BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0740 (tgtA) DE:archaeosine tRNA-ribosyltransferase (tgtA)
(584 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||F75090 archaeosine trna-ribosyltransferase (tgta) PAB0740 -... 1165 0.0
pir||E71052 hypothetical protein PH1116 - Pyrococcus horikoshii ... 1055 0.0
sp|O26278|Y176_METTH HYPOTHETICAL PROTEIN MTH176 >gi|7482035|pir... 523 e-147
sp|Q57878|Y436_METJA HYPOTHETICAL PROTEIN MJ0436 >gi|2129231|pir... 442 e-123
emb|CAC12611.1| (AL445067) queuine tRNA-ribosyltransferase relat... 413 e-114
gi|11498196 archaeosine tRNA-ribosyltransferase (tgtA) [Archaeog... 399 e-110
gb|AAG20139.1| (AE005092) archaeosine tRNA-ribosyltransferase; T... 386 e-106
pir||C72505 hypothetical protein APE2017 - Aeropyrum pernix (str... 307 3e-82
gb|AAK40613.1| Queuine/archaeosine-tRNA -ribosyltransferase (tgt... 296 3e-79
gi|11498315 conserved hypothetical protein [Archaeoglobus fulgid... 124 3e-27
sp|Q9X1P7|TGT_THEMA QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANIN... 119 7e-26
pir||G82831 queuine tRNA-ribosyltransferase XF0223 [imported] - ... 116 6e-25
pir||D81167 queuine tRNA-ribosyltransferase NMB0719 [imported] -... 116 8e-25
gb|AAA27705.1| (L33777) tRNA guanine transglycosylase [Zymomonas... 115 1e-24
sp|P28720|TGT_ZYMMO QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANIN... 115 1e-24
pir||T46898 queuine tRNA-ribosyltransferase (EC 2.4.2.29) [valid... 115 1e-24
pir||A81939 probable queuine tRNA-ribosyltransferase (EC 2.4.2.2... 114 2e-24
pdb|1EFZ|A Chain A, Mutagenesis And Crystallographic Studies Of ... 114 3e-24
pir||C38530 queuine tRNA-ribosyltransferase (EC 2.4.2.29) [valid... 112 1e-23
sp|Q54177|TGT_SHIFL QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANIN... 112 1e-23
pdb|1WKD| Trna-Guanine Transglycosylase 112 1e-23
pdb|1WKE| Trna-Guanine Transglycosylase 112 1e-23
sp|P19675|TGT_ECOLI QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANIN... 111 2e-23
pdb|1WKF| Trna-Guanine Transglycosylase 111 2e-23
sp|O32053|TGT_BACSU QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANIN... 109 6e-23
dbj|BAB04947.1| (AP001511) tRNA-guanine transglycosylase [Bacill... 107 4e-22
pir||H82284 queuine tRNA-ribosyltransferase VC0741 [imported] - ... 106 6e-22
gb|AAG18970.1| (AE004998) Vng0421c [Halobacterium sp. NRC-1] 106 6e-22
sp|Q9ZCK8|TGT_RICPR QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANIN... 100 4e-20
pir||D75256 queuine tRNA ribosyltransferase - Deinococcus radiod... 100 5e-20
pir||H83169 queuine tRNA-ribosyltransferase PA3823 [imported] - ... 94 3e-18
sp|P44594|TGT_HAEIN QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANIN... 93 7e-18
sp|O67331|TGT_AQUAE QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANIN... 90 6e-17
sp|Q9ZMF4|TGT_HELPJ QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANIN... 89 1e-16
sp|O08314|TGT_HELPY QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANIN... 88 3e-16
sp|Q23623|TGT_CAEEL PUTATIVE QUEUINE TRNA-RIBOSYLTRANSFERASE (TR... 88 3e-16
sp|Q9VPY8|TGT_DROME PROBABLE QUEUINE TRNA-RIBOSYLTRANSFERASE (TR... 87 4e-16
sp|Q55983|TGT_SYNY3 QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANIN... 84 3e-15
emb|CAA72784.1| (Y12061) tRNA-guanine transglycosylase [Helicoba... 83 6e-15
gb|AAG20138.1| (AE005092) archaeosine tRNA-ribosyltransferase; T... 83 8e-15
sp|P57233|TGT_BUCAI QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANIN... 82 2e-14
pir||G81302 queuine tRNA-ribosyltransferase (EC 2.4.2.29) Cj1010... 75 2e-12
pir||F72505 hypothetical protein APE2020 - Aeropyrum pernix (str... 75 3e-12
prf||1819268A tRNA guanine transglycosylase [Zymomonas mobilis] 73 1e-11
gi|11230788 queuine tRNA-ribosyltransferase [Mus musculus] >gi|7... 73 1e-11
>pir||F75090 archaeosine trna-ribosyltransferase (tgta) PAB0740 - Pyrococcus
abyssi (strain Orsay) >gi|5458539|emb|CAB50027.1|
(AJ248286) archaeosine tRNA-ribosyltransferase (tgtA)
[Pyrococcus abyssi]
Length = 584
Score = 1165 bits (2981), Expect = 0.0
Identities = 584/584 (100%), Positives = 584/584 (100%)
Query: 1 MSRGDKMLKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLIVEPKELEKMGFDII 60
MSRGDKMLKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLIVEPKELEKMGFDII
Sbjct: 1 MSRGDKMLKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLIVEPKELEKMGFDII 60
Query: 61 ITNSYIIYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNREIVEFQHR 120
ITNSYIIYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNREIVEFQHR
Sbjct: 61 ITNSYIIYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNREIVEFQHR 120
Query: 121 IGVDIGTFLDIPTPPDAPKEKAMEDLKITLERAREAEEIKEIAMNAAIQGSTYTDLRRYA 180
IGVDIGTFLDIPTPPDAPKEKAMEDLKITLERAREAEEIKEIAMNAAIQGSTYTDLRRYA
Sbjct: 121 IGVDIGTFLDIPTPPDAPKEKAMEDLKITLERAREAEEIKEIAMNAAIQGSTYTDLRRYA 180
Query: 181 ARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRPDRPVHLFGAGHPMVFALA 240
ARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRPDRPVHLFGAGHPMVFALA
Sbjct: 181 ARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRPDRPVHLFGAGHPMVFALA 240
Query: 241 VAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEYFPCSCPVCSRYTPQELREMPKEER 300
VAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEYFPCSCPVCSRYTPQELREMPKEER
Sbjct: 241 VAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEYFPCSCPVCSRYTPQELREMPKEER 300
Query: 301 ARLLAIHNLWVIKEEIERIKQAIREGELWRLVDERARSHPKLYSAYKRLLDHYTFLEEFE 360
ARLLAIHNLWVIKEEIERIKQAIREGELWRLVDERARSHPKLYSAYKRLLDHYTFLEEFE
Sbjct: 301 ARLLAIHNLWVIKEEIERIKQAIREGELWRLVDERARSHPKLYSAYKRLLDHYTFLEEFE 360
Query: 361 PVTKKSAVFKISHESLRWPLVRRARERAERVNSKFGDLVEHPIFGKVTKYLTLTYPFAQS 420
PVTKKSAVFKISHESLRWPLVRRARERAERVNSKFGDLVEHPIFGKVTKYLTLTYPFAQS
Sbjct: 361 PVTKKSAVFKISHESLRWPLVRRARERAERVNSKFGDLVEHPIFGKVTKYLTLTYPFAQS 420
Query: 421 EAEDEFSIEKPTRENAIRYVMAIAEYQFGENASKAFEGAEVELARTGMPRQVKLNGKRLA 480
EAEDEFSIEKPTRENAIRYVMAIAEYQFGENASKAFEGAEVELARTGMPRQVKLNGKRLA
Sbjct: 421 EAEDEFSIEKPTRENAIRYVMAIAEYQFGENASKAFEGAEVELARTGMPRQVKLNGKRLA 480
Query: 481 TVRAEDGFLTLGIEGAKRLHKVLEYPRMRVVVSEEAEPFARKGKDVFAKFVLFADPGIRP 540
TVRAEDGFLTLGIEGAKRLHKVLEYPRMRVVVSEEAEPFARKGKDVFAKFVLFADPGIRP
Sbjct: 481 TVRAEDGFLTLGIEGAKRLHKVLEYPRMRVVVSEEAEPFARKGKDVFAKFVLFADPGIRP 540
Query: 541 YDEVLVVNEKDELLATGQALMSGREMIVFQYGRAVKVRRGISGG 584
YDEVLVVNEKDELLATGQALMSGREMIVFQYGRAVKVRRGISGG
Sbjct: 541 YDEVLVVNEKDELLATGQALMSGREMIVFQYGRAVKVRRGISGG 584
>pir||E71052 hypothetical protein PH1116 - Pyrococcus horikoshii
>gi|3257532|dbj|BAA30215.1| (AP000005) 582aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 582
Score = 1055 bits (2698), Expect = 0.0
Identities = 505/581 (86%), Positives = 566/581 (96%)
Query: 1 MSRGDKMLKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLIVEPKELEKMGFDII 60
MSRGDKMLKFE+KARDGAGRIGKLEVNGKKIETPAIMPVVNPKQ++VEPKELEKMGF+II
Sbjct: 1 MSRGDKMLKFEIKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQMVVEPKELEKMGFEII 60
Query: 61 ITNSYIIYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNREIVEFQHR 120
ITNSYIIYKD ELR KALE+GIH++L Y+GIIEVDSGSFQLM+YG+++VSNREI+EFQHR
Sbjct: 61 ITNSYIIYKDEELRRKALELGIHRMLDYNGIIEVDSGSFQLMKYGSIEVSNREIIEFQHR 120
Query: 121 IGVDIGTFLDIPTPPDAPKEKAMEDLKITLERAREAEEIKEIAMNAAIQGSTYTDLRRYA 180
IGVDIGTFLDIPTPPDAP+E+A+++L+ITL RAREAEEIKEI MNA IQGSTYTDLRRYA
Sbjct: 121 IGVDIGTFLDIPTPPDAPREQAVKELEITLSRAREAEEIKEIPMNATIQGSTYTDLRRYA 180
Query: 181 ARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRPDRPVHLFGAGHPMVFALA 240
ARRLSSMNFEIHPIGGVVPLLE+YRFR+VVDIVISSKMALRPDRPVHLFGAGHP+VFALA
Sbjct: 181 ARRLSSMNFEIHPIGGVVPLLESYRFRDVVDIVISSKMALRPDRPVHLFGAGHPIVFALA 240
Query: 241 VAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEYFPCSCPVCSRYTPQELREMPKEER 300
VAMGVDLFDSASYALYAKDDRY+TPEGTKRLDEL+YFPCSCPVCS+YTPQELREMPKEER
Sbjct: 241 VAMGVDLFDSASYALYAKDDRYMTPEGTKRLDELDYFPCSCPVCSKYTPQELREMPKEER 300
Query: 301 ARLLAIHNLWVIKEEIERIKQAIREGELWRLVDERARSHPKLYSAYKRLLDHYTFLEEFE 360
RLLA+HNLWVIKEEI+R+KQAI+EGELWRLVDERARSHPKLYSAYKRLL+HYTFLEEFE
Sbjct: 301 TRLLALHNLWVIKEEIKRVKQAIKEGELWRLVDERARSHPKLYSAYKRLLEHYTFLEEFE 360
Query: 361 PVTKKSAVFKISHESLRWPLVRRARERAERVNSKFGDLVEHPIFGKVTKYLTLTYPFAQS 420
P+TKKSA+FKIS+ESLRWP+VRRA+ERA+ +N +FG+LVEHPIFG+V++YL+LTYPFAQS
Sbjct: 361 PITKKSALFKISNESLRWPVVRRAKERAKSINERFGELVEHPIFGRVSRYLSLTYPFAQS 420
Query: 421 EAEDEFSIEKPTRENAIRYVMAIAEYQFGENASKAFEGAEVELARTGMPRQVKLNGKRLA 480
EAED+F IEKPT+E+AI+YVMAIAEYQFGE AS+AF+ A+VEL++TGMPRQVK+NGKRLA
Sbjct: 421 EAEDDFKIEKPTKEDAIKYVMAIAEYQFGEGASRAFDDAKVELSKTGMPRQVKVNGKRLA 480
Query: 481 TVRAEDGFLTLGIEGAKRLHKVLEYPRMRVVVSEEAEPFARKGKDVFAKFVLFADPGIRP 540
TVRA+DG LTLGIEGAKRLH+VL YPRMRVVV++EAEPFARKGKDVFAKFV+FADPGIRP
Sbjct: 481 TVRADDGLLTLGIEGAKRLHRVLPYPRMRVVVNKEAEPFARKGKDVFAKFVIFADPGIRP 540
Query: 541 YDEVLVVNEKDELLATGQALMSGREMIVFQYGRAVKVRRGI 581
YDEVLVVNE DELLATGQAL+SGREMIVFQYGRAVKVR+G+
Sbjct: 541 YDEVLVVNENDELLATGQALLSGREMIVFQYGRAVKVRKGV 581
>sp|O26278|Y176_METTH HYPOTHETICAL PROTEIN MTH176 >gi|7482035|pir||H69101 archaeosine
tRNA-ribosyltransferase (EC 2.4.2.-) tgtA -
Methanobacterium thermoautotrophicum (strain Delta H)
>gi|2621219|gb|AAB84682.1| (AE000805) tRNA-guanine
transglycosylase [Methanobacterium thermoautotrophicum]
Length = 665
Score = 523 bits (1332), Expect = e-147
Identities = 295/666 (44%), Positives = 398/666 (59%), Gaps = 93/666 (13%)
Query: 1 MSRGDKMLK-------FEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLIVEPKELE 53
+SRG+ +L FE+K++DG GR G L+ + TPA+MPV++P + ++ K
Sbjct: 5 ISRGNTLLMTLWEDFMFEIKSKDGLGRTGILKTEHGTVRTPALMPVIHPGKQTIDVKG-- 62
Query: 54 KMGFDIIITNSYIIYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNRE 113
G +I+ITN+YIIY++ ELRE+AL G+H+L+ +DG I DSGSFQL YG+++V N E
Sbjct: 63 -PGAEIVITNAYIIYRNPELRERALSDGVHRLIDFDGPIMTDSGSFQLSEYGDIEVENPE 121
Query: 114 IVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITLERAREAEEIKE-IAMNAAIQGST 172
I+ FQ IG DIGT LDIPTPP +A+E++++TLERARE+ E +E + +NA +QGST
Sbjct: 122 IIRFQDEIGTDIGTSLDIPTPPGVSHRRAIEEVEVTLERARESIEYRERMMLNAVVQGST 181
Query: 173 YTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRPDRPVHLFGAG 232
+ DLRRY A RL+ + E+HPIG VVPL+E+YR+RE+VD V+SS L P RP HL GAG
Sbjct: 182 HPDLRRYCASRLAELPVELHPIGAVVPLMESYRYRELVDAVLSSVSELPPSRPRHLMGAG 241
Query: 233 HPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEYFPCSCPVCSRYTPQEL 292
HPM+FALAV+MG DLFDSA+Y LYA+DDR L+ EGT +L+ L+ PCSC VC+ YTP EL
Sbjct: 242 HPMLFALAVSMGCDLFDSAAYILYAEDDRLLSTEGTYKLENLQEMPCSCSVCTDYTPSEL 301
Query: 293 REMPKEERARLLAIHNLWVIKEEIERIKQAIREGELWRLVDERARSHPKLYSAYKRLLDH 352
M +EER L+A HNL V EI +++QAI +G L LV+ER R+HP+L Y+R+ ++
Sbjct: 302 MGMDREERRNLIAEHNLHVSFAEIRKVRQAIHDGNLMELVEERCRAHPRLLEGYRRMSEY 361
Query: 353 YTFLEEFEPVTKKSAVFKISHESLRWPLVRRARERAERVNSKFGD--------------- 397
+E+FEP +K+SA F ESL V R +RV G+
Sbjct: 362 LDLIEKFEPRSKRSAFFYTGPESLGRVEVHR---HLKRVKEHLGERLALVAPSRRPYSSS 418
Query: 398 ----------------------LVEHPIFGKVTKYLTLTYPFAQSEAE------------ 423
+V+ P FG + L YP AQS+A
Sbjct: 419 LPARIGGFSSLRPQSGGPWRVVVVDLP-FGIIPLELDQVYPLAQSDAPGIMDLDGEEFLR 477
Query: 424 ------------------DEFSIEKP--------TRENAIRYVMAIAEYQFGENASKAFE 457
E IE P T + + V +A+YQFG A +
Sbjct: 478 GLVRDLMDGDAIVDDALCSELGIELPYKYMGEVETTVDDLDRVRMVADYQFGMGAGELLF 537
Query: 458 GAEVELAR---TGMPRQVKLNGKRLATVRAEDGFLTLGIEGAKRLHKVLEYPRMRVVVSE 514
+V + R TG R + + + T+RA DG L LG EGA RLHK +YP RV V+E
Sbjct: 538 TDDVRIERSRNTGKIRHIYAGDELICTMRASDGLLVLGAEGAVRLHKGTDYPAWRVAVNE 597
Query: 515 EAEPFARKGKDVFAKFVLFADPGIRPYDEVLVVNEKDELLATGQALMSGREMIVFQYGRA 574
E+EPFARKGK VFAKF++ D IR DEVL+VN DELLATG+AL+ EM+ +G+A
Sbjct: 598 ESEPFARKGKSVFAKFIIDCDNNIRANDEVLIVNADDELLATGKALLCAEEMMDLNHGQA 657
Query: 575 VKVRRG 580
VK R+G
Sbjct: 658 VKTRKG 663
>sp|Q57878|Y436_METJA HYPOTHETICAL PROTEIN MJ0436 >gi|2129231|pir||D64354 queuine
tRNA-ribosyltransferase (EC 2.4.2.29) - Methanococcus
jannaschii >gi|1591140|gb|AAB98424.1| (U67495) queuine
tRNA-ribosyltransferase (tgtA) [Methanococcus
jannaschii]
Length = 550
Score = 442 bits (1125), Expect = e-123
Identities = 250/545 (45%), Positives = 337/545 (60%), Gaps = 76/545 (13%)
Query: 113 EIVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITLERAREAEEIKE-----IAMNAA 167
EI+EFQ RIGVD+GT LDIPTPPD +E+A ++L+ TL+RA+ + E+KE + +N
Sbjct: 2 EIIEFQERIGVDVGTILDIPTPPDVDRERAEKELEETLKRAKASIELKEERGFKLLLNGT 61
Query: 168 IQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRPDRPVH 227
+QGSTY DLR+ +A+ ++ + F+I+PIG VVPL+E YR+R+V +I+I+SKM L ++PVH
Sbjct: 62 VQGSTYLDLRQKSAKEMAKLGFDIYPIGAVVPLMEQYRYRDVAEIIINSKMYLPTNKPVH 121
Query: 228 LFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDE---LEYFPCSCPVC 284
LFG GHPM FALAVA+G DLFDSA+YALYAKDDRYLT GT L+E L+ FPCSCPVC
Sbjct: 122 LFGCGHPMFFALAVALGCDLFDSAAYALYAKDDRYLTERGTLHLEEIKDLKAFPCSCPVC 181
Query: 285 SRYTPQELREMPKEERARLLAIHNLWVIKEEIERIKQAIREGELWRLVDERARSHPKLYS 344
S YTP+EL + K+ER RLLA HNL+V EEI RIKQAIR+G LW LV+ER R HPKL
Sbjct: 182 SSYTPKELASLNKKERERLLAEHNLYVTFEEINRIKQAIRDGSLWELVEERVRCHPKLLE 241
Query: 345 AYKRLLDHYTFLEEFEPVTKKSAVFKISHESLRWPLVRRARERAER-------------- 390
AY+ + + ++E+F+PVTKKSA F ES+ P V R ++R +R
Sbjct: 242 AYRVVRKYIDYIEKFDPVTKKSAFFYTGIESMFRPEVLRHKKRLKRLRYEKVYITTVSSS 301
Query: 391 -----------VNSKFGDLVEHPIFGKVTKYLTLTYPFAQSEAEDEFSIEKP-------- 431
V + L++ P+FG + Y+ YP +Q E + F EK
Sbjct: 302 IEKPYHEHLNVVETDVDILIKDPVFGFIPYYIDTVYPLSQHEIPELFDYEKEINKRFVDE 361
Query: 432 ------------------TRENAIRYVMA-------------IAEYQFGENASKAFEGAE 460
T I Y A + +YQ+G + +
Sbjct: 362 FIDWLKKKIGEDNILDIMTYNYYINYFSANKKINADALRIRKMLQYQYGFDIIDDELMNK 421
Query: 461 VELAR---TGMPRQV-KLNGKRLATVRAEDGFLTLGIEGAKRLHKVLEYPRMRVVVSEEA 516
+++ R TG RQV NG+ L +VR+ D L +GAK L K + +P+ RVVV++EA
Sbjct: 422 IKVVRSKTTGRLRQVLDENGEILFSVRSNDNLLIPSEKGAKLLWKKIPFPKYRVVVNKEA 481
Query: 517 EPFARKGKDVFAKFVLFADPGIRPYDEVLVVNEKDELLATGQALMSGREMIVFQYGRAVK 576
E FAR+G++VFAKFV+ D +RPY+EVLVVNE DELLA G +++G E+ F YG AVK
Sbjct: 482 EEFAREGRNVFAKFVIDCDEELRPYEEVLVVNEDDELLAYGTTILNGIELREFNYGLAVK 541
Query: 577 VRRGI 581
VR G+
Sbjct: 542 VRGGL 546
>emb|CAC12611.1| (AL445067) queuine tRNA-ribosyltransferase related protein
[Thermoplasma acidophilum]
Length = 632
Score = 413 bits (1049), Expect = e-114
Identities = 249/627 (39%), Positives = 350/627 (55%), Gaps = 59/627 (9%)
Query: 11 EVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLIVEPKELEKMGFDIIITNSYIIYKD 70
+++ RDG RI K E IETP ++PV+NP + + P+E++ +G IITNSYII +
Sbjct: 2 KIEERDGLARIAKFETPHGPIETPTVLPVINPNIMNITPQEMKPLGLQGIITNSYIILRT 61
Query: 71 RELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNREIVEFQHRIGVDIGTFLD 130
ELRE+AL G+H L+GYDG I DSG+FQ YG+++ +NRE+V+FQ RIG DI T LD
Sbjct: 62 PELRERALREGLHSLIGYDGPIMTDSGTFQSYVYGSIEFNNREVVDFQRRIGSDISTILD 121
Query: 131 IPTPPDAPKEKAMEDLKITLERAREAEEIKEIAMNAAIQGSTYTDLRRYAARRLSSMNFE 190
+ T P PK +A + + T R E + +E + +QG Y DLR+ +A ++S N
Sbjct: 122 VFTTPGTPKPEAEKAVIETYNRMLEVND-EEGIIAGPVQGGVYPDLRQKSAELMNSTNAG 180
Query: 191 IHPIGGVVPLLEAYRFREVVDIVISSKMALRPDRPVHLFGAGHPMVFALAVAMGVDLFDS 250
HPIGGVVPLLE Y + +VDI+I+SK+ L ++PVHLFG GHPM FA +V +GVDLFDS
Sbjct: 181 YHPIGGVVPLLETYDYSTLVDIIINSKINLSFNKPVHLFGGGHPMFFAFSVYLGVDLFDS 240
Query: 251 ASYALYAKDDRYLTPEGTKRLDELEYFPCSCPVCSRYTPQELREMPKEERARLLAIHNLW 310
ASY YAKDDR + P+GT+ L + P P+ RYT +E+R++ KE R+ +A HNL
Sbjct: 241 ASYIKYAKDDRLIYPDGTRDLARITELPQWSPLYDRYTVKEIRDLDKERRSLEIARHNLK 300
Query: 311 VIKEEIERIKQAIREGELWRLVDERARSHPKLYSAYKRLLDHYTFLEEFEPVTKKSAVFK 370
I EI IK+ I E L + V ++ARSHP L AY R++ + LE++E ++KK+A F
Sbjct: 301 AIFMEISEIKERIYEEGLAQYVAQKARSHPSLMKAYSRIMSYSNILEKYEDLSKKTAYFF 360
Query: 371 ISHESLRWPLVRRARERAER---------------------VNSKFGDLVEHPIFGK--- 406
S R P V R E NS+F V I
Sbjct: 361 YDSFSTRNPYVSRINRFTESYLSSNKKDTYAFTYRAWNPGYTNSEFVRDVYQKIDCNALI 420
Query: 407 ------VTKYLTLTYPFAQS-----EAEDEFSIEK-------------------PTRENA 436
V L TYP Q+ E + +FS K R
Sbjct: 421 SWSGTFVPAELENTYPIEQTVSSGFEPDPDFSRAKDLIAPFRVDMYKGEKFEGEQVRSFN 480
Query: 437 IRYVMAIAEYQFGENASKAF--EGAEVELARTGMPRQV-KLNGKRLATVRAEDGFLTLGI 493
+ + +A+YQFG + + + +++TG R + G+++AT+R DGF TL
Sbjct: 481 LNKIRMVADYQFGSGVGRMIFRDDVRINVSKTGRIRGILSKEGRQIATMR-NDGFFTLTY 539
Query: 494 EGAKRLHKVLEYPRMRVVVSEEAEPFARKGKDVFAKFVLFADPGIRPYDEVLVVNEKDEL 553
GA +H L+ P MRV VS+E+ + KG VF KF+L +D I ++VLVV+E D L
Sbjct: 540 YGASLIHSQLKPPAMRVTVSKESAEYNAKGYSVFFKFILGSDENIIAKNDVLVVDEDDVL 599
Query: 554 LATGQALMSGREMIVFQYGRAVKVRRG 580
A G+A++SGRE+ + G AVKV G
Sbjct: 600 AAVGKAMVSGRELREYTEGIAVKVHEG 626
>gi|11498196 archaeosine tRNA-ribosyltransferase (tgtA) [Archaeoglobus fulgidus]
>gi|3123066|sp|O29667|Y588_ARCFU HYPOTHETICAL PROTEIN
AF0588 >gi|7482908|pir||D69323 archaeosine
tRNA-ribosyltransferase (EC 2.4.2.-) tgtA -
Archaeoglobus fulgidus >gi|2650035|gb|AAB90652.1|
(AE001064) archaeosine tRNA-ribosyltransferase (tgtA)
[Archaeoglobus fulgidus]
Length = 481
Score = 399 bits (1015), Expect = e-110
Identities = 212/451 (47%), Positives = 297/451 (65%), Gaps = 18/451 (3%)
Query: 7 MLKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLIVEPKELEKMGFDIIITNSYI 66
M +FE+ +D GRI ++E +IETP I+PV+NP + +E++K G +ITNSYI
Sbjct: 1 MQRFEILDKDAMGRICRIETPHGRIETPTILPVINPNIPFIRAEEMKKFGAQAVITNSYI 60
Query: 67 IYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNREIVEFQHRIGVDIG 126
IY R +RE+ALE G+H +L D + DSGS+QLM YG+V++ N EIVEFQ IG DI
Sbjct: 61 IY--RSMREEALEKGVHGILETDMPVMTDSGSYQLMVYGDVEIKNAEIVEFQRHIGSDII 118
Query: 127 TFLDIPTPPDAPKEKAMEDLKITLERAREAEEIKEIAMN---AAIQGSTYTDLRRYAARR 183
LDIPTPPDA A DL+ITLER REA+E+ + A N +QGST+ DLRR+AA
Sbjct: 119 VPLDIPTPPDADYATAESDLRITLEREREAKELLKGAENLLAVPVQGSTHPDLRRFAAGE 178
Query: 184 LSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRPDRPVHLFGAGHPMVFALAVAM 243
+ +I+PIG VVPL++AYRFR++ +++ + AL P P+HLFG GHPM+FA+AVA+
Sbjct: 179 ARKIGGDIYPIGAVVPLMDAYRFRDLARVILEVRSAL-PVEPIHLFGCGHPMLFAMAVAL 237
Query: 244 GVDLFDSASYALYAKDDRYLTPEGTKRLDELEYFPCSCPVCSRYTPQELREMPKEERARL 303
G DLFDSA+YALYAKDDRYLT GTK+L EL YFPC CPVCS + P+ELR M K ER RL
Sbjct: 238 GCDLFDSAAYALYAKDDRYLTVYGTKKLSELNYFPCKCPVCSNHDPEELRRMEKNERERL 297
Query: 304 LAIHNLWVIKEEIERIKQAIREGELWRLVDERARSHPKLYSAYKRLLDHYTFLEEFEPVT 363
+A HNL+V +EIE IKQAI+E L+ LV++R R+HP + + ++++ ++ LE+ +P
Sbjct: 298 IAEHNLYVSFQEIETIKQAIKENSLFELVEKRVRAHPNMLAGWRQVKHYWELLEKADPKM 357
Query: 364 KKSAVFKISHESLRWPLVRRARERAER--------VNSKFG---DLVEHPIFGKVTKYLT 412
K+ ++ +SL P VRR + + V++ FG ++ P+FG V +
Sbjct: 358 KRKFLY-TGIDSLYRPAVRRHVKAIKNVELPEEVLVSTDFGIYANIYLRPVFGPVPAEML 416
Query: 413 LTYPFAQSEAEDEFSIEKPTRENAIRYVMAI 443
TYP +E +E +E+ + A +M +
Sbjct: 417 ETYPAGHAEIPEEDVVEEEALKAASEALMEL 447
>gb|AAG20139.1| (AE005092) archaeosine tRNA-ribosyltransferase; TgtA1
[Halobacterium sp. NRC-1]
Length = 491
Score = 386 bits (980), Expect = e-106
Identities = 218/488 (44%), Positives = 294/488 (59%), Gaps = 31/488 (6%)
Query: 10 FEVKARDGAGRIGKLEVN--GKKIETPAIMPVVNPKQLIVEPKE-LEKMGFDIIITNSYI 66
FEV A+DG RIG+L+V G +ETP +MPVVNP + VEP +++ G +++ITNSYI
Sbjct: 5 FEVGAQDGLARIGELDVPRAGVTVETPTLMPVVNPNLITVEPSRFVDEFGAELLITNSYI 64
Query: 67 IYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNREIVEFQHRIGVDIG 126
I D +LRE+AL+ G+H++LG+DG I DSGSFQL YG +D I+ FQH IG DIG
Sbjct: 65 INSDDDLRERALDEGLHEMLGFDGAIMTDSGSFQLAEYGEIDTDTEAILRFQHDIGSDIG 124
Query: 127 TFLDIPTPPDAPKEKAMEDLKITLERAREAE--EIKEIAMNAAIQGSTYTDLRRYAARRL 184
T +DIPTPPDA +E+A +L+ T +R AE ++ ++ +NA +QG+T DLR A
Sbjct: 125 TPVDIPTPPDADREQAAAELETTQQRLELAETVDVGDMLVNAPVQGATQPDLREQAGAHA 184
Query: 185 SSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRPDRPVHLFGAGHPMVFALAVAMG 244
++ P+G VVPL+ YR+ ++ + V+++K L D PVHLFGAGHPM+FALA A+G
Sbjct: 185 YGTALDLFPVGAVVPLMNQYRYDDMTEAVLAAKRGLGRDAPVHLFGAGHPMMFALAAALG 244
Query: 245 VDLFDSASYALYAKDDRYLTPEGTKRLDELEYFPCSCPVCSRYTPQELREMPKEERARLL 304
DLFDSA+YA+YA+DDRYLT GT+ LD+L YFPC CPVC+ ++PQ +R M +R RLL
Sbjct: 245 CDLFDSAAYAIYARDDRYLTVSGTEHLDDLHYFPCDCPVCAEHSPQAVRGMAAGDRERLL 304
Query: 305 AIHNLWVIKEEIERIKQAIREGELWRLVDERARSHPKLYSAYKRLLDHYTFLEEFEPVTK 364
A HNL V EI R+KQAI+ G L LV RA +HP Y+ LLDH LE +P +
Sbjct: 305 AEHNLHVSFGEIRRVKQAIKSGTLMELVAARAHAHPSTLDGYRALLDHSDQLEASDPAS- 363
Query: 365 KSAVFKISHESLRWPLVRRARERAERVNSKFGDLV------EH---------PIFGKVTK 409
K A F S R P V R +R +R++ D++ H P FG
Sbjct: 364 KDAFFYTGAGSARRPEVHRHHQRLDRLDVDGDDVLLTEGDSNHRYDESWNVLPPFGPYPS 423
Query: 410 YLTLTYPFAQSEAEDEFSIEKPTRENAIRYVMAIAEYQFGENASKAFEGAE---VELART 466
L TYP AE +++ E A V +AE N AF A E A
Sbjct: 424 ALATTYPLT---AETPARMDRAGYEAAAEGVCRLAE----ANPDTAFTLAHDDWPESALE 476
Query: 467 GMPRQVKL 474
+P++V L
Sbjct: 477 AVPQRVSL 484
>pir||C72505 hypothetical protein APE2017 - Aeropyrum pernix (strain K1)
>gi|5105715|dbj|BAA81027.1| (AP000063) 512aa long
hypothetical protein [Aeropyrum pernix]
Length = 512
Score = 307 bits (777), Expect = 3e-82
Identities = 160/353 (45%), Positives = 224/353 (63%), Gaps = 7/353 (1%)
Query: 10 FEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLIVEPKELEKMGFDIIITNSYIIYK 69
FE++ D AGRIG++ +ETPA PV++ + V E+ GF +ITN+Y+++K
Sbjct: 2 FEIRDVDLAGRIGRIYTQHGVVETPAFFPVIDVYRQEVSVDEVRAAGFGQVITNAYLLWK 61
Query: 70 DRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNREIVEFQHRIGVDIGTFL 129
+A E G+H++LG+ G++ DSG++Q++ YG V++S E+VE+Q R+G DI L
Sbjct: 62 --RFGWEAAEKGVHRILGFPGVVMTDSGAYQILEYGGVELSQGEVVEYQKRLGSDIAVIL 119
Query: 130 DIPTPPDAPKEKAMEDLKITLERAREAE---EIKEIAMNAAIQGSTYTDLRRYAARRLSS 186
DIPT D + +A E ++ T+ RA EA E E +QG Y DL +AR
Sbjct: 120 DIPTG-DVGRREAEESVRETIRRALEARVMIEGDERIWVYPVQGGRYFDLVEESARVGGR 178
Query: 187 MNF-EIHPIGGVVPLLEAYRFREVVDIVISSKMALRPDRPVHLFGAGHPMVFALAVAMGV 245
+ F ++ IG LE Y + VV+ V +K L RPVHLFGAGHP++F AVA+GV
Sbjct: 179 LGFYRMYGIGSPTVFLERYMYHVVVEAVYRAKKHLPWGRPVHLFGAGHPLIFPYAVALGV 238
Query: 246 DLFDSASYALYAKDDRYLTPEGTKRLDELEYFPCSCPVCSRYTPQELREMPKEERARLLA 305
D FDSASY LYA++ RY+T G R+++L+Y PCSCPVCSRYTPQELREM + ER RLLA
Sbjct: 239 DTFDSASYILYAREGRYITEYGVYRIEDLDYLPCSCPVCSRYTPQELREMDRVERTRLLA 298
Query: 306 IHNLWVIKEEIERIKQAIREGELWRLVDERARSHPKLYSAYKRLLDHYTFLEE 358
+HNL+VI + R+KQAIREG LW L++E +R HP R+ + LE+
Sbjct: 299 LHNLYVISASMRRVKQAIREGRLWELLEETSRKHPSTARVMARMRRYIDALEK 351
>gb|AAK40613.1| Queuine/archaeosine-tRNA -ribosyltransferase (tgtA) [Sulfolobus
solfataricus]
Length = 503
Score = 296 bits (751), Expect = 3e-79
Identities = 176/464 (37%), Positives = 259/464 (54%), Gaps = 50/464 (10%)
Query: 7 MLKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNP-KQLIVEPKELEKMGFDIIITNSY 65
M FEVK D AGRIG L +ETPA PV+N K+ + E+ +GF ITNSY
Sbjct: 1 MTVFEVKYEDLAGRIGTLRTRSGTLETPAFFPVINVLKKDEISIDEIRNIGFKNFITNSY 60
Query: 66 IIYKDRELREKALEVGIHK-LLGYDGIIEVDSGSFQLMRYGNVDVSNREIVEFQHRIGVD 124
I+YK+ +++ IHK L + II DSG++Q++ YG + ++N +IV +Q +I D
Sbjct: 61 ILYKNNYIKDD-----IHKELRSEEMIIMTDSGAYQILEYGEIGITNLQIVNYQLKIKPD 115
Query: 125 IGTFLDIPTPPDAPKEKAMEDLKITLERAREAEEI------KEIAMNAAIQGSTYTDLRR 178
IG LD+PT + A + + TL+RA EA EI I IQG Y DL +
Sbjct: 116 IGVILDLPTGNINDYDNAKKTVYETLKRAEEASEIIVKNQDNNIIWVYPIQGGRYLDLVK 175
Query: 179 YAARRLSSMN--FEIHPIGGVVPLLEAYRFREVVDIVISSKMALRPDRPVHLFGAGHPMV 236
+A LS + + +G LLE Y + V+D++ ++K ++ P HLFG G P +
Sbjct: 176 TSAEGLSKFEHIYNMAALGSPTVLLEKYMYDTVIDMIYTAKSNIKRGIPFHLFGGGLPHI 235
Query: 237 FALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEYFPCSCPVCSRYTPQELREMP 296
AVA+GVD FDSASY +YA+D+RY+T +L++LEYFPCSCP+CS+YTP++L EM
Sbjct: 236 IPFAVALGVDSFDSASYIIYARDNRYITRTRVYKLEDLEYFPCSCPICSKYTPKDLLEMN 295
Query: 297 KEERARLLAIHNLWVIKEEIERIKQAIREGELWRLVDERARSHPKLYSAYKRLLDHYTFL 356
++ER + LAIHNL+ I EE + KQAI+EG L+ + E+A SHP +YSA+KRL+ + +L
Sbjct: 296 EKERTKALAIHNLYTILEEFKATKQAIKEGRLFEYLQEKAYSHPAVYSAFKRLMKYKDYL 355
Query: 357 EEFEPVTK--KSAVFKISHESLRWP-LVRRAR--------------------------ER 387
E+F+P + +F SL P ++R +R +
Sbjct: 356 EKFDPRIRGDPKGLFLFDGNSLHRPEIIRHSRFLERYIQKKDKISIYCYDKAISDTAYDF 415
Query: 388 AERVNSKFGDLVEH------PIFGKVTKYLTLTYPFAQSEAEDE 425
E++ K D E P FG + ++ +YP +Q E +E
Sbjct: 416 KEKIREKIADRNESDVFIAVPFFGLIPLEISDSYPLSQFEIPNE 459
>gi|11498315 conserved hypothetical protein [Archaeoglobus fulgidus]
>gi|7483045|pir||D69338 conserved hypothetical protein
AF0708 - Archaeoglobus fulgidus
>gi|2649908|gb|AAB90533.1| (AE001056) conserved
hypothetical protein [Archaeoglobus fulgidus]
Length = 154
Score = 124 bits (308), Expect = 3e-27
Identities = 66/147 (44%), Positives = 95/147 (63%), Gaps = 2/147 (1%)
Query: 437 IRYVMAIAEYQFGENASKAF--EGAEVELARTGMPRQVKLNGKRLATVRAEDGFLTLGIE 494
+R + IA+YQFG + + + L+ +G RQV G R+AT++ + G+ TL IE
Sbjct: 6 LRMLRVIADYQFGRGVGRVLFPDTCKFILSSSGRVRQVVDGGVRIATLKPDSGWFTLSIE 65
Query: 495 GAKRLHKVLEYPRMRVVVSEEAEPFARKGKDVFAKFVLFADPGIRPYDEVLVVNEKDELL 554
GA+R+ + L +P+MRVVV +E F GK VFAK V+ D IR DEV+VVN D+LL
Sbjct: 66 GARRVKEALPFPKMRVVVMDEVSDFIAAGKSVFAKHVVEVDEEIRANDEVVVVNTADKLL 125
Query: 555 ATGQALMSGREMIVFQYGRAVKVRRGI 581
ATG+A++S EM+ + G AVKVR+G+
Sbjct: 126 ATGRAVLSAFEMLEMERGMAVKVRQGV 152
>sp|Q9X1P7|TGT_THEMA QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANINE TRANSGLYCOSYLASE)
(GUANINE INSERTION ENZYME) >gi|7448764|pir||D72240 tRNA
guanine transglycosylase - Thermotoga maritima (strain
MSB8) >gi|4982128|gb|AAD36627.1|AE001801_14 (AE001801)
tRNA guanine transglycosylase [Thermotoga maritima]
Length = 369
Score = 119 bits (296), Expect = 7e-26
Identities = 94/352 (26%), Positives = 159/352 (44%), Gaps = 36/352 (10%)
Query: 8 LKFEVKARDGAGRIGKLEVNGKKIETPAIMPV-VNPKQLIVEPKELEKMGFDIIITNSYI 66
++FEVK G R+G ++++ +ETP MPV N ++ P++LE+ G +II++N++
Sbjct: 1 MEFEVKKTFGKARLGVMKLHHGAVETPVFMPVGTNASVKLLTPRDLEEAGAEIILSNTFH 60
Query: 67 IYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNREIV----------- 115
+ + L G+H +G+ I DSG FQ+ + + + +V
Sbjct: 61 LMLKPGVEIIKLHRGLHNFMGWKRPILTDSGGFQVFSLPKIRIDDEGVVFRSPIDGSKVF 120
Query: 116 -------EFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITLE---RAREAEEIKEIAMN 165
E Q +G DI D PDA E+ E + T R+++A + + A+
Sbjct: 121 LNPEISMEVQIALGSDICMVFDHCPVPDADYEEVKEATERTYRWALRSKKAFKTENQALF 180
Query: 166 AAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRPDRP 225
+QG Y DLRR +A +L+S+ F+ + IGG+ E E+ ++ + L D+P
Sbjct: 181 GIVQGGIYPDLRRESALQLTSIGFDGYAIGGLSIGEERSLTLEMTEVTVE---FLPEDKP 237
Query: 226 VHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEG--------TKRLDELEYF 277
+ G G P + V GVD+FDS A+ LT G KR E
Sbjct: 238 RYFMGGGSPELILELVDRGVDMFDSVFPTRIARHGTALTWNGKLNLKASYNKRSLEPVDE 297
Query: 278 PCSCPVCSRYTPQELREMPKEERAR---LLAIHNLWVIKEEIERIKQAIREG 326
C C C +T + + LL IHN+ + ++ ++++I G
Sbjct: 298 RCGCYTCKNFTRSYIHHLFDRGEVLGQILLTIHNINFMISLMKEVRRSIESG 349
>pir||G82831 queuine tRNA-ribosyltransferase XF0223 [imported] - Xylella
fastidiosa (strain 9a5c)
>gi|9105032|gb|AAF83036.1|AE003876_1 (AE003876) queuine
tRNA-ribosyltransferase [Xylella fastidiosa]
Length = 403
Score = 116 bits (288), Expect = 6e-25
Identities = 100/356 (28%), Positives = 169/356 (47%), Gaps = 40/356 (11%)
Query: 8 LKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLI-VEPKELEKMGFDIIITNSYI 66
L+F+++A DGA R G+L ++TPA MPV + V P +L +G +II+ N++
Sbjct: 23 LQFQLQATDGAARRGQLSFPCGTVQTPAFMPVGTYGAVKGVLPGQLCDLGAEIILGNTFH 82
Query: 67 IYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGN-------------------V 107
++ L A G+H + ++G I DSG FQ+ + V
Sbjct: 83 LFLRPGLEVIADHGGLHGFMRWNGPILTDSGGFQVFSLAHRRKISEQGVTFAAPTDGAQV 142
Query: 108 DVSNREIVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITL---ERAREAEEI--KEI 162
+ E ++ Q + DI D TP A ++ A + ++++L +R+R+A + +
Sbjct: 143 FLGPEESMKIQKVLNSDIVMIFDECTPYPATEDVARDSMELSLRWAQRSRDAHDALDNDA 202
Query: 163 AMNAAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRP 222
A+ IQG + DLR + L ++ F+ + IGG+ + E+ R V+ + ++
Sbjct: 203 ALFGIIQGGVHPDLRGRSLDGLQAIGFDGYGIGGLA-VGESESERNVILEYLHPRVPA-- 259
Query: 223 DRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEYF----- 277
DRP +L G G P +VA GVD+FD +A++ +Y T GT ++ Y
Sbjct: 260 DRPRYLMGVGRPEDLVESVARGVDMFDCVMPTRHARNGQYFTGFGTVKIRNACYARDVDP 319
Query: 278 ---PCSCPVC-SRYTPQELREMPK--EERARLL-AIHNLWVIKEEIERIKQAIREG 326
C CP C YT LR + + E A +L +HNLW + + ++ AI G
Sbjct: 320 IEPGCGCPACVGGYTRAYLRHLDRCNEMLASMLGTLHNLWYYETLMANMRAAITAG 375
>pir||D81167 queuine tRNA-ribosyltransferase NMB0719 [imported] - Neisseria
meningitidis (group B strain MD58)
>gi|7225945|gb|AAF41132.1| (AE002426) queuine
tRNA-ribosyltransferase [Neisseria meningitidis MC58]
Length = 371
Score = 116 bits (287), Expect = 8e-25
Identities = 102/372 (27%), Positives = 167/372 (44%), Gaps = 48/372 (12%)
Query: 7 MLKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQL-IVEPKELEKMGFDIIITNSY 65
MLKF + +DG R G LE+N KIETP MPV + + P+ L + II+ N+Y
Sbjct: 1 MLKFTLHKKDGLARRGTLELNHGKIETPVFMPVGTYGSVKAMNPQNLHDIKAQIILGNTY 60
Query: 66 IIYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNRE------------ 113
++ L G+H +G+D I DSG FQ+ ++ E
Sbjct: 61 HLWLRPGLEVIGQFGGLHGFIGWDKPILTDSGGFQVFSLSDMRKLTEEGCTFKSPINGDK 120
Query: 114 -------IVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITL---ERAREA-EEIKE- 161
++ Q + DI LD TP +A +E+A + L+++L ER+++A E++K
Sbjct: 121 LFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSLQMSLRWAERSKKAFEDLKNP 180
Query: 162 IAMNAAIQGSTYTDLRRYAARRLSSMNFEIHPIGGV---VPLLEAYRFREVVDIVISSKM 218
A+ +QG+ Y DLR + R L +F +GG+ P E YR V +
Sbjct: 181 NALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSVGEPKPEMYRMLRAVGPI----- 235
Query: 219 ALRPDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEY-- 276
L +P +L G G P VA G+D+FD A++ T G ++ ++
Sbjct: 236 -LPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNGWLFTRFGDLKIKNAKHKL 294
Query: 277 ------FPCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIREGE 327
C+C C ++ L + + A+L IHNL + + +++A+ +G
Sbjct: 295 DKRPIDESCTCYACQNFSRAYLHHLHRTGEILGAQLNTIHNLHFYQVIMAEMREAVEQG- 353
Query: 328 LWRLVDERARSH 339
+ D +AR H
Sbjct: 354 --KFADWQARFH 363
>gb|AAA27705.1| (L33777) tRNA guanine transglycosylase [Zymomonas mobilis]
Length = 395
Score = 115 bits (285), Expect = 1e-24
Identities = 97/368 (26%), Positives = 167/368 (45%), Gaps = 43/368 (11%)
Query: 10 FEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQL-IVEPKELEKMGFDIIITNSYIIY 68
F + AR+G R G +E+ I TPA MPV + ++P+ + G DII+ N+Y +
Sbjct: 25 FSIAAREGKARTGTIEMKRGVIRTPAFMPVGTAATVKALKPETVRATGADIILGNTYHLM 84
Query: 69 KDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNRE--------------- 113
A G+H +G+D I DSG +Q+M ++ + E
Sbjct: 85 LRPGAERIAKLGGLHSFMGWDRPILTDSGGYQVMSLSSLTKQSEEGVTFKSHLDGSRHML 144
Query: 114 ----IVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITL---ERAREA-EEIKEIAMN 165
+E QH +G DI D TP A +A ++ ++ +R+R+A + KE A N
Sbjct: 145 SPERSIEIQHLLGSDIVMAFDECTPYPATPSRAASSMERSMRWAKRSRDAFDSRKEQAEN 204
Query: 166 AAI----QGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALR 221
AA+ QGS + +LR+ +A L+ + F+ + +GG L E+ ++ S L
Sbjct: 205 AALFGIQQGSVFENLRQQSADALAEIGFDGYAVGG---LAVGEGQDEMFRVLDFSVPMLP 261
Query: 222 PDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEY----- 276
D+P +L G G P AV G+D+FD ++ + T +G + +
Sbjct: 262 DDKPHYLMGVGKPDDIVGAVERGIDMFDCVLPTRSGRNGQAFTWDGPINIRNARFSEDLK 321
Query: 277 ---FPCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIREGELWR 330
C C VC +++ + + + A L+ HN+ ++ +++I+ +I EG +
Sbjct: 322 PLDSECHCAVCQKWSRAYIHHLIRAGEILGAMLMTEHNIAFYQQLMQKIRDSISEGRFSQ 381
Query: 331 LV-DERAR 337
D RAR
Sbjct: 382 FAQDFRAR 389
>sp|P28720|TGT_ZYMMO QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANINE TRANSGLYCOSYLASE)
(GUANINE INSERTION ENZYME) >gi|9257091|pdb|1F3E|A Chain
A, A New Target For Shigellosis: Rational Design And
Crystallographic Studies Of Inhibitors Of Trna-Guanine
Transglycosylase >gi|2392507|pdb|1PUD| Trna-Guanine
Transglycosylase >gi|7767014|pdb|1ENU|A Chain A, A New
Target For Shigellosis: Rational Design And
Crystallographic Studies Of Inhibitors Of Trna-Guanine
Transglycosylase
Length = 386
Score = 115 bits (285), Expect = 1e-24
Identities = 97/368 (26%), Positives = 167/368 (45%), Gaps = 43/368 (11%)
Query: 10 FEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQL-IVEPKELEKMGFDIIITNSYIIY 68
F + AR+G R G +E+ I TPA MPV + ++P+ + G DII+ N+Y +
Sbjct: 16 FSIAAREGKARTGTIEMKRGVIRTPAFMPVGTAATVKALKPETVRATGADIILGNTYHLM 75
Query: 69 KDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNRE--------------- 113
A G+H +G+D I DSG +Q+M ++ + E
Sbjct: 76 LRPGAERIAKLGGLHSFMGWDRPILTDSGGYQVMSLSSLTKQSEEGVTFKSHLDGSRHML 135
Query: 114 ----IVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITL---ERAREA-EEIKEIAMN 165
+E QH +G DI D TP A +A ++ ++ +R+R+A + KE A N
Sbjct: 136 SPERSIEIQHLLGSDIVMAFDECTPYPATPSRAASSMERSMRWAKRSRDAFDSRKEQAEN 195
Query: 166 AAI----QGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALR 221
AA+ QGS + +LR+ +A L+ + F+ + +GG L E+ ++ S L
Sbjct: 196 AALFGIQQGSVFENLRQQSADALAEIGFDGYAVGG---LAVGEGQDEMFRVLDFSVPMLP 252
Query: 222 PDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEY----- 276
D+P +L G G P AV G+D+FD ++ + T +G + +
Sbjct: 253 DDKPHYLMGVGKPDDIVGAVERGIDMFDCVLPTRSGRNGQAFTWDGPINIRNARFSEDLK 312
Query: 277 ---FPCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIREGELWR 330
C C VC +++ + + + A L+ HN+ ++ +++I+ +I EG +
Sbjct: 313 PLDSECHCAVCQKWSRAYIHHLIRAGEILGAMLMTEHNIAFYQQLMQKIRDSISEGRFSQ 372
Query: 331 LV-DERAR 337
D RAR
Sbjct: 373 FAQDFRAR 380
>pir||T46898 queuine tRNA-ribosyltransferase (EC 2.4.2.29) [validated] -
Zymomonas mobilis >gi|498141|gb|AAA27704.1| (L33777)
tRNA guanine transglycosylase [Zymomonas mobilis]
>gi|11095422|gb|AAG29862.1|AF313764_5 (AF313764) tRNA
guanine transglycosylase [Zymomonas mobilis]
>gi|1583685|prf||2121335B tRNA guanine transglycosylase
[Zymomonas mobilis]
Length = 399
Score = 115 bits (285), Expect = 1e-24
Identities = 97/368 (26%), Positives = 167/368 (45%), Gaps = 43/368 (11%)
Query: 10 FEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQL-IVEPKELEKMGFDIIITNSYIIY 68
F + AR+G R G +E+ I TPA MPV + ++P+ + G DII+ N+Y +
Sbjct: 29 FSIAAREGKARTGTIEMKRGVIRTPAFMPVGTAATVKALKPETVRATGADIILGNTYHLM 88
Query: 69 KDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNRE--------------- 113
A G+H +G+D I DSG +Q+M ++ + E
Sbjct: 89 LRPGAERIAKLGGLHSFMGWDRPILTDSGGYQVMSLSSLTKQSEEGVTFKSHLDGSRHML 148
Query: 114 ----IVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITL---ERAREA-EEIKEIAMN 165
+E QH +G DI D TP A +A ++ ++ +R+R+A + KE A N
Sbjct: 149 SPERSIEIQHLLGSDIVMAFDECTPYPATPSRAASSMERSMRWAKRSRDAFDSRKEQAEN 208
Query: 166 AAI----QGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALR 221
AA+ QGS + +LR+ +A L+ + F+ + +GG L E+ ++ S L
Sbjct: 209 AALFGIQQGSVFENLRQQSADALAEIGFDGYAVGG---LAVGEGQDEMFRVLDFSVPMLP 265
Query: 222 PDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEY----- 276
D+P +L G G P AV G+D+FD ++ + T +G + +
Sbjct: 266 DDKPHYLMGVGKPDDIVGAVERGIDMFDCVLPTRSGRNGQAFTWDGPINIRNARFSEDLK 325
Query: 277 ---FPCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIREGELWR 330
C C VC +++ + + + A L+ HN+ ++ +++I+ +I EG +
Sbjct: 326 PLDSECHCAVCQKWSRAYIHHLIRAGEILGAMLMTEHNIAFYQQLMQKIRDSISEGRFSQ 385
Query: 331 LV-DERAR 337
D RAR
Sbjct: 386 FAQDFRAR 393
>pir||A81939 probable queuine tRNA-ribosyltransferase (EC 2.4.2.29) NMA0928
[imported] - Neisseria meningitidis (group A strain
Z2491) >gi|7379633|emb|CAB84200.1| (AL162754) putative
queuine tRNA-ribosyltransferase [Neisseria meningitidis
Z2491]
Length = 371
Score = 114 bits (283), Expect = 2e-24
Identities = 102/372 (27%), Positives = 168/372 (44%), Gaps = 48/372 (12%)
Query: 7 MLKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQL-IVEPKELEKMGFDIIITNSY 65
MLKF + +DG R G LE+N KIETP MPV + + P+ L + II+ N+Y
Sbjct: 1 MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNPQNLHDIKAQIILGNTY 60
Query: 66 IIYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNRE------------ 113
++ L G+H +G+D I DSG FQ+ ++ E
Sbjct: 61 HLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDMRKLTEEGCTFKSPINGDK 120
Query: 114 -------IVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITL---ERAREA-EEIKE- 161
++ Q + DI LD TP +A +E+A + L+++L ER+++A E++K
Sbjct: 121 LFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSLQMSLRWAERSKKAFEDLKNP 180
Query: 162 IAMNAAIQGSTYTDLRRYAARRLSSMNFEIHPIGGV---VPLLEAYRFREVVDIVISSKM 218
A+ +QG+ Y DLR + + L ++F IGG+ P E YR V +
Sbjct: 181 NALFGIVQGAMYEDLREESLKGLEELDFPGLAIGGLSVGEPKPEMYRMLRAVGPI----- 235
Query: 219 ALRPDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEY-- 276
L +P +L G G P VA GVD+FD A++ T G ++ ++
Sbjct: 236 -LPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNGWLFTRFGDLKIKNAKHKL 294
Query: 277 ------FPCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIREGE 327
C+C C ++ L + + A+L IHNL + + +++A+ +G
Sbjct: 295 DKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIHNLHFYQVIMAEMREAVEQG- 353
Query: 328 LWRLVDERARSH 339
+ D +A+ H
Sbjct: 354 --KFADWQAQFH 363
>pdb|1EFZ|A Chain A, Mutagenesis And Crystallographic Studies Of Zymomonas
Mobilis Trna-Guanine Transglycosylase To Elucidate The
Role Of Serine 103 For Enzymatic Activity
Length = 386
Score = 114 bits (282), Expect = 3e-24
Identities = 96/368 (26%), Positives = 167/368 (45%), Gaps = 43/368 (11%)
Query: 10 FEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQL-IVEPKELEKMGFDIIITNSYIIY 68
F + AR+G R G +E+ I TPA MPV + ++P+ + G DII+ N+Y +
Sbjct: 16 FSIAAREGKARTGTIEMKRGVIRTPAFMPVGTAATVKALKPETVRATGADIILGNTYHLM 75
Query: 69 KDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNRE--------------- 113
A G+H +G+D I D+G +Q+M ++ + E
Sbjct: 76 LRPGAERIAKLGGLHSFMGWDRPILTDAGGYQVMSLSSLTKQSEEGVTFKSHLDGSRHML 135
Query: 114 ----IVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITL---ERAREA-EEIKEIAMN 165
+E QH +G DI D TP A +A ++ ++ +R+R+A + KE A N
Sbjct: 136 SPERSIEIQHLLGSDIVMAFDECTPYPATPSRAASSMERSMRWAKRSRDAFDSRKEQAEN 195
Query: 166 AAI----QGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALR 221
AA+ QGS + +LR+ +A L+ + F+ + +GG L E+ ++ S L
Sbjct: 196 AALFGIQQGSVFENLRQQSADALAEIGFDGYAVGG---LAVGEGQDEMFRVLDFSVPMLP 252
Query: 222 PDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEY----- 276
D+P +L G G P AV G+D+FD ++ + T +G + +
Sbjct: 253 DDKPHYLMGVGKPDDIVGAVERGIDMFDCVLPTRSGRNGQAFTWDGPINIRNARFSEDLK 312
Query: 277 ---FPCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIREGELWR 330
C C VC +++ + + + A L+ HN+ ++ +++I+ +I EG +
Sbjct: 313 PLDSECHCAVCQKWSRAYIHHLIRAGEILGAMLMTEHNIAFYQQLMQKIRDSISEGRFSQ 372
Query: 331 LV-DERAR 337
D RAR
Sbjct: 373 FAQDFRAR 380
>pir||C38530 queuine tRNA-ribosyltransferase (EC 2.4.2.29) [validated] -
Escherichia coli >gi|1773090|gb|AAB40162.1| (U82664)
transfer RNA-guanine transglycosylase [Escherichia coli]
>gi|1786607|gb|AAC73509.1| (AE000147) tRNA-guanine
transglycosylase [Escherichia coli K12]
Length = 375
Score = 112 bits (278), Expect = 1e-23
Identities = 94/366 (25%), Positives = 158/366 (42%), Gaps = 45/366 (12%)
Query: 8 LKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLI-VEPKELEKMGFDIIITNSYI 66
+KFE+ DG R G+L + +ETP MPV + + P+E+E G II+ N++
Sbjct: 1 MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPEEVEATGAQIILGNTFH 60
Query: 67 IYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVD------------------ 108
++ L +H + + G I DSG FQ+ G++
Sbjct: 61 LWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPI 120
Query: 109 -VSNREIVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITLERAREAEEI-----KEI 162
+ + +E Q+ +G DI D TP A + A ++++L A+ + E +
Sbjct: 121 FLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRERFDSLGNKN 180
Query: 163 AMNAAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVV---PLLEAYRFREVVDIVISSKMA 219
A+ IQGS Y DLR + + L + F+ + +GG+ P + +R E V I +
Sbjct: 181 ALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPA--- 237
Query: 220 LRPDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEYF-- 277
D+P +L G G P V G+D+FD A++ +G ++ +Y
Sbjct: 238 ---DKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKYKSD 294
Query: 278 ------PCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIREGEL 328
C C C Y+ L + + ARL IHNL + + +++AI EG+L
Sbjct: 295 TGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKL 354
Query: 329 WRLVDE 334
V +
Sbjct: 355 ESFVTD 360
>sp|Q54177|TGT_SHIFL QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANINE TRANSGLYCOSYLASE)
(GUANINE INSERTION ENZYME) (VIRULENCE-ASSOCIATED PROTEIN
VACC) >gi|599588|dbj|BAA05482.1| (D26469) VacC [Shigella
flexneri]
Length = 375
Score = 112 bits (277), Expect = 1e-23
Identities = 94/366 (25%), Positives = 158/366 (42%), Gaps = 45/366 (12%)
Query: 8 LKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLI-VEPKELEKMGFDIIITNSYI 66
+KFE+ DG R G+L + +ETP MPV + + P+E+E G II+ N++
Sbjct: 1 MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPEEVEATGAQIILGNTFH 60
Query: 67 IYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVD------------------ 108
++ L +H + + G I DSG FQ+ G++
Sbjct: 61 LWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPI 120
Query: 109 -VSNREIVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITLERAREAEEI-----KEI 162
+ + +E Q+ +G DI D TP A + A ++++L A+ + E +
Sbjct: 121 FLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRERFDSLGNKN 180
Query: 163 AMNAAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVV---PLLEAYRFREVVDIVISSKMA 219
A+ IQGS Y DLR + + L + F+ + +GG+ P + +R E V I +
Sbjct: 181 ALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPA--- 237
Query: 220 LRPDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEYF-- 277
D+P +L G G P V G+D+FD A++ +G ++ +Y
Sbjct: 238 ---DKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKYKSD 294
Query: 278 ------PCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIREGEL 328
C C C Y+ L + + ARL IHNL + + +++AI EG+L
Sbjct: 295 TGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKL 354
Query: 329 WRLVDE 334
V +
Sbjct: 355 ESFVTD 360
>pdb|1WKD| Trna-Guanine Transglycosylase
Length = 386
Score = 112 bits (277), Expect = 1e-23
Identities = 96/368 (26%), Positives = 166/368 (45%), Gaps = 43/368 (11%)
Query: 10 FEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQL-IVEPKELEKMGFDIIITNSYIIY 68
F + AR+G R G +E+ I TPA MPV + ++P+ + G DII+ N+Y +
Sbjct: 16 FSIAAREGKARTGTIEMKRGVIRTPAFMPVGTAATVKALKPETVRATGADIILGNTYHLM 75
Query: 69 KDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNRE--------------- 113
A G+H +G+D I SG +Q+M ++ + E
Sbjct: 76 LRPGAERIAKLGGLHSFMGWDRPILTASGGYQVMSLSSLTKQSEEGVTFKSHLDGSRHML 135
Query: 114 ----IVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITL---ERAREA-EEIKEIAMN 165
+E QH +G DI D TP A +A ++ ++ +R+R+A + KE A N
Sbjct: 136 SPERSIEIQHLLGSDIVMAFDECTPYPATPSRAASSMERSMRWAKRSRDAFDSRKEQAEN 195
Query: 166 AAI----QGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALR 221
AA+ QGS + +LR+ +A L+ + F+ + +GG L E+ ++ S L
Sbjct: 196 AALFGIQQGSVFENLRQQSADALAEIGFDGYAVGG---LAVGEGQDEMFRVLDFSVPMLP 252
Query: 222 PDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEY----- 276
D+P +L G G P AV G+D+FD ++ + T +G + +
Sbjct: 253 DDKPHYLMGVGKPDDIVGAVERGIDMFDCVLPTRSGRNGQAFTWDGPINIRNARFSEDLK 312
Query: 277 ---FPCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIREGELWR 330
C C VC +++ + + + A L+ HN+ ++ +++I+ +I EG +
Sbjct: 313 PLDSECHCAVCQKWSRAYIHHLIRAGEILGAMLMTEHNIAFYQQLMQKIRDSISEGRFSQ 372
Query: 331 LV-DERAR 337
D RAR
Sbjct: 373 FAQDFRAR 380
>pdb|1WKE| Trna-Guanine Transglycosylase
Length = 386
Score = 112 bits (277), Expect = 1e-23
Identities = 96/368 (26%), Positives = 166/368 (45%), Gaps = 43/368 (11%)
Query: 10 FEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQL-IVEPKELEKMGFDIIITNSYIIY 68
F + AR+G R G +E+ I TPA MPV + ++P+ + G DII+ N+Y +
Sbjct: 16 FSIAAREGKARTGTIEMKRGVIRTPAFMPVGTAATVKALKPETVRATGADIILGNTYHLM 75
Query: 69 KDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNRE--------------- 113
A G+H +G+D I DSG +Q+M ++ + E
Sbjct: 76 LRPGAERIAKLGGLHSFMGWDRPILTDSGGYQVMSLSSLTKQSEEGVTFKSHLDGSRHML 135
Query: 114 ----IVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITL---ERAREA-EEIKEIAMN 165
+E QH +G DI TP A +A ++ ++ +R+R+A + KE A N
Sbjct: 136 SPERSIEIQHLLGSDIVMAFAECTPYPATPSRAASSMERSMRWAKRSRDAFDSRKEQAEN 195
Query: 166 AAI----QGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALR 221
AA+ QGS + +LR+ +A L+ + F+ + +GG L E+ ++ S L
Sbjct: 196 AALFGIQQGSVFENLRQQSADALAEIGFDGYAVGG---LAVGEGQDEMFRVLDFSVPMLP 252
Query: 222 PDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEY----- 276
D+P +L G G P AV G+D+FD ++ + T +G + +
Sbjct: 253 DDKPHYLMGVGKPDDIVGAVERGIDMFDCVLPTRSGRNGQAFTWDGPINIRNARFSEDLK 312
Query: 277 ---FPCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIREGELWR 330
C C VC +++ + + + A L+ HN+ ++ +++I+ +I EG +
Sbjct: 313 PLDSECHCAVCQKWSRAYIHHLIRAGEILGAMLMTEHNIAFYQQLMQKIRDSISEGRFSQ 372
Query: 331 LV-DERAR 337
D RAR
Sbjct: 373 FAQDFRAR 380
>sp|P19675|TGT_ECOLI QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANINE TRANSGLYCOSYLASE)
(GUANINE INSERTION ENZYME) >gi|147966|gb|AAA24667.1|
(M63939) transfer RNA-guanine transglycosylase
[Escherichia coli]
Length = 375
Score = 111 bits (276), Expect = 2e-23
Identities = 93/366 (25%), Positives = 158/366 (42%), Gaps = 45/366 (12%)
Query: 8 LKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLI-VEPKELEKMGFDIIITNSYI 66
+KFE+ DG R G+L + +ETP MPV + + P+E+E G I++ N++
Sbjct: 1 MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPEEVEATGAQIMVGNTFH 60
Query: 67 IYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVD------------------ 108
++ L +H + + G I DSG FQ+ G++
Sbjct: 61 LWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPI 120
Query: 109 -VSNREIVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITLERAREAEEI-----KEI 162
+ + +E Q+ +G DI D TP A + A ++++L A+ + E +
Sbjct: 121 FLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRERFDSLGNKN 180
Query: 163 AMNAAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVV---PLLEAYRFREVVDIVISSKMA 219
A+ IQGS Y DLR + + L + F+ + +GG+ P + +R E V I +
Sbjct: 181 ALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPA--- 237
Query: 220 LRPDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEYF-- 277
D+P +L G G P V G+D+FD A++ +G ++ +Y
Sbjct: 238 ---DKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKYKSD 294
Query: 278 ------PCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIREGEL 328
C C C Y+ L + + ARL IHNL + + +++AI EG+L
Sbjct: 295 TGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKL 354
Query: 329 WRLVDE 334
V +
Sbjct: 355 ESFVTD 360
>pdb|1WKF| Trna-Guanine Transglycosylase
Length = 386
Score = 111 bits (276), Expect = 2e-23
Identities = 96/368 (26%), Positives = 166/368 (45%), Gaps = 43/368 (11%)
Query: 10 FEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQL-IVEPKELEKMGFDIIITNSYIIY 68
F + AR+G R G +E+ I TPA MPV + ++P+ + G DII+ N+Y +
Sbjct: 16 FSIAAREGKARTGTIEMKRGVIRTPAFMPVGTAATVKALKPETVRATGADIILGNTYHLM 75
Query: 69 KDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNRE--------------- 113
A G+H +G+D I DSG +Q+M ++ + E
Sbjct: 76 LRPGAERIAKLGGLHSFMGWDRPILTDSGGYQVMSLSSLTKQSEEGVTFKSHLDGSRHML 135
Query: 114 ----IVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITL---ERAREA-EEIKEIAMN 165
+E QH +G DI TP A +A ++ ++ +R+R+A + KE A N
Sbjct: 136 SPERSIEIQHLLGSDIVMAFYECTPYPATPSRAASSMERSMRWAKRSRDAFDSRKEQAEN 195
Query: 166 AAI----QGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALR 221
AA+ QGS + +LR+ +A L+ + F+ + +GG L E+ ++ S L
Sbjct: 196 AALFGIQQGSVFENLRQQSADALAEIGFDGYAVGG---LAVGEGQDEMFRVLDFSVPMLP 252
Query: 222 PDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEY----- 276
D+P +L G G P AV G+D+FD ++ + T +G + +
Sbjct: 253 DDKPHYLMGVGKPDDIVGAVERGIDMFDCVLPTRSGRNGQAFTWDGPINIRNARFSEDLK 312
Query: 277 ---FPCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIREGELWR 330
C C VC +++ + + + A L+ HN+ ++ +++I+ +I EG +
Sbjct: 313 PLDSECHCAVCQKWSRAYIHHLIRAGEILGAMLMTEHNIAFYQQLMQKIRDSISEGRFSQ 372
Query: 331 LV-DERAR 337
D RAR
Sbjct: 373 FAQDFRAR 380
>sp|O32053|TGT_BACSU QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANINE TRANSGLYCOSYLASE)
(GUANINE INSERTION ENZYME) >gi|7448762|pir||B69722
queuine tRNA-ribosyltransferase (EC 2.4.2.29) - Bacillus
subtilis >gi|2635236|emb|CAB14731.1| (Z99118)
tRNA-guanine transglycosylase [Bacillus subtilis]
>gi|6977808|emb|CAB75333.1| (Y15896) tRNA-guanine
transglycosylase [Bacillus subtilis]
Length = 381
Score = 109 bits (271), Expect = 6e-23
Identities = 99/357 (27%), Positives = 160/357 (44%), Gaps = 42/357 (11%)
Query: 9 KFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQL-IVEPKELEKMGFDIIITNSYII 67
+F + + R+GK+ ETP MPV + + P+EL+ M II++N+Y +
Sbjct: 9 EFIKECKQTGARLGKVHTPHGSFETPVFMPVGTLATVKTMSPEELKAMDAGIILSNTYHL 68
Query: 68 YKDRELREKALEVG-IHKLLGYDGIIEVDSGSFQ---LMRYGNVD--------------- 108
+ R ++ E G +HK + +D I DSG FQ L ++ N++
Sbjct: 69 WL-RPGQDIVKEAGGLHKFMNWDRAILTDSGGFQVFSLSKFRNIEEEGVHFRNHLNGDKL 127
Query: 109 -VSNREIVEFQHRIGVDIGTFLDIPTPPDAPKE---KAMEDLKITLERAREAEEIK-EIA 163
+S + +E Q+ +G DI D P A + +++E ER A + E
Sbjct: 128 FLSPEKAMEIQNALGSDIMMAFDECPPYPAEYDYMKRSVERTSRWAERCLNAHNRQDEQG 187
Query: 164 MNAAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRP- 222
+ +QG Y DLR +A+ L S++F + IGG L ++V++ V+ L P
Sbjct: 188 LFGIVQGGEYEDLRTQSAKDLISLDFPGYAIGG----LSVGEPKDVMNRVLEFTTPLLPK 243
Query: 223 DRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLD----ELEYFP 278
D+P +L G G P GVD+FD A++ T EG + E ++ P
Sbjct: 244 DKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTVFTAEGRLNMKNAKFERDFRP 303
Query: 279 ----CSCPVCSRYTPQELREMPKEERA---RLLAIHNLWVIKEEIERIKQAIREGEL 328
C C C YT +R + + RL HNL + +E+++QAIRE L
Sbjct: 304 IDEECDCYTCKNYTRAYIRHLIRCNETFGLRLTTYHNLHFLLHLMEQVRQAIREDRL 360
>dbj|BAB04947.1| (AP001511) tRNA-guanine transglycosylase [Bacillus halodurans]
Length = 379
Score = 107 bits (264), Expect = 4e-22
Identities = 95/351 (27%), Positives = 158/351 (44%), Gaps = 42/351 (11%)
Query: 15 RDGAGRIGKLEVNGKKIETPAIMPVVNPKQL-IVEPKELEKMGFDIIITNSYIIYKDREL 73
+ R+GKL IETP MPV + + P+EL+++G II++N+Y ++ R
Sbjct: 13 KQSGARLGKLHTPHGTIETPIFMPVGTLATVKTMSPEELKQLGAQIILSNTYHLWL-RPG 71
Query: 74 REKALEVG-IHKLLGYDGIIEVDSGSFQLMRYGNVDVSNREIVEF--------------- 117
+ E G +H+ + +D I DSG FQ+ ++ E V F
Sbjct: 72 HDIVKEAGGLHEFMNWDRPILTDSGGFQVFSLSDLRTIEEEGVHFRNHLSGEKLFLSPEG 131
Query: 118 ----QHRIGVDIGTFLDIPTPPDAPKEKAMEDLKIT---LERAREAEEIKEI-AMNAAIQ 169
Q+ +G DI D P A ++ ++ T ER +A + E A+ IQ
Sbjct: 132 AMEIQNALGSDIMMAFDECPPYPAERDYMRPSVERTSRWAERCLKAHKRPEDQALFGIIQ 191
Query: 170 GSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRP-DRPVHL 228
G Y DLRR +A+ ++S++F + IGGV ++V++ V+ L P ++P +L
Sbjct: 192 GGEYEDLRRQSAQDITSLDFPGYAIGGV----SVGEPKDVMNRVLEFTTPLLPANKPRYL 247
Query: 229 FGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEYF--------PCS 280
G G P G+D+FD A++ +T G + +Y C
Sbjct: 248 MGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSNGRLVVRNAKYARDFRSLDENCD 307
Query: 281 CPVCSRYTPQELREMPKEERA---RLLAIHNLWVIKEEIERIKQAIREGEL 328
C VC YT +R + K + RL HNL+ + + ++ ++QAI + L
Sbjct: 308 CHVCQTYTRAYIRHLVKCDETFGFRLTTYHNLYFLLKLMKDVRQAILDDRL 358
>pir||H82284 queuine tRNA-ribosyltransferase VC0741 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9655186|gb|AAF93906.1|
(AE004160) queuine tRNA-ribosyltransferase [Vibrio
cholerae]
Length = 379
Score = 106 bits (263), Expect = 6e-22
Identities = 92/366 (25%), Positives = 157/366 (42%), Gaps = 45/366 (12%)
Query: 8 LKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLI-VEPKELEKMGFDIIITNSYI 66
LKFE+K ++G R G+L ++TPA MPV + + P+E+++ G I++ N++
Sbjct: 3 LKFELKKKNGNARRGQLIFERGTVQTPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFH 62
Query: 67 IYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNREIVEF--------- 117
++ + +H + + G I DSG FQ+ G++ E V F
Sbjct: 63 LWLRPGQEVMKMHGDLHDFMNWQGPILTDSGGFQVFSLGDIRKITEEGVHFRNPVNGDKI 122
Query: 118 ----------QHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITLERAREAEEIKEIAMN-- 165
Q +G DI D TP A ++A + ++++L A+ + + + N
Sbjct: 123 FMDAEKSMEIQKDLGSDIVMIFDECTPYPATHDEAKKSMEMSLRWAKRSRDHFDKLENPN 182
Query: 166 ---AAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVV---PLLEAYRFREVVDIVISSKMA 219
+QG Y DLR + + L+ + F+ + +GG+ P + +R E +
Sbjct: 183 NLFGIVQGGVYEDLRDVSVKGLTEIGFDGYAVGGLAVGEPKEDMHRVLE------HTCPQ 236
Query: 220 LRPDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRL-------D 272
L D+P +L G G P V G+D+FD A++ G ++ D
Sbjct: 237 LPEDKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVIKIRNAAHKTD 296
Query: 273 ELEYFP-CSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIREGEL 328
P C C C Y+ L + + ARL IHNL + +E I++AI E
Sbjct: 297 TTPLDPHCDCYTCKNYSKSYLHHLDRCNEILGARLNTIHNLRYYQRLMESIRKAIDEDRF 356
Query: 329 WRLVDE 334
+ V E
Sbjct: 357 DQFVAE 362
>gb|AAG18970.1| (AE004998) Vng0421c [Halobacterium sp. NRC-1]
Length = 159
Score = 106 bits (263), Expect = 6e-22
Identities = 66/154 (42%), Positives = 91/154 (58%), Gaps = 6/154 (3%)
Query: 433 RENAIRYVMAIAEYQFGENASKAF----EGAEVELARTGMPRQV-KLNGKRLATVRAEDG 487
++ I + +A YQ+G A +A + V+ + TG P+Q+ + +G RL +V DG
Sbjct: 2 QDAGIPALRTVAAYQYGAAAGRALFPEDKPHHVQRSSTGRPQQIHRDDGARLVSVGL-DG 60
Query: 488 FLTLGIEGAKRLHKVLEYPRMRVVVSEEAEPFARKGKDVFAKFVLFADPGIRPYDEVLVV 547
LTLG G +RL LE+PR RVVV +E+EPF R GK+VFAKFV D R DE+ VV
Sbjct: 61 RLTLGPAGGQRLIDALEHPRARVVVGDESEPFVRDGKNVFAKFVTDVDDDARQGDELAVV 120
Query: 548 NEKDELLATGQALMSGREMIVFQYGRAVKVRRGI 581
+E LLA G+A + M F+ G AV VR G+
Sbjct: 121 HEDGRLLAVGRAELDAASMRDFETGMAVSVRAGV 154
>sp|Q9ZCK8|TGT_RICPR QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANINE TRANSGLYCOSYLASE)
(GUANINE INSERTION ENZYME) >gi|7448761|pir||H71631
queuine tRNA-ribosyltransferase (tgt) RP721 - Rickettsia
prowazekii >gi|3861253|emb|CAA15152.1| (AJ235273)
QUEUINE TRNA-RIBOSYLTRANSFERASE (tgt) [Rickettsia
prowazekii]
Length = 361
Score = 100 bits (247), Expect = 4e-20
Identities = 95/355 (26%), Positives = 147/355 (40%), Gaps = 42/355 (11%)
Query: 10 FEVKARDGAGRIGKLEVNGKKIETPAIMPVVNP---KQLIVEPKELEKMGFDIIITNSYI 66
F + + R G + +I TPA MPV K ++ E + + G DI++ N+Y
Sbjct: 6 FTIHSHYKKARSGVITTAHGEIRTPAFMPVGTRGAVKAMLTEA--VVETGADILLGNTYH 63
Query: 67 IYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNREIVEF--------- 117
+ A G+HK + +D I DSG FQ+M + E V F
Sbjct: 64 LMLQPSAERIAYLGGLHKFMNWDKPILTDSGGFQVMSLSKLCKITEEGVSFRSHINGNKY 123
Query: 118 ----------QHRIGVDIGTFLDIPTPPDAPKEKAMEDLKIT---LERAREAEEIKE-IA 163
Q+ +G I LD TP + EKA + +T R+R+A +E A
Sbjct: 124 MLTPEYSTEIQYLLGSTITMALDECTPYPSTFEKAKTSMHLTTRWANRSRDAFVKREGYA 183
Query: 164 MNAAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRPD 223
IQGS Y +LR + + L +FE + IGG+ V+D V L +
Sbjct: 184 QFGIIQGSVYKELREQSVKDLVKCDFEGYAIGGLAVGEGQELMFRVLDYVPD---FLPQN 240
Query: 224 RPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEY------- 276
+P +L G G P AV+ G+D+FD ++ + T GT + +Y
Sbjct: 241 KPRYLMGVGKPSDIIGAVSRGIDMFDCVIPTRSGRNGQAFTKYGTVNIRNSKYADDKEPL 300
Query: 277 -FPCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIREGE 327
C CP C+ YT L + + L+ HNL + + RI+ I+ G+
Sbjct: 301 EHDCKCPACTNYTKAYLHHLVRIREILGPMLMTWHNLTYFQNLMSRIRTYIKLGK 355
>pir||D75256 queuine tRNA ribosyltransferase - Deinococcus radiodurans (strain
R1) >gi|6460406|gb|AAF12118.1|AE002087_1 (AE002087)
queuine tRNA ribosyltransferase [Deinococcus
radiodurans]
Length = 394
Score = 100 bits (246), Expect = 5e-20
Identities = 90/352 (25%), Positives = 148/352 (41%), Gaps = 39/352 (11%)
Query: 7 MLKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLI-VEPKELEKMGFDIIITNSY 65
+ +FE+K RDG R + + TP MPV + + P+EL ++G +I+ N+Y
Sbjct: 10 VFEFEIKQRDGRARTATFQTPRGAVTTPMFMPVGTQGTVKGISPQELLEIGSQMILANTY 69
Query: 66 -IIYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGN------------------ 106
++ + E KA G+ Y G DSG FQ+M G+
Sbjct: 70 HLMLRPGEQLVKA-HGGLPGFTAYPGPFLTDSGGFQVMSLGHMRKISEEGVVFKNHLDGS 128
Query: 107 -VDVSNREIVEFQHRIGVDIGTFLDI--PTPPDAPKEKAMEDLKIT-LERAREAEEIKEI 162
V+++ ++ Q +G D+ D P P + P +A D + LER + +
Sbjct: 129 RVELTPERSIQVQEALGADVIMAFDECPPYPAERPYIEASLDRTVRWLERCHAVKTKDDQ 188
Query: 163 AMNAAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRP 222
A+ A +QG + DLR + +GG L E+ V + L
Sbjct: 189 ALFAIVQGGVHEDLRLKSLEATLPFATPGFAVGG---LAVGESKEEMYPAVAFTAGRLPE 245
Query: 223 DRPVHLFGAGHPMVFALAVAMGVDLFD------SASYALYAKDDRYLTPEGTKRLDELEY 276
++P +L G GHP VA+G+D+FD + + DD L + +L+
Sbjct: 246 NKPRYLMGVGHPEDLVAGVALGIDMFDCVYPTRTGRFGYALTDDGRLNLNSSAPRTQLQP 305
Query: 277 F--PCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAI 323
C C C YT L + + E R+L++HNL + +ER++ AI
Sbjct: 306 IDAECDCYACRHYTRAYLAHLLRAEEMLAPRMLSLHNLRYLHRLVERMRVAI 357
>pir||H83169 queuine tRNA-ribosyltransferase PA3823 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9949997|gb|AAG07210.1|AE004799_16 (AE004799) queuine
tRNA-ribosyltransferase [Pseudomonas aeruginosa]
Length = 372
Score = 94.4 bits (231), Expect = 3e-18
Identities = 90/360 (25%), Positives = 149/360 (41%), Gaps = 37/360 (10%)
Query: 8 LKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLI-VEPKELEKMGFDIIITNSYI 66
+ FE+ A DG R G+L +ETPA MPV + + P+++E +G II+ N++
Sbjct: 1 MNFELLATDGKARRGRLTFPRGVVETPAFMPVGTYGTVKGMLPRDIEDIGAQIILGNTFH 60
Query: 67 IYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNREIVEF--------- 117
++ +H + + G I DSG FQ+ G + E V F
Sbjct: 61 LWLRPGTEVIQRHGDLHDFMQWKGPILTDSGGFQVFSLGAMRKIKEEGVTFASPVDGAKV 120
Query: 118 ----------QHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITL---ERAREAEEIKEIAM 164
Q +G DI D TP A + A ++++L +R++ A A+
Sbjct: 121 FMGPEESMAVQRALGSDIVMIFDECTPYPADHDVAKRSMELSLRWAKRSKIAHGDSPSAL 180
Query: 165 NAAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRPDR 224
+QG + DLR + L + F+ IGG L E++ ++ + D+
Sbjct: 181 FGIVQGGMHEDLRLRSLDGLQEIGFDGLAIGG---LSVGEPKEEMIRVLDFLPPQMPADK 237
Query: 225 PVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRL-------DELEYF 277
P +L G G P V GVD+FD A++ G ++ D+
Sbjct: 238 PRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFVDSGVIKIRNSVHKHDDSTLD 297
Query: 278 P-CSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIREGELWRLVD 333
P C C C ++ L + K + L IHNL + + +++AI++G L VD
Sbjct: 298 PTCDCYTCKHFSRAYLHHLDKCGEMLGSMLNTIHNLRHYQRVMAGLREAIQQGTLAAFVD 357
>sp|P44594|TGT_HAEIN QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANINE TRANSGLYCOSYLASE)
(GUANINE INSERTION ENZYME) >gi|1075397|pir||A64057
queuine tRNA-ribosyltransferase (EC 2.4.2.29) -
Haemophilus influenzae (strain Rd KW20)
>gi|1573209|gb|AAC21911.1| (U32710) tRNA-guanine
transglycosylase (tgt) [Haemophilus influenzae Rd]
Length = 382
Score = 93.2 bits (228), Expect = 7e-18
Identities = 89/361 (24%), Positives = 151/361 (41%), Gaps = 49/361 (13%)
Query: 8 LKFEVKARDGAGRIGKLEVNGKK----IETPAIMPVVNPKQLI-VEPKELEKMGFDIIIT 62
+K+E+ G R G+L + +ETPA MPV + + P+E+ G +I++
Sbjct: 1 MKYELDKTSGNARRGRLVFERPQGTFSVETPAFMPVGTYGTVKGMTPEEVRATGAEILLG 60
Query: 63 NSYIIYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNREIVEFQHRI- 121
N++ ++ +H + + I DSG FQ+ G + E V+FQ+ I
Sbjct: 61 NTFHLWLRPGQEVMRKHGDLHDFMQWHRPILTDSGGFQVFSLGKLRKITEEGVKFQNPIN 120
Query: 122 ------------------GVDIGTFLDIPTPPDAPKEKAMEDLKITLERA-REAEEIKEI 162
G DI D TP A + A + ++++L A R + E+
Sbjct: 121 GERIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAKKSMEMSLRWAKRSRDRFDEL 180
Query: 163 ----AMNAAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVV---PLLEAYRFREVVDIVIS 215
A+ IQG + +LR+ + L ++ F+ + +GG+ P + +R E + I
Sbjct: 181 GNKNALFGIIQGGVFEELRKVSLEGLVNIGFDGYAVGGLAVGEPKEDMHRILEYICPQIP 240
Query: 216 SKMALRPDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELE 275
+ D+P +L G G P V G+D+FD A++ +G ++ +
Sbjct: 241 A------DKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGIVKIRNAK 294
Query: 276 YF--------PCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIR 324
Y C C C YT L + K ARL IHNL + + I+QAI
Sbjct: 295 YRDDTSPLDPECDCYTCKNYTKAYLYHLDKCGEILGARLNTIHNLRYYQRLMAEIRQAIE 354
Query: 325 E 325
+
Sbjct: 355 D 355
>sp|O67331|TGT_AQUAE QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANINE TRANSGLYCOSYLASE)
(GUANINE INSERTION ENZYME) >gi|7448763|pir||H70412
queuine tRNA-ribosyltransferase (EC 2.4.2.29) - Aquifex
aeolicus >gi|2983726|gb|AAC07288.1| (AE000733) queuine
tRNA-ribosyltransferase [Aquifex aeolicus]
Length = 378
Score = 90.1 bits (220), Expect = 6e-17
Identities = 96/364 (26%), Positives = 159/364 (43%), Gaps = 49/364 (13%)
Query: 7 MLKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQL-IVEPKELEKMGFDIIITNSY 65
M KF + +DG R G++ IETP MPV + + K L+++G II+ N+Y
Sbjct: 1 MFKFHLITKDGKARRGRIYTPHGVIETPVFMPVGTQGTVKAMLHKLLDEIGTQIILGNTY 60
Query: 66 IIY--KDRELREKALEVGIHKLLGYDGIIEVDSGSFQLM-----RYGN----VDVSN--- 111
+Y E+ +KA G+H+ + ++ I DSG +Q+ ++GN V VS+
Sbjct: 61 HLYLRPGTEIIKKA--GGLHRFISWNKPILTDSGGYQVFSLAKGKFGNRKAKVKVSDEGV 118
Query: 112 ---------------REIVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKIT---LERA 153
++VE Q G DI LD K A + LK T LER+
Sbjct: 119 EFQDHLQGDKHFFTPEKVVEIQEIFGSDIMMPLDECVEYPVDKNYAEKALKRTINWLERS 178
Query: 154 REAEEIKEIAMNAAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIV 213
+A++ ++ + +QG+ + DLR+ A ++ F+ G + L +E++ +
Sbjct: 179 IKAKKREDQVLFGIVQGAFWKDLRKKAVE--ETLKFDEFLFGYSIGGLSVGEPKEIMYGM 236
Query: 214 ISSKMALRPD-RPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLD 272
L P+ +P +L G G P AV GVD+FD A+ T +G +
Sbjct: 237 TEVVCELLPEKKPRYLMGVGKPEDILEAVERGVDMFDCVVPTRNARTGTLYTSQGVVDIR 296
Query: 273 ELEYF--------PCSCPVCSRYTPQELREMPKEERAR---LLAIHNLWVIKEEIERIKQ 321
++ C C C ++ LR + E L IHNL + +E +++
Sbjct: 297 HSKWKEDFSPLDPECDCYTCRNFSKAYLRHLFVAEEISAYVLNTIHNLRFYLKMMEEVRK 356
Query: 322 AIRE 325
AI E
Sbjct: 357 AIEE 360
>sp|Q9ZMF4|TGT_HELPJ QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANINE TRANSGLYCOSYLASE)
(GUANINE INSERTION ENZYME) >gi|7448766|pir||C71952
queuine tRNA-ribosyltransferase - Helicobacter pylori
(strain J99) >gi|4154790|gb|AAD05847.1| (AE001464)
QUEUINE TRNA-RIBOSYLTRANSFERASE [Helicobacter pylori
J99]
Length = 371
Score = 89.3 bits (218), Expect = 1e-16
Identities = 92/361 (25%), Positives = 157/361 (43%), Gaps = 52/361 (14%)
Query: 8 LKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNP---KQLIVEPKELEKMGFDIIITNS 64
+ F+++A D R G L + ++ETP MPV K L + E +G +I+ N+
Sbjct: 1 MDFQLQAVDDNARAGVLNLAHSQVETPVFMPVGTQGCIKSLDAMDAQ-EILGAKLILANT 59
Query: 65 YIIYKDRELR--EKALEV--GIHKLLGYDGIIEVDSGSFQ--------------LMRYGN 106
Y +Y LR EK +E G+H+ + G DSG FQ ++ +
Sbjct: 60 YHMY----LRPGEKVVEQLGGLHRFAQFQGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSH 115
Query: 107 VDVSNR-----EIVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITLERAREAEEIKE 161
+D S ++++ Q+ + DI LD AP ++ E +K + + A + E +
Sbjct: 116 IDGSKHLFTPIKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHK 175
Query: 162 IA------MNAAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVIS 215
+ A IQG T+ +R + L+ F+ + IGG L E+++ +
Sbjct: 176 ENNRPNNNLFAIIQGGTHLKMRSLSVG-LTHEGFDGYAIGG---LAVGESVDEMLETIAH 231
Query: 216 SKMALRPDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELE 275
+ L D+P +L G G P A+++GVD+FD A++ T G +
Sbjct: 232 TAPLLPKDKPRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAP 291
Query: 276 Y--------FPCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIR 324
Y C+C C RY+ L + + + ARL ++HNL E ++ + AI
Sbjct: 292 YKLDDTPIEENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAIL 351
Query: 325 E 325
E
Sbjct: 352 E 352
>sp|O08314|TGT_HELPY QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANINE TRANSGLYCOSYLASE)
(GUANINE INSERTION ENZYME) >gi|7448767|pir||A64555
tRNA-guanine transglycosylase - Helicobacter pylori
(strain 26695) >gi|2313378|gb|AAD07350.1| (AE000547)
tRNA-guanine transglycosylase (tgt) [Helicobacter pylori
26695]
Length = 371
Score = 87.8 bits (214), Expect = 3e-16
Identities = 93/361 (25%), Positives = 157/361 (42%), Gaps = 52/361 (14%)
Query: 8 LKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNP---KQLIVEPKELEKMGFDIIITNS 64
+ F+++A D R G L + ++ TP MPV K L + E +G +I+ N+
Sbjct: 1 MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQ-EILGAKLILANT 59
Query: 65 YIIYKDRELR--EKALEV--GIHKLLGYDGIIEVDSGSFQ--------------LMRYGN 106
Y +Y LR EK +E G+H+ + G DSG FQ ++ +
Sbjct: 60 YHMY----LRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSH 115
Query: 107 VDVSNR-----EIVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITLERAR---EAEE 158
+D S ++++ Q+ + DI LD AP ++ E +K + + A E +
Sbjct: 116 IDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHK 175
Query: 159 IKEIAMN---AAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVIS 215
K N A IQG T+ +R + L+ F+ + IGG+ A E+++ +
Sbjct: 176 EKNRPSNNLFAIIQGGTHLKMRSLSVG-LTHEGFDGYAIGGLAVGESA---DEMLETIAH 231
Query: 216 SKMALRPDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELE 275
+ L D+P +L G G P A+++GVD+FD A++ T G +
Sbjct: 232 TAPLLPKDKPRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAP 291
Query: 276 Y--------FPCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIR 324
Y C+C C RY+ L + + + ARL ++HNL E ++ + AI
Sbjct: 292 YKLDNTPIEENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAIL 351
Query: 325 E 325
E
Sbjct: 352 E 352
>sp|Q23623|TGT_CAEEL PUTATIVE QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANINE
TRANSGLYCOSYLASE) (GUANINE INSERTION ENZYME)
>gi|7511343|pir||T28024 hypothetical protein ZK829.6 -
Caenorhabditis elegans >gi|3881825|emb|CAA98076.1|
(Z73899) Similarity to Haemophilus Queuine
tRNA-ribosyltransferase (SW:TGT_HAEIN), contains
similarity to Pfam domain: PF01702 (Queuine
tRNA-ribosyltransferase), Score=501.7, E-value=1.8e-147,
N=1~cDNA EST yk673g6.3 comes from this gene~cDNA EST
yk673g>
Length = 400
Score = 87.8 bits (214), Expect = 3e-16
Identities = 91/358 (25%), Positives = 154/358 (42%), Gaps = 43/358 (12%)
Query: 8 LKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLI-VEPKELEKMGFDIIITNSYI 66
++++V AR G R G L + +ETP MPV + + P++L M I++ N+Y
Sbjct: 1 MRYDVLARAGFARRGNLHLPHSIVETPVFMPVGTQGTMKGIVPEQLVSMDCRILLCNTYH 60
Query: 67 I-YKDRELREKALEVGIHKLLGYDGIIEVDSGSFQ---LMRYGNVDVSN----------- 111
+ ++ R KA G+HK++ ++ I DSG FQ L + VD +
Sbjct: 61 LGHRPGHERVKAAG-GLHKMMNWNRSILTDSGGFQMVSLSKLMTVDENGVNFESPHTGEM 119
Query: 112 -----REIVEFQHRIGVDIGTFLD----IPTPPDAPKEKAMEDLKITLERAREAEEIKEI 162
+ +E Q +G DI LD + T D KE ++ L+R + A +
Sbjct: 120 MALPPEKSIEIQQALGADIMMQLDHVIHVLTTGDIVKEAMHRSIR-WLDRCKVAHTRDDQ 178
Query: 163 AMNAAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRP 222
AM +QG +LR+ A+ ++ IGG+ E F VV + AL P
Sbjct: 179 AMFPILQGGLNLELRKECAKEMAKRAKVGIAIGGLSGGEEKDHFWRVVAACCA---ALPP 235
Query: 223 DRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEG------TKRLDELEY 276
P ++ G G P+ + +G D+FD A+ + G KR E ++
Sbjct: 236 HLPRYVMGVGFPVDLVICSFLGADMFDCVYPTRTARFGTAMVRRGGLMQLNQKRYKE-DF 294
Query: 277 FP----CSCPVCSRYTPQELREMPKEER--ARLLAIHNLWVIKEEIERIKQAIREGEL 328
P C C C YT + + +E L+++HN+ + + ++QAI+ +
Sbjct: 295 LPIDKKCECNTCKNYTRAYIHSIVGKETVGCHLVSVHNIKHQLDLMRDVRQAIQSNSV 352
>sp|Q9VPY8|TGT_DROME PROBABLE QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANINE
TRANSGLYCOSYLASE) (GUANINE INSERTION ENZYME)
>gi|7296102|gb|AAF51396.1| (AE003587) CG4947 gene
product [Drosophila melanogaster]
Length = 427
Score = 87.4 bits (213), Expect = 4e-16
Identities = 88/356 (24%), Positives = 146/356 (40%), Gaps = 37/356 (10%)
Query: 20 RIGKLEVNGKKIETPAIMPVVNPKQLI-VEPKELEKMGFDIIITNSYIIYKDRELREKAL 78
R G + + ++ TP MPV L + P +L ++ I++ N+Y + +
Sbjct: 23 RAGLMTLRHSEVNTPVFMPVGTQGTLKGIVPDQLIELNCQILLGNTYHLGLRPGIETLKK 82
Query: 79 EVGIHKLLGYDGIIEVDSGSFQ---LMRYGNVD----------------VSNREIVEFQH 119
G+HK +G+ I DSG FQ L++ +D ++ +E Q+
Sbjct: 83 AGGLHKFMGWPRAILTDSGGFQMVSLLQLAEIDEHGVNFRSPFDNSQCMLTPEHSIEIQN 142
Query: 120 RIGVDIGTFLD--IPTPPDAPK-EKAMEDLKITLERAREAE-EIKEIAMNAAIQGSTYTD 175
IG DI LD + T P+ E+AME ++R EA + ++ +QG
Sbjct: 143 AIGGDIMMQLDDVVKTTTTGPRVEEAMERTIRWVDRCIEAHARDDDQSLFPIVQGGLDVP 202
Query: 176 LRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRPDRPVHLFGAGHPM 235
LR+ L +GG+ + F +VD+ L D+P +L G G
Sbjct: 203 LRQRCVSALMERQVRGFAVGGLSGGESKHDFWRMVDVCTGY---LPKDKPRYLMGVGFAA 259
Query: 236 VFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEY--------FPCSCPVCSRY 287
+ VA+G+D+FD A+ L G L + +Y C C C RY
Sbjct: 260 DLVVCVALGIDMFDCVFPTRTARFGCALVDSGQLNLKQPKYKLDMEPIDKDCDCSTCRRY 319
Query: 288 TPQELREMPKEE--RARLLAIHNLWVIKEEIERIKQAIREGELWRLVDERARSHPK 341
T L + E + LL+IHN+ + +++AI+ E + V + H K
Sbjct: 320 TRSYLHHIATNESVSSSLLSIHNVAYQLRLMRSMREAIQRDEFPQFVADFMARHFK 375
>sp|Q55983|TGT_SYNY3 QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANINE TRANSGLYCOSYLASE)
(GUANINE INSERTION ENZYME) >gi|7448765|pir||S77072
queuine tRNA-ribosyltransferase (EC 2.4.2.29) -
Synechocystis sp. (strain PCC 6803)
>gi|1006609|dbj|BAA10764.1| (D64005) transfer
RNA-guanine transglycosylase [Synechocystis sp.]
Length = 374
Score = 84.3 bits (205), Expect = 3e-15
Identities = 83/353 (23%), Positives = 140/353 (39%), Gaps = 48/353 (13%)
Query: 11 EVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLI-VEPKELEKMGFDIIITNSYIIYK 69
+ + R G + +ETP MPV + + P +L +++ N+Y ++
Sbjct: 13 QARCSHSQARAGIFQTPHGPVETPRFMPVGTLATVKGITPAQLADTQAQMVLANTYHLHL 72
Query: 70 DRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGN-------------------VDVS 110
+ A G+HK +G+ G + DSG FQ+ +D++
Sbjct: 73 QPGEKIIAAAGGVHKFMGWSGPMLTDSGGFQVFSLSQLRTIGEQGVLFRSPRDGRLIDMT 132
Query: 111 NREIVEFQHRIGVDIGTFLDIPTPPDAPK---EKAMEDLKITLERAREAEEIK-EIAMNA 166
+ Q+ +G D+ D P + E+A LER EA + + A+
Sbjct: 133 PENSIRIQNALGADVIMAFDECPPGQGDRLMVEQATARTYRWLERCIEAHQRPGDQALFG 192
Query: 167 AIQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRP-DRP 225
+QG TY DLR AA +L +++ + IGGV ++D V+ L P +P
Sbjct: 193 IVQGGTYLDLRSQAAEQLIALDLPGYAIGGV----SVGEAPALIDKVVQHTAPLLPAHKP 248
Query: 226 VHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTP------------EGTKRLDE 273
+L G G A A+ G+DLFD + + L E + LDE
Sbjct: 249 RYLMGVGTYREMARAIFAGIDLFDCVIPTRFGRHGTALVQGERWNLKNATFREDHRPLDE 308
Query: 274 LEYFPCSCPVCSRYTPQELREMPKEERAR---LLAIHNLWVIKEEIERIKQAI 323
C C C ++ L + + LL++HN+ + + I+QAI
Sbjct: 309 ----TCPCYTCQTFSRAYLNHLIRSREMLGYILLSLHNITELIRFTQSIRQAI 357
>emb|CAA72784.1| (Y12061) tRNA-guanine transglycosylase [Helicobacter pylori]
Length = 371
Score = 83.5 bits (203), Expect = 6e-15
Identities = 89/365 (24%), Positives = 154/365 (41%), Gaps = 60/365 (16%)
Query: 8 LKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLIVEPKELEK------MGFDIII 61
+ F+++A D R G L++ ++ TP MPV + K L+ +G +I+
Sbjct: 1 MDFQLQATDKHARAGLLDLAHSQVATPVFMPVGTQGCI----KSLDATDVQGILGAKLIL 56
Query: 62 TNSYIIYKDRELREKALEVG----IHKLLGYDGIIEVDSGSFQ--------------LMR 103
N+Y +Y LR VG +H + G DSG FQ ++
Sbjct: 57 ANTYHMY----LRPGEKVVGQLGVLHHFAQFQGSFLTDSGGFQAFSLSDNVKLQEGGIVF 112
Query: 104 YGNVDVSNR-----EIVEFQHRIGVDIGTFLDIPTPPDAPKEKAMEDLK-------ITLE 151
++D +N ++++ Q+ + DI LD AP ++ E +K ++LE
Sbjct: 113 KSHIDGNNHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANLSLE 172
Query: 152 RAREAEEIKEIAMNAAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVD 211
+E + A IQG T+ +R + L+ F+ + IGG+ A E+++
Sbjct: 173 YHKENNRPNN-NLFAIIQGGTHLKMRSLSVG-LTHKGFDGYAIGGLAVGESA---DEMLE 227
Query: 212 IVISSKMALRPDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRL 271
+ + L D+P +L G G P A+ +GVD+FD A++ T G +
Sbjct: 228 TIAHTTPLLPKDKPRYLMGVGTPENILDAIGLGVDMFDCVMPTRNARNATLFTHSGKISI 287
Query: 272 DELEY--------FPCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIK 320
Y C+C C RY+ L + + + ARL ++HNL E ++ +
Sbjct: 288 KNAPYKLDNTPIEENCTCYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNAR 347
Query: 321 QAIRE 325
AI E
Sbjct: 348 NAILE 352
>gb|AAG20138.1| (AE005092) archaeosine tRNA-ribosyltransferase; TgtA2
[Halobacterium sp. NRC-1]
Length = 585
Score = 83.1 bits (202), Expect = 8e-15
Identities = 57/218 (26%), Positives = 107/218 (48%), Gaps = 17/218 (7%)
Query: 211 DIVISSKMALRPDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKR 270
D ++ ++ A+ D + L G P A+ V GVDL ++ + RYLT EG
Sbjct: 121 DAIVEARAAIPDDTALSLSGVATPRNVAMLVYAGVDLVNADRAVVRGTQGRYLTTEGAYD 180
Query: 271 LDELEYFPCSCPVCSRYTPQELREMPKEERARLLAI-HNLWVIKEEIERIKQAIREGELW 329
L +L+ PCSCP C + P + R + HN+ ++ E+ R+++ IR+G L
Sbjct: 181 LADLDELPCSCPAC---------QQPAADFDRTDCVDHNVNALEAELARVRRRIRDGRLR 231
Query: 330 RLVDERARSHPKLYSAYKRLLDHYTFLEEFEPVTKKSAVFKISHESLRWPLVRRARERAE 389
V+ +AR L +A + L Y ++E+ P + S + + ++L ++R A+
Sbjct: 232 DYVEGQARHTAWLTAAMRELDQQYGYVEQRTPTFRDSLLLSSTQDALHRVEIQRF---AD 288
Query: 390 RVNSKFGDLVE-HPIFGKVTKYLTLTYPFAQSEAEDEF 426
RV S++ ++ HP+ + + P++ S++ +F
Sbjct: 289 RVTSRYRKRLDGHPL---LLVPCSARKPYSDSQSHRQF 323
>sp|P57233|TGT_BUCAI QUEUINE TRNA-RIBOSYLTRANSFERASE (TRNA-GUANINE TRANSGLYCOSYLASE)
(GUANINE INSERTION ENZYME) >gi|10038816|dbj|BAB12851.1|
(AP001118) queuine tRNA-ribosyltransferase [Buchnera sp.
APS]
Length = 370
Score = 81.9 bits (199), Expect = 2e-14
Identities = 84/356 (23%), Positives = 140/356 (38%), Gaps = 45/356 (12%)
Query: 8 LKFEVKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQL-IVEPKELEKMGFDIIITNSYI 66
+ F+V +D R G N + IETP MPV + + +E++ G II++N++
Sbjct: 1 MNFKVLYQDNNARCGVFNFNQEIIETPVFMPVGTYGAVKSISTEEIKNTGSRIILSNAFH 60
Query: 67 IYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNREIVEFQHRI----- 121
+Y L L +H + + G I DSG FQ+ N E V FQ+ I
Sbjct: 61 LYFRPGLEIIKLHGNLHNFMNWSGPILTDSGGFQVFSLSRFCKVNEEGVIFQNHIDGKKT 120
Query: 122 --------------GVDIGTFLDIPTPPDAPKEKAMEDLKITLERAREAEEIKEIAMN-- 165
G +I D + EK ++ +L A+++ + N
Sbjct: 121 FLTPKISMKIQSDLGSNIVMIFDQCIEYNQNWEKTKNAMERSLYWAKKSRIYFDSYKNKN 180
Query: 166 ---AAIQGSTYTDLRRYAARRLSSMNFEIHPIGGVV---PLLEAYRFREVVDIVISSKMA 219
I G Y LR + + L ++F+ + +GG+ P +E Y+ + + I
Sbjct: 181 SLFGIIHGGIYPSLRDISLQELIKIDFDGYALGGLAVGEPKIEMYKLLDHICPQIPK--- 237
Query: 220 LRPDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEY--- 276
++P +L G G P V GVD+FD A++ G ++ +Y
Sbjct: 238 ---NKPRYLMGVGKPEDLIEGVRRGVDMFDCVIPTRNARNGHLFVTNGVIKIRNKKYKKD 294
Query: 277 -----FPCSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIR 324
C C C Y+ L + ARL IHNL + + I+ +I+
Sbjct: 295 LSCLDNTCVCYTCRYYSRSYLHHLDACNEILGARLNTIHNLHYYQTLMSNIRNSIK 350
>pir||G81302 queuine tRNA-ribosyltransferase (EC 2.4.2.29) Cj1010 [imported] -
Campylobacter jejuni (strain NCTC 11168)
>gi|6968446|emb|CAB73266.1| (AL139077) queuine
tRNA-ribosyltransferase [Campylobacter jejuni]
Length = 373
Score = 75.3 bits (182), Expect = 2e-12
Identities = 86/358 (24%), Positives = 136/358 (37%), Gaps = 45/358 (12%)
Query: 8 LKFEVKARDGAGRIGKLEVNGKKIETPAIMPV--VNPKQLIVEPKELEKMGFDIIITNSY 65
++F++K +DG R+ ++ TP MPV V + + ++ II+ N+Y
Sbjct: 1 MEFKLKHKDGMARVCEITTAHSTFLTPVFMPVGTVGAVKSLDANDMKNELDAKIILANTY 60
Query: 66 IIYKDRELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVDVSNREIVEF-------- 117
+Y + G+H +D DSG FQ E +EF
Sbjct: 61 HMYLRPTSKVVKDFGGLHGFTKFDRSFLTDSGGFQAFSLSKNSKHFNEGIEFKSHIDGSR 120
Query: 118 -----------QHRIGVDIGTFLDIPTPPDAPKEKAMEDLKITLERAREAEEIKEIAMNA 166
Q+ DI LD A KE+ + T+ A+EA + N
Sbjct: 121 HLFTPKSVLDTQYDFNSDIMMILDDLVALPATKERVKISVDRTILWAKEAITYHKNMQNK 180
Query: 167 AI----------QGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISS 216
I QG T + R+ A L+ M F+ IGG+ E E V +
Sbjct: 181 GIGIGQNIFGIIQGGTDYEERKRCALSLNEMPFDGLAIGGLSVGEENALMYETVQ---NL 237
Query: 217 KMALRPDRPVHLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEY 276
L +RP +L G G P V GVD+FD A++ + T G + + E+
Sbjct: 238 NPYLDENRPRYLMGVGTPEDLVENVERGVDMFDCVMPTRNARNGTFFTSFGKFNIKKAEF 297
Query: 277 F--------PCSCPVCSRYTPQELREMPKEERA---RLLAIHNLWVIKEEIERIKQAI 323
CSC C ++ L + K + RL ++HNL E ++++AI
Sbjct: 298 INDHEVIDSTCSCYTCRNFSRGYLNHLFKAKELTFFRLASLHNLHYYLELARKMREAI 355
>pir||F72505 hypothetical protein APE2020 - Aeropyrum pernix (strain K1)
>gi|5105718|dbj|BAA81030.1| (AP000063) 146aa long
hypothetical protein [Aeropyrum pernix]
Length = 146
Score = 74.5 bits (180), Expect = 3e-12
Identities = 45/115 (39%), Positives = 60/115 (52%), Gaps = 7/115 (6%)
Query: 447 QFGENASKAF---EGAEVELARTGMPRQVKLNGKRLATVRAEDGFLTLGIEGAKRLHKVL 503
QF A+ A EG EV G RQV G+ T+RA DG+ +L + L + +
Sbjct: 16 QFSPEAADALLSVEGLEVSFGAGGRVRQVWAGGEPYLTLRAGDGYFSLSTRAGEFLRRSI 75
Query: 504 EYPRMRVVVSEEAEPFARKGKDVFAKFVLFADPGIRPYDEVLVVNEKDELLATGQ 558
PR RV+V EP VFAK V+ D G+RP DE +VV+E+D LL G+
Sbjct: 76 AKPRFRVIVGSGVEPVG----SVFAKQVVGGDEGLRPGDEAIVVDEEDRLLGVGR 126
>prf||1819268A tRNA guanine transglycosylase [Zymomonas mobilis]
Length = 247
Score = 72.6 bits (175), Expect = 1e-11
Identities = 64/243 (26%), Positives = 111/243 (45%), Gaps = 23/243 (9%)
Query: 115 VEFQHRIGVDIGTFLDIPTPPDAP---KEKAMEDLKITLERAREA-EEIKEIAMNAAI-- 168
+E QH +G DI D TP A + +ME +R+R+A + KE A NAA+
Sbjct: 2 IEIQHLLGSDIVMAFDECTPYPASLRARASSMERSMRWAKRSRDAFDSRKEQAENAALFG 61
Query: 169 --QGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRPDRPV 226
QGS + +LR+ +A L+ + F+ + +GG+ V+D + L D+P
Sbjct: 62 IQQGSVFENLRQQSADALAEIGFDGYAVGGLAVGEGQDEMFRVLDFSVPM---LPDDKPH 118
Query: 227 HLFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEY--------FP 278
+L G G P AV G+D+FD ++ + T +G + +
Sbjct: 119 YLMGVGKPDDIVGAVERGIDMFDCVLPTRSGRNGQAFTWDGPINIRNARFSEDLKPLDSE 178
Query: 279 CSCPVCSRYTPQELREMPKEER---ARLLAIHNLWVIKEEIERIKQAIREGELWRLV-DE 334
C C VC +++ + + + A L+ HN+ ++ +++I+ +I EG + D
Sbjct: 179 CHCAVCQKWSRAYIHHLIRAGEILGAMLMTEHNIAFYQQLMQKIRDSISEGRFSQFAQDF 238
Query: 335 RAR 337
RAR
Sbjct: 239 RAR 241
>gi|11230788 queuine tRNA-ribosyltransferase [Mus musculus]
>gi|7415808|dbj|BAA93550.1| (AB034632) tRNA-guanine
transglycosylase [Mus musculus]
Length = 387
Score = 72.6 bits (175), Expect = 1e-11
Identities = 84/349 (24%), Positives = 145/349 (41%), Gaps = 43/349 (12%)
Query: 14 ARDGAGRIGKLEVNGKKIETPAIMPVVNPKQLI-VEPKELEKMGFDIIITNSYII--YKD 70
+R GA R G+L + + TP MPV + + ++L+ +G I + N+Y +
Sbjct: 8 SRSGA-RAGELRLPHGTVATPVFMPVGTQATMKGITTEQLDSLGCRICLGNTYHLGLRPG 66
Query: 71 RELREKALEVGIHKLLGYDGIIEVDSGSFQLMRYGNVD-------------------VSN 111
EL KA G+H + + + DSG FQ++ ++ +S
Sbjct: 67 PELIRKAQ--GLHGFMNWPHNLLTDSGGFQMVSLFSLSEVTEEGVHFRSPYDGEETLLSP 124
Query: 112 REIVEFQHRIGVDIGTFLD--IPTPPDAPK-EKAMEDLKITLERAREAEEIKEIA-MNAA 167
VE Q+ +G DI LD + + P E+AM L+R A + + + A
Sbjct: 125 ERSVEIQNALGSDIIMQLDHVVSSTVTGPLVEEAMHRSVRWLDRCIAAHKHPDKQNLFAI 184
Query: 168 IQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLEAYRFREVVDIVISSKMALRPDRPVH 227
IQG DLR + ++ + IGG+ +F ++V + S L D+P +
Sbjct: 185 IQGGLNADLRTTCLKEMTKRDVPGFAIGGLSGGESKAQFWKMVAL---STSMLPKDKPRY 241
Query: 228 LFGAGHPMVFALAVAMGVDLFDSASYALYAKDDRYLTPEGTKRLDELEYF--------PC 279
L G G+ + VA+G D+FD A+ L P G +L + +Y C
Sbjct: 242 LMGVGYATDLVVCVALGCDMFDCVYPTRTARFGSALVPTGNLQLKKKQYAKDFSPINPEC 301
Query: 280 SCPVCSRYTPQELREMPKEERARL---LAIHNLWVIKEEIERIKQAIRE 325
CP C ++ L + + L +HN+ + + ++ +I E
Sbjct: 302 PCPTCQTHSRAFLHALLHSDNTTALHHLTVHNIAYQLQLLSAVRSSILE 350
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.320 0.139 0.395
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 210781384
Number of Sequences: 2977
Number of extensions: 9138568
Number of successful extensions: 22338
Number of sequences better than 1.0e-10: 45
Number of HSP's better than 0.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 34
Number of HSP's that attempted gapping in prelim test: 22190
Number of HSP's gapped (non-prelim): 52
length of query: 584
length of database: 189,106,746
effective HSP length: 58
effective length of query: 526
effective length of database: 154,394,500
effective search space: 81211507000
effective search space used: 81211507000
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 167 (69.5 bits)