BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0576 (PAB0576) DE:PUTATIVE HYDROGENASE MATURATION PROTEASE
(147 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||D75132 probable hydrogenase maturation proteinase PAB0576 -... 296 1e-79
pir||F71089 probable hydrogenase - Pyrococcus horikoshii >gi|325... 247 4e-65
sp|Q58048|Y631_METJA PUTATIVE HYDROGENASE MATURATION PROTEASE MJ... 104 4e-22
pir||E69198 coenzyme F420-reducing hydrogenase, delta subunit ho... 93 2e-18
sp|Q57451|HYCI_ECOLI HYDROGENASE 3 MATURATION PROTEASE >gi|21211... 83 1e-15
prf||2104213J protease HycI [Escherichia coli] 83 1e-15
sp|Q60339|FRHD_METJA COENZYME F420 HYDROGENASE DELTA SUBUNIT (PU... 70 1e-11
>pir||D75132 probable hydrogenase maturation proteinase PAB0576 - Pyrococcus
abyssi (strain Orsay) >gi|5458284|emb|CAB49773.1|
(AJ248285) PUTATIVE HYDROGENASE MATURATION PROTEASE
[Pyrococcus abyssi]
Length = 147
Score = 296 bits (749), Expect = 1e-79
Identities = 147/147 (100%), Positives = 147/147 (100%)
Query: 1 MRVVICCIGNELKGDDAFGILVYERLKYIIKDKAIIINCGNVPENYLGKIINANPDLVIL 60
MRVVICCIGNELKGDDAFGILVYERLKYIIKDKAIIINCGNVPENYLGKIINANPDLVIL
Sbjct: 1 MRVVICCIGNELKGDDAFGILVYERLKYIIKDKAIIINCGNVPENYLGKIINANPDLVIL 60
Query: 61 VDAVHFGGKVGELVIVDPEETLGEAFSTHSLPLKFLTRFIKENTNAKVLLLGCQPRSVEF 120
VDAVHFGGKVGELVIVDPEETLGEAFSTHSLPLKFLTRFIKENTNAKVLLLGCQPRSVEF
Sbjct: 61 VDAVHFGGKVGELVIVDPEETLGEAFSTHSLPLKFLTRFIKENTNAKVLLLGCQPRSVEF 120
Query: 121 LGEVSEEVKESVEKAVNYIIKLLHNSV 147
LGEVSEEVKESVEKAVNYIIKLLHNSV
Sbjct: 121 LGEVSEEVKESVEKAVNYIIKLLHNSV 147
>pir||F71089 probable hydrogenase - Pyrococcus horikoshii
>gi|3257393|dbj|BAA30076.1| (AP000004) 147aa long
hypothetical hydrogenase [Pyrococcus horikoshii]
Length = 147
Score = 247 bits (625), Expect = 4e-65
Identities = 120/147 (81%), Positives = 135/147 (91%)
Query: 1 MRVVICCIGNELKGDDAFGILVYERLKYIIKDKAIIINCGNVPENYLGKIINANPDLVIL 60
M+VVICCIGNELKGDDAFGILVYE LK I+D+A++INCGNVPENY+GKII+A+PDLVIL
Sbjct: 1 MKVVICCIGNELKGDDAFGILVYEMLKGKIRDEALLINCGNVPENYIGKIIDADPDLVIL 60
Query: 61 VDAVHFGGKVGELVIVDPEETLGEAFSTHSLPLKFLTRFIKENTNAKVLLLGCQPRSVEF 120
VDAVHFGGK GELVIVDPEETLGEAFSTHSLPLK LT+FI+ENT AKVLLLGCQPR+VEF
Sbjct: 61 VDAVHFGGKPGELVIVDPEETLGEAFSTHSLPLKVLTKFIRENTRAKVLLLGCQPRNVEF 120
Query: 121 LGEVSEEVKESVEKAVNYIIKLLHNSV 147
LG VSEEVK + E+A NYI++LL N V
Sbjct: 121 LGVVSEEVKAAAERATNYIVRLLGNYV 147
>sp|Q58048|Y631_METJA PUTATIVE HYDROGENASE MATURATION PROTEASE MJ0631
>gi|2127981|pir||G64378 hydrogenase maturation
proteinase homolog - Methanococcus jannaschii
>gi|1591342|gb|AAB98623.1| (U67510) hydrogenase
maturation protease (hycI) [Methanococcus jannaschii]
Length = 169
Score = 104 bits (258), Expect = 4e-22
Identities = 56/150 (37%), Positives = 94/150 (62%), Gaps = 9/150 (6%)
Query: 2 RVVICCIGNELKGDDAFGILVYERL-KYIIKDKAII-------INCGNVPENYLGKIINA 53
++VI IGNELKGDDA GI V ++L +Y ++K I IN G VP+ + +
Sbjct: 14 KLVIMGIGNELKGDDAVGIYVVKKLMRYFGEEKEFINIKNLYLINAGTVPDFFTDILKEI 73
Query: 54 NPDLVILVDAVHFGGKVGELVIVDPEETLGEAFSTHSLPLKFLTRFIKENTNAKVLLLGC 113
P ++++D VGE+ I+ +E + +FSTH+LPL + +++K+ NA++++LG
Sbjct: 74 KPTHILIIDCALMDKDVGEVKIIKEDEIINYSFSTHTLPLSIIVKYLKKFINAEIIILGI 133
Query: 114 QPRSVEFLGEVSEEVKESVEKAVNYIIKLL 143
QP+ ++F +SEEVK S +K VN +I+++
Sbjct: 134 QPKIIDFC-PISEEVKLSGDKLVNTLIEII 162
>pir||E69198 coenzyme F420-reducing hydrogenase, delta subunit homolog -
Methanobacterium thermoautotrophicum (strain Delta H)
>gi|2621826|gb|AAB85241.1| (AE000853) coenzyme
F420-reducing hydrogenase, delta subunit homolog
[Methanobacterium thermoautotrophicum]
Length = 168
Score = 92.8 bits (227), Expect = 2e-18
Identities = 44/143 (30%), Positives = 85/143 (58%), Gaps = 1/143 (0%)
Query: 2 RVVICCIGNELKGDDAFGILVYERLKYIIKDKA-IIINCGNVPENYLGKIINANPDLVIL 60
R+++ +GNEL+ DD G + ++ ++++ ++IN G VPEN+ GKI + P +++
Sbjct: 19 RLLVLTVGNELRSDDGLGPYLASLIQEAMEERGHLVINAGTVPENFTGKIRSERPSHILI 78
Query: 61 VDAVHFGGKVGELVIVDPEETLGEAFSTHSLPLKFLTRFIKENTNAKVLLLGCQPRSVEF 120
VDAV + G + +++ + + STH++PL FL R++++ ++ L+G QP ++EF
Sbjct: 79 VDAVEMREEPGTVRLIERDSISEYSISTHAMPLSFLVRYLEDQGAYRIALMGVQPENLEF 138
Query: 121 LGEVSEEVKESVEKAVNYIIKLL 143
+S V+++V II L
Sbjct: 139 GTGLSPAVEDAVHDLAGMIISAL 161
>sp|Q57451|HYCI_ECOLI HYDROGENASE 3 MATURATION PROTEASE >gi|2121160|pir||S67469
hydrogenase 3 hycI - Escherichia coli
>gi|882610|gb|AAA69227.1| (U29579) hycI gene product
[Escherichia coli] >gi|1136794|emb|CAA35554.1| (X17506)
hycI [Escherichia coli] >gi|1789072|gb|AAC75759.1|
(AE000356) protease involved in processing C-terminal
end of the large subunit of hydrogenase 3 [Escherichia
coli K12]
Length = 156
Score = 83.5 bits (203), Expect = 1e-15
Identities = 47/135 (34%), Positives = 79/135 (57%), Gaps = 4/135 (2%)
Query: 1 MRVVICCIGNELKGDDAFGILVYERLKYIIKDKAIIINCGNVPENYLGKIINANPDLVIL 60
M V+ C+GN + GDD G L+ E+ K ++I+ G+ PEN + I P +++
Sbjct: 1 MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLI 60
Query: 61 VDAVHFGGKVGELVIVDPEETLGEAF--STHSLPLKFLTRFIKENTNAKVLLLGCQPRSV 118
VDA G GE+ I+DP++ + E F +TH++PL +L +KE+ +V+ LG QP V
Sbjct: 61 VDATDMGLNPGEIRIIDPDD-IAEMFMMTTHNMPLNYLIDQLKEDI-GEVIFLGIQPDIV 118
Query: 119 EFLGEVSEEVKESVE 133
F +++ +K++VE
Sbjct: 119 GFYYPMTQPIKDAVE 133
>prf||2104213J protease HycI [Escherichia coli]
Length = 156
Score = 83.1 bits (202), Expect = 1e-15
Identities = 46/132 (34%), Positives = 78/132 (58%), Gaps = 4/132 (3%)
Query: 4 VICCIGNELKGDDAFGILVYERLKYIIKDKAIIINCGNVPENYLGKIINANPDLVILVDA 63
V+ C+GN + GDD G L+ E+ K ++I+ G+ PEN + I P +++VDA
Sbjct: 4 VLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLIVDA 63
Query: 64 VHFGGKVGELVIVDPEETLGEAF--STHSLPLKFLTRFIKENTNAKVLLLGCQPRSVEFL 121
G GE+ I+DP++ + E F +TH++PL +L +KE+ +V+ LG QP V F
Sbjct: 64 TDMGLNPGEIRIIDPDD-IAEMFMMTTHNMPLNYLIDQLKEDI-GEVIFLGIQPDIVGFY 121
Query: 122 GEVSEEVKESVE 133
+++ +K++VE
Sbjct: 122 YPMTQPIKDAVE 133
>sp|Q60339|FRHD_METJA COENZYME F420 HYDROGENASE DELTA SUBUNIT (PUTATIVE COENZYME F420
HYDROGENASE PROCESSING SUBUNIT) >gi|2127828|pir||F64303
coenzyme F420 hydrogenase deleta chain [similarity] -
Methanococcus jannaschii >gi|1590832|gb|AAB98009.1|
(U67461) coenzyme F420-reducing hydrogenase, delta
subunit (frcD) [Methanococcus jannaschii]
Length = 183
Score = 70.2 bits (169), Expect = 1e-11
Identities = 48/154 (31%), Positives = 83/154 (53%), Gaps = 14/154 (9%)
Query: 3 VVICCIGNELKGDDAFGILVYERLKYIIKDK-----AIIINCGNVPENYLGKI-INANPD 56
+++ GN L DD F + V E+L I+ DK A++ P+ L I N+
Sbjct: 28 IMVLACGNILFADDGFSVHVIEKLNKILTDKEKQKIALVDAGAGAPQQVLTLIDENSKTK 87
Query: 57 LVILVDAVHFGGKVGELVIVDPEETLGEAF---STHSLPLKFLTRFIKENTNAKVLLLGC 113
+I+VD + +G K GE+ I++ +E + +H PL L R + E N +V ++GC
Sbjct: 88 KIIVVDVIDWGIKPGEIKIIEKDELPNPKYHRLDSHDWPLAPLLREVAEKYNIEVKVVGC 147
Query: 114 QPRSVE----FLGEVSEEVKESVEKAVNYIIKLL 143
Q + + ++G +SEEV+++V+KAV I++ L
Sbjct: 148 QAKYISEPDVYIG-LSEEVEKAVDKAVEIILREL 180
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.321 0.143 0.408
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53884647
Number of Sequences: 2977
Number of extensions: 2153005
Number of successful extensions: 5144
Number of sequences better than 1.0e-10: 7
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 5133
Number of HSP's gapped (non-prelim): 7
length of query: 147
length of database: 189,106,746
effective HSP length: 52
effective length of query: 95
effective length of database: 157,985,422
effective search space: 15008615090
effective search space used: 15008615090
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 161 (67.1 bits)