BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0704 (PAB0704) DE:THERMONUCLEASE PRECURSOR (EC 3.1.31.1)
(175 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||D75083 micrococcal nuclease (EC 3.1.31.1) PAB0704 precursor... 354 3e-97
pir||F71064 micrococcal nuclease (EC 3.1.31.1) PH1212 precursor ... 294 4e-79
sp|Q58834|YE39_METJA HYPOTHETICAL PROTEIN MJ1439 >gi|2129300|pir... 110 1e-23
gb|AAG20014.1| (AE005082) Vng1794c [Halobacterium sp. NRC-1] 89 4e-17
gi|9507655 100% identical to gp:ECR100TRA_9[orfA, putative ORF, ... 81 1e-14
gi|10955331 hypothetical 15.6 kD protein in fino 3'region precur... 81 1e-14
pdb|1SYB| Staphylococcal Nuclease (E.C.3.1.31.1) With Residues... 80 2e-14
sp|P43270|NUC_STAHY THERMONUCLEASE PRECURSOR (TNASE) (MICROCOCCA... 78 5e-14
gi|9630131 putative DNase/RNase endonuclease [Bacteriophage SPBc... 76 2e-13
gi|10956387 pXO1-141 [Bacillus anthracis] >gi|7474251|pir||D5910... 74 9e-13
pdb|1SNQ| Protein Stability In Staphylococcal Nuclease 74 9e-13
pdb|1NSN|S Chain S, Immunoglobulin, Staphylococcal Nuclease Mol_... 74 1e-12
pdb|1SYG| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant With P... 74 1e-12
pdb|1EY8|A Chain A, Structure Of S. Nuclease Stabilizing Triple ... 74 1e-12
pdb|1SYF| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant With P... 74 1e-12
pdb|1SYD| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant With P... 73 2e-12
pir||D69888 micrococcal nuclease homolog yncB - Bacillus subtili... 73 2e-12
pdb|1SNP| Protein Stability In Staphylococcal Nuclease 73 2e-12
pir||H81372 probable micrococcal nuclease (EC 3.1.31.1) Cj0979c ... 73 2e-12
pdb|1EY7|A Chain A, Structure Of S. Nuclease Stabilizing Mutant ... 73 2e-12
pdb|1EY9|A Chain A, Structure Of S. Nuclease Stabilizing Quadrup... 73 2e-12
sp|P43269|NUC_STAIN THERMONUCLEASE PRECURSOR (TNASE) (MICROCOCCA... 73 2e-12
sp|P00644|NUC_STAAU THERMONUCLEASE PRECURSOR (TNASE) (MICROCOCCA... 73 2e-12
prf||1109959A nuclease,staphylococcal [Staphylococcus aureus] 73 2e-12
pdb|1EY0|A Chain A, Structure Of Wild-Type S. Nuclease At 1.6 A ... 73 2e-12
pdb|1STY| Staphylococcal Nuclease (E.C.3.1.31.1) Insertion Mut... 73 2e-12
pdb|1SNO| Protein Stability In Staphylococcal Nuclease 73 2e-12
gb|AAC14660.1| (AF038666) deltaSP-Nuc [Cloning vector pFUN] 73 2e-12
pdb|1EYA|A Chain A, Structure Of S. Nuclease Stabilizing Quintup... 73 2e-12
pdb|1EYC|A Chain A, Structure Of S. Nuclease Stabilizing Quintup... 73 2e-12
pdb|1EZ6|A Chain A, Structure Of S. Nuclease Stabilizing Sextupl... 73 3e-12
pdb|2SNM| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant With V... 73 3e-12
pdb|1KDB| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant With L... 73 3e-12
pdb|1EY4|A Chain A, Structure Of S. Nuclease Stabilizing Mutant ... 73 3e-12
pdb|1EY5|A Chain A, Structure Of S. Nuclease Stabilizing Mutant ... 73 3e-12
pdb|1EY6|A Chain A, Structure Of S. Nuclease Stabilizing Mutant ... 73 3e-12
pdb|1STA| Staphylococcal Nuclease (E.C.3.1.31.1) Double Insert... 72 4e-12
pdb|1SNM| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant (Glu 4... 72 5e-12
pdb|2SNS| Staphylococcal Nuclease (E.C.3.1.33.1) Complex With ... 72 5e-12
pdb|1KDC| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant With L... 72 5e-12
pdb|1SND|A Chain A, Staphylococcal Nuclease Dimer Containing A D... 72 5e-12
pdb|1ENC| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant With A... 71 6e-12
pdb|1KDA| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant With L... 71 6e-12
pdb|3NUC| Staphlococcal Nuclease, 1-N-Propane Thiol Disulfide ... 71 8e-12
pdb|1NUC| Staphylococcal Nuclease, V23c Variant 71 8e-12
pdb|2ENB| Staphylococcal Nuclease (E.C.3.1.31.1) Mutation With... 70 1e-11
pdb|1KAA| Staphylococcal Nuclease (E.C.3.1.33.1) Mutant With L... 69 2e-11
pdb|1KAB| Staphylococcal Nuclease (E.C.3.1.33.1) Mutant With L... 69 3e-11
pdb|1STB| Staphylococcal Nuclease (E.C.3.1.31.1) Insertion Mut... 68 5e-11
gi|10957342 putative partition protein [Salmonella typhi] >gi|78... 68 5e-11
sp|Q57519|YC96_HAEIN HYPOTHETICAL PROTEIN HI1296 PRECURSOR >gi|1... 68 9e-11
>pir||D75083 micrococcal nuclease (EC 3.1.31.1) PAB0704 precursor - Pyrococcus
abyssi (strain Orsay) >gi|5458481|emb|CAB49969.1|
(AJ248286) THERMONUCLEASE PRECURSOR (EC 3.1.31.1)
[Pyrococcus abyssi]
Length = 175
Score = 354 bits (900), Expect = 3e-97
Identities = 175/175 (100%), Positives = 175/175 (100%)
Query: 1 MARLFGRAGRFVSSIILIALILGCLSQPKELHGKVVKVVDGDTVYVKLSNGETVKVRLIG 60
MARLFGRAGRFVSSIILIALILGCLSQPKELHGKVVKVVDGDTVYVKLSNGETVKVRLIG
Sbjct: 1 MARLFGRAGRFVSSIILIALILGCLSQPKELHGKVVKVVDGDTVYVKLSNGETVKVRLIG 60
Query: 61 IDAPELEEDLMRPGEYPGIHNLSCLVKYGYIAKEFLKNYTLGKDVTLIFDSIQGRKDKYG 120
IDAPELEEDLMRPGEYPGIHNLSCLVKYGYIAKEFLKNYTLGKDVTLIFDSIQGRKDKYG
Sbjct: 61 IDAPELEEDLMRPGEYPGIHNLSCLVKYGYIAKEFLKNYTLGKDVTLIFDSIQGRKDKYG 120
Query: 121 RLLAYLYINGTDVNAELVKMGLARVFYEKKFDKIKEYSELEDEARKRRQNLWSCN 175
RLLAYLYINGTDVNAELVKMGLARVFYEKKFDKIKEYSELEDEARKRRQNLWSCN
Sbjct: 121 RLLAYLYINGTDVNAELVKMGLARVFYEKKFDKIKEYSELEDEARKRRQNLWSCN 175
>pir||F71064 micrococcal nuclease (EC 3.1.31.1) PH1212 precursor - Pyrococcus
horikoshii >gi|3257629|dbj|BAA30312.1| (AP000005) 176aa
long hypothetical thermonuclease [Pyrococcus horikoshii]
Length = 176
Score = 294 bits (745), Expect = 4e-79
Identities = 138/176 (78%), Positives = 159/176 (89%), Gaps = 1/176 (0%)
Query: 1 MARLFGRAGRFVSSIILIALI-LGCLSQPKELHGKVVKVVDGDTVYVKLSNGETVKVRLI 59
M LFGRAGR SS++LI L+ LGC+SQ KELHGKV+KVVDGDT+YVKL +GE VKVRL+
Sbjct: 1 MVELFGRAGRLGSSLLLILLLSLGCISQSKELHGKVIKVVDGDTLYVKLDDGEVVKVRLV 60
Query: 60 GIDAPELEEDLMRPGEYPGIHNLSCLVKYGYIAKEFLKNYTLGKDVTLIFDSIQGRKDKY 119
G+DAPELEE LMRPGEYPGIHN++CL+KYG +AKEFLKN TLGK VTL FDSIQGRKDKY
Sbjct: 61 GVDAPELEEGLMRPGEYPGIHNMTCLIKYGELAKEFLKNITLGKSVTLEFDSIQGRKDKY 120
Query: 120 GRLLAYLYINGTDVNAELVKMGLARVFYEKKFDKIKEYSELEDEARKRRQNLWSCN 175
GRLL Y+Y+NGTD+NAEL+KMG ARVFYEK+F+KIKEYS+LED+ARKRR NLWSCN
Sbjct: 121 GRLLVYMYLNGTDINAELIKMGFARVFYEKRFEKIKEYSKLEDDARKRRLNLWSCN 176
>sp|Q58834|YE39_METJA HYPOTHETICAL PROTEIN MJ1439 >gi|2129300|pir||F64479 micrococcal
nuclease (EC 3.1.31.1) MJ1439 precursor - Methanococcus
jannaschii >gi|1592087|gb|AAB99448.1| (U67584)
thermonuclease [Methanococcus jannaschii]
Length = 207
Score = 110 bits (272), Expect = 1e-23
Identities = 66/151 (43%), Positives = 93/151 (60%), Gaps = 12/151 (7%)
Query: 32 HGKVVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEY-----PGIHNLSCLV 86
+GKVVKVVDGDTVYV++ NGE K+RL+G+D PE+ + P EY I + L
Sbjct: 56 YGKVVKVVDGDTVYVEV-NGELWKIRLLGVDTPEIHKR-NNPYEYYLLNGTPITDTKYLK 113
Query: 87 KYGYIAKEFLKNYTLGKDVTLIFDSIQGRKDKYGRLLAYLYINGT----DVNAELVKMGL 142
++GY AK F + K V ++FD+ +KDKYGR LAY++IN + + N EL+K G
Sbjct: 114 EWGYKAKHFAEKELKNKTVIIVFDNEAPKKDKYGRYLAYIFINNSNNLINFNEELLKYGY 173
Query: 143 ARVFYEKKFDKIKEYSELEDEARKRRQNLWS 173
ARV Y F+ E+ +E EA++ R LW+
Sbjct: 174 ARV-YISNFELKDEFLNVEREAKENRVGLWN 203
>gb|AAG20014.1| (AE005082) Vng1794c [Halobacterium sp. NRC-1]
Length = 694
Score = 88.5 bits (216), Expect = 4e-17
Identities = 55/139 (39%), Positives = 74/139 (52%), Gaps = 2/139 (1%)
Query: 35 VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCLVKYGYIAKE 94
VV+V DGDTV V+ +G T VR++GID PE + R EY GI + + L G A
Sbjct: 302 VVRVTDGDTVDVRFRDGTTDTVRIVGIDTPETGQTTERVAEYEGITDAAALKARGDAATA 361
Query: 95 FLKNYTLGKDVTLIFDSIQGRKDKYGRLLAYLYI-NGTDVNAELVKMGLARVFYEKKFDK 153
+ ++ G+ VTL FD+ + YGRLL YL + +GT N V G+ARV Y F +
Sbjct: 362 YARDALGGQTVTLSFDAGGPARGDYGRLLGYLTLPDGTTYNRRAVADGMARV-YASAFSQ 420
Query: 154 IKEYSELEDEARKRRQNLW 172
+Y E AR LW
Sbjct: 421 YDDYWAAEATARAAGDGLW 439
>gi|9507655 100% identical to gp:ECR100TRA_9[orfA, putative ORF, gene product
of plasmid R100] [Plasmid R100]
>gi|1175987|sp|Q99342|YFI3_ECOLI HYPOTHETICAL 15.6 KD
PROTEIN IN FINO 3'REGION PRECURSOR (ORFA)
>gi|96073|pir||S10665 hypothetical protein A (finO 3'
region) - Escherichia coli plasmid R100
>gi|42629|emb|CAA39343.1| (X55815) orfA [Escherichia
coli] >gi|5103254|dbj|BAA78890.1| (AP000342) 100%
identical to gp:ECR100TRA_9[orfA, putative ORF, gene
product of plasmid R100] [Plasmid R100]
Length = 153
Score = 80.8 bits (196), Expect = 1e-14
Identities = 52/160 (32%), Positives = 91/160 (56%), Gaps = 29/160 (18%)
Query: 18 IALILGCLSQP---KELHGKVVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPG 74
I L+L S P ++HG+VV+V+DGDT+ V + + + V++RL+ IDAPE ++D
Sbjct: 5 IPLVLFIFSWPVLCADIHGRVVRVLDGDTIEV-MDSRKAVRIRLVNIDAPEKKQD----- 58
Query: 75 EYPGIHNLSCLVKYGYIAKEFLKNYTLGKDVTLIFDSIQGRKDKYGRLLAYLYI-NGTDV 133
YG + + +K+ GK VT+ + ++D+YGR+L +Y +G +V
Sbjct: 59 -------------YGRWSTDMMKSLVAGKTVTVTY----FQRDRYGRILGQVYAPDGMNV 101
Query: 134 NAELVKMGLARVFYEKKFDKIKEYSELEDEARKRRQNLWS 173
N +V+ G A V+ + D + L++EAR++++ LWS
Sbjct: 102 NQFMVRAGAAWVYEQYNTDPV--LPVLQNEARQQKRGLWS 139
>gi|10955331 hypothetical 15.6 kD protein in fino 3'region precursor; similar to
SWISS_PROT accession number Q99342 [Escherichia coli]
>gi|7435213|pir||T00303 nuclease homolog - Escherichia
coli plasmid pO157 >gi|7466667|pir||T42103 nuclease
homolog - Escherichia coli plasmid pO157
>gi|3337063|dbj|BAA31822.1| (AB011549) hypothetical 15.6
kD protein in fino 3'region precursor~similar to
SWISS_PROT accession number Q99342 [Escherichia coli]
>gi|3822117|gb|AAC70071.1| (AF074613) hypothetical
protein 15.6 kDa protein in finO 3' region precursor
[Escherichia coli O157:H7]
Length = 153
Score = 80.8 bits (196), Expect = 1e-14
Identities = 52/160 (32%), Positives = 91/160 (56%), Gaps = 29/160 (18%)
Query: 18 IALILGCLSQP---KELHGKVVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPG 74
I L+L S P ++HG+VV+V+DGDT+ V + + + V++RL+ IDAPE ++D
Sbjct: 5 IPLVLFIFSWPVLCADIHGRVVRVLDGDTIEV-MDSRKAVRIRLVNIDAPEKKQD----- 58
Query: 75 EYPGIHNLSCLVKYGYIAKEFLKNYTLGKDVTLIFDSIQGRKDKYGRLLAYLYI-NGTDV 133
YG + + +K+ GK VT+ + ++D+YGR+L +Y +G +V
Sbjct: 59 -------------YGRWSTDMMKSLVAGKTVTVTY----FQRDRYGRMLGQVYAPDGMNV 101
Query: 134 NAELVKMGLARVFYEKKFDKIKEYSELEDEARKRRQNLWS 173
N +V+ G A V+ + D + L++EAR++++ LWS
Sbjct: 102 NQFMVRAGAAWVYEQYNTDPV--LPVLQNEARQQKRGLWS 139
>pdb|1SYB| Staphylococcal Nuclease (E.C.3.1.31.1) With Residues 27 - 31
(Tyr-Lys-Gly-Gln-Pro) Replaced With Residues 160 - 165
Of Concanavalin A (Ser-Ser-Asn-Gly-Ser-Pro) Complexed
With 2'-Deoxy-3'-5'-Diphosphothymidine And Calcium
Length = 150
Score = 79.6 bits (193), Expect = 2e-14
Identities = 56/151 (37%), Positives = 79/151 (52%), Gaps = 19/151 (12%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + SNG + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKLMSSNGSPMTFRLLLVDTPETK------------HPKKGV 52
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 53 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 111
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 112 VAYVYKPNNTHEQHLRKSEAQAKKEKLNIWS 142
>sp|P43270|NUC_STAHY THERMONUCLEASE PRECURSOR (TNASE) (MICROCOCCAL NUCLEASE)
(STAPHYLOCOCCAL NUCLEASE) >gi|532653|gb|AAA26661.1|
(L23973) thermonuclease [Staphylococcus hyicus]
Length = 169
Score = 78.4 bits (190), Expect = 5e-14
Identities = 49/150 (32%), Positives = 81/150 (53%), Gaps = 16/150 (10%)
Query: 25 LSQPKELHGKVVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSC 84
LS E KV++V+DGDT+ V +G+ +R+IG+D PE ++P +
Sbjct: 34 LSNANEQTYKVIRVIDGDTIIVD-KDGKQQNLRMIGVDTPET----VKPN--------TP 80
Query: 85 LVKYGYIAKEFLKNYTLGKDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
+ YG A +F K + + V L +D + KD+YGR LAY+++ N +L K GLAR
Sbjct: 81 VQPYGKEASDFTKRHLTNQKVRLEYDKQE--KDRYGRTLAYVWLGKEMFNEKLAKEGLAR 138
Query: 145 V-FYEKKFDKIKEYSELEDEARKRRQNLWS 173
FY + + + + +A+K ++N+WS
Sbjct: 139 AKFYRPNYKYQERIEQAQKQAQKLKKNIWS 168
>gi|9630131 putative DNase/RNase endonuclease [Bacteriophage SPBc2]
>gi|7435215|pir||T12770 probable endonuclease yokF -
Bacillus subtilis phage SPBc2
>gi|2634581|emb|CAB14079.1| (Z99115) similar to
hypothetical proteins [Bacillus subtilis]
>gi|3025484|gb|AAC12979.1| (AF020713) putative
DNase/RNase endonuclease [Bacteriophage SPBc2]
Length = 296
Score = 76.1 bits (184), Expect = 2e-13
Identities = 53/141 (37%), Positives = 73/141 (51%), Gaps = 16/141 (11%)
Query: 37 KVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCLVKYGYIAKEFL 96
+ VDGDT+ V NG VR + ID PE + +P SC+ YG A +
Sbjct: 74 RAVDGDTIKVTY-NGNVDTVRYLLIDTPETK----KPN--------SCVQPYGEDASKRN 120
Query: 97 KNYTLGKDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLARV--FYEKKFDKI 154
K + L FD R+DKYGRLLAY+Y++G V L+K GLARV YE I
Sbjct: 121 KELVNSGKLQLEFDK-GDRRDKYGRLLAYVYVDGKSVQETLLKEGLARVAYVYEPNTKYI 179
Query: 155 KEYSELEDEARKRRQNLWSCN 175
++ + E EA+ + ++WS N
Sbjct: 180 DQFKKDEQEAKSEKLSIWSKN 200
>gi|10956387 pXO1-141 [Bacillus anthracis] >gi|7474251|pir||D59108 hypothetical
protein pXO1-141 - Bacillus anthracis virulence plasmid
pXO1 >gi|4894356|gb|AAD32444.1|AAD32444 (AF065404)
pXO1-141 [Bacillus anthracis]
Length = 214
Score = 74.1 bits (179), Expect = 9e-13
Identities = 52/144 (36%), Positives = 73/144 (50%), Gaps = 18/144 (12%)
Query: 33 GKVVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCLVKYGYIA 92
GKV+ + DGDT+ V + G+ VRL+ +D PE + P K G A
Sbjct: 68 GKVLHIKDGDTIDVNVK-GQKQTVRLLLLDTPESVSQKIPPQ------------KMGKEA 114
Query: 93 KEFLKNYTLGKDVTLIFDSIQGRK-DKYGRLLAYLYINGTDVNAELVKMGLARVFY--EK 149
FLK GK VTL++D QG K DKYGR LAY++ NG +N + K G + Y +
Sbjct: 115 SFFLKKQLDGKSVTLVYD--QGPKEDKYGRKLAYVFCNGIHINELMAKSGYGIIAYIFKP 172
Query: 150 KFDKIKEYSELEDEARKRRQNLWS 173
+ E E E EA++ + +WS
Sbjct: 173 NTTLLPEMLEAEKEAKEAKAGVWS 196
>pdb|1SNQ| Protein Stability In Staphylococcal Nuclease
Length = 149
Score = 74.1 bits (179), Expect = 9e-13
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HGKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKGNNTHEQLLRKSEAQAKKEKLNIWS 141
>pdb|1NSN|S Chain S, Immunoglobulin, Staphylococcal Nuclease Mol_id: 1;
Molecule: Igg Fab (Igg1, Kappa); Chain: L, H; Domain:
Fragment N10; Synonym: N10 Fab Immunoglobulin; Mol_id:
2; Molecule: Staphylococcal Nuclease; Chain: S; Synonym:
Staphylococcal Nuclease Ri>
Length = 149
Score = 73.7 bits (178), Expect = 1e-12
Identities = 55/153 (35%), Positives = 80/153 (51%), Gaps = 20/153 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWSCN 175
V Y K + E + E +A+K + N+WS N
Sbjct: 111 VAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEN 143
>pdb|1SYG| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant With Pro 117
Replaced By Ala (P117a)
Length = 149
Score = 73.7 bits (178), Expect = 1e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKANNTHEQHLRKSEAQAKKEKLNIWS 141
>pdb|1EY8|A Chain A, Structure Of S. Nuclease Stabilizing Triple Mutant
P117gH124LS128A
Length = 149
Score = 73.7 bits (178), Expect = 1e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKGNNTHEQLLRKAEAQAKKEKLNIWS 141
>pdb|1SYF| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant With Pro 117
Replaced By Thr (P117t) Complexed With
2'-Deoxy-3'-5'-Diphosphothymidine And Calcium
>gi|576292|pdb|1SYE| Staphylococcal Nuclease
(E.C.3.1.31.1) Mutant With Pro 117 Replaced By Thr
(P117t)
Length = 149
Score = 73.7 bits (178), Expect = 1e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKTNNTHEQHLRKSEAQAKKEKLNIWS 141
>pdb|1SYD| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant With Pro 117
Replaced By Gly (P117g) Complexed With
2'-Deoxy-3'-5'-Diphosphothymidine And Calcium
>gi|576290|pdb|1SYC| Staphylococcal Nuclease
(E.C.3.1.31.1) Mutant With Pro 117 Replaced By Gly
(P117g)
Length = 149
Score = 73.4 bits (177), Expect = 2e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKGNNTHEQHLRKSEAQAKKEKLNIWS 141
>pir||D69888 micrococcal nuclease homolog yncB - Bacillus subtilis
>gi|1750126|gb|AAB41095.1| (U66480) YncB [Bacillus
subtilis] >gi|2634146|emb|CAB13646.1| (Z99113) similar
to micrococcal nuclease [Bacillus subtilis]
Length = 211
Score = 73.4 bits (177), Expect = 2e-12
Identities = 52/150 (34%), Positives = 79/150 (52%), Gaps = 16/150 (10%)
Query: 26 SQPKELHGKVVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
+Q K ++ + + +DGDT+ V + NG+ VR + +D PE + +P SC+
Sbjct: 61 NQKKLVNVTLDRAIDGDTIKV-IYNGKKDTVRYLLVDTPETK----KPN--------SCV 107
Query: 86 VKYGYIAKEFLKNYTLGKDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLARV 145
YG A + K + L FD R+DKYGRLLAY+Y++G V L+K GLARV
Sbjct: 108 QPYGEDASKRNKELVNSGKLQLEFDK-GDRRDKYGRLLAYVYVDGKSVQETLLKEGLARV 166
Query: 146 --FYEKKFDKIKEYSELEDEARKRRQNLWS 173
YE I ++ E EA+ + ++WS
Sbjct: 167 AYVYEPNTKYIDQFRLDEQEAKSDKLSIWS 196
>pdb|1SNP| Protein Stability In Staphylococcal Nuclease
Length = 149
Score = 73.4 bits (177), Expect = 2e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKGNNTHEQLLRKSEAQAKKEKLNIWS 141
>pir||H81372 probable micrococcal nuclease (EC 3.1.31.1) Cj0979c [imported] -
Campylobacter jejuni (strain NCTC 11168)
>gi|6968414|emb|CAB73235.1| (AL139076) putative secreted
nuclease [Campylobacter jejuni]
Length = 175
Score = 73.4 bits (177), Expect = 2e-12
Identities = 47/159 (29%), Positives = 77/159 (47%), Gaps = 31/159 (19%)
Query: 16 ILIALILGCLSQPKELHGKVVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGE 75
+++ I ++Q GKVV+++DGDT+ V N + ++R GIDAPEL++
Sbjct: 29 LVVVFIQNYIAQNSSFEGKVVRIIDGDTIEVNHEN-KLARIRFFGIDAPELKQ------- 80
Query: 76 YPGIHNLSCLVKYGYIAKEFLKNYTLGKDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNA 135
+G +KE L GK V +I+ + KD YGR++A + +N D+N
Sbjct: 81 -----------SFGKQSKEALSRILSGKQVEIIYKN----KDTYGRIVAIVKLNDVDINR 125
Query: 136 ELVKMGL--ARVFYEKKFDKIKEYSELEDEARKRRQNLW 172
LV G A +Y Y++ ++ A+K LW
Sbjct: 126 FLVSKGYAWADTYYS------NAYTKEQENAKKNHLGLW 158
>pdb|1EY7|A Chain A, Structure Of S. Nuclease Stabilizing Mutant S128a
Length = 149
Score = 73.4 bits (177), Expect = 2e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKPNNTHEQHLRKAEAQAKKEKLNIWS 141
>pdb|1EY9|A Chain A, Structure Of S. Nuclease Stabilizing Quadruple Mutant
T41iP117GH124LS128A
Length = 149
Score = 73.4 bits (177), Expect = 2e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDIPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKGNNTHEQLLRKAEAQAKKEKLNIWS 141
>sp|P43269|NUC_STAIN THERMONUCLEASE PRECURSOR (TNASE) (MICROCOCCAL NUCLEASE)
(STAPHYLOCOCCAL NUCLEASE) >gi|282258|pir||S26079
micrococcal nuclease (EC 3.1.31.1) precursor -
Staphylococcus intermedius >gi|47146|emb|CAA47910.1|
(X67678) thermonuclease [Staphylococcus intermedius]
Length = 168
Score = 73.0 bits (176), Expect = 2e-12
Identities = 48/139 (34%), Positives = 75/139 (53%), Gaps = 16/139 (11%)
Query: 35 VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCLVKYGYIAKE 94
V +V+DGDT+ + +G+ +VRLIG+D PE ++P + + YG A
Sbjct: 43 VKRVIDGDTIIID-KDGQDERVRLIGVDTPET----VKPN--------TPVQPYGKAASN 89
Query: 95 FLKNYTLGKDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLARVFYEKKFDKI 154
F K + + V L +D + KDKYGR LAY+++ N +L K GLAR + DK
Sbjct: 90 FTKKHLTNQRVRLEYD--REPKDKYGRTLAYVWLGDEMFNVKLAKEGLARAKFYPPNDKY 147
Query: 155 KEYSE-LEDEARKRRQNLW 172
+ E + EA+K++ N+W
Sbjct: 148 RILIEQAQKEAQKKQLNIW 166
>sp|P00644|NUC_STAAU THERMONUCLEASE PRECURSOR (TNASE) (MICROCOCCAL NUCLEASE)
(STAPHYLOCOCCAL NUCLEASE) >gi|67305|pir||NCSAF
micrococcal nuclease (EC 3.1.31.1) precursor -
Staphylococcus aureus >gi|673492|emb|CAA24594.1|
(V01281) nuclease [Staphylococcus aureus]
Length = 231
Score = 73.0 bits (176), Expect = 2e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 87 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 133
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 134 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 192
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 193 VAYVYKPNNTHEQHLRKSEAQAKKEKLNIWS 223
>prf||1109959A nuclease,staphylococcal [Staphylococcus aureus]
Length = 242
Score = 73.0 bits (176), Expect = 2e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 98 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 144
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 145 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 203
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 204 VAYVYKPNNTHEQHLRKSEAQAKKEKLNIWS 234
>pdb|1EY0|A Chain A, Structure Of Wild-Type S. Nuclease At 1.6 A Resolution
>gi|11514489|pdb|1EYD|A Chain A, Structure Of Wild-Type
S. Nuclease At 1.7 A Resolution >gi|576287|pdb|1STN|
Staphylococcal Nuclease (E.C.3.1.31.1)
>gi|999672|pdb|1STG| Staphylococcal Nuclease
(E.C.3.1.31.1) >gi|999674|pdb|1STH| Staphylococcal
Nuclease (E.C.3.1.31.1) Complexed With Co(Ii) Ion And
Thymidine 3',5'-Bisphosphate (Pdtp) >gi|230331|pdb|1SNC|
Staphylococcal Nuclease (E.C.3.1.31.1) Complex With A
Calcium Ion And 3-Prime, 5-Prime-Deoxythymidine
Bisphosphate >gi|229342|prf||710414A nuclease
[Staphylococcus aureus]
Length = 149
Score = 73.0 bits (176), Expect = 2e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKPNNTHEQHLRKSEAQAKKEKLNIWS 141
>pdb|1STY| Staphylococcal Nuclease (E.C.3.1.31.1) Insertion Mutant With
Glycine Residue Inserted In An Alpha Helix, Between
Arg126 And Lys127 (126g127) Complex With Calcium And
Inhibitor Thymidine 3',5'-Bisphosphate)
Length = 150
Score = 73.0 bits (176), Expect = 2e-12
Identities = 54/152 (35%), Positives = 79/152 (51%), Gaps = 21/152 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKEY---SELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKPNNTHEQHLRGKSEAQAKKEKLNIWS 142
>pdb|1SNO| Protein Stability In Staphylococcal Nuclease
Length = 149
Score = 73.0 bits (176), Expect = 2e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKPNNTHEQLLRKSEAQAKKEKLNIWS 141
>gb|AAC14660.1| (AF038666) deltaSP-Nuc [Cloning vector pFUN]
Length = 155
Score = 73.0 bits (176), Expect = 2e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 11 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 57
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 58 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 116
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 117 VAYVYKPNNTHEQHLRKSEAQAKKEKLNIWS 147
>pdb|1EYA|A Chain A, Structure Of S. Nuclease Stabilizing Quintuple Mutant
T33vT41IP117GH124LS128A
Length = 149
Score = 73.0 bits (176), Expect = 2e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMVFRLLLVDIPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKGNNTHEQLLRKAEAQAKKEKLNIWS 141
>pdb|1EYC|A Chain A, Structure Of S. Nuclease Stabilizing Quintuple Mutant
T41iS59AP117GH124LS128A
Length = 149
Score = 73.0 bits (176), Expect = 2e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDIPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEAAAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKGNNTHEQLLRKAEAQAKKEKLNIWS 141
>pdb|1EZ6|A Chain A, Structure Of S. Nuclease Stabilizing Sextuple Mutant
T33vT41IS59AP117GH124LS128A
Length = 149
Score = 72.6 bits (175), Expect = 3e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMVFRLLLVDIPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEAAAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKGNNTHEQLLRKAEAQAKKEKLNIWS 141
>pdb|2SNM| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant With Val 66 Replaced
By Lys (V66k)
Length = 149
Score = 72.6 bits (175), Expect = 3e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMKENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKPNNTHEQHLRKSEAQAKKEKLNIWS 141
>pdb|1KDB| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant With Lys 116
Replaced By Glu (K116e)
Length = 149
Score = 72.6 bits (175), Expect = 3e-12
Identities = 54/153 (35%), Positives = 79/153 (51%), Gaps = 20/153 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 V--FYEKKFDKIKEYSELEDEARKRRQNLWSCN 175
V YE + + E +A+K + N+WS N
Sbjct: 111 VAYVYEPNNTHEQHLRKSEAQAKKEKLNIWSEN 143
>pdb|1EY4|A Chain A, Structure Of S. Nuclease Stabilizing Mutant S59a
Length = 149
Score = 72.6 bits (175), Expect = 3e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEAAAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKPNNTHEQHLRKSEAQAKKEKLNIWS 141
>pdb|1EY5|A Chain A, Structure Of S. Nuclease Stabilizing Mutant T33v
>gi|11514493|pdb|1EZ8|A Chain A, Structure Of S.
Nuclease Stabilizing Mutant T33v
Length = 149
Score = 72.6 bits (175), Expect = 3e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMVFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKPNNTHEQHLRKSEAQAKKEKLNIWS 141
>pdb|1EY6|A Chain A, Structure Of S. Nuclease Stabilizing Mutant T41i
Length = 149
Score = 72.6 bits (175), Expect = 3e-12
Identities = 54/151 (35%), Positives = 79/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDIPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKPNNTHEQHLRKSEAQAKKEKLNIWS 141
>pdb|1STA| Staphylococcal Nuclease (E.C.3.1.31.1) Double Insertion Mutant
With Two Glycine Residues Inserted In The First Beta
Strand Between Pro 11 And Ala 12 (Ins(P11-Gg)) Complexed
With Calcium And The Inhibitor Thymidine
3',5'-Diphosphate
Length = 151
Score = 72.2 bits (174), Expect = 4e-12
Identities = 54/153 (35%), Positives = 79/153 (51%), Gaps = 22/153 (14%)
Query: 29 KELHGK-----VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLS 83
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H
Sbjct: 5 KKLHKEPGGATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKK 51
Query: 84 CLVKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGL 142
+ KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GL
Sbjct: 52 GVEKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGL 110
Query: 143 ARVFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
A+V Y K + E + E +A+K + N+WS
Sbjct: 111 AKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWS 143
>pdb|1SNM| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant (Glu 43 Replaced By
Asp) (E43D) Complex With A Calcium Ion And 3-Prime,
5-Prime-Deoxythymidine Bisphosphate
Length = 149
Score = 71.8 bits (173), Expect = 5e-12
Identities = 53/151 (35%), Positives = 79/151 (52%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D P+ + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPDTK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKPNNTHEQHLRKSEAQAKKEKLNIWS 141
>pdb|2SNS| Staphylococcal Nuclease (E.C.3.1.33.1) Complex With
2(Prime)-Deoxy-3(Prime)-5(Prime)-Diphosphothymidine
Length = 149
Score = 71.8 bits (173), Expect = 5e-12
Identities = 54/153 (35%), Positives = 80/153 (51%), Gaps = 20/153 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + F+ Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFNKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWSCN 175
V Y K + E + E +A+K + N+WS N
Sbjct: 111 VAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEN 143
>pdb|1KDC| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant With Lys 116
Replaced By Asn (K116n)
Length = 149
Score = 71.8 bits (173), Expect = 5e-12
Identities = 54/153 (35%), Positives = 79/153 (51%), Gaps = 20/153 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWSCN 175
V Y + E + E +A+K + N+WS N
Sbjct: 111 VAYVYNPNNTHEQHLRKSEAQAKKEKLNIWSEN 143
>pdb|1SND|A Chain A, Staphylococcal Nuclease Dimer Containing A Deletion Of
Residues 114-119 Complexed With Calcium Chloride And The
Competitive Inhibitor Deoxythymidine-3',5'-Diphosphate
>gi|2098291|pdb|1SND|B Chain B, Staphylococcal Nuclease
Dimer Containing A Deletion Of Residues 114-119
Complexed With Calcium Chloride And The Competitive
Inhibitor Deoxythymidine-3',5'-Diphosphate
Length = 143
Score = 71.8 bits (173), Expect = 5e-12
Identities = 52/149 (34%), Positives = 78/149 (51%), Gaps = 22/149 (14%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKEYSELEDEARKRRQNLWS 173
V Y + + + E +A+K + N+WS
Sbjct: 111 VAYTHE----QHLRKSEAQAKKEKLNIWS 135
>pdb|1ENC| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant With Asp 21 Replaced
By Glu (D21e) Complexed With A Calcium Ion And The
Inhibitor Thymidine 3',5'-Diphosphate
Length = 149
Score = 71.4 bits (172), Expect = 6e-12
Identities = 53/151 (35%), Positives = 79/151 (52%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DG+TV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGETVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKPNNTHEQHLRKSEAQAKKEKLNIWS 141
>pdb|1KDA| Staphylococcal Nuclease (E.C.3.1.31.1) Mutant With Lys 116
Replaced By Asp (K116d)
Length = 149
Score = 71.4 bits (172), Expect = 6e-12
Identities = 53/153 (34%), Positives = 79/153 (50%), Gaps = 20/153 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 V--FYEKKFDKIKEYSELEDEARKRRQNLWSCN 175
V Y+ + + E +A+K + N+WS N
Sbjct: 111 VAYVYDPNNTHEQHLRKSEAQAKKEKLNIWSEN 143
>pdb|3NUC| Staphlococcal Nuclease, 1-N-Propane Thiol Disulfide To V23c
Variant >gi|3212273|pdb|1A2T| Staphylococcal Nuclease,
B-Mercaptoethanol Disulfide To V23c Variant
>gi|3212274|pdb|1A2U| Staphylococcal Nuclease, V23c
Variant, Complex With 1-N-Butane Thiol And
3',5'-Thymidine Diphosphate >gi|2981899|pdb|2NUC|
Staphlococcal Nuclease, Ethane Thiol Disulfide To V23c
Variant >gi|3212326|pdb|1A3U| Staphylococcal Nuclease,
Cyclohexane Thiol Disulfide To V23c Variant
>gi|3212325|pdb|1A3T| Staphylococcal Nuclease, V23c
Variant, Complex With 2-Fluoroethane Thiol And
3',5'-Thymidine Diphosphate >gi|2392077|pdb|1AEX|
Staphylococcal Nuclease, Methane Thiol Disulfide To V23c
Variant >gi|3212829|pdb|5NUC| Staphylococcal Nuclease,
1-N-Pentane Thiol Disulfide To V23c Variant
>gi|3212327|pdb|1A3V| Staphylococcal Nuclease,
Cyclopentane Thiol Disulfide To V23c Variant
Length = 149
Score = 71.0 bits (171), Expect = 8e-12
Identities = 53/151 (35%), Positives = 78/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDT + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTXKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKPNNTHEQHLRKSEAQAKKEKLNIWS 141
>pdb|1NUC| Staphylococcal Nuclease, V23c Variant
Length = 149
Score = 71.0 bits (171), Expect = 8e-12
Identities = 53/151 (35%), Positives = 78/151 (51%), Gaps = 20/151 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCL 85
K+LH + ++K +DGDT + + G+ + RL+ +D PE + H +
Sbjct: 5 KKLHKEPATLIKAIDGDTCKL-MYKGQPMTFRLLLVDTPETK------------HPKKGV 51
Query: 86 VKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLAR 144
KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+
Sbjct: 52 EKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAK 110
Query: 145 VFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
V Y K + E + E +A+K + N+WS
Sbjct: 111 VAYVYKPNNTHEQHLRKSEAQAKKEKLNIWS 141
>pdb|2ENB| Staphylococcal Nuclease (E.C.3.1.31.1) Mutation With Asp 21
Replaced By Glu (D21e) Complexed With The Inhibitor
Thymidine 3',5'-Diphosphate >gi|576099|pdb|1ENA|
Staphylococcal Nuclease (E.C.3.1.31.1) Mutation With Asp
21 Replaced By Glu (D21e) Complexed With A Calcium Ion
Length = 135
Score = 70.2 bits (169), Expect = 1e-11
Identities = 50/142 (35%), Positives = 74/142 (51%), Gaps = 17/142 (11%)
Query: 35 VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCLVKYGYIAKE 94
++K +DG+TV + + G+ + RL+ +D PE + H + KYG A
Sbjct: 8 LIKAIDGETVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGVEKYGPEASA 54
Query: 95 FLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLARVFYEKKFDK 153
F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+V Y K +
Sbjct: 55 FTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNN 113
Query: 154 IKE--YSELEDEARKRRQNLWS 173
E + E +A+K + N+WS
Sbjct: 114 THEQHLRKSEAQAKKEKLNIWS 135
>pdb|1KAA| Staphylococcal Nuclease (E.C.3.1.33.1) Mutant With Lys 116
Replaced By Ala (K116a)
Length = 136
Score = 69.5 bits (167), Expect = 2e-11
Identities = 50/142 (35%), Positives = 73/142 (51%), Gaps = 17/142 (11%)
Query: 35 VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCLVKYGYIAKE 94
++K +DGDTV + + G+ + RL+ +D PE + H + KYG A
Sbjct: 9 LIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGVEKYGPEASA 55
Query: 95 FLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLARVFYEKKFDK 153
F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+V Y +
Sbjct: 56 FTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYAPNN 114
Query: 154 IKE--YSELEDEARKRRQNLWS 173
E + E +A+K + N+WS
Sbjct: 115 THEQHLRKSEAQAKKEKLNIWS 136
>pdb|1KAB| Staphylococcal Nuclease (E.C.3.1.33.1) Mutant With Lys 116
Replaced By Gly (K116g)
Length = 136
Score = 69.1 bits (166), Expect = 3e-11
Identities = 50/142 (35%), Positives = 73/142 (51%), Gaps = 17/142 (11%)
Query: 35 VVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGEYPGIHNLSCLVKYGYIAKE 94
++K +DGDTV + + G+ + RL+ +D PE + H + KYG A
Sbjct: 9 LIKAIDGDTVKL-MYKGQPMTFRLLLVDTPETK------------HPKKGVEKYGPEASA 55
Query: 95 FLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLARVFYEKKFDK 153
F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA+V Y +
Sbjct: 56 FTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYGPNN 114
Query: 154 IKE--YSELEDEARKRRQNLWS 173
E + E +A+K + N+WS
Sbjct: 115 THEQHLRKSEAQAKKEKLNIWS 136
>pdb|1STB| Staphylococcal Nuclease (E.C.3.1.31.1) Insertion Mutant With Leu
Inserted At The End Of The Third Beta-Strand Between Leu
36 And Leu 37 (Ins(L36-L)) Complexed With Thymidine
3',5'-Diphosphate And Calcium
Length = 150
Score = 68.3 bits (164), Expect = 5e-11
Identities = 54/152 (35%), Positives = 79/152 (51%), Gaps = 21/152 (13%)
Query: 29 KELHGK---VVKVVDGDTVYVKLSNGETVKVRLIG-IDAPELEEDLMRPGEYPGIHNLSC 84
K+LH + ++K +DGDTV + + G+ + RL+ +D PE + H
Sbjct: 5 KKLHKEPATLIKAIDGDTVKL-MYKGQPMTFRLLLLVDTPETK------------HPKKG 51
Query: 85 LVKYGYIAKEFLKNYTLG-KDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNAELVKMGLA 143
+ KYG A F K K + + FD Q R DKYGR LAY+Y +G VN LV+ GLA
Sbjct: 52 VEKYGPEASAFTKKMVENAKKIEVEFDKGQ-RTDKYGRGLAYIYADGKMVNEALVRQGLA 110
Query: 144 RVFYEKKFDKIKE--YSELEDEARKRRQNLWS 173
+V Y K + E + E +A+K + N+WS
Sbjct: 111 KVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWS 142
>gi|10957342 putative partition protein [Salmonella typhi]
>gi|7800395|gb|AAF69991.1|AF250878_152 (AF250878)
putative partition protein [Salmonella typhi]
Length = 171
Score = 68.3 bits (164), Expect = 5e-11
Identities = 42/157 (26%), Positives = 74/157 (46%), Gaps = 22/157 (14%)
Query: 16 ILIALILGCLSQPKELHGKVVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLMRPGE 75
+++ L+ KVVK++DGDT+ ++K+R+ GIDAPE ++
Sbjct: 12 LIVLFPAAVLAGQSSFEAKVVKIIDGDTITALDGQNTSIKIRMYGIDAPESKQ------- 64
Query: 76 YPGIHNLSCLVKYGYIAKEFLKNYTLGKDVTLIFDSIQGRKDKYGRLLAYLYINGTDVNA 135
+G AK+ L + +++T+ I D YGR+L ++++G D+NA
Sbjct: 65 -----------AFGQKAKQALSSAIAAQNITV----IDHGPDIYGRMLGTIWLDGYDINA 109
Query: 136 ELVKMGLARVFYEKKFDKIKEYSELEDEARKRRQNLW 172
+V G A V+ + Y + E A+K + LW
Sbjct: 110 SMVDSGYAWVYRFDGNAIVPNYLKFEASAQKAVKGLW 146
>sp|Q57519|YC96_HAEIN HYPOTHETICAL PROTEIN HI1296 PRECURSOR >gi|1075106|pir||A64115 parB
protein homolog - Haemophilus influenzae (strain Rd
KW20) >gi|1574754|gb|AAC22942.1| (U32809) nuclease,
putative [Haemophilus influenzae Rd]
Length = 178
Score = 67.5 bits (162), Expect = 9e-11
Identities = 48/164 (29%), Positives = 81/164 (49%), Gaps = 25/164 (15%)
Query: 12 VSSIILIALILGCLSQPKELHGKVVKVVDGDTVYVKLSNGETVKVRLIGIDAPELEEDLM 71
++S++LI +L S+ K +VVK+ DGDT+ +++KVRL IDAPE +
Sbjct: 8 LTSLLLIFTVLSACSREKNTC-RVVKISDGDTLTCLTKGNKSIKVRLAEIDAPEKSQ--- 63
Query: 72 RPGEYPGIHNLSCLVKYGYIAKEFLKNYTLGKDVTLIFDSIQGRKDKYGRLLAYLYINGT 131
+G +K+ L + K+V L + KD+Y R LA +Y
Sbjct: 64 ---------------AFGQKSKKTLSDLVYQKNVRL----ARKGKDRYQRTLAVVYYQKQ 104
Query: 132 DVNAELVKMGLARVFYEKKFDKIKEYSELEDEARKRRQNLWSCN 175
++N E+VK G+A + + D I Y + ++ A+ + LW+ N
Sbjct: 105 NINLEMVKQGMAWAYKQYSHDPI--YLQAQENAQAKGIGLWADN 146
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.321 0.142 0.410
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65860432
Number of Sequences: 2977
Number of extensions: 2775265
Number of successful extensions: 8171
Number of sequences better than 1.0e-10: 51
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 48
Number of HSP's that attempted gapping in prelim test: 8137
Number of HSP's gapped (non-prelim): 51
length of query: 175
length of database: 189,106,746
effective HSP length: 52
effective length of query: 123
effective length of database: 157,985,422
effective search space: 19432206906
effective search space used: 19432206906
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 162 (67.5 bits)