BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0675 (PAB0675) DE:MOLYBDENUM FORMYLMETHANOFURAN
DEHYDROGENASE SUBUNIT FMDE related
(231 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||H75076 molybdenum formylmethanofuran dehydrogenas E chain f... 467 e-131
pir||H71067 hypothetical protein PH1238 - Pyrococcus horikoshii ... 355 3e-97
gi|11498045 conserved hypothetical protein [Archaeoglobus fulgid... 242 3e-63
gi|11497794 tungsten formylmethanofuran dehydrogenase, subunit E... 100 2e-20
pir||G69045 conserved hypothetical protein MTH1345 - Methanobact... 94 1e-18
pir||B75580 conserved hypothetical protein - Deinococcus radiodu... 79 5e-14
emb|CAC12236.1| (AL445066) conserved hypothetical protein [Therm... 78 1e-13
>pir||H75076 molybdenum formylmethanofuran dehydrogenas E chain fmde related
PAB0675 - Pyrococcus abyssi (strain Orsay)
>gi|5458429|emb|CAB49917.1| (AJ248286) MOLYBDENUM
FORMYLMETHANOFURAN DEHYDROGENAS E SUBUNIT FMDE related
[Pyrococcus abyssi]
Length = 231
Score = 467 bits (1190), Expect = e-131
Identities = 231/231 (100%), Positives = 231/231 (100%)
Query: 1 MGKLNELVERGDAEGILNYAREFHGHVCPYLALGIRASLIAMEELGVGRLDYSGSVDESI 60
MGKLNELVERGDAEGILNYAREFHGHVCPYLALGIRASLIAMEELGVGRLDYSGSVDESI
Sbjct: 1 MGKLNELVERGDAEGILNYAREFHGHVCPYLALGIRASLIAMEELGVGRLDYSGSVDESI 60
Query: 61 LAIVETNSCFTDGVQVTTGCTLGNNSLIYIDVGKTALTLVKRGSWEGVRVYVDAEKLRSY 120
LAIVETNSCFTDGVQVTTGCTLGNNSLIYIDVGKTALTLVKRGSWEGVRVYVDAEKLRSY
Sbjct: 61 LAIVETNSCFTDGVQVTTGCTLGNNSLIYIDVGKTALTLVKRGSWEGVRVYVDAEKLRSY 120
Query: 121 YPPEATELFEKVVKERRGTSEEREKLRELWEELGRKFLYLPRKVFEIKRIKVPPIEQAPI 180
YPPEATELFEKVVKERRGTSEEREKLRELWEELGRKFLYLPRKVFEIKRIKVPPIEQAPI
Sbjct: 121 YPPEATELFEKVVKERRGTSEEREKLRELWEELGRKFLYLPRKVFEIKRIKVPPIEQAPI 180
Query: 181 FESVRCSECGELVMSPKVVYVNEKPLCKVCANKKILAVIGRGIVELEGFRV 231
FESVRCSECGELVMSPKVVYVNEKPLCKVCANKKILAVIGRGIVELEGFRV
Sbjct: 181 FESVRCSECGELVMSPKVVYVNEKPLCKVCANKKILAVIGRGIVELEGFRV 231
>pir||H71067 hypothetical protein PH1238 - Pyrococcus horikoshii
>gi|3257655|dbj|BAA30338.1| (AP000005) 231aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 231
Score = 355 bits (901), Expect = 3e-97
Identities = 169/227 (74%), Positives = 191/227 (83%)
Query: 1 MGKLNELVERGDAEGILNYAREFHGHVCPYLALGIRASLIAMEELGVGRLDYSGSVDESI 60
M LN ++ + IL YA+ FHGH+CPYLALGIR+SLIAMEEL V RL SVDESI
Sbjct: 1 MENLNLAIKEKKFDVILEYAKVFHGHICPYLALGIRSSLIAMEELSVNRLGLEESVDESI 60
Query: 61 LAIVETNSCFTDGVQVTTGCTLGNNSLIYIDVGKTALTLVKRGSWEGVRVYVDAEKLRSY 120
LAIVETNSCF DGVQVTTGCT GNNSLI++D GKTALTLVKRG+WEGVRVYVDAEKL+ Y
Sbjct: 61 LAIVETNSCFADGVQVTTGCTFGNNSLIFLDTGKTALTLVKRGNWEGVRVYVDAEKLKDY 120
Query: 121 YPPEATELFEKVVKERRGTSEEREKLRELWEELGRKFLYLPRKVFEIKRIKVPPIEQAPI 180
YPP A ELFEKV+K R GT EE+ KL +LWEE GRKFLYLP+++FE+K++KV PIEQAPI
Sbjct: 121 YPPGAIELFEKVIKRREGTPEEKRKLSKLWEETGRKFLYLPKEIFEVKKVKVSPIEQAPI 180
Query: 181 FESVRCSECGELVMSPKVVYVNEKPLCKVCANKKILAVIGRGIVELE 227
FESVRCS CGELVM+PK VY+N KP CKVCA K+IL VIGRGIVELE
Sbjct: 181 FESVRCSRCGELVMAPKAVYINGKPFCKVCAGKEILGVIGRGIVELE 227
>gi|11498045 conserved hypothetical protein [Archaeoglobus fulgidus]
>gi|3915359|sp|O29816|Y433_ARCFU HYPOTHETICAL PROTEIN
AF0433 >gi|7483009|pir||A69304 conserved hypothetical
protein AF0433 - Archaeoglobus fulgidus
>gi|2650198|gb|AAB90804.1| (AE001075) conserved
hypothetical protein [Archaeoglobus fulgidus]
Length = 366
Score = 242 bits (611), Expect = 3e-63
Identities = 119/213 (55%), Positives = 157/213 (72%), Gaps = 6/213 (2%)
Query: 16 ILNYAREFHGHVCPYLALGIRASLIAMEELGVGRLDYSGSVDESILAIVETNSCFTDGVQ 75
+L A+EFHGH+CP++ALG+R S+IAME+LGV D SV E ILAIVE N+C TDGVQ
Sbjct: 154 LLLRAKEFHGHICPFVALGVRMSVIAMEKLGVEE-DAMASVGEDILAIVECNNCLTDGVQ 212
Query: 76 VTTGCTLGNNSLIYIDVGKTALTLVKRGSWEGVRVYVDAEKLRSYYPPEATELFEKVVKE 135
V TGCTLGNNSLIY+D+GK ALT+V+R W+GVRVYV+AE +R Y+ EA ELF KV+
Sbjct: 213 VATGCTLGNNSLIYLDLGKNALTIVRRKDWKGVRVYVNAENVRKYFSKEALELFNKVIVR 272
Query: 136 RRGTSEEREKLRELWEELGRKFLYLPRKVFEIKRIKVPPIEQAPIFESVRCSECGELVMS 195
R G+ E+ ++L E WEE G K L +P + F+++ ++V PIE+APIFE+ RC +CGEL M+
Sbjct: 273 REGSEEDAKRLSEFWEETGWKMLEIPEEEFKVEFVEVQPIERAPIFENKRCEKCGELAMA 332
Query: 196 PKVVYVNEKPLCKVCANKKILAVIGRGIVELEG 228
+V + LC CA AV+GRGIV+ +G
Sbjct: 333 TRV----KDGLCLRCAG-SYYAVVGRGIVKFDG 360
>gi|11497794 tungsten formylmethanofuran dehydrogenase, subunit E (fwdE)
[Archaeoglobus fulgidus] >gi|7450958|pir||A69272
tungsten formylmethanofuran dehydrogenase, subunit E
(fwdE) homolog - Archaeoglobus fulgidus
>gi|2650468|gb|AAB91055.1| (AE001094) tungsten
formylmethanofuran dehydrogenase, subunit E (fwdE)
[Archaeoglobus fulgidus]
Length = 189
Score = 100 bits (246), Expect = 2e-20
Identities = 70/205 (34%), Positives = 108/205 (52%), Gaps = 24/205 (11%)
Query: 12 DAEGILNYAREFHGHVCPYLALGIRASLIAMEELGVGRLDYSGSVDESILAIVETNSCFT 71
D +L +A + H H+ P +ALGI+ + IA E+LGV ++ G + ++ I ET C
Sbjct: 2 DRNEMLEFAAKLHSHLSPGVALGIKMAQIAYEKLGV---NFRG---KGLVGIAETAMCLP 55
Query: 72 DGVQVTTGCTLGNNSLIYIDVGKTALTLVKRGSWEGVRVYVDAEKLRSYYPPEATELFEK 131
D +QV G T GN +LI D GK AL +V+ + EG RV + E + +EL +K
Sbjct: 56 DALQVVAGTTPGNRNLIVKDYGKLALAIVRFDTREGYRVTIKKEAAKK------SELIKK 109
Query: 132 VVKERRGTSEEREKLRELWEELGRKFLYLPRKVFEIKRIKVP-PI--EQAPIFESVRCSE 188
+ ++E EK L +FL L + FE++ I++ P+ + PI V C +
Sbjct: 110 FLYRLGKLNKEEEK------RLAEEFLNLEPEYFEVREIRLTIPVGGTKEPI---VECEK 160
Query: 189 CGELVMSPKVVYVNEKPLCKVCANK 213
CGEL S V V+ + +C +CA K
Sbjct: 161 CGELQPSDYVAEVDGRMVCFMCAGK 185
>pir||G69045 conserved hypothetical protein MTH1345 - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2622451|gb|AAB85822.1| (AE000897) conserved protein
[Methanobacterium thermoautotrophicum]
Length = 198
Score = 94.0 bits (230), Expect = 1e-18
Identities = 62/188 (32%), Positives = 89/188 (46%), Gaps = 28/188 (14%)
Query: 23 FHGHVCPYLALGIRASLIAMEELGVGRLDYSGSVDESILAIVETNSCFTDGVQVTTGCTL 82
FHGH C ALG + I E G S DE I+A+VE +SC D +Q TGCT
Sbjct: 36 FHGHSCAGTALGYKVGEIVAERFG-------RSEDEEIVAVVENDSCSIDAIQFMTGCTF 88
Query: 83 GNNSLIYIDVGKTALTLVKRGSWEGVRVYVDAEKLRSYYPPEATELFEKVVKERRGTSEE 142
G +LI+ D GK T R + +G+R+ S P + E V +R +E
Sbjct: 89 GKGNLIFRDYGKHVYTFFNRKTGDGIRI--------SLKKPMDQLMKELGVTDR---AEL 137
Query: 143 REKLRELWEELGRKFLYLPRKVFEIKRIKVPPIEQAPIFESVRCSECGELVMSPKVVYVN 202
EK+ E+ P +FE++ + P E+A I+ S+ C+ECGE V + N
Sbjct: 138 TEKILEM----------DPHDLFEVRDVSEKPPEEARIYGSIICAECGEPVSEHRARVKN 187
Query: 203 EKPLCKVC 210
+ +C C
Sbjct: 188 GRMVCIPC 195
>pir||B75580 conserved hypothetical protein - Deinococcus radiodurans (strain
R1) >gi|6460672|gb|AAF12377.1|AE001863_2 (AE001863)
conserved hypothetical protein [Deinococcus radiodurans]
Length = 198
Score = 78.8 bits (191), Expect = 5e-14
Identities = 62/208 (29%), Positives = 97/208 (45%), Gaps = 27/208 (12%)
Query: 16 ILNYAREFHGHVCPYLALGIRASLIAMEELGVGRLDYSGSVDESILAIVETNSCFTDGVQ 75
+L + HGH+CP LG+R +L+A E LG LD+ S D+ L VET+ CF DGV
Sbjct: 14 LLARSSALHGHLCPRQVLGVRLALLAGETLG---LDFPRS-DKRALVFVETDGCFADGVS 69
Query: 76 VTTGCTLGNNSLIYIDVGKTALTLVKRGSWEGVRVYVDAEKLRSYYPPEATELFEKVVKE 135
V +GC LG +L +D GK A +V S VRV T+L +V
Sbjct: 70 VGSGCWLGRRTLRLVDHGKVAAAVVDTRSGRAVRV------------RPQTDLRARV--- 114
Query: 136 RRGTSEEREKLRELWEELGRKFLYLPRKVFEIK----RIKVPPIEQAPIFESVRCSECGE 191
R G E + + + L + +F+++ R + + A +V C CGE
Sbjct: 115 RAGRPEGQTRYQAY---LDAYQTWPDADLFDVQPVDLRFDLAALVSAAGKRAV-CDRCGE 170
Query: 192 LVMSPKVVYVNEKPLCKVCANKKILAVI 219
+++ + V ++ LC+ CA ++
Sbjct: 171 EIVNEREVVQGDETLCRGCAGDSYFTLL 198
>emb|CAC12236.1| (AL445066) conserved hypothetical protein [Thermoplasma
acidophilum]
Length = 203
Score = 77.6 bits (188), Expect = 1e-13
Identities = 59/198 (29%), Positives = 90/198 (44%), Gaps = 12/198 (6%)
Query: 15 GILNYAREFHGHVCPYLALGIRASLIAMEELGVGRLDYSGSVDESILAIVETNSCFTDGV 74
GI +A EFHGH CPY+ +G RA A++ G+ + + S ++ + N CF DG
Sbjct: 7 GIPEWAFEFHGHKCPYMPMGYRAGSYALKIAGLEKEKDHRTYLLSEMSPEDMNGCFNDGA 66
Query: 75 QVTTGCTLGNNSLIYIDVGKTALTLVKRGSWEGVRVYVDAEKLRSYYPPEATELFEKVVK 134
Q TGCT G + GK AL L + G + +RV+V R+ + E + +
Sbjct: 67 QAATGCTYGKGLFSLLGYGKLALILYRPGR-KAIRVHV-----RNSFMDELSTRASDFFR 120
Query: 135 ERRGTSEEREKLRELWEELGRKFLYLP-RKVFEIKRIKVPPIEQAPIFES---VRCSECG 190
R+ E E + + L ++FE + I E P+ ++ VRC CG
Sbjct: 121 YRKQGYEPSEIPAGAIDPVLEWISSLEDEEIFEYREIDGFTFE--PVKKNGAKVRCDVCG 178
Query: 191 ELVMSPKVVYVNEKPLCK 208
E +N KP+CK
Sbjct: 179 EYTYEADAKLLNGKPVCK 196
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.320 0.140 0.408
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84593743
Number of Sequences: 2977
Number of extensions: 3390293
Number of successful extensions: 10120
Number of sequences better than 1.0e-10: 7
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 10106
Number of HSP's gapped (non-prelim): 10
length of query: 231
length of database: 189,106,746
effective HSP length: 54
effective length of query: 177
effective length of database: 156,788,448
effective search space: 27751555296
effective search space used: 27751555296
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 164 (68.3 bits)