BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0648 (CMO) DE:tungsten-containing aldehyde ferredoxin
oxidoreductase COFACTOR MODIFYING PROTEIN
(373 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||D75071 tungsten-containing aldehyde ferredoxin oxidoreducta... 781 0.0
pir||D71094 probable cofactor modifying protein - Pyrococcus hor... 713 0.0
sp|Q51741|CMO_PYRFU TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXID... 492 e-138
pir||E72327 astB/chuR-related protein - Thermotoga maritima (str... 80 6e-14
pir||F71132 probable nitrite reductase - Pyrococcus horikoshii >... 75 1e-12
pir||C75076 heme biosynthesis protein related PAB1702 - Pyrococc... 70 5e-11
>pir||D75071 tungsten-containing aldehyde ferredoxin oxidoreductase cofactor
modifying protein PAB0648 - Pyrococcus abyssi (strain
Orsay) >gi|5458385|emb|CAB49873.1| (AJ248286)
tungsten-containing aldehyde ferredoxin oxidoreductase
COFACTOR MODIFYING PROTEIN [Pyrococcus abyssi]
Length = 373
Score = 781 bits (1995), Expect = 0.0
Identities = 373/373 (100%), Positives = 373/373 (100%)
Query: 1 MEFQLWGAKVSIEPKPDLKYLYLEITNRCNLKCEMCFKQYWEDEEGDMDWELFLKVLGDA 60
MEFQLWGAKVSIEPKPDLKYLYLEITNRCNLKCEMCFKQYWEDEEGDMDWELFLKVLGDA
Sbjct: 1 MEFQLWGAKVSIEPKPDLKYLYLEITNRCNLKCEMCFKQYWEDEEGDMDWELFLKVLGDA 60
Query: 61 EELPELKMIYFGGIGEPSVHPRFMDMVREVKERGFRLGMSSNGVLLNDKILEEFVKLGVD 120
EELPELKMIYFGGIGEPSVHPRFMDMVREVKERGFRLGMSSNGVLLNDKILEEFVKLGVD
Sbjct: 61 EELPELKMIYFGGIGEPSVHPRFMDMVREVKERGFRLGMSSNGVLLNDKILEEFVKLGVD 120
Query: 121 VIYFSMDAIPTGENLIKLGHVTSKIVENRIKKLVELKEKYGSEKPTIGVEVVATKENYKE 180
VIYFSMDAIPTGENLIKLGHVTSKIVENRIKKLVELKEKYGSEKPTIGVEVVATKENYKE
Sbjct: 121 VIYFSMDAIPTGENLIKLGHVTSKIVENRIKKLVELKEKYGSEKPTIGVEVVATKENYKE 180
Query: 181 LPDMALYLRKLGVDSMLISNLIPLSKEHSHLILYDGSVDMTPIVNKLHSIAEGGPFMRIA 240
LPDMALYLRKLGVDSMLISNLIPLSKEHSHLILYDGSVDMTPIVNKLHSIAEGGPFMRIA
Sbjct: 181 LPDMALYLRKLGVDSMLISNLIPLSKEHSHLILYDGSVDMTPIVNKLHSIAEGGPFMRIA 240
Query: 241 EFKLRTERYCDFVENNVTVVRWDGEVFPCYRFLHTYPEVIFGREKKVIAHSFGNVREKSL 300
EFKLRTERYCDFVENNVTVVRWDGEVFPCYRFLHTYPEVIFGREKKVIAHSFGNVREKSL
Sbjct: 241 EFKLRTERYCDFVENNVTVVRWDGEVFPCYRFLHTYPEVIFGREKKVIAHSFGNVREKSL 300
Query: 301 KEIWMSEEYVRFRFIVKASAYPSCIDCPLNTSCSFVLDTEQDCWGTSPSCGDCLWSRRIV 360
KEIWMSEEYVRFRFIVKASAYPSCIDCPLNTSCSFVLDTEQDCWGTSPSCGDCLWSRRIV
Sbjct: 301 KEIWMSEEYVRFRFIVKASAYPSCIDCPLNTSCSFVLDTEQDCWGTSPSCGDCLWSRRIV 360
Query: 361 LCPIPYEYMGKFL 373
LCPIPYEYMGKFL
Sbjct: 361 LCPIPYEYMGKFL 373
>pir||D71094 probable cofactor modifying protein - Pyrococcus horikoshii
>gi|3257431|dbj|BAA30114.1| (AP000004) 373aa long
hypothetical cofactor modifying protein [Pyrococcus
horikoshii]
Length = 373
Score = 713 bits (1821), Expect = 0.0
Identities = 328/373 (87%), Positives = 356/373 (94%)
Query: 1 MEFQLWGAKVSIEPKPDLKYLYLEITNRCNLKCEMCFKQYWEDEEGDMDWELFLKVLGDA 60
MEFQLWGAK+ IEPKPDL+YLYLEITNRCNLKCEMCFKQYWED+EGDMDW+LFLK+L DA
Sbjct: 1 MEFQLWGAKIVIEPKPDLRYLYLEITNRCNLKCEMCFKQYWEDKEGDMDWDLFLKILDDA 60
Query: 61 EELPELKMIYFGGIGEPSVHPRFMDMVREVKERGFRLGMSSNGVLLNDKILEEFVKLGVD 120
+E PELKMIYFGGIGEPSVHPRFMDMVREVK RGFRLGMS+NGVLL DKILEEFVKLGVD
Sbjct: 61 KEFPELKMIYFGGIGEPSVHPRFMDMVREVKRRGFRLGMSTNGVLLEDKILEEFVKLGVD 120
Query: 121 VIYFSMDAIPTGENLIKLGHVTSKIVENRIKKLVELKEKYGSEKPTIGVEVVATKENYKE 180
V+YFSMDAIPT N++ LGH+TSK+VE+RIKKLVELK++Y +EKPTIGVEVVATKENYK+
Sbjct: 121 VVYFSMDAIPTPSNIVTLGHITSKVVESRIKKLVELKKRYKTEKPTIGVEVVATKENYKQ 180
Query: 181 LPDMALYLRKLGVDSMLISNLIPLSKEHSHLILYDGSVDMTPIVNKLHSIAEGGPFMRIA 240
LP+MA+YLR LGVDSMLISNLIP++KEHS I+YDGSVDMTPIVN+LH IAEGGPFMRIA
Sbjct: 181 LPEMAIYLRNLGVDSMLISNLIPMTKEHSKAIVYDGSVDMTPIVNELHQIAEGGPFMRIA 240
Query: 241 EFKLRTERYCDFVENNVTVVRWDGEVFPCYRFLHTYPEVIFGREKKVIAHSFGNVREKSL 300
EFKLRT RYCDF+ENNVTVVRWDGEVFPCYRFLHTYPEVIFGREKKVIAHSFGNV+EKSL
Sbjct: 241 EFKLRTNRYCDFIENNVTVVRWDGEVFPCYRFLHTYPEVIFGREKKVIAHSFGNVKEKSL 300
Query: 301 KEIWMSEEYVRFRFIVKASAYPSCIDCPLNTSCSFVLDTEQDCWGTSPSCGDCLWSRRIV 360
K+IWMSEEYVRFRF+VKASAYPSCIDCPLNTSCSF LDT QDCWGTSPSCGDCLWSR IV
Sbjct: 301 KDIWMSEEYVRFRFLVKASAYPSCIDCPLNTSCSFALDTTQDCWGTSPSCGDCLWSRGIV 360
Query: 361 LCPIPYEYMGKFL 373
LCPIP+EYMGKFL
Sbjct: 361 LCPIPFEYMGKFL 373
>sp|Q51741|CMO_PYRFU TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE COFACTOR
MODIFYING PROTEIN >gi|736276|emb|CAA56172.1| (X79777)
cofactor modifying protein [Pyrococcus furiosus]
Length = 357
Score = 492 bits (1254), Expect = e-138
Identities = 226/357 (63%), Positives = 281/357 (78%), Gaps = 1/357 (0%)
Query: 18 LKYLYLEITNRCNLKCEMCFKQYWEDEEGDMDWELFLKVLGDAEELPELKM-IYFGGIGE 76
+KYLYLEIT+RCNL+CEMCFKQ+WEDEEGDMD++LFLK+L E+ K I IGE
Sbjct: 1 MKYLYLEITSRCNLRCEMCFKQHWEDEEGDMDYDLFLKILRRCREVSRAKDDILRRSIGE 60
Query: 77 PSVHPRFMDMVREVKERGFRLGMSSNGVLLNDKILEEFVKLGVDVIYFSMDAIPTGENLI 136
P+VHPRFMDMVREVK+RGF LG+S+NG LL D++L+E +LGVD++YFSMD +PT +N++
Sbjct: 61 PTVHPRFMDMVREVKKRGFALGISTNGTLLTDEMLKELAELGVDLVYFSMDVLPTAQNVV 120
Query: 137 KLGHVTSKIVENRIKKLVELKEKYGSEKPTIGVEVVATKENYKELPDMALYLRKLGVDSM 196
LGH+ + + +IKKLV+ +E+ G+ KP+IGVEVV TKENYK+LP++A YL LGVD+M
Sbjct: 121 TLGHILAGVTAEKIKKLVKYREEVGTHKPSIGVEVVVTKENYKQLPELARYLLNLGVDAM 180
Query: 197 LISNLIPLSKEHSHLILYDGSVDMTPIVNKLHSIAEGGPFMRIAEFKLRTERYCDFVENN 256
L+SNL+PL+ E + I+YDGS+DMTPIVN+L+ IA G ++++ F+L+TER CDF EN
Sbjct: 181 LVSNLLPLTPEQVNDIVYDGSIDMTPIVNELYKIAHNGLYIKLPYFELKTERVCDFDENK 240
Query: 257 VTVVRWDGEVFPCYRFLHTYPEVIFGREKKVIAHSFGNVREKSLKEIWMSEEYVRFRFIV 316
V V+RWDGEV PCYRFLHTY E IFGREKKV +SFGNVREKSL +IW SE Y FRFI
Sbjct: 241 VAVIRWDGEVVPCYRFLHTYREYIFGREKKVNTYSFGNVREKSLADIWTSERYTWFRFIT 300
Query: 317 KASAYPSCIDCPLNTSCSFVLDTEQDCWGTSPSCGDCLWSRRIVLCPIPYEYMGKFL 373
K YPSC DC L +C FV T+ DCWG PSC DCLWSRRIV+CPIP GKFL
Sbjct: 301 KNYLYPSCTDCSLRDACDFVKTTDIDCWGNEPSCADCLWSRRIVMCPIPQYNFGKFL 357
>pir||E72327 astB/chuR-related protein - Thermotoga maritima (strain MSB8)
>gi|4981357|gb|AAD35907.1|AE001750_1 (AE001750)
astB/chuR-related protein [Thermotoga maritima]
Length = 323
Score = 79.6 bits (193), Expect = 6e-14
Identities = 78/318 (24%), Positives = 139/318 (43%), Gaps = 43/318 (13%)
Query: 24 EITNRCNLKCEMCFKQYWEDEEGDMDWELFLKVLGDAEELPELKMIYFGGIGEPSVHPRF 83
E+T RCN +C+ C+ + + ++ +E +++ D +EL + GG EP +HP+
Sbjct: 11 ELTLRCNFRCKHCYCEAGKPHLEELSFEEIKELILDMKELGTWALDIVGG--EPLLHPQI 68
Query: 84 MDMVREVKERGFRLGMSSNGVLLNDKILEEFVKLGVDVIYFSMDAIPTGENLIKLGHVTS 143
+D++ KE G RL +++NG L +++++ K DV+ G +L T+
Sbjct: 69 LDILAFGKEVGQRLMINTNGSLATKEMVQKIKKANPDVL--------IGVSLEGPDPETN 120
Query: 144 KIVE--NRIKKLVELKEKYGSEKPTIGVEVVATKENYKELPDMALYLRKLGVDSMLISNL 201
V K+ V+ + + E + + V K N+++ DM +LGV+++ +
Sbjct: 121 DFVRGTGNFKRAVQGIKNFVDEGFQVTILNVINKRNWRKFEDMVKLALELGVNALYVDRF 180
Query: 202 IPLSKEHSHLILYDGSVDMTP------IVNKLHSIAEGGPFMRIAEFKLRTERYCDFVEN 255
IP+ + +++ +DM P I + L I + + + + C
Sbjct: 181 IPVGRG----MIHARELDMNPEEWRVAIKHVLGVIENYKNHLTFYVEESISGKPCSAGIT 236
Query: 256 NVTVVRWDGEVFPCYRFLHTYPEVIFGREKKVIAHSFGNVREKSLKEIWMSEEYVRFRFI 315
+ +V+ DG V PC F R +K GNVREK EIW EY
Sbjct: 237 HASVLA-DGTVVPCGHF----------RYRK--EFYMGNVREKKFSEIW--HEY------ 275
Query: 316 VKASAYPSCIDCPLNTSC 333
+ SC CP+ C
Sbjct: 276 TPIPSPASCQQCPILNEC 293
>pir||F71132 probable nitrite reductase - Pyrococcus horikoshii
>gi|3257237|dbj|BAA29920.1| (AP000003) 552aa long
hypothetical nitrite reductase [Pyrococcus horikoshii]
Length = 552
Score = 74.9 bits (181), Expect = 1e-12
Identities = 49/198 (24%), Positives = 94/198 (46%), Gaps = 14/198 (7%)
Query: 26 TNRCNLKCEMCFKQYWEDEEGDMDWELFLKVLGDAEELPELKMIYFGGIGEPSVHPRFMD 85
TN CN +C+ C+++ + ++ E L ++ + + GG EP++HP F+
Sbjct: 156 TNMCNFRCKHCYQRADKPLPSELSLEEKLMLVDQLDRAGVAAVALSGG--EPTIHPHFLR 213
Query: 86 MVREVKERGFRLGMSSNGVLLNDK-ILEEFVKLGVDVIYFSMDAIPTGENLIKLGHVTSK 144
+V+E+ RG +++NG DK LEE VK G+ + S+D+ + H +
Sbjct: 214 IVKELSSRGIHTSVATNGWTFADKEKLEEAVKAGLKYVEISVDSAKPEK------HDEFR 267
Query: 145 IVENRIKKLVELKEKYGSEKPTIGVEVVATKENYKELPDMALYLRKLGVDSMLISNLIPL 204
+ ++ ++ E + G+ V+ KE Y+E+ D+ +GV ++ NL+P
Sbjct: 268 GIPGAWERAIKALENAVEIGVSHGMAVIMDKETYQEMDDILDLAESIGVKRVIFFNLVPT 327
Query: 205 SKEHSHLILYDGSVDMTP 222
+ + VD+TP
Sbjct: 328 GRAEDMV-----KVDLTP 340
>pir||C75076 heme biosynthesis protein related PAB1702 - Pyrococcus abyssi
(strain Orsay) >gi|5458424|emb|CAB49912.1| (AJ248286)
HEME BIOSYNTHESIS PROTEIN related [Pyrococcus abyssi]
Length = 441
Score = 69.9 bits (168), Expect = 5e-11
Identities = 78/324 (24%), Positives = 134/324 (41%), Gaps = 40/324 (12%)
Query: 23 LEITNRCNLKCEMCFKQYWEDEEGDMDWELFLKVLGDAEELPELKMIYFGGIGEPSVHPR 82
+EIT+RCNLKCE C+ + + E+ K++ D E+L ++ G GEP++HP
Sbjct: 115 VEITHRCNLKCEHCYVG-TRNNPATLSLEVIKKLIHDMEKLGCYQLAIGG--GEPTLHPN 171
Query: 83 FMDMVREVKERGFRLGMSSNGVLLNDKILEEFV--KLGVDVIYFSMDAIPTGENLIKLGH 140
F ++ + + + +NG + LE++ K + S+D ++
Sbjct: 172 FEKILEIIHKSKIFAHIVTNGTTNLSEYLEKYANKKKRSFEVTISIDGPKEIHEKVRKSV 231
Query: 141 VTSKIVENRIKKLVELKEKYGSEKPTIGVEVVATKENYKELPDMALYLRKLGVDSMLISN 200
+I+ N IKKL EL KP I + + + YK +P + L+ L + + +
Sbjct: 232 KFEEII-NNIKKLAEL-----GWKPQI--QTTVSLKTYKYIPKLLKELKDLPISAWAVKM 283
Query: 201 LIPLSKEHSHLILYDGSVDMTPIVNKLHSIAEGGPFMRIAEF---------------KLR 245
P+ SH ++ S + + +++ E RI EF K R
Sbjct: 284 EYPVGNARSHANIFPSSTEFVKMTSEIWEWWEESNLKRI-EFVEDLGYFPPAIKKPAKKR 342
Query: 246 TERYCDFVENNVTVVRWDGEVFPCYRFLHTYPEVIFGREKKVIAHSFGNVREKSLKEIWM 305
C VT+ +G + PC T ++ G K + GN+ SL E+W
Sbjct: 343 AYYLCSAGVTQVTIDA-EGNITPC-----TLIQITDGSTKYIA----GNLYYDSLIEVWH 392
Query: 306 SEEYVRFRFIVKASAYPSCIDCPL 329
+ V + F + SC C L
Sbjct: 393 TSN-VLWEFRLMWPENESCARCGL 415
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.322 0.141 0.438
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 147810333
Number of Sequences: 2977
Number of extensions: 6483993
Number of successful extensions: 14474
Number of sequences better than 1.0e-10: 6
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 14462
Number of HSP's gapped (non-prelim): 7
length of query: 373
length of database: 189,106,746
effective HSP length: 51
effective length of query: 322
effective length of database: 158,583,909
effective search space: 51064018698
effective search space used: 51064018698
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 166 (69.1 bits)