BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0512 (apbA) DE:PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE (EC
1.1.1.169)
(300 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||H75119 probable 2-dehydropantoate 2-reductase (EC 1.1.1.169... 598 e-170
sp|O50098|APBA_PYRHO PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE (KET... 440 e-123
gi|11499285 thiamine biosynthesis protein (apbA) [Archaeoglobus ... 184 9e-46
gb|AAG19208.1| (AE005017) Vng0730c [Halobacterium sp. NRC-1] 141 1e-32
sp|O67619|APBA_AQUAE PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE (KET... 138 1e-31
emb|CAB56722.1| (AL121600) putative oxidoreductase [Streptomyces... 104 1e-21
pir||B72656 hypothetical protein APE0677 - Aeropyrum pernix (str... 98 2e-19
sp|P77728|APBA_ECOLI 2-DEHYDROPANTOATE 2-REDUCTASE (KETOPANTOATE... 96 4e-19
sp|O34661|APBA_BACSU PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE (KET... 94 3e-18
emb|CAB04765.1| (Z82004) ORF12(1) [Rhodococcus erythropolis] 90 2e-17
pir||D82092 2-dehydropantoate 2-reductase VC2307 [imported] - Vi... 89 6e-17
gi|10956837 unknown [Sphingomonas aromaticivorans] >gi|11361088|... 89 8e-17
pir||E83096 ketopantoate reductase PA4397 [imported] - Pseudomon... 87 2e-16
pir||F69861 thiamin biosynthesis homolog ykpB - Bacillus subtili... 86 4e-16
dbj|BAB06298.1| (AP001516) 2-dehydropantoate 2-reductase [Bacill... 85 1e-15
pir||T35004 probable oxidoreductase - Streptomyces coelicolor >g... 82 8e-15
dbj|BAB05482.1| (AP001513) thiamine biosynthesis [Bacillus halod... 73 5e-12
gi|10956833 unknown [Sphingomonas aromaticivorans] >gi|11361071|... 69 8e-11
>pir||H75119 probable 2-dehydropantoate 2-reductase (EC 1.1.1.169) PAB0512 -
Pyrococcus abyssi (strain Orsay)
>gi|5458184|emb|CAB49673.1| (AJ248285) PROBABLE
2-DEHYDROPANTOATE 2-REDUCTASE (EC 1.1.1.169) [Pyrococcus
abyssi]
Length = 300
Score = 598 bits (1526), Expect = e-170
Identities = 300/300 (100%), Positives = 300/300 (100%)
Query: 1 MKIYILGAGAIGSLFGGLLANAGEDVLLIGRDPHVSAINEKGLKIVGIKDLNVKVEATTR 60
MKIYILGAGAIGSLFGGLLANAGEDVLLIGRDPHVSAINEKGLKIVGIKDLNVKVEATTR
Sbjct: 1 MKIYILGAGAIGSLFGGLLANAGEDVLLIGRDPHVSAINEKGLKIVGIKDLNVKVEATTR 60
Query: 61 VPEEKPDLIVLATKSYSTIEALKSARHIVKGSWVLSIQNGIGNEDKIIEFGGKAIGGITT 120
VPEEKPDLIVLATKSYSTIEALKSARHIVKGSWVLSIQNGIGNEDKIIEFGGKAIGGITT
Sbjct: 61 VPEEKPDLIVLATKSYSTIEALKSARHIVKGSWVLSIQNGIGNEDKIIEFGGKAIGGITT 120
Query: 121 NGAMVEAPGVIKWTGKGVTIIGLYPQGKEKFIEKVADVFNSADIETHVSENIISWIWAKA 180
NGAMVEAPGVIKWTGKGVTIIGLYPQGKEKFIEKVADVFNSADIETHVSENIISWIWAKA
Sbjct: 121 NGAMVEAPGVIKWTGKGVTIIGLYPQGKEKFIEKVADVFNSADIETHVSENIISWIWAKA 180
Query: 181 IVNSAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIEFDVPPMDLFFQTL 240
IVNSAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIEFDVPPMDLFFQTL
Sbjct: 181 IVNSAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIEFDVPPMDLFFQTL 240
Query: 241 EQTRENYNSMLQDIWRGKKTEVDYINGKIVEYAKAVNLEAPMNLLLWGLIKGKEALEGKK 300
EQTRENYNSMLQDIWRGKKTEVDYINGKIVEYAKAVNLEAPMNLLLWGLIKGKEALEGKK
Sbjct: 241 EQTRENYNSMLQDIWRGKKTEVDYINGKIVEYAKAVNLEAPMNLLLWGLIKGKEALEGKK 300
>sp|O50098|APBA_PYRHO PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE (KETOPANTOATE REDUCTASE)
(KPA REDUCTASE) >gi|7521466|pir||H71011 probable thiamin
biosynthesis protein - Pyrococcus horikoshii
>gi|3257813|dbj|BAA30496.1| (AP000006) 301aa long
hypothetical thiamin biosynthesis protein [Pyrococcus
horikoshii]
Length = 301
Score = 440 bits (1120), Expect = e-123
Identities = 203/298 (68%), Positives = 254/298 (85%)
Query: 1 MKIYILGAGAIGSLFGGLLANAGEDVLLIGRDPHVSAINEKGLKIVGIKDLNVKVEATTR 60
MKIYILGAGAIGSL GGLLAN GEDV LIGR H+ AIN++GL I G+ +L + +ATT
Sbjct: 1 MKIYILGAGAIGSLVGGLLANVGEDVTLIGRGRHIEAINKRGLMIEGLTNLKINTKATTS 60
Query: 61 VPEEKPDLIVLATKSYSTIEALKSARHIVKGSWVLSIQNGIGNEDKIIEFGGKAIGGITT 120
+P KPDLI+L TKSYST +AL SA+ IV+ +WVLS+QNGIGNE+KI+E GG+ IGGITT
Sbjct: 61 IPGAKPDLIILTTKSYSTDDALNSAKDIVRDTWVLSLQNGIGNEEKIMELGGRPIGGITT 120
Query: 121 NGAMVEAPGVIKWTGKGVTIIGLYPQGKEKFIEKVADVFNSADIETHVSENIISWIWAKA 180
NGA+++ PGV++W G+G+T+IGLYP+G+ +F+E+V + FN A +ET V+ENII W WAK
Sbjct: 121 NGAVLKEPGVVEWRGRGITLIGLYPKGRNEFVEEVKETFNRAGLETEVTENIIGWKWAKT 180
Query: 181 IVNSAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIEFDVPPMDLFFQTL 240
IVNSAINPIG +LEVKN I++ND+LLS+A+EVVKEGC++ALQNGI+FD+ PM+L QTL
Sbjct: 181 IVNSAINPIGAILEVKNGAIKDNDYLLSIAVEVVKEGCKIALQNGIKFDISPMELLIQTL 240
Query: 241 EQTRENYNSMLQDIWRGKKTEVDYINGKIVEYAKAVNLEAPMNLLLWGLIKGKEALEG 298
EQTRENYNSMLQDIWRGK+TE+D+INGKI+EYAK VNLEAP+N LLW L+K KE+L G
Sbjct: 241 EQTRENYNSMLQDIWRGKRTEIDFINGKIIEYAKLVNLEAPLNFLLWALVKAKESLGG 298
>gi|11499285 thiamine biosynthesis protein (apbA) [Archaeoglobus fulgidus]
>gi|3913072|sp|O28578|APBA_ARCFU PROBABLE
2-DEHYDROPANTOATE 2-REDUCTASE (KETOPANTOATE REDUCTASE)
(KPA REDUCTASE) >gi|7483975|pir||F69461 thiamin
biosynthesis protein (apbA) homolog - Archaeoglobus
fulgidus >gi|2648858|gb|AAB89553.1| (AE000986) thiamine
biosynthesis protein (apbA) [Archaeoglobus fulgidus]
Length = 294
Score = 184 bits (463), Expect = 9e-46
Identities = 108/294 (36%), Positives = 167/294 (56%), Gaps = 6/294 (2%)
Query: 2 KIYILGAGAIGSLFGGLLANAGEDVLLIGRDPHVSAINEKGLKIVGIKDLNVKVEATTRV 61
++ I+GAGA+GSL G L+ AG DV+ + R + A+ +KGL++ G+K+ +KV T++
Sbjct: 5 RVQIMGAGALGSLVGALIQLAGYDVIFVARGKQLEAL-KKGLRVSGLKNAELKVYCTSQ- 62
Query: 62 PEEKPDLIVLATKSYSTIEALKSARHIVKGSWVLSIQNGIGNEDKIIEFGGKAIGGITTN 121
E D+ + K+Y T K + G V S+QNG+GNE+ + ++ K +GG+TT
Sbjct: 63 -PEDADITFVTVKAYDTETVAKKLAEVDAGV-VCSLQNGVGNEEILAKYCRKVLGGVTTY 120
Query: 122 GAMVEAPGVIKWTGKGVTIIGLYPQGKEKFIEKVADVFNSADIETHVSENIISWIWAKAI 181
GA ++ G + + G+G T +G E VA+V A + +I IWAKA+
Sbjct: 121 GANLKDYGHVVYAGEGYTYVGEMDGRVSGEAEMVAEVLRDAGMRAEAVNDIEFRIWAKAV 180
Query: 182 VNSAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIEFDVPPMDLFFQTLE 241
VN+AINPI + VKN + N L +A V EG +V + G EFD + E
Sbjct: 181 VNAAINPITAICRVKNGEVVRNPHLWEVARAVADEGRQVMARMGYEFDA--ASEVRKVAE 238
Query: 242 QTRENYNSMLQDIWRGKKTEVDYINGKIVEYAKAVNLEAPMNLLLWGLIKGKEA 295
T EN +SMLQD+ RGK+TEV++ING IV+ + ++ +N L L++G E+
Sbjct: 239 MTAENRSSMLQDLERGKRTEVEFINGAIVKKGEEFGIDCAVNRTLLNLVRGVES 292
>gb|AAG19208.1| (AE005017) Vng0730c [Halobacterium sp. NRC-1]
Length = 303
Score = 141 bits (351), Expect = 1e-32
Identities = 94/304 (30%), Positives = 151/304 (48%), Gaps = 20/304 (6%)
Query: 1 MKIYILGAGAIGSLFGGLLANAGEDVLLIG-RDPHVSAINEKGLKIVGIKDLNVKVEATT 59
M++ + G G++GSL GG+LA V L+G + H++ + E GL++V + + TT
Sbjct: 1 MRVVVQGPGSLGSLVGGVLAGGETAVTLLGHQSEHLTRVREDGLRVV-------QPDGTT 53
Query: 60 RVPEEK----------PDLIVLATKSYSTIEALKSARHIVKGSWVLSIQNGIGNEDKIIE 109
RV DL+V+ KSY T A ++ G+ VL++QNG+GN + E
Sbjct: 54 RVTRPSVATDPSVVADADLVVVCVKSYDTASAARALGRQCDGAMVLTLQNGLGNAAVLAE 113
Query: 110 F--GGKAIGGITTNGAMVEAPGVIKWTGKGVTIIGLYPQGKEKFIEKVADVFNSADIETH 167
+ G TT+GA PGV++ G G T IG Y + + VA F++ +ET
Sbjct: 114 HVPADTVLVGTTTHGAARTEPGVVRHAGGGETTIGRYRGANDARVASVAAAFSTGGMETT 173
Query: 168 VSENIISWIWAKAIVNSAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIE 227
V+ + +W K +VN IN L +V N + E + V EG RVA G+
Sbjct: 174 VTASPQRAVWEKVLVNVGINAATALADVDNGALVECPPGERVLERAVTEGVRVAEAEGVS 233
Query: 228 FDVPPMDLFFQTLEQTRENYNSMLQDIWRGKKTEVDYINGKIVEYAKAVNLEAPMNLLLW 287
++ Q +T N +SM QD+ G +TEV+ ++G +VE A+ ++ P+ L
Sbjct: 234 VSESVVERARQVAARTASNESSMRQDLAGGARTEVESLHGAVVERARDHDIAVPVIRTLA 293
Query: 288 GLIK 291
L++
Sbjct: 294 DLVR 297
>sp|O67619|APBA_AQUAE PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE (KETOPANTOATE REDUCTASE)
(KPA REDUCTASE) >gi|7517478|pir||A70449 hypothetical
protein aq_1727 - Aquifex aeolicus
>gi|2984043|gb|AAC07585.1| (AE000753) putative protein
[Aquifex aeolicus]
Length = 310
Score = 138 bits (343), Expect = 1e-31
Identities = 101/308 (32%), Positives = 161/308 (51%), Gaps = 21/308 (6%)
Query: 1 MKIYILGAGAIGSLFGGLLANAGEDVL-LIGRDPHVSAINEKGLKIVGI-KDLNVKVEAT 58
MK I+G GAIGS + L AG + L+ R+P +N +K+ GI + + V+
Sbjct: 3 MKFLIVGVGAIGSAYLAFLTRAGHEAAGLVRRNP----VNR--IKVEGIWGEFEIPVKTF 56
Query: 59 TRVPEEK--PDLIVLATKSYSTIEALKSARHIV-KGSWVLSIQNGIGNEDKIIEF--GGK 113
T+V E PD+++++ KSY T EALK + +V + ++++ QNG GN +K +E GK
Sbjct: 57 TKVEEVPFIPDIVIISVKSYDTEEALKKVKPVVGENTFIMIAQNGYGNYEKAVEIYGEGK 116
Query: 114 AIGGITTNGAMVEAPGVIKWTGKGVTIIGLYPQGK--EKFIEKVADVFNSADIETHVSEN 171
I G+ V PG I+ T ++ P GK E+F++ +A F A I T +
Sbjct: 117 VILSRIIFGSKVIKPGHIRITVSADEVVIGDPSGKIDEEFLKNLARTFTEAGIPTRYERD 176
Query: 172 IISWIWAKAIVNSAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIEFDVP 231
+ ++ K I NSA+NP+G L EV + EN + V+ E +V + +
Sbjct: 177 VYKYLVDKIIYNSALNPLGALFEVNYGSLAENPHTKELMNRVIDEIFQVIEKAKLPCFWK 236
Query: 232 PMD----LFFQTL-EQTRENYNSMLQDIWRGKKTEVDYINGKIVEYAKAVNLEAPMNLLL 286
D +F++ L T E+Y SML+D+ +G KTE++ +NG IVE K + P N +
Sbjct: 237 SADEYKKVFYEKLIPPTAEHYPSMLEDVKKG-KTEIEALNGAIVELGKKYGVSTPTNEFI 295
Query: 287 WGLIKGKE 294
++K KE
Sbjct: 296 TKMVKAKE 303
>emb|CAB56722.1| (AL121600) putative oxidoreductase [Streptomyces coelicolor A3(2)]
Length = 309
Score = 104 bits (257), Expect = 1e-21
Identities = 78/293 (26%), Positives = 136/293 (45%), Gaps = 12/293 (4%)
Query: 1 MKIYILGAGAIGSLFGGLLANAGEDVLLIGRDPHVSAINEKGLKIVG-IKDLNVKVEATT 59
M+I ++GAG +G FG LA AG +V + R H++AI GL + + +L ++
Sbjct: 1 MRIAVIGAGGVGGYFGARLAAAGNEVTFVARGGHLAAIRRSGLVVHSPLGELRTSPDSVV 60
Query: 60 RVPEE--KPDLIVLATKSYSTIEALKSAR-HIVKGSWVLSIQNGIGNEDKIIE--FGGKA 114
E DL+++A K + T + R G+ VLS+QNG+ +D + +
Sbjct: 61 ASISELGPTDLVLVAVKLWDTQAVAEELRTSPAHGAPVLSLQNGVHKDDVLRGRLTDEQV 120
Query: 115 IGGITTNGAMVEAPGVIKWTGK-GVTIIGLYPQGKEKFIEKVADVFNSADIETHVSENII 173
+GG+ A +E PGV++ G ++G Y G + V F A I+ S++I
Sbjct: 121 LGGVCFISAFIEEPGVVRHNGPLQKVVLGPYRSGSTATVRAVLAAFRDAGIDAEGSDDIE 180
Query: 174 SWIWAKAIVNSAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIEFD---V 230
IW K + ++ + V+R N+ + EV+ E VA +G+ D
Sbjct: 181 RAIWEKFVFLVGLSATTATVRQPVGVVRANERSRRLLYEVMAETVAVAQASGVRLDSGFA 240
Query: 231 PPMDLFFQTLEQTRENYNSMLQDIWRGKKTEVDYINGKIVEYAKAVNLEAPMN 283
F TL +SM D+ +G + E+ +++G +V+ A +++ P N
Sbjct: 241 EDRLAFCDTLPAAMT--SSMHNDLEKGNRLELPWLSGGVVDLADRLDVPVPRN 291
>pir||B72656 hypothetical protein APE0677 - Aeropyrum pernix (strain K1)
>gi|5104335|dbj|BAA79650.1| (AP000060) 320aa long
hypothetical protein [Aeropyrum pernix]
Length = 320
Score = 97.5 bits (239), Expect = 2e-19
Identities = 76/294 (25%), Positives = 130/294 (43%), Gaps = 4/294 (1%)
Query: 5 ILGAGAIGSLFGGLL-ANAGEDVLLIGRDPHVSAINEKGLKIVGIKDLNVKVEATTRVPE 63
I+G G +GSL + G+ + + R + + GL++ G+ D V +E P
Sbjct: 17 IVGLGGVGSLLAYYVYRGTGDTPIAVVRRRSQAMRLQAGLRLYGVVDAYVPLEPYVGTPP 76
Query: 64 EKPDLIVLATKSYSTIEALKSARHIVKGSWVLSIQNGIGNEDKIIEFGGKAIGGITTNGA 123
L ++ K+Y A+++A V NG G +K ++ G A + +G
Sbjct: 77 RGCRLSFISVKAYDAAAAVEAAAEF--SEMVAVAGNGWGGYEKALDLGVDAAAIVVESGV 134
Query: 124 MVEAPGVIKWTGKGVTIIGLYPQGKEKFIEKVADVFNSADIETHVSENIISWIWAKAIVN 183
PG+ + G G I+G E ++ ++ +I +W W KA N
Sbjct: 135 ASLEPGLARVAGLGRLIVGSRGGVSGAAFESF-EILRRGGAPALLAGDIEAWRWLKAAAN 193
Query: 184 SAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIEFDVPPMDLFFQTLEQT 243
SAIN + T+L +N + E+ +A E VA G P++ QT
Sbjct: 194 SAINLVTTILGSRNGFVVESREAWRLASRAASEVGLVASMLGYSLPEDPVEYTRLVALQT 253
Query: 244 RENYNSMLQDIWRGKKTEVDYINGKIVEYAKAVNLEAPMNLLLWGLIKGKEALE 297
+N +S LQD+ R +++E YI G++VE A + + AP L+ L++ AL+
Sbjct: 254 YDNESSTLQDLRRCRRSEAPYIAGRVVEEASRLGVGAPTVEALYSLLQALFALK 307
>sp|P77728|APBA_ECOLI 2-DEHYDROPANTOATE 2-REDUCTASE (KETOPANTOATE REDUCTASE) (KPA
REDUCTASE) >gi|7466605|pir||A64772 ketopantoate
reductase - Escherichia coli >gi|1773109|gb|AAB40181.1|
(U82664) similar to S. typhimurium apbA [Escherichia
coli] >gi|1786627|gb|AAC73528.1| (AE000148) involved in
thiamin biosynthesis, alternative pyrimidine
biosynthesis [Escherichia coli K12]
Length = 303
Score = 96.3 bits (236), Expect = 4e-19
Identities = 83/299 (27%), Positives = 137/299 (45%), Gaps = 13/299 (4%)
Query: 1 MKIYILGAGAIGSLFGGLLANAGEDVLLIGRDPHVSAINEKGLKIVGIKDLNVKVEATTR 60
MKI +LG GA+G L+ L G +V R P + +V T
Sbjct: 1 MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYC----SVNLVETDGSIFNESLTAN 56
Query: 61 VPE--EKPDLIVLATKSYSTIEALKS-ARHIVKGSWVLSIQNGIGNEDKIIEFGGKAIGG 117
P+ DL+++ K++ +A+KS A + + +L I NG+G +++ + G
Sbjct: 57 DPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGTIEELQNIQQPLLMG 116
Query: 118 ITTNGAMVEAPGVIKWTGKGVTIIGLYPQGKEKFIEKVADVFNSADIETHVSENIISWIW 177
TT+ A + VI G+T IG Q + +AD+ + + NI + +W
Sbjct: 117 TTTHAARRDG-NVIIHVANGITHIGPARQQDGDY-SYLADILQTVLPDVAWHNNIRAELW 174
Query: 178 AKAIVNSAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIEFDVPPM-DLF 236
K VN INP+ + N +R + M++ +E V + G + D
Sbjct: 175 RKLAVNCVINPLTAIWNCPNGELRHHP---QEIMQICEEVAAVIEREGHHTSAEDLRDYV 231
Query: 237 FQTLEQTRENYNSMLQDIWRGKKTEVDYINGKIVEYAKAVNLEAPMNLLLWGLIKGKEA 295
Q ++ T EN +SMLQDI + TE+DYING ++ A+A + P N L+ ++K KE+
Sbjct: 232 MQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVPENTRLFEMVKRKES 290
>sp|O34661|APBA_BACSU PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE (KETOPANTOATE REDUCTASE)
(KPA REDUCTASE) >gi|7475787|pir||F69875
pyrimidine-thiamin biosynthesis homolog ylbQ - Bacillus
subtilis >gi|2340014|emb|CAB11364.1| (Z98682) YlbQ
protein [Bacillus subtilis] >gi|2633882|emb|CAB13384.1|
(Z99111) similar to pyrimidine-thiamine biosynthesis
[Bacillus subtilis]
Length = 298
Score = 93.6 bits (229), Expect = 3e-18
Identities = 78/297 (26%), Positives = 138/297 (46%), Gaps = 9/297 (3%)
Query: 1 MKIYILGAGAIGSLFGGLLANAGEDVLLIGRDPHVSAINEKGLKIV-GIKDLNVKVEATT 59
MKI I+G G++G L L+ + ++ R +AI +G+++ G ++ A T
Sbjct: 1 MKIGIIGGGSVGLLCAYYLSLYHDVTVVTRRQEQAAAIQSEGIRLYKGGEEFRADCSADT 60
Query: 60 RVPEEKPDLIVLATKSYSTIEALKSARHIVKGSWVLSIQNGIGNEDKIIEF--GGKAIGG 117
+ + DL+V+ K + S I K + +L +QNG+G+ + ++ G G
Sbjct: 61 SINSDF-DLLVVTVKQHQLQSVFSSLERIGKTN-ILFLQNGMGHIHDLKDWHVGHSIYVG 118
Query: 118 ITTNGAMVEAPGVIKWTGKGVTIIGLYPQGKEKFIEKVADVFNSADIETHVSENIISWIW 177
I +GA+ ++ + TG G + + + + N +D + + +
Sbjct: 119 IVEHGAVRKSDTAVDHTGLGAIKWSAFDDAEPDRLN-ILFQHNHSDFPIYYETDWYRLLT 177
Query: 178 AKAIVNSAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIEFDVPPMDLFF 237
K IVN+ INP+ LL+VKN + L+ V +E CR+ +E + +
Sbjct: 178 GKLIVNACINPLTALLQVKNGELLTTPAYLAFMKLVFQEACRILK---LENEEKAWERVQ 234
Query: 238 QTLEQTRENYNSMLQDIWRGKKTEVDYINGKIVEYAKAVNLEAPMNLLLWGLIKGKE 294
QT+EN +SML D+ G++TE D I G +++ A L+A L+G IK E
Sbjct: 235 AVCGQTKENRSSMLVDVIGGRQTEADAIIGYLLKEASLQGLDAVHLEFLYGSIKALE 291
>emb|CAB04765.1| (Z82004) ORF12(1) [Rhodococcus erythropolis]
Length = 367
Score = 90.5 bits (221), Expect = 2e-17
Identities = 96/330 (29%), Positives = 151/330 (45%), Gaps = 49/330 (14%)
Query: 1 MKIYILGAGAIGSLFGGLLANAGEDVLLIGRDPHVSAINEKGLKIVGIKDLNVKVEATTR 60
++I + GAG+IG GG LA G +V LIGR+ + I + GL++ + +++V A+
Sbjct: 8 LRISVFGAGSIGCYLGGRLAATGAEVTLIGRERVGAEIRDHGLRLTDLHGSDIRVAASDI 67
Query: 61 VPEEKP------DLIVLATKSYSTIE-ALKSARHIVKGSWVLSIQNGIGNEDKIIEFGGK 113
+ P DL+V++ KS T E A I G+ V+S QNG+GN D +
Sbjct: 68 AFDTGPGSAATADLVVVSVKSAGTPEVGATLASAIGPGTVVVSAQNGLGNADTL----RA 123
Query: 114 AIGGITTNGAMVEAPGVIKWTGKGVTIIGLYPQGKEKFIE--------KVADVFNSADIE 165
A+ G AMV V + G + QG E +E A VF A +
Sbjct: 124 ALPGHVVLAAMVPFNVVHRGDGH-------FHQGSEGDLEIEQHPAVPAFAPVFADAGLP 176
Query: 166 THVSENIISWIWAKAIVNSAINPIGTL--LEVKNKVIRENDFLLSMAMEVVKEGCRVALQ 223
H ++ S WAK ++N NP+ L L ++ ++ R F ++A+ +E +
Sbjct: 177 LHPHDDFRSVQWAKLLLN-LNNPVNALSGLPLREELSR-RPFRRALAL-AQREALALLAA 233
Query: 224 NGIEF----DVPPM---------DLFFQT-----LEQTRENYNSMLQDIWRGKKTEVDYI 265
GI +PP D F+ L +SM D+ ++TEVD+I
Sbjct: 234 RGIAVAKLTPLPPQWIPRLLTVPDGVFRVAGSRMLAIDPHARSSMWDDLEACRRTEVDWI 293
Query: 266 NGKIVEYAKAVNLEAPMNLLLWGLIKGKEA 295
+G+I+ A+A AP+N L LI+ EA
Sbjct: 294 SGEIMRLAEASGTAAPVNARLVALIREAEA 323
>pir||D82092 2-dehydropantoate 2-reductase VC2307 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9656872|gb|AAF95451.1|
(AE004301) 2-dehydropantoate 2-reductase [Vibrio
cholerae]
Length = 296
Score = 88.9 bits (217), Expect = 6e-17
Identities = 79/301 (26%), Positives = 139/301 (45%), Gaps = 18/301 (5%)
Query: 1 MKIYILGAGAIGSLFGGLLANAGEDVLLIGRDPHVSA---INEKGLKIVGIKDLNVKVEA 57
M I +LG GA+GSL+ L +AG V L R + ++E+ ++L+ + A
Sbjct: 1 MNIVVLGPGAVGSLWALHLHSAGHQVALWSRQAQPTITLQLDEEAPISFRNQNLDTLIHA 60
Query: 58 TTRVPEEKPDLIVLATKSYSTIEALKSAR-HIVKGSWVLSIQNGIGNEDKIIEF--GGKA 114
DL+++ K++ AL+ H+ + + +L + NG+G + I E
Sbjct: 61 ---------DLLLITVKAWQVEAALQPLLPHLNRETILLFMHNGMGAVEAISESLTHFPV 111
Query: 115 IGGITTNGAMVEAPGVIKWTGKGVTIIGLYPQGKEKFIEKVADVFNSADIETHVSENIIS 174
+ TT+GA+ + TG G T +G + + + +ADVFN A + I
Sbjct: 112 LFATTTHGALKATLNQVSHTGFGQTQVGPFNALGAR-CDFIADVFNHALAPVTWNPEIQQ 170
Query: 175 WIWAKAIVNSAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIEFDVPPM- 233
+W K VN AINP+ + + N + +F + ++ E V I + +
Sbjct: 171 ALWRKLAVNCAINPLTAIHQCANGALVAPEFT-PIITAILDEVTAVMQAEAISGEAEALR 229
Query: 234 DLFFQTLEQTRENYNSMLQDIWRGKKTEVDYINGKIVEYAKAVNLEAPMNLLLWGLIKGK 293
D +Q ++ T N +SM QD++ + TE+D+I G +V + + P+N L+ IK
Sbjct: 230 DGVYQVIQATAANLSSMHQDVFHRRPTEIDFITGYVVRKGEQHGIATPVNSALYQQIKTL 289
Query: 294 E 294
E
Sbjct: 290 E 290
>gi|10956837 unknown [Sphingomonas aromaticivorans] >gi|11361088|pir||T31129
hypothetical protein 58 - Sphingomonas aromaticivorans
plasmid pNL1 >gi|3378270|gb|AAD03853.1| (AF079317)
unknown [Sphingomonas aromaticivorans]
Length = 337
Score = 88.5 bits (216), Expect = 8e-17
Identities = 92/328 (28%), Positives = 144/328 (43%), Gaps = 50/328 (15%)
Query: 7 GAGAIGSLFGGLLANAGEDVLLIGRDPHVSAINEKGLKIVGIKDLNVKVE--ATTRVPEE 64
GAG +GS+ GG LA G V LI R PHV AI GL I G++ V+ E PEE
Sbjct: 11 GAGGLGSVIGGFLARGGHKVTLIARKPHVEAIRRDGLSISGVRAQFVQRENLFAVETPEE 70
Query: 65 KP---DLIVLATKSYSTIEALKSARHIV-KGSWVLSIQNGIGNEDKI-IEFG-GKAIGGI 118
D +L TK+ T +AL A+ +V + + L++QNG+G E K+ FG K IGG
Sbjct: 71 VEGDIDYYILLTKAKGTDQALADAQVLVDRTACALTLQNGVGKEGKLQAAFGKDKVIGGS 130
Query: 119 TTNGAMVEAPG-VIKWTGKGVT-IIGLYPQGKEKFIEKVADVFNSADIETHVSENIISWI 176
+GA + PG + VT G G+ + +A+ ++A + + + +I+
Sbjct: 131 IMDGATLLEPGKALNHMAVPVTAYFGELEGGESERTRIMAEALDAAGMGSRSTPDIVHVH 190
Query: 177 WAKAI--------VNSAINPIGTLLEVKNKVIRE---------NDFL------------- 206
W K + S + + L V +RE D L
Sbjct: 191 WEKVVQVGGASSWSASTLGALPKLDFVDGVAVREGAEHYVHVVKDLLAIYKALGYEPQNY 250
Query: 207 ---LSMAMEVVKEGCRVALQNGIEFDVPPMDLFFQTLEQTRENYNSMLQDIWRGKKTEVD 263
+S +E+ +EG A+ V M F+ + R SM D+ G++ EVD
Sbjct: 251 FAPVSRLVEIDREGFEEAVSG-----VMAMGTRFK--PENRPVRTSMHDDLVAGRRMEVD 303
Query: 264 YINGKIVEYAKAVNLEAPMNLLLWGLIK 291
+ G + A+ + + AP L + ++K
Sbjct: 304 EVLGPLAAAAEQLQVPAPAFLSAYRVLK 331
>pir||E83096 ketopantoate reductase PA4397 [imported] - Pseudomonas aeruginosa
(strain PAO1) >gi|9950628|gb|AAG07785.1|AE004855_7
(AE004855) ketopantoate reductase [Pseudomonas
aeruginosa]
Length = 303
Score = 87.4 bits (213), Expect = 2e-16
Identities = 75/290 (25%), Positives = 138/290 (46%), Gaps = 17/290 (5%)
Query: 1 MKIYILGAGAIGSLFGGLLANAGEDVLLIGRDPHVSAINEK--GLKIVGIKDLNV-KVEA 57
M +ILGAG++GSL+ L AG V LI RD ++ GL +V ++ + A
Sbjct: 1 MTWHILGAGSLGSLWAARLGRAGLPVRLILRDRQRLRRYQQAGGLSLVEDGQASLYPIAA 60
Query: 58 TTRVPEEKPDLIVLATKSYSTIEALKSARHIVKGSW-VLSIQNGIGNEDKIIEF--GGKA 114
T + ++LA K+Y EA S H + G+ +L +QNG+G++ + +
Sbjct: 61 ETPDGGQPIQRLLLACKAYDAEEAASSVAHRLAGNAELLLLQNGLGSQQAVAARLPRSRC 120
Query: 115 IGGITTNGAMVEAPGVIKWTGKGVTIIGLYPQGKEKFIEKVADVFNSADIETHVSENIIS 174
+ +T GA + + + G+G T +G ++ + A I S++I+
Sbjct: 121 LFASSTEGAFRDGDFRVVFAGRGHTWLG---DPRDTNAPAWLTQLSQAGIPHSWSDDILE 177
Query: 175 WIWAKAIVNSAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIEFDVPPMD 234
+W K +N AINP+ L + +N +R++ ++ + + + L + +D
Sbjct: 178 RLWRKLALNCAINPLTVLHDCRNGGLRQHPEEIAALCDELGQ-----LLHASGYDAAARS 232
Query: 235 LFFQT---LEQTRENYNSMLQDIWRGKKTEVDYINGKIVEYAKAVNLEAP 281
L ++ T NY+SM QD+ RG++TE+ Y+ G ++ + + L P
Sbjct: 233 LLEDVRAVIDATAANYSSMHQDVTRGRRTEIGYLLGYACQHGQRLGLPLP 282
>pir||F69861 thiamin biosynthesis homolog ykpB - Bacillus subtilis
>gi|2633815|emb|CAB13317.1| (Z99111) similar to thiamin
biosynthesis [Bacillus subtilis]
>gi|3282129|gb|AAC24919.1| (AF012285) YkpB [Bacillus
subtilis]
Length = 303
Score = 86.2 bits (210), Expect = 4e-16
Identities = 69/288 (23%), Positives = 138/288 (46%), Gaps = 6/288 (2%)
Query: 1 MKIYILGAGAIGSLFGGLLANAGEDVLLIGRDPHVSAINEKGLKIVGIK-DLNVKVEATT 59
MK ++GAG +G GG L+ G DV + R + + GL I K +++ + E +
Sbjct: 1 MKFLVVGAGGVGGYIGGRLSEKGNDVTFLVRQKRAEQLKKTGLVIHSEKGNVSFQPELIS 60
Query: 60 RVPEEKPDLIVLATKSYSTIEALKSARHIV-KGSWVLSIQNGIGNEDKIIEFGGK--AIG 116
+ D++++A+K+YS + ++ + + + S ++ NG + +++ K +G
Sbjct: 61 AGETGQFDVVIIASKAYSLGQVIEDVKPFIHQESVIIPFLNGYRHYEQLFAAFSKEQVLG 120
Query: 117 GITTNGAMVEAPGVIKWTGKGVT-IIGLYPQGKEKFIEKVADVFNSADIETHVSENIISW 175
G+ + ++ G I T + G + + + I + + F+ E +S +I
Sbjct: 121 GLCFIESALDNKGEIHHTSASHRFVFGEWNGERTERIRALEEAFSGVKAEVIISGHIEKD 180
Query: 176 IWAKAIVNSAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIEFDVPPMDL 235
IW K + +A I TL + I + A ++ E C V + G+ D +
Sbjct: 181 IWKKYLFIAAQAGITTLFQRPLGPILATEAGRHTAQTLIGEICTVLRKEGVPADPALEEE 240
Query: 236 FFQTL-EQTRENYNSMLQDIWRGKKTEVDYINGKIVEYAKAVNLEAPM 282
F+T+ + +SML+D+ G+ TE D+++G ++E AK ++L AP+
Sbjct: 241 SFRTMTSMSYHMKSSMLRDMENGQTTEGDHLHGFLIEKAKRLSLAAPV 288
>dbj|BAB06298.1| (AP001516) 2-dehydropantoate 2-reductase [Bacillus halodurans]
Length = 308
Score = 84.7 bits (206), Expect = 1e-15
Identities = 77/306 (25%), Positives = 144/306 (46%), Gaps = 13/306 (4%)
Query: 2 KIYILGAGAIGSLFGGLLANAGEDVLLIGRDPH-VSAINEKGLKIVGI----KDLNVKVE 56
++ ++G+G+IG L L A LL+ P +N++GL V + ++V
Sbjct: 5 RVAVIGSGSIGLLLYAYLKLANWPALLVTNRPEQADHLNKEGLTFVAKSGEHQPISVTAT 64
Query: 57 ATTRVPEEKPDLIVLATKSYSTIEALKSARHIVKGSWVLSIQNGIGNEDKIIEFGGKAIG 116
+ E+ ++ + KS++ ++AL K ++ QNG+ + + + + +AI
Sbjct: 65 PLSSFSAEEAEVGFITVKSFA-VKALLPQLQRKKWDGLVFFQNGMTHLEILRQIPARAIC 123
Query: 117 -GITTNGAMVEAPGVIKWTGKGVTIIGLYPQGKEKFIEKVADVFNSADIETHVSENIISW 175
GI GA E+ ++ TG G IG G K + + ++A ++ N
Sbjct: 124 CGIVEIGAKRESDVRVQHTGDGAIWIGDMV-GDSKNFDPMWACLHNAGLKAQKVTNWQDK 182
Query: 176 IWAKAIVNSAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIEFDVPPMDL 235
+ K I N+AINP+ L VKN + EN + E+VKE C + G E +V
Sbjct: 183 MERKLIANAAINPLTGLFRVKNGALIENQLFSLLLKELVKEAC---VTLGREREVDEHLA 239
Query: 236 FFQTL-EQTRENYNSMLQDIWRGKKTEVDYINGKIVEYAKAVNLEAPMNLLLWGLIKGKE 294
+ + + ++T N +SM D+ ++TE++ I G +++ A +E P ++ + G +
Sbjct: 240 YTRAICDKTANNLSSMYMDLTHHRRTEIESITGYLIKEAAKKGIEIPNTSFVYHAVMGMD 299
Query: 295 ALEGKK 300
EG+K
Sbjct: 300 E-EGRK 304
>pir||T35004 probable oxidoreductase - Streptomyces coelicolor
>gi|4007680|emb|CAA22366.1| (AL034443) putative
oxidoreductase [Streptomyces coelicolor A3(2)]
Length = 323
Score = 81.9 bits (199), Expect = 8e-15
Identities = 79/311 (25%), Positives = 135/311 (43%), Gaps = 36/311 (11%)
Query: 1 MKIYILGAGAIGSLFGGLLANAGEDVLLIGRDPHVSAINEKGLKIVGIK-DLNVKVEATT 59
MK+ +LGAGAIG+ G L AG DV LI R PH++A+ + G+++ + D AT
Sbjct: 1 MKVAVLGAGAIGAYVGAALHRAGADVHLIARGPHLAAMRQYGVRVRSPRGDFTAHPRATD 60
Query: 60 RVPEEKP-DLIVLATKSYS------TIEALKSARHIVKGSWVLSIQNGI----------- 101
E P D + L K+ S IE L + + + V++ QNGI
Sbjct: 61 DPAEVGPVDYVFLGLKANSYAACGPLIEPL-----LHRTTAVVAAQNGIPWWYFHRHGGP 115
Query: 102 --GNEDKIIEFGG---------KAIGGITTNGAMVEAPGVIKWTGKGVTIIGLYPQGKEK 150
G + ++ G +A+G + +E PGV++ IG +
Sbjct: 116 YDGRRLESVDPAGAVSAVLAPERAVGCVVYAATELEQPGVVRHVEGTRFSIGEPGREVSP 175
Query: 151 FIEKVADVFNSADIETHVSENIISWIWAKAIVNSAINPIGTLLEVKNKVIRENDFLLSMA 210
+++ S ++ V ++ + IW K + N + NPI L+ + + + +
Sbjct: 176 RCLALSEAMQSGGLKCPVEPDLRNDIWLKLLGNISFNPISALVRATMRQMCRHGGTREVI 235
Query: 211 MEVVKEGCRVALQNGIEFDVPPMDLFFQTLEQTRENYNSMLQDIWRGKKTEVDYINGKIV 270
++ E VA G E V ++ E+ ++ S LQD+ RGK E+D + +V
Sbjct: 236 ETMMTETLAVAAALGCEIGV-SIERRLAGAERVGDHRTSTLQDLERGKPLELDVLLAAVV 294
Query: 271 EYAKAVNLEAP 281
E A+ + P
Sbjct: 295 ELAEITGVPVP 305
>dbj|BAB05482.1| (AP001513) thiamine biosynthesis [Bacillus halodurans]
Length = 304
Score = 72.6 bits (175), Expect = 5e-12
Identities = 69/307 (22%), Positives = 143/307 (46%), Gaps = 44/307 (14%)
Query: 1 MKIYILGAGAIGSLFGGLLANAGEDVLLIGRDPHVSAINEKGLKIVGIKDLNVKVEATTR 60
MK I+GAGA+G FG LA GEDV + R+ + ++GL+I I N ++A T
Sbjct: 1 MKFLIVGAGAVGGYFGARLAEKGEDVTFLVREKRKEQMKKRGLRIQSIHG-NTTIQAKTI 59
Query: 61 VPEEKP--DLIVLATKSYSTIEALKSARHIVKGSWV-LSIQNGIGNEDKIIEFGG--KAI 115
E+ D+++L TK+Y +++ R + V + + NGI + D++ + + I
Sbjct: 60 TMEDTGLFDVVLLGTKAYHLENVIETIRPFIHDQTVIIPMLNGIAHLDRLRKAFSTEQVI 119
Query: 116 GGIT-TNGAMVEAPGVIKWTGKGVTIIGLYPQGKEKFIEKVADVFNSADIETHVSENIIS 174
GG+ + E +++ + + G + + ++K+ F + VS+++++
Sbjct: 120 GGLCFIESTLNEFGDIVQTSQIHRFVFGELNGERSERVKKIEAAFAGTNAMIEVSDSVLT 179
Query: 175 WIWAKAIVNSAIN--------PIGTLLEVKN--KVIRENDFLLSMAMEVVKEGCRVALQ- 223
+W K + + ++ P+G + E+ + +VIR + M V G +A Q
Sbjct: 180 EMWHKYMFITTMSGVTTLYNQPVGPIRELPSGEQVIRGLFAEIGAIMRAV--GAPIADQV 237
Query: 224 --------NGIEFDVPPMDLFFQTLEQTRENYNSMLQDIWRGKKTEVDYINGKIVEYAKA 275
G+E+++ +SM +D+ +G E +++ G +++ A+
Sbjct: 238 EEKQWKQMGGVEYEMK----------------SSMQRDMEKGLPIEREHLQGYLLKLAEL 281
Query: 276 VNLEAPM 282
++AP+
Sbjct: 282 HQIQAPI 288
>gi|10956833 unknown [Sphingomonas aromaticivorans] >gi|11361071|pir||T31125
hypothetical protein 29 - Sphingomonas aromaticivorans
plasmid pNL1 >gi|3378266|gb|AAD03849.1| (AF079317)
unknown [Sphingomonas aromaticivorans]
Length = 310
Score = 68.7 bits (165), Expect = 8e-11
Identities = 69/293 (23%), Positives = 133/293 (44%), Gaps = 15/293 (5%)
Query: 2 KIYILGAGAIGSLFGGLLANAGEDVLLIGRDPHVSAINEKGLKI-VGIKDLNVKVEATTR 60
+ I+GAGAIGS L +AG +VLLI R + A+ G+ + G + V+V +
Sbjct: 3 RFVIIGAGAIGSFLSTRLHDAGHEVLLIARGARLEALRNMGVLLSEGGAERQVRVPVSAA 62
Query: 61 V-PEEKPDLIVLATKSYSTIEALKSARHIVKGS--WVLSIQNGIGNEDKIIEFGGKAIG- 116
P PD +LATK++ IE+ + ++GS +L++QNG+ +A+
Sbjct: 63 CDPATPPDHAILATKTFQ-IESALALLEPLRGSRFTLLTVQNGVEAPGIAQAALPRAVVL 121
Query: 117 GITTNGAMVEAPGVIKWTGKGVTII-------GLYPQGKEKFIEKVADVFNSADIETHVS 169
G +G G+++ TG T++ P + D+ +A + T ++
Sbjct: 122 GARMHGFFELEDGIVRHTGVPATLLAGPVADHATDPAAGQASANLAGDLI-AAGVPTTLT 180
Query: 170 ENIISWIWAKAIVNSAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIEFD 229
++ +W K ++ + I + +K + + ++ E + E R+A GIE
Sbjct: 181 GDMRPALWDKFLMAATIGAVAPAFGLKVGQVLSHPQASALLREAMAEVERIATALGIELP 240
Query: 230 VPPMDLFFQTLEQTRENYNSMLQ-DIWRGKKTEVDYINGKIVEYAKAVNLEAP 281
+D + + + S LQ ++ G+ +E +++G I +A+ +L P
Sbjct: 241 PDCIDEKLDFIGRFPPDVTSSLQRNLEAGRPSEFVHLSGAIPRFARQADLPCP 293
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.316 0.137 0.393
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 109454491
Number of Sequences: 2977
Number of extensions: 4528629
Number of successful extensions: 14942
Number of sequences better than 1.0e-10: 18
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 14903
Number of HSP's gapped (non-prelim): 19
length of query: 300
length of database: 189,106,746
effective HSP length: 56
effective length of query: 244
effective length of database: 155,591,474
effective search space: 37964319656
effective search space used: 37964319656
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 165 (68.7 bits)