BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0750 (asnB) DE:asparagine synthetase, putative
(479 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||G75092 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 950 0.0
pir||G71050 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 763 0.0
sp|Q58516|ASNH_METJA PUTATIVE ASPARAGINE SYNTHETASE [GLUTAMINE-H... 272 5e-72
gb|AAB99117.1| (U67554) asparagine synthetase (asnB) [Methanococ... 272 5e-72
pir||H69153 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 261 2e-68
gb|AAB84920.1| (AE000825) asparagine synthetase [Methanobacteriu... 261 2e-68
gi|11499034 asparagine synthetase (asnB) [Archaeoglobus fulgidus... 160 4e-38
pir||H82255 asparagine synthetase B, glutamine-hydrolyzing VC099... 149 6e-35
dbj|BAA89376.1| (AB025342) ORF2 [Moritella marina] 148 1e-34
sp|P22106|ASNB_ECOLI ASPARAGINE SYNTHETASE B [GLUTAMINE-HYDROLYZ... 145 1e-33
pir||T16625 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 143 3e-33
gb|AAF02776.1|AF190729_1 (AF190729) asparagine synthetase [Helia... 142 1e-32
pdb|1CT9|A Chain A, Crystal Structure Of Asparagine Synthetase B... 141 2e-32
sp|P49092|ASN1_LOTJA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 141 2e-32
emb|CAA08913.1| (AJ009952) asparagine synthetase type II [Phaseo... 139 5e-32
pir||T08846 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 139 6e-32
gb|AAC25799.1| (AF022973) Contains similarity to Pfam domain: PF... 138 1e-31
sp|P19252|ASN2_PEA ASPARAGINE SYNTHETASE, ROOT [GLUTAMINE-HYDROL... 138 1e-31
gb|AAF02775.1|AF190728_1 (AF190728) asparagine synthetase [Helia... 138 1e-31
gb|AAC72836.1| (AF095452) asparagine synthetase [Arabidopsis tha... 138 2e-31
sp|O24338|ASNS_SANAU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 138 2e-31
pir||T50812 asparagine synthetase ASN3 - Arabidopsis thaliana >g... 137 2e-31
sp|P54420|ASNB_BACSU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 135 9e-31
gb|AAB95197.1| (U84118) asparagine synthetase [Aedes aegypti] 135 9e-31
pir||T18441 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 135 9e-31
pir||JW0071 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 135 1e-30
gb|AAC72837.1| (AF095453) asparagine synthetase [Arabidopsis tha... 134 2e-30
dbj|BAA97313.1| (AB019236) asparagine synthetase [Arabidopsis th... 134 2e-30
sp|P31752|ASNS_ASPOF ASPARAGINE SYNTHASE [GLUTAMINE-HYDROLYZING]... 134 3e-30
emb|CAA67889.1| (X99552) asparagine synthetase [Asparagus offici... 134 3e-30
dbj|BAA96251.1| (AB035247) asparagine synthetase [Astragalus sin... 134 3e-30
gb|AAB81011.1| (U89923) asparagine synthetase [Medicago sativa] 133 5e-30
gb|AAB48058.1| (L40327) asparagine synthetase [Medicago sativa] 133 5e-30
sp|O24661|ASNS_TRIVS ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 132 6e-30
dbj|BAA96252.1| (AB035248) asparagine synthetase [Astragalus sin... 132 6e-30
sp|P49078|ASNS_ARATH ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 132 8e-30
emb|CAB57292.1| (AJ133522) asparagine synthetase (type-I) [Phase... 132 1e-29
gb|AAF74755.1| (AF263432) asparagine synthetase [Helianthus annuus] 132 1e-29
gi|11421677 asparagine synthetase [Homo sapiens] >gi|3341715|gb|... 131 1e-29
gb|AAC16325.1| (AF061740) asparagine synthetase [Elaeagnus umbel... 131 1e-29
prf||2207183A Asn synthetase [Rattus norvegicus] 131 2e-29
sp|P49088|ASNS_RAT ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]... 130 3e-29
gi|4502259 asparagine synthetase [Homo sapiens] >gi|114266|sp|P0... 130 3e-29
sp|P49093|ASN2_LOTJA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 130 3e-29
sp|Q43011|ASNS_ORYSA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 130 3e-29
gi|6321563 asparagine synthetase; Asn2p [Saccharomyces cerevisia... 130 4e-29
sp|P19891|ASNS_CRIGR ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 129 5e-29
emb|CAA96526.1| (Z72354) asparagine synthetase [Vicia faba] 129 7e-29
pir||T39308 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 129 7e-29
pir||AJHUN2 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 129 9e-29
sp|P19251|ASN1_PEA ASPARAGINE SYNTHETASE, NODULE [GLUTAMINE-HYDR... 129 9e-29
sp|Q61024|ASNS_MOUSE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 128 2e-28
sp|P49091|ASNS_BRAOL ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 128 2e-28
dbj|BAB17726.1| (AB050900) asparagine synthetase [Raphanus sativus] 128 2e-28
sp|P49094|ASNS_MAIZE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 127 3e-28
gb|AAC49614.1| (U77679) asparagine synthetase 1 [Glycine max] 127 4e-28
pir||D82846 asparagine synthase B XF0118 [imported] - Xylella fa... 125 8e-28
gi|6325403 asparagine synthetase; Asn1p [Saccharomyces cerevisia... 125 1e-27
sp|P17714|ASNS_MESAU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 123 3e-27
gb|AAG19311.1| (AE005026) asparagine synthetase; AsnA [Halobacte... 120 3e-26
emb|CAB58327.1| (AL121855) putative amidotransferase [Streptomyc... 119 6e-26
dbj|BAA89484.1| (AB029550) LtsA [Corynebacterium glutamicum] 118 2e-25
pir||T50028 asparagine synthetase (ASN3) - Arabidopsis thaliana ... 104 3e-21
dbj|BAB05227.1| (AP001512) asparagine synthetase [Bacillus halod... 104 3e-21
emb|CAA07652.1| (AJ007747) putative glutamine amidotransferase [... 103 4e-21
emb|CAC11460.1| (AL445063) asparagine synthetase related protein... 102 9e-21
sp|Q10374|ASNH_MYCTU PUTATIVE ASPARAGINE SYNTHETASE [GLUTAMINE-H... 101 1e-20
pir||F75095 probable asparagine synthase (glutamine-hydrolyzing)... 100 3e-20
sp|O05272|ASNO_BACSU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 99 1e-19
pir||F69837 asparagine synthase homolog yisO - Bacillus subtilis... 99 1e-19
gi|6323542 Yml096wp [Saccharomyces cerevisiae] >gi|2494766|sp|Q0... 98 2e-19
pir||A83638 probable glutamine amidotransferase PA0051 [imported... 89 1e-16
pir||B75212 amidophosphoribosyltransferase (EC 2.4.2.14) PAB0151... 86 7e-16
dbj|BAB12569.1| (AB039379) asparagine synthase homolog [Streptom... 86 1e-15
pir||JQ2245 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 84 4e-15
pir||C72508 hypothetical protein APE2041 - Aeropyrum pernix (str... 81 2e-14
emb|CAC12366.1| (AL445067) probable amidophosphoribosyltransfera... 81 2e-14
gb|AAD38229.1| (U17224) CarA [Pectobacterium carotovorum] 79 1e-13
pir||A72277 amidophosphoribosyltransferase (EC 2.4.2.14) TM1247 ... 78 3e-13
sp|O26742|PUR1_METTH AMIDOPHOSPHORIBOSYLTRANSFERASE PRECURSOR (G... 77 5e-13
pir||A70401 amidophosphoribosyltransferase (EC 2.4.2.14) purF [s... 77 5e-13
pir||T40508 probable asparagine synthase - fission yeast (Schiz... 77 5e-13
sp|O57979|PUR1_PYRHO AMIDOPHOSPHORIBOSYLTRANSFERASE PRECURSOR (G... 76 1e-12
sp|Q57657|PUR1_METJA PROBABLE AMIDOPHOSPHORIBOSYLTRANSFERASE (GL... 75 1e-12
pir||A75545 amidophosphoribosyltransferase (EC 2.4.2.14) DR0220 ... 75 2e-12
pir||T42010 probable asparagine synthase (glutamine-hydrolyzing)... 71 3e-11
>pir||G75092 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) PAB0750
[similarity] - Pyrococcus abyssi (strain Orsay)
>gi|5458556|emb|CAB50044.1| (AJ248286) asparagine
synthetase, putative [Pyrococcus abyssi]
Length = 479
Score = 950 bits (2429), Expect = 0.0
Identities = 479/479 (100%), Positives = 479/479 (100%)
Query: 1 MCLIAGGFNVSREDAIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
MCLIAGGFNVSREDAIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC
Sbjct: 1 MCLIAGGFNVSREDAIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
Query: 61 RLAMTGSKSYTQPFYNEIILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLV 120
RLAMTGSKSYTQPFYNEIILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLV
Sbjct: 61 RLAMTGSKSYTQPFYNEIILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLV 120
Query: 121 FDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLFRDPLGVRPLYYSSRGHFASEKKVLW 180
FDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLFRDPLGVRPLYYSSRGHFASEKKVLW
Sbjct: 121 FDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLFRDPLGVRPLYYSSRGHFASEKKVLW 180
Query: 181 GLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFNGLQNLLIDSVKVRASKKV 240
GLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFNGLQNLLIDSVKVRASKKV
Sbjct: 181 GLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFNGLQNLLIDSVKVRASKKV 240
Query: 241 GVLFSGGVDSSLIALIASRYSDVILYTSGTEDSKDVEWARRVAEELGLKLRESLFSREDI 300
GVLFSGGVDSSLIALIASRYSDVILYTSGTEDSKDVEWARRVAEELGLKLRESLFSREDI
Sbjct: 241 GVLFSGGVDSSLIALIASRYSDVILYTSGTEDSKDVEWARRVAEELGLKLRESLFSREDI 300
Query: 301 ENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGYAKYLQNP 360
ENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGYAKYLQNP
Sbjct: 301 ENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGYAKYLQNP 360
Query: 361 GLMLKDVEELGERNLARDDKVAMLNGVETRYPYLDLPFAILALNVPLELKIRDGKRKFIL 420
GLMLKDVEELGERNLARDDKVAMLNGVETRYPYLDLPFAILALNVPLELKIRDGKRKFIL
Sbjct: 361 GLMLKDVEELGERNLARDDKVAMLNGVETRYPYLDLPFAILALNVPLELKIRDGKRKFIL 420
Query: 421 REIAKSLGLPEWVAEREKKAAQYGSNAQKILEKIAKSRGVKLKGLADILFSKVFHGIQN 479
REIAKSLGLPEWVAEREKKAAQYGSNAQKILEKIAKSRGVKLKGLADILFSKVFHGIQN
Sbjct: 421 REIAKSLGLPEWVAEREKKAAQYGSNAQKILEKIAKSRGVKLKGLADILFSKVFHGIQN 479
>pir||G71050 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) PH1102
[similarity] - Pyrococcus horikoshii
>gi|3257518|dbj|BAA30201.1| (AP000005) 480aa long
hypothetical asparagine synthetase [Pyrococcus
horikoshii]
Length = 480
Score = 763 bits (1949), Expect = 0.0
Identities = 366/479 (76%), Positives = 427/479 (88%)
Query: 1 MCLIAGGFNVSREDAIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
MCLIAGGF + ++ I+II LGKHRGPDSFG WSER+++KS +FS + E++GG+ LIQC
Sbjct: 1 MCLIAGGFKLYTDEIIRIIELGKHRGPDSFGVWSEREVIKSSDFSALEEVKGGDKVLIQC 60
Query: 61 RLAMTGSKSYTQPFYNEIILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLV 120
RLAMTGSK YTQPFYNEI LVHNGEIYNHE LR YL+ERG++FE+DVDSEVILRLLEYL+
Sbjct: 61 RLAMTGSKEYTQPFYNEIALVHNGEIYNHEKLRAYLVERGISFESDVDSEVILRLLEYLL 120
Query: 121 FDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLFRDPLGVRPLYYSSRGHFASEKKVLW 180
FDR L AIK M MLRGDYAVAF +NGKFYLFRDPLG+RPLYYSS+GHF SEKKVLW
Sbjct: 121 FDRNLSPWNAIKKLMWMLRGDYAVAFHYNGKFYLFRDPLGIRPLYYSSQGHFGSEKKVLW 180
Query: 181 GLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFNGLQNLLIDSVKVRASKKV 240
GLGE+A PV+PGE V ISK GI RVR ++V +I++ G +YF+GL+NLLIDSVK+R+SKK+
Sbjct: 181 GLGEEATPVMPGEAVEISKFGINRVRLYDVTAIKSFGSEYFDGLRNLLIDSVKIRSSKKI 240
Query: 241 GVLFSGGVDSSLIALIASRYSDVILYTSGTEDSKDVEWARRVAEELGLKLRESLFSREDI 300
G+LFSGG+DS+LIA IASRYS+V LY SGTEDSKDVEWAR+V++ELGLKL+E +FS+E++
Sbjct: 241 GILFSGGLDSTLIAFIASRYSNVTLYVSGTEDSKDVEWARKVSQELGLKLKEYIFSKEEV 300
Query: 301 ENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGYAKYLQNP 360
E E++RIMFAIEEPNPMNL+IAIPLYFSTK AREDGVK+LLSGQGADELFGGYAKYL+NP
Sbjct: 301 EKELKRIMFAIEEPNPMNLSIAIPLYFSTKLAREDGVKILLSGQGADELFGGYAKYLENP 360
Query: 361 GLMLKDVEELGERNLARDDKVAMLNGVETRYPYLDLPFAILALNVPLELKIRDGKRKFIL 420
LM+ DV LGERNLARDDKV+M+NGVE RYPYLDLPFA+LALN PL+ KI+DGKRK IL
Sbjct: 361 NLMISDVANLGERNLARDDKVSMINGVEVRYPYLDLPFAVLALNTPLQHKIKDGKRKIIL 420
Query: 421 REIAKSLGLPEWVAEREKKAAQYGSNAQKILEKIAKSRGVKLKGLADILFSKVFHGIQN 479
REIAKSLGLPEW+AEREKKAAQYGSNAQKILEKIAKSRG++LK LA +LFS+VF GI N
Sbjct: 421 REIAKSLGLPEWIAEREKKAAQYGSNAQKILEKIAKSRGIRLKELASLLFSEVFRGIHN 479
>sp|Q58516|ASNH_METJA PUTATIVE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]
>gi|11272681|pir||C64439 asparagine synthase
(glutamine-hydrolyzing) (EC 6.3.5.4) MJ1116 [similarity]
- Methanococcus jannaschii
Length = 541
Score = 272 bits (689), Expect = 5e-72
Identities = 198/549 (36%), Positives = 293/549 (53%), Gaps = 99/549 (18%)
Query: 1 MCLIAGGF----NVSREDAIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEG---G 53
MC I+G +S + +I ++ + KHRG D+ G + +++ ++F DV ++E G
Sbjct: 1 MCSISGIIVKDNQISAKYSIDMMKILKHRGRDNSGLLLDDEVIYFNDFEDVEDLEEEMIG 60
Query: 54 EIALIQCRLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSE 110
++L RLA+ G + QP NE I LV NGEIYN+ LR+YL ++ F TD D+E
Sbjct: 61 NLSLAHNRLAIVG-RYGVQPIPNEDETIWLVCNGEIYNYIELREYL-KQNHEFRTDSDNE 118
Query: 111 VILRLLEYLVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGK--FYLFRDPLGVRPLYYSS 168
VI+ L E + K+EE L GDYA A + K L RD GV+PL+Y
Sbjct: 119 VIIHLYE------EEKLEE--------LDGDYAFAIYDKSKNVVRLARDMFGVKPLFYVD 164
Query: 169 RGH---FASEKKVLW--------------GLGEDAEPVLPGE--IVSISKHGITRVRGFN 209
R FASE+K LW L + + P I + + + GF
Sbjct: 165 RDKYFAFASERKALWHLLINIDGCERDLDELNSKIKTLKPNSQLIYYLDDNRFEIIEGFK 224
Query: 210 VFSIR-------TQGKDYFN-GLQNLLIDSVKVRASKKVGVLFSGGVDSSLIALIASRYS 261
+ + K+Y + L+N ++ +VR KVG++ SGGVDSSLIA +AS Y
Sbjct: 225 KLELNYMKERSYEEAKEYLDRALKNSVLK--RVRGLDKVGIICSGGVDSSLIAKLASLYC 282
Query: 262 DVILYTSGTEDSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAI 321
+VILY GTE+S+D+ +A R+A++L LKLR+ + S E+ E + ++ AI+E + M + +
Sbjct: 283 EVILYAVGTENSEDLIYAERLAKDLNLKLRKKIISEEEYEEYVFKVAKAIDEVDLMKIGV 342
Query: 322 AIPLYFSTKRAREDGVKVLLSGQGADELFGGYAKY---------LQNPGLMLKDVEELGE 372
IP+Y +++ A EDG+KV+LSGQGADELFGGYA++ + +LKDV L +
Sbjct: 343 GIPIYVASEMANEDGLKVVLSGQGADELFGGYARHERIYRERGEEELKKELLKDVYNLYK 402
Query: 373 RNLARDDKVAMLNGVETRYPYLDLPFAILALNVPLELKIRD------------------- 413
NL RDD M NGVE R P+LD +AL++P+E K+ +
Sbjct: 403 VNLERDDHCTMANGVELRVPFLDEEVVEIALSIPIEYKMSELSNRPYAESNISLKSEPIN 462
Query: 414 -------------GKRKFILREIAKSLGLPEWVAEREKKAAQYGSNAQKILEKIAKSRGV 460
RK ILR++A S LP+++A R KKAAQYGS +K++ K+AK G
Sbjct: 463 GLKNTNLNIKCVRSVRKKILRDVA-SQYLPDYIAYRPKKAAQYGSGGEKMIYKVAKKYGF 521
Query: 461 KLKGLADIL 469
K + + L
Sbjct: 522 SKKRINEFL 530
>gb|AAB99117.1| (U67554) asparagine synthetase (asnB) [Methanococcus jannaschii]
Length = 544
Score = 272 bits (689), Expect = 5e-72
Identities = 198/549 (36%), Positives = 293/549 (53%), Gaps = 99/549 (18%)
Query: 1 MCLIAGGF----NVSREDAIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEG---G 53
MC I+G +S + +I ++ + KHRG D+ G + +++ ++F DV ++E G
Sbjct: 4 MCSISGIIVKDNQISAKYSIDMMKILKHRGRDNSGLLLDDEVIYFNDFEDVEDLEEEMIG 63
Query: 54 EIALIQCRLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSE 110
++L RLA+ G + QP NE I LV NGEIYN+ LR+YL ++ F TD D+E
Sbjct: 64 NLSLAHNRLAIVG-RYGVQPIPNEDETIWLVCNGEIYNYIELREYL-KQNHEFRTDSDNE 121
Query: 111 VILRLLEYLVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGK--FYLFRDPLGVRPLYYSS 168
VI+ L E + K+EE L GDYA A + K L RD GV+PL+Y
Sbjct: 122 VIIHLYE------EEKLEE--------LDGDYAFAIYDKSKNVVRLARDMFGVKPLFYVD 167
Query: 169 RGH---FASEKKVLW--------------GLGEDAEPVLPGE--IVSISKHGITRVRGFN 209
R FASE+K LW L + + P I + + + GF
Sbjct: 168 RDKYFAFASERKALWHLLINIDGCERDLDELNSKIKTLKPNSQLIYYLDDNRFEIIEGFK 227
Query: 210 VFSIR-------TQGKDYFN-GLQNLLIDSVKVRASKKVGVLFSGGVDSSLIALIASRYS 261
+ + K+Y + L+N ++ +VR KVG++ SGGVDSSLIA +AS Y
Sbjct: 228 KLELNYMKERSYEEAKEYLDRALKNSVLK--RVRGLDKVGIICSGGVDSSLIAKLASLYC 285
Query: 262 DVILYTSGTEDSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAI 321
+VILY GTE+S+D+ +A R+A++L LKLR+ + S E+ E + ++ AI+E + M + +
Sbjct: 286 EVILYAVGTENSEDLIYAERLAKDLNLKLRKKIISEEEYEEYVFKVAKAIDEVDLMKIGV 345
Query: 322 AIPLYFSTKRAREDGVKVLLSGQGADELFGGYAKY---------LQNPGLMLKDVEELGE 372
IP+Y +++ A EDG+KV+LSGQGADELFGGYA++ + +LKDV L +
Sbjct: 346 GIPIYVASEMANEDGLKVVLSGQGADELFGGYARHERIYRERGEEELKKELLKDVYNLYK 405
Query: 373 RNLARDDKVAMLNGVETRYPYLDLPFAILALNVPLELKIRD------------------- 413
NL RDD M NGVE R P+LD +AL++P+E K+ +
Sbjct: 406 VNLERDDHCTMANGVELRVPFLDEEVVEIALSIPIEYKMSELSNRPYAESNISLKSEPIN 465
Query: 414 -------------GKRKFILREIAKSLGLPEWVAEREKKAAQYGSNAQKILEKIAKSRGV 460
RK ILR++A S LP+++A R KKAAQYGS +K++ K+AK G
Sbjct: 466 GLKNTNLNIKCVRSVRKKILRDVA-SQYLPDYIAYRPKKAAQYGSGGEKMIYKVAKKYGF 524
Query: 461 KLKGLADIL 469
K + + L
Sbjct: 525 SKKRINEFL 533
>pir||H69153 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) MTH414
[similarity] - Methanobacterium thermoautotrophicum
(strain Delta H)
Length = 466
Score = 261 bits (659), Expect = 2e-68
Identities = 177/475 (37%), Positives = 265/475 (55%), Gaps = 48/475 (10%)
Query: 1 MCLIAGGFNVSREDA-IKI---INLGKHRGPDSFGFWSE-RQMLKSDNFSDVSEI-EGGE 54
MC IAG +DA +K+ +++ +HRGPD+ G + + R ++++ DV E + E
Sbjct: 1 MCAIAG---FRGDDAPVKVQEMLDVLRHRGPDATGIYHDSRIVIRTATGEDVKEAPQSAE 57
Query: 55 IALIQCRLAMTGSKSYTQPFYNEIILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILR 114
IA+ L++ G QP + +LV NGEIYN E L T DSE+IL
Sbjct: 58 IAVGHNLLSIVGGP---QPVSGDGVLVFNGEIYNQEELNW----------TGSDSELILD 104
Query: 115 LLEYLVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLFRDPLGVRPLYYSSRGHFAS 174
L+E D +E+A++ ++ + GDYA A+ +DP+GV+PLY+S FAS
Sbjct: 105 LIEGHGGD----LEDALRFTVNKIDGDYAFAYTDGENLASRQDPVGVKPLYHSGEA-FAS 159
Query: 175 EKKVLWGLG-EDAEPVLPGEI----VSISKHGITRVRGFNVFSIRTQGKDYFNGLQNLLI 229
EKK LW +G + E + PG V + G+ R + + ++ L++ L
Sbjct: 160 EKKALWRIGLRNVETLPPGHAMINGVKVKLRGLPRAEPSDA-----EPEELRENLKSALR 214
Query: 230 DSV--KVRASKKVGVLFSGGVDSSLIALIASRYSDVILYTSGTEDSKDVEWARRVAEELG 287
+SV +VR + ++FSGGVDS+L+A++ + DV LYT G S D ++A R A +LG
Sbjct: 215 ESVERRVRGLDEAALVFSGGVDSTLLAVLLDEHIDVRLYTVGLPGSSDPQFASRAAADLG 274
Query: 288 LKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGAD 347
++L + + I + ++ AIEE N M + +A+PLY +++ A DG +V+ SGQGAD
Sbjct: 275 MELEVLEVTEDTIREALPHVLGAIEEYNLMKIGVAMPLYLASEAASADGYRVMFSGQGAD 334
Query: 348 ELFGGYAKYL------QNPGLMLKDVEELGERNLARDDKVAMLNGVETRYPYLDLPFAIL 401
ELF GY +Y + + D+E + NL RDD V M N VE R P+LDL +
Sbjct: 335 ELFAGYHRYRRLLAEGRLEDALRHDLENIHHVNLERDDAVTMANSVELRVPFLDLQLIGV 394
Query: 402 ALNVPLELKIR---DGKRKFILREIAKSLGLPEWVAEREKKAAQYGSNAQKILEK 453
AL +P +LKIR D RK ILRE A +G+PE++A R KKAAQYGS K+L +
Sbjct: 395 ALTIPADLKIRGPEDELRKRILREAALEMGVPEYIAMRPKKAAQYGSGIDKVLRR 449
>gb|AAB84920.1| (AE000825) asparagine synthetase [Methanobacterium
thermoautotrophicum]
Length = 469
Score = 261 bits (659), Expect = 2e-68
Identities = 177/475 (37%), Positives = 265/475 (55%), Gaps = 48/475 (10%)
Query: 1 MCLIAGGFNVSREDA-IKI---INLGKHRGPDSFGFWSE-RQMLKSDNFSDVSEI-EGGE 54
MC IAG +DA +K+ +++ +HRGPD+ G + + R ++++ DV E + E
Sbjct: 4 MCAIAG---FRGDDAPVKVQEMLDVLRHRGPDATGIYHDSRIVIRTATGEDVKEAPQSAE 60
Query: 55 IALIQCRLAMTGSKSYTQPFYNEIILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILR 114
IA+ L++ G QP + +LV NGEIYN E L T DSE+IL
Sbjct: 61 IAVGHNLLSIVGGP---QPVSGDGVLVFNGEIYNQEELNW----------TGSDSELILD 107
Query: 115 LLEYLVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLFRDPLGVRPLYYSSRGHFAS 174
L+E D +E+A++ ++ + GDYA A+ +DP+GV+PLY+S FAS
Sbjct: 108 LIEGHGGD----LEDALRFTVNKIDGDYAFAYTDGENLASRQDPVGVKPLYHSGEA-FAS 162
Query: 175 EKKVLWGLG-EDAEPVLPGEI----VSISKHGITRVRGFNVFSIRTQGKDYFNGLQNLLI 229
EKK LW +G + E + PG V + G+ R + + ++ L++ L
Sbjct: 163 EKKALWRIGLRNVETLPPGHAMINGVKVKLRGLPRAEPSDA-----EPEELRENLKSALR 217
Query: 230 DSV--KVRASKKVGVLFSGGVDSSLIALIASRYSDVILYTSGTEDSKDVEWARRVAEELG 287
+SV +VR + ++FSGGVDS+L+A++ + DV LYT G S D ++A R A +LG
Sbjct: 218 ESVERRVRGLDEAALVFSGGVDSTLLAVLLDEHIDVRLYTVGLPGSSDPQFASRAAADLG 277
Query: 288 LKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGAD 347
++L + + I + ++ AIEE N M + +A+PLY +++ A DG +V+ SGQGAD
Sbjct: 278 MELEVLEVTEDTIREALPHVLGAIEEYNLMKIGVAMPLYLASEAASADGYRVMFSGQGAD 337
Query: 348 ELFGGYAKYL------QNPGLMLKDVEELGERNLARDDKVAMLNGVETRYPYLDLPFAIL 401
ELF GY +Y + + D+E + NL RDD V M N VE R P+LDL +
Sbjct: 338 ELFAGYHRYRRLLAEGRLEDALRHDLENIHHVNLERDDAVTMANSVELRVPFLDLQLIGV 397
Query: 402 ALNVPLELKIR---DGKRKFILREIAKSLGLPEWVAEREKKAAQYGSNAQKILEK 453
AL +P +LKIR D RK ILRE A +G+PE++A R KKAAQYGS K+L +
Sbjct: 398 ALTIPADLKIRGPEDELRKRILREAALEMGVPEYIAMRPKKAAQYGSGIDKVLRR 452
>gi|11499034 asparagine synthetase (asnB) [Archaeoglobus fulgidus]
>gi|7482912|pir||F69429 asparagine synthetase (asnB)
homolog - Archaeoglobus fulgidus
>gi|2649131|gb|AAB89808.1| (AE001004) asparagine
synthetase (asnB) [Archaeoglobus fulgidus]
Length = 371
Score = 160 bits (400), Expect = 4e-38
Identities = 112/326 (34%), Positives = 174/326 (53%), Gaps = 19/326 (5%)
Query: 138 LRGDYAVAFFFNGKFYLFRDPLGVRPLYYSSRGHFASEKKVLWGLGEDAEPVLPGEIVSI 197
+R Y+V F +L RD LG RPLYY S +S K + G + V+PGE+V +
Sbjct: 53 IRNFYSVVAFRPSHIFLSRDVLGGRPLYYGSDMSISSFKSYIDG---EVYEVMPGEVVKM 109
Query: 198 SKHG-ITRVRGFNVFSIRTQGKDYFNGLQNLLIDSVKVRASKKVGVLFSGGVDSSLIALI 256
G I R + + G++ N + + +++S+ + + FSGGVDSSL+A +
Sbjct: 110 DYTGEILERRKYAFEDVFRVGREDVNEMADRILNSLSSYKPRVGCIAFSGGVDSSLLAAL 169
Query: 257 ASRYSDVILYTSGTEDSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNP 316
D L + D+++ EW + A LG ++ I ++ + IE +
Sbjct: 170 Y----DAPLVSVTASDAEE-EWLKTAARMLGKEIEILKVGESAIREVVDTVRKTIETGDF 224
Query: 317 MNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGYAKY-----LQNPGLMLKDVEELG 371
+ L+IA+P+Y + + A+ G ++ GQGADELFGGY +Y + ++KD++ +G
Sbjct: 225 LQLSIAVPVYLTMQFAKSLGFTEIIFGQGADELFGGYKRYEGLSEHELEDALVKDLKSIG 284
Query: 372 ERNLARDDKVAMLNGVETRYPYLDLPFAILALNVPLELKIR--DGK--RKFILREIAKSL 427
E NL RD K+A N ++ PYL AL +P E K+R GK RK+ILR+IA+
Sbjct: 285 ESNLVRDCKLAYKNEIKLVTPYLCWDVIDAALKIPPEYKVRREGGKVVRKYILRKIAEKF 344
Query: 428 GLPEWVAEREKKAAQYGSNAQKILEK 453
LPE +A R+KKA QY + KIL+K
Sbjct: 345 -LPEEIAWRDKKAIQYSTGIAKILKK 369
>pir||H82255 asparagine synthetase B, glutamine-hydrolyzing VC0991 [imported] -
Vibrio cholerae (group O1 strain N16961)
>gi|9655452|gb|AAF94152.1| (AE004180) asparagine
synthetase B, glutamine-hydrolyzing [Vibrio cholerae]
Length = 554
Score = 149 bits (373), Expect = 6e-35
Identities = 142/483 (29%), Positives = 226/483 (46%), Gaps = 76/483 (15%)
Query: 1 MCLIAGGFNVS------REDAIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEGGE 54
MC I G ++ R A+++ +HRGPD G ++ + +
Sbjct: 1 MCSIFGILDIKTDAATLRPIALEMSKKLRHRGPDWSGIYASDKAI--------------- 45
Query: 55 IALIQCRLAMTGSKSYTQPFYN---EIILVHNGEIYNHESLRKYLIERGVAFETDVDSEV 111
L RLA+ G S QP Y+ ++IL NGEIYNH+ +R+ + F+TD D EV
Sbjct: 46 --LAHERLAIVGLNSGAQPLYSPDRKLILAVNGEIYNHKEIRERY-KGQYEFQTDSDCEV 102
Query: 112 ILRLLEYLVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGK--FYLFRDPLGVRPLY--YS 167
IL L D ++ L G +A + K + + RD +G+ PLY +
Sbjct: 103 ILALYRDKGADL-----------LEELNGIFAFVLYDEEKDEYLIGRDHIGIIPLYQGHD 151
Query: 168 SRGHF--ASEKKVLWGLGEDAEPVLPGEIVSISKHGITR--VRGFNVFSIRTQGKDYFNG 223
G+F ASE K L + + PG +S +TR VR +N F
Sbjct: 152 EHGNFYVASEMKALVPVCKTLSEFPPGSYLSSKDQAVTRYYVRDWNSFDEVKDNVSSKEE 211
Query: 224 LQNLLIDSVKVRASKKV--GVLFSGGVDSSLIALIASRYSDVIL---------------Y 266
L L +VK + V GVL SGG+DSS+ + IA R++ + + +
Sbjct: 212 LTQALEAAVKRQLMTDVPYGVLLSGGLDSSITSAIAKRFAAMRIEDDEKSAAWWPQLHSF 271
Query: 267 TSGTEDSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLY 326
G E + D++ AR VAE++G E ++ ++ + I +++ IE + + + P++
Sbjct: 272 AIGLEGAPDLKAAREVAEKIGTVHHEMTYTIQEGLDAIRDVIYHIETYDVTTIRASTPMF 331
Query: 327 FSTKRAREDGVKVLLSGQGADELFGGYAKYLQNPGLMLKDVEELGERNL--------ARD 378
++ + G+K++LSG+GADE+FGGY + + P K+ E R L AR
Sbjct: 332 LMGRKIKAMGIKMVLSGEGADEIFGGYLYFHKAPN--AKEFHEETVRKLLALNLFDCARA 389
Query: 379 DKVAMLNGVETRYPYLDLPFAILALNV-PLELKIRDGK-RKFILREIAKSLGLPEWVAER 436
+K GVE R P+LD F +A+ + P + +GK K ILRE + LPE +A R
Sbjct: 390 NKSLAAWGVEGRVPFLDKEFIDVAMRLNPADKMCGNGKMEKHILRECFEHY-LPESIAWR 448
Query: 437 EKK 439
+K+
Sbjct: 449 QKE 451
>dbj|BAA89376.1| (AB025342) ORF2 [Moritella marina]
Length = 555
Score = 148 bits (371), Expect = 1e-34
Identities = 143/482 (29%), Positives = 227/482 (46%), Gaps = 74/482 (15%)
Query: 1 MCLIAGGFNVS------REDAIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEGGE 54
MC I G ++ RE A+++ L +HRGPD G ++ SDN
Sbjct: 1 MCSIFGILDIKSDIKPLREQALELSKLLRHRGPDWSGIYT------SDN----------- 43
Query: 55 IALIQCRLAMTGSKSYTQPFYNEI---ILVHNGEIYNHESLRKYLIERGVAFETDVDSEV 111
L+ RLA+ + QP YNE +L NGEIYNH+ L+K L F+T+ D EV
Sbjct: 44 AILVHERLAIVDVNNGAQPLYNEEKTHVLAVNGEIYNHKDLKKTL-NVDFEFQTESDCEV 102
Query: 112 ILRLLEYLVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGK--FYLFRDPLGVRPLY--YS 167
IL L + E + L G +A A + + + + RD +G+ PLY Y
Sbjct: 103 ILALYK-----------EKGTQFLDDLNGIFAFALYDETEDAYLIGRDHIGIIPLYTGYD 151
Query: 168 SRGHF--ASEKKVLWGLGEDAEPVLPGEIVSISKHGITRV---RGFNVFSIRTQGKDYFN 222
G+F ASE K L + E G + SK G+ R + F Q +
Sbjct: 152 EHGNFYVASEMKALVPICTQIEEFPAGHYLW-SKDGVVTPYYQRDWKDFDNVAQNGGDKS 210
Query: 223 GLQNLLIDSVKVRASKKV--GVLFSGGVDSSLIALIASRYS---------------DVIL 265
++ L D+VK + V GVL SGG+DSS+I+ I +Y+ +
Sbjct: 211 VVKQGLEDAVKRQLMCDVPYGVLLSGGLDSSVISAITQQYAKRRIEDGGKTEAWWPQLHS 270
Query: 266 YTSGTEDSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPL 325
++ G S D+ A++VA+ LG F+ ++ + + +++ IE + + + P+
Sbjct: 271 FSVGLNGSPDLAAAQKVADHLGTIHHSIEFTVQEGIDALRDVIYHIETYDVTTIRASTPM 330
Query: 326 YFSTKRAREDGVKVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDD 379
Y ++ + G+K++LSG+GADELFGGY + + P ++ V +L + R +
Sbjct: 331 YLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAKEFHEETVRKVNKLHMFDCLRAN 390
Query: 380 KVAMLNGVETRYPYLDLPFAILALNVPLELKIRDGKR--KFILREIAKSLGLPEWVAERE 437
K G+E R P+LD F ++ + ELK+ G R K I+RE + L LPE + R+
Sbjct: 391 KSMAAWGIEARVPFLDKEFVDASMRLNPELKMITGDRIEKNIIREAFEDL-LPEEIVWRQ 449
Query: 438 KK 439
K+
Sbjct: 450 KE 451
>sp|P22106|ASNB_ECOLI ASPARAGINE SYNTHETASE B [GLUTAMINE-HYDROLYZING]
>gi|68659|pir||AJECN asparagine synthase
(glutamine-hydrolyzing) (EC 6.3.5.4) - Escherichia coli
>gi|145393|gb|AAA23498.1| (J05554) asparagine synthetase
B [Escherichia coli] >gi|1651277|dbj|BAA35317.1|
(D90706) Asparagine synthase (glutamine-hydrolyzing) (EC
6.3.5.4) [Escherichia coli] >gi|1786889|gb|AAC73768.1|
(AE000171) asparagine synthetase B [Escherichia coli
K12]
Length = 554
Score = 145 bits (362), Expect = 1e-33
Identities = 147/485 (30%), Positives = 230/485 (47%), Gaps = 80/485 (16%)
Query: 1 MCLIAGGFNVS------REDAIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEGGE 54
MC I G F++ R+ A+++ L +HRGPD G ++ SDN
Sbjct: 1 MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYA------SDN----------- 43
Query: 55 IALIQCRLAMTGSKSYTQPFYNEI---ILVHNGEIYNHESLRKYLIERGVAFETDVDSEV 111
L RL++ + QP YN+ +L NGEIYNH++LR +R F+T D EV
Sbjct: 44 AILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDR-YQFQTGSDCEV 102
Query: 112 ILRLLEYLVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGK--FYLFRDPLGVRPLY--YS 167
IL L + E + L+G +A A + + K + + RD LG+ PLY Y
Sbjct: 103 ILALYQ-----------EKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYD 151
Query: 168 SRG--HFASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVR----GFNVFSIRTQGKDYF 221
G + ASE K L + + G + S+ G R F+ +++ D
Sbjct: 152 EHGQLYVASEMKALVPVCRTIKEFPAGSYLW-SQDGEIRSYYHRDWFDYDAVKDNVTDK- 209
Query: 222 NGLQNLLIDSVKVRASKKV--GVLFSGGVDSSLIALIASRYS---------------DVI 264
N L+ L DSVK V GVL SGG+DSS+I+ I +Y+ +
Sbjct: 210 NELRQALEDSVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLH 269
Query: 265 LYTSGTEDSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIP 324
+ G S D++ A+ VA LG E F+ ++ + I +++ IE + + + P
Sbjct: 270 SFAVGLPGSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTP 329
Query: 325 LYFSTKRAREDGVKVLLSGQGADELFGGYAKYLQNPGLMLKDVEELGERNL--------A 376
+Y +++ + G+K++LSG+G+DE+FGGY + + P K++ E R L A
Sbjct: 330 MYLMSRKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPN--AKELHEETVRKLLALHMYDCA 387
Query: 377 RDDKVAMLNGVETRYPYLDLPFAILALNV-PLELKIRDGK-RKFILREIAKSLGLPEWVA 434
R +K GVE R P+LD F +A+ + P + +GK K ILRE ++ LP VA
Sbjct: 388 RANKAMSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAY-LPASVA 446
Query: 435 EREKK 439
R+K+
Sbjct: 447 WRQKE 451
>pir||T16625 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) M02D8.4
[similarity] - Caenorhabditis elegans
>gi|1086745|gb|AAA82381.1| (U41034) coded for by C.
elegans cDNA yk61f9.5; coded for by C. elegans cDNA
yk80c1.5; coded for by C. elegans cDNA yk4g5.5; coded
for by C. elegans cDNA yk127h1.5; coded for by C.
elegans cDNA yk37d7.5; coded for by C. elegans cDNA
yk55e11.5; coded fo>
Length = 567
Score = 143 bits (358), Expect = 3e-33
Identities = 136/438 (31%), Positives = 206/438 (46%), Gaps = 56/438 (12%)
Query: 13 EDAIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLAMTGSKSYTQ 72
++A K+ HRGPD G + + + G+I L+ RLA+ Q
Sbjct: 21 DEARKLSRRQHHRGPDFSGCYQDTKT--------------GDI-LVHERLAIM-DLGVVQ 64
Query: 73 PFYNEII---LVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEE 129
P ++HNGEIYNH+ LR +++ +T DSEVI+ L E K +
Sbjct: 65 PLQGTAPNRQVIHNGEIYNHQELRDTELKK-YNLKTHCDSEVIIFLYE------KYRDGH 117
Query: 130 AIKLSMKMLRGDYAVAFFFNGKFYLFRDPLGVRPLYYS----SRGHFASEKKVLW-GLGE 184
L L G +A +G F RDPLGV+ +YY R F++E K L G+
Sbjct: 118 ICNL----LDGVFAFVLCCDGDFLAARDPLGVKQMYYGIDDDGRYFFSNEMKCLEDSCGD 173
Query: 185 DAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDY-FNGLQNLLIDSVKVR--ASKKVG 241
+ P K G R F R LQ +I SV R + +G
Sbjct: 174 NKIESFPPGHYYTPKTGFVRYFNPEWFDFRKAIHPLDLKLLQKTMIASVHKRLMSDAPIG 233
Query: 242 VLFSGGVDSSLIALIASRYSD-----VILYTSGTE-DSKDVEWARRVAEELGLKLRESLF 295
VL SGG+DSSL++ IASR V ++ G + +S DV AR+VA+ +G E F
Sbjct: 234 VLLSGGLDSSLVSSIASREMKRRGMAVHSFSIGVDHNSPDVVAARKVAKFIGTTHHEFYF 293
Query: 296 SREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGYAK 355
S E+ + ++++ +E + ++ + P+YF ++ R+ G+KV+LSG+GADE+FGGY
Sbjct: 294 SIEEGIKNLRKLIWHLESYDVTSIRASTPMYFLSEEIRKLGIKVVLSGEGADEIFGGYLY 353
Query: 356 YLQNPG------LMLKDVEELGERNLARDDKVAMLNGVETRYPYLDLPFAILALNVPLEL 409
+ P + V L + R DK +M + VE R P+LD F A+++
Sbjct: 354 FHNAPSDEDFQKETIDRVLHLYTSDCLRADKSSMAHSVEVRVPFLDKAFVEAAVSLDPAF 413
Query: 410 ----KIRDGK--RKFILR 421
K+ DG+ KF+LR
Sbjct: 414 KRPQKLEDGRNCEKFVLR 431
>gb|AAF02776.1|AF190729_1 (AF190729) asparagine synthetase [Helianthus annuus]
Length = 558
Score = 142 bits (354), Expect = 1e-32
Identities = 139/475 (29%), Positives = 217/475 (45%), Gaps = 69/475 (14%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ +I+ L +HRGPD G SE + L
Sbjct: 7 VFGCVDCSQAKRARILELSSRLRHRGPDWSGLHSEE-----------------DCYLAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP YNE II+ NGEIYNH+ R+ L + F T D EVI L E
Sbjct: 50 RLAIVDPASGDQPLYNEDKSIIVTVNGEIYNHKEFREQL--KSHKFNTGSDCEVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G ++ N + RD +G+ PLY
Sbjct: 108 -----------EHGEDFVHMLDGMFSFVLLDTRNKSYIAARDAIGITPLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFN-GLQNLLID 230
FASE K L E LPG I S G+ R +S Y L+N
Sbjct: 157 FASEMKALSDDCEQFMSFLPGHIYSSKNGGLRRWYNPPWWSELVPSTPYDPLVLRNAFEK 216
Query: 231 SVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKDVE 277
+V R V GVL SGG+DSSL+A +ASR+ S + + G + S D+
Sbjct: 217 AVIKRLMTDVPFGVLLSGGLDSSLVAAVASRHLVDSEAYCQWGSQLHTFCIGLKGSPDLV 276
Query: 278 WARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGV 337
AR VA+ LG + E F+ ++ + +E +++ IE + + + P++ +++ + GV
Sbjct: 277 AAREVADYLGTRHHEFYFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGV 336
Query: 338 KVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRY 391
K++LSG+G+DE+FGGY + + P + ++ L + R +K G+E R
Sbjct: 337 KMVLSGEGSDEIFGGYLYFHKAPNKEEFHEETCRKIKALHLYDCLRANKSTSAWGLEARV 396
Query: 392 PYLDLPFAILALNVPLELKI--RDGKR--KFILR---EIAKSLGLPEWVAEREKK 439
P+LD F +A+++ + K+ RD R K++LR + + LP+ + R+K+
Sbjct: 397 PFLDKEFINVAMSIDPKWKMIDRDNGRIEKWVLRNAFDDDEKPYLPKHILYRQKE 451
>pdb|1CT9|A Chain A, Crystal Structure Of Asparagine Synthetase B From
Escherichia Coli >gi|6730196|pdb|1CT9|B Chain B, Crystal
Structure Of Asparagine Synthetase B From Escherichia
Coli >gi|6730197|pdb|1CT9|C Chain C, Crystal Structure
Of Asparagine Synthetase B From Escherichia Coli
>gi|6730198|pdb|1CT9|D Chain D, Crystal Structure Of
Asparagine Synthetase B From Escherichia Coli
Length = 553
Score = 141 bits (352), Expect = 2e-32
Identities = 142/468 (30%), Positives = 223/468 (47%), Gaps = 74/468 (15%)
Query: 12 REDAIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLAMTGSKSYT 71
R+ A+++ L +HRGPD G ++ SDN L RL++ +
Sbjct: 17 RKKALELSRLMRHRGPDWSGIYA------SDN-----------AILAHERLSIVDVNAGA 59
Query: 72 QPFYNEI---ILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVE 128
QP YN+ +L NGEIYNH++LR +R F+T D EVIL L +
Sbjct: 60 QPLYNQQKTHVLAVNGEIYNHQALRAEYGDR-YQFQTGSDCEVILALYQ----------- 107
Query: 129 EAIKLSMKMLRGDYAVAFFFNGK--FYLFRDPLGVRPLY--YSSRG--HFASEKKVLWGL 182
E + L+G +A A + + K + + RD LG+ PLY Y G + ASE K L +
Sbjct: 108 EKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVASEMKALVPV 167
Query: 183 GEDAEPVLPGEIVSISKHGITRVR----GFNVFSIRTQGKDYFNGLQNLLIDSVKVRASK 238
+ G + S+ G R F+ +++ D N L+ L DSVK
Sbjct: 168 CRTIKEFPAGSYLW-SQDGEIRSYYHRDWFDYDAVKDNVTDK-NELRQALEDSVKSHLMS 225
Query: 239 KV--GVLFSGGVDSSLIALIASRYS---------------DVILYTSGTEDSKDVEWARR 281
V GVL SGG+DSS+I+ I +Y+ + + G S D++ A+
Sbjct: 226 DVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLPGSPDLKAAQE 285
Query: 282 VAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLL 341
VA LG E F+ ++ + I +++ IE + + + P+Y +++ + G+K++L
Sbjct: 286 VANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMSRKIKAMGIKMVL 345
Query: 342 SGQGADELFGGYAKYLQNPGLMLKDVEELGERNL--------ARDDKVAMLNGVETRYPY 393
SG+G+DE+FGGY + + P K++ E R L AR +K GVE R P+
Sbjct: 346 SGEGSDEVFGGYLYFHKAPN--AKELHEETVRKLLALHMYDCARANKAMSAWGVEARVPF 403
Query: 394 LDLPFAILALNV-PLELKIRDGK-RKFILREIAKSLGLPEWVAEREKK 439
LD F +A+ + P + +GK K ILRE ++ LP VA R+K+
Sbjct: 404 LDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAY-LPASVAWRQKE 450
>sp|P49092|ASN1_LOTJA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 1
(GLUTAMINE-DEPENDENT ASPARAGINE SYNTHETASE 1)
>gi|2147131|pir||S57931 asparagine synthase
(glutamine-hydrolysing) (EC 6.3.5.4) - Lotus japonicus
>gi|2147133|pir||S69182 asparagine synthase
(glutamine-hydrolyzing) (EC 6.3.5.4) 1 - Lotus japonicus
>gi|897771|emb|CAA61589.1| (X89409) asparagine synthase
(glutamine-hydrolysing) [Lotus japonicus]
Length = 586
Score = 141 bits (352), Expect = 2e-32
Identities = 139/475 (29%), Positives = 216/475 (45%), Gaps = 69/475 (14%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + ++ ++++ L KHRGPD WS + G+ L
Sbjct: 7 VLGCSDFTQAKRVRVLELSRRLKHRGPD----WSGLH-------------QHGDCYLAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE II+ NGEIYNHE LRK L F T D +VI L E
Sbjct: 50 RLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEELRKQLPNH--QFRTGSDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + M ML G ++ + F + RD +GV LY
Sbjct: 108 -----------EHGENFMDMLDGIFSFVLLDTRDNTFIVARDAIGVTSLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFN-GLQNLLID 230
+SE K L E E PG + S + R FS Y +++
Sbjct: 157 ISSEMKGLNDDCEHFEVFPPGHLYSSRERAFRRWYNPTWFSESIPSAPYDPLAVRHAFEK 216
Query: 231 SVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKDVE 277
+V R V GVL SGG+DSSL+A I SRY S + + G E S D++
Sbjct: 217 AVIKRLMTDVPFGVLLSGGLDSSLVASITSRYLATTKAAEQWGSKLHSFCVGLEGSPDLK 276
Query: 278 WARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGV 337
A+ VA+ LG E F+ +D + IE +++ +E + + + P++ +++ + GV
Sbjct: 277 AAKEVADYLGTVHHEFTFTVQDGIDAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGV 336
Query: 338 KVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRY 391
K ++SG+G+DE+FGGY + + P + ++ L + + R +K G+E R
Sbjct: 337 KWVISGEGSDEIFGGYLYFHKAPNKEEFHTETCRKIKALHQYDCLRANKSTFAWGLEARV 396
Query: 392 PYLDLPFAILALNVPLELKI--RDGKR--KFILR---EIAKSLGLPEWVAEREKK 439
P+LD F +A+N+ E K+ RD R K+ILR + + LP+ + R+K+
Sbjct: 397 PFLDKEFINVAMNIDPEYKMIKRDEGRIEKYILRRAFDDEEKPYLPKHILYRQKE 451
>emb|CAA08913.1| (AJ009952) asparagine synthetase type II [Phaseolus vulgaris]
Length = 584
Score = 139 bits (348), Expect = 5e-32
Identities = 138/475 (29%), Positives = 211/475 (44%), Gaps = 69/475 (14%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + SR ++++ L KHRGPD WS + G+ L
Sbjct: 7 VLGCSDHSRAKRVRVLELSRRLKHRGPD----WSGLH-------------QHGDCFLAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE +I+ NGEIYNHE LRK L G F T D +VI L E
Sbjct: 50 RLAIVDPASGDQPLFNEDKSVIVTVNGEIYNHEELRKQL--PGHKFRTGSDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G ++ + F + RD +GV LY
Sbjct: 108 -----------EHGEGFVDMLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFNGLQNLLIDS 231
+SE K L E E PG + S G R FS Y + +
Sbjct: 157 ISSEMKGLNDDCEHFECFPPGHLYSSKDRGFRRWYNPPWFSEAIPSAPYDPLVLRRAFEQ 216
Query: 232 VKVR---ASKKVGVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKDVE 277
++ GVL SGG+DSSL+A I SRY S + + G E S D++
Sbjct: 217 AVIKRLMTGVPFGVLLSGGLDSSLVASITSRYLANTKAAEQWGSKLHSFCVGLEGSPDLK 276
Query: 278 WARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGV 337
A+ VA+ LG E F+ +D + IE +++ +E + + + P++ +++ + GV
Sbjct: 277 AAKEVADYLGTVHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGV 336
Query: 338 KVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRY 391
K ++SG+G+DE+FGGY + + P + ++ L + + R +K G+E R
Sbjct: 337 KWVISGEGSDEIFGGYLYFHKAPNKEEFHRETCRKIKALHQYDCLRANKSTFAWGLEARV 396
Query: 392 PYLDLPFAILALNVPLELKI--RDGKR--KFILREIAKSLG---LPEWVAEREKK 439
P+LD F A+N+ E K+ RD R K+ILR LP+ + R+K+
Sbjct: 397 PFLDKEFINAAMNIDPEHKMIKRDEGRIEKWILRRAFDDEDQPYLPKHILYRQKE 451
>pir||T08846 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) 2 -
soybean >gi|1778370|gb|AAC49613.1| (U77678) asparagine
synthetase 2 [Glycine max]
Length = 581
Score = 139 bits (347), Expect = 6e-32
Identities = 141/475 (29%), Positives = 216/475 (44%), Gaps = 69/475 (14%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + SR ++++ L KHRGPD WS + G+ L
Sbjct: 7 VLGCSDDSRAKRVRVLELSRRLKHRGPD----WSGLH-------------QHGDCFLAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE +I+ NGEIYNHE LRK L F T D +VI L E
Sbjct: 50 RLAIVDPASGDQPLFNEDKSVIVTVNGEIYNHEELRKQLPNHN--FRTGSDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G ++ + F + RD +GV LY
Sbjct: 108 -----------EHGEDFVDMLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFN-GLQNLLID 230
+SE K L E E PG + S + G R FS Y L++
Sbjct: 157 ISSEMKGLNDDCEHFECFPPGHLYSSKERGFRRWYNPPWFSEAIPSAPYDPLVLRHAFEQ 216
Query: 231 SVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKDVE 277
+V R V GVL SGG+DSSL+A I SRY S + + G E S D++
Sbjct: 217 AVIKRLMTDVPFGVLLSGGLDSSLVASITSRYLANTKAAEQWGSKLHSFCVGLEGSPDLK 276
Query: 278 WARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGV 337
A+ VA+ LG E F+ +D + IE +++ IE + + + P++ +++ + GV
Sbjct: 277 AAKEVADYLGTVHHEFTFTVQDGIDSIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGV 336
Query: 338 KVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRY 391
K ++SG+G+DE+FGGY + + P + ++ L + + R +K G+E R
Sbjct: 337 KWVISGEGSDEIFGGYLYFHKAPNKEEFHRETCRKIKALHQYDCLRANKSTFAWGLEARV 396
Query: 392 PYLDLPFAILALNVPLELKI--RDGKR--KFILR---EIAKSLGLPEWVAEREKK 439
P+LD F A+++ E K+ RD R K+ILR + + LP+ + R+K+
Sbjct: 397 PFLDKAFINAAMSIDPEWKMIKRDEGRIEKWILRRAFDDEEHPYLPKHILYRQKE 451
>gb|AAC25799.1| (AF022973) Contains similarity to Pfam domain: PF00310 (GATase_2),
Score=44.2, E-value=6.3e-15, N=3 [Caenorhabditis
elegans]
Length = 551
Score = 138 bits (345), Expect = 1e-31
Identities = 122/405 (30%), Positives = 199/405 (49%), Gaps = 41/405 (10%)
Query: 62 LAMTGSKSYTQPFYNE-IILVHNGEIYNHESLR---KYLIERGVAFETDVDSEVILRLLE 117
+ M G QP + +V NGEIYNH+SL+ + ++ G + D I+
Sbjct: 49 IVMPGDTPSAQPIVGGGLSVVCNGEIYNHQSLKIDCPFTLKNGGS-----DCAAIITSF- 102
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLFRDPLGVRPLYYSSRGH----FA 173
+K + +K + L G +A + Y+ RDP+GVRPL+Y +
Sbjct: 103 -------IKHNKDLKEACASLDGVFAFIMADDKNVYIGRDPIGVRPLFYGYNSNGSLLIG 155
Query: 174 SEKKVLWGLGEDAEPVLPGEIVSISKHGITR-VRGFNVFSIRTQGKDYFNG------LQN 226
SE K + L E E PG +IS TR V+ +S+ T + + +++
Sbjct: 156 SEVKCIEELCERVEYFPPGCCATISLFAPTRPVQPQQYYSVPTIADRFLSIDCTKTLVRD 215
Query: 227 LLIDSVKVR--ASKKVGVLFSGGVDSSLIALIASRY--SDVILYTSGTEDSKDVEWARRV 282
+L+ SV+ R ++ G + SGG+DSSLIA IA+R+ I ++ G EDS D+E A++V
Sbjct: 216 VLVKSVEKRLMGNRNFGFMLSGGLDSSLIASIATRFLKQKPIAFSVGFEDSPDLENAKKV 275
Query: 283 AEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKR-AREDGVKVLL 341
A+ L + + + + + I ++FA+E +P+ + I Y + ++ VKVLL
Sbjct: 276 ADYLKIPHEVLVITPQQCIDIIPEVVFALETFDPLIIRCGIAHYLLCQHISKSSDVKVLL 335
Query: 342 SGQGADELFGGYAKYLQNPGLM------LKDVEELGERNLARDDKVAMLNGVETRYPYLD 395
SG+GADELFG YA + P + L+ + L + ++ R D+ +G+E R P+LD
Sbjct: 336 SGEGADELFGSYAYMQRAPNALHLHKEILRRMHHLHQYDVLRCDRSTSCHGLEIRVPFLD 395
Query: 396 LPFAILALNVPLELKIRDGK-RKFILREIAKSLGLPEWVAEREKK 439
F L +P K+ K K +LR + LP+ V R K+
Sbjct: 396 KRFIDLVSRLPPSYKLMPMKLEKHVLRSAFEG-WLPDEVLWRSKE 439
>sp|P19252|ASN2_PEA ASPARAGINE SYNTHETASE, ROOT [GLUTAMINE-HYDROLYZING]
(GLUTAMINE-DEPENDENT ASPARAGINE SYNTHETASE)
>gi|68660|pir||AJPMN2 asparagine synthase
(glutamine-hydrolyzing) (EC 6.3.5.4) 2 - garden pea
>gi|20652|emb|CAA36430.1| (X52180) asparagine synthase
(glutamine-hydrolysing) [Pisum sativum]
Length = 583
Score = 138 bits (344), Expect = 1e-31
Identities = 138/475 (29%), Positives = 217/475 (45%), Gaps = 69/475 (14%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + SR ++++ L KHRGP+ WS + G+ L Q
Sbjct: 7 VLGCSDPSRAKRVRVLELSRRLKHRGPE----WSGLH-------------QHGDCYLAQQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE I+ NGEIYNHE LRK L F T D +VI L E
Sbjct: 50 RLAIVDPASGDQPLFNEDNPSIVTVNGEIYNHEDLRKQLSNH--TFRTGSDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
D + ML G ++ + + + RD +GV LY
Sbjct: 108 EYGED-----------FVDMLDGIFSFVPLDTRDNSYIVARDAIGVTSLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFN-GLQNLLID 230
+SE K L E E PG + S G R + +S Y L++
Sbjct: 157 ISSEMKGLNDDCEHFECFPPGHLYSSKDSGFRRWYNPSWYSEAIPSAPYDPLALRHAFEK 216
Query: 231 SVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKDVE 277
+V R V GVL SGG+DSSL+A I SRY S + + G E S D++
Sbjct: 217 AVVKRLMTDVPFGVLLSGGLDSSLVASITSRYLATTKAAEQWGSKLHSFCVGLEGSPDLK 276
Query: 278 WARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGV 337
+ VA+ LG E F+ +D + IE +++ +E + ++ + P++ +++ + GV
Sbjct: 277 AGKEVADYLGTVHHEFTFTVQDGIDAIEDVIYHVETYDVTSIRASTPMFLMSRKIKSLGV 336
Query: 338 KVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRY 391
K ++SG+G+DE+FGGY + + P + ++ L + + R +K G+E R
Sbjct: 337 KWVISGEGSDEIFGGYLYFHKAPNKEEFHEETCRKIKALHQYDCQRANKSTYAWGLEARV 396
Query: 392 PYLDLPFAILALNVPLELKI--RDGKR--KFILREI---AKSLGLPEWVAEREKK 439
P+LD F +A+N+ E K+ RD R K+ILR+ ++ LP+ + R+K+
Sbjct: 397 PFLDKAFINVAMNIDPENKMIKRDEGRIEKYILRKAFDDEENPYLPKHILYRQKE 451
>gb|AAF02775.1|AF190728_1 (AF190728) asparagine synthetase [Helianthus annuus]
Length = 591
Score = 138 bits (344), Expect = 1e-31
Identities = 135/475 (28%), Positives = 214/475 (44%), Gaps = 67/475 (14%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++++ L KHRGPD G + DN+ L
Sbjct: 7 VLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQH-----GDNY------------LSHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE I++ NGEIYNHE LRK L G F T D +VI L E
Sbjct: 50 RLAIIDPASGDQPLFNEDQTIVVTVNGEIYNHEELRKRLTAAGHKFRTGSDCDVIAHLYE 109
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G ++ N + RD +G+ LY
Sbjct: 110 -----------EHGEDFVDMLDGMFSFVLLDTRNNTYIAARDAIGITSLYIGWGLDGSVW 158
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFNGLQNLLIDS 231
+SE K L E E PG + S G R F+ Y + ++
Sbjct: 159 ISSELKGLNDDCEHFEVFPPGHLYSSKTGGFKRWYNPLWFAETIPSTPYDPLVLRCAFEN 218
Query: 232 VKVR---ASKKVGVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKDVE 277
++ GVL SGG+DSSL+A I +RY + + + G E S D++
Sbjct: 219 AVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTKAAKQWGAQLHSFCVGLEGSPDLK 278
Query: 278 WARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGV 337
AR VA+ LG E F+ +D + IE +++ IE + + + P++ +++ + GV
Sbjct: 279 AAREVADYLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGV 338
Query: 338 KVLLSGQGADELFGGYA---KYLQNPGL---MLKDVEELGERNLARDDKVAMLNGVETRY 391
K+++SG+G+DE+FGGY K L + ++ L + + R +K G+E R
Sbjct: 339 KMVISGEGSDEIFGGYLYFHKALNREEFHQETCRKIKALHQYDCLRANKSTSAWGLEARV 398
Query: 392 PYLDLPFAILALNVPLELKI--RDGKR--KFILR---EIAKSLGLPEWVAEREKK 439
P+LD F +A+++ E K+ D KR K++LR E + LP+ + R+K+
Sbjct: 399 PFLDKEFINVAMSIDPEAKMINMDQKRIEKWVLRRAFEDEEHPYLPKHILYRQKE 453
>gb|AAC72836.1| (AF095452) asparagine synthetase [Arabidopsis thaliana]
Length = 578
Score = 138 bits (343), Expect = 2e-31
Identities = 139/480 (28%), Positives = 219/480 (44%), Gaps = 79/480 (16%)
Query: 4 IAGGFNVSREDAIKIINLGK---HRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ +II L + HRGPD WS + + L
Sbjct: 7 VLGCVDNSQAKRSRIIELSRRLRHRGPD----WSGLHCYE-------------DCYLAHE 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP YNE I + NGEIYNH+ LR+ L + F T D EVI L E
Sbjct: 50 RLAIVDPTSGDQPLYNEDKTIAVTVNGEIYNHKDLRENL--KSHQFRTGSDCEVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G +A + F RD +G+ PLY
Sbjct: 108 -----------EHGEEFVDMLDGMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVL---PGEIVSISKHGITRVRGFNVFSIRTQGKDYF-----NG 223
FASE K L +D E + PG I S + G+ R FS Y N
Sbjct: 157 FASEMKAL---SDDCEQFMCFPPGHIYSSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNT 213
Query: 224 LQNLLIDSVKVRASKKVGVLFSGGVDSSLIALIASRY-----------SDVILYTSGTED 272
+ +I ++ +GVL SGG+DSSL+A +A R+ S + + G +
Sbjct: 214 FEKAVIK--RLMTDVPLGVLLSGGLDSSLVASVALRHLEKSEAACQWGSKLHTFCIGLKG 271
Query: 273 SKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRA 332
S D++ R VA+ LG + E F+ +D + IE +++ +E + + + P++ +++
Sbjct: 272 SPDLKAGREVADYLGTRHHELHFTVQDGIDAIEEVIYHVETYDVTTIRASTPMFLMSRKI 331
Query: 333 REDGVKVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNG 386
+ GVK++LSG+G+DE+FGGY + + P + ++ L + + R +K G
Sbjct: 332 KSLGVKMVLSGEGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHQYDCLRANKSTSAWG 391
Query: 387 VETRYPYLDLPFAILALNVPLELK-IRDGK---RKFILR---EIAKSLGLPEWVAEREKK 439
VE R P+LD F +A+++ E K IR K++LR + K+ LP+ + R+K+
Sbjct: 392 VEARVPFLDKEFINVAMSIDPEWKMIRPDLGRIEKWVLRNAFDDEKNPYLPKHILYRQKE 451
>sp|O24338|ASNS_SANAU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|2245622|gb|AAB71532.1|
(AF005724) asparagine synthetase [Sandersonia
aurantiaca]
Length = 525
Score = 138 bits (343), Expect = 2e-31
Identities = 133/443 (30%), Positives = 202/443 (45%), Gaps = 68/443 (15%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++++ L KHRGPD WS G+ L
Sbjct: 7 VLGCSDDSQAKRVRVLELSRRLKHRGPD----WSGLD-------------HHGDCYLAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP YNE II+ NGEIYNHE LRK L G F T D EVI L E
Sbjct: 50 RLAIIDPASGDQPLYNEDKTIIVTVNGEIYNHEELRKGL--PGHTFRTGSDCEVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G ++ N F RD +GV PLY
Sbjct: 108 -----------EHGESFIHMLDGIFSFVLLDSRNNSFVAARDAIGVTPLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVL---PGEIVSISKHGITRVRGFNVFSIRTQGKDYFN-GLQNL 227
+SE K GL +D E PG + S + R FS + L+
Sbjct: 157 ISSEMK---GLNDDCEHFKFFPPGHLYSSKEGSFKRWYNPPWFSEVIPSVPFDPLALRKA 213
Query: 228 LIDSVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSK 274
D+V R V GVL SGG+DSSL+A + +RY + + + G E S
Sbjct: 214 FEDAVIKRLMTDVPFGVLLSGGLDSSLVASVTARYLEGTKAAELWGTQLHSFCVGLEGSP 273
Query: 275 DVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRARE 334
D++ A+ VA LG E F+ +D + IE +++ +E + + + P++ +++ +
Sbjct: 274 DLKAAKEVANFLGTIHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKS 333
Query: 335 DGVKVLLSGQGADELFGGYAKYLQNPG---LMLK---DVEELGERNLARDDKVAMLNGVE 388
GVK+++SG+G+DE+FGGY + + P L L+ ++ L + + R +K G+E
Sbjct: 334 LGVKMVISGEGSDEIFGGYLYFHKAPNKEELHLETCHKIKALHQYDCLRANKATSAWGLE 393
Query: 389 TRYPYLDLPFAILALNVPLELKI 411
R P+LD F +A+++ E K+
Sbjct: 394 ARVPFLDKEFVNVAMSIDPEWKM 416
>pir||T50812 asparagine synthetase ASN3 - Arabidopsis thaliana
>gi|8953405|emb|CAB96680.1| (AL360334) asparagine
synthetase ASN3 [Arabidopsis thaliana]
Length = 578
Score = 137 bits (342), Expect = 2e-31
Identities = 141/478 (29%), Positives = 220/478 (45%), Gaps = 75/478 (15%)
Query: 4 IAGGFNVSREDAIKIINLGK---HRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ +II L + HRGPD WS + + L
Sbjct: 7 VLGCVDNSQAKRSRIIELSRRLRHRGPD----WSGLHCYE-------------DCYLAHE 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP YNE I + NGEIYNH++LR+ L + F T D EVI L E
Sbjct: 50 RLAIVDPTSGDQPLYNEDKTIAVTVNGEIYNHKALRENL--KSHQFRTGSDCEVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G +A + F RD +G+ PLY
Sbjct: 108 -----------EHGEEFVDMLDGMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVL---PGEIVSISKHGITRVRGFNVFSIRTQGKDYFN-GLQNL 227
FASE K L +D E + PG I S + G+ R FS Y ++N
Sbjct: 157 FASEMKAL---SDDCEQFMCFPPGHIYSSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNT 213
Query: 228 LIDSVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSK 274
+V R V GVL SGG+DSSL+A +A R+ S + + G + S
Sbjct: 214 FEKAVIKRLMTDVPFGVLLSGGLDSSLVASVALRHLEKSEAACQWGSKLHTFCIGLKGSP 273
Query: 275 DVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRARE 334
D++ R VA+ LG + E F+ +D + IE +++ +E + + + P++ +++ +
Sbjct: 274 DLKAGREVADYLGTRHHELHFTVQDGIDAIEEVIYHVETYDVTTIRASTPMFLMSRKIKS 333
Query: 335 DGVKVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVE 388
GVK++LSG+G+DE+FGGY + + P + ++ L + + R +K GVE
Sbjct: 334 LGVKMVLSGEGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHQYDCLRANKSTSAWGVE 393
Query: 389 TRYPYLDLPFAILALNVPLELK-IRDGK---RKFILR---EIAKSLGLPEWVAEREKK 439
R P+LD F +A+++ E K IR K++LR + K+ LP+ + R+K+
Sbjct: 394 ARVPFLDKEFINVAMSIDPEWKMIRPDLGRIEKWVLRNAFDDEKNPYLPKHILYRQKE 451
>sp|P54420|ASNB_BACSU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 1
>gi|7474447|pir||H69590 asparagine synthetase asnB -
Bacillus subtilis >gi|2293165|gb|AAC00243.1| (AF008220)
asparagine synthase [Bacillus subtilis]
>gi|2635538|emb|CAB15032.1| (Z99119) asparagine
synthetase [Bacillus subtilis]
Length = 632
Score = 135 bits (337), Expect = 9e-31
Identities = 131/419 (31%), Positives = 196/419 (46%), Gaps = 81/419 (19%)
Query: 1 MCLIAGGFN-------VSREDAIKIIN-LGKHRGPDSFGFWSERQMLKSDNFSDVSEIEG 52
MC G FN +E+ IK +N + HRGPDS G++ + + + ++E
Sbjct: 1 MCGFVGVFNKHPLAQTADQEELIKQMNQMIVHRGPDSDGYFHDEHVGFGFRRLSIIDVEN 60
Query: 53 GEIALIQCRLAMTGSKSYTQPFYNEIILVHNGEIYNHESLRKYLIERGVAFETDVDSEVI 112
G L SY Y ++ NGEIYN+ LR+ L +G F TD D+EV+
Sbjct: 61 GGQPL-----------SYEDETY---WIIFNGEIYNYIELREELEAKGYTFNTDSDTEVL 106
Query: 113 LRLLEYLVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLF--RDPLGVRPLYYSS-- 168
L R K E A K LRG +A + L+ RDP G++PLYY++
Sbjct: 107 LATY------RHYKEEAASK-----LRGMFAFLIWNKNDHVLYGARDPFGIKPLYYTTIN 155
Query: 169 -RGHFASEKKVLWGLGEDAE------------PVLPGEIVSISKHGITRVRGFNVFSIRT 215
+ +FASE+K L D E +P E ++ H + +V + F+IR
Sbjct: 156 DQVYFASERKSLMVAQNDIEIDKEALQQYMSFQFVP-EPSTLDAH-VKKVEPGSQFTIRP 213
Query: 216 QG----KDYFNG---------------LQNLLIDSVKV--RASKKVGVLFSGGVDSSLIA 254
G K YF +++ + DSV V R+ VG SGG+DSS I
Sbjct: 214 DGDITFKTYFKANFKPVQTEEDKLVKEVRDAIYDSVNVHMRSDVPVGSFLSGGIDSSFIV 273
Query: 255 LIASRYSDVI-LYTSGTEDS--KDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAI 311
+A + + ++ G E +V+ A+ A LG++ + S E+ NE+ +I++
Sbjct: 274 SVAKEFHPSLKTFSVGFEQQGFSEVDVAKETAAALGIENISKVISPEEYMNELPKIVWHF 333
Query: 312 EEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGYAKYLQNPGLMLKDVEEL 370
++ P+ AIPLYF K A++ V V LSG+GADELFGGY Y + L LK E +
Sbjct: 334 DD--PLADPAAIPLYFVAKEAKKH-VTVALSGEGADELFGGYNIYRE--PLSLKPFERI 387
>gb|AAB95197.1| (U84118) asparagine synthetase [Aedes aegypti]
Length = 562
Score = 135 bits (337), Expect = 9e-31
Identities = 134/457 (29%), Positives = 207/457 (44%), Gaps = 66/457 (14%)
Query: 23 KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLAMTGSKSYTQPFYN---EII 79
+HRGPD G ++ E EG L+Q RL + G K+ TQPF + +++
Sbjct: 41 RHRGPDHTGLVAD-------------EDEG--FVLVQERLCVIGVKTGTQPFLSQDGQVL 85
Query: 80 LVHNGEIYNHESLRKYLIERGVAFET----DVDSEVILRLLEYLVFDRKLKVEEAIKLSM 135
LV NGEIYN+ + + + + A E D +VI+ E + + M
Sbjct: 86 LVANGEIYNYLLMAQEINDALKAGENMYTPRSDCDVIIAYYE----------KFGVDALM 135
Query: 136 KMLRGDYAVAFF--FNGKFYLFRDPLGVRPLYYSSRGH----FASEKKVLWGLGEDAEPV 189
K +RG +A + NG + RDP+G+ PLY ASE K L +
Sbjct: 136 KNIRGMFAFVLYDKKNGCILVARDPIGIVPLYSGKDAEGNLWIASEMKCLVETCPEVAIF 195
Query: 190 LPGEIVSISKHGITRVRGFN---VFSIRTQGKDYFNGLQNLLIDSVK--VRASKKVGVLF 244
PG + S+ + V F ++ I Q K L L D+V+ ++ +G L
Sbjct: 196 PPGHMYYGSREKLKSVSYFESDWMYEI-PQAKVDLERLNKSLEDAVESHLQCDVPMGALL 254
Query: 245 SGGVDSSLIALIASRYSD--------VILYTSGTEDSKDVEWARRVAEELGLKLRESLFS 296
SGG+DSSLIA IA++ + Y+ G D E+AR VA+ +G E F+
Sbjct: 255 SGGLDSSLIASIATKIMKKRHGPDYRLKTYSVGLVRGPDFEFARMVADYIGSDHTEVYFT 314
Query: 297 REDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGYAKY 356
++ N I + E + + IPL ++ + +G+K++LSG+GADELFGGY +
Sbjct: 315 IDEGLNYIRDAVLHGETYDITTVRCTIPLLLLSRYIKSEGIKMVLSGEGADELFGGYLYF 374
Query: 357 LQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRYPYLDLPFAILALNVPLELK 410
Q P +K V+ L + R +K G+E R P+LD F +++ E +
Sbjct: 375 HQAPNAEEFHFETVKRVQNLHYADCLRANKGTSAWGLELRVPFLDTDFVNYVMSIRPEDR 434
Query: 411 IRDGK--------RKFILREIAKSLGLPEWVAEREKK 439
K K+ILRE LP+ V R+K+
Sbjct: 435 KPKNKINGLVQPMEKYILREAFAEDYLPQKVLWRQKE 471
>pir||T18441 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) C0395w
[similarity] - malaria parasite (Plasmodium falciparum)
>gi|3649765|emb|CAB11114.1| (Z98547) predicted using
hexExon; MAL3P3.14 (PFC0395w), Asparagine synthetase,
len: 611 aa; Similarity to many asparagine synthetases.
O.sativa asparagine synthetase (SW:ASNS_ORYSA) BLAST
Score: 1003, sum P(2) = 5.5e-165; 65% identity in 290 aa
overlap
Length = 610
Score = 135 bits (337), Expect = 9e-31
Identities = 147/548 (26%), Positives = 251/548 (44%), Gaps = 106/548 (19%)
Query: 1 MCLIAGGFNVS------REDAIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEGGE 54
MC I F+ S R A+ + + +HRGPD W+ + ++D +G
Sbjct: 1 MCGILAIFHSSIEKHRLRRKALNLSKILRHRGPD----WNGIVVEEND--------DGTT 48
Query: 55 IALIQCRLAMTGSKSYTQPFYN---EIILVHNGEIYNHESLRKYLIERGV-AFETDVDSE 110
L RLA+ S QP Y+ E+ L NGEIYNH LRK + E + ++ D
Sbjct: 49 NVLAHERLAIVDVLSGHQPLYDDEEEVCLTINGEIYNHLELRKLIKEENLNKLKSCSDCA 108
Query: 111 VILRLLEYLVFDRKLKVEEAIKLSMKMLRGDYA--VAFFFNGKFYLFRDPLGVRPLY--Y 166
VI L + ++ K+ ML G +A ++ N F+ FRDP+G+ PLY Y
Sbjct: 109 VIPNLFK--IYKEKIP---------SMLDGIFAGVISDKKNNTFFAFRDPIGICPLYIGY 157
Query: 167 SSRGH--FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNV--FSIRTQ---GKD 219
++ G F+SE K L PG +K+ VR +N +S+ K
Sbjct: 158 AADGSIWFSSEFKALKDNCIRYVIFPPGHYYKNNKNKGEFVRYYNPNWWSLNNSIPNNKV 217
Query: 220 YFNGLQNLLIDSVKVRASKKV--GVLFSGGVDSSLIALIASRYSDVI------------- 264
FN ++ L +V R V G+L SGG+DSS+IA I +++ +++
Sbjct: 218 DFNEIRIHLEKAVIKRLMGDVPFGILLSGGLDSSIIAAILAKHLNILDNKNGKSIQNGND 277
Query: 265 ------------------------------LYTSGTEDSKDVEWARRVAEELGLKLRESL 294
++ G + S D++ A+ VAE LG++ E
Sbjct: 278 KKSNNNGNESNNNNNNNNFNNNNSGPQKLRSFSIGLKGSPDLKAAKEVAEYLGIEHTEFY 337
Query: 295 FSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGYA 354
F+ E+ + + +++ IE + + + P+Y ++ + VK++LSG+G+DE+FGGY
Sbjct: 338 FTVEEGIDSLHDVIYHIETYDITTIRASTPMYILSRLIKSSCVKMVLSGEGSDEIFGGYL 397
Query: 355 KYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRYPYLDLPFAILALNVPLE 408
+ + P + + + +L ++ R +K M G+E R P+LD+ F + +N+ +
Sbjct: 398 YFHKAPNKEEFHRELQRKIHDLHYYDVLRANKSTMAFGIEARVPFLDIQFLNVVMNIDPQ 457
Query: 409 LKIRDGKR--KFILREIAKSLGLPEWVAEREKKAAQYGSNAQKI--LEKIAKSRGVKLKG 464
K+ + K+ILR+ + LPE + R+K+ G I L++ A+ K
Sbjct: 458 DKMCSNNKIEKYILRKAFEGY-LPEHILYRQKEQFSDGVGYNWIDGLKQYAE------KK 510
Query: 465 LADILFSK 472
++DI FS+
Sbjct: 511 ISDIQFSR 518
>pir||JW0071 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) - soybean
>gi|1305549|gb|AAC09952.1| (U55874) asparagine
synthetase [Glycine max]
Length = 579
Score = 135 bits (336), Expect = 1e-30
Identities = 133/475 (28%), Positives = 218/475 (45%), Gaps = 69/475 (14%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++++ L KHRGPD WS DN+ L
Sbjct: 7 VLGCSDSSQAKRVRVLELSRRLKHRGPD----WSGLHQY-GDNY------------LAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE +++ NGEIYNHE LRK L F T D +VI L E
Sbjct: 50 RLAIVDPASGDQPLFNEDKTVVVTVNGEIYNHEELRKQLPNH--TFRTGSDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G ++ A + F + RD +GV LY
Sbjct: 108 -----------EHGENFVDMLDGIFSFALLDTRDNSFIVARDAIGVTSLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFN-GLQNLLID 230
+SE K L E E PG + S + R FS Y L++
Sbjct: 157 ISSELKGLNDDCEHFESFPPGHLYSSKERAFRRWYNPPWFSEAIPSAPYDPLALRHAFEK 216
Query: 231 SVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKDVE 277
+V R V GVL SGG+DSSL+A + +RY + + + G E + D++
Sbjct: 217 AVVKRLMTDVPFGVLLSGGLDSSLVAAVTARYLAGTNAAKQWGTKLHSFCVGLEGAPDLK 276
Query: 278 WARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGV 337
A+ VA+ +G E ++ +D + IE +++ IE + + +IP++ +++ + GV
Sbjct: 277 AAKEVADYIGTVHHEFHYTVQDGIDAIEDVIYHIETYDVTTIRASIPMFLMSRKIKSLGV 336
Query: 338 KVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRY 391
K ++SG+G+DE+FGGY + + P + ++ L + + R +K G+E R
Sbjct: 337 KWVISGEGSDEIFGGYLYFHKAPNKDEFHQETCRKIKALHKYDCLRANKSTFAWGLEARV 396
Query: 392 PYLDLPFAILALNVPLELKI---RDGK-RKFILR---EIAKSLGLPEWVAEREKK 439
P+LD F +A+N+ E K+ +G+ K++LR + + LP+ + R+K+
Sbjct: 397 PFLDKDFIRVAMNIDPEYKMIKKEEGRIEKWVLRRAFDDEEHPYLPKHILYRQKE 451
>gb|AAC72837.1| (AF095453) asparagine synthetase [Arabidopsis thaliana]
Length = 578
Score = 134 bits (335), Expect = 2e-30
Identities = 128/440 (29%), Positives = 199/440 (45%), Gaps = 62/440 (14%)
Query: 4 IAGGFNVSREDAIKIINLGK---HRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ +II L + HRGPD WS + + L
Sbjct: 7 VLGCIDNSQAKRSRIIELSRRLRHRGPD----WSGLHCYE-------------DCYLAHE 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP YNE + + NGEIYNH+ LR+ L + F T D EVI L E
Sbjct: 50 RLAIIDPTSGDQPLYNEDKTVAVTVNGEIYNHKILREKL--KSHQFRTGSDCEVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G +A + F RD +G+ PLY
Sbjct: 108 -----------EHGEEFIDMLDGMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFN-GLQNLLID 230
FASE K L E PG I S + G+ R ++ + Y L+N
Sbjct: 157 FASEMKALSDDCEQFMSFPPGHIYSSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEK 216
Query: 231 SVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKDVE 277
+V R V GVL SGG+DSSL+A +A R+ S + + G + S D++
Sbjct: 217 AVIKRLMTDVPFGVLLSGGLDSSLVAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLK 276
Query: 278 WARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGV 337
R VA+ LG + E F+ +D + IE +++ IE + + + P++ +++ + GV
Sbjct: 277 AGREVADYLGTRHHEFQFTVQDGIDAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGV 336
Query: 338 KVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRY 391
K++LSG+G+DE+ GGY + + P + ++ L + + R +K GVE R
Sbjct: 337 KMVLSGEGSDEILGGYLYFHKAPNKKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARV 396
Query: 392 PYLDLPFAILALNVPLELKI 411
P+LD F +A+++ E K+
Sbjct: 397 PFLDKEFLNVAMSIDPEWKL 416
>dbj|BAA97313.1| (AB019236) asparagine synthetase [Arabidopsis thaliana]
Length = 578
Score = 134 bits (335), Expect = 2e-30
Identities = 128/440 (29%), Positives = 199/440 (45%), Gaps = 62/440 (14%)
Query: 4 IAGGFNVSREDAIKIINLGK---HRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ +II L + HRGPD WS + + L
Sbjct: 7 VLGCIDNSQAKRSRIIELSRRLRHRGPD----WSGLHCYE-------------DCYLAHE 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP YNE + + NGEIYNH+ LR+ L + F T D EVI L E
Sbjct: 50 RLAIIDPTSGDQPLYNEDKTVAVTVNGEIYNHKILREKL--KSHQFRTGSDCEVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G +A + F RD +G+ PLY
Sbjct: 108 -----------EHGEEFIDMLDGMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFN-GLQNLLID 230
FASE K L E PG I S + G+ R ++ + Y L+N
Sbjct: 157 FASEMKALSDDCEQFMSFPPGHIYSSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEK 216
Query: 231 SVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKDVE 277
+V R V GVL SGG+DSSL+A +A R+ S + + G + S D++
Sbjct: 217 AVIKRLMTDVPFGVLLSGGLDSSLVAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLK 276
Query: 278 WARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGV 337
R VA+ LG + E F+ +D + IE +++ IE + + + P++ +++ + GV
Sbjct: 277 AGREVADYLGTRHHEFQFTVQDGIDAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGV 336
Query: 338 KVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRY 391
K++LSG+G+DE+ GGY + + P + ++ L + + R +K GVE R
Sbjct: 337 KMVLSGEGSDEILGGYLYFHKAPNKKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARV 396
Query: 392 PYLDLPFAILALNVPLELKI 411
P+LD F +A+++ E K+
Sbjct: 397 PFLDKEFLNVAMSIDPEWKL 416
>sp|P31752|ASNS_ASPOF ASPARAGINE SYNTHASE [GLUTAMINE-HYDROLYZING] (AS)
>gi|100980|pir||S25165 asparagine synthase
(glutamine-hydrolyzing) (EC 6.3.5.4) - garden asparagus
>gi|16076|emb|CAA48141.1| (X67958) asparagine synthase
(glutamine-hydrolysing) [Asparagus officinalis]
Length = 590
Score = 134 bits (333), Expect = 3e-30
Identities = 125/440 (28%), Positives = 197/440 (44%), Gaps = 62/440 (14%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++++ L KHRGPD G + G+ L
Sbjct: 7 VLGCSDDSQAKRVRVLELSRRLKHRGPDWSGL-----------------CQHGDCFLSHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP YNE I++ NGEIYNHE LR+ L + + T D EVI L E
Sbjct: 50 RLAIIDPASGDQPLYNEDKSIVVTVNGEIYNHEELRRRLPDH--KYRTGSDCEVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G ++ N F RD +G+ PLY
Sbjct: 108 -----------EHGEDFVDMLDGMFSFVLLDTRNNCFVAARDAVGITPLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFN-GLQNLLID 230
+SE K L E E PG + S R ++ Y L+ D
Sbjct: 157 LSSEMKGLNDDCEHFEVFPPGNLYSSRSGSFRRWYNPQWYNETIPSAPYDPLVLRKAFED 216
Query: 231 SVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKDVE 277
+V R V GVL SGG+DSSL+A + +R+ + + + G E S D++
Sbjct: 217 AVIKRLMTDVPFGVLLSGGLDSSLVAAVTARHLAGSKAAEQWGTQLHSFCVGLEGSPDLK 276
Query: 278 WARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGV 337
A+ VAE LG E F+ +D + IE ++F IE + + + P++ ++ + GV
Sbjct: 277 AAKEVAEYLGTVHHEFHFTVQDGIDAIEDVIFHIETYDVTTIRASTPMFLMARKIKSLGV 336
Query: 338 KVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRY 391
K+++SG+G+DE+FGGY + + P + ++ L + + R +K G+E R
Sbjct: 337 KMVISGEGSDEIFGGYLYFHKAPNKEEFHHETCRKIKALHQYDCLRANKATSAWGLEARV 396
Query: 392 PYLDLPFAILALNVPLELKI 411
P+LD F +A+++ E K+
Sbjct: 397 PFLDKEFMDVAMSIDPESKM 416
>emb|CAA67889.1| (X99552) asparagine synthetase [Asparagus officinalis]
Length = 590
Score = 134 bits (333), Expect = 3e-30
Identities = 125/440 (28%), Positives = 197/440 (44%), Gaps = 62/440 (14%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++++ L KHRGPD G + G+ L
Sbjct: 7 VLGCSDDSQAKRVRVLELSRRLKHRGPDWSGL-----------------CQHGDCFLSHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP YNE I++ NGEIYNHE LR+ L + + T D EVI L E
Sbjct: 50 RLAIIDPASGDQPLYNEDKSIVVTVNGEIYNHEELRRRLPDH--KYRTGSDCEVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G ++ N F RD +G+ PLY
Sbjct: 108 -----------EHGEDFVDMLDGMFSFVLLDTRNNCFVAARDAVGITPLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFN-GLQNLLID 230
+SE K L E E PG + S R ++ Y L+ D
Sbjct: 157 LSSEMKGLNDDCEHFEVFPPGNLYSSRSGSFRRWYNPQWYNETIPSAPYDPLVLRKAFED 216
Query: 231 SVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKDVE 277
+V R V GVL SGG+DSSL+A + +R+ + + + G E S D++
Sbjct: 217 AVIKRLMTDVPFGVLLSGGLDSSLVAAVTARHLAGSKAAEQWGTQLHSFCVGLEGSPDLK 276
Query: 278 WARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGV 337
A+ VAE LG E F+ +D + IE ++F IE + + + P++ ++ + GV
Sbjct: 277 AAKEVAEYLGTVHHEFHFTVQDGIDAIEDVIFHIETYDVTTIRASTPMFLMARKIKSLGV 336
Query: 338 KVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRY 391
K+++SG+G+DE+FGGY + + P + ++ L + + R +K G+E R
Sbjct: 337 KMVISGEGSDEIFGGYLYFHKAPNKEEFHHETCRKIKALHQYDCLRANKATSAWGLEARV 396
Query: 392 PYLDLPFAILALNVPLELKI 411
P+LD F +A+++ E K+
Sbjct: 397 PFLDKEFMDVAMSIDPESKM 416
>dbj|BAA96251.1| (AB035247) asparagine synthetase [Astragalus sinicus]
Length = 584
Score = 134 bits (333), Expect = 3e-30
Identities = 126/455 (27%), Positives = 201/455 (43%), Gaps = 66/455 (14%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + SR ++++ L KHRGPD WS + G+ L
Sbjct: 7 VLGCADASRAKRVRVLELSRRLKHRGPD----WSGLH-------------QHGDCYLAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE +I+ NGEIYNHE LRK L F T D +VI L E
Sbjct: 50 RLAIVDPASGDQPLFNEDKSVIVTVNGEIYNHEDLRKQLPNH--TFRTGSDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
D + ML G ++ + + + RD +GV LY
Sbjct: 108 EYGED-----------FVDMLDGIFSFVLLDTRDNSYIVARDAIGVTSLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFNGLQNLLIDS 231
+SE K L E E PG + S G R +S Y + +
Sbjct: 157 ISSEMKGLNDDCEHFECFPPGHLYSSKNGGFRRWYNPLWYSEAIPSAPYDPIVLRQAFEK 216
Query: 232 VKVR---ASKKVGVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKDVE 277
++ GVL SGG+DSSL+A I SRY S + + G E S D++
Sbjct: 217 AVIKRLMTDVPFGVLLSGGLDSSLVASITSRYLATTKAAEQWGSKLHSFCVGLEGSPDLK 276
Query: 278 WARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGV 337
+ VA+ LG E F+ +D + IE +++ +E + + + P++ +++ + GV
Sbjct: 277 AGKEVADYLGTVHHEFTFTVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGV 336
Query: 338 KVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRY 391
K ++SG+G+DE+FGGY + + P + ++ L + + R +K G+E R
Sbjct: 337 KWVISGEGSDEIFGGYLYFHKAPNKEEFHRETCRKIKALYQYDCLRANKSTFAWGLEARV 396
Query: 392 PYLDLPFAILALNVP---LELKIRDGK-RKFILRE 422
P+LD F +A+N+ + + +G+ K+ILR+
Sbjct: 397 PFLDKEFINVAMNIDPANKMINLDEGRIEKYILRK 431
>gb|AAB81011.1| (U89923) asparagine synthetase [Medicago sativa]
Length = 586
Score = 133 bits (331), Expect = 5e-30
Identities = 138/477 (28%), Positives = 214/477 (43%), Gaps = 72/477 (15%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++I+ L KHRGPD G DN+ L
Sbjct: 7 VLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQH-----GDNY------------LAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE II+ NGEIYNHE LRK L F T D +VI L E
Sbjct: 50 RLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLPNH--KFRTQCDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G ++ + F + RD +GV LY
Sbjct: 108 -----------EYGENFVDMLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRG---FNVFSIRTQGKDYFNGLQNLL 228
ASE K L E E PG + S R FN I T D L+N
Sbjct: 157 IASELKGLNDECEHFEVFPPGHLYSSKDREFRRWYNPPWFNEAVIPTTPYDPLV-LRNAF 215
Query: 229 IDSVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKD 275
+V R V GVL SGG+DSSL+A + +RY + + + G + + D
Sbjct: 216 EKAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARYLAGTKAAKQWGAKLHSFCVGLKGAPD 275
Query: 276 VEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRARED 335
++ R VA+ LG E F+ +D + IE +++ E + + A P++ +++ +
Sbjct: 276 LKAGREVADFLGTVHHEFQFTIQDGIDAIEDVIYHTETYDVTTIRAATPMFLMSRKIKSS 335
Query: 336 GVKVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVET 389
GVK ++SG+G+DE+FGGY + + P + ++ L + R +K G+E
Sbjct: 336 GVKWVISGEGSDEIFGGYLYFHKAPNKEEFHQETCRKIKALHRYDCLRANKSTYAWGLEA 395
Query: 390 RYPYLDLPFAILALNVPLELKI---RDGK-RKFILREI---AKSLGLPEWVAEREKK 439
R P+LD F +A+++ E K+ +G+ K+ILR+ ++ LP+ + R+K+
Sbjct: 396 RVPFLDKDFIKVAMDIDPEYKMIKHEEGRIEKWILRKAFDDEENPYLPKHILYRQKE 452
>gb|AAB48058.1| (L40327) asparagine synthetase [Medicago sativa]
Length = 586
Score = 133 bits (331), Expect = 5e-30
Identities = 138/477 (28%), Positives = 214/477 (43%), Gaps = 72/477 (15%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++I+ L KHRGPD G DN+ L
Sbjct: 7 VLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQH-----GDNY------------LAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE II+ NGEIYNHE LRK L F T D +VI L E
Sbjct: 50 RLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEDLRKQLPNH--KFRTQCDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G ++ + F + RD +GV LY
Sbjct: 108 -----------EYGENFVDMLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRG---FNVFSIRTQGKDYFNGLQNLL 228
ASE K L E E PG + S R FN I T D L+N
Sbjct: 157 IASELKGLNDECEHFEVFPPGHLYSSKDREFRRWYNPPWFNEAVIPTTPYDPLV-LRNAF 215
Query: 229 IDSVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKD 275
+V R V GVL SGG+DSSL+A + +RY + + + G + + D
Sbjct: 216 EKAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARYLAGTKAAKQWGAKLHSFCVGLKGAPD 275
Query: 276 VEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRARED 335
++ R VA+ LG E F+ +D + IE +++ E + + A P++ +++ +
Sbjct: 276 LKAGREVADFLGTVHHEFQFTIQDGIDAIEDVIYHTETYDVTTIRAATPMFLMSRKIKSS 335
Query: 336 GVKVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVET 389
GVK ++SG+G+DE+FGGY + + P + ++ L + R +K G+E
Sbjct: 336 GVKWVISGEGSDEIFGGYLYFHKAPNKEEFHQETCRKIKALHRYDCLRANKSTYAWGLEA 395
Query: 390 RYPYLDLPFAILALNVPLELKI---RDGK-RKFILREI---AKSLGLPEWVAEREKK 439
R P+LD F +A+++ E K+ +G+ K+ILR+ ++ LP+ + R+K+
Sbjct: 396 RVPFLDKEFIKVAMDIDPEYKMIKHEEGRIEKWILRKAFDDEENPYLPKHILYRQKE 452
>sp|O24661|ASNS_TRIVS ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|2429280|gb|AAD05033.1|
(AF014055) asparagine synthetase [Triphysaria
versicolor] >gi|2429282|gb|AAD05034.1| (AF014056)
asparagine synthetase [Triphysaria versicolor]
>gi|2429284|gb|AAD05035.1| (AF014057) asparagine
synthetase [Triphysaria versicolor]
Length = 586
Score = 132 bits (330), Expect = 6e-30
Identities = 132/475 (27%), Positives = 210/475 (43%), Gaps = 69/475 (14%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++++ L KHRGPD G G+ L
Sbjct: 7 VLGCSDDSQAKRVRVLELSRRLKHRGPDWSGIHHH-----------------GDCYLAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE I + NGEIYNHE LR L F T D +VI L E
Sbjct: 50 RLAIVDPASGDQPLFNEDKRIAVTVNGEIYNHEELRALLPNH--KFRTGSDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFFN--GKFYLFRDPLGVRPLYYS----SRGH 171
E + ++ML G ++ + F RD G+ LY
Sbjct: 108 -----------EYGENFVEMLDGMFSFVLLDSRDNTFIAARDAFGITSLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFN-GLQNLLID 230
+SE K L E+ E PG + S G R FS Y L+
Sbjct: 157 ISSELKGLHDECENFEVFPPGHVYSSKTEGFRRWYNPPWFSEAIPSTPYDPLVLRGAFEQ 216
Query: 231 SVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKDVE 277
+V R V GVL SGG+DSSL+A + +R+ S + + G E S D++
Sbjct: 217 AVIKRLMTDVPFGVLLSGGLDSSLVAAVTARHLAGTKAAKRWGSQLHSFCVGLEGSPDLK 276
Query: 278 WARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGV 337
+ VA+ LG E LF+ +D + IE +++ IE + + + P++ +++ + GV
Sbjct: 277 AGKEVADYLGTVHHEFLFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGV 336
Query: 338 KVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRY 391
K+++SG+G+DE+FGGY + + P + ++ L + + R +K G+E R
Sbjct: 337 KMVISGEGSDEIFGGYLYFHKAPNKEEFHRETCRKIKALHQYDCLRANKATSAWGLEARV 396
Query: 392 PYLDLPFAILALNVPLELKI----RDGKRKFILREI---AKSLGLPEWVAEREKK 439
P+LD F LA+++ E K+ + K+ILR+ + LP+ + R+K+
Sbjct: 397 PFLDKEFVNLAMSIDPEAKMIKPDQGRIEKWILRKAFDDEERPYLPKHILYRQKE 451
>dbj|BAA96252.1| (AB035248) asparagine synthetase [Astragalus sinicus]
Length = 586
Score = 132 bits (330), Expect = 6e-30
Identities = 133/475 (28%), Positives = 211/475 (44%), Gaps = 69/475 (14%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++I+ L KHRGPD G DN+ L
Sbjct: 7 VLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQH-----GDNY------------LAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +N+ II+ NGEIYNHE LRK L F TD D +VI L E
Sbjct: 50 RLAIVDPASGDQPLFNQEKSIIVTVNGEIYNHEELRKQLPNH--KFRTDCDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRG--DYAVAFFFNGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G + V + F + RD +GV LY
Sbjct: 108 -----------EYGENFVDMLDGIFSFVVLDTRDNSFLVARDAIGVTSLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFNGLQNLLIDS 231
ASE K L E E PG + S + R FS Y + +
Sbjct: 157 IASELKGLNDECEHFEVFPPGHLYSSKEREFRRWYNPKWFSETVPSSPYDPLVLRHAFEK 216
Query: 232 VKVR---ASKKVGVLFSGGVDSSLIALIASRYSD-----------VILYTSGTEDSKDVE 277
++ + GVL SGG+DSSL+A + +RY + + + G + + D++
Sbjct: 217 AVIKRLMSDVPFGVLLSGGLDSSLVASVTARYLEDTKAAKQWGAKLHTFCVGLKGAPDLK 276
Query: 278 WARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGV 337
R VA+ LG E F+ +D + IE +++ E + + + P++ +++ + GV
Sbjct: 277 AGREVADYLGTVHHEFEFTIQDGIDAIEDVIYHTETFDVTTIRASTPMFLMSRKIKSLGV 336
Query: 338 KVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRY 391
K++LSG+G+DE+FGGY + + P + ++ L + + R +K G+E R
Sbjct: 337 KMVLSGEGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALYKYDCLRANKSTYAWGLEARV 396
Query: 392 PYLDLPFAILALNVPLELKI--RDGKR--KFILREIAKSLG---LPEWVAEREKK 439
P+LD F +A+++ E K+ RD R K++LR+ LP + R+K+
Sbjct: 397 PFLDKDFINVAMDIDPEEKMIKRDEGRIEKWVLRKAFDDENKPYLPRHILYRQKE 451
>sp|P49078|ASNS_ARATH ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|7438081|pir||T12989
asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
- Arabidopsis thaliana >gi|507946|gb|AAA74359.1|
(L29083) glutamine-dependent asparagine synthetase
[Arabidopsis thaliana] >gi|5541701|emb|CAB51206.1|
(AL096860) glutamine-dependent asparagine synthetase
[Arabidopsis thaliana]
Length = 584
Score = 132 bits (329), Expect = 8e-30
Identities = 131/477 (27%), Positives = 212/477 (43%), Gaps = 73/477 (15%)
Query: 4 IAGGFNVSREDAIKIINLGK---HRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++++ L + HRGPD G + + G+ L
Sbjct: 7 VLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLY-----------------QNGDNYLAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE I++ NGEIYNHE LRK L + F T D EVI L E
Sbjct: 50 RLAVIDPASGDQPLFNEDKTIVVTVNGEIYNHEELRKRL--KNHKFRTGSDCEVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
D + ML G ++ + F + RD +GV LY
Sbjct: 108 EYGVD-----------FVDMLDGIFSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYF-----NGLQN 226
+SE K L E E PG S G + F+ Y +N
Sbjct: 157 ISSEMKGLNDDCEHFETFPPGHFYSSKLGGFKQWYNPPWFNESVPSTPYEPLAIRRAFEN 216
Query: 227 LLIDSVKVRASKKVGVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKD 275
+I ++ GVL SGG+DSSL+A I +R+ + + G E S D
Sbjct: 217 AVIK--RLMTDVPFGVLLSGGLDSSLVASITARHLAGTKAAKQWGPQLHSFCVGLEGSPD 274
Query: 276 VEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRARED 335
++ + VAE LG E FS +D + IE +++ +E + + + P++ +++ +
Sbjct: 275 LKAGKEVAEYLGTVHHEFHFSVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSL 334
Query: 336 GVKVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVET 389
GVK++LSG+GADE+FGGY + + P + ++ L + + R +K G+E
Sbjct: 335 GVKMVLSGEGADEIFGGYLYFHKAPNKKEFHQETCRKIKALHKYDCLRANKSTSAFGLEA 394
Query: 390 RYPYLDLPFAILALNVPLE---LKIRDGK-RKFILR---EIAKSLGLPEWVAEREKK 439
R P+LD F A+++ E +K +G+ K++LR + + LP+ + R+K+
Sbjct: 395 RVPFLDKDFINTAMSLDPESKMIKPEEGRIEKWVLRRAFDDEERPYLPKHILYRQKE 451
>emb|CAB57292.1| (AJ133522) asparagine synthetase (type-I) [Phaseolus vulgaris]
Length = 579
Score = 132 bits (328), Expect = 1e-29
Identities = 134/475 (28%), Positives = 214/475 (44%), Gaps = 69/475 (14%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++++ L KHRGPD WS G+ L
Sbjct: 7 VLGCSDSSQAKRVRVLELSRRLKHRGPD----WSGLHQY-------------GDCYLAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE I + NGEIYNHE LRK L F T D +VI L E
Sbjct: 50 RLAIVDPASGDQPLFNEDKTIAVTVNGEIYNHEELRKQLPNH--TFRTGSDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G ++ + F + RD +GV LY
Sbjct: 108 -----------EHGENFVDMLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFN-GLQNLLID 230
+SE K L G E E PG + S + R FS Y L++
Sbjct: 157 ISSELKGLNGDCEHFESFPPGHLYSSKEREFRRWYNPPWFSEAIPSAPYDPLALRHAFEK 216
Query: 231 SVKVRASKKV--GVLFSGGVDSSLIALIASRYSD-----------VILYTSGTEDSKDVE 277
+V R V GVL SGG+DSSL+A + +RY + + + G E + D++
Sbjct: 217 AVIKRLMTDVPFGVLLSGGLDSSLVAAVTARYLEGTNAAKHWGTKLHSFCVGLEGAPDLK 276
Query: 278 WARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGV 337
AR VA+ +G E +S +D + IE +++ IE + + +IP++ +++ + GV
Sbjct: 277 AAREVADYIGTVHHEFHYSVQDGIDAIEDVIYHIETYDVTTIRASIPMFLMSRKIKSLGV 336
Query: 338 KVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRY 391
K + G+G+DE++GGY + + P + ++ L + + R +K G+E R
Sbjct: 337 KWDICGEGSDEIYGGYLYFHKAPNKEEFHQETCRKIKALHKYDCLRANKSTFAWGLEARV 396
Query: 392 PYLDLPFAILALNVPLELKI---RDGK-RKFILR---EIAKSLGLPEWVAEREKK 439
P+LD F +A+NV E K+ +G+ K++LR + + LP+ + R+K+
Sbjct: 397 PFLDKEFIEVAMNVNPEYKMIKKEEGRIEKWVLRRAFDDGEHPYLPKHILYRQKE 451
>gb|AAF74755.1| (AF263432) asparagine synthetase [Helianthus annuus]
Length = 589
Score = 132 bits (328), Expect = 1e-29
Identities = 126/453 (27%), Positives = 206/453 (44%), Gaps = 66/453 (14%)
Query: 23 KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLAMTGSKSYTQPFYNE---II 79
KHRGPD G + DN+ L RLA+ S QP YNE ++
Sbjct: 29 KHRGPDWSGLYQH-----GDNY------------LSHQRLAVIDPASGDQPLYNEDETVV 71
Query: 80 LVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLR 139
+ NGEIYNH LR+ L+ G F+T D +VI L E E + + ML
Sbjct: 72 VTVNGEIYNHMELREKLM--GHKFKTGSDCDVIAHLYE-----------EHGENFVDMLD 118
Query: 140 GDYAVAFFF--NGKFYLFRDPLGVRPLYYS-SRGHFASEKKVLWGLGEDAEPV---LPGE 193
G ++ + + + RD +G+ LY L GL +D E L G
Sbjct: 119 GMFSFVLLGTRDNSYLVARDAIGITSLYIGWGLDGSVLISSGLKGLNDDCEHFEVFLAGH 178
Query: 194 IVSISKHGITRVRGFNVFSIRTQGKDYFNGLQNLLIDSVKVR---ASKKVGVLFSGGVDS 250
+ S G R FS Y + ++ ++ GVL SGG+DS
Sbjct: 179 LYSSKIRGFRRWYNLPWFSESIPSTPYDPLVLRCAFENAVIKRLMTDVPFGVLLSGGLDS 238
Query: 251 SLIALIASRY-----------SDVILYTSGTEDSKDVEWARRVAEELGLKLRESLFSRED 299
SL+A + +R+ + + + G E S D++ AR VA+ LG E F+ +D
Sbjct: 239 SLVASVTARHLAGSKAAKQWGAQLHSFCVGLEGSPDLKAAREVADYLGTLHHEFHFTVQD 298
Query: 300 IENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGYAKYLQN 359
+ IE +++ IE + + + P++ +++ + GVK+++SG+G+DE+FGGY + +
Sbjct: 299 GIDAIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYFHKA 358
Query: 360 PGL------MLKDVEELGERNLARDDKVAMLNGVETRYPYLDLPFAILALNVPLELKI-- 411
P + ++ L + + R +K G+E R P+LD F +A+++ E K+
Sbjct: 359 PNKEEFHQETCRKIKALHQYDCLRANKSTSAWGLEARVPFLDKEFINVAMSIDPEAKMIN 418
Query: 412 RDGKR--KFILR---EIAKSLGLPEWVAEREKK 439
D KR K+ILR + + LP+ + R+K+
Sbjct: 419 MDQKRIEKWILRRAFDDEEKPYLPKHILYRQKE 451
>gi|11421677 asparagine synthetase [Homo sapiens] >gi|3341715|gb|AAC62263.1|
(AC005326) asparagine synthetase [Homo sapiens]
Length = 561
Score = 131 bits (327), Expect = 1e-29
Identities = 137/488 (28%), Positives = 229/488 (46%), Gaps = 97/488 (19%)
Query: 24 HRGPDSFGFWSERQMLKSDNFSDVSEIEGG--EIALIQCRLAMTGSKSYTQPFYNEIILV 81
HRGPD+F F +N + + G +A++ M + P+ + L
Sbjct: 26 HRGPDAFRF---------ENVNGYTNCCFGFHRLAVVDPLFGMQPIRVKKYPY---LWLC 73
Query: 82 HNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGD 141
+NGEIYNH+ ++++ ++T VD E+IL L + + I+ ++ ML G
Sbjct: 74 YNGEIYNHKKMQQHF---EFEYQTKVDGEIILHLYD----------KGGIEQTICMLDGV 120
Query: 142 YAVAFF--FNGKFYLFRDPLGVRPLY--YSSRGHFA--SEKKVLWGLGEDA------EPV 189
+A N K +L RD GVRPL+ + G A SE K L L A EP
Sbjct: 121 FAFVLLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVTLKHSATPFLKVEPF 180
Query: 190 LPG--EIVSISKHG----ITRVR---------------------GFNVFSIRTQGKDYFN 222
LPG E++ + +G + V+ GF + +++ N
Sbjct: 181 LPGHYEVLDLKPNGKVASVEMVKYHHCRDVPLHALYDNVEKLFPGFEIETVK-------N 233
Query: 223 GLQNLLIDSVKVR--ASKKVGVLFSGGVDSSLIALI-------ASRYSDVILYTSGTEDS 273
L+ L ++VK R +++G L SGG+DSSL+A A + + G EDS
Sbjct: 234 NLRILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKEAQVQYPLQTFAIGMEDS 293
Query: 274 KDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTK--R 331
D+ AR+VA+ +G + E LF+ E+ ++ ++F++E + + ++ +Y +K R
Sbjct: 294 PDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETYDITTVRASVGMYLISKYIR 353
Query: 332 AREDGVKVLLSGQGADELFGGYAKYLQNPGLMLKDVEELGER--------NLARDDKVAM 383
D V V+ SG+G+DEL GY + + P + EE ER ++ R D+
Sbjct: 354 KNTDSV-VIFSGEGSDELTQGYIYFHKAPS--PEKAEEESERLLRELYLFDVLRADRTTA 410
Query: 384 LNGVETRYPYLDLPFAILALNVPLELKI-RDGKRKFILREIAKSLGL-PEWVAEREKKAA 441
+G+E R P+LD F+ L++P E++I ++G K +LRE + L P+ + R K+A
Sbjct: 411 AHGLELRVPFLDHRFSSYYLSLPPEMRIPKNGIEKHLLRETFEDSNLIPKEILWRPKEAF 470
Query: 442 QYGSNAQK 449
G + K
Sbjct: 471 SDGITSVK 478
>gb|AAC16325.1| (AF061740) asparagine synthetase [Elaeagnus umbellata]
Length = 585
Score = 131 bits (327), Expect = 1e-29
Identities = 134/468 (28%), Positives = 211/468 (44%), Gaps = 69/468 (14%)
Query: 11 SREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLAMTGS 67
S+ ++++ L KHRGPD G + + G+ L RLA+
Sbjct: 14 SQAKRVRVLELSRRLKHRGPDWSGLF-----------------QFGDCYLAHQRLAIIDP 56
Query: 68 KSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRK 124
S QP +NE +++ NGEIYNHE LRK L F T D +VI L E D
Sbjct: 57 ASGDQPLFNEDKTVVVTVNGEIYNHEELRKKLSHH--KFRTGSDCDVIAHLYEEYGED-- 112
Query: 125 LKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGHFASEKKV 178
+ ML G +A + F + RD +GV LY +SE K
Sbjct: 113 ---------FVDMLDGIFAFVLLDTRDNSFIVARDAIGVTSLYIGWGLDGSIWISSELKG 163
Query: 179 LWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFN-GLQNLLIDSVKVRAS 237
L E E PG + S G+ R FS Y L+ ++V R
Sbjct: 164 LNNDCEHFETFPPGHLYSSKAGGMRRWYNPVWFSETIPSAPYDPLALRRAFENAVIKRLM 223
Query: 238 KKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKDVEWARRVAE 284
V GVL SGG+DSSL+A I +R+ + + + G E S D++ A+ VA
Sbjct: 224 TDVPFGVLLSGGLDSSLVASITARHLSGTKAAKQWGTKLHSFCVGLEGSPDLKAAKEVAA 283
Query: 285 ELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQ 344
LG E F+ +D + IE +++ +E + + + P++ +++ + GVK+++SG+
Sbjct: 284 YLGTVHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVMSGE 343
Query: 345 GADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRYPYLDLPF 398
G+DE+FGGY + + P + ++ L + R +K G+E R P+LD F
Sbjct: 344 GSDEIFGGYLYFHKAPNKEEFHEETCRKIKALYLYDCLRANKSTTAWGLEARVPFLDKEF 403
Query: 399 AILALNVPLELK--IRDGKR--KFILREI---AKSLGLPEWVAEREKK 439
+A+N+ E K RD R K++LR+ + LP+ + R+K+
Sbjct: 404 INVAMNIDPEYKKIKRDEGRIEKWVLRKAFDDEERPYLPKHILYRQKE 451
>prf||2207183A Asn synthetase [Rattus norvegicus]
Length = 561
Score = 131 bits (326), Expect = 2e-29
Identities = 138/488 (28%), Positives = 229/488 (46%), Gaps = 97/488 (19%)
Query: 24 HRGPDSFGFWSERQMLKSDNFSDVSEIEGG--EIALIQCRLAMTGSKSYTQPFYNEIILV 81
HRGPD+F F +N + + G +A++ M + P+ + L
Sbjct: 26 HRGPDAFRF---------ENVNGYTNCCFGFHRLAVVDPLFGMQPIRVRKYPY---LWLC 73
Query: 82 HNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGD 141
+NGEIYNH++L++ ++T+VD E+IL L + + I+ ++ ML G
Sbjct: 74 YNGEIYNHKALQQRF---EFEYQTNVDGEIILHLYD----------KGGIEKTICMLDGV 120
Query: 142 YAVAFF--FNGKFYLFRDPLGVRPLY--YSSRGHFA--SEKKVLWGLGED------AEPV 189
+A N K +L RD GVRPL+ + G A SE K L L EP
Sbjct: 121 FAFILLDTANKKVFLGRDTYGVRPLFKALTEDGFLAVCSEAKGLVSLKHSTTPFLKVEPF 180
Query: 190 LPG--EIVSISKHG----ITRVR---------------------GFNVFSIRTQGKDYFN 222
LPG E++ + +G + V+ GF + +++ N
Sbjct: 181 LPGHYEVLDLKPNGKVASVEMVKYHHCTDEPLHAIYDSVEKLFPGFEIETVK-------N 233
Query: 223 GLQNLLIDSVKVR--ASKKVGVLFSGGVDSSLIA--LIASRYSDVILY-----TSGTEDS 273
L+ L +++K R +++G L SGG+DSSL+A L+ + Y G EDS
Sbjct: 234 NLRILFNNAIKKRLMTDRRIGCLLSGGLDSSLVAASLLKQLKEAQVPYALQTFAIGMEDS 293
Query: 274 KDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTK--R 331
D+ AR+VA +G + E LF+ E+ ++ ++F++E + + ++ +Y +K R
Sbjct: 294 PDLLAARKVANYIGSEHHEVLFNSEEGIQSLDEVIFSLETYDITTVRASVGMYLISKYIR 353
Query: 332 AREDGVKVLLSGQGADELFGGYAKYLQNPGLMLKDVEELGER--------NLARDDKVAM 383
D V V+ SG+G+DEL GY + + P + EE ER ++ R D+
Sbjct: 354 KNTDSV-VIFSGEGSDELTQGYIYFHKAPS--PEKAEEESERLLKELYLFDVLRADRTTA 410
Query: 384 LNGVETRYPYLDLPFAILALNVPLELKI-RDGKRKFILREIAKSLG-LPEWVAEREKKAA 441
+G+E R P+LD F+ L++P E++I +DG K +LRE + LP+ + R K+A
Sbjct: 411 AHGLELRVPFLDHRFSSYYLSLPPEMRIPKDGIEKHLLRETFEDSNLLPKEILWRPKEAF 470
Query: 442 QYGSNAQK 449
G + K
Sbjct: 471 SDGITSVK 478
>sp|P49088|ASNS_RAT ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|1083600|pir||S53447
asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
[validated] - rat >gi|460631|gb|AAA77671.1| (U07201)
asparagine synthetase [Rattus norvegicus]
>gi|460633|gb|AAA77672.1| (U07202) asparagine synthetase
[Rattus norvegicus]
Length = 561
Score = 130 bits (324), Expect = 3e-29
Identities = 138/488 (28%), Positives = 228/488 (46%), Gaps = 97/488 (19%)
Query: 24 HRGPDSFGFWSERQMLKSDNFSDVSEIEGG--EIALIQCRLAMTGSKSYTQPFYNEIILV 81
HRGPD+F F +N + + G +A++ M + P+ + L
Sbjct: 26 HRGPDAFRF---------ENVNGYTNCCFGFHRLAVVDPLFGMQPIRVRKYPY---LWLC 73
Query: 82 HNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGD 141
+NGEIYNH++L++ ++T+VD E+IL L + + I+ ++ ML G
Sbjct: 74 YNGEIYNHKALQQRF---EFEYQTNVDGEIILHLYD----------KGGIEKTICMLDGV 120
Query: 142 YAVAFF--FNGKFYLFRDPLGVRPLY--YSSRGHFA--SEKKVLWGLGED------AEPV 189
+A N K +L RD GVRPL+ + G A SE K L L EP
Sbjct: 121 FAFILLDTANKKVFLGRDTYGVRPLFKALTEDGFLAVCSEAKGLVSLKHSTTPFLKVEPF 180
Query: 190 LPG--EIVSISKHG----ITRVR---------------------GFNVFSIRTQGKDYFN 222
LPG E++ + +G + V+ GF + +++ N
Sbjct: 181 LPGHYEVLDLKPNGKVASVEMVKYHHCTDEPLHAIYDSVEKLFPGFEIETVK-------N 233
Query: 223 GLQNLLIDSVKVR--ASKKVGVLFSGGVDSSLIA--LIASRYSDVILY-----TSGTEDS 273
L+ L +++K R +++G L SGG+DSSL+A L+ + Y G EDS
Sbjct: 234 NLRILFNNAIKKRLMTDRRIGCLLSGGLDSSLVAASLLKQLKEAQVPYALQTFAIGMEDS 293
Query: 274 KDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTK--R 331
D+ AR+VA +G + E LF+ E+ ++ ++F +E + + ++ +Y +K R
Sbjct: 294 PDLLAARKVANYIGSEHHEVLFNSEEGIQSLDEVIFPLETYDITTVRASVGMYLISKYIR 353
Query: 332 AREDGVKVLLSGQGADELFGGYAKYLQNPGLMLKDVEELGER--------NLARDDKVAM 383
D V V+ SG+G+DEL GY + + P + EE ER ++ R D+
Sbjct: 354 KNTDSV-VIFSGEGSDELTQGYIYFHKAPS--PEKAEEESERLLKELYLFDVLRADRTTA 410
Query: 384 LNGVETRYPYLDLPFAILALNVPLELKI-RDGKRKFILREIAKSLG-LPEWVAEREKKAA 441
+G+E R P+LD F+ L++P E++I +DG K +LRE + LP+ + R K+A
Sbjct: 411 AHGLELRVPFLDHRFSSYYLSLPPEMRIPKDGIEKHLLRETFEDSNLLPKEILWRPKEAF 470
Query: 442 QYGSNAQK 449
G + K
Sbjct: 471 SDGITSVK 478
>gi|4502259 asparagine synthetase [Homo sapiens]
>gi|114266|sp|P08243|ASNS_HUMAN ASPARAGINE SYNTHETASE
[GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT ASPARAGINE
SYNTHETASE) (TS11 CELL CYCLE CONTROL PROTEIN)
>gi|68655|pir||AJHUN1 asparagine synthase
(glutamine-hydrolyzing) (EC 6.3.5.4) - human
>gi|179100|gb|AAA51789.1| (M27396) asparagine synthetase
[Homo sapiens] >gi|533200|gb|AAA52756.1| (L35946)
asparagine synthetase [Homo sapiens]
Length = 561
Score = 130 bits (324), Expect = 3e-29
Identities = 137/488 (28%), Positives = 228/488 (46%), Gaps = 97/488 (19%)
Query: 24 HRGPDSFGFWSERQMLKSDNFSDVSEIEGG--EIALIQCRLAMTGSKSYTQPFYNEIILV 81
HRGPD+F F +N + + G +A++ M + P+ + L
Sbjct: 26 HRGPDAFRF---------ENVNGYTNCCFGFHRLAVVDPLFGMQPIRVKKYPY---LWLC 73
Query: 82 HNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGD 141
+NGEIYNH+ ++++ ++T VD E+IL L + + I+ ++ ML G
Sbjct: 74 YNGEIYNHKKMQQHF---EFEYQTKVDGEIILHLYD----------KGGIEQTICMLDGV 120
Query: 142 YAVAFF--FNGKFYLFRDPLGVRPLY--YSSRGHFA--SEKKVLWGLGEDA------EPV 189
+A N K +L RD GVRPL+ + G A SE K L L A EP
Sbjct: 121 FAFVLLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVTLKHSATPFLKVEPF 180
Query: 190 LPG--EIVSISKHG----ITRVR---------------------GFNVFSIRTQGKDYFN 222
LPG E++ + +G + V+ GF + +++ N
Sbjct: 181 LPGHYEVLDLKPNGKVASVEMVKYHHCRDVPLHALYDNVEKLFPGFEIETVK-------N 233
Query: 223 GLQNLLIDSVKVR--ASKKVGVLFSGGVDSSLIALI-------ASRYSDVILYTSGTEDS 273
L+ L ++VK R +++G L SGG+DSSL+A A + + G EDS
Sbjct: 234 NLRILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKEAQVQYPLQTFAIGMEDS 293
Query: 274 KDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTK--R 331
D+ AR+VA+ +G + E LF+ E+ ++ ++F++E + + ++ +Y +K R
Sbjct: 294 PDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETYDITTVRASVGMYLISKYIR 353
Query: 332 AREDGVKVLLSGQGADELFGGYAKYLQNPGLMLKDVEELGER--------NLARDDKVAM 383
D V V+ SG+G+DEL GY + + P + EE ER ++ R D+
Sbjct: 354 KNTDSV-VIFSGEGSDELTQGYIYFHKAPS--PEKAEEESERLLRELYLFDVLRADRTTA 410
Query: 384 LNGVETRYPYLDLPFAILALNVPLELKI-RDGKRKFILREIAKSLGL-PEWVAEREKKAA 441
+G+E R P+LD F L++P E++I ++G K +LRE + L P+ + R K+A
Sbjct: 411 AHGLELRVPFLDHRFFSYYLSLPPEMRIPKNGIEKHLLRETFEDSNLIPKEILWRPKEAF 470
Query: 442 QYGSNAQK 449
G + K
Sbjct: 471 SDGITSVK 478
>sp|P49093|ASN2_LOTJA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 2
(GLUTAMINE-DEPENDENT ASPARAGINE SYNTHETASE 2)
>gi|2147132|pir||S57932 asparagine synthase
(glutamine-hydrolysing) (EC 6.3.5.4) - Lotus japonicus
>gi|2147134|pir||S69183 asparagine synthase
(glutamine-hydrolyzing) (EC 6.3.5.4) 2 - Lotus japonicus
>gi|897773|emb|CAA61590.1| (X89410) asparagine synthase
(glutamine-hydrolysing) [Lotus japonicus]
Length = 586
Score = 130 bits (324), Expect = 3e-29
Identities = 136/475 (28%), Positives = 214/475 (44%), Gaps = 69/475 (14%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++++ L KHRGPD G DNF L
Sbjct: 7 VLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLHQH-----GDNF------------LAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE II+ NGEI+NHE LRK L F T D +VI L E
Sbjct: 50 RLAIVDPASGDQPLFNEDQSIIVTVNGEIFNHEELRKQLPNH--KFRTGCDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLY--YSSRGH-- 171
E + + ML G ++ + F + RD +GV LY Y G
Sbjct: 108 -----------EHGENFVDMLDGIFSFVLLDTRDNSFLVARDAIGVTSLYIGYGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFN-GLQNLLID 230
ASE K L E E PG + S + R FS Y L+
Sbjct: 157 IASELKGLNDDCEHFELFPPGHLYSSKEKEFRRWYNPPWFSEAIPSAPYDPLALRQAFEK 216
Query: 231 SVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKDVE 277
++ R V GVL SGG+DSSL+A + +RY S + + G E + D++
Sbjct: 217 AIIKRLMTDVPFGVLLSGGLDSSLVASVTARYLADTKAAKQWGSKLHSFCVGLEGAPDLK 276
Query: 278 WARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGV 337
AR VA+ +G E ++ +D + IE +++ +E + + P++ +++ + GV
Sbjct: 277 AAREVADYIGTVHHEFQYTIQDGIDAIEDVIYHVETYDVTTIRAGTPMFLMSRKIKSLGV 336
Query: 338 KVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVETRY 391
K++LSG+G+DE+F GY + + P ++ L + + R +K G+E R
Sbjct: 337 KMVLSGEGSDEIFAGYLYFHKAPNKEELHQETCSKIKALHKYDCLRANKSTYAWGLEARV 396
Query: 392 PYLDLPFAILALNVPLELKI--RDGKR--KFILREI---AKSLGLPEWVAEREKK 439
P+LD F +A+ + E K+ RD R K++LR+ ++ LP+ + R+K+
Sbjct: 397 PFLDKKFIDVAMGIDPENKMIKRDEGRIEKWVLRKAFDDEENPYLPKHILYRQKE 451
>sp|Q43011|ASNS_ORYSA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|7438076|pir||T03602 probable
asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
- rice >gi|1432054|gb|AAB03991.1| (U55873) asparagine
synthetase [Oryza sativa] >gi|1902992|dbj|BAA18951.1|
(D83378) asparagine synthetase [Oryza sativa]
Length = 591
Score = 130 bits (324), Expect = 3e-29
Identities = 138/481 (28%), Positives = 220/481 (45%), Gaps = 81/481 (16%)
Query: 4 IAGGFNVSREDAIKIINLGK---HRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G +VS +II L + HRGPD WS + + L
Sbjct: 7 VLGVADVSLAKRSRIIELSRRLRHRGPD----WSGIHCYQ-------------DCYLAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP YNE +++ NGEIYNHE L+ L + F+T D EVI L E
Sbjct: 50 RLAIVDPTSGDQPLYNEDKSVVVTVNGEIYNHEELKANL--KSHKFQTASDCEVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G +A + F RD +G+ PLY
Sbjct: 108 -----------EYGEEFVDMLDGMFAFVLLDTRDKSFIAARDAIGICPLYMGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVL---PGEIVSISKHGITRVRGFNVFSIRTQGKDYFNGLQNLL 228
F+SE K L +D E + PG + S G+ R FS Y N L LL
Sbjct: 157 FSSEMKAL---SDDCERFISFPPGHLYSSKTGGLRRWYNPPWFSESIPSTPY-NPL--LL 210
Query: 229 IDSVKVRASKKV------GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTE 271
S + K++ GVL SGG+DSSL+A + SR+ + + + G +
Sbjct: 211 RQSFEKAIIKRLMTDVPFGVLLSGGLDSSLVASVVSRHLAEAKVAAQWGNKLHTFCIGLK 270
Query: 272 DSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKR 331
S D+ A+ VA+ LG E F+ ++ + +E +++ +E + + + P++ +++
Sbjct: 271 GSPDLRAAKEVADYLGTVHHELHFTVQEGIDALEEVIYHVETYDVTTIRASTPMFLMSRK 330
Query: 332 AREDGVKVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLN 385
+ GVK++LSG+G+DE+FGGY + + P + ++ L + R +K
Sbjct: 331 IKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAW 390
Query: 386 GVETRYPYLDLPFAILALNVPLELKI--RDGKR--KFILR---EIAKSLGLPEWVAEREK 438
GVE R P+LD F +A+++ E K+ RD R K++LR + + LP+ + R+K
Sbjct: 391 GVEARVPFLDKNFINVAMDIDPEWKMIKRDLGRIEKWVLRNAFDDEEKPYLPKHILYRQK 450
Query: 439 K 439
+
Sbjct: 451 E 451
>gi|6321563 asparagine synthetase; Asn2p [Saccharomyces cerevisiae]
>gi|1351986|sp|P49090|ASN2_YEAST ASPARAGINE SYNTHETASE
[GLUTAMINE-HYDROLYZING] 2 (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE 2) >gi|1084549|pir||S55982
asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
ASN2 - yeast (Saccharomyces cerevisiae)
>gi|642347|emb|CAA58159.1| (X83099) glutamic-dependent
asparagine synthase [Saccharomyces cerevisiae]
>gi|1323203|emb|CAA97135.1| (Z72909) ORF YGR124w
[Saccharomyces cerevisiae]
Length = 572
Score = 130 bits (323), Expect = 4e-29
Identities = 138/491 (28%), Positives = 227/491 (46%), Gaps = 97/491 (19%)
Query: 15 AIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLAMTGSKSYTQPF 74
A+++ +HRGPD WS ++ S F + RLA+ G S QP
Sbjct: 20 ALQLSKKIRHRGPD----WSGNAVMNSTIF-------------VHERLAIVGLDSGAQPI 62
Query: 75 YN---EIILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAI 131
+ E +L NGEIYNH LR+ + F+T D E I+ L YL D
Sbjct: 63 TSADGEYMLGVNGEIYNHIQLREMCSD--YKFQTFSDCEPIIPL--YLEHDID------- 111
Query: 132 KLSMKMLRGDYAVAFFFNGKFYLF--RDPLGVRPLYYSSRG------HFASEKKVLWGLG 183
+ K L G +A + + K + RDP+GV LY +FASE K L +
Sbjct: 112 --APKYLDGMFAFCLYDSKKDRIVAARDPIGVVTLYMGRSSQSPETVYFASELKCLTDVC 169
Query: 184 EDAEPVLPGEIVSISKHGITRV---RGFNVFSIRTQGKDYFNGLQNLLIDSVKVRASKKV 240
+ PG + ITR + I + DY + +++ L +V+ R +V
Sbjct: 170 DSIISFPPGHVYDSETDKITRYFTPDWLDEKRIPSTPVDY-HAIRHSLEKAVRKRLMAEV 228
Query: 241 --GVLFSGGVDSSLIALIASRYSDVI-----------------------LYTS------- 268
GVL SGG+DSSLIA IA+R ++ L+TS
Sbjct: 229 PYGVLLSGGLDSSLIAAIAARETEKANADANEDNNVDEKQLAGIDDQGHLHTSGWSRLHS 288
Query: 269 ---GTEDSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPL 325
G ++ D++ AR+VA+ +G E F+ ++ + ++ +++ +E + + + P+
Sbjct: 289 FAIGLPNAPDLQAARKVAKFIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIRASTPM 348
Query: 326 YFSTKRAREDGVKVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDD 379
+ +++ + GVK++LSG+G+DE+FGGY + Q P ++ V+ L + R +
Sbjct: 349 FLLSRKIKAQGVKMVLSGEGSDEIFGGYLYFAQAPSAAEFHTESVQRVKNLHLADCLRAN 408
Query: 380 KVAMLNGVETRYPYLDLPFAILALNV-PLE--LKIRDGK-RKFILREIAKSLG------- 428
K M G+E R P+LD F L +N+ P E +K ++G+ K+ILR+ +
Sbjct: 409 KSTMAWGLEARVPFLDKDFLQLCMNIDPNEKMIKPKEGRIEKYILRKAFDTTDEPDVKPY 468
Query: 429 LPEWVAEREKK 439
LPE + R+K+
Sbjct: 469 LPEEILWRQKE 479
>sp|P19891|ASNS_CRIGR ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|68657|pir||AJHYNC asparagine
synthase (glutamine-hydrolyzing) (EC 6.3.5.4) - Chinese
hamster >gi|191064|gb|AAA36977.1| (M27838) asparagine
synthetase [Cricetulus longicaudatus]
Length = 561
Score = 129 bits (322), Expect = 5e-29
Identities = 134/481 (27%), Positives = 224/481 (45%), Gaps = 83/481 (17%)
Query: 24 HRGPDSFGFWSERQMLKSDNFSDVSEIEGG--EIALIQCRLAMTGSKSYTQPFYNEIILV 81
HRGPD+F F +N + + G +A++ M + P+ + L
Sbjct: 26 HRGPDAFRF---------ENVNGYTNCCFGFHRLAVVDPLFGMQPIRVKKYPY---LWLC 73
Query: 82 HNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGD 141
+NGEIYNH++L++ ++T+VD E+IL L + + I+ ++ ML G
Sbjct: 74 YNGEIYNHKALQQRF---EFEYQTNVDGEIILHLYD----------KGGIEQTICMLDGV 120
Query: 142 YAVAFF--FNGKFYLFRDPLGVRPLY--YSSRGHFA--SEKKVLWGLGEDA------EPV 189
+A N K +L RD GVRPL+ + G A SE K L L EP
Sbjct: 121 FAFILLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVSLKHSTTPFLKVEPF 180
Query: 190 LPG--EIVSISKHGIT---------RVRGFNVFSIRTQGKDYFNGLQ--------NLLID 230
LPG E++ + +G R + ++ + F G + +L D
Sbjct: 181 LPGHYEVLDLKPNGKVASVEMVKYHHCRDEPLHALYDSVEKLFQGFELETVKSNLRILFD 240
Query: 231 SV---KVRASKKVGVLFSGGVDSSLIALI-------ASRYSDVILYTSGTEDSKDVEWAR 280
S ++ +++G L SGG+DSSL+A A + + G EDS D+ AR
Sbjct: 241 SAVRKRLMTDRRIGCLLSGGLDSSLVAASLLKQLKEAQVQYPLQTFAIGMEDSPDLLAAR 300
Query: 281 RVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTK--RAREDGVK 338
+VA +G + E LF+ E+ ++ ++F++E + + ++ +Y +K R D V
Sbjct: 301 KVANYIGSEHHEVLFNSEEGIQALDEVIFSLETYDITTVRASVGMYLISKYIRKNTDSV- 359
Query: 339 VLLSGQGADELFGGYAKYLQNPGLMLKDVEELGER--------NLARDDKVAMLNGVETR 390
V+ SG+G+DEL GY + + P + EE ER ++ R D+ +G+E R
Sbjct: 360 VIFSGEGSDELTQGYIYFHKAPS--PEKAEEESERLLKELYLFDVLRADRTTAAHGLELR 417
Query: 391 YPYLDLPFAILALNVPLELKI-RDGKRKFILREIAKSLG-LPEWVAEREKKAAQYGSNAQ 448
P+LD F+ L++P E++I ++G K +LRE + LP+ + R K+A G +
Sbjct: 418 VPFLDHRFSSYYLSLPPEMRIPKNGIEKHLLRETFEDSNLLPKEILWRPKEAFSDGITSV 477
Query: 449 K 449
K
Sbjct: 478 K 478
>emb|CAA96526.1| (Z72354) asparagine synthetase [Vicia faba]
Length = 586
Score = 129 bits (321), Expect = 7e-29
Identities = 135/477 (28%), Positives = 214/477 (44%), Gaps = 72/477 (15%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++I+ L KHRGPD G DN+ L
Sbjct: 7 VLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQH-----GDNY------------LAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE II+ NGEIYNHE LRK L F T D +VI L E
Sbjct: 50 RLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEELRKQLPNH--TFRTQCDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + +L G ++ + F + RD +GV LY
Sbjct: 108 -----------EYGENFVDLLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRG---FNVFSIRTQGKDYFNGLQNLL 228
ASE K L E E PG + S + R FN I + D L+
Sbjct: 157 IASELKGLNDECEHFEVFPPGHLYSSKEREFRRWYNPPWFNEAIIPSTPYDPLV-LRTAF 215
Query: 229 IDSVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKD 275
+V R V GVL SGG+DSSL+A + +RY + + + G + + D
Sbjct: 216 EKAVIKRLMTDVPFGVLLSGGLDSSLVASVTARYLADTKAAKQWGAKLHSFCVGLKGAPD 275
Query: 276 VEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRARED 335
++ + VA+ LG E F+ +D + IE +++ E + + A P++ +++ +
Sbjct: 276 LKAGKEVADFLGTVHHEFQFTIQDGIDAIEDVIYHTETYDVTTIRAATPMFLMSRKIKSS 335
Query: 336 GVKVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVET 389
GVK+L+SG+G+DE+FGGY + + P + ++ L + R +K G+E
Sbjct: 336 GVKMLISGEGSDEIFGGYLYFHKAPNKEEFHQETCRKIKALHRYDCLRANKSTYAWGLEA 395
Query: 390 RYPYLDLPFAILALNVPLELKI---RDGK-RKFILREI---AKSLGLPEWVAEREKK 439
R P+LD F A+++ E K+ +G+ K+ILR+ ++ LP+ + R+K+
Sbjct: 396 RVPFLDKDFIKAAMDIDPEFKMIKHDEGRIEKWILRKAFDDEENPYLPKHILYRQKE 452
>pir||T39308 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) SPBC119.10
[similarity] - fission yeast (Schizosaccharomyces pombe)
>gi|2959371|emb|CAA17925.1| (AL022117) asparagine
synthetase [Schizosaccharomyces pombe]
Length = 557
Score = 129 bits (321), Expect = 7e-29
Identities = 132/463 (28%), Positives = 208/463 (44%), Gaps = 74/463 (15%)
Query: 4 IAGGFNVSREDAIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLA 63
+A + A+ + +HRGPD WS + + + L RLA
Sbjct: 10 VAEDIEAFKPKALHLSKQLRHRGPD----WSGKAIRN-------------QTILCHERLA 52
Query: 64 MTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLV 120
+ G +S QP ++ ++L NGEIYNH LR+ L + F+T D EVIL L
Sbjct: 53 IVGVESGAQPLVSDDGKLVLTVNGEIYNHLKLRENL-KGNYKFKTYSDCEVILYLYR--- 108
Query: 121 FDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLF--RDPLGVRPLY--YSS----RGHF 172
E ML G ++ + K + RDP+G+ LY +SS +F
Sbjct: 109 --------EHGPACANMLDGMFSWVLYDQDKDKVVAARDPIGITTLYQGFSSDSPDTAYF 160
Query: 173 ASEKKVLWGLGEDAEPVLPGEIVSISKHGITRV---RGFNVFSIRTQGKDYFNGLQNLLI 229
ASE K L + + PG R ++ I + DY L+ L
Sbjct: 161 ASELKALHPVCDKIIAFPPGHYYDSETKQTVRYFKPSWWDENKIPSNPVDY-KLLRETLE 219
Query: 230 DSVKVRASKKV--GVLFSGGVDSSLIALIASRYSDVIL--------------------YT 267
SV+ R +V GVL SGG+DSSLIA IA+R ++ + +
Sbjct: 220 ASVRKRLMAEVPYGVLLSGGLDSSLIASIAARETEKLANSTSQSEEARTITAWPKLHSFA 279
Query: 268 SGTEDSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYF 327
G S D+ AR+VA+ L E F+ ++ + + +++ +E + + + P+Y
Sbjct: 280 IGLPGSPDLLAARKVADFLHTFHHEHTFTIDEGLDALRDVIYHLETYDVTTIRASTPMYL 339
Query: 328 STKRAREDGVKVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKV 381
+++ + GVK++LSG+G+DE+FGGY + P ++ V+ L + R +K
Sbjct: 340 LSRKIKAQGVKMVLSGEGSDEIFGGYLYFGNAPSREAFHSECVRRVKNLHLSDCLRANKS 399
Query: 382 AMLNGVETRYPYLDLPFAILALNVPLELK--IRDGKRKFILRE 422
M G+E R P+LD F +ALN+ E K I K K+ILR+
Sbjct: 400 TMAWGLEARVPFLDKDFLEVALNIDPEEKMYINGRKEKYILRK 442
>pir||AJHUN2 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) (version
2) - human >gi|339987|gb|AAA36781.1| (M15798) ts11 cell
cycle control protein [Homo sapiens]
Length = 540
Score = 129 bits (320), Expect = 9e-29
Identities = 135/487 (27%), Positives = 225/487 (45%), Gaps = 95/487 (19%)
Query: 24 HRGPDSFGFWSERQMLKSDNFSDVSEIEGG--EIALIQCRLAMTGSKSYTQPFYNEIILV 81
HRGPD+F F +N + + G +A++ M + P+ + L
Sbjct: 5 HRGPDAFRF---------ENVNGYTNCCFGFHRLAVVDPLFGMQPIRVKKYPY---LWLC 52
Query: 82 HNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGD 141
+NGEIYNH+ ++++ ++T VD E+IL L + + I+ ++ ML G
Sbjct: 53 YNGEIYNHKKMQQHF---EFEYQTKVDGEIILHLYD----------KGGIEQTICMLDGV 99
Query: 142 YAVAFF--FNGKFYLFRDPLGVRPLY--YSSRGHFA--SEKKVLWGLGEDA------EPV 189
+A N K +L RD GVRPL+ + G A SE K L L A EP
Sbjct: 100 FAFVLLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVTLKHSATPFLKVEPF 159
Query: 190 LPG--EIVSISKHG----ITRVR---------------------GFNVFSIRTQGKDYFN 222
LPG E++ + +G + V+ GF + +++ N
Sbjct: 160 LPGHYEVLDLKPNGKVASVEMVKYHHCRDVPLHALYDNVEKLFPGFEIETVK-------N 212
Query: 223 GLQNLLIDSVKVR--ASKKVGVLFSGGVDSSLIALI-------ASRYSDVILYTSGTEDS 273
L+ L ++VK R +++G L SGG+DSSL+A A + + G EDS
Sbjct: 213 NLRILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKEAQVQYPLQTFAIGMEDS 272
Query: 274 KDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKR-A 332
D+ AR+VA+ +G + E LF+ E+ ++ ++F++E + I +Y +K
Sbjct: 273 PDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLELMTLQQFVLRIGMYLISKYIG 332
Query: 333 REDGVKVLLSGQGADELFGGYAKYLQNPGLMLKDVEELGER--------NLARDDKVAML 384
R ++ SG+G+DEL GY + + P + EE ER ++ R D+
Sbjct: 333 RTQIAWLIFSGEGSDELTQGYIYFHKAPS--PEKAEEESERLLRELYLFDVLRADRTTAA 390
Query: 385 NGVETRYPYLDLPFAILALNVPLELKI-RDGKRKFILREIAKSLGL-PEWVAEREKKAAQ 442
+G+E R P+LD F+ L++P E++I ++G K +LRE + L P+ + R K+A
Sbjct: 391 HGLELRVPFLDHRFSSYYLSLPPEMRIPKNGIEKHLLRETFEDSNLIPKEILWRPKEAFS 450
Query: 443 YGSNAQK 449
G + K
Sbjct: 451 DGITSVK 457
>sp|P19251|ASN1_PEA ASPARAGINE SYNTHETASE, NODULE [GLUTAMINE-HYDROLYZING]
(GLUTAMINE-DEPENDENT ASPARAGINE SYNTHETASE)
>gi|68661|pir||AJPMN1 asparagine synthase
(glutamine-hydrolyzing) (EC 6.3.5.4) 1 - garden pea
>gi|20650|emb|CAA36429.1| (X52179) asparagine synthase
(glutamine-hydrolysing) [Pisum sativum]
Length = 586
Score = 129 bits (320), Expect = 9e-29
Identities = 136/477 (28%), Positives = 215/477 (44%), Gaps = 72/477 (15%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++I+ L KHRGPD G DN+ L
Sbjct: 7 VLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQH-----GDNY------------LAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE II+ NGEIYNHE LRK L F T D +VI L E
Sbjct: 50 RLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEELRKQLPNH--KFFTQCDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G ++ + F + RD +GV LY
Sbjct: 108 -----------EHGENFVDMLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRG---FNVFSIRTQGKDYFNGLQNLL 228
ASE K L E E PG + S + R FN I + D L+N
Sbjct: 157 IASELKGLNDECEHFEVFPPGHLYSSKEREFRRWYNPPWFNEAIIPSTPYDPLV-LRNAF 215
Query: 229 IDSVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKD 275
+V R V GVL SGG+DSSL+A + +RY + + + G + + D
Sbjct: 216 EKAVIKRLMTDVPFGVLLSGGLDSSLVASVTARYLAGTKAAKQWGAKLPSFCVGLKGAPD 275
Query: 276 VEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRARED 335
++ + VA+ LG E F+ +D + IE +++ E + + A P++ +++ +
Sbjct: 276 LKAGKEVADFLGTVHHEFEFTIQDGIDAIEDVIYHTETYDVTTIRAATPMFLMSRKIKSS 335
Query: 336 GVKVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVET 389
GVK ++SG+G+DE+FGGY + + P + ++ L + R +K G+E
Sbjct: 336 GVKWVISGEGSDEIFGGYLYFHKAPNREEFHQETCRKIKALHRYDCLRANKSTYAWGLEA 395
Query: 390 RYPYLDLPFAILALNVPLELKI---RDGK-RKFILREI---AKSLGLPEWVAEREKK 439
R P+LD F +A+++ E K+ +G+ K+ILR+ ++ LP+ + R+K+
Sbjct: 396 RVPFLDKDFIKVAMDIDPEFKMIKHDEGRIEKWILRKAFDDEENPYLPKHILYRQKE 452
>sp|Q61024|ASNS_MOUSE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|1147605|gb|AAA85125.1|
(U38940) asparagine synthetase [Mus musculus]
Length = 561
Score = 128 bits (318), Expect = 2e-28
Identities = 135/488 (27%), Positives = 229/488 (46%), Gaps = 97/488 (19%)
Query: 24 HRGPDSFGFWSERQMLKSDNFSDVSEIEGG--EIALIQCRLAMTGSKSYTQPFYNEIILV 81
HRGPD+F F +N + + G +A++ M + P+ + L
Sbjct: 26 HRGPDAFRF---------ENVNGYTNCCFGFHRLAVVDPLFGMQPIRVRKYPY---LWLC 73
Query: 82 HNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGD 141
+NGEIYNH++L++ ++T+VD E+IL L + + I+ ++ ML G
Sbjct: 74 YNGEIYNHKALQQRF---EFEYQTNVDGEIILHLYD----------KGGIEKTICMLDGV 120
Query: 142 YAVAFF--FNGKFYLFRDPLGVRPLY--YSSRGHFA--SEKKVLWGLGED------AEPV 189
+A N K +L RD GVRPL+ + G A SE K L L EP
Sbjct: 121 FAFILLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVSLKHSTTPFLKVEPF 180
Query: 190 LPG--EIVSISKHG----ITRVR---------------------GFNVFSIRTQGKDYFN 222
LPG E++ + +G + V+ GF++ +++ N
Sbjct: 181 LPGHYEVLDLKPNGKVASVEMVKYHHCTDEPLHAIYDSVEKLFPGFDLETVK-------N 233
Query: 223 GLQNLLIDSVKVR--ASKKVGVLFSGGVDSSLIALI-------ASRYSDVILYTSGTEDS 273
L+ L +++K R +++G L SGG+DSSL+A A + + G EDS
Sbjct: 234 NLRILFDNAIKKRLMTDRRIGCLLSGGLDSSLVAASLLKQLKEAQVQYPLQTFAIGMEDS 293
Query: 274 KDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTK--R 331
D+ AR+VA +G + E LF+ E+ ++ ++F++E + + ++ +Y +K R
Sbjct: 294 PDLLAARKVANYIGSEHHEVLFNSEEGIQALDEVIFSLETYDITTVRASVGMYLISKYIR 353
Query: 332 AREDGVKVLLSGQGADELFGGYAKYLQNPGLMLKDVEELGER--------NLARDDKVAM 383
D V V+ SG+G+DEL GY + + P + EE ER ++ R D+
Sbjct: 354 KNTDSV-VIFSGEGSDELTQGYIYFHKAPS--PEKAEEESERLLKELYLFDVLRADRTTA 410
Query: 384 LNGVETRYPYLDLPFAILALNVPLELKI-RDGKRKFILREIAKSLG-LPEWVAEREKKAA 441
+G+E R P+LD F+ L++P +++I ++G K +LRE + LP+ + R K+A
Sbjct: 411 AHGLELRVPFLDHRFSSYYLSLPPDMRIPKNGIEKHLLRETFEDCNLLPKEILWRPKEAF 470
Query: 442 QYGSNAQK 449
G + K
Sbjct: 471 SDGITSVK 478
>sp|P49091|ASNS_BRAOL ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|1084354|pir||S52387
asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
- wild cabbage >gi|669057|emb|CAA59138.1| (X84448)
asparagine synthase (glutamine-hydrolysing) [Brassica
oleracea]
Length = 586
Score = 128 bits (318), Expect = 2e-28
Identities = 134/477 (28%), Positives = 221/477 (46%), Gaps = 72/477 (15%)
Query: 4 IAGGFNVSREDAIKIINLGK---HRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++++ L + HRGPD G + + G L
Sbjct: 7 VLGCSDDSQAKRVRVLELSRRLRHRGPDWSGIY-----------------QNGFNYLAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE I++ NGEIYNHE LRK L + F T D +VI L E
Sbjct: 50 RLAIIDPDSGDQPLFNEDKSIVVTVNGEIYNHEELRKGL--KNHKFHTGSDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLY--YSSRGHF- 172
E + + ML G ++ + F + RD +GV LY + G
Sbjct: 108 -----------EHGENFVDMLDGIFSFVLLDTRDNSFMVARDAVGVTSLYIGWGLDGSLW 156
Query: 173 -ASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNV--FSIRTQGKDYFN-GLQNLL 228
+SE K L E E PG + S SK G + +N F+ Y +++
Sbjct: 157 VSSEMKGLHEDCEHFEAFPPGHLYS-SKSGGGFKQWYNPPWFNESVPSTPYEPLAIRSAF 215
Query: 229 IDSVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKD 275
D+V R V GVL SGG+DSSL+A I +R+ + + G E S D
Sbjct: 216 EDAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLAGTKAAKRWGPQLHSFCVGLEGSPD 275
Query: 276 VEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRARED 335
++ + VAE LG E F+ +D + IE +++ +E + + + P++ +++ +
Sbjct: 276 LKAGKEVAEYLGTVHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSL 335
Query: 336 GVKVLLSGQGADELFGGYAKYLQNPGLM------LKDVEELGERNLARDDKVAMLNGVET 389
GVK++LSG+G+DE+FGGY + + P + ++ L + + R +K G+E
Sbjct: 336 GVKMVLSGEGSDEIFGGYLYFHKAPNKQEFHQETCRKIKALHKYDCLRANKATSAFGLEA 395
Query: 390 RYPYLDLPFAILALNVPLE---LKIRDGK-RKFILR---EIAKSLGLPEWVAEREKK 439
R P+LD F A+++ E +K +G+ K++LR + + LP+ + R+K+
Sbjct: 396 RVPFLDKEFINTAMSLDPESKMIKPEEGRIEKWVLRRAFDDEERPYLPKHILYRQKE 452
>dbj|BAB17726.1| (AB050900) asparagine synthetase [Raphanus sativus]
Length = 585
Score = 128 bits (318), Expect = 2e-28
Identities = 134/477 (28%), Positives = 220/477 (46%), Gaps = 72/477 (15%)
Query: 4 IAGGFNVSREDAIKIINLGK---HRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++++ L + HRGPD G + + G L
Sbjct: 7 VLGCSDDSQAKRVRVLELSRRLRHRGPDWSGIY-----------------QNGGNYLAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE I++ NGEIYNHE LRK L + F T D +VI L E
Sbjct: 50 RLAIIDPASGDQPLFNEDKSIVVTVNGEIYNHEELRKGL--KNHKFHTGSDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLY--YSSRGHF- 172
E + + ML G ++ + F + RD +GV LY + G
Sbjct: 108 -----------EHGENFVDMLDGIFSFVLLDTRDNSFMVARDAVGVTSLYIGWGLDGSLW 156
Query: 173 -ASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNV--FSIRTQGKDYFN-GLQNLL 228
+SE K L E E PG + S SK G + +N F+ Y ++
Sbjct: 157 VSSEMKGLHEDCEHFEAFPPGHLYS-SKSGGGFKQWYNPPWFNESVPSTPYEPLAIRRAF 215
Query: 229 IDSVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKD 275
D+V R V GVL SGG+DSSL+A I +R+ + + G E S D
Sbjct: 216 EDAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLAGTKAAKRWGPQLHSFCVGLEGSPD 275
Query: 276 VEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRARED 335
++ + VAE LG E F+ +D + IE +++ +E + + + P++ +++ +
Sbjct: 276 LKAGKEVAEYLGTVHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSL 335
Query: 336 GVKVLLSGQGADELFGGYAKYLQNPGLM------LKDVEELGERNLARDDKVAMLNGVET 389
GVK++LSG+G+DE+FGGY + + P + ++ L + + R +K G+E
Sbjct: 336 GVKMVLSGEGSDEIFGGYLYFHKAPNKQEFHQETCRKIKALHKYDCLRANKATSAFGLEA 395
Query: 390 RYPYLDLPFAILALNVPLE---LKIRDGK-RKFILR---EIAKSLGLPEWVAEREKK 439
R P+LD F A+++ E +K +G+ K++LR + + LP+ + R+K+
Sbjct: 396 RVPFLDKEFINTAMSLDPESKMIKPEEGRIEKWVLRRAFDDEERPYLPKHILYRQKE 452
>sp|P49094|ASNS_MAIZE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|7438077|pir||T02978
asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
- maize >gi|984262|emb|CAA58052.1| (X82849) asparragine
synthetase [Zea mays]
Length = 586
Score = 127 bits (316), Expect = 3e-28
Identities = 125/441 (28%), Positives = 197/441 (44%), Gaps = 70/441 (15%)
Query: 4 IAGGFNVSREDAIKIINLGK---HRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G VS +II L + HRGPD G + L
Sbjct: 7 VLGVVEVSLAKRSRIIELSRRLRHRGPDWSGLHCHE-----------------DCYLAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP YNE +++ NGEIYNHE L+ L + F+T D EVI L E
Sbjct: 50 RLAIIDPTSGDQPLYNEDKTVVVTVNGEIYNHEELKAKL--KTHEFQTGSDCEVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G ++ + F RD +G+ PLY
Sbjct: 108 -----------EYGEEFVDMLDGMFSFVLLDTRDKSFIAARDAIGICPLYMGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVL---PGEIVSISKHGITRVRGFNVFSIRTQGKDYFNGL--QN 226
F+SE K L +D E + PG + S G+ R FS Y N L +
Sbjct: 157 FSSEMKAL---SDDCERFITFPPGHLYSSKTGGLRRWYNPPWFSETVPSTPY-NALFLRE 212
Query: 227 LLIDSVKVRASKKV--GVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDS 273
+ +V R V GVL SGG+DSSL+A +ASR+ + + + G + S
Sbjct: 213 MFEKAVIKRLMTDVPFGVLLSGGLDSSLVASVASRHLNETKVDRQWGNKLHTFCIGLKGS 272
Query: 274 KDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAR 333
D++ AR VA+ L E F+ ++ + +E +++ IE + + + P++ +++ +
Sbjct: 273 PDLKAAREVADYLSTVHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIK 332
Query: 334 EDGVKVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGV 387
GVK+++SG+G+DE+FGGY + + P + ++ L + R +K GV
Sbjct: 333 SLGVKMVISGEGSDEIFGGYLYFHKAPNKKEFLEETCRKIKALHLYDCLRANKATSAWGV 392
Query: 388 ETRYPYLDLPFAILALNVPLE 408
E R P+LD F +A+++ E
Sbjct: 393 EARVPFLDKSFISVAMDIDPE 413
>gb|AAC49614.1| (U77679) asparagine synthetase 1 [Glycine max]
Length = 579
Score = 127 bits (315), Expect = 4e-28
Identities = 121/442 (27%), Positives = 195/442 (43%), Gaps = 66/442 (14%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++++ L KHRGPD WS DN+ L
Sbjct: 7 VLGCSDSSQAKRVRVLELSRRLKHRGPD----WSGLHQY-GDNY------------LAHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP +NE +++ NGEIYNHE LRK L F T D +VI L E
Sbjct: 50 RLAIVDPASGDQPLFNEDKTVVVTVNGEIYNHEELRKQLPNH--TFRTGSDCDVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRG--DYAVAFFFNGKFYLFRDPLGVRPLYYS----SRGH 171
E + M ML G + + + F + RD +GV LY
Sbjct: 108 -----------EHGENFMDMLDGISSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYF-----NGLQN 226
+SE K L E E PG + S + R S+ Y + +
Sbjct: 157 ISSELKGLNDDCEHFESFPPGHLYSSKERAFRRWYNPPWLSLAIPSAPYDPLALRHAFEK 216
Query: 227 LLIDSVKVRASKKVGVLFSGGVDSSLIALIASRY-----------SDVILYTSGTEDSKD 275
L I ++ GVL SGG+DSSL+A + +RY + + + G E + D
Sbjct: 217 LWIK--RLMTDVPFGVLLSGGLDSSLVAAVTARYLAGTKAAKQWGTKLHSFCVGLEGAPD 274
Query: 276 VEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRARED 335
++ + VAE +G E ++ +D + IE +++ IE + + +IP++ +++ +
Sbjct: 275 LKATKEVAEYIGTVHHEFHYTVQDGIDAIEDVIYHIETYDVTTIRASIPMFLMSRKIKSL 334
Query: 336 GVKVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARDDKVAMLNGVET 389
GVK ++SG+G+D FGGY + + P + + L + +R +K + G+E
Sbjct: 335 GVKWVISGEGSDVFFGGYLYFHKAPNKEEFHQETCRTIIVLHRYDCSRANKSTFVWGLEA 394
Query: 390 RYPYLDLPFAILALNVPLELKI 411
R P+LD F +A+N+ E K+
Sbjct: 395 RVPFLDKEFIRVAMNIDPECKM 416
>pir||D82846 asparagine synthase B XF0118 [imported] - Xylella fastidiosa
(strain 9a5c) >gi|9104900|gb|AAF82931.1|AE003865_8
(AE003865) asparagine synthase B [Xylella fastidiosa]
Length = 563
Score = 125 bits (312), Expect = 8e-28
Identities = 127/463 (27%), Positives = 200/463 (42%), Gaps = 86/463 (18%)
Query: 1 MCLIAGGFNVS--------REDAIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEG 52
MC I G FN+ R A++ +HRGPD G + DV I
Sbjct: 1 MCSIFGIFNLQPSDNLQRLRHQALECSQRQRHRGPDWSGV-----------YVDVGAI-- 47
Query: 53 GEIALIQCRLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDS 109
L+ RLA+ QP +E + L NGEIYNH L K +++ F+T D
Sbjct: 48 ----LVHERLAIVDPAGGAQPLLSEDGTLALAVNGEIYNHAVL-KGTLQQPYVFQTHSDC 102
Query: 110 EVILRLLEYLVFDRKLKVEEAIKLSMKMLRGDYAVAFF--FNGKFYLFRDPLGVRPLYYS 167
EVI L EE + L G +A A + G+ + RDP+GV PLY+
Sbjct: 103 EVINALYR----------EETPTSFLNRLNGIFAFALWDKTTGRGLIARDPMGVVPLYWG 152
Query: 168 ----SRGHFASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFNG 223
R ASE K L D PG H G V K+Y +
Sbjct: 153 HDQDGRLRVASEMKALIEHCPDVAQFPPG-------HWYDTTTGTLVKYYERSWKNY-SA 204
Query: 224 LQNLLIDSVKVRASKK------------VGVLFSGGVDSSLIALIASRYSDVILYTS--- 268
++ + + ++R + + GVL SGG+DSSL+A +A+RY+ + T+
Sbjct: 205 VEGVQVSLQELREAFERAVHRQLMTDVPYGVLLSGGLDSSLVAAVAARYARHRIETNDQS 264
Query: 269 ------------GTEDSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNP 316
G +DS D+ A AE L ++ ++ + + ++ IE +
Sbjct: 265 EAWWPRLHSFAIGLKDSPDLSAANVAAEALNTVHHGFEYTLQEGLDVLPEVIRHIETYDV 324
Query: 317 MNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGYAKYLQNPGL------MLKDVEEL 370
+ + P++ +R + GVK++LSG+G+DE+FGGY + + P +++ + L
Sbjct: 325 TTIRASTPMFLLARRIKAMGVKMVLSGEGSDEIFGGYLYFHKAPNAREFHEELVRKLNAL 384
Query: 371 GERNLARDDKVAMLNGVETRYPYLDLPFAILALNVPLELKIRD 413
+ R +K M GVE R P+LD F +A+ + + K+ D
Sbjct: 385 YYYDCLRANKAMMAWGVEPRVPFLDREFLDVAMRMDAQHKMVD 427
>gi|6325403 asparagine synthetase; Asn1p [Saccharomyces cerevisiae]
>gi|1351984|sp|P49089|ASN1_YEAST ASPARAGINE SYNTHETASE
[GLUTAMINE-HYDROLYZING] 1 (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE 1) >gi|1077018|pir||S52694
asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
ASN1 - yeast (Saccharomyces cerevisiae)
>gi|747902|emb|CAA88594.1| (Z48675) asparagine
synthetase [Saccharomyces cerevisiae]
>gi|1066479|gb|AAB68284.1| (U40829) Similar to
Asparagine synthetase B from E. coli (Swiss Prot.
accession number P22106) and other species
[Saccharomyces cerevisiae]
Length = 572
Score = 125 bits (310), Expect = 1e-27
Identities = 135/492 (27%), Positives = 221/492 (44%), Gaps = 98/492 (19%)
Query: 15 AIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLAMTGSKSYTQPF 74
A+++ +HRGPD WS + S F + RLA+ G +S QP
Sbjct: 20 ALQLSKRIRHRGPD----WSGNAIKNSTIF-------------VHERLAIVGVESGAQPI 62
Query: 75 YN---EIILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAI 131
+ E +L NGEIYNH LR+ + F T D E I+ + YL D
Sbjct: 63 TSSDGEYMLCVNGEIYNHIQLREECAD--YEFGTLSDCEPIIPM--YLKHDID------- 111
Query: 132 KLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYSSRG------HFASEKKVLWGLG 183
+ K L G +A + + RDP+G+ LY +FASE K L
Sbjct: 112 --APKYLDGMFAWTLYDAKQDRIVAARDPIGITTLYMGRSSASPKTVYFASELKCLTDDC 169
Query: 184 EDAEPVLPGEIVSISKHGITRV---RGFNVFSIRTQGKDYFNGLQNLLIDSVKVRASKKV 240
+ PG + ITR + I + DY +++ L +V+ R +V
Sbjct: 170 DTITAFPPGHVYDSKTDKITRYFTPDWLDEKRIPSTPIDYM-AIRHSLEKAVRKRLMAEV 228
Query: 241 --GVLFSGGVDSSLIALIASR-------------YSDVILYTSGTED------------- 272
GVL SGG+DSSLIA IA+R Y + +G +D
Sbjct: 229 PYGVLLSGGLDSSLIASIAARETAKATNDVEPSTYDSKARHLAGIDDDGKLHTAGWTSLH 288
Query: 273 --------SKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIP 324
+ D++ AR+VA+ +G E F+ ++ + ++ +++ +E + + + P
Sbjct: 289 SFAIGLPNAPDLQAARKVAKFIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIRASTP 348
Query: 325 LYFSTKRAREDGVKVLLSGQGADELFGGYAKYLQNPGL------MLKDVEELGERNLARD 378
++ +++ + GVK++LSG+G+DE+FGGY + Q P ++ V+ L + R
Sbjct: 349 MFLLSRKIKAQGVKMVLSGEGSDEIFGGYLYFAQAPSAAEFHTESVQRVKNLHLADCLRA 408
Query: 379 DKVAMLNGVETRYPYLDLPFAILALNV-PLE--LKIRDGK-RKFILREIAKSLG------ 428
+K M G+E R P+LD F L +N+ P E +K ++G+ K+ILR+ + G
Sbjct: 409 NKSTMAWGLEARVPFLDREFLQLCMNIDPNEKMIKPKEGRIEKYILRKAFDTTGEPDAKP 468
Query: 429 -LPEWVAEREKK 439
LPE + R+K+
Sbjct: 469 YLPEEILWRQKE 480
>sp|P17714|ASNS_MESAU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|68658|pir||AJHYNG asparagine
synthase (glutamine-hydrolyzing) (EC 6.3.5.4) - golden
hamster >gi|49614|emb|CAA36375.1| (X52130) asparagine
synthetase (AA 1-561) [Mesocricetus auratus]
Length = 561
Score = 123 bits (307), Expect = 3e-27
Identities = 132/481 (27%), Positives = 224/481 (46%), Gaps = 83/481 (17%)
Query: 24 HRGPDSFGFWSERQMLKSDNFSDVSEIEGG--EIALIQCRLAMTGSKSYTQPFYNEIILV 81
HRGPD+F F +N + + G +A++ M + P+ + L
Sbjct: 26 HRGPDAFRF---------ENVNGYTNCCFGFHRLAVVDPLFGMQPIRVKKYPY---LWLC 73
Query: 82 HNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGD 141
+NGEIYNH++L++ ++T+VD E+IL L + + I+ ++ ML G
Sbjct: 74 YNGEIYNHKALQQRF---EFEYQTNVDGEIILHLYD----------KGGIEQTICMLDGV 120
Query: 142 YAVAFF--FNGKFYLFRDPLGVRPLY--YSSRGHFA--SEKKVLWGLGEDA------EPV 189
+A N K +L RD GVRPL+ + G A SE K L L EP
Sbjct: 121 FAFILLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVSLKHSTTPFLKVEPF 180
Query: 190 LPG--EIVSISKHGIT---------RVRGFNVFSIRTQGKDYFNGLQ--------NLLID 230
LPG E++ + +G R + ++ + F G + +L D
Sbjct: 181 LPGHYEVLDLKPNGKVASVEMVKYHHCRDEPLHALYDSVEKLFPGFELETVKSNLRILFD 240
Query: 231 SV---KVRASKKVGVLFSGGVDSSLIA--LIASRYSDVILY-----TSGTEDSKDVEWAR 280
+ ++ +++ L SGG+DSSL+A L+ + Y G EDS D+ AR
Sbjct: 241 NAVRKRLMTDRRIVCLLSGGLDSSLVASSLLKQLKEAQVQYPLQTFAIGMEDSPDLLAAR 300
Query: 281 RVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTK--RAREDGVK 338
+VA +G + E LF+ E+ ++ ++F++E + + ++ +Y +K R D V
Sbjct: 301 KVANYIGSEHHEVLFNSEEGIQALDEVIFSLETYDITTVRASVGMYLISKYIRKNTDSV- 359
Query: 339 VLLSGQGADELFGGYAKYLQNPGLMLKDVEELGER--------NLARDDKVAMLNGVETR 390
V+ SG+G+DEL GY + + P + EE ER ++ R D+ +G+E R
Sbjct: 360 VIFSGEGSDELTQGYIYFHKAPS--PEKAEEESERLLKELYLFDVLRADRTTAAHGLELR 417
Query: 391 YPYLDLPFAILALNVPLELKI-RDGKRKFILREIAKSLG-LPEWVAEREKKAAQYGSNAQ 448
P+LD F+ L++P E+++ ++G K +LRE + LP+ + R K+A G +
Sbjct: 418 VPFLDHRFSSYYLSLPPEMRVPKNGIEKHLLRETFEDSNLLPKEILWRPKEAFSDGITSV 477
Query: 449 K 449
K
Sbjct: 478 K 478
>gb|AAG19311.1| (AE005026) asparagine synthetase; AsnA [Halobacterium sp. NRC-1]
Length = 360
Score = 120 bits (299), Expect = 3e-26
Identities = 92/241 (38%), Positives = 119/241 (49%), Gaps = 19/241 (7%)
Query: 234 VRASKKVGVLFSGGVDSSLIALIASRYSDVILYTSGTEDSKDVEWARRVAEELGLK--LR 291
V A + V FSGGVDS+L A D LY G DS D+ AR A +G LR
Sbjct: 109 VPAGDDLAVAFSGGVDSALAAAAL----DAPLYVVGYADSSDIAAARESAAAMGRADDLR 164
Query: 292 ESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFG 351
+ DIE + + AI N M + IA+PL+ RA DG L+ GQGADELFG
Sbjct: 165 VHEVTLADIEAAVPTVARAIGRTNAMAVQIALPLFLVAARAAADGYSRLVVGQGADELFG 224
Query: 352 GYAKYLQNP-----------GLMLKDVEELGERNLARDDKVAMLNGVETRYPYLDLPFAI 400
GYAK + P G + V+ L L RD + GVE P L
Sbjct: 225 GYAKVAKAPTDPRTDADTVRGARRETVQSL-PAQLERDLRALRAAGVEPVTPLLHDDVVR 283
Query: 401 LALNVPLELKIRDGKRKFILREIAKSLGLPEWVAEREKKAAQYGSNAQKILEKIAKSRGV 460
AL +P E + RK LRE A+S LP+ VA+R+KKA QYGS + ++++A+ G
Sbjct: 284 AALALPGEWLVSGDTRKRALREAARS-WLPDAVADRDKKAVQYGSLVAREVDRLARQAGF 342
Query: 461 K 461
K
Sbjct: 343 K 343
>emb|CAB58327.1| (AL121855) putative amidotransferase [Streptomyces coelicolor
A3(2)]
Length = 644
Score = 119 bits (296), Expect = 6e-26
Identities = 121/411 (29%), Positives = 186/411 (44%), Gaps = 78/411 (18%)
Query: 1 MCLIAGGFNVSREDAI--KIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALI 58
MC IAG ++ A+ ++ ++ HRGPD G +S + E+ G L
Sbjct: 1 MCGIAGTYHWPDGKAVTDRLTDVLAHRGPDGAGRYSHP--------AGEGEVHLGHRRLA 52
Query: 59 QCRLAMTGSKSYTQPFYNE-IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
L+ TG+ QP +E + L +NGE+YN LR L GV F D+EV+L
Sbjct: 53 VVDLSTTGA----QPMVSEGLALSYNGELYNAPELRTELESAGVRFRGTSDTEVVLEAWR 108
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFFN--GKFYLFRDPLGVRPLYYSSRGH---F 172
D + LRG +A A F G+ L RD LG++PL+ RG F
Sbjct: 109 RWGTD-----------CLPQLRGMFAFAVFDERTGELVLARDQLGIKPLFLLRRGAGLVF 157
Query: 173 ASEKKVL----------------------WGLGE-----DAEPVLPGEIVSISKHG-ITR 204
ASE K L W +AE + PG + G + R
Sbjct: 158 ASELKALATATGGKLEVDHAALVASLLYYWVPDSRCALREAEKLPPGSWLRCRPDGRVER 217
Query: 205 VRGFNVFSIRTQGKDYFNGLQNLLIDSVKVRASKK-------VGVLFSGGVDSSLIALIA 257
R +N+ + +G++ + + +V ++++ V SGG+DSS + +A
Sbjct: 218 GRFWNLRDVAAEGRERARSGERPDLAAVVEESTRRHLLSDVPVATFLSGGLDSSYLTALA 277
Query: 258 SR-YSDVILYTSG--TEDSK------DVEWARRVAEELGLKLRESLFSREDIENEIERIM 308
+R + YT G ED++ D+ +AR+VAE G+ L E + ++ + + R+
Sbjct: 278 ARDRPGISAYTIGFRAEDARFEAMPDDLRYARQVAERFGVDLHEIEIA-PNVLDLLPRMT 336
Query: 309 FAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGYAKYLQN 359
+ ++EP + AI + ARE GVKV+LSG GADELF GY K+L N
Sbjct: 337 YHLDEP--IGDPAAINTFLICSAAREAGVKVMLSGMGADELFAGYRKHLAN 385
>dbj|BAA89484.1| (AB029550) LtsA [Corynebacterium glutamicum]
Length = 640
Score = 118 bits (292), Expect = 2e-25
Identities = 110/380 (28%), Positives = 174/380 (44%), Gaps = 70/380 (18%)
Query: 23 KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLAMTGSKSYTQPFYNEIILVH 82
+HRGPD G W + N + +I L G + +
Sbjct: 29 RHRGPDDAGTWHDADAAFGFNRLSIIDIAHSHQPL------RWGPADEPDRY----AMTF 78
Query: 83 NGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGDY 142
NGEIYN+ LRK L + G F T D E I+ + E++ ++ LRG +
Sbjct: 79 NGEIYNYVELRKELSDLGYTFNTSGDGEPIVVGFHHW--------GESV---VEHLRGMF 127
Query: 143 AVAFFFNGKFYLF--RDPLGVRPLYYSSRGH---FASEKKVLWGLGEDAEPVLPGEIVSI 197
+A + + LF RD G++PL+Y++ H F+SEKK + + E+ L + +I
Sbjct: 128 GIAIWDTKEKSLFLARDQFGIKPLFYATTEHGTVFSSEKKTILEMAEEMNLDLGLDKRTI 187
Query: 198 SKH--------------GITRVRGFNVFSIRTQGK----------------------DYF 221
+ I+R+ ++R GK D F
Sbjct: 188 EHYVDLQYVPEPDTLHAQISRLESGCTATVRPGGKLEQKRYFKPQFPVQKVVKGKEQDLF 247
Query: 222 NGLQNLLIDSVK--VRASKKVGVLFSGGVDSSLIALIASRYS-DVILYTSGTEDS--KDV 276
+ + +L DSV+ +RA VG SGG+DS+ IA +A R++ D++ +T+G E +V
Sbjct: 248 DRIAQVLEDSVEKHMRADVTVGSFLSGGIDSTAIAPLAKRHNPDLLTFTTGFEREGYSEV 307
Query: 277 EWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDG 336
+ A A +G + + S E+ N I +IM+ +++P + +PLYF AR+
Sbjct: 308 DVAAESAAAIGAEHIVKIVSPEEYANAIPKIMWYLDDP--VADPSLVPLYFVAAEARKH- 364
Query: 337 VKVLLSGQGADELFGGYAKY 356
VKV+LSG+GADELFGGY Y
Sbjct: 365 VKVVLSGEGADELFGGYTIY 384
>pir||T50028 asparagine synthetase (ASN3) - Arabidopsis thaliana (fragment)
>gi|7960743|emb|CAB92065.1| (AL356332) asparagine
synthetase (ASN3)(fragment) [Arabidopsis thaliana]
Length = 441
Score = 104 bits (256), Expect = 3e-21
Identities = 93/317 (29%), Positives = 155/317 (48%), Gaps = 37/317 (11%)
Query: 157 DPLGVRPLYYS----SRGHFASEKKVLWGLGEDAEPVL---PGEIVSISKHGITRVRGFN 209
D +G+ PLY FASE K L +D E + PG I S + G+ R
Sbjct: 1 DAIGITPLYIGWGLDGSVWFASEMKAL---SDDCEQFMCFPPGHIYSSKQGGLRRWYNPP 57
Query: 210 VFSIRTQGKDYFN-GLQNLLIDSVKVRASKKV--GVLFSGGVDSSLIALIASRY------ 260
FS Y ++N +V R V GVL SGG+DSSL+A +A R+
Sbjct: 58 WFSEVVPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGGLDSSLVASVALRHLEKSEA 117
Query: 261 -----SDVILYTSGTEDSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPN 315
S + + G + S D++ R VA+ LG + E F+ +D + IE +++ +E +
Sbjct: 118 ACQWGSKLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFTVQDGIDAIEEVIYHVETYD 177
Query: 316 PMNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGYAKYLQNPGL------MLKDVEE 369
+ + P++ +++ + GVK++LSG+G+DE+FGGY + + P + ++
Sbjct: 178 VTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKKEFHEETCRKIKA 237
Query: 370 LGERNLARDDKVAMLNGVETRYPYLDLPFAILALNVPLELK-IRDGK---RKFILR---E 422
L + + R +K GVE R P+LD F +A+++ E K IR K++LR +
Sbjct: 238 LHQYDCLRANKSTSAWGVEARVPFLDKEFINVAMSIDPEWKMIRPDLGRIEKWVLRNAFD 297
Query: 423 IAKSLGLPEWVAEREKK 439
K+ LP+ + R+K+
Sbjct: 298 DEKNPYLPKHILYRQKE 314
>dbj|BAB05227.1| (AP001512) asparagine synthetase [Bacillus halodurans]
Length = 615
Score = 104 bits (256), Expect = 3e-21
Identities = 139/553 (25%), Positives = 229/553 (41%), Gaps = 151/553 (27%)
Query: 24 HRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLAMTGSKSYTQPFYNEIILVHN 83
HRGPD W+E+ + V + EGG Q + K YT +V+N
Sbjct: 30 HRGPDDLNVWTEKHAALGHSRLIVVDPEGG----CQPMMRERNGKRYT--------IVYN 77
Query: 84 GEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGDYA 143
GE+YN E LRK LI +G F+ D+EV+ L+ Y+ + + ++ G +A
Sbjct: 78 GELYNTEDLRKELIVKGYQFQGHSDTEVL--LVSYIEWGYQ---------CVEKFNGIFA 126
Query: 144 VAFFFNGK---FYLFRDPLGVRPLYYSSRGH---FASEKKVL------------------ 179
A +N K ++ RD LGV+PL+Y+ R FA+E K L
Sbjct: 127 FA-IWNDKDQSLFMARDRLGVKPLFYTVRHGFLLFATEIKALLAHPEIEPVLTEEGLSEV 185
Query: 180 WGLG----------EDAEPVLPGEIVSISKHG--ITRVRGFNVFSIRTQGKDYFNGLQNL 227
GLG +D + + P +++ ++G ++R + + L++L
Sbjct: 186 LGLGPSRSPGNGVFDDIQELRPAHLLTYDRNGAKVSRYWRLKSMAHSEDAMETAAHLRDL 245
Query: 228 LIDSV--KVRASKKVGVLFSGGVDSSLIALIAS------------RYS------DVILYT 267
L D+V ++ A VG+ SGGVDSS + IA+ YS D
Sbjct: 246 LEDTVERQLFADVPVGMFLSGGVDSSALTAIAALIYEREGKGLIRTYSIDYDENDKYFKA 305
Query: 268 SGTEDSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYF 327
+ + + D W +V+ ++ S E++ N+++R + + P + + LY+
Sbjct: 306 NDFQPNADGPWVEKVSTTFRTNHHNAVISIEELANQLKRAVELRDLPGMAD--VDSSLYW 363
Query: 328 STKRAREDGVKVLLSGQGADELFGGYAKYLQNPGLM-------LKDVEE----LGER--- 373
K+ ++D V V LSG+ ADE+FGGY + P +M ++ +E L ER
Sbjct: 364 FCKQIKKD-VTVGLSGECADEIFGGY-PWFHKPEVMNFNGFPWMRSADERQELLHERWRQ 421
Query: 374 --NL-------------------------ARDDKVAMLN-------------------GV 387
NL AR +++ LN +
Sbjct: 422 KLNLPKYVHDRYKETIAETPRFEEDTPEEARRREISYLNMVWFMTTLLDRKDRMSMGASL 481
Query: 388 ETRYPYLDLPFAILALNVPLELKIRDGKRKFILREIAKSLGLPEWVAEREKKA------A 441
E R P+ D A N+P E+K G+ K ILR+ + + LP+ V R+K
Sbjct: 482 EVRVPFSDHRIVEYAWNIPWEIKQFGGREKGILRKALEGI-LPDEVLYRKKSPYPKTHHP 540
Query: 442 QYGSNAQKILEKI 454
+Y Q+ +E+I
Sbjct: 541 KYTKMVQQEMERI 553
>emb|CAA07652.1| (AJ007747) putative glutamine amidotransferase [Bordetella
bronchiseptica]
Length = 636
Score = 103 bits (255), Expect = 4e-21
Identities = 104/385 (27%), Positives = 175/385 (45%), Gaps = 86/385 (22%)
Query: 23 KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLAMTGSKSYTQPFYNEI--IL 80
+HRGPD G W + Q G + L RL++ S QP ++
Sbjct: 30 QHRGPDGHGTWIDPQ---------------GRLGLAHVRLSIIDLVSGAQPMRGNSGNVI 74
Query: 81 VHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRG 140
+NGEIYN+ LR+ L E +FET D+EVILR E + + + L G
Sbjct: 75 TYNGEIYNYIELRRELGED--SFETTSDTEVILRAYE-----------KWGERCVDRLEG 121
Query: 141 DYAVAFFF--NGKFYLFRDPLGVRPLYYSS-RG--HFASEKKVLWGLGEDAEP------- 188
+A A + G+ ++ RD G++P YY+ +G +F SE K L E
Sbjct: 122 MFAFAIWDAKRGEMFVARDRFGIKPFYYACVQGGFYFGSEVKALTPFLNRVETDANALHD 181
Query: 189 -----VLPGEIVSISKHGITRVR----GFNVFSIRTQGKDYFNGLQNL--------LIDS 231
GE + G+ +++ G+ + + + Y+ +L ++
Sbjct: 182 YFAFQFCLGEKTLFA--GVRQLKPAHCGYVGRDLELKQRQYWEVQYDLDWDHTEKYFVEQ 239
Query: 232 VK----------VRASKKVGVLFSGGVDSSLIALIA------SRYSDVILYTSGTEDSKD 275
V+ +R+ +VG SGGVDSSL+A +A SR+ + + +
Sbjct: 240 VREKLQHSVDMHMRSDVEVGAYISGGVDSSLVAAMARHGRRSSRFQGFTGKFALGDAFDE 299
Query: 276 VEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYF---STKRA 332
+A+ +A+E + L + + +D N+I ++++ +++P +A P F + R
Sbjct: 300 SRYAQALADEHDILLHQVDITEDDFVNDIAKVIYHLDQP------VAGPGSFPQYAVSRL 353
Query: 333 REDGVKVLLSGQGADELFGGYAKYL 357
+D VKV+L GQG+DE+FGGYA+YL
Sbjct: 354 AKDHVKVVLGGQGSDEMFGGYARYL 378
>emb|CAC11460.1| (AL445063) asparagine synthetase related protein [Thermoplasma
acidophilum]
Length = 236
Score = 102 bits (252), Expect = 9e-21
Identities = 73/227 (32%), Positives = 120/227 (52%), Gaps = 6/227 (2%)
Query: 227 LLIDSVKVRASKKVGVLFSGGVDSSLIALIASRYSDVILYTSGTEDSKDVEWARRVAEEL 286
++ D +K + FSGG+DS+++ I+ YT+G DS D+ A V+ L
Sbjct: 9 IIRDVLKANVPAHGSIAFSGGLDSTVLMHISGYRLSA--YTAGFADSHDIARADAVSNVL 66
Query: 287 GLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGA 346
G + L + D+ I R++ I+ L S + DG +V+ +GQGA
Sbjct: 67 GFHVNHILLEKIDLMQHI-RLLKDIDGTITKQ-ETGYELILSVVLSTADGGEVV-TGQGA 123
Query: 347 DELFGGYAKYLQNPGLMLKDVEELGERNLARDDKVAMLNGVETRYPYLDLPFAILALNVP 406
DE+F GY + +++ ++ +++L ER L R+ ++A G E PYLD +A +
Sbjct: 124 DEIFYGYRRTIESMN-NVEQMKKLFERTLPREKRIAAHYGKELITPYLDSHIIDIARSFG 182
Query: 407 LELKIRDGKRKFILREIAKSLGLPEWVAEREKKAAQYGSNAQKILEK 453
+ IR+G K ++R++A+SLGLPE + KKAAQYGS + L+K
Sbjct: 183 PDHHIRNGAGKAMIRDVARSLGLPESIYSYGKKAAQYGSGIDRYLKK 229
>sp|Q10374|ASNH_MYCTU PUTATIVE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]
>gi|7477893|pir||B70785 probable asparagine synthetase -
Mycobacterium tuberculosis (strain H37RV)
>gi|1237054|emb|CAA94270.1| (Z70283) asnB [Mycobacterium
tuberculosis]
Length = 652
Score = 101 bits (250), Expect = 1e-20
Identities = 111/392 (28%), Positives = 171/392 (43%), Gaps = 81/392 (20%)
Query: 20 NLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLAMTGSKSYTQPFY---- 75
+L +HRGPD G W V GG + RL++ QP
Sbjct: 31 HLMRHRGPDESGTWHA-----------VDGASGGVVFGFN-RLSIIDIAHSHQPLRWGPP 78
Query: 76 ---NEIILVHNGEIYNHESLRKYL-IERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAI 131
+ +LV NGEIYN+ LR L + G F TD D E IL + +
Sbjct: 79 EAPDRYVLVFNGEIYNYLELRDELRTQHGAVFATDGDGEAILAGYHHWGTE--------- 129
Query: 132 KLSMKMLRGDYAVAFFFNGKFYLF--RDPLGVRPLYYSSRGH---FASEKKVLWGL---- 182
++ LRG +A A + LF RDP G++PL+ ++ ASEKK L L
Sbjct: 130 --VLQRLRGMFAFALWDTVTRELFCARDPFGIKPLFIATGAGGTAVASEKKCLLDLVELV 187
Query: 183 GEDAE------------------PVLPGEIVSISKHGITRVRGFNVFSIRTQ-------- 216
G D E L + + R+R + + T+
Sbjct: 188 GFDTEIDHRALQHYTVLQYVPEPETLHRGVRRLESGCFARIRADQLAPVITRYFVPRFAA 247
Query: 217 -------GKDYFNGLQNLLIDSVK--VRASKKVGVLFSGGVDSSLIALIASRYSD-VILY 266
+ ++ + +L DSV +RA VG SGG+DS+ IA +A R++ +I +
Sbjct: 248 SPITNDNDQARYDEITAVLEDSVAKHMRADVTVGAFLSGGIDSTAIAALAIRHNPRLITF 307
Query: 267 TSGTEDS--KDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIP 324
T+G E +++ A AE +G + + S ++ + I++ ++EP + +P
Sbjct: 308 TTGFEREGFSEIDVAVASAEAIGARHIAKVVSADEFVAALPEIVWYLDEP--VADPALVP 365
Query: 325 LYFSTKRAREDGVKVLLSGQGADELFGGYAKY 356
L+F + AR+ VKV+LSG+GADELFGGY Y
Sbjct: 366 LFFVAREARKH-VKVVLSGEGADELFGGYTIY 396
>pir||F75095 probable asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
PAB1605 - Pyrococcus abyssi (strain Orsay)
>gi|5458579|emb|CAB50067.1| (AJ248286) PUTATIVE
ASPARAGINE SYNTHETASE (GLUTAMINE-HYDROLYZING) (EC
6.3.5.4) [Pyrococcus abyssi]
Length = 611
Score = 100 bits (247), Expect = 3e-20
Identities = 117/401 (29%), Positives = 179/401 (44%), Gaps = 77/401 (19%)
Query: 1 MCLIAGGFNVSREDAIKIINLG-KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQ 59
MC I GF+ E+ +K +N +HRGPD G + + DN S G + L
Sbjct: 1 MCGI-NGFSWGDEELVKRMNDAIRHRGPDDEGVYVD------DNVSL------GHVRLAI 47
Query: 60 CRLAMTGSKSYT-QPFYNEIILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEY 118
L+ G + + E+ +V+NGEIYN +RK L E+G F ++ D+EVIL
Sbjct: 48 IDLSPKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLE 107
Query: 119 LVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLF--RDPLGVRPLYYSSRGH---FA 173
FD ++ G +A + K LF RD G++PLYY G F+
Sbjct: 108 WGFD-----------CVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFS 156
Query: 174 SEKKVLWGLGEDAEPVLPGEIV-------SISKH-------GITRVR-GFN-VFSIRT-- 215
SE K G+ + P P + V ++ H GI R+ G+N VF + T
Sbjct: 157 SEIK---GILQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRK 213
Query: 216 -QGKDYFN---------------GLQNLLIDSVKVR--ASKKVGVLFSGGVDSSLIA--- 254
+ Y++ G ++L + +VK+R A VG SGG+DSS I
Sbjct: 214 FEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAM 273
Query: 255 --LIASRYSDVILYTSGTEDSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIE 312
L+ V ++ + ++ R V +E + + F+ ED+ +I ++ E
Sbjct: 274 RHLLPDGEIKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQE 333
Query: 313 EPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGY 353
E P + Y K ARE+G+KVLL GQG+DE+ GY
Sbjct: 334 E--PFSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGY 372
>sp|O05272|ASNO_BACSU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 3
>gi|1934835|emb|CAB07965.1| (Z93940) asparagine
synthetase [Bacillus subtilis]
Length = 614
Score = 98.7 bits (242), Expect = 1e-19
Identities = 104/384 (27%), Positives = 162/384 (42%), Gaps = 81/384 (21%)
Query: 25 RGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLAMTGSKSYTQPFYNEIILVHNG 84
RGPD W E +L V +IEGG Q +YT +++NG
Sbjct: 31 RGPDDSNVWGEHHVLFGHKRLAVVDIEGGR----QPMACTYKGDTYT--------IIYNG 78
Query: 85 EIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGDYAV 144
E+YN E LRK L RG FE D+EV+L Y+ + + L G +A
Sbjct: 79 ELYNTEDLRKELRARGHQFERTSDTEVLLH--SYIEWQED---------CVDHLNGIFAF 127
Query: 145 AFFFNGKFYLF--RDPLGVRPLYYSSRGH---FASEKKV------------------LWG 181
A + + LF RD LGV+P +Y+ G F SE K ++G
Sbjct: 128 AVWDEKRNLLFAARDRLGVKPFFYTKEGSSFLFGSEIKAILAHPDIKARVDRTGLSEIFG 187
Query: 182 LGEDAEP----------VLPGEIVSISKHGITRVRGFNVFSIRTQGK--DYFNGLQNLLI 229
LG P + P ++ SK G+ R +NV S + D +++L
Sbjct: 188 LGPSRTPGTGIFKGIKEIRPAHALTFSKDGLNIWRYWNVESEKHTDSFDDTVANVRSLFQ 247
Query: 230 DSVKVRASKKVGV--LFSGGVDSSLIALIASRY------------------SDVILYTSG 269
D+V + V V SGG+DSS I IA+ + +D S
Sbjct: 248 DAVTRQLVSDVPVCTFLSGGLDSSAITAIAAGHFEKEGKAPLHTYSIDYEENDKYFQASA 307
Query: 270 TEDSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFST 329
+ + D W ++ E G + + S++D+ + +E + + P ++ ++ L+F
Sbjct: 308 FQPNDDGPWIEKMTEAFGTTHHKCVISQKDLVDHLEEAVLVKDLPGMADVDSSL-LWF-C 365
Query: 330 KRAREDGVKVLLSGQGADELFGGY 353
+ ++D V V LSG+ ADE+FGGY
Sbjct: 366 REIKKDFV-VSLSGECADEIFGGY 388
>pir||F69837 asparagine synthase homolog yisO - Bacillus subtilis
>gi|2145380|emb|CAA70643.1| (Y09476) YisO [Bacillus
subtilis] >gi|2633415|emb|CAB12919.1| (Z99109) alternate
gene name: yucB~similar to asparagine synthase [Bacillus
subtilis]
Length = 591
Score = 98.7 bits (242), Expect = 1e-19
Identities = 104/384 (27%), Positives = 162/384 (42%), Gaps = 81/384 (21%)
Query: 25 RGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLAMTGSKSYTQPFYNEIILVHNG 84
RGPD W E +L V +IEGG Q +YT +++NG
Sbjct: 8 RGPDDSNVWGEHHVLFGHKRLAVVDIEGGR----QPMACTYKGDTYT--------IIYNG 55
Query: 85 EIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGDYAV 144
E+YN E LRK L RG FE D+EV+L Y+ + + L G +A
Sbjct: 56 ELYNTEDLRKELRARGHQFERTSDTEVLLH--SYIEWQED---------CVDHLNGIFAF 104
Query: 145 AFFFNGKFYLF--RDPLGVRPLYYSSRGH---FASEKKV------------------LWG 181
A + + LF RD LGV+P +Y+ G F SE K ++G
Sbjct: 105 AVWDEKRNLLFAARDRLGVKPFFYTKEGSSFLFGSEIKAILAHPDIKARVDRTGLSEIFG 164
Query: 182 LGEDAEP----------VLPGEIVSISKHGITRVRGFNVFSIRTQGK--DYFNGLQNLLI 229
LG P + P ++ SK G+ R +NV S + D +++L
Sbjct: 165 LGPSRTPGTGIFKGIKEIRPAHALTFSKDGLNIWRYWNVESEKHTDSFDDTVANVRSLFQ 224
Query: 230 DSVKVRASKKVGV--LFSGGVDSSLIALIASRY------------------SDVILYTSG 269
D+V + V V SGG+DSS I IA+ + +D S
Sbjct: 225 DAVTRQLVSDVPVCTFLSGGLDSSAITAIAAGHFEKEGKAPLHTYSIDYEENDKYFQASA 284
Query: 270 TEDSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFST 329
+ + D W ++ E G + + S++D+ + +E + + P ++ ++ L+F
Sbjct: 285 FQPNDDGPWIEKMTEAFGTTHHKCVISQKDLVDHLEEAVLVKDLPGMADVDSSL-LWF-C 342
Query: 330 KRAREDGVKVLLSGQGADELFGGY 353
+ ++D V V LSG+ ADE+FGGY
Sbjct: 343 REIKKDFV-VSLSGECADEIFGGY 365
>gi|6323542 Yml096wp [Saccharomyces cerevisiae]
>gi|2494766|sp|Q04489|YMJ6_YEAST HYPOTHETICAL 59.5 KD
PROTEIN IN VPS9-RAD10 INTERGENIC REGION
>gi|1077289|pir||S49630 probable membrane protein
YML096w - yeast (Saccharomyces cerevisiae)
>gi|575706|emb|CAA86641.1| (Z46660) unknown orf, len:
525, CAI: 0.12, some similarity to asparagine
synthetases (32.8% identity in 122 aa overlap)
[Saccharomyces cerevisiae]
Length = 525
Score = 97.9 bits (240), Expect = 2e-19
Identities = 127/460 (27%), Positives = 194/460 (41%), Gaps = 104/460 (22%)
Query: 69 SYTQPFYNEII-------LVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVF 121
S QPF + I L NGE+YN E I +G D DS I +L+
Sbjct: 73 SLRQPFTKQSINVDDRYFLQFNGELYNKE------ISQG-----DNDSLYIASMLQ---- 117
Query: 122 DRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYSSR---------- 169
LK + +K L G+YA + + K Y RDP+G R L YS
Sbjct: 118 --NLKEGMGVIDVIKSLEGEYAYTIYDVNSSKLYFGRDPIGRRSLSYSVTPDNELYVASV 175
Query: 170 -GHFASEKKVLWGLGEDAEP---VLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFNGLQ 225
G S + + G+ + + +L S + +T N S+ K+ L
Sbjct: 176 TGSAGSFQDCIGGVIYEYDTRTKLLNSNQRSHLPYEVTSEIDLNFTSLSEVSKN----LY 231
Query: 226 NLLIDSVKVRA---------SKKVGVLFSGGVDSSLI-ALIASRYSD--------VI--- 264
+L DSVK R + + VLFSGG+D S+I ALI + VI
Sbjct: 232 AVLRDSVKKRVESIHPRHIENSPIAVLFSGGIDCSVIVALICEVLQENDYKCGKPVIELL 291
Query: 265 ----------LYTSGTEDSK----DVEWARRVAEELGLKLRE-SLFSREDIENEIERIMF 309
L+ S T D K + + + + +KL E + E ++ + I
Sbjct: 292 NVSFENPRTGLFPSDTPDRKLSINSAKTLQNLYPNVDIKLVEVDVPYDEYLKWKPFVINL 351
Query: 310 AIEEPNPMNLAIAIPLYFSTKRA-------------REDGVKVLLSGQGADELFGGYAKY 356
+ M+L+IAI +F+++ + G+ VL SG GADEL+GGY K+
Sbjct: 352 MYPKQTEMDLSIAIAFFFASRGRGFLTSLNGERTPYQRHGI-VLFSGLGADELYGGYHKF 410
Query: 357 LQNPGLML-----KDVEELGERNLARDDKVAMLNGVETRYPYLD-LPFAILALNVPLELK 410
P L + + + +RNL RDDKV NGVE RYP+LD + +P+ K
Sbjct: 411 ANKPPHELVEELTRQINNIYDRNLNRDDKVIAHNGVEVRYPFLDEYVIKLSTAEIPINFK 470
Query: 411 IRDGKRKFILREIAKSLGLPEWVAEREKKAAQYGSNAQKI 450
+ K ILR++A + ++ K+A Q+G+ + K+
Sbjct: 471 V----NKLILRKVASQYLKLDGISSEPKRAIQFGAKSAKM 506
>pir||A83638 probable glutamine amidotransferase PA0051 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9945874|gb|AAG03441.1|AE004445_2 (AE004445) probable
glutamine amidotransferase [Pseudomonas aeruginosa]
Length = 610
Score = 88.5 bits (216), Expect = 1e-16
Identities = 100/390 (25%), Positives = 162/390 (40%), Gaps = 90/390 (23%)
Query: 25 RGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLAMTGSKSYTQPFYNEIILVHNG 84
RGPD+ G W R L V ++ GG + SY P E+ LV+ G
Sbjct: 31 RGPDAEGIWKHRNALLGHRRLAVIDLSGGVQPM-----------SYRFPTGQEVTLVYTG 79
Query: 85 EIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGDYAV 144
E+YNH++LR+ L G F T D+EV+L YL + + + L G +A
Sbjct: 80 EVYNHDALRERLRRAGHEFRTRSDTEVVLH--AYLQWGER---------CCEYLTGMFAF 128
Query: 145 AFF--FNGKFYLFRDPLGVRPLYYSSRGH---FASEKKVLWGLGE--------------- 184
A F +G L RD LG++PLYY+ F SE K + E
Sbjct: 129 AVFDGRDGHLLLVRDRLGIKPLYYARHREGLLFGSEIKSILAHPEFAARLDAVGLVDLLT 188
Query: 185 -----------DAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFNGLQNLLIDS-- 231
+ + +LPG ++S + ++R + + +R Q ++ + LQ+ + +
Sbjct: 189 LSRGTSQTPFREVQELLPGHLLSWRPNSQAKLRRY--WEVRRQ--EHADDLQSTVQRTRE 244
Query: 232 -------VKVRASKKVGVLFSGGVDSSLIALIASRYS------DVILY------------ 266
++ A V L SGG+DS+ + IA R + D+ +
Sbjct: 245 LVTRALGAQLHADVPVCSLLSGGLDSTALTGIAQRIAKAEHGGDINSFSVDFVGQAEQFR 304
Query: 267 TSGTEDSKDVEWARRVAEELGLKLRESLFSREDI--ENEIERIMFAIEEPNPM-NLAIAI 323
+ +D +A A+ +G + R L ++ E E + A + P ++ ++
Sbjct: 305 SDDLRPDQDQPFALLAAQYIGSRHRTVLIDNAELVCERAREEVFRAKDVPFTFGDMDTSL 364
Query: 324 PLYFSTKRAREDGVKVLLSGQGADELFGGY 353
L F R V +SG+GADELFGGY
Sbjct: 365 HLMFGEIRRHS---TVAISGEGADELFGGY 391
>pir||B75212 amidophosphoribosyltransferase (EC 2.4.2.14) PAB0151 precursor
[similarity] - Pyrococcus abyssi (strain Orsay)
>gi|5457654|emb|CAB49145.1| (AJ248283)
amidophosphoribosyltransferase (purF) [Pyrococcus
abyssi]
Length = 447
Score = 86.2 bits (210), Expect = 7e-16
Identities = 69/212 (32%), Positives = 103/212 (48%), Gaps = 22/212 (10%)
Query: 6 GGFNVSREDAIKIINLG----KHRGPDSFGF--WSER------QMLKSDNFSDVSEIEGG 53
G F VS EDA++ G +HRG + G W+ R L S+ F + S
Sbjct: 12 GIFGVSSEDAVRKTYYGLIALQHRGQEGAGISVWNGRIRTIKGHGLVSEVFDENSLNLAS 71
Query: 54 EIALIQCRLAMTGSKSYTQPFY-----NEIILVHNGEIYNHESLRKYLIERGVAFETDVD 108
IA+ R + +GS S QP E+ + HNG + N LR+ G+ F + VD
Sbjct: 72 NIAIGHVRYSTSGSLSEVQPLEVRCCGYELAIAHNGTLTNFIPLRRLYEGMGIKFHSSVD 131
Query: 109 SEVILRLLEYLVFDRKLKVE-EAIKLSMKMLRGDYAVAFFFNGKFYLFRDPLGVRPLYYS 167
+EVI + +L ++K E EA++ + ++G Y++ F+GK RDP+G RPL +
Sbjct: 132 TEVIG--ISFLNHYSQVKDEFEAMRRVFEEVKGAYSILILFDGKIIAARDPVGFRPLVFG 189
Query: 168 SRG--HFASEKKVLWGLGEDAEPVLPGEIVSI 197
+FASE L G + VLPGE+ I
Sbjct: 190 EGDGYYFASEDSALRMFGLETRDVLPGEVFVI 221
>dbj|BAB12569.1| (AB039379) asparagine synthase homolog [Streptomyces aureofaciens]
Length = 609
Score = 85.8 bits (209), Expect = 1e-15
Identities = 104/395 (26%), Positives = 164/395 (41%), Gaps = 94/395 (23%)
Query: 25 RGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLAMTGSKSYTQPFYNE-----II 79
RGPD+ G W G L RL++ + QP E
Sbjct: 31 RGPDAEGLWF-----------------GRAAGLGHRRLSVIDPEHGRQPMTAEHEGRGAA 73
Query: 80 LVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLR 139
+ +GEIYN LR L G F T D+EV+LR YL + L ++ L
Sbjct: 74 ITFSGEIYNFRELRAELTSHGHRFRTSCDTEVVLR--GYLQWGAAL---------VERLN 122
Query: 140 GDYAVAFF--FNGKFYLFRDPLGVRPLYY---SSRGHFASEKKVLWG-------LGEDA- 186
G +A A + + + L RD +GV+PLYY ++ F SE K + +G D
Sbjct: 123 GMFAFAVWDEHSEELLLVRDRMGVKPLYYWPTAAGVRFGSEPKAVLADRTLSRRVGPDGL 182
Query: 187 ------------------EPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFNGL---- 224
V PG++V + + G+ R + R + +++ + L
Sbjct: 183 CEVLDMVKTPEAAVFSGLVEVRPGQLVRVGRGGVRRQTYW-----RLEAREHTDDLPTTI 237
Query: 225 ---QNLLIDSV--KVRASKKVGVLFSGGVDSSLIALIASRYSDVILYT---SGTEDS--- 273
++LL D+V ++ + +G L SGG+DSS + +A+R V ++ G D+
Sbjct: 238 GTVRDLLADTVTRQLVSDVPLGTLLSGGLDSSAVTALAARSGPVRSFSVDFRGAADAFAS 297
Query: 274 ------KDVEWARRVAEELGLKLRESLFSREDIENEIER--IMFAIEEPNPMNLAIAIPL 325
D + R +A +G E L + + R ++ A + P P P
Sbjct: 298 DAVRREADAPYVRELAGHVGADHLEVLLDSPGLSDPAVRSAVLRATDLP-PAYWGDMWPS 356
Query: 326 YFSTKRAREDGVKVLLSGQGADELFGGYAKYLQNP 360
+ RA + V V LSG+ ADELFGGY ++ +NP
Sbjct: 357 LYLFFRAVRENVTVALSGEAADELFGGY-RWFRNP 390
>pir||JQ2245 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) - garden
asparagus
Length = 590
Score = 83.9 bits (204), Expect = 4e-15
Identities = 102/372 (27%), Positives = 152/372 (40%), Gaps = 79/372 (21%)
Query: 4 IAGGFNVSREDAIKIINLG---KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQC 60
+ G + S+ ++++ L KHRGPD G + G+ L
Sbjct: 7 VLGCSDDSQAKRVRVLELSRRLKHRGPDWSGL-----------------CQHGDCFLSHQ 49
Query: 61 RLAMTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLE 117
RLA+ S QP YNE I++ NGEIYNHE LR+ L + + T D EVI L E
Sbjct: 50 RLAIIDPASGDQPLYNEDKSIVVTVNGEIYNHEELRRRLPDH--KYRTGSDCEVIAHLYE 107
Query: 118 YLVFDRKLKVEEAIKLSMKMLRGDYAVAFFF--NGKFYLFRDPLGVRPLYYS----SRGH 171
E + + ML G ++ N F RD +G+ PLY
Sbjct: 108 -----------EHGEDFVDMLDGMFSFVLLDTRNNCFVAARDAVGITPLYIGWGLDGSVW 156
Query: 172 FASEKKVLWGLGEDAEPVLPGEIVSISKHGITRVRGFNVFSIRTQGKDYFN-GLQNLLID 230
+SE K L E E PG + S R ++ Y L+ D
Sbjct: 157 LSSEMKGLNDDCEHFEVFPPGNLYSSRSGSFRRWYNPQWYNETIPSAPYDPLVLRKAFED 216
Query: 231 SVKVRASKKV--GVLFSGGVDSSLIALIASRYSDVILYTSGTEDSKDVEWARRVAEELGL 288
+V R V GVL SGG+DSSL+A + +R + +G+ + AE+ G
Sbjct: 217 AVIKRLMTDVPFGVLLSGGLDSSLVAAVTAR------HLAGS----------KAAEQWGT 260
Query: 289 KLRESLFSREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGADE 348
+L F + +L A L ++ + GVK+++SG+G+DE
Sbjct: 261 QLHS----------------FCVGLEGSPDLKAAKEL--MARKIKSLGVKMVISGEGSDE 302
Query: 349 LFGGYAKYLQNP 360
+FGGY + + P
Sbjct: 303 IFGGYLYFHKAP 314
>pir||C72508 hypothetical protein APE2041 - Aeropyrum pernix (strain K1)
>gi|5105739|dbj|BAA81051.1| (AP000063) 351aa long
hypothetical protein [Aeropyrum pernix]
Length = 351
Score = 81.1 bits (197), Expect = 2e-14
Identities = 71/242 (29%), Positives = 117/242 (48%), Gaps = 17/242 (7%)
Query: 227 LLIDSVKVRASKKVG-VLFSGGVDSSLIALIASRYSDVILYTSGTEDSKDVEWARRVAEE 285
L + + R S+ G + SGG+D+S +A + + + +T S DV +AR AE
Sbjct: 44 LRLAEIVARESRGCGCITLSGGIDTSFVAAVHPKRRVLRAFTVDLGGS-DVRYARLAAER 102
Query: 286 LGLKLRESLF-SREDIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQ 344
LGLK + +D ++ ++ ++E +P+ +A + Y + A +G +LSG
Sbjct: 103 LGLKDHIIVNPGLDDFSKAVDWVLRSMEVIDPVEVAASSIHYIALSAAAGEGCGCVLSGD 162
Query: 345 GADELFGGYAKYLQNPGLMLKDVEELGE--RNLARDDK-----VAMLNGVETRYPYLDLP 397
G DELF GY+ L K + L E R +AR+ + VA + GV+ P
Sbjct: 163 GGDELFLGYSFLLD------KSIGYLEEWRRRMAREARLPTVEVARMLGVDVIAPLYSRE 216
Query: 398 FAILALNVPLELKIRDGKRKFILREIAKSLGLPEWVAEREKKAAQYGSNAQKILEKIAKS 457
LAL PLE ++ K+++R + LGL E +A R+K GS + L+ +A +
Sbjct: 217 SRRLALETPLECLVKGRWGKWMIRMLLDELGLRE-IAFRDKAPVTEGSGSLAALKSLATA 275
Query: 458 RG 459
G
Sbjct: 276 MG 277
>emb|CAC12366.1| (AL445067) probable amidophosphoribosyltransferase [Thermoplasma
acidophilum]
Length = 474
Score = 81.1 bits (197), Expect = 2e-14
Identities = 65/213 (30%), Positives = 102/213 (47%), Gaps = 22/213 (10%)
Query: 13 EDAIKIINLGKHRGPDSFG---FWSERQMLK------SDNFSDVSEIEGGEIALIQCRLA 63
E I + +HRG +S G F + +LK +D F+ ++ G++ + R +
Sbjct: 33 EPIITALRTLQHRGQESAGMAVFDGRKTVLKKGRGLVTDVFNPATDSIKGKVGVGHTRYS 92
Query: 64 MTGSKSYTQ--PFYNE-----IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLL 116
GSK PF I L HNGEI N + L++ + + GV F++D D+EV+L L
Sbjct: 93 TAGSKDVIDAGPFVINSQVGFISLSHNGEIVNADELKEEMKKEGVNFQSDSDTEVLLAEL 152
Query: 117 EYLVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLFRDPLGVRPLYYSSRGH---FA 173
+ K +++ ++SM LRG YA A N + + RDP G+RPL A
Sbjct: 153 SRNI--SKYGLKKGFEVSMNTLRGAYACAVGINDRLFAMRDPNGIRPLIVGKNADGYIIA 210
Query: 174 SEKKVLWGL-GEDAEPVLPGEIVSISKHGITRV 205
SE + L G V PGE+V ++ G+ +
Sbjct: 211 SESCAIDALDGTIIRNVEPGEVVELTDDGLKTI 243
>gb|AAD38229.1| (U17224) CarA [Pectobacterium carotovorum]
Length = 503
Score = 78.8 bits (191), Expect = 1e-13
Identities = 67/227 (29%), Positives = 110/227 (47%), Gaps = 14/227 (6%)
Query: 240 VGVLFSGGVDSSLIALIASR-YSDVILYTSGTEDSKDVEWARRVAEELGLKLRESLFSRE 298
VG+ SGG+DSSL+ +ASR + + Y+ GTE S + E++++VA+ LG + + S
Sbjct: 241 VGIPLSGGLDSSLVTALASRHFKKLNTYSIGTELSNEFEFSQQVADALGTHHQMKILSET 300
Query: 299 DIENEIERIMFAIEEPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGYAKYLQ 358
++ N I ++ E + ++ I L F+ R + V +L+G G+D LFGG K
Sbjct: 301 EVINGIIESIYYNEIFDGLSAEIQSGL-FNVYRQAQGQVSCMLTGYGSDLLFGGILK--- 356
Query: 359 NPGLMLKDVEELGERNLARD-------DKVAMLNGVETRYPYLDLPFAILALNVPLELKI 411
PG + +L + R A G++ R+P+ L + + KI
Sbjct: 357 -PGAQYDNPNQLLAEQVYRTRWTGEFATHGASCYGIDIRHPFWSHSLISLCHALHPDYKI 415
Query: 412 RDGKRKFILREIAKSLG-LPEWVAEREKKAAQYGSNAQKILEKIAKS 457
D + K ILRE A SL LP+ + R+K GS+ + + S
Sbjct: 416 FDNEVKNILREYADSLQLLPKDIVWRKKIGIHEGSSVNQAFANVLGS 462
>pir||A72277 amidophosphoribosyltransferase (EC 2.4.2.14) TM1247 [similarity] -
Thermotoga maritima (strain MSB8)
>gi|4981802|gb|AAD36322.1|AE001780_6 (AE001780)
amidophosphoribosyltransferase [Thermotoga maritima]
Length = 431
Score = 77.6 bits (188), Expect = 3e-13
Identities = 72/224 (32%), Positives = 101/224 (44%), Gaps = 24/224 (10%)
Query: 1 MCLIAGGFNVSREDAIKIIN---LG-KHRGPDSFGFWSER-QMLKSDNFSDV----SEIE 51
MC IAG +NV +DA +++ LG +HRG +S G + + +K D E
Sbjct: 1 MCGIAGVWNV--KDAFSVLHDVLLGLQHRGQESVGVVVDGFKTIKGKGLVDTVLTEDRWE 58
Query: 52 GGEIALIQCRLAMTGSKSYTQPFY-----NEIILVHNGEIYNHESLRKYLIERGVAFETD 106
E + R + GS QP + + HNG I N E + L E+G F++
Sbjct: 59 DAEKGIGHVRYSTAGSLEDIQPIVAFTRKGRLAIAHNGNIPNGEKWIEMLKEKGAVFQSS 118
Query: 107 VDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLFRDPLGVRPLYY 166
+DSEV L L+ D K E+I ++K + Y++ RDP GVRPL+Y
Sbjct: 119 LDSEVFLHLISMSEGDLK----ESIVKALKKVPLAYSLLILHEEFLAAARDPYGVRPLFY 174
Query: 167 SSRGH---FASEKKVLWGLG-EDAEPVLPGEIVSISKHGITRVR 206
G ASE L +G ED E V G +V S G VR
Sbjct: 175 GKYGDGIVVASEDAALKAIGVEDIEEVPSGTVVFFSNKGTETVR 218
>sp|O26742|PUR1_METTH AMIDOPHOSPHORIBOSYLTRANSFERASE PRECURSOR (GLUTAMINE
PHOSPHORIBOSYLPYROPHOSPHATE AMIDOTRANSFERASE) (ATASE)
(GPATASE) >gi|7433952|pir||H69185
amidophosphoribosyltransferase (EC 2.4.2.14) MTH646
precursor [similarity] - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2621728|gb|AAB85151.1| (AE000845)
amidophosphoribosyltransferase [Methanobacterium
thermoautotrophicum]
Length = 474
Score = 76.9 bits (186), Expect = 5e-13
Identities = 53/158 (33%), Positives = 81/158 (50%), Gaps = 12/158 (7%)
Query: 53 GEIALIQCRLAMTGSKSY--TQPFYNE-----IILVHNGEIYNHESLRKYLIERGVAFET 105
G + + R + TG +QPF++E I + HNG+I N LR+ L E G F +
Sbjct: 77 GNVGIGHVRYSTTGESRIENSQPFWSEFQGGKIAIAHNGDIINSMELREELEEEGHNFVS 136
Query: 106 DVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLFRDPLGVRPLY 165
DSEVI LL +D K + +IK + L G Y++ N Y+ RDP+G++PL
Sbjct: 137 TTDSEVICHLLSR-EYDEKPNMIYSIKRVSEQLVGSYSLVVLLNQDLYVVRDPVGIKPLA 195
Query: 166 YSSRGH---FASEKKVLWGLG-EDAEPVLPGEIVSISK 199
++ +G ASE +G E V PGEI+ +++
Sbjct: 196 FARKGSTQIVASETVAFDVIGAEHVRDVQPGEILHLNR 233
>pir||A70401 amidophosphoribosyltransferase (EC 2.4.2.14) purF [similarity] -
Aquifex aeolicus >gi|2983634|gb|AAC07202.1| (AE000727)
amidophosphoribosyltransferase [Aquifex aeolicus]
Length = 465
Score = 76.9 bits (186), Expect = 5e-13
Identities = 73/245 (29%), Positives = 115/245 (46%), Gaps = 37/245 (15%)
Query: 1 MCLIAGGFN--VSREDAIKIINLGKHRGPDSFGFWSERQ-----------MLKSDNFSDV 47
MC +AG FN + E A+ +I +HRG +S G S + +L++ D+
Sbjct: 1 MCGVAGVFNSPYAAEYALLMIYSLQHRGQESVGISSSDEKEIYTVKKPGRVLEAIKKEDL 60
Query: 48 SEIEGGEIALIQCRLAMTGSKS--YTQPFYNE-----IILVHNGEIYNHESLRKYLIERG 100
+++G IA+ R + G+ QP E + +VHNG + N +LRK L ++G
Sbjct: 61 EKLKGS-IAIAHVRYSTAGNAGGFNAQPLVRETPLGKVAVVHNGNLVNFLTLRKELEDKG 119
Query: 101 VAFETDVDSEVILRLLE----YLVFDRKLKVEEA-----IKLSMKMLRGDYAVAFFFNGK 151
+ F + DSE+ L LLE Y+ L E+ I ++ ++G Y++ + F K
Sbjct: 120 IKFNYNSDSELFLALLEEGRTYIPEGVTLHPEDKDFLPYIFYALSRVKGAYSLIYLFRDK 179
Query: 152 FYLFRDPLGVRPLYYSSRGH---FASEKKVLWGL-GEDAEPVLPGEIVSISKHGITRVRG 207
+ RDP G RPL + + ASE L E V PGE++ I K G R
Sbjct: 180 LVVARDPYGFRPLALGRKKNTILVASESCAFDILEAEFWREVKPGEVLVIDKKG---KRS 236
Query: 208 FNVFS 212
+ +FS
Sbjct: 237 YRIFS 241
>pir||T40508 probable asparagine synthase - fission yeast (Schizosaccharomyces
pombe) >gi|3560144|emb|CAA20731.1| (AL031534) putative
asparagine synthase [Schizosaccharomyces pombe]
Length = 548
Score = 76.9 bits (186), Expect = 5e-13
Identities = 50/136 (36%), Positives = 73/136 (52%), Gaps = 13/136 (9%)
Query: 338 KVLLSGQGADELFGGYAKYL---QNPGL------MLKDVEELGERNLARDDKVAMLNGVE 388
KVL+SG GADE GGY ++L + G+ + D++ + RNL RDD+V G E
Sbjct: 408 KVLISGLGADEQLGGYMRHLRAFERKGMAGLEEELQLDIDRIPHRNLGRDDRVMSNQGKE 467
Query: 389 TRYPYLDLPFAILALNVPLELKIR---DGKRKFILREIAKSLGLPEWVAEREKKAAQYGS 445
RYP+LD L + K+R G K ILRE+ + LG P ++ +K+A Q+GS
Sbjct: 468 VRYPFLDERLMALFSKMKTTDKMRLDLVGGDKLILRELGRRLGCP-LASQEKKRAIQFGS 526
Query: 446 NAQKILEKIAKSRGVK 461
A K+ ++ G K
Sbjct: 527 KAAKMTPGTGRTSGRK 542
>sp|O57979|PUR1_PYRHO AMIDOPHOSPHORIBOSYLTRANSFERASE PRECURSOR (GLUTAMINE
PHOSPHORIBOSYLPYROPHOSPHATE AMIDOTRANSFERASE) (ATASE)
(GPATASE) >gi|7433951|pir||A71248
amidophosphoribosyltransferase (EC 2.4.2.14) PH0240
precursor [similarity] - Pyrococcus horikoshii
>gi|3256629|dbj|BAA29312.1| (AP000001) 449aa long
hypothetical amidophosphoribosyltransferase [Pyrococcus
horikoshii]
Length = 449
Score = 75.7 bits (183), Expect = 1e-12
Identities = 66/215 (30%), Positives = 102/215 (46%), Gaps = 27/215 (12%)
Query: 6 GGFNVSREDAIKIINLG----KHRGPDSFGF--WS-ERQMLKSDNFSDVSEI-EGGEIAL 57
G F +DA K G +HRG + G W + + +K VSE+ +GG I
Sbjct: 11 GIFGAYSQDATKKTYYGLMALQHRGQEGAGISVWDGDIRTVKGHGL--VSEVFKGGSIRR 68
Query: 58 IQ-------CRLAMTGSKSYTQPFYNE-----IILVHNGEIYNHESLRKYLIERGVAFET 105
+ R + +GS S QP E + + HNG + N LR++ RG F +
Sbjct: 69 LNGNPVIGHVRYSTSGSLSEVQPLEVECCGYKVSIAHNGTLTNFLPLRRFYESRGFKFRS 128
Query: 106 DVDSEVILRLLEYLVFDRKLKVE-EAIKLSMKMLRGDYAVAFFFNGKFYLFRDPLGVRPL 164
+D+EVI + +L +LK E EA+ + ++G Y+V FNGK RDP+G RPL
Sbjct: 129 SIDTEVIA--VSFLNHYSELKDEFEAMSRVFEEVKGAYSVLMLFNGKLIAVRDPVGFRPL 186
Query: 165 YYSSRG--HFASEKKVLWGLGEDAEPVLPGEIVSI 197
+ + +F+SE L + V PGE++ +
Sbjct: 187 SFGAGDGYYFSSEDSALRMFCTNIRDVSPGEVIVV 221
>sp|Q57657|PUR1_METJA PROBABLE AMIDOPHOSPHORIBOSYLTRANSFERASE (GLUTAMINE
PHOSPHORIBOSYLPYROPHOSPHATE AMIDOTRANSFERASE) (ATASE)
(GPATASE) >gi|2127755|pir||E64325
amidophosphoribosyltransferase (EC 2.4.2.14) MJ0204
[similarity] - Methanococcus jannaschii
>gi|1590947|gb|AAB98188.1| (U67476)
amidophosphoribosyltransferase (purF) [Methanococcus
jannaschii]
Length = 471
Score = 75.3 bits (182), Expect = 1e-12
Identities = 68/222 (30%), Positives = 103/222 (45%), Gaps = 26/222 (11%)
Query: 1 MCLIAGGFNVSREDAIKIINLG----KHRGPDSFGFWSE--RQMLKSDNFSDVSEIEGGE 54
MC I G ++ R + K I G +HRG + G + + + N V+++ E
Sbjct: 1 MCGIFGIYSYERLNVAKKIYYGLFALQHRGQEGAGIATSDGKNIHYYKNIGLVTDVFKNE 60
Query: 55 --------IALIQCRLAMTGSKSYT--QPF-----YNEIILVHNGEIYNHESLRKYLIER 99
I + R + TG K+ QPF + I + HNG++ N + LR+ L +
Sbjct: 61 TLQNLFGYIGIGHVRYSTTGGKAVENCQPFVVKSSFGNIAIAHNGDLVNSDELRRELEMK 120
Query: 100 GVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLFRDPL 159
G F + DSEVI +LL + K+ EAIK ++K L G Y++ FN RDP
Sbjct: 121 GHIFTSSTDSEVIAQLLVRELLKTSDKI-EAIKNTLKKLVGAYSLLIMFNDSLIAVRDPW 179
Query: 160 GVRPLYY---SSRGHFASEKKVLWGL-GEDAEPVLPGEIVSI 197
G +PL S + +SE L L E + + PGEI+ I
Sbjct: 180 GFKPLCIGRDESNIYISSEDCALTTLDAEFVKDIEPGEIIEI 221
>pir||A75545 amidophosphoribosyltransferase (EC 2.4.2.14) DR0220 precursor
[similarity] - Deinococcus radiodurans (strain R1)
>gi|6457891|gb|AAF09806.1|AE001884_1 (AE001884)
amidophosphoribosyltransferase [Deinococcus radiodurans]
Length = 477
Score = 74.5 bits (180), Expect = 2e-12
Identities = 64/230 (27%), Positives = 102/230 (43%), Gaps = 32/230 (13%)
Query: 2 CLIAGGFNVSREDAIKIINLG----KHRGPDSFG--------FWSERQM-----LKSDNF 44
C + G ++ D + LG +HRG ++ G F E+ + + +
Sbjct: 19 CGVFGIYSAQPNDLAWLTYLGLFALQHRGQEAAGMCVSDGDRFHVEKDLGLVTQVFDERR 78
Query: 45 SDVSEIEGGEIALIQCRLAMTGS--KSYTQPFYNE-----IILVHNGEIYNHESLRKYLI 97
D + G ++++ R + TGS + QP + + HNG N +R+ ++
Sbjct: 79 LDSVRLAGAQVSIGHVRYSTTGSNLRFNAQPLTTRTNKGILGMAHNGNFVNALEVRREML 138
Query: 98 ERGVAFETDVDSEVILRLLEYLVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLFRD 157
E G F T DSEV+L L+ + ++ + EA +M+ LRG YA + FRD
Sbjct: 139 EEGALFATTNDSEVMLNLIAR---ESRMDLVEATAAAMQRLRGGYACVLMSRSQLLGFRD 195
Query: 158 PLGVRPLYYSSRGH----FASEKKVLWGLGED-AEPVLPGEIVSISKHGI 202
P GVRPL R ASE L+ +G V PGE+V I + G+
Sbjct: 196 PHGVRPLVIGQREDGAWVLASEPCALYAIGARLIRDVAPGELVWIDREGL 245
>pir||T42010 probable asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) -
fission yeast (Schizosaccharomyces pombe) (fragment)
>gi|1749410|dbj|BAA13764.1| (D89101) similar to
Saccharomyces cerevisiae Asparagine
synthetase(glutamine-hydrolyzing)2, SWISS-PROT Accession
Number P49090 [Schizosaccharomyces pombe]
Length = 559
Score = 71.0 bits (171), Expect = 3e-11
Identities = 88/303 (29%), Positives = 134/303 (44%), Gaps = 48/303 (15%)
Query: 4 IAGGFNVSREDAIKIINLGKHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQCRLA 63
+A + A+ + +HRGPD WS + + + L RLA
Sbjct: 12 VAEDIEAFKPKALHLSKQLRHRGPD----WSGKAIRN-------------QTILCHERLA 54
Query: 64 MTGSKSYTQPFYNE---IILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEYLV 120
+ G +S QP ++ ++L NGEIYNH LR+ L + F+T D EVIL L
Sbjct: 55 IVGVESGAQPLVSDDGKLVLTVNGEIYNHLKLRENL-KGNYKFKTYSDCEVILYLYR--- 110
Query: 121 FDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLF--RDPLGVRPLY--YSS----RGHF 172
E ML G ++ + K + RDP+G+ LY +SS +F
Sbjct: 111 --------EHGPACANMLDGMFSWVLYDQDKDKVVAARDPIGITTLYQGFSSDSPDTAYF 162
Query: 173 ASEKKVLWGLGEDAEPVLPGEIVSISKHGITRV---RGFNVFSIRTQGKDYFNGLQNLLI 229
ASE K L + + PG R ++ I + DY L+ L
Sbjct: 163 ASELKALHPVCDKIIAFPPGHYYDSETKQTVRYFKPSWWDENKIPSNPVDY-KLLRETLE 221
Query: 230 DSVKVRASKKV--GVLFSGGVDSSLIALIASRYSDVIL-YTSGTEDSKDV-EWARRVAEE 285
SV+ R +V GVL SGG+DSSLIA IA+R ++ + TS +E+++ + W + +
Sbjct: 222 ASVRKRLMAEVPYGVLLSGGLDSSLIASIAARETEKLANSTSQSEEARTITAWPKLHSFA 281
Query: 286 LGL 288
+GL
Sbjct: 282 IGL 284
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.320 0.140 0.397
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 173396606
Number of Sequences: 2977
Number of extensions: 7296766
Number of successful extensions: 19936
Number of sequences better than 1.0e-10: 86
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 80
Number of HSP's that attempted gapping in prelim test: 19590
Number of HSP's gapped (non-prelim): 120
length of query: 479
length of database: 189,106,746
effective HSP length: 57
effective length of query: 422
effective length of database: 154,992,987
effective search space: 65407040514
effective search space used: 65407040514
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 167 (69.5 bits)