BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB0536 (thi1) DE:thiamine biosynthetic enzyme (252 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||H75123 thiamin biosynthetic enzyme PAB0536 - Pyrococcus aby... 499 e-140 pir||G71007 thiamin biosynthesis protein thi1 homolog - Pyrococc... 473 e-133 sp|O27657|THI4_METTH PUTATIVE THIAZOLE BIOSYNTHETIC ENZYME >gi|7... 310 7e-84 pir||D72333 thiamin biosynthesis protein thi1 homolog - Thermoto... 266 2e-70 sp|Q58018|THI4_METJA PUTATIVE THIAZOLE BIOSYNTHETIC ENZYME >gi|2... 248 4e-65 gb|AAK40761.1| Thiazole biosynthetic enzyme [Sulfolobus solfatar... 233 2e-60 gi|11498309 thiamine biosynthetic enzyme (thi1) [Archaeoglobus f... 223 1e-57 pir||H72521 probable thiazole biosynthetic enzyme APE2149 - Aero... 216 2e-55 dbj|BAA88228.1| (AB030493) thiamin biosynthetic enzyme [Glycine ... 187 1e-46 dbj|BAA88226.1| (AB030491) thiamin biosynthetic enzyme [Glycine ... 186 2e-46 sp|Q38814|THI4_ARATH THIAZOLE BIOSYNTHETIC ENZYME PRECURSOR (ARA... 185 6e-46 dbj|BAA88227.1| (AB030492) thiamin biosynthetic enzyme [Glycine ... 184 1e-45 dbj|BAA88225.1| (AB030490) thiamin biosynthetic enzyme [Glycine ... 183 1e-45 sp|O23787|THI4_CITSI THIAZOLE BIOSYNTHETIC ENZYME PRECURSOR >gi|... 182 5e-45 sp|Q38709|THI4_ALNGL THIAZOLE BIOSYNTHETIC ENZYME PRECURSOR (AG6... 179 3e-44 sp|Q41739|TH42_MAIZE THIAMINE BIOSYNTHETIC ENZYME 1-2 PRECURSOR ... 177 1e-43 sp|Q41738|TH41_MAIZE THIAMINE BIOSYNTHETIC ENZYME 1-1 PRECURSOR ... 176 2e-43 pir||T40013 thiazole biosynthetic enzyme - fission yeast (Schiz... 172 3e-42 sp|P23617|THI4_FUSSH THIAZOLE BIOSYNTHETIC ENZYME PRECURSOR (STR... 166 2e-40 sp|P23618|THI4_FUSOX THIAZOLE BIOSYNTHETIC ENZYME PRECURSOR (STR... 163 2e-39 pir||JC7337 pyrithiamine resistance protein - Aspergillus oryzae... 161 8e-39 emb|CAC03570.1| (AJ297565) CyPBP37 protein [Neurospora crassa] 158 7e-38 sp|P40998|THI2_SCHPO THIAZOLE BIOSYNTHETIC ENZYME PRECURSOR >gi|... 151 9e-36 emb|CAB59856.1| (AJ250427) THI2p [Uromyces fabae] 148 4e-35 gb|AAG20644.1| (AE005133) thiamine biosynthetic enzyme; Thi1 [Ha... 142 3e-33 gi|6321583 component of the biosynthetic pathway producing the t... 122 3e-27 emb|CAB64776.1| (AJ250474) thiazole biosynthetic enzyme [Brassic... 75 9e-13 >pir||H75123 thiamin biosynthetic enzyme PAB0536 - Pyrococcus abyssi (strain Orsay) >gi|5458216|emb|CAB49705.1| (AJ248285) thiamine biosynthetic enzyme [Pyrococcus abyssi] Length = 252 Score = 499 bits (1271), Expect = e-140 Identities = 252/252 (100%), Positives = 252/252 (100%) Query: 1 MLREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKGGAKVAIFEKKLSIG 60 MLREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKGGAKVAIFEKKLSIG Sbjct: 1 MLREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKGGAKVAIFEKKLSIG 60 Query: 61 GGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTVKAGVKI 120 GGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTVKAGVKI Sbjct: 61 GGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTVKAGVKI 120 Query: 121 FNMIEVEDLVVKDNRVSGIVINWTPVLMTGLHVDPLTVEAKYVIDSTGHGAQVAQFLLKR 180 FNMIEVEDLVVKDNRVSGIVINWTPVLMTGLHVDPLTVEAKYVIDSTGHGAQVAQFLLKR Sbjct: 121 FNMIEVEDLVVKDNRVSGIVINWTPVLMTGLHVDPLTVEAKYVIDSTGHGAQVAQFLLKR 180 Query: 181 GLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIFGGMFLSGK 240 GLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIFGGMFLSGK Sbjct: 181 GLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIFGGMFLSGK 240 Query: 241 KAAQEILEKLNL 252 KAAQEILEKLNL Sbjct: 241 KAAQEILEKLNL 252 >pir||G71007 thiamin biosynthesis protein thi1 homolog - Pyrococcus horikoshii >gi|3257780|dbj|BAA30463.1| (AP000006) 255aa long hypothetical thiamine biosynthetic enzyme [Pyrococcus horikoshii] Length = 255 Score = 473 bits (1204), Expect = e-133 Identities = 233/250 (93%), Positives = 246/250 (98%) Query: 1 MLREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKGGAKVAIFEKKLSIG 60 MLREVTI+RAI+ESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKGGAKVAIFEKKLSIG Sbjct: 4 MLREVTITRAIVESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKGGAKVAIFEKKLSIG 63 Query: 61 GGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTVKAGVKI 120 GGIWGGGMGFNKVVVQ+EAREILDEF IRYEEFEKGYYVADAIEVATTIASK VK+GVKI Sbjct: 64 GGIWGGGMGFNKVVVQDEAREILDEFGIRYEEFEKGYYVADAIEVATTIASKVVKSGVKI 123 Query: 121 FNMIEVEDLVVKDNRVSGIVINWTPVLMTGLHVDPLTVEAKYVIDSTGHGAQVAQFLLKR 180 FNMIEVEDLV+KDNRVSGIVINWTPVLM GLHVDPLT+EAKYVIDSTGHGAQVAQFL+KR Sbjct: 124 FNMIEVEDLVIKDNRVSGIVINWTPVLMAGLHVDPLTIEAKYVIDSTGHGAQVAQFLVKR 183 Query: 181 GLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIFGGMFLSGK 240 GL++ IPGEGAMWAEQGE+LTV+NTREVFPGLYVTGMAANAIAGAPRMGPIFGGMFLSG+ Sbjct: 184 GLLKEIPGEGAMWAEQGEKLTVKNTREVFPGLYVTGMAANAIAGAPRMGPIFGGMFLSGR 243 Query: 241 KAAQEILEKL 250 KAAQEIL+KL Sbjct: 244 KAAQEILKKL 253 >sp|O27657|THI4_METTH PUTATIVE THIAZOLE BIOSYNTHETIC ENZYME >gi|7446891|pir||E69083 thiamin biosynthesis protein thi1 homolog - Methanobacterium thermoautotrophicum (strain Delta H) >gi|2622746|gb|AAB86093.1| (AE000921) thiamine biosynthetic enzyme [Methanobacterium thermoautotrophicum] Length = 266 Score = 310 bits (787), Expect = 7e-84 Identities = 144/255 (56%), Positives = 199/255 (77%), Gaps = 6/255 (2%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKGGAKVAIFEKKLSIGG 61 L ++ ISRAI+E Y DLL+ +E+DVAI G GPSG+ A YYLA+ G KVA+FE+KLSIGG Sbjct: 11 LDDIKISRAIVEGYMEDLLDYMEMDVAIGGGGPSGLTAGYYLARAGLKVALFERKLSIGG 70 Query: 62 GIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTVKAGVKIF 121 G+WGGGM FNK+VVQ+E REILDEF IR E +++GY+VAD++E +T+ S+ +AG+KIF Sbjct: 71 GMWGGGMMFNKIVVQDEGREILDEFGIRSEPYDEGYHVADSVEATSTLCSRACQAGLKIF 130 Query: 122 NMIEVEDLVVKDNRVSGIVINWTPVLMTGLHVDPLTVEAKYVIDSTGHGAQVAQFLLKRG 181 N++ +ED++++D ++G+V+NW+ V M GLHVDPLTV A+ VID+TGH ++ + + ++ Sbjct: 131 NLMSIEDVMIRDEGITGLVLNWSSVEMAGLHVDPLTVRARAVIDATGHDCEIVKVVERKI 190 Query: 182 LIE------RIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIFGGM 235 E RI GE +MWA+ GE +ENTREV+P LYV GMA+NA+ GAPRMGPIFGGM Sbjct: 191 GPELNTPDGRIQGERSMWADVGEAALIENTREVYPNLYVAGMASNAVYGAPRMGPIFGGM 250 Query: 236 FLSGKKAAQEILEKL 250 +SG++ A+ I+EKL Sbjct: 251 LVSGRRVAEMIIEKL 265 >pir||D72333 thiamin biosynthesis protein thi1 homolog - Thermotoga maritima (strain MSB8) >gi|4981316|gb|AAD35869.1|AE001747_12 (AE001747) thiamine biosynthetic enzyme [Thermotoga maritima] Length = 250 Score = 266 bits (672), Expect = 2e-70 Identities = 129/249 (51%), Positives = 179/249 (71%), Gaps = 2/249 (0%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKGGAKVAIFEKKLSIGG 61 +R+V ISR I+E Y+ L N+LELDVAIVGAGPSG+ AAY LAK G +VA+FE++ + GG Sbjct: 3 MRDVLISRLIVERYFEKLRNSLELDVAIVGAGPSGLTAAYELAKNGFRVAVFEERNTPGG 62 Query: 62 GIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTVKAGVKIF 121 GIWGGGM FN++V+++E L E +I Y E ++ + V D++ A+ + + KAG +F Sbjct: 63 GIWGGGMMFNEIVLEKELENFLKEVEIEY-EVKEDHIVVDSVHFASGLLYRATKAGAIVF 121 Query: 122 NMIEVEDLVVKDNRVSGIVINWTPVLMTGLHVDPLTVEAKYVIDSTGHGAQVAQFLLKRG 181 N + VED+ V++ RV G+V+NW P + GLHVDP+TV+A +V+D TGH A V L KRG Sbjct: 122 NNVSVEDVAVQNGRVCGVVVNWGPTVRLGLHVDPITVKASFVVDGTGHPANVVSLLAKRG 181 Query: 182 LIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIFGGMFLSGKK 241 L+E + E M A++ E+ V+NT E+FPGL V+GMA A+ G PRMGPIFGGM LSG+K Sbjct: 182 LVE-MKTEFPMDADEAEKFVVDNTGEIFPGLLVSGMAVCAVHGGPRMGPIFGGMILSGQK 240 Query: 242 AAQEILEKL 250 A+ + E+L Sbjct: 241 VARIVSERL 249 >sp|Q58018|THI4_METJA PUTATIVE THIAZOLE BIOSYNTHETIC ENZYME >gi|2129302|pir||A64375 thiamin biosynthesis protein thi1 homolog - Methanococcus jannaschii >gi|1591310|gb|AAB98592.1| (U67508) thiamine biosynthetic enzyme (thi1) [Methanococcus jannaschii] Length = 267 Score = 248 bits (627), Expect = 4e-65 Identities = 124/248 (50%), Positives = 170/248 (68%), Gaps = 1/248 (0%) Query: 4 EVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKGGAKVAIFEKKLSIGGGI 63 E ++AI+++ + L+ +E DV IVGAGPSG+ A YLAK G KV + E+ L+ GGG Sbjct: 16 ETKTTKAILKASFDMWLDIVEADVVIVGAGPSGLTCARYLAKEGFKVVVLERHLAFGGGT 75 Query: 64 WGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTVKAGVKIFNM 123 WGGGMGF +VV+E A E+L E I+ + GYYVAD++EV +A + AG KI Sbjct: 76 WGGGMGFPYIVVEEPADELLREVGIKLIDMGDGYYVADSVEVPAKLAVAAMDAGAKILTG 135 Query: 124 IEVEDLVVKDNRVSGIVINWTPVLMTGLHVDPLTVEAKYVIDSTGHGAQVAQFLLKRGLI 183 I VEDL+++++ V+G+VIN + GLH+DPLT+ +K V+D+TGH A + L+K+ + Sbjct: 136 IVVEDLILREDGVAGVVINSYAIERAGLHIDPLTIRSKVVVDATGHEASIVNILVKKNKL 195 Query: 184 ER-IPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIFGGMFLSGKKA 242 E +PGE +MWAE+GE + NTREV+P L+V GMAANA G RMG IFGGM+LSGK Sbjct: 196 EADVPGEKSMWAEKGENALLRNTREVYPNLFVCGMAANASHGGYRMGAIFGGMYLSGKLC 255 Query: 243 AQEILEKL 250 A+ I EKL Sbjct: 256 AELITEKL 263 >gb|AAK40761.1| Thiazole biosynthetic enzyme [Sulfolobus solfataricus] Length = 267 Score = 233 bits (588), Expect = 2e-60 Identities = 122/253 (48%), Positives = 168/253 (66%), Gaps = 8/253 (3%) Query: 4 EVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKGGAKVAIFEKKLSIGGGI 63 EV ISR II+ D +E DV IVGAGPSG+ AAYYLAK G K +FE++LS GGGI Sbjct: 10 EVKISRYIIKETMEDWYQFVESDVVIVGAGPSGLSAAYYLAKAGLKTLVFERRLSFGGGI 69 Query: 64 WGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTVKAGVKIFNM 123 GG M F+K+++++ A EIL E +IR +E E+G YV D+ E +A+ + AG KI + Sbjct: 70 GGGAMLFHKLIIEKPADEILREVNIRLKEVEEGVYVVDSAEFMAKLATAAIDAGAKIIHG 129 Query: 124 IEVEDLVVKDN--RVSGIVINWTPVLMTGLHVDPLTVEAKYVIDSTGHGAQV----AQFL 177 + V+D++ ++N RV+G+ + WT M LHVDP+ + AK V+D+TGH A+V A+ + Sbjct: 130 VTVDDVIFRENPLRVAGVAVEWTATQMASLHVDPIFISAKAVVDATGHDAEVISVAARKI 189 Query: 178 LKRGLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIFGGMFL 237 + G++ IPGE + ++E+ E LTV NT +V GLY TGMA + G PRMGPIFG M L Sbjct: 190 PELGIV--IPGEKSAYSERAEELTVINTGKVAEGLYATGMAVTEVKGLPRMGPIFGAMVL 247 Query: 238 SGKKAAQEILEKL 250 SGK A EI + L Sbjct: 248 SGKAVAGEITKDL 260 >gi|11498309 thiamine biosynthetic enzyme (thi1) [Archaeoglobus fulgidus] >gi|6094475|sp|O29556|THI4_ARCFU PUTATIVE THIAZOLE BIOSYNTHETIC ENZYME >gi|7446890|pir||F69337 thiamin biosynthesis protein thi1 homolog - Archaeoglobus fulgidus >gi|2649913|gb|AAB90538.1| (AE001056) thiamine biosynthetic enzyme (thi1) [Archaeoglobus fulgidus] Length = 260 Score = 223 bits (563), Expect = 1e-57 Identities = 116/252 (46%), Positives = 167/252 (66%), Gaps = 4/252 (1%) Query: 4 EVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKGGAKVAIFEKKLSIGGGI 63 E I++AI+E+ + + E DV +VGAGPSG+ AA YLA+ G K + E++LS GGGI Sbjct: 10 EAEITKAIVETASEEWVEYAESDVIVVGAGPSGLTAARYLAEKGLKTLVLERRLSFGGGI 69 Query: 64 WGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTVKAGVKIFNM 123 GGGM F+KVVV+ EA++ILD+F IRY E + + VAD+ E +A+K + AG KI + Sbjct: 70 GGGGMLFHKVVVEREAKDILDDFGIRYTE-HRNFLVADSAEFMAKLAAKAIDAGAKIIHG 128 Query: 124 IEVEDLVVKDN--RVSGIVINWTPVLMTGLHVDPLTVEAKYVIDSTGHGAQVAQFLLKRG 181 + VED++ +D+ V G+ I W+ V ++GLHVDPL + ++ V+D+TGH A+V ++ Sbjct: 129 VSVEDVIFRDDPLGVRGVCIQWSAVEISGLHVDPLFLRSRAVVDATGHDAEVISVAARKI 188 Query: 182 LIE-RIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIFGGMFLSGK 240 +E + GE + ++E ER VE T ++ GLY GMA A+ PRMGPIFGGM LSGK Sbjct: 189 PLEVSVVGERSAYSEVAEREIVEKTGKIVKGLYAAGMAVAAVHNLPRMGPIFGGMLLSGK 248 Query: 241 KAAQEILEKLNL 252 K A+ + E L L Sbjct: 249 KVAEIVAEDLKL 260 >pir||H72521 probable thiazole biosynthetic enzyme APE2149 - Aeropyrum pernix (strain K1) >gi|5105848|dbj|BAA81160.1| (AP000063) 274aa long hypothetical thiazole biosynthetic enzyme [Aeropyrum pernix] Length = 274 Score = 216 bits (544), Expect = 2e-55 Identities = 110/256 (42%), Positives = 166/256 (63%), Gaps = 8/256 (3%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKGGAKVAIFEKKLSIGG 61 + E ++ AI++ +Y L +L DV +VGAGP+G+ AA+ LA+ GA+V I E+ +GG Sbjct: 10 VNEADVTEAILDGFYSSLKKHLRSDVIVVGAGPAGLTAAWRLAEAGARVLIVEQNNYLGG 69 Query: 62 GIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTVKAGVKIF 121 G+W GG N V ++ A+ ILDE ++ YE + G Y +A +A++ ++AG ++ Sbjct: 70 GLWLGGYFMNPVTIRAPAQRILDELEVPYEAVKPGLYRTKGPLLAAKLAARALEAGAEVL 129 Query: 122 NMIEVEDLVVKDNRVSGIVINWTPVLMTGLH-----VDPLTVEAKYVIDSTGHGAQVAQF 176 N+ ++D++V+++RV+G+V+NW+PV GL VDP+ + A+YV+D+TGH A V + Sbjct: 130 NLTMLDDVIVENSRVAGVVVNWSPV--QGLPRQITCVDPVGLRAEYVVDATGHDAVVTRK 187 Query: 177 LLKRGLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIFGGMF 236 L +RG++E G MW E+ E L VE T EV+PGL V G+A + G PRMGP FG M Sbjct: 188 LAERGMVE-ASKLGPMWVERSEDLVVEKTGEVYPGLVVAGIAVAEVYGLPRMGPTFGAML 246 Query: 237 LSGKKAAQEILEKLNL 252 LSG+KAA I EKL L Sbjct: 247 LSGEKAAALIGEKLGL 262 >dbj|BAA88228.1| (AB030493) thiamin biosynthetic enzyme [Glycine max] Length = 349 Score = 187 bits (470), Expect = 1e-46 Identities = 109/260 (41%), Positives = 150/260 (56%), Gaps = 9/260 (3%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKG-GAKVAIFEKKLSIG 60 +RE +SR + Y D++ + + DV +VGAG +G+ AY L+K +AI E+ +S G Sbjct: 63 IRESIVSREMTRRYMTDMVTHADTDVVVVGAGSAGLSCAYELSKNPSVNIAIVEQSISPG 122 Query: 61 GGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTV-KAGVK 119 GG W GG F+ +VV++ A LDE + Y+E + + A +TI SK + + VK Sbjct: 123 GGAWLGGQLFSAMVVRKPAHLFLDELGLEYDEQDNYVVIKHAALFTSTIMSKLLARPNVK 182 Query: 120 IFNMIEVEDLVVKDNRVSGIVINWTPVLM---TGLHVDPLTVEAKYVIDSTGH----GAQ 172 +FN + EDL+VK+ RV G+V NW V M T +DP +EAK V+ S GH GA Sbjct: 183 LFNAVAAEDLIVKNGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGAT 242 Query: 173 VAQFLLKRGLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIF 232 + L GLI+ +PG A+ + E VE TREV PG+ VTGM I GAPRMGP F Sbjct: 243 GVKRLKSIGLIDSVPGMKALDMNKAEDAIVELTREVVPGMIVTGMEVAEIDGAPRMGPTF 302 Query: 233 GGMFLSGKKAAQEILEKLNL 252 G M +SG+KAA L L L Sbjct: 303 GAMMISGQKAAHLALRSLGL 322 >dbj|BAA88226.1| (AB030491) thiamin biosynthetic enzyme [Glycine max] Length = 349 Score = 186 bits (467), Expect = 2e-46 Identities = 108/260 (41%), Positives = 151/260 (57%), Gaps = 9/260 (3%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKG-GAKVAIFEKKLSIG 60 +RE +SR + Y D++ + + DV +VGAG +G+ AY L+K +AI E+ +S G Sbjct: 63 IRESIVSREMTRRYMTDMVTHADTDVVVVGAGSAGLSCAYELSKNPSVNIAIVEQSVSPG 122 Query: 61 GGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTV-KAGVK 119 GG W GG F+ +VV++ A LDE + Y+E + + A + +TI SK + + VK Sbjct: 123 GGAWLGGQLFSAMVVRKPAHLFLDELGLEYDEQDNYVVIKHAALLTSTIMSKLLARPNVK 182 Query: 120 IFNMIEVEDLVVKDNRVSGIVINWTPVLM---TGLHVDPLTVEAKYVIDSTGH----GAQ 172 +FN + EDL+VK+ RV G+V NW V M T +DP +EAK V+ S GH GA Sbjct: 183 LFNAVAAEDLIVKNGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGAT 242 Query: 173 VAQFLLKRGLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIF 232 + L GLI+ +PG A+ + E V+ TREV PG+ VTGM I GAPRMGP F Sbjct: 243 GVKRLKSIGLIDSVPGMKALDMNKAEDAIVKLTREVVPGMIVTGMEVAEIDGAPRMGPTF 302 Query: 233 GGMFLSGKKAAQEILEKLNL 252 G M +SG+KAA L L L Sbjct: 303 GAMMISGQKAAHLALRSLGL 322 >sp|Q38814|THI4_ARATH THIAZOLE BIOSYNTHETIC ENZYME PRECURSOR (ARA6) >gi|2129750|pir||S71191 thiamin biosynthesis protein thi4 - Arabidopsis thaliana >gi|1113783|gb|AAC97124.1| (U17589) Thi1 protein [Arabidopsis thaliana] >gi|9758257|dbj|BAB08756.1| (AB005232) thiazole biosynthetic enzyme precursor (ARA6) [Arabidopsis thaliana] Length = 349 Score = 185 bits (464), Expect = 6e-46 Identities = 108/260 (41%), Positives = 149/260 (56%), Gaps = 9/260 (3%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKG-GAKVAIFEKKLSIG 60 ++E +SR + Y D++ E DV +VGAG +G+ AAY ++K +VAI E+ +S G Sbjct: 61 IKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAIIEQSVSPG 120 Query: 61 GGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTV-KAGVK 119 GG W GG F+ ++V++ A LDE + Y+E + V A +TI SK + + VK Sbjct: 121 GGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKLLARPNVK 180 Query: 120 IFNMIEVEDLVVKDNRVSGIVINWTPVLM---TGLHVDPLTVEAKYVIDSTGH----GAQ 172 +FN + EDL+VK NRV G+V NW V T +DP +EAK V+ S GH GA Sbjct: 181 LFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGHDGPFGAT 240 Query: 173 VAQFLLKRGLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIF 232 + L G+I+ +PG A+ E V TREV PG+ VTGM I GAPRMGP F Sbjct: 241 GVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTF 300 Query: 233 GGMFLSGKKAAQEILEKLNL 252 G M +SG+KA Q L+ L L Sbjct: 301 GAMMISGQKAGQLALKALGL 320 >dbj|BAA88227.1| (AB030492) thiamin biosynthetic enzyme [Glycine max] Length = 345 Score = 184 bits (462), Expect = 1e-45 Identities = 108/260 (41%), Positives = 151/260 (57%), Gaps = 9/260 (3%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKGGA-KVAIFEKKLSIG 60 +RE +SR + Y D++ + + DV IVGAG +G+ AY L+K + +AI E+ +S G Sbjct: 59 IRESIVSREMTRRYMIDMVTHADTDVVIVGAGSAGLSCAYELSKNPSINIAIVEQSVSPG 118 Query: 61 GGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTV-KAGVK 119 GG W GG F+ +VV++ A LDE ++ Y+E + + A +TI SK + + VK Sbjct: 119 GGAWLGGQLFSAMVVRKPAHLFLDELNVEYDEQDNYVVIKHAALFTSTIMSKLLARPNVK 178 Query: 120 IFNMIEVEDLVVKDNRVSGIVINWTPVLM---TGLHVDPLTVEAKYVIDSTGH----GAQ 172 +FN + EDL+VK+ RV G+V NW V + T +DP +EAK V+ S GH GA Sbjct: 179 LFNAVAAEDLIVKNGRVGGVVTNWALVSLNHDTQSCMDPNVMEAKVVVSSCGHDGPFGAT 238 Query: 173 VAQFLLKRGLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIF 232 + L GLI+ +PG A+ + E V TREV PG+ VTGM I GAPRMGP F Sbjct: 239 GVKRLKSIGLIDSVPGMKALDMNKAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTF 298 Query: 233 GGMFLSGKKAAQEILEKLNL 252 G M +SG+KAA L L L Sbjct: 299 GAMMISGQKAAHLALRSLGL 318 >dbj|BAA88225.1| (AB030490) thiamin biosynthetic enzyme [Glycine max] Length = 345 Score = 183 bits (461), Expect = 1e-45 Identities = 108/260 (41%), Positives = 151/260 (57%), Gaps = 9/260 (3%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKGGA-KVAIFEKKLSIG 60 +RE +SR + Y D++ + + DV IVGAG +G+ AY L+K + +AI E+ +S G Sbjct: 59 IRESIVSREMTRRYMIDMVTHADTDVVIVGAGSAGLSCAYELSKNPSINIAIVEQSVSPG 118 Query: 61 GGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTV-KAGVK 119 GG W GG F+ +VV++ A LDE ++ Y+E + + A +TI SK + + VK Sbjct: 119 GGAWLGGQLFSAMVVRKPAHLFLDELNLEYDEQDNYVVIKHAALFTSTIMSKLLARPNVK 178 Query: 120 IFNMIEVEDLVVKDNRVSGIVINWTPVLM---TGLHVDPLTVEAKYVIDSTGH----GAQ 172 +FN + EDL+VK+ RV G+V NW V + T +DP +EAK V+ S GH GA Sbjct: 179 LFNAVAAEDLIVKNGRVGGVVTNWALVSLNHDTQSCMDPNVMEAKVVVSSCGHDGPFGAT 238 Query: 173 VAQFLLKRGLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIF 232 + L GLI+ +PG A+ + E V TREV PG+ VTGM I GAPRMGP F Sbjct: 239 GVKRLKSIGLIDSVPGMKALDMNKAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTF 298 Query: 233 GGMFLSGKKAAQEILEKLNL 252 G M +SG+KAA L L L Sbjct: 299 GAMMISGQKAAHLALRSLGL 318 >sp|O23787|THI4_CITSI THIAZOLE BIOSYNTHETIC ENZYME PRECURSOR >gi|7446894|pir||T10474 thiamin biosynthesis protein thi1 - sweet orange >gi|2582665|emb|CAB05370.1| (Z82983) thi [Citrus sinensis] Length = 356 Score = 182 bits (456), Expect = 5e-45 Identities = 107/258 (41%), Positives = 147/258 (56%), Gaps = 9/258 (3%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKG-GAKVAIFEKKLSIG 60 ++E +SR + Y D++ + DV +VGAG +G+ AY L+K ++AI E+ +S G Sbjct: 68 IKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQIAIIEQSVSPG 127 Query: 61 GGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTV-KAGVK 119 GG W GG F+ +VV++ A LDE I Y+E + + A +TI SK + + VK Sbjct: 128 GGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIMSKLLARPNVK 187 Query: 120 IFNMIEVEDLVVKDNRVSGIVINWTPVLM---TGLHVDPLTVEAKYVIDSTGH----GAQ 172 +FN + EDL+VK RV G+V NW V M T +DP +EAK V+ S GH GA Sbjct: 188 LFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGAT 247 Query: 173 VAQFLLKRGLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIF 232 + L G+IE +PG A+ E V TREV PG+ VTGM I GAPRMGP F Sbjct: 248 GVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTF 307 Query: 233 GGMFLSGKKAAQEILEKL 250 G M +SG+KAA L+ L Sbjct: 308 GAMMISGQKAAHLALKSL 325 >sp|Q38709|THI4_ALNGL THIAZOLE BIOSYNTHETIC ENZYME PRECURSOR (AG6) >gi|1289204|emb|CAA66064.1| (X97434) thaizole biosynthetic enzmye [Alnus glutinosa] Length = 352 Score = 179 bits (449), Expect = 3e-44 Identities = 108/260 (41%), Positives = 148/260 (56%), Gaps = 10/260 (3%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKG-GAKVAIFEKKLSIG 60 ++E +SR + Y D++ + DV +VGAG SG+V Y L+K +VAI E+ +S G Sbjct: 65 IKESIVSREMTRRYMMDMITYADTDVVVVGAGSSGLV-CYELSKNPSVQVAIIEQSVSPG 123 Query: 61 GGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTV-KAGVK 119 GG W GG F+ +VV++ A LDE I Y+E + + A +TI SK + + VK Sbjct: 124 GGAWLGGQLFSGMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTIMSKLLARPNVK 183 Query: 120 IFNMIEVEDLVVKDNRVSGIVINWTPVLM---TGLHVDPLTVEAKYVIDSTGH----GAQ 172 +FN + EDL+VK RV G+V NW V M T +DP +EAK V+ S GH GA Sbjct: 184 LFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGAT 243 Query: 173 VAQFLLKRGLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIF 232 + L G+I+ +PG A+ E V TRE+ PG+ VTGM I GAPRMGP F Sbjct: 244 GVKSLRSIGMIDTVPGMKALDMNVAEDAIVRLTREIVPGMIVTGMEVAEIDGAPRMGPTF 303 Query: 233 GGMFLSGKKAAQEILEKLNL 252 G M +SG+KAA L+ L L Sbjct: 304 GAMMISGQKAAHLALKALGL 323 >sp|Q41739|TH42_MAIZE THIAMINE BIOSYNTHETIC ENZYME 1-2 PRECURSOR >gi|2130147|pir||S61420 thiamin biosynthesis protein thi1-2 - maize >gi|596080|gb|AAA96739.1| (U17351) thiamine biosynthetic enzyme [Zea mays] Length = 354 Score = 177 bits (444), Expect = 1e-43 Identities = 107/258 (41%), Positives = 146/258 (56%), Gaps = 9/258 (3%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKGGA-KVAIFEKKLSIG 60 ++E +SR + Y D++ + + DV IVGAG +G+ AY L+K VAI E+ +S G Sbjct: 64 IKESIVSREMTRRYMTDMITHADTDVVIVGAGSAGLSCAYELSKDPTVSVAIVEQSVSPG 123 Query: 61 GGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTV-KAGVK 119 GG W GG F+ +VV+ A LDE + Y+E E V A +T+ S+ + + VK Sbjct: 124 GGAWLGGQLFSAMVVRRPAHLFLDELGVGYDEAEDYVVVKHAALFTSTVMSRLLARPNVK 183 Query: 120 IFNMIEVEDLVVKDNRVSGIVINWTPVLM---TGLHVDPLTVEAKYVIDSTGH----GAQ 172 +FN + VEDL+V+ RV G+V NW V M T +DP +EAK V+ S GH GA Sbjct: 184 LFNAVAVEDLIVRRGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGAT 243 Query: 173 VAQFLLKRGLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIF 232 + L G+I +PG A+ E V TREV PG+ VTGM I GAPRMGP F Sbjct: 244 GVKRLQDIGMISAVPGMKALDMNAAEDEIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTF 303 Query: 233 GGMFLSGKKAAQEILEKL 250 G M +SG+KAA L+ L Sbjct: 304 GAMMISGQKAAHLALKAL 321 >sp|Q41738|TH41_MAIZE THIAMINE BIOSYNTHETIC ENZYME 1-1 PRECURSOR >gi|2130146|pir||S61419 thiamin biosynthesis protein thi1-1 - maize >gi|596078|gb|AAA96738.1| (U17350) thiamine biosynthetic enzyme [Zea mays] Length = 354 Score = 176 bits (442), Expect = 2e-43 Identities = 106/258 (41%), Positives = 146/258 (56%), Gaps = 9/258 (3%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKGGA-KVAIFEKKLSIG 60 ++E +SR + Y D++ + DV IVGAG +G+ AY L+K A +AI E+ +S G Sbjct: 67 IKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKDPAVSIAIVEQSVSPG 126 Query: 61 GGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTV-KAGVK 119 GG W GG F+ +VV++ A LDE + Y+E E + A +T+ S + + VK Sbjct: 127 GGAWLGGQLFSAMVVRKPAHLFLDELGVAYDEAEDYVVIKHAALFTSTVMSLLLARPNVK 186 Query: 120 IFNMIEVEDLVVKDNRVSGIVINWTPVLM---TGLHVDPLTVEAKYVIDSTGH----GAQ 172 +FN + VEDL+V+ RV G+V NW V M T +DP +EAK V+ S GH GA Sbjct: 187 LFNAVAVEDLIVRGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGAT 246 Query: 173 VAQFLLKRGLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIF 232 + L G+I +PG A+ E V TREV PG+ VTGM I GAPRMGP F Sbjct: 247 GVKRLQDIGMISAVPGMKALDMNTAEDEIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTF 306 Query: 233 GGMFLSGKKAAQEILEKL 250 G M +SG+KAA L+ L Sbjct: 307 GAMMISGQKAAHLALKAL 324 >pir||T40013 thiazole biosynthetic enzyme - fission yeast (Schizosaccharomyces pombe) >gi|3650384|emb|CAA21093.1| (AL031743) thiazole biosynthetic enzyme. [Schizosaccharomyces pombe] Length = 328 Score = 172 bits (432), Expect = 3e-42 Identities = 107/263 (40%), Positives = 149/263 (55%), Gaps = 19/263 (7%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLA--KGGAKVAIFEKKLSI 59 +RE T+SRA+ Y+ DL E D+ IVGAG +G+ AAYY+ + K+AI E ++ Sbjct: 54 IRESTVSRAMTRRYFSDLDKYAESDIVIVGAGSAGLTAAYYIGTRRPDLKIAIIEASVAP 113 Query: 60 GGGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVA---DAIEVATTIASKTVKA 116 GGG W GG F+ +VV++ A L+E + YE+ +G YV A+ +T +A Sbjct: 114 GGGAWLGGQLFSAMVVRKPADLFLNEIGVPYED--EGDYVVVKHAALFTSTVMARTLALP 171 Query: 117 GVKIFNMIEVEDLVVKDN-----RVSGIVINWTPVLMT-GLH--VDPLTVEAKYVIDSTG 168 VK+FN VEDL+VK+ R++G+V NWT V + GL +DP T+ A V+ +TG Sbjct: 172 NVKLFNATAVEDLIVKEGKDGKQRIAGVVTNWTLVSLNHGLQSCMDPNTINAHLVVSATG 231 Query: 169 HGAQVAQFLLKR----GLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAG 224 H F +KR L+ + + + E L V+ TREVFPG+ V GM + G Sbjct: 232 HDGPFGAFCVKRLASAQLVSNLHDMRPLDMNRAEDLIVKGTREVFPGMIVGGMELSEFDG 291 Query: 225 APRMGPIFGGMFLSGKKAAQEIL 247 A RMGP FGGM SG KAAQE L Sbjct: 292 ANRMGPTFGGMMFSGIKAAQEAL 314 >sp|P23617|THI4_FUSSH THIAZOLE BIOSYNTHETIC ENZYME PRECURSOR (STRESS-INDUCIBLE PROTEIN STI35) >gi|102018|pir||A37767 stress-inducible protein sti35 - fungus (Fusarium solani) >gi|168162|gb|AAA33340.1| (M33642) STI35 protein [Fusarium solani] Length = 324 Score = 166 bits (416), Expect = 2e-40 Identities = 105/265 (39%), Positives = 147/265 (54%), Gaps = 14/265 (5%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKG--GAKVAIFEKKLSI 59 +RE +SRA+ Y++DL N E D+ IVGAG G+ Y L K K+AI E +S Sbjct: 53 IRESQVSRAMTRRYFQDLDNYAESDIVIVGAGSCGLSTRYILGKKRPDLKIAIIEASVSP 112 Query: 60 GGGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTVKA-GV 118 GGG W GG F+ +V+++ A L E + YE+ V A +TI SK ++ Sbjct: 113 GGGAWLGGQLFSAMVMRKPADAFLREVGVPYEDEGNYVVVKHAALFTSTIMSKVLQLPNC 172 Query: 119 KIFNMIEVEDLVVKDN----RVSGIVINWTPVLM---TGLHVDPLTVEAKYVIDSTGHGA 171 K+FN VEDL+ + + R+SG+V NWT V M +DP T+ A VI +TGH A Sbjct: 173 KLFNATCVEDLITRPSKEGVRISGVVTNWTLVSMHHDDQSCMDPNTINAPLVISTTGHDA 232 Query: 172 QVAQFLLKR----GLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPR 227 + F +KR G IE++ G + E V+ TRE+ PGL V GM + + GA R Sbjct: 233 PMGAFCVKRLVSMGRIEKLGGMRGLDMNVAEDAIVKGTREIVPGLIVGGMELSEVDGANR 292 Query: 228 MGPIFGGMFLSGKKAAQEILEKLNL 252 MGP FG M LSG KAA+E L+ +++ Sbjct: 293 MGPTFGAMVLSGLKAAEEALKVIDI 317 >sp|P23618|THI4_FUSOX THIAZOLE BIOSYNTHETIC ENZYME PRECURSOR (STRESS-INDUCIBLE PROTEIN STI35) >gi|280494|pir||B37767 stress-inducible protein sti35 - fungus (Fusarium oxysporum) >gi|168164|gb|AAA33341.1| (M33643) STI35 protein [Fusarium oxysporum] >gi|6045153|dbj|BAA85305.1| (AB033416) stress-responsive gene product [Fusarium oxysporum] Length = 320 Score = 163 bits (408), Expect = 2e-39 Identities = 101/261 (38%), Positives = 146/261 (55%), Gaps = 14/261 (5%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKG--GAKVAIFEKKLSI 59 +RE +SRA+ Y++DL N E D+ I+GAG G+ AAY L K K+AI E +S Sbjct: 49 IRESQVSRAMTRRYFQDLDNYAESDIVIIGAGSCGLSAAYILGKKRPDLKIAIIEASVSP 108 Query: 60 GGGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTVKA-GV 118 GGG W GG F+ +++++ A L E + YE+ V A +TI SK ++ + Sbjct: 109 GGGAWLGGQLFSAMIMRKPADAFLREVGVPYEDEGNYVVVKHAALFTSTIMSKVLQMPNI 168 Query: 119 KIFNMIEVEDLVVKDN----RVSGIVINWTPVLM---TGLHVDPLTVEAKYVIDSTGHGA 171 K+FN VEDL+ + + R++G+V NWT V M +DP T+ A +I +TGH Sbjct: 169 KLFNATCVEDLITRPSEEGVRIAGVVTNWTLVSMHHDDQSCMDPNTINAPLIISTTGHDG 228 Query: 172 QVAQFLLKRGL----IERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPR 227 + F +KR + IE++ G + E V+ TRE+ PGL V GM + + GA R Sbjct: 229 PMGAFCVKRLVSMQRIEKLGGMRGLDMNLAEDAIVKGTREIVPGLIVGGMELSEVDGANR 288 Query: 228 MGPIFGGMFLSGKKAAQEILE 248 MGP FG M LSG KAA+E L+ Sbjct: 289 MGPTFGAMALSGLKAAEEALK 309 >pir||JC7337 pyrithiamine resistance protein - Aspergillus oryzae >gi|6694867|gb|AAF25444.1|AF217503_1 (AF217503) putative thiazole synthase [Aspergillus oryzae] Length = 327 Score = 161 bits (403), Expect = 8e-39 Identities = 105/266 (39%), Positives = 146/266 (54%), Gaps = 21/266 (7%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKG--GAKVAIFEKKLSI 59 +RE +SRA+ Y+ DL E DV IVGAG G+ AY LAK K+AI E +S Sbjct: 53 IRESQVSRAMTRRYFEDLDKYAESDVVIVGAGSCGLSTAYVLAKARPDLKIAIVEASVSP 112 Query: 60 GGGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVA--DAIEVATTIASKTVK-A 116 GGG W GG F+ +V++ A L+E + YEE YV A +T+ SK + Sbjct: 113 GGGAWLGGQLFSAMVMRRPAEVFLNELGVPYEEDANPNYVVVKHASLFTSTLMSKVLSFP 172 Query: 117 GVKIFNMIEVEDLVVKDN-----RVSGIVINWTPVLMTGLH-----VDPLTVEAKYVIDS 166 VK+FN VEDL+ + +++G+V+NWT L+T H +DP T+ A +I + Sbjct: 173 NVKLFNATAVEDLITRPTENGNPQIAGVVVNWT--LVTLHHDDHSCMDPNTINAPVIIST 230 Query: 167 TGH----GAQVAQFLLKRGLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAI 222 TGH GA A+ L+ G ++++ G + E V+NTREV GL + GM + I Sbjct: 231 TGHDGPFGAFCAKRLVSMGSVDKLGGMRGLDMNSAEDAIVKNTREVTKGLIIGGMELSEI 290 Query: 223 AGAPRMGPIFGGMFLSGKKAAQEILE 248 G RMGP FG M LSG KAA+E L+ Sbjct: 291 DGFNRMGPTFGAMVLSGVKAAEEALK 316 >emb|CAC03570.1| (AJ297565) CyPBP37 protein [Neurospora crassa] Length = 344 Score = 158 bits (395), Expect = 7e-38 Identities = 102/280 (36%), Positives = 147/280 (52%), Gaps = 29/280 (10%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLA--KGGAKVAIFEKKLSI 59 +RE T+SRA+ Y+ DL + E D+ IVGAG G+ AAY L+ + ++ I E ++ Sbjct: 57 IRESTVSRAMTRRYFADLDAHAETDIVIVGAGSCGLSAAYVLSTLRPDLRITIVEAGVAP 116 Query: 60 GGGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTV-KAGV 118 GGG W GG F+ +V+++ A LDE + YE+ V A +T+ SK + + V Sbjct: 117 GGGAWLGGQLFSAMVMRKPADVFLDEVGVPYEDEGDYVVVKHAALFTSTVLSKVLQRPNV 176 Query: 119 KIFNMIEVEDLVVKDN-------------------RVSGIVINWTPVLM---TGLHVDPL 156 K+FN VEDL+ + + R++G+V NWT V M +DP Sbjct: 177 KLFNATTVEDLITRKHHAESSSSSDDGEAEDEAKVRIAGVVTNWTLVSMHHDDQSCMDPN 236 Query: 157 TVEAKYVIDSTGHGAQVAQFLLKRGL----IERIPGEGAMWAEQGERLTVENTREVFPGL 212 T+ A +I +TGH F +KR + +ER+ G + + E V NTRE+ PGL Sbjct: 237 TINAPVIISTTGHDGPFGAFSVKRLVSMKQMERLNGMRGLDMQSAEDAIVNNTREIVPGL 296 Query: 213 YVTGMAANAIAGAPRMGPIFGGMFLSGKKAAQEILEKLNL 252 V GM + I GA RMGP FG M LSG KAA E + +L Sbjct: 297 IVGGMELSEIDGANRMGPTFGAMALSGVKAAHEAIRVFDL 336 >sp|P40998|THI2_SCHPO THIAZOLE BIOSYNTHETIC ENZYME PRECURSOR >gi|632156|pir||S45597 nmt2 protein - fission yeast (Schizosaccharomyces pombe) >gi|563621|emb|CAA57779.1| (X82363) nmt2 [Schizosaccharomyces pombe] Length = 301 Score = 151 bits (377), Expect = 9e-36 Identities = 96/246 (39%), Positives = 137/246 (55%), Gaps = 19/246 (7%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLA--KGGAKVAIFEKKLSI 59 +RE T+SRA+ Y+ DL E D+ IVGAG +G+ AAYY+ + K+AI E ++ Sbjct: 54 IRESTVSRAMTRRYFSDLDKYAESDIVIVGAGSAGLTAAYYIGTRRPDLKIAIIEASVAP 113 Query: 60 GGGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVA---DAIEVATTIASKTVKA 116 GGG W GG F+ +VV++ A L+E + YE ++G YV A+ +T +A Sbjct: 114 GGGAWLGGQLFSAMVVRKPADLFLNEIGVPYE--DEGDYVVVKHAALFTSTVMARTLALP 171 Query: 117 GVKIFNMIEVEDLVVKD-----NRVSGIVINWTPV-LMTGLH--VDPLTVEAKYVIDSTG 168 VK+FN VEDL+VK+ R++G+V NWT V L GL +DP T+ A V+ +TG Sbjct: 172 NVKLFNATAVEDLIVKEGKDGKQRIAGVVTNWTLVSLNHGLQSCMDPNTINAHLVVSATG 231 Query: 169 HGAQVAQFLLKR----GLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAG 224 H F +KR L+ + + + E L V+ TREVFPG+ V GM + G Sbjct: 232 HDGPFGAFCVKRLASAQLVSNLHDMRPLDMNRAEDLIVKGTREVFPGMIVGGMELSEFDG 291 Query: 225 APRMGP 230 A RMGP Sbjct: 292 ANRMGP 297 >emb|CAB59856.1| (AJ250427) THI2p [Uromyces fabae] Length = 338 Score = 148 bits (371), Expect = 4e-35 Identities = 94/267 (35%), Positives = 137/267 (51%), Gaps = 17/267 (6%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYL--AKGGAKVAIFEKKLSI 59 ++E + RA++ Y++D+ DV IVGAG +G+ AY L A+ K+ I E ++ Sbjct: 58 IKEHQVQRAMVRRYFQDMEERAISDVIIVGAGSAGLSCAYALGTARPDLKITILESNVAP 117 Query: 60 GGGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTVKA-GV 118 GGG W GG + +V ++ A E LD+ + YE+ V A +T+ SK + V Sbjct: 118 GGGCWLGGQLMSAMVCRKPADEFLDQVGVPYEDEGNFVVVKHAALFTSTVLSKVLAMPNV 177 Query: 119 KIFNMIEVEDLVVKD-------NRVSGIVINWTPVLMTGLH---VDPLTVEAKYVIDSTG 168 K+FN EDL++K R++G V NWT V + H +DP T+ A V G Sbjct: 178 KMFNATACEDLIIKPCPINPGVQRIAGCVTNWTLVSLNHDHQSCMDPSTITAPLVCSFAG 237 Query: 169 HGAQVAQFLLKR----GLIERIPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAG 224 H F +KR GL E + + E+ E TRE+ PGL V GM + G Sbjct: 238 HDGPFGAFCVKRVASAGLSEGLGDMRPLDMERAEDHIANKTREILPGLIVGGMELSEFDG 297 Query: 225 APRMGPIFGGMFLSGKKAAQEILEKLN 251 + RMGP FG M LSGK+AA+ L+ L+ Sbjct: 298 SARMGPTFGAMLLSGKRAAEVALQSLD 324 >gb|AAG20644.1| (AE005133) thiamine biosynthetic enzyme; Thi1 [Halobacterium sp. NRC-1] Length = 266 Score = 142 bits (355), Expect = 3e-33 Identities = 89/246 (36%), Positives = 122/246 (49%), Gaps = 33/246 (13%) Query: 38 VAAYYLAKGGAKVAIFEKKLSIGGGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEK-- 95 +AA LA V I EK +GGG W GG NK+ V+ A +LD+ + YE E+ Sbjct: 1 MAAKELADRDVDVTIIEKNNYLGGGFWLGGFLMNKLTVRSPAEAVLDDLGVPYEYDEEND 60 Query: 96 GYYVADAIEVATTIASKTVKAGVKIFNMIEVEDLVVKDNR-VSGIVINWTPVLMTGLH-- 152 G VADA + + + AG +I NM E D+VV+D+ V+G V+NWTPV Sbjct: 61 GLAVADAPHACSAMITAACDAGARIQNMTEFTDIVVRDDHAVAGAVVNWTPVHSLPRELT 120 Query: 153 -VDPLTVEAKYVIDSTGHGAQVAQFLLKRGLIER-------------------------- 185 VDP+ +EA V+D+TGH A V L +RG++E Sbjct: 121 CVDPIALEADVVVDATGHDAVVVSKLHERGVLEADGIEHVEEHATGMDQSGDGEYGAPGH 180 Query: 186 -IPGEGAMWAEQGERLTVENTREVFPGLYVTGMAANAIAGAPRMGPIFGGMFLSGKKAAQ 244 PG +MW E VE T +V GL G++ + G RMGP FG M LSGK AA Sbjct: 181 DSPGHDSMWVADSEDKVVEQTGKVHDGLVTAGLSTATVHGLTRMGPTFGAMLLSGKVAAN 240 Query: 245 EILEKL 250 ++++L Sbjct: 241 AVMDEL 246 >gi|6321583 component of the biosynthetic pathway producing the thiazole precursor of thiamine; Thi4p [Saccharomyces cerevisiae] >gi|417307|sp|P32318|THI4_YEAST THIAZOLE BIOSYNTHETIC ENZYME PRECURSOR >gi|283204|pir||S25321 thiamin biosynthesis protein thi4 - yeast (Saccharomyces cerevisiae) >gi|4589|emb|CAA43843.1| (X61669) ESP35 protein [Saccharomyces cerevisiae] >gi|1323242|emb|CAA97157.1| (Z72929) ORF YGR144w [Saccharomyces cerevisiae] Length = 326 Score = 122 bits (304), Expect = 3e-27 Identities = 96/282 (34%), Positives = 138/282 (48%), Gaps = 37/282 (13%) Query: 2 LREVTISRAIIESYYRDLLNNLELDVAIVGAGPSGMVAAYYLAKG--GAKVAIFEKKLSI 59 +RE T+SRA+ Y++DL DV IVGAG SG+ AAY +AK KV I E ++ Sbjct: 43 IRESTVSRAMTSRYFKDLDKFAVSDVIIVGAGSSGLSAAYVIAKNRPDLKVCIIESSVAP 102 Query: 60 GGGIWGGGMGFNKVVVQEEAREILDEFDIRYEEFEKGYYVADAIEVATTIASKTVK-AGV 118 GGG W GG F+ +V+++ A L E +I YE+ V A +T+ SK ++ V Sbjct: 103 GGGSWLGGQLFSAMVMRKPAHLFLQELEIPYEDEGDYVVVKHAALFISTVLSKVLQLPNV 162 Query: 119 KIFNMIEVEDLVVK------DNRVSGIVINWTPVLMTGLH-----VDPLTVE-AKY---- 162 K+FN VEDLV + + V+G+V NWT L+T H +DP +E A Y Sbjct: 163 KLFNATCVEDLVTRPPTEKGEVTVAGVVTNWT--LVTQAHGTQCCMDPNVIELAGYKNDG 220 Query: 163 ----------VIDSTGHGAQVAQFLLKR----GLIERIPGEGAMWAEQGERLTVENTREV 208 ++ +TGH F KR +++ G + E V ++ Sbjct: 221 TRDLSQKHGVILSTTGHDGPFGAFCAKRIVDIDQNQKLGGMKGLDMNHAEHDVVIHSGAY 280 Query: 209 --FPGLYVTGMAANAIAGAPRMGPIFGGMFLSGKKAAQEILE 248 +Y GM + G RMGP FG M LSG AA++IL+ Sbjct: 281 AGVDNMYFAGMEVAELDGLNRMGPTFGAMALSGVHAAEQILK 322 >emb|CAB64776.1| (AJ250474) thiazole biosynthetic enzyme [Brassica juncea] Length = 134 Score = 74.9 bits (181), Expect = 9e-13 Identities = 46/102 (45%), Positives = 57/102 (55%), Gaps = 4/102 (3%) Query: 153 VDPLTVEAKYVIDSTGH----GAQVAQFLLKRGLIERIPGEGAMWAEQGERLTVENTREV 208 +DP +EAK V+ S GH GA + L GLI+ +PG A+ E V TREV Sbjct: 8 MDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGLIDHVPGMKALDMNTVEDAIVRLTREV 67 Query: 209 FPGLYVTGMAANAIAGAPRMGPIFGGMFLSGKKAAQEILEKL 250 + VTGM I GAPRMGP FG M +SG+KAA L+ L Sbjct: 68 DLDMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKAL 109 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.319 0.139 0.394 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 90761748 Number of Sequences: 2977 Number of extensions: 3856223 Number of successful extensions: 11600 Number of sequences better than 1.0e-10: 27 Number of HSP's better than 0.0 without gapping: 15 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 11497 Number of HSP's gapped (non-prelim): 28 length of query: 252 length of database: 189,106,746 effective HSP length: 56 effective length of query: 196 effective length of database: 155,591,474 effective search space: 30495928904 effective search space used: 30495928904 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.7 bits) S2: 164 (68.3 bits)