAE 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 210 2e-53
dbj|BAB06776.1| (AP001517) 3-isopropylmalate dehydrogenase [Baci... 204 1e-51
sp|O27441|LEU3_METTH 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 204 1e-51
sp|Q02143|LEU3_LACLA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 202 6e-51
sp|P24015|LEU3_LEPIN 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 201 1e-50
pir||A82539 isocitrate dehydrogenase XF2596 [imported] - Xylella... 199 5e-50
sp|P50455|LEU3_SULS7 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 198 7e-50
pir||T38621 probable isopropyl malate dehydrogenase - fission ye... 198 9e-50
sp|P12010|LEU3_BACCO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 192 7e-48
gb|AAA22554.1| (M33099) 3-isopropylmalate dehydrogenase [Bacillu... 192 7e-48
sp|P43860|LEU3_HAEIN 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 192 7e-48
sp|P24098|LEU3_THEAQ 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 191 9e-48
dbj|BAA88179.1| (AP000836) ESTs C19133(E10008),AU058199(E50136),... 188 7e-47
sp|P94631|LEU3_CORGL 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 187 2e-46
sp|P05644|LEU3_BACCA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 187 2e-46
pir||T03406 probable isocitrate dehydrogenase (NAD+) (EC 1.1.1.4... 185 6e-46
pir||T48350 3-isopropylmalate dehydrogenase-like protein - Arabi... 185 8e-46
pdb|1XAC| Chimera Isopropylmalate Dehydrogenase Between Bacill... 185 8e-46
pdb|1OSJ|A Chain A, Structure Of 3-Isopropylmalate Dehydrogenase... 185 8e-46
pdb|1DR0|A Chain A, Structure Of Modified 3-Isopropylmalate Dehy... 184 1e-45
pdb|1IDM| 3-Isopropylmalate Dehydrogenase, Loop-Deleted Chimera 184 1e-45
sp|P18869|LEU3_SCHPO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 183 2e-45
pdb|1DPZ|A Chain A, Stucture Of Modified 3-Isopropylmalate Dehyd... 183 2e-45
pdb|1DR8|A Chain A, Structure Of Modified 3-Isopropylmalate Dehy... 183 2e-45
pdb|1G2U|A Chain A, The Structure Of The Mutant, A172v, Of 3-Iso... 183 2e-45
pdb|1GC8|A Chain A, The Crystal Structure Of Thermus Thermophilu... 183 2e-45
sp|P00351|LEU3_THETH 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 183 3e-45
pir||S41223 3-isopropylmalate dehydrogenase - Thermus aquaticus ... 183 3e-45
pdb|1WAL|A Chain A, 3-Isopropylmalate Dehydrogenase (Ipmdh) Muta... 182 6e-45
pdb|1GC9|A Chain A, The Crystal Structure Of Thermus Thermophilu... 182 7e-45
gb|AAK41021.1| 3-isopropylmalate dehydrogenase (leuB) [Sulfolobu... 181 1e-44
emb|CAB57580.1| (Y18930) 3-isopropylmalate dehydrogenase [Sulfol... 181 1e-44
pir||A26522 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Baci... 180 2e-44
sp|P05645|LEU3_BACSU 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 180 2e-44
pir||G75355 3-isopropylmalate dehydrogenase - Deinococcus radiod... 180 2e-44
sp|O34296|TTC3_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC' (TDH)... 180 3e-44
sp|O34295|TTC5_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC' (TDH)... 179 5e-44
gb|AAF23254.1|AC015985_12 (AC015985) putative isocitrate dehydro... 178 8e-44
sp|Q44471|TTC1_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC (TDH) ... 178 1e-43
pir||G82070 3-isopropylmalate dehydrogenase VC2491 [imported] - ... 178 1e-43
sp|P33197|IDH_THETH ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCCIN... 177 1e-43
pir||A43934 isocitrate dehydrogenase (NADP+) (EC 1.1.1.42) - The... 177 1e-43
sp|P70792|TTC4_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC' (TDH)... 176 4e-43
pir||T50386 probable isocitrate dehydrogenase (NAD+) (EC 1.1.1.4... 176 4e-43
sp|P70787|TTC2_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC (TDH) ... 175 5e-43
pir||S30897 3-isopropylmalate dehydrogenase (EC 1.1.1.85) precur... 175 7e-43
sp|P94929|LEU3_MYCBO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 175 9e-43
sp|P95313|LEU3_MYCTU 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 175 9e-43
sp|O33117|LEU3_MYCLE 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 174 2e-42
gb|AAF48965.1| (AE003512) CG12233 gene product [Drosophila melan... 173 2e-42
sp|P29696|LEU3_SOLTU 3-ISOPROPYLMALATE DEHYDROGENASE PRECURSOR (... 173 4e-42
dbj|BAB04789.1| (AP001510) tartrate dehydrogenase [Bacillus halo... 172 5e-42
sp|P37412|LEU3_SALTY 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 172 6e-42
sp|P30125|LEU3_ECOLI 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 171 8e-42
pir||A64729 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Esch... 171 8e-42
sp|Q9WZ26|LEU3_THEMA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 170 2e-41
pir||S20606 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Salm... 169 3e-41
sp|P34738|LEU3_NEUCR 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 169 4e-41
prf||2004294A beta isopropylmalate dehydrogenase [Neurospora cra... 169 4e-41
sp|P76251|TTUC_ECOLI PROBABLE TARTRATE DEHYDROGENASE (TDH) >gi|7... 169 4e-41
pir||D83255 3-isopropylmalate dehydrogenase PA3118 [imported] - ... 169 4e-41
sp|Q01987|LEU3_CANBO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 169 5e-41
sp|Q51945|TTUC_PSEPU TARTRATE DEHYDROGENASE (TDH) >gi|1084041|pi... 168 7e-41
sp|Q51375|LEU3_PSEAE 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 168 7e-41
gb|AAB91428.1| (U86815) beta-isopropylmalate dehydrogenase [Expr... 168 9e-41
sp|P31958|LEU3_CLOPA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 168 1e-40
gb|AAB49959.1| (U70024) LEU2 [Cloning vector pGAD-C1] >gi|159584... 168 1e-40
gb|AAC69608.1| (AF045153) NAD-dependent isocitrate dehydrogenase... 167 2e-40
sp|Q9ZDR0|IDH_RICPR ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCCIN... 167 2e-40
pir||DEBYI 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - yeast... 166 3e-40
emb|CAA60637.1| (X87172) NAD+-isocitrate dehydrogenase, alpha su... 166 3e-40
sp|Q28480|IDHA_MACFA ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPH... 166 3e-40
gi|10383772 beta-IPM (isopropylmalate) dehydrogenase; Leu2p [Sac... 166 5e-40
sp|P04173|LEU3_YEAST 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 164 1e-39
sp|P54354|LEU3_BACFR 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 164 1e-39
pir||T37546 probable isocitrate dehydrogenase (NAD+) (EC 1.1.1.4... 164 1e-39
gb|AAD56647.1|AF178973_2 (AF178973) dLEU2 [Cloning vector pCS512... 164 1e-39
sp|P34733|LEU3_PICAN 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 164 2e-39
gi|5031777 isocitrate dehydrogenase 3 (NAD+) alpha [Homo sapiens... 164 2e-39
gi|6324709 NAD+-dependent isocitrate dehydrogenase; Idh2p [Sacch... 163 2e-39
sp|P41563|IDHA_BOVIN ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPH... 163 2e-39
sp|P73960|LEU3_SYNY3 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 163 2e-39
sp|Q56268|LEU3_THIFE 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 163 3e-39
sp|P23390|LEU3_KLULA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 162 7e-39
sp|P18120|LEU3_YARLI 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 162 7e-39
gb|AAA62618.1| (L14756) 3-isopropylmalate dehydrogenase [Cloning... 162 7e-39
gb|AAC53643.1| (U38900) 3-isopropylmalate dehydrogenase [Cloning... 161 1e-38
gb|AAG31928.1|AF200469_2 (AF200469) leuB [Buchnera aphidicola] 161 2e-38
gi|10956836 3-isopropylmalate dehydrogenase [Sphingomonas aromat... 160 3e-38
gb|AAC69609.1| (AF045154) NAD-dependent isocitrate dehydrogenase... 159 4e-38
pir||E82564 3-isopropylmalate dehydrogenase XF2372 [imported] - ... 159 4e-38
emb|CAB99456.1| (AJ292544) beta-isopropylmalate dehydrogenase [Z... 159 4e-38
gi|11498255 isocitrate dehydrogenase, NADP (icd) [Archaeoglobus ... 159 5e-38
pir||A72658 probable isocitrate dehydrogenase APE0689 - Aeropyru... 159 6e-38
pir||B81270 3-isopropylmalate dehydrogenase (EC 1.1.1.85) Cj1718... 159 6e-38
pir||E81130 3-isopropylmalate dehydrogenase NMB1031 [imported] -... 158 1e-37
emb|CAA72702.1| (Y11966) 3-isopropylmalate dehydrogenase [Buchne... 157 1e-37
dbj|BAB08299.1| (AB007650) 3-isopropylmalate dehydrogenase [Arab... 157 1e-37
gb|AAG31402.1| (AF197456) 3-isopropylmaltate dehydrogenase [Buch... 157 1e-37
sp|P24404|LEU3_AGRTU 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 157 2e-37
sp|O14429|LEU3_CANGA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 157 2e-37
gi|10957108 3-isopropylmalate dehydrogenase [Buchnera sp. APS] >... 157 2e-37
gb|AAG31378.1| (AF197448) 3-isopropylmaltate dehydrogenase [Buch... 157 2e-37
gb|AAG31405.1| (AF197457) 3-isopropylmaltate dehydrogenase [Buch... 157 2e-37
gi|6324291 alpha-4-beta-4 subunit of mitochondrial isocitrate de... 157 2e-37
pir||E81836 probable 3-isopropylmalate dehydrogenase (EC 1.1.1.8... 157 2e-37
gi|10954451 isopropylmalate dehydrogenase [Buchnera aphidicola] ... 156 3e-37
gb|AAG31384.1| (AF197450) 3-isopropylmaltate dehydrogenase [Buch... 156 3e-37
sp|O60027|LEU3_ASHGO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 156 4e-37
sp|O59930|LEU3_PHACH 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 155 5e-37
gb|AAD21684.1| (AC004793) Strong similarity to gb|X59970 3-isopr... 155 5e-37
gb|AAG31387.1| (AF197451) 3-isopropylmaltate dehydrogenase [Buch... 155 5e-37
gb|AAG31393.1| (AF197453) 3-isopropylmaltate dehydrogenase [Buch... 155 5e-37
gb|AAG31399.1| (AF197455) 3-isopropylmaltate dehydrogenase [Buch... 155 5e-37
sp|Q9Y897|LEU3_MYCGR 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 155 7e-37
gb|AAG31390.1| (AF197452) 3-isopropylmaltate dehydrogenase [Buch... 155 7e-37
gb|AAB63461.1| (AF009036) NAD(+)-isocitrate dehydrogenase subuni... 155 7e-37
emb|CAB72262.1| (AJ271154) 3-isopropylmalate dehydrogenase [Phae... 155 9e-37
emb|CAA71268.1| (Y10216) hypothetical 3-isopropylmalate dehydrog... 154 1e-36
sp|P87256|LE3A_ASPNG 3-ISOPROPYLMALATE DEHYDROGENASE A (BETA-IPM... 154 1e-36
sp|O77784|IDHB_BOVIN ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT BETA... 154 2e-36
sp|P41766|LEU3_KLUMA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 153 2e-36
sp|P08791|LEU3_PICJA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 153 2e-36
sp|P48572|LEU3_BUCRP 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 153 2e-36
emb|CAC09449.1| (AL442090) hypothetical protein [Homo sapiens] 153 3e-36
gi|11431719 isocitrate dehydrogenase 3 (NAD+) alpha [Homo sapiens] 153 3e-36
sp|P29102|LEU3_BRANA 3-ISOPROPYLMALATE DEHYDROGENASE PRECURSOR (... 152 6e-36
sp|P41019|LEU3_BACME 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 152 6e-36
gb|AAC83166.1| (AF090321) NAD(+)-isocitrate dehydrogenase subuni... 152 8e-36
gb|AAG31396.1| (AF197454) 3-isopropylmaltate dehydrogenase [Buch... 152 8e-36
sp|P56933|LEU3_BUCAI 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 151 1e-35
sp|Q12545|LEU3_CEPAC 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 151 1e-35
sp|Q28479|IDHB_MACFA ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT BETA... 151 1e-35
gb|AAB81675.1| (AC002354) putative NAD+ dependent isocitrate deh... 151 1e-35
sp|Q93714|IDHA_CAEEL PROBABLE ISOCITRATE DEHYDROGENASE [NAD] SUB... 151 1e-35
gb|AAC49965.1| (U81994) NAD+ dependent isocitrate dehydrogenase ... 150 2e-35
gi|5901982 isocitrate dehydrogenase 3 (NAD+) beta [Homo sapiens]... 150 2e-35
pir||T06131 isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) chain ... 150 2e-35
emb|CAA74776.1| (Y14431) NAD-dependent isocitrate dehydrogenase ... 150 2e-35
pir||T13147 isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) beta c... 150 2e-35
gb|AAD55084.1| (AF176568) isocitrate dehydrogenase gamma subunit... 150 2e-35
emb|CAC01442.1| (AL049712) dJ686C3.1.2 (isocitrate dehydrogenase... 150 2e-35
gi|11421208 isocitrate dehydrogenase 3 (NAD+) beta [Homo sapiens] 150 2e-35
pir||S48225 3-isopropylmalate dehydrogenase (EC 1.1.1.85) (allel... 150 3e-35
gb|AAG31381.1| (AF197449) 3-isopropylmaltate dehydrogenase [Buch... 150 3e-35
gb|AAD09339.1| (AF023265) NAD+-specific isocitrate dehydrogenase... 149 4e-35
emb|CAC01443.1| (AL049712) dJ686C3.1.1 (isocitrate dehydrogenase... 149 4e-35
gi|10954459 3-isopropylmalate dehydrogenase [Buchnera aphidicola... 147 2e-34
sp|Q00412|LEU3_SPIPL 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 142 5e-33
pir||A44851 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Spir... 142 5e-33
pir||T21799 hypothetical protein F35G12.2 - Caenorhabditis elega... 141 9e-33
sp|P41564|IDHG_MACFA ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT GAMM... 139 3e-32
gi|4758582 isocitrate dehydrogenase 3 (NAD+) gamma [Homo sapiens... 139 4e-32
gi|11419927 isocitrate dehydrogenase 3 (NAD+) gamma [Homo sapiens] 139 4e-32
dbj|BAA13177.2| (D86855) isocitrate dehydrogenase [Caldococcus n... 138 1e-31
pir||T44658 isocitrate dehydrogenase (NADP+) (EC 1.1.1.42) [vali... 137 2e-31
emb|CAA52225.1| (X74125) NAD+-isocitrate dehydrogenase, gamma su... 136 5e-31
pir||S39064 isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) gamma ... 136 5e-31
gi|6680345 isocitrate dehydrogenase 3 (NAD+), gamma [Mus musculu... 136 5e-31
sp|P41565|IDHG_RAT ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT GAMMA,... 136 5e-31
sp|P87257|LE3B_ASPNG 3-ISOPROPYLMALATE DEHYDROGENASE B (BETA-IPM... 134 1e-30
gb|AAF56494.1| (AE003753) CG5028 gene product [Drosophila melano... 132 4e-30
sp|P42958|YCSA_BACSU HYPOTHETICAL 33.1 KD PROTEIN IN MTLD-SIPU I... 132 6e-30
gb|AAC83168.1| (AF090323) NAD(+)-isocitrate dehydrogenase subuni... 77 3e-13
>pir||B75160 3-isopropylmalate dehydrogenase (leub) PAB0289 - Pyrococcus abyssi
(strain Orsay) >gi|5457871|emb|CAB49361.1| (AJ248284)
3-isopropylmalate dehydrogenase (leuB) [Pyrococcus
abyssi]
Length = 346
Score = 691 bits (1763), Expect = 0.0
Identities = 346/346 (100%), Positives = 346/346 (100%)
Query: 1 MYRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRK 60
MYRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRK
Sbjct: 1 MYRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRK 60
Query: 61 MDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGKEIVIVRENTEGLYAR 120
MDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGKEIVIVRENTEGLYAR
Sbjct: 61 MDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGKEIVIVRENTEGLYAR 120
Query: 121 DGIGFSDRAIDFRIITLEGARRIAKFAINLAKERNSFITFVHKANVLKGDRFFREIVLEI 180
DGIGFSDRAIDFRIITLEGARRIAKFAINLAKERNSFITFVHKANVLKGDRFFREIVLEI
Sbjct: 121 DGIGFSDRAIDFRIITLEGARRIAKFAINLAKERNSFITFVHKANVLKGDRFFREIVLEI 180
Query: 181 AEREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGSIGIVPSGNYG 240
AEREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGSIGIVPSGNYG
Sbjct: 181 AEREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGSIGIVPSGNYG 240
Query: 241 DEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLNGKVVWEATRRYVRYGNLTP 300
DEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLNGKVVWEATRRYVRYGNLTP
Sbjct: 241 DEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLNGKVVWEATRRYVRYGNLTP 300
Query: 301 DMGGTATTSEVTKGIISEIYSFDPFDIDEIWVEEIRLGRIPLTLWR 346
DMGGTATTSEVTKGIISEIYSFDPFDIDEIWVEEIRLGRIPLTLWR
Sbjct: 301 DMGGTATTSEVTKGIISEIYSFDPFDIDEIWVEEIRLGRIPLTLWR 346
>pir||E71180 probable 3-isopropylmalate dehydrogenase - Pyrococcus horikoshii
>gi|3258153|dbj|BAA30836.1| (AP000007) 323aa long
hypothetical 3-isopropylmalate dehydrogenase [Pyrococcus
horikoshii]
Length = 323
Score = 489 bits (1246), Expect = e-137
Identities = 236/322 (73%), Positives = 283/322 (87%)
Query: 23 VVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKMDAILFGATTTPFNVPGYRSLI 82
+V SVTD+I+FYEFEGG VFK+ G PI E+DL+EIRKMDAILFGATTTPF+VP Y+SLI
Sbjct: 1 MVKSVTDKIKFYEFEGGLSVFKKYGVPIREEDLEEIRKMDAILFGATTTPFDVPRYKSLI 60
Query: 83 VTLRKELDLYANLRIIPDLSNGKEIVIVRENTEGLYARDGIGFSDRAIDFRIITLEGARR 142
+TLRKELDLYANLRIIP+ KEI+IVREN+EGLY+ +G S++ +DFRIIT +GA R
Sbjct: 61 ITLRKELDLYANLRIIPNFKLRKEIIIVRENSEGLYSGEGAYDSNKVVDFRIITRKGAER 120
Query: 143 IAKFAINLAKERNSFITFVHKANVLKGDRFFREIVLEIAEREGVEVREAIIDSFMIKLVK 202
IAKFA+ LAK+R++F+TFVHKAN+L+ DRFFR+IVL+IA +E V+VRE I+DSF IKLVK
Sbjct: 121 IAKFAVKLAKDRSTFLTFVHKANILESDRFFRKIVLDIARKEDVKVREEIVDSFTIKLVK 180
Query: 203 NPWDHGVILTENMFGDIISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIA 262
+PW+ G+IL+ENMFGDI+SDLATIHAGSIGIVPSGNYG++IALFEPIHGSAPDIAGKGIA
Sbjct: 181 DPWNLGIILSENMFGDILSDLATIHAGSIGIVPSGNYGEDIALFEPIHGSAPDIAGKGIA 240
Query: 263 NPIGAILSAAMMLDYLGLNGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEIYSF 322
NPIGAILSAAMMLDYLGL+G ++W+A RYVR GNLTPDM G ATT EVT GIISEIY
Sbjct: 241 NPIGAILSAAMMLDYLGLDGSIIWKAVGRYVRRGNLTPDMEGRATTLEVTNGIISEIYRL 300
Query: 323 DPFDIDEIWVEEIRLGRIPLTL 344
D ++IDE+W +E+RLGRI L +
Sbjct: 301 DEYEIDEVWRDEVRLGRILLEI 322
>pir||A69113 isocitrate dehydrogenase - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2621228|gb|AAB84690.1| (AE000806)
isocitrate dehydrogenase [Methanobacterium
thermoautotrophicum]
Length = 331
Score = 247 bits (625), Expect = 1e-64
Identities = 147/330 (44%), Positives = 203/330 (60%), Gaps = 14/330 (4%)
Query: 1 MYRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRK 60
MYR+ VI GDGIG EV+++A+ V+ ++ F E G E F+R G + E+ LK +RK
Sbjct: 1 MYRITVIPGDGIGVEVMEAALHVLQALEIEFEFTHAEAGNECFRRCGDTLPEETLKLVRK 60
Query: 61 MDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRII------PDLSNGKEIVIVRENT 114
DA LFGA TT VPG +S I+TLR+ELDL+ANLR + P L + VIVRENT
Sbjct: 61 ADATLFGAVTT---VPGQKSAIITLRRELDLFANLRPVKSLPGVPCLYPDLDFVIVRENT 117
Query: 115 EGLYARDGIGFSDRAIDFRIITLEGARRIAKFAINLA-KERNSFITFVHKANVLKG-DRF 172
E LY D + A+ RIIT +RRI++FA A KE +T VHKANVLK D
Sbjct: 118 EDLYVGDEEYTPEGAVAKRIITRTASRRISQFAFQYAQKEGMQKVTAVHKANVLKKTDGI 177
Query: 173 FREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGSI 231
FR+ ++A E +E + +D+ + L+ P + I+T N+FGDI+SD A G +
Sbjct: 178 FRDEFYKVASEYPQMEANDYYVDATAMYLITQPQEFQTIVTTNLFGDILSDEAAGLIGGL 237
Query: 232 GIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLG--LNGKVVWEAT 289
G+ PS N G++ ALFEP+HGSAP IAGK IANP IL+ +ML +L + + +A
Sbjct: 238 GLAPSANIGEKNALFEPVHGSAPQIAGKNIANPTAMILTTTLMLKHLNKKQEAQKIEKAL 297
Query: 290 RRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
++ + G +TPD+GGTA+T E+ + I EI
Sbjct: 298 QKTLMRGIMTPDLGGTASTMEMAEAIKEEI 327
>sp|Q58991|IDH_METJA ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCCINATE DECARBOXYLASE)
(IDH) (NADP+-SPECIFIC ICDH) (IDP)
>gi|2826436|gb|AAB99614.1| (U67599) 3-isopropylmalate
dehydrogenase 2 (leuB2) [Methanococcus jannaschii]
Length = 347
Score = 245 bits (618), Expect = 7e-64
Identities = 143/341 (41%), Positives = 206/341 (59%), Gaps = 23/341 (6%)
Query: 1 MYRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRK 60
M +V VI+GDGIG EV+ AI+++N + + + E G E K+ G+ + ED +++ ++
Sbjct: 1 MMKVCVIEGDGIGKEVIPEAIKILNELGE-FEIIKGEAGLECLKKYGNALPEDTIEKAKE 59
Query: 61 MDAILFGATTTPF--NVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------------- 105
D ILFGA T+P V Y+S I+TLRK LYAN+R I + G+
Sbjct: 60 ADIILFGAITSPKPGEVQNYKSPIITLRKMFHLYANVRPINNFGIGQLIGKIADYEFLNA 119
Query: 106 ---EIVIVRENTEGLYARDGIGFSDRAIDFRIITLEGARRIAKFAINLA-KERNSFITFV 161
+IVI+RENTE LY +D AI R+IT +G+ RI +FA A K ++ +
Sbjct: 120 KNIDIVIIRENTEDLYVGRERLENDTAIAERVITRKGSERIIRFAFEYAIKNNRKKVSCI 179
Query: 162 HKANVLK-GDRFFREIVLEIAEREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDII 220
HKANVL+ D F E+ EI + +E + ++DS + L+K+P VI+T NMFGDI+
Sbjct: 180 HKANVLRITDGLFLEVFNEIKKHYNIEADDYLVDSTAMNLIKHPEKFDVIVTTNMFGDIL 239
Query: 221 SDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLG- 279
SD A+ G +G+ PS N GD+ ALFEP+HGSAPDIAGKGIANP+ +ILS AM+ DY+G
Sbjct: 240 SDEASALIGGLGLAPSANIGDDKALFEPVHGSAPDIAGKGIANPMASILSIAMLFDYIGE 299
Query: 280 -LNGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
G ++ EA + + +TPD+GG T +V I++ I
Sbjct: 300 KEKGDLIREAVKYCLINKKVTPDLGGDLKTKDVGDEILNYI 340
>pir||C64499 isocitrate dehydrogenase (EC 1.1.1.-) - Methanococcus jannaschii
Length = 371
Score = 243 bits (613), Expect = 3e-63
Identities = 142/341 (41%), Positives = 205/341 (59%), Gaps = 23/341 (6%)
Query: 1 MYRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRK 60
M +V VI+GDGIG EV+ A +++N + + + E G E K+ G+ + ED +++ ++
Sbjct: 25 MMKVCVIEGDGIGKEVIPEAXKILNELGE-FEIIKGEAGLECLKKYGNALPEDTIEKAKE 83
Query: 61 MDAILFGATTTPF--NVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------------- 105
D ILFGA T+P V Y+S I+TLRK LYAN+R I + G+
Sbjct: 84 ADIILFGAITSPKPGEVQNYKSPIITLRKMFHLYANVRPINNFGIGQLIGKIADYEFLNA 143
Query: 106 ---EIVIVRENTEGLYARDGIGFSDRAIDFRIITLEGARRIAKFAINLA-KERNSFITFV 161
+IVI+RENTE LY +D AI R+IT +G+ RI +FA A K ++ +
Sbjct: 144 KNIDIVIIRENTEDLYVGRERLENDTAIAERVITRKGSERIIRFAFEYAIKNNRKKVSCI 203
Query: 162 HKANVLK-GDRFFREIVLEIAEREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDII 220
HKANVL+ D F E+ EI + +E + ++DS + L+K+P VI+T NMFGDI+
Sbjct: 204 HKANVLRITDGLFLEVFNEIKKHYNIEADDYLVDSTAMNLIKHPEKFDVIVTTNMFGDIL 263
Query: 221 SDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLG- 279
SD A+ G +G+ PS N GD+ ALFEP+HGSAPDIAGKGIANP+ +ILS AM+ DY+G
Sbjct: 264 SDEASALIGGLGLAPSANIGDDKALFEPVHGSAPDIAGKGIANPMASILSIAMLFDYIGE 323
Query: 280 -LNGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
G ++ EA + + +TPD+GG T +V I++ I
Sbjct: 324 KEKGDLIREAVKYCLINKKVTPDLGGDLKTKDVGDEILNYI 364
>pir||E75368 probable isocitrate dehydrogenase - Deinococcus radiodurans
(strain R1) >gi|6459438|gb|AAF11228.1|AE002009_8
(AE002009) isocitrate dehydrogenase, putative
[Deinococcus radiodurans]
Length = 333
Score = 224 bits (564), Expect = 2e-57
Identities = 131/329 (39%), Positives = 189/329 (56%), Gaps = 11/329 (3%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKM 61
YR+ +I+GDGIG EV+ +A RV+ + + E G+E F G+ + E +
Sbjct: 4 YRICLIEGDGIGHEVIPAAKRVLEAAGFDAEYVHAEAGYEYFLDHGTSVPEATYDAVENT 63
Query: 62 DAILFGATTTPFNVP--GYRSLIVTLRKELDLYANLRI-----IPDLSNGKEIVIVRENT 114
DA LFGA T+P G+ I LR++ +LYAN+R +P ++VIVRENT
Sbjct: 64 DATLFGAATSPSGEKPAGFFGAIRHLRQKYNLYANVRPTKTRPVPHSYENVDLVIVRENT 123
Query: 115 EGLYARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKERNSFITFVHKANVLKGDR--F 172
+GLY + D AI +IT E + RI KFA +LA +R+ +T VHK+NVL + F
Sbjct: 124 QGLYVEQERRYGDTAIADTVITREASDRIGKFAADLAMKRSKRLTVVHKSNVLPVTQGLF 183
Query: 173 FREIVLEIAEREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGSIG 232
I+ EG+ I+D+ ++LV+NP V++ NMFGDI+SDLA G +G
Sbjct: 184 MNTILDHTKTVEGLSTSTMIVDNAAMQLVRNPQQFDVMVMTNMFGDILSDLAAGLVGGLG 243
Query: 233 IVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLG--LNGKVVWEATR 290
I SGN GD+ +FE +HGSAPDIAG+GI+NP IL+A +MLD+LG + + A
Sbjct: 244 IAASGNVGDQFGIFESVHGSAPDIAGQGISNPTATILAAVIMLDHLGDHETARRLDNAIN 303
Query: 291 RYVRYGNLTPDMGGTATTSEVTKGIISEI 319
+ + T D+GGTA T E T+ +I +
Sbjct: 304 KVLAEXPRTRDLGGTAGTQEFTEAVIKAL 332
>gi|11498236 3-isopropylmalate dehydrogenase (leuB) [Archaeoglobus fulgidus]
>gi|6685585|sp|O29627|LEU3_ARCFU 3-ISOPROPYLMALATE
DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH) (3-IPM-DH)
>gi|7431327|pir||D69328 3-isopropylmalate dehydrogenase
(leuB) homolog - Archaeoglobus fulgidus
>gi|2649991|gb|AAB90611.1| (AE001061) 3-isopropylmalate
dehydrogenase (leuB) [Archaeoglobus fulgidus]
Length = 326
Score = 220 bits (555), Expect = 2e-56
Identities = 132/328 (40%), Positives = 190/328 (57%), Gaps = 16/328 (4%)
Query: 1 MYRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRK 60
M ++ VI GDGIG EV+++A+ ++ + + ++ G E ++ G + ++ L+ RK
Sbjct: 1 MKKIVVIPGDGIGKEVMEAAMLILEKLDLPFEYSYYDAGDEALEKYGKALPDETLEACRK 60
Query: 61 MDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRI------IPDLSNGKEIVIVRENT 114
DA+LFGA +IV LR+EL +AN+R I L G +IV+VRENT
Sbjct: 61 SDAVLFGAAGETA-----ADVIVRLRRELGTFANVRPAKAIEGIECLYPGLDIVVVRENT 115
Query: 115 EGLYARDGIGFSDRAIDFRIITLEGARRIAKFAINLAK-ERNSFITFVHKANVLKGD-RF 172
E LY GF D R+IT E + RIA++A LAK E +T +HKANV+K
Sbjct: 116 ECLYMGFEFGFGDVTEAIRVITREASERIARYAFELAKREGRKKVTALHKANVMKKTCGL 175
Query: 173 FREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGSI 231
FR++ E+A + ++ + ID+ + LV +P+ VI+T NMFGDI+SDLA G +
Sbjct: 176 FRDVCREVAKDYPEIQYNDYYIDAACMYLVMDPFRFDVIVTTNMFGDIVSDLAAGLVGGL 235
Query: 232 GIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVVWEAT 289
G+ PS N G+ A+FEP+HG+A DIAGKGIANP IL+A MML + G K V EA
Sbjct: 236 GLAPSANVGERTAIFEPVHGAAFDIAGKGIANPTAMILTACMMLRHFGYVEEAKKVEEAV 295
Query: 290 RRYVRYGNLTPDMGGTATTSEVTKGIIS 317
+ ++ G TPD+GG T E + S
Sbjct: 296 EKTIKEGKKTPDLGGNLKTMEFANEVAS 323
>sp|Q58130|LEU3_METJA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|2826313|gb|AAB98716.1| (U67519)
3-isopropylmalate dehydrogenase 1 (leuB1) [Methanococcus
jannaschii]
Length = 333
Score = 214 bits (539), Expect = 1e-54
Identities = 134/335 (40%), Positives = 185/335 (55%), Gaps = 21/335 (6%)
Query: 1 MYRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRK 60
M+++ VI+GDGIG EVV + I+V+ + F E G EV+KR G + E+ ++
Sbjct: 1 MHKICVIEGDGIGKEVVPATIQVLEATGLPFEFVYAEAGDEVYKRTGKALPEETIETALD 60
Query: 61 MDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRENT 114
DA+LFGA +IV LR LD YAN+R + K + VIVRENT
Sbjct: 61 CDAVLFGAAGETA-----ADVIVKLRHILDTYANIRPVKAYKGVKCLRPDIDYVIVRENT 115
Query: 115 EGLYARDGIGFSDR-AIDFRIITLEGARRIAKFAINLAKERNSF-----ITFVHKANVLK 168
EGLY + I R+IT + RI +FA NLA+ER +T HKANVLK
Sbjct: 116 EGLYKGIEAEIDEGITIATRVITEKACERIFRFAFNLARERKKMGKEGKVTCAHKANVLK 175
Query: 169 -GDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATI 226
D F++I ++AE + ++ + ID+ + ++ P V++T N+FGDI+SD A
Sbjct: 176 LTDGLFKKIFYKVAEEYDDIKAEDYYIDAMNMYIITKPQVFDVVVTSNLFGDILSDGAAG 235
Query: 227 HAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLG--LNGKV 284
G +G+ PS N GDE LFEP+HGSAPDIAGK IANP ILSA +ML YLG
Sbjct: 236 TVGGLGLAPSANIGDEHGLFEPVHGSAPDIAGKKIANPTATILSAVLMLRYLGEYEAADK 295
Query: 285 VWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
V +A + G TPD+GG T E+ + + +
Sbjct: 296 VEKALEEVLALGLTTPDLGGNLNTFEMAEEVAKRV 330
>pir||H64389 isocitrate dehydrogenase (NADP+) (EC 1.1.1.42) - Methanococcus
jannaschii
Length = 337
Score = 214 bits (539), Expect = 1e-54
Identities = 134/335 (40%), Positives = 185/335 (55%), Gaps = 21/335 (6%)
Query: 1 MYRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRK 60
M+++ VI+GDGIG EVV + I+V+ + F E G EV+KR G + E+ ++
Sbjct: 5 MHKICVIEGDGIGKEVVPATIQVLEATGLPFEFVYAEAGDEVYKRTGKALPEETIETALD 64
Query: 61 MDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRENT 114
DA+LFGA +IV LR LD YAN+R + K + VIVRENT
Sbjct: 65 CDAVLFGAAGETA-----ADVIVKLRHILDTYANIRPVKAYKGVKCLRPDIDYVIVRENT 119
Query: 115 EGLYARDGIGFSDR-AIDFRIITLEGARRIAKFAINLAKERNSF-----ITFVHKANVLK 168
EGLY + I R+IT + RI +FA NLA+ER +T HKANVLK
Sbjct: 120 EGLYKGIEAEIDEGITIATRVITEKACERIFRFAFNLARERKKMGKEGKVTCAHKANVLK 179
Query: 169 -GDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATI 226
D F++I ++AE + ++ + ID+ + ++ P V++T N+FGDI+SD A
Sbjct: 180 LTDGLFKKIFYKVAEEYDDIKAEDYYIDAMNMYIITKPQVFDVVVTSNLFGDILSDGAAG 239
Query: 227 HAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLG--LNGKV 284
G +G+ PS N GDE LFEP+HGSAPDIAGK IANP ILSA +ML YLG
Sbjct: 240 TVGGLGLAPSANIGDEHGLFEPVHGSAPDIAGKKIANPTATILSAVLMLRYLGEYEAADK 299
Query: 285 VWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
V +A + G TPD+GG T E+ + + +
Sbjct: 300 VEKALEEVLALGLTTPDLGGNLNTFEMAEEVAKRV 334
>pir||D75045 3-isopropylmalate dehydrogenase (leub) PAB2424 - Pyrococcus abyssi
(strain Orsay) >gi|5458770|emb|CAB50257.1| (AJ248287)
3-isopropylmalate dehydrogenase (leuB) [Pyrococcus
abyssi]
Length = 354
Score = 213 bits (537), Expect = 2e-54
Identities = 136/340 (40%), Positives = 191/340 (56%), Gaps = 27/340 (7%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIR----FYEFEGGFEVFKRIGSPISEDDLKEI 58
R+AVI GDGIG EVV ++V+ + + R F E+ G E + + G + + ++E
Sbjct: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
Query: 59 RKMDAILFGATTTPFNVPGY--RSLIVTLRKELDLYANLRII----PDLS-----NGKEI 107
+K DAI FGA P PG R +++ +R ELDLY NLR + P L+ N +I
Sbjct: 64 KKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDI 123
Query: 108 VIVRENTEGLYARDGIGF------SDRAIDFRIITLEGARRIAKFAINLAKERN-SFITF 160
V VRENTEGLYA G GF + A+ I T G R+ +FA AK +T
Sbjct: 124 VFVRENTEGLYAGAG-GFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTL 182
Query: 161 VHKANVLK-GDRFFREIVLEIAEREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDI 219
V KANVL + + E+++ +E +D+ +K++++P V++T NMFGDI
Sbjct: 183 VDKANVLTYAHDLWERVFAEVSQEYDLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDI 242
Query: 220 ISDLATIHAGSIGIVPSGNYGDEIA-LFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYL 278
++DL G +GI SGN E +FEP+HGSAPDIAGK IANP+ AILSA+MML+YL
Sbjct: 243 LTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYL 302
Query: 279 GLNGKVVW--EATRRYVRYGNLTPDMGGTATTSEVTKGII 316
L + W EA +R + +TPDM G T EV ++
Sbjct: 303 NLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVV 342
>gi|6322097 Homo-isocitrate dehydrogenase; Lys12p [Saccharomyces cerevisiae]
>gi|731845|sp|P40495|YIJ4_YEAST HYPOTHETICAL 40.1 KD
PROTEIN IN SGA1-KTR7 INTERGENIC REGION
>gi|1076985|pir||S49786 3-isopropylmalate dehydrogenase
homolog YIL094c - yeast (Saccharomyces cerevisiae)
>gi|577120|emb|CAA86700.1| (Z46728) YI9910.02c, orf,
len: 371, CAI: 0.31, similar to isocitrate and
isopropylmalate dehyrogenases [Saccharomyces cerevisiae]
Length = 371
Score = 213 bits (537), Expect = 2e-54
Identities = 131/346 (37%), Positives = 202/346 (57%), Gaps = 30/346 (8%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKEIR 59
+ +I GDGIG EV+ + +V+ ++ + F + GF+ F+ G + ++ +K ++
Sbjct: 26 IGLIPGDGIGKEVIPAGKQVLENLNSKHGLSFNFIDLYAGFQTFQETGKALPDETVKVLK 85
Query: 60 KM-DAILFGATTTPFN-VPGYRSLIVTLRKELDLYANLRIIPDLSNGK----EIVIVREN 113
+ LFGA +P V GY S IV LR+E+ L+AN+R + + K ++VIVREN
Sbjct: 86 EQCQGALFGAVQSPTTKVEGYSSPIVALRREMGLFANVRPVKSVEGEKGKPIDMVIVREN 145
Query: 114 TEGLYARDGIGFSDRAIDFRI------ITLEGARRIAKFAINLAKER-----NSFITFVH 162
TE LY + + D+A R+ I+ RRIA A+++A +R + +T H
Sbjct: 146 TEDLYIKIEKTYIDKATGTRVADATKRISEIATRRIATIALDIALKRLQTRGQATLTVTH 205
Query: 163 KANVL-KGDRFFREIVLEIAEREG-----VEVREAIIDSFMIKLVKNPWDHGVILTENMF 216
K+NVL + D FREI E+ E ++ E I+DS + +L + P VI+ N++
Sbjct: 206 KSNVLSQSDGLFREICKEVYESNKDKYGQIKYNEQIVDSMVYRLFREPQCFDVIVAPNLY 265
Query: 217 GDIISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLD 276
GDI+SD A GS+G+VPS N G EI + EP HGSAPDIAGKGIANPI I S A+ML+
Sbjct: 266 GDILSDGAAALVGSLGVVPSANVGPEIVIGEPCHGSAPDIAGKGIANPIATIRSTALMLE 325
Query: 277 YLGLN--GKVVWEATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
+LG N + +++A +R G++ TPD+GG A+T +V ++S +
Sbjct: 326 FLGHNEAAQDIYKAVDANLREGSIKTPDLGGKASTQQVVDDVLSRL 371
>sp|O66607|LEU3_AQUAE 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|7431317|pir||C70322 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Aquifex aeolicus
>gi|2982949|gb|AAC06564.1| (AE000680) 3-isopropylmalate
dehydrogenase [Aquifex aeolicus]
Length = 364
Score = 210 bits (530), Expect = 2e-53
Identities = 135/349 (38%), Positives = 199/349 (56%), Gaps = 31/349 (8%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTD--RIRFYEFEG--GFEVFKRIGSPISEDDLKE 57
Y++AV+KGDGIGPE+V+ A+RV++ + + + F EG G E + G P+ E+ LK
Sbjct: 4 YKIAVLKGDGIGPEIVEQALRVLDKIGEIYGVEFEYREGLIGGEAIDKTGDPLPEETLKI 63
Query: 58 IRKMDAILFGATTTPF--NVPGYRSL---IVTLRKELDLYANLR-------------IIP 99
++ DAIL GA P N+P + ++ +RKELDLYANLR +
Sbjct: 64 CKESDAILLGAVGGPKWDNLPTDKRPEKGLLRIRKELDLYANLRPAKVWDALISSSPLKE 123
Query: 100 DLSNGKEIVIVRENTEGLYARDGIGFSDR-----AIDFRIITLEGARRIAKFAINLAKER 154
++ G +++++RE T G+Y + G + AI+ T + RRI + +A++R
Sbjct: 124 EVVKGTDMIVIRELTSGIYYGEPRGIFEENGKRYAINTMKYTEDEIRRIVRKGFEIARKR 183
Query: 155 NSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTE 213
+T V KANVL+ +R+IV E E VE+ ID+ ++LV+ P VI+T
Sbjct: 184 RKKLTSVDKANVLEVSALWRQIVEEEKENYPDVELEHLYIDNCAMQLVRRPTSFDVIVTG 243
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAM 273
N+FGDI+SD A + GS+G++PS + GD AL+EP+HGSAPDIAGKGIANPI ILSAAM
Sbjct: 244 NIFGDILSDEAGVVVGSLGMLPSASIGDRYALYEPVHGSAPDIAGKGIANPIATILSAAM 303
Query: 274 MLDY---LGLNGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
ML Y + ++ +A + G TPD+ TK I +I
Sbjct: 304 MLKYSFNMDKAHDLIEKAIEETLNQGYRTPDIYSEGCIKVGTKEITDKI 352
>dbj|BAB06776.1| (AP001517) 3-isopropylmalate dehydrogenase [Bacillus halodurans]
Length = 363
Score = 204 bits (514), Expect = 1e-51
Identities = 133/350 (38%), Positives = 198/350 (56%), Gaps = 34/350 (9%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRI-RFYEFE----GGFEVFKRIGSPISEDDLKE 57
++AV+ GDGIGPEV D+AI V+ +V DR + ++ GG + +G+P+ E+ L
Sbjct: 4 QIAVLPGDGIGPEVTDAAIEVLQAVADRFGHTFSYKKALLGGCAI-DEVGTPLPEETLDV 62
Query: 58 IRKMDAILFGATTTPF--NVPGY---RSLIVTLRKELDLYANLRIIP------------- 99
R D IL GA P +PG+ ++ LRK L+L+ANLR +
Sbjct: 63 CRHADGILLGAVGGPKWDTLPGHLRPEKGLLGLRKGLNLFANLRPVTVYDSLADASTLKN 122
Query: 100 DLSNGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKER 154
D+ +G +++IVRE T GLY R G G ++ +D + T RRI + A LA R
Sbjct: 123 DVIDGVDLLIVRELTGGLYFGEPRERRGEGETEEVVDTLLYTRGEMRRIIRKAFELAMVR 182
Query: 155 NSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTE 213
N +T V KANVL+ R +RE+ E+A+ V + ++D+ ++L++ P V++TE
Sbjct: 183 NKHVTSVDKANVLESSRMWREVANEVAQEFPEVTLEHMLVDNAAMQLIRRPKQFDVLVTE 242
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNY-GDEIALFEPIHGSAPDIAGKGIANPIGAILSAA 272
N+FGDI+SD A++ GS+G++PS + D L+EPIHGSAPDIAGKG+ANP+ I S A
Sbjct: 243 NLFGDILSDEASMVTGSLGMLPSASLTSDGPGLYEPIHGSAPDIAGKGVANPLATIASCA 302
Query: 273 MMLDY-LGLN--GKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
MML Y GL+ K + +A ++ G T D+ S TK I +
Sbjct: 303 MMLKYSFGLHEEAKTIEDAIEAVLKQGYRTADIAKPGEESSSTKAITDAV 352
>sp|O27441|LEU3_METTH 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|7431326|pir||F69051 3-isopropylmalate
dehydrogenase - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2622498|gb|AAB85865.1| (AE000901)
3-isopropylmalate dehydrogenase [Methanobacterium
thermoautotrophicum]
Length = 329
Score = 204 bits (514), Expect = 1e-51
Identities = 128/324 (39%), Positives = 187/324 (57%), Gaps = 16/324 (4%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKMD 62
++AVI GDGIG EV+++A+ ++N++ + F + G KR G+ + E+ L+ + +
Sbjct: 5 KIAVIPGDGIGVEVMEAALHILNTLDLDLEFIHADAGDACLKRTGTALPEETLEAVGEAR 64
Query: 63 AILFGATTTPFNVPGYRSLIVTLRKELDLYANLRII------PDLSNGKEIVIVRENTEG 116
A LFGA +IV LR+E DL+ANLR + P L + VIVRENTE
Sbjct: 65 ATLFGAAGE-----SAADVIVRLRREFDLFANLRPVKSLPGVPCLYPDLDFVIVRENTED 119
Query: 117 LYARDGIGFSDRAIDFRIITLEGARRIAKFAINLA-KERNSFITFVHKANVLKG-DRFFR 174
LY D + A+ RIIT +RRI++FA A KE +T VHKANVLK D FR
Sbjct: 120 LYVGDEEYTPEGAVAKRIITRTASRRISQFAFQYAQKEGMQKVTAVHKANVLKKTDGIFR 179
Query: 175 EIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGSIGI 233
+ ++A E +E + +D+ + L+ P + I+T N+FGDI+SD A G +G+
Sbjct: 180 DEFYKVASEYPQMEATDYYVDATAMYLITQPQEFQTIVTTNLFGDILSDEAAGLIGGLGL 239
Query: 234 VPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLG--LNGKVVWEATRR 291
PS N G++ ALFEP+HGSAP IAGK IANP IL+ +ML +L + + +A ++
Sbjct: 240 APSANIGEKNALFEPVHGSAPQIAGKNIANPTAMILTTTLMLKHLNKKQEAQKIEKALQK 299
Query: 292 YVRYGNLTPDMGGTATTSEVTKGI 315
+ G +TPD+GG T E+ I
Sbjct: 300 TLAEGLVTPDLGGKLGTMEMAAEI 323
>sp|Q02143|LEU3_LACLA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|486725|pir||S35133 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Lactococcus lactis subsp.
lactis >gi|2565152|gb|AAB81914.1| (U92974) LeuB
[Lactococcus lactis]
Length = 345
Score = 202 bits (508), Expect = 6e-51
Identities = 135/339 (39%), Positives = 197/339 (57%), Gaps = 26/339 (7%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRF-YEFE----GGFEVFKRIGSPISEDDLKE 57
++ + GDGIGPE++ + + V+ +V+ +I F YE E GG + K G P+ E+ L+
Sbjct: 4 KIVTLAGDGIGPEIMSAGLSVLKAVSKKIDFEYELEAKDFGGIAIDKH-GHPLPEETLQA 62
Query: 58 IRKMDAILFGATTTP-FNVPGYRSL--IVTLRKELDLYANLR---IIPDLSN-------- 103
++ DAIL A P +N R ++ LRKEL LYAN+R I P L
Sbjct: 63 VKNADAILLAAIGHPKYNNAKVRPEQGLLALRKELGLYANVRPLKIYPALKKLSPIRNVE 122
Query: 104 GKEIVIVRENTEGLYARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKER-NSFITFVH 162
+ +++RE T G+Y D+A D + + RRI FA A+ R +T V
Sbjct: 123 NVDFLVIRELTGGIYFGQHELADDKARDVNDYSADEIRRILHFAFKSAQSRPRKLLTSVD 182
Query: 163 KANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIIS 221
K NVL + +R++ EIA E V + ++DS + L+ NP VI+TEN+FGDI+S
Sbjct: 183 KQNVLATSKLWRKMADEIADEYPDVRLEHQLVDSCAMLLITNPQQFDVIVTENLFGDILS 242
Query: 222 DLATIHAGSIGIVPSGNYG-DEIALFEPIHGSAPDIAGKGIANPIGAILSAAMML-DYLG 279
D A+ AGS+G++PS ++G + +AL+EPIHGSAPDIAGKGIANP+ ILS AMML + G
Sbjct: 243 DEASSLAGSLGVMPSSSHGFNGLALYEPIHGSAPDIAGKGIANPVSMILSIAMMLRESFG 302
Query: 280 L--NGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGII 316
++ +A + G LT D+GGTATT E+T+ I+
Sbjct: 303 QEDGAAMIEKAVTQTFTDGILTKDLGGTATTKEMTEAIL 341
>sp|P24015|LEU3_LEPIN 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|149619|gb|AAA72088.1| (M59431)
3-isopropylmalate dehydrogenase [Leptospira
borgpetersenii]
Length = 358
Score = 201 bits (505), Expect = 1e-50
Identities = 135/357 (37%), Positives = 196/357 (54%), Gaps = 35/357 (9%)
Query: 1 MYRVAVIKGDGIGPEVVDSAIRVVNSVTD-RIRFYEFEGGFE---VFKRIGSPISEDDLK 56
M VAV+ GDGIGPEV++ AI V+ ++ ++F+ GF + G P+ + LK
Sbjct: 1 MKNVAVLSGDGIGPEVMEIAISVLKKALGAKVSEFQFKEGFVGGIAIDKTGHPLPPETLK 60
Query: 57 EIRKMDAILFGAT------TTPFNVPGYRSLIVTLRKELDLYANLR---IIPDLSN---- 103
+ AILFG+ T P R ++ LRK DL+ANLR I P+L N
Sbjct: 61 LCEESSAILFGSVGGPKWETLPPEKQPERGALLPLRKHFDLFANLRPAIIYPELKNASPV 120
Query: 104 -------GKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLA 151
G +I+I+RE T G+Y R+G G + A D + RIAK A A
Sbjct: 121 RSDIIGNGLDILILRELTGGIYFGQPKGREGSGQEEFAYDTMKYSRREIERIAKVAFQAA 180
Query: 152 KERNSFITFVHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWDHGV 209
++RN+ +T + KANVL F++E+V+E+ ++E V++ +D+ ++L+ NP V
Sbjct: 181 RKRNNKVTSIDKANVLTTSVFWKEVVIELHKKEFSDVQLNHLYVDNAAMQLIVNPKQFDV 240
Query: 210 ILTENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAI 268
+L ENMFGDI+SD A+I GSIG++PS + + L+EP GSAPDIAGKG+ANPI +
Sbjct: 241 VLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGVANPIAQV 300
Query: 269 LSAAMMLDY---LGLNGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEIYSF 322
LSAA+ML Y + + A R+ + G T D+ +T TK I I SF
Sbjct: 301 LSAALMLRYSFSMEEEANKIETAVRKTIASGKRTRDIAEVGSTIVGTKEIGQLIESF 357
>pir||A82539 isocitrate dehydrogenase XF2596 [imported] - Xylella fastidiosa
(strain 9a5c) >gi|9107816|gb|AAF85393.1|AE004066_7
(AE004066) isocitrate dehydrogenase [Xylella fastidiosa]
Length = 335
Score = 199 bits (500), Expect = 5e-50
Identities = 119/336 (35%), Positives = 183/336 (54%), Gaps = 25/336 (7%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKMDA 63
+ VI+GDGIGPE++D+ + V+N++ + + + G ++ G + E L I K
Sbjct: 5 ITVIRGDGIGPEIMDATLLVLNTLQANLNYEFADAGLNALEKHGDLLPEATLNSISKNKV 64
Query: 64 ILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK-------EIVIVRENTEG 116
L TTP G+ S+ V LR++ DLYAN+R N K +++ VRENTEG
Sbjct: 65 ALKSPLTTPVG-EGFSSINVALRRKFDLYANVRPAKSFPNTKSRFSDNVDLITVRENTEG 123
Query: 117 LYARDGIGFS---DRAIDFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLKGDR- 171
Y +G S + A +T +G+ RI ++A +LA+ +T VHKAN++K
Sbjct: 124 AYLSEGQTISPDGEVATSSARVTRKGSERIVRYAFDLARTTGRKKVTAVHKANIIKSTSG 183
Query: 172 FFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGS 230
F + ++A + ++ +E I+D+ ++LV P +I+T N+FGDI+SDL G
Sbjct: 184 LFLKTARDVASQYPEIDFQEMIVDNACMQLVMRPEQFDIIVTTNLFGDILSDLCAGLVGG 243
Query: 231 IGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLG-------LNGK 283
+G+ P N G+E A+FE +HGSAPDIAG+G ANP +L A MLD++G L
Sbjct: 244 LGLAPGANIGNEQAIFEAVHGSAPDIAGQGKANPCALLLGAVQMLDHIGQPQNAERLRNA 303
Query: 284 VVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
+V + +LTPD+GG T E K I S +
Sbjct: 304 IVATLNAK----DSLTPDLGGNGNTMEFAKAIASRL 335
>sp|P50455|LEU3_SULS7 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|1890070|dbj|BAA13178.1| (D86857)
3-isopropylmalate dehydrogenase [Sulfolobus sp. 7]
Length = 337
Score = 198 bits (499), Expect = 7e-50
Identities = 137/347 (39%), Positives = 190/347 (54%), Gaps = 42/347 (12%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
+ VA+I+GDGIGPE+V + R++ + + I + E E G R G + +D LK
Sbjct: 3 FTVALIQGDGIGPEIVSKSKRILAKINELYSLPIEYIEVEAGDRALARYGEALPKDSLKI 62
Query: 58 IRKMDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLR---IIPDLSN---GKEIVIVR 111
I K D IL G ++V LR+ D+YAN+R IP + +I+IVR
Sbjct: 63 IDKADIILKGPVGE-----SAADVVVKLRQIYDMYANIRPAKSIPGIDTKYGNVDILIVR 117
Query: 112 ENTEGLY------ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKERNSFITFVHKAN 165
ENTE LY DG+ A+ +IIT + RIAK +N A R +T VHKAN
Sbjct: 118 ENTEDLYKGFEHIVSDGV-----AVGMKIITRFASERIAKVGLNFALRRRKKVTCVHKAN 172
Query: 166 VLK-GDRFFREIVLEIAEREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLA 224
V++ D F E + + + VE E +D+ LV+NP VI+TEN++GDI+SD A
Sbjct: 173 VMRITDGLFAEACRSVLKGK-VEYSEMYVDAAAANLVRNPQMFDVIVTENVYGDILSDEA 231
Query: 225 TIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLD--YLGLNG 282
+ AGS+GI PS N GD+ ALFEP+HG+A DIAGK I NP +LS +MM + Y N
Sbjct: 232 SQIAGSLGIAPSANIGDKKALFEPVHGAAFDIAGKNIGNPTAFLLSVSMMYERMYELSND 291
Query: 283 KVVWEATRR--------YVRYGNLTPDMGGTATTSEVTKGIISEIYS 321
+A+R Y LTPD+GG ATT + +I+EIY+
Sbjct: 292 DRYIKASRALENAIYLVYKERKALTPDVGGNATTDD----LINEIYN 334
>pir||T38621 probable isopropyl malate dehydrogenase - fission yeast
(Schizosaccharomyces pombe) >gi|2388955|emb|CAB11688.1|
(Z98979) putative homoisocitrate dehydrogenase; lysine
biosynthesis [Schizosaccharomyces pombe]
Length = 362
Score = 198 bits (498), Expect = 9e-50
Identities = 124/354 (35%), Positives = 197/354 (55%), Gaps = 36/354 (10%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRIR----FYEFEGGFEVFKRIGSPISEDDLKEIR 59
+ +I DGIG EVV +A R++ ++ + + F + + G+ F+R G + E ++ ++
Sbjct: 9 LGLIPADGIGKEVVPAARRLMENLPAKHKLKFDFIDLDAGWGTFERTGKALPERTVERLK 68
Query: 60 -KMDAILFGATTTPFN-VPGYRSLIVTLRKELDLYANLRIIPDLSNGK----EIVIVREN 113
+ +A LFGA +P + V GY S IV LRK++ LYAN+R + L K ++VIVREN
Sbjct: 69 TECNAALFGAVQSPTHKVAGYSSPIVALRKKMGLYANVRPVKSLDGAKGKPVDLVIVREN 128
Query: 114 TEGLYARDGIGFSDR-----AIDFRIITLEGARRIAKFAINLAKERNSF----------- 157
TE LY ++ + A R I+ E + +I K A +AK R
Sbjct: 129 TECLYVKEERMVQNTPGKRVAEAIRRISEEASTKIGKMAFEIAKSRQKIRESGTYSIHKK 188
Query: 158 --ITFVHKANVLK-GDRFFREIVLEIAERE----GVEVREAIIDSFMIKLVKNPWDHGVI 210
+T +HK+NV+ D FRE + + V E I+DS + +L + P V+
Sbjct: 189 PLVTIIHKSNVMSVTDGLFRESCRHAQSLDPSYASINVDEQIVDSMVYRLFREPECFDVV 248
Query: 211 LTENMFGDIISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILS 270
+ N++GDI+SD A GS+G+VPS N GD + EP+HGSAPDIAG+GIANP+ S
Sbjct: 249 VAPNLYGDILSDGAASLIGSLGLVPSANVGDNFVMSEPVHGSAPDIAGRGIANPVATFRS 308
Query: 271 AAMMLDYLGLNGKV--VWEATRRYVRYGN-LTPDMGGTATTSEVTKGIISEIYS 321
A+ML+++G ++ A + + G LTPD+GG + T+E+T +++ I++
Sbjct: 309 VALMLEFMGHQDAAADIYTAVDKVLTEGKVLTPDLGGKSGTNEITDAVLANIHN 362
>sp|P12010|LEU3_BACCO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|65953|pir||DEBSIC 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Bacillus coagulans
>gi|3212662|pdb|2AYQ|B Chain B, 3-Isopropylmalate
Dehydrogenase From The Moderate Facultative Thermophile,
Bacillus Coagulans >gi|3212661|pdb|2AYQ|A Chain A,
3-Isopropylmalate Dehydrogenase From The Moderate
Facultative Thermophile, Bacillus Coagulans
>gi|225041|prf||1207195A dehydrogenase,isopropylmalate
[Bacillus coagulans]
Length = 366
Score = 192 bits (482), Expect = 7e-48
Identities = 135/353 (38%), Positives = 194/353 (54%), Gaps = 37/353 (10%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYE-FEG---GFEVFKRIGSPISEDDLKEI 58
++AV+ GDGIGPEV+D+AIRV+ +V D FE G G+P+ E+ L
Sbjct: 4 KLAVLPGDGIGPEVMDAAIRVLKTVLDNDGHEAVFENALIGGAAIDEAGTPLPEETLDIC 63
Query: 59 RKMDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLRIIPDLSN---------- 103
R+ DAIL GA P SL ++ LRKE+ L+ANLR + +
Sbjct: 64 RRSDAILLGAVGGPKWDHNPASLRPEKGLLGLRKEMGLFANLRPVKAYATLLNASPLKRE 123
Query: 104 ---GKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN 155
++VIVRE T GLY R G G + +D T E RI + A LA+ R
Sbjct: 124 RVENVDLVIVRELTGGLYFGRPSERRGPG-ENEVVDTLAYTREEIERIIEKAFQLAQIRR 182
Query: 156 SFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTEN 214
+ V KANVL+ R +REI E A++ VE+ ++DS ++L+ NP VI+TEN
Sbjct: 183 KKLASVDKANVLESSRMWREIAEETAKKYPDVELSHMLVDSTSMQLIANPGQFDVIVTEN 242
Query: 215 MFGDIISDLATIHAGSIGIVPSGNY-GDEIALFEPIHGSAPDIAGKGIANPIGAILSAAM 273
MFGDI+SD A++ GS+G++PS + D ++EP+HGSAPDIAG+G ANP+G +LSAA+
Sbjct: 243 MFGDILSDEASVITGSLGMLPSASLRSDRFGMYEPVHGSAPDIAGQGKANPLGTVLSAAL 302
Query: 274 MLDY---LGLNGKVVWEATRRYVRYGNLTPDM----GGTATTSEVTKGIISEI 319
ML Y L + +A ++ G T D+ G +T E+T +I ++
Sbjct: 303 MLRYSFGLEKEAAAIEKAVDDVLQDGYCTGDLQVANGKVVSTIELTDRLIEKL 355
>gb|AAA22554.1| (M33099) 3-isopropylmalate dehydrogenase [Bacillus coagulans]
Length = 366
Score = 192 bits (482), Expect = 7e-48
Identities = 135/353 (38%), Positives = 194/353 (54%), Gaps = 37/353 (10%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYE-FEG---GFEVFKRIGSPISEDDLKEI 58
++AV+ GDGIGPEV+D+AIRV+ +V D FE G G+P+ E+ L
Sbjct: 4 KLAVLPGDGIGPEVMDAAIRVLKTVLDNDGHEAVFENALIGGAAIDEAGTPLPEETLDIC 63
Query: 59 RKMDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLRIIPDLSN---------- 103
R+ DAIL GA P SL ++ LRKE+ L+ANLR + +
Sbjct: 64 RRSDAILLGAVGGPKWDHNPASLRPEKGLLGLRKEMGLFANLRPVKAYATLLNASPLKRE 123
Query: 104 ---GKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN 155
++VIVRE T GLY R G G + +D T E RI + A LA+ R
Sbjct: 124 RVENVDLVIVRELTGGLYFGRPSERRGPG-ENEVVDTLAYTREEIERIIEKAFQLAQIRR 182
Query: 156 SFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTEN 214
+ V KANVL+ R +REI E A++ VE+ ++DS ++L+ NP VI+TEN
Sbjct: 183 KKLASVDKANVLESSRMWREIAEETAKKYPDVELSHMLVDSTSMQLIANPGQFDVIVTEN 242
Query: 215 MFGDIISDLATIHAGSIGIVPSGNY-GDEIALFEPIHGSAPDIAGKGIANPIGAILSAAM 273
MFGDI+SD A++ GS+G++PS + D ++EP+HGSAPDIAG+G ANP+G +LSAA+
Sbjct: 243 MFGDILSDEASVITGSLGMLPSASLRSDRFGMYEPVHGSAPDIAGQGKANPLGTVLSAAL 302
Query: 274 MLDY---LGLNGKVVWEATRRYVRYGNLTPDM----GGTATTSEVTKGIISEI 319
ML Y L + +A ++ G T D+ G +T E+T +I ++
Sbjct: 303 MLRYSFGLEKEAAAIEKAVDDVLQDGYCTGDLQVANGKVVSTIELTDRLIEKL 355
>sp|P43860|LEU3_HAEIN 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|1073789|pir||F64106 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Haemophilus influenzae
(strain Rd KW20) >gi|1574016|gb|AAC22648.1| (U32779)
3-isopropylmalate dehydrogenase (beta-IPM dehydrogenase)
(leuB) [Haemophilus influenzae Rd]
Length = 358
Score = 192 bits (482), Expect = 7e-48
Identities = 129/340 (37%), Positives = 185/340 (53%), Gaps = 34/340 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
Y +AV+ GDGIGPEV+ AI+V+N V ++ + F EF G + G P+ + LK
Sbjct: 4 YNIAVLAGDGIGPEVMAEAIKVLNRVQEKFGFKLNFNEFFVGGAAIEHCGYPLPAETLKG 63
Query: 58 IRKMDAILFGA------TTTPFNVPGYRSLIVTLRKELDLYANLR-------------II 98
+ DAILFG+ T P + R ++ LRK L+ NLR +
Sbjct: 64 CDQADAILFGSVGGPKWTNLPPDQQPERGALLPLRKHFKLFCNLRPATLYKGLEKFCPLR 123
Query: 99 PDLS-NGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAK 152
D++ G ++V+VRE T G+Y R+G G +A D + RIA+ A A
Sbjct: 124 ADIAAKGFDMVVVRELTGGIYFGQPKGREGDGVQTKAFDTEVYYKYEIERIARAAFEAAM 183
Query: 153 ERNSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVIL 211
+RN +T V KANVL+ +RE V E+A + V + ID+ ++L+K+P V+L
Sbjct: 184 KRNKKVTSVDKANVLQSSILWRETVTEMAKDYPEVTLEHIYIDNATMQLIKSPESFDVLL 243
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILS 270
N+FGDIISD A + GS+G++PS + +E L+EP GSAPDIAGKGIANPI ILS
Sbjct: 244 CSNIFGDIISDEAAMITGSMGMLPSASLNEEGFGLYEPAGGSAPDIAGKGIANPIAQILS 303
Query: 271 AAMMLDY---LGLNGKVVWEATRRYVRYGNLTPDMGGTAT 307
AAMML Y L + A ++ + G+ T D+ +T
Sbjct: 304 AAMMLRYSFNLNEAADAIESAVQKVLASGHRTADLADDST 343
>sp|P24098|LEU3_THEAQ 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|77580|pir||JX0173 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Thermus aquaticus
>gi|217178|dbj|BAA01542.1| (D10700)
threo-D-3-isopropylmalate dehydrogenase, alpha-2 subunit
[Thermus aquaticus]
Length = 344
Score = 191 bits (481), Expect = 9e-48
Identities = 128/344 (37%), Positives = 194/344 (56%), Gaps = 28/344 (8%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKEI 58
RVAV+ GDGIGPEV ++A+RV+ ++ +R + + F G G P E K +
Sbjct: 2 RVAVLPGDGIGPEVTEAALRVLKALDEREGLGLTYETFPFGGAAIDGYGEPFPEVTRKGV 61
Query: 59 RKMDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLR---IIP----------D 100
+A+L G+ P R + ++ LRK DL+ANLR + P +
Sbjct: 62 EAAEAVLLGSVGGPKWDALPRKIRPESGLLALRKSQDLFANLRPAKVFPGLERLSPLKEE 121
Query: 101 LSNGKEIVIVRENTEGLYARDGIGFSD-RAIDFRIITLEGARRIAKFAINLAKERNSFIT 159
++ G +++IVRE T G+Y + G S+ A + + R+AK A A++R +T
Sbjct: 122 IARGVDVLIVRELTGGIYFGEPRGMSEAEAWNTERYSKPEVERVAKVAFEAARKRRRHLT 181
Query: 160 FVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGD 218
V KANVL+ F+R+ V E+ + V + +D+ + LVKNP V++T N+FGD
Sbjct: 182 SVDKANVLEVGEFWRKTVEEVHKGYPDVALDHQYVDAMAMHLVKNPARFDVVVTGNIFGD 241
Query: 219 IISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDY- 277
I+SDLA++ GS+G++PS + G +FEP+HGSAPDIAGKGIANP AILSAAMML++
Sbjct: 242 ILSDLASVLPGSLGLLPSASLGRGTPVFEPVHGSAPDIAGKGIANPTAAILSAAMMLEHA 301
Query: 278 LGL--NGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
GL + V A + +R PD+GG+A T T+ ++ +
Sbjct: 302 FGLVELARRVEAAVAKALR-ETPPPDLGGSAGTQAFTEEVLRHL 344
>dbj|BAA88179.1| (AP000836) ESTs C19133(E10008),AU058199(E50136), AU081337(E50136)
correspond to a region of the predicted gene.~Similar to
isocitrate dehydrogenase (X96727) [Oryza sativa]
Length = 362
Score = 188 bits (473), Expect = 7e-47
Identities = 128/335 (38%), Positives = 184/335 (54%), Gaps = 23/335 (6%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKMD 62
R + GDGIGPE+ +S +V N I + E G EV R S ++ + L+ +R+
Sbjct: 33 RATLFPGDGIGPEIAESVKQVFNVAGVPIEWEEHYVGTEVDPRTESFLTWESLESVRRNK 92
Query: 63 AILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRENTEG 116
L G TP G+RSL +TLRKEL LYAN+R L K +V +RENTEG
Sbjct: 93 VGLKGPMATPIG-KGHRSLNLTLRKELGLYANVRPCNSLPGYKTRYDDVNLVTIRENTEG 151
Query: 117 LYARDGIGFSDRAI-----DFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVL-KG 169
Y+ G + + +IIT + + R+A++A + AK ++ +HKAN++ K
Sbjct: 152 EYS----GLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTNGRERVSAIHKANIMRKT 207
Query: 170 DRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHA 228
D F + E+AE+ + E IID+ + LVKNP V++ N++GDIISDL
Sbjct: 208 DGLFLKCCREVAEKYPEIVYEEVIIDNCCMTLVKNPGLFDVLVMPNLYGDIISDLCAGLI 267
Query: 229 GSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLNGKV--V 285
G +G+ PS N G+ I L E +HGSAPDIAGK +ANP +LSA MML +L N + +
Sbjct: 268 GGLGLTPSCNIGEGGICLAEAVHGSAPDIAGKNLANPTALMLSAVMMLRHLQFNNQADRI 327
Query: 286 WEATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
A + + G T D+GG A+TS+ TK + I
Sbjct: 328 HNAILQTISEGKFRTADLGGKASTSDFTKAVCDHI 362
>sp|P94631|LEU3_CORGL 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|1694761|emb|CAA70767.1| (Y09578)
3-isopropylmalate dehydrogenase [Corynebacterium
glutamicum]
Length = 340
Score = 187 bits (470), Expect = 2e-46
Identities = 124/299 (41%), Positives = 177/299 (58%), Gaps = 22/299 (7%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKMD 62
++AVI GDGIGPEV A++V+N+V D I +++ G + + G ++++DL +R+ D
Sbjct: 2 KLAVIGGDGIGPEVTAEALKVLNAVRDDIETTDYDLGARRYLKNGELLTDEDLASLREHD 61
Query: 63 AILFGATTTPFNVPG---YRSLIVTLRKELDLYANLR-------IIPDLSN-GK-EIVIV 110
AIL GA P +VP R L++ +R LD + NLR + L N GK + V+V
Sbjct: 62 AILLGAIGAPGSVPPGILERGLLLKMRFALDHHVNLRPSKLYDGVESPLRNPGKIDFVVV 121
Query: 111 RENTEGLYARDGIGF-----SDRAIDFRIITLEGARRIAKFAINLAKERNSFITFVHKAN 165
RE TEG Y +G + A + + T GA R+ ++A LA+ R +T VHK N
Sbjct: 122 REGTEGAYTGNGGAIRVGTPHEIANETSVNTRYGAERVIRYAFELAQSRRKKLTLVHKTN 181
Query: 166 VL-KGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDL 223
VL G ++ V E+A E V V ID+ I LV +P VI+T+N+FGDI++D
Sbjct: 182 VLVHGGGLWQRTVDEVAKEYPEVAVDYNHIDAATIYLVTDPSRFDVIVTDNLFGDILTDE 241
Query: 224 ATIHAGSIGIVPSGNY---GDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLG 279
A +G IG+ SGN G ++FEP+HGSAPDIAG+GIA+P AILSAAM+L +LG
Sbjct: 242 AGAVSGGIGLAASGNIDATGTNPSMFEPVHGSAPDIAGQGIADPTAAILSAAMLLRHLG 300
>sp|P05644|LEU3_BACCA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|98095|pir||A26447 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Bacillus caldotenax
>gi|39406|emb|CAA28455.1| (X04762) 3-isopropylmalate
dehydrogenase (AA 1-366) [Bacillus caldotenax]
Length = 366
Score = 187 bits (470), Expect = 2e-46
Identities = 131/352 (37%), Positives = 185/352 (52%), Gaps = 39/352 (11%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRF---YEFEGGF---EVFKRIGSPISEDDL 55
YR+AV+ GDGIG EV A+ V+ +V IRF + FE G G+P+ E+ +
Sbjct: 4 YRIAVLPGDGIGKEVTSGAVEVLKAVG--IRFGHEFTFEYGLIGGAAIDEAGTPLPEETV 61
Query: 56 KEIRKMDAILFGATTTPF---NVPGYRSL--IVTLRKELDLYANLRII------------ 98
+ R+ DA+L GA P N P R ++ +RK+LDLYANLR +
Sbjct: 62 RLCRESDAVLLGAVGGPKWDDNPPHLRPEKGLLAIRKQLDLYANLRPVVCYDSLVSRSPL 121
Query: 99 -PDLSNGKEIVIVRENTEGLYARDGIGF----SDRAIDFRIITLEGARRIAKFAINLAKE 153
PDL G + VIVRE T G+Y ++A+D + E RI + A LA+
Sbjct: 122 KPDLVQGVDFVIVRELTGGIYFGQPSAVVENGEEKAVDTLLYKKEEIERIVRMAFELARG 181
Query: 154 RNSFITFVHKANVLKGDRFFREIVLEIAEREGVEVREAIIDSFMIKLVKNPWDHGVILTE 213
R +T V KANVL R +RE+ E+A E ++ ++L++ P VI+TE
Sbjct: 182 RRKKVTSVDKANVLSSSRLWREVAEEVANEFPDVTLEHMLVDMRMQLIRAPKQFDVIVTE 241
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNY-GDEIALFEPIHGSAPDIAGKGIANPIGAILSAA 272
NMFGDI+SD A++ +GS+G++PS + +L+EP+HGSAPDIAG ANPI AILSAA
Sbjct: 242 NMFGDILSDEASMLSGSLGMLPSASLSASGPSLYEPVHGSAPDIAGMNKANPIAAILSAA 301
Query: 273 MMLDY-LGLNGKV-----VWEATR--RYVRYGNLTPDMGGTATTSEVTKGII 316
MML GL + VW+A R G P + E+ ++
Sbjct: 302 MMLRLSFGLTAEAGGRARVWQALALGSGSRLGQRRPHLSTNEMVEEIKAAVL 353
>pir||T03406 probable isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) precursor -
common tobacco >gi|3021506|emb|CAA65502.1| (X96727)
isocitrate dehydrogenase (NAD+) [Nicotiana tabacum]
Length = 364
Score = 185 bits (465), Expect = 6e-46
Identities = 122/337 (36%), Positives = 187/337 (55%), Gaps = 23/337 (6%)
Query: 1 MYRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRK 60
+ R + GDGIGPE+ DS ++ + I + E G E+ R S ++ + L+ +R+
Sbjct: 33 LIRATLFPGDGIGPEIADSVRQIFKTAEVPIEWEEHYVGKEIDPRTNSFLTWESLESVRR 92
Query: 61 MDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRENT 114
L G TP G+RSL +TLRKEL+LYAN+R L K ++ +RENT
Sbjct: 93 NKVGLKGPMATPIG-KGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENT 151
Query: 115 EGLYARDGIGFSDRAI-----DFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVL- 167
EG Y+ G + + +IIT + + R+A++A + AK ++ +HKAN++
Sbjct: 152 EGEYS----GVEHQVVRGVVESLKIITRQASLRVAEYAFHYAKAHGRERVSAIHKANIMQ 207
Query: 168 KGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATI 226
K D F + E+AE+ ++ E +ID+ + LVKNP +++ N++GDIISDL
Sbjct: 208 KTDGLFLKCCREVAEKYPEIKYEEVVIDNCCMMLVKNPALFDILVMPNLYGDIISDLCAG 267
Query: 227 HAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLNGKV- 284
G +G+ PS N G+ IAL E +HGSAPDIAGK +ANP +LSA ML +L L+ K
Sbjct: 268 VIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSAVTMLRHLELHDKAD 327
Query: 285 -VWEATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
+ A + G T D+GGT++T++ T I +
Sbjct: 328 RIQGAVLNTIAEGKYRTGDLGGTSSTTDFTNAICDHL 364
>pir||T48350 3-isopropylmalate dehydrogenase-like protein - Arabidopsis thaliana
>gi|7378609|emb|CAB83285.1| (AL162751) 3-isopropylmalate
dehydrogenase-like protein [Arabidopsis thaliana]
>gi|9757780|dbj|BAB08389.1| (AB005240) 3-isopropylmalate
dehydrogenase [Arabidopsis thaliana]
Length = 372
Score = 185 bits (464), Expect = 8e-46
Identities = 122/329 (37%), Positives = 184/329 (55%), Gaps = 23/329 (6%)
Query: 9 GDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKMDAILFGA 68
GDGIGPE+ +S +V + I + E G E+ R S ++ + L+ +R+ L G
Sbjct: 49 GDGIGPEIAESVKKVFTTAGVPIEWEEHYVGTEIDPRTQSFLTWESLESVRRNKVGLKGP 108
Query: 69 TTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRENTEGLYARDG 122
TP G+RSL +TLRKEL+LYAN+R L K +++ +RENTEG Y+
Sbjct: 109 MATPIG-KGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYS--- 164
Query: 123 IGFSDRAI-----DFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVL-KGDRFFRE 175
G + + +IIT + + R+A++A AK ++ +HKAN++ K D F +
Sbjct: 165 -GLEHQVVRGVVESLKIITRQASLRVAEYAFLYAKTHGRERVSAIHKANIMQKTDGLFLK 223
Query: 176 IVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGSIGIV 234
E+AE+ + E +ID+ + LVKNP V++ N++GDIISDL G +G+
Sbjct: 224 CCREVAEKYPEITYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLT 283
Query: 235 PSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLN--GKVVWEATRR 291
PS N G++ +AL E +HGSAPDIAGK +ANP +LS MML +L N + + A
Sbjct: 284 PSCNIGEDGVALAEAVHGSAPDIAGKNLANPTALLLSGVMMLRHLKFNEQAEQIHSAIIN 343
Query: 292 YVRYGNL-TPDMGGTATTSEVTKGIISEI 319
+ G T D+GG++TT+E TK I +
Sbjct: 344 TIAEGKYRTADLGGSSTTTEFTKAICDHL 372
>pdb|1XAC| Chimera Isopropylmalate Dehydrogenase Between Bacillus Subtilis
(M) And Thermus Thermophilus (T) From N-Terminal: 20% T
Middle 20% M Residual 60% T, Mutated At S82r. Low
Temperature (100k) Structure. >gi|1431784|pdb|1XAD|
Chimera Isopropylmalate Dehydrogenase Between Bacillus
Subtilis (M) And Thermus Thermophilus (T) From
N-Terminal: 20% T Middle 20% M Residual 60% T, Mutated
At S82r. Low Temperature (150k) Structure
Length = 345
Score = 185 bits (464), Expect = 8e-46
Identities = 122/344 (35%), Positives = 191/344 (55%), Gaps = 28/344 (8%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKEI 58
+VAV+ GDGIGPEV ++A++V+ ++ + + + F G G P E K +
Sbjct: 2 KVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKGV 61
Query: 59 RKMDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLR---IIPDLSNGK----- 105
+ +A+L G+ P R L ++++RK+LDL+ANLR + LS+
Sbjct: 62 EEAEAVLLGSVGGPKWDQNPRELRPEKGLLSIRKQLDLFANLRPVKVFESLSDASPLKKE 121
Query: 106 -----EIVIVRENTEGLYARDGIGFSD-RAIDFRIITLEGARRIAKFAINLAKERNSFIT 159
+ VIVRE T G+Y + G S+ A + + R+A+ A A++R +
Sbjct: 122 YIDNVDFVIVRELTGGIYFGEPRGMSEAEAWNTERYSKPEVERVARVAFEAARKRRKHVV 181
Query: 160 FVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGD 218
V KANVL+ F+R+ V E+ V + +D+ + LV++P V++T N+FGD
Sbjct: 182 SVDKANVLEVGEFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRSPARFDVVVTGNIFGD 241
Query: 219 IISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDY- 277
I+SDLA++ GS+G++PS + G +FEP+HGSAPDIAGKGIANP AILSAAMML++
Sbjct: 242 ILSDLASVLPGSLGLLPSASLGRGTPVFEPVHGSAPDIAGKGIANPTAAILSAAMMLEHA 301
Query: 278 LGL--NGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
GL + V +A + + PD+GG+A T T ++ +
Sbjct: 302 FGLVELARKVEDAVAKAL-LETPPPDLGGSAGTEAFTATVLRHL 344
>pdb|1OSJ|A Chain A, Structure Of 3-Isopropylmalate Dehydrogenase
>gi|1942482|pdb|1OSJ|B Chain B, Structure Of
3-Isopropylmalate Dehydrogenase
Length = 345
Score = 185 bits (464), Expect = 8e-46
Identities = 121/344 (35%), Positives = 193/344 (55%), Gaps = 28/344 (8%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKEI 58
+VAV+ GDGIGPEV ++A++V+ ++ + + + F G G P E K +
Sbjct: 2 KVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKGV 61
Query: 59 RKMDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLR---IIP----------D 100
+ +A+L G+ P R + +++LRK DL+ANLR + P +
Sbjct: 62 EEAEAVLLGSVGGPKWDGLPRKIRPETGLLSLRKSQDLFANLRPAKVFPGLERLSPLKEE 121
Query: 101 LSNGKEIVIVRENTEGLYARDGIGFSD-RAIDFRIITLEGARRIAKFAINLAKERNSFIT 159
++ G +++IVRE T G+Y + G S+ A + + R+A+ A LA++R +
Sbjct: 122 IARGVDVLIVRELTGGIYFGEPRGMSEAEAWNTERYSKPEVERVARVAFELARKRRKHVV 181
Query: 160 FVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGD 218
V KANVL+ F+R+ V E+ V + +D+ + LV++P V++T N+FGD
Sbjct: 182 SVDKANVLEVGEFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRSPARFDVVVTGNIFGD 241
Query: 219 IISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDY- 277
I+SDLA++ GS+G++PS + G +FEP+HGSAPDIAGKGIANP AILSAAMML++
Sbjct: 242 ILSDLASVLPGSLGLLPSASLGRGTPVFEPVHGSAPDIAGKGIANPTAAILSAAMMLEHA 301
Query: 278 LGL--NGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
GL + V +A + + PD+GG+A T T ++ +
Sbjct: 302 FGLVELARKVEDAVAKAL-LETPPPDLGGSAGTEAFTATVLRHL 344
>pdb|1DR0|A Chain A, Structure Of Modified 3-Isopropylmalate Dehydrogenase At
The C-Terminus, Hd708 >gi|6980527|pdb|1DR0|B Chain B,
Structure Of Modified 3-Isopropylmalate Dehydrogenase At
The C-Terminus, Hd708
Length = 346
Score = 184 bits (463), Expect = 1e-45
Identities = 121/344 (35%), Positives = 193/344 (55%), Gaps = 28/344 (8%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKEI 58
+VAV+ GDGIGPEV ++A++V+ ++ + + + F G G P E K +
Sbjct: 2 KVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKGV 61
Query: 59 RKMDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLR---IIP----------D 100
+ +A+L G+ P R + +++LRK DL+ANLR + P +
Sbjct: 62 EEAEAVLLGSVGGPKWDGLPRKIRPETGLLSLRKSQDLFANLRPAKVFPGLERLSPLKEE 121
Query: 101 LSNGKEIVIVRENTEGLYARDGIGFSD-RAIDFRIITLEGARRIAKFAINLAKERNSFIT 159
++ G +++IVRE T G+Y + G S+ A + + R+A+ A LA++R +
Sbjct: 122 IARGVDVLIVRELTGGIYFGEPRGMSEAEAWNTERYSKPEVERVARVAFELARKRRKHVV 181
Query: 160 FVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGD 218
V KANVL+ F+R+ V E+ V + +D+ + LV++P V++T N+FGD
Sbjct: 182 SVDKANVLEVGEFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRSPARFDVVVTGNIFGD 241
Query: 219 IISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDY- 277
I+SDLA++ GS+G++PS + G +FEP+HGSAPDIAGKGIANP AILSAAMML++
Sbjct: 242 ILSDLASVLPGSLGLLPSASLGRGTPVFEPVHGSAPDIAGKGIANPTAAILSAAMMLEHA 301
Query: 278 LGL--NGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
GL + V +A + + PD+GG+A T T + + +
Sbjct: 302 FGLVELARKVEDAVAKAL-LETPPPDLGGSAGTEAFTATVTATV 344
>pdb|1IDM| 3-Isopropylmalate Dehydrogenase, Loop-Deleted Chimera
Length = 343
Score = 184 bits (462), Expect = 1e-45
Identities = 120/342 (35%), Positives = 191/342 (55%), Gaps = 26/342 (7%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKEI 58
+VAV+ GDGIGPEV ++A++V+ ++ + + + F G G P E K +
Sbjct: 2 KVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKGV 61
Query: 59 RKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR---IIP----------DLS 102
+ +A+L G+ P G +++LRK DL+ANLR + P +++
Sbjct: 62 EEAEAVLLGSVGGPKWGTGSVRPEQGLLSLRKSQDLFANLRPAKVFPGLERLSPLKEEIA 121
Query: 103 NGKEIVIVRENTEGLYARDGIGFSD-RAIDFRIITLEGARRIAKFAINLAKERNSFITFV 161
G +++IVRE T G+Y + G S+ A + + R+A+ A A++R + V
Sbjct: 122 RGVDVLIVRELTGGIYFGEPRGMSEAEAWNTERYSKPEVERVARVAFEAARKRRKHVVSV 181
Query: 162 HKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDII 220
KANVL+ F+R+ V E+ V + +D+ + LV++P V++T N+FGDI+
Sbjct: 182 DKANVLEVGEFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRSPARFDVVVTGNIFGDIL 241
Query: 221 SDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDY-LG 279
SDLA++ GS+G++PS + G +FEP+HGSAPDIAGKGIANP AILSAAMML++ G
Sbjct: 242 SDLASVLPGSLGLLPSASLGRGTPVFEPVHGSAPDIAGKGIANPTAAILSAAMMLEHAFG 301
Query: 280 L--NGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
L + V +A + + PD+GG+A T T ++ +
Sbjct: 302 LVELARKVEDAVAKAL-LETPPPDLGGSAGTEAFTATVLRHL 342
>sp|P18869|LEU3_SCHPO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|7489952|pir||T39850 3-isopropylmalate
dehydrogenase - fission yeast (Schizosaccharomyces
pombe) >gi|11374698|pir||T43407 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) [validated] - fission yeast
(Schizosaccharomyces pombe) >gi|173412|gb|AAA35316.1|
(M36910) beta-isopropylmalate dehydrogenase (leu1)
(put.); putative [Schizosaccharomyces pombe]
>gi|3393014|emb|CAA20106.1| (AL031174) 3-isopropylmalate
dehydrogenase [Schizosaccharomyces pombe]
Length = 371
Score = 183 bits (461), Expect = 2e-45
Identities = 131/364 (35%), Positives = 199/364 (53%), Gaps = 48/364 (13%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDR-----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
++ V+ GD IGPE+V SA+ V+ V + + F E + G G+P++++ +K
Sbjct: 5 KIVVLPGDHIGPEIVASALEVLKVVEKKRPELKLEFEEHKIGGASIDAYGTPLTDETVKA 64
Query: 58 IRKMDAILFGATTTP-FNVPGYRSL--IVTLRKELDLYANLR--------------IIPD 100
+ D +L GA P + P R ++ LRK + ++ANLR + P+
Sbjct: 65 CLEADGVLLGAVGGPEWTNPNCRPEQGLLKLRKSMGVWANLRPCNFASKSLVKYSPLKPE 124
Query: 101 LSNGKEIVIVRENTEGLY--ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN--S 156
+ G + +VRE T G Y R S A+D +LE RIA+ A LA+ N +
Sbjct: 125 IVEGVDFCVVRELTGGCYFGERTEDNGSGYAMDTWPYSLEEVSRIARLAAWLAETSNPPA 184
Query: 157 FITFVHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGVILTE 213
+T + KANVL R +R+ V +I + E + ++ +IDS + LVK+P +GV+LT+
Sbjct: 185 PVTLLDKANVLATSRLWRKTVAKIFKEEYPHLTLKNQLIDSAAMLLVKSPRTLNGVVLTD 244
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNYGDEIA--------LFEPIHGSAPDIAGKGIANPI 265
N+FGDIISD A++ GS+G++PS + + L EPIHGSAPDIAGKGI NP+
Sbjct: 245 NLFGDIISDEASVIPGSLGLLPSASLSGVVGKSEEKVHCLVEPIHGSAPDIAGKGIVNPV 304
Query: 266 GAILSAAMMLDYLGLNGKVVWEATRRYVR----------YGNLTPDMGGTATTSEVTKGI 315
G ILSA+++L Y GLN EA VR G T D+GG A+T+++TK +
Sbjct: 305 GTILSASLLLRY-GLNAPKEAEAIEAAVRKVLDDTSIGGRGLYTRDLGGEASTADITKAV 363
Query: 316 ISEI 319
+ E+
Sbjct: 364 VEEL 367
>pdb|1DPZ|A Chain A, Stucture Of Modified 3-Isopropylmalate Dehydrogenase At
The C-Terminus, Hd711 >gi|6980659|pdb|1DPZ|B Chain B,
Stucture Of Modified 3-Isopropylmalate Dehydrogenase At
The C-Terminus, Hd711
Length = 349
Score = 183 bits (461), Expect = 2e-45
Identities = 121/337 (35%), Positives = 190/337 (55%), Gaps = 28/337 (8%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKEI 58
+VAV+ GDGIGPEV ++A++V+ ++ + + + F G G P E K +
Sbjct: 2 KVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKGV 61
Query: 59 RKMDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLR---IIP----------D 100
+ +A+L G+ P R + +++LRK DL+ANLR + P +
Sbjct: 62 EEAEAVLLGSVGGPKWDGLPRKIRPETGLLSLRKSQDLFANLRPAKVFPGLERLSPLKEE 121
Query: 101 LSNGKEIVIVRENTEGLYARDGIGFSD-RAIDFRIITLEGARRIAKFAINLAKERNSFIT 159
++ G +++IVRE T G+Y + G S+ A + + R+A+ A LA++R +
Sbjct: 122 IARGVDVLIVRELTGGIYFGEPRGMSEAEAWNTERYSKPEVERVARVAFELARKRRKHVV 181
Query: 160 FVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGD 218
V KANVL+ F+R+ V E+ V + +D+ + LV++P V++T N+FGD
Sbjct: 182 SVDKANVLEVGEFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRSPARFDVVVTGNIFGD 241
Query: 219 IISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDY- 277
I+SDLA++ GS+G++PS + G +FEP+HGSAPDIAGKGIANP AILSAAMML++
Sbjct: 242 ILSDLASVLPGSLGLLPSASLGRGTPVFEPVHGSAPDIAGKGIANPTAAILSAAMMLEHA 301
Query: 278 LGL--NGKVVWEATRRYVRYGNLTPDMGGTATTSEVT 312
GL + V +A + + PD+GG+A T T
Sbjct: 302 FGLVELARKVEDAVAKAL-LETPPPDLGGSAGTEAFT 337
>pdb|1DR8|A Chain A, Structure Of Modified 3-Isopropylmalate Dehydrogenase At
The C-Terminus, Hd177 >gi|6980529|pdb|1DR8|B Chain B,
Structure Of Modified 3-Isopropylmalate Dehydrogenase At
The C-Terminus, Hd177
Length = 344
Score = 183 bits (461), Expect = 2e-45
Identities = 121/337 (35%), Positives = 190/337 (55%), Gaps = 28/337 (8%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKEI 58
+VAV+ GDGIGPEV ++A++V+ ++ + + + F G G P E K +
Sbjct: 2 KVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKGV 61
Query: 59 RKMDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLR---IIP----------D 100
+ +A+L G+ P R + +++LRK DL+ANLR + P +
Sbjct: 62 EEAEAVLLGSVGGPKWDGLPRKIRPETGLLSLRKSQDLFANLRPAKVFPGLERLSPLKEE 121
Query: 101 LSNGKEIVIVRENTEGLYARDGIGFSD-RAIDFRIITLEGARRIAKFAINLAKERNSFIT 159
++ G +++IVRE T G+Y + G S+ A + + R+A+ A LA++R +
Sbjct: 122 IARGVDVLIVRELTGGIYFGEPRGMSEAEAWNTERYSKPEVERVARVAFELARKRRKHVV 181
Query: 160 FVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGD 218
V KANVL+ F+R+ V E+ V + +D+ + LV++P V++T N+FGD
Sbjct: 182 SVDKANVLEVGEFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRSPARFDVVVTGNIFGD 241
Query: 219 IISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDY- 277
I+SDLA++ GS+G++PS + G +FEP+HGSAPDIAGKGIANP AILSAAMML++
Sbjct: 242 ILSDLASVLPGSLGLLPSASLGRGTPVFEPVHGSAPDIAGKGIANPTAAILSAAMMLEHA 301
Query: 278 LGL--NGKVVWEATRRYVRYGNLTPDMGGTATTSEVT 312
GL + V +A + + PD+GG+A T T
Sbjct: 302 FGLVELARKVEDAVAKAL-LETPPPDLGGSAGTEAFT 337
>pdb|1G2U|A Chain A, The Structure Of The Mutant, A172v, Of 3-Isopropylmalate
Dehydrogenase From Thermus Thermophilus Hb8 : Its
Thermostability And Structure
Length = 345
Score = 183 bits (461), Expect = 2e-45
Identities = 120/344 (34%), Positives = 193/344 (55%), Gaps = 28/344 (8%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKEI 58
+VAV+ GDGIGPEV ++A++V+ ++ + + + F G G P E K +
Sbjct: 2 KVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKGV 61
Query: 59 RKMDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLR---IIP----------D 100
+ +A+L G+ P R + +++LRK DL+ANLR + P +
Sbjct: 62 EEAEAVLLGSVGGPKWDGLPRKIRPETGLLSLRKSQDLFANLRPAKVFPGLERLSPLKEE 121
Query: 101 LSNGKEIVIVRENTEGLYARDGIGFSD-RAIDFRIITLEGARRIAKFAINLAKERNSFIT 159
++ G +++IVRE T G+Y + G S+ A + + R+A+ A +A++R +
Sbjct: 122 IARGVDVLIVRELTGGIYFGEPRGMSEAEAWNTERYSKPEVERVARVAFEVARKRRKHVV 181
Query: 160 FVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGD 218
V KANVL+ F+R+ V E+ V + +D+ + LV++P V++T N+FGD
Sbjct: 182 SVDKANVLEVGEFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRSPARFDVVVTGNIFGD 241
Query: 219 IISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDY- 277
I+SDLA++ GS+G++PS + G +FEP+HGSAPDIAGKGIANP AILSAAMML++
Sbjct: 242 ILSDLASVLPGSLGLLPSASLGRGTPVFEPVHGSAPDIAGKGIANPTAAILSAAMMLEHA 301
Query: 278 LGL--NGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
GL + V +A + + PD+GG+A T T ++ +
Sbjct: 302 FGLVELARKVEDAVAKAL-LETPPPDLGGSAGTEAFTATVLRHL 344
>pdb|1GC8|A Chain A, The Crystal Structure Of Thermus Thermophilus 3-
Isopropylmalate Dehydrogenase Mutated At 172th From Ala
To Phe >gi|10835549|pdb|1GC8|B Chain B, The Crystal
Structure Of Thermus Thermophilus 3- Isopropylmalate
Dehydrogenase Mutated At 172th From Ala To Phe
Length = 345
Score = 183 bits (460), Expect = 2e-45
Identities = 120/344 (34%), Positives = 192/344 (54%), Gaps = 28/344 (8%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKEI 58
+VAV+ GDGIGPEV ++A++V+ ++ + + + F G G P E K +
Sbjct: 2 KVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKGV 61
Query: 59 RKMDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLR---IIP----------D 100
+ +A+L G+ P R + +++LRK DL+ANLR + P +
Sbjct: 62 EEAEAVLLGSVGGPKWDGLPRKIRPETGLLSLRKSQDLFANLRPAKVFPGLERLSPLKEE 121
Query: 101 LSNGKEIVIVRENTEGLYARDGIGFSD-RAIDFRIITLEGARRIAKFAINLAKERNSFIT 159
++ G +++IVRE T G+Y + G S+ A + + R+A+ A A++R +
Sbjct: 122 IARGVDVLIVRELTGGIYFGEPRGMSEAEAWNTERYSKPEVERVARVAFEFARKRRKHVV 181
Query: 160 FVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGD 218
V KANVL+ F+R+ V E+ V + +D+ + LV++P V++T N+FGD
Sbjct: 182 SVDKANVLEVGEFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRSPARFDVVVTGNIFGD 241
Query: 219 IISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDY- 277
I+SDLA++ GS+G++PS + G +FEP+HGSAPDIAGKGIANP AILSAAMML++
Sbjct: 242 ILSDLASVLPGSLGLLPSASLGRGTPVFEPVHGSAPDIAGKGIANPTAAILSAAMMLEHA 301
Query: 278 LGL--NGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
GL + V +A + + PD+GG+A T T ++ +
Sbjct: 302 FGLVELARKVEDAVAKAL-LETPPPDLGGSAGTEAFTATVLRHL 344
>sp|P00351|LEU3_THETH 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|443049|pdb|1IPD| 3-Isopropylmalate
Dehydrogenase (E.C.1.1.1.85) >gi|460072|gb|AAA16706.1|
(K01444) 3-isopropylmalate dehydrogenase [Thermus
thermophilus]
Length = 345
Score = 183 bits (459), Expect = 3e-45
Identities = 120/344 (34%), Positives = 192/344 (54%), Gaps = 28/344 (8%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKEI 58
+VAV+ GDGIGPEV ++A++V+ ++ + + + F G G P E K +
Sbjct: 2 KVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKGV 61
Query: 59 RKMDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLR---IIP----------D 100
+ +A+L G+ P R + +++LRK DL+ANLR + P +
Sbjct: 62 EEAEAVLLGSVGGPKWDGLPRKISPETGLLSLRKSQDLFANLRPAKVFPGLERLSPLKEE 121
Query: 101 LSNGKEIVIVRENTEGLYARDGIGFSD-RAIDFRIITLEGARRIAKFAINLAKERNSFIT 159
++ G +++IVRE T G+Y + G S+ A + + R+A+ A A++R +
Sbjct: 122 IARGVDVLIVRELTGGIYFGEPRGMSEAEAWNTERYSKPEVERVARVAFEAARKRRKHVV 181
Query: 160 FVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGD 218
V KANVL+ F+R+ V E+ V + +D+ + LV++P V++T N+FGD
Sbjct: 182 SVDKANVLEVGEFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRSPARFDVVVTGNIFGD 241
Query: 219 IISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDY- 277
I+SDLA++ GS+G++PS + G +FEP+HGSAPDIAGKGIANP AILSAAMML++
Sbjct: 242 ILSDLASVLPGSLGLLPSASLGRGTPVFEPVHGSAPDIAGKGIANPTAAILSAAMMLEHA 301
Query: 278 LGL--NGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
GL + V +A + + PD+GG+A T T ++ +
Sbjct: 302 FGLVELARKVEDAVAKAL-LETPPPDLGGSAGTEAFTATVLRHL 344
>pir||S41223 3-isopropylmalate dehydrogenase - Thermus aquaticus
>gi|7427675|pir||DETWIT 3-isopropylmalate dehydrogenase
(EC 1.1.1.85) - Thermus aquaticus >gi|1431780|pdb|1XAA|
3-Isopropylmalate Dehydrogenase, Low Temperature (100k)
Structure >gi|1431782|pdb|1XAB| 3-Isopropylmalate
Dehydrogenase, Low Temperature (150k) Structure
>gi|809121|pdb|1HEX| 3-Isopropylmalate Dehydrogenase
(E.C.1.1.1.85) Complexed With Beta-Nicotinamide Adenine
Dinucleotide, Oxidized (Nad+) >gi|1942477|pdb|1OSI|A
Chain A, Structure Of 3-Isopropylmalate Dehydrogenase
>gi|1942478|pdb|1OSI|B Chain B, Structure Of
3-Isopropylmalate Dehydrogenase >gi|1942479|pdb|1OSI|C
Chain C, Structure Of 3-Isopropylmalate Dehydrogenase
>gi|1942480|pdb|1OSI|D Chain D, Structure Of
3-Isopropylmalate Dehydrogenase
Length = 345
Score = 183 bits (459), Expect = 3e-45
Identities = 120/344 (34%), Positives = 192/344 (54%), Gaps = 28/344 (8%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKEI 58
+VAV+ GDGIGPEV ++A++V+ ++ + + + F G G P E K +
Sbjct: 2 KVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKGV 61
Query: 59 RKMDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLR---IIP----------D 100
+ +A+L G+ P R + +++LRK DL+ANLR + P +
Sbjct: 62 EEAEAVLLGSVGGPKWDGLPRKIRPETGLLSLRKSQDLFANLRPAKVFPGLERLSPLKEE 121
Query: 101 LSNGKEIVIVRENTEGLYARDGIGFSD-RAIDFRIITLEGARRIAKFAINLAKERNSFIT 159
++ G +++IVRE T G+Y + G S+ A + + R+A+ A A++R +
Sbjct: 122 IARGVDVLIVRELTGGIYFGEPRGMSEAEAWNTERYSKPEVERVARVAFEAARKRRKHVV 181
Query: 160 FVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGD 218
V KANVL+ F+R+ V E+ V + +D+ + LV++P V++T N+FGD
Sbjct: 182 SVDKANVLEVGEFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRSPARFDVVVTGNIFGD 241
Query: 219 IISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDY- 277
I+SDLA++ GS+G++PS + G +FEP+HGSAPDIAGKGIANP AILSAAMML++
Sbjct: 242 ILSDLASVLPGSLGLLPSASLGRGTPVFEPVHGSAPDIAGKGIANPTAAILSAAMMLEHA 301
Query: 278 LGL--NGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
GL + V +A + + PD+GG+A T T ++ +
Sbjct: 302 FGLVELARKVEDAVAKAL-LETPPPDLGGSAGTEAFTATVLRHL 344
>pdb|1WAL|A Chain A, 3-Isopropylmalate Dehydrogenase (Ipmdh) Mutant (M219a)from
Thermus Thermophilus
Length = 345
Score = 182 bits (457), Expect = 6e-45
Identities = 120/344 (34%), Positives = 192/344 (54%), Gaps = 28/344 (8%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKEI 58
+VAV+ GDGIGPEV ++A++V+ ++ + + + F G G P E K +
Sbjct: 2 KVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKGV 61
Query: 59 RKMDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLR---IIP----------D 100
+ +A+L G+ P R + +++LRK DL+ANLR + P +
Sbjct: 62 EEAEAVLLGSVGGPKWDGLPRKISPETGLLSLRKSQDLFANLRPAKVFPGLERLSPLKEE 121
Query: 101 LSNGKEIVIVRENTEGLYARDGIGFSD-RAIDFRIITLEGARRIAKFAINLAKERNSFIT 159
++ G +++IVRE T G+Y + G S+ A + + R+A+ A A++R +
Sbjct: 122 IARGVDVLIVRELTGGIYFGEPRGMSEAEAWNTERYSKPEVERVARVAFEAARKRRKHVV 181
Query: 160 FVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGD 218
V KANVL+ F+R+ V E+ V + +D+ + LV++P V++T N+FGD
Sbjct: 182 SVDKANVLEVGEFWRKTVEEVGRGYPDVALEHQYVDAAAMHLVRSPARFDVVVTGNIFGD 241
Query: 219 IISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDY- 277
I+SDLA++ GS+G++PS + G +FEP+HGSAPDIAGKGIANP AILSAAMML++
Sbjct: 242 ILSDLASVLPGSLGLLPSASLGRGTPVFEPVHGSAPDIAGKGIANPTAAILSAAMMLEHA 301
Query: 278 LGL--NGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
GL + V +A + + PD+GG+A T T ++ +
Sbjct: 302 FGLVELARKVEDAVAKAL-LETPPPDLGGSAGTEAFTATVLRHL 344
>pdb|1GC9|A Chain A, The Crystal Structure Of Thermus Thermophilus 3-
Isopropylmalate Dehydrogenase Mutated At 172th From Ala
To Gly
Length = 345
Score = 182 bits (456), Expect = 7e-45
Identities = 120/344 (34%), Positives = 192/344 (54%), Gaps = 28/344 (8%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKEI 58
+VAV+ GDGIGPEV ++A++V+ ++ + + + F G G P E K +
Sbjct: 2 KVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKGV 61
Query: 59 RKMDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLR---IIP----------D 100
+ +A+L G+ P R + +++LRK DL+ANLR + P +
Sbjct: 62 EEAEAVLLGSVGGPKWDGLPRKIRPETGLLSLRKSQDLFANLRPAKVFPGLERLSPLKEE 121
Query: 101 LSNGKEIVIVRENTEGLYARDGIGFSD-RAIDFRIITLEGARRIAKFAINLAKERNSFIT 159
++ G +++IVRE T G+Y + G S+ A + + R+A+ A A++R +
Sbjct: 122 IARGVDVLIVRELTGGIYFGEPRGMSEAEAWNTERYSKPEVERVARVAFEGARKRRKHVV 181
Query: 160 FVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGD 218
V KANVL+ F+R+ V E+ V + +D+ + LV++P V++T N+FGD
Sbjct: 182 SVDKANVLEVGEFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRSPARFDVVVTGNIFGD 241
Query: 219 IISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDY- 277
I+SDLA++ GS+G++PS + G +FEP+HGSAPDIAGKGIANP AILSAAMML++
Sbjct: 242 ILSDLASVLPGSLGLLPSASLGRGTPVFEPVHGSAPDIAGKGIANPTAAILSAAMMLEHA 301
Query: 278 LGL--NGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
GL + V +A + + PD+GG+A T T ++ +
Sbjct: 302 FGLVELARKVEDAVAKAL-LETPPPDLGGSAGTEAFTATVLRHL 344
>gb|AAK41021.1| 3-isopropylmalate dehydrogenase (leuB) [Sulfolobus solfataricus]
Length = 336
Score = 181 bits (455), Expect = 1e-44
Identities = 127/344 (36%), Positives = 185/344 (52%), Gaps = 34/344 (9%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
+RVAVI GDGIGPE+ + + R++ + ++ I E E G + G + L
Sbjct: 3 FRVAVIPGDGIGPELYEGSKRIIAKLIEKYNLDIDLIEVEAGDVALNKYGEALPRHTLDV 62
Query: 58 IRKMDAILFGAT-TTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIV 110
I K D IL G T +V +V LR+ D+YAN+R L N + ++VIV
Sbjct: 63 IEKADMILKGPVGETAMDV------VVKLRQMYDMYANIRPAKSLPNVQSKYPNVDLVIV 116
Query: 111 RENTEGLYAR-DGIGFSDRAIDFRIITLEGARRIAKFAINLAKERNSFITFVHKANVLK- 168
RENTE LY + + + I ++ T ++RI A+N A R +T VHKANV++
Sbjct: 117 RENTEDLYKGFEFVTSTGVTIAIKVTTEFASKRIVNVALNYALMRRRKVTCVHKANVMRV 176
Query: 169 GDRFFREIVLEIAEREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHA 228
D F + EI + + V E +D+ LV++P VI+T N++GDI+SD A+ A
Sbjct: 177 TDGLFASVCREILKGK-VNFEEMYVDAAAANLVRDPTRFDVIVTSNVYGDILSDEASQIA 235
Query: 229 GSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLG--------- 279
GS+G+ PS N GD ++FEP+HG+A DIAGKGIANP +LS +MML +
Sbjct: 236 GSLGLAPSANIGDRKSMFEPVHGAAFDIAGKGIANPTAFLLSVSMMLSRMYELSKENKYI 295
Query: 280 LNGKVVWEATRRYVRYGN-LTPDMGGTATTSEVTKGIISEIYSF 322
L K + A + GN LT D+GG+A K ++ EIY +
Sbjct: 296 LASKSLENAIIETYKSGNKLTEDVGGSAK----LKDMVDEIYKY 335
>emb|CAB57580.1| (Y18930) 3-isopropylmalate dehydrogenase [Sulfolobus solfataricus]
Length = 336
Score = 181 bits (455), Expect = 1e-44
Identities = 127/344 (36%), Positives = 185/344 (52%), Gaps = 34/344 (9%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
+RVAVI GDGIGPE+ + + R++ + ++ I E E G + G + L
Sbjct: 3 FRVAVIPGDGIGPELYEGSKRIIAKLIEKYNLDIDLIEVEAGDVALNKYGEALPRHTLDV 62
Query: 58 IRKMDAILFGAT-TTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIV 110
I K D IL G T +V +V LR+ D+YAN+R L N + ++VIV
Sbjct: 63 IEKADMILKGPVGETAMDV------VVKLRQMYDMYANIRPAKSLPNVQSKYPNVDLVIV 116
Query: 111 RENTEGLYAR-DGIGFSDRAIDFRIITLEGARRIAKFAINLAKERNSFITFVHKANVLK- 168
RENTE LY + + + I ++ T ++RI A+N A R +T VHKANV++
Sbjct: 117 RENTEDLYKGFEFVTSTGVTIAIKVTTEFASKRIVNVALNYALMRRRKVTCVHKANVMRV 176
Query: 169 GDRFFREIVLEIAEREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHA 228
D F + EI + + V E +D+ LV++P VI+T N++GDI+SD A+ A
Sbjct: 177 TDGLFASVCREILKGK-VNFEEMYVDAAAANLVRDPTRFDVIVTSNVYGDILSDEASQIA 235
Query: 229 GSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLG--------- 279
GS+G+ PS N GD ++FEP+HG+A DIAGKGIANP +LS +MML +
Sbjct: 236 GSLGLAPSANIGDRKSMFEPVHGAAFDIAGKGIANPTAFLLSVSMMLSRMYELSKENKYI 295
Query: 280 LNGKVVWEATRRYVRYGN-LTPDMGGTATTSEVTKGIISEIYSF 322
L K + A + GN LT D+GG+A K ++ EIY +
Sbjct: 296 LASKSLENAIIETYKSGNKLTEDVGGSAK----LKDMVDEIYKY 335
>pir||A26522 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Bacillus subtilis
>gi|580891|emb|CAA68432.1| (Y00353) 3-isopropylmalate
dehydrogenase (AA 1 - 365) [Bacillus subtilis]
Length = 365
Score = 180 bits (453), Expect = 2e-44
Identities = 128/356 (35%), Positives = 188/356 (51%), Gaps = 40/356 (11%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRF-YEFEGGF---EVFKRIGSPISEDDLKEI 58
R+A++ GDGIGPEV++SA V+ SV +R +EFE G +P+ E+ +
Sbjct: 4 RIALLPGDGIGPEVLESATDVLKSVAERFNHEFEFEYGLIGGAAIDEHHNPLPEETVAAC 63
Query: 59 RKMDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLR---IIPDLSNGK----- 105
+ DAIL GA P L ++++RK+LDL+ANLR + LS+
Sbjct: 64 KNADAILLGAVGGPKWDQNLSELRPEKGLLSIRKQLDLFANLRPVKVFESLSDRSPLKKE 123
Query: 106 -----EIVIVRENTEGLYARD------GIGFSDRAIDFRIITLEGARRIAKFAINLAKER 154
+ VIVRE T GLY A+D R+ + +A R
Sbjct: 124 YIDNVDFVIVRELTGGLYFGQPSKRYVNTEGEQEAVDTLFYKRTEIERVIREGFKMAATR 183
Query: 155 NSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTE 213
+T V KANVL+ R +RE+ ++A+ V++ ++D+ ++L+ P V++TE
Sbjct: 184 KGKVTSVDKANVLESSRLWREVAEDVAQEFPDVKLEHMLVDNAAMQLIYAPNQFDVVVTE 243
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILSAA 272
NMFGDI+SD A++ GS+G++PS + + LFEP+HGSAPDIAGKG+ANP AILSAA
Sbjct: 244 NMFGDILSDEASMLTGSLGMLPSASLSSSGLHLFEPVHGSAPDIAGKGMANPFAAILSAA 303
Query: 273 MML-DYLGL--NGKVVWEATRRYVRYGNLTPDMGGT-------ATTSEVTKGIISE 318
M+L GL K V +A + + G T D+ + A T EV I+SE
Sbjct: 304 MLLRTSFGLEEEAKAVEDAVNKVLASGKRTRDLARSEEFSSTQAITEEVKAAIMSE 359
>sp|P05645|LEU3_BACSU 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|1770070|emb|CAA99532.1| (Z75208)
3-isopropylmalate dehydrogenase [Bacillus subtilis]
>gi|2635292|emb|CAB14787.1| (Z99118) 3-isopropylmalate
dehydrogenase [Bacillus subtilis]
Length = 365
Score = 180 bits (453), Expect = 2e-44
Identities = 128/356 (35%), Positives = 188/356 (51%), Gaps = 40/356 (11%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRF-YEFEGGF---EVFKRIGSPISEDDLKEI 58
R+A++ GDGIGPEV++SA V+ SV +R +EFE G +P+ E+ +
Sbjct: 4 RIALLPGDGIGPEVLESATDVLKSVAERFNHEFEFEYGLIGGAAIDEHHNPLPEETVAAC 63
Query: 59 RKMDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLR---IIPDLSNGK----- 105
+ DAIL GA P L ++++RK+LDL+ANLR + LS+
Sbjct: 64 KNADAILLGAVGGPKWDQNLSELRPEKGLLSIRKQLDLFANLRPVKVFESLSDRSPLKKE 123
Query: 106 -----EIVIVRENTEGLYARD------GIGFSDRAIDFRIITLEGARRIAKFAINLAKER 154
+ VIVRE T GLY A+D R+ + +A R
Sbjct: 124 YIDNVDFVIVRELTGGLYFGQPSKRYVNTEGEQEAVDTLFYKRTEIERVIREGFKMAAAR 183
Query: 155 NSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTE 213
+T V KANVL+ R +RE+ ++A+ V++ ++D+ ++L+ P V++TE
Sbjct: 184 KGKVTSVDKANVLESSRLWREVAEDVAQEFPDVKLEHMLVDNAAMQLIYAPNQFDVVVTE 243
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILSAA 272
NMFGDI+SD A++ GS+G++PS + + LFEP+HGSAPDIAGKG+ANP AILSAA
Sbjct: 244 NMFGDILSDEASMLTGSLGMLPSASLSSSGLHLFEPVHGSAPDIAGKGMANPFAAILSAA 303
Query: 273 MML-DYLGL--NGKVVWEATRRYVRYGNLTPDMGGT-------ATTSEVTKGIISE 318
M+L GL K V +A + + G T D+ + A T EV I+SE
Sbjct: 304 MLLRTSFGLEEEAKAVEDAVNKVLASGKRTRDLARSEEFSSTQAITEEVKAAIMSE 359
>pir||G75355 3-isopropylmalate dehydrogenase - Deinococcus radiodurans (strain
R1) >gi|6459553|gb|AAF11333.1|AE002019_6 (AE002019)
3-isopropylmalate dehydrogenase [Deinococcus
radiodurans]
Length = 352
Score = 180 bits (453), Expect = 2e-44
Identities = 123/348 (35%), Positives = 183/348 (52%), Gaps = 28/348 (8%)
Query: 1 MYRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRK 60
M +V + GDGIGPEV +A++V+ V + E G + G P +++
Sbjct: 1 MPKVITLPGDGIGPEVTAAAVQVLREVAPDVTVEEHAIGGGAYDAHGEPFPTSTRDALKE 60
Query: 61 MDAILFG----ATTTPFN-VPGY---RSLIVTLRKELDLYANLRII-------------P 99
DA+L G A + +N +P + S ++ LRK L YANLR +
Sbjct: 61 ADAVLLGTVGGAHDSAWNQLPRHLRPESGLLALRKALGCYANLRPVRVQPGLEHLSPLKA 120
Query: 100 DLSNGKEIVIVRENTEGLYA-RDGIGFSDRAIDFRIITLEGARRIAKFAINLAKERNSFI 158
+L+ G +I+IVRE G+Y +D D A + T R+AK A A++R +
Sbjct: 121 ELARGVDILIVRELLGGIYFDQDRKIEGDTAYNTMRYTTSEVERVAKVAFWAAEQRKGRV 180
Query: 159 TFVHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWDHGVILTENMF 216
T V KANVL+ +R V + +R+ + + +DS + +V +P + VILTEN+F
Sbjct: 181 TSVDKANVLEVSELWRRDVQALRDRDYRSIHLNHEYVDSVAMLIVADPSRYDVILTENLF 240
Query: 217 GDIISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLD 276
GDI+SDLA + GS+G++PS + GD LFEPIHGSAPDIAG+G+ANP AI+S M+L
Sbjct: 241 GDILSDLAAVIPGSLGLMPSASLGDGAGLFEPIHGSAPDIAGQGVANPAAAIMSVGMLLR 300
Query: 277 YLGLNGKVVWEATRRYVRYG---NLTPDMGGTATTSEVTKGIISEIYS 321
+ GL R V T D+GG+A T T+ ++ + S
Sbjct: 301 H-GLERSEAANQVDRAVALALREQPTRDLGGSADTQTFTRAVLKALGS 347
>sp|O34296|TTC3_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC' (TDH)
>gi|2305218|gb|AAB65748.1| (AF010263) tartrate
dehydrogenase [Agrobacterium vitis]
Length = 358
Score = 180 bits (451), Expect = 3e-44
Identities = 128/351 (36%), Positives = 190/351 (53%), Gaps = 35/351 (9%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR-----IRFYEFEGGFEVFKRIGSPISEDDLK 56
Y++A I DGIGPEV+ + ++V+ ++ R I F+ G + +K+ G + D L
Sbjct: 4 YKIAAIPADGIGPEVIAAGLQVLEALEQRSGDFKIHTETFDWGSDYYKKHGVMMPADGLD 63
Query: 57 EIRKMDAILFGATTTPFNVPGYRSLI---VTLRKELDLYANLR-------IIPDLSN--- 103
+++K DAI FGA P +VP + +L + + + D YAN+R I P L N
Sbjct: 64 KLKKFDAIFFGAVGAP-DVPDHITLWGLRLPICQGFDQYANVRPTKILPGITPPLRNCGP 122
Query: 104 -GKEIVIVRENTEGLYARDGIGFSDRAI------DFRIITLEGARRIAKFAINLAKER-N 155
+ VIVREN+EG Y+ G G + R + + I T G RI ++A LA+ R
Sbjct: 123 GDLDWVIVRENSEGEYSGHG-GRAHRGLPEEVGTEVAIFTRVGVTRIMRYAFKLAQARPR 181
Query: 156 SFITFVHKANVLK-GDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTE 213
+T V K+N + G + EI E+A E V + ++D+ +++ P I+
Sbjct: 182 KLLTVVTKSNAQRHGMVMWDEIAAEVATEFPDVTWDKMLVDAMTVRMTLKPETLDTIVAT 241
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNYGDEI---ALFEPIHGSAPDIAGKGIANPIGAILS 270
N+ DI+SDLA AGS+G+ P+ N E ++FEPIHGSA DI GKGIANPI +
Sbjct: 242 NLHADILSDLAGALAGSLGVAPTANIDPERRFPSMFEPIHGSAFDITGKGIANPIATFWT 301
Query: 271 AAMMLDYLGLNGKV--VWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
AA ML++LG + A R G LTPD+GGTA TS+VT+ + + I
Sbjct: 302 AAQMLEHLGERDAAARLMSAVERVTEAGILTPDVGGTANTSQVTEAVCNAI 352
>sp|O34295|TTC5_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC' (TDH)
>gi|2305214|gb|AAB65746.1| (AF010261) tartrate
dehydrogenase [Agrobacterium vitis]
Length = 358
Score = 179 bits (449), Expect = 5e-44
Identities = 128/351 (36%), Positives = 190/351 (53%), Gaps = 35/351 (9%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR-----IRFYEFEGGFEVFKRIGSPISEDDLK 56
Y++A I DGIGPEV+ + ++V+ ++ R I F+ G + +K+ G + D L
Sbjct: 4 YKIAAIPADGIGPEVIAAGLQVLEALEQRSGDFKIHTETFDWGSDYYKKHGVMMPADGLD 63
Query: 57 EIRKMDAILFGATTTPFNVPGYRSLI---VTLRKELDLYANLR-------IIPDLSN--- 103
+++K DAI FGA P +VP + +L + + + D YAN+R I P L N
Sbjct: 64 KLKKFDAIFFGAVGAP-DVPDHITLWGLRLPICQGFDQYANVRPTKILPGITPPLRNCGP 122
Query: 104 -GKEIVIVRENTEGLYARDGIGFSDRAI------DFRIITLEGARRIAKFAINLAKER-N 155
+ VIVREN+EG Y+ G G + R + + I T G RI ++A LA+ R
Sbjct: 123 GDLDWVIVRENSEGEYSGHG-GRAHRGLPEEVGTEVAIFTRVGVTRIMRYAFKLAQARPR 181
Query: 156 SFITFVHKANVLK-GDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTE 213
+T V K+N + G + EI E+A E V + ++D+ +++ P I+
Sbjct: 182 KLLTVVTKSNAQRHGMVMWDEIAAEVATEFPDVTWDKMLVDAMTVRMTLKPETLDTIVAT 241
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNYGDEI---ALFEPIHGSAPDIAGKGIANPIGAILS 270
N+ DI+SDLA AGS+G+ P+ N E ++FEPIHGSA DI GKGIANPI +
Sbjct: 242 NLHADILSDLAGALAGSLGVAPTANIDPERRFPSMFEPIHGSAFDITGKGIANPIATFWT 301
Query: 271 AAMMLDYLGLNGKV--VWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
AA ML++LG + A R G LTPD+GGTA TS+VT+ + + I
Sbjct: 302 AAQMLEHLGERDAAARLMGAVERVTEAGILTPDVGGTANTSQVTEAVCNAI 352
>gb|AAF23254.1|AC015985_12 (AC015985) putative isocitrate dehydrogenase (NAD+) [Arabidopsis
thaliana]
Length = 374
Score = 178 bits (447), Expect = 8e-44
Identities = 122/335 (36%), Positives = 185/335 (54%), Gaps = 23/335 (6%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKMD 62
+ + GDGIGPE+ +S +V + I + E G EV R S ++ D+L+ + K
Sbjct: 45 KATLFPGDGIGPEIAESVKQVFTAADVVIDWDEQFVGTEVDPRTNSFLTWDNLQSVLKNK 104
Query: 63 AILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRENTEG 116
L G TP G+RSL +TLRKEL+LYAN+R L K +++ +RENTEG
Sbjct: 105 VGLKGPMATPIG-KGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEG 163
Query: 117 LYARDGIGFSDRAI-----DFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVL-KG 169
Y+ G + + +IIT + + R+A++A AK ++ +HKAN++ K
Sbjct: 164 EYS----GLEHQVVKGVVESLKIITRKASMRVAEYAFLYAKTHGRKKVSAIHKANIMQKT 219
Query: 170 DRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHA 228
D F + E+A + + + +ID+ + LVKNP V++ N++GDIISDL
Sbjct: 220 DGLFLQCCDEVAAKYPEIYYEKVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLV 279
Query: 229 GSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLN--GKVV 285
G +G+ PS N G++ IAL E +HGSAPDIAG +ANP +LS MML +L LN + +
Sbjct: 280 GGLGLTPSMNIGEDGIALAEAVHGSAPDIAGMNLANPTALLLSGVMMLRHLKLNKQAEQI 339
Query: 286 WEATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
A + G T D+GG++TT++ TK I +
Sbjct: 340 HSAIINTIAEGKYRTADLGGSSTTTDFTKAICDHL 374
>sp|Q44471|TTC1_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC (TDH)
>gi|805292|gb|AAA68698.1| (U25634) putative tartrate
dehydrogenase; already transcribed in the absence of
tartrate and induced when trartrate is added; Method:
conceptual translation supplied by author [Agrobacterium
vitis] >gi|2305216|gb|AAB65747.1| (AF010262) tartrate
dehydrogenase [Agrobacterium vitis]
>gi|1585263|prf||2124372C ttuC gene [Agrobacterium
vitis]
Length = 364
Score = 178 bits (446), Expect = 1e-43
Identities = 127/351 (36%), Positives = 189/351 (53%), Gaps = 35/351 (9%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR-----IRFYEFEGGFEVFKRIGSPISEDDLK 56
Y++A I DGIGPEV+ + ++V+ ++ R I F+ G + +K+ G + D L
Sbjct: 4 YKIAAIPADGIGPEVIAAGLQVLEALEQRSGDFKIHTETFDWGSDYYKKHGVMMPADGLD 63
Query: 57 EIRKMDAILFGATTTPFNVPGYRSLI---VTLRKELDLYANLR-------IIPDLSN--- 103
+++K DAI FGA P +VP + +L + + + D YAN+R I P L N
Sbjct: 64 KLKKFDAIFFGAVGAP-DVPDHITLWGLRLPICQGFDQYANVRPTKILPGITPPLRNCGP 122
Query: 104 -GKEIVIVRENTEGLYARDGIGFSDRAI------DFRIITLEGARRIAKFAINLAKER-N 155
+ VIVREN+EG Y+ G G + R + + I T G RI ++A LA+ R
Sbjct: 123 GDLDWVIVRENSEGEYSGHG-GRAHRGLPEEVGTEVAIFTRVGVTRIMRYAFKLAQARPR 181
Query: 156 SFITFVHKANVLK-GDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTE 213
+T V K+N + G + EI E+A E V + ++D+ +++ P I+
Sbjct: 182 KLLTVVTKSNAQRHGMVMWDEIAAEVATEFPDVTWDKMLVDAMTVRMTLKPETLDTIVAT 241
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNYGDEI---ALFEPIHGSAPDIAGKGIANPIGAILS 270
N+ DI+SDLA AGS+G+ P+ N E ++FEPIHGSA DI GKGIANPI +
Sbjct: 242 NLHADILSDLAGALAGSLGVAPTANIDPERRFPSMFEPIHGSAFDITGKGIANPIATFWT 301
Query: 271 AAMMLDYLGLNGKV--VWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
AA ML++LG + A R G LTPD+GGTA T +VT+ + + I
Sbjct: 302 AAQMLEHLGERDAAARLMSAVERVTEAGILTPDVGGTANTRQVTEAVCNAI 352
>pir||G82070 3-isopropylmalate dehydrogenase VC2491 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9657071|gb|AAF95633.1|
(AE004318) 3-isopropylmalate dehydrogenase [Vibrio
cholerae]
Length = 363
Score = 178 bits (446), Expect = 1e-43
Identities = 124/339 (36%), Positives = 184/339 (53%), Gaps = 36/339 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR--IRFYEFE---GGFEVFKRIGSPISEDDLK 56
Y++AV+ GDGIGPEV+ A +V++++ + IRF E GG + G P+ E L+
Sbjct: 6 YKIAVLPGDGIGPEVMAQAHKVLDAIEQKHGIRFSREEHDVGGIAIDNH-GCPLPESTLR 64
Query: 57 EIRKMDAILFGATTTPF------NVPGYRSLIVTLRKELDLYANLR-------------I 97
+ DA+LFG+ P N R ++ LRK L+ NLR +
Sbjct: 65 ACEEADAVLFGSVGGPKWEHLPPNEQPERGALLPLRKHFQLFCNLRPAQIHQGLEAFSPL 124
Query: 98 IPDLS-NGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLA 151
D+S G +IV+VRE T G+Y R+G G ++A D + RIA+ A A
Sbjct: 125 RADISARGFDIVVVRELTGGIYFGQPKGREGEGAHEKAFDTEVYHRFEIERIARIAFESA 184
Query: 152 KERNSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVI 210
+ R + + KANVL+ +RE+V EIA E V + ID+ ++L+K+P V+
Sbjct: 185 RLRRKKVCSIDKANVLQSSILWREVVSEIAKEYPDVSLSHMYIDNATMQLIKDPAQFDVM 244
Query: 211 LTENMFGDIISDLATIHAGSIGIVPSGNYGD-EIALFEPIHGSAPDIAGKGIANPIGAIL 269
L N+FGDI+SD + GS+G++PS + + + L+EP GSAPDIAGK IANP+ IL
Sbjct: 245 LCSNIFGDILSDECAMITGSMGMLPSASMNESKFGLYEPAGGSAPDIAGKNIANPVAQIL 304
Query: 270 SAAMMLDY-LG--LNGKVVWEATRRYVRYGNLTPDMGGT 305
SAA+ML Y LG + + A + + G LT D+ G+
Sbjct: 305 SAALMLRYSLGEEAAARDIENAVSQALAAGELTADLAGS 343
>sp|P33197|IDH_THETH ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCCINATE DECARBOXYLASE)
(IDH) (NADP+-SPECIFIC ICDH) (IDP)
>gi|155102|gb|AAA27492.1| (M94317) isocitrate
dehydrogenase [Thermus thermophilus]
Length = 495
Score = 177 bits (445), Expect = 1e-43
Identities = 123/357 (34%), Positives = 191/357 (53%), Gaps = 34/357 (9%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKR-IGSPISEDDLKEIRKMD 62
+ VI GDGIGPE V++ ++V+ + + + E G VF+R I S + ++ ++ IRK
Sbjct: 22 ITVIPGDGIGPECVEATLKVLEAAKAPLAYEVREAGASVFRRGIASGVPQETIESIRKTR 81
Query: 63 AILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSN--------GKEIVIVRENT 114
+L G TP G +S VTLRK + YAN+R + + N G ++V+VREN
Sbjct: 82 VVLKGPLETPVGY-GEKSANVTLRKLFETYANVRPVREFPNVPTPYAGRGIDLVVVRENV 140
Query: 115 EGLYAR-DGIGFSDRAIDFRIITLEGARRIAKFAINLAK-ERNSFITFVHKANVLK---- 168
E LYA + + A ++I+ +G+ +I +FA LA+ E + K+N++K
Sbjct: 141 EDLYAGIEHMQTPSVAQTLKLISWKGSEKIVRFAFELARAEGRKKVHCATKSNIMKLAEG 200
Query: 169 GDRFFREIVLEIAEREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHA 228
R F ++ E + E V + I+D+ +LVK P VI+T NM GDI+SDL +
Sbjct: 201 PKRAFEQVAQEYPDIEAVHI---IVDNAAHQLVKRPEQFEVIVTTNMNGDILSDLTSGLI 257
Query: 229 GSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLG--LNGKVVW 286
G +G PS N G+E+A+FE +HGSAP AGK + NP +LSA MML YL ++
Sbjct: 258 GGLGFAPSANIGNEVAIFEAVHGSAPKYAGKNVINPTAVLLSAVMMLRYLEEFATADLIE 317
Query: 287 EATRRYVRYGN-LTPDMGG---TATTSEVTKGII---------SEIYSFDPFDIDEI 330
A + G LT D+ G A T+E T+ II +++ + PF + ++
Sbjct: 318 NALLYTLEEGRVLTGDVVGYDRGAKTTEYTEAIIQNLGKTPRKTQVRGYKPFRLPQV 374
>pir||A43934 isocitrate dehydrogenase (NADP+) (EC 1.1.1.42) - Thermus aquaticus
Length = 494
Score = 177 bits (445), Expect = 1e-43
Identities = 123/357 (34%), Positives = 191/357 (53%), Gaps = 34/357 (9%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKR-IGSPISEDDLKEIRKMD 62
+ VI GDGIGPE V++ ++V+ + + + E G VF+R I S + ++ ++ IRK
Sbjct: 21 ITVIPGDGIGPECVEATLKVLEAAKAPLAYEVREAGASVFRRGIASGVPQETIESIRKTR 80
Query: 63 AILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSN--------GKEIVIVRENT 114
+L G TP G +S VTLRK + YAN+R + + N G ++V+VREN
Sbjct: 81 VVLKGPLETPVGY-GEKSANVTLRKLFETYANVRPVREFPNVPTPYAGRGIDLVVVRENV 139
Query: 115 EGLYAR-DGIGFSDRAIDFRIITLEGARRIAKFAINLAK-ERNSFITFVHKANVLK---- 168
E LYA + + A ++I+ +G+ +I +FA LA+ E + K+N++K
Sbjct: 140 EDLYAGIEHMQTPSVAQTLKLISWKGSEKIVRFAFELARAEGRKKVHCATKSNIMKLAEG 199
Query: 169 GDRFFREIVLEIAEREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHA 228
R F ++ E + E V + I+D+ +LVK P VI+T NM GDI+SDL +
Sbjct: 200 PKRAFEQVAQEYPDIEAVHI---IVDNAAHQLVKRPEQFEVIVTTNMNGDILSDLTSGLI 256
Query: 229 GSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLG--LNGKVVW 286
G +G PS N G+E+A+FE +HGSAP AGK + NP +LSA MML YL ++
Sbjct: 257 GGLGFAPSANIGNEVAIFEAVHGSAPKYAGKNVINPTAVLLSAVMMLRYLEEFATADLIE 316
Query: 287 EATRRYVRYGN-LTPDMGG---TATTSEVTKGII---------SEIYSFDPFDIDEI 330
A + G LT D+ G A T+E T+ II +++ + PF + ++
Sbjct: 317 NALLYTLEEGRVLTGDVVGYDRGAKTTEYTEAIIQNLGKTPRKTQVRGYKPFRLPQV 373
>sp|P70792|TTC4_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC' (TDH)
>gi|984373|gb|AAB61628.1| (U32375) tartrate
dehydrogenase [Agrobacterium vitis]
Length = 358
Score = 176 bits (441), Expect = 4e-43
Identities = 124/351 (35%), Positives = 188/351 (53%), Gaps = 35/351 (9%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR-----IRFYEFEGGFEVFKRIGSPISEDDLK 56
Y++A I DGIGPEV+ + ++V+ ++ R I F+ G + +K+ G + D L+
Sbjct: 4 YKIAAIPADGIGPEVIAAGLQVLEALEKRSGDFSIHTETFDWGSDYYKKNGVMMPADGLE 63
Query: 57 EIRKMDAILFGATTTPFNVPGYRSLI---VTLRKELDLYANLR-------IIPDLSN--- 103
+++K DAI FGA P +VP + +L + + + D YAN+R I P L N
Sbjct: 64 QLKKFDAIFFGAVGAP-DVPDHITLWGLRLPICQGFDQYANVRPTKVLPGITPPLRNCGP 122
Query: 104 -GKEIVIVRENTEGLYARDGIGFSDRAI------DFRIITLEGARRIAKFAINLAKER-N 155
+ VIVREN+EG Y+ G G + + + + I T G RI ++A LA+ R
Sbjct: 123 GDLDWVIVRENSEGEYSGHG-GRAHKGLPEEVGTEVAIFTRVGVTRIMRYAFKLAQARPR 181
Query: 156 SFITFVHKANVLK-GDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTE 213
+T V K+N + G + EI E++ E V + ++D+ +++ P I+
Sbjct: 182 KLLTVVTKSNAQRHGMVMWDEIAAEVSKEFPDVTWDKMLVDAMTVRMTLKPQSLDTIVAT 241
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNYGDEI---ALFEPIHGSAPDIAGKGIANPIGAILS 270
N+ DI+SDLA AGS+G+ P+ N E ++FEPIHGSA DI GKGIANP+ +
Sbjct: 242 NLHADILSDLAGALAGSLGVAPTANIDPERRFPSMFEPIHGSAFDITGKGIANPVATFWT 301
Query: 271 AAMMLDYLGLNGKV--VWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
AA ML++LG + A R G LTPD+GGTA T +VT + I
Sbjct: 302 AAQMLEHLGEKDAATRLMSAVERVTEAGILTPDVGGTANTQQVTDAVCEAI 352
>pir||T50386 probable isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) chain 2
precursor, mitochondrial [imported] - fission yeast
(Schizosaccharomyces pombe) >gi|6522995|emb|CAB62099.1|
(AL133306) probable isocitrate dehydrogenase [nad]
subunit 2, mitochondrial precursor(ec 1.1.1.41)
[Schizosaccharomyces pombe]
Length = 378
Score = 176 bits (441), Expect = 4e-43
Identities = 113/338 (33%), Positives = 184/338 (54%), Gaps = 26/338 (7%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKM 61
Y V +I GDGIGPE+ S R+ + I +E + + K + I +D + +RK
Sbjct: 46 YTVTMIAGDGIGPEIAQSVERIFKAAKVPIE-WERVKVYPILKNGTTTIPDDAKESVRKN 104
Query: 62 DAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRENTE 115
L G TP G+ S+ +TLR+ L+AN+R ++ K V++RENTE
Sbjct: 105 KVALKGPLATPIG-KGHVSMNLTLRRTFGLFANVRPCVSITGYKTPYDNVNTVLIRENTE 163
Query: 116 GLYARDGIGFSDRAI-----DFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK- 168
G Y+ G I ++IT + R+ ++A A++ + IT VHKA +++
Sbjct: 164 GEYS----GIEHEVIPGVVQSIKLITRAASERVIRYAFQYARQTGKNNITVVHKATIMRM 219
Query: 169 GDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNP--WDHGVILTENMFGDIISDLAT 225
D F E E+A E +E+RE I+D+ +K+V +P +++ V++ N++GDI+SD+
Sbjct: 220 ADGLFLECAKELAPEYPDIELREEILDNACLKIVTDPVPYNNTVMVMPNLYGDIVSDMCA 279
Query: 226 IHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLNGKVV 285
G +G+ PSGN G++ ++FE +HG+APDIAGKG+ANP +LS+ MML ++ LN
Sbjct: 280 GLIGGLGLTPSGNIGNQASIFEAVHGTAPDIAGKGLANPTALLLSSVMMLKHMNLNDYAK 339
Query: 286 WEATRRYVRYGN----LTPDMGGTATTSEVTKGIISEI 319
+ + N T D+GG + + T IIS++
Sbjct: 340 RIESAIFDTLANNPDARTKDLGGKSNNVQYTDAIISKL 377
>sp|P70787|TTC2_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC (TDH)
>gi|984368|gb|AAB61623.1| (U32375) tartrate
dehydrogenase [Agrobacterium vitis]
Length = 364
Score = 175 bits (440), Expect = 5e-43
Identities = 124/351 (35%), Positives = 188/351 (53%), Gaps = 35/351 (9%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR-----IRFYEFEGGFEVFKRIGSPISEDDLK 56
Y++A I DGIGPEV+ + ++V+ ++ R I F+ G + +K+ G + D L+
Sbjct: 4 YKIAAIPADGIGPEVIAAGLQVLEALEKRSGDFSIHTETFDWGSDYYKKNGVMMPADGLE 63
Query: 57 EIRKMDAILFGATTTPFNVPGYRSLI---VTLRKELDLYANLR-------IIPDLSN--- 103
+++K DAI FGA P +VP + +L + + + D YAN+R I P L N
Sbjct: 64 QLKKFDAIFFGAVGAP-DVPDHITLWGLRLPICQGFDQYANVRPTKVLPGITPPLRNCGP 122
Query: 104 -GKEIVIVRENTEGLYARDGIGFSDRAI------DFRIITLEGARRIAKFAINLAKER-N 155
+ VIVREN+EG Y+ G G + + + + I T G RI ++A LA+ R
Sbjct: 123 GDLDWVIVRENSEGEYSGHG-GRAHKGLPEEVGTEVAIFTRVGVTRIMRYAFKLAQARPR 181
Query: 156 SFITFVHKANVLK-GDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTE 213
+T V K+N + G + EI E++ E V + ++D+ +++ P I+
Sbjct: 182 KLLTVVTKSNAQRHGMVMWDEIAAEVSKEFPDVTWDKMLVDAMTVRMTLKPQSLDTIVAT 241
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNYGDEI---ALFEPIHGSAPDIAGKGIANPIGAILS 270
N+ DI+SDLA AGS+G+ P+ N E ++FEPIHGSA DI GKGIANP+ +
Sbjct: 242 NLHADILSDLAGALAGSLGVAPTANIDPERRFPSMFEPIHGSAFDITGKGIANPVATFWT 301
Query: 271 AAMMLDYLGLNGKV--VWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
AA ML++LG + A R G LTPD+GGTA T +VT + I
Sbjct: 302 AAQMLEHLGEKDAATRLMSAVERVTEAGILTPDVGGTADTQQVTDAVCEAI 352
>pir||S30897 3-isopropylmalate dehydrogenase (EC 1.1.1.85) precursor - potato
Length = 357
Score = 175 bits (439), Expect = 7e-43
Identities = 113/298 (37%), Positives = 169/298 (55%), Gaps = 20/298 (6%)
Query: 1 MYRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRK 60
+ R + GDGIGPE+ +S ++ I + E G EV R S ++ + L+ +R+
Sbjct: 36 LIRATLFPGDGIGPEIAESVRQIFKVAEVPIEWEEHYVGTEVDPRTNSFLTWESLESVRR 95
Query: 61 MDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRENT 114
L G TP G+RSL +TLRKEL+LYAN+R L K ++ +RENT
Sbjct: 96 NKVGLKGPMATPIG-KGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENT 154
Query: 115 EGLYARDGIGFSDRAI-----DFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVL- 167
EG Y+ G + + +IIT + + R+A++A + AK ++ +HKAN++
Sbjct: 155 EGEYS----GLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQ 210
Query: 168 KGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATI 226
K D F + E+AE+ ++ E +ID+ M+ LVKNP V++ N++GDIISDL
Sbjct: 211 KTDGLFLKCCREVAEKYPEIKYEEVVIDNCMMMLVKNPALFDVLVMPNLYGDIISDLCAG 270
Query: 227 HAGSIGIVPSGNYGD-EIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLNGK 283
G +G+ PS N G+ IAL E +HGSAPDIAGK +ANP +LS+ ML +L L+ K
Sbjct: 271 LIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVSMLRHLELHDK 328
>sp|P94929|LEU3_MYCBO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|1813642|gb|AAC45173.1| (U78886)
3-isopropylmalate dehydrogenase [Mycobacterium bovis]
Length = 336
Score = 175 bits (438), Expect = 9e-43
Identities = 114/299 (38%), Positives = 168/299 (56%), Gaps = 23/299 (7%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKMD 62
++A+I GDGIGPEV A++V+++V ++ ++ G F G + + + E+R D
Sbjct: 2 KLAIIAGDGIGPEVTAEAVKVLDAVVPGVQKTSYDLGARRFHATGEVLPDSVVAELRNHD 61
Query: 63 AILFGATTTPFNVPGY--RSLIVTLRKELDLYANLR---IIPDLSN------GKEIVIVR 111
AIL GA P G R L++ LR ELD + NLR + P +++ G + V+VR
Sbjct: 62 AILLGAIGDPSVPSGVLERGLLLRLRFELDHHINLRPARLYPGVASPLSGNPGIDFVVVR 121
Query: 112 ENTEGLYARDGIGF-----SDRAIDFRIITLEGARRIAKFAINLAKERNSFITFVHKANV 166
E TEG Y +G ++ A + + T G RR+ A A+ R +T VHK NV
Sbjct: 122 EGTEGPYTGNGGAIRVGTPNEVATEVSVNTAFGVRRVVADAFERARRRRKHLTLVHKTNV 181
Query: 167 LK--GDRFFREIVLEIAE-REGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDL 223
L G + R + E+ E VEV +D+ I ++ +P VI+T+N+FGDII+DL
Sbjct: 182 LTLAGGLWLRTVD-EVGECYPDVEVAYQHVDAATIHMITDPGRFDVIVTDNLFGDIITDL 240
Query: 224 ATIHAGSIGIVPSGNYGDEIA---LFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLG 279
A G IG+ SGN A +FEP+HGSAPDIAG+GIA+P AI+S A++L +LG
Sbjct: 241 AAAVCGGIGLAASGNIDATRANPSMFEPVHGSAPDIAGQGIADPTAAIMSVALLLSHLG 299
>sp|P95313|LEU3_MYCTU 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|7431335|pir||F70854 probable leuB protein
- Mycobacterium tuberculosis (strain H37RV)
>gi|1813644|gb|AAC45174.1| (U78887) 3-isopropylmalate
dehydrogenase [Mycobacterium tuberculosis]
>gi|2791592|emb|CAA16080.1| (AL021287) leuB
[Mycobacterium tuberculosis]
Length = 336
Score = 175 bits (438), Expect = 9e-43
Identities = 114/299 (38%), Positives = 168/299 (56%), Gaps = 23/299 (7%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKMD 62
++A+I GDGIGPEV A++V+++V ++ ++ G F G + + + E+R D
Sbjct: 2 KLAIIAGDGIGPEVTAEAVKVLDAVVPGVQKTSYDLGARRFHATGEVLPDSVVAELRNHD 61
Query: 63 AILFGATTTPFNVPGY--RSLIVTLRKELDLYANLR---IIPDLSN------GKEIVIVR 111
AIL GA P G R L++ LR ELD + NLR + P +++ G + V+VR
Sbjct: 62 AILLGAIGDPSVPSGVLERGLLLRLRFELDHHINLRPARLYPGVASPLSGNPGIDFVVVR 121
Query: 112 ENTEGLYARDGIGF-----SDRAIDFRIITLEGARRIAKFAINLAKERNSFITFVHKANV 166
E TEG Y +G ++ A + + T G RR+ A A+ R +T VHK NV
Sbjct: 122 EGTEGPYTGNGGAIRVGTPNEVATEVSVNTAFGVRRVVADAFERARRRRKHLTLVHKTNV 181
Query: 167 LK--GDRFFREIVLEIAE-REGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDL 223
L G + R + E+ E VEV +D+ I ++ +P VI+T+N+FGDII+DL
Sbjct: 182 LTFAGGLWLRTVD-EVGECYPDVEVAYQHVDAATIHMITDPGRFDVIVTDNLFGDIITDL 240
Query: 224 ATIHAGSIGIVPSGNYGDEIA---LFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLG 279
A G IG+ SGN A +FEP+HGSAPDIAG+GIA+P AI+S A++L +LG
Sbjct: 241 AAAVCGGIGLAASGNIDATRANPSMFEPVHGSAPDIAGQGIADPTAAIMSVALLLSHLG 299
>sp|O33117|LEU3_MYCLE 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|11251545|pir||T45419 3-isopropylmalate
dehydrogenase [imported] - Mycobacterium leprae
>gi|2414552|emb|CAB16441.1| (Z99263) 3-isopropylmalate
dehydrogenase [Mycobacterium leprae]
Length = 336
Score = 174 bits (436), Expect = 2e-42
Identities = 117/298 (39%), Positives = 169/298 (56%), Gaps = 21/298 (7%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKMD 62
++A+I GDGIGPEVV A+++++ V ++ ++ G + G + E L E+R+ D
Sbjct: 2 KLAIIGGDGIGPEVVAQAVKILDVVLPGVQKTTYDLGARRYHTTGELLPESVLAELREHD 61
Query: 63 AILFGATTTPFNVPGY--RSLIVTLRKELDLYANLR---IIPDLSN---GK---EIVIVR 111
AIL GA P G R L++ LR ELD + NLR + P +++ GK + V+VR
Sbjct: 62 AILLGAVGDPSVPSGVLERGLLLRLRFELDHHINLRPARLYPGVNSLLAGKPDIDFVVVR 121
Query: 112 ENTEGLYARDGIGF-----SDRAIDFRIITLEGARRIAKFAINLAKERNSFITFVHKANV 166
E TEG Y G ++ A + + T G RR+ + A A++R +T VHK NV
Sbjct: 122 EGTEGPYTGTGGAIRVGTPNEVATEVSVNTAFGVRRVVQDAFERARQRRKHLTLVHKNNV 181
Query: 167 LK-GDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLA 224
L + IV E+ E+ VEV ID+ I LV P VI+T+N+FGDII+DLA
Sbjct: 182 LTYAGTLWCRIVQEVGEKYPDVEVVYQHIDAATIYLVTEPSRFDVIVTDNLFGDIITDLA 241
Query: 225 TIHAGSIGIVPSGNYG---DEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLG 279
G I + SGN ++FEP+HGSAPDIAG+GIA+P AI+S A++L +LG
Sbjct: 242 AAVCGGIALAASGNIDATRTNPSMFEPVHGSAPDIAGQGIADPTAAIMSLALLLAHLG 299
>gb|AAF48965.1| (AE003512) CG12233 gene product [Drosophila melanogaster]
Length = 377
Score = 173 bits (435), Expect = 2e-42
Identities = 119/342 (34%), Positives = 183/342 (52%), Gaps = 38/342 (11%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFE--------GGFEVFKRIGSPISEDD 54
+V +I GDGIGPE+ + ++ + I + + G F G P + D
Sbjct: 49 KVTLIPGDGIGPEISAAVQKIFTAANVPIEWEAVDVTPVRGPDGKF------GIPQAAID 102
Query: 55 LKEIRKMDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIV 108
K+ L G TP G+RSL + LRKE +LYAN+R L K ++V
Sbjct: 103 SVNTNKIG--LKGPLMTPVG-KGHRSLNLALRKEFNLYANVRPCRSLEGYKTLYDDVDVV 159
Query: 109 IVRENTEGLYARDGIGFSDRAID-----FRIITLEGARRIAKFAINLAKERN-SFITFVH 162
+RENTEG Y+ G +D ++IT E ++R+A++A AK N +T VH
Sbjct: 160 TIRENTEGEYS----GIEHEIVDGVVQSIKLITEEASKRVAEYAFQYAKNNNRKKVTVVH 215
Query: 163 KANVLK-GDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDII 220
KAN+++ D F V ++A++ ++ E +D+ + +V+NP + V++ N++GDI+
Sbjct: 216 KANIMRMSDGLFLRCVRDMAQKFPEIQFEEKYLDTVCLNMVQNPGKYDVLVMPNLYGDIL 275
Query: 221 SDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGL 280
SD+ G +G+ PSGN G ALFE +HG+APDIAGK +ANP +LSA MML ++ L
Sbjct: 276 SDMCAGLVGGLGLTPSGNMGLNGALFESVHGTAPDIAGKDLANPTALLLSAVMMLRHMEL 335
Query: 281 N--GKVVWEATRRYVRYGN-LTPDMGGTATTSEVTKGIISEI 319
N + A ++ G LT D+GG A SE T I +++
Sbjct: 336 NTYADKIERAAFETIKEGKYLTGDLGGRAKCSEFTNEICAKL 377
>sp|P29696|LEU3_SOLTU 3-ISOPROPYLMALATE DEHYDROGENASE PRECURSOR (BETA-IPM DEHYDROGENASE)
(IMDH) (3-IPM-DH) >gi|82259|pir||S25670
3-isopropylmalate dehydrogenase (EC 1.1.1.85) precursor
- potato >gi|22643|emb|CAA47720.1| (X67310)
3-isopropylmalate dehydrogenase [Solanum tuberosum]
>gi|445064|prf||1908380A beta isopropylmalate
dehydrogenase [Solanum tuberosum]
Length = 357
Score = 173 bits (433), Expect = 4e-42
Identities = 112/298 (37%), Positives = 168/298 (55%), Gaps = 20/298 (6%)
Query: 1 MYRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRK 60
+ R + GDGIGPE+ +S ++ I + E G EV R S ++ + L+ +R+
Sbjct: 36 LIRATLFPGDGIGPEIAESVRQIFKVAEVPIEWEEHYVGTEVDPRTNSFLTWESLESVRR 95
Query: 61 MDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRENT 114
L G TP G+RSL +TLRKEL+LYAN+R L K ++ +RENT
Sbjct: 96 NKVGLKGPMATPIG-KGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENT 154
Query: 115 EGLYARDGIGFSDRAI-----DFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVL- 167
EG Y+ G + + +IIT + + R+A++A + AK ++ +HKAN++
Sbjct: 155 EGEYS----GLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQ 210
Query: 168 KGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATI 226
K D F + E+AE+ ++ E +ID+ + LVKNP V++ N++GDIISDL
Sbjct: 211 KTDGLFLKCCREVAEKYPEIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAG 270
Query: 227 HAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLNGK 283
G +G+ PS N G+ IAL E +HGSAPDIAGK +ANP +LS+ ML +L L+ K
Sbjct: 271 LIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVSMLRHLELHDK 328
>dbj|BAB04789.1| (AP001510) tartrate dehydrogenase [Bacillus halodurans]
Length = 353
Score = 172 bits (432), Expect = 5e-42
Identities = 115/346 (33%), Positives = 181/346 (52%), Gaps = 28/346 (8%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTD-----RIRFYEFEGGFEVFKRIGSPISEDDLKEI 58
++VI GDG+G E++ A+RV+++V + E+ E + G + ED L ++
Sbjct: 6 LSVIPGDGVGKEIMPHALRVLDAVAEVHGGLTFEKVEYPWSCEYYLEHGEMMPEDGLVQL 65
Query: 59 RKMDAILFGATTTPFNVPGYRSL---IVTLRKELDLYANLR-------IIPDL--SNGKE 106
+ DA+ GA P VP + SL ++ +R+ N+R I P + +N +
Sbjct: 66 TESDAVFLGAVGNPQLVPDHVSLWGLLIAIRRGFQQSLNIRHAKQIRGIDPPIKHANQFD 125
Query: 107 IVIVRENTEGLYARDG----IGFSDRAIDFRIITLEGARRIAKFAINLAKERNSFITFVH 162
+++VREN+EG Y+ G G + + I T + +R +A LA R +T
Sbjct: 126 LLVVRENSEGEYSEVGGRIHQGDDEVVVQNAIFTKKATKRAIDYAFQLAATRGKRVTSAT 185
Query: 163 KAN-VLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDII 220
K+N ++ F+ + E+ ++ +E + ID+ LV P + VI+ N+FGDI+
Sbjct: 186 KSNGIVFSMPFWDNVFQEVGKQYPDIEQQSVHIDALTAFLVTKPAEFDVIVASNLFGDIL 245
Query: 221 SDLATIHAGSIGIVPSGNY---GDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDY 277
+D++ GSIGI P+ N G ++FEP+HGSAPDI GKG+ANPIG I + MMLD+
Sbjct: 246 TDVSAAIMGSIGIGPAANLNVEGTYPSMFEPVHGSAPDIVGKGMANPIGQIWTGKMMLDH 305
Query: 278 LGLN--GKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEIYS 321
G GK + + G TPD+GG ATT EV II + S
Sbjct: 306 FGEEELGKTLLDIVEDVTEAGIKTPDIGGRATTGEVADAIIERVKS 351
>sp|P37412|LEU3_SALTY 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|5107574|pdb|1CNZ|A Chain A,
3-Isopropylmalate Dehydrogenase (Ipmdh) From Salmonella
Typhimurium >gi|5107575|pdb|1CNZ|B Chain B,
3-Isopropylmalate Dehydrogenase (Ipmdh) From Salmonella
Typhimurium >gi|732459|gb|AAB60185.1| (U20795)
3-isopropylmalate dehydrogenase [Salmonella typhimurium]
Length = 363
Score = 172 bits (431), Expect = 6e-42
Identities = 123/352 (34%), Positives = 191/352 (53%), Gaps = 36/352 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
Y +AV+ GDGIGPEV+ A++V+++V R I ++ G G P+ + ++
Sbjct: 5 YHIAVLPGDGIGPEVMAQALKVMDAVRSRFDMRITTSHYDVGGIAIDNHGHPLPKATVEG 64
Query: 58 IRKMDAILFGATTTPF--NVPGY----RSLIVTLRKELDLYANLR-------------II 98
+ DAILFG+ P N+P R ++ LRK L++NLR +
Sbjct: 65 CEQADAILFGSVGGPKWENLPPESQPERGALLPLRKHFKLFSNLRPAKLYQGLEAFCPLR 124
Query: 99 PDLS-NGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAK 152
D++ NG +I+ VRE T G+Y R+G G ++A D + RIA+ A A+
Sbjct: 125 ADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEKAFDTEVYHRFEIERIARIAFESAR 184
Query: 153 ERNSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVIL 211
+R +T + KANVL+ +REIV ++A+ VE+ ID+ ++L+K+P V+L
Sbjct: 185 KRRRKVTSIDKANVLQSSILWREIVNDVAKTYPDVELAHMYIDNATMQLIKDPSQFDVLL 244
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILS 270
N+FGDI+SD + GS+G++PS + ++ L+EP GSAPDIAGK IANPI ILS
Sbjct: 245 CSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILS 304
Query: 271 AAMMLDY-LGLN--GKVVWEATRRYVRYGNLTPDM--GGTATTSEVTKGIIS 317
A++L Y L N + +A R + G T D+ G A +++ II+
Sbjct: 305 LALLLRYSLDANDAATAIEQAINRALEEGVRTGDLARGAAAVSTDEMGDIIA 356
>sp|P30125|LEU3_ECOLI 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|628651|pir||S41197 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Escherichia coli
>gi|5542195|pdb|1CM7|A Chain A, 3-Isopropylmalate
Dehydrogenase From Escherichia Coli
>gi|5542196|pdb|1CM7|B Chain B, 3-Isopropylmalate
Dehydrogenase From Escherichia Coli
>gi|216491|dbj|BAA01342.1| (D10483) 3-isopropylmalate
dehydrogenase homolog(PIR:A24537) [Escherichia coli]
>gi|457440|dbj|BAA04537.1| (D17631) 3-isopropylmalate
dehydrogenase [Escherichia coli]
Length = 363
Score = 171 bits (430), Expect = 8e-42
Identities = 112/307 (36%), Positives = 170/307 (54%), Gaps = 31/307 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
Y +AV+ GDGIGPEV+ A++V+++V +R I ++ G G P+ ++
Sbjct: 5 YHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYDVGGAAIDNHGQPLPPATVEG 64
Query: 58 IRKMDAILFGATTTPF------NVPGYRSLIVTLRKELDLYANLR-------------II 98
+ DA+LFG+ P + R ++ LRK L++NLR +
Sbjct: 65 CEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHFKLFSNLRPAKLYQGLEAFCPLR 124
Query: 99 PDLS-NGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAK 152
D++ NG +I+ VRE T G+Y R+G G ++A D + RIA+ A A+
Sbjct: 125 ADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEKAFDTEVYHRFEIERIARIAFESAR 184
Query: 153 ERNSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVIL 211
+R +T + KANVL+ +REIV EIA E VE+ ID+ ++L+K+P V+L
Sbjct: 185 KRRHKVTSIDKANVLQSSILWREIVNEIATEYPDVELAHMYIDNATMQLIKDPSQFDVLL 244
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILS 270
N+FGDI+SD + GS+G++PS + ++ L+EP GSAPDIAGK IANPI ILS
Sbjct: 245 CSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILS 304
Query: 271 AAMMLDY 277
A++L Y
Sbjct: 305 LALLLRY 311
>pir||A64729 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Escherichia coli
>gi|1786260|gb|AAC73184.1| (AE000117) 3-isopropylmalate
dehydrogenase [Escherichia coli K12]
Length = 364
Score = 171 bits (430), Expect = 8e-42
Identities = 112/307 (36%), Positives = 170/307 (54%), Gaps = 31/307 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
Y +AV+ GDGIGPEV+ A++V+++V +R I ++ G G P+ ++
Sbjct: 6 YHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYDVGGAAIDNHGQPLPPATVEG 65
Query: 58 IRKMDAILFGATTTPF------NVPGYRSLIVTLRKELDLYANLR-------------II 98
+ DA+LFG+ P + R ++ LRK L++NLR +
Sbjct: 66 CEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHFKLFSNLRPAKLYQGLEAFCPLR 125
Query: 99 PDLS-NGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAK 152
D++ NG +I+ VRE T G+Y R+G G ++A D + RIA+ A A+
Sbjct: 126 ADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEKAFDTEVYHRFEIERIARIAFESAR 185
Query: 153 ERNSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVIL 211
+R +T + KANVL+ +REIV EIA E VE+ ID+ ++L+K+P V+L
Sbjct: 186 KRRHKVTSIDKANVLQSSILWREIVNEIATEYPDVELAHMYIDNATMQLIKDPSQFDVLL 245
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILS 270
N+FGDI+SD + GS+G++PS + ++ L+EP GSAPDIAGK IANPI ILS
Sbjct: 246 CSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILS 305
Query: 271 AAMMLDY 277
A++L Y
Sbjct: 306 LALLLRY 312
>sp|Q9WZ26|LEU3_THEMA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|7431334|pir||B72363 3-isopropylmalate
dehydrogenase - Thermotoga maritima (strain MSB8)
>gi|4981071|gb|AAD35641.1|AE001730_9 (AE001730)
3-isopropylmalate dehydrogenase [Thermotoga maritima]
Length = 354
Score = 170 bits (427), Expect = 2e-41
Identities = 115/301 (38%), Positives = 168/301 (55%), Gaps = 27/301 (8%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRI--RFYEFEG--GFEVFKRIGSPISEDDLKEI 58
++AV+ GDGIGPEVV A++V+ V + F + G G + R G P+ E+ K
Sbjct: 2 KIAVLPGDGIGPEVVREALKVLEVVEKKTGKTFEKVFGHIGGDAIDRFGEPLPEETKKIC 61
Query: 59 RKMDAILFGATTTPF--NVPGYRSL----IVTLRKELDLYANLRIIPD------------ 100
+ DAI G+ P ++P + ++ LRK L+LYAN+R I
Sbjct: 62 LEADAIFLGSVGGPKWDDLPPEKRPEIGGLLALRKMLNLYANIRPIKVYRSLVHVSPLKE 121
Query: 101 --LSNGKEIVIVRENTEGLYARDGIGFSD-RAIDFRIITLEGARRIAKFAINLAKERNSF 157
+ +G ++V VRE + G+Y G + + D I + RIA+ A +AK R
Sbjct: 122 KVIGSGVDLVTVRELSYGVYYGQPRGLDEEKGFDTMIYDRKTVERIARTAFEIAKNRRKK 181
Query: 158 ITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTENMF 216
+T V KANVL +R++V E+A E VE+ +D+ ++L+ P VILT NMF
Sbjct: 182 VTSVDKANVLYSSMLWRKVVNEVAREYPDVELTHIYVDNAAMQLILKPSQFDVILTTNMF 241
Query: 217 GDIISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLD 276
GDI+SD + GS+G++PS ++GD+ L+EP GSAPDIAGK IANPI ILS AMML+
Sbjct: 242 GDILSDESAALPGSLGLLPSASFGDK-NLYEPAGGSAPDIAGKNIANPIAQILSLAMMLE 300
Query: 277 Y 277
+
Sbjct: 301 H 301
>pir||S20606 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Salmonella
typhimurium >gi|48753|emb|CAA37456.1| (X53376) leuB
[Salmonella typhimurium]
Length = 359
Score = 169 bits (425), Expect = 3e-41
Identities = 120/350 (34%), Positives = 183/350 (52%), Gaps = 31/350 (8%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYE--FEGGFEVFKRIGSPISEDDLKEIR 59
Y +AV+ GDGIGPEV+ A++V+++V I Y ++ G G P+ + ++
Sbjct: 5 YHIAVLPGDGIGPEVMAQALKVMDAVRVLICVYHSHYDVGGIAIDSSGIPLPKATVEGCE 64
Query: 60 KMDAILFGATTTPF------NVPGYRSLIVTLRKELDLYANLR-------------IIPD 100
+ DAILFG+ P P ++ LRK L++NLR + D
Sbjct: 65 QADAILFGSVGGPKMGKFAPGKPAGARRLLPLRKHFKLFSNLRPAKLYQGLEAFCPLRAD 124
Query: 101 LS-NGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKER 154
++ NG +I+ VRE T G+Y R+G G ++A D + RIA+ A A++R
Sbjct: 125 IAANGFDILCVRELTGGIYFGQPKGREGSGQYEKAFDTEVYHRFEIERIARIAFESARKR 184
Query: 155 NSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTE 213
+T + KANVL+ +REIV ++A+ VE+ ID+ ++L+K+P V+L
Sbjct: 185 RCKVTSIDKANVLQSSILWREIVNDVAKTYPDVELAHMYIDNATMQLIKDPSQFDVLLCS 244
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILSAA 272
N+FGDI+SD + GS+G++PS + ++ L+EP GSAPDIAGK IANPI ILS A
Sbjct: 245 NLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILSLA 304
Query: 273 MMLDYLGLN--GKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEIY 320
++L N + +A R + G T D+ A S G I Y
Sbjct: 305 LLLRTADANDAATAIEQAINRALEEGVRTGDLARGAAVSTDEMGDIIARY 354
>sp|P34738|LEU3_NEUCR 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|11251549|pir||T46607 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) beta [imported] - Neurospora
crassa >gi|4204106|gb|AAD10616.1| (U01061)
beta-isopropylmalate dehydrogenase [Neurospora crassa]
Length = 368
Score = 169 bits (424), Expect = 4e-41
Identities = 122/361 (33%), Positives = 189/361 (51%), Gaps = 45/361 (12%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSV-TDRIRFYEFE------GGFEVFKRIGSPISEDD 54
+ + V GD GPEVV AI+V+ ++ T+ +F GG + K P++++
Sbjct: 4 HNIVVFGGDHCGPEVVLEAIKVLKAIETNSPSACKFNLQNHLLGGASIDKH-NDPLTDEA 62
Query: 55 LKEIRKMDAILFGATTTP----FNVPGYRSLIVTLRKELDLYANLR-------------- 96
L + DA+L GA P + ++ LRKEL Y NLR
Sbjct: 63 LNAAKAADAVLLGAIGGPEWGTSSTVRPEQGLLKLRKELGTYGNLRPCNFASESLVDSSP 122
Query: 97 IIPDLSNGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLA 151
+ ++ G + ++VRE T G+Y DG G++ + + R+A F + LA
Sbjct: 123 LKAEVCRGTDFIVVRELTGGIYFGDRTEDDGSGYACDTEPYSRAEIVRIARLAGF-LALA 181
Query: 152 KERNSFITFVHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HG 208
K + + + KANVL R +R+ V ++ +E +++ +IDS + LVKNP +G
Sbjct: 182 KNSPAKVWSLDKANVLATSRLWRKTVTDVISKECPQLQLEHQLIDSAAMLLVKNPRALNG 241
Query: 209 VILTENMFGDIISDLATIHAGSIGIVPSGNYGDEI-------ALFEPIHGSAPDIAGKGI 261
V++T N+FGDIISD A++ GSIG++PS ++G ++EPIHGSAPDI+GKGI
Sbjct: 242 VVITSNLFGDIISDEASVIPGSIGLLPSASWGGIPDGKVKCNGIYEPIHGSAPDISGKGI 301
Query: 262 ANPIGAILSAAMMLDY---LGLNGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISE 318
NP+ ILS AMML Y L V A + + G T D+GG ATTS++ +++E
Sbjct: 302 VNPVCTILSVAMMLRYSLNLPKEADAVEAAVKAAIDNGTKTKDLGGNATTSDMGNAVVAE 361
Query: 319 I 319
+
Sbjct: 362 L 362
>prf||2004294A beta isopropylmalate dehydrogenase [Neurospora crassa]
Length = 368
Score = 169 bits (424), Expect = 4e-41
Identities = 122/361 (33%), Positives = 189/361 (51%), Gaps = 45/361 (12%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSV-TDRIRFYEFE------GGFEVFKRIGSPISEDD 54
+ + V G+ GPEVV AI+V+ ++ T+ +F GG + K P++++
Sbjct: 4 HNIVVFGGNHCGPEVVLEAIKVLKAIETNSPSACKFNLQNHLLGGASIDKH-NDPLTDEA 62
Query: 55 LKEIRKMDAILFGATTTP----FNVPGYRSLIVTLRKELDLYANLR-------------- 96
L + DA+L GA P + ++ LRKEL Y NLR
Sbjct: 63 LNAAKAADAVLLGAIGGPEWGTSSTVRPEQGLLKLRKELGTYGNLRPCNFASESLVDSSP 122
Query: 97 IIPDLSNGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLA 151
+ ++ G + ++VRE T G+Y DG G++ + + R+A F + LA
Sbjct: 123 LKAEVCRGTDFIVVRELTGGIYFGDRTEDDGSGYACDTEPYSRAEIVRIARLAGF-LALA 181
Query: 152 KERNSFITFVHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HG 208
K + + + KANVL R +R+ V ++ +E +++ +IDS + LVKNP +G
Sbjct: 182 KNPPAKVWSLDKANVLATSRLWRKTVTDVISKEFPQLQLEHQLIDSAAMLLVKNPRALNG 241
Query: 209 VILTENMFGDIISDLATIHAGSIGIVPSGNYGDEI-------ALFEPIHGSAPDIAGKGI 261
V++T N+FGDIISD A++ GSIG++PS + G ++EPIHGSAPDI+GKGI
Sbjct: 242 VVITSNLFGDIISDEASVIPGSIGLLPSASLGGIPDGKGKCNGIYEPIHGSAPDISGKGI 301
Query: 262 ANPIGAILSAAMMLDY---LGLNGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISE 318
NP+G ILS AMML Y L V A + + G T D+GG ATTS++ +++E
Sbjct: 302 VNPVGTILSVAMMLRYSLNLPKEADAVEAAVKAAIDNGTKTKDLGGNATTSDMGNAVVAE 361
Query: 319 I 319
+
Sbjct: 362 L 362
>sp|P76251|TTUC_ECOLI PROBABLE TARTRATE DEHYDROGENASE (TDH) >gi|7431321|pir||H64940
tartrate dehydrogenase (EC 1.1.1.93) - Escherichia coli
(strain K-12) >gi|1736423|dbj|BAA15595.1| (D90823)
3-isopropylmalate dehydrogenase (EC 1.1.1.85) (b-IPM
dehydrogenase) (ImdH) (3-IPM-DH). [Escherichia coli]
>gi|1736433|dbj|BAA15604.1| (D90824) 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) (b-IPM dehydrogenase) (ImdH)
(3-IPM-DH). [Escherichia coli]
>gi|1788101|gb|AAC74870.1| (AE000274) putative tartrate
dehydrogenase [Escherichia coli K12]
Length = 361
Score = 169 bits (424), Expect = 4e-41
Identities = 126/351 (35%), Positives = 175/351 (48%), Gaps = 39/351 (11%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFE-GGFEVFKRIGSPISEDDLKE 57
R+A I GDGIG EV+ IRV+ + +R + F + E E + G + +D ++
Sbjct: 6 RIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQMEWASCEYYSHHGKMMPDDWHEQ 65
Query: 58 IRKMDAILFGATTTPFNVPGYRSL---IVTLRKELDLYANLRII------PDLSNGKE-- 106
+ + DAI FGA P VP + SL ++ R+E D Y NLR + P GK+
Sbjct: 66 LSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLAGKQPG 125
Query: 107 ---IVIVRENTEGLYARDGIGFSDRA-----IDFRIITLEGARRIAKFAINLAKER-NSF 157
+VRENTEG Y+ G ++ I + T G RI ++A LA+ R
Sbjct: 126 DIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQSRPRKT 185
Query: 158 ITFVHKANVLKGDRFFREIVLEIAEREGVEVR--EAIIDSFMIKLVKNPWDHGVILTENM 215
+T K+N L + + +E E+R + ID + V P V++ N+
Sbjct: 186 LTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVVVASNL 245
Query: 216 FGDIISDLATIHAGSIGIVPSGNYGDEI---ALFEPIHGSAPDIAGKGIANPIGAILSAA 272
FGDI+SDL G+IGI PS N E +LFEP+HGSAPDI GK IANPI I + A
Sbjct: 246 FGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIATIWAGA 305
Query: 273 MMLDYLGLNG--------KVVWEATRRYVRYGNLTPDMGGTATTSEVTKGI 315
MMLD+LG NG + A + +G TPDM G ATT +V I
Sbjct: 306 MMLDFLG-NGDERFQQAHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAI 355
>pir||D83255 3-isopropylmalate dehydrogenase PA3118 [imported] - Pseudomonas
aeruginosa (strain PAO1) >gi|3237310|gb|AAC23938.1|
(U93274) LeuB [Pseudomonas aeruginosa]
>gi|9949230|gb|AAG06506.1|AE004736_3 (AE004736)
3-isopropylmalate dehydrogenase [Pseudomonas aeruginosa]
Length = 360
Score = 169 bits (424), Expect = 4e-41
Identities = 113/321 (35%), Positives = 178/321 (55%), Gaps = 62/321 (19%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRI---------GSPISED 53
++ V+ GDGIGPE++ A++V+ DR F+ GFE+ + + G P+++
Sbjct: 4 QILVLPGDGIGPEIMAEAVKVLELANDR-----FQLGFELAEDVIGGAAIDKHGVPLADQ 58
Query: 54 DLKEIRKMDAILFGATTTPF------NVPGYRSLIVTLRKELDLYANLR----------- 96
L+ R+ DA+L GA P ++ R L+ +R +L L+ANLR
Sbjct: 59 TLQRARQADAVLLGAVGGPKWDRIERDIRPERGLL-KIRSQLGLFANLRPAILYPQLAAA 117
Query: 97 --IIPDLSNGKEIVIVRENTEGLY----------------ARDGIGFSDRAIDFRIITLE 138
+ P++ G +I+IVRE T G+Y A D + +S+ I
Sbjct: 118 SSLKPEVVAGLDILIVRELTGGIYFGQPREQRVLENGERQAYDTLPYSESEI-------- 169
Query: 139 GARRIAKFAINLAKERNSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFM 197
RRIA+ ++A+ RN+ + V KANVL + +RE+V E+A + VE+ +D+
Sbjct: 170 --RRIARVGFDMARVRNNRLCSVDKANVLASSQLWREVVEEVAKDYPDVELSHMYVDNAA 227
Query: 198 IKLVKNPWDHGVILTENMFGDIISDLATIHAGSIGIVPSGNY-GDEIALFEPIHGSAPDI 256
++LV+ P V++T+NMFGDI+SD A++ GSIG++PS + + ++EP HGSAPDI
Sbjct: 228 MQLVRAPKQFDVMVTDNMFGDILSDEASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDI 287
Query: 257 AGKGIANPIGAILSAAMMLDY 277
AG+GIANP+ ILS +MML Y
Sbjct: 288 AGQGIANPLATILSVSMMLRY 308
>sp|Q01987|LEU3_CANBO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|323016|pir||A43324 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - yeast (Candida boidinii)
>gi|170880|gb|AAA34349.1| (M98832) 3-isopropylmalate
dehydrogenase [Candida boidinii]
Length = 365
Score = 169 bits (423), Expect = 5e-41
Identities = 121/357 (33%), Positives = 189/357 (52%), Gaps = 43/357 (12%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTD-----RIRFYEFEGGFEVFKRIGSPISEDDLKE 57
++ ++ GD +G EVV+ A++++ S+++ + +F G G P+ ++ L+
Sbjct: 7 KIVLLPGDHVGVEVVEEAVKILKSISEVKPEIQFKFENHLIGGAAIDATGVPLPDEALEA 66
Query: 58 IRKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIPD 100
+K DA+L GA P G ++ +RKEL+LYANLR + D
Sbjct: 67 AKKSDAVLLGAVGGPKWGTGEVRPEQGLLKIRKELNLYANLRPCNFASDKLLDLSPLKSD 126
Query: 101 LSNGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN 155
+ G + +VRE G+Y DG GF+ + + + +E R+A F ++L +
Sbjct: 127 IVKGTDFTVVRELVGGIYFGDRVEDDGSGFASDSESYSVPEVERITRMAAF-LSLQNDPP 185
Query: 156 SFITFVHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGVILT 212
I + KANVL R +R+ V + + E + V+ +IDS + LVK+P +G+++T
Sbjct: 186 LPIWSLDKANVLASSRLWRKTVDRVIKEEFPKLTVQHQLIDSAAMILVKSPTKLNGIVIT 245
Query: 213 ENMFGDIISDLATIHAGSIGIVPSGNY------GDEIALFEPIHGSAPDIAGKGIANPIG 266
NMFGDIISD A++ GS+G++PS + L+EP HGSAPD+ K NPI
Sbjct: 246 NNMFGDIISDEASVIPGSLGLLPSASLASLPDTNQAFGLYEPCHGSAPDLP-KNKVNPIA 304
Query: 267 AILSAAMMLDYLGLN----GKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
ILSAAMML L LN G V EA R+ + G +T D+GG +T+EV I E+
Sbjct: 305 TILSAAMMLK-LSLNLVKEGNAVEEAVRKVLDQGIMTGDLGGNNSTTEVGDAIAKEV 360
>sp|Q51945|TTUC_PSEPU TARTRATE DEHYDROGENASE (TDH) >gi|1084041|pir||S48640 tartrate
dehydrogenase (EC 1.1.1.93) - Pseudomonas putida
>gi|458335|gb|AAA60327.1| (U05986) tartrate
dehydrogenase [Pseudomonas putida]
Length = 365
Score = 168 bits (422), Expect = 7e-41
Identities = 128/360 (35%), Positives = 185/360 (50%), Gaps = 38/360 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFE-GGFEVFKRIGSPISEDDLK 56
+R+A I GDGIG EV+ IRV+ + + + F FE + + + G + +D +
Sbjct: 6 FRIAAIPGDGIGLEVLPEGIRVLEAAALKHGLALEFDTFEWASCDYYLQHGKMMPDDWAE 65
Query: 57 EIRKMDAILFGATTTPFNVPGYRSL---IVTLRKELDLYANLR---IIPD----LSNGK- 105
++++ DAI FGA P VP + SL ++ R+E D Y N+R + P L+N K
Sbjct: 66 QLKQYDAIYFGAVDWPDKVPDHISLWGSLLKFRREFDQYVNIRPVRLFPGVPCALANRKV 125
Query: 106 ---EIVIVRENTEGLYAR-DGIGFSDR----AIDFRIITLEGARRIAKFAINLAKERN-S 156
+ V+VRENTEG Y+ GI F + I I T G RI K+A +LA++R
Sbjct: 126 GDIDFVVVRENTEGEYSSLGGIMFENTENEIVIQESIFTRRGVDRILKYAFDLAEKRERK 185
Query: 157 FITFVHKAN--VLKGDRFFREIVLEIAEREGVEVREAIIDSFMIKLVKNPWDHGVILT-E 213
+T K+N + + + A V + ID + V P V++
Sbjct: 186 HVTSATKSNGMAISMPYWDKRTEAMAAHYPHVSWDKQHIDILCARFVLQPERFDVVVVAS 245
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNYGDEI---ALFEPIHGSAPDIAGKGIANPIGAILS 270
N+FGDI+SDL AG+IGI PS N E +LFEP+HGSAPDI GK IANPI I S
Sbjct: 246 NLFGDILSDLGPACAGTIGIAPSANLNPERNFPSLFEPVHGSAPDIFGKNIANPIAMIWS 305
Query: 271 AAMMLDYLGLNGK-------VVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEIYSFD 323
A+ML++LG + + A R + G++TPDMGGT +T +V I + D
Sbjct: 306 GALMLEFLGQGDERYQRAHDDMLNAIERVIADGSVTPDMGGTLSTQQVGAAISDTLARLD 365
>sp|Q51375|LEU3_PSEAE 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|1314267|gb|AAC45419.1| (U29655)
3-isopropylmalate dehydrogenase [Pseudomonas aeruginosa]
Length = 360
Score = 168 bits (422), Expect = 7e-41
Identities = 113/321 (35%), Positives = 177/321 (54%), Gaps = 62/321 (19%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRI---------GSPISED 53
++ V+ GDGIGPE++ A++V+ DR F+ GFE+ + + G P+++
Sbjct: 4 QILVLPGDGIGPEIMAEAVKVLELANDR-----FQLGFELAEDVIGGAAIDKHGVPLADQ 58
Query: 54 DLKEIRKMDAILFGATTTPF------NVPGYRSLIVTLRKELDLYANLR----------- 96
L+ R+ DA+L GA P ++ R L+ R +L L+ANLR
Sbjct: 59 TLQRARQADAVLLGAVGGPKWDRIERDIRPERGLL-KFRSQLGLFANLRPAILYPQLAAA 117
Query: 97 --IIPDLSNGKEIVIVRENTEGLY----------------ARDGIGFSDRAIDFRIITLE 138
+ P++ G +I+IVRE T G+Y A D + +S+ I
Sbjct: 118 SSLKPEVVAGLDILIVRELTGGIYFGQPREQRVLENGERQAYDTLPYSESEI-------- 169
Query: 139 GARRIAKFAINLAKERNSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFM 197
RRIA+ ++A+ RN+ + V KANVL + +RE+V E+A + VE+ +D+
Sbjct: 170 --RRIARVGFDMARVRNNRLCSVDKANVLASSQLWREVVEEVAKDYPDVELSHMYVDNAA 227
Query: 198 IKLVKNPWDHGVILTENMFGDIISDLATIHAGSIGIVPSGNY-GDEIALFEPIHGSAPDI 256
++LV+ P V++T+NMFGDI+SD A++ GSIG++PS + + ++EP HGSAPDI
Sbjct: 228 MQLVRAPKQFDVMVTDNMFGDILSDEASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDI 287
Query: 257 AGKGIANPIGAILSAAMMLDY 277
AG+GIANP+ ILS +MML Y
Sbjct: 288 AGQGIANPLATILSVSMMLRY 308
>gb|AAB91428.1| (U86815) beta-isopropylmalate dehydrogenase [Expression vector
pESP-2]
Length = 364
Score = 168 bits (421), Expect = 9e-41
Identities = 126/358 (35%), Positives = 192/358 (53%), Gaps = 45/358 (12%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTD---RIRFYEFEG---GFEVFKRIGSPISEDDLK 56
++ V+ GD +G E+ AI+V+ +++D ++F +FE G G P+ ++ L+
Sbjct: 6 KIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKF-DFENHLIGGAAIDATGVPLPDEALE 64
Query: 57 EIRKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIP 99
+K+DA+L GA P G ++ +RKEL LYANLR I P
Sbjct: 65 ASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYANLRPCNFASDSLLDLSPIKP 124
Query: 100 DLSNGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKER 154
+ G + V+VRE G+Y DG G + + + + ++ R+A F + L E
Sbjct: 125 QFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYTVPEVQRITRMAAF-MALQHEP 183
Query: 155 NSFITFVHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGVIL 211
I + KANVL R +R+ V E + E ++V+ +IDS + LVKNP +G+I+
Sbjct: 184 PLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNGIII 243
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGD------EIALFEPIHGSAPDIAGKGIANPI 265
T NMFGDIISD A++ GS+G++PS + L+EP HGSAPD+ K ANPI
Sbjct: 244 TSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLP-KNKANPI 302
Query: 266 GAILSAAMMLDYLGLN----GKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
ILSAAMML L LN GK + +A ++ + G T D+GG+ +T+EV + E+
Sbjct: 303 ATILSAAMMLK-LSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAEEV 359
>sp|P31958|LEU3_CLOPA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|144842|gb|AAC41394.1| (L06666)
isopropylmalate dehydrogenase [Clostridium pasteurianum]
Length = 357
Score = 168 bits (420), Expect = 1e-40
Identities = 108/305 (35%), Positives = 168/305 (54%), Gaps = 29/305 (9%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
+++AVI GDGIGP++V A++++ V ++ F E + G + G P+ ++ +
Sbjct: 4 FKIAVIPGDGIGPDIVREAVKIMTKVGEKYDTKFNFVEVKAGGDAIDAYGEPLPKETIDV 63
Query: 58 IRKMDAILFGATTTPF--NVPGYRS---LIVTLRKELDLYANLR------IIPDLS---- 102
+ A+L GA P N+ G + ++ LR L LYANLR ++ S
Sbjct: 64 CKSSAAVLLGAVGGPKWDNLEGSKRPERALLGLRGALGLYANLRPAKVYNVLKSASPLKN 123
Query: 103 ----NGKEIVIVRENTEGLYARDG----IGFSDRAIDFRIITLEGARRIAKFAINLAKER 154
G ++++VRE G+Y D + + A D ++ +RIA A A +R
Sbjct: 124 EIIDEGVDLLVVRELIGGIYFGDRGTKEVNGVETAFDTEKYNVDEVKRIAHSAFKAAMKR 183
Query: 155 NSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTE 213
+T V KANVL R +R+ V E++ E VE+ +D+ ++LV+ P VILT
Sbjct: 184 RKKVTSVDKANVLDASRLWRKTVNEVSKEYPEVELSHLYVDNTAMQLVRKPSQFDVILTN 243
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNYG-DEIALFEPIHGSAPDIAGKGIANPIGAILSAA 272
N+FGDI+SD A++ GSIG++ S + D ++EPIHGSAPDIAG IANP+ ILSAA
Sbjct: 244 NIFGDILSDEASMITGSIGMLASSSIREDSFGMYEPIHGSAPDIAGLDIANPLAQILSAA 303
Query: 273 MMLDY 277
M+++Y
Sbjct: 304 MLMEY 308
>gb|AAB49959.1| (U70024) LEU2 [Cloning vector pGAD-C1] >gi|1595845|gb|AAB49962.1|
(U70025) LEU2 [Cloning vector pGAD-C2]
>gi|1595849|gb|AAB49965.1| (U70026) LEU2 [Cloning vector
pGAD-C3]
Length = 364
Score = 168 bits (420), Expect = 1e-40
Identities = 125/358 (34%), Positives = 193/358 (52%), Gaps = 45/358 (12%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTD---RIRFYEFEG---GFEVFKRIGSPISEDDLK 56
++ V+ GD +G E+ AI+V+ +++D ++F +FE G F+ G P+ ++ L+
Sbjct: 6 KIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKF-DFENHLIGGAAFEATGVPLPDEALE 64
Query: 57 EIRKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIP 99
+K+DA+L GA P G ++ +RKEL LYANLR I P
Sbjct: 65 ASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYANLRPCNFASDSLLDLSPIKP 124
Query: 100 DLSNGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKER 154
+ G + V+VRE G+Y DG G + + + + ++ R+A F + L E
Sbjct: 125 QFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYTVPEVQRITRMAAF-MALQHEP 183
Query: 155 NSFITFVHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGVIL 211
I + KANVL R +R+ V E + E ++V+ +IDS + LVKNP +G+I+
Sbjct: 184 PLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNGIII 243
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGD------EIALFEPIHGSAPDIAGKGIANPI 265
T NMFGDIISD A++ GS+G++PS + L+EP HGSAPD+ K +PI
Sbjct: 244 TSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLP-KNKVDPI 302
Query: 266 GAILSAAMMLDYLGLN----GKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
ILSAAMML L LN GK + +A ++ + G T D+GG+ +T+EV + E+
Sbjct: 303 ATILSAAMMLK-LSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAEEV 359
>gb|AAC69608.1| (AF045153) NAD-dependent isocitrate dehydrogenase subunit 1
[Kluyveromyces lactis]
Length = 361
Score = 167 bits (418), Expect = 2e-40
Identities = 114/340 (33%), Positives = 185/340 (53%), Gaps = 33/340 (9%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFE-GGFEVFKRIGSPISEDDLKEIRK 60
Y V +I GDG+G EV DS +++ + I + + G E + + + + +++
Sbjct: 30 YTVTLIPGDGVGKEVTDSVVKIFENENIPIDWETIDISGLENTENVQRAV-----ESLKR 84
Query: 61 MDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLS------NGKEIVIVRENT 114
L G TP + G+ SL V LRK+LD++AN+ + + N ++VI+RENT
Sbjct: 85 NKVGLKGIWHTPADQTGHGSLNVALRKQLDIFANVALFKSIPGVKTRLNNIDMVIIRENT 144
Query: 115 EGLYARDGIGFSDRAI-----DFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK 168
EG Y+ G ++ +I+T + RIA+FA + A + N + VHKAN++K
Sbjct: 145 EGEYS----GLEHESVPGVVESLKIMTRAKSERIARFAFDFALKNNRKSVCAVHKANIMK 200
Query: 169 -GDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLAT 225
GD FR V EI E ++V+ I+D+ ++ V P V++T N++G I+ ++ +
Sbjct: 201 LGDGLFRNTVNEIGANEYPELDVKNIIVDNASMQAVAKPHQFDVLVTPNLYGSILGNIGS 260
Query: 226 IHAGSIGIVPSGNYGDEIALFEPIHGS---APDIAGKGIANPIGAILSAAMMLDYLGLNG 282
G G+VP N+G E A+FEP GS DI G+ +ANP ILS+ +ML +LGLN
Sbjct: 261 ALIGGPGLVPGANFGREYAVFEP--GSRHVGLDIKGQNVANPTAMILSSTLMLRHLGLNA 318
Query: 283 KV--VWEATRRYVRYG-NLTPDMGGTATTSEVTKGIISEI 319
+ +AT + G + T D+GG+A+TSE T +I ++
Sbjct: 319 YADRISKATYDVISEGKSTTRDIGGSASTSEFTNAVIEKL 358
>sp|Q9ZDR0|IDH_RICPR ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCCINATE DECARBOXYLASE)
(IDH) (NADP+-SPECIFIC ICDH) (IDP)
>gi|7467879|pir||E71681 isocitrate dehydrogenase (icd)
RP265 - Rickettsia prowazekii
>gi|3860827|emb|CAA14727.1| (AJ235271) ISOCITRATE
DEHYDROGENASE (icd) [Rickettsia prowazekii]
Length = 483
Score = 167 bits (418), Expect = 2e-40
Identities = 120/359 (33%), Positives = 188/359 (51%), Gaps = 35/359 (9%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKR-IGSPISEDDLKEIRKMD 62
+ + GDGIGPE++++ + ++ RI E G +++K+ S ISE+ I++
Sbjct: 7 ITIAYGDGIGPEIMEAVLYILRKAEARISLETIEVGEKLYKKHYTSGISEESWDVIQRTG 66
Query: 63 AILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPD-------LSNGKEIVIVRENTE 115
IL TTP + GY+SL VT+RK L L+AN+R + L + I+REN E
Sbjct: 67 IILKAPITTPQS-GGYKSLNVTIRKTLQLFANIRPVVSFYPFTRTLHPNLNLTIIRENEE 125
Query: 116 GLYARDGIGFS---DRAIDFRIITLEGARRIAKFAINLA-KERNSFITFVHKANVLK-GD 170
LY+ G+ + + ++I+ G ++I ++A A K +T + K N++K D
Sbjct: 126 DLYS--GVEYRQTHNMYESMKLISHTGCKKIIRYAFEYAIKNNRKKVTCLTKDNIMKFSD 183
Query: 171 RFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAG 229
F + EIA E + IID KL P +I+T N++GDIISD+A +G
Sbjct: 184 GIFHRVFNEIAKEYPQINNEHYIIDIGTAKLATKPEIFDIIVTSNLYGDIISDVAAEISG 243
Query: 230 SIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLN--GKVVWE 287
S+G+ S N G A+FE +HGSAPDIAGKGIANP G + +A MML ++G ++
Sbjct: 244 SVGLAGSANIGQHYAMFEAVHGSAPDIAGKGIANPSGLLNAAIMMLVHIGQGDIASLIEN 303
Query: 288 ATRRYVRYGNLTPDMGGTATTSE--VTKGIISEIYSFDPFDIDEIWVEEIRLGRIPLTL 344
A ++ + G T D+ ++S+ TK E+ RLG++P+TL
Sbjct: 304 AWKKTIEDGVHTFDIYNEHSSSKKVCTKEFAVEVIK--------------RLGQLPITL 348
>pir||DEBYI 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - yeast
(Saccharomyces cerevisiae) >gi|4698|emb|CAA27459.1|
(X03840) beta-isopropylmalate dehydrogenase
[Saccharomyces cerevisiae] >gi|1907128|emb|CAA42366.1|
(X59720) YCL018w, len:364 [Saccharomyces cerevisiae]
>gi|2689263|gb|AAB88909.1| (U67875) 3-isopropylmalate
dehydrogenase [Expression vector pESP-I]
Length = 364
Score = 166 bits (417), Expect = 3e-40
Identities = 125/358 (34%), Positives = 191/358 (52%), Gaps = 45/358 (12%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTD---RIRFYEFEG---GFEVFKRIGSPISEDDLK 56
++ V+ GD +G E+ AI+V+ +++D ++F +FE G G P+ ++ L+
Sbjct: 6 KIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKF-DFENHLIGGAAIDATGVPLPDEALE 64
Query: 57 EIRKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIP 99
+K+DA+L GA P G ++ +RKEL LYANLR I P
Sbjct: 65 ASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYANLRPCNFASDSLLDLSPIKP 124
Query: 100 DLSNGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKER 154
+ G + V+VRE G+Y DG G + + + + ++ R+A F + L E
Sbjct: 125 QFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYTVPEVQRITRMAAF-MALQHEP 183
Query: 155 NSFITFVHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGVIL 211
I + KANVL R +R+ V E + E ++V+ +IDS + LVKNP +G+I+
Sbjct: 184 PLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNGIII 243
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGD------EIALFEPIHGSAPDIAGKGIANPI 265
T NMFGDIISD A++ GS+G++PS + L+EP HGSAPD+ K NPI
Sbjct: 244 TSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLP-KNKVNPI 302
Query: 266 GAILSAAMMLDYLGLN----GKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
ILSAAMML L LN GK + +A ++ + G T D+GG+ +T+EV + E+
Sbjct: 303 ATILSAAMMLK-LSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAEEV 359
>emb|CAA60637.1| (X87172) NAD+-isocitrate dehydrogenase, alpha subunit [Macaca
fascicularis]
Length = 340
Score = 166 bits (417), Expect = 3e-40
Identities = 116/339 (34%), Positives = 184/339 (54%), Gaps = 25/339 (7%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSP-----ISEDDLKEI 58
V +I GDGIGPE+ + +++ ++ I++ E I P I + + +
Sbjct: 8 VTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERN-----VTAIQGPGGKWMIPSEAKESM 62
Query: 59 RKMDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRE 112
K L G TP G+ S+ + LRK DLYAN+R + K IV +RE
Sbjct: 63 DKNKMGLKGPLKTPI-AAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRE 121
Query: 113 NTEGLYARDGIGFSDRAID-FRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK-G 169
NTEG Y+ D + ++IT G++RIA+FA A+ + S +T VHKAN+++
Sbjct: 122 NTEGEYSGIEHVIVDGVVQSIKLITEGGSKRIAEFAFEYARNNHRSNVTAVHKANIMRMS 181
Query: 170 DRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHA 228
D F + E+AE + ++ E +D+ + +V++P V++ N++GDI+SDL
Sbjct: 182 DGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLI 241
Query: 229 GSIGIVPSGNYG-DEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVV 285
G +G+ PSGN G + +A+FE +HG+APDIAGK +ANP +LSA MML ++GL + +
Sbjct: 242 GGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARI 301
Query: 286 WEATRRYVRYG-NLTPDMGGTATTSEVTKGIISEIYSFD 323
A ++ G +LT D+GG A S+ T+ I + D
Sbjct: 302 EAACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKDLD 340
>sp|Q28480|IDHA_MACFA ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|2117495|pir||S58664 isocitrate dehydrogenase (NAD+)
(EC 1.1.1.41) alpha chain - crab-eating macaque
(fragment)
Length = 347
Score = 166 bits (417), Expect = 3e-40
Identities = 116/339 (34%), Positives = 184/339 (54%), Gaps = 25/339 (7%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSP-----ISEDDLKEI 58
V +I GDGIGPE+ + +++ ++ I++ E I P I + + +
Sbjct: 15 VTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERN-----VTAIQGPGGKWMIPSEAKESM 69
Query: 59 RKMDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRE 112
K L G TP G+ S+ + LRK DLYAN+R + K IV +RE
Sbjct: 70 DKNKMGLKGPLKTPI-AAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRE 128
Query: 113 NTEGLYARDGIGFSDRAID-FRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK-G 169
NTEG Y+ D + ++IT G++RIA+FA A+ + S +T VHKAN+++
Sbjct: 129 NTEGEYSGIEHVIVDGVVQSIKLITEGGSKRIAEFAFEYARNNHRSNVTAVHKANIMRMS 188
Query: 170 DRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHA 228
D F + E+AE + ++ E +D+ + +V++P V++ N++GDI+SDL
Sbjct: 189 DGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLI 248
Query: 229 GSIGIVPSGNYG-DEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVV 285
G +G+ PSGN G + +A+FE +HG+APDIAGK +ANP +LSA MML ++GL + +
Sbjct: 249 GGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARI 308
Query: 286 WEATRRYVRYG-NLTPDMGGTATTSEVTKGIISEIYSFD 323
A ++ G +LT D+GG A S+ T+ I + D
Sbjct: 309 EAACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKDLD 347
>gi|10383772 beta-IPM (isopropylmalate) dehydrogenase; Leu2p [Saccharomyces
cerevisiae]
Length = 364
Score = 166 bits (415), Expect = 5e-40
Identities = 125/358 (34%), Positives = 190/358 (52%), Gaps = 45/358 (12%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTD---RIRFYEFEG---GFEVFKRIGSPISEDDLK 56
++ V+ GD +G E+ AI+V+ +++D ++F +FE G G P+ ++ L+
Sbjct: 6 KIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKF-DFENHLIGGAAIDATGVPLPDEALE 64
Query: 57 EIRKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIP 99
+K DA+L GA P G ++ +RKEL LYANLR I P
Sbjct: 65 ASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYANLRPCNFASDSLLDLSPIKP 124
Query: 100 DLSNGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKER 154
+ G + V+VRE G+Y DG G + + + + ++ R+A F + L E
Sbjct: 125 QFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYTVPEVQRITRMAAF-MALQHEP 183
Query: 155 NSFITFVHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGVIL 211
I + KANVL R +R+ V E + E ++V+ +IDS + LVKNP +G+I+
Sbjct: 184 PLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNGIII 243
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGD------EIALFEPIHGSAPDIAGKGIANPI 265
T NMFGDIISD A++ GS+G++PS + L+EP HGSAPD+ K NPI
Sbjct: 244 TSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLP-KNKVNPI 302
Query: 266 GAILSAAMMLDYLGLN----GKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
ILSAAMML L LN GK + +A ++ + G T D+GG+ +T+EV + E+
Sbjct: 303 ATILSAAMMLK-LSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAEEV 359
>sp|P04173|LEU3_YEAST 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|171837|gb|AAA93044.1| (J01333) beta-IPM
dehydrogenase [Saccharomyces cerevisiae]
>gi|682656|gb|AAA74942.1| (L25833) 3-isopropylmalate
dehydrogenase [Cloning vector pYEULCBX]
>gi|915410|gb|AAB39920.1| (U29899) LEU2 [Cloning vector
pACT2] >gi|1000354|gb|AAA76716.1| (U35112) Leu2p
[synthetic construct] >gi|1049257|gb|AAA80348.1|
(U36450) 3-isopropylmalate dehydrogenase [Shuttle vector
pAD-GAL4] >gi|2981189|gb|AAC78773.1| (AF049063)
beta-isopropylmalate dehydrogenase [Expression vector
pYZ1N] >gi|2981194|gb|AAC78777.1| (AF049064)
beta-isopropylmalate dehydrogenase [Expression vector
pYZ3N-GFP] >gi|3136320|gb|AAC78830.1| (AF064066)
beta-isopropylmalate dehydrogenase [Expression vector
pYZ41N] >gi|3136324|gb|AAC78833.1| (AF064067)
beta-isopropylmalate dehydrogenase [Expression vector
pYZ81N] >gi|3243015|gb|AAC23871.1| (AF069719)
isopropylmalate dehydrogenase [Expression vector
pBEVY-GL] >gi|3243025|gb|AAC23878.1| (AF069722)
isopropylmalate dehydrogenase [Expression vector
pBEVY-L] >gi|6642850|gb|AAF20325.1| (AF134167)
isopropylmalate dehydrogenase [Yeast 2-hybrid vector
pC-ACT.1] >gi|6642854|gb|AAF20328.1| (AF134168)
isopropylmalate dehydrogenase [Yeast 2-hybrid vector
pC-ACT.2]
Length = 364
Score = 164 bits (412), Expect = 1e-39
Identities = 124/358 (34%), Positives = 191/358 (52%), Gaps = 45/358 (12%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTD---RIRFYEFEG---GFEVFKRIGSPISEDDLK 56
++ V+ GD +G E+ AI+V+ +++D ++F +FE G G P+ ++ L+
Sbjct: 6 KIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKF-DFENHLIGGAAIDATGVPLPDEALE 64
Query: 57 EIRKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIP 99
+K+DA+L GA P G ++ +RKEL LYANLR I P
Sbjct: 65 ASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYANLRPCNFASDSLLDLSPIKP 124
Query: 100 DLSNGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKER 154
+ G + V+VRE G+Y DG G + + + + ++ R+A F + L E
Sbjct: 125 QFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYTVPEVQRITRMAAF-MALQHEP 183
Query: 155 NSFITFVHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGVIL 211
I + KANVL R +R+ V E + E ++V+ +IDS + LVKNP +G+I+
Sbjct: 184 PLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNGIII 243
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGD------EIALFEPIHGSAPDIAGKGIANPI 265
T NMFGDIISD A++ GS+G++PS + L+EP HGSAPD+ K +PI
Sbjct: 244 TSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLP-KNKVDPI 302
Query: 266 GAILSAAMMLDYLGLN----GKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
ILSAAMML L LN GK + +A ++ + G T D+GG+ +T+EV + E+
Sbjct: 303 ATILSAAMMLK-LSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAEEV 359
>sp|P54354|LEU3_BACFR 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|2117496|pir||I40235 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Bacteroides fragilis
>gi|984575|dbj|BAA08117.1| (D45169) beta-isopropylmalate
dehydrogenase [Bacteroides fragilis]
Length = 353
Score = 164 bits (412), Expect = 1e-39
Identities = 109/348 (31%), Positives = 177/348 (50%), Gaps = 30/348 (8%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRF---YEFE-GGFEVFKRIGSPISEDDLKE 57
+++AV+ GDGIGPE+ + V+++V ++ YE+ G + ++G P E+ +
Sbjct: 3 FKIAVLAGDGIGPEISVQGVEVMSAVCEKFGHKVNYEYAICGADAIDKVGDPFPEETYRV 62
Query: 58 IRKMDAILFGATTTPF--NVPGYRSL----IVTLRKELDLYANLRIIP------------ 99
+ DA+LF A P N P + ++ +RK+L L+AN+R +
Sbjct: 63 CKNADAVLFSAVGDPKFDNDPTAKVRPEQGLLAMRKKLGLFANIRPVQTFKCLVHKSPLR 122
Query: 100 -DLSNGKEIVIVRENTEGLYARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKERNSFI 158
+L G + + +RE T G+Y + +D+A D + T RI K A +R +
Sbjct: 123 AELVEGADFLCIRELTGGMYFGEKYQDNDKAYDTNMYTRPEIERILKVGFEYAMKRRKHL 182
Query: 159 TFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFG 217
T V KANVL R +R+I E+A + V +D+ +K+++ P V++TEN FG
Sbjct: 183 TVVDKANVLASSRLWRQIAQEMAPQYPEVTTDYMFVDNAAMKMIQEPKFFDVMVTENTFG 242
Query: 218 DIISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDY 277
DI++D ++ +GS+G++PS + G+ +FEPIHGS P G IANP+ ILS AM+ +Y
Sbjct: 243 DILTDEGSVISGSMGLLPSASTGESTPVFEPIHGSWPQAKGLNIANPLAQILSVAMLFEY 302
Query: 278 L------GLNGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
L K V + VR + + G T EV I+ I
Sbjct: 303 FDCKAEGALIRKAVDASLDANVRTPEIQVEGGEKFGTKEVGAWIVDYI 350
>pir||T37546 probable isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) - fission
yeast (Schizosaccharomyces pombe)
>gi|2408008|emb|CAB16208.1| (Z99161) putative isocitrate
dehydrogenase (EC 1.1.1.41) [Schizosaccharomyces pombe]
Length = 356
Score = 164 bits (412), Expect = 1e-39
Identities = 109/339 (32%), Positives = 178/339 (52%), Gaps = 23/339 (6%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFE-GGFEVFKRIGSPISEDDLKEIRK 60
Y V +I GDGIG E ++ + + I F E + G E + + ++ +++
Sbjct: 21 YTVTLIPGDGIGRETSNAVTEIFKTANVPIEFEEIDVTGMEKNNKSSGDALHEAIQSLKR 80
Query: 61 MDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRENT 114
L G TPF G+ S V LRKELD+YA+L +I ++ K + I+RENT
Sbjct: 81 NKVGLKGILFTPFEKGGHTSFNVALRKELDIYASLVLIKNIPGFKTRHDNVDFAIIRENT 140
Query: 115 EGLYARDGIGFSDRAI-----DFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK 168
EG Y+ G +++ +IIT ++RIA+FA + A + +T +HKAN++K
Sbjct: 141 EGEYS----GLEHQSVPGVVESLKIITEYKSKRIAQFAFDFALQNGRKSVTCIHKANIMK 196
Query: 169 -GDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATI 226
D FR ++A + + ++ I+D+ ++ V P V++ N++G I+S++ +
Sbjct: 197 LADGLFRRTFYDVANGYDAITPKDLIVDNASMQAVSRPQQFDVLVMPNLYGSILSNIGSA 256
Query: 227 HAGSIGIVPSGNYGDEIALFEP-IHGSAPDIAGKGIANPIGAILSAAMMLDYLGLN--GK 283
G G++P N+G + ALFEP I G+G ANP AILSA +ML +LGL
Sbjct: 257 LVGGPGVIPGANFGRDYALFEPGCRHVGLSITGRGEANPTAAILSACLMLRHLGLKDYAD 316
Query: 284 VVWEATRRYVRYG-NLTPDMGGTATTSEVTKGIISEIYS 321
++ AT + G LT D+GG+A+T + T I+ + S
Sbjct: 317 LINAATYSVIEEGKTLTKDLGGSASTGDFTHAILERMES 355
>gb|AAD56647.1|AF178973_2 (AF178973) dLEU2 [Cloning vector pCS512]
>gi|5929926|gb|AAD56650.1|AF178973_5 (AF178973) LEU2
[Cloning vector pCS512]
>gi|6318857|gb|AAF07046.1|AF178582_1 (AF178582) LEU2p
[Expression vector pCS316]
>gi|6318865|gb|AAF07053.1|AF178583_3 (AF178583) dLEU2
[Expression vector pSB229]
>gi|6457291|gb|AAF09455.1|AF179625_1 (AF179625) dLEU2
[Shuttle vector pCS513]
>gi|6457295|gb|AAF09459.1|AF179625_5 (AF179625) LEU2
[Shuttle vector pCS513]
>gi|6457299|gb|AAF09462.1|AF179626_3 (AF179626) dLEU2
[Expression vector pGP100]
>gi|6457310|gb|AAF09471.1|AF179628_4 (AF179628) dLEU2
[Shuttle vector pHIGEXhOR]
Length = 368
Score = 164 bits (412), Expect = 1e-39
Identities = 124/358 (34%), Positives = 191/358 (52%), Gaps = 45/358 (12%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTD---RIRFYEFEG---GFEVFKRIGSPISEDDLK 56
++ V+ GD +G E+ AI+V+ +++D ++F +FE G G P+ ++ L+
Sbjct: 10 KIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKF-DFENHLIGGAAIDATGVPLPDEALE 68
Query: 57 EIRKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIP 99
+K+DA+L GA P G ++ +RKEL LYANLR I P
Sbjct: 69 ASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYANLRPCNFASDSLLDLSPIKP 128
Query: 100 DLSNGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKER 154
+ G + V+VRE G+Y DG G + + + + ++ R+A F + L E
Sbjct: 129 QFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYTVPEVQRITRMAAF-MALQHEP 187
Query: 155 NSFITFVHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGVIL 211
I + KANVL R +R+ V E + E ++V+ +IDS + LVKNP +G+I+
Sbjct: 188 PLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNGIII 247
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGD------EIALFEPIHGSAPDIAGKGIANPI 265
T NMFGDIISD A++ GS+G++PS + L+EP HGSAPD+ K +PI
Sbjct: 248 TSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLP-KNKVDPI 306
Query: 266 GAILSAAMMLDYLGLN----GKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
ILSAAMML L LN GK + +A ++ + G T D+GG+ +T+EV + E+
Sbjct: 307 ATILSAAMMLK-LSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAEEV 363
>sp|P34733|LEU3_PICAN 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|629921|pir||S43454 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - yeast (Pichia angusta)
>gi|392893|gb|AAA19109.1| (U00889) beta-isopropylmalate
dehydrogenase [Pichia angusta]
Length = 362
Score = 164 bits (410), Expect = 2e-39
Identities = 123/359 (34%), Positives = 190/359 (52%), Gaps = 50/359 (13%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRI----RFYEFEGGFEVFKRIGSPISEDDLKEIR 59
+ ++ GD +GPEVV A++V+ +V+ I F + G G P+S++ L+ +
Sbjct: 5 IVLLPGDHVGPEVVAEAVKVLEAVSSAIGVKFNFSKHLIGGASIDAYGVPLSDEALEAAK 64
Query: 60 KMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIPDLS 102
K DA+L GA P G ++ +RKEL+LYANLR + ++
Sbjct: 65 KADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELNLYANLRPCSFASDALLKLSPLKSEIV 124
Query: 103 NGKEIVIVRENTEGLY-------ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN 155
G + V+VRE G+Y A DG+ A D ++ +RI + A LA + +
Sbjct: 125 KGTDFVVVRELVGGIYFGDRKEDAGDGV-----ASDTESYSVPEVQRITRMAAFLALQSD 179
Query: 156 SFITF--VHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGVI 210
+ + KANVL R +R+ V E + E + V+ +IDS + LVK+P +GVI
Sbjct: 180 PPLPLWSLDKANVLASSRLWRKTVEETIKNEFPQLTVQHQLIDSAAMILVKSPTKLNGVI 239
Query: 211 LTENMFGDIISDLATIHAGSIGIVPSGNYGD------EIALFEPIHGSAPDIAGKGIANP 264
+T NMFGDIISD A++ GS+G++PS + L+EP HGSAPD+ G G NP
Sbjct: 240 VTNNMFGDIISDEASVIPGSLGLLPSASLASLPDTNKAFGLYEPCHGSAPDL-GPGKVNP 298
Query: 265 IGAILSAAMMLDYLGLN----GKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
+ ILSAAMML L L+ G+ + +A + + G +T D+GG+++T EV + E+
Sbjct: 299 LATILSAAMMLK-LSLDLVDAGRAIEQAVKNVLDAGIMTADLGGSSSTQEVGDAVAQEV 356
>gi|5031777 isocitrate dehydrogenase 3 (NAD+) alpha [Homo sapiens]
>gi|1708399|sp|P50213|IDHA_HUMAN ISOCITRATE
DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|1085346|pir||S55282 isocitrate dehydrogenase (NAD+)
(EC 1.1.1.41) alpha chain precursor - human
>gi|706839|gb|AAA85639.1| (U07681) NAD(H)-specific
isocitrate dehydrogenase alpha subunit precursor [Homo
sapiens]
Length = 366
Score = 164 bits (410), Expect = 2e-39
Identities = 115/339 (33%), Positives = 183/339 (53%), Gaps = 25/339 (7%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSP-----ISEDDLKEI 58
V +I GDGIGPE+ + +++ ++ I++ E I P I + + +
Sbjct: 34 VTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERN-----VTAIQGPGGKWMIPSEAKESM 88
Query: 59 RKMDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRE 112
K L G TP G+ S+ + LRK DLYAN+R + K IV +RE
Sbjct: 89 DKNKMGLKGPLKTPI-AAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRE 147
Query: 113 NTEGLYARDGIGFSDRAID-FRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK-G 169
NTEG Y+ D + ++IT ++RIA+FA A+ + S +T VHKAN+++
Sbjct: 148 NTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMS 207
Query: 170 DRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHA 228
D F + E+AE + ++ E +D+ + +V++P V++ N++GDI+SDL
Sbjct: 208 DGLFLQKCREVAESCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLI 267
Query: 229 GSIGIVPSGNYG-DEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVV 285
G +G+ PSGN G + +A+FE +HG+APDIAGK +ANP +LSA MML ++GL + +
Sbjct: 268 GGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARI 327
Query: 286 WEATRRYVRYG-NLTPDMGGTATTSEVTKGIISEIYSFD 323
A ++ G +LT D+GG A S+ T+ I + D
Sbjct: 328 EAACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKDLD 366
>gi|6324709 NAD+-dependent isocitrate dehydrogenase; Idh2p [Saccharomyces
cerevisiae] >gi|124159|sp|P28241|IDH2_YEAST ISOCITRATE
DEHYDROGENASE [NAD] SUBUNIT 2, MITOCHONDRIAL PRECURSOR
(ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|101418|pir||A39309 isocitrate dehydrogenase (NAD+)
(EC 1.1.1.41) chain IDH2 precursor - yeast
(Saccharomyces cerevisiae) >gi|171747|gb|AAA34702.1|
(M74131) isocitrate dehydrogenase kinase/phosphatase
[Saccharomyces cerevisiae] >gi|1164979|emb|CAA64054.1|
(X94335) YOR3326w [Saccharomyces cerevisiae]
>gi|1420347|emb|CAA99335.1| (Z75043) ORF YOR136w
[Saccharomyces cerevisiae]
Length = 369
Score = 163 bits (409), Expect = 2e-39
Identities = 111/335 (33%), Positives = 183/335 (54%), Gaps = 19/335 (5%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKM 61
Y V+ I+GDGIGPE+ S ++ ++ I + + +F + I + ++ I K
Sbjct: 37 YTVSFIEGDGIGPEISKSVKKIFSAANVPIEWESCDVS-PIFVNGLTTIPDPAVQSITKN 95
Query: 62 DAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRENTE 115
L G TP G+RSL +TLRK L+AN+R + K ++V++RENTE
Sbjct: 96 LVALKGPLATPIG-KGHRSLNLTLRKTFGLFANVRPAKSIEGFKTTYENVDLVLIRENTE 154
Query: 116 GLYAR-DGIGFSDRAIDFRIITLEGARRIAKFAINLAKE-RNSFITFVHKANVLK-GDRF 172
G Y+ + I ++IT + + R+ ++A A+ + VHK+ + + D
Sbjct: 155 GEYSGIEHIVCPGVVQSIKLITRDASERVIRYAFEYARAIGRPRVIVVHKSTIQRLADGL 214
Query: 173 FREIVLEIA-EREGVEVREAIIDSFMIKLVKNP--WDHGVILTENMFGDIISDLAT-IHA 228
F + E++ E + + +ID+ ++K+V NP + V + N++GDI+SDL + + A
Sbjct: 215 FVNVAKELSKEYPDLTLETELIDNSVLKVVTNPSAYTDAVSVCPNLYGDILSDLNSGLSA 274
Query: 229 GSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLNGKV--VW 286
GS+G+ PS N G +I++FE +HGSAPDIAG+ ANP +LS+ MML+++GL +
Sbjct: 275 GSLGLTPSANIGHKISIFEAVHGSAPDIAGQDKANPTALLLSSVMMLNHMGLTNHADQIQ 334
Query: 287 EATRRYVRYG--NLTPDMGGTATTSEVTKGIISEI 319
A + G N T D+ GTATTS T+ +I +
Sbjct: 335 NAVLSTIASGPENRTGDLAGTATTSSFTEAVIKRL 369
>sp|P41563|IDHA_BOVIN ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
(SUBUNITS 3/4) >gi|2117494|pir||S58435 isocitrate
dehydrogenase (NAD+) (EC 1.1.1.41) chain 3/4 precursor -
bovine >gi|902743|gb|AAC18425.1| (U07980) NAD+-dependent
isocitrate dehydrogenase [Bos taurus]
Length = 366
Score = 163 bits (409), Expect = 2e-39
Identities = 114/339 (33%), Positives = 183/339 (53%), Gaps = 25/339 (7%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSP-----ISEDDLKEI 58
V +I GDGIGPE+ + +++ ++ I++ E I P I + + +
Sbjct: 34 VTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERN-----VAAIQGPGGKWMIPPEAKESM 88
Query: 59 RKMDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRE 112
K L G TP G+ S+ + LRK DLYAN+R + K IV +RE
Sbjct: 89 DKNKMGLKGPLKTPI-AAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYHDVNIVTIRE 147
Query: 113 NTEGLYARDGIGFSDRAID-FRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK-G 169
NTEG Y+ D + ++IT ++RIA+FA A+ + S +T VHKAN+++
Sbjct: 148 NTEGEYSGIEHVIVDGVVQSIKLITEAASKRIAEFAFEYARNNHRSNVTAVHKANIMRMS 207
Query: 170 DRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHA 228
D F + E+AE + ++ E +D+ + +V++P V++ N++GDI+SDL
Sbjct: 208 DGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLI 267
Query: 229 GSIGIVPSGNYG-DEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVV 285
G +G+ PSGN G + +A+FE +HG+APDIAGK +ANP +LSA MML ++GL + +
Sbjct: 268 GGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAAKI 327
Query: 286 WEATRRYVRYG-NLTPDMGGTATTSEVTKGIISEIYSFD 323
A ++ G +LT D+GG + S+ T+ I + D
Sbjct: 328 ETACFATIKDGKSLTKDLGGNSKCSDFTEEICRRVKDLD 366
>sp|P73960|LEU3_SYNY3 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|7431318|pir||S75467 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Synechocystis sp. (strain
PCC 6803) >gi|1653112|dbj|BAA18028.1| (D90911)
3-isopropylmalate dehydrogenase [Synechocystis sp.]
Length = 362
Score = 163 bits (409), Expect = 2e-39
Identities = 112/312 (35%), Positives = 168/312 (52%), Gaps = 42/312 (13%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRF-YEFEG---GFEVFKRIGSPISEDDLKE 57
Y V ++ GDGIGPE++ A+ V+ V D+ F + F+ G G P+ E L +
Sbjct: 5 YNVTLLPGDGIGPEIMAVAVAVLGKVADQFGFAFNFQEALIGGAAIDATGQPLPEATLAQ 64
Query: 58 IRKMDAILFGATTTPFNVPGY-----------RSLIVTLRKELDLYANLR---IIPDLSN 103
+ DA+L A + GY + ++ +R+ L L+ANLR I P L +
Sbjct: 65 AKDSDAVLLAA------IGGYAWDNLPRSQRPETGLLAIREGLGLFANLRPATIFPQLID 118
Query: 104 ----------GKEIVIVRENTEGLYARDGIGFSD------RAIDFRIITLEGARRIAKFA 147
G +I++VRE T G+Y G + R ++ T+ RIAK A
Sbjct: 119 ASSLKREVVEGVDIMVVRELTGGIYFGKPKGIFETETGEKRGVNTMAYTVGEIDRIAKVA 178
Query: 148 INLAKERNSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWD 206
A++R + V KANVL + +R+ V+ IA + VE+ +D+ ++LV++P
Sbjct: 179 FETARKRRGQLCSVDKANVLDVSQLWRDRVMAIAVDYPDVELSHLYVDNAAMQLVRSPRQ 238
Query: 207 HGVILTENMFGDIISDLATIHAGSIGIVPSGNYG-DEIALFEPIHGSAPDIAGKGIANPI 265
I+T N+FGDI+SD+A + GSIG++PS + G D LFEP+HGSAPDIAG+ ANP+
Sbjct: 239 FDTIVTGNLFGDILSDIAAMLTGSIGMLPSASLGSDGPGLFEPVHGSAPDIAGQDKANPL 298
Query: 266 GAILSAAMMLDY 277
+LSAAMML Y
Sbjct: 299 AQVLSAAMMLRY 310
>sp|Q56268|LEU3_THIFE 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|478430|pir||JX0286 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Thiobacillus ferrooxidans
>gi|3318965|pdb|1A05|A Chain A, Crystal Structure Of The
Complex Of 3-Isopropylmalate Dehydrogenase From
Thiobacillus Ferrooxidans With 3-Isopropylmalate
>gi|3318966|pdb|1A05|B Chain B, Crystal Structure Of The
Complex Of 3-Isopropylmalate Dehydrogenase From
Thiobacillus Ferrooxidans With 3-Isopropylmalate
>gi|414355|dbj|BAA03437.1| (D14585) 3-isopropylmalate
dehydrogenase [Thiobacillus ferrooxidans]
Length = 358
Score = 163 bits (408), Expect = 3e-39
Identities = 122/347 (35%), Positives = 178/347 (51%), Gaps = 33/347 (9%)
Query: 1 MYRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLK 56
M ++A+ GDGIGPE+V +A +V+++V +R E G P+ L+
Sbjct: 1 MKKIAIFAGDGIGPEIVAAARQVLDAVDQAAHLGLRCTEGLVGGAALDASDDPLPAASLQ 60
Query: 57 EIRKMDAILFGATTTP---FNVPGYRSL--IVTLRKELDLYANLR-------------II 98
DA++ GA P P R ++ LRK LDLYANLR +
Sbjct: 61 LAMAADAVILGAVGGPRWDAYPPAKRPEQGLLRLRKGLDLYANLRPAQIFPQLLDASPLR 120
Query: 99 PDLSNGKEIVIVRENTEGLYARDGIGFS-----DRAIDFRIITLEGARRIAKFAINLAKE 153
P+L +I++VRE T +Y G R + + + RRIA A A+
Sbjct: 121 PELVRDVDILVVRELTGDIYFGQPRGLEVIDGKRRGFNTMVYDEDEIRRIAHVAFRAAQG 180
Query: 154 RNSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILT 212
R + V KANVL+ R +RE+V E+A + V + +D+ ++L++ P V+LT
Sbjct: 181 RRKQLCSVDKANVLETTRLWREVVTEVARDYPDVRLSHMYVDNAAMQLIRAPAQFDVLLT 240
Query: 213 ENMFGDIISDLATIHAGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAA 272
NMFGDI+SD A+ GSIG++PS + G+ A++EPIHGSAPDIAG+ ANP+ ILS A
Sbjct: 241 GNMFGDILSDEASQLTGSIGMLPSASLGEGRAMYEPIHGSAPDIAGQDKANPLATILSVA 300
Query: 273 MMLDYLGLNGKV----VWEATRRYVRYGNLTPDMGGTATTSEVTKGI 315
MML + LN + V A +R + G T D+ T TK +
Sbjct: 301 MMLRH-SLNAEPWAQRVEAAVQRVLDQGLRTADIAAPGTPVIGTKAM 346
>sp|P23390|LEU3_KLULA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|283086|pir||S25369 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - yeast (Kluyveromyces
marxianus var. lactis) >gi|2855|emb|CAA46514.1| (X65545)
3-isopropylmalate dehydrogenase [Kluyveromyces lactis]
>gi|11344907|gb|AAG34541.1| (AF298792)
beta-isopropylmalate dehydrogenase [PCR template vector
pJJH727]
Length = 362
Score = 162 bits (405), Expect = 7e-39
Identities = 121/359 (33%), Positives = 184/359 (50%), Gaps = 49/359 (13%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTD-----RIRFYEFEGGFEVFKRIGSPISEDDLKEI 58
+ V+ GD +G EV D AI+V+N++ + + F G G+P+ ++ L+
Sbjct: 5 IVVLPGDHVGKEVTDEAIKVLNAIAEVRPEIKFNFQHHLIGGAAIDATGTPLPDEALEAS 64
Query: 59 RKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIPDL 101
+K DA+L GA P G ++ +RKEL LYANLR + P+
Sbjct: 65 KKADAVLLGAVGGPKWGTGAVRPEQGLLKIRKELGLYANLRPCNFASDSLLDLSPLKPEY 124
Query: 102 SNGKEIVIVRENTEGLY-------ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKER 154
+ G + V+VRE G+Y DG+ + D ++ +RI + A LA ++
Sbjct: 125 AKGTDFVVVRELVGGIYFGERKEDEGDGVAW-----DSEKYSVPEVQRITRMAAFLALQQ 179
Query: 155 NSFITF--VHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGV 209
N + + KANVL R +R+ V E + E + V+ +IDS + LVK+P +GV
Sbjct: 180 NPPLPIWSLDKANVLASSRLWRKTVEETIKTEFPQLTVQHQLIDSAAMILVKSPTKLNGV 239
Query: 210 ILTENMFGDIISDLATIHAGSIGIVPSGNYGD------EIALFEPIHGSAPDIAGKGIAN 263
++T NMFGDIISD A++ GS+G++PS + L+EP HGSAPD+ + N
Sbjct: 240 VITNNMFGDIISDEASVIPGSLGLLPSASLASLPDTNKAFGLYEPCHGSAPDLPANKV-N 298
Query: 264 PIGAILSAAMMLDY---LGLNGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
PI ILSAAMML L G+ + EA R + G T D+GG+ +T+EV I +
Sbjct: 299 PIATILSAAMMLKLSLDLVEEGRALEEAVRNVLDAGVRTGDLGGSNSTTEVGDAIAKAV 357
>sp|P18120|LEU3_YARLI 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|173250|gb|AAA35244.1| (M37309)
beta-isopropylmalate dehydrogenase (EC 1.1.1.85)
[Yarrowia lipolytica]
>gi|7839536|gb|AAF70314.1|AF260230_1 (AF260230) Leu2
[Yarrowia lipolytica]
Length = 405
Score = 162 bits (405), Expect = 7e-39
Identities = 129/355 (36%), Positives = 188/355 (52%), Gaps = 51/355 (14%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRF-YEFEG---GFEVFKRIGSPISEDDLKEI 58
++ ++ GD GPEV+ A++V+ SV + + FE G ++ G PI++ L
Sbjct: 14 KIVLLGGDFCGPEVIAEAVKVLKSVAEASGTEFVFEDRLIGGAAIEKEGEPITDATLDIC 73
Query: 59 RKMDAILFGAT----TTPFNVPGYRSLI------VTLRKELDLYANLRI-------IPDL 101
RK D+I+ GA T + P R+ + + LRK+L+LYANLR + DL
Sbjct: 74 RKADSIMLGAVGGAANTVWTTPDGRTDVRPEQGLLKLRKDLNLYANLRPCQLLSPKLADL 133
Query: 102 S-----NGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLA 151
S G + +IVRE G+Y DG G A D ++ RIA+ A LA
Sbjct: 134 SPIRNVEGTDFIIVRELVGGIYFGERKEDDGSGV---ASDTETYSVPEVERIARMAAFLA 190
Query: 152 KERNSFITF--VHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD- 206
+ N + + KANVL R +R+ V + + E +E+ +IDS + L+K P
Sbjct: 191 LQHNPPLPVWSLDKANVLASSRLWRKTVTRVLKDEFPQLELNHQLIDSAAMILIKQPSKM 250
Query: 207 HGVILTENMFGDIISDLATIHAGSIGIVPSGNY------GDEIALFEPIHGSAPDIAGKG 260
+G+I+T NMFGDIISD A++ GS+G++PS + + L+EP HGSAPD+ GK
Sbjct: 251 NGIIITTNMFGDIISDEASVIPGSLGLLPSASLASLPDTNEAFGLYEPCHGSAPDL-GKQ 309
Query: 261 IANPIGAILSAAMMLDYLGLN----GKVVWEATRRYVRYGNLTPDMGGTATTSEV 311
NPI ILSAAMML + LN G V A + V G T D+GG+++TSEV
Sbjct: 310 KVNPIATILSAAMMLKF-SLNMKPAGDAVEAAVKESVEAGITTADIGGSSSTSEV 363
>gb|AAA62618.1| (L14756) 3-isopropylmalate dehydrogenase [Cloning vector YIp351]
>gi|1546043|gb|AAB16841.1| (U64693) Leu2 [Cloning vector
pRSQ2-LEU2]
Length = 364
Score = 162 bits (405), Expect = 7e-39
Identities = 124/358 (34%), Positives = 189/358 (52%), Gaps = 45/358 (12%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTD---RIRFYEFEG---GFEVFKRIGSPISEDDLK 56
++ V+ GD +G E+ AI+V+ +++D ++F +FE G G P+ ++ L+
Sbjct: 6 KIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKF-DFENHLIGGAAIDATGVPLPDEALE 64
Query: 57 EIRKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIP 99
+K+DA+L GA P G ++ +RKEL LYANLR I P
Sbjct: 65 ASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYANLRPCNFASDSLLDLSPIKP 124
Query: 100 DLSNGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKER 154
+ G + V+VRE G+Y DG G + + + + ++ R+A F + L E
Sbjct: 125 QFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYTVPEVQRITRMAAF-MALQHEP 183
Query: 155 NSFITFVHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGVIL 211
I + KANVL R +R+ V E + E ++V+ +IDS + LVKNP +G+I+
Sbjct: 184 PLPIWTLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNGIII 243
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGD------EIALFEPIHGSAPDIAGKGIANPI 265
T NMFGDIISD A + GS+G++PS + L+EP HGSAP A K +PI
Sbjct: 244 TSNMFGDIISDEALRYPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPGFA-KNKVDPI 302
Query: 266 GAILSAAMMLDYLGLN----GKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
ILSAAMML L LN GK + +A ++ + G T D+GG+ +T+EV + E+
Sbjct: 303 ATILSAAMMLK-LSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAEEV 359
>gb|AAC53643.1| (U38900) 3-isopropylmalate dehydrogenase [Cloning vector pClasper]
Length = 366
Score = 161 bits (403), Expect = 1e-38
Identities = 124/360 (34%), Positives = 192/360 (52%), Gaps = 47/360 (13%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTD---RIRFYEFEG---GFEVFKRIGSPISEDDL- 55
++ V+ GD +G E+ AI+V+ +++D ++F +FE G G P+ +++L
Sbjct: 6 KIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKF-DFENHLIGGAAIDATGVPLPDEELA 64
Query: 56 -KEIRKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------I 97
+ +K+DA+L GA P G ++ +RKEL LYANLR I
Sbjct: 65 LEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYANLRPCNFASDSLLDLSPI 124
Query: 98 IPDLSNGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAK 152
P + G + V+VRE G+Y DG G + + + + ++ R+A F + L
Sbjct: 125 KPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYTVPEVQRITRMAAF-MALQH 183
Query: 153 ERNSFITFVHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGV 209
E I + KANVL R +R+ V E + E ++V+ +IDS + LVKNP +G+
Sbjct: 184 EPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNGI 243
Query: 210 ILTENMFGDIISDLATIHAGSIGIVPSGNYGD------EIALFEPIHGSAPDIAGKGIAN 263
I+T NMFGDIISD A++ GS+G++PS + L+EP HGSAPD+ K +
Sbjct: 244 IITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLP-KNKVD 302
Query: 264 PIGAILSAAMMLDYLGLN----GKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
PI ILSAAMML L LN GK + +A ++ + G T D+GG+ +T+EV + E+
Sbjct: 303 PIATILSAAMMLK-LSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAEEV 361
>gb|AAG31928.1|AF200469_2 (AF200469) leuB [Buchnera aphidicola]
Length = 363
Score = 161 bits (402), Expect = 2e-38
Identities = 112/315 (35%), Positives = 166/315 (52%), Gaps = 32/315 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
YR+AV+ GDGIGPEV+ A +++N + + + EF G + G+ + + L
Sbjct: 5 YRIAVLPGDGIGPEVMREAYKILNILKNHFSLPLETREFNIGGSAIDQEGTALPKKTLLG 64
Query: 58 IRKMDAILFGAT------TTPFNVPGYRSLIVTLRKELDLYANLR-------------II 98
DAILFG+ P N R+ ++ LRK +L+ANLR +
Sbjct: 65 CENSDAILFGSVGGKKWDDLPINQRPERASLLPLRKHFNLFANLRPAKLYSELKYLSPLR 124
Query: 99 PDL-SNGKEIVIVRENTEGLYARDGIGFSDR-----AIDFRIITLEGARRIAKFAINLAK 152
D+ NG +I+ +RE T GLY G + A D I RIA A LA+
Sbjct: 125 SDIIKNGFDILCIRELTGGLYFGKPTGRLKKNNIEYAFDTEIYYNYEITRIAHLAFQLAQ 184
Query: 153 ERNSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVIL 211
RN I + K+NVL +REIV ++++ V + ID+ ++++K+P ++L
Sbjct: 185 TRNFKICSIDKSNVLNSSVLWREIVKKVSKNYPDVHLSHLYIDNATMQIIKDPNQFDILL 244
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGD-EIALFEPIHGSAPDIAGKGIANPIGAILS 270
N+FGDIISD I GSIG++PS + + + L+EP GSAPDI GK IANPI ILS
Sbjct: 245 CSNLFGDIISDECAIITGSIGMLPSASLNEKKFGLYEPAGGSAPDIEGKNIANPIAQILS 304
Query: 271 AAMMLDYLGLNGKVV 285
+M++ Y G+N K +
Sbjct: 305 VSMLVRY-GMNLKTI 318
>gi|10956836 3-isopropylmalate dehydrogenase [Sphingomonas aromaticivorans]
>gi|11251547|pir||T31128 3-isopropylmalate dehydrogenase
(EC 1.1.1.85) - Sphingomonas aromaticivorans plasmid
pNL1 >gi|3378269|gb|AAD03852.1| (AF079317)
3-isopropylmalate dehydrogenase [Sphingomonas
aromaticivorans]
Length = 355
Score = 160 bits (400), Expect = 3e-38
Identities = 117/350 (33%), Positives = 181/350 (51%), Gaps = 33/350 (9%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
Y +A I GDGIG EVV +AI V+ + + + F F+ + + G + +D L
Sbjct: 4 YSIASIPGDGIGREVVPAAIEVLTAAAAKCGFVLEFRHFDWSCQTYLETGKMMPDDGLDR 63
Query: 58 IRKMDAILFGATTTPFNVPGYRSL---IVTLRKELDLYANLR---IIPDLSNGK------ 105
+R DA+ GA P VP + SL ++ +R+E + Y NLR ++P +++
Sbjct: 64 LRDHDAVFLGAVGFP-GVPDHISLWGLLLPIRREFEQYVNLRPVRLLPGIASPLRDKAPG 122
Query: 106 --EIVIVRENTEGLYA----RDGIGFSDRAIDFRIITLEGARRIAKFAINLAKERNS-FI 158
+ +VREN+EG Y+ R G G ++ + I + G RI ++A + A+ +
Sbjct: 123 DIDFWVVRENSEGEYSQIGGRTGTGENEIVVQQAIFSRRGTDRILRYAFDFARRSGRRHV 182
Query: 159 TFVHKANVLKGDR-FFREIVLEI-AEREGVEVREAIIDSFMIKLVKNPWDHGVILTENMF 216
T K+N L F+ E I AE V + +D + V +P V++ N+F
Sbjct: 183 TSATKSNGLYHSMPFWDERFAAIGAEYPDVAADQYHVDILAARFVMSPERFNVVVGSNLF 242
Query: 217 GDIISDLATIHAGSIGIVPSGNYGDEI---ALFEPIHGSAPDIAGKGIANPIGAILSAAM 273
GDI+SDL G+I + PS N E ++FEP+HGSAPDIAG+GIANPIG I S AM
Sbjct: 243 GDILSDLGPGITGTIAVAPSANLNPERRFPSMFEPVHGSAPDIAGQGIANPIGQIWSGAM 302
Query: 274 MLDYLG--LNGKVVWEATRRYVRYGNL--TPDMGGTATTSEVTKGIISEI 319
ML++LG +V A + ++ TPD+GG TT+ + + S +
Sbjct: 303 MLEHLGEAEPAALVMRALETALIAPDIARTPDLGGNGTTAGFAQAVRSTL 352
>gb|AAC69609.1| (AF045154) NAD-dependent isocitrate dehydrogenase subunit 2
[Kluyveromyces lactis]
Length = 368
Score = 159 bits (399), Expect = 4e-38
Identities = 106/335 (31%), Positives = 187/335 (55%), Gaps = 19/335 (5%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKM 61
Y V+ I+GDG+GPE+ S + ++ I + + +F + I + + I K
Sbjct: 36 YTVSFIEGDGVGPEISKSVKAIFSAAKVPIEWESCDVS-PIFVNGLTTIPDPAVASINKN 94
Query: 62 DAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRENTE 115
L G TP G+RSL +TLRK L+AN+R + K +V++RENTE
Sbjct: 95 LIALKGPLATPIG-KGHRSLNLTLRKTFGLFANVRPAKSIEGYKTTYENVNLVLIRENTE 153
Query: 116 GLYAR-DGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK-GDRF 172
G Y+ + + ++IT + + R+ ++A A+ + S + VHK+ + + D
Sbjct: 154 GEYSGIEHVVAPGVVQSIKLITQDASERVIRYAFEYARAVDRSKVLVVHKSTIQRLADGL 213
Query: 173 FREIVLEIA-EREGVEVREAIIDSFMIKLVKNP--WDHGVILTENMFGDIISDLAT-IHA 228
F ++ +++ E +E++ ++D+ ++K V++P +D V++ N++GDI+SDL + + A
Sbjct: 214 FVDVAKKLSSEYPDIELQTELLDNTVLKTVQHPEAYDDVVVVCPNLYGDILSDLNSGLSA 273
Query: 229 GSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVVW 286
GS+G+ PS N G +++FE +HGSAPDIAG+ ANP +LS+ MML+++GL + +
Sbjct: 274 GSLGLTPSANIGHTVSIFEAVHGSAPDIAGQNKANPTALLLSSVMMLNHMGLTEHADKIE 333
Query: 287 EATRRYVRYG--NLTPDMGGTATTSEVTKGIISEI 319
+A + N T D+GG+A+TS T +I +
Sbjct: 334 KAVLTTIASDAKNRTGDLGGSASTSSFTDAVIERL 368
>pir||E82564 3-isopropylmalate dehydrogenase XF2372 [imported] - Xylella
fastidiosa (strain 9a5c)
>gi|9107550|gb|AAF85171.1|AE004047_2 (AE004047)
3-isopropylmalate dehydrogenase [Xylella fastidiosa]
Length = 357
Score = 159 bits (399), Expect = 4e-38
Identities = 108/305 (35%), Positives = 165/305 (53%), Gaps = 30/305 (9%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKEI 58
++ ++ GDGIG E+V A++V+ V R +R G +++ GSP++++ L
Sbjct: 4 QILILPGDGIGHEIVAEAVKVLEHVNVRHTLNLRLSFDALGGAAYEKYGSPLADETLARA 63
Query: 59 RKMDAILFGATTTP---FNVPGYRSL--IVTLRKELDLYANLR-------------IIPD 100
R DA+L GA P P R ++ +R +L L+ANLR + P+
Sbjct: 64 RDADAVLLGAVGGPQWDTIDPALRPERGLLKIRAQLGLFANLRPALLYPQLADASTLKPE 123
Query: 101 LSNGKEIVIVRENTEGLY------ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKER 154
+ G +I+IVRE T GLY +R A D RRI K +A+ R
Sbjct: 124 VVAGLDILIVRELTGGLYFGQPRGSRISKNGEREAFDTLPYCESEIRRILKVGFEMARLR 183
Query: 155 NSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTE 213
+ V KANVL + +R + E+A + V + +D+ ++LV++P VI+TE
Sbjct: 184 GKKLCSVDKANVLASSQLWRTVAGEMAKDYPDVTLSHMYVDNAAMQLVRHPKQFDVIVTE 243
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNY-GDEIALFEPIHGSAPDIAGKGIANPIGAILSAA 272
NMFGDI+SD A++ GSIG++PS + + ++EP HGSAPDIAG+G+ANP+ ILS A
Sbjct: 244 NMFGDILSDQASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGQGVANPLATILSVA 303
Query: 273 MMLDY 277
M+L Y
Sbjct: 304 MLLRY 308
>emb|CAB99456.1| (AJ292544) beta-isopropylmalate dehydrogenase [Zygosaccharomyces
bailii]
Length = 362
Score = 159 bits (399), Expect = 4e-38
Identities = 122/358 (34%), Positives = 182/358 (50%), Gaps = 47/358 (13%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTD-----RIRFYEFEGGFEVFKRIGSPISEDDLKEI 58
+ V+ GD +G E+ AI+V+ ++++ + F G GSP+ ++ L
Sbjct: 5 IVVLPGDHVGQEIAQEAIKVLEAISEVSPKAKFNFQHHLIGGSAIDATGSPLPDEALAAA 64
Query: 59 RKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIPDL 101
+K DA+L GA P G ++ +RKEL LYANLR + P
Sbjct: 65 KKADAVLLGAVGGPKWGTGAVRPEQGLLKIRKELQLYANLRPCNFASESLLELSPLKPQH 124
Query: 102 SNGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKERNS 156
+ G + ++VRE G+Y DG G A D T +RI + A LA + N
Sbjct: 125 ARGTDFIVVRELVGGIYFGQRKEDDGDGV---AWDSERYTKPEVQRITRMAAFLALQHNP 181
Query: 157 FITF--VHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGVIL 211
+ + KANVL R +R+ V E + E + V+ +IDS + L+KNP +G+I+
Sbjct: 182 PLPIWSLDKANVLASSRLWRKTVEETIKNEFPQLTVKHQLIDSAAMILIKNPTQLNGIII 241
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNY------GDEIALFEPIHGSAPDIAGKGIANPI 265
T NMF DIISD A++ GS+G++PS + + L+EP HGSAPD+ KG NP+
Sbjct: 242 TSNMFEDIISDEASVIPGSLGLLPSASLASLPDTNEAFGLYEPCHGSAPDLP-KGKVNPV 300
Query: 266 GAILSAAMMLDYLGLN----GKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
ILSAAMML L LN G+ + A ++ + G T D+GG+ +TSEV I +
Sbjct: 301 AMILSAAMMLK-LSLNMAKEGEALETAVKQVLDSGVRTGDLGGSNSTSEVGDAIAKAV 357
>gi|11498255 isocitrate dehydrogenase, NADP (icd) [Archaeoglobus fulgidus]
>gi|7431238|pir||G69330 isocitrate dehydrogenase (NADP+)
(EC 1.1.1.42) - Archaeoglobus fulgidus
>gi|2649970|gb|AAB90591.1| (AE001060) isocitrate
dehydrogenase, NADP (icd) [Archaeoglobus fulgidus]
>gi|3043374|emb|CAB09535.1| (Z96105) isocitrate
dehydrogenase [Archaeoglobus fulgidus]
Length = 412
Score = 159 bits (398), Expect = 5e-38
Identities = 119/383 (31%), Positives = 186/383 (48%), Gaps = 66/383 (17%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRIR----FYEFEGGFEVFKRIGSPISEDDLKEIR 59
+ +GDGIG +VV +AIRV+++ D+I +++ G + +K G+ + +D L I+
Sbjct: 30 IPYFEGDGIGKDVVPAAIRVLDAAADKIGKEVVWFQVYAGEDAYKLYGNYLPDDTLNAIK 89
Query: 60 KMDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGKE---------IVIV 110
+ L G TTP GYRSL VT+R+ LDLYAN+R + L VI
Sbjct: 90 EFRVALKGPLTTPVG-GGYRSLNVTIRQVLDLYANVRPVYYLKGVPSPIKHPEKVNFVIF 148
Query: 111 RENTEGLYA------------------RDGIGFS---DRAIDFRIITLEGARRIAKFAIN 149
RENTE +YA ++ G + D I + I+ +R+ + AI
Sbjct: 149 RENTEDVYAGIEWPRGSEEALKLIRFLKNEFGVTIREDSGIGIKPISEFATKRLVRMAIR 208
Query: 150 LAKERN-SFITFVHKANVLK-GDRFFREIVLEIAEREGVE-------------------- 187
A E N +T VHK N++K + FR+ E+A++E E
Sbjct: 209 YAIENNRKSVTLVHKGNIMKYTEGAFRDWGYEVAKQEFGEYCITEDELWDKYGGKQPEGK 268
Query: 188 --VREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGSIGIVPSGNYGDEIAL 245
V++ I D+ +++ ++ VI N+ GD +SD A G +GI P N GD I +
Sbjct: 269 IVVKDRIADNMFQQILTRTDEYDVIALPNLNGDYLSDAAAALIGGLGIAPGSNIGDGIGV 328
Query: 246 FEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLN--GKVVWEATRRYVRYGNLTPD-- 301
FEP+HGSAP AG+ NP IL+ A+M +Y+G +++ +A + G +T D
Sbjct: 329 FEPVHGSAPKYAGQNKVNPTAEILTGALMFEYIGWKDASEMIKKAVEMTISSGIVTYDIH 388
Query: 302 --MGGTAT-TSEVTKGIISEIYS 321
MGGT T E + ++ + S
Sbjct: 389 RHMGGTKVGTREFAEAVVENLQS 411
>pir||A72658 probable isocitrate dehydrogenase APE0689 - Aeropyrum pernix
(strain K1) >gi|5104350|dbj|BAA79665.1| (AP000060) 435aa
long hypothetical isocitrate dehydrogenase [Aeropyrum
pernix]
Length = 435
Score = 159 bits (397), Expect = 6e-38
Identities = 126/396 (31%), Positives = 198/396 (49%), Gaps = 73/396 (18%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTD-------RIRFYEFEGGFEVFKRIGSPISEDDLK 56
VA I+GDG+GPEVV+SA++VV++ RI ++E G ++ G + + L+
Sbjct: 38 VAFIRGDGVGPEVVESALKVVDAAVKKVYGGSRRIVWWELLAGHLAREKCGELLPKATLE 97
Query: 57 EIRKMDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIP--------DLSNGKEIV 108
IR L G TP GYRSL V +R+ LDLYAN+R + ++ ++V
Sbjct: 98 GIRLARVALKGPLETPVGT-GYRSLNVAIRQALDLYANIRPVRYYGQPAPHKYADRVDMV 156
Query: 109 IVRENTEGLYA------------------RDGIGFS---DRAIDFRIITLEGARRIAKFA 147
I RENTE +YA + G S D I + I+ RR+ + A
Sbjct: 157 IFRENTEDVYAGIEWPHDSPEAARIRRFLAEEFGISIREDAGIGVKPISRFATRRLMERA 216
Query: 148 INLA-KERNSFITFVHKANVLKGDRF-------------FREIVL---EIAEREG----- 185
+ A + N+ +T +HK N++K FRE V+ E+ E+ G
Sbjct: 217 LEWALRNGNTVVTIMHKGNIMKYTEGAFMRWAYEVALEKFREHVVTEQEVQEKYGGVRPE 276
Query: 186 --VEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGSIGIVPSGNYGDEI 243
+ V + I D+ + +++ PWD+ VI+ N+ GD ISD A+ G IG+ N GD I
Sbjct: 277 GKILVNDRIADNMLQQIITRPWDYQVIVAPNLNGDYISDAASALVGGIGMAAGMNMGDGI 336
Query: 244 ALFEPIHGSAPDIAGKGIANPIGAILSAAMML-DYLGLN--GKVVWEATRRYVRYGNLTP 300
A+ EP+HG+AP AGK + NP ILSA++++ +++G +V A R+ V+ +T
Sbjct: 337 AVAEPVHGTAPKYAGKDLINPSAEILSASLLIGEFMGWREVKSIVEYAIRKAVQSKKVTQ 396
Query: 301 DMG------GTATTSEVTKGIISEIYSFDPFDIDEI 330
D+ TSE T+ +I+ I D D++E+
Sbjct: 397 DLARHMPGVQPLRTSEYTETLIAYI---DEADLNEV 429
>pir||B81270 3-isopropylmalate dehydrogenase (EC 1.1.1.85) Cj1718c [imported] -
Campylobacter jejuni (strain NCTC 11168)
>gi|6969133|emb|CAB73704.1| (AL139079) 3-isopropylmalate
dehydrogenase [Campylobacter jejuni]
Length = 358
Score = 159 bits (397), Expect = 6e-38
Identities = 111/304 (36%), Positives = 168/304 (54%), Gaps = 30/304 (9%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRF-YEFE----GGFEVFKRIGSPISEDDLK 56
Y+VAV+ GDGIGP V+ A++++ + + F +EF GG + G +S++ LK
Sbjct: 4 YKVAVLAGDGIGPLVMKEALKILTFIAQKYNFSFEFNEAKIGGASI-DAYGVALSDETLK 62
Query: 57 EIRKMDAILFGATTTPF--NVP----GYRSLIVTLRKELDLYANLR---IIPDLSN---- 103
+ DAILFG+ P N+P R+ ++ LRK +L+ANLR I L++
Sbjct: 63 LCEQSDAILFGSVGGPKWDNLPIDQRPERASLLPLRKHFNLFANLRPCKIYESLTHASPL 122
Query: 104 -------GKEIVIVRENTEGLY-ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN 155
G +I+ VRE T G+Y + +G + A D I T + RIA+ A A+ R
Sbjct: 123 KNEIIQKGVDILCVRELTGGIYFGKQDLG-KESAYDTEIYTKKEIERIARIAFESARIRK 181
Query: 156 SFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTEN 214
+ + KANVL +RE+V +A + + + + +D+ +++VKNP V+L N
Sbjct: 182 KKVHLIDKANVLASSILWREVVANVAKDYQDINLEYMYVDNAAMQIVKNPSIFDVMLCSN 241
Query: 215 MFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILSAAM 273
+FGDI+SD GS+G++ S + D+ L+EP GSAPDIA IANPI ILSAA+
Sbjct: 242 LFGDILSDELAAINGSLGLLSSASLNDKGFGLYEPAGGSAPDIAHLNIANPIAQILSAAL 301
Query: 274 MLDY 277
ML Y
Sbjct: 302 MLKY 305
>pir||E81130 3-isopropylmalate dehydrogenase NMB1031 [imported] - Neisseria
meningitidis (group B strain MD58)
>gi|7226270|gb|AAF41430.1| (AE002453) 3-isopropylmalate
dehydrogenase [Neisseria meningitidis MC58]
Length = 356
Score = 158 bits (395), Expect = 1e-37
Identities = 121/332 (36%), Positives = 182/332 (54%), Gaps = 34/332 (10%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDR--IRFYEFEG-GFEVFKRIGSPISEDDLKEIRK 60
+A+++GDGIGPE+V +RV++ + + YE+ G E + G P E RK
Sbjct: 5 IAILRGDGIGPEIVAETVRVLDKLIAQGLDAGYEYAPLGGEAYDEYGHPYPEFTQNLCRK 64
Query: 61 MDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLR---IIPDLSN--------- 103
DA+L GA +P R L ++ +RK+L+L+ANLR + P+L+N
Sbjct: 65 ADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFANLRPAVLYPELANASTLKPEIV 124
Query: 104 -GKEIVIVRENTEGLYARD--GIGFSDRAIDFRIITLEGA----RRIAKFAINLAKERNS 156
G +I+IVRE T +Y + GI + T++ + RRIA A A++R+
Sbjct: 125 AGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNTMKYSESEIRRIAHVAFQSAQKRSK 184
Query: 157 FITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTENM 215
+ V KANVL+ +REI EI E VE+ +D+ ++LV+ P VI T N+
Sbjct: 185 KVCSVGKANVLETTELWREIFEEIGKEYPDVELSHMYVDNAAMQLVRAPKQFDVIATGNI 244
Query: 216 FGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILSAAMM 274
FGDI+SD A++ GSIG++PS + + L+EP HGSAPDIAG+ ANP+ ILS AM+
Sbjct: 245 FGDILSDEASMLTGSIGMLPSASLDENGKGLYEPSHGSAPDIAGQNKANPLATILSLAML 304
Query: 275 LDYLGLNGKV----VWEATRRYVRYGNLTPDM 302
L Y LN + V A ++ ++ G T D+
Sbjct: 305 LRY-SLNDEARAQQVENAVQKVLQQGLRTSDI 335
>emb|CAA72702.1| (Y11966) 3-isopropylmalate dehydrogenase [Buchnera aphidicola]
Length = 364
Score = 157 bits (394), Expect = 1e-37
Identities = 108/312 (34%), Positives = 169/312 (53%), Gaps = 40/312 (12%)
Query: 1 MYRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRF----YEFE-GGFEVFKRIGSPISEDDL 55
+Y++AV+ GDGIGPE++ A ++++ + + F +E++ GG + K G+ ++++ +
Sbjct: 5 LYKIAVLPGDGIGPEIIQEAYKIIDVLQKKFSFNVKMHEYDIGGISIDKH-GTALTQETI 63
Query: 56 KEIRKMDAILFGATT------TPFNVPGYRSLIVTLRKELDLYANLRI---------IPD 100
+ DAILFG+ P ++ ++ LRK +L+AN+R I
Sbjct: 64 EGCENSDAILFGSVGGSKWDHLPEIEKPEKAGLLKLRKHFNLFANIRPCKLSQHLLDISP 123
Query: 101 LSN-----GKEIVIVRENTEGLYARDGIGFSDR--------AIDFRIITLEGARRIAKFA 147
L N G +++ +RE T G+Y G S++ A D I +RIAK A
Sbjct: 124 LKNSIIKKGIDLICIRELTGGIY----FGKSNKITNIDDPYAFDTEIYFRSEIKRIAKIA 179
Query: 148 INLAKERNSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWD 206
+A+ R I + KANVLK +R+ V ++A + V++ ID+ ++L+KNP
Sbjct: 180 FQIAQSRKKKIVSIDKANVLKSSILWRDTVNKMAYKFPNVKLEHMYIDNATMELIKNPSS 239
Query: 207 HGVILTENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPI 265
VIL N+FGDIISD GSIG++PS + L+EP GSAP+IAGK IANPI
Sbjct: 240 FDVILCSNLFGDIISDECAAIIGSIGLLPSASLNKSGFGLYEPAGGSAPNIAGKTIANPI 299
Query: 266 GAILSAAMMLDY 277
ILS +M+L Y
Sbjct: 300 AQILSLSMLLKY 311
>dbj|BAB08299.1| (AB007650) 3-isopropylmalate dehydrogenase [Arabidopsis thaliana]
Length = 409
Score = 157 bits (394), Expect = 1e-37
Identities = 108/312 (34%), Positives = 162/312 (51%), Gaps = 42/312 (13%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTD----RIRFYEFEGGFEVFKRIGSPISEDDLKE 57
Y +A++ GDGIGPEV+ A V+ F E G +G P+ E+
Sbjct: 47 YNIALLPGDGIGPEVISVAKNVLQKAGSLEGLEFDFKEMPVGGAALDLVGVPLPEETFTA 106
Query: 58 IRKMDAILFGATTTPFNVPGYR-----------SLIVTLRKELDLYANLR---IIPDL-- 101
+ DAIL GA + GY+ + LR++L ++ANLR ++P L
Sbjct: 107 AKLSDAILLGA------IGGYKWDKNEKHLRPEMALFYLRRDLKVFANLRPATVLPQLVD 160
Query: 102 --------SNGKEIVIVRENTEGLYARDGIGFS------DRAIDFRIITLEGARRIAKFA 147
+ G +++IVRE T G+Y + G + + + I RIA+ A
Sbjct: 161 ASTLKKEVAEGVDMMIVRELTGGIYFGEPRGITINENGEEVGVSTEIYAAHEIDRIARVA 220
Query: 148 INLAKERNSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWD 206
A++R + V KANVL +R+ V +A E VE+ +D+ ++L+++P
Sbjct: 221 FETARKRRGKLCSVDKANVLDASILWRKRVTALASEYPDVELSHMYVDNAAMQLIRDPKQ 280
Query: 207 HGVILTENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPI 265
I+T N+FGDI+SD A++ GSIG++PS + G+ LFEPIHGSAPDIAG+ ANP+
Sbjct: 281 FDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPL 340
Query: 266 GAILSAAMMLDY 277
ILSAAM+L Y
Sbjct: 341 ATILSAAMLLKY 352
>gb|AAG31402.1| (AF197456) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 365
Score = 157 bits (394), Expect = 1e-37
Identities = 109/316 (34%), Positives = 165/316 (51%), Gaps = 33/316 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVV----NSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKE 57
YR+AV+ GDGIGPEV+ A +++ N + I EF G + G + +D L
Sbjct: 5 YRIAVLPGDGIGPEVMKEAYKILKILQNYFSLHIEIKEFNIGGIALDKEGVALPKDTLLG 64
Query: 58 IRKMDAILFGAT------TTPFNVPGYRSLIVTLRKELDLYANLR----------IIP-- 99
DAILFG+ P R+ ++ LRK +L+ NLR + P
Sbjct: 65 CENSDAILFGSVGGKKWNNLPIEKRPERASLLPLRKHFNLFYNLRPAKLYSELRFLSPLR 124
Query: 100 ---DLSNGKEIVIVRENTEGLYARDGIGFSDR-----AIDFRIITLEGARRIAKFAINLA 151
+ NG +I+ +RE T G+Y + G + A D + RIA A LA
Sbjct: 125 SKTXIKNGFDILCIRELTGGIYFGEPRGRLKKNNIEYAFDTEVYYDYEINRIAHVAFQLA 184
Query: 152 KERNSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVI 210
+ R + + + KANVL +REIV ++++ V + ID+ +++++NP ++
Sbjct: 185 RTRKNKVCSIDKANVLNSSLLWREIVTKVSKNYPDVHLSHLYIDNATMQIIQNPNQFDIL 244
Query: 211 LTENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAIL 269
L N+FGDI+SD I GSIG++PS + ++ L+EP GSAPDIAGK IANPI IL
Sbjct: 245 LCSNLFGDILSDECAIITGSIGMLPSASLNEKNFGLYEPAGGSAPDIAGKNIANPIAQIL 304
Query: 270 SAAMMLDYLGLNGKVV 285
S +M++ Y G+N K +
Sbjct: 305 SLSMLVRY-GMNLKKI 319
>sp|P24404|LEU3_AGRTU 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|142237|gb|AAA22089.1| (M38670)
beta-isopropylmalate dehydrogenase [Agrobacterium
tumefaciens]
Length = 370
Score = 157 bits (393), Expect = 2e-37
Identities = 115/332 (34%), Positives = 176/332 (52%), Gaps = 40/332 (12%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTD--RIRFYEFEG--GFEVFKRIGSPISEDDLKEIR 59
V ++ GDGIGPE + +++ + F EG G + G IS+ D+++
Sbjct: 6 VFLLPGDGIGPEAMTEVRKLIEYMNSAHNAGFTVSEGLVGGSAYDAHGVAISDADMEKAL 65
Query: 60 KMDAILFGATTTPF--NVPGYR---SLIVTLRKELDLYANLR-------------IIPDL 101
DAILFGA P VP + ++ LRK+L+L+ANLR + P+L
Sbjct: 66 AADAILFGAVGGPKWDGVPYEHRPEAGLLRLRKDLELFANLRPAICYPALAAASSLKPEL 125
Query: 102 SNGKEIVIVRENTEGLYARDGIGFSD------RAIDFRIITLEGARRIAKFAINLAKERN 155
G +I+IVRE T G+Y + D R ID +I RIA A LA+ R+
Sbjct: 126 VEGLDILIVRELTGGVYFGEPKQIIDLGNGQKRGIDTQIYDTFEIERIASVAFELARSRD 185
Query: 156 SFITFVHKANVLKGDRFFREIVLEI--AEREGVEVREAIIDSFMIKLVKNPWDHGVILTE 213
+ + + K NV+K + ++V E A+ + V++ + D+ ++LV+ P VI+T+
Sbjct: 186 NRVCSMEKRNVMKSGVLWNQVVTETHAAKYKDVQLEHMLADAGGMQLVRKPKQFDVIVTD 245
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNYG-------DEIALFEPIHGSAPDIAGKGIANPIG 266
N+FGD++SD+A + GS+G++PS + G A++EP+HGSAPDIAGK IANPI
Sbjct: 246 NLFGDMLSDVAAMLTGSLGMLPSASLGAPDAKTGKRKAMYEPVHGSAPDIAGKSIANPIA 305
Query: 267 AILSAAMMLDYLGLNGKVVWEATRRYVRYGNL 298
I S AM L Y + +V EAT+ N+
Sbjct: 306 MIASFAMCLRY---SFNMVDEATKLEAAIANV 334
>sp|O14429|LEU3_CANGA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|2231676|gb|AAB62089.1| (U90626) LEU2
[Candida glabrata]
Length = 365
Score = 157 bits (393), Expect = 2e-37
Identities = 118/361 (32%), Positives = 187/361 (51%), Gaps = 52/361 (14%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVT----DRIRF---YEFEGGFEVFKRIGSPISEDDLK 56
+ V GD +G E+ + A +V+N++ D++ F + GG + G P+ ++ L+
Sbjct: 7 IVVPPGDHVGQEITEEANKVLNAIQECRPDKVNFKFDHHLIGGAPI-DATGVPLPDEALE 65
Query: 57 EIRKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIP 99
+K DA+L GA P G ++ +RKEL LYANLR + P
Sbjct: 66 ASKKADAVLLGAVGGPKWGTGAVRPEQGLLKIRKELQLYANLRPCNFASDSLLDLSPLKP 125
Query: 100 DLSNGKEIVIVRENTEGLY-------ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAK 152
+++ G + V+VRE G+Y DG+ + D ++ +RI + A +A
Sbjct: 126 EIARGTDFVVVRELVGGIYFGERKEDEGDGVAW-----DSEKYSVPEVQRITRMAAFMAL 180
Query: 153 ERNSFITF--VHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-H 207
+ N + + KANVL R +R+ V E + E + V+ +IDS + LVKNP +
Sbjct: 181 QHNPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPQLTVQHQLIDSAAMILVKNPTHLN 240
Query: 208 GVILTENMFGDIISDLATIHAGSIGIVPSGNYGD------EIALFEPIHGSAPDIAGKGI 261
G+I+T NMFGDIISD A++ GS+G++PS + L+EP HGSAPD+ KG
Sbjct: 241 GIIITNNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLP-KGK 299
Query: 262 ANPIGAILSAAMMLDY---LGLNGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISE 318
NP+ ILSAAMML L G+++ +A ++ + G T D+ GT +T+EV +
Sbjct: 300 VNPVATILSAAMMLKLSLDLFEEGEIIEQAVKKVLDSGIRTADLKGTNSTTEVGDAVAKA 359
Query: 319 I 319
+
Sbjct: 360 V 360
>gi|10957108 3-isopropylmalate dehydrogenase [Buchnera sp. APS]
>gi|7707822|dbj|BAA95424.1| (AP001071) 3-isopropylmalate
dehydrogenase [Buchnera sp. APS]
Length = 363
Score = 157 bits (393), Expect = 2e-37
Identities = 106/311 (34%), Positives = 165/311 (52%), Gaps = 32/311 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTD----RIRFYEFEGGFEVFKRIGSPISEDDLKE 57
YR+AV+ GDGIGPEV+ A +++N + + +F G +R G + + L
Sbjct: 5 YRIAVLSGDGIGPEVMQEACKILNVLKKYFFLSLEIQKFNIGGIAIEREGVALPKTTLLG 64
Query: 58 IRKMDAILFGAT------TTPFNVPGYRSLIVTLRKELDLYANLR---IIPDLS------ 102
D+IL G+ P R+ ++ LRK +L++NLR + P+L
Sbjct: 65 CENSDSILLGSVGGKKWDNLPVEQRPERAALLPLRKHFNLFSNLRPAKLYPELKCLSPLR 124
Query: 103 -----NGKEIVIVRENTEGLYARDGIGFSDR-----AIDFRIITLEGARRIAKFAINLAK 152
NG +I+ VRE T G+Y + GF ++ A D I RIA A LA+
Sbjct: 125 SDIVKNGFDILCVRELTGGIYFGEPKGFVNKNNTKYAFDTEIYHEYEIIRIAHLAFKLAR 184
Query: 153 ERNSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVIL 211
R + + K+NVL+ +RE+V ++++ V + ID+ ++++K+P V+L
Sbjct: 185 SRKKKVCSIDKSNVLQSSILWREVVESVSKKYPDVHLSHLYIDNAAMQIIKDPNQFDVLL 244
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILS 270
N+FGDIISD GSIG++PS ++ ++ L+EP GSAPDI GK IANPI ILS
Sbjct: 245 CSNLFGDIISDECATITGSIGMLPSASFNEKNFGLYEPAGGSAPDIEGKNIANPIAQILS 304
Query: 271 AAMMLDYLGLN 281
+M++ Y G+N
Sbjct: 305 LSMLVRY-GMN 314
>gb|AAG31378.1| (AF197448) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 364
Score = 157 bits (393), Expect = 2e-37
Identities = 107/308 (34%), Positives = 159/308 (50%), Gaps = 32/308 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
YR+AV+ GDGIGPEV+ A +++N + + EF+ G + G + + L
Sbjct: 5 YRIAVLPGDGIGPEVMREAYKILNILKYHFSLPLEIEEFDVGGSAIDKEGVALPKKTLLG 64
Query: 58 IRKMDAILFGAT------TTPFNVPGYRSLIVTLRKELDLYANLR--------------- 96
DAILFG+ + P + R+ ++ LRK +L+ANLR
Sbjct: 65 CENADAILFGSVGGRKWDSLPIDQRPERASLLPLRKHFNLFANLRPAQLYSELKYLSPLA 124
Query: 97 IIPDLSNGKEIVIVRENTEGLYARDGIGFS-----DRAIDFRIITLEGARRIAKFAINLA 151
+ + NG I+ VRE T G+Y G S + A D + RIA A LA
Sbjct: 125 FLIVIKNGFNILCVRELTGGIYFGQPSGRSKTNNFEYAFDTEVYYDYEINRIAHLAFQLA 184
Query: 152 KERNSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVI 210
+ RN + + K+NVL +REIV +++ V + ID+ ++++K+P V+
Sbjct: 185 QTRNYKVCSIDKSNVLNSSILWREIVKKVSLSYPNVHLSHLYIDNATMQIIKDPNQFDVL 244
Query: 211 LTENMFGDIISDLATIHAGSIGIVPSGNYGD-EIALFEPIHGSAPDIAGKGIANPIGAIL 269
L N+FGDIISD I GSIG++PS + + + L+EP GSAPDI GK IANPI IL
Sbjct: 245 LCSNLFGDIISDECAIITGSIGMLPSASLNEKKFGLYEPAGGSAPDIEGKNIANPIAQIL 304
Query: 270 SAAMMLDY 277
S +M++ Y
Sbjct: 305 SVSMLVRY 312
>gb|AAG31405.1| (AF197457) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 157 bits (393), Expect = 2e-37
Identities = 106/311 (34%), Positives = 165/311 (52%), Gaps = 32/311 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTD----RIRFYEFEGGFEVFKRIGSPISEDDLKE 57
YR+AV+ GDGIGPEV+ A +++N + + +F G +R G + + L
Sbjct: 5 YRIAVLSGDGIGPEVMQEACKILNVLKKYFFLSLEIQKFNIGGIAIEREGVALPKTTLLG 64
Query: 58 IRKMDAILFGAT------TTPFNVPGYRSLIVTLRKELDLYANLR---IIPDLS------ 102
D+IL G+ P R+ ++ LRK +L++NLR + P+L
Sbjct: 65 CENSDSILLGSVGGKKWDNLPVEQRPERAALLPLRKHFNLFSNLRPAKLYPELKCLSPLR 124
Query: 103 -----NGKEIVIVRENTEGLYARDGIGFSDR-----AIDFRIITLEGARRIAKFAINLAK 152
NG +I+ VRE T G+Y + GF ++ A D I RIA A LA+
Sbjct: 125 SDIVKNGFDILCVRELTGGIYFGEPKGFVNKNNTKYAFDTEIYHEYEIIRIAHLAFKLAR 184
Query: 153 ERNSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVIL 211
R + + K+NVL+ +RE+V ++++ V + ID+ ++++K+P V+L
Sbjct: 185 SRKKKVCSIDKSNVLQSSILWREVVESVSKKYPDVHLSHLYIDNAAMQIIKDPNQFDVLL 244
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILS 270
N+FGDIISD GSIG++PS ++ ++ L+EP GSAPDI GK IANPI ILS
Sbjct: 245 CSNLFGDIISDECATITGSIGMLPSASFNEKNFGLYEPAGGSAPDIEGKNIANPIAQILS 304
Query: 271 AAMMLDYLGLN 281
+M++ Y G+N
Sbjct: 305 LSMLVRY-GMN 314
>gi|6324291 alpha-4-beta-4 subunit of mitochondrial isocitrate dehydrogenase 1;
Idh1p [Saccharomyces cerevisiae]
>gi|400041|sp|P28834|IDH1_YEAST ISOCITRATE DEHYDROGENASE
[NAD] SUBUNIT 1, MITOCHONDRIAL PRECURSOR (ISOCITRIC
DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|320779|pir||S31264 isocitrate dehydrogenase (NAD+)
(EC 1.1.1.41) chain IDH1 precursor - yeast
(Saccharomyces cerevisiae) >gi|171766|gb|AAA34711.1|
(M95203) isocitrate dehydrogenase-1 nuclear encoded
[Saccharomyces cerevisiae] >gi|1301886|emb|CAA95904.1|
(Z71313) ORF YNL037c [Saccharomyces cerevisiae]
Length = 360
Score = 157 bits (392), Expect = 2e-37
Identities = 107/344 (31%), Positives = 185/344 (53%), Gaps = 41/344 (11%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVT-----DRIRFYEFEGGFEVFKRIGSPISEDDLK 56
+ V +I GDG+G E+ DS + + + I + + V++ + S
Sbjct: 29 FTVTLIPGDGVGKEITDSVRTIFEAENIPIDWETINIKQTDHKEGVYEAVES-------- 80
Query: 57 EIRKMDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIV 110
+++ L G TP + G+ SL V LRK+LD+YAN+ + L K +++++
Sbjct: 81 -LKRNKIGLKGLWHTPADQTGHGSLNVALRKQLDIYANVALFKSLKGVKTRIPDIDLIVI 139
Query: 111 RENTEGLYARDGIGFSDRAI-----DFRIITLEGARRIAKFAINLAKERN-SFITFVHKA 164
RENTEG ++ G ++ +++T RIA+FA + AK+ N +T VHKA
Sbjct: 140 RENTEGEFS----GLEHESVPGVVESLKVMTRPKTERIARFAFDFAKKYNRKSVTAVHKA 195
Query: 165 NVLK-GDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIIS 221
N++K GD FR I+ EI ++E ++V I+D+ ++ V P V++T +M+G I+
Sbjct: 196 NIMKLGDGLFRNIITEIGQKEYPDIDVSSIIVDNASMQAVAKPHQFDVLVTPSMYGTILG 255
Query: 222 DLATIHAGSIGIVPSGNYGDEIALFEPIHGS---APDIAGKGIANPIGAILSAAMMLDYL 278
++ G G+V N+G + A+FEP GS DI G+ +ANP ILS+ +ML++L
Sbjct: 256 NIGAALIGGPGLVAGANFGRDYAVFEP--GSRHVGLDIKGQNVANPTAMILSSTLMLNHL 313
Query: 279 GLN--GKVVWEATRRYVRYG-NLTPDMGGTATTSEVTKGIISEI 319
GLN + +A + G + T D+GG+++T++ T II+++
Sbjct: 314 GLNEYATRISKAVHETIAEGKHTTRDIGGSSSTTDFTNEIINKL 357
>pir||E81836 probable 3-isopropylmalate dehydrogenase (EC 1.1.1.85) NMA1456
[imported] - Neisseria meningitidis (group A strain
Z2491) >gi|7380107|emb|CAB84691.1| (AL162756) putative
3-isopropylmalate dehydrogenase [Neisseria meningitidis
Z2491]
Length = 356
Score = 157 bits (392), Expect = 2e-37
Identities = 119/332 (35%), Positives = 181/332 (53%), Gaps = 34/332 (10%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEG---GFEVFKRIGSPISEDDLKEIRK 60
+A+++GDGIGPE+V +RV++ + + +E G E + G P E RK
Sbjct: 5 IAILRGDGIGPEIVAETVRVLDKLIAQGLDVGYEYAPLGGEAYDEYGHPYPEFTQNLCRK 64
Query: 61 MDAILFGATTTPFNVPGYRSL-----IVTLRKELDLYANLR---IIPDLSN--------- 103
DA+L GA +P R L ++ +RK+L+L+ANLR + P+L+N
Sbjct: 65 ADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFANLRPAILYPELANASTLKSEIV 124
Query: 104 -GKEIVIVRENTEGLYARD--GIGFSDRAIDFRIITLEGA----RRIAKFAINLAKERNS 156
G +I+IVRE T +Y + GI + T++ + RRIA A A++R+
Sbjct: 125 AGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNTMKYSESEIRRIAHVAFQSAQKRSK 184
Query: 157 FITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTENM 215
+ V KANVL+ +REI EI E VE+ +D+ ++LV+ P VI T N+
Sbjct: 185 KVCSVGKANVLETTELWREIFEEIGKEYPDVELSHMYVDNAAMQLVRAPKQFDVIATGNI 244
Query: 216 FGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILSAAMM 274
FGDI+SD A++ GSIG++PS + + L+EP HGSAPDIAG+ ANP+ +LS AM+
Sbjct: 245 FGDILSDEASMLTGSIGMLPSASLDENGKGLYEPSHGSAPDIAGQNKANPLATVLSLAML 304
Query: 275 LDYLGLNGKV----VWEATRRYVRYGNLTPDM 302
L Y LN + V A ++ ++ G T D+
Sbjct: 305 LRY-SLNDEARAQQVENAVQKVLQQGLRTGDI 335
>gi|10954451 isopropylmalate dehydrogenase [Buchnera aphidicola]
>gi|3282733|gb|AAD12594.1| (AF041836) isopropylmalate
dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 156 bits (391), Expect = 3e-37
Identities = 108/307 (35%), Positives = 162/307 (52%), Gaps = 31/307 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
+R+AV+ GDGIGPEV+ A +++N + + + EF+ G R G + E L+
Sbjct: 5 FRIAVLPGDGIGPEVMREAYKILNILERKFFLKLELREFDIGGIAIDREGIALPEKTLRG 64
Query: 58 IRKMDAILFGATT------TPFNVPGYRSLIVTLRKELDLYANLR---IIPDLSN----- 103
DAILFG+ P R+ ++ LRK +L++NLR + DL N
Sbjct: 65 CENSDAILFGSVGGKKWDYLPIESRPERASLLPLRKHFNLFSNLRPAKLRVDLKNLSPLR 124
Query: 104 ------GKEIVIVRENTEGLYARDGIG-FSDR----AIDFRIITLEGARRIAKFAINLAK 152
G +I+ +RE T G+Y G F ++ A D I RIA A LA
Sbjct: 125 SDIVRDGFDILCIRELTGGIYFGKPKGRFKNKNGEYAFDTEIYYDFEIERIANLAFELAS 184
Query: 153 ERNSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVIL 211
R + + KANVL+ +REIV ++++ V + ID+ ++++KNP ++L
Sbjct: 185 SRRCKVCSIDKANVLESSVLWREIVKKVSKNYPDVHLSHLYIDNACMQIIKNPTQFDILL 244
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILS 270
N+FGDIISD + GSIG++PS + ++ L+EP GSAPDI GK IANPI ILS
Sbjct: 245 CSNIFGDIISDECAMITGSIGMLPSASLNNQKFGLYEPAGGSAPDIEGKNIANPIAQILS 304
Query: 271 AAMMLDY 277
+M++ Y
Sbjct: 305 LSMLIRY 311
>gb|AAG31384.1| (AF197450) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 364
Score = 156 bits (391), Expect = 3e-37
Identities = 112/314 (35%), Positives = 165/314 (51%), Gaps = 33/314 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVV----NSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKE 57
YR+AV+ GDGIGPEV+ A +++ N+ EF G + G + + L
Sbjct: 5 YRIAVLPGDGIGPEVMREAYKILKILKNNFLLSFEIEEFNVGGIAIDQEGLALPKKTLLG 64
Query: 58 IRKMDAILFGAT------TTPFNVPGYRSLIVTLRKELDLYANLR----------IIPDL 101
K DAILFG+ P R+ ++ LRK +L+ANLR + P
Sbjct: 65 CEKSDAILFGSVGGKKWDNFPIEERPERAALLPLRKHFNLFANLRPAKIYSELKHLSPLR 124
Query: 102 SN----GKEIVIVRENTEGLYARDGIGFS------DRAIDFRIITLEGARRIAKFAINLA 151
SN G +I+ +RE T G+Y G + A D I RIA A LA
Sbjct: 125 SNIVRDGFDILCIRELTGGIYFGQPSGRRLEKNNIEYAFDTEIYYDYEINRIAHLAFQLA 184
Query: 152 KERNSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVI 210
+ R+ + + K+NVL ++EIV ++++ V++ ID+ +++++KNP V+
Sbjct: 185 QSRSHKVCSIDKSNVLNSSILWKEIVQKVSKNYPDVDLSHLYIDNAIMQIIKNPNQFDVL 244
Query: 211 LTENMFGDIISDLATIHAGSIGIVPSGNYGD-EIALFEPIHGSAPDIAGKGIANPIGAIL 269
L N+FGDIISD I GSIG++PS + + + L+EP GSAPDIAGK IANPI IL
Sbjct: 245 LCPNLFGDIISDECAIITGSIGMLPSASLNEKKFGLYEPAGGSAPDIAGKNIANPIAQIL 304
Query: 270 SAAMMLDYLGLNGK 283
S +M++ Y G+N K
Sbjct: 305 SLSMLVRY-GMNLK 317
>sp|O60027|LEU3_ASHGO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|3171729|emb|CAA07006.1| (AJ006406)
beta-isopropylmalatedehydrogenase [Eremothecium
gossypii] >gi|3873558|emb|CAA04541.1| (AJ001115)
b-isopropylmalate dehydrogenase [Eremothecium gossypii]
Length = 372
Score = 156 bits (390), Expect = 4e-37
Identities = 124/357 (34%), Positives = 189/357 (52%), Gaps = 51/357 (14%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEV-----FKRIGSPISEDDLKE 57
R+ V+ GD IG EVV+ A++V+ +V + F+ + + GS + ++ L
Sbjct: 6 RIVVLPGDHIGREVVEEAVKVLGAVEQSLSDVHFDFQYHLVGGAAIDATGSALPDEALGA 65
Query: 58 IRKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIPD 100
++ DA+L GA P G ++ LR+EL +YANLR + P+
Sbjct: 66 AKEADAVLLGAVGGPKWQGGAVRPEQGLLKLRQELGVYANLRPCNFAADSLLELSPLRPE 125
Query: 101 LSNGKEIVIVRENTEGLY-------ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKE 153
++ +I++VRE G Y DG+ + D T++ +RIA+ A LA +
Sbjct: 126 IARDTDIMVVRELLGGSYFGERHEDEGDGVAW-----DTDKYTVKEVQRIARMAGFLALQ 180
Query: 154 RNSFITF--VHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HG 208
+ + + KANVL R +R+ V E + E V+++ +IDS + LVKNP +G
Sbjct: 181 HDPPLPVWSLDKANVLASSRLWRKTVEETFQSEFPNVQLQHQLIDSAAMILVKNPRAFNG 240
Query: 209 VILTENMFGDIISDLATIHAGSIGIVPSGNYG------DEIALFEPIHGSAPDIAGKGIA 262
V++T NMFGDIISD A++ GS+G++PS + L+EP HGSAPD+ G A
Sbjct: 241 VVVTSNMFGDIISDEASVIPGSLGLLPSASLASLPDSKSAFGLYEPCHGSAPDLPA-GKA 299
Query: 263 NPIGAILSAAMMLDYLGLN----GKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGI 315
NPIG ILSAAMML L LN G+ V +A + + G T D+ G+++TSEV I
Sbjct: 300 NPIGCILSAAMMLK-LSLNMVAAGEAVEQAVQEVLDSGVRTGDLLGSSSTSEVGDAI 355
>sp|O59930|LEU3_PHACH 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|2935579|gb|AAC26195.1| (AF050668)
3-isopropylmalate dehydrogenase [Phanerochaete
chrysosporium]
Length = 380
Score = 155 bits (389), Expect = 5e-37
Identities = 119/375 (31%), Positives = 190/375 (49%), Gaps = 67/375 (17%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTD-------RIRFYEFEGGFEVFKRIGSPISEDD 54
+++ ++ GDGIGPEVV A RV+ V+ ++ ++F GG + K G P++
Sbjct: 6 FKIVILPGDGIGPEVVAEATRVLEVVSASSSDVEIKLETHDF-GGCSIDKH-GEPLTAAT 63
Query: 55 LKEIRKMDAILFGATTTP-FNVPGY---RSLIVTLRKELDLYANLR-------------- 96
L+ + DAIL GA P + V ++ LRK L LYAN+R
Sbjct: 64 LEACKLADAILLGAIGGPKWGVNSKVRPEQALLALRKALGLYANIRPANFASDSLLAYSP 123
Query: 97 IIPDLSNGKEIVIVRENTEGLY--ARDGIGF---SDRAIDFRIITLEGARRIAKFAINLA 151
+ P ++ G +I+++RE G Y R +G D A D I ++ +RI + +A
Sbjct: 124 LKPSVARGVDIIVIRELIGGAYFGERKELGARAQEDAAWDTMIYSVPEVQRITRSRRQVA 183
Query: 152 KERNSF-ITFVHKANVLKGDRFFREIVLEIAEREGVEVR--EAIIDSFMIKLVKNPWD-H 207
+ + KANVL R +R++ E + E +++ ++DS + +V NP +
Sbjct: 184 SPDPPLPVHSIDKANVLASSRLWRKVATETIQNEFPQLKLDHHLVDSASMLIVANPKKLN 243
Query: 208 GVILTENMFGDIISDLATIHAGSIGIVPSGNYG-------------DEIALFEPIHGSAP 254
GVILTEN+FGDI+SD +++ GS+G++PS + ++EPIHGSAP
Sbjct: 244 GVILTENLFGDILSDESSVIPGSLGLLPSASLAARPLWPNTAHRRSSPRRVYEPIHGSAP 303
Query: 255 DIAGKGIANPIGAILSAAMMLDYLGLNGKVVWEATRRYV-------------RYGNLTPD 301
DIAG+GIANPIG ILSAAM+ + VW + + + R G T D
Sbjct: 304 DIAGQGIANPIGTILSAAML-----CGTRSVWRSPAKAIEAAVEGARRQGDRREGLRTAD 358
Query: 302 MGGTATTSEVTKGII 316
+GG++ T ++ ++
Sbjct: 359 LGGSSKTKDIGHKVV 373
>gb|AAD21684.1| (AC004793) Strong similarity to gb|X59970 3-isopropylmalate
dehydrogenase (IMDH) from Brassica napus. EST gb|F14478
comes from this gene. [Arabidopsis thaliana]
Length = 406
Score = 155 bits (389), Expect = 5e-37
Identities = 107/312 (34%), Positives = 164/312 (52%), Gaps = 42/312 (13%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTD----RIRFYEFEGGFEVFKRIGSPISEDDLKE 57
Y + ++ GDGIGPEV+ A V+ F E G +G P+ E+
Sbjct: 44 YNITLLPGDGIGPEVISVAKNVLQKAGFLQGLEFDFQEMPFGGAALDLVGVPLPEETSTA 103
Query: 58 IRKMDAILFGATTTPFNVPGYR-----------SLIVTLRKELDLYANLR---IIPDL-- 101
++ DAIL GA + GY+ ++ +R++L+++ANLR ++P L
Sbjct: 104 AKQSDAILLGA------IGGYKWDKNEKHLRPEMGLLNIRRDLNVFANLRPATVLPQLVD 157
Query: 102 --------SNGKEIVIVRENTEGLYARDGIGFS------DRAIDFRIITLEGARRIAKFA 147
+ G +++IVRE T G+Y + G + + + I RIA+ A
Sbjct: 158 ASTLKKEVAQGVDMMIVRELTGGIYFGEPRGITINENGEEVGFNTEIYAAHEIDRIARVA 217
Query: 148 INLAKERNSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWD 206
A++R + V KANVL +R+ V +A E VE+ +D+ ++LV++P
Sbjct: 218 FETARKRRGKLCSVDKANVLDASILWRKRVTALASEYPDVELSHMYVDNAAMQLVRDPKQ 277
Query: 207 HGVILTENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPI 265
I+T N+FGDI+SD A++ GSIG++PS + G+ LFEPIHGSAPDIAG+ ANP+
Sbjct: 278 FDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPL 337
Query: 266 GAILSAAMMLDY 277
ILSAAM+L Y
Sbjct: 338 ATILSAAMLLKY 349
>gb|AAG31387.1| (AF197451) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 155 bits (389), Expect = 5e-37
Identities = 108/310 (34%), Positives = 162/310 (51%), Gaps = 37/310 (11%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
YR+A++ GDGIGPEV+ A ++++ + D + EF G + G + + L
Sbjct: 5 YRIAILPGDGIGPEVMREAYKILSILKDHFSLSLEIQEFNIGGSAIDQEGIALPKKTLLG 64
Query: 58 IRKMDAILFGATT------TPFNVPGYRSLIVTLRKELDLYANLR----------IIPDL 101
+ DAILFG+ P N R+ ++ LRK +L+ANLR + P
Sbjct: 65 CQNSDAILFGSVGGKRWDHLPINERPERASLLPLRKHFNLFANLRPAKLYSELQHLSPIR 124
Query: 102 SN----GKEIVIVRENTEGLYARDGIGFSDR--------AIDFRIITLEGARRIAKFAIN 149
SN G +I+ VRE T G+Y SDR A D + RIA A
Sbjct: 125 SNIIKNGFDILCVRELTGGIYFGKP---SDRVKKNNVEYAFDTEVYYDYEINRIAHLAFQ 181
Query: 150 LAKERNSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHG 208
LA+ R + + K+NVL +RE+V ++++ V + ID+ ++++KNP
Sbjct: 182 LARTRKFKVCSIDKSNVLNSSILWREVVEKVSKMYPDVHLSHLYIDNATMQIIKNPNQFD 241
Query: 209 VILTENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGA 267
V+L N+FGDIISD + GSIG++PS ++ ++ L+EP GSAPDIAGK ANPI
Sbjct: 242 VLLCSNLFGDIISDECAVITGSIGMLPSASFNEKNFGLYEPAGGSAPDIAGKNTANPIAQ 301
Query: 268 ILSAAMMLDY 277
ILS +M++ Y
Sbjct: 302 ILSLSMLVRY 311
>gb|AAG31393.1| (AF197453) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 155 bits (389), Expect = 5e-37
Identities = 108/310 (34%), Positives = 161/310 (51%), Gaps = 37/310 (11%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
YR+A++ GDGIGPEV+ A +++N + + + F EF G G + + L
Sbjct: 5 YRIAILPGDGIGPEVMREAYKILNILKNDFLLPLEFQEFNIGGIAIDNEGLALPKKTLLG 64
Query: 58 IRKMDAILFGAT------TTPFNVPGYRSLIVTLRKELDLYANLR----------IIPDL 101
DAILFG+ T P + R+ ++ LRK +L+ANLR + P
Sbjct: 65 CENADAILFGSVGGKKWDTLPIDQRPERAALLPLRKHFNLFANLRPAKLYSELEYLSPLC 124
Query: 102 S----NGKEIVIVRENTEGLY--------ARDGIGFSDRAIDFRIITLEGARRIAKFAIN 149
S NG +I+ VRE T G+Y +D I + A D + RIA A
Sbjct: 125 SKITKNGFDILCVRELTGGVYFGHPHGRVKKDDIEY---AFDTEVYYDYEINRIAHLAFQ 181
Query: 150 LAKERNSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHG 208
LA+ R + + + K+NVL ++EIV +++ V + ID+ ++++KNP
Sbjct: 182 LARNRKNKVCSIDKSNVLNSSLLWKEIVEKVSNSYSDVRLSHLYIDNATMQIIKNPNQFD 241
Query: 209 VILTENMFGDIISDLATIHAGSIGIVPSGNYGD-EIALFEPIHGSAPDIAGKGIANPIGA 267
V+L N+FGDIISD +GSIG++PS + + L+EP GSAPDI GK IANPI
Sbjct: 242 VLLCSNLFGDIISDECAAISGSIGMLPSASLNENNFGLYEPAGGSAPDIEGKNIANPIAQ 301
Query: 268 ILSAAMMLDY 277
ILS +M++ Y
Sbjct: 302 ILSVSMLVRY 311
>gb|AAG31399.1| (AF197455) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 155 bits (389), Expect = 5e-37
Identities = 108/307 (35%), Positives = 160/307 (51%), Gaps = 31/307 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
YR+AV+ GDGIGPEV+ A +++N + D + EF G + G + + L
Sbjct: 5 YRIAVLPGDGIGPEVMREAYKILNILKDDFSLPLEIKEFNIGGAAIDQEGVALPKKTLLG 64
Query: 58 IRKMDAILFGAT------TTPFNVPGYRSLIVTLRKELDLYANLRI------IPDLS--- 102
DAILFG+ P N R+ ++ LRK +L+ANLR + +LS
Sbjct: 65 CENSDAILFGSVGGKKWDNLPINQRPERASLLPLRKHFNLFANLRPAQLYSELKNLSPLR 124
Query: 103 -----NGKEIVIVRENTEGLYARDGIGFSDR-----AIDFRIITLEGARRIAKFAINLAK 152
NG I+ +RE T G+Y G + A D I RIA A L++
Sbjct: 125 SNIIKNGFNILCIRELTGGIYFGKPTGRLKKNNIEYAFDTEIYYNYEINRIAHLAFQLSQ 184
Query: 153 ERNSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVIL 211
RN + + K+NVL +REIV ++++ V + ID+ ++++K+P V+L
Sbjct: 185 TRNYKVCSIDKSNVLNSSILWREIVKKVSKHYPDVHLSHLYIDNATMQIIKDPNQFDVLL 244
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGD-EIALFEPIHGSAPDIAGKGIANPIGAILS 270
N+FGDIISD + GSIG++PS + + + L+EP GSAPDI GK IANPI ILS
Sbjct: 245 CSNLFGDIISDECAMITGSIGMLPSASLNEKKFGLYEPAGGSAPDIEGKNIANPIAQILS 304
Query: 271 AAMMLDY 277
+M++ Y
Sbjct: 305 LSMLVRY 311
>sp|Q9Y897|LEU3_MYCGR 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|5107397|gb|AAD40111.1|AF156181_1
(AF156181) 3-isopropylmalate dehydrogenase
[Mycosphaerella graminicola]
Length = 365
Score = 155 bits (388), Expect = 7e-37
Identities = 118/359 (32%), Positives = 179/359 (48%), Gaps = 43/359 (11%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFE------GGFEVFKRIGSPISEDDL 55
Y + V GD GPEV A++V++ + + F GG + G P++++ L
Sbjct: 4 YNIVVFGGDHCGPEVTAEALKVLDVIDNSNADVHFNIQPHLLGGASIDAH-GEPLTDEAL 62
Query: 56 KEIRKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------II 98
+ DA++ GA P G I+ LRKE+ Y NLR +
Sbjct: 63 AAAKAADAVILGAIGGPKWGTGKVRPEQGILRLRKEMGTYGNLRPCFFASESLVKTSPLK 122
Query: 99 PDLSNGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKE 153
++ G IVRE T G+Y DG G++ + +E R+A F + LA++
Sbjct: 123 EEVCRGVNFNIVRELTGGIYFGERTEDDGSGYAVDTEPYSRAEIERVARLAGF-LALAED 181
Query: 154 RNSFITFVHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGVI 210
+ + KANV+ R +R+ V ++ E +++ +IDS + +VKNP +GVI
Sbjct: 182 PPCPVWSLDKANVMATSRLWRKTVTDVFANEFPQLKIGHHLIDSAAMLMVKNPRALNGVI 241
Query: 211 LTENMFGDIISDLATIHAGSIGIVPSGNYGDEI-------ALFEPIHGSAPDIAGKGIAN 263
+T N+FGDIISD A++ GS+G++PS + ++EPIHGSAPDI+GKGI N
Sbjct: 242 VTSNLFGDIISDEASVIPGSLGLLPSASLTASPDGKSKCNGIYEPIHGSAPDISGKGIVN 301
Query: 264 PIGAILSAAMMLDYLGLN---GKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
P+ ILS MM Y K + EA R + G T D+GG+A T+EV I E+
Sbjct: 302 PVAMILSLGMMCKYSLQQPELAKKIDEAVRNVIEKGINTADIGGSAKTAEVGDAIAKEL 360
>gb|AAG31390.1| (AF197452) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 364
Score = 155 bits (388), Expect = 7e-37
Identities = 110/315 (34%), Positives = 167/315 (52%), Gaps = 32/315 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
YR+AV+ GDGIGPEV+ A +++N + D + EF G + G + ++ L
Sbjct: 5 YRIAVLPGDGIGPEVMQEAYKILNILKDHFLLPLEMKEFNIGGIAIDQEGIALPKNTLLG 64
Query: 58 IRKMDAILFGATT------TPFNVPGYRSLIVTLRKELDLYANLR----------IIPDL 101
DAILFG+ + R+ ++ LRK +L++NLR + P
Sbjct: 65 CENSDAILFGSVGGKKWDYLSIDKRPERAALLPLRKHFNLFSNLRPAKLYADLKYLSPLR 124
Query: 102 SN----GKEIVIVRENTEGLYARDGIGFSDR-----AIDFRIITLEGARRIAKFAINLAK 152
SN G +I+ VRE T G+Y G S + A D + RIA A LA+
Sbjct: 125 SNIIKDGFDILCVRELTGGIYFGKPNGHSKKNDIEYAFDTEVYYDYEISRIAHLAFQLAR 184
Query: 153 ERNSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVIL 211
R + + K+NVLK ++EIV +I++ V + ID+ +++++K+P VIL
Sbjct: 185 NRKKKVCSLDKSNVLKSSILWKEIVEKISKNYPDVHLSHLYIDNAIMQIIKDPSQFDVIL 244
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILS 270
N+FGDI+SD GSIG++PS + ++ L+EP GSAPDIAGK IANPI ILS
Sbjct: 245 CSNLFGDIVSDECAAITGSIGMLPSASLNEKNFGLYEPAGGSAPDIAGKNIANPIAQILS 304
Query: 271 AAMMLDYLGLNGKVV 285
+M++ + G+N K +
Sbjct: 305 VSMLVRH-GMNLKKI 318
>gb|AAB63461.1| (AF009036) NAD(+)-isocitrate dehydrogenase subunit I [Ajellomyces
capsulatus]
Length = 388
Score = 155 bits (388), Expect = 7e-37
Identities = 109/338 (32%), Positives = 176/338 (51%), Gaps = 21/338 (6%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFE-GGFEVFKRIGSPISEDDLKEIRK 60
Y V +I GDGIG EV +S + + I + + + G + + + ++ + +++
Sbjct: 52 YTVTLIPGDGIGTEVAESVKTIFKADNVPIEWEQVDVSGLDAGNKHSEDLFKESIASLKR 111
Query: 61 MDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRENT 114
L G TP G++S V LR+ELD+YA++ +I ++ K ++ I+RENT
Sbjct: 112 NKLGLKGILHTPVERSGHQSFNVALRQELDIYASIVLIKNIPGYKTRHDNVDLCIIRENT 171
Query: 115 EGLYAR-DGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK-GDR 171
EG Y+ + S +IIT + RIAKFA + A N +T +HKAN++K D
Sbjct: 172 EGEYSGLEHQSVSGVVESLKIITRAKSERIAKFAFSFALANNRKKVTCIHKANIMKLADG 231
Query: 172 FFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGS 230
FR ++AE +E + I+D+ ++ V P V++ N++G I+S++ G
Sbjct: 232 LFRSTFHKVAESYPTLETNDMIVDNASMQAVARPQQFDVMVMPNLYGGILSNVGAALVGG 291
Query: 231 IGIVPSGNYGDEIALFEP-IHGSAPDIAGKGIANPIGAILSAAMMLDYLGLN------GK 283
GIVP N G ++A+FEP DI GK ANP ILS +M+L +LGL+ K
Sbjct: 292 PGIVPGCNMGRDVAVFEPGCRHVGLDIKGKDQANPTALILSGSMLLRHLGLDEHANRISK 351
Query: 284 VVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEIYS 321
V++ V T DMGG A+T E T+ ++ ++ S
Sbjct: 352 AVYDVIGEGV---TRTRDMGGQASTHEFTRAVLDKMES 386
>emb|CAB72262.1| (AJ271154) 3-isopropylmalate dehydrogenase [Phaeosphaeria nodorum]
Length = 365
Score = 155 bits (387), Expect = 9e-37
Identities = 122/356 (34%), Positives = 181/356 (50%), Gaps = 38/356 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSA---IRVVNSVTDR-IRFYEFEGGFEVFKRIGSPISEDDLKE 57
+ + V+ GDG+GPEVV A ++VV TD F E G +P++++ L
Sbjct: 4 HNIVVLAGDGVGPEVVAEAFKILKVVEKHTDNTFNFQEHLFGGCSIDAHNNPLTDEALAA 63
Query: 58 IRKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIPD 100
+ DAI+ GA P G I+ LRKEL + NLR + +
Sbjct: 64 AKGADAIILGAVGGPKWGTGKVRPEQGILKLRKELGTFGNLRPCFFASDSLVDSSPLKAE 123
Query: 101 LSNGKEIVIVRENTEGLY--ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKERNSFI 158
G I+RE T G+Y R +A+D ++E R+ + A +LA N +
Sbjct: 124 NVRGVNFNIIRELTGGIYFGERKEDEGDGKAMDTEPYSVEEIERVVRLAGSLASAENPPV 183
Query: 159 TF--VHKANVLKGDRFFREIVLEIAEREGVEVREA--IIDSFMIKLVKNPWD-HGVILTE 213
+ KANVL R +R+ I + E ++ +IDS + ++KNP +GV++T
Sbjct: 184 PVWSLDKANVLATSRLWRKTFERIMKDEYPHLKSGTHLIDSAAMLMIKNPRALNGVVVTS 243
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNYGDEI-------ALFEPIHGSAPDIAGKGIANPIG 266
N+FGDIISD A++ GS+G++PS + G ++EPIHGSAPDIAG+GI NPI
Sbjct: 244 NLFGDIISDEASVIPGSLGLLPSASLGGIPDGKTKLNGIYEPIHGSAPDIAGQGIVNPIA 303
Query: 267 AILSAAMMLDY---LGLNGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
ILS +MM Y L + EAT+ + G T D+GGT+ T+EV I +E+
Sbjct: 304 TILSISMMFKYSLCLPELALKIDEATKITIDKGVRTGDIGGTSKTAEVGDAIAAEL 359
>emb|CAA71268.1| (Y10216) hypothetical 3-isopropylmalate dehydrogenase [Arabidopsis
thaliana] >gi|6730747|gb|AAF27137.1|AC018849_25
(AC018849) putative 3-isopropylmalate dehydrogenase;
30797-28504 [Arabidopsis thaliana]
Length = 405
Score = 154 bits (386), Expect = 1e-36
Identities = 105/312 (33%), Positives = 160/312 (50%), Gaps = 42/312 (13%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTD----RIRFYEFEGGFEVFKRIGSPISEDDLKE 57
Y + ++ GDGIGPEVV A V+ F E G +G P+ E+ +
Sbjct: 43 YTITLLPGDGIGPEVVSIAKNVLQQAGSLEGVEFNFREMPIGGAALDLVGVPLPEETISA 102
Query: 58 IRKMDAILFGATTTPFNVPGYR-----------SLIVTLRKELDLYANLR---IIPDL-- 101
++ DA+L GA + GY+ ++ +R L ++ANLR ++P L
Sbjct: 103 AKESDAVLLGA------IGGYKWDNNEKHLRPEKGLLQIRAALKVFANLRPATVLPQLVD 156
Query: 102 --------SNGKEIVIVRENTEGLYARDGIGFSDR------AIDFRIITLEGARRIAKFA 147
+ G ++++VRE T G+Y + G + + RIA+ A
Sbjct: 157 ASTLKREVAEGVDLMVVRELTGGIYFGEPRGIKTNENGEEVGFNTEVYAAHEIDRIARVA 216
Query: 148 INLAKERNSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWD 206
A++R + V KANVL+ +R+ V +A E VE+ +D+ ++LV++P
Sbjct: 217 FETARKRRGKLCSVDKANVLEASILWRKRVTALASEYPDVELSHMYVDNAAMQLVRDPKQ 276
Query: 207 HGVILTENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPI 265
I+T N+FGDI+SD A++ GSIG++PS + D LFEPIHGSAPDIAG+ ANP+
Sbjct: 277 FDTIVTNNIFGDILSDEASMITGSIGMLPSASLSDSGPGLFEPIHGSAPDIAGQDKANPL 336
Query: 266 GAILSAAMMLDY 277
ILSAAM+L Y
Sbjct: 337 ATILSAAMLLKY 348
>sp|P87256|LE3A_ASPNG 3-ISOPROPYLMALATE DEHYDROGENASE A (BETA-IPM DEHYDROGENASE A) (IMDH
A) (3-IPM-DH A) >gi|7431319|pir||S72209
3-isopropylmalate dehydrogenase (EC 1.1.1.85) A -
Aspergillus niger >gi|1911741|gb|AAB50827.1| (S83228)
beta-isopropylmalate dehydrogenase [Aspergillus niger,
strain A733, Peptide, 363 aa] >gi|4097255|gb|AAD10278.1|
(U51130) beta-isopropylmalate dehydrogenase [Aspergillus
niger]
Length = 363
Score = 154 bits (386), Expect = 1e-36
Identities = 119/356 (33%), Positives = 179/356 (49%), Gaps = 39/356 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR-----IRFYEFEGGFEVFKRIGSPISEDDLK 56
Y + V GD GPEV AI+V+ + + + G +P++++ L
Sbjct: 4 YNIVVFAGDHWGPEVTAEAIKVLRVIEKSRDDITLNLQDHLLGGASIDATANPLTDEALA 63
Query: 57 EIRKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIP 99
+ DA+L GA P G ++ +RKE+ + NLR +
Sbjct: 64 AAKNADAVLLGAIGGPKWGTGAVRPEQGLLNVRKEMGTFGNLRPCNFAAPSLVEHSPLKA 123
Query: 100 DLSNGKEIVIVRENTEGLYARDGIGFSDRA-IDFRIITLEGARRIAKFAINLAKERNSFI 158
+ G + I+RE T G+Y D + A +D + RI A +LA + N +
Sbjct: 124 SVCEGVDFNIIRELTGGIYFGDRKKMTAAATMDTEPYSRAEIERITPRA-HLALQHNPPL 182
Query: 159 TF--VHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGVILTE 213
+ KANVL R +R+ V EI +E +++ +IDS + +VKNP +G+I+T
Sbjct: 183 PVWSLDKANVLATSRLWRKTVTEIMAKEFPQLKIEHQLIDSAAMIMVKNPRQLNGIIVTS 242
Query: 214 NMFGDIISDLATIHAGSIGIVPSGNY-------GDEIALFEPIHGSAPDIAGKGIANPIG 266
N+FGDIISD A++ GS+G++PS + G ++EPIHGSAPDIAGKGI NP+
Sbjct: 243 NLFGDIISDEASVIPGSLGLLPSASLSGIPDGKGRVNGIYEPIHGSAPDIAGKGIVNPVA 302
Query: 267 AILSAAMMLDY-LGL--NGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
AILS AMM+ Y G + + A R + G T D+GG ATTSEV + +E+
Sbjct: 303 AILSVAMMMQYSFGRFDEARAIEAAVRNVLESGVRTGDIGGKATTSEVGDAVAAEL 358
>sp|O77784|IDHB_BOVIN ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT BETA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
(NAD(+)-ISOCITRATE DEHYDROGENASE SUBUNIT 1) (IDH1)
>gi|3643261|gb|AAC83167.1| (AF090322) NAD(+)-isocitrate
dehydrogenase subunit 1 IDH1-B precursor [Bos taurus]
Length = 385
Score = 154 bits (385), Expect = 2e-36
Identities = 107/336 (31%), Positives = 180/336 (52%), Gaps = 16/336 (4%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKM 61
+ V ++ GDG+GPE++ + V + + + F E EV E L +++
Sbjct: 49 FPVTMLPGDGVGPELMHAVKEVFKAASVPVEFQEHHLS-EVQNMASEEKLEQVLSSMKEN 107
Query: 62 DAILFGATTTPFNVPG-YRSLIVTLRKELDLYANLRIIPDLS------NGKEIVIVRENT 114
+ G TP G S + LR++LDL+AN+ + L N ++VI+RE T
Sbjct: 108 KVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYKTRHNNLDLVIIREQT 167
Query: 115 EGLYARDGIGFSDRAID-FRIITLEGARRIAKFAINLA-KERNSFITFVHKANVLK-GDR 171
EG Y+ + I+ +I+T ++RIAKFA + A K+ +T VHKAN++K GD
Sbjct: 168 EGEYSSLEHESARGVIECLKIVTRTKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDG 227
Query: 172 FFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGS 230
F + E+AE ++ + IID+ ++LV+NP+ V++ N++G+II +LA G
Sbjct: 228 LFLQCCEEVAELYPKIKFEKMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGG 287
Query: 231 IGIVPSGNYGDEIALFEP-IHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVVWE 287
G+VP +Y E A+FE G+ IANP +LSA+ ML +L L + ++ E
Sbjct: 288 AGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEHHSNMIAE 347
Query: 288 ATRRYVRYGNL-TPDMGGTATTSEVTKGIISEIYSF 322
A ++ ++ G + T DMGG +TT++ K +I ++ +
Sbjct: 348 AVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHLHPY 383
>sp|P41766|LEU3_KLUMA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|479255|pir||S32969 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - yeast (Kluyveromyces
marxianus) >gi|2700|emb|CAA43710.1| (X61490)
3-isopropylmalate dehydrogenase [Kluyveromyces
marxianus]
Length = 359
Score = 153 bits (384), Expect = 2e-36
Identities = 118/359 (32%), Positives = 182/359 (49%), Gaps = 52/359 (14%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTD-----RIRFYEFEGGFEVFKRIGSPISEDDLKEI 58
+ V+ GD +G E+ + AI+V+N++++ + F G G P+ ++ K
Sbjct: 5 IVVLPGDHVGTEITNEAIKVLNAISEARPSIKFNFEHHLIGGAAIDATGVPLPDEASK-- 62
Query: 59 RKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIPDL 101
K DA+L GA P G ++ +RKEL LYANLR + P+
Sbjct: 63 -KADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELGLYANLRPCNFASDSLLDLSPLKPEY 121
Query: 102 SNGKEIVIVRENTEGLY-------ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKER 154
+ G + V+VRE G+Y DG+ + D ++ +RI + A LA +
Sbjct: 122 AKGTDFVVVRELVGGIYFGERKEDEGDGVAW-----DSEKYSVPEVQRITRMAAFLALQH 176
Query: 155 NSFITF--VHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGV 209
N + + KANVL R +R+ V E + E + V+ +IDS + LVK+P +G+
Sbjct: 177 NPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPQLTVQHQLIDSAAMILVKSPTKLNGI 236
Query: 210 ILTENMFGDIISDLATIHAGSIGIVPSGNYGD------EIALFEPIHGSAPDIAGKGIAN 263
++T NMFGDIISD A++ GS+G++PS + L+EP HGSAPD+ + N
Sbjct: 237 VITNNMFGDIISDEASVIPGSLGLLPSASLASLPDTNKAFGLYEPCHGSAPDLPANKV-N 295
Query: 264 PIGAILSAAMMLDY---LGLNGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
PI ILSAAMML L G+ V EA R+ + G T D+GG+ +T+EV + +
Sbjct: 296 PIATILSAAMMLKLSLDLVEEGRAVEEAVRKVLDSGIRTGDLGGSNSTTEVGDAVAKAV 354
>sp|P08791|LEU3_PICJA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|482489|pir||A47620 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - yeast (Pichia jadinii)
>gi|170874|gb|AAA34347.1| (M16014) 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) [Candida utilis]
Length = 363
Score = 153 bits (384), Expect = 2e-36
Identities = 117/357 (32%), Positives = 179/357 (49%), Gaps = 45/357 (12%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTD-----RIRFYEFEGGFEVFKRIGSPISEDDLKEI 58
+ V+ GD +G E+ AI+V+ ++ + + F G G P+ +D L+
Sbjct: 6 IVVLPGDHVGTEITAEAIKVLKAIEEVKPEIKFNFQHHLIGGAAIDATGVPLPDDALEAS 65
Query: 59 RKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIPDL 101
+K DA+L GA P G ++ +RKEL+LYANLR I ++
Sbjct: 66 KKADAVLLGAVGGPKWGTGAVRPEQGLLKIRKELNLYANLRPCNFASESLLDLSPIKAEV 125
Query: 102 SNGKEIVIVRENTEGLY-----ARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKERNS 156
G + V+VRE G+Y DG G A D ++ +RI + A +A + N
Sbjct: 126 VKGTDFVVVRELVGGIYFGERKEDDGSGV---ASDTETYSVPEVQRITRMAAFMALQHNP 182
Query: 157 FITF--VHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-HGVIL 211
+ + KANVL R +R++V E E+E + V+ +IDS + L+K P +G+++
Sbjct: 183 PLPIWSLDKANVLASSRLWRKVVTETIEKEFPQLTVQHQLIDSAAMILIKYPTQLNGIVI 242
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGD------EIALFEPIHGSAPDIAGKGIANPI 265
T NMFGDIISD A++ GS+G++PS + L+EP HGSAPD+ + NPI
Sbjct: 243 TSNMFGDIISDEASVIPGSLGLLPSASLASLPDSNKAFGLYEPCHGSAPDLPANKV-NPI 301
Query: 266 GAILSAAMMLDY---LGLNGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIISEI 319
ILSAAMML L G V A ++ + G T D+ GT +T+EV + +
Sbjct: 302 ATILSAAMMLKLSLDLYEEGVAVETAVKQVLDAGIRTGDLKGTNSTTEVGDAVAEAV 358
>sp|P48572|LEU3_BUCRP 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|899389|emb|CAA50616.1| (X71612)
isopropylmalate dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 153 bits (384), Expect = 2e-36
Identities = 106/305 (34%), Positives = 158/305 (51%), Gaps = 31/305 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
+R+AV+ GDGIGPE++ A +++N + + EF G R G + E+ L+
Sbjct: 5 FRIAVLPGDGIGPEIMREAYKILNILEKNFSLPLEIREFSVGGIAIDREGIALPEETLRG 64
Query: 58 IRKMDAILFGATT------TPFNVPGYRSLIVTLRKELDLYANLR---IIPDLSN----- 103
DAILFG+ P R+ ++ LRK +L++NLR + DL N
Sbjct: 65 CENSDAILFGSVGGKKWDYLPIESRPERASLLPLRKHFNLFSNLRPAKLYSDLKNLSPLR 124
Query: 104 ------GKEIVIVRENTEGLYARDGIGFS-----DRAIDFRIITLEGARRIAKFAINLAK 152
G +I+ VRE T G+Y G S + + D I +RIA A LA
Sbjct: 125 SNIVRNGFDILCVRELTGGIYFGKPKGRSKNENGEYSFDTEIYYDFEIKRIAHLAFELAS 184
Query: 153 ERNSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVIL 211
R + + K+NVL +REIV E+++ + + ID+ ++++KNP V+L
Sbjct: 185 SRRCKVCSIDKSNVLASSILWREIVEEVSKNYPNIILSHLYIDNACMQIIKNPSQFDVLL 244
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGD-EIALFEPIHGSAPDIAGKGIANPIGAILS 270
N+FGDIISD + GSIG++PS + + L+EP GSAPDI GK IANPI ILS
Sbjct: 245 CSNIFGDIISDECAMITGSIGMLPSASLNSKKFGLYEPAGGSAPDIEGKNIANPIAQILS 304
Query: 271 AAMML 275
+M++
Sbjct: 305 LSMLI 309
>emb|CAC09449.1| (AL442090) hypothetical protein [Homo sapiens]
Length = 288
Score = 153 bits (382), Expect = 3e-36
Identities = 100/273 (36%), Positives = 155/273 (56%), Gaps = 15/273 (5%)
Query: 65 LFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRENTEGLY 118
L G TP G+ S+ + LRK DLYAN+R + K IV +RENTEG Y
Sbjct: 17 LKGPLKTPI-AAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEY 75
Query: 119 ARDGIGFSDRAID-FRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK-GDRFFRE 175
+ D + ++IT ++RIA+FA A+ + S +T VHKAN+++ D F +
Sbjct: 76 SGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQ 135
Query: 176 IVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGSIGIV 234
E+AE + ++ E +D+ + +V++P V++ N++GDI+SDL G +G+
Sbjct: 136 KCREVAESCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVT 195
Query: 235 PSGNYG-DEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVVWEATRR 291
PSGN G + +A+FE +HG+APDIAGK +ANP +LSA MML ++GL + + A
Sbjct: 196 PSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARIEAACFA 255
Query: 292 YVRYG-NLTPDMGGTATTSEVTKGIISEIYSFD 323
++ G +LT D+GG A S+ T+ I + D
Sbjct: 256 TIKDGKSLTKDLGGNAKCSDFTEEICRRVKDLD 288
>gi|11431719 isocitrate dehydrogenase 3 (NAD+) alpha [Homo sapiens]
Length = 316
Score = 153 bits (382), Expect = 3e-36
Identities = 100/273 (36%), Positives = 155/273 (56%), Gaps = 15/273 (5%)
Query: 65 LFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVRENTEGLY 118
L G TP G+ S+ + LRK DLYAN+R + K IV +RENTEG Y
Sbjct: 45 LKGPLKTPI-AAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEY 103
Query: 119 ARDGIGFSDRAID-FRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK-GDRFFRE 175
+ D + ++IT ++RIA+FA A+ + S +T VHKAN+++ D F +
Sbjct: 104 SGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQ 163
Query: 176 IVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGSIGIV 234
E+AE + ++ E +D+ + +V++P V++ N++GDI+SDL G +G+
Sbjct: 164 KCREVAESCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVT 223
Query: 235 PSGNYG-DEIALFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVVWEATRR 291
PSGN G + +A+FE +HG+APDIAGK +ANP +LSA MML ++GL + + A
Sbjct: 224 PSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARIEAACFA 283
Query: 292 YVRYG-NLTPDMGGTATTSEVTKGIISEIYSFD 323
++ G +LT D+GG A S+ T+ I + D
Sbjct: 284 TIKDGKSLTKDLGGNAKCSDFTEEICRRVKDLD 316
>sp|P29102|LEU3_BRANA 3-ISOPROPYLMALATE DEHYDROGENASE PRECURSOR (BETA-IPM DEHYDROGENASE)
(IMDH) (3-IPM-DH) >gi|81676|pir||S20510
3-isopropylmalate dehydrogenase (EC 1.1.1.85) precursor
- rape >gi|17827|emb|CAA42596.1| (X59970)
3-isopropylmalate dehydrogenase [Brassica napus]
Length = 406
Score = 152 bits (380), Expect = 6e-36
Identities = 106/312 (33%), Positives = 161/312 (50%), Gaps = 42/312 (13%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTD----RIRFYEFEGGFEVFKRIGSPISEDDLKE 57
Y + ++ GDGIGPEV+ A V+ F E G +G P+ E+ +
Sbjct: 44 YNITLLPGDGIGPEVISIAKNVLQQAGSLEGLEFSFQEMPVGGAALDLVGVPLPEETVSA 103
Query: 58 IRKMDAILFGATTTPFNVPGYR-----------SLIVTLRKELDLYANLR---IIPDL-- 101
++ DA+L GA + GY+ + ++ LR L ++ANLR ++P L
Sbjct: 104 AKESDAVLLGA------IGGYKWDKNEKHLKPETGLLQLRAGLKVFANLRPATVLPQLVD 157
Query: 102 --------SNGKEIVIVRENTEGLY--ARDGIGFSDRAIDF----RIITLEGARRIAKFA 147
+ G ++++VRE T G+Y GI ++ + + RIA+ A
Sbjct: 158 ASTLKREVAEGVDLMVVRELTGGIYFGVPRGIKTNENGEEVGYNTEVYAAHEIDRIARVA 217
Query: 148 INLAKERNSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWD 206
A++R + V KANVL +R V +A E VE+ +D+ ++LV++P
Sbjct: 218 FETARKRRGKLCSVDKANVLDASILWRRRVTALAAEYPDVELSHMYVDNAAMQLVRDPKQ 277
Query: 207 HGVILTENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPI 265
I+T N+FGDI+SD A++ GSIG++PS + D LFEPIHGSAPDIAG+ ANP+
Sbjct: 278 FDTIVTNNIFGDILSDEASMITGSIGMLPSASLSDSGPGLFEPIHGSAPDIAGQDKANPL 337
Query: 266 GAILSAAMMLDY 277
ILSAAM+L Y
Sbjct: 338 ATILSAAMLLKY 349
>sp|P41019|LEU3_BACME 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|2117493|pir||I40226 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Bacillus megaterium
>gi|414097|emb|CAA46295.1| (X65184)
3-isopropylmalat-dehydrogenase [Bacillus megaterium]
>gi|1095455|prf||2109196A 3-isopropylmalate
dehydrogenase [Bacillus megaterium]
Length = 367
Score = 152 bits (380), Expect = 6e-36
Identities = 122/354 (34%), Positives = 182/354 (50%), Gaps = 42/354 (11%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIRF-YEFEGGF---EVFKRIGSPISEDDLKEI 58
+VAV+ GDG G R+ ++ +R+R +EF G GS S ++
Sbjct: 4 KVAVLPGDG-GQSNQRRCCRL-KAIGERLRHQFEFTYGLIGGAAIDEAGSSSSRSTIETC 61
Query: 59 RKMDAILFGATTTPF--NVPGY---RSLIVTLRKELDLYANLRIIP-------------D 100
+ DA+L G+ P P + ++ +RKELDLYANLR + +
Sbjct: 62 NEADAVLLGSVGGPKWDRFPSHLRPEKGLLAIRKELDLYANLRPVTFYDSLADASPLKKE 121
Query: 101 LSNGKEIVIVRENTEGLYA-----RDGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN 155
G + +IVRE T GLY R G + +D RI +I+ A R
Sbjct: 122 YIEGVDFIIVRELTGGLYFGKPSDRRTEGNKETVVDTLFYKRTEIERIIA-SIDTAVNRR 180
Query: 156 SFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTEN 214
+T V KANVL+ R +RE+ E+A + VE+ ++DS ++L++NP V++TEN
Sbjct: 181 KKVTSVDKANVLESSRVWREVAEEVAKDYPDVELEHMLVDSAAMQLIRNPKYFDVVVTEN 240
Query: 215 MFGDIISDLATIHAGSIGIVPSGNY-GDEIALFEPIHGSAPDIAGKGIANPI-GAILSAA 272
MFGDI+SD A++ GS+G++PS + D +L+EP+HGSAPDIAG+ ANPI AILSAA
Sbjct: 241 MFGDILSDEASMLTGSLGMLPSASLTADGPSLYEPVHGSAPDIAGQNKANPIAAAILSAA 300
Query: 273 MMLDY---LGLNGKVVWEATRRYVRYGNLTPDMG------GTATTSEVTKGIIS 317
M+L + L V+ +A + G+ T D+ GT EV +I+
Sbjct: 301 MLLRHSFGLEKEAAVIEQAVESVLHAGHRTADLADGNHYLGTDKMVEVITAVIA 354
>gb|AAC83166.1| (AF090321) NAD(+)-isocitrate dehydrogenase subunit 1 IDH1-A
precursor [Bos taurus]
Length = 383
Score = 152 bits (379), Expect = 8e-36
Identities = 107/333 (32%), Positives = 177/333 (53%), Gaps = 16/333 (4%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKM 61
+ V ++ GDG+GPE++ + V + + + F E EV E L +++
Sbjct: 49 FPVTMLPGDGVGPELMHAVKEVFKAASVPVEFQEHHLS-EVQNMASEEKLEQVLSSMKEN 107
Query: 62 DAILFGATTTPFNVPG-YRSLIVTLRKELDLYANLRIIPDLS------NGKEIVIVRENT 114
+ G TP G S + LR++LDL+AN+ + L N ++VI+RE T
Sbjct: 108 KVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYKTRHNNLDLVIIREQT 167
Query: 115 EGLYARDGIGFSDRAID-FRIITLEGARRIAKFAINLA-KERNSFITFVHKANVLK-GDR 171
EG Y+ + I+ +I+T ++RIAKFA + A K+ +T VHKAN++K GD
Sbjct: 168 EGEYSSLEHESARGVIECLKIVTRTKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDG 227
Query: 172 FFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGS 230
F + E+AE ++ + IID+ ++LV+NP+ V++ N++G+II +LA G
Sbjct: 228 LFLQCCEEVAELYPKIKFEKMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGG 287
Query: 231 IGIVPSGNYGDEIALFEP-IHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVVWE 287
G+VP +Y E A+FE G+ IANP +LSA+ ML +L L + ++ E
Sbjct: 288 AGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEHHSNMIAE 347
Query: 288 ATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
A ++ ++ G + T DMGG AT + T+ +I +
Sbjct: 348 AVKKVIKVGKVRTSDMGGYATCQDFTEAVIGAL 380
>gb|AAG31396.1| (AF197454) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 365
Score = 152 bits (379), Expect = 8e-36
Identities = 106/317 (33%), Positives = 163/317 (50%), Gaps = 34/317 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
YR++V+ GDGIGPEV+ A +++N + + + EF G + G + + L
Sbjct: 5 YRISVLPGDGIGPEVMREAYKILNILKNHFSLPLEIKEFNIGGAAIDQEGVALPKKTLLG 64
Query: 58 IRKMDAILFGATT------TPFNVPGYRSLIVTLRKELDLYANLR----------IIP-- 99
DAILFG+ P + R+ ++ LRK +L+ANLR + P
Sbjct: 65 CENSDAILFGSVGGKKWDHLPIDKRPERASLLPLRKHFNLFANLRPAQLYSELKYLSPLR 124
Query: 100 ---DLSNGKEIVIVRENTEGLYARDGIGFS------DRAIDFRIITLEGARRIAKFAINL 150
+ NG I+ +RE T G+Y G + A D I RIA A L
Sbjct: 125 SDIXIKNGFNILCIRELTGGIYFGKPTGRXLKKNNIEYAFDTEIYYDYEINRIAHLAFQL 184
Query: 151 AKERNSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGV 209
A+ RN + + K+NVL +RE V ++++ V + ID+ ++++K+P +
Sbjct: 185 AQTRNYKVCSIDKSNVLNSSVLWRETVQKVSKNYPDVHLSHLYIDNATMQIIKDPNQFDI 244
Query: 210 ILTENMFGDIISDLATIHAGSIGIVPSGNYGD-EIALFEPIHGSAPDIAGKGIANPIGAI 268
+L N+FGDI+SD I GSIG++PS + + + L+EP GSAPDI GK IANPI I
Sbjct: 245 LLCSNLFGDIVSDECAIITGSIGMLPSASLNEKKFGLYEPAGGSAPDIEGKNIANPIAQI 304
Query: 269 LSAAMMLDYLGLNGKVV 285
LS +M++ Y G+N K +
Sbjct: 305 LSVSMLVRY-GMNLKKI 320
>sp|P56933|LEU3_BUCAI 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|5912537|emb|CAB56191.1| (AJ006878)
3-isopropylmalate dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 151 bits (378), Expect = 1e-35
Identities = 103/311 (33%), Positives = 164/311 (52%), Gaps = 32/311 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTD----RIRFYEFEGGFEVFKRIGSPISEDDLKE 57
YR+AV+ GDGIGPEV+ A +++N + + +F G +R G + + L
Sbjct: 5 YRIAVLSGDGIGPEVMQEACKILNVLKKYFFLSLEIQKFNIGGIAIEREGVALPKTTLLG 64
Query: 58 IRKMDAILFGAT------TTPFNVPGYRSLIVTLRKELDLYANLR---IIPDLS------ 102
D+IL G+ P R+ ++ LRK +L++NLR + P+L
Sbjct: 65 CENSDSILLGSVGGKKWDNLPVEQRPERAALLPLRKYFNLFSNLRPAKLYPELKCLSPLR 124
Query: 103 -----NGKEIVIVRENTEGLYARDGIGFSDR-----AIDFRIITLEGARRIAKFAINLAK 152
NG +I+ VRE T G+Y + GF ++ A D I RIA A LA+
Sbjct: 125 SDIVKNGFDILCVRELTGGIYFGEPKGFVNKNNTKYAFDTEIYHEYEIIRIAHLAFKLAR 184
Query: 153 ERNSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVIL 211
+ + + K+NVL+ + ++V ++++ V + ID+ ++++K+P V+L
Sbjct: 185 SKKKKVCSIDKSNVLRSSILWGKVVESVSKKYPDVHLSHLYIDNAAMQIIKDPNQFDVLL 244
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILS 270
N+FGDIISD GSIG++PS ++ ++ L+EP GSAPDI GK IANPI ILS
Sbjct: 245 CSNLFGDIISDECATITGSIGMLPSASFNEKNFGLYEPAGGSAPDIEGKNIANPIAQILS 304
Query: 271 AAMMLDYLGLN 281
+M++ Y G+N
Sbjct: 305 LSMLVRY-GMN 314
>sp|Q12545|LEU3_CEPAC 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|840666|dbj|BAA09319.1| (D50665)
beta-isopropylmalate dehydrogenase [Acremonium
chrysogenum]
Length = 380
Score = 151 bits (377), Expect = 1e-35
Identities = 119/361 (32%), Positives = 184/361 (50%), Gaps = 53/361 (14%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSV-TDRIRFY-----EFEGGFEVFKRIGSPISEDDL 55
Y++ V+ GD IGPE++ AI+V+ ++ T R + + GG + G PI++ L
Sbjct: 5 YKILVLPGDHIGPEIMAEAIKVLTTIETHRPNLHFNLTTDLVGGTSIDTH-GVPITQSVL 63
Query: 56 KEIRKMDAILFGATTTPFNV---PGYRSLIVTLRKELDLYANLR----IIPDLSN----- 103
+ DA+LFG+ P P S ++ LR+ LD +ANLR ++P L
Sbjct: 64 DAAKASDAVLFGSIGGPEWAGVHPTPESGLLQLRQHLDAFANLRPCEFLVPSLVGASPIR 123
Query: 104 -----GKEIVIVRENTEGLYARDGIGFSDRAIDFRIITLEGARRIAKFAINLAK------ 152
G ++VREN G Y + D A D + T R+A+ + +A+
Sbjct: 124 EHVVKGTRFIVVRENCGGAYFGEKKEEEDVASDLWVYTRPEIERLARVSAAVARIMGRSE 183
Query: 153 --ERNSFITFVHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWD-H 207
+ + + KANVL R +R I +I +E + ++ + DS + +V++P +
Sbjct: 184 DDNQAATVWSADKANVLASGRLWRRITSDIFAKEFPDITLQHQLADSMAMLMVRDPRRFN 243
Query: 208 GVILTENMFGDIISDLATIHAGSIGIVPS-------GNYGDEIALFEPIHGSAPDIAGKG 260
GVI T+N FGDI+SD++ G++G++PS G ++EP+HGSAPDI+GKG
Sbjct: 244 GVIHTDNTFGDILSDISGAITGTLGLMPSASLCGVPGEGHRSNGIYEPVHGSAPDISGKG 303
Query: 261 IANPIGAILSAAMMLDY-LGLNGKVVWEATRRYVRY------GNL---TPDMGGTATTSE 310
+ANP+ ILS AMML Y +GL K R V+ G L T D+GG AT S+
Sbjct: 304 LANPVAQILSVAMMLRYSMGLE-KEATAVERAVVKVLDAKSEGGLEIRTGDLGGRATCSQ 362
Query: 311 V 311
V
Sbjct: 363 V 363
>sp|Q28479|IDHB_MACFA ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT BETA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|2134531|pir||S58663 isocitrate dehydrogenase (NAD+)
(EC 1.1.1.41) beta chain - crab-eating macaque
(fragment) >gi|1182009|emb|CAA57954.1| (X82632)
NAD+-isocitrate dehydrogenase [Macaca fascicularis]
Length = 381
Score = 151 bits (377), Expect = 1e-35
Identities = 107/333 (32%), Positives = 177/333 (53%), Gaps = 16/333 (4%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKM 61
+ V ++ GDG+GPE++ + V + + F E EV E L +++
Sbjct: 47 FPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLS-EVQNMASEEKLEQVLSSMKEN 105
Query: 62 DAILFGATTTPFNVPG-YRSLIVTLRKELDLYANLRIIPDLS------NGKEIVIVRENT 114
+ G TP G S + LR++LDL+AN+ + L N ++VI+RE T
Sbjct: 106 KVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQT 165
Query: 115 EGLYARDGIGFSDRAID-FRIITLEGARRIAKFAINLA-KERNSFITFVHKANVLK-GDR 171
EG Y+ + I+ +I+T ++RIAKFA + A K+ S +T VHKAN++K GD
Sbjct: 166 EGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRSKVTAVHKANIMKLGDG 225
Query: 172 FFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGS 230
F + E+AE ++ IID+ ++LV+NP+ V++ N++G+II +LA G
Sbjct: 226 LFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGG 285
Query: 231 IGIVPSGNYGDEIALFEP-IHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVVWE 287
G+VP +Y E A+FE G+ IANP +LSA+ ML +L L + ++ +
Sbjct: 286 AGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSNMIAD 345
Query: 288 ATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
A ++ ++ G + T DMGG AT + T+ +I+ +
Sbjct: 346 AVKKVIKVGKVRTSDMGGYATCHDFTEAVIAAL 378
>gb|AAB81675.1| (AC002354) putative NAD+ dependent isocitrate dehydrogenase subunit
2, IDH2 [Arabidopsis thaliana]
Length = 367
Score = 151 bits (377), Expect = 1e-35
Identities = 116/339 (34%), Positives = 179/339 (52%), Gaps = 35/339 (10%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKMDA 63
V +I GDG+GP V ++ +V+ ++ + F FE ++ + E L+ I+K
Sbjct: 40 VTLIPGDGVGPLVTNAVQQVMEAMHAPVYFEPFEVHGDM-----KSLPEGLLESIKKNKV 94
Query: 64 ILFGATTTPFNVPGYRSLIVTLRKELDLYANLRI---IPDLSNGKE---IVIVRENTEGL 117
L G TP G SL V LRKELDL+A+L +P L++ E IV++RENTEG
Sbjct: 95 CLKGGLKTPVG-GGVSSLNVNLRKELDLFASLVNCFNLPGLASRHENVDIVVIRENTEGE 153
Query: 118 YARDGIGFSDRAI-----DFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK-GD 170
YA G + ++IT + RIAK+A A N +T VHKAN++K D
Sbjct: 154 YA----GLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLAD 209
Query: 171 RFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAG 229
F E E+A++ + E I+D+ ++LV P V++T N++G+++++ A AG
Sbjct: 210 GLFLESCQEVAKKYPSIAYNEIIVDNCCMQLVARPEQFDVMVTPNLYGNLVANTAAGIAG 269
Query: 230 SIGIVPSGNYGDEIALFEPIHGSAPDIAGKGI------ANPIGAILSAAMMLDYLGLNGK 283
G++P GN G E A+FE G++ GK ANP+ +LS+AMML +L
Sbjct: 270 GTGVMPGGNVGAEYAVFE--QGASAGNVGKDTTEEQKNANPVALLLSSAMMLRHLQFPSF 327
Query: 284 V--VWEATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
+ A +R + GN T D+GG +TT EV +I+ +
Sbjct: 328 ADRLETAVKRVIAEGNCRTEDLGGNSTTQEVVDAVIANL 366
>sp|Q93714|IDHA_CAEEL PROBABLE ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA,
MITOCHONDRIAL PRECURSOR (ISOCITRIC DEHYDROGENASE)
(NAD+-SPECIFIC ICDH) >gi|7503330|pir||T22149
hypothetical protein F43G9.1 - Caenorhabditis elegans
>gi|3877109|emb|CAB02111.1| (Z79755) predicted using
Genefinder~Similarity to Bovine isocitrate dehydrogenase
(SW:IDH3_BOVIN), contains similarity to Pfam domain:
PF00180 (Isocitrate and isopropylmalate dehydrogenases),
Score=460.9, E-value=2.1e-137, N=1~cDNA EST EMBL:Z14736
come>
Length = 374
Score = 151 bits (377), Expect = 1e-35
Identities = 119/355 (33%), Positives = 177/355 (49%), Gaps = 48/355 (13%)
Query: 3 RVAVIKGDGIGPEVVDSAIRVVNSVT-----DRIRFYEFEGGFEVFKRIGSPISEDDLKE 57
RV +I GDGIGPE+ S ++ + D + +G VF RI S E L
Sbjct: 26 RVTLIPGDGIGPEISASVQKIFEAADAPIAWDPVDVTPVKGRDGVF-RIPSRCIE--LMH 82
Query: 58 IRKMDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK------EIVIVR 111
K+ L G TP G+RSL + +RKE LYAN+R L K ++V +R
Sbjct: 83 ANKVG--LKGPLETPIG-KGHRSLNLAVRKEFSLYANVRPCRSLEGHKTLYDNVDVVTIR 139
Query: 112 ENTEGLYARDGIGFSDRAI-----DFRIITLEGARRIAKFAINLAKERN-SFITFVHKAN 165
ENTEG Y+ G + ++IT +R +A FA A++ +T VHKAN
Sbjct: 140 ENTEGEYS----GIEHEIVPGVVQSIKLITETASRNVASFAFEYARQNGRKVVTAVHKAN 195
Query: 166 VLK-GDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDL 223
+++ D F I E A ++ +EA +D+ + +V++P + V++ N++GDI+SDL
Sbjct: 196 IMRQSDGLFLSICREQAALYPDIKFKEAYLDTVCLNMVQDPSQYDVLVMPNLYGDILSDL 255
Query: 224 ATIHAGSIGIVPSGNYG------DEIA----------LFEPIHGSAPDIAGKGIANPIGA 267
G +G+ PSGN G DEI L+ +HG+APDIAG+ ANP
Sbjct: 256 CAGLVGGLGVTPSGNIGKGAAVFDEIQFFIQTKLTKFLYRSVHGTAPDIAGQDKANPTAL 315
Query: 268 ILSAAMMLDYLGL--NGKVVWEATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
+LSA MML Y+ L + + +A + G T D+GGT T S T + + +
Sbjct: 316 LLSAVMMLRYMNLPQHAARIEKAVFDAIADGRAKTGDLGGTGTCSSFTADVCARV 370
>gb|AAC49965.1| (U81994) NAD+ dependent isocitrate dehydrogenase subunit 2
[Arabidopsis thaliana]
Length = 367
Score = 150 bits (376), Expect = 2e-35
Identities = 116/339 (34%), Positives = 179/339 (52%), Gaps = 35/339 (10%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKMDA 63
V +I GDG+GP V ++ +V+ ++ + F FE ++ + E L+ I+K
Sbjct: 40 VTLIPGDGVGPLVTNAVQQVMEAMHAPVYFEPFEVHGDM-----KSLPEGLLESIKKNKV 94
Query: 64 ILFGATTTPFNVPGYRSLIVTLRKELDLYANLRI---IPDLSNGKE---IVIVRENTEGL 117
L G TP G SL V LRKELDL+A+L +P L++ E IV++RENTEG
Sbjct: 95 CLKGGLKTPVG-GGVSSLNVNLRKELDLFASLVNCFNLPGLASRHENVDIVVIRENTEGE 153
Query: 118 YARDGIGFSDRAI-----DFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK-GD 170
YA G + ++IT + RIAK+A A N +T VHKAN++K D
Sbjct: 154 YA----GLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLAD 209
Query: 171 RFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAG 229
F E E+A++ + E I+D+ ++LV P V++T N++G+++++ A AG
Sbjct: 210 GLFLESCQEVAKKYPSIAYNEIIVDNCCMQLVARPEQFDVMVTPNLYGNLVANTAAGIAG 269
Query: 230 SIGIVPSGNYGDEIALFEPIHGSAPDIAGKGI------ANPIGAILSAAMMLDYLGLNGK 283
G++P GN G E A+FE G++ GK ANP+ +LS+AMML +L
Sbjct: 270 GSGVMPGGNVGAEYAVFE--QGASAGNVGKDTTEEQKNANPVALLLSSAMMLRHLQFPSF 327
Query: 284 V--VWEATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
+ A +R + GN T D+GG +TT EV +I+ +
Sbjct: 328 ADRLETAVKRVIAEGNCRTEDLGGNSTTQEVVDAVIANL 366
>gi|5901982 isocitrate dehydrogenase 3 (NAD+) beta [Homo sapiens]
>gi|6166246|sp|O43837|IDHB_HUMAN ISOCITRATE
DEHYDROGENASE [NAD] SUBUNIT BETA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|2737886|gb|AAB94295.1| (U49283) NAD+-specific
isocitrate dehydrogenase beta precursor [Homo sapiens]
Length = 385
Score = 150 bits (375), Expect = 2e-35
Identities = 106/333 (31%), Positives = 176/333 (52%), Gaps = 16/333 (4%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKM 61
+ V ++ GDG+GPE++ + V + + F E EV E L +++
Sbjct: 49 FPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLS-EVQNMASEEKLEQVLSSMKEN 107
Query: 62 DAILFGATTTPFNVPG-YRSLIVTLRKELDLYANLRIIPDLS------NGKEIVIVRENT 114
+ G TP G S + LR++LDL+AN+ + L N ++VI+RE T
Sbjct: 108 KVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQT 167
Query: 115 EGLYARDGIGFSDRAID-FRIITLEGARRIAKFAINLA-KERNSFITFVHKANVLK-GDR 171
EG Y+ + I+ +I+T ++RIAKFA + A K+ +T VHKAN++K GD
Sbjct: 168 EGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDG 227
Query: 172 FFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGS 230
F + E+AE ++ IID+ ++LV+NP+ V++ N++G+II +LA G
Sbjct: 228 LFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGG 287
Query: 231 IGIVPSGNYGDEIALFEP-IHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVVWE 287
G+VP +Y E A+FE G+ IANP +LSA+ ML +L L + ++ +
Sbjct: 288 AGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIAD 347
Query: 288 ATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
A ++ ++ G + T DMGG +TT++ K +I +
Sbjct: 348 AVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHL 380
>pir||T06131 isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) chain 1 - Arabidopsis
thaliana >gi|3080424|emb|CAA18743.1| (AL022604) NAD+
dependent isocitrate dehydrogenase subunit 1
[Arabidopsis thaliana] >gi|7270478|emb|CAB80243.1|
(AL161587) NAD+ dependent isocitrate dehydrogenase
subunit 1 [Arabidopsis thaliana]
Length = 367
Score = 150 bits (375), Expect = 2e-35
Identities = 114/339 (33%), Positives = 179/339 (52%), Gaps = 35/339 (10%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKMDA 63
V +I GDGIGP V ++ +V+ ++ I F +++ E+ S + + ++ IRK
Sbjct: 40 VTLIPGDGIGPLVTNAVEQVMEAMHAPIFFEKYDVHGEM-----SRVPPEVMESIRKNKV 94
Query: 64 ILFGATTTPFNVPGYRSLIVTLRKELDLYA------NLRIIPDLSNGKEIVIVRENTEGL 117
L G TP G SL V LRKELDL+A NL +P +IV++RENTEG
Sbjct: 95 CLKGGLKTPVG-GGVSSLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGE 153
Query: 118 YARDGIGFSDRAI-----DFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK-GD 170
YA G + ++IT + RIAK+A A N +T VHKAN++K D
Sbjct: 154 YA----GLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLAD 209
Query: 171 RFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAG 229
F E E+A++ + E I+D+ ++LV P V++T N++G+++++ A AG
Sbjct: 210 GLFLESCREVAKKYPSITYNEIIVDNCCMQLVAKPEQFDVMVTPNLYGNLVANTAAGIAG 269
Query: 230 SIGIVPSGNYGDEIALFEPIHGSAPDIAGK------GIANPIGAILSAAMMLDYLGLNGK 283
G++P GN G + A+FE G++ GK ANP+ +LS+AMML +L
Sbjct: 270 GTGVMPGGNVGADHAVFE--QGASAGNVGKDKIVLENKANPVALLLSSAMMLRHLQFPSF 327
Query: 284 V--VWEATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
+ A ++ + G T D+GGT+TT EV +I+++
Sbjct: 328 ADRLETAVKKVIAEGKCRTKDLGGTSTTQEVVDAVIAKL 366
>emb|CAA74776.1| (Y14431) NAD-dependent isocitrate dehydrogenase [Nicotiana tabacum]
Length = 371
Score = 150 bits (375), Expect = 2e-35
Identities = 112/340 (32%), Positives = 178/340 (51%), Gaps = 37/340 (10%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRIRF--YEFEGGFEVFKRIGSPISEDDLKEIRKM 61
V +I GDGIGP V + +V++++ + F Y+ G + + + ++ IRK
Sbjct: 44 VTLIPGDGIGPLVTGAVEQVMDAMHAPVYFERYDVHGDMK-------SVPPEVMESIRKN 96
Query: 62 DAILFGATTTPFNVPGYRSLIVTLRKELDLYA------NLRIIPDLSNGKEIVIVRENTE 115
L G TP G SL V LRKELDLYA NL+ +P +IV++RENTE
Sbjct: 97 KVCLKGGLKTPVG-GGVSSLNVQLRKELDLYASLVHCFNLQGLPTRHENVDIVVIRENTE 155
Query: 116 GLYARDGIGFSDRAI-----DFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK- 168
G Y+ G + +++T + RIAK+A A N +T VHKAN++K
Sbjct: 156 GEYS----GLEHEVVPGVVESLKVMTKFCSERIAKYAFEYAYLNNRKVLTAVHKANIMKL 211
Query: 169 GDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIH 227
D F E E+A + G++ E I+D+ ++LV P V++T N++G+++++ A
Sbjct: 212 ADGLFLESCPEVATKYPGIKYNEIIVDNCCMQLVSRPEQFDVMVTPNLYGNLVANTAAGI 271
Query: 228 AGSIGIVPSGNYGDEIALFEPIHGSAPDIAGKGI-----ANPIGAILSAAMMLDYLGLNG 282
AG G++P GN G + A+FE SA ++ + I ANP+ LS+AMML +L
Sbjct: 272 AGGTGVMPGGNVGADHAVFEQ-GASAGNVGNEKILEQKKANPVALFLSSAMMLRHLQFPS 330
Query: 283 KV--VWEATRRYVRYGN-LTPDMGGTATTSEVTKGIISEI 319
+ + +R + G +T D+GG TT E+T +I+ +
Sbjct: 331 FADRLKTSVKRVIAEGKYMTKDLGGNCTTQEITDAVIANL 370
>pir||T13147 isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) beta chain isoform B
- human >gi|4103448|gb|AAD09340.1| (AF023266)
NAD+-specific isocitrate dehydrogenase beta subunit
isoform B [Homo sapiens]
Length = 387
Score = 150 bits (375), Expect = 2e-35
Identities = 106/333 (31%), Positives = 176/333 (52%), Gaps = 16/333 (4%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKM 61
+ V ++ GDG+GPE++ + V + + F E EV E L +++
Sbjct: 49 FPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLS-EVQNMASEEKLEQVLSSMKEN 107
Query: 62 DAILFGATTTPFNVPG-YRSLIVTLRKELDLYANLRIIPDLS------NGKEIVIVRENT 114
+ G TP G S + LR++LDL+AN+ + L N ++VI+RE T
Sbjct: 108 KVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQT 167
Query: 115 EGLYARDGIGFSDRAID-FRIITLEGARRIAKFAINLA-KERNSFITFVHKANVLK-GDR 171
EG Y+ + I+ +I+T ++RIAKFA + A K+ +T VHKAN++K GD
Sbjct: 168 EGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDG 227
Query: 172 FFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGS 230
F + E+AE ++ IID+ ++LV+NP+ V++ N++G+II +LA G
Sbjct: 228 LFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGG 287
Query: 231 IGIVPSGNYGDEIALFEP-IHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVVWE 287
G+VP +Y E A+FE G+ IANP +LSA+ ML +L L + ++ +
Sbjct: 288 AGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIAD 347
Query: 288 ATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
A ++ ++ G + T DMGG +TT++ K +I +
Sbjct: 348 AVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHL 380
>gb|AAD55084.1| (AF176568) isocitrate dehydrogenase gamma subunit [Strongyloides
stercoralis]
Length = 388
Score = 150 bits (375), Expect = 2e-35
Identities = 114/338 (33%), Positives = 177/338 (51%), Gaps = 21/338 (6%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDD--LKEIR 59
+ V ++ GDGIGPE++D ++ + F E + + + SP S+ D + I+
Sbjct: 45 HTVTLLPGDGIGPEMLDIVQKIFTFANVPVDFEECQ----LDSKPTSPESDLDNAIMSIQ 100
Query: 60 KMDAILFGATTTPFNVPGYRSLIVTLRKELDLYAN------LRIIPDLSNGKEIVIVREN 113
+ + G T ++ P ++S V LR+ LDL+AN + IP G IV++REN
Sbjct: 101 RNGVAIKGNIETKYDDPIFKSRNVELRRRLDLFANVLHCVSIPTIPTRHKGINIVMIREN 160
Query: 114 TEGLYARDGIGFSDRAID-FRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK-GD 170
TEG Y+ + ++ +I T E RI++FA + A N IT VHKAN+ K D
Sbjct: 161 TEGEYSGHEHETVNGVVESLKICTREKLERISRFAFDYAIAHNRKKITAVHKANIQKLAD 220
Query: 171 RFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHA 228
F ++V EIAE+E + I+D+ ++LV P ++L N++G+IIS++A
Sbjct: 221 GLFLKVVGEIAEKEYPSITFDSMIVDNASMQLVSRPQQFDIMLMPNLYGNIISNIACGLV 280
Query: 229 GSIGIVPSGNYGDEIALFEP-IHGSAPDIAGKGIANPIGAILSAAMMLDYLGLN--GKVV 285
G G+V N GD+ A+FE + IAG+ IANP I + ML YLGL ++
Sbjct: 281 GGPGLVSGMNIGDKYAVFETGTRNTGSGIAGQDIANPTAFIRAGVDMLHYLGLTEYANLI 340
Query: 286 WEATRRYVRYGNL-TPDMGGTATTSEVTKGIISEIYSF 322
+A + L T D+GGTA TS+V ++ I SF
Sbjct: 341 SDALFDAIVIKRLHTKDIGGTAKTSDVVNAVMRNIESF 378
>emb|CAC01442.1| (AL049712) dJ686C3.1.2 (isocitrate dehydrogenase 3 (NAD+) beta
(isoform B)) [Homo sapiens]
Length = 387
Score = 150 bits (375), Expect = 2e-35
Identities = 106/333 (31%), Positives = 176/333 (52%), Gaps = 16/333 (4%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKM 61
+ V ++ GDG+GPE++ + V + + F E EV E L +++
Sbjct: 49 FPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLS-EVQNMASEEKLEQVLSSMKEN 107
Query: 62 DAILFGATTTPFNVPG-YRSLIVTLRKELDLYANLRIIPDLS------NGKEIVIVRENT 114
+ G TP G S + LR++LDL+AN+ + L N ++VI+RE T
Sbjct: 108 KVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQT 167
Query: 115 EGLYARDGIGFSDRAID-FRIITLEGARRIAKFAINLA-KERNSFITFVHKANVLK-GDR 171
EG Y+ + I+ +I+T ++RIAKFA + A K+ +T VHKAN++K GD
Sbjct: 168 EGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDG 227
Query: 172 FFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGS 230
F + E+AE ++ IID+ ++LV+NP+ V++ N++G+II +LA G
Sbjct: 228 LFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGG 287
Query: 231 IGIVPSGNYGDEIALFEP-IHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVVWE 287
G+VP +Y E A+FE G+ IANP +LSA+ ML +L L + ++ +
Sbjct: 288 AGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIAD 347
Query: 288 ATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
A ++ ++ G + T DMGG +TT++ K +I +
Sbjct: 348 AVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHL 380
>gi|11421208 isocitrate dehydrogenase 3 (NAD+) beta [Homo sapiens]
Length = 385
Score = 150 bits (375), Expect = 2e-35
Identities = 106/333 (31%), Positives = 176/333 (52%), Gaps = 16/333 (4%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKM 61
+ V ++ GDG+GPE++ + V + + F E EV E L +++
Sbjct: 49 FPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLS-EVQNMASEEKLEQVLSSMKEN 107
Query: 62 DAILFGATTTPFNVPG-YRSLIVTLRKELDLYANLRIIPDLS------NGKEIVIVRENT 114
+ G TP G S + LR++LDL+AN+ + L N ++VI+RE T
Sbjct: 108 KVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQT 167
Query: 115 EGLYARDGIGFSDRAID-FRIITLEGARRIAKFAINLA-KERNSFITFVHKANVLK-GDR 171
EG Y+ + I+ +I+T ++RIAKFA + A K+ +T VHKAN++K GD
Sbjct: 168 EGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDG 227
Query: 172 FFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGS 230
F + E+AE ++ IID+ ++LV+NP+ V++ N++G+II +LA G
Sbjct: 228 LFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGG 287
Query: 231 IGIVPSGNYGDEIALFEP-IHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVVWE 287
G+VP +Y E A+FE G+ IANP +LSA+ ML +L L + ++ +
Sbjct: 288 AGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIAD 347
Query: 288 ATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
A ++ ++ G + T DMGG +TT++ K +I +
Sbjct: 348 AVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHL 380
>pir||S48225 3-isopropylmalate dehydrogenase (EC 1.1.1.85) (allele LEa) - yeast
(Candida maltosa) >gi|296350|emb|CAA51442.1| (X72937)
3-isopropylmalate dehydrogenase [Candida maltosa]
Length = 373
Score = 150 bits (374), Expect = 3e-35
Identities = 114/366 (31%), Positives = 183/366 (49%), Gaps = 58/366 (15%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFE-------VFKRIGSPISEDDLK 56
+ ++ GD +G E+V+ AI+V+ ++ + + F+ G P+ +D L+
Sbjct: 8 ITILPGDHVGTEIVNEAIKVLEAIEAATPYQKIHFDFKHHLIGGSAIDATGVPLPDDALE 67
Query: 57 EIRKMDAILFGATTTPFNVPGY---RSLIVTLRKELDLYANLR--------------IIP 99
+ DA+L GA P G ++ +RKEL+LYAN+R + P
Sbjct: 68 SAKNSDAVLLGAVGGPKWGTGALRPEQGLLKIRKELNLYANIRPCNFASDSLLELSPLRP 127
Query: 100 DLSNGKEIVIVRENTEGLYARDGIGFSDR-----------AIDFRIITLEGARRIAKFAI 148
++ G ++IVRE G+Y F DR A D T++ RI + A
Sbjct: 128 EVVKGTNLIIVRELVGGIY------FGDREEQEESEDKQTAWDTEKYTVDEVTRITRIAA 181
Query: 149 NLAKERNSFITF--VHKANVLKGDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNP 204
+A + N + + KANVL R +R V ++ E + V+ +IDS + L++NP
Sbjct: 182 FMALQHNPPLPIWSLDKANVLASSRLWRRTVDKVISEEFPTLSVQHQLIDSAAMILIQNP 241
Query: 205 WD-HGVILTENMFGDIISDLATIHAGSIGIVPSGNYGD------EIALFEPIHGSAPDIA 257
+G+I+T NMFGDIISD A++ GS+G++PS + L+EP HGSAPD+
Sbjct: 242 TKLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDTNTVFGLYEPCHGSAPDLP 301
Query: 258 GKGIANPIGAILSAAMMLDYLGLN----GKVVWEATRRYVRYGNLTPDMGGTATTSEVTK 313
+ NPI ILSAA ML L L+ + + EA ++ + G T D+ GT++T+EV
Sbjct: 302 ANKV-NPIATILSAASML-RLSLDCVKEAEALEEAVKQVLDKGIRTADLRGTSSTTEVGD 359
Query: 314 GIISEI 319
I+ +
Sbjct: 360 AIVEAV 365
>gb|AAG31381.1| (AF197449) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 150 bits (374), Expect = 3e-35
Identities = 105/307 (34%), Positives = 155/307 (50%), Gaps = 31/307 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDR----IRFYEFEGGFEVFKRIGSPISEDDLKE 57
YR+AV+ GDGIGPEV+ A +++N + + I EF G + G + + L
Sbjct: 5 YRIAVLPGDGIGPEVMREAYKILNILKNYFSLPIETREFNVGGAAIDQDGVALPKTTLLG 64
Query: 58 IRKMDAILFGATTTPFNVPGYRSL------IVTLRKELDLYANLR-------------II 98
DAILFG+ ++ LRK +L+ NLR +
Sbjct: 65 CENSDAILFGSXXXXXXXXXXXXXXXXXXXLLPLRKHFNLFGNLRPAQLYSELIHLSPLR 124
Query: 99 PDL-SNGKEIVIVRENTEGLYARDGIGFSDR-----AIDFRIITLEGARRIAKFAINLAK 152
D+ +NG I+ +RE T G+Y G + A D I RIA A LAK
Sbjct: 125 SDIINNGFNILCIRELTGGIYFGKPAGRLKKNNIEYAFDTEIYYNYEINRIAHLAFQLAK 184
Query: 153 ERNSFITFVHKANVLKGDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVIL 211
RN + + K+NVL +REIV ++++ V + ID+ ++++KNP ++L
Sbjct: 185 TRNYKVCSIDKSNVLNSSILWREIVQKVSKNYPDVHLSHLYIDNATMQIIKNPNQFDILL 244
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGD-EIALFEPIHGSAPDIAGKGIANPIGAILS 270
N+FGDIISD + GSIG++PS + + + L+EP GSAPDI GK IANPI ILS
Sbjct: 245 CSNLFGDIISDECAMITGSIGMLPSASLNEKKFGLYEPAGGSAPDIEGKNIANPIAQILS 304
Query: 271 AAMMLDY 277
+M++ Y
Sbjct: 305 VSMLVRY 311
>gb|AAD09339.1| (AF023265) NAD+-specific isocitrate dehydrogenase beta subunit
isoform A [Homo sapiens]
Length = 383
Score = 149 bits (373), Expect = 4e-35
Identities = 106/333 (31%), Positives = 176/333 (52%), Gaps = 16/333 (4%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKM 61
+ V ++ GDG+GPE++ + V + + F E EV E L +++
Sbjct: 49 FPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLS-EVQNMASEEKLEQVLSSMKEN 107
Query: 62 DAILFGATTTPFNVPG-YRSLIVTLRKELDLYANLRIIPDLS------NGKEIVIVRENT 114
+ G TP G S + LR++LDL+AN+ + L N ++VI+RE T
Sbjct: 108 KVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQT 167
Query: 115 EGLYARDGIGFSDRAID-FRIITLEGARRIAKFAINLA-KERNSFITFVHKANVLK-GDR 171
EG Y+ + I+ +I+T ++RIAKFA + A K+ +T VHKAN++K GD
Sbjct: 168 EGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDG 227
Query: 172 FFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGS 230
F + E+AE ++ IID+ ++LV+NP+ V++ N++G+II +LA G
Sbjct: 228 LFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGG 287
Query: 231 IGIVPSGNYGDEIALFEP-IHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVVWE 287
G+VP +Y E A+FE G+ IANP +LSA+ ML +L L + ++ +
Sbjct: 288 AGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIAD 347
Query: 288 ATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
A ++ ++ G + T DMGG AT + T+ +I+ +
Sbjct: 348 AVKKVIKVGKVRTSDMGGYATCHDFTEAVIAAL 380
>emb|CAC01443.1| (AL049712) dJ686C3.1.1 (isocitrate dehydrogenase 3 (NAD+) beta
(isoform A)) [Homo sapiens]
Length = 383
Score = 149 bits (373), Expect = 4e-35
Identities = 106/333 (31%), Positives = 176/333 (52%), Gaps = 16/333 (4%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKEIRKM 61
+ V ++ GDG+GPE++ + V + + F E EV E L +++
Sbjct: 49 FPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLS-EVQNMASEEKLEQVLSSMKEN 107
Query: 62 DAILFGATTTPFNVPG-YRSLIVTLRKELDLYANLRIIPDLS------NGKEIVIVRENT 114
+ G TP G S + LR++LDL+AN+ + L N ++VI+RE T
Sbjct: 108 KVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQT 167
Query: 115 EGLYARDGIGFSDRAID-FRIITLEGARRIAKFAINLA-KERNSFITFVHKANVLK-GDR 171
EG Y+ + I+ +I+T ++RIAKFA + A K+ +T VHKAN++K GD
Sbjct: 168 EGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDG 227
Query: 172 FFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGS 230
F + E+AE ++ IID+ ++LV+NP+ V++ N++G+II +LA G
Sbjct: 228 LFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGG 287
Query: 231 IGIVPSGNYGDEIALFEP-IHGSAPDIAGKGIANPIGAILSAAMMLDYLGL--NGKVVWE 287
G+VP +Y E A+FE G+ IANP +LSA+ ML +L L + ++ +
Sbjct: 288 AGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIAD 347
Query: 288 ATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
A ++ ++ G + T DMGG AT + T+ +I+ +
Sbjct: 348 AVKKVIKVGKVRTSDMGGYATCHDFTEAVIAAL 380
>gi|10954459 3-isopropylmalate dehydrogenase [Buchnera aphidicola]
>gi|3282741|gb|AAD12601.1| (AF041837) 3-isopropylmalate
dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 147 bits (368), Expect = 2e-34
Identities = 105/307 (34%), Positives = 155/307 (50%), Gaps = 31/307 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFY----EFEGGFEVFKRIGSPISEDDLKE 57
Y +AV+ GDGIGPEV+ A +++ + + + EF+ G G + + L
Sbjct: 5 YHIAVLPGDGIGPEVMQEAYKILQVLREHFSLFIKTKEFDIGGIAIDNHGIALPKKTLIG 64
Query: 58 IRKMDAILFGAT------TTPFNVPGYRSLIVTLRKELDLYANLR---------IIPDLS 102
DAIL G+ T P N R+ ++ LRK + + NLR + L
Sbjct: 65 CENSDAILLGSIGGKKWDTLPINERPERASLLPLRKHFNFFCNLRPSNLYKELNFLSPLR 124
Query: 103 N-----GKEIVIVRENTEGLYARDGIGFSDR-----AIDFRIITLEGARRIAKFAINLAK 152
N G +I+ VRE T G+Y G + A D I RIA A LA+
Sbjct: 125 NDIVKHGFDILCVRELTGGIYFGKPRGRVTKKKLMYAFDTEIYYKFEIVRIAHLAFKLAR 184
Query: 153 ERNSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVIL 211
R + + KANVL+ +RE+V E++ E V + ID+ ++++K+P V+L
Sbjct: 185 SRKHKLCSIDKANVLESSILWREVVEEVSKEYPDVILSHLYIDNVCMQIIKDPNQFDVLL 244
Query: 212 TENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKGIANPIGAILS 270
N+FGDIISD + GSIG++PS + ++ L+EP GSAPDI GK IANPI ILS
Sbjct: 245 CSNLFGDIISDECAMITGSIGMLPSASLNEKNFGLYEPAGGSAPDIQGKNIANPIAQILS 304
Query: 271 AAMMLDY 277
+M++ Y
Sbjct: 305 LSMLIRY 311
>sp|Q00412|LEU3_SPIPL 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|152903|gb|AAA26593.1| (M75903)
3-isopropylmalate dehydrogenase [Spirulina platensis]
Length = 355
Score = 142 bits (355), Expect = 5e-33
Identities = 105/317 (33%), Positives = 164/317 (51%), Gaps = 52/317 (16%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRF-YEFE----GGFEVFKRIGSPISEDDLK 56
YR+ ++ GDGIGPE++ A+ V+ +V ++ +EF+ GG + G P+ E+ L+
Sbjct: 5 YRITLLSGDGIGPEIMAVAVDVLKAVGKQLDLNFEFKEALMGGVAI-DATGEPLPEESLQ 63
Query: 57 EIRKMDAILFGATTTPFNVPGYR-----------SLIVTLRKELDLYAN---LRIIP--- 99
R DA+L A + GY+ + ++ LR L L+AN L P
Sbjct: 64 ACRDSDAVLLAA------IGGYKWDNLPRPERPETGLLALRAGLGLFANRARLLFCPHVL 117
Query: 100 -------DLSNGKEIVIVRENTEGLYARDGIGFSDRAIDFRIITLEGAR----------R 142
++ G +I++VRE T G+Y G + EG+ R
Sbjct: 118 DASSLKREVVEGVDIMVVRELTGGIYFGQPKGIFETETGKN----EGSNTMAYGESEIDR 173
Query: 143 IAKFAINLAKERNSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLV 201
I + AK+R + V KANVL + +R+ ++ +A + VE+ +D+ ++LV
Sbjct: 174 IGRVGFETAKKRQGRLCSVDKANVLDVSQLWRDRIMALAADYPEVELSHLYVDNAAMQLV 233
Query: 202 KNPWDHGVILTENMFGDIISDLATIHAGSIGIVPSGNYGDE-IALFEPIHGSAPDIAGKG 260
+ P I+T N+FGDI+SD A + GSIG++PS + G +FEP+HGSAPDIAG+
Sbjct: 234 RWPKQFDTIVTGNLFGDILSDAAAMLTGSIGMLPSASLGASGPGVFEPVHGSAPDIAGQD 293
Query: 261 IANPIGAILSAAMMLDY 277
ANP+ +LSAAMML Y
Sbjct: 294 KANPLAQVLSAAMMLRY 310
>pir||A44851 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Spirulina platensis
Length = 355
Score = 142 bits (355), Expect = 5e-33
Identities = 105/317 (33%), Positives = 164/317 (51%), Gaps = 52/317 (16%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRF-YEFE----GGFEVFKRIGSPISEDDLK 56
YR+ ++ GDGIGPE++ A+ V+ +V ++ +EF+ GG + G P+ E+ L+
Sbjct: 5 YRITLLSGDGIGPEIMAVAVDVLKAVGKQLDLNFEFKEALMGGVAI-DATGEPLPEESLQ 63
Query: 57 EIRKMDAILFGATTTPFNVPGYR-----------SLIVTLRKELDLYAN---LRIIP--- 99
R DA+L A + GY+ + ++ LR L L+AN L P
Sbjct: 64 ACRDSDAVLLAA------IGGYKWDNLPRPERPETGLLALRAGLGLFANRARLLFCPHVL 117
Query: 100 -------DLSNGKEIVIVRENTEGLYARDGIGFSDRAIDFRIITLEGAR----------R 142
++ G +I++VRE T G+Y G + EG+ R
Sbjct: 118 DASSLKREVVEGVDIMVVRELTGGIYFGQPKGIFETETGKN----EGSNTMAYGESEIDR 173
Query: 143 IAKFAINLAKERNSFITFVHKANVLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLV 201
I + AK+R + V KANVL + +R+ ++ +A + VE+ +D+ ++LV
Sbjct: 174 IGRVGFETAKKRQGRLCSVDKANVLDVSQLWRDRIMALAADYPEVELSHLYVDNAAMQLV 233
Query: 202 KNPWDHGVILTENMFGDIISDLATIHAGSIGIVPSGNYGDEIA-LFEPIHGSAPDIAGKG 260
+ P I+T N+FGDI+SD A + GSIG++PS + G +FEP+HGSAPDIAG+
Sbjct: 234 RWPKQFDTIVTGNLFGDILSDAAAMLTGSIGMLPSASLGASPGGVFEPVHGSAPDIAGQD 293
Query: 261 IANPIGAILSAAMMLDY 277
ANP+ +LSAAMML Y
Sbjct: 294 KANPLAQVLSAAMMLRY 310
>pir||T21799 hypothetical protein F35G12.2 - Caenorhabditis elegans
>gi|3876713|emb|CAA86325.1| (Z46242) contains similarity
to Pfam domain: PF00180 (Isocitrate and isopropylmalate
dehydrogenases), Score=269.2, E-value=3.8e-80, N=1~cDNA
EST EMBL:M89207 comes from this gene~cDNA EST yk216a10.5
comes from this gene [Caenorhabditis elegans]
Length = 380
Score = 141 bits (353), Expect = 9e-33
Identities = 106/332 (31%), Positives = 169/332 (49%), Gaps = 38/332 (11%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKE---- 57
+ V V+ GDGIGPE++ R++++V + FEV + +DL E
Sbjct: 52 HNVTVLPGDGIGPEMLHHVERILSAVQAPV-------DFEVVNLTSKEDASEDLAEAITA 104
Query: 58 IRKMDAILFGATTTPFNVPGYRSLIVTLRKELDLYAN------LRIIPDLSNGKEIVIVR 111
I++ L G T F+ P + S + LR++L+LYAN + +P G ++VI+R
Sbjct: 105 IKRNGVALKGNIETKFDNPSFVSRNLELRRQLNLYANVLHCSTIPTVPSRHTGIDMVIIR 164
Query: 112 ENTEGLYARDGIGFSDRAID---------FRIITLEGARRIAKFAINLAKERN-SFITFV 161
ENTEG Y+ G A++ +++T E + +I +FA AK+ +T V
Sbjct: 165 ENTEGEYS----GNEHEAVNAPHPRVVESLKVVTREKSEQITRFAFQFAKKYGRKKVTAV 220
Query: 162 HKANVLK-GDRFFREIVLEIAERE--GVEVREAIIDSFMIKLVKNPWDHGVILTENMFGD 218
HKAN+ K GD F ++ +IA+ E +E I+D+ ++LV P V+L N++G+
Sbjct: 221 HKANIQKLGDGLFLKVATDIAKAEYPDIEFNAMIVDNASMQLVSRPQQFDVMLMPNLYGN 280
Query: 219 IISDLATIHAGSIGIVPSGNYGDEIALFEP-IHGSAPDIAGKGIANPIGAILSAAMMLDY 277
IIS++A G G+V N G++ A+FE + +AGK +ANP I +A ML +
Sbjct: 281 IISNIACGLVGGPGLVSGMNIGEDYAVFETGTRNTGTTLAGKDLANPTAFIRAAVDMLRF 340
Query: 278 LGLNG---KVVWEATRRYVRYGNLTPDMGGTA 306
LGL + R V T D+GGT+
Sbjct: 341 LGLQSHADMISDSLFRTLVDKRIHTADIGGTS 372
>sp|P41564|IDHG_MACFA ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT GAMMA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|631043|pir||S39065 isocitrate dehydrogenase (NAD+)
(EC 1.1.1.41) gamma chain precursor - crab-eating
macaque (fragment) >gi|415979|emb|CAA52224.1| (X74124)
NAD+-isocitrate dehydrogenase, gamma subunit [Macaca
fascicularis]
Length = 355
Score = 139 bits (348), Expect = 3e-32
Identities = 111/337 (32%), Positives = 171/337 (49%), Gaps = 27/337 (8%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKE---- 57
+ V +I GDGIGPE++ V + F E + S E+D++
Sbjct: 17 HTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVH--------VSSNADEEDIRNAIMA 68
Query: 58 IRKMDAILFGATTTPFNVP-GYRSLIVTLRKELDLYANL---RIIPDLSNGK---EIVIV 110
IR+ L G T N+P ++S LR LDLYAN+ + +P + +I+IV
Sbjct: 69 IRRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIV 128
Query: 111 RENTEGLYAR-DGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK 168
RENTEG Y+ + + +IIT + RIA++A LA+E +T VHKAN++K
Sbjct: 129 RENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMK 188
Query: 169 -GDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATI 226
GD F + E+A R + I+D+ ++LV P V++ N++G+I++++
Sbjct: 189 LGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAG 248
Query: 227 HAGSIGIVPSGNYGDEIALFE-PIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLNGKV- 284
G G+V NYG A+FE + IA K IANP +L++ MMLD+L L+
Sbjct: 249 LVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 308
Query: 285 -VWEATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
+ +A + N+ TPD+GG TTSE + II I
Sbjct: 309 SIRKAVLASMDNENMHTPDIGGQGTTSEAIQDIIRHI 345
>gi|4758582 isocitrate dehydrogenase 3 (NAD+) gamma [Homo sapiens]
>gi|1708404|sp|P51553|IDHG_HUMAN ISOCITRATE
DEHYDROGENASE [NAD] SUBUNIT GAMMA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|1167849|emb|CAA93143.1| (Z68907) NAD (H)-specific
isocitrate dehydrogenase gamma subunit precursor [Homo
sapiens] >gi|1302655|gb|AAC51744.1| (U52111)
NAD-isocitrate dehydrogenase [Homo sapiens]
>gi|1673432|emb|CAA92214.1| (Z68129) NAD(H)-specific
isocitrate dehydrogenase gamma-subunit precursor [Homo
sapiens] >gi|4096803|gb|AAD09357.1| (U40272)
NAD+-specific isocitrate dehydrogenase gamma subunit
precursor [Homo sapiens]
Length = 393
Score = 139 bits (347), Expect = 4e-32
Identities = 110/337 (32%), Positives = 171/337 (50%), Gaps = 27/337 (8%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKE---- 57
+ V +I GDGIGPE++ V + F E + S E+D++
Sbjct: 55 HTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVH--------VSSNADEEDIRNAIMA 106
Query: 58 IRKMDAILFGATTTPFNVP-GYRSLIVTLRKELDLYANL---RIIPDLSNGK---EIVIV 110
IR+ L G T N+P ++S LR LDLYAN+ + +P + +I+IV
Sbjct: 107 IRRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIV 166
Query: 111 RENTEGLYAR-DGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK 168
RENTEG Y+ + + +IIT + RIA++A LA+E +T VHKAN++K
Sbjct: 167 RENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMK 226
Query: 169 -GDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATI 226
GD F + E+A R + I+D+ ++LV P V++ N++G+I++++
Sbjct: 227 LGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAG 286
Query: 227 HAGSIGIVPSGNYGDEIALFE-PIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLNGKV- 284
G G+V NYG A+FE + IA K IANP +L++ MMLD+L L+
Sbjct: 287 LVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 346
Query: 285 -VWEATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
+ +A + N+ TPD+GG TTSE + +I I
Sbjct: 347 SIRKAVLASMDNENMHTPDIGGQGTTSEAIQDVIRHI 383
>gi|11419927 isocitrate dehydrogenase 3 (NAD+) gamma [Homo sapiens]
Length = 556
Score = 139 bits (347), Expect = 4e-32
Identities = 110/337 (32%), Positives = 171/337 (50%), Gaps = 27/337 (8%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKE---- 57
+ V +I GDGIGPE++ V + F E + S E+D++
Sbjct: 218 HTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVH--------VSSNADEEDIRNAIMA 269
Query: 58 IRKMDAILFGATTTPFNVP-GYRSLIVTLRKELDLYANL---RIIPDLSNGK---EIVIV 110
IR+ L G T N+P ++S LR LDLYAN+ + +P + +I+IV
Sbjct: 270 IRRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIV 329
Query: 111 RENTEGLYAR-DGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK 168
RENTEG Y+ + + +IIT + RIA++A LA+E +T VHKAN++K
Sbjct: 330 RENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMK 389
Query: 169 -GDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATI 226
GD F + E+A R + I+D+ ++LV P V++ N++G+I++++
Sbjct: 390 LGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAG 449
Query: 227 HAGSIGIVPSGNYGDEIALFE-PIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLNGKV- 284
G G+V NYG A+FE + IA K IANP +L++ MMLD+L L+
Sbjct: 450 LVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 509
Query: 285 -VWEATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
+ +A + N+ TPD+GG TTSE + +I I
Sbjct: 510 SIRKAVLASMDNENMHTPDIGGQGTTSEAIQDVIRHI 546
>dbj|BAA13177.2| (D86855) isocitrate dehydrogenase [Caldococcus noboribetus]
Length = 429
Score = 138 bits (344), Expect = 1e-31
Identities = 125/396 (31%), Positives = 184/396 (45%), Gaps = 84/396 (21%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTD-------RIRFYEFEGGFEVFKRIGSPISEDDLK 56
VA KGDGIGPE+V+SA +V+++ D RI ++E G E K GS + + L+
Sbjct: 35 VAYFKGDGIGPEIVESAKKVLDAAVDKAYGGTRRIVWWEVTAGEEAQKECGSLLPDGTLQ 94
Query: 57 EIRKMDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK--------EIV 108
+ L G TTP G+RSL VTLR LDLY+N+R + + V
Sbjct: 95 AFKLARVNLKGPLTTPVG-GGFRSLNVTLRMVLDLYSNVRPVKWYGQPTPHCHPENIDWV 153
Query: 109 IVRENTEGLYA--------------RD------GIGFS-DRAIDFRIITLEGARRIAKFA 147
I RENTE +YA RD GI + D I + I+ +R + A
Sbjct: 154 IFRENTEDVYAGIEWPFDSPEAQKIRDFLKKEFGIELTPDTGIGIKPISKWRTQRHVRRA 213
Query: 148 INLAKERNSF--ITFVHKANVLKGDRF-------------FREIVL---EIAEREG---- 185
+ A RN + +T +HK N++K FR+ V+ E+ + G
Sbjct: 214 MEWAI-RNGYKHVTIMHKGNIMKYTEGAFRQWAYDLILSEFRDYVVTEEEVNTKYGGKAP 272
Query: 186 ---VEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGSIGIVPSGNYGDE 242
+ V + I D+ + +++ P ++ VI+T N+ GD ISD A G IG+ + GD
Sbjct: 273 EGKIIVNDRIADNMLQQIITRPGEYNVIVTPNLNGDYISDEANALVGGIGMAAGLDMGDG 332
Query: 243 IALFEPIHGSAPDIAGKGIANPIGAILSAAMML-DYLGLNGKVVWEATRRYVRYG----- 296
IA+ EP+HGSAP AGK + NP ILS +L D++G W + V Y
Sbjct: 333 IAVAEPVHGSAPKYAGKNVINPTAEILSGMYLLSDFVG------WPEVKLLVEYAVKQAI 386
Query: 297 -------NLTPDMGGTA--TTSEVTKGIISEIYSFD 323
+L +MGG +T+E T ++ I D
Sbjct: 387 AHKQVTYDLAREMGGVTPISTTEYTDVLVDYIRHAD 422
>pir||T44658 isocitrate dehydrogenase (NADP+) (EC 1.1.1.42) [validated] -
Caldococcus noboribetus
Length = 433
Score = 137 bits (342), Expect = 2e-31
Identities = 125/396 (31%), Positives = 184/396 (45%), Gaps = 84/396 (21%)
Query: 4 VAVIKGDGIGPEVVDSAIRVVNSVTD-------RIRFYEFEGGFEVFKRIGSPISEDDLK 56
VA KGDGIGPE+V+SA +V+++ D RI ++E G E K GS + + L+
Sbjct: 35 VAYFKGDGIGPEIVESAKKVLDAAVDKAYGGTRRIVWWEVTAGEEAQKECGSLLPDGTLQ 94
Query: 57 EIRKMDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRIIPDLSNGK--------EIV 108
+ L G TTP G+RSL VTLR LDLY+N+R + + V
Sbjct: 95 AFKLARVNLKGPLTTPVG-GGFRSLNVTLRMVLDLYSNVRPVKWYGQPTPHCHPENIDWV 153
Query: 109 IVRENTEGLYA--------------RD------GIGFS-DRAIDFRIITLEGARRIAKFA 147
I RENTE +YA RD GI + D I + I+ +R + A
Sbjct: 154 IFRENTEDVYAGIEWPFDSPEAQKIRDFLKKEFGIELTPDTGIGIKPISKWRTQRHVRRA 213
Query: 148 INLAKERNSF--ITFVHKANVLKGDRF-------------FREIVL---EIAEREG---- 185
+ A RN + +T +HK N++K FR+ V+ E+ + G
Sbjct: 214 MEWAI-RNGYKHVTIMHKGNIMKYTEGAFRQWAYDLILSEFRDYVVTEEEVNTKYGGKAP 272
Query: 186 ---VEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIHAGSIGIVPSGNYGDE 242
+ V + I D+ + +++ P ++ VI+T N+ GD ISD A G IG+ + GD
Sbjct: 273 EGKIIVNDRIADNMLQQIITRPGEYNVIVTPNLNGDYISDEANPLVGGIGMAAGLDMGDG 332
Query: 243 IALFEPIHGSAPDIAGKGIANPIGAILSAAMML-DYLGLNGKVVWEATRRYVRYG----- 296
IA+ EP+HGSAP AGK + NP ILS +L D++G W + V Y
Sbjct: 333 IAVAEPVHGSAPKYAGKNVINPTAEILSGMYLLSDFVG------WPEVKLLVEYAVKQAI 386
Query: 297 -------NLTPDMGGTA--TTSEVTKGIISEIYSFD 323
+L +MGG +T+E T ++ I D
Sbjct: 387 AHKQVTYDLAREMGGVTPISTTEYTDVLVDYIRHAD 422
>emb|CAA52225.1| (X74125) NAD+-isocitrate dehydrogenase, gamma subunit [Rattus
norvegicus]
Length = 388
Score = 136 bits (338), Expect = 5e-31
Identities = 109/337 (32%), Positives = 171/337 (50%), Gaps = 27/337 (8%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKE---- 57
+ V +I GDGIGPE++ V + F E + S E+D++
Sbjct: 50 HTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVH--------VSSNADEEDIRNAIMA 101
Query: 58 IRKMDAILFGATTTPFNVP-GYRSLIVTLRKELDLYANL---RIIPDLSNGK---EIVIV 110
IR+ L G T ++P ++S LR LDLYAN+ + +P + +I+IV
Sbjct: 102 IRRNRVALKGNIETNHDLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIV 161
Query: 111 RENTEGLYAR-DGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK 168
RENTEG Y+ + + +IIT + RIA++A LA+E +T VHKAN++K
Sbjct: 162 RENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMK 221
Query: 169 -GDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATI 226
GD F + E+A R + I+D+ ++LV P V++ N++G+I++++
Sbjct: 222 LGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAG 281
Query: 227 HAGSIGIVPSGNYGDEIALFE-PIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLNGKV- 284
G G+V NYG A+FE + IA K IANP +L++ MMLD+L L+
Sbjct: 282 LVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 341
Query: 285 -VWEATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
+ +A + N+ TPD+GG TTS+ + II I
Sbjct: 342 SIRKAVLASMDNENMHTPDIGGQGTTSQAIQDIIRHI 378
>pir||S39064 isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) gamma chain precursor
- rat (fragment)
Length = 388
Score = 136 bits (338), Expect = 5e-31
Identities = 109/337 (32%), Positives = 171/337 (50%), Gaps = 27/337 (8%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKE---- 57
+ V +I GDGIGPE++ V + F E + S E+D++
Sbjct: 50 HTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVH--------VSSNADEEDIRNAIMA 101
Query: 58 IRKMDAILFGATTTPFNVP-GYRSLIVTLRKELDLYANL---RIIPDLSNGK---EIVIV 110
IR+ L G T ++P ++S LR LDLYAN+ + +P + +I+IV
Sbjct: 102 IRRNRVALKGNIETNHDLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIV 161
Query: 111 RENTEGLYAR-DGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK 168
RENTEG Y+ + + +IIT + RIA++A LA+E +T VHKAN++K
Sbjct: 162 RENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMK 221
Query: 169 -GDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATI 226
GD F + E+A R + I+D+ ++LV P V++ N++G+I++++
Sbjct: 222 LGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAG 281
Query: 227 HAGSIGIVPSGNYGDEIALFE-PIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLNGKV- 284
G G+V NYG A+FE + IA K IANP +L++ MMLD+L L+
Sbjct: 282 LVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 341
Query: 285 -VWEATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
+ +A + N+ TPD+GG TTS+ + II I
Sbjct: 342 SIRKAVLASMDNENMHTPDIGGQGTTSQAIQDIIRHI 378
>gi|6680345 isocitrate dehydrogenase 3 (NAD+), gamma [Mus musculus]
>gi|2829481|sp|P70404|IDHG_MOUSE ISOCITRATE
DEHYDROGENASE [NAD] SUBUNIT GAMMA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|1546829|gb|AAC53340.1| (U68564) NAD(H)-specific
isocitrate dehydrogenase gamma subunit precursor [Mus
musculus] >gi|6651053|gb|AAF22149.1|AF133093_4
(AF133093) NAD(H)-specific isocitrate dehydrogenase
gamma subunit [Mus musculus]
Length = 393
Score = 136 bits (338), Expect = 5e-31
Identities = 109/337 (32%), Positives = 170/337 (50%), Gaps = 27/337 (8%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKE---- 57
+ V +I GDGIGPE++ V + F E + S E+D++
Sbjct: 55 HTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVH--------VSSNADEEDIRNAIMA 106
Query: 58 IRKMDAILFGATTTPFNVP-GYRSLIVTLRKELDLYANL---RIIPDLSNGK---EIVIV 110
IR+ L G T N+P ++S LR LDLYAN+ + +P + +I+IV
Sbjct: 107 IRRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIV 166
Query: 111 RENTEGLYAR-DGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK 168
RENTEG Y+ + + +IIT + RIA++A LA+E +T VHKAN++K
Sbjct: 167 RENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMK 226
Query: 169 -GDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATI 226
GD F + E+A + I+D+ ++LV P V++ N++G+I++++
Sbjct: 227 LGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAG 286
Query: 227 HAGSIGIVPSGNYGDEIALFE-PIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLNGKV- 284
G G+V NYG A+FE + IA K IANP +L++ MMLD+L L+
Sbjct: 287 LVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 346
Query: 285 -VWEATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
+ +A + N+ TPD+GG TTS+ + II I
Sbjct: 347 SIRKAVLASMDNENMHTPDIGGQGTTSQAIQDIIRHI 383
>sp|P41565|IDHG_RAT ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT GAMMA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
Length = 393
Score = 136 bits (338), Expect = 5e-31
Identities = 109/337 (32%), Positives = 171/337 (50%), Gaps = 27/337 (8%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLKE---- 57
+ V +I GDGIGPE++ V + F E + S E+D++
Sbjct: 55 HTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVH--------VSSNADEEDIRNAIMA 106
Query: 58 IRKMDAILFGATTTPFNVP-GYRSLIVTLRKELDLYANL---RIIPDLSNGK---EIVIV 110
IR+ L G T ++P ++S LR LDLYAN+ + +P + +I+IV
Sbjct: 107 IRRNRVALKGNIETNHDLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIV 166
Query: 111 RENTEGLYAR-DGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK 168
RENTEG Y+ + + +IIT + RIA++A LA+E +T VHKAN++K
Sbjct: 167 RENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMK 226
Query: 169 -GDRFFREIVLEIAER-EGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATI 226
GD F + E+A R + I+D+ ++LV P V++ N++G+I++++
Sbjct: 227 LGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAG 286
Query: 227 HAGSIGIVPSGNYGDEIALFE-PIHGSAPDIAGKGIANPIGAILSAAMMLDYLGLNGKV- 284
G G+V NYG A+FE + IA K IANP +L++ MMLD+L L+
Sbjct: 287 LVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 346
Query: 285 -VWEATRRYVRYGNL-TPDMGGTATTSEVTKGIISEI 319
+ +A + N+ TPD+GG TTS+ + II I
Sbjct: 347 SIRKAVLASMDNENMHTPDIGGQGTTSQAIQDIIRHI 383
>sp|P87257|LE3B_ASPNG 3-ISOPROPYLMALATE DEHYDROGENASE B (BETA-IPM DEHYDROGENASE B) (IMDH
B) (3-IPM-DH B) >gi|7431320|pir||S72210
3-isopropylmalate dehydrogenase (EC 1.1.1.85) B -
Aspergillus niger >gi|1911743|gb|AAB50828.1| (S83229)
beta-isopropylmalate dehydrogenase=isozyme [Aspergillus
niger, strain A733, Peptide, 371 aa]
>gi|4097257|gb|AAD10279.1| (U51131) beta-isopropylmalate
dehydrogenase [Aspergillus niger]
Length = 371
Score = 134 bits (334), Expect = 1e-30
Identities = 110/362 (30%), Positives = 173/362 (47%), Gaps = 45/362 (12%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSV-TDRIRFY---EFEGGFEVFKRIGSPISEDDLKE 57
Y + V+ GDGIGPEV+ AI+V+ + + ++F+ E GG + G +++
Sbjct: 7 YNILVLPGDGIGPEVMAEAIKVLRTFNSSSMQFHLQEELIGGISIDTH-GHSVTQPVKDA 65
Query: 58 IRKMDAILFGATTTPF------NVPGYRSLIVTLRKELDLYANLR----------IIPDL 101
DA+LF A + G ++ +RK +D+YANLR I D
Sbjct: 66 AVAADAVLFAAVGGSKVDHIRRGLDGPEGGLLQVRKAMDIYANLRPCSVDVPSREIARDF 125
Query: 102 S-------NGKEIVIVRENTEGLYARDGIGFSDRAIDFRIITLEGARRIAKFAINLAKER 154
S G + V+VREN G Y + + A+D + +RIA+ A LA
Sbjct: 126 SPFRQEVIEGVDFVVVRENCGGAYFGKKVEEENYAMDEWGYSTTEIQRIARLAAELALRH 185
Query: 155 NSF--ITFVHKANVLKGDRFFREIVLEIAEREGVEVR--EAIIDSFMIKLVKNPWD-HGV 209
+ + + KANVL R +R +V E +V+ + DS + + +P +GV
Sbjct: 186 DPPWPVISLDKANVLASSRLWRRVVENTISVEYPQVKLVHQLADSASLIMATDPRVLNGV 245
Query: 210 ILTENMFGDIISDLATIHAGSIGIVPS---------GNYGDEIALFEPIHGSAPDIAGKG 260
IL +N FGD++SD A G++G++PS G L+EP HGSAP IAGK
Sbjct: 246 ILADNTFGDMLSDQAGSLIGTLGVLPSASLDGLPHPGKQEKVRGLYEPTHGSAPTIAGKN 305
Query: 261 IANPIGAILSAAMMLDY---LGLNGKVVWEATRRYVRYGNLTPDMGGTATTSEVTKGIIS 317
IANP IL ++M Y + + + +A R + G TPD+GG ++T E +++
Sbjct: 306 IANPTAMILCVSLMFRYSFNMENEARQIEDAVRAVLDRGLRTPDLGGNSSTQEFGDAVVA 365
Query: 318 EI 319
+
Sbjct: 366 AL 367
>gb|AAF56494.1| (AE003753) CG5028 gene product [Drosophila melanogaster]
Length = 393
Score = 132 bits (330), Expect = 4e-30
Identities = 102/333 (30%), Positives = 166/333 (49%), Gaps = 25/333 (7%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTDRIRFYEFEGGFEVFKRIGSPISEDDLK----E 57
+ V ++ G GIGPE++ V + R+ FEV S DDL
Sbjct: 58 HAVTMLPGGGIGPELM-------GYVREIFRYCGAPIDFEVIDIDPSTEGNDDLDYAITS 110
Query: 58 IRKMDAILFGATTTPFNVPGYRSLIVTLRKELDLYANLRI------IPDLSNGKEIVIVR 111
I++ L G T S V +R ELDLY N+ IP + ++V++R
Sbjct: 111 IKRNGVALKGNIETKSQSLTEVSRNVAIRNELDLYVNVVHCKSYPGIPARHHDIDVVLIR 170
Query: 112 ENTEGLYAR-DGIGFSDRAIDFRIITLEGARRIAKFAINLAKERN-SFITFVHKANVLK- 168
+NT+G YA + +++T+E A R+A++A A++ N +T +HKAN++K
Sbjct: 171 QNTDGEYAMLEHESVPGIVESMKVVTVENAERVARYAFEFARQNNRKKVTTIHKANIMKL 230
Query: 169 GDRFFREIVLEI-AEREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDIISDLATIH 227
D F E+ + + +E IID+ ++ V NP V+ N++G I+S++
Sbjct: 231 SDGLFLEVANRVHKDYPELEHNNMIIDNTCMQSVSNPHQFDVMNMTNLYGTIVSNVLCGL 290
Query: 228 AGSIGIVPSGNYGDEIALFEP-IHGSAPDIAGKGIANPIGAILSAAMMLDYLG--LNGKV 284
G G++ NYGD A+FEP + IAGK IANP+ I ++ ML++LG + V
Sbjct: 291 MGGAGLISGRNYGDHYAIFEPGTRNTGTAIAGKNIANPVAMISASIDMLNHLGHKEHANV 350
Query: 285 VWEAT-RRYVRYGNLTPDMGGTATTSEVTKGII 316
+ EA + V TPD+GGT ++++V + I+
Sbjct: 351 IQEAVYQTIVNDAIRTPDIGGTNSSTDVVENIL 383
>sp|P42958|YCSA_BACSU HYPOTHETICAL 33.1 KD PROTEIN IN MTLD-SIPU INTERGENIC REGION
>gi|7431322|pir||A69765 tartrate dehydrogenase (EC
1.1.1.93) - Bacillus subtilis
>gi|2632701|emb|CAB12208.1| (Z99106) similar to
3-isopropylmalate dehydrogenase [Bacillus subtilis]
Length = 301
Score = 132 bits (329), Expect = 6e-30
Identities = 89/288 (30%), Positives = 148/288 (50%), Gaps = 31/288 (10%)
Query: 2 YRVAVIKGDGIGPEVVDSAIRVVNSVTD-----RIRFYEFEGGFEVFKRIGSPISEDDLK 56
+ +A I GDG+G EVV +A +V+++ + F F + + G + ED +
Sbjct: 6 FEIAAIPGDGVGKEVVAAAEKVLHTAAEVHGGLSFSFTAFPWSCDYYLEHGKMMPEDGIH 65
Query: 57 EIRKMDAILFGATTTPFNVPGYRSL---IVTLRKELDLYANLRIIPDLS---------NG 104
+ + +A+ GA P VP + SL ++ +R+EL+L N+R ++ N
Sbjct: 66 TLTQFEAVFLGAVGNPKLVPDHISLWGLLLKIRRELELSINMRPAKQMAGITSPLLHPND 125
Query: 105 KEIVIVRENTEGLYARDG----IGFSDRAIDFRIITLEGARRIAKFAINLAKERNSFITF 160
+ V++REN+EG Y+ G G + AI + T + R+ +FA LAK+R + +T
Sbjct: 126 FDFVVIRENSEGEYSEVGGRIHRGDDEIAIQNAVFTRKATERVMRFAFELAKKRRTLVTS 185
Query: 161 VHKAN-VLKGDRFFREIVLEIA-EREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGD 218
K+N + F+ E+ + A + G+E ID+ V P VI+ +FGD
Sbjct: 186 ATKSNGIYHAMPFWDEVFQQTAADYSGIETSSQHIDALAAFFVTRPETFDVIVASKLFGD 245
Query: 219 IISDLATIHAGSIGIV-----PSGNYGDEIALFEPIHGSAPDIAGKGI 261
I++D+++ SIGI PSG Y ++FEP+HGSAPDIAG+ +
Sbjct: 246 ILTDISSSLMESIGIAPPDINPSGKYP---SMFEPVHGSAPDIAGQAL 290
>gb|AAC83168.1| (AF090323) NAD(+)-isocitrate dehydrogenase subunit 1 IDH1-C
precursor [Bos taurus]
Length = 128
Score = 77.3 bits (187), Expect = 3e-13
Identities = 42/126 (33%), Positives = 73/126 (57%), Gaps = 4/126 (3%)
Query: 201 VKNPWDHGVILTENMFGDIISDLATIHAGSIGIVPSGNYGDEIALFEP-IHGSAPDIAGK 259
V+NP+ V++ N++G+II +LA G G+VP +Y E A+FE G+
Sbjct: 1 VQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGR 60
Query: 260 GIANPIGAILSAAMMLDYLGL--NGKVVWEATRRYVRYGNL-TPDMGGTATTSEVTKGII 316
IANP +LSA+ ML +L L + ++ EA ++ ++ G + T DMGG +TT++ + +I
Sbjct: 61 NIANPTAMLLSASNMLRHLNLEHHSNMIAEAVKKVIKVGKVRTRDMGGYSTTTDFIRSVI 120
Query: 317 SEIYSF 322
++ +
Sbjct: 121 GHLHPY 126
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.322 0.143 0.415
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 127716415
Number of Sequences: 2977
Number of extensions: 5418386
Number of successful extensions: 13228
Number of sequences better than 1.0e-10: 266
Number of HSP's better than 0.0 without gapping: 101
Number of HSP's successfully gapped in prelim test: 165
Number of HSP's that attempted gapping in prelim test: 11823
Number of HSP's gapped (non-prelim): 273
length of query: 346
length of database: 189,106,746
effective HSP length: 54
effective length of query: 292
effective length of database: 156,788,448
effective search space: 45782226816
effective search space used: 45782226816
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 165 (68.7 bits)