BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB0721 (PAB0721) DE:METHLYTRANSFERASE (311 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||C75086 methlytransferase PAB0721 - Pyrococcus abyssi (strai... 622 e-177 pir||H71054 hypothetical protein PH1134 - Pyrococcus horikoshii ... 467 e-131 pir||D72272 conserved hypothetical protein - Thermotoga maritima... 265 7e-70 sp|Q58523|YB23_METJA HYPOTHETICAL PROTEIN MJ1123 >gi|2128680|pir... 214 9e-55 gi|11498396 conserved hypothetical protein [Archaeoglobus fulgid... 126 4e-28 emb|CAC11165.1| (AL445063) hypothetical protein [Thermoplasma ac... 122 4e-27 pir||A72576 hypothetical protein APE1889 - Aeropyrum pernix (str... 120 3e-26 pir||H70409 conserved hypothetical protein aq_1270 - Aquifex aeo... 120 3e-26 sp|Q10853|YK03_MYCTU HYPOTHETICAL 31.1 KDA PROTEIN RV2003C >gi|7... 99 6e-20 gb|AAK42414.1| Methyltransferase, putative [Sulfolobus solfatari... 87 3e-16 >pir||C75086 methlytransferase PAB0721 - Pyrococcus abyssi (strain Orsay) >gi|5458504|emb|CAB49992.1| (AJ248286) METHLYTRANSFERASE [Pyrococcus abyssi] Length = 311 Score = 622 bits (1587), Expect = e-177 Identities = 311/311 (100%), Positives = 311/311 (100%) Query: 1 MITMPKIRPFEEYTERYENWFEKHRYAYLSEINAIKSVMPQEECVEVGVGSGRFAEPLGI 60 MITMPKIRPFEEYTERYENWFEKHRYAYLSEINAIKSVMPQEECVEVGVGSGRFAEPLGI Sbjct: 1 MITMPKIRPFEEYTERYENWFEKHRYAYLSEINAIKSVMPQEECVEVGVGSGRFAEPLGI 60 Query: 61 KLGVEPSKRMAKIAESRGIKVIEGVAEDLPFPDNSLECILMVTTICFVDDPEKAIKEAYR 120 KLGVEPSKRMAKIAESRGIKVIEGVAEDLPFPDNSLECILMVTTICFVDDPEKAIKEAYR Sbjct: 61 KLGVEPSKRMAKIAESRGIKVIEGVAEDLPFPDNSLECILMVTTICFVDDPEKAIKEAYR 120 Query: 121 VLKPNGHIIIGFIDRESKIGREYEKNKDKSVFYREANFFSTQEIVNLLERNGFKVEKIVQ 180 VLKPNGHIIIGFIDRESKIGREYEKNKDKSVFYREANFFSTQEIVNLLERNGFKVEKIVQ Sbjct: 121 VLKPNGHIIIGFIDRESKIGREYEKNKDKSVFYREANFFSTQEIVNLLERNGFKVEKIVQ 180 Query: 181 TLFRRLEEVDSVEPVKEGFGEGSFVAIRARKMDAMDFAEKVKGLAERLGEDKLREAIDRF 240 TLFRRLEEVDSVEPVKEGFGEGSFVAIRARKMDAMDFAEKVKGLAERLGEDKLREAIDRF Sbjct: 181 TLFRRLEEVDSVEPVKEGFGEGSFVAIRARKMDAMDFAEKVKGLAERLGEDKLREAIDRF 240 Query: 241 LTLNEGIEKTRGEHFAKAGIYGFLEGILTTLKLKHEDEEISELLSLIKKAREIEEAFLRK 300 LTLNEGIEKTRGEHFAKAGIYGFLEGILTTLKLKHEDEEISELLSLIKKAREIEEAFLRK Sbjct: 241 LTLNEGIEKTRGEHFAKAGIYGFLEGILTTLKLKHEDEEISELLSLIKKAREIEEAFLRK 300 Query: 301 GNPPIFEQKPL 311 GNPPIFEQKPL Sbjct: 301 GNPPIFEQKPL 311 >pir||H71054 hypothetical protein PH1134 - Pyrococcus horikoshii >gi|3257551|dbj|BAA30234.1| (AP000005) 310aa long hypothetical protein [Pyrococcus horikoshii] Length = 310 Score = 467 bits (1189), Expect = e-131 Identities = 219/307 (71%), Positives = 269/307 (87%), Gaps = 1/307 (0%) Query: 2 ITMPKIRPFEEYTERYENWFEKHRYAYLSEINAIKSVMPQEECVEVGVGSGRFAEPLGIK 61 I MPKI+PFEE+TERY++WFE+H+YAYLSE+NAIK V+P+EEC+EVGVG+GRFA+PLGIK Sbjct: 4 IIMPKIKPFEEHTERYDSWFERHKYAYLSELNAIKKVLPEEECIEVGVGTGRFAQPLGIK 63 Query: 62 LGVEPSKRMAKIAESRGIKVIEGVAEDLPFPDNSLECILMVTTICFVDDPEKAIKEAYRV 121 +GVEPS++MA+IAE RGIKVIEG+AEDLPFPD+SL CILMVTTICFVDD EK+IKEAYRV Sbjct: 64 IGVEPSRKMAEIAEKRGIKVIEGIAEDLPFPDSSLSCILMVTTICFVDDVEKSIKEAYRV 123 Query: 122 LKPNGHIIIGFIDRESKIGREYEKNKDKSVFYREANFFSTQEIVNLLERNGFKVEKIVQT 181 LKP G+I+IGFID+ SKIG+EYE+NKDKSVFYREA FFST+E++++LER+GFKVEKIVQT Sbjct: 124 LKPGGYIVIGFIDKNSKIGKEYERNKDKSVFYREAKFFSTEEVISILERSGFKVEKIVQT 183 Query: 182 LFRRLEEVDSVEPVKEGFGEGSFVAIRARKMDAMD-FAEKVKGLAERLGEDKLREAIDRF 240 LF +L+E+ +EPVKEG+GEGSFVAIRA+K+D + ++VK LA LGE++ AIDRF Sbjct: 184 LFHKLDEIKDIEPVKEGYGEGSFVAIRAKKVDITERIIKEVKELARDLGEEETLNAIDRF 243 Query: 241 LTLNEGIEKTRGEHFAKAGIYGFLEGILTTLKLKHEDEEISELLSLIKKAREIEEAFLRK 300 L LN+G+EK RGEHF + GIYGFLEG+LTT K K+ DE I LLS +++ RE EE FLRK Sbjct: 244 LLLNKGLEKIRGEHFVRVGIYGFLEGLLTTFKSKYNDERIDRLLSAVRELREREEYFLRK 303 Query: 301 GNPPIFE 307 NPPI E Sbjct: 304 SNPPISE 310 >pir||D72272 conserved hypothetical protein - Thermotoga maritima (strain MSB8) >gi|4981851|gb|AAD36367.1|AE001784_9 (AE001784) conserved hypothetical protein [Thermotoga maritima] Length = 207 Score = 265 bits (669), Expect = 7e-70 Identities = 120/202 (59%), Positives = 160/202 (78%) Query: 10 FEEYTERYENWFEKHRYAYLSEINAIKSVMPQEECVEVGVGSGRFAEPLGIKLGVEPSKR 69 FE + YE WF HR+AYLSE+ A+K ++P+ VE+GVG+GRFA PL IK+GVEPS+R Sbjct: 5 FERFVNEYERWFLVHRFAYLSELQAVKCLLPEGRGVEIGVGTGRFAVPLKIKIGVEPSER 64 Query: 70 MAKIAESRGIKVIEGVAEDLPFPDNSLECILMVTTICFVDDPEKAIKEAYRVLKPNGHII 129 MA+IA RG+ V++G AE+LP D S + LMVTTICFVDDPE+A+KEAYR+LK G++I Sbjct: 65 MAEIARKRGVFVLKGTAENLPLKDESFDFALMVTTICFVDDPERALKEAYRILKKGGYLI 124 Query: 130 IGFIDRESKIGREYEKNKDKSVFYREANFFSTQEIVNLLERNGFKVEKIVQTLFRRLEEV 189 +G +DRES +GREYEKNK+KSVFY+ A FFST+E+++L+ + GF+ K+VQTLF+ E+ Sbjct: 125 VGIVDRESFLGREYEKNKEKSVFYKNARFFSTEELMDLMRKAGFEEFKVVQTLFKHPSEL 184 Query: 190 DSVEPVKEGFGEGSFVAIRARK 211 +EPVKEG+GEG+FV IR K Sbjct: 185 SEIEPVKEGYGEGAFVVIRGTK 206 >sp|Q58523|YB23_METJA HYPOTHETICAL PROTEIN MJ1123 >gi|2128680|pir||B64440 hypothetical protein MJ1123 - Methanococcus jannaschii >gi|1499975|gb|AAB99125.1| (U67555) conserved hypothetical protein [Methanococcus jannaschii] Length = 205 Score = 214 bits (540), Expect = 9e-55 Identities = 105/203 (51%), Positives = 145/203 (70%), Gaps = 1/203 (0%) Query: 10 FEEYTERYENWFEKHRYAYLSEINAIKSVMPQEECVEVGVGSGRFAEPLGIKLGVEPSKR 69 F++Y E Y+ WF+++ Y SEI A+K +P+ +E+GVG+GRFA+P IK+GV+ SK Sbjct: 4 FDKYAEEYDKWFDENEIIYKSEIEALKRHIPKGRGLEIGVGTGRFAKPFNIKIGVDISKE 63 Query: 70 MAKIAESRGIKVIEGVAEDLPFPDNSLECILMVTTICFVDDPEKAIKEAYRVLKPNGHII 129 MAKIAE RGIKVI EDLPF D + L+ T + F ++P+K I+EA RVLK G II Sbjct: 64 MAKIAEKRGIKVIIAKGEDLPFKDEEFDFFLINTVLEFAENPKKMIEEAKRVLKRGGKII 123 Query: 130 IGFIDRESKIGREYEKNKDKSVFYREANFFSTQEIVNLLERNGFKVEKIVQTLFRRLEEV 189 IG IDR+S +G+ YE+ K KS FY++ANF S +E++ +L+ GFK K QT+F+ +++V Sbjct: 124 IGIIDRDSFLGKMYEEKKQKSKFYKDANFLSAKEVIEMLKELGFKNIKATQTIFKEIDKV 183 Query: 190 DSVEPVKEGFGEGSFVAIRARKM 212 D VE VKEG+GEG FVAI A K+ Sbjct: 184 DKVE-VKEGYGEGGFVAISAEKI 205 >gi|11498396 conserved hypothetical protein [Archaeoglobus fulgidus] >gi|7429712|pir||F69348 conserved hypothetical protein AF0790 - Archaeoglobus fulgidus >gi|2649819|gb|AAB90450.1| (AE001050) conserved hypothetical protein [Archaeoglobus fulgidus] Length = 184 Score = 126 bits (313), Expect = 4e-28 Identities = 69/169 (40%), Positives = 105/169 (61%), Gaps = 7/169 (4%) Query: 10 FEEYTERYENWFEKHRYAYLSEINAIKSVMPQEECVEVGVGSGRFAEPLGIKLGVEPSKR 69 F ERY+ W++++R Y +E+ + P VEVGVG+GRFA +GI LGV+ S + Sbjct: 4 FNRNAERYDAWYDRNRELYEAELELLPK--PISPSVEVGVGTGRFAV-IGIDLGVDVSLQ 60 Query: 70 MAKIAESRGIKVIEGVAEDLPFPDNSLECILMVTTICFVDDPEKAIKEAYRVLKPNGHII 129 M +IA+ RG++ + G A LPF D S ++ T+CF+D+P KA+ E RVL+ G ++ Sbjct: 61 MLRIAKKRGVECVAGDAAHLPFKDKSFRSAYLIFTLCFLDEPIKALMEIKRVLE--GTLV 118 Query: 130 IGFIDRESKIGREYEKNKDKSVFYREANFFSTQEIVNLLERNGFKVEKI 178 + S +GREY KD S FYR+A F + E++++L GFKV++I Sbjct: 119 ACVVPANSGLGREY-SGKDSS-FYRKARFLTEDEVLSMLRETGFKVKEI 165 >emb|CAC11165.1| (AL445063) hypothetical protein [Thermoplasma acidophilum] Length = 188 Score = 122 bits (304), Expect = 4e-27 Identities = 66/173 (38%), Positives = 101/173 (58%), Gaps = 9/173 (5%) Query: 7 IRPFEEYTERYENWFEKHRYAYLSEINAIKSVMPQEECVEVGVGSGRFAEPLGIKLGVEP 66 I+ F+E + +Y+NW+E HR Y ++ I +P VE+GVG+GRFA LGIK+GV+ Sbjct: 2 IKYFDENSIKYDNWYESHRKEYGDQVEFIGGRLPSGMGVEIGVGTGRFASKLGIKVGVDV 61 Query: 67 SKRMAKIAESRGIKVIEGVAEDLPFPDNSLECILMVTTICFVDDPEKAIKEAYRVLKPNG 126 S++M IA RGI I A+ LPF + + + TICF+DDP +++EA RV + Sbjct: 62 SEKMLSIASKRGIFPIMASADHLPFISGYFDFSIFIVTICFLDDPVSSLREARRVSRTVI 121 Query: 127 HIIIGFIDRESKIGREY--EKNKDKSVFYREANFFSTQEIVNLLERNGFKVEK 177 ++I + G EY + ++K FY A F++ E+V + E+ G KV K Sbjct: 122 SVLI-------EAGTEYSMKLQREKKGFYAFARFYTEDEVVGMYEKAGMKVLK 167 >pir||A72576 hypothetical protein APE1889 - Aeropyrum pernix (strain K1) >gi|5105581|dbj|BAA80894.1| (AP000062) 224aa long hypothetical protein [Aeropyrum pernix] Length = 224 Score = 120 bits (297), Expect = 3e-26 Identities = 71/207 (34%), Positives = 110/207 (52%), Gaps = 5/207 (2%) Query: 10 FEEYTERYENWFEKHRYAYLSEINAIKSVMP--QEECVEVGVGSGRFAEPLGIKLGVEPS 67 F+ ERY+ W+E++R +E + + +EVGVG+G FA +G G++PS Sbjct: 14 FDRLYERYDKWYEENRLLAANEAKTVSRALEGSPRPLLEVGVGTGFFASIVGADAGLDPS 73 Query: 68 KRMAKIAESRGIKV-IEGVAEDLPFPDNSLECILMVTTICFVDDPEKAIKEAYRVLKPNG 126 M + A RGI + + GV E +PF L+V T+CF DDP++ ++E +RVL G Sbjct: 74 LGMLRRARERGIPLLVAGVGERMPFRSRVFGSALIVVTLCFADDPQELLREVWRVLAWGG 133 Query: 127 HIIIGFIDRESKIGREYE-KNKDKSVFYREANFFSTQEIVNLLERNGFKVEKIVQTL-FR 184 ++ + R+S G YE K + FY +A F + + +L+ GF +E+ TL F+ Sbjct: 134 VLVSCIVPRDSSWGEYYEAKGRAGHPFYSKARFITNEMHHTMLKNAGFSLERQYATLSFK 193 Query: 185 RLEEVDSVEPVKEGFGEGSFVAIRARK 211 EE EPV+ G+ F RARK Sbjct: 194 PWEEPREEEPVEAWGGDYGFTCTRARK 220 >pir||H70409 conserved hypothetical protein aq_1270 - Aquifex aeolicus >gi|2983718|gb|AAC07281.1| (AE000732) hypothetical protein [Aquifex aeolicus] Length = 200 Score = 120 bits (297), Expect = 3e-26 Identities = 74/176 (42%), Positives = 106/176 (60%), Gaps = 11/176 (6%) Query: 43 ECVEVGVGSGRFAEPLGIKLGVEPSKRMAKIAESRGIKVIEGVAEDLPFPDNSLECILMV 102 E G G+GRFA+ LGI+ GV+ S M K+A+ RGIKV E+LPF + + + +V Sbjct: 27 EIFRSGRGTGRFAKALGIEYGVDTSFNMLKVAKERGIKVALAKGENLPFKSETFDSVFLV 86 Query: 103 TTICFVDDPEKAIKEAYRVLKPNGHIIIGFIDRESKIGREY-EKNKDKSVFYREANFFST 161 TICFV++P++ +KEA+RVLK +G + +G I +ESK + Y EK K+ FY+ A F+S Sbjct: 87 VTICFVENPKQVLKEAHRVLKRDGKLYLGLILKESKWAKFYEEKAKNGHPFYKHARFYSF 146 Query: 162 QEIVNLLERNGFKVEKIVQTLFRRLEEVDSVEPVKE-----GFGEGS-FVAIRARK 211 E+ ++ ++ F E++ TL LEE EPVK GF E S F IR K Sbjct: 147 FELKEMV-KDIFSFEEMKSTL---LEEPQDSEPVKNKEIKPGFYENSGFTCIRLSK 198 >sp|Q10853|YK03_MYCTU HYPOTHETICAL 31.1 KDA PROTEIN RV2003C >gi|7429714|pir||A70759 hypothetical protein Rv2003c - Mycobacterium tuberculosis (strain H37RV) >gi|1403450|emb|CAA98385.1| (Z74025) hypothetical protein Rv2003c [Mycobacterium tuberculosis] Length = 285 Score = 99.1 bits (243), Expect = 6e-20 Identities = 65/201 (32%), Positives = 105/201 (51%), Gaps = 9/201 (4%) Query: 17 YENWFEKH--RYAYLSEINAIKSVMP--QEECVEVGVGSGRFAEPLGIKLGVEPSKRMAK 72 Y+ W++ R +E+ A++ ++ + +E+GVG+GRFA+ LG++ G++PS+ Sbjct: 70 YDAWYDSPTGRPILATEVAALRPLIEVFAQPRLEIGVGTGRFADLLGVRFGLDPSRDALM 129 Query: 73 IAESRGIKVIEGVAEDLPFPDNSLECILMVTTICFVDDPEKAIKEAYRVLKPNGHIIIGF 132 A RG+ V V E +PF +LM T+CFV DP +E R+L G ++IGF Sbjct: 130 FARRRGVLVANAVGEAVPFVSRHFGAVLMAFTLCFVTDPAAIFRETRRLLADGGGLVIGF 189 Query: 133 IDRESKIGREYEKNKDK-SVFYREANFFSTQEIVNLLERNGFKVEKIVQTLFR--RLEEV 189 + R + Y + YR+A F++ E+ LL +GF+V TL + L Sbjct: 190 LPRGTPWADLYALRAARGQPGYRDARFYTAAELEQLLADSGFRVIARRCTLHQPPGLARY 249 Query: 190 DSVEPVKEGFGEGS-FVAIRA 209 D +E +G G+ FVAI A Sbjct: 250 D-IEAAHDGIQAGAGFVAISA 269 >gb|AAK42414.1| Methyltransferase, putative [Sulfolobus solfataricus] Length = 223 Score = 86.6 bits (211), Expect = 3e-16 Identities = 62/198 (31%), Positives = 99/198 (49%), Gaps = 11/198 (5%) Query: 17 YENWFEKHRYAYLSEINAIKSVMPQEECVEVGVGSGRFAEPLGIKLGVEPSKRMAKIAES 76 Y+ W+E H Y +E N ++S ++C++VG G F E +G ++ V + + ES Sbjct: 13 YKRWYELHSKLYENERNVVRS-FNLKDCLDVGSGPDIFHEEIGGRI-VALDISLLMLKES 70 Query: 77 RGIKVIEGVAEDLPFPDNSLECILMVTTICFVDDPEKAIKEAYRVLKPNGHIIIGFIDRE 136 + + + A LPF DNS +C+ T+CF++D + I+E R+ K II FI R+ Sbjct: 71 KSDEKVLADALHLPFRDNSFKCVFSSVTVCFIEDVKGFIREMARITKERA--IICFIARD 128 Query: 137 SKIGREYEK-NKDKSVFYREANFFSTQEIVNLLERNGFKVEKIVQTLFRRLE-EVDSVEP 194 S G YE K +Y A F S +E ++ + K+ +I TL + E E+D V Sbjct: 129 SPWGEYYENLGKSGHKYYSHARFISRREFYVII-NDFMKISRIRSTLKKMSETEIDGV-- 185 Query: 195 VKEGFGEGSFVAIRARKM 212 GSF+ + A M Sbjct: 186 --YNDDSGSFICVEAIPM 201 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.318 0.139 0.391 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 111991561 Number of Sequences: 2977 Number of extensions: 4650214 Number of successful extensions: 14133 Number of sequences better than 1.0e-10: 10 Number of HSP's better than 0.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 3 Number of HSP's that attempted gapping in prelim test: 14112 Number of HSP's gapped (non-prelim): 10 length of query: 311 length of database: 189,106,746 effective HSP length: 57 effective length of query: 254 effective length of database: 154,992,987 effective search space: 39368218698 effective search space used: 39368218698 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.7 bits) S2: 165 (68.7 bits)