BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0632 (celB-like) DE:Endoglucanase
(514 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||E75142 endoglucanase PAB0632 - Pyrococcus abyssi (strain Or... 1088 0.0
pir||E71059 probable endo-1,4-beta-glucanase - Pyrococcus horiko... 424 e-117
pir||E82759 endo-1,4-beta-glucanase XF0818 [imported] - Xylella ... 321 1e-86
sp|P19487|GUNA_XANCP MAJOR EXTRACELLULAR ENDOGLUCANASE PRECURSOR... 315 7e-85
emb|CAC18529.1| (AJ304415) extracellular endoglucanase (ENGXCA p... 315 7e-85
sp|P23548|GUN_PAEPO ENDOGLUCANASE (ENDO-1,4-BETA-GLUCANASE) (CEL... 285 1e-75
pir||A35136 cellulase (EC 3.2.1.4) - Bacillus polymyxa 285 1e-75
sp|P54583|GUN1_ACICE ENDOGLUCANASE E1 PRECURSOR (ENDO-1,4-BETA-G... 264 2e-69
pdb|1ECE|A Chain A, Acidothermus Cellulolyticus Endocellulase E1... 264 2e-69
pdb|1C0D|A Chain A, Endocellulase E1 From Acidothermus Celluloly... 262 8e-69
emb|CAA44467.2| (X62582) Cellulase [Clavibacter michiganensis] 224 2e-57
sp|P10474|GUNB_CALSA ENDOGLUCANASE/EXOGLUCANASE B PRECURSOR [INC... 203 3e-51
pir||A43802 cellulase (EC 3.2.1.4) / cellulose 1,4-beta-cellobio... 203 3e-51
gb|AAD30364.1|AF078739_2 (AF078739) CelB [Caldicellulosiruptor s... 203 3e-51
dbj|BAA22939.1| (AB008029) beta-glucanase [thermophilic anaerobe... 201 1e-50
emb|CAB76938.1| (AJ275975) 1,4-beta-cellobiohydrolase [Clostridi... 201 2e-50
sp|P50400|GUND_CELFI ENDOGLUCANASE D PRECURSOR (ENDO-1,4-BETA-GL... 192 6e-48
pir||B47093 cellulase (EC 3.2.1.4) CenD - Cellulomonas fimi 192 6e-48
sp|Q05332|GUNG_CLOTM ENDOGLUCANASE G PRECURSOR (EGG) (ENDO-1,4-B... 167 3e-40
emb|CAB06784.1| (Z86104) endo-1,4-beta-glucanase [Anaerocellum t... 166 6e-40
sp|P04956|GUNB_CLOTM ENDOGLUCANASE B PRECURSOR (EGB) (ENDO-1,4-B... 166 8e-40
gb|AAD48494.1|AF172345_1 (AF172345) endo-1,4-beta-glucanase [Cel... 155 8e-37
pir||B82761 extracellular endoglucanase precursor XF0810 [import... 118 2e-25
>pir||E75142 endoglucanase PAB0632 - Pyrococcus abyssi (strain Orsay)
>gi|5458365|emb|CAB49854.1| (AJ248285) Endoglucanase
[Pyrococcus abyssi]
Length = 514
Score = 1088 bits (2784), Expect = 0.0
Identities = 514/514 (100%), Positives = 514/514 (100%)
Query: 1 MEIKLFCVFIVFIILFSPFVIALSYPDVNYTAENGIIFVQNVTTGEKKPLYLHGVSWFGF 60
MEIKLFCVFIVFIILFSPFVIALSYPDVNYTAENGIIFVQNVTTGEKKPLYLHGVSWFGF
Sbjct: 1 MEIKLFCVFIVFIILFSPFVIALSYPDVNYTAENGIIFVQNVTTGEKKPLYLHGVSWFGF 60
Query: 61 ELKDHVVYGLDKRNWKDILKDVKRLGFNAIRLPFCSESIRPDTRPSPERINYELNPDLKN 120
ELKDHVVYGLDKRNWKDILKDVKRLGFNAIRLPFCSESIRPDTRPSPERINYELNPDLKN
Sbjct: 61 ELKDHVVYGLDKRNWKDILKDVKRLGFNAIRLPFCSESIRPDTRPSPERINYELNPDLKN 120
Query: 121 LTSLEIMEKIIEYANSIGLYILLDYHRIGCEEIEPLWYTENYSEEQYIKDWIFLAKRFGK 180
LTSLEIMEKIIEYANSIGLYILLDYHRIGCEEIEPLWYTENYSEEQYIKDWIFLAKRFGK
Sbjct: 121 LTSLEIMEKIIEYANSIGLYILLDYHRIGCEEIEPLWYTENYSEEQYIKDWIFLAKRFGK 180
Query: 181 YPNVIGADIKNEPHGEAGWGTGDERDFRLFAEKVGREILKVAPHWLIFVEGTQYTHVPNI 240
YPNVIGADIKNEPHGEAGWGTGDERDFRLFAEKVGREILKVAPHWLIFVEGTQYTHVPNI
Sbjct: 181 YPNVIGADIKNEPHGEAGWGTGDERDFRLFAEKVGREILKVAPHWLIFVEGTQYTHVPNI 240
Query: 241 DEIIEKKGWWTFWGENLMGVKDYPVRLPRGKVVYSPHVYGPSVYMMDYFKSPDFPNNMPI 300
DEIIEKKGWWTFWGENLMGVKDYPVRLPRGKVVYSPHVYGPSVYMMDYFKSPDFPNNMPI
Sbjct: 241 DEIIEKKGWWTFWGENLMGVKDYPVRLPRGKVVYSPHVYGPSVYMMDYFKSPDFPNNMPI 300
Query: 301 IWETHFGYLTDLNYTLVIGEWGGNYEGLDKVWQDAFVKWLIKKKIYNFFYWCLNPESGDT 360
IWETHFGYLTDLNYTLVIGEWGGNYEGLDKVWQDAFVKWLIKKKIYNFFYWCLNPESGDT
Sbjct: 301 IWETHFGYLTDLNYTLVIGEWGGNYEGLDKVWQDAFVKWLIKKKIYNFFYWCLNPESGDT 360
Query: 361 GGIFLDDWKTVNWEKMRVIYRLIKAANPEFEEPLYIILKTNATTSILGVGERIRIYWYTN 420
GGIFLDDWKTVNWEKMRVIYRLIKAANPEFEEPLYIILKTNATTSILGVGERIRIYWYTN
Sbjct: 361 GGIFLDDWKTVNWEKMRVIYRLIKAANPEFEEPLYIILKTNATTSILGVGERIRIYWYTN 420
Query: 421 GKVIDSNFAHSSEGEMNITVTKSMTLYIIVKKGNQTLRKELKLYVIGGNYGSNISTTQLV 480
GKVIDSNFAHSSEGEMNITVTKSMTLYIIVKKGNQTLRKELKLYVIGGNYGSNISTTQLV
Sbjct: 421 GKVIDSNFAHSSEGEMNITVTKSMTLYIIVKKGNQTLRKELKLYVIGGNYGSNISTTQLV 480
Query: 481 TPKKGGERISTSLKLAISLLFILLFVWYLLREKH 514
TPKKGGERISTSLKLAISLLFILLFVWYLLREKH
Sbjct: 481 TPKKGGERISTSLKLAISLLFILLFVWYLLREKH 514
>pir||E71059 probable endo-1,4-beta-glucanase - Pyrococcus horikoshii
>gi|3257588|dbj|BAA30271.1| (AP000005) 458aa long
hypothetical endo-1,4-beta-glucanase [Pyrococcus
horikoshii]
Length = 458
Score = 424 bits (1078), Expect = e-117
Identities = 210/397 (52%), Positives = 272/397 (67%), Gaps = 23/397 (5%)
Query: 28 VNYTAENGIIFVQNVTTGEKKPLYLHGVSWFGFELKDHVVYGLDKRNWKDILKDVKRLGF 87
+ Y I++ NVT+GE+ P++L GV+WFGFE +HVV+GL KRNW+D+L +K LGF
Sbjct: 39 IYYEVRGDTIYMINVTSGEETPIHLFGVNWFGFETPNHVVHGLWKRNWEDMLLQIKSLGF 98
Query: 88 NAIRLPFCSESIRPDTRPSPERINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDYHR 147
NAIRLPFC+ES++P T+P I+Y NPDL+ L SL+IMEKII+ A +G+++LLDYHR
Sbjct: 99 NAIRLPFCTESVKPGTQPIG--IDYSKNPDLRGLDSLQIMEKIIKKAGDLGIFVLLDYHR 156
Query: 148 IGCEEIEPLWYTENYSEEQYIKDWIFLAKRFGKYPNVIGADIKNEPHG------------ 195
IGC IEPLWYTE++SEE +I WI +AKRFGKY NVIGAD+KNEPH
Sbjct: 157 IGCTHIEPLWYTEDFSEEDFINTWIEVAKRFGKYWNVIGADLKNEPHSVTSPPAAYTDGT 216
Query: 196 EAGWGTGD-ERDFRLFAEKVGREILKVAPHWLIFVEGTQYTHVPNIDEIIEKKGWWTFWG 254
A WG G+ D+ L AE++G+ ILKVAPHWLIFVEGTQ+T+ P D K G+ +WG
Sbjct: 217 GATWGMGNPATDWNLAAERIGKAILKVAPHWLIFVEGTQFTN-PKTDSSY-KWGYNAWWG 274
Query: 255 ENLMGVKDYPVRLPRGKVVYSPHVYGPSVYMMDYF-KSPDFPNNMPIIWETHFGYL-TDL 312
NLM VKDYPV LPR K+VYSPHVYGP VY YF + FP+N+P IW HFGY+ +L
Sbjct: 275 GNLMAVKDYPVNLPRNKLVYSPHVYGPDVYNQPYFGPAKGFPDNLPDIWYHHFGYVKLEL 334
Query: 313 NYTLVIGEWGGNY----EGLDKVWQDAFVKWLIKKKIYNFFYWCLNPESGDTGGIFLDDW 368
Y++VIGE+GG Y + D +WQ+ V W+I+ K +FFYW NP+SGDTGGI DDW
Sbjct: 335 GYSVVIGEFGGKYGHGGDPRDVIWQNKLVDWMIENKFCDFFYWSWNPDSGDTGGILQDDW 394
Query: 369 KTVNWEKMRVIYRLIKAANPEFEEPLYIILKTNATTS 405
T+ +K + RL+ + + +I T T S
Sbjct: 395 TTIWEDKYNNLKRLMDSCSKSSSSTQSVIRSTTPTKS 431
>pir||E82759 endo-1,4-beta-glucanase XF0818 [imported] - Xylella fastidiosa
(strain 9a5c) >gi|9105718|gb|AAF83628.1|AE003921_8
(AE003921) endo-1,4-beta-glucanase [Xylella fastidiosa]
Length = 592
Score = 321 bits (814), Expect = 1e-86
Identities = 157/361 (43%), Positives = 219/361 (60%), Gaps = 18/361 (4%)
Query: 26 PDVNYTAENGIIFVQNVTTGEKKPLYLHGVSWFGFELKDHVVYGLDKRNWKDILKDVKRL 85
P +Y+ +G V + + L G SWFGFE +HVV+GL RNWK+ + ++ +
Sbjct: 22 PVFSYSISHG-----KVIDDKGNQIQLKGGSWFGFETTNHVVHGLWTRNWKEFITQIQSM 76
Query: 86 GFNAIRLPFCSESIRPDTRPSPERINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDY 145
G NA+RLPFC S+ +T SP I+Y NPDL+ L+SL+IM+K+++ + G+Y+L+D+
Sbjct: 77 GLNAVRLPFCPASLNSNT--SPSSIDYNRNPDLQGLSSLQIMDKVVKELSDRGIYVLMDH 134
Query: 146 HRIGCEEIEPLWYTENYSEEQYIKDWIFLAKRFGKYPNVIGADIKNEPHGEAGWGTGDER 205
H C I LWYT++YSE+Q+I D F+A R+ P VIG D+KNEPHG A WGTGD +
Sbjct: 135 HTPDCAAISELWYTDSYSEKQWIDDLRFVAHRYANVPGVIGLDVKNEPHGRATWGTGDPK 194
Query: 206 -DFRLFAEKVGREILKVAPHWLIFVEGTQYTHVPNIDEIIEKKGWWTFWGENLMGVKDYP 264
D+ E IL+ AP WLI VEG P+ I FWGENL + P
Sbjct: 195 TDWNTAVEHAAAAILEAAPKWLIGVEGIGEN--PSCSSTIGH-----FWGENLEPMDCTP 247
Query: 265 VRLPRGKVVYSPHVYGPSVYMMDYFKSPDFPNNMPIIWETHFGYLTDLNYTLVIGEWGGN 324
+++P ++ PHVYGP VY+ YF SPDFPNNM IW+ HFG+ Y + IGE+GG
Sbjct: 248 LKVPADHLLLIPHVYGPDVYVQPYFNSPDFPNNMAAIWDKHFGHFAKAGYAMAIGEFGGK 307
Query: 325 Y---EGLDKVWQDAFVKWLIKKKIYNFFYWCLNPESGDTGGIFLDDWKTVNWEKMRVIYR 381
Y + D WQ+A V +LI + + FYW +N S DTGG+F DDW V +K+ ++ +
Sbjct: 308 YGEGDPRDVAWQNALVDYLISIGVTDTFYWSINANSSDTGGLFRDDWNHVREDKLTLLKK 367
Query: 382 L 382
L
Sbjct: 368 L 368
>sp|P19487|GUNA_XANCP MAJOR EXTRACELLULAR ENDOGLUCANASE PRECURSOR
(ENDO-1,4-BETA-GLUCANASE) (CELLULASE)
>gi|77831|pir||JH0158 cellulase (EC 3.2.1.4) precursor -
Xanthomonas campestris pv. campestris
>gi|155398|gb|AAA27612.1| (M32700) major extracellular
endoglucanase (engXCA) precursor [Xanthomonas
campestris]
Length = 493
Score = 315 bits (799), Expect = 7e-85
Identities = 159/361 (44%), Positives = 219/361 (60%), Gaps = 18/361 (4%)
Query: 26 PDVNYTAENGIIFVQNVTTGEKKPLYLHGVSWFGFELKDHVVYGLDKRNWKDILKDVKRL 85
P +Y+ N + + K + L GV+ FGFE +HV++GL RNWKD++ ++ L
Sbjct: 22 PAFSYSINNS----RQIVDDSGKVVQLKGVNVFGFETGNHVMHGLWARNWKDMIVQMQGL 77
Query: 86 GFNAIRLPFCSESIRPDTRPSPERINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDY 145
GFNA+RLPFC ++R DT P+ I+Y N DL+ LTSL+I++K+I N+ G+Y+LLD+
Sbjct: 78 GFNAVRLPFCPATLRSDTMPAS--IDYSRNADLQGLTSLQILDKVIAEFNARGMYVLLDH 135
Query: 146 HRIGCEEIEPLWYTENYSEEQYIKDWIFLAKRFGKYPNVIGADIKNEPHGEAGWGTGD-E 204
H C I LWYT +Y+E Q++ D F+A R+ P V+G D+KNEPHG A WGTG+
Sbjct: 136 HTPDCAGISELWYTGSYTEAQWLADLRFVANRYKNVPYVLGLDLKNEPHGAATWGTGNAA 195
Query: 205 RDFRLFAEKVGREILKVAPHWLIFVEGTQYTHVPNIDEIIEKKGWWTFWGENLMGVKDYP 264
D+ AE+ +L VAP WLI VEG D + FWG NL + P
Sbjct: 196 TDWNKAAERGSAAVLAVAPKWLIAVEGI-------TDNPVCSTNGGIFWGGNLQPLACTP 248
Query: 265 VRLPRGKVVYSPHVYGPSVYMMDYFKSPDFPNNMPIIWETHFGYLTDLNYTLVIGEWGGN 324
+ +P +++ +PHVYGP V++ YF +FPNNMP IWE HFG + L++GE+GG
Sbjct: 249 LNIPANRLLLAPHVYGPDVFVQSYFNDSNFPNNMPAIWERHFGQFAG-THALLLGEFGGK 307
Query: 325 Y---EGLDKVWQDAFVKWLIKKKIYNFFYWCLNPESGDTGGIFLDDWKTVNWEKMRVIYR 381
Y + DK WQDA VK+L K I FYW NP SGDTGGI DDW +V +KM ++
Sbjct: 308 YGEGDARDKTWQDALVKYLRSKGINQGFYWSWNPNSGDTGGILRDDWTSVRQDKMTLLRT 367
Query: 382 L 382
L
Sbjct: 368 L 368
>emb|CAC18529.1| (AJ304415) extracellular endoglucanase (ENGXCA protein)
[Xanthomonas campestris pv. campestris]
Length = 518
Score = 315 bits (799), Expect = 7e-85
Identities = 159/361 (44%), Positives = 219/361 (60%), Gaps = 18/361 (4%)
Query: 26 PDVNYTAENGIIFVQNVTTGEKKPLYLHGVSWFGFELKDHVVYGLDKRNWKDILKDVKRL 85
P +Y+ N + + K + L GV+ FGFE +HV++GL RNWKD++ ++ L
Sbjct: 22 PAFSYSINNS----RQIVDDSGKVVQLKGVNVFGFETGNHVMHGLWARNWKDMIVQMQGL 77
Query: 86 GFNAIRLPFCSESIRPDTRPSPERINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDY 145
GFNA+RLPFC ++R DT P+ I+Y N DL+ LTSL+I++K+I N+ G+Y+LLD+
Sbjct: 78 GFNAVRLPFCPATLRSDTMPAS--IDYSRNADLQGLTSLQILDKVIAEFNARGMYVLLDH 135
Query: 146 HRIGCEEIEPLWYTENYSEEQYIKDWIFLAKRFGKYPNVIGADIKNEPHGEAGWGTGD-E 204
H C I LWYT +Y+E Q++ D F+A R+ P V+G D+KNEPHG A WGTG+
Sbjct: 136 HTPDCAGISELWYTGSYTEAQWLADLRFVANRYKNVPYVLGLDLKNEPHGAATWGTGNAA 195
Query: 205 RDFRLFAEKVGREILKVAPHWLIFVEGTQYTHVPNIDEIIEKKGWWTFWGENLMGVKDYP 264
D+ AE+ +L VAP WLI VEG D + FWG NL + P
Sbjct: 196 TDWNKAAERGSAAVLAVAPKWLIAVEGI-------TDNPVCSTNGGIFWGGNLQPLACTP 248
Query: 265 VRLPRGKVVYSPHVYGPSVYMMDYFKSPDFPNNMPIIWETHFGYLTDLNYTLVIGEWGGN 324
+ +P +++ +PHVYGP V++ YF +FPNNMP IWE HFG + L++GE+GG
Sbjct: 249 LNIPANRLLLAPHVYGPDVFVQSYFNDSNFPNNMPAIWERHFGQFAG-THALLLGEFGGK 307
Query: 325 Y---EGLDKVWQDAFVKWLIKKKIYNFFYWCLNPESGDTGGIFLDDWKTVNWEKMRVIYR 381
Y + DK WQDA VK+L K I FYW NP SGDTGGI DDW +V +KM ++
Sbjct: 308 YGEGDARDKTWQDALVKYLRSKGINQGFYWSWNPNSGDTGGILRDDWTSVRQDKMTLLRT 367
Query: 382 L 382
L
Sbjct: 368 L 368
>sp|P23548|GUN_PAEPO ENDOGLUCANASE (ENDO-1,4-BETA-GLUCANASE) (CELLULASE)
>gi|143271|gb|AAA22631.1| (M33791)
endo-beta-1,4-glucanase [Paenibacillus polymyxa]
Length = 397
Score = 285 bits (721), Expect = 1e-75
Identities = 147/343 (42%), Positives = 207/343 (59%), Gaps = 17/343 (4%)
Query: 48 KPLYLHGVSWFGFELKDHVVYGLDKRNWKDILKDVKRLGFNAIRLPFCSESIRPDTRPSP 107
K +G++WFG E ++ ++GL R+ D+L VK+ G+N IRLP+ ++ D+ P
Sbjct: 52 KEAAFNGLNWFGLETPNYTLHGLWSRSMDDMLDQVKKEGYNLIRLPYSNQLF--DSSSRP 109
Query: 108 ERINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDYHRIGCEEIEPLWYTENYSEEQY 167
+ I+Y NPDL L ++IM+K+IE A G+ I+LD HR G LWYT Y E ++
Sbjct: 110 DSIDYHKNPDLVGLNPIQIMDKLIEKAGQRGIQIILDRHRPGSGGQSELWYTSQYPESRW 169
Query: 168 IKDWIFLAKRFGKYPNVIGADIKNEPHGEAGWGTGD-ERDFRLFAEKVGREILKVAPHWL 226
I DW LA R+ P VIGAD+ NEPHG+A WGTG+ D+RL A++ G IL V P+WL
Sbjct: 170 ISDWKMLADRYKNNPTVIGADLHNEPHGQASWGTGNASTDWRLAAQRAGNAILSVNPNWL 229
Query: 227 IFVEGTQYTHVPNIDEIIEKKGWWTFWGENLMGVKDYPVRLP-RGKVVYSPHVYGPSVYM 285
I VEG +D ++ +WG NL GV +YPV L +VVYSPH YGP V
Sbjct: 230 ILVEG--------VDHNVQGNNSQYWWGGNLTGVANYPVVLDVPNRVVYSPHDYGPGVSS 281
Query: 286 MDYFKSPDFPNNMPIIWETHFGYLTDLNYT-LVIGEWGGNYEGL---DKVWQDAFVKWLI 341
+F P FP+N+P IW+ +GY++ N +++GE+GG L + WQ+A V ++
Sbjct: 282 QPWFNDPAFPSNLPAIWDQTWGYISKQNIAPVLVGEFGGRNVDLSCPEGKWQNALVHYIG 341
Query: 342 KKKIYNFFYWCLNPESGDTGGIFLDDWKTVNWEKMRVIYRLIK 384
+Y F YW LNP SGDTGG+ LDDW T N K ++ R++K
Sbjct: 342 ANNLY-FTYWSLNPNSGDTGGLLLDDWTTWNRPKQDMLGRIMK 383
>pir||A35136 cellulase (EC 3.2.1.4) - Bacillus polymyxa
Length = 397
Score = 285 bits (721), Expect = 1e-75
Identities = 147/343 (42%), Positives = 207/343 (59%), Gaps = 17/343 (4%)
Query: 48 KPLYLHGVSWFGFELKDHVVYGLDKRNWKDILKDVKRLGFNAIRLPFCSESIRPDTRPSP 107
K +G++WFG E ++ ++GL R+ D+L VK+ G+N IRLP+ ++ D+ P
Sbjct: 52 KEAAFNGLNWFGLETPNYTLHGLWSRSMDDMLDQVKKEGYNLIRLPYSNQLF--DSSSRP 109
Query: 108 ERINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDYHRIGCEEIEPLWYTENYSEEQY 167
+ I+Y NPDL L ++IM+K+IE A G+ I+LD HR G LWYT Y E ++
Sbjct: 110 DSIDYHKNPDLVGLNPIQIMDKLIEKAGQRGIQIILDRHRPGSGGQSELWYTSQYPESRW 169
Query: 168 IKDWIFLAKRFGKYPNVIGADIKNEPHGEAGWGTGD-ERDFRLFAEKVGREILKVAPHWL 226
I DW LA R+ P VIGAD+ NEPHG+A WGTG+ D+RL A++ G IL V P+WL
Sbjct: 170 ISDWKMLADRYKNNPTVIGADLHNEPHGQASWGTGNASTDWRLAAQRAGNAILSVNPNWL 229
Query: 227 IFVEGTQYTHVPNIDEIIEKKGWWTFWGENLMGVKDYPVRLP-RGKVVYSPHVYGPSVYM 285
I VEG +D ++ +WG NL GV +YPV L +VVYSPH YGP V
Sbjct: 230 ILVEG--------VDHNVQGNNSQYWWGGNLTGVANYPVVLDVPNRVVYSPHDYGPGVSS 281
Query: 286 MDYFKSPDFPNNMPIIWETHFGYLTDLNYT-LVIGEWGGNYEGL---DKVWQDAFVKWLI 341
+F P FP+N+P IW+ +GY++ N +++GE+GG L + WQ+A V ++
Sbjct: 282 QPWFNDPAFPSNLPAIWDQTWGYISKQNIAPVLVGEFGGRNVDLSSPEGKWQNALVHYIG 341
Query: 342 KKKIYNFFYWCLNPESGDTGGIFLDDWKTVNWEKMRVIYRLIK 384
+Y F YW LNP SGDTGG+ LDDW T N K ++ R++K
Sbjct: 342 ANNLY-FTYWSLNPNSGDTGGLLLDDWTTWNRPKQDMLGRIMK 383
>sp|P54583|GUN1_ACICE ENDOGLUCANASE E1 PRECURSOR (ENDO-1,4-BETA-GLUCANASE E1) (CELLULASE
E1) (ENDOCELLULASE E1) >gi|988300|gb|AAA75477.1|
(U33212) E I beta-1,4-endoglucanase precursor
[Acidothermus cellulolyticus]
Length = 562
Score = 264 bits (668), Expect = 2e-69
Identities = 143/336 (42%), Positives = 197/336 (58%), Gaps = 21/336 (6%)
Query: 49 PLYLHGVSWFGFELKDHVVYGLDKRNWKDILKDVKRLGFNAIRLPFCSESIRPDTRPSPE 108
P+ + G++WFGFE ++VV+GL R+++ +L +K LG+N IRLP+ + ++P T P+
Sbjct: 61 PVRIAGINWFGFETCNYVVHGLWSRDYRSMLDQIKSLGYNTIRLPYSDDILKPGTMPNSI 120
Query: 109 RINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDYHRIGCEEIEPLWYTENYSEEQYI 168
Y++N DL+ LTSL++M+KI+ YA IGL I+LD HR C LWYT + SE +I
Sbjct: 121 NF-YQMNQDLQGLTSLQVMDKIVAYAGQIGLRIILDRHRPDCSGQSALWYTSSVSEATWI 179
Query: 169 KDWIFLAKRFGKYPNVIGADIKNEPHGEAGWGTGDER-DFRLFAEKVGREILKVAPHWLI 227
D LA+R+ P V+G D+ NEPH A WG GD D+RL AE+ G +L V P+ LI
Sbjct: 180 SDLQALAQRYKGNPTVVGFDLHNEPHDPACWGCGDPSIDWRLAAERAGNAVLSVNPNLLI 239
Query: 228 FVEGTQYTHVPNIDEIIEKKGWWTFWGENLMGVKDYPVRL-PRGKVVYSPHVYGPSVYMM 286
FVEG Q G +WG NL G YPV L ++VYS H Y SVY
Sbjct: 240 FVEGVQ-----------SYNGDSYWWGGNLQGAGQYPVVLNVPNRLVYSAHDYATSVYPQ 288
Query: 287 DYFKSPDFPNNMPIIWETHFGYLTDLNYTLV-IGEWGGNYEG-LDKVWQDAFVKWLIKKK 344
+F P FPNNMP IW ++GYL + N V +GE+G + D+ W V++L
Sbjct: 289 TWFSDPTFPNNMPGIWNKNWGYLFNQNIAPVWLGEFGTTLQSTTDQTWLKTLVQYLRPTA 348
Query: 345 IY-----NFFYWCLNPESGDTGGIFLDDWKTVNWEK 375
Y + +W NP+SGDTGGI DDW+TV+ K
Sbjct: 349 QYGADSFQWTFWSWNPDSGDTGGILKDDWQTVDTVK 384
>pdb|1ECE|A Chain A, Acidothermus Cellulolyticus Endocellulase E1 Catalytic
Domain In Complex With A Cellotetraose
>gi|1827682|pdb|1ECE|B Chain B, Acidothermus
Cellulolyticus Endocellulase E1 Catalytic Domain In
Complex With A Cellotetraose
Length = 358
Score = 264 bits (668), Expect = 2e-69
Identities = 143/336 (42%), Positives = 197/336 (58%), Gaps = 21/336 (6%)
Query: 49 PLYLHGVSWFGFELKDHVVYGLDKRNWKDILKDVKRLGFNAIRLPFCSESIRPDTRPSPE 108
P+ + G++WFGFE ++VV+GL R+++ +L +K LG+N IRLP+ + ++P T P+
Sbjct: 20 PVRIAGINWFGFETCNYVVHGLWSRDYRSMLDQIKSLGYNTIRLPYSDDILKPGTMPNSI 79
Query: 109 RINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDYHRIGCEEIEPLWYTENYSEEQYI 168
Y++N DL+ LTSL++M+KI+ YA IGL I+LD HR C LWYT + SE +I
Sbjct: 80 NF-YQMNQDLQGLTSLQVMDKIVAYAGQIGLRIILDRHRPDCSGQSALWYTSSVSEATWI 138
Query: 169 KDWIFLAKRFGKYPNVIGADIKNEPHGEAGWGTGDER-DFRLFAEKVGREILKVAPHWLI 227
D LA+R+ P V+G D+ NEPH A WG GD D+RL AE+ G +L V P+ LI
Sbjct: 139 SDLQALAQRYKGNPTVVGFDLHNEPHDPACWGCGDPSIDWRLAAERAGNAVLSVNPNLLI 198
Query: 228 FVEGTQYTHVPNIDEIIEKKGWWTFWGENLMGVKDYPVRL-PRGKVVYSPHVYGPSVYMM 286
FVEG Q G +WG NL G YPV L ++VYS H Y SVY
Sbjct: 199 FVEGVQ-----------SYNGDSYWWGGNLQGAGQYPVVLNVPNRLVYSAHDYATSVYPQ 247
Query: 287 DYFKSPDFPNNMPIIWETHFGYLTDLNYTLV-IGEWGGNYEG-LDKVWQDAFVKWLIKKK 344
+F P FPNNMP IW ++GYL + N V +GE+G + D+ W V++L
Sbjct: 248 TWFSDPTFPNNMPGIWNKNWGYLFNQNIAPVWLGEFGTTLQSTTDQTWLKTLVQYLRPTA 307
Query: 345 IY-----NFFYWCLNPESGDTGGIFLDDWKTVNWEK 375
Y + +W NP+SGDTGGI DDW+TV+ K
Sbjct: 308 QYGADSFQWTFWSWNPDSGDTGGILKDDWQTVDTVK 343
>pdb|1C0D|A Chain A, Endocellulase E1 From Acidothermus Cellulolyticus Mutant
Y245g >gi|5821930|pdb|1C0D|B Chain B, Endocellulase E1
From Acidothermus Cellulolyticus Mutant Y245g
Length = 358
Score = 262 bits (662), Expect = 8e-69
Identities = 142/336 (42%), Positives = 197/336 (58%), Gaps = 21/336 (6%)
Query: 49 PLYLHGVSWFGFELKDHVVYGLDKRNWKDILKDVKRLGFNAIRLPFCSESIRPDTRPSPE 108
P+ + G++WFGFE ++VV+GL R+++ +L +K LG+N IRLP+ + ++P T P+
Sbjct: 20 PVRIAGINWFGFETCNYVVHGLWSRDYRSMLDQIKSLGYNTIRLPYSDDILKPGTMPNSI 79
Query: 109 RINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDYHRIGCEEIEPLWYTENYSEEQYI 168
Y++N DL+ LTSL++M+KI+ YA IGL I+LD HR C LWYT + SE +I
Sbjct: 80 NF-YQMNQDLQGLTSLQVMDKIVAYAGQIGLRIILDRHRPDCSGQSALWYTSSVSEATWI 138
Query: 169 KDWIFLAKRFGKYPNVIGADIKNEPHGEAGWGTGDER-DFRLFAEKVGREILKVAPHWLI 227
D LA+R+ P V+G D+ NEPH A WG GD D+RL AE+ G +L V P+ LI
Sbjct: 139 SDLQALAQRYKGNPTVVGFDLHNEPHDPACWGCGDPSIDWRLAAERAGNAVLSVNPNLLI 198
Query: 228 FVEGTQYTHVPNIDEIIEKKGWWTFWGENLMGVKDYPVRL-PRGKVVYSPHVYGPSVYMM 286
FVEG Q G +WG NL G YPV L ++VYS H Y SV
Sbjct: 199 FVEGVQ-----------SYNGDSYWWGGNLQGAGQYPVVLNVPNRLVYSAHDYATSVGPQ 247
Query: 287 DYFKSPDFPNNMPIIWETHFGYLTDLNYTLV-IGEWGGNYEG-LDKVWQDAFVKWLIKKK 344
+F P FPNNMP IW ++GYL + N V +GE+G + D+ W V++L
Sbjct: 248 TWFSDPTFPNNMPGIWNKNWGYLFNQNIAPVWLGEFGTTLQSTTDQTWLKTLVQYLRPTA 307
Query: 345 IY-----NFFYWCLNPESGDTGGIFLDDWKTVNWEK 375
Y + +W NP+SGDTGGI DDW+TV+ +K
Sbjct: 308 QYGADSFQWTFWSWNPDSGDTGGILKDDWQTVDTDK 343
>emb|CAA44467.2| (X62582) Cellulase [Clavibacter michiganensis]
Length = 745
Score = 224 bits (565), Expect = 2e-57
Identities = 131/361 (36%), Positives = 187/361 (51%), Gaps = 26/361 (7%)
Query: 20 VIALSYPDVNYTAENGIIFVQNVTTGEKKPLYLHGVSWFGFELKDHVVYGLDKRNWKDIL 79
V A S P +TA I+ T P + G++WFG E +GLD +
Sbjct: 63 VAAASSPGWLHTAGGKIV------TASGAPYTIRGIAWFGMETSSCAPHGLDTITLAGGM 116
Query: 80 KDVKRLGFNAIRLPFCSESIRPDTRPSPERINYELNPDLKNLTSLEIMEKIIEYANSIGL 139
+ +K++GF +RLPF ++ + + +P L LT L++M+ ++ A S GL
Sbjct: 117 QHIKQMGFTTVRLPFSNQCLA-----ASGVTGVSADPSLAGLTPLQVMDHVVASAKSAGL 171
Query: 140 YILLDYHRIGCEEIEPLWYTENYSEEQYIKDWIFLAKRFGKYPNVIGADIKNEPHGEAGW 199
++LD HR LWYT Y E ++I DW LAKR+ P VIG D+ NEPHG A W
Sbjct: 172 DVILDQHRPDSGGQSELWYTSQYPESRWISDWRMLAKRYAAEPTVIGVDLHNEPHGAATW 231
Query: 200 GTG-DERDFRLFAEKVGREILKVAPHWLIFVEGTQYTHVPNIDEIIEKKGWWTFWGENLM 258
GTG D+R AE+ G +L P+ L+ VEG + E G T+WG L
Sbjct: 232 GTGAATTDWRAAAERGGNAVLAENPNLLVLVEGIDH----------EADGSGTWWGGALG 281
Query: 259 GVKDYPVRLP-RGKVVYSPHVYGPSVYMMDYFKSPDFPNNMPIIWETHFGYLTDLNYT-L 316
V + PVRL +VVYSPH Y ++Y +F + ++P N+P IW+ H+GYL + +
Sbjct: 282 LVGNAPVRLSVANRVVYSPHDYPSTIYGQSWFSASNYPANLPGIWDAHWGYLAKKDIAPV 341
Query: 317 VIGEWGGNYEGL-DKVWQDAFVKWLIKKKIYNFFYWCLNPESGDTGGIFLDDWKTVNWEK 375
++GE+G +E DK W + V +L I + F W NP SGDTGGI DW T K
Sbjct: 342 LVGEFGTKFETTSDKQWLNTLVGYLSSTGISSSF-WAFNPNSGDTGGIVKSDWVTPEQAK 400
Query: 376 M 376
+
Sbjct: 401 L 401
>sp|P10474|GUNB_CALSA ENDOGLUCANASE/EXOGLUCANASE B PRECURSOR [INCLUDES: ENDOGLUCANASE
(ENDO-1,4-BETA-GLUCANASE) (CELLULASE)
(CELLOBIOHYDROLASE); EXOGLUCANASE (EXOCELLOBIOHYDROLASE)
(1,4-BETA-CELLOBIOHYDROLASE)] >gi|77446|pir||S02711
cellulase (EC 3.2.1.4) precursor - Caldocellum
saccharolyticum >gi|40646|emb|CAA31936.1| (X13602) celB
polypeptide precursor [Caldicellulosiruptor
saccharolyticus]
Length = 1039
Score = 203 bits (512), Expect = 3e-51
Identities = 130/397 (32%), Positives = 205/397 (50%), Gaps = 33/397 (8%)
Query: 48 KPLYLHGVSWFGFELKDHVVYGLDKRNWKDILKDVKRLGFNAIRLPFCSESIRPDTR--- 104
+P++L G++WFG+ +V G+ N KD L ++ GFN +R+P +E I ++
Sbjct: 643 RPVWLTGINWFGYNTGTNVFDGVWSCNLKDTLAEIANRGFNLLRVPISAELILNWSQGIY 702
Query: 105 PSPERINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDYHRIGCE---EIEPLWYTEN 161
P P INY +NP+L+ SLE+ + +++ +GL I+LD H I + I P+WY E
Sbjct: 703 PKPN-INYYVNPELEGKNSLEVFDIVVQTCKEVGLKIMLDIHSIKTDAMGHIYPVWYDEK 761
Query: 162 YSEEQYIKDWIFLAKRFGKYPNVIGADIKNEPHGE-------AGWGTG-DERDFRLFAEK 213
++ E + K ++ R+ +I D+KNEPHG+ A W D +++ AE
Sbjct: 762 FTPEDFYKACEWITNRYKNDDTIIAFDLKNEPHGKPWQDTTFAKWDNSTDINNWKYAAET 821
Query: 214 VGREILKVAPHWLIFVEGTQYTHVPNIDEIIEKKG----WWTFWGENLMGVKDYPVRLPR 269
+ IL + P+ LI +EG + P D K + T+WG NL GV+ YP+ L +
Sbjct: 822 CAKRILNINPNLLIVIEGIEA--YPKDDVTWTSKSSSDYYSTWWGGNLRGVRKYPINLGK 879
Query: 270 --GKVVYSPHVYGPSVYMMDYFKSPDFPNNMPI--IWETHFGYLTDLNYT-LVIGEWGGN 324
KVVYSPH YGPSVY +F P F + W ++ Y+ + N L+IGEWGG+
Sbjct: 880 YQNKVVYSPHDYGPSVYQQPWFY-PGFTKESLLQDCWRPNWAYIMEENIAPLLIGEWGGH 938
Query: 325 YEGLD-KVWQDAFVKWLIKKKIYNFFYWCLNPESGDTGGIFLDDWKTVNWEKMRVIYRLI 383
+G D + W ++I+ I++ F WC N SGDTGG+ D+ T W++ + Y +
Sbjct: 939 LDGADNEKWMKYLRDYIIENHIHHTF-WCFNANSGDTGGLVGYDFTT--WDEKK--YSFL 993
Query: 384 KAANPEFEEPLYIILKTNATTSILGVGERIRIYWYTN 420
K A + + ++ L G I Y+ N
Sbjct: 994 KPALWQDSQGRFVGLDHKRPLGTNGKNINITTYYNNN 1030
>pir||A43802 cellulase (EC 3.2.1.4) / cellulose 1,4-beta-cellobiosidase (EC
3.2.1.91) - Caldocellum saccharolyticum (fragments)
Length = 915
Score = 203 bits (512), Expect = 3e-51
Identities = 130/397 (32%), Positives = 205/397 (50%), Gaps = 33/397 (8%)
Query: 48 KPLYLHGVSWFGFELKDHVVYGLDKRNWKDILKDVKRLGFNAIRLPFCSESIRPDTR--- 104
+P++L G++WFG+ +V G+ N KD L ++ GFN +R+P +E I ++
Sbjct: 519 RPVWLTGINWFGYNTGTNVFDGVWSCNLKDTLAEIANRGFNLLRVPISAELILNWSQGIY 578
Query: 105 PSPERINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDYHRIGCE---EIEPLWYTEN 161
P P INY +NP+L+ SLE+ + +++ +GL I+LD H I + I P+WY E
Sbjct: 579 PKPN-INYYVNPELEGKNSLEVFDIVVQTCKEVGLKIMLDIHSIKTDAMGHIYPVWYDEK 637
Query: 162 YSEEQYIKDWIFLAKRFGKYPNVIGADIKNEPHGE-------AGWGTG-DERDFRLFAEK 213
++ E + K ++ R+ +I D+KNEPHG+ A W D +++ AE
Sbjct: 638 FTPEDFYKACEWITNRYKNDDTIIAFDLKNEPHGKPWQDTTFAKWDNSTDINNWKYAAET 697
Query: 214 VGREILKVAPHWLIFVEGTQYTHVPNIDEIIEKKG----WWTFWGENLMGVKDYPVRLPR 269
+ IL + P+ LI +EG + P D K + T+WG NL GV+ YP+ L +
Sbjct: 698 CAKRILNINPNLLIVIEGIEA--YPKDDVTWTSKSSSDYYSTWWGGNLRGVRKYPINLGK 755
Query: 270 --GKVVYSPHVYGPSVYMMDYFKSPDFPNNMPI--IWETHFGYLTDLNYT-LVIGEWGGN 324
KVVYSPH YGPSVY +F P F + W ++ Y+ + N L+IGEWGG+
Sbjct: 756 YQNKVVYSPHDYGPSVYQQPWFY-PGFTKESLLQDCWRPNWAYIMEENIAPLLIGEWGGH 814
Query: 325 YEGLD-KVWQDAFVKWLIKKKIYNFFYWCLNPESGDTGGIFLDDWKTVNWEKMRVIYRLI 383
+G D + W ++I+ I++ F WC N SGDTGG+ D+ T W++ + Y +
Sbjct: 815 LDGADNEKWMKYLRDYIIENHIHHTF-WCFNANSGDTGGLVGYDFTT--WDEKK--YSFL 869
Query: 384 KAANPEFEEPLYIILKTNATTSILGVGERIRIYWYTN 420
K A + + ++ L G I Y+ N
Sbjct: 870 KPALWQDSQGRFVGLDHKRPLGTNGKNINITTYYNNN 906
>gb|AAD30364.1|AF078739_2 (AF078739) CelB [Caldicellulosiruptor sp. Tok7B.1]
Length = 1426
Score = 203 bits (512), Expect = 3e-51
Identities = 140/423 (33%), Positives = 214/423 (50%), Gaps = 36/423 (8%)
Query: 26 PDVNYTAENGIIFVQN--VTTGEKKPLYLHGVSWFGFELKDHVVYGLDKRNWKDILKDVK 83
P V + +F Q + + KP++L GV+WFGF +V G+ N K L ++
Sbjct: 1007 PSVTDDTNDDWLFAQGNKIVDKDGKPVWLTGVNWFGFNTGTNVFDGVWSCNLKSALAEIA 1066
Query: 84 RLGFNAIRLPFCSESIRPDTR---PSPERINYELNPDLKNLTSLEIMEKIIEYANSIGLY 140
GFN +R+P +E I ++ P P INY +NP+L+ LTSLE+ + +++ +GL
Sbjct: 1067 NRGFNLLRVPISAELILNWSKGIYPKP-NINYYVNPELEGLTSLEVFDFVVKTCKEVGLK 1125
Query: 141 ILLDYHRIGCE---EIEPLWYTENYSEEQYIKDWIFLAKRFGKYPNVIGADIKNEPHGE- 196
I+LD H + I P+WYT+ + E Y K ++ +R+ ++ D+KNEPHG+
Sbjct: 1126 IMLDIHSAKTDAMGHIYPVWYTDTITPEDYYKACEWITERYKNDDTIVAFDLKNEPHGKP 1185
Query: 197 ------AGW-GTGDERDFRLFAEKVGREILKVAPHWLIFVEGTQYTHVPNIDEIIEKKG- 248
A W + D +++ AE + IL P+ LI +EG + P D K
Sbjct: 1186 WQDSVFAKWDNSTDINNWKYAAETCAKRILAKNPNMLIVIEGIE--AYPKDDVTWTSKSS 1243
Query: 249 ---WWTFWGENLMGVKDYPVRLP--RGKVVYSPHVYGPSVYMMDYFKSPDFPNNMPI--I 301
+ T+WG NL GVK YP+ L + KVVYSPH YGP VY +F P F +
Sbjct: 1244 SDYYSTWWGGNLRGVKKYPINLGQYQNKVVYSPHDYGPLVYQQPWF-YPGFTKDTLYNDC 1302
Query: 302 WETHFGYLTDLNYT-LVIGEWGGNYEGLD-KVWQDAFVKWLIKKKIYNFFYWCLNPESGD 359
W ++ Y+ D L+IGEWGG +G D + W ++I+ I++ F WC N SGD
Sbjct: 1303 WRDNWTYIMDNGIAPLLIGEWGGYLDGGDNEKWMTYLRDYIIENHIHHTF-WCYNANSGD 1361
Query: 360 TGGIFLDDWKTVNWEKMRVIYRLIKAANPEFEEPLYIILKTNATTSILGVGERIRIYWYT 419
TGG+ D+ T W++ + Y +K A + + ++ L G I IY Y
Sbjct: 1362 TGGLVGYDFST--WDEQK--YNFLKPALWQDSKGRFVGLDHKRPLGTNGKNINITIY-YQ 1416
Query: 420 NGK 422
NG+
Sbjct: 1417 NGE 1419
>dbj|BAA22939.1| (AB008029) beta-glucanase [thermophilic anaerobe NA10]
Length = 1000
Score = 201 bits (507), Expect = 1e-50
Identities = 130/397 (32%), Positives = 204/397 (50%), Gaps = 33/397 (8%)
Query: 48 KPLYLHGVSWFGFELKDHVVYGLDKRNWKDILKDVKRLGFNAIRLPFCSESIRPDTR--- 104
+P++L GV+WFG+ +V G+ N K L ++ GFN +R+P +E I ++
Sbjct: 604 RPVWLTGVNWFGYNTGTNVFDGVWSCNLKSTLAEIANRGFNLLRVPISAELILNWSQGIY 663
Query: 105 PSPERINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDYHRIGCE---EIEPLWYTEN 161
P P INY +NP+L+ SLE+ + +++ +GL I+LD H I + I P+WY E
Sbjct: 664 PKPN-INYYVNPELEGKNSLEVFDIVVQTCKEVGLKIMLDIHSIKTDAMGHIYPVWYDEK 722
Query: 162 YSEEQYIKDWIFLAKRFGKYPNVIGADIKNEPHGE-------AGWGTG-DERDFRLFAEK 213
++ E + K ++ R+ +I D+KNEPHG+ A W D +++ AE
Sbjct: 723 FTPEDFYKACEWITNRYKNDDTIIAFDLKNEPHGKPWQDTTFAKWDNSTDINNWKYAAET 782
Query: 214 VGREILKVAPHWLIFVEGTQYTHVPNIDEIIEKKG----WWTFWGENLMGVKDYPVRLPR 269
+ IL + P+ LI +EG + P D K + T+WG NL GV+ YP+ L +
Sbjct: 783 CAKRILNINPNLLIVIEGIEA--YPKDDVTWTSKSSSDYYSTWWGGNLRGVRKYPINLGK 840
Query: 270 --GKVVYSPHVYGPSVYMMDYFKSPDFPNNMPI--IWETHFGYLTDLNYT-LVIGEWGGN 324
KVVYSPH YGPSVY +F P F + W ++ Y+ + N L+IGEWGG+
Sbjct: 841 YQNKVVYSPHDYGPSVYQQPWFY-PGFTKESLLQDCWRPNWAYIMEENIAPLLIGEWGGH 899
Query: 325 YEGLD-KVWQDAFVKWLIKKKIYNFFYWCLNPESGDTGGIFLDDWKTVNWEKMRVIYRLI 383
+G D + W ++I+ I++ F WC N SGDTGG+ D+ T W++ + Y +
Sbjct: 900 LDGADNEKWMKYLRDYIIENHIHHTF-WCFNANSGDTGGLVGYDFTT--WDEKK--YSFL 954
Query: 384 KAANPEFEEPLYIILKTNATTSILGVGERIRIYWYTN 420
K A + + ++ L G I Y+ N
Sbjct: 955 KPALWQDSQGRFVGLDHKRPLGTNGKNINITTYYNNN 991
>emb|CAB76938.1| (AJ275975) 1,4-beta-cellobiohydrolase [Clostridium thermocellum]
Length = 660
Score = 201 bits (505), Expect = 2e-50
Identities = 123/349 (35%), Positives = 192/349 (54%), Gaps = 21/349 (6%)
Query: 50 LYLHGVSWFGFELKD-HVVYGLDKRNWKDILKDVKRLGFNAIRLPFCSESI---RPDTRP 105
+YL G++WFGFE + +GL+K N +D L + +LGFN +R+P +E I + R
Sbjct: 217 VYLTGINWFGFETDGANGFHGLNKCNLEDSLDLMAKLGFNILRIPISAEIILQWKNGERV 276
Query: 106 SPERINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDYHRIGCEEI-EPLWYTENYSE 164
+N NP L L+SLEI++ I + G+ ++D H + E LWY ++ +
Sbjct: 277 ETSFVNTYENPRLDGLSSLEILDYTINHMKKNGMKAMIDMHSSTKDSYQENLWYNKDITM 336
Query: 165 EQYIKDWIFLAKRFGKYPNVIGADIKNEPHGE------AGWG-TGDERDFRLFAEKVGRE 217
E++I+ W ++ +R+ VI D+KNEPHG+ A W + D +++ AE + E
Sbjct: 337 EEFIEAWKWIVERYKDDDTVIAVDLKNEPHGKYSGPNIAKWDDSNDPNNWKRAAEIIAEE 396
Query: 218 ILKVAPHWLIFVEGTQYTHVPNID--EIIEKKGWWTFWGENLMGVKDYPVRLPR-GKVVY 274
IL + P+ LI VEG + + D E + +WG NL GV D+PV + K+VY
Sbjct: 397 ILAINPNLLIVVEGVEAYPMEGYDYTNCGEFTTYCNWWGGNLRGVADHPVVISAPDKLVY 456
Query: 275 SPHVYGPSVYMMDYFKSPDFPNNMPI--IWETHFGYLTDLNYT-LVIGEWGGNY-EGLDK 330
S H YGP +YM +FK DF N W ++ Y+ + N ++IGEWGG ++
Sbjct: 457 SVHDYGPDIYMQPWFKK-DFDINTLYEECWYPNWYYIVEQNIAPMLIGEWGGKLINENNR 515
Query: 331 VWQDAFVKWLIKKKIYNFFYWCLNPESGDTGGIFLDDWKTVNWEKMRVI 379
W + ++ +KK+++ F W NP S DTGG+ L+DWKTV+ EK +I
Sbjct: 516 KWLECLATFIAEKKLHHTF-WAFNPNSADTGGLMLEDWKTVDEEKYAII 563
>sp|P50400|GUND_CELFI ENDOGLUCANASE D PRECURSOR (ENDO-1,4-BETA-GLUCANASE) (CELLULASE)
>gi|144423|gb|AAA23089.1| (L02544)
endo-1,4-beta-D-glucanase [Cellulomonas fimi]
Length = 747
Score = 192 bits (484), Expect = 6e-48
Identities = 117/353 (33%), Positives = 186/353 (52%), Gaps = 29/353 (8%)
Query: 48 KPLYLHGVSWFGFELKDHVVYGLDKRNWKDILKDVKRLGFNAIRLPFCSESI---RPDTR 104
K L GV+WFGF + V +GL N I + + + G N +R+P ++ + + T
Sbjct: 59 KEAILSGVNWFGFNASERVFHGLWSGNITQITQQMAQRGINVVRVPVSTQLLLEWKAGTF 118
Query: 105 PSPERINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDYHRIGCEE---IEPLWYTEN 161
P +N NP+L+ SL+I E + G+ + LD H + + +W+ +
Sbjct: 119 LKPN-VNTYANPELEGKNSLQIFEYWLTLCQKYGIKVFLDVHSAEADNSGHVYNMWWKGD 177
Query: 162 YSEEQYIKDWIFLAKRFGKYPNVIGADIKNEPHGEAG------W-GTGDERDFRLFAEKV 214
+ E + W + A R+ ++GADIKNEPHG G W GT D+ +F+ FAE
Sbjct: 178 ITTEDVYEGWEWAATRWKDDDTIVGADIKNEPHGTQGSTERAKWDGTTDKDNFKHFAETA 237
Query: 215 GREILKVAPHWLIFVEGTQYTHVPNID----EIIEKKGWWTFWGENLMGVKDYPVRLP-- 268
++IL + P+WL+FVEG + P + + + G W WG NL GV+D+P+ L
Sbjct: 238 SKKILAINPNWLVFVEGVEIYPKPGVPWTSTGLTDYYGTW--WGGNLRGVRDHPIDLGAH 295
Query: 269 RGKVVYSPHVYGPSVYMMDYF-KSPDFPNNMPIIWETHFGYLTDLNYT-LVIGEWGGNY- 325
+ ++VYSPH YGP V+ +F K D + +W ++ ++ D + L+IGEWGG
Sbjct: 296 QDQLVYSPHDYGPLVFDQKWFQKDFDKASLTADVWGPNWLFIHDEDIAPLLIGEWGGRLG 355
Query: 326 -EGLDKVWQDAFVKWLIKKKIYNFFYWCLNPESGDTGGIFLDDWKTVNWEKMR 377
+ W A + ++++ F W LNP SGDTGG+ LDDWKT W++++
Sbjct: 356 QDPRQDKWMAALRDLVAERRLSQTF-WVLNPNSGDTGGLLLDDWKT--WDEVK 405
>pir||B47093 cellulase (EC 3.2.1.4) CenD - Cellulomonas fimi
Length = 747
Score = 192 bits (484), Expect = 6e-48
Identities = 117/353 (33%), Positives = 186/353 (52%), Gaps = 29/353 (8%)
Query: 48 KPLYLHGVSWFGFELKDHVVYGLDKRNWKDILKDVKRLGFNAIRLPFCSESI---RPDTR 104
K L GV+WFGF + V +GL N I + + + G N +R+P ++ + + T
Sbjct: 59 KEAILSGVNWFGFNASERVFHGLWSGNITQITQQMAQRGINVVRVPVSTQLLLEWKAGTF 118
Query: 105 PSPERINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDYHRIGCEE---IEPLWYTEN 161
P +N NP+L+ SL+I E + G+ + LD H + + +W+ +
Sbjct: 119 LKPN-VNTYANPELEGKNSLQIFEYWLTLCQKYGIKVFLDVHSAEADNSGHVYNMWWKGD 177
Query: 162 YSEEQYIKDWIFLAKRFGKYPNVIGADIKNEPHGEAG------W-GTGDERDFRLFAEKV 214
+ E + W + A R+ ++GADIKNEPHG G W GT D+ +F+ FAE
Sbjct: 178 ITTEDVYEGWEWAATRWKDDDTIVGADIKNEPHGTQGSTERAKWDGTTDKDNFKHFAETA 237
Query: 215 GREILKVAPHWLIFVEGTQYTHVPNID----EIIEKKGWWTFWGENLMGVKDYPVRLP-- 268
++IL + P+WL+FVEG + P + + + G W WG NL GV+D+P+ L
Sbjct: 238 SKKILAINPNWLVFVEGVEIYPKPGVPWTSTGLTDYYGTW--WGGNLRGVRDHPIDLGAH 295
Query: 269 RGKVVYSPHVYGPSVYMMDYF-KSPDFPNNMPIIWETHFGYLTDLNYT-LVIGEWGGNY- 325
+ ++VYSPH YGP V+ +F K D + +W ++ ++ D + L+IGEWGG
Sbjct: 296 QDQLVYSPHDYGPLVFDQKWFQKDFDKASLTADVWGPNWLFIHDEDIAPLLIGEWGGRLG 355
Query: 326 -EGLDKVWQDAFVKWLIKKKIYNFFYWCLNPESGDTGGIFLDDWKTVNWEKMR 377
+ W A + ++++ F W LNP SGDTGG+ LDDWKT W++++
Sbjct: 356 QDPRQDKWMAALRDLVAERRLSQTF-WVLNPNSGDTGGLLLDDWKT--WDEVK 405
>sp|Q05332|GUNG_CLOTM ENDOGLUCANASE G PRECURSOR (EGG) (ENDO-1,4-BETA-GLUCANASE)
(CELLULASE G) >gi|538956|pir||A40589 cellulase (EC
3.2.1.4) - Clostridium thermocellum
>gi|40678|emb|CAA49187.1| (X69390) endo-1,
4-beta-glucanase [Clostridium thermocellum]
Length = 566
Score = 167 bits (418), Expect = 3e-40
Identities = 119/394 (30%), Positives = 193/394 (48%), Gaps = 67/394 (17%)
Query: 50 LYLHGVSWFGFELKDHVVYGLDKRNWKD---ILKDVKRLGFNAIRLPFCSESI------R 100
++L G +WFGF ++ +G W D IL + G N +R+P +E + +
Sbjct: 60 VWLTGANWFGFNCSENCFHGA----WYDVKTILTSIADRGINLLRIPISTELLYSWMIGK 115
Query: 101 PDTRPSPERIN----YELNPDLKN------LTSLEIMEKIIEYANSIGLYILLDYHRIGC 150
P+ S N + +NPD + S+EI + I+ Y +G+ +++D H
Sbjct: 116 PNPVSSVTASNNPPYHVVNPDFYDPETDDVKNSMEIFDIIMGYCKELGIKVMIDIHSPDA 175
Query: 151 EEIE---PLWYTENYSE------EQYIKDWIFLAKRFGKYPNVIGADIKNEPHGEAGW-- 199
LWY + S + +I ++LA ++ +I D+KNEPHG+ G+
Sbjct: 176 NNSGHNYELWYGKETSTCGVVTTKMWIDTLVWLADKYKNDDTIIAFDLKNEPHGKRGYTA 235
Query: 200 ----------GTGDERDFRLFAEKVGREILKVAPHWLIFVEGTQ--------YTH-VPNI 240
+ DE +++ AE + IL+V P LI +EG + YT+ P+I
Sbjct: 236 EVPKLLAKWDNSTDENNWKYAAETCAKAILEVNPKVLIVIEGVEQYPKTEKGYTYDTPDI 295
Query: 241 DEIIEKKGWW--TFWGENLMGVKDYPVRLP--RGKVVYSPHVYGPSVYMMDYFKSPDFPN 296
W +WG NL GVKDYP+ L ++VYSPH YGPSVY +F+ DF
Sbjct: 296 WGATGDASPWYSAWWGGNLRGVKDYPIDLGPLNSQIVYSPHDYGPSVYAQPWFEK-DFTM 354
Query: 297 NMPI--IWETHFGYLTDLNYT-LVIGEWGGNYEG-LDKVWQDAFVKWLIKKKIYNFFYWC 352
+ W + Y+ D ++IGEWGG+ +G ++ W ++++ +I++ F WC
Sbjct: 355 QTLLDDYWYDTWAYIHDQGIAPILIGEWGGHMDGGKNQKWMTLLRDYIVQNRIHHTF-WC 413
Query: 353 LNPESGDTGGIFLDDWKTVNWEKMRVIYRLIKAA 386
+NP SGDTGG+ +DW T W++ + Y L+K A
Sbjct: 414 INPNSGDTGGLLGNDWST--WDEAK--YALLKPA 443
>emb|CAB06784.1| (Z86104) endo-1,4-beta-glucanase [Anaerocellum thermophilum]
Length = 341
Score = 166 bits (416), Expect = 6e-40
Identities = 112/338 (33%), Positives = 171/338 (50%), Gaps = 31/338 (9%)
Query: 105 PSPERINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDYHRIGCE---EIEPLWY-TE 160
P P INY +NP+L+ SLE+ + +++ +GL I+LD H I + I P+WY +
Sbjct: 4 PKPN-INYYVNPELEGKNSLEVFDIVVQICKEVGLKIMLDIHSIKTDAMGHIYPVWYGLD 62
Query: 161 NYSEEQYIKDWIFLAKRFGKYPNVIGADIKNEPHGE-------AGWGTG-DERDFRLFAE 212
++ E + K ++ R+ +I D+KNEPHG+ A W D +++ AE
Sbjct: 63 KFTPEDFYKACEWITNRYKNDDTIIAFDLKNEPHGKPWQDTTFAKWDNSTDINNWKYAAE 122
Query: 213 KVGREILKVAPHWLIFVEGTQYTHVPNIDEIIEKKGWW----TFWGENLMGVKDYPVRLP 268
+ IL + P+ LI +EG + P D K + T+WG NL GVK YP+ L
Sbjct: 123 TCAKRILNINPNLLIVIEGIEA--YPKDDVTWTSKSYSDYYSTWWGGNLRGVKKYPINLG 180
Query: 269 R--GKVVYSPHVYGPSVYMMDYFKSPDFPNNMPI--IWETHFGYLTDLNYT-LVIGEWGG 323
+ KVVYSPH YGPSVY +F P F + W ++ Y+ + N L+IGEWGG
Sbjct: 181 KYQNKVVYSPHDYGPSVYQQPWFY-PGFTKESLLQDCWRPNWAYIMEENIAPLLIGEWGG 239
Query: 324 NYEGLD-KVWQDAFVKWLIKKKIYNFFYWCLNPESGDTGGIFLDDWKTVNWEKMRVIYRL 382
+G D + W ++I+ I++ F WC N SGDTGG+ D+ T W++ + Y
Sbjct: 240 YLDGADNEKWMRYLRDYIIENHIHHTF-WCFNANSGDTGGMVGYDFTT--WDEKK--YSF 294
Query: 383 IKAANPEFEEPLYIILKTNATTSILGVGERIRIYWYTN 420
+K A + + ++ L G I IY+ N
Sbjct: 295 LKPALWQDSQGRFVGLDHKRPLGTNGKNINITIYYNNN 332
>sp|P04956|GUNB_CLOTM ENDOGLUCANASE B PRECURSOR (EGB) (ENDO-1,4-BETA-GLUCANASE)
(CELLULASE B) >gi|67395|pir||CZCLBM cellulase (EC
3.2.1.4) B precursor - Clostridium thermocellum
>gi|40669|emb|CAA27266.1| (X03592) endoglucanase B
(celB) (aa 1-563) [Clostridium thermocellum]
Length = 563
Score = 166 bits (415), Expect = 8e-40
Identities = 124/426 (29%), Positives = 205/426 (48%), Gaps = 70/426 (16%)
Query: 3 IKLFCVFIVFIILFSPFVIALSYPDVNYTAENGI----------IFVQNVTTGEK--KPL 50
+K F V ++ +I+ + ++ P V +AE + V+ +K +
Sbjct: 1 MKKFLVLLIALIMIATLLVV---PGVQTSAEGSYADLAEPDDDWLHVEGTNIVDKYGNKV 57
Query: 51 YLHGVSWFGFELKDHVVYGLDKRNWKDILKDVKRL---GFNAIRLPFCSESIRPDTR--- 104
++ G +WFGF ++ ++ LD + DI+ D++ + G N +R+P ++ + ++
Sbjct: 58 WITGANWFGFNCRERML--LDSYH-SDIIADIELVADKGINVVRMPIATDLLYAWSQGIY 114
Query: 105 PSPERINYELNPDLKNLTSLEIMEKIIEYANSIGLYILLDYHRIGCEEIE---PLWYTEN 161
P +Y NP L L S E+ ++E +G+ ++LD H + PLWY
Sbjct: 115 PPSTDTSYN-NPALAGLNSYELFNFMLENFKRVGIKVILDVHSPETDNQGHNYPLWYNTT 173
Query: 162 YSEEQYIKDWIFLAKRFGKYPNVIGADIKNEPHGEAG----------WGTGDE-RDFRLF 210
+EE + K W+++A+R+ +IG D+KNEPH G W + +++
Sbjct: 174 ITEEIFKKAWVWVAERYKNDDTIIGFDLKNEPHTNTGTMKIKAQSAIWDDSNHPNNWKRV 233
Query: 211 AEKVGREILKVAPHWLIFVEGTQYTHVPNI--DEIIEKKGW-------WTFWGENLMGVK 261
AE+ IL+V P+ LIFVEG + I DE + W +WG NL GVK
Sbjct: 234 AEETALAILEVHPNVLIFVEGVEMYPKDGIWDDETFDTSPWTGNNDYYGNWWGGNLRGVK 293
Query: 262 DYPVRLPR--GKVVYSPHVYGPSVYMMDYFKSPDFPNN--------MPIIWETHFGYLTD 311
DYP+ L + ++VYSPH YGP VY D+FK N W ++ Y+ +
Sbjct: 294 DYPINLGKYQSQLVYSPHDYGPIVYEQDWFKGDFITANDEQAKRILYEQCWRDNWAYIME 353
Query: 312 LNYT-LVIGEWGGNYEGLDKVWQDAFVKWL---------IKKKIYNFFYWCLNPESGDTG 361
+ L++GEWGG EG + D +K+L K K+++ F WC+N +S DTG
Sbjct: 354 EGISPLLLGEWGGMTEGGHPL-LDLNLKYLRCMRDFILENKYKLHHTF-WCINIDSADTG 411
Query: 362 GIFLDD 367
G+F D
Sbjct: 412 GLFTRD 417
>gb|AAD48494.1|AF172345_1 (AF172345) endo-1,4-beta-glucanase [Cellulomonas flavigena]
Length = 535
Score = 155 bits (389), Expect = 8e-37
Identities = 101/339 (29%), Positives = 172/339 (49%), Gaps = 28/339 (8%)
Query: 68 YGLDKRNWKDILKDVKRLGFNAIRLPFCSESI---RPDTRPSPERINYELNPDLKNLTSL 124
+GL N++D+ + + G N +R+P ++ + + +N +NP+L TSL
Sbjct: 93 HGLWAVNFEDVTRSMPEHGINIVRVPISTQLLLEWKAGKAAVSSGVNTYVNPELVGKTSL 152
Query: 125 EIMEKIIEYANSIGLYILLDYHRIGCEE---IEPLWYTENYSEEQYIKDWIFLAKRFGKY 181
E+ + + + GL ++LD H + P+W+ + + + W ++ R+
Sbjct: 153 EVFGRFLALSEKYGLKVMLDVHSAEADNAGHFHPVWWKGTVTVDDFYSAWEWVTARYKNN 212
Query: 182 PNVIGADIKNEPHGEAG------WGTGDERD-FRLFAEKVGREILKVAPHWLIFVEGTQY 234
++ DIKNEPHG A W + ++D F+ + GR+IL + P+ LI EG +
Sbjct: 213 DTLVAMDIKNEPHGTANSSPRAKWDSSTDQDNFKNLCQVTGRKILAINPNVLILCEGIEV 272
Query: 235 THVPNIDEIIEKKGWW-TFWGENLMGVKDYPVRL--PRGKVVYSPHVYGPSVYMMDYFKS 291
P + K ++ T+WG NL GV D+PV L + ++ SPH YGP ++ +
Sbjct: 273 YPKPGVSWTSTNKEFYGTWWGCNLRGVTDHPVDLCANQDQLGDSPHDYGPMMFGSRTGST 332
Query: 292 PDFPNNM--PIIWETHFGYLTDLNYT-LVIGEWGGNY---EGLDKVWQDAFVKWLIKKKI 345
P +W+ ++ Y+ N + L+I EWGG E D+ W A +I++++
Sbjct: 333 SRSPRLTLEADVWDPNWLYIHKKNISQLLIREWGGQVAQDERQDR-WMTALRDLMIERRM 391
Query: 346 YNFFYWCLNPESGDTGGIFLDDWKTVNWEKMRVIYRLIK 384
+ F W LNP SGDTGG+ LDDWKT W+ + Y+L+K
Sbjct: 392 LHTF-WSLNPNSGDTGGLLLDDWKT--WDSAK--YQLLK 425
>pir||B82761 extracellular endoglucanase precursor XF0810 [imported] - Xylella
fastidiosa (strain 9a5c)
>gi|9105708|gb|AAF83620.1|AE003920_11 (AE003920)
extracellular endoglucanase precursor [Xylella
fastidiosa]
Length = 565
Score = 118 bits (292), Expect = 2e-25
Identities = 73/235 (31%), Positives = 117/235 (49%), Gaps = 13/235 (5%)
Query: 52 LHGVSWFGFELKDHVVYGLDKRNWKDILKDVKRLGFNAIRLPFCSESIRPDTRPSPERIN 111
LHGV+W G E H++ GLD+R W+++L ++ LG NAIRLP CS+++ P ++
Sbjct: 21 LHGVNWRGLETAQHLLQGLDQRPWREVLDQMQSLGINAIRLPLCSDTLH---GTMPTNLD 77
Query: 112 YELNPDLKNLTSLEIMEKIIEYANSIGLYILLDYHRIGC-EEIEPLWYTENYSEEQYIKD 170
NPDLK T+L+I + II+ A G+ +LL YH + C + PL + + SE Q+I D
Sbjct: 78 LVRNPDLKGRTALQIADAIIDEAGKRGMRVLLAYHGVECPTDGNPLLRSVDESEHQWISD 137
Query: 171 WIFLAKRFGKYPNVI-GADIKNEPHGEAGWGTGDER-DFRLFAEKVGREILKVAPHWLIF 228
F+ + V+ G D+ + + GD D+ E+ IL + P WLI
Sbjct: 138 VQFITSHYRAQQKVVMGVDLADMANHRPFQSGGDSTPDWNRVVERAAAAILAMTPEWLIG 197
Query: 229 VEGTQYTHVPNIDEIIEKKGWWTFWGENLMGVKDYPVRLPRGKVVYSPHVYGPSV 283
V+ + P +D +N+ P+R+P ++ PH G +
Sbjct: 198 VQPVG-LNPPCLDASA------PISDDNIQSQHCVPLRIPARNLLLMPHFAGTDI 245
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.322 0.143 0.454
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 218106393
Number of Sequences: 2977
Number of extensions: 10403372
Number of successful extensions: 22044
Number of sequences better than 1.0e-10: 23
Number of HSP's better than 0.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 21915
Number of HSP's gapped (non-prelim): 26
length of query: 514
length of database: 189,106,746
effective HSP length: 50
effective length of query: 464
effective length of database: 159,182,396
effective search space: 73860631744
effective search space used: 73860631744
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 167 (69.5 bits)