BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0641 (hydA-2) DE:CYTOCHROME-C3 HYDROGENASE ALPHA CHAIN
(415 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||B75070 cytochrome-c3 hydrogenase alpha chain PAB0641 - Pyro... 824 0.0
gb|AAF61854.1|AF176650_4 (AF176650) sulfhydrogenase II subunit a... 645 0.0
pir||S48836 cytochrome-c3 hydrogenase (EC 1.12.2.1) alpha chain ... 298 8e-80
pir||B75133 cytochrome-c3 hydrogenase alpha chain PAB1787 - Pyro... 298 1e-79
pir||C71075 probable cytochrome-c3 hydrogenase alpha chain - Pyr... 296 4e-79
gb|AAB94936.1| (AF039208) NiFe hydrogenase alpha subunit [Thermo... 290 2e-77
emb|CAA56464.1| (X80180) hydrogenase large alpha subunit [Acetom... 196 3e-49
pir||G64448 methylviologen-reducing hydrogenase alpha chain homo... 182 7e-45
gi|11498968 methylviologen-reducing hydrogenase, subunit alpha (... 181 2e-44
gb|AAB57892.1| (U70364) NAD-reducing hydrogenase beta subunit [R... 168 1e-40
pir||B37777 methyl viologen-reducing hydrogenase large chain - M... 161 1e-38
sp|P22320|HOXH_ALCEU NAD-REDUCING HYDROGENASE HOXS BETA SUBUNIT ... 157 3e-37
pir||A59304 methylviologen-reducing hydrogenase alpha chain vhuA... 152 7e-36
pir||S24801 methylviologen-reducing hydrogenase chain vhuA - Met... 150 3e-35
pir||S16727 coenzyme F420 hydrogenase (EC 1.12.99.1) alpha chain... 147 2e-34
pir||S72553 hydrogenase large chain - Anabaena variabilis >gi|21... 147 2e-34
pir||E30315 methyl viologen-reducing hydrogenase alpha chain - M... 145 1e-33
pir||A69018 probable coenzyme F420 hydrogenase (EC 1.12.99.1) al... 144 2e-33
pir||A70370 hydrogenase large subunit - Aquifex aeolicus >gi|298... 139 5e-32
pir||S74233 bidirectional hydrogenase chain H - Synechococcus sp... 136 5e-31
pir||S75530 hydrogenase large chain - Synechocystis sp. (strain ... 130 3e-29
gb|AAB53705.1| (U88400) bidirectional hydrogenase large subunit ... 127 3e-28
gb|AAB85780.1| (AE000895) coenzyme F420-reducing hydrogenase, al... 120 3e-26
gb|AAD38065.1|AF153438_1 (AF153438) HoxH [Rhodobacter capsulatus] 120 3e-26
sp|P19496|FRHA_METTH COENZYME F420 HYDROGENASE ALPHA SUBUNIT (8-... 120 3e-26
emb|CAA43500.1| (X61202) F420-reducing hydrogenase subunit [Meth... 119 7e-26
sp|Q60338|FRHA_METJA COENZYME F420 HYDROGENASE ALPHA SUBUNIT (8-... 113 4e-24
sp|Q00390|FRHA_METVO COENZYME F420 HYDROGENASE ALPHA SUBUNIT (8-... 113 5e-24
pir||HQDVLB cytochrome-c3 hydrogenase (EC 1.12.2.1) (NiFeSe) lar... 107 2e-22
sp|P13065|PHSL_DESBA PERIPLASMIC [NIFESE] HYDROGENASE LARGE SUBU... 107 2e-22
pdb|1CC1|L Chain L, Crystal Structure Of A Reduced, Active Form ... 101 2e-20
emb|CAA74094.1| (Y13764) F420-reducing hydrogenase isoenzyme I a... 98 2e-19
gb|AAA23375.2| (M18271) large hydrogenase subunit [Desulfomicrob... 95 2e-18
pir||F30315 methyl viologen-reducing hydrogenase (EC 1.-.-.-) al... 77 3e-13
sp|P18188|PHNL_DESFR PERIPLASMIC [NIFE] HYDROGENASE LARGE SUBUNI... 74 3e-12
pdb|1FRF|L Chain L, Crystal Structure Of The Ni-Fe Hydrogenase F... 74 3e-12
sp|P21852|PHNL_DESVM PERIPLASMIC [NIFE] HYDROGENASE LARGE SUBUNI... 72 1e-11
pdb|1H2R|L Chain L, Three-Dimensional Structure Of Ni-Fe Hydroge... 70 5e-11
>pir||B75070 cytochrome-c3 hydrogenase alpha chain PAB0641 - Pyrococcus abyssi
(strain Orsay) >gi|5458375|emb|CAB49863.1| (AJ248286)
CYTOCHROME-C3 HYDROGENASE ALPHA CHAIN [Pyrococcus
abyssi]
Length = 415
Score = 824 bits (2106), Expect = 0.0
Identities = 415/415 (100%), Positives = 415/415 (100%)
Query: 1 MVVMIIELDEFTRVEGIGKAEIVIENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEA 60
MVVMIIELDEFTRVEGIGKAEIVIENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEA
Sbjct: 1 MVVMIIELDEFTRVEGIGKAEIVIENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEA 60
Query: 61 RICAICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYG 120
RICAICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYG
Sbjct: 61 RICAICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYG 120
Query: 121 YPDAISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELEKIA 180
YPDAISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELEKIA
Sbjct: 121 YPDAISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELEKIA 180
Query: 181 KHARALVRFARRIVNIFATQEPRGAKIEVPMVTSEYLWGDELIAGNDRIQYEDIEEVPVP 240
KHARALVRFARRIVNIFATQEPRGAKIEVPMVTSEYLWGDELIAGNDRIQYEDIEEVPVP
Sbjct: 181 KHARALVRFARRIVNIFATQEPRGAKIEVPMVTSEYLWGDELIAGNDRIQYEDIEEVPVP 240
Query: 241 YSFAKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIKSGHVIYNNLAQAIEL 300
YSFAKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIKSGHVIYNNLAQAIEL
Sbjct: 241 YSFAKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIKSGHVIYNNLAQAIEL 300
Query: 301 VYALEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGVLVHHYRIENERIVWSNT 360
VYALEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGVLVHHYRIENERIVWSNT
Sbjct: 301 VYALEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGVLVHHYRIENERIVWSNT 360
Query: 361 ITPTAFNQGMMEKSLLEDARNMFGKESETTLKSKLEEVVRAFDPCISCSVHVVRL 415
ITPTAFNQGMMEKSLLEDARNMFGKESETTLKSKLEEVVRAFDPCISCSVHVVRL
Sbjct: 361 ITPTAFNQGMMEKSLLEDARNMFGKESETTLKSKLEEVVRAFDPCISCSVHVVRL 415
>gb|AAF61854.1|AF176650_4 (AF176650) sulfhydrogenase II subunit a [Pyrococcus furiosus]
Length = 412
Score = 645 bits (1645), Expect = 0.0
Identities = 309/412 (75%), Positives = 362/412 (87%)
Query: 4 MIIELDEFTRVEGIGKAEIVIENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARIC 63
MIIELDEFTRVEG GKAEIVIENG VKDARVKI+EGPRFFE+LTLGR Y DVPDLEARIC
Sbjct: 1 MIIELDEFTRVEGNGKAEIVIENGEVKDARVKIVEGPRFFEILTLGRDYWDVPDLEARIC 60
Query: 64 AICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPD 123
AICY +HS+ASVRAIE ALGI+V ESVEKLRELALWGEIIESHALHLYLLALPDV+GYPD
Sbjct: 61 AICYIAHSVASVRAIEKALGIDVPESVEKLRELALWGEIIESHALHLYLLALPDVFGYPD 120
Query: 124 AISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELEKIAKHA 183
AISM+ ++GEL+KEGLTIKA GN IRE I GREIHGIN+KPGGFGRYPSE ELEKIA+H+
Sbjct: 121 AISMIPRHGELVKEGLTIKAFGNAIRELIGGREIHGINIKPGGFGRYPSEEELEKIAEHS 180
Query: 184 RALVRFARRIVNIFATQEPRGAKIEVPMVTSEYLWGDELIAGNDRIQYEDIEEVPVPYSF 243
++L++FARRIV IFA+QE GA EV M TS+YLWGDELI +R+QY +++EVPV YSF
Sbjct: 181 KSLIKFARRIVGIFASQEAGGAVGEVLMATSDYLWGDELIINGERVQYYEVDEVPVGYSF 240
Query: 244 AKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIKSGHVIYNNLAQAIELVYA 303
AKHSYYKG P+FVGAL RLLLKG +EGEA R+L+ Y++K++S +VIYNNLAQAIEL+YA
Sbjct: 241 AKHSYYKGNPVFVGALPRLLLKGESIEGEAARMLEEYRDKLESKYVIYNNLAQAIELLYA 300
Query: 304 LEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGVLVHHYRIENERIVWSNTITP 363
LE ++ EE++SEGIE+ E S++SGEG+GYVEAPRGVLVHHYRIEN ++VWSNTITP
Sbjct: 301 LERVPQLVEEILSEGIERGNGEISQESGEGVGYVEAPRGVLVHHYRIENGKVVWSNTITP 360
Query: 364 TAFNQGMMEKSLLEDARNMFGKESETTLKSKLEEVVRAFDPCISCSVHVVRL 415
TAFNQ +ME SLLE+A+ ++G ESE +K +LE +VRAFDPCISCSVH V+L
Sbjct: 361 TAFNQRLMELSLLEEAKRLYGSESEENMKKRLEVIVRAFDPCISCSVHFVKL 412
>pir||S48836 cytochrome-c3 hydrogenase (EC 1.12.2.1) alpha chain - Pyrococcus
furiosus >gi|562777|emb|CAA53037.1| (X75255) hydrogenase
(alpha subunit) [Pyrococcus furiosus]
Length = 428
Score = 298 bits (755), Expect = 8e-80
Identities = 168/423 (39%), Positives = 251/423 (58%), Gaps = 15/423 (3%)
Query: 6 IELDEFTRVEGIGKAEIVIENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICAI 65
I +D RVEG G EI+I + VK+ ++ I+EGPRFFE +T+G+ + + RIC+
Sbjct: 8 ITIDHIARVEGKGGVEIIIGDDGVKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICSF 67
Query: 66 CYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPDAI 125
C A+H + ++ A E A+G E ++ LRE+ G++IESHALHLYLL LPD GY +
Sbjct: 68 CSAAHKLTALEAAEKAVGFVPREEIQALREVLYIGDMIESHALHLYLLVLPDYRGYSSPL 127
Query: 126 SMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELEKIAKHARA 185
MV++Y I+ L +K LG + + + R IH N GGFG+ P + LEK+ R
Sbjct: 128 KMVNEYKREIEIALKLKNLGTWMMDILGSRAIHQENAVLGGFGKLPEKSVLEKMKAELRE 187
Query: 186 LVRFARRIVNIFATQEPRGAKIEVPMV--------TSEYLWGDELIAGNDR----IQYED 233
+ A +FA E + +++E P+ + ++GD + A + +Y D
Sbjct: 188 ALPLAEYTFELFAKLE-QYSEVEGPITHLAVKPRGDAYGIYGDYIKASDGEEFPSEKYRD 246
Query: 234 -IEEVPVPYSFAKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIKSGHVIYN 292
I+E V +SFAKHS+YKG+P VGA++R++ L G+AK L ++ K+ +K + N
Sbjct: 247 YIKEFVVEHSFAKHSHYKGRPFMVGAISRVINNADLLYGKAKELYEANKDLLKGTNPFAN 306
Query: 293 NLAQAIELVYALEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGVLVHHYRIEN 352
NLAQA+E+VY +E A + +E +++ K E K G G+ EAPRG+LV+ ++EN
Sbjct: 307 NLAQALEIVYFIERAIDLLDEALAKWPIKPRDEVEIKDGFGVSTTEAPRGILVYALKVEN 366
Query: 353 ERIVWSNTITPTAFNQGMMEKSLLEDARNMFGKESETTLKSKLEEVVRAFDPCISCSVHV 412
R+ +++ ITPTAFN MME+ + A + + E LK E VVRA+DPCISCSVHV
Sbjct: 367 GRVSYADIITPTAFNLAMMEEHVRMMAEKHYNDDPE-RLKILAEMVVRAYDPCISCSVHV 425
Query: 413 VRL 415
VRL
Sbjct: 426 VRL 428
>pir||B75133 cytochrome-c3 hydrogenase alpha chain PAB1787 - Pyrococcus abyssi
(strain Orsay) >gi|5458290|emb|CAB49779.1| (AJ248285)
CYTOCHROME-C3 HYDROGENASE ALPHA CHAIN [Pyrococcus
abyssi]
Length = 428
Score = 298 bits (754), Expect = 1e-79
Identities = 166/425 (39%), Positives = 246/425 (57%), Gaps = 19/425 (4%)
Query: 6 IELDEFTRVEGIGKAEIVIENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICAI 65
I +D RVEG G EI++ + VK+ ++ I+EGPRFFE +T+G+ + + RIC+
Sbjct: 8 ITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICSF 67
Query: 66 CYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPDAI 125
C A+H + ++ A E A+G E ++ LRE+ G++IESHALHLYLL LPD GY +
Sbjct: 68 CSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGYSSPL 127
Query: 126 SMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELEKIAKHARA 185
MV++Y + ++ L +K LG+ + + + R IH N GGFG+ PS+ LE++ R
Sbjct: 128 KMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEEMKAKLRE 187
Query: 186 LVRFARRIVNIFATQE---------------PRGAKIEVPMVTSEYLWGDELIAGNDRIQ 230
+ A +FA E PRG +V + +Y+ +
Sbjct: 188 SLSLAEYTFELFAKLEQYREVEGEITHLAVKPRG---DVYGIYGDYIKASDGEEFPSEDY 244
Query: 231 YEDIEEVPVPYSFAKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIKSGHVI 290
E I E V +SFAKHS+YKGKP VGA++R++ L G AK L +S+KE +K +
Sbjct: 245 KEHINEFVVEHSFAKHSHYKGKPFMVGAISRVVNNKDLLYGRAKDLYESHKELLKGTNPF 304
Query: 291 YNNLAQAIELVYALEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGVLVHHYRI 350
NNLAQA+ELVY +E A + +E++ + K + + G G+ EAPRG+LV+ ++
Sbjct: 305 ANNLAQALELVYFIERAIDLIDEVLIKWPVKERDKVEVRDGFGVSTTEAPRGILVYALKV 364
Query: 351 ENERIVWSNTITPTAFNQGMMEKSLLEDARNMFGKESETTLKSKLEEVVRAFDPCISCSV 410
EN R+ +++ ITPTAFN MME+ + A + + E LK E VVRA+DPCISCSV
Sbjct: 365 ENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDDPE-RLKLLAEMVVRAYDPCISCSV 423
Query: 411 HVVRL 415
HVV+L
Sbjct: 424 HVVKL 428
>pir||C71075 probable cytochrome-c3 hydrogenase alpha chain - Pyrococcus
horikoshii >gi|3257714|dbj|BAA30397.1| (AP000005) 429aa
long hypothetical cytochrome-c3 hydrogenase alpha chain
[Pyrococcus horikoshii]
Length = 429
Score = 296 bits (749), Expect = 4e-79
Identities = 163/422 (38%), Positives = 252/422 (59%), Gaps = 13/422 (3%)
Query: 6 IELDEFTRVEGIGKAEIVIENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICAI 65
I +D R+EG EI++ VK+ ++ I+EGPRFFE +TLG+ + + RIC+
Sbjct: 8 ITVDHIARIEGKAGVEILVGEDGVKEVKLNIIEGPRFFEAITLGKKLEEALAIYPRICSF 67
Query: 66 CYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPDAI 125
C A+H + ++ A E A+G E ++ LRE+ G+IIESHALHLYLL LPD GY +
Sbjct: 68 CSAAHKLTALEAAEKAIGFTPREEIQALREILYIGDIIESHALHLYLLVLPDYLGYSSPL 127
Query: 126 SMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELEKIAKHARA 185
MV +Y + ++ + +K LG+ I + + R IH N GGFG+ PS+ LEKI ++
Sbjct: 128 KMVDEYKKELETAIKLKNLGSWIMDVLGARAIHQENAILGGFGKLPSKETLEKIKDELKS 187
Query: 186 LVRFARRIVNIFATQEP-RGAKIEV------PMVTSEYLWGDELIAGNDR----IQYED- 233
+ A +F+ E + + E+ P + ++GD + A + +Y++
Sbjct: 188 ALPLAEYTFELFSKLEQYKEVEGEITHLAVKPRKDAYGIYGDRIKASDGEEFPSEEYKNY 247
Query: 234 IEEVPVPYSFAKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIKSGHVIYNN 293
I+E V +SFAKHS+YKG+P VGA++RL+ L G+AK L ++ K+ ++ + NN
Sbjct: 248 IKEFVVEHSFAKHSHYKGRPFMVGAISRLVNNHKLLYGKAKELYENNKDLLRPTNPFANN 307
Query: 294 LAQAIELVYALEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGVLVHHYRIENE 353
LAQA+E+VY +E A + +E++++ K E + G G+ EAPRG+LV+ ++EN
Sbjct: 308 LAQALEIVYFMERAIDLIDEVLAKWPIKPRDEVKVRDGFGVSTTEAPRGILVYALKVENG 367
Query: 354 RIVWSNTITPTAFNQGMMEKSLLEDARNMFGKESETTLKSKLEEVVRAFDPCISCSVHVV 413
R+ +++ ITPTAFN MME+ + A + K+ LK E VVRA+DPCISCSVHVV
Sbjct: 368 RVSYADIITPTAFNLAMMERHVRMMAEEHY-KDDPEKLKLLAEMVVRAYDPCISCSVHVV 426
Query: 414 RL 415
+L
Sbjct: 427 KL 428
>gb|AAB94936.1| (AF039208) NiFe hydrogenase alpha subunit [Thermococcus litoralis]
Length = 426
Score = 290 bits (734), Expect = 2e-77
Identities = 162/423 (38%), Positives = 244/423 (57%), Gaps = 14/423 (3%)
Query: 6 IELDEFTRVEGIGKAEIVIENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICAI 65
I +D RVEG G EIV + VK+ ++ I+EGPRFFE +T+G+ + + R+C+
Sbjct: 5 ITVDHIARVEGKGGIEIVTSDEGVKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRVCSF 64
Query: 66 CYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPDAI 125
C ASH + ++ A E A+G ++ LREL G+ IESHALHLYLL LPD GY + +
Sbjct: 65 CSASHKLTALEAAEKAIGFTPRPEIQDLRELLYMGDTIESHALHLYLLVLPDYLGYSNPL 124
Query: 126 SMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELEKIAKHARA 185
+MV KY + I+ + +K +G+ I + + R IH NV GGFG+ P++ + E++ + +
Sbjct: 125 AMVDKYKKEIEYAMALKNVGSKIMDYLGSRAIHQENVVLGGFGKLPTKAQFEELRRELKE 184
Query: 186 LVRFARRIVNIFATQEPRGAKIEVPMVTSEY--------LWGDEL-IAGNDRIQYED--- 233
+ A V +F+ E + MV ++GD + ++ ED
Sbjct: 185 ALPLAEYTVELFSKLEQYEEVTDDEMVHMAVKPRNDVYGIYGDYIKVSDGFEFPVEDYKK 244
Query: 234 -IEEVPVPYSFAKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIKSGHVIYN 292
I E V +SFAKHS+YKGKP VGA++R++ L G+AK L YK+ ++ + N
Sbjct: 245 HIVEKVVEHSFAKHSFYKGKPFMVGAISRIVNNADLLYGKAKELYTQYKDLLRYNNCFAN 304
Query: 293 NLAQAIELVYALEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGVLVHHYRIEN 352
N AQAIELVY +E + I ++ +++ K E K G G+ EAPRG+LV+ +++
Sbjct: 305 NFAQAIELVYFIEKSIDIIDDTLAKWPVKERDEVELKDGFGVSITEAPRGLLVYALEVKD 364
Query: 353 ERIVWSNTITPTAFNQGMMEKSLLEDARNMFGKESETTLKSKLEEVVRAFDPCISCSVHV 412
R+ +++ ITPTA N +ME+ + A N + + E LK E VRA+DPCISCSVHV
Sbjct: 365 GRVNYADIITPTAMNLAIMERHVRMMAENHWQDDPE-RLKLLAEMTVRAYDPCISCSVHV 423
Query: 413 VRL 415
VRL
Sbjct: 424 VRL 426
>emb|CAA56464.1| (X80180) hydrogenase large alpha subunit [Acetomicrobium flavidum]
Length = 475
Score = 196 bits (494), Expect = 3e-49
Identities = 146/453 (32%), Positives = 227/453 (49%), Gaps = 46/453 (10%)
Query: 3 VMIIELDEFTRVEGIGKAEIVI-ENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEAR 61
V +E++ TR+EG GK +++ E+G V++ R + + R FEV T GR + ++P + R
Sbjct: 4 VFKLEINPVTRIEGHGKITVMLDESGHVRETRFHVTQY-RGFEVFTHGRDFREMPVITPR 62
Query: 62 ICAICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGY 121
IC IC SH +AS +A + LG+ ++ + KLREL G+I++SHAL + L+ PD+
Sbjct: 63 ICGICPVSHHLASAKACDEILGVTITPAAHKLRELMHMGQIVQSHALSFFHLSSPDILLG 122
Query: 122 PDA-------ISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSER 174
DA +V +Y EL K+G+ ++ G I +T+ G++IH + PGG R + +
Sbjct: 123 FDAPVKIRNVAGLVDRYPELAKKGIMLRKFGQEIIKTLGGKKIHPWHSIPGGVNRSLTPQ 182
Query: 175 ELEKIAKHARALVRFARRIVNIFATQEPRGAK--IEVPMVTSEY--LWGD---ELIAGND 227
E + IA + A + + G + E P + + Y L D EL G
Sbjct: 183 ERDAIAAQLPEMKSIAMEAIKLIKDYLQEGGEELKEFPTLDTAYMGLVRDGYLELYDGEV 242
Query: 228 RIQ---------------YEDIEEVPVPYSFAKHSYYK--GKP---IFVGALARLLLKGS 267
RI+ + I E P+S+ K +YK G P VG LARL +
Sbjct: 243 RIKAPRGRILDQFDPKDYLDHIGEHVEPWSYLKFPFYKALGFPHGSYRVGPLARLNAADA 302
Query: 268 KLEGEAKRLLDSYKEKIKSG---HVIYNNLAQAIELVYALEVASKIAEELISEGIEKSTC 324
EA + YKE + G + +Y + A+ IE +Y LE +I + L I S
Sbjct: 303 VSTPEASKEFALYKEMGEDGIVPYTLYYHYARLIEALYGLE---RIEQLLADPDITSSDL 359
Query: 325 EPSEK--SGEGIGYVEAPRGVLVHHYRIENERIVWS-NTITPTAFNQGMMEKSLLEDARN 381
+ K + EGIG +EAPRG L+HHY++ ++ N I T N M K + A+
Sbjct: 360 RVTSKEINPEGIGVIEAPRGTLIHHYQVNESGVITKVNLIVATGHNNFAMNKGVEMVAKK 419
Query: 382 -MFGKESETTLKSKLEEVVRAFDPCISCSVHVV 413
+ G + ++LE V+RA+DPC+SCS H V
Sbjct: 420 YITGTNVPEGVFNRLEHVIRAYDPCLSCSTHAV 452
>pir||G64448 methylviologen-reducing hydrogenase alpha chain homolog -
Methanococcus jannaschii >gi|1591820|gb|AAB99194.1|
(U67560) methylviologen-reducing hydrogenase, alpha
chain (vhuA) [Methanococcus jannaschii]
Length = 418
Score = 182 bits (457), Expect = 7e-45
Identities = 137/419 (32%), Positives = 214/419 (50%), Gaps = 46/419 (10%)
Query: 6 IELDEFTRVEGIGKAEIVI-ENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICA 64
I ++ +R+EG GK I + ENG KD ++ I R FE +GR +VP + RIC
Sbjct: 4 IVIEPLSRLEGHGKVTITLDENGKPKDVKLHIT-ALRGFEQFVVGRPAEEVPRIVPRICG 62
Query: 65 ICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPDA 124
IC +H +ASV+AI+ A G+E+ E +KLREL G +I SHALH Y LA PD PDA
Sbjct: 63 ICQTAHHLASVKAIDAAWGVEIPEPAKKLRELMHIGNMIHSHALHFYFLAAPDFVLGPDA 122
Query: 125 -------ISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELE 177
+ ++ K ++ K+ + ++ G I E + G+ IH + PGG + +E E +
Sbjct: 123 DPAIRNIVGVIDKAPDVAKQAIALRKFGQKIVEAVGGKAIHPVTGIPGGQAKRLTEEERD 182
Query: 178 KIAKHARALVRFARRIVNIF-ATQEPRGAKIEVPMVTSEYLWG----------DELIA-- 224
++ K A ++ +A+ V + E +I+ V Y G D+ +
Sbjct: 183 ELLKDADQMIEYAKNGVELIKQLNEQYMEQIKTLGVIDTYYLGLVKDGKHNFYDDTLRFL 242
Query: 225 ---GNDRIQYED------IEEVPVPYSFAKHSYYK--GKPIFVGALARLLLKGSKLEGEA 273
G ++++++ I E VPY++ KH YYK G P V + L + E E
Sbjct: 243 SPDGKEKVEFKPEEYLNYIGEYVVPYNYVKHPYYKKVGYPEGVYRVGPLAMLNVCDEMET 302
Query: 274 KRLLDSYKEK------IKSGHVIYNNLAQAIELVYALEVASKIAE--ELISEGIEKSTCE 325
L + Y+++ + + N A+ IELV A E A + E ++ S+ I K+ E
Sbjct: 303 P-LAEEYRKEFLEIFGFPANQSLAYNHARLIELVEACEKAKILLEDNDITSDDI-KADVE 360
Query: 326 PSEKSGEGIGYVEAPRGVLVHHYRI-ENERIVWSNTITPTAFNQGMMEKSLLEDARNMF 383
P K+G G+G V APRGVL+H+Y EN +V +N I T N MEK++ + A+ +F
Sbjct: 361 P--KAGNGVGVVYAPRGVLIHNYETDENGIVVKANMIVATTHNVPTMEKAIQQAAQVIF 417
>gi|11498968 methylviologen-reducing hydrogenase, subunit alpha (vhuA)
[Archaeoglobus fulgidus] >gi|7447185|pir||C69421
methylviologen-reducing hydrogenase, subunit alpha
(vhuA) homolog - Archaeoglobus fulgidus
>gi|2649200|gb|AAB89872.1| (AE001009)
methylviologen-reducing hydrogenase, subunit alpha
(vhuA) [Archaeoglobus fulgidus]
Length = 458
Score = 181 bits (454), Expect = 2e-44
Identities = 132/439 (30%), Positives = 225/439 (51%), Gaps = 46/439 (10%)
Query: 4 MIIELDEFTRVEGIGKAEIVIEN-GTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARI 62
M IE++ +R+EG K I +++ G V++A + E R +E + G +VP + + I
Sbjct: 1 MKIEINPVSRIEGHAKVTIFLDSSGKVENAFFQATEF-RGYEKILSGLPMEEVPRVVSTI 59
Query: 63 CAICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYP 122
C IC A H A+++A + G+E S++ + +RE+ L IE H L L LALPD
Sbjct: 60 CGICRAIHFTAALKAADQVFGVEASDAAKIVREMLLCAHFIEDHTLSLLALALPDFLSPE 119
Query: 123 D--AISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELEKIA 180
+ ++ GEL +E L + I + + GR IH + PGG+ + P++ EL I
Sbjct: 120 ERNIFGVIRNLGELGRELLRKRGYAVRILQILGGRNIHPVTALPGGWAKAPTDEELGLIE 179
Query: 181 KHARALVRFARRIVNIFATQEPRGAKIEVPMVT-------------SEYL----WGDELI 223
+++R L+ R+V + G +E+ T E + G E++
Sbjct: 180 RYSRELLDLGYRMVEVLEAALDMGDGLELRAATLGTEKKGLVNLYDGEQVVKDEEGKEVL 239
Query: 224 AGNDRIQYEDIEEVPVPYSFAKHSYYK--GKPIF-VGALARLLLKGSKLEGEAKRLLDSY 280
+ + + I E + +S++K + K G+ ++ VG LAR LK ++L +A ++ +Y
Sbjct: 240 RFSGKEYADIITEKSLSWSYSKLTEIKPLGEKLYVVGPLAR--LKTAELTPKASEIVKTY 297
Query: 281 KEKIKSGHVIYNNLAQAIELVYALEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAP 340
S +++YN+ A+A+E++YA E ++++ I+ EP + +GEG+G VEAP
Sbjct: 298 G---VSNNLLYNHFARAVEIIYAAERLLELSQSYRGGRIQN---EPQKVAGEGVGIVEAP 351
Query: 341 RGVLVHHYRIENERIV-WSNTITPTAFNQGMMEKSLLEDARNMFGKESE-----TTLKSK 394
RG L+HHY + + IV SN I T N + ++ GK +E L K
Sbjct: 352 RGTLIHHYWTDEKGIVRGSNLIVATTHN--------VRAINHVVGKVAERASDTRNLAEK 403
Query: 395 LEEVVRAFDPCISCSVHVV 413
+E +RAFDPC++C+ H V
Sbjct: 404 VEVAIRAFDPCMACATHAV 422
>gb|AAB57892.1| (U70364) NAD-reducing hydrogenase beta subunit [Rhodococcus opacus]
Length = 488
Score = 168 bits (420), Expect = 1e-40
Identities = 127/464 (27%), Positives = 223/464 (47%), Gaps = 62/464 (13%)
Query: 8 LDEFTRVEGIGKAEIVIE-NGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICAIC 66
+D TR+EG GK + ++ N V DA + ++E R FE L G + + P L RIC IC
Sbjct: 7 IDPVTRIEGHGKVTVHLDDNNNVVDAHLHVVEF-RGFEKLVQGHPFWEAPMLMQRICGIC 65
Query: 67 YASHSIASVRAIENAL------GIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYG 120
+ SH + +A+++ + GI+V+ + EK+R L + ++++SHA + L +P++
Sbjct: 66 FVSHHLCGAKALDDMVGVGLKSGIDVTPTAEKIRRLGHYAQMLQSHATAYFYLIVPEMLF 125
Query: 121 YPDA-------ISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSE 173
DA + ++ EL+K + ++ G + + + GR +HGI+ PGG + S
Sbjct: 126 GMDAAPEQRNVLGLIEANPELVKRVVMLRKWGQEVIKAVFGRRMHGISSVPGGVNKNLSV 185
Query: 174 RELEKIAKHARAL------VRFARRIVNIFATQEPRG----------AKIEVPMVTS--- 214
E ++ K L + +A+ V +F + + + + +V +
Sbjct: 186 AECQRFLKGEEGLPSVDEVIEYAQEGVQLFYDFHEQNRVQVDSFANVSALSMSLVDADGN 245
Query: 215 -EYLWGDELIAGNDR-----IQYED----IEEVPVPYSFAKHSYYKG-----KPIFVGAL 259
+Y G I +D+ Y D E +S+ K + K + VG L
Sbjct: 246 VDYYHGKLRIIDDDKNVVQEFDYHDYLDHFSEAVEEWSYMKFPFLKALGRERGSVRVGPL 305
Query: 260 ARLLLKGS---KLEGEAKRLLDSYKEKIKSGHVIYNNLAQAIELVYALEVASKIAEELIS 316
RL + S L EA +Y + ++ N A+AIE+++A E+ I E L
Sbjct: 306 GRLNVTNSLSTPLAQEALERFHAYTNGKANNMTLHTNWARAIEILHAAEL---IKELLND 362
Query: 317 EGIEKS----TCEPSEKSGEGIGYVEAPRGVLVHHYRIENE-RIVWSNTITPTAFNQGMM 371
++K T + +GEG+G VEAPRG L+HHYR + E I ++N + T N +M
Sbjct: 363 PDLQKEQLLLTPADNAWTGEGVGVVEAPRGTLLHHYRADQEGDITFANLVVATTQNNQVM 422
Query: 372 EKSLLEDARNMFGKESETT--LKSKLEEVVRAFDPCISCSVHVV 413
+++ A + G + E T + + +E +RA+DPC+SC+ H +
Sbjct: 423 NRTVRSVAEDYLGGQGEVTEGMMNAIEVGIRAYDPCLSCATHAL 466
>pir||B37777 methyl viologen-reducing hydrogenase large chain - Methanothermus
fervidus >gi|149805|gb|AAA72832.1| (M34016) methyl
viologen-reducing hydrogenase large subunit (mvhA)
[Methanothermus fervidus]
Length = 472
Score = 161 bits (403), Expect = 1e-38
Identities = 131/449 (29%), Positives = 206/449 (45%), Gaps = 47/449 (10%)
Query: 8 LDEFTRVEGIGKAEIVI-ENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICAIC 66
L+ TR+EG K + + E G VKD R ++E R FE GR + P + RIC IC
Sbjct: 6 LEPVTRIEGHAKITVQLDEEGNVKDTRFHVMEF-RGFEKFLQGRRIEEAPRIVPRICGIC 64
Query: 67 YASHSIASVRAIENALGIE---VSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPD 123
H +AS +A++ G E + E+ K+RE+ W + SH LH Y LA PD G D
Sbjct: 65 QVQHHLASAKAVDACFGFEPEDIPETAYKMREIMNWASYVHSHGLHFYFLAAPDFIGGKD 124
Query: 124 -----AISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELEK 178
++ ++ K+ + ++ I GR IH ++ PGG + EK
Sbjct: 125 RETRNIFKIIQDSPDVAKQAIELRKNAQDIVAATGGRAIHPVSFIPGGITTELDKETQEK 184
Query: 179 IAKHARALVRFARRIVNI-FATQEPRGAKIEVPMVTSEYLWG----------DELIAGND 227
+ K A+ V A + + E +E Y G D ++ D
Sbjct: 185 LLKKAKRNVEIAESTLELAIPIFEDNMDLVESLGTIETYHMGLVKNGTWDVYDGVVRVKD 244
Query: 228 R----------IQY-EDIEEVPVPYSFAKHSYYK--GKP---IFVGALARLLLKGSKLEG 271
+ +Y + I E PYS+ K Y K G P V L+RL + K+
Sbjct: 245 KNGENFAEFGPDEYTKYIAEHVKPYSWLKFPYLKELGYPDGVYRVSPLSRLNV-ADKMPD 303
Query: 272 EAKRLLDSYKE-KIKSGHV---IYNNLAQAIELVYALEVASKIAEELISEGIEKSTCEPS 327
A + + +KE + K G+ + + A+ IE++ A E + + EE +S +K +
Sbjct: 304 AAPKAQEYFKEFRNKFGYAQQPLLYHWARLIEMLAAAECMASVLEEDLSG--KKIHGKLE 361
Query: 328 EKSGEGIGYVEAPRGVLVHHYRIENERIVW-SNTITPTAFNQGMMEKSLLEDARNMF--G 384
+ GEG+G VEAPRG L+HHY + I+ +N I T N ME + + A+ G
Sbjct: 362 RQEGEGVGIVEAPRGTLIHHYACDKNGIITKANLIVATVQNNPAMEMGIQKVAKERIKPG 421
Query: 385 KESETTLKSKLEEVVRAFDPCISCSVHVV 413
+ + + + +E V+RA+DPC+SC+ H V
Sbjct: 422 VDVDDKIYNLMEMVIRAYDPCLSCATHTV 450
>sp|P22320|HOXH_ALCEU NAD-REDUCING HYDROGENASE HOXS BETA SUBUNIT >gi|95477|pir||D35385
hydrogen dehydrogenase (EC 1.12.1.2) beta chain,
cytosolic - Alcaligenes eutrophus
>gi|141950|gb|AAC06143.1| (M55230) NAD-reducing
hydrogenase [Ralstonia eutropha]
Length = 488
Score = 157 bits (392), Expect = 3e-37
Identities = 124/464 (26%), Positives = 223/464 (47%), Gaps = 62/464 (13%)
Query: 8 LDEFTRVEGIGKAEIVIENGT-VKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICAIC 66
+D TR+EG GK + +++ V DA++ ++E R FE G + + P RIC IC
Sbjct: 7 IDPVTRIEGHGKVVVHLDDDNKVVDAKLHVVEF-RGFEKFVQGHPFWEAPMFLQRICGIC 65
Query: 67 YASHSIASVRAIENALG------IEVSESVEKLRELALWGEIIESHALHLYLLALPDVYG 120
+ SH + +A+++ +G I V+ + EK+R L + ++++SH + L +P++
Sbjct: 66 FVSHHLCGAKALDDMVGVGLKSGIHVTPTAEKMRRLGHYAQMLQSHTTAYFYLIVPEMLF 125
Query: 121 YPDA-------ISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSE 173
DA + ++ +L+K + ++ G + + + G+++HGIN PGG S
Sbjct: 126 GMDAPPAQRNVLGLIEANPDLVKRVVMLRKWGQEVIKAVFGKKMHGINSVPGGVNNNLSI 185
Query: 174 RELEKIAKHARAL------VRFARRIVNIFAT-QEPRGAKIE----VPMVTS-------- 214
E ++ L + +A+ + +F + A+++ VP ++
Sbjct: 186 AERDRFLNGEEGLLSVDQVIDYAQDGLRLFYDFHQKHRAQVDSFADVPALSMCLVGDDDN 245
Query: 215 -EYLWGDELIAGNDR-----IQYED----IEEVPVPYSFAKHSYYK--GKP---IFVGAL 259
+Y G I +D+ Y D E +S+ K Y K G+ + VG L
Sbjct: 246 VDYYHGRLRIIDDDKHIVREFDYHDYLDHFSEAVEEWSYMKFPYLKELGREQGSVRVGPL 305
Query: 260 ARLLLKGS---KLEGEAKRLLDSYKEKIKSGHVIYNNLAQAIELVYALEVASKIAEELIS 316
R+ + S L EA +Y + + ++ N A+AIE+++A EV + E L
Sbjct: 306 GRMNVTKSLPTPLAQEALERFHAYTKGRTNNMTLHTNWARAIEILHAAEV---VKELLHD 362
Query: 317 EGIEKS----TCEPSEKSGEGIGYVEAPRGVLVHHYRI-ENERIVWSNTITPTAFNQGMM 371
++K T P+ +GEG+G VEAPRG L+HHYR E I ++N + T N +M
Sbjct: 363 PDLQKDQLVLTPPPNAWTGEGVGVVEAPRGTLLHHYRADERGNITFANLVVATTQNHQVM 422
Query: 372 EKSLLEDARNMFGKESETT--LKSKLEEVVRAFDPCISCSVHVV 413
+++ A + G E T + + +E +RA+DPC+SC+ H +
Sbjct: 423 NRTVRSVAEDYLGGHGEITEGMMNAIEVGIRAYDPCLSCATHAL 466
>pir||A59304 methylviologen-reducing hydrogenase alpha chain vhuA [similarity] -
Methanococcus voltae >gi|1747408|emb|CAA43510.1|
(X61204) methylviologen-reducing hydrogenase subunit
[Methanococcus voltae]
Length = 420
Score = 152 bits (380), Expect = 7e-36
Identities = 123/418 (29%), Positives = 207/418 (49%), Gaps = 44/418 (10%)
Query: 6 IELDEFTRVEGIGKAEIVIENGTVKDARVKI-LEGPRFFEVLTLGRHYMDVPDLEARICA 64
I + TR+EG GK I +++ K A VK+ + R FE +GR +VP + RIC
Sbjct: 4 ITIAPLTRLEGHGKVTIKLDDSG-KPADVKLHITALRGFEQFVIGRPAEEVPRIVPRICG 62
Query: 65 ICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPDA 124
IC +H +ASV+A++ A G ++ + EK REL G +I SHALH Y LA PD PDA
Sbjct: 63 ICQTAHHLASVKAVDAAWGAQIPSAAEKQRELMHLGNMIHSHALHFYYLAAPDFVLGPDA 122
Query: 125 -------ISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELE 177
+ ++ E+ K+ + ++ +G + E G+ IH + PGG + SE + +
Sbjct: 123 DPAIRNIVGVIDAAPEVAKKAIAMRRVGQSMVEATGGKPIHPVTGIPGGLSKSMSEEKRD 182
Query: 178 KIAKHARALVRFARRIVNIFATQEPRGAKI--EVPMVTSEYL----------WGDEL--- 222
++ ++++ + +++ + + + ++ + YL +GD L
Sbjct: 183 ELLAEIDTMIQYGQDGLDLMKSLNEKYLDTINSLGVIDTWYLGLVKDGKHNFYGDTLRFV 242
Query: 223 -IAGNDRIQYEDIE------EVPVPYSFAKHSYYK--GKP---IFVGALARLLLKGSKLE 270
G+++++++ E E V +S+ K+ Y K G P VG LA + + S
Sbjct: 243 SPDGSEKMEFKPAEYLDYLGEHVVEHSYVKYPYNKKVGYPEGLYRVGPLAMINVCDSMST 302
Query: 271 GEAKRLLDSYKEKI--KSGHVIYNNLAQAIELVYALEVASKIAE--ELISEGIEKSTCEP 326
A+ + E + I N A+ IEL+ A E A ++ E E++S + K+ EP
Sbjct: 303 PLAEEARKEFAETFGRPANQSIAYNQARLIELLSACERAKELLEDPEIVSTDV-KAEVEP 361
Query: 327 SEKSGEGIGYVEAPRGVLVHHYRIENERIVW-SNTITPTAFNQGMMEKSLLEDARNMF 383
K+G G+G V APRG L H+Y ++ IV +N I T N MEK++ + A +F
Sbjct: 362 --KAGNGVGVVYAPRGTLFHNYETDDNGIVTKANMIVATTHNVPTMEKAIQQAAEVLF 417
>pir||S24801 methylviologen-reducing hydrogenase chain vhuA - Methanococcus
voltae
Length = 420
Score = 150 bits (375), Expect = 3e-35
Identities = 123/418 (29%), Positives = 207/418 (49%), Gaps = 44/418 (10%)
Query: 6 IELDEFTRVEGIGKAEIVIENGTVKDARVKI-LEGPRFFEVLTLGRHYMDVPDLEARICA 64
I + TR+EG GK I +++ K A VK+ + R FE +GR +VP + RIC
Sbjct: 4 ITIAPLTRLEGHGKVTIKLDDSG-KPADVKLHITALRGFEQFVIGRPAEEVPRIVPRICG 62
Query: 65 ICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPDA 124
IC +H +ASV+A++ A G ++ + EK REL G +I SHALH Y LA PD PDA
Sbjct: 63 ICQTAHHLASVKAVDAAWGAQIPSAAEKQRELMHLGNMIHSHALHFYYLAAPDFVLGPDA 122
Query: 125 -------ISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELE 177
+ ++ E+ K+ + ++ +G + E G+ IH + PGG + SE + +
Sbjct: 123 DPAIRNIVGVIDARPEVRKKAIAMRRVGQSMVEATGGKPIHPVTGIPGGLSKSMSEEKRD 182
Query: 178 KIAKHARALVRFARRIVNIFATQEPRGAKI--EVPMVTSEYL----------WGDEL--- 222
++ ++++ + +++ + + + ++ + YL +GD L
Sbjct: 183 ELLAEIDTMIQYGQDGLDLMKSLNEKYLDTINSLGVIDTWYLGFVKDGKHNFYGDTLRFV 242
Query: 223 -IAGNDRIQYEDIE------EVPVPYSFAKHSYYK--GKP---IFVGALARLLLKGSKLE 270
G+++++++ E E V +S+ K+ Y K G P VG LA + + S
Sbjct: 243 SPDGSEKMEFKPAEYLDYLGEHVVEHSYVKYPYNKKEGYPEGLYRVGPLAMINVCDSMST 302
Query: 271 GEAKRLLDSYKEKI--KSGHVIYNNLAQAIELVYALEVASKIAE--ELISEGIEKSTCEP 326
A+ + E + I N A+ IEL+ A E A ++ E E++S + K+ EP
Sbjct: 303 PLAEEARKEFAETFGRPANQSIAYNQARLIELLSACERAKELLEDPEIVSTDV-KAEVEP 361
Query: 327 SEKSGEGIGYVEAPRGVLVHHYRIENERIVW-SNTITPTAFNQGMMEKSLLEDARNMF 383
K+G G+G V APRG L H+Y ++ IV +N I T N MEK++ + A +F
Sbjct: 362 --KAGIGVGVVYAPRGTLFHNYETDDNGIVTKANMIVATTHNVPTMEKAIQQAAEVLF 417
>pir||S16727 coenzyme F420 hydrogenase (EC 1.12.99.1) alpha chain vhcA
[similarity] - Methanococcus voltae
>gi|44794|emb|CAA43506.1| (X61203) vhcA [Methanococcus
voltae]
Length = 474
Score = 147 bits (368), Expect = 2e-34
Identities = 122/454 (26%), Positives = 216/454 (46%), Gaps = 50/454 (11%)
Query: 6 IELDEFTRVEGIGKAEIVIEN-GTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICA 64
+ ++ TRVEG GK + ++ G + ++E R FE GR+ D P RIC
Sbjct: 4 LSIEPVTRVEGHGKVTLSFDDSGKLDKVNFHVVE-VRGFEKFLEGRYIEDAPIFTPRICG 62
Query: 65 ICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYP-- 122
IC SH +AS +A++N G+++ E+ LR L + SHALH +LA PD+ +P
Sbjct: 63 ICQVSHHLASAKAVDNVFGVKIPETANMLRNLIHQASNVHSHALHFGMLASPDLM-FPTT 121
Query: 123 ------DAISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSEREL 176
+ + + + ++IK+ + ++ +G I + + GR IH + GG + +E E
Sbjct: 122 DDALKRNLLGVAGENMDMIKDSIAMRKVGQTIVQKVGGRAIHPVTAIVGGQSKPLTEEER 181
Query: 177 EKIAKHARALVRFARRIVNIFATQEPRGAKIEVPMVTSEYL----------WGDELIAGN 226
+++ + LV A R +N+ +E E ++ Y ++ GN
Sbjct: 182 DELLTLSDNLVETAERTLNV--GKEMVENLKEKDLMNLGYFESYHMGLVNNGAQDIYEGN 239
Query: 227 DR-------IQYE--------DIEEVPVPYSFAKHSYY-----KGKPIF-VGALARLLLK 265
R I+YE I E PYS+ K +GK + V L+RL +
Sbjct: 240 IRVVNSEGKIEYEFDPAEYANYITEGVRPYSYLKFLALLKRRGEGKGAYRVNTLSRLNVC 299
Query: 266 GSKLEGEAKRLLDSY-KEKIKSGH-VIYNNLAQAIELVYALEVASKIAE-ELISEGIEKS 322
A++ + + K K H + + A+ IEL+ + E+ K E + I + ++
Sbjct: 300 DKMPTPIAQKYYEEFVKTYGKPAHQPMLFHYARLIELLSSSELIRKFLEDDKIVDTDVRA 359
Query: 323 TCEPSEKSGEGIGYVEAPRGVLVHHYRIENERIV-WSNTITPTAFNQGMMEKSLLEDARN 381
+P +GEG+G VEAPRG L+HH++ ++ I+ +N + T N M+ + + A
Sbjct: 360 EVDPKNITGEGVGCVEAPRGTLIHHFKTDDRGIINDTNLVVATVQNNPAMDIGVQKVAEK 419
Query: 382 MF--GKESETTLKSKLEEVVRAFDPCISCSVHVV 413
+ ++ + + +E V+RA+DPC+SC+ H +
Sbjct: 420 YIKTPEVAKPHILNHMEMVIRAYDPCLSCATHTI 453
>pir||S72553 hydrogenase large chain - Anabaena variabilis
>gi|2126555|pir||I39735 hydrogenase large chain -
Anabaena variabilis >gi|1032481|emb|CAA55878.1| (X79285)
hydrogenase large subunit [Anabaena variabilis]
Length = 487
Score = 147 bits (367), Expect = 2e-34
Identities = 139/459 (30%), Positives = 214/459 (46%), Gaps = 58/459 (12%)
Query: 6 IELDEFTRVEGIGKAEIVIEN-GTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICA 64
I +D TR+EG K I +++ G V DAR + E R FE GR ++P + ARIC
Sbjct: 5 IVIDPVTRIEGHAKISIYLDDTGQVSDARFHVTEF-RGFEKFCEGRPLWEMPGITARICG 63
Query: 65 ICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPDA 124
IC SH +AS +A + L + + + KLR L G+I++SHAL + L PD+ D+
Sbjct: 64 ICPVSHLLASAKAGDRILSVTIPPTATKLRRLMNLGQILQSHALSFFHLTAPDLLLGMDS 123
Query: 125 -------ISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGF-------GR- 169
+++ EL + G+ ++ G I E + G +IH PGG GR
Sbjct: 124 DPQKRNIFGLIAAQPELARGGIRLRQFGQEIIEVLGGAKIHPAWAVPGGVREPLSVEGRT 183
Query: 170 YPSERELEKIAKHARALVRFARRIVNIFATQEPRG--AKIEVPMVTSEYLWG--DELI-- 223
+ ER E AL RF + + + + G + + +VT + LW D I
Sbjct: 184 HIQERIPEARTIALDALDRFKKLLKDYEKEVQTFGNFPSLFMGLVTPDGLWETYDGYIRF 243
Query: 224 ---AGN------DRIQYED-IEEVPVPYSFAKHSYYK--GKP------------IFVGAL 259
AGN D +Y++ I E P S+ K YY+ G P VG L
Sbjct: 244 VDSAGNIIADKLDPARYQEFIGEAVQPDSYLKSPYYRPLGYPDQHDQCRIDSGMYRVGPL 303
Query: 260 ARLLLKGSKLEGEAKRLLDSYKEKIKSGHV---IYNNLAQAIELVYALEVASKIAE--EL 314
ARL + A R L ++E + SG + + A+ IE++ +E + + ++
Sbjct: 304 ARLNICSHIGTTLADRELREFRE-LTSGTAKSSFFYHYARLIEILACIEHIEMLLDDPDI 362
Query: 315 ISEGIEKSTCEPSEKSGEGIGYVEAPRGVLVHHYRI-ENERIVWSNTITPTAFNQGMMEK 373
+S + E E +G EAPRG L HHY++ EN + N I T N M +
Sbjct: 363 LSNRLRS---EAGVNQLEAVGVSEAPRGTLFHHYQVDENGLLQKVNLIIATGQNNLAMNR 419
Query: 374 SLLEDARNMF-GKESETTLKSKLEEVVRAFDPCISCSVH 411
++ + AR+ G E + +++E +RAFDPC+SCS H
Sbjct: 420 TVAQIARHFIQGTEIPEGMLNRVEAGIRAFDPCLSCSTH 458
>pir||E30315 methyl viologen-reducing hydrogenase alpha chain - Methanobacterium
thermoautotrophicum (strain delta H)
>gi|149733|gb|AAB02351.1| (J04540) mvhA gene product
[Methanobacterium thermoautotrophicum]
Length = 472
Score = 145 bits (361), Expect = 1e-33
Identities = 122/451 (27%), Positives = 201/451 (44%), Gaps = 47/451 (10%)
Query: 6 IELDEFTRVEGIGKAEIVIEN-GTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICA 64
+ ++ TR+EG K + +++ G V+D R+ ++E R FE GR + P + RIC
Sbjct: 4 LTMEPVTRIEGHAKITVRLDDAGNVEDTRLHVMEF-RGFEKFLQGRPIEEAPRIVPRICG 62
Query: 65 ICYASHSIASVRAIENALGIE---VSESVEKLRELALWGEIIESHALHLYLLALPDVYGY 121
IC H +A+ +A++ G E V + K+RE+ WG + SH LH Y LA PD
Sbjct: 63 ICDVQHHLAAAKAVDACFGFEPEDVLPAAYKMREIMNWGSYMHSHGLHFYFLAAPDFIAG 122
Query: 122 PD-----AISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSEREL 176
D ++ ++ + + ++ + GR IH + PGG +
Sbjct: 123 KDRKTRNVFQIIKDAPDIALQAIELRKNALELVRATGGRPIHPTSSTPGGISTELDDETQ 182
Query: 177 EKIAKHARALVRFARRIV---------NIFATQEPRGAKIEVPMVTSEYLWG--DELIAG 225
+ + K A+ V A + NI + + + +W D ++
Sbjct: 183 KDLLKKAQRNVELAEATLELAVPIFEENIDLVNSLGNIETYHTGLVKDGVWDVYDGIVRI 242
Query: 226 NDR----------IQYED-IEEVPVPYSFAKHSYYK--GKP---IFVGALARLLLKGSKL 269
D+ Y D I E PYS+ K Y K G P V L+RL + K+
Sbjct: 243 KDKEGNMFREFKPADYADTIAEHVTPYSWLKFPYIKDLGYPDGVYRVSPLSRLNV-ADKM 301
Query: 270 EGEAKRLLDSYKEKIK----SGHVIYNNLAQAIELVYALEVASKIAEELISEGIEKSTCE 325
A + + +KE + + + + A+ IEL+ E A+ E +S EK
Sbjct: 302 PDAAPKAQEHFKEFRENFGYAQQTLLYHWARLIELLACAECAADALEGDLSG--EKFPDS 359
Query: 326 PSEKSGEGIGYVEAPRGVLVHHYRI-ENERIVWSNTITPTAFNQGMMEKSLLEDARNMF- 383
++G+G+G VEAPRG L HHY EN I +N + T N ME + + A++
Sbjct: 360 LERQAGDGVGIVEAPRGTLTHHYTCDENGLITKANIVVATIQNNPAMEMGIQKVAQDYIK 419
Query: 384 -GKESETTLKSKLEEVVRAFDPCISCSVHVV 413
G E + + + +E V+RA+DPC+SC+ H +
Sbjct: 420 PGVEVDDKIFNLMEMVIRAYDPCLSCATHTI 450
>pir||A69018 probable coenzyme F420 hydrogenase (EC 1.12.99.1) alpha chain -
Methanobacterium thermoautotrophicum (strain Delta H)
>gi|2622238|gb|AAB85623.1| (AE000883) methyl
viologen-reducing hydrogenase, alpha subunit
[Methanobacterium thermoautotrophicum]
Length = 472
Score = 144 bits (359), Expect = 2e-33
Identities = 122/451 (27%), Positives = 201/451 (44%), Gaps = 47/451 (10%)
Query: 6 IELDEFTRVEGIGKAEIVIEN-GTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICA 64
+ ++ TR+EG K + +++ G V+D R+ ++E R FE GR + P + RIC
Sbjct: 4 LTMEPVTRIEGHAKITVHLDDAGNVEDTRLHVMEF-RGFEKFLQGRPIEEAPRIVPRICG 62
Query: 65 ICYASHSIASVRAIENALGIE---VSESVEKLRELALWGEIIESHALHLYLLALPDVYGY 121
IC H +A+ +A++ G E V + K+RE+ WG + SH LH Y LA PD
Sbjct: 63 ICDVQHHLAAAKAVDACFGFEPEDVLPAAYKMREIMNWGSYMHSHGLHFYFLAAPDFIAG 122
Query: 122 PD-----AISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSEREL 176
D ++ ++ + + ++ + GR IH + PGG +
Sbjct: 123 KDRKTRNVFQIIKDAPDIALQAIELRKNALELVRATGGRPIHPTSSTPGGISTELDDETQ 182
Query: 177 EKIAKHARALVRFARRIV---------NIFATQEPRGAKIEVPMVTSEYLWG--DELIAG 225
+ + K A+ V A + NI + + + +W D ++
Sbjct: 183 KDLLKKAQRNVELAEATLELAVPIFEENIDLVNSLGNIETYHTGLVKDGVWDVYDGIVRI 242
Query: 226 NDR----------IQYED-IEEVPVPYSFAKHSYYK--GKP---IFVGALARLLLKGSKL 269
D+ Y D I E PYS+ K Y K G P V L+RL + K+
Sbjct: 243 KDKEGNMFREFKPADYADTIAEHVKPYSWLKFPYIKDLGYPDGVYRVSPLSRLNV-ADKM 301
Query: 270 EGEAKRLLDSYKEKIK----SGHVIYNNLAQAIELVYALEVASKIAEELISEGIEKSTCE 325
A + + +KE + + + + A+ IEL+ E A+ E +S EK
Sbjct: 302 PDAAPKAQEHFKEFRENFGYAQQTLLYHWARLIELLACAECAADALEGDLSG--EKFPDS 359
Query: 326 PSEKSGEGIGYVEAPRGVLVHHYRI-ENERIVWSNTITPTAFNQGMMEKSLLEDARNMF- 383
++G+G+G VEAPRG L HHY EN I +N + T N ME + + A++
Sbjct: 360 LERQAGDGVGIVEAPRGTLTHHYTCDENGLITKANIVVATIQNNPAMEMGIQKVAQDYIK 419
Query: 384 -GKESETTLKSKLEEVVRAFDPCISCSVHVV 413
G E + + + +E V+RA+DPC+SC+ H +
Sbjct: 420 PGVEVDDKIFNLMEMVIRAYDPCLSCATHTI 450
>pir||A70370 hydrogenase large subunit - Aquifex aeolicus
>gi|2983358|gb|AAC06945.1| (AE000708) hydrogenase large
subunit [Aquifex aeolicus]
Length = 416
Score = 139 bits (347), Expect = 5e-32
Identities = 128/429 (29%), Positives = 196/429 (44%), Gaps = 38/429 (8%)
Query: 4 MIIELDEFTRVEGIGKAEIVIENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARIC 63
M IE TRVEG +V E G +KDA++ R E + R +MD + RIC
Sbjct: 1 MKIEKLVLTRVEGEASLNLVWERGVIKDAKISFYS-TRGIEKVLRERPFMDALVINPRIC 59
Query: 64 AICYASHSIASVRAIENALGI-EVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYP 122
IC +H IA+VRAIENA+GI E+ E + R + E++++H YL +PD +
Sbjct: 60 GICGHAHLIATVRAIENAIGIKEIPEKAKITRLVTQITEMVQNHVKWFYLFVMPDFLKFK 119
Query: 123 DAISMVSKY-GELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELEKIAK 181
+++S + GE K + + I G+ H PGG SERE+ K
Sbjct: 120 ESLSQFEPFKGERWKRAVQFSSQIVKIIALFGGQWPHSSYAVPGGITSNFSEREVLKALN 179
Query: 182 HARALVRFARRIVN----IFATQEPRGAKIEVPMVTSEYLWGDELI--------AGNDRI 229
+ F + V+ +F + + + +L G L G ++
Sbjct: 180 LVKEGKTFFEKNVSEDLELFLNLCEEFNLLSIGKAYNRFLSGGGLAYCFNPSYKKGKGKV 239
Query: 230 QYEDIEEV----PVPYSFAKHSYYKGKPIFVGALARLLLKGSKLEGEA-KRLLDSYKEKI 284
++ V YS A YKG P G LAR L+ + L + K DSY ++
Sbjct: 240 CKFNVRYVQELEAADYSKANPVRYKGLPYETGPLARELISKNPLVLKLYKNYGDSYAVRV 299
Query: 285 KSGHVIYNNLAQAIELVYALEVASKIAEELISEGIEKSTC---EPSEKSGEGIGYVEAPR 341
A+ E+ LE+ K+ +EL++ +E+ +C + S++SGEG G VEA R
Sbjct: 300 ---------AARLAEIKDLLEILEKLLKELMNH-LEEPSCLWEDRSDESGEGFGVVEAAR 349
Query: 342 GVLVHHYRIENERIVWSNTITPTAFNQGMMEKSLLEDA-RNMFGKESETTLKSKLEEVVR 400
G L+H IE +I ITP+ +N G K L A + + G +SE K + V+R
Sbjct: 350 GTLIHRVVIEKGKIKDYKVITPSQWNLGPRCKKYLGVAEKAIVGLDSEL----KAQMVLR 405
Query: 401 AFDPCISCS 409
+FD C C+
Sbjct: 406 SFDLCSVCT 414
>pir||S74233 bidirectional hydrogenase chain H - Synechococcus sp. (PCC 6301)
>gi|1708772|emb|CAA66383.1| (X97797) hoxH [Synechococcus
PCC6301]
Length = 476
Score = 136 bits (339), Expect = 5e-31
Identities = 124/456 (27%), Positives = 202/456 (44%), Gaps = 60/456 (13%)
Query: 6 IELDEFTRVEGIGKAEIVIEN-GTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICA 64
I +D TR+EG K + +++ G + AR ++E R FE GR + ++ + ARIC
Sbjct: 5 IVIDPVTRIEGHAKISVFLDDQGNAEAARFHVVEY-RGFEKFCEGRPFTEMAGITARICG 63
Query: 65 ICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPD-VYGYP- 122
IC SH +A+ + + L +++ + E LR L +II+SHAL + L+ PD + G+
Sbjct: 64 ICPVSHLLAAAKTGDKILAVQIPPAAENLRRLINLAQIIQSHALSFFHLSSPDFLLGWDS 123
Query: 123 -----DAISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELE 177
+ +++ E + G+ ++ G + E + GR+IH PGG S+ +
Sbjct: 124 DPARRNLFGLIAADPESARAGIRLRQFGQQLIEWLGGRKIHAAWAVPGGVRSLLSQEGMV 183
Query: 178 KIAKHARALVRFARRIVNIFATQEPRGAKIEVPMVTSEYLWGDELIAGN----------- 226
I A + R ++ F R + E + L+A N
Sbjct: 184 WIRDRLPAELATVRATLDRFKRLLDREFREETAVFGDFPSLFMGLVADNGDWEHYGGHLR 243
Query: 227 ----------DRIQYED----IEEVPVPYSFAKHSYYK--GKP---IFVGALARLLL--- 264
DR++ +D + E P+S+ K YYK G P VG LARL +
Sbjct: 244 FVDSQGRIIADRLREDDYASFLGEAVEPWSYLKFPYYKPWGYPEGMYRVGPLARLNVCDR 303
Query: 265 -----KGSKLEGEAKRLLDSYKEKIKSGHVIYNNLAQAIELVYALEVASKIAEELISEGI 319
GS+ G A+ + G V + L LV L KIAE + +
Sbjct: 304 IGTGRGGSRATGTARS---------RGGTVTSSFLYHYARLVEILASLEKIAEMVEDPNL 354
Query: 320 EKS--TCEPSEKSGEGIGYVEAPRGVLVHHYRIENE-RIVWSNTITPTAFNQGMMEKSLL 376
+ + E IG EAPRG L HHYR+++ +I N I T N M +++
Sbjct: 355 QTGFLRSQAGVNCLEAIGVSEAPRGTLFHHYRVDSHGKIERVNLIIATGQNNLAMNRTVT 414
Query: 377 EDARNMFGK-ESETTLKSKLEEVVRAFDPCISCSVH 411
+ A++ E + + +++E +R FDPC+SCS H
Sbjct: 415 QIAQHYIRHGEVQESFLNRVEAGIRCFDPCLSCSTH 450
>pir||S75530 hydrogenase large chain - Synechocystis sp. (strain PCC 6803)
>gi|1653175|dbj|BAA18091.1| (D90911) hydrogenase large
subunit [Synechocystis sp.] >gi|1771720|emb|CAA66212.1|
(X97610) hydrogenase subunit [Synechocystis sp.]
Length = 474
Score = 130 bits (324), Expect = 3e-29
Identities = 121/451 (26%), Positives = 206/451 (44%), Gaps = 51/451 (11%)
Query: 6 IELDEFTRVEGIGKAEIVI-ENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICA 64
I +D TR+EG K I + + G V D R ++E R FE GR ++ + ARIC
Sbjct: 5 IVIDPVTRIEGHAKISIFLNDQGNVDDVRFHVVEY-RGFEKFCEGRPMWEMAGITARICG 63
Query: 65 ICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPD-VYGYP- 122
IC SH + + + + L +++ + EKLR L G+I +SHAL + L+ PD + G+
Sbjct: 64 ICPVSHLLCAAKTGDKLLAVQIPPAGEKLRRLMNLGQITQSHALSFFHLSSPDFLLGWDS 123
Query: 123 -----DAISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELE 177
+ +++ +L + G+ ++ G + E + ++IH PGG SE +
Sbjct: 124 DPATRNVFGLIAADPDLARAGIRLRQFGQTVIELLGAKKIHSAWSVPGGVRSPLSEEGRQ 183
Query: 178 KIA---KHARALVRFA----RRIVNIFATQEPRGAK---IEVPMVTSEYLW--------- 218
I A+ V A + +++ F T+ K + + +V W
Sbjct: 184 WIVDRLPEAKETVYLALNLFKNMLDRFQTEVAEFGKFPSLFMGLVGKNNEWEHYGGSLRF 243
Query: 219 ---GDELIAGN-DRIQYED-IEEVPVPYSFAKHSYYK--GKP---IFVGALARLLLKGSK 268
++A N Y D I E +S+ K YYK G P VG LARL +
Sbjct: 244 TDSEGNIVADNLSEDNYADFIGESVEKWSYLKFPYYKSLGYPDGIYRVGPLARLNVCHHI 303
Query: 269 LEGEAKRLLDSYKEKIKSGHV----IYNNLAQAIELVYALEVASKIAEELISEGIEKSTC 324
EA + L+ Y++ ++G V + + A+ +E++ LE + + I C
Sbjct: 304 GTPEADQELEEYRQ--RAGGVATSSFFYHYARLVEILACLEAIELL---MADPDILSKNC 358
Query: 325 EPSEKSG--EGIGYVEAPRGVLVHHYRIENERIVWS-NTITPTAFNQGMMEKSLLEDARN 381
+ E +G EAPRG L HHY+I+ + ++ N I T N M K++ + A++
Sbjct: 359 RAKAEINCTEAVGVSEAPRGTLFHHYKIDEDGLIKKVNLIIATGNNNLAMNKTVAQIAKH 418
Query: 382 MF-GKESETTLKSKLEEVVRAFDPCISCSVH 411
+ + +++E +R +DPC+SCS H
Sbjct: 419 YIRNHDVQEGFLNRVEAGIRCYDPCLSCSTH 449
>gb|AAB53705.1| (U88400) bidirectional hydrogenase large subunit [Prochlorothrix
hollandica]
Length = 482
Score = 127 bits (315), Expect = 3e-28
Identities = 119/452 (26%), Positives = 201/452 (44%), Gaps = 52/452 (11%)
Query: 6 IELDEFTRVEGIGKAEIVIEN-GTVKDA-RVKILEGPRFFEVLTLGRHYMDVPDLEARIC 63
I +D TR+EG+ K I +++ G V DA R ++E R FE GR Y ++ + ARIC
Sbjct: 5 IVIDPVTRIEGLAKISIFLDDAGKVDDAARFHVVEY-RGFEKFCEGRAYTEMAGITARIC 63
Query: 64 AICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPD-VYGYP 122
IC SH +A+ + + L ++V + EKLR L ++ +SHAL + L+ PD + G+
Sbjct: 64 GICPVSHLLAAAKTGDKILAVKVPIAAEKLRRLMNLAQLTQSHALSFFHLSSPDFLLGWD 123
Query: 123 ------DAISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSEREL 176
+ ++ +L + G+ ++ G I E + R+IH PGG +E
Sbjct: 124 SDPARRNVFGLIDANPDLARAGIRLRQFGQTIIELLGARKIHAAWTVPGGVRSPLTEESR 183
Query: 177 EKIAKHARALVRFARRIVNIFATQEPRGAKIEV-----------PMVTSEYLW---GDEL 222
+ I + +++F + K EV +V ++ W G L
Sbjct: 184 QWILDRLPESFATLQTALDLFKGLLDKALKDEVGIFGEFPSLFMSLVAADGAWEHYGGHL 243
Query: 223 --IAGNDRIQYEDIEEVPV---------PYSFAKHSYYK--GKP---IFVGALARL---- 262
+ G I +++ E +S+ K YY+ G P VG LARL
Sbjct: 244 RFVDGQGTIVADNLSEDNYLDFLGEGVEKWSYLKFPYYRPLGYPAGIYRVGPLARLNTCS 303
Query: 263 LLKGSKLEGEAKRLLDSYKEKIKSGHVIYNNLAQAIELVYALEVASKIAEELISEGIEKS 322
+ + + E + + + S + + A+ IE++ LE KI + + +
Sbjct: 304 HIGTPRADAELGEMSPRFHAPVTSSFLYH--YARLIEILACLE---KIHHLMADPDLLSN 358
Query: 323 TCEPS--EKSGEGIGYVEAPRGVLVHHYRI-ENERIVWSNTITPTAFNQGMMEKSLLEDA 379
C + E +G EAPRG L HHY++ EN I N I T N M K++ + A
Sbjct: 359 RCRATGGVNELEAVGVSEAPRGTLFHHYKVDENGLIEKVNLIIATGQNNLAMNKTVTQIA 418
Query: 380 RNMFGKESETTLKSKLEEVVRAFDPCISCSVH 411
++ + +++E + +DPC+SCS H
Sbjct: 419 KHYPRSRYSRSFLNRVEAGIPCYDPCLSCSTH 450
>gb|AAB85780.1| (AE000895) coenzyme F420-reducing hydrogenase, alpha subunit
[Methanobacterium thermoautotrophicum]
Length = 408
Score = 120 bits (298), Expect = 3e-26
Identities = 113/415 (27%), Positives = 187/415 (44%), Gaps = 60/415 (14%)
Query: 18 GKAEIVIE---NGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICAICYASHSIAS 74
G AE+V+E G V R + R E + G+ P + RIC +C H++AS
Sbjct: 18 GHAELVMEVDDEGIVTKGRYFSITPVRGLEKIVTGKAPETAPVIVQRICGVCPIPHTLAS 77
Query: 75 VRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYG---YPDAISMVSKY 131
V AI+++L IEV ++ LREL L + SHA+H +L+A PD DAI+ VS+
Sbjct: 78 VEAIDDSLDIEVPKAGRLLRELTLAAHHVNSHAIHHFLIA-PDFVPENLMADAINSVSE- 135
Query: 132 GELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELEKIAKHARALVRFAR 191
I+ + + +AG IH +V+ GG +E +++ + L
Sbjct: 136 ---------IRKNAQYVVDMVAGEGIHPSDVRIGGMADNITELARKRLYARLKQLKPKVD 186
Query: 192 RIVNIFA-----TQEPRGAKI-EVPMVTSEYLWGDELIAGNDRIQ-------YEDIEEVP 238
V + P+G + P + S ++GD DR Y+D E
Sbjct: 187 EHVELMIGLIEDKGLPKGLGVHNQPTLASHQIYGDRTKFDLDRFTEVMPESWYDDPEIAK 246
Query: 239 VPYSFAKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIKSGHVIYNNLAQAI 298
S Y G+ + VG AR++ + +G +KE+ V+ ++A+A+
Sbjct: 247 RACSTI--PLYDGRNVEVGPRARMV----EFQG--------FKER----GVVAQHVARAL 288
Query: 299 ELVYALEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGVLVHHYRIENERIVWS 358
E+ AL A +I +EL + ++ + G+G +E PRG+ VH ++EN +I +
Sbjct: 289 EMKTALARAIEILDELDTSAPVRADFDERGTGKLGVGAIEGPRGLDVHMAQVENGKIQFY 348
Query: 359 NTITPTAFNQGMMEKSLLEDARNMFGKESETTLKSKLEEVVRAFDPCISCSVHVV 413
+ + PT +N M G +E V+RA+DPC+SC+ HV+
Sbjct: 349 SALVPTTWNIPTM------------GPATEGFHHEYGPHVIRAYDPCLSCATHVM 391
>gb|AAD38065.1|AF153438_1 (AF153438) HoxH [Rhodobacter capsulatus]
Length = 503
Score = 120 bits (298), Expect = 3e-26
Identities = 119/448 (26%), Positives = 200/448 (44%), Gaps = 63/448 (14%)
Query: 16 GIGKAEIVIE-NGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICAICYASHSIAS 74
G G+ I ++ G V+ ++ +L+ E L GR ++P+ R+C +C SH +A+
Sbjct: 35 GQGRVTICLDARGQVRQVQLHLLDLCGV-ETLLEGRAIAEIPEAVQRLCGLCPVSHHLAA 93
Query: 75 VRAIENALGIE-VSESVEKLRELALWGEIIESHALHLYLLALPD-VYGYPD------AIS 126
V+A++ G + ++ + EKLR L +G+I++SHA+ LY LA PD + G+ +
Sbjct: 94 VKAMDRIAGYDRLTPTAEKLRRLMQYGQIVQSHAVQLYHLAEPDLLLGFDSEPARRGLVD 153
Query: 127 MVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSER--------ELEK 178
+ + +L EG+ ++ G + I G + G PGG R P R ++++
Sbjct: 154 VARAFPQLATEGVLMRRFGQEVVRAITGGRLRGAGAVPGGM-RRPLPRAARDQLRADVDQ 212
Query: 179 IAKHARALVRFARRIVN---IFATQEPRGA----KIEVPMVTSEYLWGDELIAGNDRIQY 231
+ A +R R+ F PR A + P ++ D + D
Sbjct: 213 VIDWAAGALRVLERLHGTNPAFYDHPPRPAATSWRWSAPSGAVDFY--DGRLRARDARGA 270
Query: 232 EDIEEVPV---------------PYSFA---KHSYYKGKPIFVGALARL----LLKGSKL 269
+ VP P+ A + +Y+ VG LARL LL K
Sbjct: 271 TLFDAVPAAAYRGVLAEPSGPGGPHLRALGPETGWYR-----VGPLARLQVCDLLTSEKA 325
Query: 270 EGEAKRLLDSYKEKIKSGHVIYNNLAQAIELVYALEVASKIAE--ELISEGIEKSTCEPS 327
E E +RLL K G V ++ A+ +EL++A E+ + + EL+ + P
Sbjct: 326 ETERQRLLAPGDGKPLQGSVFHHG-ARLVELLHAAELIRDLLDDPELLGTDLMADRGAPQ 384
Query: 328 EKSGEGIGYVEAPRGVLVHHYRI-ENERIVWSNTITPTAFNQGMMEKSLL-EDARNMFGK 385
E +G +EAPRG L+ Y + E RI + T N M+ +L A+ G+
Sbjct: 385 R---EAVGVIEAPRGTLLQRYEVDEAGRIRRCTLLLSTRNNSRAMDAALQGVAAQYCDGR 441
Query: 386 ESETTLKSKLEEVVRAFDPCISCSVHVV 413
L + +E +RAFDPC+SC+ H +
Sbjct: 442 RVTEDLLAHIEVAIRAFDPCLSCAAHAL 469
>sp|P19496|FRHA_METTH COENZYME F420 HYDROGENASE ALPHA SUBUNIT
(8-HYDROXY-5-DEAZAFLAVIN-REDUCING HYDROGENASE ALPHA
SUBUNIT) (FRH) >gi|80912|pir||A35620 coenzyme F420
hydrogenase (EC 1.12.99.1) alpha chain -
Methanobacterium thermoautotrophicum
>gi|551889|gb|AAA72187.1| (J02914) F420 hydrogenase
alpha-subunit (frhA) [Methanobacterium
thermoautotrophicum]
Length = 405
Score = 120 bits (298), Expect = 3e-26
Identities = 113/415 (27%), Positives = 187/415 (44%), Gaps = 60/415 (14%)
Query: 18 GKAEIVIE---NGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICAICYASHSIAS 74
G AE+V+E G V R + R E + G+ P + RIC +C H++AS
Sbjct: 15 GHAELVMEVDDEGIVTKGRYFSITPVRGLEKIVTGKAPETAPVIVQRICGVCPIPHTLAS 74
Query: 75 VRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYG---YPDAISMVSKY 131
V AI+++L IEV ++ LREL L + SHA+H +L+A PD DAI+ VS+
Sbjct: 75 VEAIDDSLDIEVPKAGRLLRELTLAAHHVNSHAIHHFLIA-PDFVPENLMADAINSVSE- 132
Query: 132 GELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELEKIAKHARALVRFAR 191
I+ + + +AG IH +V+ GG +E +++ + L
Sbjct: 133 ---------IRKNAQYVVDMVAGEGIHPSDVRIGGMADNITELARKRLYARLKQLKPKVD 183
Query: 192 RIVNIFA-----TQEPRGAKI-EVPMVTSEYLWGDELIAGNDRIQ-------YEDIEEVP 238
V + P+G + P + S ++GD DR Y+D E
Sbjct: 184 EHVELMIGLIEDKGLPKGLGVHNQPTLASHQIYGDRTKFDLDRFTEVMPESWYDDPEIAK 243
Query: 239 VPYSFAKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIKSGHVIYNNLAQAI 298
S Y G+ + VG AR++ + +G +KE+ V+ ++A+A+
Sbjct: 244 RACSTI--PLYDGRNVEVGPRARMV----EFQG--------FKER----GVVAQHVARAL 285
Query: 299 ELVYALEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGVLVHHYRIENERIVWS 358
E+ AL A +I +EL + ++ + G+G +E PRG+ VH ++EN +I +
Sbjct: 286 EMKTALARAIEILDELDTSAPVRADFDERGTGKLGVGAIEGPRGLDVHMAQVENGKIQFY 345
Query: 359 NTITPTAFNQGMMEKSLLEDARNMFGKESETTLKSKLEEVVRAFDPCISCSVHVV 413
+ + PT +N M G +E V+RA+DPC+SC+ HV+
Sbjct: 346 SALVPTTWNIPTM------------GPATEGFHHEYGPHVIRAYDPCLSCATHVM 388
>emb|CAA43500.1| (X61202) F420-reducing hydrogenase subunit [Methanococcus voltae]
Length = 411
Score = 119 bits (295), Expect = 7e-26
Identities = 115/411 (27%), Positives = 187/411 (44%), Gaps = 44/411 (10%)
Query: 18 GKAEIVI---ENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICAICYASHSIAS 74
G A++V+ ++G V A R FE + G+ P RIC IC A+H IAS
Sbjct: 15 GHAKLVLNVDDDGIVTKAFYPNTTPVRGFETMLQGKPAAFGPIAVMRICGICQATHGIAS 74
Query: 75 VRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPDAISMVSKYGEL 134
AIENA+G E+ + LREL G + SH LH +LL + D+ +A ++ +L
Sbjct: 75 CEAIENAIGAEIPQDGAILRELLGLGNRMHSHPLH-HLLIVDDLLKPEEADTLRIDGIKL 133
Query: 135 IKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELEKIAKHARALVRFARRIV 194
I++ ++ G +I + + G IH N+ GG +ER K+ ++ AR++
Sbjct: 134 IQK---MRKEGQLIVDIVGGEGIHPPNLVIGGMRNNITERAKSKLYYASKEYQEDARQMY 190
Query: 195 NIFATQEPRGA-KIEVPMVTSE---YLWGDELIAGNDRIQYEDIEEVPVPYSFAKHSYYK 250
++ R +I +P + Y+ D D ++ +DI E+ +YY
Sbjct: 191 DLIKMLTERYLDEIGIPDLGRHDLPYIATDTTFGNRDNLKLDDIAEL------TAQNYYS 244
Query: 251 GKPIFVGALARL--LLKGSKLEGEAKRLLDSYKEKIKSGHVIYNNLAQAIELVYALEVAS 308
P L G+ EG + + + G V+ NLA+A+E + A+E +
Sbjct: 245 NYPEIAQTATNQIPLYLGTPAEGGPRARMVKFGSFRTDGGVMDVNLARALENLGAVERSL 304
Query: 309 KIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGVLVH------HYRIENERIVWSNTIT 362
++ EEL EG K T P K G G+G EAPR H RI+N RI+ ++T
Sbjct: 305 ELLEELNIEGATKIT--PEYKDGFGVGVHEAPRATNTHMAEMGADGRIKNYRIIAAST-- 360
Query: 363 PTAFNQGMMEKSLLEDARNMFGKESETTLKSKLEEVVRAFDPCISCSVHVV 413
+N ++EK++ E E ++RA+D SC+ H++
Sbjct: 361 ---WNMPVVEKAI------------EGYHHKYAEVIMRAYDIXASCATHII 396
>sp|Q60338|FRHA_METJA COENZYME F420 HYDROGENASE ALPHA SUBUNIT
(8-HYDROXY-5-DEAZAFLAVIN-REDUCING HYDROGENASE ALPHA
SUBUNIT) (FRH) >gi|2127832|pir||A64299 coenzyme F420
hydrogenase (EC 1.12.99.1) alpha chain [similarity] -
Methanococcus jannaschii
Length = 415
Score = 113 bits (280), Expect = 4e-24
Identities = 117/423 (27%), Positives = 183/423 (42%), Gaps = 48/423 (11%)
Query: 6 IELDEFTRVEGIGKAEIVI-ENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICA 64
IE+ TR EG K + + E G V A R FE + G+ P RIC
Sbjct: 10 IEIAPTTRHEGHAKLILEVDEEGIVNKAYYLNTTPVRGFETMLKGKPAEFAPIAVMRICG 69
Query: 65 ICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPDA 124
IC +H IAS AIENA+ EV + LREL G + SH LH +LL + D + PD
Sbjct: 70 ICQTTHGIASCEAIENAIDCEVPDDGLLLRELVGIGNRLHSHPLH-HLLTIDD-FLKPDE 127
Query: 125 ISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSER-------ELE 177
+ ELIK ++ +G ++ + + G IH N+ GG +ER L
Sbjct: 128 TDLKI---ELIKLIQRMRKVGQLVVDIVGGEGIHPPNIVIGGMRTNITERAKSRLYYALR 184
Query: 178 KIAKHARALVRFARRIVNIFATQ--EPRGAKIEVPMVTSEYLWGDELIAGNDRIQYEDIE 235
+ K A L ++ + + P E P + + +GD I ++D+
Sbjct: 185 QYEKDAYELYEKYTELIERYLEEIGIPDLGAHEYPYIATHTTYGDRY-----AINWDDVT 239
Query: 236 EVPVPYSF----AKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIKSGHVIY 291
E+P + AK + P++ G A EG + + + + G +
Sbjct: 240 EIPAQRYYDDEEAKQTTTIQIPLYAGVPA---------EGGPRARMVKFGNFREGGSAMD 290
Query: 292 NNLAQAIELVYALEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGVLVHHYRI- 350
N+A+A E + A+ A +I +EL G K+ E K G GIG EAPR H +
Sbjct: 291 INIARAQENLGAVYRALEILDELDLNG--KTRAEVEYKDGFGIGVHEAPRATNTHMAEVG 348
Query: 351 ENERIVWSNTITPTAFNQGMMEKSLLEDARNMFGKESETTLKSKLEEVVRAFDPCISCSV 410
++ +I I + +N ++EK++ E + E ++RA+D C SC+
Sbjct: 349 KDGKIKSYRIIAASTWNFPIVEKAI------------EGYPQQYAEVIMRAYDICASCAT 396
Query: 411 HVV 413
HV+
Sbjct: 397 HVI 399
>sp|Q00390|FRHA_METVO COENZYME F420 HYDROGENASE ALPHA SUBUNIT
(8-HYDROXY-5-DEAZAFLAVIN-REDUCING HYDROGENASE ALPHA
SUBUNIT) (FRH) >gi|99140|pir||S16721 coenzyme F420
hydrogenase (EC 1.12.99.1) alpha chain frcA [similarity]
- Methanococcus voltae >gi|44701|emb|CAA43496.1|
(X61201) F420-reducing hydrogenase subunit
[Methanococcus voltae]
Length = 398
Score = 113 bits (279), Expect = 5e-24
Identities = 115/416 (27%), Positives = 188/416 (44%), Gaps = 42/416 (10%)
Query: 6 IELDEFTRVEGIGKAEIVIEN-GTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICA 64
+E++ TR EG K + +++ G V+ + R FE +G+ P +R C
Sbjct: 5 VEINPTTRHEGHTKLVLKVDDDGIVEKGNYLSVTPVRGFEKFLVGKPAEFAPIAVSRFCG 64
Query: 65 ICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPDA 124
IC +H+ ++V AIE+A GI + LREL G + SH LH +L+A PD D
Sbjct: 65 ICPIAHATSAVEAIEDACGIVPPKDGLLLRELTGLGNKMHSHPLHEFLIA-PDFIPEKDR 123
Query: 125 ISMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELEKIAKHAR 184
+ +++ ++ K G I +TI G IH N+K GG + + + KI +
Sbjct: 124 VEYITRIQQMRK-------TGQYIVDTIGGEAIHAPNIKVGGMLKSITPSAVSKIYYKCK 176
Query: 185 ALVRFARR----IVNIFATQE-PRGAKIEVPMVTSE--YLWGDELIAGNDRIQYEDIEEV 237
+ A+ ++ IF + G +I + + YL D + I+ + + E
Sbjct: 177 EFEKLAKEQVEYLIPIFENRTLVDGTEIPEKLGYHDFGYLATDSTYGNRENIEQKKVHEY 236
Query: 238 PVPYS-FAKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIKSGHVIYNNLAQ 296
PY + K + +F RL+ G + R + K K I+ +A+
Sbjct: 237 -TPYDVYEKEVAVQACQLFQEYNGRLMEVGPR-----ARFAKFHDFKEKGAMAIH--IAR 288
Query: 297 AIELVYALEVASKIAEELISEGIEKSTCEPSEKSGE--GIGYVEAPRGVLVHHYRI-ENE 353
A E V ++ A +I EEL +G+ ++ +P GE G+G EA RG H I E
Sbjct: 289 AYENVIHVKRAMEIIEELNVDGLTRAK-DPILGDGEKLGLGVHEAARGHNTHQASIDEKG 347
Query: 354 RIVWSNTITPTAFNQGMMEKSLLEDARNMFGKESETTLKSKLEEVVRAFDPCISCS 409
RI + N I T +N ++ K++ +A F E VVRA+DPC+SC+
Sbjct: 348 RITYYNAIVATTWNIPLIGKAV--EAHYKFA-----------EHVVRAYDPCVSCA 390
>pir||HQDVLB cytochrome-c3 hydrogenase (EC 1.12.2.1) (NiFeSe) large chain -
Desulfovibrio baculatus
Length = 514
Score = 107 bits (265), Expect = 2e-22
Identities = 114/495 (23%), Positives = 195/495 (39%), Gaps = 95/495 (19%)
Query: 6 IELDEFTRVEGIGKAEIVIENGTVKDARVKILEGPRF--FEVLTLGRHYMDVPDLEARIC 63
I +D TRVEG K E+ +++G V DA+ G F FE + GR D + RIC
Sbjct: 15 ISIDPLTRVEGHLKIEVEVKDGKVVDAKCS---GGMFRGFEQILRGRDPRDSSQIVQRIC 71
Query: 64 AICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPD 123
+C +H ASV A ++A G++V+ + R L ++SH LH Y LA D PD
Sbjct: 72 GVCPTAHCTASVMAQDDAFGVKVTTNGRITRNLIFGANYLQSHILHFYHLAALDYVKGPD 131
Query: 124 AISMVSKYG------ELIKEG-----------------LTIKALGNMIRETIAGREIHGI 160
V +Y + IK+G L I+ + + + GR H
Sbjct: 132 VSPFVPRYANADLLTDRIKDGAKADATNTYGLNQYLKALEIRRICHEMVAMFGGRMPHVQ 191
Query: 161 NVKPGGFGRYPSERELEKIAKHARALVRFARRIVNIFATQEPRGAKIEVPMVTSEYLWGD 220
+ GG P+ +K+A++A + ++ + + + + W +
Sbjct: 192 GMVVGGATEIPT---ADKVAEYAARFKEVQKFVIEEYLPLIYTLGSVYTDLFETGIGWKN 248
Query: 221 EL---------------------IAGNDRIQYEDIEEVPVPYSFAKHS------------ 247
+ I G D + + V +SF HS
Sbjct: 249 VIAFGVFPEDDDYKTFLLKPGVYIDGKDEEFDSKLVKEYVGHSFFDHSAPGGLHYSVGET 308
Query: 248 ----------------YYKGKPIFVGALARLLLKGSKLEGEAKRLL--------DSYKEK 283
YK KP VG LAR+ ++ +L ++LL +++
Sbjct: 309 NPNPDKPGAYSFVKAPRYKDKPCEVGPLARMWVQNPELSPVGQKLLKELYGIEAKKFRDL 368
Query: 284 IKSGHVIYNNLAQAIELVYALEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGV 343
I E + VA + + + G E + EG G+ EAPRG
Sbjct: 369 GDKAFSIMGRHVARAEETWLTAVAVEKWLKQVQPGAETYVKSEIPDAAEGTGFTEAPRGA 428
Query: 344 LVHHYRIENERIVWSNTITPTAFN----QGMMEKSLLEDARNMFG-KESETTLKSKLEEV 398
L+H+ +I++++I ++ T +N M ++ +E+A + G + + +
Sbjct: 429 LLHYLKIKDKKIENYQIVSATLWNANPRDDMGQRGPIEEA--LIGVPVPDIKNPVNVGRL 486
Query: 399 VRAFDPCISCSVHVV 413
VR++DPC+ C+VHV+
Sbjct: 487 VRSYDPCLGCAVHVL 501
>sp|P13065|PHSL_DESBA PERIPLASMIC [NIFESE] HYDROGENASE LARGE SUBUNIT (NIFESE
HYDROGENLYASE LARGE CHAIN)
Length = 514
Score = 107 bits (265), Expect = 2e-22
Identities = 114/495 (23%), Positives = 195/495 (39%), Gaps = 95/495 (19%)
Query: 6 IELDEFTRVEGIGKAEIVIENGTVKDARVKILEGPRF--FEVLTLGRHYMDVPDLEARIC 63
I +D TRVEG K E+ +++G V DA+ G F FE + GR D + RIC
Sbjct: 15 ISIDPLTRVEGHLKIEVEVKDGKVVDAKCS---GGMFRGFEQILRGRDPRDSSQIVQRIC 71
Query: 64 AICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPD 123
+C +H ASV A ++A G++V+ + R L ++SH LH Y LA D PD
Sbjct: 72 GVCPTAHCTASVMAQDDAFGVKVTTNGRITRNLIFGANYLQSHILHFYHLAALDYVKGPD 131
Query: 124 AISMVSKYG------ELIKEG-----------------LTIKALGNMIRETIAGREIHGI 160
V +Y + IK+G L I+ + + + GR H
Sbjct: 132 VSPFVPRYANADLLTDRIKDGAKADATNTYGLNQYLKALEIRRICHEMVAMFGGRMPHVQ 191
Query: 161 NVKPGGFGRYPSERELEKIAKHARALVRFARRIVNIFATQEPRGAKIEVPMVTSEYLWGD 220
+ GG P+ +K+A++A + ++ + + + + W +
Sbjct: 192 GMVVGGATEIPT---ADKVAEYAARFKEVQKFVIEEYLPLIYTLGSVYTDLFETGIGWKN 248
Query: 221 EL---------------------IAGNDRIQYEDIEEVPVPYSFAKHS------------ 247
+ I G D + + V +SF HS
Sbjct: 249 VIAFGVFPEDDDYKTFLLKPGVYIDGKDEEFDSKLVKEYVGHSFFDHSAPGGLHYSVGET 308
Query: 248 ----------------YYKGKPIFVGALARLLLKGSKLEGEAKRLL--------DSYKEK 283
YK KP VG LAR+ ++ +L ++LL +++
Sbjct: 309 NPNPDKPGAYSFVKAPRYKDKPCEVGPLARMWVQNPELSPVGQKLLKELYGIEAKKFRDL 368
Query: 284 IKSGHVIYNNLAQAIELVYALEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGV 343
I E + VA + + + G E + EG G+ EAPRG
Sbjct: 369 GDKAFSIMGRHVLVAEETWLTAVAVEKWLKQVQPGAETYVKSEIPDAAEGTGFTEAPRGA 428
Query: 344 LVHHYRIENERIVWSNTITPTAFN----QGMMEKSLLEDARNMFG-KESETTLKSKLEEV 398
L+H+ +I++++I ++ T +N M ++ +E+A + G + + +
Sbjct: 429 LLHYLKIKDKKIENYQIVSATLWNANPRDDMGQRGPIEEA--LIGVPVPDIKNPVNVGRL 486
Query: 399 VRAFDPCISCSVHVV 413
VR++DPC+ C+VHV+
Sbjct: 487 VRSYDPCLGCAVHVL 501
>pdb|1CC1|L Chain L, Crystal Structure Of A Reduced, Active Form Of The
Ni-Fe-Se Hydrogenase From Desulfomicrobium Baculatum
Length = 498
Score = 101 bits (248), Expect = 2e-20
Identities = 112/493 (22%), Positives = 192/493 (38%), Gaps = 95/493 (19%)
Query: 6 IELDEFTRVEGIGKAEIVIENGTVKDARVKILEGPRF--FEVLTLGRHYMDVPDLEARIC 63
I +D TRVEG K E+ +++G V DA+ G F FE + GR D + RIC
Sbjct: 14 ISIDPLTRVEGHLKIEVEVKDGKVVDAKCS---GGMFRGFEQILRGRDPRDSSQIVQRIC 70
Query: 64 AICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPD 123
+C +H ASV A ++A G++V+ + R L ++SH LH Y LA D PD
Sbjct: 71 GVCPTAHCTASVMAQDDAFGVKVTTNGRITRNLIFGANYLQSHILHFYHLAALDYVKGPD 130
Query: 124 AISMVSKYG------ELIKEG-----------------LTIKALGNMIRETIAGREIHGI 160
V +Y + IK+G L I+ + + + GR H
Sbjct: 131 VSPFVPRYANADLLTDRIKDGAKADATNTYGLNQYLKALEIRRICHEMVAMFGGRMPHVQ 190
Query: 161 NVKPGGFGRYPSERELEKIAKHARALVRFARRIVNIFATQEPRGAKIEVPMVTSEYLWGD 220
+ GG P+ +K+A++A + ++ + + + + W +
Sbjct: 191 GMVVGGATEIPT---ADKVAEYAARFKEVQKFVIEEYLPLIYTLGSVYTDLFETGIGWKN 247
Query: 221 EL---------------------IAGNDRIQYEDIEEVPVPYSFAKHS------------ 247
+ I G D + + V +SF HS
Sbjct: 248 VIAFGVFPEDDDYKTFLLKPGVYIDGKDEEFDSKLVKEYVGHSFFDHSAPGGLHYSVGET 307
Query: 248 ----------------YYKGKPIFVGALARLLLKGSKLEGEAKRLL--------DSYKEK 283
YK KP VG LAR+ ++ +L ++LL +++
Sbjct: 308 NPNPDKPGAYSFVKAPRYKDKPCEVGPLARMWVQNPELSPVGQKLLKELYGIEAKKFRDL 367
Query: 284 IKSGHVIYNNLAQAIELVYALEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGV 343
I E + VA + + + G E + EG G+ EAPRG
Sbjct: 368 GDKAFSIMGRHVLRAEETWLTAVAVEKWLKQVQPGAETYVKSEIPDAAEGTGFTEAPRGA 427
Query: 344 LVHHYRIENERIVWSNTITPTAFN----QGMMEKSLLEDARNMFG-KESETTLKSKLEEV 398
L+H+ +I++++I ++ T +N M ++ +E+A + G + + +
Sbjct: 428 LLHYLKIKDKKIENYQIVSATLWNANPRDDMGQRGPIEEA--LIGVPVPDIKNPVNVGRL 485
Query: 399 VRAFDPCISCSVH 411
VR++DP + C+VH
Sbjct: 486 VRSYDPXLGCAVH 498
>emb|CAA74094.1| (Y13764) F420-reducing hydrogenase isoenzyme I alpha subunit
[Methanosarcina barkeri]
Length = 456
Score = 97.9 bits (240), Expect = 2e-19
Identities = 103/455 (22%), Positives = 177/455 (38%), Gaps = 64/455 (14%)
Query: 5 IIELDEFTRVEGIGKAEIVIENGTVKDARVKILEGP-RFFEVLTLGRHYMDVPDLEARIC 63
++E+ TR EG K + + + + + + P R E L +G+ VP + +R+C
Sbjct: 4 VVEISPTTRHEGHSKLTLKVNDEGIVERGDWLSTTPVRGIEKLAIGKTMDQVPKIASRVC 63
Query: 64 AICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPD 123
IC +H++A + A+E ++G E+ + + LR + + SHALH +L LPD Y PD
Sbjct: 64 GICPIAHTLAGIEAMEASIGCEIPKDAKLLRVILHAANRLHSHALH-NILILPDFY-IPD 121
Query: 124 AISMVSKYGE------LIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELE 177
+ ++ + + + I+ + I G IH N + GG R S R +
Sbjct: 122 TETKINPFSKEQPLRSVAVRIFRIREIAQTIGAVAGGEAIHPSNPRVGGMYRNVSSRAKQ 181
Query: 178 KIAKHARALVRFA----------------RRIVNIFATQEP---------RGAKIEVPMV 212
KIA A+ + A R V + Q P +G PM
Sbjct: 182 KIADLAKEGLVLAHEQMEFMIEVIRNMQDREFVEVAGKQIPLPKTLGYHNQGVMATAPMY 241
Query: 213 TSEYL----------WGD----ELIAGNDRIQYEDIEEVPVPYSFAKHSYYKGKPIFVGA 258
S L W + + + I D+E+ P K P
Sbjct: 242 GSSSLDEKPMWDFTRWRETRPWDWYMSEETI---DLEDSSYPIGGTTKVGTKVNPRMEAC 298
Query: 259 LARLLLKGSKLEGEAKRLLDSYKEKIKSGHVIYNNLAQAIELVYALEVASKIAEELISEG 318
G +E + L ++K + G ++A+ +E E L + G
Sbjct: 299 NTVPTYDGQPVEVGPRARLATFKHFTEKG-TFAQHIARQMEYTDCYYTILNCLENLDTSG 357
Query: 319 IEKSTCEPSEKSGEGIGYVEAPRGVLVHHYRIENERIVWSNTITPTAFNQGMMEKSLLED 378
+ P G EAPRG VH R+++ +++ + PT +N ++L
Sbjct: 358 KVLADTIPLGNGSMGWAANEAPRGTDVHLARVKDGKVLRYEMLVPTTWNFPTCSRALTGA 417
Query: 379 ARNMFGKESETTLKSKLEEVVRAFDPCISCSVHVV 413
+ E V+RA+DPC+SC+ H++
Sbjct: 418 PWQI------------AEMVIRAYDPCVSCATHMI 440
>gb|AAA23375.2| (M18271) large hydrogenase subunit [Desulfomicrobium baculatum]
Length = 514
Score = 94.8 bits (232), Expect = 2e-18
Identities = 113/496 (22%), Positives = 194/496 (38%), Gaps = 97/496 (19%)
Query: 6 IELDEFTRVEGIGKAEIVIENGTVKDARVKILEGPRF--FEVLTLGRHYMDVPDLEARIC 63
I +D TRVEG K E+ +++G V DA+ G F FE + GR D + RIC
Sbjct: 15 ISIDPLTRVEGHLKIEVEVKDGKVVDAKCS---GGMFRGFEQILRGRDPRDSSQIVQRIC 71
Query: 64 AICYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPD 123
+C +H ASV A ++A G++V+ + R L ++SH LH Y LA D PD
Sbjct: 72 GVCPTAHCTASVMAQDDAFGVKVTTNGRITRNLIFGANYLQSHILHFYHLAALDYVKGPD 131
Query: 124 AISMVSKYG------ELIKEG-----------------LTIKALGNMIRETIAGREIHGI 160
V +Y + IK+G L I+ + + + GR H
Sbjct: 132 VSPFVPRYANADLLTDRIKDGAKADATNTYGLNQYLKALEIRRICHEMVAMFGGRMPHVQ 191
Query: 161 NVKPGGFGRYPSERELEKIAKHARALVRFARRIVNIFATQEPRGAKIEVPMVTSEYLWGD 220
+ GG P+ +K+A++A + ++ + + + + W +
Sbjct: 192 GMVVGGATEIPT---ADKVAEYAARFKEVQKFVIEEYLPLIYTLGSVYTDLFETGIGWKN 248
Query: 221 EL---------------------IAGNDRIQYEDIEEVPVPYSFAKHS------------ 247
+ I G D + + V +SF HS
Sbjct: 249 VIAFGVFPEDDDYKTFLLKPGVYIDGKDEEFDSKLVKEYVGHSFFDHSAPGGLHYSVGET 308
Query: 248 ----------------YYKGKPIFVGALARLLLKGSKLEGEAKRLL--------DSYKEK 283
YK KP VG LAR+ ++ +L ++LL +++
Sbjct: 309 NPNPDKPGAYSFVKAPRYKDKPCEVGPLARMWVQNPELSPVGQKLLKELYGIEAKKFRDL 368
Query: 284 IKSGHVIYNNLAQAIELVYALEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGV 343
I E + VA + + + G E + EG G+ EAPRG
Sbjct: 369 GDKAFSIMGRHVLVAEETWLTAVAVEKWLKQVQPGAETYVKSEIPDAAEGTGFTEAPRGA 428
Query: 344 LVHHYRIENERIVWSNTITPTAFN----QGMMEKSLLEDARNMFG-KESETTLKSKLEEV 398
L+H+ +I++++I ++ T +N M ++ +E+A + G + + +
Sbjct: 429 LLHYLKIKDKKIENYQIVSATLWNANPRDDMGQRGPIEEA--LIGVPVPDIKNPVNVGRL 486
Query: 399 VRAFDP-CISCSVHVV 413
VR++DP + C+VHV+
Sbjct: 487 VRSYDPU-LGCAVHVL 501
>pir||F30315 methyl viologen-reducing hydrogenase (EC 1.-.-.-) alpha chain -
Methanobacterium thermoautotrophicum (strain winter)
(fragment)
Length = 242
Score = 77.3 bits (187), Expect = 3e-13
Identities = 40/116 (34%), Positives = 64/116 (54%), Gaps = 5/116 (4%)
Query: 6 IELDEFTRVEGIGKAEIVIEN-GTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICA 64
+ ++ TR+EG K + +++ G V+D R+ ++E R FE GR + P + RIC
Sbjct: 4 LTMEPVTRIEGHAKITVHLDDAGNVEDTRLHVMEF-RGFEKFLQGRPIEEAPRIVPRICG 62
Query: 65 ICYASHSIASVRAIENALGIEVSE---SVEKLRELALWGEIIESHALHLYLLALPD 117
IC H +A+ +A++ G E + + K+RE+ WG + SH LH Y LA PD
Sbjct: 63 ICDVQHHLAAAKAVDACFGFEPDDILPAAYKMREIMNWGSYMHSHGLHFYFLAAPD 118
>sp|P18188|PHNL_DESFR PERIPLASMIC [NIFE] HYDROGENASE LARGE SUBUNIT PRECURSOR (NIFE
HYDROGENLYASE LARGE CHAIN) >gi|2117631|pir||S08199
cytochrome-c3 hydrogenase (EC 1.12.2.1) (NiFe) large
chain, periplasmic - Desulfovibrio fructosovorans
>gi|145097|gb|AAA23372.1| (M35333) periplasmic
hydrogenase-beta subunit (hydB) [Desulfovibrio
fructosovorans]
Length = 564
Score = 73.7 bits (178), Expect = 3e-12
Identities = 56/186 (30%), Positives = 94/186 (50%), Gaps = 15/186 (8%)
Query: 241 YSFAKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIK-SGHVIYNNL----A 295
YS+ K YKGK + VG LAR + +K + + K+++D K+ +++ L A
Sbjct: 368 YSWMKAPRYKGKAMEVGPLARTFIAYAKGQPDFKKVVDMVLGKLSVPATALHSTLGRTAA 427
Query: 296 QAIELVYALEVASKIAEELISEGIEKST-CEPSE--KSGEGIGYVEAPRGVLVHHYRIEN 352
+ IE K +E+ G + +T C E + +G+G +APRG L H RI+
Sbjct: 428 RGIETAIVCANMEKWIKEMADSGAKDNTLCAKWEMPEESKGVGLADAPRGSLSHWIRIKG 487
Query: 353 ERIVWSNTITPTAFNQG----MMEKSLLEDARNMFGKE-SETTLKSKLEEVVRAFDPCIS 407
++I + P+ +N G +KS +E+A + G ++ ++ V AFDPCI+
Sbjct: 488 KKIDNFQLVVPSTWNLGPRGPQGDKSPVEEA--LIGTPIADPKRPVEILRTVHAFDPCIA 545
Query: 408 CSVHVV 413
C VHV+
Sbjct: 546 CGVHVI 551
>pdb|1FRF|L Chain L, Crystal Structure Of The Ni-Fe Hydrogenase From
Desulfovibrio Fructosovorans
Length = 564
Score = 73.7 bits (178), Expect = 3e-12
Identities = 56/186 (30%), Positives = 94/186 (50%), Gaps = 15/186 (8%)
Query: 241 YSFAKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIK-SGHVIYNNL----A 295
YS+ K YKGK + VG LAR + +K + + K+++D K+ +++ L A
Sbjct: 368 YSWMKAPRYKGKAMEVGPLARTFIAYAKGQPDFKKVVDMVLGKLSVPATALHSTLGRTAA 427
Query: 296 QAIELVYALEVASKIAEELISEGIEKST-CEPSE--KSGEGIGYVEAPRGVLVHHYRIEN 352
+ IE K +E+ G + +T C E + +G+G +APRG L H RI+
Sbjct: 428 RGIETAIVCANMEKWIKEMADSGAKDNTLCAKWEMPEESKGVGLADAPRGSLSHWIRIKG 487
Query: 353 ERIVWSNTITPTAFNQG----MMEKSLLEDARNMFGKE-SETTLKSKLEEVVRAFDPCIS 407
++I + P+ +N G +KS +E+A + G ++ ++ V AFDPCI+
Sbjct: 488 KKIDNFQLVVPSTWNLGPRGPQGDKSPVEEA--LIGTPIADPKRPVEILRTVHAFDPCIA 545
Query: 408 CSVHVV 413
C VHV+
Sbjct: 546 CGVHVI 551
>sp|P21852|PHNL_DESVM PERIPLASMIC [NIFE] HYDROGENASE LARGE SUBUNIT PRECURSOR (NIFE
HYDROGENLYASE LARGE CHAIN) >gi|419605|pir||B45865
cytochrome-c3 hydrogenase (EC 1.12.2.1) (NiFe) large
chain - Desulfovibrio vulgaris (strain Miyazaki)
>gi|4558287|pdb|1H2A|L Chain L, Single Crystals Of
Hydrogenase From Desulfovibrio Vulgaris
>gi|145094|gb|AAA23370.1| (M58339) [NiFe] hydrogenase
large subunit [Desulfovibrio vulgaris]
Length = 567
Score = 71.8 bits (173), Expect = 1e-11
Identities = 56/186 (30%), Positives = 90/186 (48%), Gaps = 14/186 (7%)
Query: 241 YSFAKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIKSG-HVIYNNL----A 295
YS+ K Y G+P+ G LA++L+ S+ + K + D+ K+ G +++ L A
Sbjct: 370 YSWMKAPRYMGEPMETGPLAQVLIAYSQGHPKVKAVTDAVLAKLGVGPEALFSTLGRTAA 429
Query: 296 QAIELVYALEVASKIAEEL---ISEGIEKSTCEPSE--KSGEGIGYVEAPRGVLVHHYRI 350
+ IE E + +E I++G + C P E K EG+G+V APRG L H RI
Sbjct: 430 RGIETAVIAEYVGVMLQEYKDNIAKG-DNVICAPWEMPKQAEGVGFVNAPRGGLSHWIRI 488
Query: 351 ENERIVWSNTITPTAFNQG-MMEKSLLEDARNMFGKESETTLKSKLE--EVVRAFDPCIS 407
E+ +I + P+ + G +K+ + K +E V +FDPCI+
Sbjct: 489 EDGKIGNFQLVVPSTWTLGPRCDKNNVSPVEASLIGTPVADAKRPVEILRTVHSFDPCIA 548
Query: 408 CSVHVV 413
C VHV+
Sbjct: 549 CGVHVI 554
Score = 69.9 bits (168), Expect = 5e-11
Identities = 35/108 (32%), Positives = 58/108 (53%), Gaps = 1/108 (0%)
Query: 6 IELDEFTRVEGIGKAEIVIENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICAI 65
I +D TR+EG + E+ +ENG VK+A R E++ GR D R C +
Sbjct: 25 IVVDPVTRIEGHLRIEVEVENGKVKNAYSSSTLF-RGLEIILKGRDPRDAQHFTQRTCGV 83
Query: 66 CYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLL 113
C +H++AS R ++NA+G+ + ++ +R L L + + H +H Y L
Sbjct: 84 CTYTHALASTRCVDNAVGVHIPKNATYIRNLVLGAQYLHDHIVHFYHL 131
>pdb|1H2R|L Chain L, Three-Dimensional Structure Of Ni-Fe Hydrogenase From
Desulfivibrio Vulgaris Miyazaki F In The Reduced Form At
1.4 A Resolution
Length = 534
Score = 69.9 bits (168), Expect = 5e-11
Identities = 35/108 (32%), Positives = 58/108 (53%), Gaps = 1/108 (0%)
Query: 6 IELDEFTRVEGIGKAEIVIENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICAI 65
I +D TR+EG + E+ +ENG VK+A R E++ GR D R C +
Sbjct: 7 IVVDPVTRIEGHLRIEVEVENGKVKNAYSSSTLF-RGLEIILKGRDPRDAQHFTQRTCGV 65
Query: 66 CYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLL 113
C +H++AS R ++NA+G+ + ++ +R L L + + H +H Y L
Sbjct: 66 CTYTHALASTRCVDNAVGVHIPKNATYIRNLVLGAQYLHDHIVHFYHL 113
Score = 69.1 bits (166), Expect = 9e-11
Identities = 55/184 (29%), Positives = 88/184 (46%), Gaps = 14/184 (7%)
Query: 241 YSFAKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIKSG-HVIYNNL----A 295
YS+ K Y G+P+ G LA++L+ S+ + K + D+ K+ G +++ L A
Sbjct: 352 YSWMKAPRYMGEPMETGPLAQVLIAYSQGHPKVKAVTDAVLAKLGVGPEALFSTLGRTAA 411
Query: 296 QAIELVYALEVASKIAEEL---ISEGIEKSTCEPSE--KSGEGIGYVEAPRGVLVHHYRI 350
+ IE E + +E I++G + C P E K EG+G+V APRG L H RI
Sbjct: 412 RGIETAVIAEYVGVMLQEYKDNIAKG-DNVICAPWEMPKQAEGVGFVNAPRGGLSHWIRI 470
Query: 351 ENERIVWSNTITPTAFNQG-MMEKSLLEDARNMFGKESETTLKSKLE--EVVRAFDPCIS 407
E+ +I + P+ + G +K+ + K +E V +FDPCI+
Sbjct: 471 EDGKIGNFQLVVPSTWTLGPRCDKNNVSPVEASLIGTPVADAKRPVEILRTVHSFDPCIA 530
Query: 408 CSVH 411
C VH
Sbjct: 531 CGVH 534
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.318 0.137 0.389
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 147509511
Number of Sequences: 2977
Number of extensions: 6036703
Number of successful extensions: 15528
Number of sequences better than 1.0e-10: 38
Number of HSP's better than 0.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 28
Number of HSP's that attempted gapping in prelim test: 15400
Number of HSP's gapped (non-prelim): 56
length of query: 415
length of database: 189,106,746
effective HSP length: 58
effective length of query: 357
effective length of database: 154,394,500
effective search space: 55118836500
effective search space used: 55118836500
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 166 (69.1 bits)