BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0608 (metE-like1) DE:related
5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase,
putative
(338 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||C75138 probable 5-methyltetrahydropteroyltriglutamate- homo... 695 0.0
pir||F71103 probable 5-methyltetrahydropteroyltriglutamate--homo... 641 0.0
gb|AAK40736.1| 5-methyltetrahydropteroyltriglutamate- homocystei... 363 1e-99
pir||B72509 probable 5-methltetrahydropteroyltriglutamate--homoc... 230 2e-59
emb|CAC12106.1| (AL445066) methionine synthase (cobalamin-indepe... 223 2e-57
dbj|BAB04157.1| (AP001508) homosystein methyl transferase [Bacil... 154 1e-36
sp|O67606|METE_AQUAE 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMO... 153 2e-36
sp|Q9X112|METE_THEMA 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMO... 152 4e-36
sp|P80877|METE_BACSU 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMO... 148 7e-35
gb|AAF81245.1| (AF263012) 5-methyltetrahydropteroyltriglutamate-... 148 1e-34
gb|AAF00639.1|AC009540_16 (AC009540) putative methionine synthas... 141 1e-32
pir||C81326 5-methyltetrahydropteroyltriglutamate--homocysteine ... 141 1e-32
sp|P93263|METE_MESCR 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMO... 140 2e-32
pir||D83404 5-methyltetrahydropteroyltriglutamate- homocysteine ... 140 2e-32
sp|P57142|METE_BUCAI 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMO... 140 2e-32
sp|Q42699|METE_CATRO 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMO... 138 7e-32
emb|CAA89019.1| (Z49150) cobalamine-independent methionine synth... 138 1e-31
sp|Q42662|METE_SOLSC 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMO... 138 1e-31
sp|O50008|METE_ARATH 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMO... 137 2e-31
gb|AAF74983.1|AF082893_1 (AF082893) methionine synthase [Solanum... 137 2e-31
pir||E82167 5-methyltetrahydropteroyltriglutamate-- homocysteine... 136 3e-31
gi|6320936 vitamin B12-(cobalamin)-independent isozyme of methio... 136 5e-31
gb|AAA65711.1| (U15099) methionine synthase [Saccharomyces cerev... 136 5e-31
pir||F82578 5-methyltetrahydropteroyltriglutamate-- homocysteine... 134 1e-30
pir||E81140 5-methyltetrahydropteroyltriglutamate- homocysteine ... 132 4e-30
sp|O05564|METE_MYCLE 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMO... 132 6e-30
sp|P45331|METE_HAEIN 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMO... 131 1e-29
gb|AAA23544.1| (M87625) cobalamin-independent methionine synthas... 131 2e-29
sp|P25665|METE_ECOLI 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMO... 131 2e-29
gb|AAF33427.1| (AF233324) 94% identity with E. coli 5-methyltetr... 131 2e-29
pir||G81880 probable 5-methyltetrahydropteroyltriglutamate--homo... 130 2e-29
gb|AAA67625.1| (M87049) 5-methyltetrahydropteroyltriglutamate- h... 130 2e-29
sp|O06584|METE_MYCTU 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMO... 130 2e-29
gb|AAF82115.1|AF275676_1 (AF275676) cobalamin-independent methio... 129 5e-29
sp|O26869|Y775_METTH HYPOTHETICAL PROTEIN MTH775 >gi|7447490|pir... 128 8e-29
gb|AAF33834.1| (AF226997) methionine synthase [Cladosporium fulvum] 122 5e-27
sp|Q39586|METE_CHLRE 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMO... 122 8e-27
sp|Q58868|YE73_METJA HYPOTHETICAL PROTEIN MJ1473 >gi|2127725|pir... 121 1e-26
sp|P55299|Y775_METTM HYPOTHETICAL 34.6 KDA PROTEIN IN TYSY 5'REG... 118 9e-26
pir||T39194 5-methyltetrahydropteroyltriglutamate--homocysteine ... 118 9e-26
pir||S47051 hypothetical protein 1 - Xanthobacter sp >gi|520948|... 114 1e-24
pir||T42529 probable 5-methyltetrahydropteroyltriglutamate--homo... 111 9e-24
gb|AAD00267.1| (U77388) cobalamin independent methionine synthas... 98 1e-19
gb|AAF26735.1| (AF220054) methionine synthase [Coffea arabica] 88 2e-16
pir||B75138 tetrahydropteroyltriglutamate methyltransferase PAB2... 80 5e-14
pir||G71103 hypothetical protein PH1090 - Pyrococcus horikoshii ... 71 1e-11
>pir||C75138 probable 5-methyltetrahydropteroyltriglutamate- homocysteine
methyltransferase PAB0608 - Pyrococcus abyssi (strain
Orsay) >gi|5458331|emb|CAB49820.1| (AJ248285) related
5-methyltetrahydropteroyltriglutamate-homocysteine
methyltransferase, putative [Pyrococcus abyssi]
Length = 338
Score = 695 bits (1774), Expect = 0.0
Identities = 338/338 (100%), Positives = 338/338 (100%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP
Sbjct: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
Query: 61 WDGEMWRSEMTEHFTAKISGFKFYGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVRE 120
WDGEMWRSEMTEHFTAKISGFKFYGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVRE
Sbjct: 61 WDGEMWRSEMTEHFTAKISGFKFYGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVRE 120
Query: 121 NTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDEP 180
NTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDEP
Sbjct: 121 NTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDEP 180
Query: 181 AMLNHPDEVPLAVEAINRAVKGIKIKVGLHVCYSNYYLLADYFDDIRVTQFALEFANRQF 240
AMLNHPDEVPLAVEAINRAVKGIKIKVGLHVCYSNYYLLADYFDDIRVTQFALEFANRQF
Sbjct: 181 AMLNHPDEVPLAVEAINRAVKGIKIKVGLHVCYSNYYLLADYFDDIRVTQFALEFANRQF 240
Query: 241 RDMDFLKKLSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPEWLYINPDCGLKLLD 300
RDMDFLKKLSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPEWLYINPDCGLKLLD
Sbjct: 241 RDMDFLKKLSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPEWLYINPDCGLKLLD 300
Query: 301 RRIAYQKLVNMVKAVRIVRKELEKEGRAETEFRTLNDI 338
RRIAYQKLVNMVKAVRIVRKELEKEGRAETEFRTLNDI
Sbjct: 301 RRIAYQKLVNMVKAVRIVRKELEKEGRAETEFRTLNDI 338
>pir||F71103 probable 5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase - Pyrococcus horikoshii
>gi|3257505|dbj|BAA30188.1| (AP000004) 338aa long
hypothetical
5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase [Pyrococcus horikoshii]
Length = 338
Score = 641 bits (1635), Expect = 0.0
Identities = 304/338 (89%), Positives = 323/338 (94%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
MELPILPTSVIGSYPKPRWLLRMY+LR+LGKIPEEDFKEAVKDASV+VLREHERAGVDIP
Sbjct: 1 MELPILPTSVIGSYPKPRWLLRMYRLRDLGKIPEEDFKEAVKDASVSVLREHERAGVDIP 60
Query: 61 WDGEMWRSEMTEHFTAKISGFKFYGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVRE 120
WDGEMWR+EMTEHFTAKI GFKFYGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWV++
Sbjct: 61 WDGEMWRTEMTEHFTAKIGGFKFYGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVKK 120
Query: 121 NTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDEP 180
NTTRE+VK+PITGPYTIAEWSFNEYYPDKESF+MDLAKIINKELK LE HGA YIQLDEP
Sbjct: 121 NTTREVVKIPITGPYTIAEWSFNEYYPDKESFIMDLAKIINKELKMLENHGALYIQLDEP 180
Query: 181 AMLNHPDEVPLAVEAINRAVKGIKIKVGLHVCYSNYYLLADYFDDIRVTQFALEFANRQF 240
AMLNHPDEVPLAVEAIN+AVKGIKIKVGLHVCYSNYYLLADYFD+IRVTQFALEFANRQF
Sbjct: 181 AMLNHPDEVPLAVEAINKAVKGIKIKVGLHVCYSNYYLLADYFDEIRVTQFALEFANRQF 240
Query: 241 RDMDFLKKLSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPEWLYINPDCGLKLLD 300
RDMDFLKKLS KELGFGVVDVHNPRIE+V+EIV+AIKK F Y+EPE LYINPDCG+KLLD
Sbjct: 241 RDMDFLKKLSNKELGFGVVDVHNPRIESVEEIVKAIKKVFEYVEPELLYINPDCGMKLLD 300
Query: 301 RRIAYQKLVNMVKAVRIVRKELEKEGRAETEFRTLNDI 338
R IAY KLVNMVKA +VRKELE+EG+ TEFRTL DI
Sbjct: 301 RNIAYNKLVNMVKATELVRKELEREGKKTTEFRTLKDI 338
>gb|AAK40736.1| 5-methyltetrahydropteroyltriglutamate- homocysteine
methyltransferase (metE-2) [Sulfolobus solfataricus]
Length = 332
Score = 363 bits (923), Expect = 1e-99
Identities = 170/328 (51%), Positives = 240/328 (72%), Gaps = 5/328 (1%)
Query: 2 ELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPW 61
+LP+LPT+VIGSYP+P+WL +L + GK+ ED +EA DA +AV ++H +AGVD+P
Sbjct: 3 KLPLLPTTVIGSYPRPKWLRESIRLHKAGKMSNEDLQEAFNDAVIAVFQDHYKAGVDVPT 62
Query: 62 DGEMWRSEMTEHFTAKISGFKFYGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVREN 121
DGE+ R EM E F +I GFKFYGPVRVWG AY+ K + V K+EY+EP++++EF + +
Sbjct: 63 DGEVRRDEMVEFFAERIKGFKFYGPVRVWGTAYYRKPSVVSKIEYREPMLVDEFTFAKSV 122
Query: 122 TTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDEPA 181
+ + +K+ ITGPYTIAEWS+NEYY +K+ V DLAK IN+E+K L E GA IQ+DEPA
Sbjct: 123 SYTDNLKITITGPYTIAEWSYNEYYRNKKDLVFDLAKAINQEIKNLVEAGAKIIQIDEPA 182
Query: 182 MLNHPDEVPLAVEAINRAVKGIKIKVGLHVCYSNYYLLADYFDDIRVTQ--FALEFANRQ 239
+ ++V VEA+N AVKG+ K+ +H+CY +Y +A YF++I+V Q FAL+ N
Sbjct: 183 LHTRKEDVSWGVEAVNEAVKGVNAKLVMHICYGDYSFVAPYFNEIKVDQINFALKIYN-- 240
Query: 240 FRDMDFLKKLS-GKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPEWLYINPDCGLKL 298
++ ++ LKK KELG GVVDVHN ++ET +E+ I+K Y PE ++INPDCGLKL
Sbjct: 241 YKPLELLKKYGFDKELGAGVVDVHNRKVETSEEVANDIRKILEYFPPEKVWINPDCGLKL 300
Query: 299 LDRRIAYQKLVNMVKAVRIVRKELEKEG 326
L R+IAYQKLV+MV+ ++VR+EL+++G
Sbjct: 301 LSRKIAYQKLVSMVEGTKVVREELKRKG 328
>pir||B72509 probable 5-methltetrahydropteroyltriglutamate--homocysteinemethyltr
ansferase APE2048 - Aeropyrum pernix (strain K1)
>gi|5105746|dbj|BAA81058.1| (AP000063) 332aa long
hypothetical
5-methltetrahydropteroyltriglutamate--
homocysteinemethyltr ansferase [Aeropyrum pernix]
Length = 332
Score = 230 bits (581), Expect = 2e-59
Identities = 123/320 (38%), Positives = 188/320 (58%), Gaps = 5/320 (1%)
Query: 7 PTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPWDGEMW 66
PT+V+GSYPK K R G+I EE+F +++ V+ ++ AGVDI DGE
Sbjct: 12 PTTVVGSYPKIGPASEAIKKRRAGEISEEEFHRIARESIRMVVEDYIWAGVDIISDGEQT 71
Query: 67 RSEMTEHFTAKISGFKFYGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVRENTTREI 126
R +M +F ++ G++ VRV+ N YF K ++ + P+++E++ + R +
Sbjct: 72 REDMVVYFAERLEGYREGDWVRVFDNVYFRKPIVAGRVRWVRPMIIEDWEYARSISAGRP 131
Query: 127 VKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDEPAMLNHP 186
VK ITGPYT+ EWSF+ +Y D+ + DLA+ I +E+ GA YIQ+DEPA+ P
Sbjct: 132 VKFIITGPYTMLEWSFDLHYGDRRELIFDLARAIRREIDEALARGAEYIQVDEPALSTRP 191
Query: 187 --DEVPLAVEAINRAVKGIKIKVGLHVCYSNYYLLADYFDDIRVTQFALEFANRQFRDMD 244
+E L EA++ KG+ K +H+CY + Y D V QF LE N FR +
Sbjct: 192 FKEEAELLKEALDIIFKGVGAKRIVHICYGELERILPYILDYPVDQFDLEMKNSNFRLLP 251
Query: 245 FLKKLS-GKELGFGVVDVHNPRIETVDEIVRAIKK--AFNYLEPEWLYINPDCGLKLLDR 301
+LK+ KE+G+GV+DVH+ ++E+V E+ AI + + + PE +Y++PDCGLK L R
Sbjct: 252 YLKEYGYDKEIGYGVIDVHSFQVESVGEVKEAIDRLMKMDIVGPEKVYVDPDCGLKRLPR 311
Query: 302 RIAYQKLVNMVKAVRIVRKE 321
+A KL NMV+A R+ R+E
Sbjct: 312 DVARAKLKNMVEAARLAREE 331
>emb|CAC12106.1| (AL445066) methionine synthase (cobalamin-independent) related
protein [Thermoplasma acidophilum]
Length = 343
Score = 223 bits (563), Expect = 2e-57
Identities = 134/345 (38%), Positives = 201/345 (57%), Gaps = 33/345 (9%)
Query: 6 LPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVD-IPWDGE 64
L T IGS+ KP +L + + ++ + P+ F E + A+ L ER+G+D I GE
Sbjct: 4 LITQEIGSFRKPDYLAKEF--HKIERTPK--FTELAERATRETLEVFERSGLDNIGIGGE 59
Query: 65 MWRSEMTEHFTAKISGFKFYGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVRENTTR 124
M+R EM EH +I G FYG VR + N Y+ K +A+D+LE +EP ++E +V TT+
Sbjct: 60 MFRWEMYEHPAERIKGIIFYGMVRSFDNRYYRKGSAIDRLERREPFHVDEVKFVA-GTTK 118
Query: 125 EIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEE-------HGATYIQL 177
+ +KVPITGPYT+ EWSFN+YY +E M+ A+IIN+ELK + IQ+
Sbjct: 119 KPLKVPITGPYTMMEWSFNDYYDSREDLAMEFARIINEELKDIASVWKQVSGGRRLEIQI 178
Query: 178 DEPAMLNHPDEVPLAVEAINRAVKGIKIKVGLHVCY-SNYYLLADYFDDIRVTQFALEFA 236
DEPA HPDE+ + V++INR+V+G+ ++ +HVCY S+Y LL D D+++ + LE++
Sbjct: 179 DEPATTTHPDEMDIVVDSINRSVQGVDGEISMHVCYSSDYRLLYDRIPDLKIDGYNLEYS 238
Query: 237 NRQ-------------FRDMDFLKKLS-----GKELGFGVVDVHNPRIETVDEIVRAIKK 278
NR F+D+ + +++ K +G GV DVH +E V+ I I
Sbjct: 239 NRDTLERGLTDDKRVGFQDLKYFAQINESLQRKKFIGIGVTDVHIDYVEPVELIEDRINY 298
Query: 279 AFNYL-EPEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKEL 322
A + +P+ + INPDCGL+ R I QKL NMV A + K+L
Sbjct: 299 ALKIIGDPDLVRINPDCGLRTRSREIGEQKLRNMVMARNNILKQL 343
>dbj|BAB04157.1| (AP001508) homosystein methyl transferase [Bacillus halodurans]
Length = 756
Score = 154 bits (385), Expect = 1e-36
Identities = 98/336 (29%), Positives = 167/336 (49%), Gaps = 16/336 (4%)
Query: 2 ELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPW 61
+LP+LPT+ IGS+P+ + + R L G++ +++ +K + E G+D+
Sbjct: 420 QLPLLPTTTIGSFPQTKDVRRTRSLWRKGELSTVEYERTMKSYIEKWINIQEELGLDVLV 479
Query: 62 DGEMWRSEMTEHFTAKISGFKFY--GPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVR 119
GE R++M E F K+ GF F G V+ +G+ + + EP+ + E ++ +
Sbjct: 480 HGEFERNDMVEFFGEKLDGFAFTANGWVQSYGSRCVKPPIIYGNVSFTEPMTVAETVYAQ 539
Query: 120 ENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDE 179
T + VK +TGP TI WSF +A+ + E+ LEE G IQ+DE
Sbjct: 540 SLTDKP-VKGMLTGPVTILNWSFVRDDLPLTVIAHQIAEALTHEVTALEEAGIEMIQIDE 598
Query: 180 PAML--------NHPDEVPLAVEAINRAVKGIKI--KVGLHVCYSNYYLLADYFDDIRVT 229
PA+ + + + AV A + +K ++ H+CYS ++ + + DD+
Sbjct: 599 PAIREGLPLKAEDQQEYLDWAVSAFRASCAHVKATTQIHTHMCYSEFHEMIEAIDDLDAD 658
Query: 230 QFALEFANRQFRDM--DFLKKLSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPEW 287
++E +R +M F K K +G GV D+H+PR+ + +E++ I++A L
Sbjct: 659 VISIE-TSRSHGEMISAFEKTTYEKGIGLGVYDIHSPRVPSEEEMLNVIRRALTVLPASL 717
Query: 288 LYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKELE 323
++NPDCGLK + L NMV A R R+EL+
Sbjct: 718 FWVNPDCGLKTRAEKETVAALKNMVAAARAAREELK 753
>sp|O67606|METE_AQUAE 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE
METHYLTRANSFERASE (METHIONINE SYNTHASE, VITAMIN-B12
INDEPENDENT ISOZYME) (COBALAMIN-INDEPENDENT METHIONINE
SYNTHASE) >gi|7433426|pir||D70447
tetrahydropteroyltriglutamate methyltransferase -
Aquifex aeolicus >gi|2984022|gb|AAC07565.1| (AE000752)
tetrahydropteroyltriglutamate methyltransferase [Aquifex
aeolicus]
Length = 761
Score = 153 bits (384), Expect = 2e-36
Identities = 97/335 (28%), Positives = 164/335 (48%), Gaps = 14/335 (4%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
++LP+ PT+ IGS+P+ + +M GKI EE++K +K + E G+D+
Sbjct: 424 LKLPLFPTTTIGSFPQTEDVRKMRTAYRTGKISEEEYKNFIKQKIKYAIEVQEDIGLDVL 483
Query: 61 WDGEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWV 118
GE RS+M E+F K+ G +G V +G+ + + P+ LEE +
Sbjct: 484 VHGEFERSDMVEYFAIKLDGIATTKHGWVLSYGSRVYRPPIIYGDVSRPAPMTLEEITYA 543
Query: 119 RENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLD 178
++ T + VK +TGP TI WS+ K +A + E+K LE G IQ+D
Sbjct: 544 -QSLTNKPVKGMLTGPVTILNWSYYREDVPKREIAYQIALALLDEVKDLENAGIKVIQID 602
Query: 179 EPAM--------LNHPDEVPLAVEAINRAVKG-IKIKVGLHVCYSNYYLLADYFDDIRVT 229
EPA + P+ AV+A A + + ++ H+CYS + + +Y +
Sbjct: 603 EPAFREGAPLKKRDWPEYFDWAVKAFRLASRAKPETQIHTHMCYSEFNEIIEYIYQMDFD 662
Query: 230 QFALEFANRQFRDMDFLKKLSG--KELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPEW 287
++E + + ++ +K G +++G GV D+H+P I T +E+ + +A L E
Sbjct: 663 VISIEASRSKGEIIEVFEKFKGWDRQIGIGVYDIHSPAIPTKEEMKAIMLRAMKVLPKEL 722
Query: 288 LYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKEL 322
L++NPDCGLK L NMV + +R+E+
Sbjct: 723 LWVNPDCGLKTRRWEEVIPALRNMVDMAKELREEV 757
>sp|Q9X112|METE_THEMA 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE
METHYLTRANSFERASE (METHIONINE SYNTHASE, VITAMIN-B12
INDEPENDENT ISOZYME) (COBALAMIN-INDEPENDENT METHIONINE
SYNTHASE) >gi|7433429|pir||E72271
5-methyltetrahydropteroyltriglutamate-- homocysteine
methyltransferase - Thermotoga maritima (strain MSB8)
>gi|4981844|gb|AAD36360.1|AE001784_2 (AE001784)
5-methyltetrahydropteroyltriglutamate--homocysteine
methyltransferase [Thermotoga maritima]
Length = 734
Score = 152 bits (381), Expect = 4e-36
Identities = 94/337 (27%), Positives = 170/337 (49%), Gaps = 16/337 (4%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
+ LP+ PT+ IGS+P+ + +M G+I +E+++ +K+ + E G+D+
Sbjct: 399 LNLPLFPTTTIGSFPQTPEVRKMRSKYRKGEISKEEYEAFIKEQIKKAIELQEEIGLDVL 458
Query: 61 WDGEMWRSEMTEHFTAKISGFKFY--GPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWV 118
GE R++M E F K++G G V +G+ + + EP+ L+E +
Sbjct: 459 VHGEFERTDMVEFFAEKLNGIATTQNGWVLSYGSRCYRPPIIYGTVTRPEPMTLKEITYA 518
Query: 119 RENTTREIVKVPITGPYTIAEWSF-NEYYPDKESFVMDLAKIINKELKTLEEHGATYIQL 177
++ T + VK +TGP TI WS+ E P++E +A IN+E+K LEE G +Q+
Sbjct: 519 -QSLTEKPVKGMLTGPVTIMSWSYYREDIPERE-IAYQIALAINEEVKDLEEAGIKIVQI 576
Query: 178 DEPAMLNH--------PDEVPLAVEAINRAVKGI-KIKVGLHVCYSNYYLLADYFDDIRV 228
DEPA P+ A+ A N A + ++ H+CYS++ + +Y +
Sbjct: 577 DEPAFREKAPIKKSKWPEYFEWAINAFNLAANARPETQIHAHMCYSDFNEIIEYIHQLEF 636
Query: 229 TQFALEFANRQFRDMDFLKKLSG--KELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPE 286
++E + + + + G K++G GV D+H+P + +++E+ +++ L E
Sbjct: 637 DVISIEASRSKGEIISAFENFKGWIKQIGVGVWDIHSPAVPSINEMREIVERVLRVLPKE 696
Query: 287 WLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKELE 323
++INPDCGLK + L NMV + +R++ E
Sbjct: 697 LIWINPDCGLKTRNWDEVIPSLRNMVALAKEMREKFE 733
>sp|P80877|METE_BACSU 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE
METHYLTRANSFERASE (METHIONINE SYNTHASE, VITAMIN-B12
INDEPENDENT ISOZYME) (COBALAMIN-INDEPENDENT METHIONINE
SYNTHASE) (SUPEROXIDE-INDUCIBLE PROTEIN 9) (SOI9)
>gi|7433425|pir||C69657 cobalamin-independent methionine
synthase metC - Bacillus subtilis
>gi|2632038|emb|CAA05597.1| (AJ002571) MetC [Bacillus
subtilis] >gi|2633672|emb|CAB13175.1| (Z99110)
cobalamin-independent methionine synthase [Bacillus
subtilis]
Length = 762
Score = 148 bits (371), Expect = 7e-35
Identities = 92/332 (27%), Positives = 163/332 (48%), Gaps = 14/332 (4%)
Query: 3 LPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPWD 62
LP+LPT+ IGS+P+ + R + + +E ++ + + + E +D+
Sbjct: 427 LPLLPTTTIGSFPQSAEVRRARQKWRKAEWSDEQYQNFINAETKRWIDIQEELELDVLVH 486
Query: 63 GEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVRE 120
GE R++M E+F K++GF F Y V+ +G+ +E+ EP+ +++ ++ +
Sbjct: 487 GEFERTDMVEYFGEKLAGFAFTKYAWVQSYGSRCVRPPVIYGDVEFIEPMTVKDTVYAQS 546
Query: 121 NTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDEP 180
T++ VK +TGP TI WSF ++ + + KE+K LE+ G IQ+DEP
Sbjct: 547 LTSKH-VKGMLTGPVTILNWSFPRNDISRKEIAFQIGLALRKEVKALEDAGIQIIQVDEP 605
Query: 181 AML--------NHPDEVPLAVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDIRVTQ 230
A+ + + + A EA +K ++ H+CYSN+ + D +D+
Sbjct: 606 ALREGLPLKTRDWDEYLTWAAEAFRLTTSSVKNETQIHTHMCYSNFEDIVDTINDLDADV 665
Query: 231 FALEFANRQFRDMDFLKKLSG-KELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPEWLY 289
+E + +D+LK K LG GV D+H+PR+ + +E+ I A + +
Sbjct: 666 ITIEHSRSHGGFLDYLKNHPYLKGLGLGVYDIHSPRVPSTEEMYNIIVDALAVCPTDRFW 725
Query: 290 INPDCGLKLLDRRIAYQKLVNMVKAVRIVRKE 321
+NPDCGLK + L NMV+A + R +
Sbjct: 726 VNPDCGLKTRQQEETVAALKNMVEAAKQARAQ 757
>gb|AAF81245.1| (AF263012) 5-methyltetrahydropteroyltriglutamate-homocysteine
methyltransferase-like protein [Streptomyces griseus
subsp. griseus]
Length = 774
Score = 148 bits (369), Expect = 1e-34
Identities = 99/339 (29%), Positives = 161/339 (47%), Gaps = 20/339 (5%)
Query: 3 LPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPWD 62
LP+LPT+ IGS+P+ L G+I E ++E +KD VL E+AG+D+
Sbjct: 438 LPLLPTTTIGSFPQTTELRTARADLRAGRIDEAGYEERIKDEIREVLSFQEKAGLDVLVH 497
Query: 63 GEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVRE 120
GE R++M ++F +++G+ +G V+ +G Y + EP+ + + +
Sbjct: 498 GEPERNDMVQYFAEQLTGYLATQHGWVQSYGTRYVRPPVLAGDISRPEPMTVRWTTYAQS 557
Query: 121 NTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDEP 180
T R VK +TGP T+ WSF +A + E+ LE G + IQ+DEP
Sbjct: 558 LTARP-VKGMLTGPVTMLAWSFVRDDQPLGDTARQVALALRDEVNDLEASGTSVIQVDEP 616
Query: 181 AM--------LNHPDEVPLAVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDIRVTQ 230
A+ H + A E+ A G++ ++ H+CY+ + + DD+
Sbjct: 617 ALRETLPLRAAEHAAYLEWATESFRLATGGVRPDTQIHTHMCYAEFGDIVQAIDDLDADV 676
Query: 231 FALEFANRQFRDMDFLKKLSG----KELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPE 286
+LE A M ++L+ +E G GV D+H+PRI + +E ++K +
Sbjct: 677 ISLEAARSH---MQVARELAAHGYPREAGPGVYDIHSPRIPSTEEAAALLRKGLEAIPAS 733
Query: 287 WLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKELEKE 325
L++NPDCGLK L N+V A R +R EL E
Sbjct: 734 RLWVNPDCGLKTRGWPETRASLENLVAAAREIRAELPTE 772
>gb|AAF00639.1|AC009540_16 (AC009540) putative methionine synthase [Arabidopsis thaliana]
Length = 765
Score = 141 bits (351), Expect = 1e-32
Identities = 99/338 (29%), Positives = 163/338 (47%), Gaps = 19/338 (5%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
+ LPILPT+ IGS+P+ L R+ + + KI EED+ +A+K+ V+ E +D+
Sbjct: 427 LNLPILPTTTIGSFPQTVELRRVRREYKAKKISEEDYVKAIKEEIKKVVDIQEDLDIDVL 486
Query: 61 WDGEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLW- 117
GE R++M E+F ++SGF F G V+ +G+ + +P+ + W
Sbjct: 487 VHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPVIYGDVSRPKPMTV---FWS 543
Query: 118 -VRENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQ 176
++ T+ +K +TGP TI WSF + +A I E++ LE+ G IQ
Sbjct: 544 STAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKGGIGVIQ 603
Query: 177 LDEPAM--------LNHPDEVPLAVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDI 226
+DE A+ H + AV + G++ ++ H+CYSN+ + D+
Sbjct: 604 IDEAALREGLPLRKAEHSFYLDWAVHSFRITNCGVQDSTQIHTHMCYSNFNDIIHSIIDM 663
Query: 227 RVTQFALEFANRQFRDMDFLKK--LSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLE 284
+E + + + ++ G +G GV D+H+PRI + DEI I K LE
Sbjct: 664 DADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTDEIADRINKMLAVLE 723
Query: 285 PEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKEL 322
L++NPDCGLK L MV A +++R +L
Sbjct: 724 QNILWVNPDCGLKTRKYTEVKPALKAMVDAAKLIRSQL 761
>pir||C81326 5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase (EC 2.1.1.14) Cj1201 [imported] -
Campylobacter jejuni (strain NCTC 11168)
>gi|6968634|emb|CAB73455.1| (AL139077)
5-methyltetrahydropteroyltriglutamate--homocystei
methyltransferase [Campylobacter jejuni]
Length = 754
Score = 141 bits (351), Expect = 1e-32
Identities = 98/338 (28%), Positives = 163/338 (47%), Gaps = 19/338 (5%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
++LP+LPT+ IGS+P+ + + KI +++ E + ++ E G+D+
Sbjct: 420 LKLPLLPTTTIGSFPQSADVRSNRLAFKQEKISAQNYTEFNQQKIKECIQIQEEIGLDVL 479
Query: 61 WDGEMWRSEMTEHFTAKISGFKFY--GPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWV 118
GE R++M E+F + GF F G V+ +G + +P+ L W
Sbjct: 480 VHGEFERNDMVEYFGENLKGFLFTQNGWVQSYGTRCVKPPVIWGDVSRTKPITLA---WS 536
Query: 119 R--ENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQ 176
+ ++ +++IVK +TGP TI WSF + +A I E+ LE G IQ
Sbjct: 537 KFAQSLSQKIVKGMLTGPVTILNWSFPREDISLKESTEQIALAIRDEVLDLENAGIKIIQ 596
Query: 177 LDEPAMLN---------HPDEVPLAVEAINRAVKGIKIKVGLH--VCYSNYYLLADYFDD 225
+DE A+ H + + A+ A N G+K K +H +CYS + + D
Sbjct: 597 IDEAALREKLPLRKSDWHSEYLDWAIPAFNLVHSGVKAKTQIHTHMCYSEFSDILKEIDA 656
Query: 226 IRVTQFALEFANRQFRDMDFLKKLSGK-ELGFGVVDVHNPRIETVDEIVRAIKKAFNYLE 284
+ + E + +D LK + K E+G GV D+H+PR+ +V+E+ I+K N L
Sbjct: 657 MDADVISFEASRSNLSLLDTLKAIRFKTEVGPGVYDIHSPRVPSVEELSLTIEKILNKLP 716
Query: 285 PEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKEL 322
E ++INPDCGLK L N+V A + +R++L
Sbjct: 717 KEQIWINPDCGLKTRAYEEVITSLKNLVTATQKIREQL 754
>sp|P93263|METE_MESCR 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE
METHYLTRANSFERASE (VITAMIN-B12-INDEPENDENT METHIONINE
SYNTHASE ISOZYME) (COBALAMIN-INDEPENDENT METHIONINE
SYNTHASE ISOZYME) >gi|7433427|pir||T12575
5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase (EC 2.1.1.14) - common ice plant
>gi|1814403|gb|AAB41896.1| (U84889) methionine synthase
[Mesembryanthemum crystallinum]
Length = 765
Score = 140 bits (350), Expect = 2e-32
Identities = 98/338 (28%), Positives = 164/338 (47%), Gaps = 19/338 (5%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
+ LPILPT+ IGS+P+ L R+ + + KI EE++ +A+K+ V++ E +D+
Sbjct: 427 LNLPILPTTTIGSFPQTVELRRVRREYKAKKISEEEYVKAIKEEISKVVKLQEELDIDVL 486
Query: 61 WDGEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLW- 117
GE R++M E+F ++SGF F G V+ +G+ + P+ + W
Sbjct: 487 VHGEPERNDMVEYFGEQLSGFAFSANGWVQSYGSRCVKPPIIYGDVSRPNPMTV---FWS 543
Query: 118 -VRENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQ 176
+ ++ T +K +TGP TI WSF + +A I E++ LE+ G IQ
Sbjct: 544 SMAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKNEVEDLEKAGINVIQ 603
Query: 177 LDEPAM--------LNHPDEVPLAVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDI 226
+DE A+ H + AV + G++ ++ H+CYSN+ + D+
Sbjct: 604 IDEAALREGLPLRKSEHDFYLKWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIIDM 663
Query: 227 RVTQFALEFANRQFRDMDFLKK--LSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLE 284
+E + + + ++ G +G GV D+H+PRI +E+ I+K LE
Sbjct: 664 DADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPPTEELADRIRKMLAVLE 723
Query: 285 PEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKEL 322
L++NPDCGLK L NMV A + +R+EL
Sbjct: 724 SNVLWVNPDCGLKTRKYGEVNPALSNMVAAAKQLRQEL 761
>pir||D83404 5-methyltetrahydropteroyltriglutamate- homocysteine
S-methyltransferase PA1927 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9947922|gb|AAG05315.1|AE004619_2 (AE004619)
5-methyltetrahydropteroyltriglutamate-homocysteine
S-methyltransferase [Pseudomonas aeruginosa]
Length = 766
Score = 140 bits (350), Expect = 2e-32
Identities = 98/342 (28%), Positives = 164/342 (47%), Gaps = 24/342 (7%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
++LP PT+ IGS+P+ + + + GK+ E ++ EA+ ++ E+ G+D+
Sbjct: 430 LDLPAFPTTTIGSFPQTSSIRLARQSFKQGKLSEAEYIEAMHSEIRHAVQIQEQLGLDVL 489
Query: 61 WDGEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWV 118
GE R++M E+F ++ G+ F +G V+ +G+ A L + +E W+
Sbjct: 490 VHGEAERNDMVEYFAEQLDGYVFTRFGWVQSYGSRCVKPAVIYGDLSRPRAMTVE---WI 546
Query: 119 R--ENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQ 176
R ++ T +++K +TGP T+ WSF +E LA I E+ LE G +Q
Sbjct: 547 RYAQSLTDKVMKGMLTGPVTMLMWSFPREDVSREVQARQLALAIRDEVVDLEAAGIRIVQ 606
Query: 177 LDEPAMLNH--------PDEVPLAVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDI 226
+DE A P + A EA G++ ++ H+CYS + D + I
Sbjct: 607 IDEAAFREGLPLRRNAWPHYLEWATEAFRLCASGVRDETQIHTHMCYSEFN---DVIESI 663
Query: 227 RVTQFALEFANRQFRDMDFLKKLSG----KELGFGVVDVHNPRIETVDEIVRAIKKAFNY 282
+ DM+ L+ E+G GV D+H+PR+ + +EIV ++KA
Sbjct: 664 AAMDADVITIETSRSDMELLEAFEQFDYPNEIGPGVYDIHSPRVPSREEIVALLRKAARR 723
Query: 283 LEPEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKELEK 324
+ E L++NPDCGLK LVNMV A R +R +L +
Sbjct: 724 IPAERLWVNPDCGLKTRAWPETEAALVNMVAAARELRGDLAR 765
>sp|P57142|METE_BUCAI 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE
METHYLTRANSFERASE (METHIONINE SYNTHASE, VITAMIN-B12
INDEPENDENT ISOZYME) (COBALAMIN-INDEPENDENT METHIONINE
SYNTHASE) >gi|10038722|dbj|BAB12757.1| (AP001118)
5-methyltetrahydropteroyltriglutamate-homocystei ne
S-methyltransferase [Buchnera sp. APS]
Length = 758
Score = 140 bits (349), Expect = 2e-32
Identities = 92/338 (27%), Positives = 167/338 (49%), Gaps = 24/338 (7%)
Query: 4 PILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPWDG 63
PILPT+ IGS+P+ + ++ + +LG + EE++ + +K V++ E +D+ G
Sbjct: 422 PILPTTTIGSFPQTISIRKLRRDFKLGLVTEEEYTKIIKKNIKKVIKIQEELDIDVLVHG 481
Query: 64 EMWRSEMTEHFTAKISGFKFY--GPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVR-- 119
E R++M E+F + GF F G V+ +G+ + + +P+ +E W +
Sbjct: 482 EAERNDMVEYFGEHLDGFAFTDNGWVQSYGSRCVKPPIIIGDISRPKPMTIE---WSKYA 538
Query: 120 ENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDE 179
++ T++ VK +TGP TI WSF + +A + E+ LE+ IQ+DE
Sbjct: 539 QSLTKKPVKGMLTGPVTILLWSFPREDVSLKKIATQIALALYDEVLDLEKEKIEIIQIDE 598
Query: 180 PAMLNHPDEVPL-----------AVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDI 226
PA+ + +PL AV+ + G+K ++ H+CY + + D +
Sbjct: 599 PALR---EGLPLRKSSWHEYLSWAVDVFRLSASGVKNTTQIHTHMCYCEFNDIMDSIALL 655
Query: 227 RVTQFALEFANRQFRDMDFLKKLS-GKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEP 285
+E A ++ KK E+G G D+H+ I +V I+ + KA Y+
Sbjct: 656 DADVITIEAARSDMELLESFKKFKYPNEVGPGAYDIHSSNIPSVQSIISLLNKAMKYIPL 715
Query: 286 EWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKELE 323
+ +++NPDCGLK + L NMV+A +I+R++++
Sbjct: 716 KRIWVNPDCGLKTRNWNETILSLKNMVEATKILREKMK 753
>sp|Q42699|METE_CATRO 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE
METHYLTRANSFERASE (VITAMIN-B12-INDEPENDENT METHIONINE
SYNTHASE ISOZYME) (COBALAMIN-INDEPENDENT METHIONINE
SYNTHASE ISOZYME) >gi|1362086|pir||S57636
5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase (EC 2.1.1.14) - Madagascar
periwinkle >gi|2129919|pir||S65957
5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase (EC 2.1.1.14) - Madagascar
periwinkle >gi|886471|emb|CAA58474.1| (X83499)
methionine synthase [Catharanthus roseus]
Length = 765
Score = 138 bits (345), Expect = 7e-32
Identities = 97/341 (28%), Positives = 166/341 (48%), Gaps = 25/341 (7%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
+ LP+LPT+ IGS+P+ L R+ + + KI E+D+ +A+K+ V++ E +D+
Sbjct: 427 LNLPVLPTTTIGSFPQTLELRRVRREYKAKKISEDDYVKAIKEEISKVVKLQEELDIDVL 486
Query: 61 WDGEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLW- 117
GE R++M E+F ++SGF F G V+ +G+ + P+ + W
Sbjct: 487 VHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPNPMTV---FWS 543
Query: 118 -VRENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQ 176
++ T+ +K +TGP TI WSF + +A I E++ LE+ G IQ
Sbjct: 544 QTAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGINVIQ 603
Query: 177 LDEPAMLNHPDEVPLAVEA----INRAVKGIKI---------KVGLHVCYSNYYLLADYF 223
+DE A+ + +PL ++ AV +I ++ H+CYSN+ +
Sbjct: 604 IDEAAL---REGLPLRKAEHAFYLDWAVHSFRITNLPLQDTTQIHTHMCYSNFNDIIHSI 660
Query: 224 DDIRVTQFALEFANRQFRDMDFLKK--LSGKELGFGVVDVHNPRIETVDEIVRAIKKAFN 281
D+ +E + + + ++ G +G GV D+H+PRI + +EI I K
Sbjct: 661 IDMDADVMTIENSRSSEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA 720
Query: 282 YLEPEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKEL 322
L+ L++NPDCGLK L NMV A +++R +L
Sbjct: 721 VLDTNILWVNPDCGLKTRKYAEVKPALENMVSAAKLIRTQL 761
>emb|CAA89019.1| (Z49150) cobalamine-independent methionine synthase [Solenostemon
scutellarioides]
Length = 784
Score = 138 bits (344), Expect = 1e-31
Identities = 97/338 (28%), Positives = 166/338 (48%), Gaps = 19/338 (5%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
+ LPILPT+ IGS+P+ L R+ + + +I EE++ +A+K+ V++ E +D+
Sbjct: 446 LNLPILPTTTIGSFPQTVELRRVRREFKPTRISEEEYVKAIKEEINKVVKLQEELDIDVL 505
Query: 61 WDGEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLW- 117
GE R++M E+F ++SGF F G V+ +G+ + +P+ + W
Sbjct: 506 VHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKPMTV---FWS 562
Query: 118 -VRENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQ 176
++ T+ +K +TGP TI WSF + +A I E++ LE+ G T IQ
Sbjct: 563 TAAQSMTQRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGITVIQ 622
Query: 177 LDEPAM--------LNHPDEVPLAVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDI 226
+DE A+ H + AV + G++ ++ H+CYSN+ + ++
Sbjct: 623 IDEAALREGLPLRKSEHAFYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIINM 682
Query: 227 RVTQFALEFANRQFRDMDFLKK--LSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLE 284
+E + + + ++ G +G GV D+H+PRI + +EI I K LE
Sbjct: 683 DADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLE 742
Query: 285 PEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKEL 322
L++NPDCGLK L NMV A +++R +L
Sbjct: 743 TNILWVNPDCGLKTRKYAEVKPALENMVAAAKLLRTQL 780
>sp|Q42662|METE_SOLSC 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE
METHYLTRANSFERASE (VITAMIN-B12-INDEPENDENT METHIONINE
SYNTHASE ISOZYME) (COBALAMIN-INDEPENDENT METHIONINE
SYNTHASE ISOZYME)
Length = 764
Score = 138 bits (344), Expect = 1e-31
Identities = 97/338 (28%), Positives = 166/338 (48%), Gaps = 19/338 (5%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
+ LPILPT+ IGS+P+ L R+ + + +I EE++ +A+K+ V++ E +D+
Sbjct: 426 LNLPILPTTTIGSFPQTVELRRVRREFKPTRISEEEYVKAIKEEINKVVKLQEELDIDVL 485
Query: 61 WDGEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLW- 117
GE R++M E+F ++SGF F G V+ +G+ + +P+ + W
Sbjct: 486 VHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKPMTV---FWS 542
Query: 118 -VRENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQ 176
++ T+ +K +TGP TI WSF + +A I E++ LE+ G T IQ
Sbjct: 543 TAAQSMTQRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGITVIQ 602
Query: 177 LDEPAM--------LNHPDEVPLAVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDI 226
+DE A+ H + AV + G++ ++ H+CYSN+ + ++
Sbjct: 603 IDEAALREGLPLRKSEHAFYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIINM 662
Query: 227 RVTQFALEFANRQFRDMDFLKK--LSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLE 284
+E + + + ++ G +G GV D+H+PRI + +EI I K LE
Sbjct: 663 DADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLE 722
Query: 285 PEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKEL 322
L++NPDCGLK L NMV A +++R +L
Sbjct: 723 TNILWVNPDCGLKTRKYAEVKPALENMVAAAKLLRTQL 760
>sp|O50008|METE_ARATH 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE
METHYLTRANSFERASE (VITAMIN-B12-INDEPENDENT METHIONINE
SYNTHASE ISOZYME) (COBALAMIN-INDEPENDENT METHIONINE
SYNTHASE ISOZYME) >gi|2738248|gb|AAC50037.1| (U97200)
cobalamin-independent methionine synthase [Arabidopsis
thaliana] >gi|10177894|dbj|BAB11226.1| (AB011480)
cobalamin-independent methionine synthase [Arabidopsis
thaliana]
Length = 765
Score = 137 bits (342), Expect = 2e-31
Identities = 96/338 (28%), Positives = 163/338 (47%), Gaps = 19/338 (5%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
+ LPILPT+ IGS+P+ L R+ + + K+ EED+ +A+K+ V+ E +D+
Sbjct: 427 LNLPILPTTTIGSFPQTVELRRVRREYKAKKVSEEDYVKAIKEEIKKVVDLQEELDIDVL 486
Query: 61 WDGEMWRSEMTEHFTAKISGFKFYGPVRVWGNAYFNKAAAVDKL--EYKEPLVLEEFLW- 117
GE R++M E+F ++SGF F W +Y ++ + + P + F W
Sbjct: 487 VHGEPERNDMVEYFGEQLSGFAF--TANGWVQSYGSRCVKPPVIYGDVSRPKAMTVF-WS 543
Query: 118 -VRENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQ 176
+ ++ T +K +TGP TI WSF + +A I E++ LE+ G IQ
Sbjct: 544 AMAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKGGIGVIQ 603
Query: 177 LDEPAM--------LNHPDEVPLAVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDI 226
+DE A+ H + AV + G++ ++ H+CYS++ + D+
Sbjct: 604 IDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDSTQIHTHMCYSHFNDIIHSIIDM 663
Query: 227 RVTQFALEFANRQFRDMDFLKK--LSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLE 284
+E + + + ++ G +G GV D+H+PRI + +EI + K LE
Sbjct: 664 DADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSSEEIADRVNKMLAVLE 723
Query: 285 PEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKEL 322
L++NPDCGLK L NMV A +++R +L
Sbjct: 724 QNILWVNPDCGLKTRKYTEVKPALKNMVDAAKLIRSQL 761
>gb|AAF74983.1|AF082893_1 (AF082893) methionine synthase [Solanum tuberosum]
Length = 765
Score = 137 bits (342), Expect = 2e-31
Identities = 98/336 (29%), Positives = 162/336 (48%), Gaps = 15/336 (4%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
+ LPILPT+ IGS+P+ L R+ + + KI EE++ +A+ + V+ E +D+
Sbjct: 427 LNLPILPTTTIGSFPQTVELRRVRREYKAKKISEEEYVKAITEEIKKVVDLQEELDIDVL 486
Query: 61 WDGEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWV 118
GE R++M E+F ++SGF F G V+ +G+ + +P+ +
Sbjct: 487 VHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWSSKA 546
Query: 119 RENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLD 178
+E T R +K +TGP TI WSF + +A I E++ LE+ G T IQ+D
Sbjct: 547 QEMTKRP-MKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGITVIQID 605
Query: 179 EPAM--------LNHPDEVPLAVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDIRV 228
E A+ H + AV + GI+ ++ H+CYSN+ + D+
Sbjct: 606 EAALREGLPLRKAEHAFYLNWAVHSFRITNVGIEDTTQIHTHMCYSNFNDIIHSIIDMDA 665
Query: 229 TQFALEFANRQFRDMDFLKK--LSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPE 286
+E + + + ++ G +G GV D+H+PRI + +EI + K L+
Sbjct: 666 DVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRVNKMLAVLDTN 725
Query: 287 WLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKEL 322
L++NPDCGLK L NMV A + +R +L
Sbjct: 726 ILWVNPDCGLKTRKYTEVKPALQNMVSAAKTIRTQL 761
>pir||E82167 5-methyltetrahydropteroyltriglutamate-- homocysteine
methyltransferase VC1704 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9656222|gb|AAF94854.1|
(AE004248)
5-methyltetrahydropteroyltriglutamate--homocysteine
methyltransferase [Vibrio cholerae]
Length = 761
Score = 136 bits (340), Expect = 3e-31
Identities = 91/335 (27%), Positives = 164/335 (48%), Gaps = 20/335 (5%)
Query: 3 LPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPWD 62
LP LPT+ IGS+P+ + GK+ E+++ +A+K ++ E G+D+
Sbjct: 428 LPFLPTTTIGSFPQTGEIRTERSAYRQGKLSEQEYVQALKGHIADAVKRQEALGLDVLVH 487
Query: 63 GEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVRE 120
GE R++M E+F ++GF+ +G V+ +G+ A V +E ++P+ +E + +
Sbjct: 488 GEAERNDMVEYFAENLAGFQTTQFGWVQSYGSRCVKPAIVVADIEREKPITVEWSTYAQS 547
Query: 121 NTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDEP 180
T++++ K +TGP TI W+F ++ LA + E+ L++ G IQ+DEP
Sbjct: 548 LTSKQM-KGMLTGPVTILCWTFPREDISRQEIAQQLALALRDEVADLQDAGINIIQIDEP 606
Query: 181 AML--------NHPDEVPLAVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDIRVTQ 230
A+ +H + AV+A + + ++ H+CYS + + + +
Sbjct: 607 AIREGLPLKKRDHQTYLDWAVQAFKISAGSARPETQIHTHMCYSEFNEIIESVAALDADV 666
Query: 231 FALEFANRQFRDMDFLKKLSG----KELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPE 286
+E + +M+ LK E+G GV D+H+P I I ++KA + +
Sbjct: 667 ITIETSRS---NMELLKAFEEFNYPNEIGPGVYDIHSPNIPAQAWIEDLLRKAAEKIPAQ 723
Query: 287 WLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKE 321
L++NPDCGLK + L NMV A + +R E
Sbjct: 724 RLWVNPDCGLKTRNWPEVEAALTNMVNAAKALRAE 758
>gi|6320936 vitamin B12-(cobalamin)-independent isozyme of methionine synthase
(also called N5-methyltetrahydrofolate homocysteine
methyltransferase or 5-methyltetrahydropteroyl
triglutamate homocysteine methyltransferase); Met6p
[Saccharomyces cerevisiae]
>gi|730018|sp|P05694|METE_YEAST
5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE
METHYLTRANSFERASE (METHIONINE SYNTHASE, VITAMIN-B12
INDEPENDENT ISOZYME) (COBALAMIN-INDEPENDENT METHIONINE
SYNTHASE) (DELTA-P8 PROTEIN) >gi|1071830|pir||S50594
5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase (EC 2.1.1.14) - yeast (Saccharomyces
cerevisiae) >gi|603329|gb|AAB64646.1| (U18839) Met6p:
5-methyltetrahydropteroyl triglutamate--homocysteine
methyltransferase [Saccharomyces cerevisiae]
>gi|976441|gb|AAB60301.1| (U32508)
N5-methyltetrahydrofolate homocysteine methyltransferase
[Saccharomyces cerevisiae]
Length = 767
Score = 136 bits (338), Expect = 5e-31
Identities = 94/337 (27%), Positives = 165/337 (48%), Gaps = 26/337 (7%)
Query: 3 LPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPWD 62
LP+ PT+ IGS+P+ + + G I E++++ + V+R E G+D+
Sbjct: 436 LPLFPTTTIGSFPQTKDIRINRNKFNKGTISAEEYEKFINSEIEKVIRFQEEIGLDVLVH 495
Query: 63 GEMWRSEMTEHFTAKISGFKFY--GPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVRE 120
GE R++M ++F +I+G+ F G V+ +G+ Y V L + + ++E ++ +
Sbjct: 496 GEPERNDMVQYFGEQINGYAFTVNGWVQSYGSRYVRPPIIVGDLSRPKAMSVKESVYAQS 555
Query: 121 NTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDEP 180
T++ VK +TGP T WSF D+++ M LA + E+ LE G IQ+DEP
Sbjct: 556 ITSKP-VKGMLTGPITCLRWSFPRDDVDQKTQAMQLALALRDEVNDLEAAGIKVIQVDEP 614
Query: 181 AMLNHPDEVPL------------AVEAINRAVKGI--KIKVGLHVCYSNYYLLADYFDDI 226
A+ + +PL A EA A G+ K ++ H CYS+ L ++ +
Sbjct: 615 ALR---EGLPLREGTERSAYYTWAAEAFRVATSGVANKTQIHSHFCYSD--LDPNHIKAL 669
Query: 227 RVTQFALEFANRQFRDMDFLKKLSG--KELGFGVVDVHNPRIETVDEIVRAIKKAFNYLE 284
++EF+ + D +++ + +G G+ D+H+PRI + DE + I
Sbjct: 670 DADVVSIEFSKKD--DANYIAEFKNYPNHIGLGLFDIHSPRIPSKDEFIAKISTILKSYP 727
Query: 285 PEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKE 321
E ++NPDCGLK L +MV+A + R++
Sbjct: 728 AEKFWVNPDCGLKTRGWEETRLSLTHMVEAAKYFREQ 764
>gb|AAA65711.1| (U15099) methionine synthase [Saccharomyces cerevisiae]
Length = 767
Score = 136 bits (338), Expect = 5e-31
Identities = 94/337 (27%), Positives = 165/337 (48%), Gaps = 26/337 (7%)
Query: 3 LPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPWD 62
LP+ PT+ IGS+P+ + + G I E++++ + V+R E G+D+
Sbjct: 436 LPLFPTTTIGSFPQTKDIRINRNKFNKGTISAEEYEKFINSEIEKVIRFQEEIGLDVLVH 495
Query: 63 GEMWRSEMTEHFTAKISGFKFY--GPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVRE 120
GE R++M ++F +I+G+ F G V+ +G+ Y V L + + ++E ++ +
Sbjct: 496 GEPERNDMVQYFGEQINGYAFTVNGWVQSYGSRYVRPPIIVGDLSRPKAMSVKESVYAQS 555
Query: 121 NTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDEP 180
T++ VK +TGP T WSF D+++ M LA + E+ LE G IQ+DEP
Sbjct: 556 ITSKP-VKGMLTGPITCLRWSFPRDDVDQKTQAMQLALALRDEVNDLEAAGIKVIQVDEP 614
Query: 181 AMLNHPDEVPL------------AVEAINRAVKGI--KIKVGLHVCYSNYYLLADYFDDI 226
A+ + +PL A EA A G+ K ++ H CYS+ L ++ +
Sbjct: 615 ALR---EGLPLREGTERSAYYTWAAEAFRVATSGVANKTQIHSHFCYSD--LDPNHIKAL 669
Query: 227 RVTQFALEFANRQFRDMDFLKKLSG--KELGFGVVDVHNPRIETVDEIVRAIKKAFNYLE 284
++EF+ + D +++ + +G G+ D+H+PRI + DE + I
Sbjct: 670 DADVVSIEFSKKD--DANYIAEFKNYPNHIGLGLFDIHSPRIPSKDEFIAKISTILKSYP 727
Query: 285 PEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKE 321
E ++NPDCGLK L +MV+A + R++
Sbjct: 728 AEKFWVNPDCGLKTRGWEETRLSLTHMVEAAKYFREQ 764
>pir||F82578 5-methyltetrahydropteroyltriglutamate-- homocysteine
methyltransferase XF2272 [imported] - Xylella fastidiosa
(strain 9a5c) >gi|9107431|gb|AAF85071.1|AE004039_6
(AE004039)
5-methyltetrahydropteroyltriglutamate--homocysteine
methyltransferase [Xylella fastidiosa]
Length = 758
Score = 134 bits (334), Expect = 1e-30
Identities = 92/334 (27%), Positives = 162/334 (47%), Gaps = 14/334 (4%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
+ LP PT+ IGS+P+ + + GK+ E D++ +K + +R E G+D+
Sbjct: 426 LNLPAYPTTTIGSFPQTLEVREARAQFKSGKLSESDYETFLKAETERCIRTQEEIGLDVL 485
Query: 61 WDGEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWV 118
GE R++M E+F ++ GF F G V+ +G+ + P+ + +
Sbjct: 486 VHGEFERNDMVEYFGEQLDGFIFTKLGWVQSYGSRCVKPPIIYGDVVRPAPMTVTWSAYA 545
Query: 119 RENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLD 178
+ T + + K +TGP T+ +WSF ++ +A + E++ LE+ G IQ+D
Sbjct: 546 QSLTDKPM-KGMLTGPVTMLQWSFVRDDQERAQTCRQIALALRDEVQDLEKAGIKVIQID 604
Query: 179 EPAMLNH--------PDEVPLAVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDIRV 228
EPA+ D + AVE+ A ++ ++ H+CYS + + + +
Sbjct: 605 EPAIREGLPLRRGEWADYLNWAVESFRIASSNVRDTTQIHTHMCYSEFNDIIEAVAALDA 664
Query: 229 TQFALEFANRQFRDMD-FLKKLSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPEW 287
++E + + +D F+K +G GV D+H+PR+ +E+V +KKA L PE
Sbjct: 665 DVISIETSRSRMELLDAFVKFRYPNAIGPGVYDIHSPRVPQEEEMVLLLKKARAVLPPEQ 724
Query: 288 LYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKE 321
L++NPDCGLK + L MV A + +R E
Sbjct: 725 LWVNPDCGLKTRGWKETRAALQTMVHAAQRLRAE 758
>pir||E81140 5-methyltetrahydropteroyltriglutamate- homocysteine
methyltransferase NMB0944 [imported] - Neisseria
meningitidis (group B strain MD58)
>gi|7226182|gb|AAF41350.1| (AE002445)
5-methyltetrahydropteroyltriglutamate-homocysteine
methyltransferase [Neisseria meningitidis MC58]
Length = 758
Score = 132 bits (330), Expect = 4e-30
Identities = 95/343 (27%), Positives = 165/343 (47%), Gaps = 26/343 (7%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
+ LP+LPT+ IGS+P+ + + + G++ D++ A+K V+ E E+ +D+
Sbjct: 423 LNLPLLPTTNIGSFPQTTEIRQARSAFKKGELSAADYEAAMKKEIALVVEEQEKLDLDVL 482
Query: 61 WDGEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWV 118
GE R++M E+F +SGF F YG V+ +G+ + E + + +
Sbjct: 483 VHGEAERNDMVEYFGELLSGFAFTQYGWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYA 542
Query: 119 RENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLD 178
+ T R + K +TGP TI +WSF + + +A +N E+ LE+ G IQ+D
Sbjct: 543 QSLTKRPM-KGMLTGPVTILQWSFVRNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQID 601
Query: 179 EPAMLNHPDEVPL-----------AVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDD 225
EPA+ + +PL A E+ + G + ++ H+CYS + +
Sbjct: 602 EPAIR---EGLPLKRADWDAYLNWAGESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAA 658
Query: 226 IRVTQFALEFANRQFRDMDFLKKLSG----KELGFGVVDVHNPRIETVDEIVRAIKKAFN 281
+ +E + DM+ L ++G GV D+H+PR+ T E+ ++KA
Sbjct: 659 MDADVITIETSRS---DMELLTAFGEFQYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIE 715
Query: 282 YLEPEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKELEK 324
+ E L++NPDCGLK + ++L M+ R +R EL K
Sbjct: 716 VVPVERLWVNPDCGLKTRGWKETLEQLQVMMNVTRKLRAELAK 758
>sp|O05564|METE_MYCLE 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE
METHYLTRANSFERASE (METHIONINE SYNTHASE, VITAMIN-B12
INDEPENDENT ISOZYME) (COBALAMIN-INDEPENDENT METHIONINE
SYNTHASE) >gi|2052110|emb|CAB08123.1| (Z94723) MetE
[Mycobacterium leprae]
Length = 760
Score = 132 bits (329), Expect = 6e-30
Identities = 93/332 (28%), Positives = 157/332 (47%), Gaps = 14/332 (4%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
+ LP LPT+ IGSYP+ + + + +I E ++ +K ++ E+ G+D+
Sbjct: 427 LHLPPLPTTTIGSYPQTSAIRKARAALQDAEIDEAEYISRMKKEVADAIKLQEQLGLDVL 486
Query: 61 WDGEMWRSEMTEHFTAKISGF--KFYGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWV 118
GE R++M ++F ++ GF G V+ +G+ + P+ +E ++
Sbjct: 487 VHGEPERNDMVQYFAEQLGGFFATQNGWVQSYGSRCVRPPILYGDVSRPHPMTIE-WITY 545
Query: 119 RENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLD 178
++ T + VK +TGP TI WSF +A I E L+ G IQ+D
Sbjct: 546 AQSLTDKPVKGMLTGPVTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIAIIQVD 605
Query: 179 EPAM-----LNHPDE---VPLAVEAINRAVKGI--KIKVGLHVCYSNYYLLADYFDDIRV 228
EPA+ L D+ + AV+A A G+ ++ H+CYS + + D+
Sbjct: 606 EPALRELLPLRRADQDEYLCWAVKAFRLATSGVADSTQIHTHLCYSEFGEVIGAIADLDA 665
Query: 229 TQFALEFANRQFRDMDFLKKLS-GKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPEW 287
++E A +D L + +G GV D+H+PR+ + DEI ++++ A + +
Sbjct: 666 DVTSIEAARSHMEVLDDLNAVGFANSIGPGVYDIHSPRVPSTDEIAKSLRAALKAIPMQR 725
Query: 288 LYINPDCGLKLLDRRIAYQKLVNMVKAVRIVR 319
L++NPDCGLK L NMV A R VR
Sbjct: 726 LWVNPDCGLKTRSVDEVSASLQNMVAAARQVR 757
>sp|P45331|METE_HAEIN 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE
METHYLTRANSFERASE (METHIONINE SYNTHASE, VITAMIN-B12
INDEPENDENT ISOZYME) (COBALAMIN-INDEPENDENT METHIONINE
SYNTHASE) >gi|1075341|pir||B64137
5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase (EC 2.1.1.14) - Haemophilus
influenzae (strain Rd KW20) >gi|1574556|gb|AAC23348.1|
(U32843)
5-methyltetrahydropteroyltriglutamate-homocysteine
methyltransferase (metE) [Haemophilus influenzae Rd]
Length = 756
Score = 131 bits (326), Expect = 1e-29
Identities = 93/338 (27%), Positives = 161/338 (47%), Gaps = 20/338 (5%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
+ LP+LPT+ IGS+P+ + + G++ D++ A+K V+R E +D+
Sbjct: 422 LNLPLLPTTNIGSFPQTTEIRHARASFKKGELSLADYEAAMKKEIEYVVRRQEELDLDVL 481
Query: 61 WDGEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWV 118
GE R++M E+F + GF F +G V+ +G+ + EP+ + +
Sbjct: 482 VHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQYA 541
Query: 119 RENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLD 178
+ T R ++K +TGP TI +WSF + + + ++ E+ LE G IQ+D
Sbjct: 542 QSLTNR-VMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQID 600
Query: 179 EPAM-----LNHPD---EVPLAVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDIRV 228
EPA+ L D + A EA + G++ ++ H+CYS + + +
Sbjct: 601 EPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIHTHMCYSEFNDILPAIAALDA 660
Query: 229 TQFALEFANRQFRDMDFLKKLSG----KELGFGVVDVHNPRIETVDEIVRAIKKAFNYLE 284
+E + DM+ L + ++G GV D+H+PR+ T E+ ++KA N +
Sbjct: 661 DVITIETSRS---DMELLTAFADFKYPNDIGPGVYDIHSPRVPTATEVEHLLRKALNVIP 717
Query: 285 PEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKEL 322
E L++NPDCGLK +L MV + +R EL
Sbjct: 718 KERLWVNPDCGLKTRGWTETIDQLKVMVDVTKKLRAEL 755
>gb|AAA23544.1| (M87625) cobalamin-independent methionine synthase [Escherichia
coli]
Length = 753
Score = 131 bits (325), Expect = 2e-29
Identities = 96/337 (28%), Positives = 160/337 (46%), Gaps = 24/337 (7%)
Query: 2 ELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPW 61
+LP PT+ IGS+P+ + + + G + +++ + + + E ER G+D+
Sbjct: 422 KLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVLV 481
Query: 62 DGEMWRSEMTEHFTAKISGFKFY--GPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVR 119
GE R++M E+F + GF F G V+ +G+ + + P+ +E W +
Sbjct: 482 HGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDISRPAPITVE---WAK 538
Query: 120 --ENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQL 177
++ T + VK +TGP TI WSF +E+ +A + E+ LE G IQ+
Sbjct: 539 YAQSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQI 598
Query: 178 DEPAM-----LNHPD---EVPLAVEA--INRAVKGIKIKVGLHVCYSNYYLLADYFDDIR 227
DEPA+ L D + VEA IN AV ++ H+CY + + D +
Sbjct: 599 DEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALD 658
Query: 228 VTQFALEFANRQFRDMDFLKKLSG----KELGFGVVDVHNPRIETVDEIVRAIKKAFNYL 283
+E + DM+ L+ E+G GV D+H+P + +V+ I +KKA +
Sbjct: 659 ADVITIETSRS---DMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRI 715
Query: 284 EPEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRK 320
E L++NPDCGLK L NMV+A + +R+
Sbjct: 716 PAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLRR 752
>sp|P25665|METE_ECOLI 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE
METHYLTRANSFERASE (METHIONINE SYNTHASE, VITAMIN-B12
INDEPENDENT ISOZYME) (COBALAMIN-INDEPENDENT METHIONINE
SYNTHASE) >gi|7427873|pir||A42863
5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase (EC 2.1.1.14) - Escherichia coli
(strain K-12) >gi|2367304|gb|AAC76832.1| (AE000458)
tetrahydropteroyltriglutamate methyltransferase
[Escherichia coli K12]
Length = 753
Score = 131 bits (325), Expect = 2e-29
Identities = 96/337 (28%), Positives = 160/337 (46%), Gaps = 24/337 (7%)
Query: 2 ELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPW 61
+LP PT+ IGS+P+ + + + G + +++ + + + E ER G+D+
Sbjct: 422 KLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVLV 481
Query: 62 DGEMWRSEMTEHFTAKISGFKFY--GPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVR 119
GE R++M E+F + GF F G V+ +G+ + + P+ +E W +
Sbjct: 482 HGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDISRPAPITVE---WAK 538
Query: 120 --ENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQL 177
++ T + VK +TGP TI WSF +E+ +A + E+ LE G IQ+
Sbjct: 539 YAQSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQI 598
Query: 178 DEPAM-----LNHPD---EVPLAVEA--INRAVKGIKIKVGLHVCYSNYYLLADYFDDIR 227
DEPA+ L D + VEA IN AV ++ H+CY + + D +
Sbjct: 599 DEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALD 658
Query: 228 VTQFALEFANRQFRDMDFLKKLSG----KELGFGVVDVHNPRIETVDEIVRAIKKAFNYL 283
+E + DM+ L+ E+G GV D+H+P + +V+ I +KKA +
Sbjct: 659 ADVITIETSRS---DMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRI 715
Query: 284 EPEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRK 320
E L++NPDCGLK L NMV+A + +R+
Sbjct: 716 PAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLRR 752
>gb|AAF33427.1| (AF233324) 94% identity with E. coli
5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase (METE) (SP:P25665) [Salmonella
typhimurium LT2]
Length = 754
Score = 131 bits (325), Expect = 2e-29
Identities = 96/337 (28%), Positives = 159/337 (46%), Gaps = 24/337 (7%)
Query: 2 ELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPW 61
+LP PT+ IGS+P+ + + + G + ++ + + + E ER G+D+
Sbjct: 422 KLPAWPTTTIGSFPQTTEIRGLRLDFKKGNLDANHYRTGIAEHIKQAIIEQERLGLDVLV 481
Query: 62 DGEMWRSEMTEHFTAKISGFKFY--GPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVR 119
GE R++M E+F + GF F G V+ +G+ + + P+ +E W +
Sbjct: 482 HGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPVVIGDISRPAPITVE---WAK 538
Query: 120 --ENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQL 177
++ T + VK +TGP TI WSF +E+ +A + E+ LE G IQ+
Sbjct: 539 YAQSLTDKPVKGMLTGPVTILCWSFPREDVTRETIAKQIALALRDEVADLEAAGIGIIQI 598
Query: 178 DEPAM-----LNHPD---EVPLAVEA--INRAVKGIKIKVGLHVCYSNYYLLADYFDDIR 227
DEPA+ L D + VEA IN AV + ++ H+CY + + D +
Sbjct: 599 DEPALREGLPLRRSDWDAYLEWGVEAFRINAAVAKDETQIHTHMCYCEFNDIMDSIAALD 658
Query: 228 VTQFALEFANRQFRDMDFLKKLSG----KELGFGVVDVHNPRIETVDEIVRAIKKAFNYL 283
+E + DM+ L+ E+G GV D+H+P + +V+ I +KKA +
Sbjct: 659 ADVITIETSRS---DMELLESFEAFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAQRI 715
Query: 284 EPEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRK 320
+ L++NPDCGLK L NMVKA +R+
Sbjct: 716 PAQRLWVNPDCGLKTRGWPETRAALANMVKAAHNLRQ 752
>pir||G81880 probable 5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase (EC 2.1.1.14) NMA1140 [imported] -
Neisseria meningitidis (group A strain Z2491)
>gi|7379833|emb|CAB84402.1| (AL162755) putative
5-methyltetrahydropteroyltriglutamate--homocysteine
methyltransferase [Neisseria meningitidis Z2491]
Length = 758
Score = 130 bits (324), Expect = 2e-29
Identities = 94/343 (27%), Positives = 164/343 (47%), Gaps = 26/343 (7%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
+ LP+LPT+ IGS+P+ + + + G++ D++ A+K V+ E E+ +D+
Sbjct: 423 LNLPLLPTTNIGSFPQTTEIRQARAAFKKGELSAADYEAAMKKEIALVVEEQEKLDLDVL 482
Query: 61 WDGEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWV 118
GE R++M E+F +SGF F YG V+ +G+ + E + + +
Sbjct: 483 VHGEAERNDMVEYFGELLSGFAFTQYGWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYA 542
Query: 119 RENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLD 178
+ T R + K +TGP TI +WSF + + +A +N E+ LE+ G IQ+D
Sbjct: 543 QSLTKRPM-KGMLTGPVTILQWSFVRNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQID 601
Query: 179 EPAMLNHPDEVPL-----------AVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDD 225
EPA+ + +PL A E+ + G + ++ H+CYS + +
Sbjct: 602 EPAIR---EGLPLKRADWDAYLNWAGESFRLSSTGCEDSTQIHTHMCYSEFNDILPAIAA 658
Query: 226 IRVTQFALEFANRQFRDMDFLKKLSG----KELGFGVVDVHNPRIETVDEIVRAIKKAFN 281
+ +E + DM+ L ++G GV D+H+PR+ T E+ ++KA
Sbjct: 659 MDADVITIETSRS---DMELLTAFGEFKYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIE 715
Query: 282 YLEPEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKELEK 324
+ E L++NPDCGLK + ++L M+ +R EL K
Sbjct: 716 VVPVERLWVNPDCGLKTRGWKETLEQLQVMMNVTHKLRAELAK 758
>gb|AAA67625.1| (M87049) 5-methyltetrahydropteroyltriglutamate- homocysteine
methyltransferase [Escherichia coli]
Length = 753
Score = 130 bits (324), Expect = 2e-29
Identities = 97/337 (28%), Positives = 158/337 (46%), Gaps = 24/337 (7%)
Query: 2 ELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPW 61
+LP PT+ IGS+P+ + + + G + +++ + + + E ER G+D+
Sbjct: 422 KLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVLV 481
Query: 62 DGEMWRSEMTEHFTAKISGFKFY--GPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVR 119
GE R++M E+F + GF F G V+ +G+ + + P+ +E W +
Sbjct: 482 HGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDISRPAPITVE---WAK 538
Query: 120 --ENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQL 177
++ T + VK +TGP TI WSF +E+ +A + E+ LE G IQ+
Sbjct: 539 YAQSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQI 598
Query: 178 DEPAM-----LNHPD---EVPLAVEA--INRAVKGIKIKVGLHVCYSNYYLLADYFDDIR 227
DEPA+ L D + VEA IN AV ++ H+CY + D D I
Sbjct: 599 DEPALRQGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFN---DIMDSIA 655
Query: 228 VTQFALEFANRQFRDMDFLKKLSG----KELGFGVVDVHNPRIETVDEIVRAIKKAFNYL 283
+ DM+ L+ E+G GV D+H+P + +V+ I +KKA +
Sbjct: 656 ALDRDVITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRI 715
Query: 284 EPEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRK 320
E L++NPDCGLK L NMV+A + +R+
Sbjct: 716 PAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLRR 752
>sp|O06584|METE_MYCTU 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE
METHYLTRANSFERASE (METHIONINE SYNTHASE, VITAMIN-B12
INDEPENDENT ISOZYME) (COBALAMIN-INDEPENDENT METHIONINE
SYNTHASE) >gi|7433424|pir||F70539 probable
5-methyltetrahydropteroyltriglutamate--homocysteine
methyltransferase - Mycobacterium tuberculosis (strain
H37RV) >gi|2117227|emb|CAB09044.1| (Z95585) metE
[Mycobacterium tuberculosis]
Length = 759
Score = 130 bits (324), Expect = 2e-29
Identities = 94/333 (28%), Positives = 155/333 (46%), Gaps = 16/333 (4%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
+ LP LPT+ IGSYP+ + G+I E ++ ++ V+ ER G+D+
Sbjct: 427 LHLPPLPTTTIGSYPQTSAIRVARAALRAGEIDEAEYVRRMRQEITEVIALQERLGLDVL 486
Query: 61 WDGEMWRSEMTEHFTAKISGFKFYGPVRVWGNAYFNKAAAVDKL--EYKEPLVLE-EFLW 117
GE R++M ++F +++GF + W +Y ++ L + P + E++
Sbjct: 487 VHGEPERNDMVQYFAEQLAGF--FATQNGWVQSYGSRCVRPPILYGDVSRPRAMTVEWIT 544
Query: 118 VRENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQL 177
++ T + VK +TGP TI WSF +A I E L+ G IQ+
Sbjct: 545 YAQSLTDKPVKGMLTGPVTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIAVIQV 604
Query: 178 DEPAM-----LNHPDEVPL---AVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDIR 227
DEPA+ L D+ AV A A G+ ++ H+CYS + + D+
Sbjct: 605 DEPALRELLPLRRADQAEYLRWAVGAFRLATSGVSDATQIHTHLCYSEFGEVIGAIADLD 664
Query: 228 VTQFALEFANRQFRDMDFLKKLS-GKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPE 286
++E A +D L + +G GV D+H+PR+ + +E+ +++ A + E
Sbjct: 665 ADVTSIEAARSHMEVLDDLNAIGFANGVGPGVYDIHSPRVPSAEEMADSLRAALRAVPAE 724
Query: 287 WLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVR 319
L++NPDCGLK + L NMV A R VR
Sbjct: 725 RLWVNPDCGLKTRNVDEVTASLHNMVAAAREVR 757
>gb|AAF82115.1|AF275676_1 (AF275676) cobalamin-independent methionine synthase [Aspergillus
nidulans]
Length = 774
Score = 129 bits (321), Expect = 5e-29
Identities = 90/341 (26%), Positives = 160/341 (46%), Gaps = 21/341 (6%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
++LP+ PT+ IGS+P+ + + G+I E++++ ++ V++ E G+D+
Sbjct: 438 IKLPLFPTTTIGSFPQTKEIRIQRNKFTKGEITAEEYEKFIEKEIAEVVKIQEELGLDVL 497
Query: 61 WDGEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWV 118
GE R++M ++F +++G+ F + V+ +G+ V + P+ ++E +
Sbjct: 498 VHGEPERNDMVQYFGERLTGYVFTTHAWVQSYGSRCVRPPIIVGDISRPAPMTVKESKYA 557
Query: 119 RENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLD 178
+++ + K +TGP T WSF + LA + E+ LE G IQ+D
Sbjct: 558 VSISSKPM-KGMLTGPITCLRWSFPRDDVHQSVQAQQLALALRDEVVDLEAAGVKVIQVD 616
Query: 179 EPAMLN---------HPDEVPLAVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDIR 227
EPA+ D + AV A + G+ ++ H CYS + D+F I
Sbjct: 617 EPALREGLPLRAGKEREDYLQWAVAAFRLSTSGVSDGTQIHSHFCYSEFQ---DFFHAIA 673
Query: 228 VTQFALEFANRQFRDMDFLK----KLSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYL 283
+ D LK + + +G GV D+H+PR+ + EI +++ YL
Sbjct: 674 ALDADVLSIENSKSDAKLLKVFIDEAYPRHIGPGVYDIHSPRVPSEQEIKDRVEEMLAYL 733
Query: 284 EPEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKELEK 324
PE L+INPDCGLK L N+V+A + R++ K
Sbjct: 734 RPEQLWINPDCGLKTRQWPETKAALSNLVQAAKYFREKYAK 774
>sp|O26869|Y775_METTH HYPOTHETICAL PROTEIN MTH775 >gi|7447490|pir||F69203
5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase (EC 2.1.1.14) - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2621866|gb|AAB85278.1| (AE000856)
cobalamin-independent methionine synthase
[Methanobacterium thermoautotrophicum]
Length = 308
Score = 128 bits (319), Expect = 8e-29
Identities = 106/314 (33%), Positives = 157/314 (49%), Gaps = 18/314 (5%)
Query: 8 TSVIGSYP-KPRW--LLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPWDGE 64
T+V+GSYP +PR L L G + + A A +R+ RAGVDI DG+
Sbjct: 3 TTVVGSYPTEPRGPETLGERLLNFFGSY------DRYRPAIEAAVRDQFRAGVDIISDGQ 56
Query: 65 MWRSEMTEHFTAKISGFKFYGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVRENTTR 124
+ R +M HF A I G K + + A ++ + + + L E+
Sbjct: 57 V-RGDMVGHFAAAIGGMKIEDGTSIIYSRITPPAGSIGAADLRYAYRILRGLTDDESRG- 114
Query: 125 EIVKVPITGPYTIAEWSFNEYYPD---KESFVMDLAKIINKELKTLEEHGATYIQLDEPA 181
VK ITGP T+ S E + D ++ VMD+A ++ E K L++ GA IQ+DEP
Sbjct: 115 --VKGIITGPSTMIYASRIEGFYDPQKRDRAVMDMAGVLKIEAKHLQDAGAAMIQIDEPF 172
Query: 182 MLNHPDEVPLAVEAINRAVKGIKIKVGLHVCYSNYYLLADYFDDIRVTQFALEFANRQFR 241
+ ++ A AI+ G+ I+V LHVC +L+D +V LEFA R
Sbjct: 173 LSTGIVDMKTAGRAIDHIAAGLDIEVSLHVCGDIRNVLSDLLR-FKVDVLDLEFAGRPSN 231
Query: 242 DMDFLKKLSG-KELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPEWLYINPDCGLKLLD 300
+K G K +GFG VD R+E+++EI IK+ + + E LYI+PDCG++ L
Sbjct: 232 LEVLEEKWRGDKGVGFGCVDTTTERVESMEEIRNLIKRGADIVGEENLYIDPDCGMRKLP 291
Query: 301 RRIAYQKLVNMVKA 314
R+ A+ KL NMV A
Sbjct: 292 RKAAFSKLRNMVMA 305
>gb|AAF33834.1| (AF226997) methionine synthase [Cladosporium fulvum]
Length = 768
Score = 122 bits (304), Expect = 5e-27
Identities = 90/341 (26%), Positives = 160/341 (46%), Gaps = 27/341 (7%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
+ LP PT+ IGS+P+ + + G+I E+++ E ++ ++ E G+D+
Sbjct: 433 LNLPEFPTTTIGSFPQTKEIRVQRNKFTKGEITEKEYDEFIEAEIRDNIKIQEELGLDVY 492
Query: 61 WDGEMWRSEMTEHFTAKISGFKF--YGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWV 118
GE R++M ++F ++ G+ F + V+ +G+ V + +P+ ++E +
Sbjct: 493 VHGEPERNDMVQYFGERLDGYVFTTHAWVQSYGSRCVRPPILVGDVSRPKPMTVKESKYA 552
Query: 119 RENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLD 178
+ +++ +K +TGP T WSF + LA + E+ L+ G IQ+D
Sbjct: 553 -VSQSKKPMKGMLTGPITCLRWSFPRDDVHQSIQAAQLALALRDEVVDLQNAGVAVIQID 611
Query: 179 EPAMLNHPDEVPL------------AVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFD 224
EPA+ + +PL AV++ + G++ ++ H CYS + D+F
Sbjct: 612 EPALR---EGLPLRSGSEREKYLQWAVDSFKLSAAGVEDATQIHSHFCYSEFQ---DFFH 665
Query: 225 DIRVTQFALEFANRQFRDMDFLKKLSGKE----LGFGVVDVHNPRIETVDEIVRAIKKAF 280
I + D LK ++ +G GV D+H+PRI + EI I++
Sbjct: 666 AIAALDADVLSIENSKSDAKLLKVFIDEKYPRHIGPGVYDIHSPRIPSEQEIKDRIQEML 725
Query: 281 NYLEPEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKE 321
YL PE L+INPDCGLK + L +MV A + R +
Sbjct: 726 QYLTPEQLWINPDCGLKTRQWKETKDALAHMVSAAQHFRSQ 766
>sp|Q39586|METE_CHLRE 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE
METHYLTRANSFERASE (METHIONINE SYNTHASE, VITAMIN-B12
INDEPENDENT ISOZYME) (COBALAMIN-INDEPENDENT METHIONINE
SYNTHASE) >gi|2129456|pir||S65083
5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase (EC 2.1.1.14) - Chlamydomonas
reinhardtii >gi|1017759|gb|AAC49178.1| (U36197)
cobalamin-independent methionine synthase [Chlamydomonas
reinhardtii] >gi|1587968|prf||2207381A Met synthase
[Chlamydomonas reinhardtii]
Length = 814
Score = 122 bits (302), Expect = 8e-27
Identities = 95/348 (27%), Positives = 164/348 (46%), Gaps = 24/348 (6%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
++L PT+ IGS+P+ + R+ + + G++ + +++ + + E G+D+
Sbjct: 435 LQLAAFPTTTIGSFPQTAEVRRLRQQLKSGRLTQAEYEGLIAGHIAHAVGVQEALGIDVL 494
Query: 61 WDGEMWRSEMTEHFTAKISGFKFY--GPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWV 118
GE R++M E+F ++ G F G V+ +G+ VD + Y+ P E+ V
Sbjct: 495 VHGEAERTDMVEYFGMQLGGMLFTRAGWVQSYGSRCVRPPLVVDDITYRGPSTCWEYK-V 553
Query: 119 RENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLD 178
T + VK +TGP TI WSF + + M L + +E+ LE G T IQ+D
Sbjct: 554 AIPTAAQPVKGLLTGPVTILNWSFPRKDISRAAQAMQLGLALRQEVAALEAAGCTIIQVD 613
Query: 179 EPAMLNHPDEVPLAVEA----INRAVKGIKIKVGL---------HVCYSNYYLLADYFDD 225
+PA+ + +PL E ++ AV ++ G+ H+CYS++ + D
Sbjct: 614 DPAL---REGLPLNGERWASYLSWAVDAFRLCTGVAAAGTQVVTHLCYSDFQDILPAIDR 670
Query: 226 IRVTQFALEFANRQFRDMDFLKKLS-GKELGFGVVDVHNPRIETVDEIVRAIKKAFN--Y 282
+ +E + M L G+++G GV DVH+P + +V+ I I+ +
Sbjct: 671 MDADVLTIENSRSDKPMMAALAAAGYGRDIGPGVYDVHSPVVPSVEFIKSRIRSFVDSGI 730
Query: 283 LEPEW--LYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKELEKEGRA 328
L + +++NPDCGLK L NMV+A R EL+ G A
Sbjct: 731 LSGRYDRIWVNPDCGLKTRGWPETIAALANMVEAAAQARAELQLAGGA 778
>sp|Q58868|YE73_METJA HYPOTHETICAL PROTEIN MJ1473 >gi|2127725|pir||H64483
5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase (EC 2.1.1.14) - Methanococcus
jannaschii >gi|1592112|gb|AAB99479.1| (U67588)
5-methyltetrahydropteroyltriglutamate-homocysteine
methyltransferase (metE) [Methanococcus jannaschii]
Length = 311
Score = 121 bits (301), Expect = 1e-26
Identities = 98/333 (29%), Positives = 177/333 (52%), Gaps = 49/333 (14%)
Query: 8 TSVIGSYP----KPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPWDG 63
T+V+GSYP + +L ++ K+ L +++K A++ A +++ +AGV+I DG
Sbjct: 6 TTVVGSYPVVKKEETFLDKVKKVFGL----YDEYKYAIERA----VKDQVKAGVNIISDG 57
Query: 64 EMWRSEMTEHFTAKISGFKFYGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVRE--- 120
++ R +M E FT + GF + V ++E+ +P+ L++ L+ +
Sbjct: 58 QV-RGDMVEIFTNNMYGF--------------DGKRVVGRVEFIKPITLKDILYAKSIAK 102
Query: 121 --NTTREIVKVPITGPYTIAEW-SFNEYYPDK--ESFVMDLAKIINKELKTLEEHGATYI 175
N EI + ITGP TIA Y D E+ + D+AK + KE++ L++H I
Sbjct: 103 KLNPNVEIKGI-ITGPCTIASSVRVESCYSDNRDENLIYDIAKALRKEVEALKKH-VPII 160
Query: 176 QLDEPAMLNHPDEVPLAVEAINRAVKGIKIKVGLHVCYSNYYLLADYFDDIRVTQFALEF 235
Q+DEP + + +A +AI+ V G+ IK +HVC N Y + D + V EF
Sbjct: 161 QIDEPILSTGMYDFDVARKAIDIIVDGLNIKFAMHVC-GNVYNIIDELNKFNVDILDHEF 219
Query: 236 ANRQFRDMDFLKKLSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLE---------PE 286
A+ + +++ L+ + K++GFG V+ ++E+V+EI I++ L+ +
Sbjct: 220 ASNK-KNLVILESME-KKVGFGCVNTKVKKVESVEEIKSLIEEGIEILKNNEKLNKNLSD 277
Query: 287 WLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVR 319
+ I+PDCG++LL +A+ KL NMV+A ++++
Sbjct: 278 NILIDPDCGMRLLPIDVAFNKLKNMVEATKLIK 310
>sp|P55299|Y775_METTM HYPOTHETICAL 34.6 KDA PROTEIN IN TYSY 5'REGION (ORF1)
>gi|2127678|pir||S62191
5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase (EC 2.1.1.14) - Methanobacterium
thermoautotrophicum (strain Marburg)
>gi|1103707|emb|CAA63062.1| (X92082)
cobalamin-independent methionine synthase
[Methanobacterium thermoautotrophicum]
Length = 319
Score = 118 bits (293), Expect = 9e-26
Identities = 103/315 (32%), Positives = 150/315 (46%), Gaps = 20/315 (6%)
Query: 8 TSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPWDGEMWR 67
T+V+GSYP + R I D A + A +R+ RAGVDI DG++ R
Sbjct: 13 TTVVGSYPATPREPETLRERISSFIGSYD---ACRPAIETAVRDQVRAGVDIISDGQV-R 68
Query: 68 SEMTEHFTAKISGFKFYGPVRVWGNAYFNKAAAV--DKLEYKEPLVLEEFLWVRENTTRE 125
+M HF + G V + + A ++ D L Y+ ++ R+ T E
Sbjct: 69 GDMVGHFAEAMGGMMVKDGVSIIFSRITPPAGSIGSDDLIYRIKIL-------RKLTDDE 121
Query: 126 I--VKVPITGPYTIAEWSFNE--YYPDK-ESFVMDLAKIINKELKTLEEHGATYIQLDEP 180
VK ITGP T+A S E Y P K E +MD+A + E + L++ GA IQ+DEP
Sbjct: 122 SKGVKGIITGPSTMAHASKIEGFYSPQKRERAIMDMADALRVEAEHLQDAGALMIQIDEP 181
Query: 181 AMLNHPDEVPLAVEAINRAVKGIKIKVGLHVCYSNYYLLADYFDDIRVTQFALEFANRQF 240
+ ++ A A+ R + + V LHVC ++ + V LEFA R
Sbjct: 182 FLSTGMVDMGTARRAVKRISGALDVDVSLHVCGDISGVIGELLT-FPVDMLDLEFAGRPS 240
Query: 241 RDMDFLKKLSG-KELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPEWLYINPDCGLKLL 299
+K G K+LGFG VD +E+ D I I++ + + LYI+PDCG++ L
Sbjct: 241 NLEVLAEKWRGDKKLGFGCVDTSTEVVESTDTIKNLIERGADIAGEDNLYIDPDCGMRKL 300
Query: 300 DRRIAYQKLVNMVKA 314
R A+ KL NMVKA
Sbjct: 301 PREAAFSKLRNMVKA 315
>pir||T39194 5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase (EC 2.1.1.14) - fission yeast
(Schizosaccharomyces pombe) >gi|6014428|emb|CAB57427.1|
(AL121764)
5-methyltetrahydropteroyltriglutamate--homocystei
methyltransferase(ec 2.1.1.14) [Schizosaccharomyces
pombe]
Length = 764
Score = 118 bits (293), Expect = 9e-26
Identities = 88/335 (26%), Positives = 154/335 (45%), Gaps = 18/335 (5%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
++LP+ PT+ IGS+P+ + + G I +E++ ++ V++ E G+D+
Sbjct: 432 LKLPLFPTTTIGSFPQTKEIRVTRNRFAKGLISQEEYDAFIRKEISDVVKFQEEVGLDVL 491
Query: 61 WDGEMWRSEMTEHFTAKISGFKFY--GPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWV 118
GE R++M ++F ++ GF F G V+ +G+ V + P+ ++E +
Sbjct: 492 VHGEPERNDMVQYFGERMEGFVFTVNGWVQSYGSRCVRPPIIVGDVYRPAPMTVKESQYA 551
Query: 119 RENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLD 178
+ T++ + K +T P TI WSF +A + E+ LE+ G IQ D
Sbjct: 552 QSITSKPM-KGMLTAPITILRWSFPRDDVHDSVQAQQIALGLRDEVLDLEKAGIKVIQCD 610
Query: 179 EPAM--------LNHPDEVPLAVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDIRV 228
EPA+ + + A++A A ++ ++ H CYS++ D FD I+
Sbjct: 611 EPALREGLPLRRAEWDEYLKWAIDAFRLATAAVQDDTQIHSHFCYSDFN---DIFDAIQR 667
Query: 229 TQFALEFANRQFRDMDFLKKLSGKE--LGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPE 286
+ DM L LS +G G+ D+H+PR+ V E I ++ +
Sbjct: 668 LDADVVSIENSKSDMKLLNVLSRYTSCIGPGLFDIHSPRVPPVSEFKERIDAIVKHVPKD 727
Query: 287 WLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKE 321
L++NPDCGLK L NM+ A R R++
Sbjct: 728 HLWLNPDCGLKTRGWPETTADLKNMIAAAREAREQ 762
>pir||S47051 hypothetical protein 1 - Xanthobacter sp >gi|520948|emb|CAA56241.1|
(X79863) orf1 [Xanthobacter sp. Py2]
Length = 376
Score = 114 bits (283), Expect = 1e-24
Identities = 96/368 (26%), Positives = 160/368 (43%), Gaps = 47/368 (12%)
Query: 6 LPTSVIGSYPKPRWLLRMYKLRELG--KIPEEDFKEAVKDASVAVLREHERAGVDIPWDG 63
+PTS++G+YP PRW + G + P+ +E+++DA A+ R+ ERAG+DI DG
Sbjct: 10 IPTSMVGNYPNPRWWDAQFARTWTGDQEPPDALIQESLEDAVAAIARDQERAGLDIISDG 69
Query: 64 EMWRSEMTE---HFTAKISGFKFYGPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVRE 120
+ E ++ + G+ G + A + + ++ E +
Sbjct: 70 RVHGDNYAEQALYYYYRRLGYDLKGGYLGFPIYSRLHAGTLTGEVRRHGAIMVEQAKALK 129
Query: 121 NTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDEP 180
T + KV TG + + + + +Y M +AK IN++++ ++ G +IQ+DE
Sbjct: 130 KATGKPTKVQYTGVQALTQATNDLHYKSSRDRAMAIAKAINEDIREVDALGVDFIQIDEF 189
Query: 181 AMLNHPDEVPLAVEAINRAVKGIK-IKVGLHVCYSNYYLLADYFDD-------------- 225
++ A+EA N AV G+K K+ HVC+ N+ Y+ D
Sbjct: 190 TWPYFFED--WAIEAFNAAVDGVKNAKIIAHVCWGNWGGTPAYYPDETAASGEIFDLTKR 247
Query: 226 -------------------IRVTQFALEFANRQFRDMDFLKKLSGKEL------GFGVVD 260
R+ LE R+ D+ L + L GV+D
Sbjct: 248 KAEATKATATGSIVPKAYEARLDVLNLESCGRRSDDLSGLHVMKNHPLPDNVSFWAGVID 307
Query: 261 VHNPRIETVDEIVRAIKKAFNYLEPEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRK 320
V + ET DE+ I++ + + L + DCGL LL R IA KL +V+ +IVR
Sbjct: 308 VKSTITETADEVANRIRRLLEIVPADRLGVTTDCGLILLQRYIAQDKLHALVEGTKIVRA 367
Query: 321 ELEKEGRA 328
EL K +A
Sbjct: 368 ELAKAKQA 375
>pir||T42529 probable 5-methyltetrahydropteroyltriglutamate--homocysteine
S-methyltransferase (EC 2.1.1.14) - fission yeast
(Schizosaccharomyces pombe) (fragment)
>gi|1749542|dbj|BAA13829.1| (D89167) similar to
Saccharomyces cerevisiae
5-methyltetrahydropteroyltriglutamate-homocysteine
s-methyltransferase, SWISS-PROT Accession Number P05694
[Schizosaccharomyces pombe]
Length = 454
Score = 111 bits (276), Expect = 9e-24
Identities = 84/318 (26%), Positives = 149/318 (46%), Gaps = 20/318 (6%)
Query: 1 MELPILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIP 60
++LP+ PT+ IGS+P+ + + G I +E++ ++ V++ E G+D+
Sbjct: 143 LKLPLFPTTTIGSFPQTKEIRVTRNRFAKGLISQEEYDAFIRKEISDVVKFQEEVGLDVL 202
Query: 61 WDGEMWRSEMTEHFTAKISGFKFY--GPVRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWV 118
GE R++M ++F ++ GF F G V+ +G+ V + P+ ++E +
Sbjct: 203 VHGEPERNDMVQYFGERMEGFVFTVNGWVQSYGSRGVRPPIIVGDVYRPAPMTVKESQYA 262
Query: 119 RENTTREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLD 178
+ T++ + K +T P TI WSF +A + E+ LE+ G IQ D
Sbjct: 263 QSITSKPM-KGMLTAPITILRWSFPRDDVHDSVQAQQIALGLRDEVLDLEKAGIKVIQCD 321
Query: 179 EPAM--------LNHPDEVPLAVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDIRV 228
EPA+ + + A++A A ++ ++ H CYS++ D FD I+
Sbjct: 322 EPALREGLPPRRAEWDEYLKWAIDAFRLATAAVQDDTQIHSHFCYSDFN---DIFDAIQR 378
Query: 229 TQFALEFANRQFRDMDFLKKLSGKE--LGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPE 286
+ DM L LS +G G+ D+H+PR+ V E I ++ +
Sbjct: 379 LDADVVSIENSKSDMKLLNVLSRYTSCIGPGLFDIHSPRVPPVSEFKERIDAIVKHVPKD 438
Query: 287 WLYINPDCGLKLLDRRIA 304
L++NPDCGL+ D R+A
Sbjct: 439 HLWLNPDCGLE--DPRMA 454
>gb|AAD00267.1| (U77388) cobalamin independent methionine synthase [Chlamydomonas
moewusii]
Length = 707
Score = 98.3 bits (241), Expect = 1e-19
Identities = 96/347 (27%), Positives = 152/347 (43%), Gaps = 43/347 (12%)
Query: 7 PTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPWDGEMW 66
PTS IGS+P+ + R G + E ++ + + E +D+ GE
Sbjct: 339 PTSTIGSFPQTAEVRRARAQLTKGLLTLEQYQARMDALITHAIGVQEGLDIDVLVHGEAE 398
Query: 67 RSEMTEHFTAKISGFKFYGP---VRVWGNAYFNKAAAVDKLEYKEPLVLEEFLWVRENT- 122
R++M E+F ++ G + V+ +G+ Y VD P+ + E W T
Sbjct: 399 RTDMVEYFGEQLEGLTAFTANAWVQSYGSRYVRPHHCVDVA--CAPMTVRE--WRSSQTP 454
Query: 123 --TREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDEP 180
T VK +TGP TI WSF + M LA + +Q+DEP
Sbjct: 455 ACTGRFVKGMLTGPVTILNWSFPRKDVSRRVQAMQLALACARRWTRCR-----IVQVDEP 509
Query: 181 AMLNHPDEVPL-----------AVEA--INRAVKGIKIKVGLHVCYSNYYLLADYFDDIR 227
A+ + +PL AV+A ++ AV +++ H+CYSN+ + DD+
Sbjct: 510 ALR---EGLPLKNERWQSYLTWAVDAFRLSTAVARPDVQIVTHLCYSNFEDILQAVDDMD 566
Query: 228 VTQFALEFANRQFRDMDFLKKLSG----KELGFGVVDVHNPRIETVDEIVRAIKKAFNYL 283
+E N + D + ++ LS K+LG GV DVH+P + V+ V I
Sbjct: 567 ADVLTIE--NSRSGD-EMVRALSNAGYCKDLGPGVYDVHSPVVPPVEWFVDKIASFVATC 623
Query: 284 E-----PEWLYINPDCGLKLLDRRIAYQKLVNMVKAVRIVRKELEKE 325
E P+ L++NPDCGLK L NMV+A +I+R+ K+
Sbjct: 624 EQFKDCPDRLWVNPDCGLKTRAWSEVIPALRNMVQAAQIMRERHAKQ 670
>gb|AAF26735.1| (AF220054) methionine synthase [Coffea arabica]
Length = 218
Score = 87.8 bits (214), Expect = 2e-16
Identities = 60/212 (28%), Positives = 98/212 (45%), Gaps = 12/212 (5%)
Query: 123 TREIVKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDEPAM 182
T+ +K +TGP TI WSF + +A I E++ LE+ G IQ+DE A+
Sbjct: 3 TQSPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGINVIQIDEAAL 62
Query: 183 --------LNHPDEVPLAVEAINRAVKGIK--IKVGLHVCYSNYYLLADYFDDIRVTQFA 232
H + AV + G++ ++ H+CYSN+ + D+
Sbjct: 63 REGLPLRKAEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVIT 122
Query: 233 LEFANRQFRDMDFLKK--LSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPEWLYI 290
+E + + + ++ G +G GV D+H+PRI + +EI + K L+ L++
Sbjct: 123 IENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRVNKMLAVLDTNILWV 182
Query: 291 NPDCGLKLLDRRIAYQKLVNMVKAVRIVRKEL 322
NPDCGLK L NMV A + +R +L
Sbjct: 183 NPDCGLKTRKYAEVKPALQNMVAAAKQIRTQL 214
>pir||B75138 tetrahydropteroyltriglutamate methyltransferase PAB2361 -
Pyrococcus abyssi (strain Orsay)
>gi|5458330|emb|CAB49819.1| (AJ248285)
TETRAHYDROPTEROYLTRIGLUTAMATE METHYLTRANSFERASE
[Pyrococcus abyssi]
Length = 313
Score = 79.6 bits (193), Expect = 5e-14
Identities = 74/311 (23%), Positives = 144/311 (45%), Gaps = 18/311 (5%)
Query: 5 ILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPWDGE 64
++ ++IGS P+P L + +L +G++ EE +EA +D + ++AG+ + DG
Sbjct: 4 LIVPALIGSLPRPVGLAKKIELYSIGRLSEEKLEEAYRDYTRRAFENLKKAGIKVVTDGL 63
Query: 65 MWRSEMTEHFTAKISGFKFYGPVRVWGNAYFNKAAAV-DKLEYKEPLVLEEFLWVRENTT 123
++ F I G + G + + N +F ++ V +L KE + E++ V ++
Sbjct: 64 YRWDDIFNPFIRFIEGVELNGLFKFYENNFFYRSPVVKGELSLKEN-PIPEWISVAQDIK 122
Query: 124 REI-----VKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLD 178
E+ +K + GP T+A S NE+Y + V +I + +K L+ + ++L
Sbjct: 123 DEVYPEATLKAVLPGPVTLAYHSINEHYKTLDELVEAYKDVIAELIKELD---VSIVELQ 179
Query: 179 EPAMLNHPDEVPL-AVEAINRAVKGIKIKVGLHVCYSNYYLLADYFDDIRVTQFALEFAN 237
EPA+ + AVE ++R V I+ + + YF +V + N
Sbjct: 180 EPALAAELSKATREAVEHVSRDVAKSAIE---ELAKIKRLWVVTYFGTPQVVPRNV-ILN 235
Query: 238 RQFRDMDFLKKLSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPEWLYINPDCGLK 297
+ + L G E+G G+V+ ++E D + ++K E + +Y+ P+ L
Sbjct: 236 VDLVEGRIPEGLEG-EVGLGIVNARETKMERADRLRDKLRKYVR--EFDVIYVTPNTLLD 292
Query: 298 LLDRRIAYQKL 308
L +A++KL
Sbjct: 293 FLPESVAWRKL 303
>pir||G71103 hypothetical protein PH1090 - Pyrococcus horikoshii
>gi|3257506|dbj|BAA30189.1| (AP000004) 309aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 309
Score = 71.4 bits (172), Expect = 1e-11
Identities = 69/305 (22%), Positives = 134/305 (43%), Gaps = 14/305 (4%)
Query: 9 SVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPWDGEMWRS 68
++IGS P+P L + +L +G++ EE +EA + + ++A + + DG
Sbjct: 5 ALIGSLPRPIGLAKKIELYSIGRLSEEKLEEAYRSYTRKAFENLKKARIKVITDGLYRWD 64
Query: 69 EMTEHFTAKISGFKFYGPVRVWGNAYFNKAAAV-DKLEYKE---PLVLEEFLWVRENTTR 124
++ I G + G + + N +F ++ V +L KE P + ++E+
Sbjct: 65 DIFNPLIRFIDGVEVNGLFKFYENNFFYRSPVVRGELSLKENPLPGWISIAQEIKEDVYP 124
Query: 125 EI-VKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLDEPAML 183
E +K + GP T+A S NEYY D A ++ + +K L+ ++L EP++
Sbjct: 125 EAELKAVLPGPVTLAYHSINEYYKDLNELTEAYATVLRELIKELD---VKIMELQEPSLA 181
Query: 184 NHPDEVPLAVEAINRAVKGIKIKVGLHVCYSNYYLLADYFDDIRVTQFALEFANRQFRDM 243
+ E+ K + KV + + YF +V + N +
Sbjct: 182 AELSKA--TRESEEHVSKEVAKKVIEDLARVKKLWVVTYFGTPQVVPKDV-ILNIDLIEG 238
Query: 244 DFLKKLSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPEWLYINPDCGLKLLDRRI 303
+ ++ G E+G G+V+ ++E D + ++K E +Y+ P+ L L R+
Sbjct: 239 NVPDEVKG-EVGLGIVNARETKMERADRLKDKLRKYIRKFEK--VYVTPNTLLDFLPERV 295
Query: 304 AYQKL 308
A++KL
Sbjct: 296 AWKKL 300
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.320 0.140 0.417
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 130652572
Number of Sequences: 2977
Number of extensions: 5611594
Number of successful extensions: 13182
Number of sequences better than 1.0e-10: 46
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 42
Number of HSP's that attempted gapping in prelim test: 13022
Number of HSP's gapped (non-prelim): 77
length of query: 338
length of database: 189,106,746
effective HSP length: 54
effective length of query: 284
effective length of database: 156,788,448
effective search space: 44527919232
effective search space used: 44527919232
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 165 (68.7 bits)