BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0601 (ubiE) DE:ubiquinone/menaquinone biosynthesis
methyltransferase (ubiE)
(200 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||B75137 ubiquinone/menaquinone biosynthesis methyl transfera... 400 e-111
pir||F71102 hypothetical protein PH1081 - Pyrococcus horikoshii ... 212 2e-54
pir||E72392 ubiquinone/menaquinone biosynthesis-related protein ... 146 2e-34
gi|11497757 ubiquinone/menaquinone biosynthesis methyltransferas... 141 7e-33
sp|Q05197|PMTA_RHOSH PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERAS... 87 1e-16
dbj|BAA34057.1| (AB019196) phosphatidylethanolamine N-methyltran... 80 2e-14
pir||B83014 ubiquinone biosynthesis methyltransferase UbiE PA506... 80 3e-14
gb|AAD02212.1| (AF042276) o251 homolog [Pseudomonas putida] 78 6e-14
emb|CAA09105.1| (AJ010393) hypothetical protein [Pseudomonas ole... 75 5e-13
pir||C83545 probable methyltransferase PA0798 [imported] - Pseud... 75 5e-13
sp|Q9ZCP3|UBIE_RICPR PROBABLE UBIQUINONE/MENAQUINONE BIOSYNTHESI... 75 6e-13
gb|AAF20268.1|AF113007_1 (AF113007) PRO0066 [Homo sapiens] 75 8e-13
dbj|BAB14643.1| (AK023693) unnamed protein product [Homo sapiens] 75 8e-13
pir||T08785 hypothetical protein DKFZp586A0522.1 - human (fragme... 75 8e-13
dbj|BAB14913.1| (AK024409) unnamed protein product [Homo sapiens] 75 8e-13
gb|AAF33422.1| (AF233324) 96% identity with E. coli ubiquinone b... 74 1e-12
pir||E69758 hypothetical protein ycgJ - Bacillus subtilis >gi|26... 73 2e-12
pir||D82366 ubiquinone/menaquinone biosynthesis methlytransferas... 73 2e-12
emb|CAC01471.1| (AL391014) conserved hypothetical protein [Strep... 72 4e-12
gb|AAA67628.1| (M87049) o251 [Escherichia coli] 72 6e-12
pir||S30722 hypothetical protein o251 - Escherichia coli 72 6e-12
sp|P27851|UBIE_ECOLI UBIQUINONE/MENAQUINONE BIOSYNTHESIS METHYLT... 71 1e-11
pir||S62195 hypothetical protein 3 - Methanosarcina barkeri >gi|... 69 3e-11
sp|O86169|MENH_BACST 2-HEPTAPRENYL-1,4-NAPHTHOQUINONE METHYLTRAN... 68 8e-11
>pir||B75137 ubiquinone/menaquinone biosynthesis methyl transferase (ubie)
PAB0601 - Pyrococcus abyssi (strain Orsay)
>gi|5458322|emb|CAB49811.1| (AJ248285)
ubiquinone/menaquinone biosynthesis methyl transferase
(ubiE) [Pyrococcus abyssi]
Length = 200
Score = 400 bits (1016), Expect = e-111
Identities = 200/200 (100%), Positives = 200/200 (100%)
Query: 1 MSYREKYNRIGSRYDILEVPLNRYFEPLREKAVKRVSGKVLEIGVGTGKTLKYYPRNVEL 60
MSYREKYNRIGSRYDILEVPLNRYFEPLREKAVKRVSGKVLEIGVGTGKTLKYYPRNVEL
Sbjct: 1 MSYREKYNRIGSRYDILEVPLNRYFEPLREKAVKRVSGKVLEIGVGTGKTLKYYPRNVEL 60
Query: 61 YAIDGSEEMLKVARERAKSLGINAKFIRAEAENLPFPNDFFDYVVSSFVFCTVPNPERAM 120
YAIDGSEEMLKVARERAKSLGINAKFIRAEAENLPFPNDFFDYVVSSFVFCTVPNPERAM
Sbjct: 61 YAIDGSEEMLKVARERAKSLGINAKFIRAEAENLPFPNDFFDYVVSSFVFCTVPNPERAM 120
Query: 121 KEIVRVLKPGGGAIFLEHTLSDSTVLNLFFLMPLELILSPLLDDSTTRETHKLVRKFLEV 180
KEIVRVLKPGGGAIFLEHTLSDSTVLNLFFLMPLELILSPLLDDSTTRETHKLVRKFLEV
Sbjct: 121 KEIVRVLKPGGGAIFLEHTLSDSTVLNLFFLMPLELILSPLLDDSTTRETHKLVRKFLEV 180
Query: 181 EKEESYYRGIVRLIVARKPF 200
EKEESYYRGIVRLIVARKPF
Sbjct: 181 EKEESYYRGIVRLIVARKPF 200
>pir||F71102 hypothetical protein PH1081 - Pyrococcus horikoshii
>gi|3257497|dbj|BAA30180.1| (AP000004) 132aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 132
Score = 212 bits (535), Expect = 2e-54
Identities = 99/129 (76%), Positives = 116/129 (89%)
Query: 69 MLKVARERAKSLGINAKFIRAEAENLPFPNDFFDYVVSSFVFCTVPNPERAMKEIVRVLK 128
MLKVARE+A+ LGIN KF +AEAE+LPFPNDFFD+V+SSFVFCT+PNP++AM+EI+RVLK
Sbjct: 1 MLKVAREKARQLGINVKFFKAEAEDLPFPNDFFDFVISSFVFCTIPNPKKAMREIIRVLK 60
Query: 129 PGGGAIFLEHTLSDSTVLNLFFLMPLELILSPLLDDSTTRETHKLVRKFLEVEKEESYYR 188
P G IFLEHTLSDS ++N+ FL PLELIL PL+DDSTTRETHKLVRKF VE+EESYY+
Sbjct: 61 PSGKVIFLEHTLSDSFLINMLFLAPLELILRPLIDDSTTRETHKLVRKFFRVEREESYYK 120
Query: 189 GIVRLIVAR 197
GIVR IVAR
Sbjct: 121 GIVRFIVAR 129
>pir||E72392 ubiquinone/menaquinone biosynthesis-related protein - Thermotoga
maritima (strain MSB8)
>gi|4980819|gb|AAD35406.1|AE001713_10 (AE001713)
ubiquinone/menaquinone biosynthesis-related protein
[Thermotoga maritima]
Length = 207
Score = 146 bits (365), Expect = 2e-34
Identities = 84/202 (41%), Positives = 124/202 (60%), Gaps = 5/202 (2%)
Query: 1 MSYREKYNRIGSRYDILEVPLNR-YFEPLREKAVKRVSGK-VLEIGVGTGKTLKYYPRNV 58
MS KY+R + YD E + + +F RE+ KRV GK +LE+G+GTGK + YY ++
Sbjct: 1 MSVERKYDRFAALYDKFESFIEKKFFSRFREELFKRVEGKKILEVGIGTGKNVPYYSDDM 60
Query: 59 ELYAIDGSEEMLKVARERAKSLGIN-AKFIRAEAENLPFPNDFFDYVVSSFVFCTVPNPE 117
++ +D SE ML+V +ER K K +RA+ +NLPF + +D VVS+FVFCTVP+P
Sbjct: 61 DVVGVDISEGMLRVCQERLKKFPEKKVKLLRADVQNLPFSDGEYDCVVSTFVFCTVPDPV 120
Query: 118 RAMKEIVRVLKPGGGAIFLEHTLSDSTVLNLFFLMPLELILSPLLDDSTTRETHKLVRKF 177
+ +KE+ RVL+P G A+FLEH S +N+ + + + LL S R+T ++K
Sbjct: 121 KGLKEVHRVLRPSGKAVFLEHMRSRKWYVNVILFL-MHIFTKLLLGTSMLRKTVDNIKKA 179
Query: 178 LEVEKEESY-YRGIVRLIVARK 198
V +EE Y +VRLI+ARK
Sbjct: 180 GFVIEEEKYLLSDVVRLIIARK 201
>gi|11497757 ubiquinone/menaquinone biosynthesis methyltransferase (ubiE)
[Archaeoglobus fulgidus] >gi|7484006|pir||D69267
ubiquinone/menaquinone biosynthesis methyltransferase
(ubiE) homolog - Archaeoglobus fulgidus
>gi|2650497|gb|AAB91083.1| (AE001096)
ubiquinone/menaquinone biosynthesis methyltransferase
(ubiE) [Archaeoglobus fulgidus]
Length = 261
Score = 141 bits (351), Expect = 7e-33
Identities = 86/206 (41%), Positives = 116/206 (55%), Gaps = 15/206 (7%)
Query: 2 SYREKYNRIGSRYDILEVPLN----RYFEPLREKAVKRVSGKVLEIGVGTGKTLKYYPRN 57
S R +YNRI YD+ E P+ R + + +K G VLEIG GTGK + +YP N
Sbjct: 22 SDRRRYNRIARFYDLFETPMELMNFRKWRKMLFDLIKAEKGLVLEIGAGTGKNIPHYPSN 81
Query: 58 VELYAIDGSEEMLKVARERAKSLGINAKFIRAEAENLPFPNDFFDYVVSSFVFCTVPNPE 117
V A+D SE+ML+ A RAK G F+ A+AENLPF ++ FD V ++FVFC+V +P
Sbjct: 82 V--VALDISEKMLERAVRRAKESGKKVDFLLADAENLPFRSNSFDVVFTTFVFCSVDDPV 139
Query: 118 RAMKEIVRVLKPGGGAIFLEHTLSDSTVLNLFFLMPLELILSPLL----DDSTTRETHKL 173
R ++E RVLK GG A FLEH L S FL PL +L+P+ + R +
Sbjct: 140 RGLREAFRVLKKGGRAYFLEHMLPKSR-----FLHPLFNLLNPIFRAFGPEINRRTDENI 194
Query: 174 VRKFLEVEKEESYYRGIVRLIVARKP 199
R ++ KE + + RLIVA KP
Sbjct: 195 QRAGFQIVKEYMLFGSVFRLIVAEKP 220
>sp|Q05197|PMTA_RHOSH PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE >gi|538854|pir||A47149
phosphatidylethanolamine N-methyltransferase (EC
2.1.1.17) [validated] - Rhodobacter sphaeroides
>gi|151985|gb|AAA26152.1| (L07247)
phosphatidylethanolamine N-methyltransferase
[Rhodobacter sphaeroides]
Length = 203
Score = 87.0 bits (212), Expect = 1e-16
Identities = 49/140 (35%), Positives = 75/140 (53%), Gaps = 5/140 (3%)
Query: 8 NRIGSRYDILEVPLNRYFEPLREKAVKRVS---GKVLEIGVGTGKTLKYYPRNVELYAID 64
+R R+ I + + E R + V+ G+VLE+GVGTG +L Y V + ID
Sbjct: 7 SRSYKRWAIYDFSFGKVSESPRRRTAAHVNARGGRVLEVGVGTGLSLPLYSHRVAVTGID 66
Query: 65 GSEEMLKVARERAKSLGINA--KFIRAEAENLPFPNDFFDYVVSSFVFCTVPNPERAMKE 122
S EML ARE+ + +G+ + + +A L FP++ FD VV+ F+ VP PER + E
Sbjct: 67 FSHEMLARAREKVEEMGLEPVKELRQMDARELDFPDETFDTVVAMFLVSVVPEPERVVSE 126
Query: 123 IVRVLKPGGGAIFLEHTLSD 142
+ RV + GG + + H D
Sbjct: 127 MARVCRKGGEVVIVNHFARD 146
>dbj|BAA34057.1| (AB019196) phosphatidylethanolamine N-methyltransferase
[Acetobacter aceti] >gi|4519586|dbj|BAA75664.1|
(AB017506) Phosphatidylethanolamine methyl transferase
[Acetobacter aceti]
Length = 265
Score = 80.0 bits (194), Expect = 2e-14
Identities = 52/146 (35%), Positives = 81/146 (54%), Gaps = 11/146 (7%)
Query: 7 YNRIGSRYDILEVPLNRYFEPLREKAVKRVSG----KVLEIGVGTGKTLKYYPRNVELYA 62
Y R + YD++ ++R+ R +AV+ V+G VLE+GVGTG L +Y ++
Sbjct: 68 YRRWANVYDVVFGGISRFG---RLRAVEAVNGLPGTDVLEVGVGTGLALPHYRPEKQITG 124
Query: 63 IDGSEEMLKVARERA---KSLGINAKFIRAEAENLPFPNDFFDYVVSSFVFCTVPNPERA 119
ID S +ML ARER K +N + +AE+ F FD V+ FV VP+P +
Sbjct: 125 IDLSADMLAKARERVQRDKLTNVNG-LLEMDAEDTKFEVASFDIAVAMFVASVVPHPRQL 183
Query: 120 MKEIVRVLKPGGGAIFLEHTLSDSTV 145
++E+ RV++PGG +F+ H L+ V
Sbjct: 184 LRELKRVVRPGGYILFVNHFLAPGGV 209
>pir||B83014 ubiquinone biosynthesis methyltransferase UbiE PA5063 [imported] -
Pseudomonas aeruginosa (strain PAO1)
>gi|9951355|gb|AAG08448.1|AE004919_9 (AE004919)
ubiquinone biosynthesis methyltransferase UbiE
[Pseudomonas aeruginosa]
Length = 256
Score = 79.6 bits (193), Expect = 3e-14
Identities = 52/167 (31%), Positives = 90/167 (53%), Gaps = 17/167 (10%)
Query: 5 EKYNRIGSRYDILEVPLNRYFEPLREKAVKRVSG-----KVLEIGVGTGKTLKYYPRNV- 58
E ++ + ++YD++ ++ L ++ +SG +VL+I GTG + + R V
Sbjct: 32 EVFHSVAAKYDLMNDLMSGGIHRLWKRFTIELSGVRSGNRVLDIAGGTGDLTRQFSRLVG 91
Query: 59 ---ELYAIDGSEEMLKVARERAKSLGI--NAKFIRAEAENLPFPNDFFDYVVSSFVFCTV 113
E+ D + MLKV R++ G+ N F++A+AE LPFP++ FD V +F V
Sbjct: 92 PTGEVVLADINASMLKVGRDKLLDKGVSGNVSFVQADAEKLPFPDNHFDCVTIAFGLRNV 151
Query: 114 PNPERAMKEIVRVLKPGGGAIFLEHTLSDSTVLN------LFFLMPL 154
+ + A++ ++RVLKPGG + LE + S +L+ F L+PL
Sbjct: 152 THKDEAIRSMLRVLKPGGRLLVLEFSKPSSNLLSKAYDAYSFSLLPL 198
>gb|AAD02212.1| (AF042276) o251 homolog [Pseudomonas putida]
Length = 256
Score = 78.4 bits (190), Expect = 6e-14
Identities = 52/167 (31%), Positives = 90/167 (53%), Gaps = 17/167 (10%)
Query: 5 EKYNRIGSRYDILEVPLNRYFEPLREKAVKRVSG-----KVLEIGVGTGKTLKYYPRNV- 58
E ++ + ++YD++ L+ L ++ +SG +VL+I GTG + R V
Sbjct: 32 EVFHSVAAKYDLMNDVLSGGMHRLWKRFTIELSGVRAGNRVLDIAGGTGDLAAKFSRLVG 91
Query: 59 ---ELYAIDGSEEMLKVARERAKSLGI--NAKFIRAEAENLPFPNDFFDYVVSSFVFCTV 113
++ D +E MLKV R+R G+ N +F++A+AE LPFP++ FD V +F V
Sbjct: 92 PTGQVVLADINESMLKVGRDRLLDRGVAGNIEFVQADAEKLPFPDNHFDCVTIAFGLRNV 151
Query: 114 PNPERAMKEIVRVLKPGGGAIFLEHTLSDSTVLN------LFFLMPL 154
+ + A++ ++RVLKPGG + LE + + +++ F MPL
Sbjct: 152 THKDEAIRSMLRVLKPGGRLLVLEFSKPTNKLMSKAYDAYSFAFMPL 198
>emb|CAA09105.1| (AJ010393) hypothetical protein [Pseudomonas oleovorans]
Length = 170
Score = 75.3 bits (182), Expect = 5e-13
Identities = 46/138 (33%), Positives = 78/138 (56%), Gaps = 11/138 (7%)
Query: 5 EKYNRIGSRYDILEVPLNRYFEPLREKAVKRVSG-----KVLEIGVGTGKTLKYYPRNV- 58
E ++ + ++YD++ L+ L ++ +SG +VL+I GTG + R V
Sbjct: 32 EVFHSVAAKYDLMNDVLSGGMHRLWKRFTIELSGVRAGNRVLDIAGGTGDLAAKFSRLVG 91
Query: 59 ---ELYAIDGSEEMLKVARERAKSLGI--NAKFIRAEAENLPFPNDFFDYVVSSFVFCTV 113
++ D +E MLKV R+R G+ N +F++A+AE LPFP++ FD V +F V
Sbjct: 92 PTGQVVLADINESMLKVGRDRLLDRGVAGNIEFVQADAEKLPFPDNHFDCVTIAFGLRNV 151
Query: 114 PNPERAMKEIVRVLKPGG 131
+ + A++ ++RVLKPGG
Sbjct: 152 THKDEAIRSMLRVLKPGG 169
>pir||C83545 probable methyltransferase PA0798 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9946690|gb|AAG04187.1|AE004515_3 (AE004515) probable
methyltransferase [Pseudomonas aeruginosa]
Length = 216
Score = 75.3 bits (182), Expect = 5e-13
Identities = 44/121 (36%), Positives = 67/121 (55%), Gaps = 3/121 (2%)
Query: 29 REKAVKRVSGKVLEIGVGTGKTLKYYP--RNVELYAIDGSEEMLKVARERAKSLGINAKF 86
R V R G+VLEIG+GTG L +Y + + +D + +M +ARERA +GI +
Sbjct: 39 RSLLVPRAHGRVLEIGLGTGLNLGFYDAAKVSAIVGVDPAAQMQALARERAAQIGIPVEM 98
Query: 87 IRAEAENLPFPNDFFDYVVSSFVFCTVPNPERAMKEIVRVLKPGGGAIFLEH-TLSDSTV 145
+ E + + FD +V +F C++ P A+ E+ RVLK GG +F EH D++V
Sbjct: 99 VALELGEIRAEAESFDTIVCTFTLCSIAAPLPALGEMRRVLKRGGELLFCEHGRAPDASV 158
Query: 146 L 146
L
Sbjct: 159 L 159
>sp|Q9ZCP3|UBIE_RICPR PROBABLE UBIQUINONE/MENAQUINONE BIOSYNTHESIS METHYLTRANSFERASE UBIE
>gi|7445288|pir||C71674 ubiquinone/menaquinone
biosynthesis methlytransferase ubie (ubiE) RP680 -
Rickettsia prowazekii >gi|3861217|emb|CAA15117.1|
(AJ235272) UBIQUINONE/MENAQUINONE BIOSYNTHESIS
METHLYTRANSFERASE UBIE (ubiE) [Rickettsia prowazekii]
Length = 265
Score = 74.9 bits (181), Expect = 6e-13
Identities = 48/157 (30%), Positives = 82/157 (51%), Gaps = 13/157 (8%)
Query: 7 YNRIGSRYDILE----VPLNRYFEPLREKAVKRVSGKVLEIGVGTG-------KTLKYYP 55
++R+ +YD++ + L+R ++ + ++ +L++ G+G K K
Sbjct: 41 FSRVADKYDLMNDLMSIGLHRLWKDEFIMQIPNLNSNILDVASGSGDIALKLAKKAKDRG 100
Query: 56 RNVELYAIDGSEEMLKVARERAKSLGI--NAKFIRAEAENLPFPNDFFDYVVSSFVFCTV 113
N+ L D +EEML A++++ L + N KFI A AE LPF ++ FDY +F V
Sbjct: 101 NNISLILSDINEEMLNNAKKKSIDLNLFQNIKFIVANAEELPFSDNSFDYYTIAFGIRNV 160
Query: 114 PNPERAMKEIVRVLKPGGGAIFLEHTLSDSTVLNLFF 150
P+ +A+KE RVLKP G + LE + +L F+
Sbjct: 161 PDINKALKEAYRVLKPMGKFVCLEFSKVKEGILKDFY 197
>gb|AAF20268.1|AF113007_1 (AF113007) PRO0066 [Homo sapiens]
Length = 194
Score = 74.5 bits (180), Expect = 8e-13
Identities = 52/159 (32%), Positives = 78/159 (48%), Gaps = 10/159 (6%)
Query: 25 FEPLREKAVKRVSGKVLEIGVGTGKTLKYYPRNVELYAIDGSEEMLKVARERAKSLGINA 84
F L+E A +LE+G GTG K+YP + ID + K KS+ N
Sbjct: 9 FSNLQEFAGPSGKLSLLEVGCGTGANFKFYPPGCRVTCIDPNPNFEKFL---IKSIAENR 65
Query: 85 -----KFIRAEAENLPFPND-FFDYVVSSFVFCTVPNPERAMKEIVRVLKPGGGAIFLEH 138
+F+ A EN+ D D VV + V C+V N ER ++E+ RVL+PGG F+EH
Sbjct: 66 HLQFERFVVAAGENMHQVADGSVDVVVCTLVLCSVKNQERILREVCRVLRPGGAFYFMEH 125
Query: 139 TLSDSTVLNLFFLMPLELILSPLLDD-STTRETHKLVRK 176
++ + N F+ L+ L D + TRE+ K + +
Sbjct: 126 VAAECSTWNYFWQQVLDPAWHLLFDGCNLTRESWKALER 164
>dbj|BAB14643.1| (AK023693) unnamed protein product [Homo sapiens]
Length = 244
Score = 74.5 bits (180), Expect = 8e-13
Identities = 52/159 (32%), Positives = 78/159 (48%), Gaps = 10/159 (6%)
Query: 25 FEPLREKAVKRVSGKVLEIGVGTGKTLKYYPRNVELYAIDGSEEMLKVARERAKSLGINA 84
F L+E A +LE+G GTG K+YP + ID + K KS+ N
Sbjct: 59 FSNLQEFAGPSGKLSLLEVGCGTGANFKFYPPGCRVTCIDPNPNFEKFL---IKSIAENR 115
Query: 85 -----KFIRAEAENLPFPND-FFDYVVSSFVFCTVPNPERAMKEIVRVLKPGGGAIFLEH 138
+F+ A EN+ D D VV + V C+V N ER ++E+ RVL+PGG F+EH
Sbjct: 116 HLQFERFVVAAGENMHQVADGSVDVVVCTLVLCSVKNQERILREVCRVLRPGGAFYFMEH 175
Query: 139 TLSDSTVLNLFFLMPLELILSPLLDD-STTRETHKLVRK 176
++ + N F+ L+ L D + TRE+ K + +
Sbjct: 176 VAAECSTWNYFWQQVLDPAWHLLFDGCNLTRESWKALER 214
>pir||T08785 hypothetical protein DKFZp586A0522.1 - human (fragment)
>gi|4884372|emb|CAB43300.1| (AL050159) hypothetical
protein [Homo sapiens]
Length = 243
Score = 74.5 bits (180), Expect = 8e-13
Identities = 52/159 (32%), Positives = 78/159 (48%), Gaps = 10/159 (6%)
Query: 25 FEPLREKAVKRVSGKVLEIGVGTGKTLKYYPRNVELYAIDGSEEMLKVARERAKSLGINA 84
F L+E A +LE+G GTG K+YP + ID + K KS+ N
Sbjct: 58 FSNLQEFAGPSGKLSLLEVGCGTGANFKFYPPGCRVTCIDPNPNFEKFL---IKSIAENR 114
Query: 85 -----KFIRAEAENLPFPND-FFDYVVSSFVFCTVPNPERAMKEIVRVLKPGGGAIFLEH 138
+F+ A EN+ D D VV + V C+V N ER ++E+ RVL+PGG F+EH
Sbjct: 115 HLQFERFVVAAGENMHQVADGSVDVVVCTLVLCSVKNQERILREVCRVLRPGGAFYFMEH 174
Query: 139 TLSDSTVLNLFFLMPLELILSPLLDD-STTRETHKLVRK 176
++ + N F+ L+ L D + TRE+ K + +
Sbjct: 175 VAAECSTWNYFWQQVLDPAWHLLFDGCNLTRESWKALER 213
>dbj|BAB14913.1| (AK024409) unnamed protein product [Homo sapiens]
Length = 244
Score = 74.5 bits (180), Expect = 8e-13
Identities = 52/159 (32%), Positives = 78/159 (48%), Gaps = 10/159 (6%)
Query: 25 FEPLREKAVKRVSGKVLEIGVGTGKTLKYYPRNVELYAIDGSEEMLKVARERAKSLGINA 84
F L+E A +LE+G GTG K+YP + ID + K KS+ N
Sbjct: 59 FSNLQEFAGPSGKLSLLEVGCGTGANFKFYPPGCRVTCIDPNPNFEKFL---IKSIAENR 115
Query: 85 -----KFIRAEAENLPFPND-FFDYVVSSFVFCTVPNPERAMKEIVRVLKPGGGAIFLEH 138
+F+ A EN+ D D VV + V C+V N ER ++E+ RVL+PGG F+EH
Sbjct: 116 HLQFERFVVAAGENMHQVADGSVDVVVCTLVLCSVKNQERILREVCRVLRPGGAFYFMEH 175
Query: 139 TLSDSTVLNLFFLMPLELILSPLLDD-STTRETHKLVRK 176
++ + N F+ L+ L D + TRE+ K + +
Sbjct: 176 VAAECSTWNYFWQQVLDPAWHLLFDGCNLTRESWKALER 214
>gb|AAF33422.1| (AF233324) 96% identity with E. coli ubiquinone biosynthesis
methyltransferase (UBIE) (SP:P27851); contains
similarity to Pfam family PF01209 (ubiE/COQ5
methyltransferase family), score=588.5, E=4.1e-173, N=1
[Salmonella typhimurium LT2]
Length = 251
Score = 73.7 bits (178), Expect = 1e-12
Identities = 44/142 (30%), Positives = 79/142 (54%), Gaps = 11/142 (7%)
Query: 7 YNRIGSRYDILEVPLNRYFEPLREKAVKRVSG-----KVLEIGVGTGKTLKYYPRNV--- 58
++ + S+YD++ ++ L ++ SG VL++ GTG + R V
Sbjct: 29 FHSVASKYDVMNDLMSFGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRMVGET 88
Query: 59 -ELYAIDGSEEMLKVARERAKSLGI--NAKFIRAEAENLPFPNDFFDYVVSSFVFCTVPN 115
++ D ++ MLK+ RE+ +++G+ N ++++A AE LPFP++ FD + SF V
Sbjct: 89 GKVILADINDSMLKMGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTE 148
Query: 116 PERAMKEIVRVLKPGGGAIFLE 137
E+A++ + RVLKPGG + LE
Sbjct: 149 KEKALRSMFRVLKPGGRLLVLE 170
>pir||E69758 hypothetical protein ycgJ - Bacillus subtilis
>gi|2632602|emb|CAB12110.1| (Z99105) ycgJ [Bacillus
subtilis]
Length = 228
Score = 73.0 bits (176), Expect = 2e-12
Identities = 46/138 (33%), Positives = 74/138 (53%), Gaps = 7/138 (5%)
Query: 35 RVSGKVLEIGVGTGKT-LKYYPRNVELYAIDGSEEMLKVARERAKSLGI-NAKFIRAEAE 92
R +VL+IG G G T L + P E +D ++EM++VA A+ G+ N +F + AE
Sbjct: 11 RAEHRVLDIGAGAGHTALAFSPYVQECIGVDATKEMVEVASSFAQEKGVENVRFQQGTAE 70
Query: 93 NLPFPNDFFDYVVSSFVFCTVPNPERAMKEIVRVLKPGGGAIFLEHTLSDSTVLNLFFLM 152
+LPFP+D FD + + + +A++E+ RVLK G + ++H + VL+ F
Sbjct: 71 SLPFPDDSFDIITCRYAAHHFSDVRKAVREVARVLKQDGRFLLVDHYAPEDPVLDEFVNH 130
Query: 153 PLELILSPLLDDSTTRET 170
L+ L D S RE+
Sbjct: 131 -----LNRLRDPSHVRES 143
>pir||D82366 ubiquinone/menaquinone biosynthesis methlytransferase UbiE VC0083
[imported] - Vibrio cholerae (group O1 strain N16961)
>gi|9654480|gb|AAF93261.1| (AE004099)
ubiquinone/menaquinone biosynthesis methlytransferase
UbiE [Vibrio cholerae]
Length = 260
Score = 73.0 bits (176), Expect = 2e-12
Identities = 42/142 (29%), Positives = 77/142 (53%), Gaps = 11/142 (7%)
Query: 7 YNRIGSRYDILEVPLNRYFEPLREKAVKRVSG-----KVLEIGVGTGKTLKYYPRNV--- 58
++ + ++YDI+ ++ L ++ SG ++L++G GTG + R V
Sbjct: 38 FHSVAAKYDIMNDLMSGGIHRLWKRFTIDCSGARPGQRILDLGGGTGDLTAKFSRIVGEK 97
Query: 59 -ELYAIDGSEEMLKVARERAKSLGI--NAKFIRAEAENLPFPNDFFDYVVSSFVFCTVPN 115
+ D + ML V R++ + G+ N +++A AE LPFP+++FD + SF V +
Sbjct: 98 GHVILADINNSMLNVGRDKLRDSGVVGNVHYVQANAEELPFPDNYFDCITISFCLRNVTD 157
Query: 116 PERAMKEIVRVLKPGGGAIFLE 137
++A++ + RVLKPGG + LE
Sbjct: 158 KDKALRSMFRVLKPGGRLLVLE 179
>emb|CAC01471.1| (AL391014) conserved hypothetical protein [Streptomyces coelicolor
A3(2)]
Length = 225
Score = 72.2 bits (174), Expect = 4e-12
Identities = 40/112 (35%), Positives = 61/112 (53%), Gaps = 1/112 (0%)
Query: 28 LREKAVKRVSGKVLEIGVGTGKTLKYYPRNV-ELYAIDGSEEMLKVARERAKSLGINAKF 86
+R + + +SG+V+E+G G G +YP V E+ AI+ + ++A E A +
Sbjct: 41 VRRRLLAGLSGRVIEVGAGNGLNFSHYPGTVSEVVAIEPERVLRQLAVEAALRAEVPVDV 100
Query: 87 IRAEAENLPFPNDFFDYVVSSFVFCTVPNPERAMKEIVRVLKPGGGAIFLEH 138
AE LP ++ FD V S V C+V + RA+ E+ RVL+PGG F EH
Sbjct: 101 APGAAEALPVKSEAFDAAVVSLVLCSVRDVPRALAELRRVLRPGGQVRFFEH 152
>gb|AAA67628.1| (M87049) o251 [Escherichia coli]
Length = 251
Score = 71.8 bits (173), Expect = 6e-12
Identities = 43/142 (30%), Positives = 79/142 (55%), Gaps = 11/142 (7%)
Query: 7 YNRIGSRYDILEVPLNRYFEPLREKAVKRVSG-----KVLEIGVGTGKTLKYYPRNV--- 58
++ + S+YD++ ++ L ++ SG VL++ GTG + R V
Sbjct: 29 FHSVASKYDVMNDLMSFGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGET 88
Query: 59 -ELYAIDGSEEMLKVARERAKSLGI--NAKFIRAEAENLPFPNDFFDYVVSSFVFCTVPN 115
++ D +E MLK+ RE+ +++G+ N ++++A E LPFP++ FD + SF V +
Sbjct: 89 GKVVLADINESMLKMGREKLRNIGVIGNVEYVQAXXEALPFPDNTFDCITISFGLRNVTD 148
Query: 116 PERAMKEIVRVLKPGGGAIFLE 137
++A++ + RVLKPGG + LE
Sbjct: 149 KDKALRSMYRVLKPGGRLLVLE 170
>pir||S30722 hypothetical protein o251 - Escherichia coli
Length = 251
Score = 71.8 bits (173), Expect = 6e-12
Identities = 43/142 (30%), Positives = 79/142 (55%), Gaps = 11/142 (7%)
Query: 7 YNRIGSRYDILEVPLNRYFEPLREKAVKRVSG-----KVLEIGVGTGKTLKYYPRNV--- 58
++ + S+YD++ ++ L ++ SG VL++ GTG + R V
Sbjct: 29 FHSVASKYDVMNDLMSFGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGET 88
Query: 59 -ELYAIDGSEEMLKVARERAKSLGI--NAKFIRAEAENLPFPNDFFDYVVSSFVFCTVPN 115
++ D +E MLK+ RE+ +++G+ N ++++A E LPFP++ FD + SF V +
Sbjct: 89 GKVVLADINESMLKMGREKLRNIGVIGNVEYVQAXXEALPFPDNTFDCITISFGLRNVTD 148
Query: 116 PERAMKEIVRVLKPGGGAIFLE 137
++A++ + RVLKPGG + LE
Sbjct: 149 KDKALRSMYRVLKPGGRLLVLE 170
>sp|P27851|UBIE_ECOLI UBIQUINONE/MENAQUINONE BIOSYNTHESIS METHYLTRANSFERASE UBIE
>gi|7429745|pir||B65188 hypothetical 28.1 kD protein in
udp-rfaH intergenic region - Escherichia coli (strain
K-12) >gi|2367307|gb|AAC76836.1| (AE000459)
2-octaprenyl-6-methoxy-1,4-benzoquinone -->
2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone
[Escherichia coli K12]
Length = 251
Score = 71.0 bits (171), Expect = 1e-11
Identities = 43/142 (30%), Positives = 79/142 (55%), Gaps = 11/142 (7%)
Query: 7 YNRIGSRYDILEVPLNRYFEPLREKAVKRVSG-----KVLEIGVGTGKTLKYYPRNV--- 58
++ + S+YD++ ++ L ++ SG VL++ GTG + R V
Sbjct: 29 FHSVASKYDVMNDLMSFGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGET 88
Query: 59 -ELYAIDGSEEMLKVARERAKSLGI--NAKFIRAEAENLPFPNDFFDYVVSSFVFCTVPN 115
++ D +E M K+ RE+ +++G+ N ++++A AE LPFP++ FD + SF V +
Sbjct: 89 GKVVLADINESMPKMGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTD 148
Query: 116 PERAMKEIVRVLKPGGGAIFLE 137
++A++ + RVLKPGG + LE
Sbjct: 149 KDKALRSMYRVLKPGGRLLVLE 170
>pir||S62195 hypothetical protein 3 - Methanosarcina barkeri
>gi|1124958|emb|CAA63626.1| (X93084) orf3
[Methanosarcina barkeri] >gi|1587234|prf||2206350B ORF 3
[Methanosarcina barkeri]
Length = 262
Score = 69.5 bits (167), Expect = 3e-11
Identities = 36/108 (33%), Positives = 63/108 (58%), Gaps = 1/108 (0%)
Query: 39 KVLEIGVGTGK-TLKYYPRNVELYAIDGSEEMLKVARERAKSLGINAKFIRAEAENLPFP 97
K+L++G GTG+ +L + E+ ID S +MLK+A+ +A++LG + F +AEN PF
Sbjct: 61 KILDVGCGTGELSLLFAEMGHEVAGIDISGQMLKIAKAKAEALGADITFREGDAENPPFD 120
Query: 98 NDFFDYVVSSFVFCTVPNPERAMKEIVRVLKPGGGAIFLEHTLSDSTV 145
+D V S + T+PNP+ A++ R+L+ G + ++ T+
Sbjct: 121 TSSYDIVFSRHLLWTLPNPKVAVENWNRILRKNGKVVIVDGVWDSGTL 168
>sp|O86169|MENH_BACST 2-HEPTAPRENYL-1,4-NAPHTHOQUINONE METHYLTRANSFERASE (MENAQUINONE
BIOSYNTHESIS METHYLTRANSFERASE) >gi|11278758|pir||T48892
2-heptaprenyl-1,4-naphthoquinone methyltransferase
[validated] - Bacillus stearothermophilus
>gi|3452684|dbj|BAA32500.1| (D87054)
2-heptaprenyl-1,4-naphthoquinone methyltransferase
[Bacillus stearothermophilus]
Length = 234
Score = 67.9 bits (163), Expect = 8e-11
Identities = 43/103 (41%), Positives = 57/103 (54%), Gaps = 7/103 (6%)
Query: 59 ELYAIDGSEEMLKVARERAKSLGI-NAKFIRAEAENLPFPNDFFDYVVSSFVFCTVPNPE 117
++Y +D SE MLKV ++ K+ G+ N K I A LPFP++ FDYV F VP+
Sbjct: 74 KVYGLDFSENMLKVGEQKVKARGLHNVKLIHGNAMQLPFPDNSFDYVTIGFGLRNVPDYM 133
Query: 118 RAMKEIVRVLKPGGGAIFLE---HTLSDSTVLNLF---FLMPL 154
+KE+ RV KPGG + LE TL L F F+MPL
Sbjct: 134 TVLKEMHRVTKPGGITVCLETSQPTLFGFRQLYYFYFRFIMPL 176
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.321 0.141 0.398
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 72779099
Number of Sequences: 2977
Number of extensions: 2952700
Number of successful extensions: 7258
Number of sequences better than 1.0e-10: 24
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 20
Number of HSP's that attempted gapping in prelim test: 7232
Number of HSP's gapped (non-prelim): 24
length of query: 200
length of database: 189,106,746
effective HSP length: 54
effective length of query: 146
effective length of database: 156,788,448
effective search space: 22891113408
effective search space used: 22891113408
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 163 (67.9 bits)