BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB0426 (NusA) DE:transcription termination-antitermination factor NusA, putative (145 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||E75182 probable transcription termination-antitermination f... 292 1e-78 pir||E71031 probable transcription termination-antitermination f... 287 4e-77 sp|P29157|NUSA_THECE NUSA PROTEIN HOMOLOG >gi|99241|pir||S18712 ... 252 1e-66 pir||B69007 probable transcription termination factor nusA - Met... 146 1e-34 gi|11499475 transcription termination-antitermination factor Nus... 145 3e-34 sp|P11523|NUSA_SULAC NUSA PROTEIN HOMOLOG 115 2e-25 pir||A72571 hypothetical protein APE1850 - Aeropyrum pernix (str... 111 4e-24 gb|AAK40563.1| Transcription termination factor nusA homolog, pu... 104 4e-22 pir||S04720 probable transcription termination factor nusA - Sul... 104 7e-22 sp|P14026|NUSA_METVA NUSA PROTEIN HOMOLOG >gi|76322|pir||QQMX2 p... 96 2e-19 sp|Q58447|NUSA_METJA NUSA PROTEIN HOMOLOG >gi|2129311|pir||D6443... 90 1e-17 sp|P15738|NUSA_HALMO NUSA PROTEIN HOMOLOG >gi|81082|pir||S03580 ... 88 4e-17 sp|P15739|NUSA_HALHA NUSA PROTEIN HOMOLOG >gi|81060|pir||S03579 ... 88 5e-17 >pir||E75182 probable transcription termination-antitermination factor nusa PAB0426 - Pyrococcus abyssi (strain Orsay) >gi|5458050|emb|CAB49540.1| (AJ248284) transcription termination-antitermination factor NusA, putative [Pyrococcus abyssi] Length = 145 Score = 292 bits (740), Expect = 1e-78 Identities = 145/145 (100%), Positives = 145/145 (100%) Query: 1 MPLKLNTDQIKYIALFESFTGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVE 60 MPLKLNTDQIKYIALFESFTGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVE Sbjct: 1 MPLKLNTDQIKYIALFESFTGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVE 60 Query: 61 NLLGKEIDLVEHSENPEEFIKNIYKTMGVKVKRVHITEKKDGKKVALLDVNPRDKPKAIG 120 NLLGKEIDLVEHSENPEEFIKNIYKTMGVKVKRVHITEKKDGKKVALLDVNPRDKPKAIG Sbjct: 61 NLLGKEIDLVEHSENPEEFIKNIYKTMGVKVKRVHITEKKDGKKVALLDVNPRDKPKAIG 120 Query: 121 RGGHNINIVRQLMERHHGIEDVIII 145 RGGHNINIVRQLMERHHGIEDVIII Sbjct: 121 RGGHNINIVRQLMERHHGIEDVIII 145 >pir||E71031 probable transcription termination-antitermination factor nusA - Pyrococcus horikoshii >gi|3257970|dbj|BAA30653.1| (AP000006) 145aa long hypothetical transcription termination-antitermination factor nusA [Pyrococcus horikoshii] Length = 145 Score = 287 bits (727), Expect = 4e-77 Identities = 140/145 (96%), Positives = 145/145 (99%) Query: 1 MPLKLNTDQIKYIALFESFTGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVE 60 MPLKLNTDQIKYIALFESFTGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVE Sbjct: 1 MPLKLNTDQIKYIALFESFTGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVE 60 Query: 61 NLLGKEIDLVEHSENPEEFIKNIYKTMGVKVKRVHITEKKDGKKVALLDVNPRDKPKAIG 120 +LLGKEIDL+EHSENPEEFIKNIY+TMGVKVKRVHITEKKDGKKVALLDVNPRDKP+AIG Sbjct: 61 SLLGKEIDLIEHSENPEEFIKNIYRTMGVKVKRVHITEKKDGKKVALLDVNPRDKPRAIG 120 Query: 121 RGGHNINIVRQLMERHHGIEDVIII 145 RGGHNINIVRQLMERHHGIEDVII+ Sbjct: 121 RGGHNINIVRQLMERHHGIEDVIIV 145 >sp|P29157|NUSA_THECE NUSA PROTEIN HOMOLOG >gi|99241|pir||S18712 probable transcription termination factor nusA - Thermococcus celer >gi|48144|emb|CAA47726.1| (X67313) ORF-X [Thermococcus celer] >gi|58411|emb|CAA42848.1| (X60305) orf-x [Thermococcus celer] Length = 145 Score = 252 bits (638), Expect = 1e-66 Identities = 115/145 (79%), Positives = 139/145 (95%) Query: 1 MPLKLNTDQIKYIALFESFTGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVE 60 MPLKLNTDQIK+IALFES TGATVLDCLID RNRLI+VIKKGEMGLALGK+G NV+RV+ Sbjct: 1 MPLKLNTDQIKFIALFESMTGATVLDCLIDDRRNRLIFVIKKGEMGLALGKKGANVKRVQ 60 Query: 61 NLLGKEIDLVEHSENPEEFIKNIYKTMGVKVKRVHITEKKDGKKVALLDVNPRDKPKAIG 120 N++GKEI+++EHSENPEEF++NIYK++GVKVKRVHITEK+DGK+VALLD+ PR+KP+AIG Sbjct: 61 NMIGKEIEVIEHSENPEEFLRNIYKSLGVKVKRVHITEKRDGKRVALLDIGPREKPRAIG 120 Query: 121 RGGHNINIVRQLMERHHGIEDVIII 145 RGG NIN+V++LMERHHGI+DV+II Sbjct: 121 RGGQNINLVKELMERHHGIQDVVII 145 >pir||B69007 probable transcription termination factor nusA - Methanobacterium thermoautotrophicum (strain Delta H) >gi|2622153|gb|AAB85545.1| (AE000876) transcription termination factor NusA [Methanobacterium thermoautotrophicum] Length = 143 Score = 146 bits (364), Expect = 1e-34 Identities = 67/144 (46%), Positives = 102/144 (70%), Gaps = 2/144 (1%) Query: 1 MPLKLNTDQIKYIALFESFTGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVE 60 M +K T++I+YIALFES TGA V DC++D E R+ +++KKG+MGLA+GK+G V +V+ Sbjct: 1 MTIKFTTNEIRYIALFESMTGAMVKDCIVDDENGRVTFLVKKGDMGLAIGKKGSTVAKVQ 60 Query: 61 NLLGKEIDLVEHSENPEEFIKNIYKTMGVKVKRVHITEKKDGKKVALLDVNPRDKPKAIG 120 L K ++++EHS +P EFIKN+ KV+ + I +K++G+K+A ++ +P++K AIG Sbjct: 61 KALDKGVEVIEHSNDPVEFIKNLMAP--AKVRSIRILQKENGEKIATVETDPKNKRIAIG 118 Query: 121 RGGHNINIVRQLMERHHGIEDVII 144 RGG NI R L R H I ++II Sbjct: 119 RGGQNIERARLLARRQHNISNIII 142 >gi|11499475 transcription termination-antitermination factor NusA, putative [Archaeoglobus fulgidus] >gi|7428995|pir||B69486 probable transcription termination factor nusA - Archaeoglobus fulgidus >gi|2648654|gb|AAB89363.1| (AE000972) transcription termination-antitermination factor NusA, putative [Archaeoglobus fulgidus] Length = 139 Score = 145 bits (361), Expect = 3e-34 Identities = 69/144 (47%), Positives = 105/144 (72%), Gaps = 6/144 (4%) Query: 1 MPLKLNTDQIKYIALFESFTGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVE 60 M +KL+ D I+Y+ LFE+ TGA+V DCL+ ++ +++V+++G MG+A+GK GINV R Sbjct: 1 MGVKLSADGIRYLTLFENLTGASVKDCLVYDDK--VVFVVRRGHMGVAIGKGGINVERAR 58 Query: 61 NLLGKEIDLVEHSENPEEFIKNIYKTMGVKVKRVHITEKKDGKKVALLDVNPRDKPKAIG 120 L+GK I+++EHSE+P EFI NI+K + V VK + +K+G K+A++ V+P K IG Sbjct: 59 ELIGKRIEIIEHSEDPAEFIANIFKPIKVNVKLM----EKEGSKIAVVSVSPDLKGIVIG 114 Query: 121 RGGHNINIVRQLMERHHGIEDVII 144 +GG NIN ++L +RHH IEDVI+ Sbjct: 115 KGGKNINKAKELAKRHHDIEDVIV 138 >sp|P11523|NUSA_SULAC NUSA PROTEIN HOMOLOG Length = 143 Score = 115 bits (286), Expect = 2e-25 Identities = 51/143 (35%), Positives = 91/143 (62%), Gaps = 3/143 (2%) Query: 3 LKLNTDQIKYIALFESFTGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVENL 62 +KL ++++KY++LF+ TG T DC+ID R+I +I MG+A+GK G+NVR++E L Sbjct: 4 IKLTSEELKYMSLFQDITGVTARDCIIDDRNKRIILLINPESMGVAIGKNGLNVRKLEKL 63 Query: 63 LGKEIDLVEHSENPEEFIKNIYKTMGVKVKRVHITEKKDGKKVALLDVNPRDKPKAIGRG 122 + K +++V + EN E+ +KN+ VK +V + + KK + V P+DK AIG+ Sbjct: 64 INKSVEIVGYQENLEDLVKNLMSPARVKTIKV---VQSNSKKTVYITVEPQDKGIAIGKN 120 Query: 123 GHNINIVRQLMERHHGIEDVIII 145 G N+ + +++R+ I+ V+++ Sbjct: 121 GRNVERAKLILKRYLDIDSVVVV 143 >pir||A72571 hypothetical protein APE1850 - Aeropyrum pernix (strain K1) >gi|5105541|dbj|BAA80854.1| (AP000062) 144aa long hypothetical protein [Aeropyrum pernix] Length = 144 Score = 111 bits (275), Expect = 4e-24 Identities = 50/141 (35%), Positives = 94/141 (66%), Gaps = 3/141 (2%) Query: 4 KLNTDQIKYIALFESFTGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVENLL 63 ++ ++++YI++F S TG T C++D+E NRLI+++ +GE G A+G+ G ++ + L Sbjct: 6 RITLEELRYISVFHSITGVTAYRCIVDEENNRLIFLVSEGEAGRAIGRGGRLIKLLREAL 65 Query: 64 GKEIDLVEHSENPEEFIKNIYKTMGVKVKRVHITEKKDGKKVALLDVNPRDKPKAIGRGG 123 GK I++VE+S + E +KN++ GVK++ +++ E ++G K ++ V+ DK AIG+GG Sbjct: 66 GKNIEVVEYSSDLERIVKNLFP--GVKIESINVRE-RNGVKQVVIKVSEDDKGAAIGKGG 122 Query: 124 HNINIVRQLMERHHGIEDVII 144 N+ R ++ + G+E V+I Sbjct: 123 KNVKRARLVLSKLFGVEKVVI 143 >gb|AAK40563.1| Transcription termination factor nusA homolog, putative (nusA) [Sulfolobus solfataricus] Length = 124 Score = 104 bits (258), Expect = 4e-22 Identities = 48/126 (38%), Positives = 86/126 (68%), Gaps = 3/126 (2%) Query: 20 TGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVENLLGKEIDLVEHSENPEEF 79 T A V DC+ID E NR+I+++ +MG+A+GK GINV++++ ++GK+I+LV +S+N EE Sbjct: 2 TRANVRDCIIDNENNRIIFLVDSKDMGVAIGKSGINVKKLKKIIGKDIELVAYSDNLEEL 61 Query: 80 IKNIYKTMGVKVKRVHITEKKDGKKVALLDVNPRDKPKAIGRGGHNINIVRQLMERHHGI 139 +KN+ V+ +V T KK ++++P+DK AIG+ G N+ + +++R+ I Sbjct: 62 VKNLMSPARVRSVKVVNT---SSKKSVYINIDPQDKGLAIGKNGRNVARAKLILKRYMDI 118 Query: 140 EDVIII 145 ++V+I+ Sbjct: 119 DNVVIV 124 >pir||S04720 probable transcription termination factor nusA - Sulfolobus acidocaldarius >gi|46673|emb|CAA32928.1| (X14818) ORF 130 (AA 1-130) [Sulfolobus acidocaldarius] Length = 130 Score = 104 bits (256), Expect = 7e-22 Identities = 47/133 (35%), Positives = 82/133 (61%), Gaps = 3/133 (2%) Query: 13 IALFESFTGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVENLLGKEIDLVEH 72 ++LF+ TG T DC+ID R+I +I MG+A+GK G+NVR++E L+ K +++V + Sbjct: 1 MSLFQDITGVTARDCIIDDRNKRIILLINPESMGVAIGKNGLNVRKLEKLINKSVEIVGY 60 Query: 73 SENPEEFIKNIYKTMGVKVKRVHITEKKDGKKVALLDVNPRDKPKAIGRGGHNINIVRQL 132 EN E+ +KN+ VK +V + + KK + V P+DK AIG+ G N+ + + Sbjct: 61 QENLEDLVKNLMSPARVKTIKV---VQSNSKKTVYITVEPQDKGIAIGKNGRNVERAKLI 117 Query: 133 MERHHGIEDVIII 145 ++R+ I+ V+++ Sbjct: 118 LKRYLDIDSVVVV 130 >sp|P14026|NUSA_METVA NUSA PROTEIN HOMOLOG >gi|76322|pir||QQMX2 probable transcription termination factor nusA - Methanococcus vannielii >gi|44783|emb|CAA34088.1| (X15970) ORF 2 protein (AA 1-173) [Methanococcus vannielii] Length = 173 Score = 96.0 bits (235), Expect = 2e-19 Identities = 54/146 (36%), Positives = 90/146 (60%), Gaps = 7/146 (4%) Query: 1 MPLKLNTDQIKYIALFESFTGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVE 60 M +KLNT+ I I+LFE TGA V+D D E+ +++V+K+G++G A+GK G NV+ Sbjct: 1 MRIKLNTEDIMRISLFEKMTGANVIDSTSDDEK--IVFVVKEGDIGAAIGKGGENVKNAT 58 Query: 61 NLLGKEIDLVEHSENPEEFIKNIYKTMGVKVKRVHITEKKDGKK-VALLDVNPRDKPKAI 119 + GK+IDL+E+SE+ ++FIKNI+ ++ + KK G V + V+PR + I Sbjct: 59 DKFGKKIDLIEYSEDLKQFIKNIF----APIELEDVWVKKFGNDLVVYVRVHPRLRRAII 114 Query: 120 GRGGHNINIVRQLMERHHGIEDVIII 145 G G NI+ + R G++++ ++ Sbjct: 115 GDKGKNIDRAVDIAGRLAGVKNIKVV 140 >sp|Q58447|NUSA_METJA NUSA PROTEIN HOMOLOG >gi|2129311|pir||D64430 probable transcription termination factor nusA - Methanococcus jannaschii >gi|1591699|gb|AAB99049.1| (U67547) putative transcription termination-antitermination factor (NusA) [Methanococcus jannaschii] Length = 183 Score = 89.7 bits (219), Expect = 1e-17 Identities = 44/144 (30%), Positives = 86/144 (59%), Gaps = 7/144 (4%) Query: 3 LKLNTDQIKYIALFESFTGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVENL 62 ++L T++I I FE +LDC+++ ER + +++K+G++G A+GK G NV+ E Sbjct: 4 VRLTTEEIMKIGFFEKIANVPILDCVLNDER--VAFIVKEGDVGAAIGKGGENVKTAEEK 61 Query: 63 LGKEIDLVEHSENPEEFIKNIYKTMGVKVKRVHITEKKDGKK-VALLDVNPRDKPKAIGR 121 GK++D++E+S++ +FI+NI+ ++ + K+ GK VA + +NP+ + G Sbjct: 62 FGKKVDIIEYSDDWRKFIRNIF----APIQLDDVWVKRVGKDVVAFIKINPKVRRAVFGE 117 Query: 122 GGHNINIVRQLMERHHGIEDVIII 145 G N+ ++++RH I + +I Sbjct: 118 KGKNLERALKILKRHTKITKIKVI 141 >sp|P15738|NUSA_HALMO NUSA PROTEIN HOMOLOG >gi|81082|pir||S03580 probable transcription termination factor nusA - Halococcus morrhuae >gi|43623|emb|CAA40433.1| (X57145) ORF 139 [Halococcus morrhuae] Length = 139 Score = 88.2 bits (215), Expect = 4e-17 Identities = 50/142 (35%), Positives = 79/142 (55%), Gaps = 6/142 (4%) Query: 1 MPLKLNTDQIKYIALFESFTGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVE 60 M + L+ +++IALFE TGA+ +DCL+D +R + +++ GEM A+G G V +VE Sbjct: 1 MRVTLSDTALRHIALFEDVTGASAVDCLVDDDR--VCFLVATGEMSDAIGPDGTTVEQVE 58 Query: 61 NLLGKEIDLVEHSENPEEFIKNIYKTMGVKVKRVHITEKKDGKKVALLDVNPRDKPKAIG 120 L K + LVE+++ E F+ N V ++T ++ VA +V+ D AIG Sbjct: 59 ERLDKRVSLVENADTAELFVANALAPAVVH----NVTISENASTVAYAEVDRADTGVAIG 114 Query: 121 RGGHNINIVRQLMERHHGIEDV 142 R G I R+L ER I+D+ Sbjct: 115 RDGETIETARRLAERQFDIDDI 136 >sp|P15739|NUSA_HALHA NUSA PROTEIN HOMOLOG >gi|81060|pir||S03579 probable transcription termination factor nusA - Halobacterium salinarum >gi|43544|emb|CAA40428.1| (X57144) ORF 139 [Halobacterium halobium] >gi|10582041|gb|AAG20690.1| (AE005138) transcription termination-antitermination factor; NusA [Halobacterium sp. NRC-1] Length = 139 Score = 87.8 bits (214), Expect = 5e-17 Identities = 51/142 (35%), Positives = 78/142 (54%), Gaps = 6/142 (4%) Query: 1 MPLKLNTDQIKYIALFESFTGATVLDCLIDQERNRLIYVIKKGEMGLALGKRGINVRRVE 60 M ++L+ + + IA+FE+ T AT +DCL D +R ++YV+ GEMG A+G G V +E Sbjct: 1 MTIRLSDEARQLIAVFETETDATAVDCLPDDDR--VVYVVTAGEMGAAIGDGGSRVDALE 58 Query: 61 NLLGKEIDLVEHSENPEEFIKNIYKTMGVKVKRVHITEKKDGKKVALLDVNPRDKPKAIG 120 LG+ + LVE + E F+ N V ++T ++ VA +V DK AIG Sbjct: 59 ATLGRSVVLVEDAPTAEGFVANALSPAAV----YNVTVSENDTTVAYAEVAHEDKGVAIG 114 Query: 121 RGGHNINIVRQLMERHHGIEDV 142 G NI ++L RH I+D+ Sbjct: 115 ADGTNIETAKELAARHFDIDDI 136 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.320 0.142 0.399 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 52429923 Number of Sequences: 2977 Number of extensions: 2079875 Number of successful extensions: 6035 Number of sequences better than 1.0e-10: 13 Number of HSP's better than 0.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 6002 Number of HSP's gapped (non-prelim): 13 length of query: 145 length of database: 189,106,746 effective HSP length: 53 effective length of query: 92 effective length of database: 157,386,935 effective search space: 14479598020 effective search space used: 14479598020 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.8 bits) S2: 161 (67.1 bits)