BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB0742 (pflX) DE:pyruvate formate-lyase activating enzyme (pflX) (335 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||B75091 pyruvate formate-lyase activating enzyme (pflx) PAB0... 699 0.0 pir||B71052 hypothetical protein PH1113 - Pyrococcus horikoshii ... 577 e-164 emb|CAA58794.1| (X83963) pyruvate formate lyase activating prote... 542 e-153 gi|11499543 pyruvate formate-lyase activating enzyme (pflX) [Arc... 326 3e-88 pir||H69008 conserved hypothetical protein MTH1069 - Methanobact... 320 2e-86 sp|Q58087|Y674_METJA HYPOTHETICAL PROTEIN MJ0674 >gi|2128025|pir... 312 5e-84 pir||H72634 probable pyruvate formate lyase activating protein A... 286 3e-76 pir||A70364 conserved hypothetical protein aq_729 - Aquifex aeol... 160 2e-38 pir||D72416 hypothetical protein TM0109 - Thermotoga maritima (s... 158 1e-37 pir||E72317 conserved hypothetical protein - Thermotoga maritima... 129 5e-29 gb|AAG23565.1|AF244616_1 (AF244616) hypothetical protein [Carbox... 99 5e-20 pir||G75119 pyruvate formate-lyase activating enzyme related pro... 80 5e-14 pir||A71012 hypothetical protein PH1391 - Pyrococcus horikoshii ... 79 6e-14 pir||F69230 pyruvate formate-lyase activating enzyme related pro... 72 8e-12 >pir||B75091 pyruvate formate-lyase activating enzyme (pflx) PAB0742 - Pyrococcus abyssi (strain Orsay) >gi|5458543|emb|CAB50031.1| (AJ248286) pyruvate formate-lyase activating enzyme (pflX) [Pyrococcus abyssi] Length = 335 Score = 699 bits (1785), Expect = 0.0 Identities = 335/335 (100%), Positives = 335/335 (100%) Query: 1 MGAREALPKYFAILEGDDVPNFMLAKKVEVKPEDSIEEMWNSHEEGMDKLRENDIKDNPS 60 MGAREALPKYFAILEGDDVPNFMLAKKVEVKPEDSIEEMWNSHEEGMDKLRENDIKDNPS Sbjct: 1 MGAREALPKYFAILEGDDVPNFMLAKKVEVKPEDSIEEMWNSHEEGMDKLRENDIKDNPS 60 Query: 61 FSLLDLKAVIANKILESCTLCEIKCRVNRKESVGYCRVKETLIASDFLHYGEEPELVPSY 120 FSLLDLKAVIANKILESCTLCEIKCRVNRKESVGYCRVKETLIASDFLHYGEEPELVPSY Sbjct: 61 FSLLDLKAVIANKILESCTLCEIKCRVNRKESVGYCRVKETLIASDFLHYGEEPELVPSY 120 Query: 121 TVFFSGCNFRCVFCQNWDISQFRVGLNYRPEYMALKIERAYRFGAKNVNFVGGEPTPNLP 180 TVFFSGCNFRCVFCQNWDISQFRVGLNYRPEYMALKIERAYRFGAKNVNFVGGEPTPNLP Sbjct: 121 TVFFSGCNFRCVFCQNWDISQFRVGLNYRPEYMALKIERAYRFGAKNVNFVGGEPTPNLP 180 Query: 181 FILETLRYVNVPIPVVWNSNMYMSEDSMKLLDGIVDIYLGDFKYGNDRCALKYSKVPRYW 240 FILETLRYVNVPIPVVWNSNMYMSEDSMKLLDGIVDIYLGDFKYGNDRCALKYSKVPRYW Sbjct: 181 FILETLRYVNVPIPVVWNSNMYMSEDSMKLLDGIVDIYLGDFKYGNDRCALKYSKVPRYW 240 Query: 241 EVVTRNFLLAKNHFRAEFLIRHLVMPNHLECCTRPILKWISENLGKDVRVNVMFQYRPEY 300 EVVTRNFLLAKNHFRAEFLIRHLVMPNHLECCTRPILKWISENLGKDVRVNVMFQYRPEY Sbjct: 241 EVVTRNFLLAKNHFRAEFLIRHLVMPNHLECCTRPILKWISENLGKDVRVNVMFQYRPEY 300 Query: 301 KAMEYEEISRMLVLEEMEEAERLVREFGLKNALVG 335 KAMEYEEISRMLVLEEMEEAERLVREFGLKNALVG Sbjct: 301 KAMEYEEISRMLVLEEMEEAERLVREFGLKNALVG 335 >pir||B71052 hypothetical protein PH1113 - Pyrococcus horikoshii >gi|3257529|dbj|BAA30212.1| (AP000005) 337aa long hypothetical protein [Pyrococcus horikoshii] Length = 337 Score = 577 bits (1472), Expect = e-164 Identities = 261/337 (77%), Positives = 309/337 (91%), Gaps = 2/337 (0%) Query: 1 MGAREALPKYFAILEGDDVPNFMLAKKVEVK--PEDSIEEMWNSHEEGMDKLRENDIKDN 58 MGA EALPKYFAIL+ ++ PNFMLA+ V+V+ ++ ++ +W H+EG+DKL+END+ +N Sbjct: 1 MGAEEALPKYFAILKREEEPNFMLARNVKVRFRGDEDLDGLWVIHDEGLDKLKENDLTEN 60 Query: 59 PSFSLLDLKAVIANKILESCTLCEIKCRVNRKESVGYCRVKETLIASDFLHYGEEPELVP 118 P +LLDLKA IA+KILE CTLCEIKCRVNRK+S GYCRVKE+L+ASDFLHYGEEPELVP Sbjct: 61 PERNLLDLKAKIADKILERCTLCEIKCRVNRKKSPGYCRVKESLVASDFLHYGEEPELVP 120 Query: 119 SYTVFFSGCNFRCVFCQNWDISQFRVGLNYRPEYMALKIERAYRFGAKNVNFVGGEPTPN 178 SYTVFFSGCNFRCVFCQNWDISQFR+G+ Y PEYMA+KIE+A++ GA+NVNFVGGEPTPN Sbjct: 121 SYTVFFSGCNFRCVFCQNWDISQFRIGVEYLPEYMAIKIEKAFQLGARNVNFVGGEPTPN 180 Query: 179 LPFILETLRYVNVPIPVVWNSNMYMSEDSMKLLDGIVDIYLGDFKYGNDRCALKYSKVPR 238 LPFILETLRYV VPIP+VWNSNMYMSE++MKLLDG+VDIYLGDFKYGNDRCA+KYSKVPR Sbjct: 181 LPFILETLRYVRVPIPIVWNSNMYMSEEAMKLLDGVVDIYLGDFKYGNDRCAMKYSKVPR 240 Query: 239 YWEVVTRNFLLAKNHFRAEFLIRHLVMPNHLECCTRPILKWISENLGKDVRVNVMFQYRP 298 YWEVVTRNFLLAK HFRAEFLIRHLVMPNHL+CCT+PIL+WI+ENLG+++RVNVMFQYRP Sbjct: 241 YWEVVTRNFLLAKEHFRAEFLIRHLVMPNHLDCCTKPILRWIAENLGREIRVNVMFQYRP 300 Query: 299 EYKAMEYEEISRMLVLEEMEEAERLVREFGLKNALVG 335 EYKA+EY+EI+R L L+EM EAE++ ++ G KNALVG Sbjct: 301 EYKAIEYDEIARTLTLDEMREAEKITKKLGFKNALVG 337 >emb|CAA58794.1| (X83963) pyruvate formate lyase activating protein homologue [Thermococcus litoralis] Length = 350 Score = 542 bits (1381), Expect = e-153 Identities = 254/335 (75%), Positives = 288/335 (85%), Gaps = 2/335 (0%) Query: 3 AREALPKYFAILEGDDVPNFMLAKKVEVK--PEDSIEEMWNSHEEGMDKLRENDIKDNPS 60 A++ALP YF+IL G++ PNF +K+VEV ++ +E +W +HEEGM+KLREND+K+NP Sbjct: 16 AKDALPHYFSILSGEEKPNFFYSKQVEVNFGEDEDLESLWETHEEGMEKLRENDLKENPK 75 Query: 61 FSLLDLKAVIANKILESCTLCEIKCRVNRKESVGYCRVKETLIASDFLHYGEEPELVPSY 120 SLLDLKA+IA K+LESC LCE KC NR E +GYCRVKE+L+ASDFLH GEEPELVPSY Sbjct: 76 KSLLDLKALIAYKMLESCELCEFKCHANRFEEIGYCRVKESLVASDFLHIGEEPELVPSY 135 Query: 121 TVFFSGCNFRCVFCQNWDISQFRVGLNYRPEYMALKIERAYRFGAKNVNFVGGEPTPNLP 180 TVFFSGCNFRC FCQNWDISQ+RVGL Y P+ MA+KI AY GAKNVNFVGGEPTPNLP Sbjct: 136 TVFFSGCNFRCAFCQNWDISQYRVGLRYSPKEMAVKIAVAYAEGAKNVNFVGGEPTPNLP 195 Query: 181 FILETLRYVNVPIPVVWNSNMYMSEDSMKLLDGIVDIYLGDFKYGNDRCALKYSKVPRYW 240 FILETL+YV VPIPVVWNSNMYMSE SMKLLDGIVD+YLGDFK+GND ALKYSK PRYW Sbjct: 196 FILETLKYVKVPIPVVWNSNMYMSEKSMKLLDGIVDVYLGDFKWGNDEDALKYSKAPRYW 255 Query: 241 EVVTRNFLLAKNHFRAEFLIRHLVMPNHLECCTRPILKWISENLGKDVRVNVMFQYRPEY 300 E VTRNFLLAKNH++AEFLIRHLVMP HLECCTRPIL+WISENLG+DVRVNVMFQYRPEY Sbjct: 256 ETVTRNFLLAKNHYKAEFLIRHLVMPGHLECCTRPILEWISENLGRDVRVNVMFQYRPEY 315 Query: 301 KAMEYEEISRMLVLEEMEEAERLVREFGLKNALVG 335 +A EY EI R L +EM +A LV+EF LKNALVG Sbjct: 316 RAHEYPEIDRRLTNDEMLKAAELVKEFRLKNALVG 350 >gi|11499543 pyruvate formate-lyase activating enzyme (pflX) [Archaeoglobus fulgidus] >gi|7445242|pir||H69494 pyruvate formate-lyase activating enzyme (pflX) homolog - Archaeoglobus fulgidus >gi|2648591|gb|AAB89304.1| (AE000968) pyruvate formate-lyase activating enzyme (pflX) [Archaeoglobus fulgidus] Length = 332 Score = 326 bits (826), Expect = 3e-88 Identities = 165/333 (49%), Positives = 217/333 (64%), Gaps = 21/333 (6%) Query: 7 LPKYFAILEGDDVPNFMLAKKVEVKPEDSIEEMWNSHE----EGMDKLRENDIKDN-PSF 61 L +Y AI G+ + F +AK++ + +S++EM H E L D+ D P + Sbjct: 4 LERYEAINRGEALAKFQIAKRINAEKAESLDEMLEIHRKLKREFAKVLESGDVPDEKPDY 63 Query: 62 SLLDLKAVIANKILESCTLCEIKCRVNRKESVGYCRVKET-LIASDFLHYGEEPELVPSY 120 S + LK I ++L+SC CE +C VNR E G+C+ T +S+FLH GEEPELVPS+ Sbjct: 64 SFMHLKRDILYEMLKSCNFCERRCGVNRYEKKGFCKCGVTSYFSSEFLHVGEEPELVPSH 123 Query: 121 TVFFSGCNFRCVFCQNWDISQFRVGLNYRPEYMALKIERAY------RFGAKNVNFVGGE 174 T+FF+ C F CVFCQN+DI Y +Y+ E AY R G+KNVNFVGG Sbjct: 124 TIFFNRCTFACVFCQNYDIV-------YEDDYIVDARELAYIIDVRRRQGSKNVNFVGGN 176 Query: 175 PTPNLPFILETLRYVNVPIPVVWNSNMYMSEDSMKLLDGIVDIYLGDFKYGNDRCALKYS 234 P + I E L YV +PVVWNSNMY SE+ ++++ VD++LGDFKYGND CA KYS Sbjct: 177 PDQHAHTIAEILLYVESNVPVVWNSNMYHSEELAEVIEDFVDVWLGDFKYGNDECARKYS 236 Query: 235 KVPRYWEVVTRNFLLAKNHFRAEFLIRHLVMPNHLECCTRPILKWISENLGKDVRVNVMF 294 KV YW+VVTRNFL AK + E LIRHLVMPNH+ECCT PI++W++ENLG+D R N+MF Sbjct: 237 KVANYWKVVTRNFLRAKEN--GELLIRHLVMPNHIECCTEPIVRWVAENLGRDTRFNLMF 294 Query: 295 QYRPEYKAMEYEEISRMLVLEEMEEAERLVREF 327 QY P +KA EY EI+R L EEME+A R+ ++ Sbjct: 295 QYYPTFKAWEYPEIARRLTREEMEKAARIAEKY 327 >pir||H69008 conserved hypothetical protein MTH1069 - Methanobacterium thermoautotrophicum (strain Delta H) >gi|2622167|gb|AAB85558.1| (AE000877) pyruvate formate lyase activating protein [Methanobacterium thermoautotrophicum] Length = 332 Score = 320 bits (811), Expect = 2e-86 Identities = 161/336 (47%), Positives = 218/336 (63%), Gaps = 9/336 (2%) Query: 1 MGAREALPKYFAILEGDDVPNFMLAKKVEVKPEDS--IEEMWNSHEEGMDKLRENDIKDN 58 M ++AL Y ++ +G +FM+A + ++ + E+W HE ++K E Sbjct: 3 MSLKDALHHYLSVRDGRTDASFMVAARTPASFHETSGMSELWREHER-IEKGIECSGACE 61 Query: 59 PSFSLLDLKAVIANKILESCTLCEIKCRVNRKESVGYCRVKETLIASDFLHYGEEPELVP 118 SF LDLK IA +IL C+LC +C VNR G+C V E +AS+FLHYGEE LVP Sbjct: 62 RSF--LDLKIEIAERILGECSLCPWRCGVNRHREQGHCGVMEPRVASEFLHYGEEAPLVP 119 Query: 119 SYTVFFSGCNFRCVFCQNWDISQF-RVGLNYRPEYMALKIERAYRFGAKNVNFVGGEPTP 177 S+T+FFSGC RCVFCQNWDISQ G + + +A IE R GA NVNFVGG+PTP Sbjct: 120 SHTIFFSGCTLRCVFCQNWDISQNPSAGRHIEVDELAALIEERRRMGAANVNFVGGDPTP 179 Query: 178 NLPFILETLRYVNVPIPVVWNSNMYMSEDSMKLLDGIVDIYLGDFKYGNDRCALKYSKVP 237 LP+IL L V++ +PVVWNSNMYM+ +S++L+ G D+YL DFK+GN CA + + Sbjct: 180 ALPYILRVLSRVSISVPVVWNSNMYMTRESLRLIMGAADLYLTDFKFGNSECAERLAGAG 239 Query: 238 RYWEVVTRNFLLAKNHFRAEFLIRHLVMPNHLECCTRPILKWISENLGKDVRVNVMFQYR 297 Y+EVV+RN L+ + +IRHLV+P HLECCT+PI+ W++ENLG D +N+M QYR Sbjct: 240 NYFEVVSRNHLMIAGE---DMIIRHLVLPGHLECCTKPIISWVAENLGTDTVMNIMGQYR 296 Query: 298 PEYKAMEYEEISRMLVLEEMEEAERLVREFGLKNAL 333 P Y+A Y EI+R L +EE+EEA R GL+N + Sbjct: 297 PLYRASAYPEINRPLYIEELEEARRWALSEGLENLI 332 >sp|Q58087|Y674_METJA HYPOTHETICAL PROTEIN MJ0674 >gi|2128025|pir||B64384 hypothetical protein homolog MJ0674 - Methanococcus jannaschii >gi|1591388|gb|AAB98668.1| (U67514) pyruvate formate lyase-activating enzyme isolog (pflX) [Methanococcus jannaschii] Length = 338 Score = 312 bits (790), Expect = 5e-84 Identities = 163/336 (48%), Positives = 223/336 (65%), Gaps = 18/336 (5%) Query: 7 LPKYFAILEGDDVPNFMLAKKVEVKPEDSIE--EMWNSHEEGMDKLRENDIKDNP----S 60 L +Y A+ + F++AK +EV+ +E E+W H + + K+ ++ N Sbjct: 3 LGRYLAVSKDLAPAKFIIAKCIEVEEFKGLELNELWEIHNKVLKKVDFDNFDFNDLEYVK 62 Query: 61 FSLLDLKAVIANKILESCTLCEIKCRVNRKESVGYCRVKETLIASDFLHYGEEPELVPSY 120 +LLDLK IA I ++C CE +C VNR+ G+CR+KE+ +++FLH GEE LVPS+ Sbjct: 63 PNLLDLKVEIAKNIFKNCHFCEHRCYVNRETERGFCRIKESYYSTEFLHLGEERVLVPSH 122 Query: 121 TVFFSGCNFRCVFCQNWDISQFRVG-------LNYRPEYMALKIERAYRFGAKNVNFVGG 173 T+FF GCNF+CVFCQNWDISQ + Y P+ MA KI + R +KNVNFVGG Sbjct: 123 TIFFCGCNFKCVFCQNWDISQVYFDKTIPNHCIPYNPKEMA-KIIKHKRDYSKNVNFVGG 181 Query: 174 EPTPNLPFILETLRYVNVPIPVVWNSNMYMSEDSMKLLDGIVDIYLGDFKYGNDRCALKY 233 +PTP+L IL+TL Y++ IPVVWNSNMY++ + M LL G+VD+YL DFK+GN+ C + Sbjct: 182 DPTPHLLSILKTLSYLDKNIPVVWNSNMYLTVEGMHLLKGVVDVYLTDFKFGNNECGERL 241 Query: 234 SKVPRYWEVVTRNFLLAKNHFRAEFLIRHLVMPNHLECCTRPILKWISENLGKDVRVNVM 293 SKV Y++++ RN LL K+ E +IRHLVMPNHL+CCT I +IS+NL V VNVM Sbjct: 242 SKVKNYFDIIKRNHLLIKDE---EVIIRHLVMPNHLDCCTEKIFDFISKNLDNAV-VNVM 297 Query: 294 FQYRPEYKAMEYEEISRMLVLEEMEEAERLVREFGL 329 FQYRPEYKA EY +I+R L EE+E+A L ++ L Sbjct: 298 FQYRPEYKAKEYPDINRRLTYEEIEKALELAEKYNL 333 >pir||H72634 probable pyruvate formate lyase activating protein APE1535 - Aeropyrum pernix (strain K1) >gi|5105220|dbj|BAA80534.1| (AP000061) 403aa long hypothetical pyruvate formate lyase activating protein [Aeropyrum pernix] Length = 403 Score = 286 bits (723), Expect = 3e-76 Identities = 152/352 (43%), Positives = 214/352 (60%), Gaps = 30/352 (8%) Query: 5 EALPKYFAILEGDDVPNFMLAKKVEV--KPEDSI-----EEMWNSHEEGMDKLR------ 51 E L Y ++ G++ P + + + EV ED I EE+W HE + R Sbjct: 48 EKLGWYSRVMRGEEPPKYRIVRTFEVGFDSEDDIRRALTEELWREHERVAGEFRRLFRSL 107 Query: 52 ------ENDIKDNPSFSLLDLKAVIANKILESCTLCEIKCRVNRKESV-GYCRVK-ETLI 103 E DIKD SL+D+K + ++ C LCE +CRV R +S G CR+ E + Sbjct: 108 REGGSYEGDIKDARP-SLMDVKVELVRRLASPCELCERRCRVERAKSRRGACRLAWEVYV 166 Query: 104 ASDFLHYGEEPELVPSYTVFFSGCNFRCVFCQNWDISQFRVGLNY--RPEYMALKIERAY 161 S FLH GEE LVPS T+F+ GCNF CVFCQNWD+SQ++ + P + +R Sbjct: 167 HSAFLHLGEEAPLVPSGTIFYGGCNFTCVFCQNWDVSQWKARDAHIVTPRRLGEIQDRLA 226 Query: 162 RFGAKNVNFVGGEPTPNLPFILETLRYVNVPIPVVWNSNMYMSEDSMKLLDGIVDIYLGD 221 GA+N+N VGG+P P+L I+E+++Y + P +WNSNMYM+ +++ +L ++DI+L D Sbjct: 227 EAGARNINHVGGDPVPSLHVIVESMKYASHLKPQLWNSNMYMTLEALDILVDLIDIWLPD 286 Query: 222 FKYGNDRCALKYSKVPRYWEVVTRNFLLAKNHFRAEFLIRHLVMPNHLECCTRPILKWIS 281 K+GNDRCA++ S VPRYWEV TRN LLA H + +IRHLVMP H+ECCT+P+L+WI+ Sbjct: 287 LKFGNDRCAMRLSAVPRYWEVTTRNILLAAEH--GDMIIRHLVMPGHVECCTKPVLRWIA 344 Query: 282 ENLGKDVRVNVMFQYRPEYKAM----EYEEISRMLVLEEMEEAERLVREFGL 329 ENLG V VN+M QY P+ + ++ EI+R E+EEA R + GL Sbjct: 345 ENLGDRVLVNIMDQYHPDNLVLRMPHKWPEIARRPSRREIEEAWRYAEQLGL 396 >pir||A70364 conserved hypothetical protein aq_729 - Aquifex aeolicus >gi|2983324|gb|AAC06914.1| (AE000705) hypothetical protein [Aquifex aeolicus] Length = 337 Score = 160 bits (401), Expect = 2e-38 Identities = 104/286 (36%), Positives = 154/286 (53%), Gaps = 24/286 (8%) Query: 64 LDLKAVIANKILESCTLCEIKCRVNRKES--VGYCRVKETLIASDFL-HYGEEPELVP-- 118 L+ + A +LE C +C C VNR E GYC+V + +D+ H+GEE + Sbjct: 53 LEKRVEKALNMLEKCKVCPHTCGVNRLEDDKKGYCKVGRYAVVADYFPHFGEEFPIRGYR 112 Query: 119 -SYTVFFSGCNFRCVFCQNWDISQFRVGLNYRPEYMALKIERAYRFGAKNVNFVGGEPTP 177 S T+FFS CN RCV+CQN+D+S G +PE +A + +G N+N V P+ Sbjct: 113 GSGTIFFSYCNMRCVYCQNYDVSHLGAGRIMKPEDLAEVMLELQDYGCHNINLVS--PSH 170 Query: 178 NLPFILETLRYV---NVPIPVVWNSNMYMSEDSMKLLDGIVDIYLGDFKYGNDRCALKYS 234 +P ILE L + IP+V+N++ + S +++KLLDGIVDIYL D KY N KYS Sbjct: 171 VVPQILEALLIAVERGLRIPIVYNTSSFDSLETLKLLDGIVDIYLADLKYLNKEYGRKYS 230 Query: 235 KVPRYWEV---VTRNFLLAKNHFRAE--------FLIRHLVMPNHLECCTRPILKWISEN 283 KV Y V R + + L+RHLV+PN + T+ +++++ + Sbjct: 231 KVKDYPSVAKEAIREMYRQVGNLEVDERGIAVRGLLVRHLVLPNDIS-TTKEVMEFL-RS 288 Query: 284 LGKDVRVNVMFQYRPEYKAMEYEEISRMLVLEEMEEAERLVREFGL 329 + + VNVM QY P YKA +Y E+SR + EE ++A + F L Sbjct: 289 IDPKLAVNVMKQYHPYYKAWDYPELSRRITEEEYKKALEEAQGFTL 334 >pir||D72416 hypothetical protein TM0109 - Thermotoga maritima (strain MSB8) >gi|4980600|gb|AAD35203.1|AE001697_3 (AE001697) pyruvate formate lyase activating enzyme, putative [Thermotoga maritima] Length = 328 Score = 158 bits (395), Expect = 1e-37 Identities = 94/268 (35%), Positives = 146/268 (54%), Gaps = 18/268 (6%) Query: 75 LESCTLCEIKCRVNRKESVGYCRVK-ETLIASDFLHYGEEPEL---VPSYTVFFSGCNFR 130 LE C LC KC V+R + G C + I++ LH+GEEP + + TVFFSGCN + Sbjct: 28 LEKCELCPRKCGVDRYRTTGICGLPARAKISNAVLHFGEEPPISGKTGAGTVFFSGCNMK 87 Query: 131 CVFCQNWDISQFRVGLNYRPEYMALKIERAYRFGAKNVNFVGGEPTPNLPFILETLRYV- 189 CV+CQN SQ +G E +A + GAK +N V PTP+LPFI+ LR Sbjct: 88 CVYCQNMGFSQKGIGTEVEVEDLAEIFLILQKHGAKTLNLV--TPTPHLPFIISALRIAI 145 Query: 190 --NVPIPVVWNSNMYMSEDSMKLLDGIVDIYLGDFKYGNDRCALKYSKVPRYWEVVTRNF 247 + +P+V+N++ Y + ++LL+G+VDIYL D +Y ++ + KYSK P YW VV + Sbjct: 146 ENGLNLPIVYNTSGYEDPEILRLLEGVVDIYLSDVRYSDNEASKKYSKTPDYWTVVQKAI 205 Query: 248 L-------LAKNHFRAEFLIRHLVMPNHLECCTRPILKWISENLGKDVRVNVMFQYRPEY 300 + + ++R LV+P ++ + I ++S +L + +++M QY P + Sbjct: 206 VEMFRQVGIFDEEKMKGLIVRILVLPGNVVDYSE-IFSFLS-SLSTRIPLSIMNQYIPHF 263 Query: 301 KAMEYEEISRMLVLEEMEEAERLVREFG 328 A ++ EISR L E E+ L +G Sbjct: 264 DAQKFPEISRKLNQNEYEKILELAERYG 291 >pir||E72317 conserved hypothetical protein - Thermotoga maritima (strain MSB8) >gi|4981461|gb|AAD36005.1|AE001756_8 (AE001756) conserved hypothetical protein [Thermotoga maritima] Length = 286 Score = 129 bits (321), Expect = 5e-29 Identities = 92/260 (35%), Positives = 127/260 (48%), Gaps = 29/260 (11%) Query: 66 LKAVIANKILESCTLCEIKCRVNR-KESVGYCRVKETLIASDF-LHYGEEPELVP---SY 120 +K+ + + LE C LC C VNR G C V + + S + H+GEE LV S Sbjct: 1 MKSELFYRKLEKCDLCPRNCGVNRLMGEKGACGVANSPVVSSWGPHFGEERILVGRGGSG 60 Query: 121 TVFFSGCNFRCVFCQNWDISQFRVGLNYRPEYMALKIERAYRFGAKNVNFVGGEPTPNLP 180 TVFF+ CN +CV+CQN++ISQ +G E + G +N+N V PT +P Sbjct: 61 TVFFTYCNLKCVYCQNYEISQLGIGKEITVEDLLRIFTELQDMGVENLNLV--TPTHQIP 118 Query: 181 FILETLRYVNVPIPVVWNSNMYMSEDSMKLLDGIVDIYLGDFKYGNDRCALKYSKVPRY- 239 FI++ + IPVV+N Y S D++ L+G VDIY+ DFKY + K S V Y Sbjct: 119 FIVDAFERMEKEIPVVYNCGGYESVDTIISLEGFVDIYMPDFKYSDPELGEKLSGVKDYP 178 Query: 240 --------------WEVVTRNFLLAKNHFRAEFLIRHLVMPNHLECCTRPILKWISENLG 285 E V RN ++ K L+RHLV+P LE I + L Sbjct: 179 RFALEALKVMIDQMGEPVIRNGVMKKG-----VLVRHLVLPGFLEDSFGVI--DLLSTLE 231 Query: 286 KDVRVNVMFQYRPEYKAMEY 305 VN+M Q+ P Y+A EY Sbjct: 232 PKPLVNIMAQFYPAYRAKEY 251 >gb|AAG23565.1|AF244616_1 (AF244616) hypothetical protein [Carboxydothermus hydrogenoformans] Length = 171 Score = 99.5 bits (244), Expect = 5e-20 Identities = 61/172 (35%), Positives = 97/172 (55%), Gaps = 9/172 (5%) Query: 78 CTLCEIKCRVNRKESV-GYCRVKET-LIASDFLHYGEEPELVPSYT--VFFSGCNFRCVF 133 CTLC C+VNR G+C ++AS H+GEE ++ +F+S C +CV Sbjct: 2 CTLCPHNCQVNRSRGERGFCGGGYLPVVASYGPHFGEERLXWGTWFRHIFYSHCTXKCVC 61 Query: 134 CQNWDISQFRVGLNYRPEYMALKIERAYRFGAKNVNFVGGEPTPNLPFILETLRYV---N 190 QN +ISQ G RP+ +A + R G N+N V PT +P I++ + Sbjct: 62 SQNSEISQXGEGEELRPQDLAKIMLILQRRGCHNINXVS--PTHYVPAIVKAVLIAAEEG 119 Query: 191 VPIPVVWNSNMYMSEDSMKLLDGIVDIYLGDFKYGNDRCALKYSKVPRYWEV 242 + IP+V+N++ Y + ++++LL+GIVDIY+ D K+G+D KY+K P Y+ + Sbjct: 120 LKIPLVYNTSGYENVETLRLLEGIVDIYMPDIKFGDDEIGKKYAKAPGYFAI 171 >pir||G75119 pyruvate formate-lyase activating enzyme related protein (act-like) PAB1859 - Pyrococcus abyssi (strain Orsay) >gi|5458183|emb|CAB49672.1| (AJ248285) pyruvate formate-lyase activating enzyme related protein (act-like) [Pyrococcus abyssi] Length = 348 Score = 79.6 bits (193), Expect = 5e-14 Identities = 68/279 (24%), Positives = 124/279 (44%), Gaps = 28/279 (10%) Query: 78 CTLCEIKCRVNRKESVGYCRVKETLIASDF-LHYGEEPELVP----------------SY 120 C LC ++C ++ + G C+++ + L+YG+ + ++ Sbjct: 17 CELCPLRCIIDEGKR-GSCKIRINKGGKLYTLNYGKVSSIALDPIEKKPLFHFWPGSCAF 75 Query: 121 TVFFSGCNFRCVFCQNWDISQFRVGLNYRPEYMALKIER-AYRFGAKNVNFVGGEPTPNL 179 ++ GCN C CQNW+ISQ Y + I R A + +++ + EPT Sbjct: 76 SIGTVGCNMHCKHCQNWEISQADENFPYLQDATPEAIVRLAKHYECESIAYTYNEPTIWY 135 Query: 180 PFILETLRYVNVP-IPVVWNSNMYMSEDSMKLLDGIVDIYLGDFKYGNDRCALKYSKVPR 238 F+L+T + + + +N Y++E+ + L +D D K +DR +K + VP Sbjct: 136 EFVLDTAKLAKKEGLNNILVTNGYINEEPFRELAPYIDAMNIDIKAFDDRFYMKIASVPN 195 Query: 239 YWEVVTRNFLLAKNHFRAEFLIRHLVMP--NHLECCTRPILKWISENLGKDVRVNVMFQY 296 E R +AK F + +L++P N E R +W+ + LG D V+ ++ Sbjct: 196 -GEPSRRIAKIAKKEFGIHVELTYLIIPTLNDKEEEIRAFARWVVDELGSDTPVH-FSRF 253 Query: 297 RPEYKAMEYEEISRMLVLEEMEEAERLVREFGLKNALVG 335 P YK + +E +E+A ++ +E GLK +G Sbjct: 254 FPHYKLLNLPPTP----IETLEKAYKIAKEEGLKFVYLG 288 >pir||A71012 hypothetical protein PH1391 - Pyrococcus horikoshii >gi|3257814|dbj|BAA30497.1| (AP000006) 348aa long hypothetical protein [Pyrococcus horikoshii] Length = 348 Score = 79.2 bits (192), Expect = 6e-14 Identities = 69/282 (24%), Positives = 126/282 (44%), Gaps = 34/282 (12%) Query: 78 CTLCEIKCRVNRKESVGYCRVKETLIASDF-LHYGEEPELVP----------------SY 120 C LC ++C ++ + G C+++ + L+YG+ + + Sbjct: 17 CELCPLRCIIDEGKR-GSCKIRINKGGKLYTLNYGKVSSIALDPIEKKPLFHFWPGSCAL 75 Query: 121 TVFFSGCNFRCVFCQNWDISQFRVGLNY----RPEYMALKIERAYRFGAKNVNFVGGEPT 176 ++ GCN C CQNW+ISQ Y PE + + A + +++ + EPT Sbjct: 76 SIGTVGCNMHCKHCQNWEISQADESFPYLQDATPEAI---VSLAKHYECESIAYTYNEPT 132 Query: 177 PNLPFILETLRYVNVP-IPVVWNSNMYMSEDSMKLLDGIVDIYLGDFKYGNDRCALKYSK 235 F+L+T + + + +N Y++E+ + L +D D K +D+ +K + Sbjct: 133 IWYEFVLDTAKLARKEGLNNILVTNGYINEEPFRELAPYIDAMNIDIKAFDDKFYMKIAS 192 Query: 236 VPRYWEVVTRNFLLAKNHFRAEFLIRHLVMP--NHLECCTRPILKWISENLGKDVRVNVM 293 VP E R +AK F+ + +L++P N E R +W+ ENLG D V+ Sbjct: 193 VPS-GEPSRRIAEIAKKEFKIHVELTYLIIPTLNDKEEEIREFARWVVENLGDDTPVH-F 250 Query: 294 FQYRPEYKAMEYEEISRMLVLEEMEEAERLVREFGLKNALVG 335 ++ P YK + +E +E+A ++ +E GLK +G Sbjct: 251 SRFFPHYKLLNLPPTP----IETLEKAYKIAKEEGLKFVYLG 288 >pir||F69230 pyruvate formate-lyase activating enzyme related protein - Methanobacterium thermoautotrophicum (strain Delta H) >gi|2622075|gb|AAB85472.1| (AE000871) pyruvate formate-lyase activating enzyme related protein [Methanobacterium thermoautotrophicum] Length = 304 Score = 72.2 bits (174), Expect = 8e-12 Identities = 71/261 (27%), Positives = 118/261 (45%), Gaps = 22/261 (8%) Query: 78 CTLCEIKCRVNRKESV-GYCRVKETLIASDFLHYGEEPELVPSYTVFFSGCNFRCVFCQN 136 C LCE +C NR + G C ET IA Y E + SY+V F C FRC +C Sbjct: 7 CMLCEWRCGANRSDGERGVCGASETEIA-----YTSISETLRSYSVTFLCCPFRCAYCNA 61 Query: 137 WDISQF-RVGLNYRPEYMALKIERAYRFGAK------NVNFVGGEPTPNLPFILETLRYV 189 + ISQ+ G YR A ++ K N++F GGEP+ + P+I E +R V Sbjct: 62 YRISQYPHSGWIYRGHVEAEELADEAITAIKSHERISNISFTGGEPSIHTPYIEELVRRV 121 Query: 190 --NVPIPVVWNSNMYMSEDSMKLLDGIVDIYLGDFKYGNDRCALKYSKVPRYWEVVTRNF 247 + + V+ +N + + +++ L G+ ++ + K +D + P E V RN Sbjct: 122 REEIDVSVILATNGFSTPGTLRRLIGLTSLFSFEIKALSDELHRNLTGAPA--EPVLRNA 179 Query: 248 LLAKNHFRAEF-LIRHLVMPNHLECCTRPILKWISENLGKDVRVNVMFQYRPEYKAMEYE 306 F + + R +V+P + R I +I+ ++ ++ + +RP + + Sbjct: 180 AHLAGRFPDKIRVFRTVVIPGINDHEIREIAAFIA-SIDPEIPYR-LIGFRPNFMLYYHR 237 Query: 307 EISRMLVLEEMEE--AERLVR 325 R L+ +EE AE L R Sbjct: 238 GPGRELMERLVEECRAEGLER 258 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.323 0.140 0.429 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 132595143 Number of Sequences: 2977 Number of extensions: 5863030 Number of successful extensions: 11836 Number of sequences better than 1.0e-10: 14 Number of HSP's better than 0.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 7 Number of HSP's that attempted gapping in prelim test: 11791 Number of HSP's gapped (non-prelim): 14 length of query: 335 length of database: 189,106,746 effective HSP length: 52 effective length of query: 283 effective length of database: 157,985,422 effective search space: 44709874426 effective search space used: 44709874426 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.9 bits) S2: 165 (68.7 bits)