BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB0811 (rad32) DE:DNA REPAIR PROTEIN RAD32 (RAD32) (423 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||E75103 DNA repair protein rad32 (rad32) PAB0811 - Pyrococcu... 873 0.0 pir||D71083 probable phosphoesterase (EC 3.1.-.-) PHAR032 - Pyro... 628 e-179 gi|11498636 DNA repair protein RAD32 (rad32) [Archaeoglobus fulg... 246 3e-64 gb|AAK42418.1| DNA repair protein (rad32/mre11) [Sulfolobus solf... 118 1e-25 pir||B64465 probable phosphoesterase (EC 3.1.-.-) MJ1323 - Metha... 116 6e-25 pir||E69171 conserved hypothetical protein MTH541 - Methanobacte... 104 2e-21 emb|CAC11305.1| (AL445063) conserved hypothetical protein [Therm... 80 4e-14 pir||E70462 ATP-dependent dsDNA exonuclease (EC 3.1.-.-) - Aquif... 79 1e-13 gb|AAG19044.1| (AE005003) phosphoesterase; Ppe [Halobacterium sp... 77 4e-13 >pir||E75103 DNA repair protein rad32 (rad32) PAB0811 - Pyrococcus abyssi (strain Orsay) >gi|5458642|emb|CAB50130.1| (AJ248286) DNA REPAIR PROTEIN RAD32 (RAD32) [Pyrococcus abyssi] Length = 423 Score = 873 bits (2230), Expect = 0.0 Identities = 423/423 (100%), Positives = 423/423 (100%) Query: 1 MWTSEVFVMGIKFAHLADVHLGYEQFNRSQRAEEFAKAFEDAIKICVDEKVDFIVIAGDL 60 MWTSEVFVMGIKFAHLADVHLGYEQFNRSQRAEEFAKAFEDAIKICVDEKVDFIVIAGDL Sbjct: 1 MWTSEVFVMGIKFAHLADVHLGYEQFNRSQRAEEFAKAFEDAIKICVDEKVDFIVIAGDL 60 Query: 61 FNSSRPSPGTIKTAVKILQIPRDNNIPVFAIEGNHDRTQRGPSILHLLEDLGLLYVLGVR 120 FNSSRPSPGTIKTAVKILQIPRDNNIPVFAIEGNHDRTQRGPSILHLLEDLGLLYVLGVR Sbjct: 61 FNSSRPSPGTIKTAVKILQIPRDNNIPVFAIEGNHDRTQRGPSILHLLEDLGLLYVLGVR 120 Query: 121 DEKVENEYLTSEKTKAGWLVKGMYKDVEIHGMKYMSAAWFEGNIELFKSMFRPEGDAILV 180 DEKVENEYLTSEKTKAGWLVKGMYKDVEIHGMKYMSAAWFEGNIELFKSMFRPEGDAILV Sbjct: 121 DEKVENEYLTSEKTKAGWLVKGMYKDVEIHGMKYMSAAWFEGNIELFKSMFRPEGDAILV 180 Query: 181 LHQGVREITENNYPNYSSELSLSDLPKGYLYYALGHIHKRFELTYDDAPVVYPGSLERWD 240 LHQGVREITENNYPNYSSELSLSDLPKGYLYYALGHIHKRFELTYDDAPVVYPGSLERWD Sbjct: 181 LHQGVREITENNYPNYSSELSLSDLPKGYLYYALGHIHKRFELTYDDAPVVYPGSLERWD 240 Query: 241 FGDYSLKLTWNGFQFKEEVGVDKGFYIVEDFKPRFIRINARDFIDVHIKGHSENEIKKAV 300 FGDYSLKLTWNGFQFKEEVGVDKGFYIVEDFKPRFIRINARDFIDVHIKGHSENEIKKAV Sbjct: 241 FGDYSLKLTWNGFQFKEEVGVDKGFYIVEDFKPRFIRINARDFIDVHIKGHSENEIKKAV 300 Query: 301 KLAVPKIPRNSYVRFNIRWKRPVDVDWIKSIVNAEYVRVNPIIIKEERGPDGKDLDVKKF 360 KLAVPKIPRNSYVRFNIRWKRPVDVDWIKSIVNAEYVRVNPIIIKEERGPDGKDLDVKKF Sbjct: 301 KLAVPKIPRNSYVRFNIRWKRPVDVDWIKSIVNAEYVRVNPIIIKEERGPDGKDLDVKKF 360 Query: 361 FTELEWKIIELASEKEYEYYLNQIIDLLASEEGKVEAKIDAKREEKKFVRPKNPGDIMAW 420 FTELEWKIIELASEKEYEYYLNQIIDLLASEEGKVEAKIDAKREEKKFVRPKNPGDIMAW Sbjct: 361 FTELEWKIIELASEKEYEYYLNQIIDLLASEEGKVEAKIDAKREEKKFVRPKNPGDIMAW 420 Query: 421 VKG 423 VKG Sbjct: 421 VKG 423 >pir||D71083 probable phosphoesterase (EC 3.1.-.-) PHAR032 - Pyrococcus horikoshii >gi|3257343|dbj|BAA30026.1| (AP000004) 413aa long hypothetical protein [Pyrococcus horikoshii] Length = 413 Score = 628 bits (1603), Expect = e-179 Identities = 289/415 (69%), Positives = 352/415 (84%), Gaps = 5/415 (1%) Query: 11 IKFAHLADVHLGYEQFNRSQRAEEFAKAFEDAIKICVDEKVDFIVIAGDLFNSSRPSPGT 70 +KFAHLADVHLGYEQFN+ QRAEEFA F+ A+++CV E VDFI+IAGDLFNSSRPSPGT Sbjct: 1 MKFAHLADVHLGYEQFNKPQRAEEFANTFKKALEMCVKESVDFIIIAGDLFNSSRPSPGT 60 Query: 71 IKTAVKILQIPRDNNIPVFAIEGNHDRTQRGPSILHLLEDLGLLYVLGVRDEKVENEYLT 130 IKTA+K+LQIP++NNIPVFAIEGNHDRTQRGPS+LHLLEDLGLLYV+G+R E+VENEYLT Sbjct: 61 IKTAIKLLQIPKENNIPVFAIEGNHDRTQRGPSVLHLLEDLGLLYVIGLRQERVENEYLT 120 Query: 131 SEKTKAGWLVKGMYKDVEIHGMKYMSAAWFEGNIELFKSMFRPEGDAILVLHQGVREITE 190 SE+ WLVKG+Y ++EIHGMKYMS+AWFE N+ FK +FRP+ DAILVLHQG+R+ITE Sbjct: 121 SERVGNYWLVKGVYDNLEIHGMKYMSSAWFEANLNFFKGIFRPDEDAILVLHQGIRDITE 180 Query: 191 NNYPNYSSELSLSDLPKGYLYYALGHIHKRFELTYDDAPVVYPGSLERWDFGDYSLKLTW 250 +P+YS+EL LSDLP+GYLYYALGH+HKRFE Y D+PVVYPGSLERWDFGDY+ +L W Sbjct: 181 KVFPSYSAELKLSDLPRGYLYYALGHVHKRFETNYGDSPVVYPGSLERWDFGDYAKRLVW 240 Query: 251 NGFQFKEEVGVDKGFYIVEDFKPRFIRINARDFIDVHIKGHSENEIKKAVKLAVPKIPRN 310 NG F+EEVG DKGFYIVEDF PRF+ I RDFIDV IKG SE EIKKAVK ++P IPRN Sbjct: 241 NGVTFREEVGSDKGFYIVEDFTPRFVNIKVRDFIDVVIKGDSEREIKKAVKASLPHIPRN 300 Query: 311 SYVRFNIRWKRPVDVDWIKSIVNAEYVRVNPIIIKEERGPDGKDLDVKKFFTELEWKIIE 370 SYVRFNI+W++P DV+WIK IVNAEY+R++ IIK+E+ +G+ +D+K FFTE EWK+I+ Sbjct: 301 SYVRFNIKWRKPFDVEWIKEIVNAEYLRIHTAIIKDEKSMNGESVDIKSFFTEQEWKVID 360 Query: 371 LASEKEYEYYLNQIIDLLASEEGKVEAKIDAKREEK--KFVRPKNPGDIMAWVKG 423 LAS E+E Y+ +I+D+L+ E K E + K+E K KF RPKNPGD+ AW++G Sbjct: 361 LASSDEFESYIEKIVDILSGYEKKEERE---KKESKIRKFERPKNPGDLTAWLRG 412 >gi|11498636 DNA repair protein RAD32 (rad32) [Archaeoglobus fulgidus] >gi|7428119|pir||G69378 probable phosphoesterase (EC 3.1.-.-) AF1031 - Archaeoglobus fulgidus >gi|2649563|gb|AAB90212.1| (AE001032) DNA repair protein RAD32 (rad32) [Archaeoglobus fulgidus] Length = 443 Score = 246 bits (622), Expect = 3e-64 Identities = 157/411 (38%), Positives = 237/411 (57%), Gaps = 28/411 (6%) Query: 11 IKFAHLADVHLGYEQFNRSQRAEEFAKAFEDAIKICVDEKVDFIVIAGDLFNSSRPSPGT 70 +KFAH+ADVHLGYEQ+N+ RAE+FAKAF+ + V+ DF+VIAGDLF+ S PSP T Sbjct: 1 MKFAHIADVHLGYEQYNQPWRAEDFAKAFKVIAEKAVESNADFVVIAGDLFHRSLPSPRT 60 Query: 71 IKTAVKILQIPRDNNIPVFAIEGNHDRTQRGPSILHLLEDLGLLYVLGVRDEKVENEYLT 130 IK AV+ L + R NIPVFA+EGNHD+T R S HLLE LGLL VLG+R V E + Sbjct: 61 IKEAVETLWMFRKENIPVFAVEGNHDKTSRDISAYHLLESLGLLNVLGLRRNPVRGENVE 120 Query: 131 SEKTKAGWLVKGMYKDVEIHGMKYMSAAWFEGNIELFKSMFRPEGD-AILVLHQGVREIT 189 S + + +LVKG+ DVEI G ++ S + +E + +P+ D ++LVLHQ V+E+ Sbjct: 121 SLRIQNVYLVKGVVDDVEILGDRHRS----KWQLEKVLPLLKPQSDKSVLVLHQAVKEVV 176 Query: 190 ENNYPNYSSELSLSDLPKGYLYYALGHIHKRFELTYDDAPVVYPGSLERWDFGDYSLKLT 249 + + + + EL+++DLP+ YYA GHIH +D + YPGS+ER+D + S K+ Sbjct: 177 DIDL-DMAYELTINDLPEA-SYYAFGHIHLPKIYEFDGKAIAYPGSVERYDLREAS-KIV 233 Query: 250 WNGFQFKEEVGVDKGFYIVEDFKPRFIRINARDFIDVHIKGHSENEIKKAVKLAVPKIPR 309 + + G+ KGF +V++F+P F+ I R+ DV I+ S ++K + + + Sbjct: 234 RYRDELVLKDGIRKGFILVKNFRPEFVEIETRELYDVEIEDESVEGLEKKFLEVLGRADK 293 Query: 310 NSYVRFNIRWKRPVDVDWIKSIV--NAEYVRVNPIIIKEERGPDGKDLDVK---KFFTEL 364 + ++ VDV + + Y + I EE +++++K +FFTE Sbjct: 294 EGIMVAKLKSSDAVDVRRLSEVAAKRVRYAEIRFERILEEI----EEVEIKQESEFFTEF 349 Query: 365 EWKIIEL----ASEKE-----YEYYLNQIIDLLASEEGKVEAKIDAKREEK 406 E K++EL E E E+YL+ L EEG E ++ + E+K Sbjct: 350 ELKLLELLRGEMDEDEVYSLVVEHYLSG--GALKQEEGAEERVVEEETEKK 398 >gb|AAK42418.1| DNA repair protein (rad32/mre11) [Sulfolobus solfataricus] Length = 381 Score = 118 bits (293), Expect = 1e-25 Identities = 69/223 (30%), Positives = 118/223 (51%), Gaps = 24/223 (10%) Query: 11 IKFAHLADVHLGYEQFNRSQRAEEFAKAFEDAIKICVDEKVDFIVIAGDLFNSSRPSPGT 70 ++ H++D HLG Q++ +R ++ F + I + E VD I+ +GDLF+ S P+ Sbjct: 2 VQILHISDTHLGKRQYSLVEREKDIYDIFSQLVDIAIKEHVDVIIHSGDLFDVSSPTTNA 61 Query: 71 IKTAVKILQIPRDNNIPVFAIEGNHDRTQRGPSIL--HLLEDLGLLYVLGVRDEKVENEY 128 + A+KIL+ +D NIP +I G+HD +R ++ ++L +L L+ +L E Y Sbjct: 62 LVMAIKILKRLKDVNIPFLSIPGDHDTPKRKGYLIPHNILSELDLIKIL-----NYEKPY 116 Query: 129 LTSEKTKAGWLVKGMYKDVEIHGMKYMSAAWFEGNIELFKSMFRPEGDAILVLHQGVREI 188 + K +E++G+ ++ + ++ +IL+LHQGV++I Sbjct: 117 I--------------IKGIEVYGIPHIPTVSKSILVSALSALRPKSSRSILLLHQGVKQI 162 Query: 189 TENNYPNYSSELSLSDLPKGYLYYALGHIHKRFELTYDDAPVV 231 + S ++ L LPKG+ YYALGHIH R+ LT DD V+ Sbjct: 163 LPY---DGSWQMELGSLPKGFGYYALGHIHTRWRLTQDDGSVI 202 >pir||B64465 probable phosphoesterase (EC 3.1.-.-) MJ1323 - Methanococcus jannaschii >gi|1591963|gb|AAB99332.1| (U67572) DNA repair protein RAD32 (rad32) [Methanococcus jannaschii] Length = 366 Score = 116 bits (287), Expect = 6e-25 Identities = 101/305 (33%), Positives = 148/305 (48%), Gaps = 46/305 (15%) Query: 13 FAHLADVHLGYEQFNRSQRAEEFAKAFEDAIKICVDEKVDFIVIAGDLFNSSRPSPGTIK 72 F H+AD HLGY Q+N R ++ +F+ IK ++ K D ++ +GDLFN RP ++ Sbjct: 3 FVHIADNHLGYRQYNLDDREKDIYDSFKLCIKKILEIKPDVVLHSGDLFNDLRPPVKALR 62 Query: 73 TAVKILQIPRDNNIPVFAIEGNHDRTQR--GPSILHLLEDLGLLYVLGVRDEKVENEYLT 130 A++ + +NNI V+ + GNH+ +R S L LL+D YV + + V N Sbjct: 63 IAMQAFKKLHENNIKVYIVAGNHEMPRRLGEESPLALLKD----YVKILDGKDVIN---- 114 Query: 131 SEKTKAGWLVKGMYKDVEIHGMKYMSAAWFE---GNIELFKSMFRPEGDAILVLHQGVRE 187 V G +++ I G Y + E ++ F+S + IL+LHQG+ Sbjct: 115 ---------VNG--EEIFICGTYYHKKSKREEMLDKLKNFESEAKNYKKKILMLHQGI-- 161 Query: 188 ITENNYPNYSSELSLSDLPKGYLYYALGHIHKRFELTYDDAPVVYPGSLE---RWDFGDY 244 N Y EL DLPK + YYALGHIHKR ++D + Y GS E R ++ DY Sbjct: 162 ---NPYIPLDYELEHFDLPK-FSYYALGHIHKRILERFNDGILAYSGSTEIIYRNEYEDY 217 Query: 245 SLKLTWNGFQFKEEVGVDKGFYIVEDFKPRFIRINARDFIDVHIKGHSENEIKKAVKLAV 304 K GF + G D +E I I R+F++V+IK KK+ AV Sbjct: 218 --KKEGKGFYLVDFSGNDLDISDIEK-----IDIECREFVEVNIKD------KKSFNEAV 264 Query: 305 PKIPR 309 KI R Sbjct: 265 NKIER 269 >pir||E69171 conserved hypothetical protein MTH541 - Methanobacterium thermoautotrophicum (strain Delta H) >gi|2621616|gb|AAB85047.1| (AE000837) Rad32 related protein [Methanobacterium thermoautotrophicum] Length = 587 Score = 104 bits (258), Expect = 2e-21 Identities = 85/297 (28%), Positives = 147/297 (48%), Gaps = 39/297 (13%) Query: 12 KFAHLADVHLGYEQFN--RSQRAEEFAKAFEDAIKICVDEKVDFIVIAGDLFNSSRPSPG 69 +FAHL+D HLG ++ R E F A +DA++ + VDF++IAGDLF+S+ P+ Sbjct: 172 RFAHLSDCHLGAQKHPDLRELEFEAFRMALDDALQ----KDVDFMIIAGDLFHSNIPNME 227 Query: 70 TIKTAVKILQIPRDNNIPVFAIEGNHDRTQRGPSILHLLEDLGLL--YVLGVRDEKVENE 127 T+K A L+ R+ +P++ G+HD + S++ +LE G++ V + +K+ E Sbjct: 228 TVKRATLELRRVREAGVPIYVNYGSHDYSPSSTSMIDILESAGVIDKVVRPIPGKKLGLE 287 Query: 128 YLTSEKTKAGWLVKGMYKDVEIHGMKYMSAAWFEGNIELFKSMFRPEGDAILVLHQGVRE 187 + EKT A K + G A + ++ +++ +G I + H + + Sbjct: 288 FTVDEKTGA--------KITGLSGRSRTLEAEYFMKLDR-EALEAEDGFRIFLFHSAITQ 338 Query: 188 ITENNYPNYSSELSLSDLPKGYLYYALGHIHKR---FELTYDDAPVVYPGSLERWDFGDY 244 + + S + L+ P+G+ YYA GH+H++ E Y P+VYPG+L FG Y Sbjct: 339 FKPVDLADMES-VDLNLFPRGFEYYAGGHVHRKGCYIEEGY--GPIVYPGTL----FGSY 391 Query: 245 SLKLTWNGFQFKEEVGVDKGFYIVE----DFKPRFIRINARDF--IDVHIKGHSENE 295 + L N G +G+Y+VE +P F I +F I+ + G + + Sbjct: 392 AGDLEENA------RGETRGYYLVEFTDRAREPEFREIRPAEFEYIECDVTGKNSQD 442 >emb|CAC11305.1| (AL445063) conserved hypothetical protein [Thermoplasma acidophilum] Length = 376 Score = 80.4 bits (195), Expect = 4e-14 Identities = 60/223 (26%), Positives = 97/223 (42%), Gaps = 15/223 (6%) Query: 15 HLADVHLGYEQFNRSQRAEEFAKAFEDAIKICVDEKVDFIVIAGDLFNSSRPSPGTIKTA 74 H +D H+GY +R ++F AF +AI I ++ VDF++ GDLF++ P I+ Sbjct: 2 HFSDTHIGYRSLTLDEREQDFYDAFHEAIDIALENSVDFVIHTGDLFDTWVPGNRAIREF 61 Query: 75 VKILQIPRDNNIPVFAIEGNHDRTQRGPSILHLLEDLGLLYVLGVRDEKVENEYLTSEKT 134 + NIP+F I G+HDR +R + D ++VLG EY + Sbjct: 62 RNAVMKLNARNIPIFVIFGDHDRPRRNGEPAAGIFDFMGVHVLGWD----AYEYAVRKFD 117 Query: 135 KAGWLVKGMYKDVEIHGMKYMSAAWFEGNIELFKSMFRPEGDAILVLHQGVREITENNYP 194 L+ G I MK + + +++ + IL+ HQ + + + Sbjct: 118 GEDVLIGG------ISNMKGYMKTRLKDEYKRSENIEEGYRNRILMSHQAI----DPFFV 167 Query: 195 NYSSELSLSDLPKGYLYYALGHIHKRFELTYDDAPVVYPGSLE 237 E + DLP + Y A+GH+H E + Y GS E Sbjct: 168 PDQCEAKMDDLPMNFSYIAMGHLHDFMERRIGPL-ISYAGSTE 209 >pir||E70462 ATP-dependent dsDNA exonuclease (EC 3.1.-.-) - Aquifex aeolicus >gi|2984155|gb|AAC07689.1| (AE000761) ATP-dependent dsDNA exonuclease [Aquifex aeolicus] Length = 379 Score = 78.8 bits (191), Expect = 1e-13 Identities = 68/258 (26%), Positives = 113/258 (43%), Gaps = 19/258 (7%) Query: 11 IKFAHLADVHLGYEQFNRSQRAEEFAKAFEDAIKICVDEKVDFIVIAGDLFNSSRPSPGT 70 ++ HL+D+H G + R R E+ A + C + K D +++AGD+F+ + P Sbjct: 1 MRLIHLSDIHAG-KNLGRVSRNEDVVYALNQVVDFCKENKPDLVLVAGDVFDKANPDNEA 59 Query: 71 IKTAVKILQIPRDNNIPVFAIEGNHDRTQRGPSILHLLEDLGLLYV---------LGVRD 121 + NIP I GNHD + S+ LL+ + + V L V+ Sbjct: 60 KEIIFDFFLKLHSLNIPSVVISGNHDSYEFMKSLKKLLQVIDVYVVPKPNLEECVLDVKG 119 Query: 122 EKVENEYLTSEK--TKAGWLVKGMYKDVEIHGMKYMSAAWFEGNIELFKSMFRPEGDAIL 179 K+ SE+ T+AG K Y + +KY+ + +++ S G Sbjct: 120 LKIACLPYPSERVLTRAGEDSKLSYAVLVEKAIKYLYEKVKDAPLKVLLSHLFIAGSKYT 179 Query: 180 VLHQGVREITENNYPNYSSELSLSDLPKGYLYYALGHIHKRFELTYDDAPVVYPGSLERW 239 + RE T +Y Y+ E S +P+G+ Y ALGH+H+ + V Y GSL + Sbjct: 180 ---RTEREATITDY--YAVEPSA--IPEGFDYVALGHVHRYQRIEKAPTHVYYTGSLFQL 232 Query: 240 DFGDYSLKLTWNGFQFKE 257 DF + + +N + KE Sbjct: 233 DFSEAGQEKFFNFVELKE 250 >gb|AAG19044.1| (AE005003) phosphoesterase; Ppe [Halobacterium sp. NRC-1] Length = 387 Score = 76.9 bits (186), Expect = 4e-13 Identities = 40/99 (40%), Positives = 58/99 (58%), Gaps = 1/99 (1%) Query: 15 HLADVHLGYEQFNRSQRAEEFAKAFEDAIKICVDEKVDFIVIAGDLFNSSRPSPGTIKTA 74 H D HLGY+Q++ QR ++F AF+ I +DE VD +V AGDL++ +P I Sbjct: 6 HTGDTHLGYQQYHAPQRRQDFLDAFDAVITDAIDEGVDAVVHAGDLYHDRQPGLRDILDT 65 Query: 75 VKILQIPRDNNIPVFAIEGNHDRTQRGPSILHLLEDLGL 113 + +L+ +D +IP A+ GNH+ T R L L E LGL Sbjct: 66 IALLRPLQDADIPFLAVVGNHEGT-RDAQWLDLFETLGL 103 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.320 0.140 0.420 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 169020784 Number of Sequences: 2977 Number of extensions: 7578935 Number of successful extensions: 16159 Number of sequences better than 1.0e-10: 9 Number of HSP's better than 0.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 16138 Number of HSP's gapped (non-prelim): 10 length of query: 423 length of database: 189,106,746 effective HSP length: 54 effective length of query: 369 effective length of database: 156,788,448 effective search space: 57854937312 effective search space used: 57854937312 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.7 bits) S2: 166 (69.1 bits)