BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0511 (dys1) DE:deoxyhypusine synthase (dys1)
(335 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||C75119 deoxyhypusine synthase (EC 1.1.1.249) dys1 PAB0511 [... 679 0.0
sp|O50105|DHYS_PYRHO PROBABLE DEOXYHYPUSINE SYNTHASE (DHS) >gi|7... 622 e-177
sp|Q58224|DHYS_METJA PROBABLE DEOXYHYPUSINE SYNTHASE (DHS) 517 e-146
pir||F64401 deoxyhypusine synthase (EC 1.1.1.249) MJ0814 [simila... 517 e-146
emb|CAB52544.1| (AJ010120) homospermidine synthase [Senecio vulg... 289 2e-77
emb|CAB65462.1| (AJ238623) homospermidine synthase [Senecio vern... 285 6e-76
emb|CAB66389.1| (AJ251500) homospermidine synthase [Senecio vulg... 285 6e-76
sp|O94337|DHYS_SCHPO PROBABLE DEOXYHYPUSINE SYNTHASE (DHS) >gi|6... 282 4e-75
emb|CAB62400.1| (AJ242017) deoxyhypusine synthase [Nicotiana tab... 282 5e-75
emb|CAB65461.1| (AJ238622) deoxyhypusine synthase [Senecio verna... 281 9e-75
sp|Q9VSF4|DHYS_DROME PROBABLE DEOXYHYPUSINE SYNTHASE (DHS) >gi|7... 277 1e-73
dbj|BAB10797.1| (AB017060) deoxyhypusine synthase [Arabidopsis t... 277 1e-73
pir||T39340 deoxyhypusine synthase (EC 1.1.1.249) SPBC1271.04c [... 276 2e-73
gb|AAG19745.1| (AE005060) deoxyhypusine synthase; Dhs [Halobacte... 276 3e-73
pdb|1DHS| Crystal Structure Of The Nad Complex Of Human Deoxyh... 272 4e-72
sp|Q9XXJ0|DHYS_CAEEL PROBABLE DEOXYHYPUSINE SYNTHASE (DHS) >gi|7... 266 2e-70
pir||T51022 deoxyhypusine synthase (EC 1.1.1.249) B7F21.30 [simi... 266 2e-70
gi|4503325 deoxyhypusine synthase isoform a [Homo sapiens] >gi|1... 266 3e-70
pir||S68692 deoxyhypusine synthase (EC 1.1.1.249) [validated] - ... 266 3e-70
sp|P49365|DHYS_NEUCR DEOXYHYPUSINE SYNTHASE (DHS) >gi|11280362|p... 265 4e-70
gi|6321859 Deoxyhypusine synthase; Dys1p [Saccharomyces cerevisi... 262 4e-69
pir||S71138 deoxyhypusine synthase long form - human >gi|1335786... 257 2e-67
emb|CAB95376.1| (AL359782) possible deoxyhypusine synthase [Tryp... 210 3e-53
emb|CAC11500.1| (AL445064) deoxyhypusine synthase related protei... 203 2e-51
gi|7108338 deoxyhypusine synthase isoform a [Homo sapiens] >gi|1... 193 2e-48
gb|AAB02175.1| (U26266) deoxyhypusine synthase [Homo sapiens] 192 5e-48
gi|7108340 deoxyhypusine synthase isoform a [Homo sapiens] >gi|1... 176 2e-43
sp|Q9YE72|DHYS_AERPE PROBABLE DEOXYHYPUSINE SYNTHASE (DHS) >gi|7... 175 7e-43
gb|AAK41241.1| Deoxyhypusine synthase [Sulfolobus solfataricus] 170 2e-41
gi|11499881 deoxyhypusine synthase (dys1-2) [Archaeoglobus fulgi... 159 6e-38
gb|AAG01866.1|AF290977_1 (AF290977) deoxyhypusine synthase [Plas... 157 2e-37
sp|O26230|DHYS_METTH PROBABLE DEOXYHYPUSINE SYNTHASE (DHS) >gi|7... 144 1e-33
gi|11499777 deoxyhypusine synthase (dys1-1) [Archaeoglobus fulgi... 133 2e-30
>pir||C75119 deoxyhypusine synthase (EC 1.1.1.249) dys1 PAB0511 [similarity] -
Pyrococcus abyssi (strain Orsay)
>gi|5458179|emb|CAB49668.1| (AJ248285) deoxyhypusine
synthase (dys1) [Pyrococcus abyssi]
Length = 335
Score = 679 bits (1732), Expect = 0.0
Identities = 335/335 (100%), Positives = 335/335 (100%)
Query: 1 MKAKDIVLKKSEEIEGLAIEGPWLDEVESLEGVISYYEKIGFQATHLGKAVKIWRKVEEK 60
MKAKDIVLKKSEEIEGLAIEGPWLDEVESLEGVISYYEKIGFQATHLGKAVKIWRKVEEK
Sbjct: 1 MKAKDIVLKKSEEIEGLAIEGPWLDEVESLEGVISYYEKIGFQATHLGKAVKIWRKVEEK 60
Query: 61 RKGGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGGIEEDFIKTLKPFILGD 120
RKGGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGGIEEDFIKTLKPFILGD
Sbjct: 61 RKGGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGGIEEDFIKTLKPFILGD 120
Query: 121 WEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIERKLKRPLTASEFIYEM 180
WEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIERKLKRPLTASEFIYEM
Sbjct: 121 WEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIERKLKRPLTASEFIYEM 180
Query: 181 GRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFFKEERHDSKLVIDIAND 240
GRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFFKEERHDSKLVIDIAND
Sbjct: 181 GRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFFKEERHDSKLVIDIAND 240
Query: 241 IVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYISTAVPWDGSLSGAPPS 300
IVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYISTAVPWDGSLSGAPPS
Sbjct: 241 IVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYISTAVPWDGSLSGAPPS 300
Query: 301 EGVSWGKIKAKADYVEIWADATLVFPILVWMVMKA 335
EGVSWGKIKAKADYVEIWADATLVFPILVWMVMKA
Sbjct: 301 EGVSWGKIKAKADYVEIWADATLVFPILVWMVMKA 335
>sp|O50105|DHYS_PYRHO PROBABLE DEOXYHYPUSINE SYNTHASE (DHS) >gi|7451177|pir||G71012
deoxyhypusine synthase (EC 1.1.1.249) PH1397
[similarity] - Pyrococcus horikoshii
>gi|3257820|dbj|BAA30503.1| (AP000006) 342aa long
hypothetical deoxyhypusine synthase [Pyrococcus
horikoshii]
Length = 342
Score = 622 bits (1586), Expect = e-177
Identities = 293/335 (87%), Positives = 322/335 (95%)
Query: 1 MKAKDIVLKKSEEIEGLAIEGPWLDEVESLEGVISYYEKIGFQATHLGKAVKIWRKVEEK 60
MKAKDIVLKKSE+IEG+ ++GPWLD+ +SLE V+SYY +IGFQATHLG+A++IWRKVEEK
Sbjct: 1 MKAKDIVLKKSEKIEGVEVKGPWLDDAQSLEEVVSYYYRIGFQATHLGRAIEIWRKVEEK 60
Query: 61 RKGGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGGIEEDFIKTLKPFILGD 120
R+ GEE+RVFLGYTSNI+SSGLREIIAWLVKE+KVDVIVTTAGG+EEDFIK+LKPFILGD
Sbjct: 61 RERGEEIRVFLGYTSNIISSGLREIIAWLVKEKKVDVIVTTAGGVEEDFIKSLKPFILGD 120
Query: 121 WEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIERKLKRPLTASEFIYEM 180
WEV+DAELR+KG+NRIGNIFVPNDRYIEFEKYM+PFFER+L IE KL RPLTASEFIYEM
Sbjct: 121 WEVDDAELRKKGVNRIGNIFVPNDRYIEFEKYMIPFFERVLKIEEKLSRPLTASEFIYEM 180
Query: 181 GRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFFKEERHDSKLVIDIAND 240
GRYMDE LGKEKEKS+IYWAYK ++PIFCPAITDGSIGDMLYFFKEER DS+L+IDIAND
Sbjct: 181 GRYMDEKLGKEKEKSVIYWAYKNNIPIFCPAITDGSIGDMLYFFKEERRDSRLIIDIAND 240
Query: 241 IVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYISTAVPWDGSLSGAPPS 300
IVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYISTAVPWDGSLSGAPP
Sbjct: 241 IVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYISTAVPWDGSLSGAPPR 300
Query: 301 EGVSWGKIKAKADYVEIWADATLVFPILVWMVMKA 335
EGVSWGKIKAKADYVE+W DATL+FPILVWMVMKA
Sbjct: 301 EGVSWGKIKAKADYVEVWGDATLIFPILVWMVMKA 335
>sp|Q58224|DHYS_METJA PROBABLE DEOXYHYPUSINE SYNTHASE (DHS)
Length = 351
Score = 517 bits (1318), Expect = e-146
Identities = 245/333 (73%), Positives = 294/333 (87%), Gaps = 2/333 (0%)
Query: 4 KDIVLKKSEEIEGLAIEGPWLDEVESLEGVIS-YYEKIGFQATHLGKAVKIWRKVEEKRK 62
KDIVLK+SE+IEG+AIEGPWL++ SLE +I YY KIGFQA+H+GKA+KIW+ +EEKRK
Sbjct: 19 KDIVLKESEDIEGIAIEGPWLEDDISLEEIIKKYYLKIGFQASHIGKAIKIWKHIEEKRK 78
Query: 63 GGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGGIEEDFIKTLKPFILGDWE 122
G+E+ VF GYTSNIVSSGLREIIA+LVK +K+D+IVTTAGG+EEDFIK LKPFILGDWE
Sbjct: 79 KGDEITVFFGYTSNIVSSGLREIIAYLVKHKKIDIIVTTAGGVEEDFIKCLKPFILGDWE 138
Query: 123 VNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIERKLKRPLTASEFIYEMGR 182
V+ LREKGINRIGNIFVPNDRYI FE+YM+ FFE IL+++R+ + +TASEF Y++G
Sbjct: 139 VDGKMLREKGINRIGNIFVPNDRYIAFEEYMMEFFEEILNLQRETGKIITASEFCYKLGE 198
Query: 183 YMDEVL-GKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFFKEERHDSKLVIDIANDI 241
+MD+ L KEKEKSI+YWAYK ++PIFCPAITDGSIGDMLYFFK+ D +L ID+ANDI
Sbjct: 199 FMDKKLKSKEKEKSILYWAYKNNIPIFCPAITDGSIGDMLYFFKKYNKDEELKIDVANDI 258
Query: 242 VKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYISTAVPWDGSLSGAPPSE 301
VKLN++AI +KETA I+LGGSLPKH+IINANLFR GTDYAIY++TA+PWDGSLSGAPP E
Sbjct: 259 VKLNDIAINSKETACIVLGGSLPKHSIINANLFREGTDYAIYVTTALPWDGSLSGAPPEE 318
Query: 302 GVSWGKIKAKADYVEIWADATLVFPILVWMVMK 334
GVSWGKI AKADYVEIW DAT++FP+LV+ VMK
Sbjct: 319 GVSWGKIGAKADYVEIWGDATIIFPLLVYCVMK 351
>pir||F64401 deoxyhypusine synthase (EC 1.1.1.249) MJ0814 [similarity] -
Methanococcus jannaschii >gi|1591503|gb|AAB98813.1|
(U67525) deoxyhypusine synthase (dys1) [Methanococcus
jannaschii]
Length = 370
Score = 517 bits (1318), Expect = e-146
Identities = 245/333 (73%), Positives = 294/333 (87%), Gaps = 2/333 (0%)
Query: 4 KDIVLKKSEEIEGLAIEGPWLDEVESLEGVIS-YYEKIGFQATHLGKAVKIWRKVEEKRK 62
KDIVLK+SE+IEG+AIEGPWL++ SLE +I YY KIGFQA+H+GKA+KIW+ +EEKRK
Sbjct: 38 KDIVLKESEDIEGIAIEGPWLEDDISLEEIIKKYYLKIGFQASHIGKAIKIWKHIEEKRK 97
Query: 63 GGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGGIEEDFIKTLKPFILGDWE 122
G+E+ VF GYTSNIVSSGLREIIA+LVK +K+D+IVTTAGG+EEDFIK LKPFILGDWE
Sbjct: 98 KGDEITVFFGYTSNIVSSGLREIIAYLVKHKKIDIIVTTAGGVEEDFIKCLKPFILGDWE 157
Query: 123 VNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIERKLKRPLTASEFIYEMGR 182
V+ LREKGINRIGNIFVPNDRYI FE+YM+ FFE IL+++R+ + +TASEF Y++G
Sbjct: 158 VDGKMLREKGINRIGNIFVPNDRYIAFEEYMMEFFEEILNLQRETGKIITASEFCYKLGE 217
Query: 183 YMDEVL-GKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFFKEERHDSKLVIDIANDI 241
+MD+ L KEKEKSI+YWAYK ++PIFCPAITDGSIGDMLYFFK+ D +L ID+ANDI
Sbjct: 218 FMDKKLKSKEKEKSILYWAYKNNIPIFCPAITDGSIGDMLYFFKKYNKDEELKIDVANDI 277
Query: 242 VKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYISTAVPWDGSLSGAPPSE 301
VKLN++AI +KETA I+LGGSLPKH+IINANLFR GTDYAIY++TA+PWDGSLSGAPP E
Sbjct: 278 VKLNDIAINSKETACIVLGGSLPKHSIINANLFREGTDYAIYVTTALPWDGSLSGAPPEE 337
Query: 302 GVSWGKIKAKADYVEIWADATLVFPILVWMVMK 334
GVSWGKI AKADYVEIW DAT++FP+LV+ VMK
Sbjct: 338 GVSWGKIGAKADYVEIWGDATIIFPLLVYCVMK 370
>emb|CAB52544.1| (AJ010120) homospermidine synthase [Senecio vulgaris]
Length = 384
Score = 289 bits (733), Expect = 2e-77
Identities = 160/353 (45%), Positives = 227/353 (63%), Gaps = 37/353 (10%)
Query: 3 AKDIVLKKSEEIEGLAIE------GPWLDEVESLEGVISYYEKIGFQATHLGKAVKI--- 53
A+ V K+SE +EG + +D + L+ ++S GFQA++LG A+ I
Sbjct: 26 ARSNVFKESESLEGTCAKIGGYDFNNGIDHSKLLKSMVS----TGFQASNLGDAMIITNQ 81
Query: 54 ---WRKV---------EEKRKGGEEVR--VFLGYTSNIVSSGLREIIAWLVKERKVDVIV 99
WR EE+RK E V+ +FLG+TSN++SSG+RE I +L + R VDV+V
Sbjct: 82 MLDWRLSHDEVPENCSEEERKNRESVKCKIFLGFTSNLISSGVRETICYLTQHRMVDVLV 141
Query: 100 TTAGGIEEDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFER 159
TT GGIEEDFIK L G++ + A+LR KG+NRIGN+ VPND YI+FE +++P F++
Sbjct: 142 TTTGGIEEDFIKCLASTYKGEFSLPGADLRSKGLNRIGNLIVPNDNYIKFEDWIIPIFDQ 201
Query: 160 ILDIERKLKRPL-TASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIG 218
+L IE+K + L T S+ I +G+ ++ E S +YWAYK ++P+FCP+ITDGS+G
Sbjct: 202 ML-IEQKTQNVLWTPSKMIARLGKEIN------NETSYLYWAYKNNIPVFCPSITDGSVG 254
Query: 219 DMLYFFKEERHDSKLVIDIANDIVKLNNLAITA--KETASIILGGSLPKHAIINANLFRG 276
DMLYF + LV+DI D++ ++N A+ A ++T IILGG LPKH I NAN+ R
Sbjct: 255 DMLYFHSVSNPGAGLVVDIVQDVIAMDNEAVHASPQKTGIIILGGGLPKHHICNANMMRN 314
Query: 277 GTDYAIYISTAVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPILV 329
G D+A++I+TA +DGS SGA P E VSWGKI + VE+ DAT+ FP+LV
Sbjct: 315 GADFAVFINTAQEYDGSDSGARPDEAVSWGKISSTGKAVEVHCDATIAFPLLV 367
>emb|CAB65462.1| (AJ238623) homospermidine synthase [Senecio vernalis]
Length = 370
Score = 285 bits (721), Expect = 6e-76
Identities = 159/353 (45%), Positives = 224/353 (63%), Gaps = 37/353 (10%)
Query: 3 AKDIVLKKSEEIEGLAIE------GPWLDEVESLEGVISYYEKIGFQATHLGKAVKI--- 53
A+ V K+SE +EG + +D + L+ ++S GFQA++LG A+ I
Sbjct: 12 ARSNVFKESESLEGTCAKIGGYDFNNGIDHSKLLKSMVS----TGFQASNLGDAMIITNQ 67
Query: 54 ---WRKV---------EEKRKGGEEVR--VFLGYTSNIVSSGLREIIAWLVKERKVDVIV 99
WR EE++K E V+ +FLG+TSN++SSG+RE I +L + R VDV+V
Sbjct: 68 MLDWRLSHDEVPENCSEEEKKNRESVKCKIFLGFTSNLISSGVRETICYLTQHRMVDVLV 127
Query: 100 TTAGGIEEDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFER 159
TT GGIEEDFIK L G + + A+LR KG+NRIGN+ VPND YI+FE +++P F++
Sbjct: 128 TTTGGIEEDFIKCLASTYKGKFSLPGADLRSKGLNRIGNLIVPNDNYIKFEDWIIPIFDQ 187
Query: 160 ILDIERKLKRPL-TASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIG 218
+L IE+K + L T S I +G+ ++ E S +YWAYK ++P+FCP+ITDGSIG
Sbjct: 188 ML-IEQKTQNVLWTPSRMIARLGKEIN------NETSYLYWAYKNNIPVFCPSITDGSIG 240
Query: 219 DMLYFFKEERHDSKLVIDIANDIVKLNNLAITA--KETASIILGGSLPKHAIINANLFRG 276
DMLYF LV+DI D++ ++N A+ A ++T IILGG LPKH I NAN+ R
Sbjct: 241 DMLYFHSVSNPGPGLVVDIVQDVIAMDNEAVHASPQKTGIIILGGGLPKHHICNANMMRN 300
Query: 277 GTDYAIYISTAVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPILV 329
G D+A++I+TA +DGS SGA P E VSWGKI + V++ DAT+ FP+LV
Sbjct: 301 GADFAVFINTAQEYDGSDSGARPDEAVSWGKISSTGKAVKVHCDATIAFPLLV 353
>emb|CAB66389.1| (AJ251500) homospermidine synthase [Senecio vulgaris]
Length = 370
Score = 285 bits (721), Expect = 6e-76
Identities = 159/353 (45%), Positives = 224/353 (63%), Gaps = 37/353 (10%)
Query: 3 AKDIVLKKSEEIEGLAIE------GPWLDEVESLEGVISYYEKIGFQATHLGKAVKI--- 53
A+ V K+SE +EG + +D + L+ ++S GFQA++LG A+ I
Sbjct: 12 ARSNVFKESESLEGTCAKIGGYDFNNGIDHSKLLKSMVS----TGFQASNLGDAMIITNQ 67
Query: 54 ---WRKV---------EEKRKGGEEVR--VFLGYTSNIVSSGLREIIAWLVKERKVDVIV 99
WR EE++K E V+ +FLG+TSN++SSG+RE I +L + R VDV+V
Sbjct: 68 MLEWRLSHDEVPENCSEEEKKNRESVKCKIFLGFTSNLISSGVRETICYLTQHRMVDVLV 127
Query: 100 TTAGGIEEDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFER 159
TT GGIEEDFIK L G + + A+LR KG+NRIGN+ VPND YI+FE +++P F++
Sbjct: 128 TTTGGIEEDFIKCLASTYKGKFSLPGADLRSKGLNRIGNLIVPNDNYIKFEDWIIPIFDQ 187
Query: 160 ILDIERKLKRPL-TASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIG 218
+L IE+K + L T S I +G+ ++ E S +YWAYK ++P+FCP+ITDGSIG
Sbjct: 188 ML-IEQKTQNVLWTPSRMIARLGKEIN------NETSYLYWAYKNNIPVFCPSITDGSIG 240
Query: 219 DMLYFFKEERHDSKLVIDIANDIVKLNNLAITA--KETASIILGGSLPKHAIINANLFRG 276
DMLYF LV+DI D++ ++N A+ A ++T IILGG LPKH I NAN+ R
Sbjct: 241 DMLYFHSVSNPGPGLVVDIVQDVIAMDNEAVHASPQKTGIIILGGGLPKHHICNANMMRN 300
Query: 277 GTDYAIYISTAVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPILV 329
G D+A++I+TA +DGS SGA P E VSWGKI + V++ DAT+ FP+LV
Sbjct: 301 GADFAVFINTAQEYDGSDSGARPDEAVSWGKISSTGKAVKVHCDATIAFPLLV 353
>sp|O94337|DHYS_SCHPO PROBABLE DEOXYHYPUSINE SYNTHASE (DHS) >gi|6912225|emb|CAA22194.2|
(AL034353) deoxyhypusine synthase [Schizosaccharomyces
pombe]
Length = 350
Score = 282 bits (714), Expect = 4e-75
Identities = 147/345 (42%), Positives = 217/345 (62%), Gaps = 24/345 (6%)
Query: 3 AKDIVLKKSEEIEGLAIE--GPWLDEVE----------SLEGVISYYEKIGFQATHLGKA 50
AKD V S + L E GP ++ + ++E ++ Y KIGFQAT+L A
Sbjct: 6 AKDAVFVASSKPSDLTTEVVGPDFNKYDERKKAGGPGITVEELVESYGKIGFQATNLHDA 65
Query: 51 VKI------WRKVEEKRKGGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGG 104
V I WR + + +FLGYTSN++SSG+RE++ +LV+ + VDVIVTTAGG
Sbjct: 66 VTIINEMRNWRDPNPPEEKSDRATIFLGYTSNLISSGVREVLRYLVQHKCVDVIVTTAGG 125
Query: 105 IEEDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIE 164
+EED IK L P +GD+ ++ LR KG+NRIGN+ VPND Y FE+++ P ++++ +
Sbjct: 126 VEEDIIKCLGPTYVGDFHLDGKNLRAKGLNRIGNLIVPNDNYCRFEEWIFPILNKMVEEQ 185
Query: 165 RKLKRPLTASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFF 224
L T S FI +G+ +++ E S++YWAYK ++PI+ PA+TDGSIGDMLYF
Sbjct: 186 ETLGTHWTPSSFIRRLGKEIND------ESSVLYWAYKNNIPIYSPALTDGSIGDMLYFH 239
Query: 225 KEERHDSKLVIDIANDIVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYI 284
+ LV+DI DI +N A+ A ++ IILGG + KH I NANL R G ++++YI
Sbjct: 240 TYKATPRPLVLDIVADIRNMNTHAVRANKSGIIILGGGVVKHHICNANLMRNGAEWSVYI 299
Query: 285 STAVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPILV 329
++A +DGS +GA P E VSWGKI+ A V+++ + +L+FP++V
Sbjct: 300 NSANEFDGSDAGARPDEAVSWGKIRGDAKKVKVYGEVSLLFPLIV 344
>emb|CAB62400.1| (AJ242017) deoxyhypusine synthase [Nicotiana tabacum]
Length = 379
Score = 282 bits (713), Expect = 5e-75
Identities = 159/356 (44%), Positives = 220/356 (61%), Gaps = 36/356 (10%)
Query: 4 KDIVLKKSEEIEGLA--IEGPWLDEVESLEGVISYYEKIGFQATHLGKAVKI------WR 55
+ IV K+SE +EG A IEG ++ + + GFQA +LG A++I WR
Sbjct: 12 RSIVFKESENLEGSATKIEGYDFNKGVNYAELFKSMASTGFQAANLGDAIQIVNQMLDWR 71
Query: 56 KVEEK--RKGGEEVR-----------VFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTA 102
E+ EE R +FLG+TSN+VSSG+R+ I +LV+ R VDV+VTTA
Sbjct: 72 LSHEQPMEDCSEEERDVAYRESVTCKIFLGFTSNLVSSGVRDTIRYLVQHRMVDVVVTTA 131
Query: 103 GGIEEDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILD 162
GGIEED IK L P GD+ + A LR KG+NRIGN+ VPND Y +FE +++P F+++ +
Sbjct: 132 GGIEEDLIKCLAPTYKGDFSLPGAVLRSKGLNRIGNLLVPNDNYCKFENWIIPIFDQMYE 191
Query: 163 IERKLKRPLTASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLY 222
+ K K T S+ I + + +++ E S +YWAYK +P+FCP +TDGS+GDMLY
Sbjct: 192 EQIKEKVLWTPSKVIARLAKEIND------ETSYLYWAYKNRIPVFCPGLTDGSLGDMLY 245
Query: 223 FFKEERHDSK-------LVIDIANDIVKLNNLAITA--KETASIILGGSLPKHAIINANL 273
F ++ D L+IDI DI +N+ A+ A ++T IILGG LPKH + NAN+
Sbjct: 246 FHSFKKGDPDNPDLNPGLIIDIVGDIRAMNSEAVHAGSRKTGMIILGGGLPKHHVCNANM 305
Query: 274 FRGGTDYAIYISTAVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPILV 329
R G D+A+YI+TA +DGS SGA P E VSWGKI+ A V++ DAT+ FPILV
Sbjct: 306 MRNGADFAVYINTAQEFDGSDSGARPDEAVSWGKIRGGAKTVKVHCDATIAFPILV 361
>emb|CAB65461.1| (AJ238622) deoxyhypusine synthase [Senecio vernalis]
Length = 371
Score = 281 bits (711), Expect = 9e-75
Identities = 152/350 (43%), Positives = 219/350 (62%), Gaps = 33/350 (9%)
Query: 4 KDIVLKKSEEIEGLA--IEGPWLDEVESLEGVISYYEKIGFQATHLGKAVKIWRKVEEKR 61
+ +V K+SE +EG I G + ++ GFQA++LG A++ ++ + R
Sbjct: 15 RSVVFKESESLEGTCAKIRGYDFNNGIDYSQILKSMVSTGFQASNLGDAIETVNQMLDWR 74
Query: 62 KGGEEV-------------------RVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTA 102
E+V ++FLG+TSN++SSG+R+II +LV+ VDVIVTT
Sbjct: 75 LSHEQVTEDCSQEEKNPTYRESIKCKIFLGFTSNLISSGVRDIIRYLVQHHMVDVIVTTT 134
Query: 103 GGIEEDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILD 162
GGIEED IK L G++ + AELR KG+NRIGN+ VPND Y +FE +++P F+++L+
Sbjct: 135 GGIEEDLIKCLADTFKGEFSLPGAELRSKGLNRIGNLLVPNDNYCKFEDWIIPIFDQMLE 194
Query: 163 IERKLKRPL-TASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDML 221
E+K K L T S+ I +G+ ++ E S +YWAYK D+P+FCP +TDGS+GDML
Sbjct: 195 -EQKAKNVLWTPSKLIMRLGKEIN------NESSYLYWAYKNDIPVFCPGLTDGSLGDML 247
Query: 222 YFFKEERHDSKLVIDIANDIVKLNNLAITA--KETASIILGGSLPKHAIINANLFRGGTD 279
YF + L++D+ DI +N+ A+ A ++T IILGG LPKH I NAN+ R G D
Sbjct: 248 YF--HTFRNPGLIVDVVQDIRAINSEAVHANPRKTGMIILGGGLPKHHICNANMMRNGAD 305
Query: 280 YAIYISTAVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPILV 329
YA++I+TA +DGS SGA P E VSWGKI+ A V++ DAT+ FP+LV
Sbjct: 306 YAVFINTAQEFDGSDSGARPDEAVSWGKIRGSAKSVKVHCDATIAFPLLV 355
>sp|Q9VSF4|DHYS_DROME PROBABLE DEOXYHYPUSINE SYNTHASE (DHS) >gi|7295142|gb|AAF50467.1|
(AE003556) CG8005 gene product [Drosophila melanogaster]
Length = 411
Score = 277 bits (701), Expect = 1e-73
Identities = 150/350 (42%), Positives = 217/350 (61%), Gaps = 34/350 (9%)
Query: 3 AKDIVLKKSEEIEGLAIEGPWLDEVESLEGV-----ISYYEKIGFQATHLGKAVKIWRKV 57
AKD VLK+SE LA P + + EG+ Y GFQAT+LG A++ ++
Sbjct: 8 AKDAVLKRSE---ALAENTPQVSGYDFNEGLDYSKLFESYVNTGFQATNLGLAIREINRM 64
Query: 58 EEKR------------------KGGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIV 99
+ R + + VFLGYTSN+VSSGLRE I +L + R +D IV
Sbjct: 65 LDCRDQPLEADQIDSHETDDFIRRRSKCTVFLGYTSNLVSSGLRETIRFLAEHRMIDCIV 124
Query: 100 TTAGGIEEDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFER 159
TTAGG+EEDFIK L P +G +E++ +LRE+GINRIGN+ VPND Y +FE +++P +
Sbjct: 125 TTAGGVEEDFIKCLAPTFMGSFELSGRDLRERGINRIGNLLVPNDNYCKFEDWVMPLLDE 184
Query: 160 ILDIERKLKRPLTASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGD 219
+L+ ++ + S+ I+ +G + + SI YWA K +P+FCPA+TDGS+GD
Sbjct: 185 MLEEQKSQGTIWSPSKIIHRLGERIGD------PSSIYYWAAKNQIPVFCPALTDGSLGD 238
Query: 220 MLYFFKEERHDSKLVIDIANDIVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTD 279
M+YF LV+DI +D+ +LN +A+ A + II+GG + KH I NANL R G D
Sbjct: 239 MMYF--HSFRQPGLVVDILSDLRRLNTMAVKAVNSGMIIVGGGVIKHHICNANLMRNGAD 296
Query: 280 YAIYISTAVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPILV 329
Y+++I+TA +DGS SGA P E +SWGKI+ A V+++A+A+LVFP++V
Sbjct: 297 YSVFINTASEFDGSDSGARPDEAISWGKIRKDATPVKVYAEASLVFPLIV 346
>dbj|BAB10797.1| (AB017060) deoxyhypusine synthase [Arabidopsis thaliana]
Length = 368
Score = 277 bits (701), Expect = 1e-73
Identities = 152/347 (43%), Positives = 213/347 (60%), Gaps = 32/347 (9%)
Query: 7 VLKKSEEIEGLA--IEGPWLDEVESLEGVISYYEKIGFQATHLGKAVKI------WRKV- 57
V K+SE +EG IEG ++ ++ GFQA++LG+A+ + WR
Sbjct: 14 VFKESESLEGKCDKIEGYDFNQGVDYPKLMRSMLTTGFQASNLGEAIDVVNQMLDWRLAD 73
Query: 58 ----------EEKRKGGEE---VRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGG 104
EEK E ++FLG+TSN+VSSG+R+ I +LV+ VDVIVTT GG
Sbjct: 74 ETTVAEDCSEEEKNPSFRESVKCKIFLGFTSNLVSSGVRDTIRYLVQHHMVDVIVTTTGG 133
Query: 105 IEEDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIE 164
+EED IK L P GD+ + A LR KG+NRIGN+ VPND Y +FE +++P F+ +L +
Sbjct: 134 VEEDLIKCLAPTFKGDFSLPGAYLRSKGLNRIGNLLVPNDNYCKFEDWIIPIFDEMLKEQ 193
Query: 165 RKLKRPLTASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFF 224
++ T S+ + +G+ ++ E S +YWAYK ++P+FCP +TDGS+GDMLYF
Sbjct: 194 KEENVLWTPSKLLARLGKEIN------NESSYLYWAYKMNIPVFCPGLTDGSLGDMLYF- 246
Query: 225 KEERHDSKLVIDIANDIVKLNNLAITA--KETASIILGGSLPKHAIINANLFRGGTDYAI 282
S L+ID+ DI +N A+ A K+T IILGG LPKH I NAN+ R G DYA+
Sbjct: 247 -HSFRTSGLIIDVVQDIRAMNGEAVHANPKKTGMIILGGGLPKHHICNANMMRNGADYAV 305
Query: 283 YISTAVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPILV 329
+I+T +DGS SGA P E VSWGKI+ A V+++ DAT+ FP+LV
Sbjct: 306 FINTGQEFDGSDSGARPDEAVSWGKIRGSAKTVKVYCDATIAFPLLV 352
>pir||T39340 deoxyhypusine synthase (EC 1.1.1.249) SPBC1271.04c [similarity] -
fission yeast (Schizosaccharomyces pombe)
Length = 356
Score = 276 bits (699), Expect = 2e-73
Identities = 147/351 (41%), Positives = 217/351 (60%), Gaps = 30/351 (8%)
Query: 3 AKDIVLKKSEEIEGLAIE--GPWLDEVE----------SLEGVISYYEKIGFQATHLGKA 50
AKD V S + L E GP ++ + ++E ++ Y KIGFQAT+L A
Sbjct: 6 AKDAVFVASSKPSDLTTEVVGPDFNKYDERKKAGGPGITVEELVESYGKIGFQATNLHDA 65
Query: 51 VKI------WRKVEEKRKGGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGG 104
V I WR + + +FLGYTSN++SSG+RE++ +LV+ + VDVIVTTAGG
Sbjct: 66 VTIINEMRNWRDPNPPEEKSDRATIFLGYTSNLISSGVREVLRYLVQHKCVDVIVTTAGG 125
Query: 105 IEEDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIE 164
+EED IK L P +GD+ ++ LR KG+NRIGN+ VPND Y FE+++ P ++++ +
Sbjct: 126 VEEDIIKCLGPTYVGDFHLDGKNLRAKGLNRIGNLIVPNDNYCRFEEWIFPILNKMVEEQ 185
Query: 165 RKLKRPLTASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFF 224
L T S FI +G+ +++ E S++YWAYK ++PI+ PA+TDGSIGDMLYF
Sbjct: 186 ETLGTHWTPSSFIRRLGKEIND------ESSVLYWAYKNNIPIYSPALTDGSIGDMLYFH 239
Query: 225 KEERHDSKLVIDIANDIVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYI 284
+ LV+DI DI +N A+ A ++ IILGG + KH I NANL R G ++++YI
Sbjct: 240 TYKATPRPLVLDIVADIRNMNTHAVRANKSGIIILGGGVVKHHICNANLMRNGAEWSVYI 299
Query: 285 STAVPWDGSLSGAPPSEGVSWGKIKAKA------DYVEIWADATLVFPILV 329
++A +DGS +GA P E VSWGKI+ A D + ++ + +L+FP++V
Sbjct: 300 NSANEFDGSDAGARPDEAVSWGKIRGDAKKVKVCDAILVYGEVSLLFPLIV 350
>gb|AAG19745.1| (AE005060) deoxyhypusine synthase; Dhs [Halobacterium sp. NRC-1]
Length = 330
Score = 276 bits (698), Expect = 3e-73
Identities = 146/300 (48%), Positives = 203/300 (67%), Gaps = 19/300 (6%)
Query: 33 VISYYEKIGFQATHLGKAVKIWRKVEEKRKGGEEVRVFLGYTSNIVSSGLREIIAWLVKE 92
++ Y GFQA+HL AV I R++ E ++ ++L TSNIVSSGLRE++A LV+E
Sbjct: 40 LLDSYATTGFQASHLADAVDITREMRE-----DDATIYLTLTSNIVSSGLREVVAHLVRE 94
Query: 93 RKVDVIVTTAGGIEEDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKY 152
VDVI+TT+G + ED IKT KPF +G+W+V++A LRE+GINR+GNIFVP+DRY+ E+Y
Sbjct: 95 NYVDVIITTSGSLTEDIIKTAKPFKMGEWDVDEAALREEGINRLGNIFVPSDRYVWLEEY 154
Query: 153 MVPFFERILDIERKLKRPLTASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAI 212
+ FFE ++K++ P + F E+G +D+ E SI+ A D+P+FCPA+
Sbjct: 155 LYDFFEEFF-ADQKVRTP---TAFARELGATLDD------EDSILKNAADNDIPVFCPAL 204
Query: 213 TDGSIGDMLYFFKEERHDSKLVIDIANDIVKLNNLAITAKETASIILGGSLPKHAIINAN 272
TD IG+ LY+++ + +DS++ I+I +D L + A T I +G +PKH I N
Sbjct: 205 TDAEIGNFLYYYR-QGYDSEVGIEILDDYDALIEEGLLADTTGLICVGAGVPKHHAIMTN 263
Query: 273 LFRGGTDYAIYISTAVPWDGSLSGAPPSEGVSWGKIK---AKADYVEIWADATLVFPILV 329
LFRGG DYA+YIST + DGSLSGAPP E VSWGKIK A+ +Y I A+ATLVFP+LV
Sbjct: 264 LFRGGADYAVYISTGMEGDGSLSGAPPEEAVSWGKIKDEDAEPNYALIEAEATLVFPLLV 323
>pdb|1DHS| Crystal Structure Of The Nad Complex Of Human Deoxyhypusine
Synthase
Length = 344
Score = 272 bits (688), Expect = 4e-72
Identities = 145/326 (44%), Positives = 209/326 (63%), Gaps = 11/326 (3%)
Query: 7 VLKKSEEI--EGLAIEGPWLDEVESLEGVISYYEKIGFQATHLGKAVK-IWRKVEEKRKG 63
VLK S + E + G + + ++ + GFQAT+ G+AV+ + +E+K +
Sbjct: 9 VLKHSSTLPPESTQVRGYDFNRGVNYRALLEAFGTTGFQATNFGRAVQQVNAMIEKKLEP 68
Query: 64 GEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGGIEEDFIKTLKPFILGDWEV 123
+FLGYTSN++SSG+RE I +LV+ VDV+VTTAGG+EED IK L P LG++ +
Sbjct: 69 LTSCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTTAGGVEEDLIKCLAPTYLGEFSL 128
Query: 124 NDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIERKLKRPLTASEFIYEMGRY 183
ELRE GINRIGN+ VPN+ Y +FE +++P ++++ + T S+ I +G+
Sbjct: 129 RGKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMVMEQNTEGVKWTPSKMIARLGKE 188
Query: 184 MDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFFKEERHDSKLVIDIANDIVK 243
++ +S+ YWA K +P+F PA+TDGS+GDM++F + + LV+DI D+
Sbjct: 189 IN------NPESVYYWAQKNHIPVFSPALTDGSLGDMIFFHSYK--NPGLVLDIVEDLRL 240
Query: 244 LNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYISTAVPWDGSLSGAPPSEGV 303
+N AI AK T IILGG + KH I NANL R G DYA+YI+TA +DGS SGA P E V
Sbjct: 241 INTQAIFAKCTGMIILGGGVVKHHIANANLMRNGADYAVYINTAQEFDGSDSGARPDEAV 300
Query: 304 SWGKIKAKADYVEIWADATLVFPILV 329
SWGKI+ A V+++ADA+LVFP+LV
Sbjct: 301 SWGKIRVDAQPVKVYADASLVFPLLV 326
>sp|Q9XXJ0|DHYS_CAEEL PROBABLE DEOXYHYPUSINE SYNTHASE (DHS) >gi|7509439|pir||T26497
deoxyhypusine synthase (EC 1.1.1.249) Y17G7B.4
[similarity] - Caenorhabditis elegans
>gi|3947599|emb|CAA19451.1| (AL023828) contains
similarity to Pfam domain: PF01916 (Deoxyhypusine
synthase), Score=571.1, E-value=2.3e-168, N=1~cDNA EST
yk115d9.3 comes from this gene~cDNA EST yk60f7.3 comes
from this gene~cDNA EST yk60f7.5 comes from this
gene~cDNA EST yk115d9.5>
Length = 371
Score = 266 bits (674), Expect = 2e-70
Identities = 141/321 (43%), Positives = 197/321 (60%), Gaps = 29/321 (9%)
Query: 29 SLEGVISYYEKIGFQATHLGKAV--------------------KIWRKVEEKRKGGEEVR 68
SL ++S Y GFQATHL +A+ K++ E ++K
Sbjct: 48 SLSAILSSYMSTGFQATHLAQAIQQVNQMLSLRDTPLTCDDDEKLFPYPEGRQK--RSCT 105
Query: 69 VFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGGIEEDFIKTLKPFILGDWEVNDAEL 128
+FLGYTSN+V+SGLRE++ + V+ VD IVT+AGGIEED IK LKP LG + ++ A+L
Sbjct: 106 IFLGYTSNLVTSGLREVLRYCVQRNLVDCIVTSAGGIEEDLIKCLKPSYLGTFTMDGAKL 165
Query: 129 REKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIERKLKRPLTASEFIYEMGRYMDEVL 188
R G+NR GN+ +PND Y FE +++P + L + + T S+ I +G + +
Sbjct: 166 RSNGMNRAGNVLIPNDNYCAFEDWLMPILDECLVEQEEKHLNWTPSKLIQRLGERIGD-- 223
Query: 189 GKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFFKEERHDSKLVIDIANDIVKLNNLA 248
E SI+YWA K +P+FCPA+TDGS+GDMLY F + L +DI D+ +N +A
Sbjct: 224 ----ESSILYWAAKHRIPVFCPALTDGSLGDMLY-FHSVKSSPGLRVDIVEDVRHINTIA 278
Query: 249 ITAKETASIILGGSLPKHAIINANLFRGGTDYAIYISTAVPWDGSLSGAPPSEGVSWGKI 308
+ + +T SIILGG + KH I NANL R G D+ +YI+T +DGS SGA P E VSWGK+
Sbjct: 279 VKSFKTGSIILGGGVVKHHINNANLMRNGADHTVYINTGQEFDGSDSGAQPDEAVSWGKV 338
Query: 309 KAKADYVEIWADATLVFPILV 329
K A V++ A+ATLVFP+LV
Sbjct: 339 KPSAGAVKVHAEATLVFPLLV 359
>pir||T51022 deoxyhypusine synthase (EC 1.1.1.249) B7F21.30 [similarity] -
Neurospora crassa >gi|9367486|emb|CAB97475.1| (AL389901)
DEOXYHYPUSINE SYNTHASE [Neurospora crassa]
Length = 353
Score = 266 bits (674), Expect = 2e-70
Identities = 143/329 (43%), Positives = 210/329 (63%), Gaps = 19/329 (5%)
Query: 9 KKSEEIEGLAIEGPWLDEVESLEGVISYYEKIGFQATHLGKAVKIWRKVEEKRKG--GEE 66
+K EE++ +G + + L+G+ + +GFQA+ + +AV+I ++ R E+
Sbjct: 26 QKVEELDFNKFKGRPITVDDLLQGM----KHMGFQASSMCEAVRIINEMRAYRDPTTSEK 81
Query: 67 VRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGGIEEDFIKTLKPFILGDWEVNDA 126
+FLGYTSN++SSGLR + +LV+ + V IVTTAGGIEEDFIK L + + A
Sbjct: 82 TTIFLGYTSNLISSGLRGTLRYLVQHKHVSAIVTTAGGIEEDFIKCLGDTYMSSFSAVGA 141
Query: 127 ELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIERKLKRPL------TASEFIYEM 180
+LR KG+NRIGN+ VPN Y FE ++VP +++L+ E++ R T S+ I+ +
Sbjct: 142 DLRSKGLNRIGNLVVPNSNYCAFEDWVVPILDKMLE-EQEASRGTENEINWTPSKVIHRL 200
Query: 181 GRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFFKEERHDSKLVIDIAND 240
G+ +++ E+S+ YWA+K D+P+FCPA+TDGS+GDMLYF + +L IDI D
Sbjct: 201 GKEIND------ERSVYYWAWKNDIPVFCPALTDGSLGDMLYFHTFKASPKQLRIDIVED 254
Query: 241 IVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYISTAVPWDGSLSGAPPS 300
I K+N +A+ AK IILGG + KH I NA L R G + A+YI+TA +DGS +GA P
Sbjct: 255 IRKINTIAVRAKRAGMIILGGGIVKHHIANACLMRNGAESAVYINTAQEFDGSDAGARPD 314
Query: 301 EGVSWGKIKAKADYVEIWADATLVFPILV 329
E VSWGKIK AD V+++ +AT FP +V
Sbjct: 315 EAVSWGKIKVGADAVKVYMEATAAFPFIV 343
>gi|4503325 deoxyhypusine synthase isoform a [Homo sapiens]
>gi|11525967|ref|XP_009070.1| deoxyhypusine synthase
isoform a [Homo sapiens]
>gi|1352267|sp|P49366|DHYS_HUMAN DEOXYHYPUSINE SYNTHASE
(DHS) >gi|994715|gb|AAA86282.1| (L39068) deoxyhypusine
synthase [Homo sapiens] >gi|1710220|gb|AAB50208.1|
(U79262) deoxyhypusine synthase [Homo sapiens]
>gi|3021398|emb|CAA04940.1| (AJ001701) deoxyhypusine
synthase [Homo sapiens]
Length = 369
Score = 266 bits (673), Expect = 3e-70
Identities = 147/343 (42%), Positives = 211/343 (60%), Gaps = 28/343 (8%)
Query: 7 VLKKSEEI--EGLAIEGPWLDEVESLEGVISYYEKIGFQATHLGKAVK-----IWRKVE- 58
VLK S + E + G + + ++ + GFQAT+ G+AV+ I +K+E
Sbjct: 17 VLKHSSTLPPESTQVRGYDFNRGVNYRALLEAFGTTGFQATNFGRAVQQVNAMIEKKLEP 76
Query: 59 ------------EKRKGGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGGIE 106
+ R+ +FLGYTSN++SSG+RE I +LV+ VDV+VTTAGG+E
Sbjct: 77 LSQDEDQHADLTQSRRPLTSCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTTAGGVE 136
Query: 107 EDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIERK 166
ED IK L P LG++ + ELRE GINRIGN+ VPN+ Y +FE +++P ++++ +
Sbjct: 137 EDLIKCLAPTYLGEFSLRGKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMVMEQNT 196
Query: 167 LKRPLTASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFFKE 226
T S+ I +G+ ++ +S+ YWA K +P+F PA+TDGS+GDM++F
Sbjct: 197 EGVKWTPSKMIARLGKEIN------NPESVYYWAQKNHIPVFSPALTDGSLGDMIFFHSY 250
Query: 227 ERHDSKLVIDIANDIVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYIST 286
+ + LV+DI D+ +N AI AK T IILGG + KH I NANL R G DYA+YI+T
Sbjct: 251 K--NPGLVLDIVEDLRLINTQAIFAKCTGMIILGGGVVKHHIANANLMRNGADYAVYINT 308
Query: 287 AVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPILV 329
A +DGS SGA P E VSWGKI+ A V+++ADA+LVFP+LV
Sbjct: 309 AQEFDGSDSGARPDEAVSWGKIRVDAQPVKVYADASLVFPLLV 351
>pir||S68692 deoxyhypusine synthase (EC 1.1.1.249) [validated] - human
>gi|1113109|gb|AAA96151.1| (U40579) deoxyhypusine
synthase [Homo sapiens]
Length = 369
Score = 266 bits (673), Expect = 3e-70
Identities = 147/343 (42%), Positives = 211/343 (60%), Gaps = 28/343 (8%)
Query: 7 VLKKSEEI--EGLAIEGPWLDEVESLEGVISYYEKIGFQATHLGKAVK-----IWRKVE- 58
VLK S + E + G + + ++ + GFQAT+ G+AV+ I +K+E
Sbjct: 17 VLKHSSTLPPESTQVRGYDFNRGVNYRALLEAFGTTGFQATNFGRAVQQVNAMIEKKLEP 76
Query: 59 ------------EKRKGGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGGIE 106
+ R+ +FLGYTSN++SSG+RE I +LV+ VDV+VTTAGG+E
Sbjct: 77 LSQDEDQHADLTQSRRPLTSCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTTAGGVE 136
Query: 107 EDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIERK 166
ED IK L P LG++ + ELRE GINRIGN+ VPN+ Y +FE +++P ++++ +
Sbjct: 137 EDLIKCLAPTYLGEFSLRGKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMVMEQNT 196
Query: 167 LKRPLTASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFFKE 226
T S+ I +G+ ++ +S+ YWA K +P+F PA+TDGS+GDM++F
Sbjct: 197 EGVKWTPSKMIARLGKEIN------NPESVYYWAQKNHIPVFSPALTDGSLGDMIFFHSY 250
Query: 227 ERHDSKLVIDIANDIVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYIST 286
+ + LV+DI D+ +N AI AK T IILGG + KH I NANL R G DYA+YI+T
Sbjct: 251 K--NPGLVLDIVEDLRLINTQAIFAKCTGMIILGGGVVKHHIANANLMRNGADYAVYINT 308
Query: 287 AVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPILV 329
A +DGS SGA P E VSWGKI+ A V+++ADA+LVFP+LV
Sbjct: 309 AQEFDGSDSGARPDEAVSWGKIRVDAQPVKVYADASLVFPLLV 351
>sp|P49365|DHYS_NEUCR DEOXYHYPUSINE SYNTHASE (DHS) >gi|11280362|pir||T47195 deoxyhypusine
synthase (EC 1.1.1.249) [similarity] - Neurospora crassa
>gi|1019423|gb|AAC49075.1| (U22400) deoxyhypusine
synthase [Neurospora crassa]
Length = 353
Score = 265 bits (671), Expect = 4e-70
Identities = 142/329 (43%), Positives = 210/329 (63%), Gaps = 19/329 (5%)
Query: 9 KKSEEIEGLAIEGPWLDEVESLEGVISYYEKIGFQATHLGKAVKIWRKVEEKRKG--GEE 66
+K EE++ +G + + L+G+ + +GFQA+ + +AV+I ++ R E+
Sbjct: 26 QKVEELDFNKFKGRPITVDDLLQGM----KHMGFQASSMCEAVRIINEMRAYRDPTTSEK 81
Query: 67 VRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGGIEEDFIKTLKPFILGDWEVNDA 126
+FLGYTSN++SSGLR + +LV+ + V IVTTAGGIEEDFIK L + + A
Sbjct: 82 TTIFLGYTSNLISSGLRGTLRYLVQHKHVSAIVTTAGGIEEDFIKCLGDTYMSSFSAVGA 141
Query: 127 ELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIERKLKRPL------TASEFIYEM 180
+LR KG+NRIGN+ VPN Y FE ++VP +++L+ E++ R T S+ I+ +
Sbjct: 142 DLRSKGLNRIGNLVVPNSNYCAFEDWVVPILDKMLE-EQEASRGTENEINWTPSKVIHRL 200
Query: 181 GRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFFKEERHDSKLVIDIAND 240
G+ +++ E+S+ YWA+K D+P+FCPA+TDGS+GDMLYF + +L IDI D
Sbjct: 201 GKEIND------ERSVYYWAWKNDIPVFCPALTDGSLGDMLYFHTFKASPEQLRIDIVED 254
Query: 241 IVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYISTAVPWDGSLSGAPPS 300
I K+N +A+ AK IILGG + KH I NA L R G + A+YI+TA +DGS +G+ P
Sbjct: 255 IRKINTIAVRAKRAGMIILGGGIVKHHIANACLMRNGAESAVYINTAQEFDGSDAGSRPD 314
Query: 301 EGVSWGKIKAKADYVEIWADATLVFPILV 329
E VSWGKIK AD V+++ +AT FP +V
Sbjct: 315 EAVSWGKIKVGADAVKVYMEATAAFPFIV 343
>gi|6321859 Deoxyhypusine synthase; Dys1p [Saccharomyces cerevisiae]
>gi|731670|sp|P38791|DHYS_YEAST DEOXYHYPUSINE SYNTHASE
(DHS) >gi|626626|pir||S46698 deoxyhypusine synthase (EC
1.1.1.249) [validated] - yeast (Saccharomyces
cerevisiae) >gi|487946|gb|AAB68377.1| (U00061) Dys1p:
Deoxyhypusine synthase [Saccharomyces cerevisiae]
>gi|1375088|dbj|BAA11253.1| (D78185) deoxyhypusine
synthase [Saccharomyces cerevisiae]
Length = 387
Score = 262 bits (663), Expect = 4e-69
Identities = 144/362 (39%), Positives = 222/362 (60%), Gaps = 38/362 (10%)
Query: 4 KDIVLKKSE-------EIEGLAIEGPWLDEVESLEGVISYYEKIGFQATHLGKAVKI--- 53
+D VLK S +++G+ P + + + +I + +GFQA+ +G A +I
Sbjct: 13 QDAVLKASVPIPDDFVKVQGIDYSKPEATNMRATD-LIEAMKTMGFQASSVGTACEIIDS 71
Query: 54 ---WR-----KVEEKRKGG-------EEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVI 98
WR ++++ K G ++ +F+GYTSN++SSG+RE + +LV+ + VD +
Sbjct: 72 MRSWRGKHIDELDDHEKKGCFDEEGYQKTTIFMGYTSNLISSGVRETLRYLVQHKMVDAV 131
Query: 99 VTTAGGIEEDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFE 158
VT+AGG+EED IK L P LG++ + LR++G+NRIGN+ VPND Y +FE+++VP +
Sbjct: 132 VTSAGGVEEDLIKCLAPTYLGEFALKGKSLRDQGMNRIGNLLVPNDNYCKFEEWIVPILD 191
Query: 159 RILDIERK---------LKRPLTASEFIYEMGRYMDEVLGKE--KEKSIIYWAYKRDVPI 207
++L+ + + L+ I+ + +D GKE E S++YWA+K +PI
Sbjct: 192 KMLEEQDEYVKKHGADCLEANQDVDSPIWTPSKMIDR-FGKEINDESSVLYWAHKNKIPI 250
Query: 208 FCPAITDGSIGDMLYFFKEERHDSKLVIDIANDIVKLNNLAITAKETASIILGGSLPKHA 267
FCP++TDGSIGDML+F + +L +DI DI K+N++++ A IILGG L KH
Sbjct: 251 FCPSLTDGSIGDMLFFHTFKASPKQLRVDIVGDIRKINSMSMAAYRAGMIILGGGLIKHH 310
Query: 268 IINANLFRGGTDYAIYISTAVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPI 327
I NA L R G DYA+YI+T +DGS +GA P E VSWGKIKA+A V+++AD T V P+
Sbjct: 311 IANACLMRNGADYAVYINTGQEYDGSDAGARPDEAVSWGKIKAEAKSVKLFADVTTVLPL 370
Query: 328 LV 329
+V
Sbjct: 371 IV 372
>pir||S71138 deoxyhypusine synthase long form - human >gi|1335786|gb|AAB02179.1|
(U32178) deoxyhypusine synthase [Homo sapiens]
Length = 369
Score = 257 bits (649), Expect = 2e-67
Identities = 146/349 (41%), Positives = 211/349 (59%), Gaps = 29/349 (8%)
Query: 2 KAKDIVLKKSEEI--EGLAIEGPWLDEVESLEGVISYYEKIGFQATHLGKAVK-----IW 54
+ + VLK S + E + G + + ++ + GFQAT+ G+AV+ I
Sbjct: 11 RGRAAVLKHSSTLPPESTQVRGYDFNRGVNYRALLEAFGTTGFQATNFGRAVQQVNAMIE 70
Query: 55 RKVE-------------EKRKGGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTT 101
+K+E + R+ +FLGYTSN++SSG+RE I +LV+ VDV+VTT
Sbjct: 71 KKLEPLSQDEDQHGDLTQSRRPLTSCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTT 130
Query: 102 AGGIEEDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERIL 161
AGG+EED IK L P LG++ + ELRE GINRIGN+ VPN+ Y +FE +++P ++++
Sbjct: 131 AGGVEEDLIKCLAPTYLGEFSLRGKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMV 190
Query: 162 DIERKLKRPLTASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDML 221
+ T S+ I +G+ ++ +S YWA K +P+F PA+TDGS+GDM+
Sbjct: 191 MEQNIEGAKWTPSKMIARLGKEIN------NPESAYYWAQKNHIPVFSPALTDGSLGDMI 244
Query: 222 YFFKEERHDSKLVIDIANDIVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYA 281
+F + + LV+DI D+ +N AI AK T IILGG + KH I NANL G DYA
Sbjct: 245 FFHSYK--NPGLVLDIVEDLRLINTQAIFAKCTGMIILGGGVVKHHIANANLSGNGADYA 302
Query: 282 IYISTA-VPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPILV 329
+YI+TA +DGS SGA P E VSWGKI+ A V+++ADA+LVFP+LV
Sbjct: 303 VYINTAQEEFDGSDSGARPDEAVSWGKIRVDAQPVKVYADASLVFPLLV 351
>emb|CAB95376.1| (AL359782) possible deoxyhypusine synthase [Trypanosoma brucei]
Length = 342
Score = 210 bits (528), Expect = 3e-53
Identities = 113/311 (36%), Positives = 176/311 (56%), Gaps = 12/311 (3%)
Query: 24 LDEVESLEGVISYYEKIGFQATHLGKAVKIWRKVEEKRKGGEEVRVFLGYTSNIVSSGLR 83
+ + E++ V++ Y IG +AT+LG+A +I ++ G + +V L YTSN++SSGLR
Sbjct: 22 IGQEEAIRCVLNAYPNIGLEATNLGRARRIVQRALNDN-GMDGNKVMLAYTSNLISSGLR 80
Query: 84 EIIAWLVKERKVDVIVTTAGGIEEDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPN 143
+ A L +E ++ +VTTAGG+EED IK L ++GD+ +ND LR G+NR+GN+ VPN
Sbjct: 81 DTFACLARENRIGAVVTTAGGVEEDVIKCLGDTLVGDFALNDHALRNNGLNRVGNLLVPN 140
Query: 144 DRYIEFEKYMVPFFERILDIER--KLKRPLTASEFIYEMGRYMDEVLGKEKEKSIIYWAY 201
D Y FE + VP R+ + +R + T S+ I E+G ++ V + S+IYW Y
Sbjct: 141 DNYRNFEDFFVPLLRRLHEQQRDSRWTTKTTPSQIIAEIGAALESVRPNDCGSSLIYWCY 200
Query: 202 KRDVPIFCPAITDGSIGDMLYFFKEERHDSKLVIDIANDIVKLNNLAITAKET---ASII 258
+ D+P+F PA TDGS+GDM+YF+ R LV+D D+ +L L + I+
Sbjct: 201 RNDIPVFSPAFTDGSMGDMIYFYNYSR--KGLVVDPVPDVRRLRQLGCKSTNVGRITCIV 258
Query: 259 LGGSLPKHAIINANLFRGGTDYAIYISTAVPWDGSLSGAPPSEGVSWGKIKAKADYVEIW 318
LG LPKH ++ D +Y++T DG S + G + D V +
Sbjct: 259 LGAGLPKHHLLR----NVQADAVVYVTTGSDADGCESSCNVMADRANGLLSPNCDVVRVH 314
Query: 319 ADATLVFPILV 329
DAT++ P+L+
Sbjct: 315 GDATIISPLLL 325
>emb|CAC11500.1| (AL445064) deoxyhypusine synthase related protein [Thermoplasma
acidophilum]
Length = 333
Score = 203 bits (512), Expect = 2e-51
Identities = 111/292 (38%), Positives = 177/292 (60%), Gaps = 22/292 (7%)
Query: 41 GFQATHLGKAVKIWRKVEEKRKGGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVT 100
GF A + +A +I + + E+ FL + ++I+S+GLR +I +VK + VDVI+T
Sbjct: 57 GFTAAKIHEAYEIIKDMFS-----EDNTTFLSFPADIISTGLRGLINEVVKRKLVDVIIT 111
Query: 101 TAGGIEEDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERI 160
T+G ++ D +T + + G + +D ELR+ GINR+GN+ VP++ Y E + E++
Sbjct: 112 TSGTLDHDIARTYRNYYCGSFSYSDIELRDLGINRLGNVLVPDESYGEIIE------EKV 165
Query: 161 LDIERKL---KRPLTASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSI 217
++ KL K+ + I+E+G L E SIIY A K ++P+F P ITDGS
Sbjct: 166 MESLEKLYAKKKEWATVDLIHEVG------LDINSESSIIYNAAKNNIPVFVPGITDGSF 219
Query: 218 GDMLYFFKEERHDSKLVIDIANDIVKLNNLAITAKETASIILGGSLPKHAIINANLFRGG 277
G L+ F E+ HD K I++ D +L+++ AK+T +I++GG + KH I N FR G
Sbjct: 220 GSQLWSFYEQHHDFK--INLLEDEHRLSDIIFDAKKTGAIMIGGGISKHHTIWWNQFRDG 277
Query: 278 TDYAIYISTAVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPILV 329
DYA+Y++TA +DGSLSGA E +SW K++ A YV ++ DAT++ P+L+
Sbjct: 278 LDYAVYVTTAQEYDGSLSGAKLEEAISWKKVRPDARYVNVYGDATVIMPVLL 329
>gi|7108338 deoxyhypusine synthase isoform a [Homo sapiens]
>gi|11525969|ref|XP_009071.1| deoxyhypusine synthase
isoform b [Homo sapiens]
Length = 322
Score = 193 bits (486), Expect = 2e-48
Identities = 120/343 (34%), Positives = 179/343 (51%), Gaps = 75/343 (21%)
Query: 7 VLKKSEEI--EGLAIEGPWLDEVESLEGVISYYEKIGFQATHLGKAVK-----IWRKVE- 58
VLK S + E + G + + ++ + GFQAT+ G+AV+ I +K+E
Sbjct: 17 VLKHSSTLPPESTQVRGYDFNRGVNYRALLEAFGTTGFQATNFGRAVQQVNAMIEKKLEP 76
Query: 59 ------------EKRKGGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGGIE 106
+ R+ +FLGYTSN++SSG+RE I +LV+ VDV+VTTAGG+E
Sbjct: 77 LSQDEDQHADLTQSRRPLTSCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTTAGGVE 136
Query: 107 EDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIERK 166
ED IK L P LG++ + ELRE GINRIGN+ VPN+ Y +FE +++P ++++ +
Sbjct: 137 EDLIKCLAPTYLGEFSLRGKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMVMEQNT 196
Query: 167 LKRPLTASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFFKE 226
T S+ I +G+ ++ +S+ YWA K +P+F PA+TDGS+GDM++F
Sbjct: 197 EGVKWTPSKMIARLGKEIN------NPESVYYWAQKNHIPVFSPALTDGSLGDMIFFHSY 250
Query: 227 ERHDSKLVIDIANDIVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYIST 286
+ + LV+DI
Sbjct: 251 K--NPGLVLDIVE----------------------------------------------- 261
Query: 287 AVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPILV 329
A +DGS SGA P E VSWGKI+ A V+++ADA+LVFP+LV
Sbjct: 262 AQEFDGSDSGARPDEAVSWGKIRVDAQPVKVYADASLVFPLLV 304
>gb|AAB02175.1| (U26266) deoxyhypusine synthase [Homo sapiens]
Length = 321
Score = 192 bits (483), Expect = 5e-48
Identities = 120/348 (34%), Positives = 180/348 (51%), Gaps = 75/348 (21%)
Query: 2 KAKDIVLKKSEEI--EGLAIEGPWLDEVESLEGVISYYEKIGFQATHLGKAVK-----IW 54
+ + VLK S + E + G + + ++ + GFQAT+ G+AV+ I
Sbjct: 11 RGRAAVLKHSSTLPPESTQVRGYDFNRGVNYRALLEAFGTTGFQATNFGRAVQQVNAMIE 70
Query: 55 RKVE-------------EKRKGGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTT 101
+K+E + R+ +FLGYTSN++SSG+RE I +LV+ VDV+VTT
Sbjct: 71 KKLEPLSQDEDQHGDLTQSRRPLTSCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTT 130
Query: 102 AGGIEEDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERIL 161
AGG+EED IK L P LG++ + ELRE GINRIGN+ VPN+ Y +FE +++P ++++
Sbjct: 131 AGGVEEDLIKCLAPTYLGEFSLRGKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMV 190
Query: 162 DIERKLKRPLTASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDML 221
+ T S+ I +G+ ++ +S YWA K +P+F PA+TDGS+GDM+
Sbjct: 191 MEQNIEGAKWTPSKMIARLGKEIN------NPESAYYWAQKNHIPVFSPALTDGSLGDMI 244
Query: 222 YFFKEERHDSKLVIDIANDIVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYA 281
+F + + LV+DI
Sbjct: 245 FFHSYK--NPGLVLDIVE------------------------------------------ 260
Query: 282 IYISTAVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPILV 329
A +DGS SGA P E VSWGKI+ A V+++ADA+LVFP+LV
Sbjct: 261 -----AQEFDGSDSGARPDEAVSWGKIRVDAQPVKVYADASLVFPLLV 303
>gi|7108340 deoxyhypusine synthase isoform a [Homo sapiens]
>gi|11525971|ref|XP_009069.1| deoxyhypusine synthase
isoform c [Homo sapiens]
Length = 313
Score = 176 bits (443), Expect = 2e-43
Identities = 114/343 (33%), Positives = 173/343 (50%), Gaps = 84/343 (24%)
Query: 7 VLKKSEEI--EGLAIEGPWLDEVESLEGVISYYEKIGFQATHLGKAVK-----IWRKVE- 58
VLK S + E + G + + ++ + GFQAT+ G+AV+ I +K+E
Sbjct: 17 VLKHSSTLPPESTQVRGYDFNRGVNYRALLEAFGTTGFQATNFGRAVQQVNAMIEKKLEP 76
Query: 59 ------------EKRKGGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGGIE 106
+ R+ +FLGYTSN++SSG+RE I +LV+ VDV+VTTAGG+E
Sbjct: 77 LSQDEDQHADLTQSRRPLTSCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTTAGGVE 136
Query: 107 EDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIERK 166
ED IK L P LG++ + ELRE GINRIGN+ VPN+ Y +FE +++P ++++ +
Sbjct: 137 EDLIKCLAPTYLGEFSLRGKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMVMEQNT 196
Query: 167 LKRPLTASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFFKE 226
T S+ I +G+ ++ +S+ YWA K +P+F PA+TDGS+GDM++F
Sbjct: 197 EGVKWTPSKMIARLGKEIN------NPESVYYWAQKNHIPVFSPALTDGSLGDMIFFHSY 250
Query: 227 ERHDSKLVIDIANDIVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYIST 286
+ + LV+DI G+ P A
Sbjct: 251 K--NPGLVLDIVE---------------------GARPDEA------------------- 268
Query: 287 AVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPILV 329
VSWGKI+ A V+++ADA+LVFP+LV
Sbjct: 269 ----------------VSWGKIRVDAQPVKVYADASLVFPLLV 295
>sp|Q9YE72|DHYS_AERPE PROBABLE DEOXYHYPUSINE SYNTHASE (DHS) >gi|7451181|pir||B72659
deoxyhypusine synthase (EC 1.1.1.249) APE0698
[validated] - Aeropyrum pernix (strain K1)
>gi|5104359|dbj|BAA79674.1| (AP000060) 292aa long
hypothetical deoxyhypusine synthase [Aeropyrum pernix]
Length = 292
Score = 175 bits (439), Expect = 7e-43
Identities = 102/300 (34%), Positives = 172/300 (57%), Gaps = 17/300 (5%)
Query: 36 YYEKIGFQATHLGKAVKIWRKVEEKRKGGEEVRVFLGYTSNIVSSGLREIIAWLVKERKV 95
Y + GF A HL +AV++ R+ E VRV L +T N+V++GLR ++A L+
Sbjct: 4 YRDIHGFMAGHLAEAVEVLREGLE----ASSVRV-LTFTGNLVATGLRGVLAQLIDGGLF 58
Query: 96 DVIVTTAGGIEEDFIKTLK-PFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMV 154
+V+ TTAG ++ D + + ++ G +E +D EL +G++R+GN+F+P + Y
Sbjct: 59 NVVFTTAGALDHDIARFMGGKYLKGRFEADDTELHRRGVHRLGNVFIPVESY-------G 111
Query: 155 PFFERILDIERKLKRPLTASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITD 214
P ER + + + +YE+ R ++ +++ SI+ A +R V +F P D
Sbjct: 112 PLVERFVRTLAEQAAGVRGEWGVYELLRLAGSLMEGDRD-SILAAAARRGVDVFVPGWPD 170
Query: 215 GSIGDMLYFFKEERHDSKLVIDIANDIVKLNNLAITAK-ETASIILGGSLPKHAIINANL 273
G+ G L F E + + + +D D+ +L ++ + E A++I+GG + KH I +
Sbjct: 171 GAFGTSL--FMERQRGTSITVDYFRDMARLADIFFPQEGEAAALIVGGGISKHHAIWWSQ 228
Query: 274 FRGGTDYAIYISTAVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPILVWMVM 333
FRGG DYA+Y++TAV +DGSLSGA P E VSWGKIK + V ++ DAT+ P++ + ++
Sbjct: 229 FRGGLDYAVYVTTAVEYDGSLSGAHPREAVSWGKIKESSRRVVVYGDATITLPVIAYCLL 288
>gb|AAK41241.1| Deoxyhypusine synthase [Sulfolobus solfataricus]
Length = 312
Score = 170 bits (426), Expect = 2e-41
Identities = 101/312 (32%), Positives = 180/312 (57%), Gaps = 19/312 (6%)
Query: 19 IEGPWLDEVESLEGVISYYEKI-GFQATHLGKAVKIWRKVEEKRKGGEEVRVFLGYTSNI 77
+E L +++ ++S ++KI GF + + KI +++ + ++R FL +T+N+
Sbjct: 12 VEDITLSDLKKYNDIVSVFDKIYGFSSEGIVNGSKILKEMIKNA----DLR-FLSFTANL 66
Query: 78 VSSGLREIIAWLVKERKVDVIVTTAGGIEEDFIKTLKP-FILGDWEVNDAELREKGINRI 136
VS+GLR + A L+K+ +++VTT G I+ D ++ + G ++++D L++ I+R+
Sbjct: 67 VSTGLRGLFADLIKKGYFNIVVTTGGTIDHDLARSFGGVYYKGSFDIDDTMLKDLEIHRL 126
Query: 137 GNIFVPNDRYIEFEKYMVPFFERILDIERKLKRPLTASEFIYEMGRYMDEVLGKEKEKSI 196
GN+ VP + Y K + + L K ++ ++A E ++E G+ + + SI
Sbjct: 127 GNVLVPFESY---GKVIEDVVRKFLPEITKDRKEISAYELLWEFGKRITD------SNSI 177
Query: 197 IYWAYKRDVPIFCPAITDGSIGDMLYFFKEERHDSKLVIDIANDIVKLNNLAITAKETAS 256
+ AY ++VPI P I DGS G L+ + I++ D+ + +L ++K++ +
Sbjct: 178 LRAAYDKNVPIIVPGILDGSFGTNLFI---QSQFLNFRINLFEDMRLIKDLIFSSKKSGA 234
Query: 257 IILGGSLPKHAIINANLFRGGTDYAIYISTAVPWDGSLSGAPPSEGVSWGKIKAKADYVE 316
+I+GG + KH I N F+ G +YAIYI+TA +DGSLSGA P E +SW KI+ A +V
Sbjct: 235 LIIGGGISKHHTIWWNQFKDGLNYAIYITTAQEYDGSLSGAKPREAISWNKIRPDAKHVT 294
Query: 317 IWADATLVFPIL 328
I+ DAT++ PIL
Sbjct: 295 IYGDATIIVPIL 306
>gi|11499881 deoxyhypusine synthase (dys1-2) [Archaeoglobus fulgidus]
>gi|8928086|sp|O27984|DHY2_ARCFU PROBABLE DEOXYHYPUSINE
SYNTHASE 2 (DHS 2) >gi|7451179|pir||D69537 deoxyhypusine
synthase (EC 1.1.1.249) AF2300 [similarity] -
Archaeoglobus fulgidus >gi|2648217|gb|AAB88953.1|
(AE000945) deoxyhypusine synthase (dys1-2)
[Archaeoglobus fulgidus]
Length = 298
Score = 159 bits (397), Expect = 6e-38
Identities = 102/299 (34%), Positives = 162/299 (54%), Gaps = 30/299 (10%)
Query: 39 KIGFQATHLGKAVKIWRKVEEKRKGGEEVRVFLGYTSNIVSSGLREIIAWLVKERKVDVI 98
+ GFQ LG+A +IW ++ + E + + +G + +V +G+R+IIAWL++ R +DV+
Sbjct: 3 RTGFQGRKLGEAFEIWTEMLRE----ENITILMGLSGAMVPAGMRKIIAWLIRNRYIDVL 58
Query: 99 VTTAGGIEEDFIKTLK-PFILGDWEVNDAELREKGINRIGNIFVPNDRYIEFEKYMVPFF 157
V+T + D + + +G VND +L E+GI+RI ++F +EK
Sbjct: 59 VSTGANLFHDIHEAMGFRHFMGSEHVNDCKLFEEGIDRIHDVFA-------YEKEFNVID 111
Query: 158 ERILDIERKLKRPLTASEFIYEM-GRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGS 216
+ +I ++ +++ EF+ E+ GR L + SI+ AY+ VPIF PAI D S
Sbjct: 112 YTLAEIISEMSGVMSSREFLEEIAGR-----LNVKDRNSIVIAAYESKVPIFSPAIADSS 166
Query: 217 IGDMLYFFKEERHDSKLVIDIANDIVKLNNLAITAKETASIILGGSLPKHAIINANL--- 273
IG K E +VID D+ +L + + +++T I +GG +PK+ I +
Sbjct: 167 IGIAAALAKRE-----VVIDTIRDVEELTEIVVNSEKTGVIYVGGGVPKNFIQQTEVVAR 221
Query: 274 FRG----GTDYAIYISTAVPWDGSLSGAPPSEGVSWGKIKAKADYVEIWADATLVFPIL 328
+G G +YAI I+T VP G LSG EGVSWGKI KA V++ DAT+ P+L
Sbjct: 222 LKGYDVRGHEYAIQITTDVPQFGGLSGCTFEEGVSWGKISGKAKKVQVNCDATIALPVL 280
>gb|AAG01866.1|AF290977_1 (AF290977) deoxyhypusine synthase [Plasmodium falciparum]
Length = 496
Score = 157 bits (393), Expect = 2e-37
Identities = 75/162 (46%), Positives = 114/162 (70%), Gaps = 9/162 (5%)
Query: 171 LTASEFIYEMGRYMDEVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFFKE-ERH 229
L+ SEFI +G+ +++ E S+IYW YK D+PIFCP +TDGS+GD L+ ++
Sbjct: 326 LSPSEFINTLGKEIND------ESSLIYWCYKNDIPIFCPGLTDGSLGDNLFLHNYGKKI 379
Query: 230 DSKLVIDIANDIVKLNNLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYISTAVP 289
+ L++DI DI K+N+LA+ +++ IILGG LPKH + NANL R G D+A+Y++TA
Sbjct: 380 KNNLILDIVKDIKKINSLAMNCEKSGIIILGGGLPKHHVCNANLMRNGADFAVYVNTASE 439
Query: 290 WDGSLSGAPPSEGVSWGKIK--AKADYVEIWADATLVFPILV 329
+DGS SGA +E +SWGKIK ++V+++ DAT++FP++V
Sbjct: 440 YDGSDSGANTTEALSWGKIKYGQTNNHVKVFGDATILFPLMV 481
Score = 110 bits (273), Expect = 2e-23
Identities = 64/193 (33%), Positives = 102/193 (52%), Gaps = 42/193 (21%)
Query: 6 IVLKKSEEIEGLAIEGPWLDEVESLEGVISYYEKIGFQATHLGKAVKIWRKVEEKRKGGE 65
++ +K++ ++GL D+ L I+ Y+ +GFQAT+LG ++ K+ + E
Sbjct: 43 LIPEKTDVVKGLNF-----DKEVDLHEFINNYKYMGFQATNLGISIDEINKMIYYKYKDE 97
Query: 66 EVR-------------------------------------VFLGYTSNIVSSGLREIIAW 88
++ ++L +TSN++SSGLREI +
Sbjct: 98 NIKSEPNNENNLNCNNVSEDLNKDQENHLYHYEKKKKSCIIWLSFTSNMISSGLREIFVY 157
Query: 89 LVKERKVDVIVTTAGGIEEDFIKTLKPFILGDWEVNDAELREKGINRIGNIFVPNDRYIE 148
L K + +DV+VTTAGGIEED IK +GD+ +N +LR+KG NRIGN+ VPND Y +
Sbjct: 158 LAKNKFIDVVVTTAGGIEEDIIKCFSNTYIGDFNLNGKKLRKKGWNRIGNLIVPNDNYCK 217
Query: 149 FEKYMVPFFERIL 161
FE ++ P ++L
Sbjct: 218 FEDWLQPILNKML 230
>sp|O26230|DHYS_METTH PROBABLE DEOXYHYPUSINE SYNTHASE (DHS) >gi|7451178|pir||E69036
deoxyhypusine synthase (EC 1.1.1.249) MTH127
[similarity] - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2621167|gb|AAB84633.1| (AE000802)
deoxyhypusine synthase [Methanobacterium
thermoautotrophicum]
Length = 307
Score = 144 bits (360), Expect = 1e-33
Identities = 79/264 (29%), Positives = 141/264 (52%), Gaps = 13/264 (4%)
Query: 66 EVRVFLGYTSNIVSSGLREIIAWLVKERKVDVIVTTAGGIEEDFIKTLKPFILGDWEVND 125
E+ +F+ +V G+R II L+ + + ++T+ + D ++ D+ +D
Sbjct: 50 EMAIFMSVAGPLVPGGMRRIIRDLIDDGTISALITSGANLTHDLLEAFGGAHYRDYGFDD 109
Query: 126 AELREKGINRIGNIFVPNDRYIEFEKYMVPFFERILDIERKLKRPLTASEFIYEMGRYMD 185
+L ++GI RIG+++ ++ + FE F I K ++ E I+E+G ++D
Sbjct: 110 EKLHQEGIGRIGDVYTRSEDFEVFESENQEIFSSIFSA----KPHISIQELIHEIGGHLD 165
Query: 186 EVLGKEKEKSIIYWAYKRDVPIFCPAITDGSIGDMLYFFKEERHDSKLVIDIANDIVKLN 245
+ + SII A+ R VPI+ P + D +G L+ + + D+++ +D D+ L+
Sbjct: 166 D------DMSIIRTAHLRGVPIYAPGLIDSMLGLQLWMYTQ---DNRICLDAVKDMHSLS 216
Query: 246 NLAITAKETASIILGGSLPKHAIINANLFRGGTDYAIYISTAVPWDGSLSGAPPSEGVSW 305
+L + + +IILGG LPKH + + L RGG D A+ I+ GSLSGAP E SW
Sbjct: 217 DLVFSHERIGAIILGGGLPKHYTLASTLLRGGVDAAVQITMDRSETGSLSGAPLEEAKSW 276
Query: 306 GKIKAKADYVEIWADATLVFPILV 329
K +A ++ V + DAT +FP+++
Sbjct: 277 AKAQAGSNLVTVVGDATALFPVIL 300
>gi|11499777 deoxyhypusine synthase (dys1-1) [Archaeoglobus fulgidus]
>gi|8928087|sp|O28088|DHY1_ARCFU PROBABLE DEOXYHYPUSINE
SYNTHASE 1 (DHS 1) >gi|7451180|pir||C69524 deoxyhypusine
synthase (EC 1.1.1.249) AF2195 [similarity] -
Archaeoglobus fulgidus >gi|2648334|gb|AAB89061.1|
(AE000954) deoxyhypusine synthase (dys1-1)
[Archaeoglobus fulgidus]
Length = 301
Score = 133 bits (332), Expect = 2e-30
Identities = 84/313 (26%), Positives = 157/313 (49%), Gaps = 24/313 (7%)
Query: 17 LAIEGPWLDEVESLEGVISYYEKIGFQATHLGKAVKIWRKVEEKRKGGEEVRVFLGYTSN 76
+ + P + + ++ + F A LG+A KI ++ + + VFL
Sbjct: 1 MRVTSPEIQRGIKVSELLDMFGSTAFNARRLGEAAKICEEMVKS-----DSFVFLTLAGA 55
Query: 77 IVSSGLREIIAWLVKERKVDVIVTTAGGIEEDFIKTLK-PFILGDWEVNDAELREKGINR 135
++ +G+R+I+A +++ + +VTT I + +++L +G V+D L E+ INR
Sbjct: 56 MIPAGMRKIVAGMMQNGFISSLVTTGANIVHEIVESLGIGHEIGSCYVDDTALAEESINR 115
Query: 136 IGNIFVPNDRYIEFEKYMVPFFERILDIERKLKRPLTASEFIYEMGRYMDEVLGKEKEKS 195
I ++FV + + E+++ E + I T EF++E+G+ + + E+S
Sbjct: 116 IYDVFVGQEAFERVEEFLSGIIEGLDGI-------YTTYEFLWEVGKRIPD------ERS 162
Query: 196 IIYWAYKRDVPIFCPAITDGSIGDMLYFFKEERHDSKLVIDIANDIVKLNNLAITAKETA 255
+ A +R++P+FCP + D G + +++ L ID D+ ++ + ++
Sbjct: 163 FLRIAAEREIPVFCPTLHDSIAGLHMTIYRKN-----LQIDFFRDVSRIIDFCFQKRKMG 217
Query: 256 SIILGGSLPKHAIINANLFRGGTDYAIYISTAVPWDGSLSGAPPSEGVSWGKIKAKADYV 315
I++GG +PK+ + A L G DYA+ I+T P G LSGA E SW K+K A V
Sbjct: 218 VIVVGGGVPKNFTLQAMLLAEGFDYAVQITTDSPQWGGLSGATLEEAKSWCKLKPDAKAV 277
Query: 316 EIWADATLVFPIL 328
++ DAT+ P+L
Sbjct: 278 TVYCDATIALPML 290
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.319 0.140 0.414
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 127736095
Number of Sequences: 2977
Number of extensions: 5469816
Number of successful extensions: 12249
Number of sequences better than 1.0e-10: 33
Number of HSP's better than 0.0 without gapping: 32
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 12129
Number of HSP's gapped (non-prelim): 38
length of query: 335
length of database: 189,106,746
effective HSP length: 54
effective length of query: 281
effective length of database: 156,788,448
effective search space: 44057553888
effective search space used: 44057553888
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 165 (68.7 bits)