BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0640 (hydD-2) DE:HYDROGENASE (EC 1.12.2.1) (CYTOCHROME-C3
HYDROGENASE DELTA CHAIN)
(241 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||A75070 cytochrome-c3 hydrogenase (EC 1.12.2.1) delta chain ... 501 e-141
gb|AAF61853.1|AF176650_3 (AF176650) sulfhydrogenase II subunit d... 392 e-108
pir||S48835 cytochrome-c3 hydrogenase (EC 1.12.2.1) delta chain ... 183 2e-45
pir||A71075 probable cytochrome-c3 hydrogenase delta chain - Pyr... 182 3e-45
pir||C75133 cytochrome-c3 hydrogenase delta chain PAB1786 - Pyro... 182 5e-45
gb|AAB94935.1| (AF039208) NiFe hydrogenase delta subunit [Thermo... 164 7e-40
pir||F64448 methylviologen-reducing hydrogenase gamma chain homo... 111 1e-23
pir||S24800 methylviologen-reducing hydrogenase chain vhuG - Met... 106 3e-22
pir||C59304 methylviologen-reducing hydrogenase gamma chain homo... 106 4e-22
gi|11498969 methylviologen-reducing hydrogenase, subunit gamma (... 100 3e-20
sp|P22319|HOXY_ALCEU NAD-REDUCING HYDROGENASE HOXS DELTA SUBUNIT... 78 1e-13
sp|Q60340|FRHG_METJA COENZYME F420 HYDROGENASE GAMMA SUBUNIT (8-... 78 1e-13
emb|CAA56463.1| (X80180) hydrogenase small beta subunit [Acetomi... 77 2e-13
gb|AAB57891.1| (U70364) NAD-reducing hydrogenase delta subunit [... 77 2e-13
pir||S16726 methylviologen-reducing hydrogenase chain vhcG - Met... 75 1e-12
pir||S32834 methylviologen-reducing hydrogenase chain vhcG [simi... 72 7e-12
pir||S24797 coenzyme F420-reducing hydrogenase chain furG [simil... 70 3e-11
pir||I39733 hydrogenase small chain - Anabaena variabilis >gi|10... 69 5e-11
>pir||A75070 cytochrome-c3 hydrogenase (EC 1.12.2.1) delta chain PAB0640 -
Pyrococcus abyssi (strain Orsay)
>gi|5458374|emb|CAB49862.1| (AJ248286) HYDROGENASE (EC
1.12.2.1) (CYTOCHROME-C3 HYDROGENASE DELTA CHAIN)
[Pyrococcus abyssi]
Length = 241
Score = 501 bits (1275), Expect = e-141
Identities = 241/241 (100%), Positives = 241/241 (100%)
Query: 1 MDKLKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFHMATSQRGREKLDVALVTGS 60
MDKLKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFHMATSQRGREKLDVALVTGS
Sbjct: 1 MDKLKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFHMATSQRGREKLDVALVTGS 60
Query: 61 VSTQRDLEVVKDARNRAEYLIALGTCATHGGVQGSIEGSVKEGLRKIYGDMKGPSKVLEP 120
VSTQRDLEVVKDARNRAEYLIALGTCATHGGVQGSIEGSVKEGLRKIYGDMKGPSKVLEP
Sbjct: 61 VSTQRDLEVVKDARNRAEYLIALGTCATHGGVQGSIEGSVKEGLRKIYGDMKGPSKVLEP 120
Query: 121 RAVVEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLECKLNEYECVLLKRG 180
RAVVEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLECKLNEYECVLLKRG
Sbjct: 121 RAVVEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLECKLNEYECVLLKRG 180
Query: 181 VPCLGPVTLGGCNAKCPSIGLGCIGCRGLVPDPNIPGLVEVLKNIIPEEDIVRKLKTFVR 240
VPCLGPVTLGGCNAKCPSIGLGCIGCRGLVPDPNIPGLVEVLKNIIPEEDIVRKLKTFVR
Sbjct: 181 VPCLGPVTLGGCNAKCPSIGLGCIGCRGLVPDPNIPGLVEVLKNIIPEEDIVRKLKTFVR 240
Query: 241 W 241
W
Sbjct: 241 W 241
>gb|AAF61853.1|AF176650_3 (AF176650) sulfhydrogenase II subunit d [Pyrococcus furiosus]
Length = 237
Score = 392 bits (996), Expect = e-108
Identities = 179/238 (75%), Positives = 211/238 (88%), Gaps = 1/238 (0%)
Query: 4 LKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFHMATSQRGREKLDVALVTGSVST 63
+KLGVFELT CGGCALN+LFLY++L D+LEFY+IAEFHMATS++ REK+DVALVTG+VST
Sbjct: 1 MKLGVFELTDCGGCALNLLFLYDKLLDLLEFYEIAEFHMATSKKSREKIDVALVTGTVST 60
Query: 64 QRDLEVVKDARNRAEYLIALGTCATHGGVQGSIEGSVKEGLRKIYGDMKGPSKVLEPRAV 123
QRDLEV++DARNR+EYLIALGTCATHG VQG IE S KE R++YG+ K P K+L P+ V
Sbjct: 61 QRDLEVLRDARNRSEYLIALGTCATHGSVQGVIENS-KEAYRRVYGNGKPPVKLLNPKPV 119
Query: 124 VEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLECKLNEYECVLLKRGVPC 183
++VPVDFAIPGCPYDK+EVFQVL+DIA+G+EPV KDYPVCLECKLNEYECVLLK+ +PC
Sbjct: 120 TDYVPVDFAIPGCPYDKEEVFQVLIDIAKGIEPVAKDYPVCLECKLNEYECVLLKKRIPC 179
Query: 184 LGPVTLGGCNAKCPSIGLGCIGCRGLVPDPNIPGLVEVLKNIIPEEDIVRKLKTFVRW 241
LGPVT GGCNAKCPS GLGCIGCRG D N+PG+ EVLK I+P+E+I RKL+TF RW
Sbjct: 180 LGPVTAGGCNAKCPSYGLGCIGCRGPSLDNNVPGMFEVLKEILPDEEIARKLRTFARW 237
>pir||S48835 cytochrome-c3 hydrogenase (EC 1.12.2.1) delta chain - Pyrococcus
furiosus >gi|562776|emb|CAA53036.1| (X75255) hydrogenase
(delta subunit) [Pyrococcus furiosus]
Length = 261
Score = 183 bits (460), Expect = 2e-45
Identities = 95/241 (39%), Positives = 141/241 (58%), Gaps = 3/241 (1%)
Query: 1 MDKLKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFHMATSQRGREKLDVALVTGS 60
M K+++G + LTSC GC L + + E L I + F + EK+D+A + GS
Sbjct: 1 MGKVRIGFYALTSCYGCQLQLAMMDELLQLIPNAEIVCWFMIDRDSIEDEKVDIAFIEGS 60
Query: 61 VSTQRDLEVVKDARNRAEYLIALGTCATHGGVQGSIEGSVKEGLRKIYGDMKGPSKVLEP 120
VST+ ++E+VK R A+ ++A+G CA GGVQ E ++E +K+YGD K + +
Sbjct: 61 VSTEEEVELVKKIRENAKIVVAVGACAVQGGVQSWSEKPLEELWKKVYGDAKVKFQPKKA 120
Query: 121 RAVVEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLECKLNEYECVLLKRG 180
V +++ VD+ I GCP +K + L G P DYPVCLEC+LN + C+LL++G
Sbjct: 121 EPVSKYIKVDYNIYGCPPEKKDFLYALGTFLIGSWPEDIDYPVCLECRLNGHPCILLEKG 180
Query: 181 VPCLGPVTLGGCNAKCPSIGLGCIGCRGLV--PDPNIPGLVEVLKNI-IPEEDIVRKLKT 237
PCLGPVT GCNA+CP G+ CIGCRG + L +V K + +E+I+ ++K
Sbjct: 181 EPCLGPVTRAGCNARCPGFGVACIGCRGAIGYDVAWFDSLAKVFKEKGMTKEEIIERMKM 240
Query: 238 F 238
F
Sbjct: 241 F 241
>pir||A71075 probable cytochrome-c3 hydrogenase delta chain - Pyrococcus
horikoshii >gi|3257712|dbj|BAA30395.1| (AP000005) 266aa
long hypothetical cytochrome-c3 hydrogenase delta chain
[Pyrococcus horikoshii]
Length = 266
Score = 182 bits (458), Expect = 3e-45
Identities = 93/239 (38%), Positives = 138/239 (56%), Gaps = 3/239 (1%)
Query: 3 KLKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFHMATSQRGREKLDVALVTGSVS 62
K+++G + LTSC GC L + + E L + + + + E +D+A + GSVS
Sbjct: 8 KIRIGFYALTSCYGCQLQLAMMDELLLLLPHIELVCWYMVDRDSIDDEPVDIAFIEGSVS 67
Query: 63 TQRDLEVVKDARNRAEYLIALGTCATHGGVQGSIEGSVKEGLRKIYGDMKGPSKVLEPRA 122
T+ ++E+VK R ++ ++A+G CA GGVQ + S++E R +YGD K K +
Sbjct: 68 TEEEVELVKKIRENSKIVVAVGACAVQGGVQSWTDKSLEELWRTVYGDAKVKFKPKKAEP 127
Query: 123 VVEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLECKLNEYECVLLKRGVP 182
V +++ VD+ I GCP +K + L G P DYPVCLEC+LN Y CVLL++G P
Sbjct: 128 VSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLECRLNGYPCVLLEKGEP 187
Query: 183 CLGPVTLGGCNAKCPSIGLGCIGCRGLV--PDPNIPGLVEVLKNI-IPEEDIVRKLKTF 238
CLGPVT GCNA+CP G+ CIGCRG + L V K + +E+I+ ++K F
Sbjct: 188 CLGPVTRAGCNARCPGFGIACIGCRGAIGYDVAWFDSLARVFKEKGLTKEEIIERMKIF 246
>pir||C75133 cytochrome-c3 hydrogenase delta chain PAB1786 - Pyrococcus abyssi
(strain Orsay) >gi|5458291|emb|CAB49780.1| (AJ248285)
CYTOCHROME-C3 HYDROGENASE DELTA CHAIN [Pyrococcus
abyssi]
Length = 261
Score = 182 bits (456), Expect = 5e-45
Identities = 95/242 (39%), Positives = 143/242 (58%), Gaps = 5/242 (2%)
Query: 1 MDKLKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFHMATSQRGREK-LDVALVTG 59
M KL++G + LTSC GC L + + E L ++ +I ++M +K +D+A + G
Sbjct: 1 MGKLRIGFYALTSCYGCQLQLAMMDE-LLKLIPNAEIVCWYMLDRDSVEDKPVDIAFIEG 59
Query: 60 SVSTQRDLEVVKDARNRAEYLIALGTCATHGGVQGSIEGSVKEGLRKIYGDMKGPSKVLE 119
SVST+ ++E+VK R A+ ++A+G CA GGVQ + S++E + +YGD K + +
Sbjct: 60 SVSTEEEVELVKKIRENAKIVVAVGACAVQGGVQSWTDKSLEELWKTVYGDAKVKFQPKK 119
Query: 120 PRAVVEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLECKLNEYECVLLKR 179
V +++ VD+ I GCP +K + L G P DYPVCLEC+LN Y CVLL++
Sbjct: 120 AEPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLECRLNGYPCVLLEK 179
Query: 180 GVPCLGPVTLGGCNAKCPSIGLGCIGCRGLV--PDPNIPGLVEVLKNI-IPEEDIVRKLK 236
G PCLGP+T GCNA+CP G+ CIGCRG + L V K + +E+I+ ++K
Sbjct: 180 GEPCLGPITRAGCNARCPGFGIACIGCRGAIGYDVAWFDSLARVFKEKGLTKEEILERMK 239
Query: 237 TF 238
F
Sbjct: 240 IF 241
>gb|AAB94935.1| (AF039208) NiFe hydrogenase delta subunit [Thermococcus litoralis]
Length = 263
Score = 164 bits (412), Expect = 7e-40
Identities = 92/247 (37%), Positives = 141/247 (56%), Gaps = 16/247 (6%)
Query: 2 DKLKLGVFELTSCGGCALNILFLYERLFDILEFYDIAE----FHMATSQRGREKLDVALV 57
+K+++G + LTSC GC L + E IL D AE F + ++D+A +
Sbjct: 4 EKVRIGFYALTSCYGCQLQFAMMDE----ILHLLDKAEIECWFMVERDSDEDREVDIAFI 59
Query: 58 TGSVSTQRDLEVVKDARNRAEYLIALGTCATHGGVQG-SIEGSVKEGLRKIYGD--MKGP 114
GSVSTQ ++E+VK R +A+ ++A+G CAT GGVQ + + E + +YGD +K
Sbjct: 60 EGSVSTQEEVELVKKIREKAKIVVAVGACATQGGVQSWGKDKELSELWKTVYGDAHVKFE 119
Query: 115 SKVLEPRAVVEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLECKLNEYEC 174
K+ EP V +++ VD+ + GCP +K + L G P DYPVC+EC+L C
Sbjct: 120 PKMAEP--VEKYIKVDYKLYGCPPEKKDFLYALGTFLVGSWPEDIDYPVCVECRLRGNPC 177
Query: 175 VLLKRGVPCLGPVTLGGCNAKCPSIGLGCIGCRGLVPDPNI---PGLVEVLKNIIPEEDI 231
+L+++G PCLGPVT+ GC+A+CP + CIGCRG V +E K + +E+I
Sbjct: 178 ILIEKGEPCLGPVTVAGCDARCPGFNVACIGCRGAVGYDVAWFDSLALEFKKKGLTKEEI 237
Query: 232 VRKLKTF 238
+ ++K F
Sbjct: 238 LERMKIF 244
>pir||F64448 methylviologen-reducing hydrogenase gamma chain homolog -
Methanococcus jannaschii >gi|1591819|gb|AAB99193.1|
(U67560) methylviologen-reducing hydrogenase, gamma
chain (vhuG) [Methanococcus jannaschii]
Length = 288
Score = 111 bits (274), Expect = 1e-23
Identities = 73/235 (31%), Positives = 117/235 (49%), Gaps = 30/235 (12%)
Query: 4 LKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFHM-ATSQRGREKLDVALVTGSVS 62
+K+G+ +L C GC +++L L+++L ++L +I + A + E +DV LV G +
Sbjct: 6 VKVGMIQLCGCSGCHISLLDLHDKLLEVLPNLEIVYAPIIADPKEIPEGIDVFLVEGGIR 65
Query: 63 TQRDLEVVKDARNRAEYLIALGTCATHGGVQGSIEGSVKEGLRK-IYG----DMKG--PS 115
+ D ++ + R +++ +IA GTCA +GG+ G KE L +Y + KG PS
Sbjct: 66 NEHDEHLIHEIREKSKIVIAWGTCAAYGGIPGLGNLYKKEELLNYVYSTDSTENKGEIPS 125
Query: 116 KVLEP-----RAVVEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLEC--- 167
+ + P + + + + VD+ IPGCP + ++ + G EP VC EC
Sbjct: 126 EEIPPLEEYVKPIKDFIKVDYTIPGCPPTPKMIADAIIALLNGEEPKLPTKIVCDECPRK 185
Query: 168 KLNEY--------------ECVLLKRGVPCLGPVTLGGCNAKCPSIGLGCIGCRG 208
K N + E L ++G CLG T GC AKCPS G+ C GC G
Sbjct: 186 KENVFPETFKRTHEGRPDPERCLFEQGYTCLGFATRAGCGAKCPSAGVPCRGCFG 240
>pir||S24800 methylviologen-reducing hydrogenase chain vhuG - Methanococcus
voltae
Length = 287
Score = 106 bits (262), Expect = 3e-22
Identities = 71/237 (29%), Positives = 111/237 (45%), Gaps = 30/237 (12%)
Query: 2 DKLKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFHMATSQRGREKLDVALVTGSV 61
DK++LG+ +L C GC +++L L+E+L D+L +I + + + DV L+ G
Sbjct: 3 DKVRLGLIQLCGCSGCHISLLDLHEQLLDVLPNLEIVYAPIIADVKEIPECDVFLIEGGA 62
Query: 62 STQRDLEVVKDARNRAEYLIALGTCATHGGVQGSIEGSVKEGLRK-IYG----DMKG--P 114
+ D ++ + R +++ +IA GTCA +GG+ G E L+K +YG D G P
Sbjct: 63 RNEHDEHLIHEIREKSKVVIAWGTCAVYGGIPGLGNLYSAEQLKKTVYGTETTDNVGELP 122
Query: 115 SKVLEP------RAVVEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLECK 168
S + P V V V++ IPGCP + ++ + G +P VC EC
Sbjct: 123 SDEMVPPLTNSVMPVPSIVDVEYVIPGCPPRPEINAGAIVALLEGRDPELPQKIVCDECP 182
Query: 169 LN-----------------EYECVLLKRGVPCLGPVTLGGCNAKCPSIGLGCIGCRG 208
+ E L ++G C+G T GC A CPS G+ C GC G
Sbjct: 183 RTKENVIPETFKRTFEGTPDNEKCLFEQGYTCVGMGTRAGCGALCPSAGVPCRGCYG 239
>pir||C59304 methylviologen-reducing hydrogenase gamma chain homolog vhuG
[similarity] - Methanococcus voltae
>gi|1747407|emb|CAA43509.1| (X61204)
methylviologen-reducing hydrogenase subunit
[Methanococcus voltae]
Length = 287
Score = 106 bits (261), Expect = 4e-22
Identities = 70/237 (29%), Positives = 111/237 (46%), Gaps = 30/237 (12%)
Query: 2 DKLKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFHMATSQRGREKLDVALVTGSV 61
DK++LG+ +L C GC +++L L+E+L D+L +I + + + DV L+ G
Sbjct: 3 DKVRLGLIQLCGCSGCHISLLDLHEQLLDVLPNLEIVYAPIIADVKEIPECDVFLIEGGA 62
Query: 62 STQRDLEVVKDARNRAEYLIALGTCATHGGVQGSIEGSVKEGLRK-IYG----DMKG--P 114
+ D ++ + R +++ +IA GTCA +GG+ G E L+K +YG D G P
Sbjct: 63 RNEHDEHLIHEIREKSKVVIAWGTCAVYGGIPGLGNLYSAEQLKKTVYGTETTDNVGELP 122
Query: 115 SKVLEP------RAVVEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLECK 168
S + P V V V++ IPGCP + ++ + G +P VC EC
Sbjct: 123 SDEMVPPLTNSVMPVPSIVDVEYVIPGCPPRPEINAGAIVALLEGRDPELPKKIVCDECP 182
Query: 169 LNEYECV-----------------LLKRGVPCLGPVTLGGCNAKCPSIGLGCIGCRG 208
+ + L ++G C+G T GC A CPS G+ C GC G
Sbjct: 183 RTKENVIPETFKRTFEGTPDPEKCLFEQGYTCVGMGTRAGCGALCPSAGVPCRGCYG 239
>gi|11498969 methylviologen-reducing hydrogenase, subunit gamma (vhuG)
[Archaeoglobus fulgidus] >gi|7483871|pir||D69421
methylviologen-reducing hydrogenase, subunit gamma
(vhuG) homolog - Archaeoglobus fulgidus
>gi|2649199|gb|AAB89871.1| (AE001009)
methylviologen-reducing hydrogenase, subunit gamma
(vhuG) [Archaeoglobus fulgidus]
Length = 293
Score = 99.8 bits (245), Expect = 3e-20
Identities = 69/245 (28%), Positives = 112/245 (45%), Gaps = 28/245 (11%)
Query: 14 CGGCALNILFLYERLFDILEFYDIAEFHMATSQRGR-EKLDVALVTGSVSTQRDLEVVKD 72
C GC +++L L ER+ D A + + R ++D A + G + +E+V++
Sbjct: 12 CSGCTMSMLDLGERMNDFRMRVAWAAPTLGDGKYDRIPEVDYAFIEGGIGLSEHVEIVRE 71
Query: 73 ARNRAEYLIALGTCATHGGVQGSIEGSVKEGL--------RKIYGDMKGPSKVLEPRAVV 124
R ++E L+A G CA+ GG+ G KE + + GD++ P + + +
Sbjct: 72 LREKSETLVAFGICASCGGIMGLANLHSKEEILEDVFGSAKSSVGDLRIPEMLEFVKPLD 131
Query: 125 EHVPVDFAIPGCPYDKDEVFQVLMDIARGVEP-VTKDYPVCLECKLN------------- 170
V VD I GCP ++++ I G +T VC C N
Sbjct: 132 SVVEVDCYIGGCPPTPEQIYNAFKAIFEGERGWITSGKSVCEFCSRNLSEKFGEVSRFLR 191
Query: 171 --EYECVLLKRGVPCLGPVTLGGCNAKCPSIGLGCIGCRGLVP--DPNIPGLVEVLKNII 226
+ EC LK+G+ C GP T G C A CP+ C GC G++P D + LV++L ++
Sbjct: 192 VPDKEC-FLKQGILCFGPATQGDCMASCPNSNSPCRGCGGMLPSVDDHGSALVDMLSSLT 250
Query: 227 PEEDI 231
E+ I
Sbjct: 251 DEKGI 255
>sp|P22319|HOXY_ALCEU NAD-REDUCING HYDROGENASE HOXS DELTA SUBUNIT >gi|95478|pir||C35385
hydrogen dehydrogenase (EC 1.12.1.2) delta chain -
Alcaligenes eutrophus >gi|141949|gb|AAC06142.1| (M55230)
NAD-reducing hydrogenase [Ralstonia eutropha]
Length = 209
Score = 78.0 bits (189), Expect = 1e-13
Identities = 55/189 (29%), Positives = 87/189 (45%), Gaps = 27/189 (14%)
Query: 3 KLKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFHMATSQRGREKLDVALVTGSVS 62
K+K+ L C GC L+ L + ERL +LE + + +R E+ + V G VS
Sbjct: 30 KIKVATIGLCGCWGCTLSFLDMDERLLPLLEKVTLLRSSLTDIKRIPERCAIGFVEGGVS 89
Query: 63 TQRDLEVVKDARNRAEYLIALGTCATHGGVQGSIE-GSVKEGLRKIYGDMKGPSKVLEPR 121
++ ++E ++ R + LI++G CA GGV +K+ L + Y + + V +
Sbjct: 90 SEENIETLEHFRENCDILISVGACAVWGGVPAMRNVFELKDCLAEAY--VNSATAVPGAK 147
Query: 122 AVV-----------------EHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVC 164
AVV E V +D+ IPGCP D D +F+VL D+ G P
Sbjct: 148 AVVPFHPDIPRITTKVYPCHEVVKMDYFIPGCPPDGDAIFKVLDDLVNG-------RPFD 200
Query: 165 LECKLNEYE 173
L +N Y+
Sbjct: 201 LPSSINRYD 209
>sp|Q60340|FRHG_METJA COENZYME F420 HYDROGENASE GAMMA SUBUNIT
(8-HYDROXY-5-DEAZAFLAVIN-REDUCING HYDROGENASE GAMMA
SUBUNIT) (FRH) >gi|2127831|pir||G64303 coenzyme F420
hydrogenase gamma chain homolog - Methanococcus
jannaschii >gi|1590833|gb|AAB98010.1| (U67461) coenzyme
F420-reducing hydrogenase, gamma subunit [Methanococcus
jannaschii]
Length = 230
Score = 78.0 bits (189), Expect = 1e-13
Identities = 58/200 (29%), Positives = 89/200 (44%), Gaps = 19/200 (9%)
Query: 4 LKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFHMATSQRGREKLDVALVTGSV-- 61
+K+ +L SC GC +++ YE+L D+L ++ R + D+ALV GSV
Sbjct: 5 VKVAHVQLCSCCGCLVSLADTYEKLLDVLNSIELVYCQTLADAREIPECDIALVEGSVCL 64
Query: 62 STQRDLEVVKDARNRAEYLIALGTCATHGGVQGSIEGSVKEGLRKIYGDMKGPSKVLEPR 121
LEV ++ R +A+ ++ALG CA GGV +G+ L K P
Sbjct: 65 DDHHSLEVAQEVRKKAKIVVALGACAATGGVTRYCKGN---QLSKPVHSSFSP------- 114
Query: 122 AVVEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLECKLNEYECVLLKRGV 181
+ E + VD AIPGCP + + V+ G + Y E C ++ + V
Sbjct: 115 -LTEVIKVDLAIPGCPPSPEAIVGVITAALNGDMEYLQPYAELAEKGSEACGCDVIYKVV 173
Query: 182 P---CLGPVTLGGCNAKCPS 198
C+G G C A CP+
Sbjct: 174 NKSLCMG---CGTCAAACPT 190
>emb|CAA56463.1| (X80180) hydrogenase small beta subunit [Acetomicrobium flavidum]
Length = 179
Score = 77.3 bits (187), Expect = 2e-13
Identities = 48/170 (28%), Positives = 77/170 (45%), Gaps = 15/170 (8%)
Query: 1 MDKLKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFHMATSQRGREKLDVALVTGS 60
M K K+ F L +C GC ++ L L ERL D+ + +I F + +DV +++G
Sbjct: 1 MAKAKVATFWLEACAGCHMSFLDLDERLIDLFQNVEIL-FSPIVDAKDIPNIDVGVLSGG 59
Query: 61 VSTQRDLEVVKDARNRAEYLIALGTCATHGGV--------------QGSIEGSVKEGLRK 106
+ ++E+ K R R +YL+A G CA GG+ +G IE + +
Sbjct: 60 LGNVEEVELAKKMRERCKYLVAWGDCAVFGGINCMRNFIPKDVVLREGYIETASTVNPQG 119
Query: 107 IYGDMKGPSKVLEPRAVVEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEP 156
I P + + V VD +PGCP D D ++ V ++ G P
Sbjct: 120 IVPSEDIPELLPRALPIDYEVKVDVYVPGCPPDADTIYYVFKELLAGRVP 169
>gb|AAB57891.1| (U70364) NAD-reducing hydrogenase delta subunit [Rhodococcus
opacus]
Length = 209
Score = 77.3 bits (187), Expect = 2e-13
Identities = 53/187 (28%), Positives = 90/187 (47%), Gaps = 23/187 (12%)
Query: 3 KLKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFHMATSQRGREKLDVALVTGSVS 62
K+K+ + L C GC L+ L + ERL +L+ + ++ +R E+ + + G V+
Sbjct: 30 KIKVAMIGLCGCWGCTLSFLDMDERLLVLLDKVTLHRSSLSDIKRITERCAIGFIEGGVA 89
Query: 63 TQRDLEVVKDARNRAEYLIALGTCATHGGVQGSIE-GSVKEGLRKIYGD----MKGPSKV 117
+ ++E ++ R + LI++G CA GGV +K+ L ++Y D + G V
Sbjct: 90 NEENIETLEHYRENCDVLISVGACAVWGGVPAMRNVFELKDCLSEVYIDSATSVPGAKPV 149
Query: 118 LE-----PRAV------VEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLE 166
+ PR E V +D+ IPGCP D D +F+VL D+ G P L
Sbjct: 150 VPFHPDIPRITDKVYPCHEVVKMDYFIPGCPPDADAIFKVLDDLVNG-------RPFDLP 202
Query: 167 CKLNEYE 173
+N+Y+
Sbjct: 203 SSINQYD 209
>pir||S16726 methylviologen-reducing hydrogenase chain vhcG - Methanococcus
voltae
Length = 289
Score = 74.5 bits (180), Expect = 1e-12
Identities = 55/227 (24%), Positives = 98/227 (42%), Gaps = 46/227 (20%)
Query: 14 CGGCALNILFLYERLFDILEFYDIAEFHMATSQRG-REKLDVALVTGSVSTQRDLEVVKD 72
C GC ++++ L+E L DILE ++ + R + +DVAL+ G V + +L + ++
Sbjct: 12 CSGCHISLIDLHEDLLDILEQVELIHSPVLMDVREIPDDIDVALIEGGVRSGENLHIARE 71
Query: 73 ARNRAEYLIALGTCATHGGVQGSIEGSVKEGLRKIYGDMKGPSKVLEPRAVV-------- 124
R A+ +IA GTCA +GG+ G G++ +++LE V+
Sbjct: 72 MREEAKVVIAFGTCAVYGGIPG-------------MGNLYSTAELLEKAPVLHLTSRVTP 118
Query: 125 --EHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLEC--------------- 167
+ + VD+ P + + VL + EP + +C C
Sbjct: 119 LSQVIDVDYIFQDAPPKPELIASVLTALLEEKEPELSNKNMCEVCPREKSSEGVVVGELK 178
Query: 168 -----KLNEYECVLLKRGVPCLGPVTLGGCNAKCPSI-GLGCIGCRG 208
+++ +C LL++G C+G T C C + + C GC G
Sbjct: 179 RNYEGEIDPKKC-LLEQGYVCMGVATRASCRCFCALVRHVPCTGCYG 224
>pir||S32834 methylviologen-reducing hydrogenase chain vhcG [similarity] -
Methanococcus voltae >gi|296615|emb|CAA43505.1| (X61203)
methylviologen reducing hydrogenase subunit
[Methanococcus voltae]
Length = 306
Score = 71.8 bits (173), Expect = 7e-12
Identities = 57/231 (24%), Positives = 98/231 (41%), Gaps = 37/231 (16%)
Query: 14 CGGCALNILFLYERLFDILEFYDIAEFHMATSQRG-REKLDVALVTGSVSTQRDLEVVKD 72
C GC ++++ L+E L DILE ++ + R + +DVAL+ G V + +L + ++
Sbjct: 12 CSGCHISLIDLHEDLLDILEQVELIHSPVLMDVREIPDDIDVALIEGGVRSGENLHIARE 71
Query: 73 ARNRAEYLIALGTCATHGGVQGSIE-GSVKEGLRKIY-------------GDMKGPSKVL 118
R A+ +IA GTCA +GG+ G S +E L K Y + P+
Sbjct: 72 MREEAKVVIAFGTCAVYGGIPGMGNLYSNEELLEKAYKTTITTKNDEGIIPSEEVPALTS 131
Query: 119 EPRAVVEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLEC----------- 167
+ + + VD+ P + + VL + EP + +C C
Sbjct: 132 RVTPLSQVIDVDYIFQDAPPKPELIASVLTALLEEKEPELSNKNMCEVCPREKSSEGVVV 191
Query: 168 ---------KLNEYECVLLKRGVPCLGPVTLGGCNAKCPSI-GLGCIGCRG 208
+++ +C LL++G C+G T C C + + C GC G
Sbjct: 192 GELKRNYEGEIDPKKC-LLEQGYVCMGVATRASCRCFCALVRHVPCTGCYG 241
>pir||S24797 coenzyme F420-reducing hydrogenase chain furG [similarity] -
Methanococcus voltae >gi|44707|emb|CAA43502.1| (X61202)
F420-reducing hydrogenase subunit [Methanococcus voltae]
Length = 228
Score = 69.9 bits (168), Expect = 3e-11
Identities = 62/241 (25%), Positives = 98/241 (39%), Gaps = 35/241 (14%)
Query: 4 LKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFH-MATSQRGREKLDVALVTGSV- 61
+K+ +L+SC GC +++ YE+L D+L D+ +A + + +D+ L+ GSV
Sbjct: 2 VKVAHVQLSSCCGCLVSLTDTYEKLLDVLGAIDLVYSQTLADVREVPDDVDIILLEGSVC 61
Query: 62 -STQRDLEVVKDARNRAEYLIALGTCATHGGVQGSIEGSVKEGLRKIYGDMKGPSKVLEP 120
S LE R +A+ L+ALG CA G + G + K D P
Sbjct: 62 LSDHHALETALACREKAKILVALGACAASGNITRFSRGG---QMSKPVHDAFAP------ 112
Query: 121 RAVVEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLECKLNEYECVLLKRG 180
+ E V D AIPGCP + + V+ G + Y + C L+ +
Sbjct: 113 --LTEVVKCDLAIPGCPPSPESIVAVITAALEGDMDYLEPYAELAKYGSEACGCDLIVKV 170
Query: 181 VP---CLGPVTLGGCNAKCPS---------------IGLGCIGCRGLVPDPNIPGLVEVL 222
+ C+G G C A CP+ I + C C P P L+E +
Sbjct: 171 INKSLCMG---CGACAAACPTRAVTMECGRPAIDKEICIKCGACSVQCPRIRFPELIEKI 227
Query: 223 K 223
+
Sbjct: 228 E 228
>pir||I39733 hydrogenase small chain - Anabaena variabilis
>gi|1032479|emb|CAA55876.1| (X79285) hydrogenase small
subunit [Anabaena variabilis]
Length = 205
Score = 69.1 bits (166), Expect = 5e-11
Identities = 52/170 (30%), Positives = 75/170 (43%), Gaps = 14/170 (8%)
Query: 1 MDKLKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFHMATSQRGREKLDVALVTGS 60
M +LKL L C GC ++ L L E L D+ D+ A + E +DV LV G+
Sbjct: 25 MSRLKLATVWLGGCSGCHMSFLDLDEWLIDLAAQADVVFSPFADIKEYPEGVDVVLVEGA 84
Query: 61 VSTQRDLEVVKDARNRAEYLIALGTCATHGGVQG--SIEGSVKEGLRKIYGDMK--GPSK 116
++ + L +K R R++ LI+ G CA G V + GS + L++ Y P
Sbjct: 85 IANEEHLTTIKIVRERSQILISFGDCAVTGNVTALRNPLGSAEPVLQRCYIQAVDINPQI 144
Query: 117 VLEP--------RAVVEH--VPVDFAIPGCPYDKDEVFQVLMDIARGVEP 156
EP R H VPVD +PGCP + L + +G P
Sbjct: 145 PQEPGIVPPLLDRVTPVHSIVPVDIYLPGCPPSATRIRAALEPLLQGKTP 194
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.323 0.144 0.444
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 92302299
Number of Sequences: 2977
Number of extensions: 3886708
Number of successful extensions: 8103
Number of sequences better than 1.0e-10: 18
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 8068
Number of HSP's gapped (non-prelim): 18
length of query: 241
length of database: 189,106,746
effective HSP length: 50
effective length of query: 191
effective length of database: 159,182,396
effective search space: 30403837636
effective search space used: 30403837636
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 164 (68.3 bits)