BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0403 (hypE-like) DE:hydrogenase expression/formation related
protein
(326 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||F75178 hydrogenase expression/formation related protein PAB... 645 0.0
pir||E71035 probable hydrogenase expression/formation protein - ... 557 e-158
pir||F72508 probable hydrogenase expression/formation protein AP... 189 5e-47
emb|CAC12402.1| (AL445067) hydrogenase expression/formation prot... 163 2e-39
gb|AAK42820.1| Hydrogenase expression/formation factor related p... 151 9e-36
gb|AAK40418.1| Hydrogenase expression/formation protein (hypE) [... 143 2e-33
pir||S75435 hypothetical protein c05018 - Sulfolobus solfataricu... 143 2e-33
sp|Q58089|Y676_METJA PUTATIVE HYDROGENASE EXPRESSION/FORMATION P... 143 3e-33
pir||B69125 hydrogenase expression/formation protein HypE - Meth... 126 4e-28
gb|AAG18782.1| (AE004983) hydrogenase expression/formation; Hef ... 114 1e-24
pir||A81411 hydrogenase isoenzymes formation protein Cj0626 [imp... 113 2e-24
gi|11498961 hydrogenase expression/formation protein (hypE) [Arc... 107 2e-22
sp|P40595|HYPE_AZOVI HYDROGENASE EXPRESSION/FORMATION PROTEIN HY... 96 7e-19
pir||E75040 hydrogenase expression/formation protein (hype) PAB0... 95 1e-18
gb|AAB64436.1| (AF006594) HupE [Anabaena PCC7120] 93 5e-18
emb|CAA51035.1| (X72294) ORF9 [Azotobacter vinelandii] 92 6e-18
pir||S35767 hypothetical protein 9 - Azotobacter vinelandii 92 6e-18
pir||A70388 hydrogenase expression/formation protein - Aquifex a... 92 6e-18
sp|P42034|HUPE_AZOCH HYDROGENASE EXPRESSION/FORMATION PROTEIN HU... 92 8e-18
pir||D71121 probable hydrogenase expression/formation protein - ... 92 1e-17
sp|P40599|HYPE_RHILV HYDROGENASE EXPRESSION/FORMATION PROTEIN HY... 90 4e-17
sp|P24193|HYPE_ECOLI HYDROGENASE ISOENZYMES FORMATION PROTEIN HY... 83 5e-15
gb|AAA69240.1| (U29579) CG Site no. 33119; ORF_o322 [Escherichia... 83 5e-15
gb|AAG20935.1| (AE005160) hydrogenase expression/formation prote... 80 3e-14
pir||S35230 hypE protein - Bradyrhizobium japonicum >gi|39536|em... 77 2e-13
sp|P31906|HYPE_BRAJA HYDROGENASE EXPRESSION/FORMATION PROTEIN HYPE 77 2e-13
sp|P31905|HYPE_ALCEU HYDROGENASE EXPRESSION/FORMATION PROTEIN HY... 77 3e-13
pir||S21906 hypE protein - Rhodobacter capsulatus >gi|46056|emb|... 77 3e-13
sp|P26412|HYPE_RHOCA HYDROGENASE EXPRESSION/FORMATION PROTEIN HY... 77 3e-13
pir||S77375 hydrogenase expression/formation protein hypE - Syne... 76 7e-13
pir||G64525 hydrogenase expression/formation protein - Helicobac... 75 2e-12
gb|AAC26158.1| (AF035751) HypE [Synechococcus PCC7002] 74 2e-12
pir||F71981 hydrogenase expression/formation protein - Helicobac... 74 2e-12
sp|O27447|THIL_METTH PROBABLE THIAMINE-MONOPHOSPHATE KINASE (THI... 74 3e-12
pir||D69116 phosphoribosylformylglycinamidine synthase II relate... 69 9e-11
>pir||F75178 hydrogenase expression/formation related protein PAB0403 -
Pyrococcus abyssi (strain Orsay)
>gi|5458019|emb|CAB49509.1| (AJ248284) hydrogenase
expression/formation related protein [Pyrococcus abyssi]
Length = 326
Score = 645 bits (1646), Expect = 0.0
Identities = 326/326 (100%), Positives = 326/326 (100%)
Query: 1 MEMLAGKIPPEVLIKIIDGIKPGERVIVGPGVGIDATAIDFGDYVLVASTDPITGAEERI 60
MEMLAGKIPPEVLIKIIDGIKPGERVIVGPGVGIDATAIDFGDYVLVASTDPITGAEERI
Sbjct: 1 MEMLAGKIPPEVLIKIIDGIKPGERVIVGPGVGIDATAIDFGDYVLVASTDPITGAEERI 60
Query: 61 GFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGHT 120
GFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGHT
Sbjct: 61 GFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGHT 120
Query: 121 EVTPGLKRPIVVGTMLGEVDRDNLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEELRGV 180
EVTPGLKRPIVVGTMLGEVDRDNLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEELRGV
Sbjct: 121 EVTPGLKRPIVVGTMLGEVDRDNLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEELRGV 180
Query: 181 FGRDFVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDLGFRIFAE 240
FGRDFVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDLGFRIFAE
Sbjct: 181 FGRDFVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDLGFRIFAE 240
Query: 241 KIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLSYGISASIIGEFLAE 300
KIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLSYGISASIIGEFLAE
Sbjct: 241 KIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLSYGISASIIGEFLAE 300
Query: 301 KKRVIIENGIERPLKRPESDELWKVV 326
KKRVIIENGIERPLKRPESDELWKVV
Sbjct: 301 KKRVIIENGIERPLKRPESDELWKVV 326
>pir||E71035 probable hydrogenase expression/formation protein - Pyrococcus
horikoshii >gi|3258002|dbj|BAA30685.1| (AP000006) 326aa
long hypothetical hydrogenase expression/formation
protein [Pyrococcus horikoshii]
Length = 326
Score = 557 bits (1420), Expect = e-158
Identities = 270/326 (82%), Positives = 305/326 (92%)
Query: 1 MEMLAGKIPPEVLIKIIDGIKPGERVIVGPGVGIDATAIDFGDYVLVASTDPITGAEERI 60
M+ML GKIP E+L KI++ +KPG++VIVGP VGIDATAIDFGDYVLVASTDPITG EE+I
Sbjct: 1 MKMLVGKIPSELLAKILEKLKPGDKVIVGPKVGIDATAIDFGDYVLVASTDPITGTEEKI 60
Query: 61 GFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGHT 120
GFY+V++NANDVATFGA+PKWFLVTIL+PEGS D+ IM E+REV+E MGI+IVGGHT
Sbjct: 61 GFYAVNINANDVATFGARPKWFLVTILLPEGSEFNDVITIMDEVREVAEGMGISIVGGHT 120
Query: 121 EVTPGLKRPIVVGTMLGEVDRDNLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEELRGV 180
EVTPGLK+PIVVGTMLGEV+++NLVVP+P+PGDAII+TKGAGIEGTSIIAHEREEEL+GV
Sbjct: 121 EVTPGLKKPIVVGTMLGEVEKENLVVPNPRPGDAIILTKGAGIEGTSIIAHEREEELKGV 180
Query: 181 FGRDFVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDLGFRIFAE 240
FG +FVERAKAFI EISIVREA+++REFATAMHDPTEGGIANG+HEMADIN LGFRIFA
Sbjct: 181 FGSNFVERAKAFINEISIVREALLSREFATAMHDPTEGGIANGLHEMADINGLGFRIFAN 240
Query: 241 KIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLSYGISASIIGEFLAE 300
K+IIR+ETRKIC FYDL+PLALISSGALLIS PRDHAKFL+ERLLS+GI ASIIGEFLAE
Sbjct: 241 KVIIREETRKICHFYDLDPLALISSGALLISTPRDHAKFLIERLLSHGIQASIIGEFLAE 300
Query: 301 KKRVIIENGIERPLKRPESDELWKVV 326
KKRVIIENG ERPLKRPESDELWKVV
Sbjct: 301 KKRVIIENGAERPLKRPESDELWKVV 326
>pir||F72508 probable hydrogenase expression/formation protein APE2044 -
Aeropyrum pernix (strain K1) >gi|5105742|dbj|BAA81054.1|
(AP000063) 308aa long hypothetical hydrogenase
expression/formation protein [Aeropyrum pernix]
Length = 308
Score = 189 bits (474), Expect = 5e-47
Identities = 116/303 (38%), Positives = 168/303 (55%), Gaps = 17/303 (5%)
Query: 32 VGIDATAIDFGDYVLVASTDPITGAEERIGFYSVHVNANDVATFGAKPKWFLVTILMPEG 91
+G DA + G LV DPIT A G+ +VHV AND+A GA+P+W ++ +LMP G
Sbjct: 1 MGEDAAIVSLGPIDLVMHLDPITEAGALAGWLAVHVAANDIAVTGARPRWEMLAVLMPPG 60
Query: 92 SSEEDIANIMAEIREVSEAMGIAIVGGHTEVTPGLKRPIVVGTMLGEVDRDNLVVPD--P 149
SSE+ +A+++ + +E +G+ IVGGHTE PG+ RPI+V G + P
Sbjct: 61 SSEDSLASLLGDAVRAAEEIGVEIVGGHTEAAPGVPRPIIVAAAAG-ITCGGCTTPTSAA 119
Query: 150 KPGDAIIMTKGAGIEGTSIIAHEREEEL--RGVFGRDFVERAKAFIEEISIVREAMIARE 207
+PGD ++ K A IEGT+IIA + + L RGV G D + A+ +SIV EA+ E
Sbjct: 120 RPGDLVLQVKPAAIEGTAIIATDFADMLRSRGV-GEDAIAGARMLASRVSIVEEAIELAE 178
Query: 208 --FATAMHDPTEGGIANGIHEMADINDLGFRIFAEKIIIRDETRKICDFYDLNPLALISS 265
AMHDPTEGG+ G+ EMA + + +KII+ + TR + ++PL LISS
Sbjct: 179 AGVVRAMHDPTEGGVLGGLVEMALASRTVIEVEEDKIIVGEATRIVASALGIDPLRLISS 238
Query: 266 GALLISVPRD---HAKFLVERLLSYGISASIIGEFLA---EKKRVIIENGIERPLKRPES 319
G LL +VP D A++++E L G+ S IG A E + G + + P
Sbjct: 239 GTLLATVPPDKAGEARYVLEEL---GVEYSFIGRVAARSGEPALRLSSRGRVKTFREPPM 295
Query: 320 DEL 322
DE+
Sbjct: 296 DEI 298
>emb|CAC12402.1| (AL445067) hydrogenase expression/formation protein (Anabaena hupE)
related protein [Thermoplasma acidophilum]
Length = 334
Score = 163 bits (409), Expect = 2e-39
Identities = 95/313 (30%), Positives = 161/313 (51%), Gaps = 14/313 (4%)
Query: 27 IVGPGVGIDATAIDFGDYVLVAST------DPITGAEERIGFYSVHVNANDVATFGAKPK 80
+V P G+D I GD +++ T DP+ G E+ +++ H+ A+D+ T GA P
Sbjct: 14 LVPPANGVDVGIIKIGDGRVMSVTTDPFYFDPVFGYEDS-AWFAYHILASDLLTSGANPD 72
Query: 81 WFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGHTEVTPGLKRPIVVG-TMLGEV 139
+ V + +P ++E+I+ + E + G +I+ GHT G P++ G T +G +
Sbjct: 73 YMTVDLNLPLSMTDEEISRMWNAFTEEAARFGTSIITGHTARYAGTSFPMIGGITAMGFI 132
Query: 140 DRDNLVVPD-PKPGDAIIMTKGAGIEGTSIIAHEREEELRGVFGRDFVERAKAFIEEISI 198
D V +PGD +++TK IE +++ ++ G + +++
Sbjct: 133 GEDEYVTSAMARPGDKVVLTKTPAIEAAALLTRLFPGTIKAKLGDELFSYGVDLFRKLTT 192
Query: 199 VREAMIAREFA-----TAMHDPTEGGIANGIHEMADINDLGFRIFAEKIIIRDETRKICD 253
V E+++ + F TAMHD TEGG+ + E+AD + G RI + I + D +++C
Sbjct: 193 VHESLVCKRFGLRTRVTAMHDVTEGGLLGALFEIADSSGNGIRIHEDSIKVDDYVKRVCS 252
Query: 254 FYDLNPLALISSGALLISVPRDHAKFLVERLLSYGISASIIGEFLAEKKRVIIENGIERP 313
+ ++PL IS GALLI+V DHA+ LV L + GI A++IGE A+ II +R
Sbjct: 253 IFSIDPLMAISEGALLITVKPDHAEDLVSSLKANGIDANVIGEMTADPSERIIVGREKRT 312
Query: 314 LKRPESDELWKVV 326
+ P+ D W V
Sbjct: 313 IGPPQKDPFWTAV 325
>gb|AAK42820.1| Hydrogenase expression/formation factor related protein [Sulfolobus
solfataricus]
Length = 341
Score = 151 bits (378), Expect = 9e-36
Identities = 104/335 (31%), Positives = 170/335 (50%), Gaps = 16/335 (4%)
Query: 3 MLAGKIPPEVLIKIIDGIKPGER---VIVGPGVGIDATAIDFGD-YVLVASTDPITGAEE 58
M GKI +V K+I GER V+VGP G+D AID D VLV TDP+ +
Sbjct: 1 MHLGKINEDVFNKVIYP-HLGERRKEVVVGPQHGVDTGAIDLQDGRVLVVKTDPVFIVPQ 59
Query: 59 ----RIGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIA 114
+ +++VH+ A+DV T G P++ ++ + +P +E+ + I E + +G+
Sbjct: 60 FGFKKAAWFAVHILASDVMTSGIPPEYAVIDLNLPPRIKDEEFEEMWIGIHEALKEIGVM 119
Query: 115 IVGGHTEVTPGLKRPIVVG-TMLGEVDRDNLVVPDP-KPGDAIIMTKGAGIEGTSIIAHE 172
+VGGHT V G P+V G TM G +++ L +P K GD++IMTKG IE T ++ +
Sbjct: 120 VVGGHTGVYEGTDYPMVGGFTMFGIGEKERLGMPSKVKVGDSVIMTKGPAIEATGLLTNL 179
Query: 173 REEELRGVFGRDFVERAKAFIEEISIVREAMIAREFAT-AMHDPTEGGIANGIHEMADIN 231
E + + A ++S ++ +IA MHD TEGG+ + E+A +
Sbjct: 180 YPEYFKQRLSNEIFNEAYNMYWKMSCWKDGLIASRIGIHMMHDATEGGVFGALTEVARAS 239
Query: 232 DLGFRIFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLSYGISA 291
G RI+ E+ I R++ +++P IS G ++I D + + L+ GI A
Sbjct: 240 SKGMRIYEERFFINRAVREVTKLVNIDPWISISEGTMIIVT--DKGEEVRNTLIREGIEA 297
Query: 292 SIIGEFLAEKKRV--IIENGIERPLKRPESDELWK 324
IIGE + + V + ++G ++ P D W+
Sbjct: 298 EIIGEIVDQSGEVTMVKKDGSRVKIEHPLEDPFWR 332
>gb|AAK40418.1| Hydrogenase expression/formation protein (hypE) [Sulfolobus
solfataricus]
Length = 343
Score = 143 bits (358), Expect = 2e-33
Identities = 103/296 (34%), Positives = 164/296 (54%), Gaps = 23/296 (7%)
Query: 6 GKIPPEVLIKIIDGIKPGERVIVGPGVGIDATAIDFGDYVLVASTDPITGAEERIGFYSV 65
GK+P + K I + G+ + P +G DA ++ D L+ +DPIT A+ GF S+
Sbjct: 29 GKVP---INKFIYNLPIGD-CLTCPSIGEDAAVLEAKDKYLIVHSDPITEAKSDSGFLSI 84
Query: 66 HVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEA---MGIAIVGGHTEV 122
V ND+ GA+ +W IL+ DI+++ A I +SEA +G +VGGHTEV
Sbjct: 85 AVACNDINMKGAQCRWVNSIILL------SDISHLNATISAISEACNLIGCKVVGGHTEV 138
Query: 123 TPGLKRPIVVGTMLGEVDRDNLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEEL--RGV 180
+ +K+ I++ T +G V L + K G +++ GIEGT I+A + ++ L +G+
Sbjct: 139 SDKVKQDIIITTAIG-VSNKFLSYKNVKEGMKVVLVGSPGIEGTWILAKDYDQLLLEKGI 197
Query: 181 FGRDFVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDLGFRIFAE 240
R+ +E+AK F ++I + +A+ ++ AMHD TEGGI + E+A + I +
Sbjct: 198 -SREIIEKAKEFKKDIVVQPKALSILDYVVAMHDATEGGIMQAMLEVAKASGYTVSINLD 256
Query: 241 KIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLSYGISASIIGE 296
KI++R ET+ I +++PL LISSG+ I+V K L ER+ A IIGE
Sbjct: 257 KIVLRRETKIITKALNIDPLRLISSGS-FIAVSNTPEKIL-ERI----SEAYIIGE 306
>pir||S75435 hypothetical protein c05018 - Sulfolobus solfataricus
>gi|1707841|emb|CAA69519.1| (Y08257) orf c05018
[Sulfolobus solfataricus]
Length = 343
Score = 143 bits (358), Expect = 2e-33
Identities = 103/296 (34%), Positives = 164/296 (54%), Gaps = 23/296 (7%)
Query: 6 GKIPPEVLIKIIDGIKPGERVIVGPGVGIDATAIDFGDYVLVASTDPITGAEERIGFYSV 65
GK+P + K I + G+ + P +G DA ++ D L+ +DPIT A+ GF S+
Sbjct: 29 GKVP---INKFIYNLPIGD-CLTCPSIGEDAAVLEAKDKYLIVHSDPITEAKSDSGFLSI 84
Query: 66 HVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEA---MGIAIVGGHTEV 122
V ND+ GA+ +W IL+ DI+++ A I +SEA +G +VGGHTEV
Sbjct: 85 AVACNDINMKGAQCRWVNSIILL------SDISHLNATISAISEACNLIGCKVVGGHTEV 138
Query: 123 TPGLKRPIVVGTMLGEVDRDNLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEEL--RGV 180
+ +K+ I++ T +G V L + K G +++ GIEGT I+A + ++ L +G+
Sbjct: 139 SDKVKQDIIITTAIG-VSNKFLSYKNVKEGMKVVLVGSPGIEGTWILAKDYDQLLLEKGI 197
Query: 181 FGRDFVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDLGFRIFAE 240
R+ +E+AK F ++I + +A+ ++ AMHD TEGGI + E+A + I +
Sbjct: 198 -SREIIEKAKEFKKDIVVQPKALSILDYVVAMHDATEGGIMQAMLEVAKASGYTVSINLD 256
Query: 241 KIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLSYGISASIIGE 296
KI++R ET+ I +++PL LISSG+ I+V K L ER+ A IIGE
Sbjct: 257 KIVLRRETKIITKALNIDPLRLISSGS-FIAVSNTPEKIL-ERI----SEAYIIGE 306
>sp|Q58089|Y676_METJA PUTATIVE HYDROGENASE EXPRESSION/FORMATION PROTEIN MJ0676
>gi|2127980|pir||D64384 hydrogenase expression/formation
protein homolog - Methanococcus jannaschii
>gi|1591391|gb|AAB98671.1| (U67515) hydrogenase
expression/formation protein (hypE) [Methanococcus
jannaschii]
Length = 335
Score = 143 bits (357), Expect = 3e-33
Identities = 104/325 (32%), Positives = 169/325 (52%), Gaps = 23/325 (7%)
Query: 1 MEMLAGKIPPEVLIKIIDGIKPGERVIVGPGVGI----DATAIDFGDYVLVASTD----- 51
M GK+ E++ +I +K E V G+G+ D+ I GD +V + D
Sbjct: 6 MHGAGGKVMQELIKDVI--LKNLEITSVNGGIGLESLDDSATIPIGDKEIVFTVDGHTVK 63
Query: 52 PITGAEERIGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAM 111
PI IG +V ND+A GAKP +++++PEG + ED+ I+ I E S+
Sbjct: 64 PIFFPGGDIGRLAVSGTVNDLAVMGAKPLALSLSLIIPEGFNLEDLEKIVKSINETSKEA 123
Query: 112 GIAIVGGHTEVTPGLKRPIVVGTMLGEVDRDNLVVP-DPKPGDAIIMTKGAGIEGTSIIA 170
+AI+ G T+V+ G+ I+ +G VDR + + + GDAII++ G G +I+
Sbjct: 124 EVAIITGDTKVSDGVDDIIISTAGIGIVDRGKAIRDCNVQEGDAIIVSGNIGEHGLAILL 183
Query: 171 HEREEELRGVFGRDFVERAKAFIEEISIVREAMIAREFA-TAMHDPTEGGIANGIHEMAD 229
G DF K+ + I+ + E ++ AM DPT GG+A+ ++EMA+
Sbjct: 184 SRE--------GFDFETNIKSDVAPINKLIERVLEEGIQINAMKDPTRGGLADALNEMAE 235
Query: 230 INDLGFRIFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLSY-- 287
+++G IF +KI I DE + ICD L+PL + + G ++++V ++ A+ +E L +
Sbjct: 236 KSNIGITIFEDKIPISDEVQSICDILGLDPLTIANEGKVVMAVKKEDAERCLEILREHPL 295
Query: 288 GISASIIGEFLAEKKRVIIENGIER 312
G +A IIG E K VIIE + R
Sbjct: 296 GKNAEIIGYATKEHKGVIIETIVGR 320
>pir||B69125 hydrogenase expression/formation protein HypE - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2621250|gb|AAB84711.1| (AE000807) hydrogenase
expression/formation protein HypE [Methanobacterium
thermoautotrophicum]
Length = 376
Score = 126 bits (313), Expect = 4e-28
Identities = 97/314 (30%), Positives = 158/314 (49%), Gaps = 25/314 (7%)
Query: 28 VGPGVGI----DATAIDFGDYVLVASTD-----PITGAEERIGFYSVHVNANDVATFGAK 78
V GVG+ D +I GDY +V STD P+ IG +V ND++ GA+
Sbjct: 71 VNGGVGLEDLDDGASIPLGDYEIVISTDGHTIDPLFFPGGDIGRIAVAGTVNDISVMGAR 130
Query: 79 PKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGHTEVTPG--LKRPIVVGTML 136
P +++ EG ED+ I+ + VS G++IV G T+V L R ++ T +
Sbjct: 131 PLAIANAMIISEGFPGEDLERIIKSMDAVSRETGVSIVTGDTKVMEQGKLDRMVITTTGI 190
Query: 137 GEVDRDNLVVPDP-KPGDAIIMTKGAGIEGTSIIAHEREEELRGVFGRDFVERAKAFIEE 195
G V R V +PGD II+T G G +++A G F ++ +
Sbjct: 191 GVVGRGETVRDSGLRPGDKIILTGSVGDHGMALMAFRE--------GFGFDTDLESDVAP 242
Query: 196 ISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDLGFRIFAEKIIIRDETRKICDFY 255
+ + EA + TAM DPT GGIAN ++EMAD + +G + + I +R+E + + +
Sbjct: 243 VWGIVEAALNVGGVTAMKDPTRGGIANALNEMADKSGVGMVLDEDSIPVREEVKAVSEML 302
Query: 256 DLNPLALISSGALLISVPRDHAKFLVERLLS--YGISASIIGEFLAEKKRVIIEN--GIE 311
++P + + G ++I V ++A+ ++ + S YG A IIGE + + K VI+E G
Sbjct: 303 GIDPYEVANEGKVIIGVDPEYAEDVLAAVRSAPYGEEARIIGE-VTDDKHVILETSLGGR 361
Query: 312 RPLKRPESDELWKV 325
R L+ P +D + +V
Sbjct: 362 RILEAPVADPVPRV 375
>gb|AAG18782.1| (AE004983) hydrogenase expression/formation; Hef [Halobacterium sp.
NRC-1]
Length = 339
Score = 114 bits (283), Expect = 1e-24
Identities = 84/306 (27%), Positives = 143/306 (46%), Gaps = 14/306 (4%)
Query: 26 VIVGPGVGIDATAIDFGDYVLVASTDPITGAE----ERIGFYSVHVNANDVATFGAKPKW 81
V +GP G D + D V+ +TDP+ ER +++ H+ +DVA G P
Sbjct: 26 VRLGPTYGADFGVVAIADSVVAMATDPVFVLRALGIERAAWFAFHICVSDVALSGLSPAH 85
Query: 82 FLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGHTEVTPGLKRPIVVG-TMLGEVD 140
V + +P ++ E I + A+G+++ GHT G P V G T L
Sbjct: 86 LAVDLNLPPDTATETFDAIWEVFDREARALGVSLTTGHTGTYEGCSFPTVGGATALAVGA 145
Query: 141 RDNLVVPD-PKPGDAIIMTKGAGIEGTSIIAHEREEELRGVFGRDFVERAKAFIEEISIV 199
+++V P +PGD +++TKG +E T ++ + L A+ + + V
Sbjct: 146 AEDVVTPTGAQPGDRVVVTKGPAVETTGMLGVLFGDRLP--ISAAAAAAARDRFADATPV 203
Query: 200 REA--MIAREFATAMHDPTEGGIANGIHEMADINDLGFRIFAEKIIIRDETRKICDFYDL 257
R+A M A TAMHD TE G+ANG+HE+A + +G + + + + R +C+ + +
Sbjct: 204 RDAATMAAAGEVTAMHDATERGLANGLHELAAASGVGLSVARDAVPVLPGVRAVCEQFGM 263
Query: 258 NPLALISSGALLISVPRDHAKFLVERLLSYGISASIIGEFLAEKKRVIIENGIERPLKRP 317
+ A S G ++ +V ++ L GI A+ +G + E + +G PL P
Sbjct: 264 DAWAASSEGTVIAAVDPGSVDAVLGALGDEGIPAADVG--VVEAGSGVTVDG--DPLAEP 319
Query: 318 ESDELW 323
+D LW
Sbjct: 320 AADPLW 325
>pir||A81411 hydrogenase isoenzymes formation protein Cj0626 [imported] -
Campylobacter jejuni (strain NCTC 11168)
>gi|6968093|emb|CAB75262.1| (AL139075) hydrogenase
isoenzymes formation protein [Campylobacter jejuni]
Length = 324
Score = 113 bits (281), Expect = 2e-24
Identities = 88/278 (31%), Positives = 139/278 (49%), Gaps = 23/278 (8%)
Query: 57 EERIGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIV 116
E IG V + NDV GAKPK+ + +++ EG E + I+ I+E E G+ +V
Sbjct: 60 EVNIGKLCVCGSINDVLMVGAKPKYLSLGLILEEGFELEKLERILKSIKEECEKCGVMLV 119
Query: 117 GGHTEVTP-GLKRPIVVGTM-LGEV----DRDNLVVPDPKPGDAIIMTKGAGIEGTSIIA 170
G T+V P G I + T LGE+ + N+ K G +I+++ G G S++
Sbjct: 120 CGDTKVVPKGKADEIYINTTALGEIISKKESKNI-----KAGLSILLSGDIGRHGASVLI 174
Query: 171 HEREEELRGVFGRDFVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADI 230
E E D KA +E+ + E I AM D T GG++ ++E A
Sbjct: 175 KRNELEA------DIKSDCKALNKEVLELLEKDIK---VVAMRDATRGGLSAVLNEWAKQ 225
Query: 231 NDLGFRIFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLSYGIS 290
+ IF EKII++DE +C+ + L + G ++ V ++ +E L Y ++
Sbjct: 226 SGNDLLIFEEKIIVQDEVLGLCELFGYEAYELANEGTFILCVEKEDELKALEILKKYNVN 285
Query: 291 ASIIGEFLAEKK-RVIIEN--GIERPLKRPESDELWKV 325
ASIIGE L EKK RVI++N G +R L+ P+ + L ++
Sbjct: 286 ASIIGEVLEEKKARVILQNAYGAKRFLESPKGELLPRI 323
>gi|11498961 hydrogenase expression/formation protein (hypE) [Archaeoglobus
fulgidus] >gi|7447551|pir||D69420 hydrogenase
expression/formation protein (hypE) homolog -
Archaeoglobus fulgidus >gi|2649205|gb|AAB89877.1|
(AE001009) hydrogenase expression/formation protein
(hypE) [Archaeoglobus fulgidus]
Length = 344
Score = 107 bits (265), Expect = 2e-22
Identities = 85/286 (29%), Positives = 143/286 (49%), Gaps = 30/286 (10%)
Query: 60 IGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGH 119
IG SV +ND+A GAKP++ + +++ +G D IM +IR+ E + ++I+ G
Sbjct: 68 IGSLSVCGTSNDLAVMGAKPEFMSLALVIQDGFPLGDFRRIMDDIRDSVELVNLSIITGD 127
Query: 120 TEVTPGLKRPIV----VGTMLGEVDRDNLVVPDP-------------KPGDAIIMTKGAG 162
T+V IV +G E++R+ V+ + K GDAII++ G+
Sbjct: 128 TKVVESNVGIIVNTAGIGRRSEELERNLEVLRELRDYPYRWVRDKGLKEGDAIIVS-GSI 186
Query: 163 IEGTSIIAHEREEELRGVFGRDFVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIAN 222
E ++I ER F K+ + + + + R TAM DPT GGIA
Sbjct: 187 AEHAAVIMAER---------MGFEVEFKSDVYPVWLFVREALRRGGVTAMKDPTRGGIAA 237
Query: 223 GIHEMADINDLGFRIFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVE 282
++EMA+ + +G +IF E+I ++DE R +C+ L+PL + + G ++ V + A +++
Sbjct: 238 ALNEMAEKSGVGIKIFEERIPLKDEVRGLCELLGLDPLTMANEGKVVFGVHPEMADEVLK 297
Query: 283 RLLSYG-ISASIIGEFLAEKKRVIIEN--GIERPLKRPESDELWKV 325
L G A IIG E V++E G R + +P SD + +V
Sbjct: 298 ALHKAGQKEAEIIGYATKEFAEVVLETAVGTTRVVPQPISDPIPRV 343
>sp|P40595|HYPE_AZOVI HYDROGENASE EXPRESSION/FORMATION PROTEIN HYPE
>gi|628532|pir||S43354 hypE protein - Azotobacter
vinelandii >gi|398012|gb|AAA19513.1| (L23970) hypE
[Azotobacter vinelandii]
Length = 341
Score = 95.6 bits (234), Expect = 7e-19
Identities = 68/251 (27%), Positives = 118/251 (46%), Gaps = 12/251 (4%)
Query: 60 IGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGH 119
IG +VH NDVA GA+P + ++ EG D+A I+ + + G+ +V G
Sbjct: 74 IGSLAVHGTINDVAMAGARPCYLAAGFILEEGFPLADLARIVESMAAAAREAGVPVVTGD 133
Query: 120 TEVTPGLKRP--IVVGTMLGEVDRD-NLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEE 176
T+V K + T +G V +L +PGD I+++ G G +I++
Sbjct: 134 TKVVEHGKGDGVFITTTGVGVVPPGLHLSGDQARPGDRILLSGSIGDHGVTILSLREGLG 193
Query: 177 LRGVFGRDFVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDLGFR 236
G D ++ V E R DPT GG+ N ++E+A + +G +
Sbjct: 194 FEADIGSDSQALHGLVAAMLAAVPEIRCLR-------DPTRGGLGNTLNELARQSGVGMQ 246
Query: 237 IFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLSY--GISASII 294
+ I +R+ R C+F L+PL + + G L+ P + A+ L+E + ++ G A+II
Sbjct: 247 LVERAIPVREPVRAACEFLGLDPLYVANEGKLIAICPAERAERLLEAMRAHPQGREAAII 306
Query: 295 GEFLAEKKRVI 305
G +A++ R +
Sbjct: 307 GTVVADEHRFV 317
>pir||E75040 hydrogenase expression/formation protein (hype) PAB0868 -
Pyrococcus abyssi (strain Orsay)
>gi|5458731|emb|CAB50218.1| (AJ248287) hydrogenase
expression/formation protein (hypE) [Pyrococcus abyssi]
Length = 336
Score = 94.8 bits (232), Expect = 1e-18
Identities = 76/274 (27%), Positives = 138/274 (49%), Gaps = 17/274 (6%)
Query: 60 IGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGH 119
IG +V ND+A GAKP ++++ EG +D+ I+ + E ++ + + IV G
Sbjct: 71 IGRLAVSGTVNDLAVMGAKPIALASSLIIGEGFDSQDLERILKSMDETAKEVPVPIVTGD 130
Query: 120 TEVTPGLKRPIVVGTMLGEVDRDNLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEELRG 179
T+V V+ +G ++ + K GDA++++ G G ++++H
Sbjct: 131 TKVVEENVGIFVITAGIGIAEKP-ISDAGAKVGDAVLVSGTIGDHGIALMSHRE------ 183
Query: 180 VFGRDFVERAKAFIEEISIVREAM---IAREFATAMHDPTEGGIANGIHEMADINDLGFR 236
G F K+ + V +A+ I E AM DPT GG++N ++EMA ++G
Sbjct: 184 --GISFETELKSDVAPTWDVVKAVADAIGWENIHAMKDPTRGGLSNALNEMARKANVGIL 241
Query: 237 IFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVE--RLLSYGISASII 294
I +I IR E + + ++P + + G +++ V R++A+ +E R G +A+II
Sbjct: 242 IKESEIPIRPEVKAASEMLGISPYEVANEGKVVMIVEREYAEEALEAMRKTEKGRNAAII 301
Query: 295 GEFLAE-KKRVIIENGI--ERPLKRPESDELWKV 325
GE + + +VI+E GI +R L+ P D + +V
Sbjct: 302 GEVIKDYPGKVILETGIGGKRFLEPPIGDPVPRV 335
>gb|AAB64436.1| (AF006594) HupE [Anabaena PCC7120]
Length = 372
Score = 92.8 bits (227), Expect = 5e-18
Identities = 69/265 (26%), Positives = 129/265 (48%), Gaps = 17/265 (6%)
Query: 51 DPITGAEERIGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVS-- 108
DP+ IG +++ ND+A GAKP +F ++++ EG E + + ++ S
Sbjct: 91 DPLFFPGSDIGELAINGTINDLAVSGAKPLYFTCSVILEEGLPIETLRRVATSMKVPSMK 150
Query: 109 ---EAMGIAIVGGHTEVTP-GLKRPIVVGTM-LGEVDRD-NLVVPDPKPGDAIIMTKGAG 162
+ G+ IV G T+V P G + + T +G + ++ + +PGD+II+ G
Sbjct: 151 VAAQKAGVQIVTGDTKVVPRGCADKLFINTAGIGIIPSGIDISAHNIQPGDSIIINGELG 210
Query: 163 IEGTSIIAHEREEELRGVFGRDFVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIAN 222
G +I+ E L D +A + ++ + AM D T GG+A
Sbjct: 211 NHGAAILIARGELALETNIESD----CQALHDLVATILNVCPQ---VHAMRDATRGGLAT 263
Query: 223 GIHEMADINDLGFRIFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVE 282
++E A + +G R++ E + +R+E + +C+ L+PL L + G L++ V + A+ ++
Sbjct: 264 VLNEFATSSHVGIRLYEESLPVREEVKGMCEILGLDPLHLANEGKLVVVVDSEQAQAVLS 323
Query: 283 RLLSY--GISASIIGEFLAEKKRVI 305
+ S+ G A I+GE +A V+
Sbjct: 324 AMKSHPKGQDACIVGEVMATPPGVV 348
>emb|CAA51035.1| (X72294) ORF9 [Azotobacter vinelandii]
Length = 341
Score = 92.4 bits (226), Expect = 6e-18
Identities = 67/251 (26%), Positives = 117/251 (45%), Gaps = 12/251 (4%)
Query: 60 IGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGH 119
IG +VH NDVA GA+P + ++ EG D+A I+ + + G+ +V G
Sbjct: 74 IGSLAVHGTINDVAMAGARPCYLAAGFILEEGFPLADLARIVESMAAAAREAGVPVVTGD 133
Query: 120 TEVTPGLKRP--IVVGTMLGEVDRD-NLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEE 176
T+V K + T +G V +L +PGD I+++ G G +I++
Sbjct: 134 TKVVEHGKGDGVFITTTGVGVVPPGLHLSGDQARPGDRILLSGSIGDHGVTILSLREGLG 193
Query: 177 LRGVFGRDFVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDLGFR 236
G D ++ V E R DPT GG+ N ++E+A + +G +
Sbjct: 194 FEADIGSDSQALHGLVAAMLAAVPEIRCLR-------DPTRGGLGNTLNELARQSGVGMQ 246
Query: 237 IFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLSY--GISASII 294
+ I +R+ R C+F L+PL + + G L+ + A+ L+E + ++ G A+II
Sbjct: 247 LVERAIPVREPVRAACEFLGLDPLYVANEGKLIAICAAERAERLLEAMRAHPQGREAAII 306
Query: 295 GEFLAEKKRVI 305
G +A++ R +
Sbjct: 307 GTVVADEHRFV 317
>pir||S35767 hypothetical protein 9 - Azotobacter vinelandii
Length = 329
Score = 92.4 bits (226), Expect = 6e-18
Identities = 67/251 (26%), Positives = 117/251 (45%), Gaps = 12/251 (4%)
Query: 60 IGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGH 119
IG +VH NDVA GA+P + ++ EG D+A I+ + + G+ +V G
Sbjct: 62 IGSLAVHGTINDVAMAGARPCYLAAGFILEEGFPLADLARIVESMAAAAREAGVPVVTGD 121
Query: 120 TEVTPGLKRP--IVVGTMLGEVDRD-NLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEE 176
T+V K + T +G V +L +PGD I+++ G G +I++
Sbjct: 122 TKVVEHGKGDGVFITTTGVGVVPPGLHLSGDQARPGDRILLSGSIGDHGVTILSLREGLG 181
Query: 177 LRGVFGRDFVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDLGFR 236
G D ++ V E R DPT GG+ N ++E+A + +G +
Sbjct: 182 FEADIGSDSQALHGLVAAMLAAVPEIRCLR-------DPTRGGLGNTLNELARQSGVGMQ 234
Query: 237 IFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLSY--GISASII 294
+ I +R+ R C+F L+PL + + G L+ + A+ L+E + ++ G A+II
Sbjct: 235 LVERAIPVREPVRAACEFLGLDPLYVANEGKLIAICAAERAERLLEAMRAHPQGREAAII 294
Query: 295 GEFLAEKKRVI 305
G +A++ R +
Sbjct: 295 GTVVADEHRFV 305
>pir||A70388 hydrogenase expression/formation protein - Aquifex aeolicus
>gi|2983521|gb|AAC07097.1| (AE000719) hydrogenase
expression/formation protein [Aquifex aeolicus]
Length = 332
Score = 92.4 bits (226), Expect = 6e-18
Identities = 68/274 (24%), Positives = 140/274 (50%), Gaps = 16/274 (5%)
Query: 60 IGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGH 119
IG +V ND++ GA+PK+ V+ ++ EG S E++ ++ + + + G+ IV G
Sbjct: 66 IGKLAVAGTVNDLSVMGARPKYLSVSFVIEEGLSFEELEEVVKSMAQEASKTGVQIVAGD 125
Query: 120 TEVTP--GLKRPIVVGTMLGEVDRDNLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEEL 177
T+V P + + + +GEV + L + + G+ II++ G G I+A +RE
Sbjct: 126 TKVVPKGQADKLFISASGIGEVVYEGLSAHNLREGNVIIVSGSVGDHGACILA-QRE--- 181
Query: 178 RGVFGRDFVERAKAFIEEI-SIVREAMIAREFATAMHDPTEGGIANGIHEMADINDLGFR 236
G +F ++ + + +++ + + + A+ D T GG+A ++E A+ + +
Sbjct: 182 ----GMEFEVPVESDCKSLWNMIEKVLSSGAEVHAIRDATRGGLAGVLNEWAEQSKVTIE 237
Query: 237 IFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLS--YGISASII 294
+ EKI +++ R +C+ P L + G + +V A+ ++E L S G A II
Sbjct: 238 VEEEKIPVKEAVRGLCELLGFEPTHLANEGMAVFAVASQDAQKVLEILRSTPEGKEAQII 297
Query: 295 GEFLAE-KKRVIIEN--GIERPLKRPESDELWKV 325
G+ + + + +VI+ G++R ++ P + L ++
Sbjct: 298 GKVVKKGEPKVILSTPYGVKRVMEPPAGELLPRI 331
>sp|P42034|HUPE_AZOCH HYDROGENASE EXPRESSION/FORMATION PROTEIN HUPE
>gi|2120753|pir||I39744 hydrogenase homolog 2 -
Azotobacter chroococcum >gi|142322|gb|AAA22137.1|
(M92282) hydrogenase-related protein [Azotobacter
chroococcum] >gi|382216|prf||1819270B hydrogenase
[Azotobacter chroococcum]
Length = 341
Score = 92.1 bits (225), Expect = 8e-18
Identities = 67/249 (26%), Positives = 116/249 (45%), Gaps = 18/249 (7%)
Query: 60 IGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGH 119
IG +VH NDVA GA+P + ++ EG D+A I+ + + G+ +V G
Sbjct: 74 IGSLAVHGTINDVAMAGARPCYLAAGFILEEGFPLADLARIVESMAAAAREAGVPVVTGD 133
Query: 120 TEVTPGLKRP--IVVGTMLGEVDRDNLVVPD-PKPGDAIIMTKGAGIEGTSIIAHEREEE 176
T+V K + T +G V + D +PGD I+++ G G +I++
Sbjct: 134 TKVVERGKGDGVFITTTGVGVVPPGLYLSGDRARPGDRILLSGSIGDHGVTILSLREGLG 193
Query: 177 LRGVFGRDFVER---AKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDL 233
G D A +E + +R + DPT GG+ N ++E+A + +
Sbjct: 194 FEADIGSDSQALHGLVAAMVEAVPEIR----------CLRDPTRGGLGNTLNELARQSGV 243
Query: 234 GFRIFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLSY--GISA 291
G ++ I +R+ R C+F L+PL + + G L+ P + A+ L+E + + G A
Sbjct: 244 GMQLVERAIPLREPVRAACEFLGLDPLYVANEGKLIAICPAEQAERLLEVMREHPQGREA 303
Query: 292 SIIGEFLAE 300
+IIG +A+
Sbjct: 304 AIIGTVVAD 312
>pir||D71121 probable hydrogenase expression/formation protein - Pyrococcus
horikoshii >gi|3257147|dbj|BAA29830.1| (AP000003) 336aa
long hypothetical hydrogenase expression/formation
protein [Pyrococcus horikoshii]
Length = 336
Score = 91.7 bits (224), Expect = 1e-17
Identities = 82/312 (26%), Positives = 145/312 (46%), Gaps = 26/312 (8%)
Query: 31 GVGIDA----TAIDFGDYVLVASTD-----PITGAEERIGFYSVHVNANDVATFGAKPKW 81
G+G++A I F LV + D P+ IG +V ND+A GAKP
Sbjct: 33 GIGLEALDDGATIPFEGKHLVFTIDGHTVRPLFFPGGDIGRLAVSGTVNDLAVMGAKPLA 92
Query: 82 FLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGHTEVTPGLKRPIVVGTMLGEVDR 141
++++ EG + + I+ + ++ + + I+ G T+V V+ +G ++
Sbjct: 93 LASSLIIGEGFDSDKLEEILKSMNRTAKEVPVPIITGDTKVVEDNIEIFVITAGIGIAEK 152
Query: 142 DNLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEELRGVFGRDFVERAKAFIEEISIVRE 201
+ K GD +++ G G ++++H G F K+ + I V +
Sbjct: 153 P-ISDAGAKVGDLVLVNGTIGDHGIALMSHRE--------GISFETELKSDVAPIWDVVK 203
Query: 202 AM---IAREFATAMHDPTEGGIANGIHEMADINDLGFRIFAEKIIIRDETRKICDFYDLN 258
+ I E AM DPT GG++N ++EMA D+G + I IR E + D ++
Sbjct: 204 TVADAIGWENIHAMKDPTRGGLSNALNEMARKADVGILVRESDIPIRPEVKAAGDMLGIS 263
Query: 259 PLALISSGALLISVPRDHAKFLVE--RLLSYGISASIIGEFLAE-KKRVIIENGI--ERP 313
P + + G +++ V R++A+ +E R G A+IIGE + + +VI+E GI R
Sbjct: 264 PYDVANEGKVIMVVDREYAEDALEAMRKTEKGKDAAIIGEIIKDYPGKVILETGIGGRRF 323
Query: 314 LKRPESDELWKV 325
L+ P D + +V
Sbjct: 324 LEPPIGDPVPRV 335
>sp|P40599|HYPE_RHILV HYDROGENASE EXPRESSION/FORMATION PROTEIN HYPE
>gi|420901|pir||S32878 hydrogenase expression/formation
protein hypE [validated] - Rhizobium leguminosarum bv.
viciae >gi|48737|emb|CAA37164.1| (X52974) hydrophobic
protein [Rhizobium leguminosarum]
>gi|536795|emb|CAA85445.1| (Z36982) HYPE [Rhizobium
leguminosarum]
Length = 350
Score = 89.7 bits (219), Expect = 4e-17
Identities = 77/278 (27%), Positives = 128/278 (45%), Gaps = 16/278 (5%)
Query: 33 GIDATAIDFGDYVLVASTD-----PITGAEERIGFYSVHVNANDVATFGAKPKWFLVTIL 87
G D +A +V +TD P+ IG +VH ND+A GA P + + +
Sbjct: 50 GNDQSAFSVPGGRMVMTTDGYVVSPLFFPGGNIGTLAVHGTINDIAMAGAVPLYLSASFI 109
Query: 88 MPEGSSEEDIANIMAEIREVSEAMGIAIVGGHTEVTPGLKRPIVVGTMLG---EVDRDNL 144
+ EG D+ I + S G+ I+ G T+V K V + G D +L
Sbjct: 110 IEEGFPLVDLERIADSMGAASREAGVPIITGDTKVVERGKADGVFISTAGIGMAPDGLDL 169
Query: 145 VVPDPKPGDAIIMTKGAGIEGTSIIAHEREEELRGVFGRDFVERAKAFIEEISIVREAMI 204
+PGDA+I++ G G ++++ E F D V + A ++ + A
Sbjct: 170 RSDAARPGDAVIISGSIGDHGVAVMSKRENLE----FDTDIVSDSAALHGLVADMVAAGG 225
Query: 205 AREFATAMHDPTEGGIANGIHEMADINDLGFRIFAEKIIIRDETRKICDFYDLNPLALIS 264
A M DPT GGIA ++E+A + +GFRI E I ++ E C+F L+PL + +
Sbjct: 226 AH--IRLMRDPTRGGIAATLNEIASQSRVGFRIDEEAIPMKPEVAAACEFLGLDPLNVAN 283
Query: 265 SGALLISVPRDHAKFLVERLLSY--GISASIIGEFLAE 300
G L+ V + A ++ + ++ G A++IG +A+
Sbjct: 284 EGKLVAVVAPEGADAVLAAIHAHPLGAEAALIGHVVAD 321
>sp|P24193|HYPE_ECOLI HYDROGENASE ISOENZYMES FORMATION PROTEIN HYPE >gi|95947|pir||S15201
hydrogenase expression/formation protein hypE
[validated] - Escherichia coli >gi|41779|emb|CAA38416.1|
(X54543) hypE product [Escherichia coli]
>gi|1789085|gb|AAC75772.1| (AE000356) plays structural
role in maturation of all 3 hydrogenases [Escherichia
coli K12]
Length = 322
Score = 82.7 bits (201), Expect = 5e-15
Identities = 76/298 (25%), Positives = 136/298 (45%), Gaps = 20/298 (6%)
Query: 35 DATAIDFGDYVLVASTDPITGAEERIGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSE 94
D A YV+ DP+ IG ++ ANDVA GA P++ ++ EG
Sbjct: 37 DRLAFSTDSYVI----DPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEEGLPM 92
Query: 95 EDIANIMAEIREVSEAMGIAIVGGHTEVTP-GLKRPIVVGTM-LGEVDRD-NLVVPDPKP 151
E + ++ + E + A GIAIV G T+V G + + T +G + + +
Sbjct: 93 ETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWGAQTLTA 152
Query: 152 GDAIIMTKGAGIEGTSIIAHEREEELRGVFGRDFVERAKAFIEEISIVREAMIAREFATA 211
GD ++++ G G +I+ + L G + V I +R+ + A
Sbjct: 153 GDVLLVSGTLGDHGATILNLREQLGLDG----ELVSDCAVLTPLIQTLRDIPGVK----A 204
Query: 212 MHDPTEGGIANGIHEMADINDLGFRIFAEKIIIRDETRKICDFYDLNPLALISSGALLIS 271
+ D T GG+ +HE A G + + ++ R +C+ L+ L + G L+I+
Sbjct: 205 LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLVIA 264
Query: 272 VPRDHAKFLVERLLSY--GISASIIGEFLAEKKRVIIEN--GIERPLKRPESDELWKV 325
V R+ A+ ++ L S+ G A++IGE + E+K V + G++R L P ++ L ++
Sbjct: 265 VERNAAEQVLAALHSHPLGKDAALIGE-VVERKGVRLAGLYGVKRTLDLPHAEPLPRI 321
>gb|AAA69240.1| (U29579) CG Site no. 33119; ORF_o322 [Escherichia coli]
Length = 322
Score = 82.7 bits (201), Expect = 5e-15
Identities = 76/298 (25%), Positives = 136/298 (45%), Gaps = 20/298 (6%)
Query: 35 DATAIDFGDYVLVASTDPITGAEERIGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSE 94
D A YV+ DP+ IG ++ ANDVA GA P++ ++ EG
Sbjct: 37 DRLAFSTDSYVI----DPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEEGLPM 92
Query: 95 EDIANIMAEIREVSEAMGIAIVGGHTEVTP-GLKRPIVVGTM-LGEVDRD-NLVVPDPKP 151
E + ++ + E + A GIAIV G T+V G + + T +G + + +
Sbjct: 93 ETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWGAQTLTA 152
Query: 152 GDAIIMTKGAGIEGTSIIAHEREEELRGVFGRDFVERAKAFIEEISIVREAMIAREFATA 211
GD ++++ G G +I+ + L G + V I +R+ + A
Sbjct: 153 GDVLLVSGTLGDHGATILNLREQLGLDG----ELVSDCAVLTPLIQTLRDIPGVK----A 204
Query: 212 MHDPTEGGIANGIHEMADINDLGFRIFAEKIIIRDETRKICDFYDLNPLALISSGALLIS 271
+ D T GG+ +HE A G + + ++ R +C+ L+ L + G L+I+
Sbjct: 205 LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLVIA 264
Query: 272 VPRDHAKFLVERLLSY--GISASIIGEFLAEKKRVIIEN--GIERPLKRPESDELWKV 325
V R+ A+ ++ L S+ G A++IGE + E+K V + G++R L P ++ L ++
Sbjct: 265 VERNAAEQVLAALHSHPLGKDAALIGE-VVERKGVRLAGLYGVKRTLDLPHAEPLPRI 321
>gb|AAG20935.1| (AE005160) hydrogenase expression/formation protein; HypE
[Halobacterium sp. NRC-1]
Length = 231
Score = 80.4 bits (195), Expect = 3e-14
Identities = 64/220 (29%), Positives = 104/220 (47%), Gaps = 10/220 (4%)
Query: 109 EAMGIAIVGGHTEVTPGLKRPIVVG-TMLGEVDRDNLVVPD-PKPGDAIIMTKGAGIEGT 166
E +G+++V GHT + P + G T +G D +V PD GD ++MT G E
Sbjct: 6 ERLGVSVVTGHT-ARQDVSFPWIGGATAMGVGDPAEIVRPDGATAGDTVLMTHSPGAEVA 64
Query: 167 SIIAHEREEELRGVFGRDFVERAKAFIEEISIVREAMIAREF-ATAMHDPTEGGIANGIH 225
+ A + L D VERA A +E+ ++V +A A TAMHD TE GI +
Sbjct: 65 GLFAALFGDHLD--VADDTVERADARLEDTTVVGDACAAASAPVTAMHDATECGIQGALV 122
Query: 226 EMADINDLGFRIFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLL 285
E+A + + ++ + + ++ +C L+P + S G LL++VP HA + L
Sbjct: 123 ELATAAGVSISVSSDAVPVPEDVSAVCADLGLDPWQVSSRGTLLVTVPEAHADTAMAALD 182
Query: 286 SYGISASIIGEFLAEKKRVIIENGIERPLKRPESDELWKV 325
+ + IG ++E V ++ + P SD W V
Sbjct: 183 ARNTPVAAIGT-VSEGSGVTVDG---TQVVHPGSDPAWTV 218
>pir||S35230 hypE protein - Bradyrhizobium japonicum >gi|39536|emb|CAA78989.1|
(Z17373) hypE [Bradyrhizobium japonicum]
>gi|448283|prf||1916405B hypE gene [Bradyrhizobium
japonicum]
Length = 321
Score = 77.3 bits (187), Expect = 2e-13
Identities = 69/278 (24%), Positives = 123/278 (43%), Gaps = 16/278 (5%)
Query: 33 GIDATAIDFGDYVLVASTD-----PITGAEERIGFYSVHVNANDVATFGAKPKWFLVTIL 87
G D +A D +V +TD P+ IG +VH ND+A GAKP + + +
Sbjct: 21 GNDQSAFDVRAGRMVMTTDGYVVSPLFFPGGNIGSLAVHGTVNDIAMAGAKPLYLSASFI 80
Query: 88 MPEGSSEEDIANIMAEIREVSEAMGIAIVGGHTEVTP-GLKRPIVVGTMLGEVDRDNLVV 146
+ EG D+ I + + + I+ G T+V G + + T V D L +
Sbjct: 81 IEEGFRFADLKLIAESMGAAAREADVHIITGDTKVVERGKADGLFISTAGVGVVPDGLDL 140
Query: 147 PDPKP--GDAIIMTKGAGIEGTSIIAHEREEELRGVFGRDFVERAKAFIEEISIVREAMI 204
K GD ++++ G G +I++ + D A + ++ + R
Sbjct: 141 SAEKARVGDRVLISGTLGDHGVAIMSKRQNLAFETEIVSD-----SASLHDL-VARMVQA 194
Query: 205 AREFATAMHDPTEGGIANGIHEMADINDLGFRIFAEKIIIRDETRKICDFYDLNPLALIS 264
M DPT GG+A ++E+A ++LGF + E I ++ C+ L+PL + +
Sbjct: 195 GGRGIRLMRDPTRGGLAATLNEIAQQSNLGFHLLEEAIPVKPGVAAACELLGLDPLHVAN 254
Query: 265 SGALLISVPRDHAKFLVERLLSY--GISASIIGEFLAE 300
G L+ V + A ++ + ++ G A+ IGE +A+
Sbjct: 255 EGKLVAIVAPEAAGAVLAAMRAHPLGRDAADIGEAVAD 292
>sp|P31906|HYPE_BRAJA HYDROGENASE EXPRESSION/FORMATION PROTEIN HYPE
Length = 346
Score = 77.3 bits (187), Expect = 2e-13
Identities = 69/278 (24%), Positives = 123/278 (43%), Gaps = 16/278 (5%)
Query: 33 GIDATAIDFGDYVLVASTD-----PITGAEERIGFYSVHVNANDVATFGAKPKWFLVTIL 87
G D +A D +V +TD P+ IG +VH ND+A GAKP + + +
Sbjct: 46 GNDQSAFDVRAGRMVMTTDGYVVSPLFFPGGNIGSLAVHGTVNDIAMAGAKPLYLSASFI 105
Query: 88 MPEGSSEEDIANIMAEIREVSEAMGIAIVGGHTEVTP-GLKRPIVVGTMLGEVDRDNLVV 146
+ EG D+ I + + + I+ G T+V G + + T V D L +
Sbjct: 106 IEEGFRFADLKLIAESMGAAAREADVHIITGDTKVVERGKADGLFISTAGVGVVPDGLDL 165
Query: 147 PDPKP--GDAIIMTKGAGIEGTSIIAHEREEELRGVFGRDFVERAKAFIEEISIVREAMI 204
K GD ++++ G G +I++ + D A + ++ + R
Sbjct: 166 SAEKARVGDRVLISGTLGDHGVAIMSKRQNLAFETEIVSD-----SASLHDL-VARMVQA 219
Query: 205 AREFATAMHDPTEGGIANGIHEMADINDLGFRIFAEKIIIRDETRKICDFYDLNPLALIS 264
M DPT GG+A ++E+A ++LGF + E I ++ C+ L+PL + +
Sbjct: 220 GGRGIRLMRDPTRGGLAATLNEIAQQSNLGFHLLEEAIPVKPGVAAACELLGLDPLHVAN 279
Query: 265 SGALLISVPRDHAKFLVERLLSY--GISASIIGEFLAE 300
G L+ V + A ++ + ++ G A+ IGE +A+
Sbjct: 280 EGKLVAIVAPEAAGAVLAAMRAHPLGRDAADIGEAVAD 317
>sp|P31905|HYPE_ALCEU HYDROGENASE EXPRESSION/FORMATION PROTEIN HYPE
>gi|321750|pir||S29979 hypE protein - Alcaligenes
eutrophus >gi|38776|emb|CAA49735.1| (X70183) HYPE
[Ralstonia eutropha]
Length = 351
Score = 76.9 bits (186), Expect = 3e-13
Identities = 62/251 (24%), Positives = 109/251 (42%), Gaps = 12/251 (4%)
Query: 60 IGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGH 119
IG SVH NDVA GAKP + + ++ EG D+ I+ + + G+ IV G
Sbjct: 84 IGSLSVHGTINDVAMAGAKPLYLAASFILEEGFPLADLKRIVESMAGAAREAGVPIVTGD 143
Query: 120 TEVTPGLKRP--IVVGTMLGEVDRDNLV-VPDPKPGDAIIMTKGAGIEGTSIIAHEREEE 176
T+V K + T +G V L+ +PGDAI+++ G G +I++ E
Sbjct: 144 TKVVEQGKGDGVFITTTGVGVVPAGILIDGAGARPGDAILLSGTMGEHGVAILSKRESLE 203
Query: 177 LRGVFGRDFVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDLGFR 236
D + +++V + R DPT GG+A ++E++ + +G
Sbjct: 204 FDTEIRSDSAALHDLVAQMLAVVPGVRVLR-------DPTRGGLATTLNEISSQSGVGMV 256
Query: 237 IFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLSY--GISASII 294
+ I + + C+ L+PL + + G L+ A L+ + + G A I
Sbjct: 257 LDEAAIPVLPQVDAACELLGLDPLYVANEGKLVAICAAADADALLAAMRGHPLGREARRI 316
Query: 295 GEFLAEKKRVI 305
GE + + + +
Sbjct: 317 GEVIEDGRHFV 327
>pir||S21906 hypE protein - Rhodobacter capsulatus >gi|46056|emb|CAA43329.1|
(X61007) HypE [Rhodobacter capsulatus]
Length = 322
Score = 76.9 bits (186), Expect = 3e-13
Identities = 65/246 (26%), Positives = 106/246 (42%), Gaps = 12/246 (4%)
Query: 60 IGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGH 119
IG +V ND+A GA+P W ++ EG+ + I+A + +EA G+ IV G
Sbjct: 55 IGKIAVCGTVNDLAVGGARPLWLSAAFILEEGTEVALLRRIVATMAREAEAAGVRIVTGD 114
Query: 120 TEVT-PGLKRPIVVGTM-LGEVDRD-NLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEE 176
T+V G + V T +G + + +PGD I+ G G +I+A +
Sbjct: 115 TKVVGRGACDGVFVTTSGVGVIPPGREMAAGRVRPGDVAIVNGVLGDHGATILAARGDLA 174
Query: 177 LRGVFGRDFVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDLGFR 236
L D ++ + + A A D T GG+A+ ++E+A G
Sbjct: 175 LTSDIESDCAALG-------HLMADVIAAAPGIRAARDLTRGGLASALNEIAQTAGCGLV 227
Query: 237 IFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDH--AKFLVERLLSYGISASII 294
I + +R E +C+ L+PL L + G L++ VP A R G A ++
Sbjct: 228 IEETALPLRPEVVGLCEILGLDPLYLANEGRLVVFVPEAEAAAALAAMRARPEGAGACVV 287
Query: 295 GEFLAE 300
G +AE
Sbjct: 288 GRAVAE 293
>sp|P26412|HYPE_RHOCA HYDROGENASE EXPRESSION/FORMATION PROTEIN HYPE
>gi|2126468|pir||S32953 hypE protein - Rhodobacter
capsulatus
Length = 340
Score = 76.9 bits (186), Expect = 3e-13
Identities = 65/246 (26%), Positives = 106/246 (42%), Gaps = 12/246 (4%)
Query: 60 IGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGH 119
IG +V ND+A GA+P W ++ EG+ + I+A + +EA G+ IV G
Sbjct: 73 IGKIAVCGTVNDLAVGGARPLWLSAAFILEEGTEVALLRRIVATMAREAEAAGVRIVTGD 132
Query: 120 TEVT-PGLKRPIVVGTM-LGEVDRD-NLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEE 176
T+V G + V T +G + + +PGD I+ G G +I+A +
Sbjct: 133 TKVVGRGACDGVFVTTSGVGVIPPGREMAAGRVRPGDVAIVNGVLGDHGATILAARGDLA 192
Query: 177 LRGVFGRDFVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDLGFR 236
L D ++ + + A A D T GG+A+ ++E+A G
Sbjct: 193 LTSDIESDCAALG-------HLMADVIAAAPGIRAARDLTRGGLASALNEIAQTAGCGLV 245
Query: 237 IFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDH--AKFLVERLLSYGISASII 294
I + +R E +C+ L+PL L + G L++ VP A R G A ++
Sbjct: 246 IEETALPLRPEVVGLCEILGLDPLYLANEGRLVVFVPEAEAAAALAAMRARPEGAGACVV 305
Query: 295 GEFLAE 300
G +AE
Sbjct: 306 GRAVAE 311
>pir||S77375 hydrogenase expression/formation protein hypE - Synechocystis sp.
(strain PCC 6803) >gi|1652557|dbj|BAA17478.1| (D90906)
hydrogenase expression/formation protein HypE
[Synechocystis sp.]
Length = 345
Score = 75.7 bits (183), Expect = 7e-13
Identities = 63/261 (24%), Positives = 117/261 (44%), Gaps = 14/261 (5%)
Query: 60 IGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGH 119
IG +VH ND+A GA P++ V ++ EG E + + + + ++ G+ I+ G
Sbjct: 77 IGSLAVHGTVNDLAMAGATPRYISVGFILEEGLPMETLWRVAQSLGQAAQNCGVEILTGD 136
Query: 120 TEVTP-GLKRPIVVGTM-LGEVDRDNLVVPDP-KPGDAIIMTKGAGIEGTSIIAHEREEE 176
T+V G I + T +G +D + P+ + GD +I++ G G +I+A + E
Sbjct: 137 TKVVDRGKGDGIFINTSGIGSLDHQQTIHPNQVQVGDRLILSGDLGRHGMAIMAVRQGLE 196
Query: 177 LRGVFGRDFVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDLGFR 236
D + E+ + A I + D T GG+A+ ++E+A + +
Sbjct: 197 FETTIESD----SAPVHREVQALLSAGIPIH---CLRDLTRGGLASAVNEIAQTSGVTMA 249
Query: 237 IFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLSYGISASIIGE 296
+ I + E + C+ +PL + + G L VP + + VE L ++ A+ IG
Sbjct: 250 LRETLIPVEAEVQAACELLGFDPLYVANEGRFLAIVPPEAEQKTVEILQTFHPQATAIGT 309
Query: 297 FLAEKKR----VIIENGIERP 313
+ + V +E+ I P
Sbjct: 310 VTGKSAQTLGLVSLESSIGAP 330
>pir||G64525 hydrogenase expression/formation protein - Helicobacter pylori
(strain 26695) >gi|2313121|gb|AAD07114.1| (AE000526)
hydrogenase expression/formation protein (hypE)
[Helicobacter pylori 26695]
Length = 332
Score = 74.5 bits (180), Expect = 2e-12
Identities = 68/277 (24%), Positives = 131/277 (46%), Gaps = 22/277 (7%)
Query: 60 IGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGH 119
IG V +ANDV+ G +P + + ++ EG + I+ I++ + ++
Sbjct: 66 IGKLCVCGSANDVSVQGGEPLYLNMGFILEEGLEISLLKQILQSIQKELFKANLKLLSLD 125
Query: 120 TEVTP--GLKRPIVVGTMLGEVDRDNLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEEL 177
T+V P + + + T +G++ + + + G AII++ G S+ A E +L
Sbjct: 126 TKVVPKGSVDKLFINTTCIGKIIKPGISSYHLQQGQAIILSDTIANHGASLFAMRNEIKL 185
Query: 178 RGVFGRD----FVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDL 233
+ D + F+ ++ I A+ D T GG+A+ ++E A+ + +
Sbjct: 186 KTNLESDCQLLYPLLKPLFLSDLKI-----------DALRDATRGGLASVLNEWANSSRV 234
Query: 234 GFRIFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLS--YGISA 291
I EKI +++ET+ IC+ L P AL + G ++++ + A +E L S +A
Sbjct: 235 KIVIEEEKIPLKEETKGICEILGLEPYALANEGVFVLALNQKDAPKALEILKSNEKAKNA 294
Query: 292 SIIGE-FLAEKKRVIIEN--GIERPLKRPESDELWKV 325
+IG+ F V+++N G ER L+ PE + L ++
Sbjct: 295 CVIGKVFENPYPSVVLKNAWGFERILEVPEGELLPRI 331
>gb|AAC26158.1| (AF035751) HypE [Synechococcus PCC7002]
Length = 348
Score = 74.1 bits (179), Expect = 2e-12
Identities = 73/314 (23%), Positives = 143/314 (45%), Gaps = 25/314 (7%)
Query: 30 PGVGIDATAIDFGDYVLVASTD-----PITGAEERIGFYSVHVNANDVATFGAKPKWFLV 84
P DA A+ L +TD P+ IG +++ ND+A GA+P + V
Sbjct: 41 PCTSHDAAALPVSTEKLAFTTDSYVVQPLFFPGGDIGKLAIYGTVNDLAMAGARPLYLSV 100
Query: 85 TILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGHTEVTP-GLKRPIVVGTM-LGEVD-R 141
++ EG E + ++ +++ ++ +G+ IV G T+V G + + T +G ++ R
Sbjct: 101 GFILEEGLPLETLWQVVVSMQQAAQVVGVQIVTGDTKVVERGKGDGLFINTSGIGIIESR 160
Query: 142 DNLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEELRGVFGRDFVERAKAFIEEISIVRE 201
N+ PGD I+++ G G +I+A +RE G+ +E A + + V++
Sbjct: 161 LNIQPKAIAPGDTILVSGDLGRHGIAIMA-QRE----GLAFESPIESDCAPL--VEPVQD 213
Query: 202 AMIAREFATAMHDPTEGGIANGIHEMADINDLGFRIFAEKIIIRDETRKICDFYDLNPLA 261
+ A + D T GG+ ++E+A F++ +I + + R C+ + +P+
Sbjct: 214 LLKAGIEIHCLRDITRGGLGAVLNELAIAAGYQFKVHGNQIPVIEAVRGACEIFGFDPVH 273
Query: 262 LISSGALLISVPRDHAKFLVERLLSYGISASIIGEFLAEK--------KRVIIEN--GIE 311
+ + G + +P A + L Y +A IG + V++EN G+
Sbjct: 274 IANEGRFVAILPEARAAEGLALLQHYNANARQIGLVTTQNCGEQHLAIAPVVVENDLGVT 333
Query: 312 RPLKRPESDELWKV 325
R L+R ++L ++
Sbjct: 334 RILERLSGEQLPRI 347
>pir||F71981 hydrogenase expression/formation protein - Helicobacter pylori
(strain J99) >gi|4154539|gb|AAD05617.1| (AE001443)
HYDROGENASE EXPRESSION/FORMATION PROTEIN [Helicobacter
pylori J99]
Length = 332
Score = 74.1 bits (179), Expect = 2e-12
Identities = 68/277 (24%), Positives = 130/277 (46%), Gaps = 22/277 (7%)
Query: 60 IGFYSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGH 119
IG V +ANDV+ G +P + + ++ EG + I+ I++ + ++
Sbjct: 66 IGKLCVCGSANDVSVQGGEPLYLNMGFILEEGLEIPLLKQILQSIQKELFKANLKLLSLD 125
Query: 120 TEVTP--GLKRPIVVGTMLGEVDRDNLVVPDPKPGDAIIMTKGAGIEGTSIIAHEREEEL 177
T+V P + + + T +G++ + + + G AII++ G S+ A E +L
Sbjct: 126 TKVVPKGSVDKLFINTTCIGKIIKPGISSRHLQQGQAIILSDTIANHGASLFATRHEIKL 185
Query: 178 RGVFGRD----FVERAKAFIEEISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDL 233
+ D + F+ ++ I A+ D T GG+A+ ++E A+ + +
Sbjct: 186 KTNLQSDCQLLYPLLKPLFLSDLKI-----------DALRDATRGGLASVLNEWANSSRV 234
Query: 234 GFRIFAEKIIIRDETRKICDFYDLNPLALISSGALLISVPRDHAKFLVERLLS--YGISA 291
I EKI +++ET+ IC+ L P AL + G ++++ + A +E L S +A
Sbjct: 235 KIVIEEEKIPLKEETKGICEILGLEPYALANEGVFVLALNQKDAPKALEILKSNEKAKNA 294
Query: 292 SII-GEFLAEKKRVIIEN--GIERPLKRPESDELWKV 325
+I G F V+++N G ER L+ PE + L ++
Sbjct: 295 CVIGGVFENPYPSVVLKNAWGFERILEMPEGELLPRI 331
>sp|O27447|THIL_METTH PROBABLE THIAMINE-MONOPHOSPHATE KINASE (THIAMINE-PHOSPHATE KINASE)
>gi|7482853|pir||G69052 thiamin monphosphate kinase -
Methanobacterium thermoautotrophicum (strain Delta H)
>gi|2622507|gb|AAB85873.1| (AE000902) thiamine
monphosphate kinase [Methanobacterium
thermoautotrophicum]
Length = 327
Score = 73.7 bits (178), Expect = 3e-12
Identities = 68/265 (25%), Positives = 128/265 (47%), Gaps = 29/265 (10%)
Query: 15 KIIDGIKPGERVIVGP----GVGIDATAIDFGDYVLVASTD--------PITGAEERIGF 62
K+I I R + G G+G DA ID G+ LV ++D P +G+
Sbjct: 12 KLISRIISRARSVFGSSEVMGLGDDAALIDAGEEFLVLTSDLLLETRHFPDPERPRDMGW 71
Query: 63 YSVHVNANDVATFGAKPKWFLVTILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGHTEV 122
+V VN +D+A G++P+ F++++ +P+ E ++M + E ++GG T
Sbjct: 72 KTVTVNMSDLAAMGSRPEGFILSVALPD-LELEFFDSLMDGVIEACCHYQAPLIGGDTNE 130
Query: 123 TPGLKRPIVVGTMLGEVDRDNLVVPD-PKPGDAIIMT--KGAGIEGTSIIAHEREEELRG 179
+ I+ GT +G ++D +++ +PGD + +T G G GT +I R+ G
Sbjct: 131 ADEI---ILSGTAIGRAEKDMVLMKSGARPGDLVAVTGPLGVGAAGTELILSGRD---TG 184
Query: 180 VFGRDFVERAKAFIEEISIVREAMIARE--FATAMHDPTEGGIANGIHEMADINDLGFRI 237
F + VER+ + ++ + E +I E ++ D T+ G+ + + E+ D + G RI
Sbjct: 185 GF-ENAVERS---LRPVARIEEGLILAESGLVSSATDITD-GLVSELGELMDASGTGMRI 239
Query: 238 FAEKIIIRDETRKICDFYDLNPLAL 262
+ +++ + E +I +PL L
Sbjct: 240 YEDRLPLGREVPEIASILGRDPLEL 264
>pir||D69116 phosphoribosylformylglycinamidine synthase II related protein -
Methanobacterium thermoautotrophicum (strain Delta H)
>gi|2623001|gb|AAB86330.1| (AE000939)
phosphoribosylformylglycinamidine synthase II related
protein [Methanobacterium thermoautotrophicum]
Length = 330
Score = 68.7 bits (165), Expect = 9e-11
Identities = 81/304 (26%), Positives = 137/304 (44%), Gaps = 48/304 (15%)
Query: 31 GVGIDATAIDFGD-YVLVASTDPITGA-----EERIGFYSVHVNANDVATFGAKPKWFLV 84
G G DA+AI GD +L+ + D I G G+ SV VN ND+A G KP +
Sbjct: 44 GYGDDASAIRIGDGKLLLLAADGIWGRLMDADPYWAGYCSVLVNVNDIAAMGGKPVGMVN 103
Query: 85 TILMPEGSSEEDIANIMAEIREVSEAMGIAIVGGHTEVTPGLKRPIVVGTMLGEVDRDNL 144
+ + +S E ++M IR+ ++ G+ +VGGH V P + ++ G D +
Sbjct: 104 VLSI---NSSETCYSVMEGIRDGADKFGVPMVGGH--VHPDTPYDALDVSIAGIASEDAI 158
Query: 145 VVP-DPKPGDAIIMTKGAGIEGT---------SIIAHEREEELRGVFGRDFVERAKAFIE 194
+ D +PGD +I+ G ++G H+ E+++
Sbjct: 159 ITSCDARPGDRVIV--GIDLDGRPHPSFPLNWDTTTHKSPEDVQA--------------- 201
Query: 195 EISIVREAMIAREFATAMHDPTEGGIANGIHEMADINDLGFRIFAEKIIIRDETRKICDF 254
+I I+ E + R TA D + G + + + +++G RI E I R+ + +
Sbjct: 202 QIDIMAE-LAERHLVTAGKDISNPGTLGTLGMLLEASNVGARIEMES-IPRNRSVDWDSW 259
Query: 255 YDLNPLALISSGALLISVPRDHAKFLVERLLSYGISASIIGEFLAEKK---RVIIENGIE 311
+ P SG +L + P ++ VE L S GI+AS GE +A+++ RV + G+
Sbjct: 260 LKMYP----GSGFVLTAAP-ENVNECVELLESVGITASDAGEIIADQRLYLRVDDDEGVL 314
Query: 312 RPLK 315
LK
Sbjct: 315 FDLK 318
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.320 0.141 0.401
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 122101280
Number of Sequences: 2977
Number of extensions: 5131533
Number of successful extensions: 12903
Number of sequences better than 1.0e-10: 35
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 31
Number of HSP's that attempted gapping in prelim test: 12839
Number of HSP's gapped (non-prelim): 37
length of query: 326
length of database: 189,106,746
effective HSP length: 56
effective length of query: 270
effective length of database: 155,591,474
effective search space: 42009697980
effective search space used: 42009697980
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 165 (68.7 bits)