BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0703 (PAB0703) DE:HYDROXYACYLGLUTATHIONE HYDROLASE related
(EC 3.1.2.6) (GLYOXALASE II)
(222 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||B75083 probable hydroxyacylglutathione hydrolase (EC 3.1.2.... 458 e-128
pir||G71064 hypothetical protein PH1213 - Pyrococcus horikoshii ... 378 e-104
gi|11499337 conserved hypothetical protein [Archaeoglobus fulgid... 119 2e-26
pir||D69036 conserved hypothetical protein MTH1267 - Methanobact... 117 1e-25
gi|11498183 conserved hypothetical protein [Archaeoglobus fulgid... 107 1e-22
sp|Q58298|Y888_METJA HYPOTHETICAL PROTEIN MJ0888 >gi|2128038|pir... 93 3e-18
gb|AAK43233.1| Conserved hypothetical protein [Sulfolobus solfat... 87 2e-16
gi|11499983 conserved hypothetical protein [Archaeoglobus fulgid... 87 2e-16
gi|11498145 conserved hypothetical protein [Archaeoglobus fulgid... 83 2e-15
sp|O67893|YL35_AQUAE HYPOTHETICAL PROTEIN AQ_2135 >gi|7446954|pi... 83 2e-15
sp|P54501|YQGX_BACSU HYPOTHETICAL 23.2 KDA PROTEIN IN SODA-COMGA... 78 1e-13
pir||F82220 glyoxylase II family protein VC1270 [imported] - Vib... 76 3e-13
pir||D72599 hypothetical protein APE1260 - Aeropyrum pernix (str... 76 3e-13
emb|CAC11806.1| (AL445065) conserved hypothetical protein [Therm... 75 6e-13
dbj|BAB06539.1| (AP001516) unknown conserved protein [Bacillus h... 75 8e-13
gb|AAK42646.1| Conserved hypothetical protein [Sulfolobus solfat... 75 8e-13
sp|P75849|YCBL_ECOLI HYPOTHETICAL 23.8 KDA PROTEIN IN MUKB-ASPC ... 74 1e-12
pir||B81011 glyoxalase II family protein NMB2049 [imported] - Ne... 73 2e-12
pir||A81955 probable hydrolase NMA0387 [imported] - Neisseria me... 73 2e-12
emb|CAC14523.1| (AL446004) possible CGI-83 protein [Leishmania m... 73 2e-12
gb|AAG10453.1|AF279106_15 (AF279106) predicted metallobeta lacta... 73 4e-12
pir||A81353 probable hydrolase Cj0809c [imported] - Campylobacte... 71 1e-11
gb|AAG19667.1| (AE005054) flavoprotein; FprA [Halobacterium sp. ... 69 4e-11
sp|Q49649|YP81_MYCLE HYPOTHETICAL 23.9 KDA PROTEIN MLCB1259.11/B... 68 1e-10
sp|Q57544|YCBL_HAEIN HYPOTHETICAL PROTEIN HI1663 >gi|1074886|pir... 68 1e-10
pir||A75189 hypothetical protein PAB0023 - Pyrococcus abyssi (st... 68 1e-10
>pir||B75083 probable hydroxyacylglutathione hydrolase (EC 3.1.2.6) PAB0703
[imported] - Pyrococcus abyssi (strain Orsay)
>gi|5458479|emb|CAB49967.1| (AJ248286)
HYDROXYACYLGLUTATHIONE HYDROLASE related (EC 3.1.2.6)
(GLYOXALASE II) [Pyrococcus abyssi]
Length = 222
Score = 458 bits (1166), Expect = e-128
Identities = 222/222 (100%), Positives = 222/222 (100%)
Query: 1 MIPIIIKPNILMLEGVNLDSNVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSK 60
MIPIIIKPNILMLEGVNLDSNVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSK
Sbjct: 1 MIPIIIKPNILMLEGVNLDSNVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSK 60
Query: 61 VIIFNTHEHFDHVGGNLVFKEKLKVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHEV 120
VIIFNTHEHFDHVGGNLVFKEKLKVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHEV
Sbjct: 61 VIIFNTHEHFDHVGGNLVFKEKLKVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHEV 120
Query: 121 ELKLEDGDEIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPTGN 180
ELKLEDGDEIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPTGN
Sbjct: 121 ELKLEDGDEIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPTGN 180
Query: 181 EDKIVESLELLKSFDVRLGLPGHGRVIKGWKANLERILRVLK 222
EDKIVESLELLKSFDVRLGLPGHGRVIKGWKANLERILRVLK
Sbjct: 181 EDKIVESLELLKSFDVRLGLPGHGRVIKGWKANLERILRVLK 222
>pir||G71064 hypothetical protein PH1213 - Pyrococcus horikoshii
>gi|3257630|dbj|BAA30313.1| (AP000005) 223aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 223
Score = 378 bits (961), Expect = e-104
Identities = 170/222 (76%), Positives = 200/222 (89%)
Query: 1 MIPIIIKPNILMLEGVNLDSNVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSK 60
MIPIIIKPNILML G+NLDSN+YFLKSK LLIVDTGTGVYWNKYL A EGWL ++
Sbjct: 2 MIPIIIKPNILMLRGLNLDSNIYFLKSKKSLLIVDTGTGVYWNKYLGIAEREGWLRDIKD 61
Query: 61 VIIFNTHEHFDHVGGNLVFKEKLKVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHEV 120
VIIFNTHEHFDHVGGNL+FKEKL+ V+FA+ +LTAKALE+GDDY+ILS+YYGRRY+PH+V
Sbjct: 62 VIIFNTHEHFDHVGGNLIFKEKLRNVKFAAQRLTAKALEDGDDYVILSFYYGRRYEPHDV 121
Query: 121 ELKLEDGDEIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPTGN 180
ELKLEDG++I++G ++L L+HTPGHTRGSSCLYY+EE+IMFTGDTVF GTYGRTDLPTG+
Sbjct: 122 ELKLEDGEKIRIGNIELTLLHTPGHTRGSSCLYYKEEKIMFTGDTVFAGTYGRTDLPTGD 181
Query: 181 EDKIVESLELLKSFDVRLGLPGHGRVIKGWKANLERILRVLK 222
E K++ESLE LK FDVR+GLPGHG++I WK N+E ILRVLK
Sbjct: 182 ESKMIESLEFLKDFDVRIGLPGHGKIINNWKENIEAILRVLK 223
>gi|11499337 conserved hypothetical protein [Archaeoglobus fulgidus]
>gi|7483162|pir||C69468 conserved hypothetical protein
AF1748 - Archaeoglobus fulgidus
>gi|2648803|gb|AAB89502.1| (AE000982) conserved
hypothetical protein [Archaeoglobus fulgidus]
Length = 236
Score = 119 bits (296), Expect = 2e-26
Identities = 75/213 (35%), Positives = 118/213 (55%), Gaps = 5/213 (2%)
Query: 6 IKPNILMLEGVNLDSNVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSKVIIFN 65
I NI ++ L S+ Y + ++ LI D+GT + K L+ E L I+ N
Sbjct: 7 IHNNIYQIKPGRLSSHCYLITAELNALI-DSGTARDFPK-LERELGEIGLNAKDIDIVIN 64
Query: 66 THEHFDHVGGNLVFKEKLKVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHEVELKLE 125
THEHFDH+GGNL F +K ++ +H+ A + GDD +++ +G+R + V + L
Sbjct: 65 THEHFDHIGGNL-FLQKNSII--MAHRHAAVKIIYGDDEVMMCRTHGQRPVGYRVHVWLN 121
Query: 126 DGDEIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPTGNEDKIV 185
+ D + +G V LR++HTPGHT G CLY RI+F+GDT+F + +G+ +
Sbjct: 122 NIDAVDLGGVFLRVMHTPGHTSGCICLYDPRNRILFSGDTLFANGTLSSIYNSGSLGEYF 181
Query: 186 ESLELLKSFDVRLGLPGHGRVIKGWKANLERIL 218
SL +K+ + L LPGHGR+ + ++ER L
Sbjct: 182 NSLRKIKTMKIDLLLPGHGRISGNVEVDIERTL 214
>pir||D69036 conserved hypothetical protein MTH1267 - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2622381|gb|AAB85756.1| (AE000893) conserved protein
[Methanobacterium thermoautotrophicum]
Length = 205
Score = 117 bits (291), Expect = 1e-25
Identities = 78/208 (37%), Positives = 110/208 (52%), Gaps = 18/208 (8%)
Query: 9 NILMLEGVNLDSNVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSKVIIFNTHE 68
+I+ +EG DSN+Y L IVDTGTG+ + L R G + K I+ NTH
Sbjct: 7 DIIAVEGTLFDSNMYILGDT----IVDTGTGMNPDALLARMRGAGVNPSDIKHIV-NTHC 61
Query: 69 HFDHVGGNLVFKEKLKVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHEVELKLEDGD 128
HFDH GGN +F + A H L A AL EGDD ++Y + D +V ++L +GD
Sbjct: 62 HFDHTGGNRLFD-----ADIAIHSLDADALREGDDARTVAYMFSASMDAMDVAVELGEGD 116
Query: 129 EIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFL-GTYGRTDLPTGNEDKIVES 187
+ +IHTPGHT G CLY E + +GDTVF G +GR D+ G+ ++ +S
Sbjct: 117 FVG----DFEVIHTPGHTPGCICLY--EGESLISGDTVFADGGFGRVDV-GGDISELADS 169
Query: 188 LELLKSFDVRLGLPGHGRVIKGWKANLE 215
+ L+ DV PGHG + ++E
Sbjct: 170 IRRLRRLDVEYLFPGHGPWVDNGSRHVE 197
>gi|11498183 conserved hypothetical protein [Archaeoglobus fulgidus]
>gi|7446963|pir||G69321 conserved hypothetical protein
AF0575 - Archaeoglobus fulgidus
>gi|2650049|gb|AAB90665.1| (AE001065) conserved
hypothetical protein [Archaeoglobus fulgidus]
Length = 209
Score = 107 bits (264), Expect = 1e-22
Identities = 74/218 (33%), Positives = 112/218 (50%), Gaps = 35/218 (16%)
Query: 18 LDSNVYFLKSKDELLIVDTGTGVYW-----NKYLDTARNEGWLENVSKVIIFNTHEHFDH 72
L +N Y L + + LI D G + + +Y+D + I TH H+DH
Sbjct: 11 LAANCYLLLDEKKALI-DVGGDIQFILNALKRYIDPRELD---------YIILTHSHYDH 60
Query: 73 VGGNLVFKEKLKVVEFASHKLTAKALEEGDDYIIL------SYYYGRRYDPHEVELKLED 126
FK K+ A HK D+Y +L S+ +G + E ++ LE
Sbjct: 61 AAAAGHFKGIAKI---AIHK---------DEYSLLKAQGFTSFMFGVSFKKFEPDIMLEG 108
Query: 127 GDEIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFL-GTYGRTDLPTGNEDKIV 185
G+ I++G++ L +IHTPGH+ GS CLY E + +F+GDTVF G++GR DLP G+ +++
Sbjct: 109 GEVIELGELSLEVIHTPGHSPGSICLYEPERKWLFSGDTVFAHGSFGRVDLPGGSARELI 168
Query: 186 ESLELLKSFDVRLGLPGHGRVIKGWKA-NLERILRVLK 222
SLE L DV PGH +I+G A ++ LR+ K
Sbjct: 169 NSLEKLSKLDVENLYPGHEDIIEGNAARHIANSLRIAK 206
>sp|Q58298|Y888_METJA HYPOTHETICAL PROTEIN MJ0888 >gi|2128038|pir||H64410 hypothetical
protein homolog MJ0888 - Methanococcus jannaschii
>gi|1591566|gb|AAB98892.1| (U67532) conserved
hypothetical protein [Methanococcus jannaschii]
Length = 206
Score = 92.8 bits (227), Expect = 3e-18
Identities = 66/201 (32%), Positives = 98/201 (47%), Gaps = 13/201 (6%)
Query: 10 ILMLEGVNLDSNVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSKVIIFNTHEH 69
IL L G SN Y + K +LI D GT +N ++ G ++++ +I NTH H
Sbjct: 2 ILKLNGYGYSSNSYLIIGKKNILI-DPGTSGTFNILMEELERNG-IKDID--LIINTHCH 57
Query: 70 FDHVGGNLVFKEKLKVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHEVELKLEDGDE 129
FDH + + +E K K L+ GD+ + S + + P E+ E +E
Sbjct: 58 FDHTSADYLIEEYFNCPTIIEDK-EVKHLKNGDEVTVSSLFGAKLNPPKEIIPLSEIEEE 116
Query: 130 IKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPTGNEDKIVESLE 189
+K L +I TPGHT GS + YE I TGDT+F GR DLPTG+ ++ S+
Sbjct: 117 LK--SYGLEIIRTPGHTYGSISIIYENSLI--TGDTIFAYGVGRWDLPTGDVIQLRNSIN 172
Query: 190 LLKSF----DVRLGLPGHGRV 206
LL+ ++ PGHG +
Sbjct: 173 LLERIANERNIDKLYPGHGEI 193
>gb|AAK43233.1| Conserved hypothetical protein [Sulfolobus solfataricus]
Length = 209
Score = 87.0 bits (212), Expect = 2e-16
Identities = 65/188 (34%), Positives = 97/188 (51%), Gaps = 11/188 (5%)
Query: 18 LDSNVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSKVIIFNTHEHFDHVGGNL 77
L +N Y + + E +I+D G + ++ + T R E ++ I TH HFDH+ G
Sbjct: 11 LATNSYLVIAGKEGVIIDAGGDM--SELIQTVRKE----KINIRYIIATHGHFDHIMGVN 64
Query: 78 VFKEKLKVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHEVELKLEDGDEIKVGKVKL 137
K F ++ L+ + S+ D + + +++GDEI++G KL
Sbjct: 65 QIKRDFPSSTFLINEKDLGLLKRASS-MAQSFLNLLISDVTKPDGFVKEGDEIELGGEKL 123
Query: 138 RLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPTGNEDKIVESLELLKSF-DV 196
++I TPGHT GS C+ +FTGDT+F GT GRTDL G+E + ESLE LK D
Sbjct: 124 KIIETPGHTMGSICII--ANGYIFTGDTLFYGTVGRTDL-GGSEKLLRESLEKLKKLPDE 180
Query: 197 RLGLPGHG 204
+ PGHG
Sbjct: 181 IIVYPGHG 188
>gi|11499983 conserved hypothetical protein [Archaeoglobus fulgidus]
>gi|7446964|pir||H69550 conserved hypothetical protein
AF2407 - Archaeoglobus fulgidus
>gi|2650685|gb|AAB91258.1| (AE001109) conserved
hypothetical protein [Archaeoglobus fulgidus]
Length = 203
Score = 86.6 bits (211), Expect = 2e-16
Identities = 56/192 (29%), Positives = 93/192 (48%), Gaps = 24/192 (12%)
Query: 19 DSNVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSKVIIFNTHEHFDHVGGNLV 78
DS+ ++ + +E+ ++D GT + RN+G E +FNTH H DH N++
Sbjct: 18 DSSNCYVIADEEVAVIDPGTFKSYTNLRGLMRNDGIGE---ANYVFNTHLHRDHCESNVL 74
Query: 79 FKEKLKVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHEVELKLEDGDEIKVGKVKLR 138
F E+ ++ F S D + Y + +++KL G +G +
Sbjct: 75 FAERGALLGFDSK-----------DMAVSQYRFN-------LDIKL--GKHFIIGNTGIE 114
Query: 139 LIHTPGHTRGSSCLYYEEERIMFTGDTVFL-GTYGRTDLPTGNEDKIVESLELLKSFDVR 197
++ TPGH+ GS Y E TGD +F G GR D+ ++ ++ S+E L+S D
Sbjct: 115 ILRTPGHSPGSLTFYLPEHSAAITGDLIFENGVPGRFDIYGSDKKELARSIEKLRSLDAE 174
Query: 198 LGLPGHGRVIKG 209
LPGHGR+++G
Sbjct: 175 YILPGHGRIMRG 186
>gi|11498145 conserved hypothetical protein [Archaeoglobus fulgidus]
>gi|7483023|pir||F69316 conserved hypothetical protein
AF0534 - Archaeoglobus fulgidus
>gi|2650086|gb|AAB90700.1| (AE001067) conserved
hypothetical protein [Archaeoglobus fulgidus]
Length = 319
Score = 83.5 bits (203), Expect = 2e-15
Identities = 64/220 (29%), Positives = 101/220 (45%), Gaps = 26/220 (11%)
Query: 20 SNVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLE--NVSKVIIFNTHEHFDHVGGNL 77
+N Y + DE L++DTG +N+ +G LE NV +F TH H DH+G
Sbjct: 24 TNSYLVMDGDEALLIDTG----FNRESCYRSIKGALEELNVRNFRVFCTHMHADHMGLAA 79
Query: 78 VFKEKLKVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHEVELKLE------------ 125
+++ + E + + +EE +L +Y + E E L
Sbjct: 80 KLADEVLMGETEAKIVRETMVEEDYWRDLLKFYIKNGFPAKEAEKILSLHPGRNFWVKDD 139
Query: 126 -----DGDEIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPTGN 180
G+ ++VG +L+ I TPGH+ G +CLY E +I F+GD V +
Sbjct: 140 VEFVAVGEVVRVGSFRLKAIETPGHSPGHTCLYDESRKIFFSGDHVLIDITPNITWWPFL 199
Query: 181 EDKI---VESLELLKSFDVRLGLPGHGRVIKGWKANLERI 217
ED + ++SLE +++ DV+L LPGH R K +E I
Sbjct: 200 EDSLSSYLQSLEKVENLDVKLVLPGHRRRWNDLKTRVEEI 239
>sp|O67893|YL35_AQUAE HYPOTHETICAL PROTEIN AQ_2135 >gi|7446954|pir||A70483 conserved
hypothetical protein aq_2135 - Aquifex aeolicus
>gi|2984341|gb|AAC07862.1| (AE000774) hypothetical
protein [Aquifex aeolicus]
Length = 211
Score = 83.5 bits (203), Expect = 2e-15
Identities = 62/187 (33%), Positives = 101/187 (53%), Gaps = 15/187 (8%)
Query: 4 IIIKPNILMLEGVNLDSNVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSKVI- 62
+IIK ++ + + ++ +V + E +I+D G K L+ ++ KV+
Sbjct: 1 MIIK--VIPVGPIAVNCSVVADEKSGEAIIIDPGAEA--EKILEAVKD-------FKVVG 49
Query: 63 IFNTHEHFDHVGGNLVFKEKLKVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHEVEL 122
I TH H DHVG KE L V F + L K L + + + Y G P E ++
Sbjct: 50 IVATHGHIDHVGQVGKLKE-LFDVPFYMNPLD-KFLINDEIWSGFASYIGAVPCP-EPDV 106
Query: 123 KLEDGDEIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPTGNED 182
++ +GD I+VG V+ +++HTPGHT G CLY E+ R++ GD +F G+ GR DLP GN
Sbjct: 107 EISEGDVIRVGDVEFKVLHTPGHTPGLCCLYEEKRRVLIAGDLLFKGSVGRWDLPGGNLV 166
Query: 183 KIVESLE 189
++ +S++
Sbjct: 167 ELKKSVK 173
>sp|P54501|YQGX_BACSU HYPOTHETICAL 23.2 KDA PROTEIN IN SODA-COMGA INTERGENIC REGION
>gi|7446952|pir||A69958 conserved hypothetical protein
yqgX - Bacillus subtilis >gi|1303871|dbj|BAA12527.1|
(D84432) YqgX [Bacillus subtilis]
>gi|2634913|emb|CAB14410.1| (Z99116) similar to
hypothetical proteins [Bacillus subtilis]
Length = 211
Score = 77.6 bits (188), Expect = 1e-13
Identities = 57/193 (29%), Positives = 90/193 (46%), Gaps = 18/193 (9%)
Query: 18 LDSNVYFLKSKDELLIVDTG-TGVYWNKYLDTARNEGWLENVSKVIIFNTHEHFDHVGGN 76
+ +N YFL S D+ LI D G G N+Y+ + ++ + I TH HFDH+G
Sbjct: 11 IQANAYFLISDDQCLIFDPGGEGHKINQYIKE-------KGLTPLAILLTHAHFDHIGAL 63
Query: 77 LVFKEKLKVVEFASHK---LTAKALEEGDDYIILSYYYGRRYDPHEVELKLEDGDEIKVG 133
+EK + + A A G + G + +E E+ +G
Sbjct: 64 DEVREKWDIPVYLHQNEKNWLADASLNGSGML-----RGIEVTAKPADHLIEGDGELNIG 118
Query: 134 KVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPTGNEDKIVESL--ELL 191
L + TPGH+ GS Y ++ ++ +GD +F G GRTDL GN++ ++ S+ +LL
Sbjct: 119 PFHLETLFTPGHSPGSVSYYVKDADLVISGDVLFQGGIGRTDLIGGNQETLLTSIHEKLL 178
Query: 192 KSFDVRLGLPGHG 204
+ L L GHG
Sbjct: 179 TLPEHTLVLSGHG 191
>pir||F82220 glyoxylase II family protein VC1270 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9655755|gb|AAF94429.1|
(AE004206) glyoxylase II family protein [Vibrio
cholerae]
Length = 218
Score = 76.5 bits (185), Expect = 3e-13
Identities = 52/145 (35%), Positives = 73/145 (49%), Gaps = 11/145 (7%)
Query: 66 THEHFDHVGGNLVFKEKLKVVEFAS-HKLTAKALE--EGDDYIILSYYYGRRYDPHEVEL 122
TH H DHVGG + L HK L+ EG + + ++P E
Sbjct: 57 THGHLDHVGGTQSLAQALGGTRVVGPHKADNFWLQGLEGQSKMF-GFPLTEAFEPDEW-- 113
Query: 123 KLEDGDEIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPTGNED 182
L+DGD I G+ L++IHTPGHT G L+ E R+ F GD +F G+ GRTD P G+ +
Sbjct: 114 -LDDGDVIHFGQQTLQVIHTPGHTPGHVVLFSEAARLAFVGDVLFNGSIGRTDFPQGDFN 172
Query: 183 KIVESLE---LLKSFDVRLGLPGHG 204
++ S++ DV +PGHG
Sbjct: 173 TLIASIKNKLWPLGSDVTF-IPGHG 196
>pir||D72599 hypothetical protein APE1260 - Aeropyrum pernix (strain K1)
>gi|5104936|dbj|BAA80250.1| (AP000061) 209aa long
hypothetical protein [Aeropyrum pernix]
Length = 209
Score = 76.1 bits (184), Expect = 3e-13
Identities = 58/190 (30%), Positives = 90/190 (46%), Gaps = 15/190 (7%)
Query: 18 LDSNVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSKVIIFNTHEHFDHVGGNL 77
L N Y + E L+VD G + L+ G + +++ TH HFDHV G
Sbjct: 12 LQVNTYVAAAGGECLVVDPGGP----EVLEALERLGCRD----IVVALTHGHFDHVAGVE 63
Query: 78 VFKEKLKVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHEVELKLEDGDEIKVGKVKL 137
+ VV A+H T + E D + Y + V+++L DG ++ + L
Sbjct: 64 HLAARGAVV--AAHPQTPRVAEAYADLGVKMGYL-KGVPRISVDVELVDGSTLRAAGLSL 120
Query: 138 RLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPTGNEDKIVESL-ELLKSF-- 194
+ IHTPGH+ LY + + FTGD +F G+ GR D+ G+ + + SL +++
Sbjct: 121 KAIHTPGHSPDHIVLYDQARGLAFTGDLIFRGSIGRVDIALGDGEAMARSLARIVRELPR 180
Query: 195 DVRLGLPGHG 204
RL LPGHG
Sbjct: 181 GTRL-LPGHG 189
>emb|CAC11806.1| (AL445065) conserved hypothetical protein [Thermoplasma
acidophilum]
Length = 322
Score = 75.3 bits (182), Expect = 6e-13
Identities = 62/227 (27%), Positives = 102/227 (44%), Gaps = 42/227 (18%)
Query: 20 SNVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSKV-IIFNTHEHFDHVGGNLV 78
+N+Y L++ D ++D+G K ++ +N+ K+ +F TH H DH+GG L
Sbjct: 30 ANIYRLETDDSAFLIDSGMSDNSIKSIE--------QNLKKIDFVFITHLHIDHLGGVLY 81
Query: 79 FKEKLKVVEFASH---KLTAKALEEGDDYI-------------------ILSYYYGRRYD 116
EK + + +H L A E+ D YI I++ + RY
Sbjct: 82 LHEKYGIPAYMNHLDLDLIMNANEDRDGYIKRHSEIFRVNGVPPSMVEEIITMHPVIRYF 141
Query: 117 PHEVELKLEDGDEIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVF------LGT 170
+ +L D K+ ++ + PGH+ GS+ +Y E+ R +F GD + +
Sbjct: 142 DYYSKLDFVQ-DYRKINASGIKFVEVPGHSPGSTAVYIEDSRDLFAGDHILDRITPNIAV 200
Query: 171 YGRTDLPTGNEDKIVESLELLKSFDVRLGLPGHGRVIKGWKANLERI 217
YG D GN +ESLE +++ V PGHG VI K ++ I
Sbjct: 201 YGDED-DLGN---YLESLEKVRAMKVDRIFPGHGNVITEPKIRIDDI 243
>dbj|BAB06539.1| (AP001516) unknown conserved protein [Bacillus halodurans]
Length = 211
Score = 74.9 bits (181), Expect = 8e-13
Identities = 46/174 (26%), Positives = 86/174 (48%), Gaps = 5/174 (2%)
Query: 35 DTGTGVYWNKYLDTARNEGWLEN--VSKVIIFNTHEHFDHVGGNLVFKEKLKVVEFASHK 92
D G G+ ++ + + WL + ++ + I TH HFDH+G + + + H+
Sbjct: 21 DQGEGIIFDPGGEVEKLITWLRDRQITPLAILLTHAHFDHIGAVEDVRNTFHIPVYI-HE 79
Query: 93 LTAKALEEGDDYIILSYYYGRRYDPHEVELKLEDGDEIKVGKVKLRLIHTPGHTRGSSCL 152
+ L + + G E E + ++ +G +++ TPGH+ GS
Sbjct: 80 NEKEWLIDPQRNGSSLFIPGSSIKAREAEHLITGEQDLSIGSFSYQVLETPGHSPGSLSY 139
Query: 153 YYEEERIMFTGDTVFLGTYGRTDLPTGNEDKIVESL--ELLKSFDVRLGLPGHG 204
Y +E++I+F+GD +F G+ GRTDLP G+ +++S+ +LL+ + GHG
Sbjct: 140 YAKEDKIVFSGDALFAGSIGRTDLPGGDHQLLLDSIHDKLLELPEDTTVASGHG 193
>gb|AAK42646.1| Conserved hypothetical protein [Sulfolobus solfataricus]
Length = 310
Score = 74.9 bits (181), Expect = 8e-13
Identities = 60/224 (26%), Positives = 106/224 (46%), Gaps = 37/224 (16%)
Query: 21 NVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSKV-----IIFNTHEHFDHVGG 75
N Y +K +E +++DTG L T + L N K + TH H DH+G
Sbjct: 18 NSYLVKGNEESVLIDTG--------LPTQEDVTTLSNYLKAYGYPNFVVITHYHPDHMGL 69
Query: 76 NLVFKEKLKVV------EFASHKLTAKALEEGDDYIILSYY--------YGRRYDPHEVE 121
+FK+K ++ E+ ++ L+ + +E Y++ + + + R E+
Sbjct: 70 VRLFKDKSIILISDRELEYVTYLLSEEYEKEMRKYLLANGFPEEFIQRMFRNRSRFSEII 129
Query: 122 -----LKLEDGDEIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDL 176
++DGD IK+G+ ++R++ TPGHT G CL Y E+++F GD + L
Sbjct: 130 DGVNFNTVKDGDVIKLGQEQMRVLLTPGHTMGHICLVY--EKVVFCGDHILQDITPNISL 187
Query: 177 PTGNEDKI---VESLELLKSFDVRLGLPGHGRVIKGWKANLERI 217
++ + +ESL+ ++ D+ L P HG V+K +E I
Sbjct: 188 LRLEDNPLKAYLESLDKVQKLDIELLYPAHGEVLKDVSKRVEEI 231
>sp|P75849|YCBL_ECOLI HYPOTHETICAL 23.8 KDA PROTEIN IN MUKB-ASPC INTERGENIC REGION
>gi|7446946|pir||F64832 probable hydrolase (EC 3.-.-.-)
ycbL - Escherichia coli >gi|1787158|gb|AAC74013.1|
(AE000195) orf, hypothetical protein [Escherichia coli
K12] >gi|4062494|dbj|BAA35673.1| (D90730) Hypothetical
23.2 kd protein in sodA-comGA intergenic region.
[Escherichia coli] >gi|4062497|dbj|BAA35679.1| (D90731)
Hypothetical 23.2 kd protein in sodA-comGA intergenic
region. [Escherichia coli]
Length = 215
Score = 74.1 bits (179), Expect = 1e-12
Identities = 45/145 (31%), Positives = 71/145 (48%), Gaps = 6/145 (4%)
Query: 63 IFNTHEHFDHVGGNLVFKEKLKVVEFASHKLTAKALEEGDDYIILSYYYG-RRYDPHEVE 121
I TH H DHVG + V F K L+ S +G P +
Sbjct: 52 ILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQ---GLPAQSRMFGLEECQPLTPD 108
Query: 122 LKLEDGDEIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPTGNE 181
L +GD I +G V L+++H PGHT G + + +++ +GD +F G GR+D P G+
Sbjct: 109 RWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGRSDFPRGDH 168
Query: 182 DKIVESL--ELLKSFDVRLGLPGHG 204
++++ S+ +LL D + +PGHG
Sbjct: 169 NQLISSIKDKLLPLGDDVIFIPGHG 193
>pir||B81011 glyoxalase II family protein NMB2049 [imported] - Neisseria
meningitidis (group B strain MD58)
>gi|7227311|gb|AAF42369.1| (AE002555) glyoxalase II
family protein [Neisseria meningitidis MC58]
Length = 210
Score = 73.4 bits (177), Expect = 2e-12
Identities = 47/152 (30%), Positives = 74/152 (47%), Gaps = 16/152 (10%)
Query: 63 IFNTHEHFDHVGGNLVFKEKLKVVEFASHKLTAKALEEGDDYIILSY-----YYGRRYDP 117
I+ TH H DH GG + E LK +HK+ D++++ S YG P
Sbjct: 54 IWLTHGHLDHAGGVV---EMLK-----THKVPVLGPHPDDEFLLQSLPQTTAQYGFPVSP 105
Query: 118 HEVELK-LEDGDEIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDL 176
+ LE+G+ + VG+ +++H PGHT G Y E ++ GD +F T GRTD
Sbjct: 106 AFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETIGRTDF 165
Query: 177 PTGNEDKIVESL--ELLKSFDVRLGLPGHGRV 206
P GN ++ ++ +L + + GHGR+
Sbjct: 166 PRGNHADLINNIRNKLFTLPETVQVVAGHGRM 197
>pir||A81955 probable hydrolase NMA0387 [imported] - Neisseria meningitidis
(group A strain Z2491) >gi|7379140|emb|CAB83688.1|
(AL162753) putative hydrolase [Neisseria meningitidis
Z2491]
Length = 210
Score = 73.4 bits (177), Expect = 2e-12
Identities = 47/152 (30%), Positives = 74/152 (47%), Gaps = 16/152 (10%)
Query: 63 IFNTHEHFDHVGGNLVFKEKLKVVEFASHKLTAKALEEGDDYIILSY-----YYGRRYDP 117
I+ TH H DH GG + E LK +HK+ D++++ S YG P
Sbjct: 54 IWLTHGHLDHAGGVV---EMLK-----THKVPVLGPHPDDEFLLQSLPQTTAQYGFPVSP 105
Query: 118 HEVELK-LEDGDEIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDL 176
+ LE+G+ + VG+ +++H PGHT G Y E ++ GD +F T GRTD
Sbjct: 106 AFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETIGRTDF 165
Query: 177 PTGNEDKIVESL--ELLKSFDVRLGLPGHGRV 206
P GN ++ ++ +L + + GHGR+
Sbjct: 166 PRGNHADLINNIRNKLFALPETVQVVAGHGRM 197
>emb|CAC14523.1| (AL446004) possible CGI-83 protein [Leishmania major]
Length = 336
Score = 73.4 bits (177), Expect = 2e-12
Identities = 63/221 (28%), Positives = 100/221 (44%), Gaps = 37/221 (16%)
Query: 6 IKPNILMLEGVNLDSNVYFLKSKDELLIVDTGTGVYWNKYL------DTARNEGWLENVS 59
+ P + L+G SN Y + + E L++D+G GV +L + + G ++S
Sbjct: 27 LNPGYMTLQG----SNTYLVGTGQERLLIDSGEGVEGYGHLLQKAVAEESTRLGGPVHIS 82
Query: 60 KVIIFNTHEHFDHVGGNLVFKEKLKVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHE 119
K+++ TH H DH+GG + V+ +Y E
Sbjct: 83 KLLL--THWHGDHIGGVETVRRLFPQVQLLKQP--------------------SQYAHTE 120
Query: 120 VELKLEDGDE-IKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPT 178
V+ + E +KV L+L+HTPGHT C + +EER +FT DTV LGT T + +
Sbjct: 121 VDALCQVPPEVVKVEGATLQLVHTPGHTDDHLCAFLQEERALFTSDTV-LGT--GTSVFS 177
Query: 179 GNEDKIVESLELLKSFDVRLGLPGHGRVIKGWKANLERILR 219
D + SL +L+ P HG V++ A +E I+R
Sbjct: 178 SFRD-YMASLHVLERMKPERLYPAHGPVVEDGTARIEEIIR 217
>gb|AAG10453.1|AF279106_15 (AF279106) predicted metallobeta lactamase fold protein [uncultured
proteobacterium EBAC31A08]
Length = 265
Score = 72.6 bits (175), Expect = 4e-12
Identities = 55/196 (28%), Positives = 87/196 (44%), Gaps = 20/196 (10%)
Query: 24 FLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSKVIIFNTHEHFDHVGGNLVFKEKL 83
+L KD++ +VD G + +++LD G + IF TH H DH L + L
Sbjct: 23 YLIGKDDITLVDPGPNI--SEHLDEIIKAG---DGKIKRIFVTHTHTDHSPAALPLSKTL 77
Query: 84 KVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHEVELKLEDGDEIKVGKVKLRLIHTP 143
V + L +G+ S + + P ++ L D D I+ + L +IHTP
Sbjct: 78 NVPMYGR-------LVDGE-----SSWEDETFIP---DIILNDKDIIETDEYTLEVIHTP 122
Query: 144 GHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPTGNEDKIVESLELLKSFDVRLGLPGH 203
GH C ++ + + TGD + G+ P GN + SLE L FD+ PGH
Sbjct: 123 GHASNHLCFLIKDTKCLLTGDHIMDGSTVVIGPPDGNMTSYINSLEKLLDFDIDCFAPGH 182
Query: 204 GRVIKGWKANLERILR 219
G I + ++ I+R
Sbjct: 183 GNYIHEPEKTIQSIIR 198
>pir||A81353 probable hydrolase Cj0809c [imported] - Campylobacter jejuni
(strain NCTC 11168) >gi|6968255|emb|CAB73074.1|
(AL139076) putative hydrolase [Campylobacter jejuni]
Length = 198
Score = 71.0 bits (171), Expect = 1e-11
Identities = 50/191 (26%), Positives = 93/191 (48%), Gaps = 26/191 (13%)
Query: 19 DSNVYFLKSKDELLIVDTGTG--VYWNKYLDTARNEGWLENVSKVIIFNTHEHFDHVGGN 76
++N Y L + I+D GT ++ K++ + + I NTH H+DH+ N
Sbjct: 12 ETNCYILFTPKGEFIIDPGTDALLFIKKHVK-----------NPLAILNTHGHYDHIWDN 60
Query: 77 LVFKEKLKVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHEVELKLEDGDEIKVGKVK 136
K++ + + HK +D +L + + YD + + +++ D + + V
Sbjct: 61 AKVKKEYNIPIYI-HK---------NDAFMLHDDFNQGYDSSDADFLVDNEDILNICGVD 110
Query: 137 LRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPTGNEDKIVESLELLKSF-- 194
+ PGHT G + + + IMF+GD +F + GR D P + + + +SLE + ++
Sbjct: 111 FKFHFLPGHTPGCTMIEIVGKNIMFSGDFLFYRSIGRWDFPYSDANLMKQSLEKVMTYKE 170
Query: 195 DVRLGLPGHGR 205
D +L LPGHG+
Sbjct: 171 DFKL-LPGHGQ 180
>gb|AAG19667.1| (AE005054) flavoprotein; FprA [Halobacterium sp. NRC-1]
Length = 192
Score = 69.1 bits (166), Expect = 4e-11
Identities = 56/195 (28%), Positives = 86/195 (43%), Gaps = 36/195 (18%)
Query: 15 GVNLDSNVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSKVIIFNTHEHFDHVG 74
GV ++ FL + D ++VD G + R+ G V+ + TH H DHVG
Sbjct: 8 GVQAFTSNVFLVTGDRDVVVDAGANY---DVVAAVRDHG----VTPDAVVITHPHPDHVG 60
Query: 75 GNLVFKEKLKVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHEVELKLEDGDEIKVGK 134
AL + D +L + + + L DGDEI++G
Sbjct: 61 N-------------------LDALVDAFDPTVLGFD-----GVADADRALADGDEIRLGD 96
Query: 135 VKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFL-GTYGRTDLPTGNEDKIVESLELLKS 193
++HTPGH CLY ++F+ D VF G++GRTDL G+ + +VES++ L
Sbjct: 97 DDYVVLHTPGHEPHHVCLYSPAAGVLFSADLVFANGSFGRTDLDGGDRETLVESIDRLAD 156
Query: 194 F----DVRLGLPGHG 204
++ PGHG
Sbjct: 157 TVAGEELTALYPGHG 171
>sp|Q49649|YP81_MYCLE HYPOTHETICAL 23.9 KDA PROTEIN MLCB1259.11/B1177_C3_247
>gi|2145639|pir||S72738 B1177_C3_247 protein -
Mycobacterium leprae >gi|466827|gb|AAA17102.1| (U00011)
B1177_C3_247 [Mycobacterium leprae]
>gi|3136014|emb|CAA19086.1| (AL023591) hypothetical
protein MLCB1259.11 [Mycobacterium leprae]
Length = 218
Score = 67.9 bits (163), Expect = 1e-10
Identities = 38/92 (41%), Positives = 55/92 (59%), Gaps = 3/92 (3%)
Query: 116 DPHEVELKLEDGDEIKVGKVKLRLIHTPGHTRGSSCLYYEEER-IMFTGDTVFLGTYGRT 174
+P +V DGD++ +G V + + HTPGHTRGS C + + ++ TGDT+F T GRT
Sbjct: 109 EPKQVVELDRDGDKLDLGSVTVNVDHTPGHTRGSVCFWVAADTDVVLTGDTLFERTIGRT 168
Query: 175 DLPTGNEDKIVESL--ELLKSFDVRLGLPGHG 204
DL G+ + S+ +LL D + LPGHG
Sbjct: 169 DLFGGSGRDLYRSIVEKLLVLDDKTVVLPGHG 200
>sp|Q57544|YCBL_HAEIN HYPOTHETICAL PROTEIN HI1663 >gi|1074886|pir||D64174 probable
hydrolase (EC 3.-.-.-) HI1663 - Haemophilus influenzae
(strain Rd KW20) >gi|1574513|gb|AAC23309.1| (U32839)
conserved hypothetical protein [Haemophilus influenzae
Rd]
Length = 212
Score = 67.9 bits (163), Expect = 1e-10
Identities = 44/152 (28%), Positives = 73/152 (47%), Gaps = 17/152 (11%)
Query: 62 IIFNTHEHFDHVGGNLVFKEKLKVVEFASHKLTAKALEEGDDYIILSY-YYGRRYDPHEV 120
++ TH H DHVG + K+ V + S+ E D ++ S +R+ +
Sbjct: 50 VLLLTHGHLDHVGAAMQLKQHFGVEIWGSN--------EKDKFLFESLPEQAQRFGLPNI 101
Query: 121 ELKLED------GDEIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRT 174
+ L D G+ +K+ ++H PGHT G E+++ FTGD +F G GRT
Sbjct: 102 DAFLPDRWFNQEGEILKLDGFNFEILHLPGHTPGHIGFIEHEKKVAFTGDVLFQGGIGRT 161
Query: 175 DLPTGNEDKIVESL--ELLKSFDVRLGLPGHG 204
D P G+ + ++ S+ +LL D + + GHG
Sbjct: 162 DFPRGDYETLISSIRTKLLPLNDDIIIIAGHG 193
>pir||A75189 hypothetical protein PAB0023 - Pyrococcus abyssi (strain Orsay)
>gi|5457469|emb|CAB48960.1| (AJ248283) hypothetical
protein [Pyrococcus abyssi]
Length = 204
Score = 67.9 bits (163), Expect = 1e-10
Identities = 56/196 (28%), Positives = 88/196 (44%), Gaps = 34/196 (17%)
Query: 21 NVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSKVIIFNTHEHFDHVGGNLVFK 80
NVY + D L++VDTG K ++ + G + + VI+ TH H DH G K
Sbjct: 20 NVYIVDRGDHLVLVDTGIESTCEKIIEAVKKLG--KPLKAVIL--THGHMDHTGSLRCLK 75
Query: 81 EKLKVVEFASHKLTAKALEEGDDYIILSYYYGRRYDPHEVELKLEDGDEIKVGKVKLRLI 140
E L + AS + + +E +V++KL+DG+ + R++
Sbjct: 76 EVLNPI-VASQEEEVEMIERNTGV--------------KVDVKLKDGELFE----GFRVL 116
Query: 141 HTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDL-------PTGNEDKIVESLELLKS 193
H PGHT+GS CL E+ + +F GD V ++ P N +I+E LE+
Sbjct: 117 HKPGHTKGSICLLDEDSKSLFVGDLVVEENGKLKEVPHQYSMDPEMNRKRIIELLEV--- 173
Query: 194 FDVRLGLPGHGRVIKG 209
+ +P HG I G
Sbjct: 174 -EFENLMPAHGNPIIG 188
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.320 0.142 0.421
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 91620533
Number of Sequences: 2977
Number of extensions: 4151565
Number of successful extensions: 9183
Number of sequences better than 1.0e-10: 26
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 22
Number of HSP's that attempted gapping in prelim test: 9138
Number of HSP's gapped (non-prelim): 27
length of query: 222
length of database: 189,106,746
effective HSP length: 52
effective length of query: 170
effective length of database: 157,985,422
effective search space: 26857521740
effective search space used: 26857521740
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 163 (67.9 bits)