BLASTP 2.0.10 [Aug-26-1999]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= PAB0399 (hypA) DE:hydrogenase expression/formation protein
(hypA)
         (139 letters)

Database: ./suso.pep; /banques/blast2/nr.pep
           598,487 sequences; 189,106,746 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||H75177 hydrogenase expression/formation protein (hypa) PAB0...   285  1e-76
pir||E71036 hypothetical protein PH1581 - Pyrococcus horikoshii ...   249  1e-65
pir||F69204 hydrogenase expression/formation protein HypA - Meth...    90  1e-17
pir||B70388 hydrogenase accessory protein HypA - Aquifex aeolicu...    71  8e-12

>pir||H75177 hydrogenase expression/formation protein (hypa) PAB0399 -
           Pyrococcus abyssi (strain Orsay)
           >gi|5458013|emb|CAB49503.1| (AJ248284) hydrogenase
           expression/formation protein (hypA) [Pyrococcus abyssi]
           Length = 139
           
 Score =  285 bits (722), Expect = 1e-76
 Identities = 139/139 (100%), Positives = 139/139 (100%)

Query: 1   MHEWALADAIVRTALDYAQKENASKILAIKVVLGELQDVNAEVVEFAMKELLRGTIGEGA 60
           MHEWALADAIVRTALDYAQKENASKILAIKVVLGELQDVNAEVVEFAMKELLRGTIGEGA
Sbjct: 1   MHEWALADAIVRTALDYAQKENASKILAIKVVLGELQDVNAEVVEFAMKELLRGTIGEGA 60

Query: 61  EIIFEEEEAVFRCRNCGHEWKLKEVKDKLDERIREDIHFIPEVVHAFISCPNCGSHDFEV 120
           EIIFEEEEAVFRCRNCGHEWKLKEVKDKLDERIREDIHFIPEVVHAFISCPNCGSHDFEV
Sbjct: 61  EIIFEEEEAVFRCRNCGHEWKLKEVKDKLDERIREDIHFIPEVVHAFISCPNCGSHDFEV 120

Query: 121 VKGRGVYIAGIKIEKEEGE 139
           VKGRGVYIAGIKIEKEEGE
Sbjct: 121 VKGRGVYIAGIKIEKEEGE 139


>pir||E71036 hypothetical protein PH1581 - Pyrococcus horikoshii
           >gi|3258010|dbj|BAA30693.1| (AP000006) 139aa long
           hypothetical protein [Pyrococcus horikoshii]
           Length = 139
           
 Score =  249 bits (628), Expect = 1e-65
 Identities = 115/136 (84%), Positives = 130/136 (95%)

Query: 1   MHEWALADAIVRTALDYAQKENASKILAIKVVLGELQDVNAEVVEFAMKELLRGTIGEGA 60
           MHEWALADAIVRT L++AQKENASK+LA+KVVLGELQDV+A++VEFAM EL +GTI EGA
Sbjct: 1   MHEWALADAIVRTVLEHAQKENASKVLAVKVVLGELQDVDAKIVEFAMNELFKGTIAEGA 60

Query: 61  EIIFEEEEAVFRCRNCGHEWKLKEVKDKLDERIREDIHFIPEVVHAFISCPNCGSHDFEV 120
           EIIFEEEEAVF+CRNCG+EW+L EV++KLDER+REDIHFIPEVVHAF+SCP CGSHDFEV
Sbjct: 61  EIIFEEEEAVFKCRNCGYEWRLSEVREKLDERMREDIHFIPEVVHAFLSCPRCGSHDFEV 120

Query: 121 VKGRGVYIAGIKIEKE 136
           VKGRGVYIAGIKIEKE
Sbjct: 121 VKGRGVYIAGIKIEKE 136


>pir||F69204 hydrogenase expression/formation protein HypA - Methanobacterium
           thermoautotrophicum (strain Delta H)
           >gi|2621873|gb|AAB85285.1| (AE000856) hydrogenase
           expression/formation protein HypA [Methanobacterium
           thermoautotrophicum]
           Length = 125
           
 Score = 89.7 bits (219), Expect = 1e-17
 Identities = 54/137 (39%), Positives = 76/137 (55%), Gaps = 15/137 (10%)

Query: 1   MHEWALADAIVRTALDYAQKENASKILAIKVVLGELQDVNAEVVEFAMKELLRGTIGEGA 60
           +HE ++ADAIVRT +D A+K +A ++L + V +G+L  +N E +EF +  L  GTI EGA
Sbjct: 4   VHELSMADAIVRTVIDAAEKNDAVEVLEVTVEIGQLTLLNPEQIEFMLDVLSEGTILEGA 63

Query: 61  EIIFEEEEAVFRCRNCGHEWKLKEVKDKLDERIREDIHFIPEVVHAFISCPNCGSHDFEV 120
               E       C  CG+E  ++   D+LD       HF P      I CP CG H+F V
Sbjct: 64  RFNLEVVPVEIEC-ECGYEGVVE--ADELD-------HFAP-----VIKCPACGGHEFHV 108

Query: 121 VKGRGVYIAGIKIEKEE 137
             GR   +  IKIEK++
Sbjct: 109 RAGRECNVKNIKIEKKD 125


>pir||B70388 hydrogenase accessory protein HypA - Aquifex aeolicus
           >gi|2983520|gb|AAC07096.1| (AE000719) hydrogenase
           accessory protein HypA [Aquifex aeolicus]
           Length = 115
           
 Score = 70.6 bits (170), Expect = 8e-12
 Identities = 34/134 (25%), Positives = 67/134 (49%), Gaps = 21/134 (15%)

Query: 1   MHEWALADAIVRTALDYAQKENASKILAIKVVLGELQDVNAEVVEFAMKELLRGTIGEGA 60
           MHE+++  +++    DYA++ NA  +  + V +G L  V   ++E A      GT+ E A
Sbjct: 1   MHEFSIVQSLLTLIEDYARENNAKSVTKVVVSIGVLSGVEPHLLEMAFNTFKEGTVAEKA 60

Query: 61  EIIFEEEEAVFRCRNCGHEWKLKEVKDKLDERIREDIHFIPEVVHAFISCPNCGSHDFEV 120
           E+I E E+   +C +CG E + +E+                      + CP CGS + ++
Sbjct: 61  ELIMEIEKLKIKCMDCGKESEKEELN---------------------MLCPGCGSLNTQI 99

Query: 121 VKGRGVYIAGIKIE 134
           + G+ +++  +++E
Sbjct: 100 ISGQDMFLKSLELE 113


  Database: ./suso.pep
    Posted date:  Jul 6, 2001  5:57 PM
  Number of letters in database: 840,471
  Number of sequences in database:  2977
  
  Database: /banques/blast2/nr.pep
    Posted date:  Dec 14, 2000 12:46 PM
  Number of letters in database: 188,266,275
  Number of sequences in database:  595,510
  
Lambda     K      H
   0.320    0.139    0.407 

Gapped
Lambda     K      H
   0.270   0.0470    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49522769
Number of Sequences: 2977
Number of extensions: 1885082
Number of successful extensions: 5519
Number of sequences better than 1.0e-10: 4
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 5513
Number of HSP's gapped (non-prelim): 4
length of query: 139
length of database: 189,106,746
effective HSP length: 52
effective length of query: 87
effective length of database: 157,985,422
effective search space: 13744731714
effective search space used: 13744731714
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 161 (67.1 bits)