BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0890 (leuA-1) DE:2-isopropylmalate synthase (leuA-1)
(486 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||A75045 2-isopropylmalate synthase (leua-1) PAB0890 - Pyroco... 951 0.0
sp|O67862|LEU1_AQUAE 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 549 e-155
dbj|BAB06777.1| (AP001517) 2-isopropylmalate synthase [Bacillus ... 528 e-149
sp|Q9WZ23|LEU1_THEMA 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 506 e-142
sp|P48575|LEU1_ANASP 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 499 e-140
sp|P94907|LEU1_MICAE 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 495 e-139
sp|P94565|LEU1_BACSU 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 489 e-137
sp|P48576|LEU1_SYNY3 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 488 e-137
pir||G81125 2-isopropylmalate synthase NMB1070 [imported] - Neis... 478 e-134
pir||C81895 probable 2-isopropylmalate synthase (EC 4.1.3.12) NM... 477 e-133
pir||F75391 2-isopropylmalate synthase - Deinococcus radiodurans... 476 e-133
sp|Q02141|LEU1_LACLA 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 476 e-133
gb|AAB81913.1| (U92974) LeuA [Lactococcus lactis] 476 e-133
pir||F82070 2-isopropylmalate synthase VC2490 [imported] - Vibri... 447 e-124
sp|Q58595|YB95_METJA PUTATIVE 2-ISOPROPYLMALATE/HOMOCITRATE SYNT... 445 e-124
pir||E64106 2-isopropylmalate synthase (EC 4.1.3.12) - Haemophil... 442 e-123
sp|P43861|LEU1_HAEIN 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 442 e-123
pir||C81270 2-isopropylmalate synthase (EC 4.1.3.12) Cj1719c [im... 442 e-123
gi|10957107 2-isopropylmalate synthase [Buchnera sp. APS] >gi|11... 441 e-123
emb|CAA07305.2| (AJ006878) 2-isoproylmalate synthase [Buchnera a... 441 e-123
pir||A82634 2-isopropylmalate synthase XF1818 [imported] - Xylel... 436 e-121
sp|P09151|LEU1_ECOLI 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 436 e-121
pir||S40588 hypothetical protein - Escherichia coli >gi|216492|d... 434 e-120
gi|10954458 2-isopropylmalate synthase [Buchnera aphidicola] >gi... 434 e-120
sp|P48571|LEU1_BUCRP 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 433 e-120
gi|10954450 isopropylmalate synthase [Buchnera aphidicola] >gi|3... 431 e-120
gb|AAG31401.1| (AF197456) 2-isopropylmaltate synthase [Buchnera ... 427 e-118
sp|O04973|LU1A_LYCPN 2-ISOPROPYLMALATE SYNTHASE A (ALPHA-ISOPROP... 425 e-118
gb|AAG31404.1| (AF197457) 2-isopropylmaltate synthase [Buchnera ... 424 e-117
gb|AAF36732.1| (AC016662) putative 2-isopropylmalate synthase; 3... 423 e-117
gb|AAG31392.1| (AF197453) 2-isopropylmaltate synthase [Buchnera ... 418 e-116
gb|AAG31395.1| (AF197454) 2-isopropylmaltate synthase [Buchnera ... 417 e-115
gb|AAG31398.1| (AF197455) 2-isopropylmaltate synthase [Buchnera ... 414 e-115
gb|AAG31389.1| (AF197452) 2-isopropylmaltate synthase [Buchnera ... 413 e-114
gb|AAG31377.1| (AF197448) 2-isopropylmaltate synthase [Buchnera ... 411 e-114
gb|AAG31927.1|AF200469_1 (AF200469) 2-isopropylmalate synthase [... 411 e-114
gb|AAG31386.1| (AF197451) 2-isopropylmaltate synthase [Buchnera ... 409 e-113
sp|O04974|LU1B_LYCPN 2-ISOPROPYLMALATE SYNTHASE B (ALPHA-ISOPROP... 408 e-113
gb|AAG31383.1| (AF197450) 2-isopropylmaltate synthase [Buchnera ... 404 e-111
pir||D69064 isopropylmalate synthase - Methanobacterium thermoau... 404 e-111
gb|AAF26002.1|AC013354_21 (AC013354) F15H18.3 [Arabidopsis thali... 399 e-110
gi|11497835 2-isopropylmalate synthase (leuA-2) [Archaeoglobus f... 396 e-109
sp|Q39891|LEU1_SOYBN PROBABLE 2-ISOPROPYLMALATE SYNTHASE (ALPHA-... 393 e-108
pir||F69196 2-isopropylmalate synthase - Methanobacterium thermo... 390 e-107
sp|Q58787|YD92_METJA PUTATIVE 2-ISOPROPYLMALATE/HOMOCITRATE SYNT... 388 e-106
gb|AAG31926.1|AF200468_1 (AF200468) 2-isopropylmalate synthase [... 385 e-106
gb|AAG31380.1| (AF197449) 2-isopropylmaltate synthase [Buchnera ... 383 e-105
dbj|BAB08874.1| (AB026660) 2-isopropylmalate synthase-like; homo... 372 e-102
gi|11498562 2-isopropylmalate synthase (leuA-1) [Archaeoglobus f... 367 e-100
dbj|BAB09819.1| (AB006708) 2-isopropylmalate synthase-like prote... 359 3e-98
sp|P15875|LEU1_SALTY 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 347 1e-94
sp|Q57926|Y503_METJA PUTATIVE 2-ISOPROPYLMALATE/HOMOCITRATE SYNT... 315 6e-85
pir||H69084 2-isopropylmalate synthase - Methanobacterium thermo... 296 5e-79
emb|CAA72697.1| (Y11966) 2-isopropylmalate synthase [Buchnera ap... 286 4e-76
sp|Q56216|LEU1_THETH 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 280 3e-74
gb|AAK42554.1| 2-isopropylmalate synthase, putative (leuA-3) [S... 279 5e-74
gb|AAK41252.1| 2-isopropylmalate synthase (leuA-2) [Sulfolobus s... 249 5e-65
gb|AAF13763.1|AF117351_20 (AF117351) homocitrate synthase [Zymom... 233 3e-60
sp|O66682|LY41_AQUAE PUTATIVE LYASE AQ_356 >gi|7447064|pir||F703... 225 8e-58
gb|AAA22169.1| (M17349) nifV protein [Azotobacter vinelandii] 222 7e-57
sp|P05342|NIFV_AZOVI HOMOCITRATE SYNTHASE >gi|420863|pir||S29758... 222 9e-57
emb|CAB91069.1| (AJ239033) homocitrate sythase 1 [Anabaena varia... 215 1e-54
gb|AAG27075.1|AF105225_11 (AF105225) NifV [Acetobacter diazotrop... 212 7e-54
emb|CAB91068.1| (AJ239032) homocitrate synthase 2 [Anabaena vari... 208 1e-52
sp|P23122|NIFV_AZOCH HOMOCITRATE SYNTHASE >gi|280010|pir||C43706... 205 9e-52
pir||E75045 2-isopropylmalate synthase (leua-3) PAB0894 - Pyroco... 204 2e-51
pir||B71181 probable 2-isopropylmalate synthase - Pyrococcus hor... 203 5e-51
pir||G75159 2-isopropylmalate synthase (leua-2) PAB0286 - Pyroco... 200 2e-50
sp|Q01181|NIFV_RHOSH HOMOCITRATE SYNTHASE >gi|282178|pir||D41880... 200 3e-50
gb|AAK40481.1| 2-isopropylmalate synthase, putative (leuA-1) [S... 197 2e-49
sp|Q47884|NIFV_FRAAL HOMOCITRATE SYNTHASE >gi|1256029|gb|AAA9626... 195 8e-49
sp|P54610|NIFV_FRASP HOMOCITRATE SYNTHASE >gi|1280032|gb|AAB3687... 194 2e-48
sp|Q07179|NIFV_RHOCA HOMOCITRATE SYNTHASE >gi|486695|pir||S34817... 188 1e-46
sp|Q9WZ22|LY41_THEMA PUTATIVE LYASE TM0552 >gi|7447065|pir||F723... 182 8e-45
gi|6320019 homocitrate synthase, highly homologous to YDL131W; L... 181 2e-44
gi|6320071 homocitrate synthase, highly homologous to YDL182W; L... 181 2e-44
sp|O87198|HOSC_THETH HOMOCITRATE SYNTHASE >gi|11268243|pir||T511... 181 2e-44
sp|O94225|HOSM_PENCH HOMOCITRATE SYNTHASE, MITOCHONDRIAL PRECURS... 177 2e-43
sp|P74269|LY41_SYNY3 PUTATIVE LYASE SLL1564 >gi|7447061|pir||S75... 176 4e-43
sp|Q12726|HOSM_YARLI HOMOCITRATE SYNTHASE, MITOCHONDRIAL PRECURS... 175 1e-42
pir||S02508 homocitrate synthase (EC 4.1.3.21) - Klebsiella pneu... 174 2e-42
sp|Q52070|NIFV_ENTAG HOMOCITRATE SYNTHASE >gi|1480128|emb|CAA680... 173 4e-42
pir||T39279 probable homocitrate synthase (EC 4.1.3.21), mitocho... 173 4e-42
sp|P05345|NIFV_KLEPN HOMOCITRATE SYNTHASE >gi|479601|pir||S34848... 168 2e-40
pir||S37297 nifV protein - Klebsiella pneumoniae >gi|149345|gb|A... 166 4e-40
sp|O31046|LEU1_STRCO 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 165 9e-40
sp|O86511|LY41_STRCO PUTATIVE LYASE SC1C2.10 >gi|7480336|pir||T2... 160 3e-38
sp|Q44290|NIFV_ANASP HOMOCITRATE SYNTHASE >gi|1487879|emb|CAA681... 154 2e-36
pir||D75418 homocitrate synthase - Deinococcus radiodurans (stra... 154 2e-36
emb|CAB69749.1| (AL137166) 2-ispoprylmalate synthase [Streptomyc... 152 8e-36
gi|6324225 alpha-isopropylmalate synthase (2-Isopropylmalate Syn... 148 1e-34
gb|AAA34744.1| (M12893) alpha-isopropylmalate synthase (EC 4.1.3... 148 1e-34
sp|O59736|LEU1_SCHPO 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 147 3e-34
sp|Q00853|NIVA_CLOPA HOMOCITRATE SYNTHASE, ALPHA SUBUNIT >gi|986... 147 3e-34
sp|Q9X7L2|LEU1_RHIME 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 147 3e-34
emb|CAB39977.1| (AJ132004) 2-isopropylmalate synthase [Sinorhizo... 146 4e-34
gi|6324682 Yor108wp [Saccharomyces cerevisiae] >gi|2132057|pir||... 146 6e-34
pir||I40725 2-isopropylmalate synthase (EC 4.1.3.12) - Corynebac... 145 1e-33
sp|P42455|LEU1_CORGL 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 145 1e-33
pir||C83494 probable 2-isopropylmalate synthase PA1217 [imported... 145 1e-33
gb|AAF66618.1|AF142777_1 (AF142777) homocitrate synthase [Neuros... 142 7e-33
pir||C83170 2-isopropylmalate synthase PA3792 [imported] - Pseud... 139 5e-32
sp|P70728|NIFV_AZOBR HOMOCITRATE SYNTHASE >gi|1597740|gb|AAC4617... 132 6e-30
emb|CAA91564.1| (Z67750) putative protein [Saccharomyces cerevis... 122 9e-27
sp|P96420|LEU1_MYCTU 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYL... 120 5e-26
emb|CAB60077.1| (Y18874) 2-isopropylmalate synthase [Synechococc... 104 2e-21
pir||F57256 2-isopropylmalate synthase homolog - Haemophilus inf... 83 6e-15
gb|AAA70115.1| (U18657) LeuA [Haemophilus influenzae] 81 2e-14
emb|CAA19166.1| (AL023596) 2-isopropylmalate synthase [Mycobacte... 80 4e-14
dbj|BAA90528.1| (AB029822) 2-phosphinomethylmalic acid synthase ... 79 1e-13
pir||B70567 probable mhpE protein - Mycobacterium tuberculosis (... 75 2e-12
gb|AAD02153.1| (AF039534) 2-oxo-4-hydroxypentanoate aldolase [Ps... 73 5e-12
sp|Q00852|NIVO_CLOPA HOMOCITRATE SYNTHASE, OMEGA SUBUNIT >gi|986... 73 9e-12
sp|P51016|DMPG_PSESP 4-HYDROXY-2-OXOVALERATE ALDOLASE (HOA) >gi|... 72 2e-11
dbj|BAB04853.1| (AP001511) hydroxymethylglutaryl-CoA lyase [Baci... 71 3e-11
pir||D69893 hydroxymethylglutaryl-CoA lyase homolog yngG - Bacil... 70 6e-11
>pir||A75045 2-isopropylmalate synthase (leua-1) PAB0890 - Pyrococcus abyssi
(strain Orsay) >gi|5458767|emb|CAB50254.1| (AJ248287)
2-isopropylmalate synthase (leuA-1) [Pyrococcus abyssi]
Length = 486
Score = 951 bits (2431), Expect = 0.0
Identities = 486/486 (100%), Positives = 486/486 (100%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI
Sbjct: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL 120
ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL
Sbjct: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL 120
Query: 121 AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY 180
AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY
Sbjct: 121 AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY 180
Query: 181 ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL 240
ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL
Sbjct: 181 ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL 240
Query: 241 EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ 300
EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ
Sbjct: 241 EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ 300
Query: 301 DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFK 360
DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFK
Sbjct: 301 DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFK 360
Query: 361 DIADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAW 420
DIADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAW
Sbjct: 361 DIADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAW 420
Query: 421 FKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTD 480
FKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTD
Sbjct: 421 FKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTD 480
Query: 481 IIEASA 486
IIEASA
Sbjct: 481 IIEASA 486
>sp|O67862|LEU1_AQUAE 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE) >gi|7447051|pir||B70479
2-isopropylmalate synthase (EC 4.1.3.12) - Aquifex
aeolicus >gi|2984301|gb|AAC07824.1| (AE000772)
2-isopropylmalate synthase [Aquifex aeolicus]
Length = 524
Score = 549 bits (1400), Expect = e-155
Identities = 285/488 (58%), Positives = 364/488 (74%), Gaps = 6/488 (1%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
KVYIFDTTLRDGEQ PG S+T EEK+++A QLAKLNVD+IEAGF AS+G+F+AV +IA
Sbjct: 8 KVYIFDTTLRDGEQAPGFSMTTEEKLQMAHQLAKLNVDIIEAGFAAASKGDFEAVNRIAK 67
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
EV+ P I +LARA+E DI+ A +AL AE+ RIHTFIATSPIHM+YKLR P++V +
Sbjct: 68 EVKGPVICSLARALESDIEIAAKALEPAERKRIHTFIATSPIHMEYKLRMTPDQVLERIK 127
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
KAV A +T+DVEFS EDATRS+ +FL E I GATVIN+PDTVGYA PEEF +L
Sbjct: 128 KAVSFARNFTDDVEFSCEDATRSEREFLYRAIETAIKHGATVINIPDTVGYAIPEEFGQL 187
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
+ + N+ NI V +SVHCHDDLGLA ANSL+A++ GA QVE T+NGIGERAGNAALEE
Sbjct: 188 IEDIMNNVPNIDKVILSVHCHDDLGLATANSLTAVKHGARQVECTINGIGERAGNAALEE 247
Query: 243 VIVALDVRRDFY-KVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQD 301
V++AL VR+DF+ + TDVN KEI +TS+L+ +TG V PNKAIVG NAFAHESGIHQ
Sbjct: 248 VVMALKVRKDFFGDLYTDVNTKEIYKTSRLLCRITGNFVQPNKAIVGDNAFAHESGIHQH 307
Query: 302 GVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKD 361
GVL R TYEI++P+ +GF S+IVLGKHSGRHA +++LEELG+ T+E L+R F+KFK+
Sbjct: 308 GVLSHRMTYEIMNPEDVGFPMSRIVLGKHSGRHALKRRLEELGFKFTKEELDRIFEKFKE 367
Query: 362 IADRKRWITDTDIEAIIQEELTKSNGKLKVEIIH--VTSG--KVSTATVRISMNGEERIE 417
+ADRK+ + D D+EA+I +E K V+++H V SG + TATV++ GEER E
Sbjct: 368 LADRKKEVYDEDLEALIYQEFMKIEDHEPVKVLHFQVQSGDNMIPTATVKLEFKGEER-E 426
Query: 418 VAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGL 477
+ NGP+DA AI KALG E KL +Y + ++T D+ E V +E++G GRG+
Sbjct: 427 ASSTGNGPVDATIKAIQKALGIEPKLLDYSIKALTPNTDAQAEARVVLELDGVKASGRGV 486
Query: 478 STDIIEAS 485
TDII+AS
Sbjct: 487 DTDIIKAS 494
>dbj|BAB06777.1| (AP001517) 2-isopropylmalate synthase [Bacillus halodurans]
Length = 515
Score = 528 bits (1346), Expect = e-149
Identities = 261/489 (53%), Positives = 370/489 (75%), Gaps = 3/489 (0%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
MRK+ +FDTTLRDGEQ+ GV+L EEK+EIAKQL +L VD+IEAGFP +S+G+F++VK I
Sbjct: 1 MRKISVFDTTLRDGEQSAGVNLNFEEKMEIAKQLERLGVDIIEAGFPASSQGDFQSVKAI 60
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL 120
A V+ ++ LAR+V+KDID A EAL++AE+ RIH FIATSPIHM++KLR PE+V +
Sbjct: 61 AETVKGSSVTGLARSVQKDIDAAWEALKSAEEPRIHLFIATSPIHMEHKLRMTPEQVIEK 120
Query: 121 AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY 180
AV++V++A + V++SAEDA+RSD+ FL ++ EAVI AGATVIN+PDTVGY TP+E
Sbjct: 121 AVESVKYAASRFKHVQWSAEDASRSDFPFLAQIIEAVIQAGATVINLPDTVGYTTPQEIK 180
Query: 181 ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL 240
+ +Y+++N+ +I V +S H HDDLG+AVANSL+AI+ GADQVE T+NGIGERAGNA+L
Sbjct: 181 RMFQYMKQNVPSIDKVSLSTHNHDDLGMAVANSLAAIQGGADQVECTINGIGERAGNASL 240
Query: 241 EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ 300
EE+ VAL++R+D Y+ +T + LKEI RTS LVS LTG+ VP NKA+VG NAFAHESGIHQ
Sbjct: 241 EEIAVALNIRKDHYETETGLILKEIKRTSSLVSKLTGMVVPNNKAVVGANAFAHESGIHQ 300
Query: 301 DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFK 360
DGVLK + TYEII P+ +G S + +VLGKHSGRHAF+ K++ELG++ T+E L FK FK
Sbjct: 301 DGVLKNKQTYEIITPEMVGVSSNSMVLGKHSGRHAFKTKVQELGFTGTDEQLNNIFKAFK 360
Query: 361 DIADRKRWITDTDIEAIIQEELT---KSNGKLKVEIIHVTSGKVSTATVRISMNGEERIE 417
D+AD+K+ IT+ D+ A++ E+ ++ +L+ ++ S +TAT+ + + E E
Sbjct: 361 DLADKKKEITEDDLFALMTEQTVGGETNHYELQTLQVNYGSNLTNTATITMKLPSGEVAE 420
Query: 418 VAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGL 477
A +G ++A+++ + + L KL +YR+ S+T G+D+L +V V+++ G + GRG
Sbjct: 421 EAATGSGSVEAIYNTLERLLDAPVKLLDYRIQSITGGRDALADVYVQMDYQGVVSSGRGT 480
Query: 478 STDIIEASA 486
+ D++EASA
Sbjct: 481 AHDVLEASA 489
>sp|Q9WZ23|LEU1_THEMA 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE) >gi|7447055|pir||G72362
2-isopropylmalate synthase - Thermotoga maritima (strain
MSB8) >gi|4981068|gb|AAD35638.1|AE001730_6 (AE001730)
2-isopropylmalate synthase [Thermotoga maritima]
Length = 513
Score = 506 bits (1288), Expect = e-142
Identities = 257/489 (52%), Positives = 351/489 (71%), Gaps = 3/489 (0%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
MR++ IFDTTLRDGEQ+PG S+++EEKVE+A L L VD+IEAGFP++S +F+AVK++
Sbjct: 1 MRRIKIFDTTLRDGEQSPGASMSIEEKVEMALMLEDLGVDLIEAGFPVSSPVQFEAVKRV 60
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNR--IHTFIATSPIHMKYKLRKEPEEVK 118
A+ V+ P + LAR VEKDID A EAL++ K++ IH FIATSPIH KYKLR E EE+
Sbjct: 61 ASAVQKPIVVGLARCVEKDIDAAYEALKDRPKDKRMIHVFIATSPIHRKYKLRMEKEEIL 120
Query: 119 KLAVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEE 178
+ + V +A ++ + VEFSAEDA+R++ FL+E Y+ I+AGAT INVPDTVGYA P+E
Sbjct: 121 ERIRRYVGYAKQFFDLVEFSAEDASRTEVPFLIEAYKTAIEAGATTINVPDTVGYALPDE 180
Query: 179 FYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNA 238
F EL++ LR + I+ V +SVHCH+DLGLAVANSL+A++ GA QVEVT+NGIGERAGN
Sbjct: 181 FGELIKTLREGVPGIENVDLSVHCHNDLGLAVANSLAAVQNGATQVEVTLNGIGERAGNC 240
Query: 239 ALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGI 298
ALEE ++ L VR+D +T + + I S+L++H+TG+ NK IVG N F HESGI
Sbjct: 241 ALEEFVMILKVRKDKLPYETGIKTELIYPASRLLTHITGLIPSRNKPIVGENVFLHESGI 300
Query: 299 HQDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKK 358
HQDGVLK R TYEI+ P +G S +VLG+HSG+HA RKKLE G L EE ++ F+K
Sbjct: 301 HQDGVLKHRETYEIMKPSDIGRSSETLVLGRHSGKHALRKKLETYGIKLDEETFQKVFEK 360
Query: 359 FKDIADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVTSGKVSTATVRISMN-GEERIE 417
F ++ADRK+ + D D+ +I+ E L + K+ HV +G T + + G E+ E
Sbjct: 361 FTELADRKKEVYDDDLFSIVSEVLREPINGYKLVHFHVHTGNTLLPTAAVVLQVGNEKRE 420
Query: 418 VAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGL 477
A NGP+DA+F AI+KALG + KL EY + +V +GK++ GEV + + ++ E++ GRG+
Sbjct: 421 AAEAGNGPVDAIFKAIDKALGIQPKLEEYIIQAVGTGKNAQGEVKLTLRIDNELYSGRGV 480
Query: 478 STDIIEASA 486
STDI+EASA
Sbjct: 481 STDIVEASA 489
>sp|P48575|LEU1_ANASP 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE) >gi|1084180|pir||S52294
2-isopropylmalate synthase (EC 4.1.3.12) - Anabaena sp.
(PCC 7120) >gi|662867|emb|CAA87005.1| (Z46907)
alpha-isopropylmalate synthase [Anabaena PCC7120]
Length = 531
Score = 499 bits (1270), Expect = e-140
Identities = 260/500 (52%), Positives = 354/500 (70%), Gaps = 16/500 (3%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
K+ IFDTTLRDGEQ PG +L ++EK+ IAKQLA+L VD+IEAGF AS G+F+AV KIA
Sbjct: 7 KIIIFDTTLRDGEQCPGATLNIDEKLAIAKQLARLGVDIIEAGFAFASPGDFEAVHKIAQ 66
Query: 63 EV---EDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKK 119
V P I +LARA DI A EA++ A K RIHTFIATS IH++YKL+K EV
Sbjct: 67 TVGTQSGPVICSLARARHDDIKAAAEAIKPAAKGRIHTFIATSDIHLQYKLKKTRPEVIA 126
Query: 120 LAVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEF 179
+A + V +A +T+DVEFS EDA RSD +FL +V E I AGAT IN+PDTVGY TP EF
Sbjct: 127 IAEEMVAYAKSFTDDVEFSPEDAGRSDPEFLYQVLERAIAAGATTINIPDTVGYTTPSEF 186
Query: 180 YELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAA 239
+++ ++ N+ NI ISVH H+DLGLAVAN L A++ GA Q+E T+NGIGERAGNAA
Sbjct: 187 GAIIKGIKENVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGERAGNAA 246
Query: 240 LEEVIVALDVRRDFY-----------KVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVG 288
LEE+++A+ VRR ++ + T+++ K+I +TS+LVS+LTG+ V PNKAIVG
Sbjct: 247 LEELVMAMHVRRQYFNPFLGRHPDSEEALTNIDTKQIYKTSRLVSNLTGMLVQPNKAIVG 306
Query: 289 GNAFAHESGIHQDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLT 348
NAFAHESGIHQDGVLK + TYEI+D + +G + ++IVLGKHSGR+AFR +L+ELG+ L+
Sbjct: 307 ANAFAHESGIHQDGVLKNKLTYEIMDAQLIGLTDNQIVLGKHSGRNAFRTRLKELGFELS 366
Query: 349 EEHLERAFKKFKDIADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVT--SGKVSTATV 406
E L +AF KFK++AD+K+ I+D D+EAI+ +E+ ++ +VE++ V+ + V ATV
Sbjct: 367 ETELNKAFVKFKEVADKKKEISDWDLEAIVNDEIQQAPDLFRVELVQVSAVAMHVQPATV 426
Query: 407 RISMNGEERIEVAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVE 466
+ E + A GP+DA++ AIN+ + +L E+ V SVT+G D++GEV +R+
Sbjct: 427 TLRTPDGEELTDAAIGTGPVDAVYKAINRVVNVPNQLIEFSVQSVTAGIDAIGEVTIRLR 486
Query: 467 VNGEIFVGRGLSTDIIEASA 486
+F G +TDII ASA
Sbjct: 487 YESRVFSGHAANTDIIVASA 506
>sp|P94907|LEU1_MICAE 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE) >gi|1783285|dbj|BAA12849.1|
(D85684) 2-isopropylmalate synthase [Microcystis
aeruginosa]
Length = 533
Score = 495 bits (1260), Expect = e-139
Identities = 264/501 (52%), Positives = 348/501 (68%), Gaps = 17/501 (3%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIA- 61
+V IFDTTLRDGEQ+PG +L V+EK+ IA+ LA+L VDVIEAGFP AS G+F+AV+KIA
Sbjct: 7 RVIIFDTTLRDGEQSPGAALNVDEKLTIARALARLGVDVIEAGFPHASPGDFEAVQKIAG 66
Query: 62 ---TEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVK 118
+E + P I LARA +KDI A +ALR A K RIHTF+ATS IH++YKL+K +EV
Sbjct: 67 SVGSEADSPIICGLARATQKDIKSAADALRPAAKPRIHTFLATSDIHLQYKLKKTRQEVL 126
Query: 119 KLAVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEE 178
++ + V +A + DVEFS EDA RSD +FL +V E I AGAT +N+PDTVGY TP E
Sbjct: 127 EIVPEMVAYAKSFLNDVEFSPEDAGRSDPEFLYQVLERAIAAGATTVNIPDTVGYTTPSE 186
Query: 179 FYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNA 238
F L+R ++ N+ NI ISVH HDDLGLAVAN L A++ GA Q+E T+NGIGERAGNA
Sbjct: 187 FGALIRGIKENVPNIDQAIISVHGHDDLGLAVANFLEAVKNGARQLECTINGIGERAGNA 246
Query: 239 ALEEVIVALDVRRDFY-----------KVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIV 287
+LEE+++AL VRR ++ + T +N KEI RTS+LVS+LTG+ V PNKAIV
Sbjct: 247 SLEELVMALHVRRSYFNPFLGRPAESTEPLTKINTKEIYRTSRLVSNLTGMIVQPNKAIV 306
Query: 288 GGNAFAHESGIHQDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSL 347
G NAFAHESGIHQDGVLK + TYEI+D + +G + ++IVLGK SGR+AFR +L+ELG+ L
Sbjct: 307 GANAFAHESGIHQDGVLKHKLTYEIMDAESIGLTNNQIVLGKLSGRNAFRSRLQELGFEL 366
Query: 348 TEEHLERAFKKFKDIADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVTSGKVS--TAT 405
+E L AF +FK++ADRK+ ITD D+EAI+ +E+ ++E++ V+ G + TAT
Sbjct: 367 SETELNNAFIQFKEMADRKKEITDRDLEAIVNDEIDTVPDHFRLELVQVSCGNNARPTAT 426
Query: 406 VRISMNGEERIEVAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRV 465
V I + A GP+DAL AI++ + +L + V VT G D+LGEV +R+
Sbjct: 427 VTIRTPDGSELSDAAIGTGPVDALCKAIDRVVQIPNELISFSVREVTEGIDALGEVTIRL 486
Query: 466 EVNGEIFVGRGLSTDIIEASA 486
G + R TDII ASA
Sbjct: 487 RYAGRTYSARAADTDIIVASA 507
>sp|P94565|LEU1_BACSU 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE) >gi|7447054|pir||H69649
2-isopropylmalate synthase (EC 4.1.3.12) - Bacillus
subtilis >gi|1770069|emb|CAA99531.1| (Z75208)
2-isopropylmalate synthase [Bacillus subtilis]
>gi|2635293|emb|CAB14788.1| (Z99118) 2-isopropylmalate
synthase [Bacillus subtilis]
Length = 518
Score = 489 bits (1246), Expect = e-137
Identities = 246/491 (50%), Positives = 350/491 (71%), Gaps = 6/491 (1%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
MRK+ FDTTLRDGEQ+PGV+L +EK+ IAKQL +L D+IEAGFP +S G+F AV++I
Sbjct: 1 MRKINFFDTTLRDGEQSPGVNLNTQEKLAIAKQLERLGADIIEAGFPASSRGDFLAVQEI 60
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL 120
A +++ ++ LAR V+ DID A EAL++ + RIH FIATS IH+K+KL+ E+V +
Sbjct: 61 ARTIKNCSVTGLARCVKGDIDAAWEALKDGAQPRIHVFIATSDIHLKHKLKMTREQVIER 120
Query: 121 AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY 180
AV V++A + V++SAEDA R++ FL E+ E VIDAGA+VIN+PDTVGY P E+
Sbjct: 121 AVGMVKYAKERFPIVQWSAEDACRTELPFLAEIVEKVIDAGASVINLPDTVGYLAPAEYG 180
Query: 181 ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL 240
+ +Y++ N+ NI ++S HCHDDLG+AVANSL+AI GADQ+E VNGIGERAGNAAL
Sbjct: 181 NIFKYMKENVPNIHKAKLSAHCHDDLGMAVANSLAAIENGADQIECAVNGIGERAGNAAL 240
Query: 241 EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ 300
EE+ VAL R+DFY+V+T + L EI RTS LVS LTG+ VP NKA+VG NAFAHESGIHQ
Sbjct: 241 EEIAVALHTRKDFYQVETGITLNEIKRTSDLVSKLTGMAVPRNKAVVGDNAFAHESGIHQ 300
Query: 301 DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFK 360
DG LKE++TYEII P+ +G + +VLGKHSGRHAF+ +L LG+ E + + F FK
Sbjct: 301 DGFLKEKSTYEIISPELVGVTADALVLGKHSGRHAFKDRLTALGFQFDSEEINKFFTMFK 360
Query: 361 DIADRKRWITDTDIEAIIQEELTKSNGKLKVEI----IHVTSGKVSTATVRI-SMNGEER 415
++ ++K+ ITD D+ ++I EE ++ K+ E +H + +V TAT+ + + +
Sbjct: 361 ELTEKKKEITDEDLVSLILEEKV-TDRKIGYEFLSLQVHYGTSQVPTATLSLKNQENAQL 419
Query: 416 IEVAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGR 475
I+ A G ++A+++ + + + ++ +L +YR+ S G+D+ +V VRV VNG+ GR
Sbjct: 420 IQEAATGAGSVEAIYNTLERCIDKDVELLDYRIQSNRKGEDAFAQVYVRVLVNGKESAGR 479
Query: 476 GLSTDIIEASA 486
G++ D++EASA
Sbjct: 480 GIAQDVLEASA 490
>sp|P48576|LEU1_SYNY3 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE) >gi|7447052|pir||S76101
hypothetical protein - Synechocystis sp. (strain PCC
6803) >gi|1001454|dbj|BAA10079.1| (D63999)
2-isopropylmalate synthase [Synechocystis sp.]
Length = 533
Score = 488 bits (1242), Expect = e-137
Identities = 260/501 (51%), Positives = 350/501 (68%), Gaps = 18/501 (3%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
++ IFDTTLRDGEQ+PG +LTVEEK+ IA+ LA+L VD+IEAGFP AS G+F+AV+KIA
Sbjct: 7 RILIFDTTLRDGEQSPGATLTVEEKLSIARALARLGVDIIEAGFPFASPGDFEAVQKIAQ 66
Query: 63 EV---EDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKK 119
V P I LARA +KDI A EAL+ A K+RIHTF+ATS IH+++KL+K EV
Sbjct: 67 TVGTENGPVICGLARATQKDIKAAAEALKPAAKHRIHTFLATSDIHLEHKLKKTRAEVLA 126
Query: 120 LAVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEF 179
+ + V +A D+EFS EDA RSD +FL +V EA I AGAT IN+PDTVGY TP E+
Sbjct: 127 IVPEMVAYAKSLVNDIEFSPEDAGRSDPEFLYQVLEAAISAGATTINIPDTVGYTTPAEY 186
Query: 180 YELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAA 239
L++ + N+ NI ISVH H+DLGLAVAN L A++ GA Q+E T+NGIGERAGNAA
Sbjct: 187 GALIKGIADNVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGERAGNAA 246
Query: 240 LEEVIVALDVRRDFYK-----------VKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVG 288
LEE+++AL VRR ++ T+++ K I TS+LVS LTG+ V PNKAIVG
Sbjct: 247 LEELVMALHVRRSYFNPFLGRPADSTAPLTNIDTKHIYATSRLVSELTGMMVQPNKAIVG 306
Query: 289 GNAFAHESGIHQDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLT 348
NAFAHESGIHQDGVLK + TYEI+D + +G + ++IVLGK SGR+AF +L+ELG+ L+
Sbjct: 307 ANAFAHESGIHQDGVLKNKLTYEIMDAESIGLTNNQIVLGKLSGRNAFGTRLKELGFDLS 366
Query: 349 EEHLERAFKKFKDIADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVTSGKVSTATVRI 408
+ L AF +FK++AD+++ ITD D+EAI+ +E+ + ++E + V+ G+ S T +
Sbjct: 367 DTELNNAFIRFKEVADKRKEITDWDLEAIVNDEIRQPPELFRLERVQVSCGEPSVPTATL 426
Query: 409 SMN---GEERIEVAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRV 465
++ G E VA GP+DA++ AIN+ + +L EY V+SVT G D+LG+V VR+
Sbjct: 427 TIRTPAGPEETAVA-IGTGPVDAVYKAINQIVQLPNELLEYSVTSVTEGIDALGKVSVRL 485
Query: 466 EVNGEIFVGRGLSTDIIEASA 486
NG I+ G +TDII ASA
Sbjct: 486 RHNGVIYTGYAANTDIIVASA 506
>pir||G81125 2-isopropylmalate synthase NMB1070 [imported] - Neisseria
meningitidis (group B strain MD58)
>gi|7226309|gb|AAF41465.1| (AE002457) 2-isopropylmalate
synthase [Neisseria meningitidis MC58]
Length = 517
Score = 478 bits (1217), Expect = e-134
Identities = 250/488 (51%), Positives = 343/488 (70%), Gaps = 5/488 (1%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
+V IFDTTLRDGEQ+PG ++T EEK+ +A+QL KL VD+IEAGF AS G+F+AV IA
Sbjct: 6 RVIIFDTTLRDGEQSPGAAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAK 65
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
+ T+ +L+RA+E+DI +AGEA+ A K RIHTFIATSPIHM+YKL+ +P++V + AV
Sbjct: 66 TITKSTVCSLSRAIERDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAV 125
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
KAV+ A +YT+DVEFS EDA RS+ DFL E+ AVI+AGAT IN+PDTVGY+ P + E
Sbjct: 126 KAVKIAREYTDDVEFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEF 185
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
R L N V S HCH+DLGLAVANSL+A++ GA QVE TVNG+GERAGNA++EE
Sbjct: 186 FRELIAKTPNGGKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEE 245
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
+++AL VR D + ++T ++ +I +SKLVS +TG V PNKAIVG NAF+HESGIHQDG
Sbjct: 246 IVMALKVRHDLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDG 305
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSL-TEEHLERAFKKFKD 361
VLK R TYEI+ + +G++ +++ LGK SGR+AF+ KL +LG L +EE L AF +FK+
Sbjct: 306 VLKHRETYEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKE 365
Query: 362 IADRKRWITDTDIEAIIQEELTKSNG---KLKVEIIHVTSGKVSTATVRISMNGEERIEV 418
+AD+KR I D D+ A++ +E+ N K + I +G+ A + S+ GEE+
Sbjct: 366 LADKKREIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEK-RA 424
Query: 419 AWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLS 478
+ +GP+DA+F AI L+ Y V++VT G +S GE VR+ + G+G
Sbjct: 425 SATGSGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGAD 484
Query: 479 TDIIEASA 486
TD++ A+A
Sbjct: 485 TDVLVATA 492
>pir||C81895 probable 2-isopropylmalate synthase (EC 4.1.3.12) NMA1270
[imported] - Neisseria meningitidis (group A strain
Z2491) >gi|7379949|emb|CAB84524.1| (AL162755) putative
2-isopropylmalate synthase [Neisseria meningitidis
Z2491]
Length = 517
Score = 477 bits (1215), Expect = e-133
Identities = 250/488 (51%), Positives = 343/488 (70%), Gaps = 5/488 (1%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
+V IFDTTLRDGEQ+PG ++T EEK+ +A+QL KL VD+IEAGF AS G+F+AV IA
Sbjct: 6 RVIIFDTTLRDGEQSPGAAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAK 65
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
+ T+ +L+RA+E+DI +AGEA+ A K RIHTFIATSPIHM+YKL+ +P++V + AV
Sbjct: 66 TITKSTVCSLSRAIERDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAV 125
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
KAV+ A +YT+DVEFS EDA RS+ DFL E+ AVI+AGAT IN+PDTVGY+ P + E
Sbjct: 126 KAVKIAREYTDDVEFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEF 185
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
R L N V S HCH+DLGLAVANSL+A++ GA QVE TVNG+GERAGNA++EE
Sbjct: 186 FRELIVKTPNGGKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEE 245
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
+++AL VR D + ++T ++ +I +SKLVS +TG V PNKAIVG NAF+HESGIHQDG
Sbjct: 246 IVMALKVRHDLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDG 305
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSL-TEEHLERAFKKFKD 361
VLK R TYEI+ + +G++ +++ LGK SGR+AF+ KL +LG L +EE L AF +FK+
Sbjct: 306 VLKHRETYEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKE 365
Query: 362 IADRKRWITDTDIEAIIQEELTKSNG---KLKVEIIHVTSGKVSTATVRISMNGEERIEV 418
+AD+KR I D D+ A++ +E+ N K + I +G+ A + S+ GEE+
Sbjct: 366 LADKKREIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEK-RA 424
Query: 419 AWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLS 478
+ +GP+DA+F AI L+ Y V++VT G +S GE VR+ + G+G
Sbjct: 425 SATGSGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGAD 484
Query: 479 TDIIEASA 486
TD++ A+A
Sbjct: 485 TDVLVATA 492
>pir||F75391 2-isopropylmalate synthase - Deinococcus radiodurans (strain R1)
>gi|6459240|gb|AAF11047.1|AE001992_7 (AE001992)
2-isopropylmalate synthase [Deinococcus radiodurans]
Length = 522
Score = 476 bits (1213), Expect = e-133
Identities = 252/486 (51%), Positives = 336/486 (68%), Gaps = 7/486 (1%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE 63
+ IFDTTLRDGEQ+PGV+L +K+EIA LA++ VDVIEAGFPIAS G+ + V +IA E
Sbjct: 16 IRIFDTTLRDGEQSPGVALNHAQKLEIAHTLARMGVDVIEAGFPIASPGDLEGVSRIARE 75
Query: 64 VEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVK 123
V P I ALARA DI+ A + + AEK+RIHTFIATSPIHM+ KL EP+ V + AV+
Sbjct: 76 VRGPVITALARANRADIEAAAKGVEAAEKSRIHTFIATSPIHMQKKLNLEPDAVIERAVE 135
Query: 124 AVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELV 183
AV A +Y +DVEFSAEDATRS+WDFL +++A ++AGAT INVPDTVGY TPEE +L
Sbjct: 136 AVRLARQYVDDVEFSAEDATRSEWDFLSRIFKAAVEAGATTINVPDTVGYTTPEEMRKLF 195
Query: 184 RYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEV 243
YL+ + V +S HCHDDLG+AVANS++A GA Q+E T+NGIGERAGNA+LEE+
Sbjct: 196 AYLKAKLP--AHVILSSHCHDDLGMAVANSIAAAEGGARQIECTINGIGERAGNASLEEI 253
Query: 244 IVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGV 303
++A RRD Y +T + +E+ R S+LVS L+G+ V PNKAIVG NAFAHESGIHQDGV
Sbjct: 254 VMAFHTRRDVYGYETGIRTRELYRASRLVSRLSGMPVQPNKAIVGDNAFAHESGIHQDGV 313
Query: 304 LKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGY-SLTEEHLERAFKKFKDI 362
+K R TYEI++ + +G + +V+GKHSGR AFRK L +LGY + ++ ++ F +FKD+
Sbjct: 314 IKARETYEIMNAELVGREAAVLVMGKHSGRAAFRKALTDLGYGDIPDDKVQHLFGRFKDL 373
Query: 363 ADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVTSG--KVSTATVRISMNGEERIEVAW 420
ADRK I D+ A++ + + +E +TSG + A VR+ + ++
Sbjct: 374 ADRKGQIYAEDLRALV-DARSDVPQTFSLEGFQITSGMNMMPVAFVRLH-TPDGPVDATA 431
Query: 421 FKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTD 480
+GP++A F AI+K G L YR+ SVT G D+LGEV + V G G++TD
Sbjct: 432 HGDGPVEAAFQAISKITGISPTLESYRIQSVTRGDDALGEVSIHVRHGETQLNGTGVATD 491
Query: 481 IIEASA 486
I+EASA
Sbjct: 492 IVEASA 497
>sp|Q02141|LEU1_LACLA 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE) >gi|486724|pir||S35132
2-isopropylmalate synthase (EC 4.1.3.12) - Lactococcus
lactis subsp. lactis
Length = 513
Score = 476 bits (1211), Expect = e-133
Identities = 254/489 (51%), Positives = 335/489 (67%), Gaps = 7/489 (1%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
MRK+ FDT+LRDGEQTPGVS ++ EKV IAKQL K + VIEAGF AS F+AVK+I
Sbjct: 1 MRKIEFFDTSLRDGEQTPGVSFSISEKVTIAKQLEKWRISVIEAGFSAASPDSFEAVKQI 60
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL 120
A + D + ALAR V DID+A EA++ A+ +IH FIATSPIHMKYKL+ PEEV K
Sbjct: 61 ADSLNDTAVTALARCVISDIDKAVEAVKGAKYPQIHVFIATSPIHMKYKLKISPEEVLKN 120
Query: 121 AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY 180
K V +A + E VEFS EDATR++ +FL+E + +DAGAT IN+PDTVGY TPEE+
Sbjct: 121 IDKCVRYARERVEVVEFSPEDATRTELNFLLEAVQTAVDAGATYINIPDTVGYTTPEEYG 180
Query: 181 ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL 240
++ ++L N + + + S HCHDDLG+AVANSL+AI+AGA +VE TVNGIGERAGNAAL
Sbjct: 181 KIFKFLIDNTKSDREIIFSPHCHDDLGMAVANSLAAIKAGAGRVEGTVNGIGERAGNAAL 240
Query: 241 EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ 300
EE+ VAL +R+DFY+ ++ + L E A T++L+S +GI +P NKAIVG NAFAHESGIHQ
Sbjct: 241 EEIAVALHIRKDFYQAQSPLKLSETAATAELISQFSGIAIPKNKAIVGANAFAHESGIHQ 300
Query: 301 DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFK 360
DGVLK TYEII P+ +G + + LGK SGRHAF +KL EL + +E L F+KFK
Sbjct: 301 DGVLKNAETYEIITPELVGIKHNSLPLGKLSGRHAFSEKLTELNIAYDDESLAILFEKFK 360
Query: 361 DIADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAW 420
+AD+K+ ITD DI A+ E K+ ++ + + K A V++ N EE I V+
Sbjct: 361 KLADKKKEITDADIHALFTGETVKNLAGFILDNVQIDGHK---ALVQLK-NQEEEIYVSQ 416
Query: 421 FK-NGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVE--VNGEIFVGRGL 477
+ +G +DA+F AI+K + KL Y V +VT G D+ LV VE G IF +G+
Sbjct: 417 GEGSGSVDAIFKAIDKVFNHQLKLISYSVDAVTDGIDAQATTLVSVENLSTGTIFNAKGV 476
Query: 478 STDIIEASA 486
D+++ SA
Sbjct: 477 DYDVLKGSA 485
>gb|AAB81913.1| (U92974) LeuA [Lactococcus lactis]
Length = 565
Score = 476 bits (1211), Expect = e-133
Identities = 254/489 (51%), Positives = 335/489 (67%), Gaps = 7/489 (1%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
MRK+ FDT+LRDGEQTPGVS ++ EKV IAKQL K + VIEAGF AS F+AVK+I
Sbjct: 53 MRKIEFFDTSLRDGEQTPGVSFSISEKVTIAKQLEKWRISVIEAGFSAASPDSFEAVKQI 112
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL 120
A + D + ALAR V DID+A EA++ A+ +IH FIATSPIHMKYKL+ PEEV K
Sbjct: 113 ADSLNDTAVTALARCVISDIDKAVEAVKGAKYPQIHVFIATSPIHMKYKLKISPEEVLKN 172
Query: 121 AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY 180
K V +A + E VEFS EDATR++ +FL+E + +DAGAT IN+PDTVGY TPEE+
Sbjct: 173 IDKCVRYARERVEVVEFSPEDATRTELNFLLEAVQTAVDAGATYINIPDTVGYTTPEEYG 232
Query: 181 ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL 240
++ ++L N + + + S HCHDDLG+AVANSL+AI+AGA +VE TVNGIGERAGNAAL
Sbjct: 233 KIFKFLIDNTKSDREIIFSPHCHDDLGMAVANSLAAIKAGAGRVEGTVNGIGERAGNAAL 292
Query: 241 EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ 300
EE+ VAL +R+DFY+ ++ + L E A T++L+S +GI +P NKAIVG NAFAHESGIHQ
Sbjct: 293 EEIAVALHIRKDFYQAQSPLKLSETAATAELISQFSGIAIPKNKAIVGANAFAHESGIHQ 352
Query: 301 DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFK 360
DGVLK TYEII P+ +G + + LGK SGRHAF +KL EL + +E L F+KFK
Sbjct: 353 DGVLKNAETYEIITPELVGIKHNSLPLGKLSGRHAFSEKLTELNIAYDDESLAILFEKFK 412
Query: 361 DIADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAW 420
+AD+K+ ITD DI A+ E K+ ++ + + K A V++ N EE I V+
Sbjct: 413 KLADKKKEITDADIHALFTGETVKNLAGFILDNVQIDGHK---ALVQLK-NQEEEIYVSQ 468
Query: 421 FK-NGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVE--VNGEIFVGRGL 477
+ +G +DA+F AI+K + KL Y V +VT G D+ LV VE G IF +G+
Sbjct: 469 GEGSGSVDAIFKAIDKVFNHQLKLISYSVDAVTDGIDAQATTLVSVENLSTGTIFNAKGV 528
Query: 478 STDIIEASA 486
D+++ SA
Sbjct: 529 DYDVLKGSA 537
>pir||F82070 2-isopropylmalate synthase VC2490 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9657070|gb|AAF95632.1|
(AE004318) 2-isopropylmalate synthase [Vibrio cholerae]
Length = 516
Score = 447 bits (1138), Expect = e-124
Identities = 233/485 (48%), Positives = 329/485 (67%), Gaps = 3/485 (0%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
+V IFDTTLRDGEQ SLTV+EK++IA L +L VD+IEAGFP++S G+F++V+ IA
Sbjct: 4 QVIIFDTTLRDGEQALSASLTVKEKLQIAYALERLGVDIIEAGFPVSSPGDFESVQTIAK 63
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
+++ + AL+RAV KDID A EAL+ AE RIHTFI+TS IH++ KLR+ ++V +AV
Sbjct: 64 NIKNSRVCALSRAVAKDIDAAAEALKVAEAFRIHTFISTSTIHVQDKLRRSYDDVVAMAV 123
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
KAV+HA +YT+DVEFS EDA R+ D L + EA I+AGA IN+PDTVGY P EF +
Sbjct: 124 KAVKHARQYTDDVEFSCEDAGRTPIDNLCRMVEAAINAGARTINIPDTVGYTVPSEFGGI 183
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
++ L + NI ISVHCHDDLG++VANS++AI+AGA QVE T+NGIGERAGN ALEE
Sbjct: 184 IQTLFNRVPNIDKAIISVHCHDDLGMSVANSIAAIQAGARQVEGTINGIGERAGNCALEE 243
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
+ + + R++ V T + EI+RTSKLVS L + + NKAIVG NAF+H SGIHQDG
Sbjct: 244 IAMIIKTRQELLGVTTGIKHDEISRTSKLVSQLCNMPIQSNKAIVGANAFSHSSGIHQDG 303
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDI 362
+LK + TYEI+ P+ +G + L SGR A + ++ +GY+ E +L+ ++ F +
Sbjct: 304 MLKNKNTYEIMTPESIGLKNQALNLTSRSGRAAVKSHMDSMGYNENEYNLDALYEDFLKL 363
Query: 363 ADRKRWITDTDIEAIIQ-EELTKSNGKLKVEIIHVTSGKV-STATVRISMNGEERIEVAW 420
ADRK + D D+EA++ L + + K+ + V SG V +T ++++ GEE+ E A
Sbjct: 364 ADRKGQVFDYDLEALMHFSNLREEDDFYKLNYLSVQSGSVMATTSIKLLCGGEEKCEAA- 422
Query: 421 FKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTD 480
NGP+DAL+ I + G E L ++ +++ G+D LG+ + G + G G+STD
Sbjct: 423 VGNGPVDALYQCIYRITGYEIVLDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGVSTD 482
Query: 481 IIEAS 485
I+EAS
Sbjct: 483 IVEAS 487
>sp|Q58595|YB95_METJA PUTATIVE 2-ISOPROPYLMALATE/HOMOCITRATE SYNTHASE MJ1195
>gi|2127714|pir||B64449 2-isopropylmalate synthase (EC
4.1.3.12) - Methanococcus jannaschii
>gi|1591824|gb|AAB99199.1| (U67561) 2-isopropylmalate
synthase (leuA) [Methanococcus jannaschii]
Length = 518
Score = 445 bits (1133), Expect = e-124
Identities = 244/491 (49%), Positives = 332/491 (66%), Gaps = 20/491 (4%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
+VYIFDTTLRDGEQTPGVSLT EEK++IA +L L VDVIEAGFP++S GE +A+KKI +
Sbjct: 23 RVYIFDTTLRDGEQTPGVSLTPEEKIDIAIKLDDLGVDVIEAGFPVSSLGEQEAIKKICS 82
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
D I LARAV+KDID A + +RIHTFIATSP+H KYKL+K EE+ +AV
Sbjct: 83 LNLDAEICGLARAVKKDIDVAIDC----GVDRIHTFIATSPLHRKYKLKKSKEEIIDIAV 138
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
A+E+ ++ VEFSAEDATR++ D+L+EVY+ +DAGA +INVPDTVG P Y L
Sbjct: 139 DAIEYIKEHGIRVEFSAEDATRTEIDYLIEVYKKAVDAGADIINVPDTVGVMIPRAMYYL 198
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
+ L++ I V ISVHCH+D GLAVANSL+A+ AGA+QV T+NG+GER GNAALEE
Sbjct: 199 INELKKEIK----VPISVHCHNDFGLAVANSLAAVEAGAEQVHCTINGLGERGGNAALEE 254
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
V+++L Y VKT++ +++ S+LVS T I+V PNKAIVG NAFAHESGIH G
Sbjct: 255 VVMSL---MSIYGVKTNIKTQKLYEISQLVSKYTEIKVQPNKAIVGENAFAHESGIHAHG 311
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGY----SLTEEHLERAFKK 358
VL TYE I P+ +G KI+LGKH+G HA KL+ELG + ++ + K+
Sbjct: 312 VLAHALTYEPIPPELVG-QKRKIILGKHTGTHAIEAKLKELGIEVGKDINKDQFDEIVKR 370
Query: 359 FKDIADRKRWITDTDIEAIIQE---ELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEER 415
K + D+ + +TD D+EAI+++ +L K + +++E I V +G T +++ EE
Sbjct: 371 IKALGDKGKRVTDRDVEAIVEDVVGKLAKKDRVVELEQIAVMTGNRVIPTASVALKIEEE 430
Query: 416 I-EVAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVG 474
I + + GP+DA AI KA+GE+ KL+EY ++++T G D+L EV+V +E G
Sbjct: 431 IKKSSAIGVGPVDAAVKAIQKAIGEKIKLKEYHINAITGGTDALAEVIVTLEGYGREITT 490
Query: 475 RGLSTDIIEAS 485
+ S DI+ AS
Sbjct: 491 KAASEDIVRAS 501
>pir||E64106 2-isopropylmalate synthase (EC 4.1.3.12) - Haemophilus influenzae
(strain Rd KW20) >gi|1574015|gb|AAC22647.1| (U32779)
2-isopropylmalate synthase (leuA) [Haemophilus
influenzae Rd]
Length = 531
Score = 442 bits (1124), Expect = e-123
Identities = 226/487 (46%), Positives = 338/487 (68%), Gaps = 3/487 (0%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
+V IFDTTLRDGEQ SLTV+EK++IA L +L VDV+E GFP++S+G+F++V+ IA
Sbjct: 20 RVIIFDTTLRDGEQALKASLTVKEKLQIALALERLGVDVMEVGFPVSSQGDFESVQTIAR 79
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
+++ +AAL+RAV+KDID A EAL+ AE RIHTFIA+S +H++ KL++ ++V +AV
Sbjct: 80 HIKNARVAALSRAVDKDIDAAYEALKVAEAFRIHTFIASSALHVEAKLKRSFDDVVGMAV 139
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
AV+ A YT+DVEFS EDA R+ D + + EA I+AGAT +N+PDTVG+ P E+ +
Sbjct: 140 AAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAINAGATTVNIPDTVGFCLPNEYGNI 199
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
+ +R + NI ISVHCH+DLG+A ANSL+A++ GA Q+E T+NGIGERAGN +LEE
Sbjct: 200 IAQVRNCVPNIDKAVISVHCHNDLGMATANSLTAVQNGARQIECTINGIGERAGNTSLEE 259
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
V++A+ VR+DF V T +N +EI R S++VS L + + PNKAIVG NAFAH SGIHQDG
Sbjct: 260 VVMAMKVRQDFMGVDTHINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIHQDG 319
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDI 362
+LK + TYEI+ P+ +G K+ L SGR A + + ++GY+ + L++ + +F +
Sbjct: 320 MLKNKNTYEILSPETIGLKKEKLNLTARSGRAAVKGHMADMGYNEQDYDLDKLYDEFLKL 379
Query: 363 ADRKRWITDTDIEAIIQEELTKSN-GKLKVEII--HVTSGKVSTATVRISMNGEERIEVA 419
AD+K + D D+EA+ ++ + + +L ++ + H T +TA V++ ++GE+ +
Sbjct: 380 ADKKGQVFDYDLEALAFIDMQQGDEDRLVLDKLSAHSTKEYPATAFVQLKLDGEKLSTSS 439
Query: 420 WFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLST 479
NGP+DA+++AI G E K+ Y +++ G ++LG+V + VE G F G GL+T
Sbjct: 440 IGGNGPVDAVYNAILNLTGLEIKMSHYNLTAKGEGAEALGQVDIVVEHKGRKFHGVGLAT 499
Query: 480 DIIEASA 486
DI+E+SA
Sbjct: 500 DIVESSA 506
>sp|P43861|LEU1_HAEIN 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE)
Length = 515
Score = 442 bits (1124), Expect = e-123
Identities = 226/487 (46%), Positives = 338/487 (68%), Gaps = 3/487 (0%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
+V IFDTTLRDGEQ SLTV+EK++IA L +L VDV+E GFP++S+G+F++V+ IA
Sbjct: 4 RVIIFDTTLRDGEQALKASLTVKEKLQIALALERLGVDVMEVGFPVSSQGDFESVQTIAR 63
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
+++ +AAL+RAV+KDID A EAL+ AE RIHTFIA+S +H++ KL++ ++V +AV
Sbjct: 64 HIKNARVAALSRAVDKDIDAAYEALKVAEAFRIHTFIASSALHVEAKLKRSFDDVVGMAV 123
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
AV+ A YT+DVEFS EDA R+ D + + EA I+AGAT +N+PDTVG+ P E+ +
Sbjct: 124 AAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAINAGATTVNIPDTVGFCLPNEYGNI 183
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
+ +R + NI ISVHCH+DLG+A ANSL+A++ GA Q+E T+NGIGERAGN +LEE
Sbjct: 184 IAQVRNCVPNIDKAVISVHCHNDLGMATANSLTAVQNGARQIECTINGIGERAGNTSLEE 243
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
V++A+ VR+DF V T +N +EI R S++VS L + + PNKAIVG NAFAH SGIHQDG
Sbjct: 244 VVMAMKVRQDFMGVDTHINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIHQDG 303
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDI 362
+LK + TYEI+ P+ +G K+ L SGR A + + ++GY+ + L++ + +F +
Sbjct: 304 MLKNKNTYEILSPETIGLKKEKLNLTARSGRAAVKGHMADMGYNEQDYDLDKLYDEFLKL 363
Query: 363 ADRKRWITDTDIEAIIQEELTKSN-GKLKVEII--HVTSGKVSTATVRISMNGEERIEVA 419
AD+K + D D+EA+ ++ + + +L ++ + H T +TA V++ ++GE+ +
Sbjct: 364 ADKKGQVFDYDLEALAFIDMQQGDEDRLVLDKLSAHSTKEYPATAFVQLKLDGEKLSTSS 423
Query: 420 WFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLST 479
NGP+DA+++AI G E K+ Y +++ G ++LG+V + VE G F G GL+T
Sbjct: 424 IGGNGPVDAVYNAILNLTGLEIKMSHYNLTAKGEGAEALGQVDIVVEHKGRKFHGVGLAT 483
Query: 480 DIIEASA 486
DI+E+SA
Sbjct: 484 DIVESSA 490
>pir||C81270 2-isopropylmalate synthase (EC 4.1.3.12) Cj1719c [imported] -
Campylobacter jejuni (strain NCTC 11168)
>gi|6969134|emb|CAB73705.1| (AL139079) 2-isopropylmalate
synthase [Campylobacter jejuni]
Length = 511
Score = 442 bits (1124), Expect = e-123
Identities = 235/486 (48%), Positives = 331/486 (67%), Gaps = 3/486 (0%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
K+ IFDTTLRDGEQ G SL + +K++IA L L VDVIEAGFP++S+G+FKAV+KIA+
Sbjct: 5 KIIIFDTTLRDGEQALGSSLGINQKLQIALALENLGVDVIEAGFPVSSQGDFKAVQKIAS 64
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
+V++ TI AL+RA++KDID A EAL+ A+ RIHTFIATS +HM+ KL+K+ +E+ +A
Sbjct: 65 KVKNSTICALSRALDKDIDMAYEALKVAKHFRIHTFIATSTLHMQDKLKKDFDEILSMAK 124
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
+A+ A YT+DVEFS EDA R+ D L + E I AGA IN+PDTVGY P EF +
Sbjct: 125 RAIIRARSYTDDVEFSCEDAGRTPIDNLCFMVENAIKAGAKTINIPDTVGYTLPSEFANI 184
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
++ L + NI ISVHCH+DLG+A NSLSAI GA Q+E T+NG+GERAGN ALEE
Sbjct: 185 IKILFNKVPNIDKAIISVHCHNDLGMATGNSLSAILQGARQIECTINGLGERAGNCALEE 244
Query: 243 VIVALDVRRDFYK-VKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQD 301
V++A+ R+D+ K TD+ + I +TSKLVS +T +P +KAIVG NAF+H SGIHQD
Sbjct: 245 VVMAIKTRKDYLKGFYTDIKCENIFKTSKLVSAITNESIPSHKAIVGSNAFSHSSGIHQD 304
Query: 302 GVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKD 361
GVLK R TYEII P +G +++++ SGR + LE LGY +L+ +++F
Sbjct: 305 GVLKNRQTYEIISPSAIGIHENRMLMTARSGRAMIKTCLENLGYDENTYNLDDVYERFLR 364
Query: 362 IADRKRWITDTDIEAII-QEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAW 420
+AD+K + D D+EA++ ++ + +E + V SG + TA V + + E + E A
Sbjct: 365 LADKKGQVYDYDLEALMFLSYESEEENEFVLEKLSVISGNIPTACVCMRIKEELKTE-AC 423
Query: 421 FKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTD 480
NGP++A+F+ I + + L+ Y +++ +SG D+ G+V V +E G F G+GLSTD
Sbjct: 424 TGNGPVEAVFNCIARITNLKPALKAYSINAKSSGVDAQGQVDVDLEFKGRKFHGKGLSTD 483
Query: 481 IIEASA 486
+IEASA
Sbjct: 484 VIEASA 489
>gi|10957107 2-isopropylmalate synthase [Buchnera sp. APS]
>gi|11182435|sp|Q9ZEY8|LEU1_BUCAI 2-ISOPROPYLMALATE
SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE) (ALPHA-IPM
SYNTHETASE) >gi|7707821|dbj|BAA95423.1| (AP001071)
2-isopropylmalate synthase [Buchnera sp. APS]
Length = 519
Score = 441 bits (1123), Expect = e-123
Identities = 227/487 (46%), Positives = 327/487 (66%), Gaps = 3/487 (0%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
KV IFDTTLRDGEQ SL+V+EK++IA L K +D++E GFP++S G+FK+V+ I+
Sbjct: 4 KVVIFDTTLRDGEQALQASLSVKEKLQIALSLEKCGIDILEIGFPVSSPGDFKSVQTISK 63
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
+++ I +LAR +EKDID AGEA+ +++ RIH F+ATS +HM+ KL+K E+ +AV
Sbjct: 64 NIKNSRICSLARCIEKDIDAAGEAMSSSDSFRIHIFLATSTLHMESKLKKNFNEIIDMAV 123
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
+V+ A +YT+D+EFS EDATR+ D L + E +I +G IN+PDTVGY P E +
Sbjct: 124 FSVKKALRYTDDIEFSCEDATRTTMDNLCRIVETLIKSGVKTINIPDTVGYTVPNELSCI 183
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
++ L + NI ISVHCHDDLG+AV NS+SAI+AGA Q+E T+NGIGERAGN ALEE
Sbjct: 184 IKNLFERVPNIHKSIISVHCHDDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTALEE 243
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
+I+A+ VR D V T++N KEI RTSK+VS + + +P NKAIVG NAFAH SGIHQDG
Sbjct: 244 IIMAIKVREDILSVSTNINYKEIYRTSKIVSQICNMPIPSNKAIVGSNAFAHSSGIHQDG 303
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDI 362
VLK R YEI++P +G K+ L SGR A + ++E+GY+ ++ +++ + F +
Sbjct: 304 VLKNRKNYEIMEPSSIGLKEVKLNLTSRSGRAAVKHYMDEMGYNNSDYNIDELYTAFLKL 363
Query: 363 ADRKRWITDTDIEA---IIQEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVA 419
AD+K + D D+EA I +++ LK + S +STA+V++ + E +
Sbjct: 364 ADKKGQVFDYDLEALAFINKQQDEWEYFSLKFFSVQSISNSLSTASVKLLCGKKTYTESS 423
Query: 420 WFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLST 479
NGP+DA++ A+N+ L+++++ + GKD+LG+V + VE F G GL+T
Sbjct: 424 TTSNGPVDAIYQALNRIACFPIILQKFQLVAKGKGKDALGQVDILVEHKKRKFHGVGLAT 483
Query: 480 DIIEASA 486
DIIEASA
Sbjct: 484 DIIEASA 490
>emb|CAA07305.2| (AJ006878) 2-isoproylmalate synthase [Buchnera aphidicola]
Length = 519
Score = 441 bits (1122), Expect = e-123
Identities = 227/487 (46%), Positives = 327/487 (66%), Gaps = 3/487 (0%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
KV IFDTTLRDGEQ SL+V+EK++IA L K +D++E GFP++S G+FK+V+ I+
Sbjct: 4 KVVIFDTTLRDGEQALQASLSVKEKLQIALSLEKCGIDILEIGFPVSSPGDFKSVQTISK 63
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
+++ I +LAR +EKDID AGEA+ +++ RIH F+ATS +HM+ KL+K E+ +AV
Sbjct: 64 NIKNSRICSLARCIEKDIDAAGEAMSSSDSFRIHIFLATSTLHMESKLKKNFNEIIDMAV 123
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
+V+ A +YT+D+EFS EDATR+ D L + E +I +G IN+PDTVGY P E +
Sbjct: 124 FSVKKALRYTDDIEFSCEDATRTTMDNLCRIVETLIKSGVKTINIPDTVGYTVPNELSCI 183
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
++ L + NI ISVHCHDDLG+AV NS+SAI+AGA Q+E T+NGIGERAGN ALEE
Sbjct: 184 IKNLLERVPNIHKSIISVHCHDDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTALEE 243
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
+I+A+ VR D V T++N KEI RTSK+VS + + +P NKAIVG NAFAH SGIHQDG
Sbjct: 244 IIMAIKVREDILSVSTNINYKEIYRTSKIVSQICNMPIPSNKAIVGSNAFAHSSGIHQDG 303
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDI 362
VLK R YEI++P +G K+ L SGR A + ++E+GY+ ++ +++ + F +
Sbjct: 304 VLKNRKNYEIMEPSSIGLKEVKLNLTSRSGRAAVKHYMDEMGYNNSDYNIDELYIAFLKL 363
Query: 363 ADRKRWITDTDIEA---IIQEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVA 419
AD+K + D D+EA I +++ LK + S +STA+V++ + E +
Sbjct: 364 ADKKGQVFDYDLEALAFINKQQDEWEYFSLKFFSVQSISNSLSTASVKLLCGKKTYTESS 423
Query: 420 WFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLST 479
NGP+DA++ A+N+ L+++++ + GKD+LG+V + VE F G GL+T
Sbjct: 424 TTSNGPVDAIYQALNRIACFPIILQKFQLVAKGKGKDALGQVDILVEHKKRKFHGVGLAT 483
Query: 480 DIIEASA 486
DIIEASA
Sbjct: 484 DIIEASA 490
>pir||A82634 2-isopropylmalate synthase XF1818 [imported] - Xylella fastidiosa
(strain 9a5c) >gi|9106897|gb|AAF84624.1|AE004003_3
(AE004003) 2-isopropylmalate synthase [Xylella
fastidiosa]
Length = 519
Score = 436 bits (1110), Expect = e-121
Identities = 225/484 (46%), Positives = 318/484 (65%), Gaps = 1/484 (0%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
++ IFDTTLRDGEQ+PG S+ ++K+ +A+ L L VD+IE GFP +S+ +++A+ IA
Sbjct: 11 RIRIFDTTLRDGEQSPGCSMPAQQKLVMARALDALGVDIIETGFPASSQSDYEAMTLIAR 70
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
E+ PT+A L+R ++ DI+ + AL +A K R+H F++TSP+H ++KLR E+V +
Sbjct: 71 ELRRPTLAVLSRCLQADIETSARALESATKPRLHVFLSTSPLHREHKLRMSKEQVLESVH 130
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
K V + +DVEFSAEDATR++ DFL+EV I AGAT IN+PDTVG++TPEE +
Sbjct: 131 KHVSLSRTLIDDVEFSAEDATRTEEDFLIEVTRVAIAAGATTINLPDTVGFSTPEEIRNM 190
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
+ N+ V S HCHDDLGLAVANSL+AI GA Q+E T+NGIGERAGN ALEE
Sbjct: 191 FTRIIANVEGANKVIFSAHCHDDLGLAVANSLAAIEGGARQIECTINGIGERAGNCALEE 250
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
+ + L VR FY + T ++ I TS+L+ L G+ V NKA+VG NAFAHESGIHQ G
Sbjct: 251 LTMVLKVRNAFYNIDTSIHTSRIVSTSQLLQRLVGMPVQRNKAVVGANAFAHESGIHQHG 310
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDI 362
+L+ R TYEI+ P+++G+ S +VLG+HSGR A ++L LGY L EE L+ F++FK +
Sbjct: 311 MLRHRGTYEIMRPQEVGWVCSHMVLGRHSGRAAVEQRLRALGYLLEEEDLKLVFEEFKQL 370
Query: 363 ADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAWFK 422
+++R +TD D++ ++Q + T +G + G + A V +S +R+
Sbjct: 371 CEKQRLVTDVDLQVLMQ-DTTVQHGYRLASMTISDIGNRANALVELSDPQGQRVAETAQG 429
Query: 423 NGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDII 482
NGP+DALF A+ A G + +L Y+V SV G D+ GE + V N + G G S DII
Sbjct: 430 NGPVDALFGALAAATGVKLELDSYQVHSVGIGADARGEANLSVRHNDTQYEGTGTSKDII 489
Query: 483 EASA 486
EASA
Sbjct: 490 EASA 493
>sp|P09151|LEU1_ECOLI 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE) >gi|7447053|pir||B64729
2-isopropylmalate synthase (EC 4.1.3.12) - Escherichia
coli >gi|1786261|gb|AAC73185.1| (AE000117)
2-isopropylmalate synthase [Escherichia coli K12]
Length = 523
Score = 436 bits (1109), Expect = e-121
Identities = 234/488 (47%), Positives = 328/488 (66%), Gaps = 4/488 (0%)
Query: 2 RKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIA 61
++V IFDTTLRDGEQ SL+V+EK++IA L ++ VDV+E GFP++S G+F++V+ IA
Sbjct: 3 QQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQTIA 62
Query: 62 TEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLA 121
+V++ + ALAR VEKDID A E+L+ AE RIHTFIATSP+H+ KLR +EV + A
Sbjct: 63 RQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIERA 122
Query: 122 VKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYE 181
+ V+ A YT+DVEFS EDA R+ L V EA I+AGAT IN+PDTVGY P EF
Sbjct: 123 IYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAINAGATTINIPDTVGYTMPFEFAG 182
Query: 182 LVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALE 241
++ L + NI ISVH HDDLGLAV NSL+A+ AGA QVE +NGIGERAGN +LE
Sbjct: 183 IISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAVHAGARQVEGAMNGIGERAGNCSLE 242
Query: 242 EVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQD 301
EVI+A+ VR+D V T +N +EI RTS+LVS + + +P NKAIVG AFAH SGIHQD
Sbjct: 243 EVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQICNMPIPANKAIVGSGAFAHSSGIHQD 302
Query: 302 GVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKD 361
GVLK R YEI+ P+ +G + ++ L SGR A + +++E+GY +E +L+ + F
Sbjct: 303 GVLKNRENYEIMTPESIGLNQIQLNLTSRSGRAAVKHRMDEMGYKESEYNLDNLYDAFLK 362
Query: 362 IADRKRWITDTDIEAII---QEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEV 418
+AD+K + D D+EA+ +++ + +L + S ++TA V+++ E + E
Sbjct: 363 LADKKGQVFDYDLEALAFIGKQQEEPEHFRLDYFSVQSGSNDIATAAVKLACGEEVKAEA 422
Query: 419 AWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLS 478
A NGP+DA++ AIN+ +L +Y +++ GKD+LG+V + NG F G GL+
Sbjct: 423 A-NGNGPVDAVYQAINRITEYNVELVKYSLTAKGHGKDALGQVDIVANYNGRRFHGVGLA 481
Query: 479 TDIIEASA 486
TDI+E+SA
Sbjct: 482 TDIVESSA 489
>pir||S40588 hypothetical protein - Escherichia coli >gi|216492|dbj|BAA01343.1|
(D10483) 2-Isopropylmalate synthase homolog(PIR:S08431)
[Escherichia coli]
Length = 523
Score = 434 bits (1103), Expect = e-120
Identities = 233/488 (47%), Positives = 328/488 (66%), Gaps = 4/488 (0%)
Query: 2 RKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIA 61
++V IFDTTLRDGEQ SL+V+EK++IA L ++ VDV+E GFP++S G+F++V+ IA
Sbjct: 3 QQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQTIA 62
Query: 62 TEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLA 121
+V++ + ALAR VEKDID A E+L+ AE RIHTFIATSP+H+ KLR +EV + A
Sbjct: 63 RQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIERA 122
Query: 122 VKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYE 181
+ V+ A YT+DVEFS EDA R+ L V EA I+AGAT IN+PDTVGY P EF
Sbjct: 123 IYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAINAGATTINIPDTVGYTMPFEFAG 182
Query: 182 LVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALE 241
++ L + +I ISVH HDDLGLAV NSL+A+ AGA QVE +NGIGERAGN +LE
Sbjct: 183 IISGLYERVPSIGKAIISVHTHDDLGLAVGNSLAAVHAGARQVEGAMNGIGERAGNCSLE 242
Query: 242 EVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQD 301
EVI+A+ VR+D V T +N +EI RTS+LVS + + +P NKAIVG AFAH SGIHQD
Sbjct: 243 EVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQICNMPIPANKAIVGSGAFAHSSGIHQD 302
Query: 302 GVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKD 361
GVLK R YEI+ P+ +G + ++ L SGR A + +++E+GY +E +L+ + F
Sbjct: 303 GVLKNRENYEIMTPESIGLNQIQLNLTSRSGRAAVKHRMDEMGYKESEYNLDNLYDAFLK 362
Query: 362 IADRKRWITDTDIEAII---QEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEV 418
+AD+K + D D+EA+ +++ + +L + S ++TA V+++ E + E
Sbjct: 363 LADKKGQVFDYDLEALAFIGKQQEEPEHFRLDYFSVQSGSNDIATAAVKLACGEEVKAEA 422
Query: 419 AWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLS 478
A NGP+DA++ AIN+ +L +Y +++ GKD+LG+V + NG F G GL+
Sbjct: 423 A-NGNGPVDAVYQAINRITEYNVELVKYSLTAKGHGKDALGQVDIVANYNGRRFHGVGLA 481
Query: 479 TDIIEASA 486
TDI+E+SA
Sbjct: 482 TDIVESSA 489
>gi|10954458 2-isopropylmalate synthase [Buchnera aphidicola]
>gi|3282740|gb|AAD12600.1| (AF041837) 2-isopropylmalate
synthase [Buchnera aphidicola]
Length = 516
Score = 434 bits (1103), Expect = e-120
Identities = 224/487 (45%), Positives = 325/487 (65%), Gaps = 3/487 (0%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
KV IFDTTLRDGEQ SL+V+EK++IA L K +D+IE GFPI+S G+FK+V+ I+
Sbjct: 4 KVIIFDTTLRDGEQALQASLSVKEKLQIALSLEKCGIDIIEVGFPISSPGDFKSVQTISK 63
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
++++ I +LAR VEKDI+ AG+A+ +++ RIH F+ATS +HM+ KLRK +E+ +AV
Sbjct: 64 KIKNSRICSLARCVEKDIEVAGDAMSSSDFFRIHIFLATSTLHMESKLRKNFDEIIDMAV 123
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
+V+ A +YT+DVEFS EDA+R+ D L + E +I AG IN+PDTVGY P E +
Sbjct: 124 SSVKKALRYTDDVEFSCEDASRTTMDNLCRIVEKLIKAGVKTINIPDTVGYTIPNELSNI 183
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
++ L + NI ISVHCH+DLG+AV NS+SAI+AGA Q+E T+NGIGERAGN ALEE
Sbjct: 184 IKNLFERVPNIHKSIISVHCHNDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTALEE 243
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
+I+A+ VR D V T++ KEI RTS+++S + + +P NKAIVG NAFAH SGIHQDG
Sbjct: 244 IIMAIKVREDILGVSTNIVHKEIYRTSQIISQICNMPIPANKAIVGSNAFAHSSGIHQDG 303
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDI 362
VLK R YEI++P +G K+ L SGR A + ++++GY + ++ + F +
Sbjct: 304 VLKNRKNYEIMEPNTIGVKEVKLNLTSRSGRAAVKYYMDKMGYKDDDYDIDELYSAFLKL 363
Query: 363 ADRKRWITDTDIEAIIQEELTKSNGK---LKVEIIHVTSGKVSTATVRISMNGEERIEVA 419
AD+K + D D+EA+ + N + LK + S +STA+V++ + E +
Sbjct: 364 ADKKGQVFDYDLEALAIFSKKQENAEYFYLKFFSVQSISNGLSTASVKLKCGKKVYTESS 423
Query: 420 WFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLST 479
NGP+DA + A+NK + L+++++ + GKD+LG+V + V+ F G GL+T
Sbjct: 424 TTSNGPVDATYQALNKIINFPITLQKFQLVAKGKGKDALGQVDILVKYENRQFHGIGLAT 483
Query: 480 DIIEASA 486
DIIE+SA
Sbjct: 484 DIIESSA 490
>sp|P48571|LEU1_BUCRP 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE) >gi|899388|emb|CAA50615.1|
(X71612) 2-isopropylmalate synthase [Buchnera
aphidicola]
Length = 518
Score = 433 bits (1101), Expect = e-120
Identities = 226/489 (46%), Positives = 323/489 (65%), Gaps = 7/489 (1%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
+V IFDTTLRDGEQ SL+V++K++IA L +D+IE GFPI+S G+FK+V+ I+
Sbjct: 4 QVIIFDTTLRDGEQALQASLSVKQKLQIALSLENTGIDIIEVGFPISSPGDFKSVQTISK 63
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
+++ I +LAR ++KDID A EA+ ++ RIH F+ATS +HM+ KL+K +++ +A+
Sbjct: 64 NIKNSRICSLARCLDKDIDAAAEAMHSSNAFRIHIFLATSILHMESKLKKNFDQIIDMAI 123
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
+V+ A +YT+DVEFS EDA+R+ D L + E +I+AG IN+PDTVGY P E +
Sbjct: 124 TSVKRALRYTDDVEFSCEDASRTTMDNLCRIVEKLINAGVKTINIPDTVGYTVPNELSSI 183
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
+ L + + NI ISVHCH+DLG+AV NS+SAI+AGA Q+E T+NGIGERAGN ALEE
Sbjct: 184 IHNLFQRVPNIDKSIISVHCHNDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTALEE 243
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
VI+A+ VR D V T + KEI RTS+++S + + +PPNKAIVG NAFAH SGIHQDG
Sbjct: 244 VIIAIKVREDVLGVSTKIKHKEIYRTSQIISQICNLPIPPNKAIVGSNAFAHSSGIHQDG 303
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDI 362
VLK R YEI++P +G K+ L SGR A + + E+GY + ++ + F +
Sbjct: 304 VLKNRKNYEIMEPSSIGLKEVKLNLTSRSGRAAVKHYMTEMGYRECDYKIDELYASFLKL 363
Query: 363 ADRKRWITDTDIEAII-----QEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIE 417
AD+K + D D+EA+ QEEL + L + S +STA+V++ + IE
Sbjct: 364 ADKKGQVFDYDLEALAFINQQQEEL--EHFSLSFFSVQSISNGLSTASVKLLCGKKTFIE 421
Query: 418 VAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGL 477
A NGPIDA++ A+N+ L++Y++ + GKD+LG+V + VE F G GL
Sbjct: 422 SATTSNGPIDAIYQALNRITHFPIILQKYQLIAKGKGKDALGQVDILVEHKKRKFHGMGL 481
Query: 478 STDIIEASA 486
+TDIIE+SA
Sbjct: 482 ATDIIESSA 490
>gi|10954450 isopropylmalate synthase [Buchnera aphidicola]
>gi|3282732|gb|AAD12593.1| (AF041836) isopropylmalate
synthase [Buchnera aphidicola]
Length = 518
Score = 431 bits (1096), Expect = e-120
Identities = 220/487 (45%), Positives = 321/487 (65%), Gaps = 3/487 (0%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
+V IFDTTLRDGEQ SL+V++K++IA L +D+IE GFPI+S G+FK+V+ I+
Sbjct: 4 QVIIFDTTLRDGEQALQASLSVKQKLQIALSLENAGIDIIEVGFPISSPGDFKSVQTISK 63
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
+++ I +LAR + KDID A EA+ ++ RIH F+ATS +HM+ KL+K +++ +A+
Sbjct: 64 NIKNSRICSLARCLNKDIDTAAEAMSSSNTFRIHIFLATSTLHMESKLKKNFDQIIDMAI 123
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
+V+ A +YT+DVEFS EDA+R+ D L + E +I AG IN+PDTVGY P E +
Sbjct: 124 SSVKRALRYTDDVEFSCEDASRTTMDNLCRIVEQLIKAGVKTINIPDTVGYTVPNELSTI 183
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
+ L + + NI ISVHCH+DLG+AV NS+SAI+AGA Q+E T+NGIGERAGN ALEE
Sbjct: 184 IHNLFKRVPNIDQSIISVHCHNDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTALEE 243
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
VI+A+ VR D V T++ KEI RTS+++S + + +PPNKAIVG NAFAH SGIHQDG
Sbjct: 244 VIMAIKVREDILGVSTNIKHKEIYRTSQIISQICNLPIPPNKAIVGSNAFAHSSGIHQDG 303
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDI 362
VLK R YEI++P +G K+ L SGR A + + ++GY + +++ + F +
Sbjct: 304 VLKNRKNYEIMEPSSIGLKEVKLNLTSRSGRAAVKYYMTQMGYKECDYNIDELYTSFLKL 363
Query: 363 ADRKRWITDTDIEAIIQEELTKSNGK---LKVEIIHVTSGKVSTATVRISMNGEERIEVA 419
AD+K + D D+EA+ + + + L + S +STA+V++ + IE A
Sbjct: 364 ADKKGQVFDYDLEALAFINMQQEESEYFSLSFFSVQSISNGLSTASVKLLCGKKVSIESA 423
Query: 420 WFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLST 479
NGPIDA++ A+N+ L++Y++ + G+D+LG+V + VE F G GL+T
Sbjct: 424 TTSNGPIDAIYQALNRITNFPITLQKYQLVAKGKGRDALGQVDILVEYKKRKFHGVGLAT 483
Query: 480 DIIEASA 486
DIIE+SA
Sbjct: 484 DIIESSA 490
>gb|AAG31401.1| (AF197456) 2-isopropylmaltate synthase [Buchnera aphidicola]
Length = 502
Score = 427 bits (1087), Expect = e-118
Identities = 223/479 (46%), Positives = 317/479 (65%), Gaps = 7/479 (1%)
Query: 13 DGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVEDPTIAAL 72
DGEQ SL+V+EK++IA L K+ VD++E GFPI+S G+FK+V+ I+ +++ I +L
Sbjct: 1 DGEQALQASLSVKEKLQIALCLEKIGVDIMEIGFPISSPGDFKSVQIISKNIKNSRICSL 60
Query: 73 ARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEHATKYT 132
AR VEKDID AGEA+ + RIH F+ATS +HM+YKL+K E+ +A+ AV+ A +YT
Sbjct: 61 ARCVEKDIDAAGEAMSASNSFRIHIFLATSTLHMEYKLKKNFHEIIDMAIFAVKRALRYT 120
Query: 133 EDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRYLRRNISN 192
+D+EFS EDA+R+ D L + E +I G IN+PDTVGY P E +++ L + N
Sbjct: 121 DDIEFSCEDASRTTIDNLCRIVEKLIYHGVKTINIPDTVGYTVPNELSYIIKNLLERVPN 180
Query: 193 IKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRD 252
I ISVHCHDDLG+AV NS+SAI+AGA Q+E T+NGIGERAGN ALEEVI+A+ VR D
Sbjct: 181 IHKSIISVHCHDDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTALEEVIMAIKVRED 240
Query: 253 FYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKERTTYEI 312
V T++N KEI TSK++S + + +PPNKAIVG NAF+H SGIHQDGVLK R YEI
Sbjct: 241 ILGVSTNINYKEIYHTSKIISRICNMPIPPNKAIVGSNAFSHSSGIHQDGVLKNRKNYEI 300
Query: 313 IDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIADRKRWITDT 372
IDP +GF K+ L SGR A + + E+GY T+ +++ + F +AD+K + D
Sbjct: 301 IDPSSIGFKKLKLNLTSRSGRAAVKYYMSEMGYKDTDYNIDELYADFLKLADKKGQVFDY 360
Query: 373 DIEAII-----QEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAWFKNGPID 427
D+E++ EEL + LK + S +STA++++ + IE + NGP+D
Sbjct: 361 DLESLAFISKHPEEL--EHFCLKFFSVQSISNGLSTASIKLLCGQKLYIESSTTSNGPVD 418
Query: 428 ALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEASA 486
A++ A+NK L+++++ + GKD+LG+V + +E F G GL+TD IE+SA
Sbjct: 419 AIYQALNKITNFPIILQKFQLIAKGKGKDALGQVNILIEYKKRKFHGIGLATDFIESSA 477
>sp|O04973|LU1A_LYCPN 2-ISOPROPYLMALATE SYNTHASE A (ALPHA-ISOPROPYLMALATE SYNTHASE A)
(ALPHA-IPM SYNTHETASE A) >gi|2213882|gb|AAB61598.1|
(AF004165) 2-isopropylmalate synthase [Lycopersicon
pennellii]
Length = 589
Score = 425 bits (1080), Expect = e-118
Identities = 231/479 (48%), Positives = 324/479 (67%), Gaps = 18/479 (3%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE 63
V IFDTTLRDGEQ+PG ++T +EK+++A+QLAKL VD+IEAGFP +SE +F++VK IA E
Sbjct: 48 VRIFDTTLRDGEQSPGATMTTKEKLDVARQLAKLGVDIIEAGFPASSEADFESVKLIAEE 107
Query: 64 VED--------PTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPE 115
+ + P I L+R + DID+A EA++ A+K R+HTFIATS IHMKYKL+ E
Sbjct: 108 IGNNTDENGFVPVICGLSRCNKSDIDKAWEAVKYAKKPRVHTFIATSEIHMKYKLKMSRE 167
Query: 116 EVKKLAVKAVEHATKY-TEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYA 174
+V + A V +A EDVEFS EDA RSD +FL ++ VI AGAT +N+PDTVGY
Sbjct: 168 QVVEKARSMVAYARSLGCEDVEFSPEDAGRSDREFLYDILGEVIKAGATTLNIPDTVGYT 227
Query: 175 TPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGER 234
P EF +L+ ++ N I+ V IS HC +DLGL+ AN+L+ AGA Q+EVT+NGIGER
Sbjct: 228 VPSEFGQLITDIKANTPGIENVIISTHCQNDLGLSTANTLAGACAGARQLEVTINGIGER 287
Query: 235 AGNAALEEVIVALDVRRD--FYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAF 292
AGNA+LEEV++AL R + + T +N + I +SK+V +G++V P+KAIVG NAF
Sbjct: 288 AGNASLEEVVMALKCRGEQVLGGLYTGINTQHIVPSSKMVEEYSGLQVQPHKAIVGANAF 347
Query: 293 AHESGIHQDGVLKERTTYEIIDPKKLGFSGSK---IVLGKHSGRHAFRKKLEELGYSLTE 349
AHESGIHQDG+LK + TYEII P +G S S IVLGK SGRHA + K+ ELGY +
Sbjct: 348 AHESGIHQDGMLKHKDTYEIISPDDVGLSRSNEAGIVLGKLSGRHALKSKMLELGYDIDG 407
Query: 350 EHLERAFKKFKDIADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVTSGK--VSTATVR 407
+ LE F +FK +A++K+ ITD D+ A++ +E+ + N K+ + + G +STATV+
Sbjct: 408 KELEDLFWRFKSVAEKKKKITDDDLIALMSDEVLQPNVYWKLGDVQIMCGSLGLSTATVK 467
Query: 408 -ISMNGEERIEVAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRV 465
I+ +G+E I + GP+DA + A++ + L EY +++VT G D++ V +
Sbjct: 468 LINTDGQEHIACS-VGTGPVDAAYKAVDLIVKVPITLLEYSMNAVTEGIDAIASTRVSI 525
>gb|AAG31404.1| (AF197457) 2-isopropylmaltate synthase [Buchnera aphidicola]
Length = 506
Score = 424 bits (1078), Expect = e-117
Identities = 218/477 (45%), Positives = 318/477 (65%), Gaps = 3/477 (0%)
Query: 13 DGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVEDPTIAAL 72
DGEQ SL+V+EK++IA L K +D++E GFP++S G+FK+V+ I+ +++ I +L
Sbjct: 1 DGEQALQASLSVKEKLQIALSLEKCGIDILEIGFPVSSPGDFKSVQTISKNIKNSRICSL 60
Query: 73 ARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEHATKYT 132
AR +EKDID AGEA+ +++ RIH F+ATS +HM+ KL+K E+ +AV +V+ A +YT
Sbjct: 61 ARCIEKDIDAAGEAMSSSDSFRIHIFLATSTLHMESKLKKNFNEIIDMAVFSVKKALRYT 120
Query: 133 EDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRYLRRNISN 192
+D+EFS EDATR+ D L + E +I +G IN+PDTVGY P E +++ L + N
Sbjct: 121 DDIEFSCEDATRTTMDNLCRIVETLIKSGVKTINIPDTVGYTVPNELSCIIKNLFERVPN 180
Query: 193 IKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRD 252
I ISVHCHDDLG+AV NS+SAI+AGA Q+E T+NGIGERAGN ALEE+I+A+ VR D
Sbjct: 181 IHKSIISVHCHDDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTALEEIIMAIKVRED 240
Query: 253 FYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKERTTYEI 312
V T++N KEI RTSK+VS + + +P NKAIVG NAFAH SGIHQDGVLK R YEI
Sbjct: 241 ILSVSTNINYKEIYRTSKIVSQICNMPIPSNKAIVGSNAFAHSSGIHQDGVLKNRKNYEI 300
Query: 313 IDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIADRKRWITDT 372
++P +G K+ L SGR A + ++E+GY+ ++ +++ + F +AD+K + D
Sbjct: 301 MEPSSIGLKEVKLNLTSRSGRAAVKHYMDEMGYNNSDYNIDELYIAFLKLADKKGQVFDY 360
Query: 373 DIEA---IIQEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAWFKNGPIDAL 429
D+EA I +++ LK + S +STA+V++ + E + NGP+DA+
Sbjct: 361 DLEALAFINKQQDEWEYFSLKFFSVQSISNSLSTASVKLLCGKKTYTESSTTSNGPVDAI 420
Query: 430 FSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEASA 486
+ A+N+ L+++++ + GKD+LG+V + VE F G GL+TDIIEASA
Sbjct: 421 YQALNRIACFPIILQKFQLVAKGKGKDALGQVDILVEHKKRKFHGVGLATDIIEASA 477
>gb|AAF36732.1| (AC016662) putative 2-isopropylmalate synthase; 30920-27612
[Arabidopsis thaliana]
Length = 631
Score = 423 bits (1075), Expect = e-117
Identities = 240/513 (46%), Positives = 337/513 (64%), Gaps = 32/513 (6%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE 63
V IFDTTLRDGEQ+PG +LT +EK++IA+QLAKL VD+IEAGFP AS+ +F+AVK IA
Sbjct: 87 VRIFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTIAET 146
Query: 64 VED--------PTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPE 115
V + P I L+R +KDI+ A EA++ A++ RIHTFIATS IH+KYKL+K E
Sbjct: 147 VGNTVDENGYVPVICGLSRCNKKDIETAWEAVKYAKRPRIHTFIATSDIHLKYKLKKSKE 206
Query: 116 EVKKLAVKAVEHATKY-TEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYA 174
EV ++A V A EDVEFS EDA RS+ ++L E+ VI AGAT +N+PDTVG
Sbjct: 207 EVIEIARNMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATTLNIPDTVGIT 266
Query: 175 TPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGER 234
P EF +L+ ++ N I+ V IS HC +DLGL+ AN+LS +GA QVEVT+NGIGER
Sbjct: 267 LPSEFGQLIADIKANTPGIQNVIISTHCQNDLGLSTANTLSGAHSGARQVEVTINGIGER 326
Query: 235 AGNAALEEVIVALDVRRDFY--KVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAF 292
AGNA+LEEV++A+ R D + T ++ + I TSK+V TG++ P+KAIVG NAF
Sbjct: 327 AGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAF 386
Query: 293 AHESGIHQDGVLKERTTYEIIDPKKLGFSGSK---IVLGKHSGRHAFRKKLEELGYSLTE 349
AHESGIHQDG+LK + TYEI+ P+++G S IVLGK SGRHA + +L ELGY L +
Sbjct: 387 AHESGIHQDGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRHALKDRLNELGYVLDD 446
Query: 350 EHLERAFKKFKDIADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVTSGK--VSTATVR 407
L F +FK +A++K+ +TD D+ A++ +E+ + K+ + +T G +ST+TV+
Sbjct: 447 GQLSNLFWRFKAVAEQKKRVTDADLIALVSDEVFQPEAVWKLLDMQITCGTLGLSTSTVK 506
Query: 408 IS-MNGEERIEVAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLG--EVLVR 464
++ +G+E + + GP+DA + A++ + E L EY +++VT G D++ VL+R
Sbjct: 507 LADSDGKEHVACS-VGTGPVDAAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIR 565
Query: 465 VE--------VNGE----IFVGRGLSTDIIEAS 485
+ V GE F G G DI+ +S
Sbjct: 566 GDNNYSSTNAVTGESVERTFSGTGAGMDIVVSS 598
>gb|AAG31392.1| (AF197453) 2-isopropylmaltate synthase [Buchnera aphidicola]
Length = 502
Score = 418 bits (1063), Expect = e-116
Identities = 220/479 (45%), Positives = 318/479 (65%), Gaps = 7/479 (1%)
Query: 13 DGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVEDPTIAAL 72
DGEQ SL+V+EK++IA L K VD++E GFPI+S G+FK+V+ I+ +++ I +L
Sbjct: 1 DGEQALQASLSVKEKLQIALCLEKAKVDIMEVGFPISSPGDFKSVQTISKNIKNSRICSL 60
Query: 73 ARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEHATKYT 132
AR +EKDID AGEA+ + RIH F+ATS +HM+ KLRK E+ +AV +V+ A +YT
Sbjct: 61 ARCIEKDIDAAGEAMSVCDSFRIHLFLATSILHMESKLRKNFNEIIDMAVFSVKKALRYT 120
Query: 133 EDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRYLRRNISN 192
+D+EFS EDA+RS D L + E +I+ G IN+PDTVGYA P EF +++ L + N
Sbjct: 121 DDIEFSCEDASRSTIDNLCRIVEVLINTGVKTINIPDTVGYAVPNEFSFIIKSLFERVPN 180
Query: 193 IKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRD 252
I ISVHCHDDLG+AV NS+SAI+AGA Q+E T+NGIGERAGN ALEE+I+A+ VR +
Sbjct: 181 IHKSVISVHCHDDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTALEEIIMAIKVREN 240
Query: 253 FYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKERTTYEI 312
V T++N KEI RTS++VS + + +P NK+IVG NAF+H SGIHQDGVLK R YEI
Sbjct: 241 LLGVSTNINHKEIYRTSQIVSRICNMPIPSNKSIVGSNAFSHSSGIHQDGVLKNRENYEI 300
Query: 313 IDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIADRKRWITDT 372
+DP +G K+ L SGR A + + ++GY ++ +++ + F +AD+K + D
Sbjct: 301 MDPISIGVKKVKLNLTSRSGRAAVKYYMNQMGYKDSDYNIDELYVDFLKLADKKGQVFDY 360
Query: 373 DIEAII-----QEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAWFKNGPID 427
D+E++ Q+EL + LK + S +STA+V++ + E + NGP+D
Sbjct: 361 DLESLAFINKQQDEL--EHFYLKFFSVQSISNSLSTASVKLLCGKDLHTESSTTSNGPVD 418
Query: 428 ALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEASA 486
A++ A+NK L+++++ + GKD+LG+V + VE F G GL+TDIIE+SA
Sbjct: 419 AIYQALNKITHFPIVLQKFQLIAKGKGKDALGQVDIIVEYKKRKFHGVGLATDIIESSA 477
>gb|AAG31395.1| (AF197454) 2-isopropylmaltate synthase [Buchnera aphidicola]
Length = 503
Score = 417 bits (1060), Expect = e-115
Identities = 219/477 (45%), Positives = 314/477 (64%), Gaps = 3/477 (0%)
Query: 13 DGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVEDPTIAAL 72
DGEQ SL+V+EK++IA L K VD++E GFPI+S G+FK+V+ I+ ++++ + +L
Sbjct: 1 DGEQALQASLSVKEKLQIALCLEKSGVDIMEVGFPISSPGDFKSVQTISQKIKNSRVCSL 60
Query: 73 ARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEHATKYT 132
AR +EKDID AGEA+ + RIH F+ATS +HM+ KLRK E+ +A+ +V+ A +YT
Sbjct: 61 ARCIEKDIDVAGEAMSASNSFRIHIFLATSTLHMESKLRKNFNEIIDMAIFSVKRALRYT 120
Query: 133 EDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRYLRRNISN 192
+D+EFS EDA+R+ D L + E +I G IN+PDTVGY P E +++ L + N
Sbjct: 121 DDIEFSCEDASRTTIDNLCRIVEKLISCGVKTINIPDTVGYTIPNELSFIIKNLFEKVPN 180
Query: 193 IKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRD 252
I ISVHCHDDLG+AV NS+SAI+AGA Q+E T+NGIGERAGN ALEEVI+A+ VR D
Sbjct: 181 IHKSTISVHCHDDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTALEEVIMAIKVRED 240
Query: 253 FYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKERTTYEI 312
V T++N KEI RTS++VS + + +P NKAIVG NAFAH SGIHQDGVLK R YEI
Sbjct: 241 ILGVSTNINHKEIYRTSQIVSSICNMPIPSNKAIVGSNAFAHSSGIHQDGVLKNRENYEI 300
Query: 313 IDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIADRKRWITDT 372
IDP +G K+ L SGR A + + E+GY + +++ + F +AD+K + D
Sbjct: 301 IDPISIGLKKVKLNLTSRSGRAAVKHYMNEMGYKENDYNIDELYTDFLKLADKKGQVFDY 360
Query: 373 DIEAI--IQEELTKSN-GKLKVEIIHVTSGKVSTATVRISMNGEERIEVAWFKNGPIDAL 429
D+EA+ I ++ +S LK + S +STA+V + + E + NGP+DA+
Sbjct: 361 DLEALAFINKQQDESEYFSLKFFSVQSISNNLSTASVTLLCGKKIYTEASTTSNGPVDAI 420
Query: 430 FSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEASA 486
+ A+N+ L+++++ + GKD+LG+V + VE F G GL+TDI+E+SA
Sbjct: 421 YQALNRITHFPIVLQKFQLVAKGKGKDALGQVDILVEYKKRKFHGVGLATDIMESSA 477
>gb|AAG31398.1| (AF197455) 2-isopropylmaltate synthase [Buchnera aphidicola]
Length = 502
Score = 414 bits (1054), Expect = e-115
Identities = 215/477 (45%), Positives = 315/477 (65%), Gaps = 3/477 (0%)
Query: 13 DGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVEDPTIAAL 72
DGEQ SL+V+EK++IA L K VD++E GFPI+S G+FK+V+ I+ +++ I +L
Sbjct: 1 DGEQALQASLSVKEKLQIALCLEKAGVDIMEVGFPISSPGDFKSVQTISQNIKNSRICSL 60
Query: 73 ARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEHATKYT 132
AR +EKDID AGEA+ + RIH F+ATS +HM+ KLRK E+ +A+ AV+ A +YT
Sbjct: 61 ARCIEKDIDAAGEAMSISNSFRIHIFLATSTLHMESKLRKNFNEIIDMAIFAVKRALRYT 120
Query: 133 EDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRYLRRNISN 192
+D+EFS EDA+R+ D L + E +I +G IN+PDTVGY P E +++ L + N
Sbjct: 121 DDIEFSCEDASRTTIDNLCRIVEKLISSGVKTINIPDTVGYTIPNELSNIIKSLFERVPN 180
Query: 193 IKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRD 252
I ISVHCH+DLG+AV NS+SAI+AGA Q+E T+NGIGERAGN ALEE+I+A+ VR D
Sbjct: 181 IHKSIISVHCHNDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTALEEIIMAIKVRED 240
Query: 253 FYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKERTTYEI 312
+ T++N KEI RTS++VS + + +P NKAIVG NAF+H SGIHQDGVLK R YEI
Sbjct: 241 ILGLSTNINHKEIYRTSQIVSRICNVPIPSNKAIVGSNAFSHSSGIHQDGVLKNRENYEI 300
Query: 313 IDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIADRKRWITDT 372
+DP +G ++ L SGR A + ++E+GY + +++ + F +AD+K + D
Sbjct: 301 MDPSSIGLKKVRLNLTSRSGRAAVKYYMKEMGYKENDYNIDELYTDFLKLADKKGQVFDY 360
Query: 373 DIEAI--IQEELTKSN-GKLKVEIIHVTSGKVSTATVRISMNGEERIEVAWFKNGPIDAL 429
D+EA+ I ++ +S LK + S +STA+V + + E + NGP+DA+
Sbjct: 361 DLEALAFINKQQDESEYFSLKFFSVQSISNNLSTASVTLLCGKKIYTEASTTSNGPVDAI 420
Query: 430 FSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEASA 486
+ A+N+ L+++++ + GKD+LG+V + VE F G GL+TDI+E+SA
Sbjct: 421 YQALNRITHFPIILQKFQLIAKGKGKDALGQVDILVEYKKRKFHGVGLATDIMESSA 477
>gb|AAG31389.1| (AF197452) 2-isopropylmaltate synthase [Buchnera aphidicola]
Length = 503
Score = 413 bits (1050), Expect = e-114
Identities = 219/479 (45%), Positives = 318/479 (65%), Gaps = 7/479 (1%)
Query: 13 DGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVEDPTIAAL 72
DGEQ SL+VEEK+ IA L K VD++E GFPI+S G+FK+V+ I+ ++++ I +L
Sbjct: 1 DGEQALQASLSVEEKLXIALCLEKTGVDIMEVGFPISSPGDFKSVQTISKKIKNSQICSL 60
Query: 73 ARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEHATKYT 132
AR +EKDID AGEA+ + RIH F+ATS +HMK KLRK +E+ +A+ +V+ A +YT
Sbjct: 61 ARCIEKDIDIAGEAMSVCDFFRIHIFLATSRLHMKSKLRKNFDEIIDMAIFSVKRALRYT 120
Query: 133 EDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRYLRRNISN 192
+DV+FS EDA+R+ D L + E +I++G IN+PDTVGY+ P E +++ L + + N
Sbjct: 121 DDVQFSCEDASRTTIDNLCRIVEKLINSGVKTINIPDTVGYSIPNELSFIIKNLFQRVPN 180
Query: 193 IKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRD 252
I ISVHCHDDLG+AV NS+SAI+AGA Q+E T+NGIGERAGN ALEEVI+A+ VR
Sbjct: 181 IDKAVISVHCHDDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTALEEVIMAIKVREK 240
Query: 253 FYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKERTTYEI 312
V T++N KEI RTS+++S + + +P NKAIVG NAF+H SGIHQDGVLK R YEI
Sbjct: 241 NLGVYTNINHKEIYRTSQMISRICNMPIPSNKAIVGSNAFSHSSGIHQDGVLKNRENYEI 300
Query: 313 IDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIADRKRWITDT 372
IDP +G ++ L SGR A + + E+GY ++ +++ + F +AD+K + D
Sbjct: 301 IDPVSIGLKNVQLNLTSRSGRAAVKYYMNEMGYKDSDYNIDELYSDFLKLADKKGQVFDY 360
Query: 373 DIEAII-----QEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAWFKNGPID 427
D+E++ Q+EL LK + S +STA+V++ + E + NGP+D
Sbjct: 361 DLESLAFISKQQDEL--EYFCLKFFSVQSISNGLSTASVKLLCGKKVYTESSTTSNGPVD 418
Query: 428 ALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEASA 486
A++ A+N+ L+++++ + GKD+LG+V + VE F G GL+TDIIE+SA
Sbjct: 419 AIYQALNRITHFPITLQKFQLIAKGKGKDALGQVDILVEYKKRKFHGVGLATDIIESSA 477
>gb|AAG31377.1| (AF197448) 2-isopropylmaltate synthase [Buchnera aphidicola]
Length = 502
Score = 411 bits (1046), Expect = e-114
Identities = 216/479 (45%), Positives = 315/479 (65%), Gaps = 7/479 (1%)
Query: 13 DGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVEDPTIAAL 72
DGEQ SL+V+EK++IA L K VD++E GFPI+S G+FK+V+ I+ +++ I +L
Sbjct: 1 DGEQALQASLSVKEKLQIALCLEKAGVDIMEVGFPISSPGDFKSVQTISQNIKNSRICSL 60
Query: 73 ARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEHATKYT 132
AR +EKDID AGEA+ + + RIH F+ATS +HM+ KLRK +E+ +A+ +V+ A +YT
Sbjct: 61 ARCIEKDIDTAGEAMSHCDFFRIHVFLATSTLHMESKLRKNFDEIIDMAIFSVKRALRYT 120
Query: 133 EDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRYLRRNISN 192
+DVEFS EDA+R+ D L + E +I G IN+PDTVGY P E +++ L + N
Sbjct: 121 DDVEFSCEDASRTTMDNLCRIVEKLISCGVKTINIPDTVGYTIPNELSLIIKNLFERVPN 180
Query: 193 IKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRD 252
I ISVHCH+DLG+AV NS+SAI+AGA Q+E T+NGIGERAGN ALEEVI+A+ VR D
Sbjct: 181 IHKSTISVHCHNDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTALEEVIMAIKVRED 240
Query: 253 FYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKERTTYEI 312
+ T++N KEI RTS+++S + + +P NKAIVG NAF+H SGIHQDGVLK R YEI
Sbjct: 241 ILGLSTNINHKEIYRTSQVISRICNMPIPSNKAIVGSNAFSHSSGIHQDGVLKNRENYEI 300
Query: 313 IDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIADRKRWITDT 372
+DP +G K+ L SGR A + + E+GY ++ +++ + F +AD+K + D
Sbjct: 301 MDPSSIGLKKVKLNLTSRSGRAAVKYYMNEMGYKDSDYNIDELYVDFLKLADKKGQVFDY 360
Query: 373 DIEAII-----QEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAWFKNGPID 427
D+EA+ Q+EL + LK + S +STA+V + + E + NGP+
Sbjct: 361 DLEALAFINKKQDEL--EHFCLKFFSVQSISNNLSTASVTLLCGDKIYTESSTTSNGPVX 418
Query: 428 ALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEASA 486
A++ A+N+ L+++++ + GKD+LG+V + V+ F G GL+TDIIE+SA
Sbjct: 419 AIYQALNRITHFPIILQKFQLIAKGQGKDALGQVDILVKYKKRQFHGVGLATDIIESSA 477
>gb|AAG31927.1|AF200469_1 (AF200469) 2-isopropylmalate synthase [Buchnera aphidicola]
Length = 502
Score = 411 bits (1046), Expect = e-114
Identities = 216/477 (45%), Positives = 314/477 (65%), Gaps = 3/477 (0%)
Query: 13 DGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVEDPTIAAL 72
DGEQ SL+V+EK++IA L K VD++E GFPI+S G+FK+V+ I+ +++ + +L
Sbjct: 1 DGEQALQASLSVKEKLQIALCLEKSGVDIMEVGFPISSPGDFKSVQTISQKIKHSRVCSL 60
Query: 73 ARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEHATKYT 132
AR +EKDID AGEA+ ++ RIH F+ATS +HM+ KL+K E+ +A+ +V+ A +YT
Sbjct: 61 ARCIEKDIDVAGEAMSASDSFRIHIFLATSTLHMESKLKKNFNEIIDMAIFSVKRALRYT 120
Query: 133 EDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRYLRRNISN 192
+DVEFS EDA+R+ D L + E +I G IN+PDTVGY P E +++ L + N
Sbjct: 121 DDVEFSCEDASRTTIDNLCRIVEKLISCGVKTINIPDTVGYTIPNELSFIIKNLFERVPN 180
Query: 193 IKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRD 252
I ISVHCH+DLG+AV NS+SAI+AGA Q+E T+NGIGERAGN ALEEVI+A+ VR D
Sbjct: 181 IHKSTISVHCHNDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTALEEVIMAIKVRED 240
Query: 253 FYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKERTTYEI 312
+ T++N KEI RTS++VS + + +P NKAIVG NAF+H SGIHQDGVLK R YEI
Sbjct: 241 ILGLSTNINHKEIYRTSQIVSSICNMPIPSNKAIVGSNAFSHSSGIHQDGVLKNRENYEI 300
Query: 313 IDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIADRKRWITDT 372
IDP +G K+ L SGR A + + E+GY + +++ + F +AD+K + D
Sbjct: 301 IDPISIGLKKVKLNLTSRSGRAAVKYYMNEMGYKENDYNIDELYTDFLKLADKKGQVFDY 360
Query: 373 DIEAI--IQEELTKSN-GKLKVEIIHVTSGKVSTATVRISMNGEERIEVAWFKNGPIDAL 429
D+EA+ I ++ +S LK + S +STA+V + + E + NGP+DA+
Sbjct: 361 DLEALAFINKQQDESEYFSLKFFSVQSISNNLSTASVTLLCGKKIYTEASTTSNGPVDAI 420
Query: 430 FSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEASA 486
+ A+N+ L+++++ + GKD+LG+V + VE F G GL+TDI+E+SA
Sbjct: 421 YQALNRITHFPIVLQKFQLIAKGKGKDALGQVDILVEYKKRKFHGVGLATDIMESSA 477
>gb|AAG31386.1| (AF197451) 2-isopropylmaltate synthase [Buchnera aphidicola]
Length = 491
Score = 409 bits (1041), Expect = e-113
Identities = 215/468 (45%), Positives = 312/468 (65%), Gaps = 7/468 (1%)
Query: 24 VEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVEDPTIAALARAVEKDIDRA 83
V+EK++IA L K VD++E GFPI+S G+FK+V+ I+ +++ I +LAR +EKDID A
Sbjct: 1 VKEKLQIALSLEKAGVDIMEVGFPISSPGDFKSVQTISKNIKNSRICSLARCLEKDIDAA 60
Query: 84 GEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEHATKYTEDVEFSAEDAT 143
GEA+ + RIH F+ATS +HM+ KLRK E+ +AV +V+ A +YT+DVEFS EDA+
Sbjct: 61 GEAMSVCDSFRIHIFLATSTLHMESKLRKNFNEIVDMAVFSVKKALRYTDDVEFSCEDAS 120
Query: 144 RSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRYLRRNISNIKGVQISVHCH 203
R+ D L + E +I +G IN+PDTVGY+ P E +++ L + NI ISVHCH
Sbjct: 121 RTTIDNLCRIVEKLIKSGVKTINIPDTVGYSIPNELTCIIQNLFERVPNIHKSIISVHCH 180
Query: 204 DDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRDFYKVKTDVNLK 263
DDLG+AV NS+SAI+AGA Q+E T+NGIGERAGN ALEEVI+A+ VR+D V T++N K
Sbjct: 181 DDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTALEEVIMAIKVRKDILGVSTNINHK 240
Query: 264 EIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKERTTYEIIDPKKLGFSGS 323
EI RTS+++S + + +P NKAIVG NAF+H SGIHQDGVLK R YEI+DPK +G
Sbjct: 241 EIYRTSQIISRICNMPIPSNKAIVGSNAFSHSSGIHQDGVLKNRENYEIMDPKSIGLKKV 300
Query: 324 KIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIADRKRWITDTDIEAII----- 378
K+ L SGR A R + E+GY ++ +++ + F +AD+K + D D+E++
Sbjct: 301 KLNLTSRSGRAAVRYYMNEMGYKDSDYNIDELYADFLKLADKKGQVFDYDLESLAFINKQ 360
Query: 379 QEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAWFKNGPIDALFSAINKALG 438
Q+EL +L+ + S +STA+V++ + E + NGP+DA++ A+N+
Sbjct: 361 QDEL--EYFRLQFFSVQSISNNLSTASVKLLCGKKIYTESSTTSNGPVDAVYQALNRITH 418
Query: 439 EEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEASA 486
L+++++ + GKD+LG+V + VE F G GL+TDIIE+SA
Sbjct: 419 FPIILQKFQLMAKGKGKDALGQVDILVEYKKRKFHGVGLATDIIESSA 466
>sp|O04974|LU1B_LYCPN 2-ISOPROPYLMALATE SYNTHASE B (ALPHA-ISOPROPYLMALATE SYNTHASE B)
(ALPHA-IPM SYNTHETASE B) >gi|2213884|gb|AAB61599.1|
(AF004166) 2-isopropylmalate synthase [Lycopersicon
pennellii]
Length = 612
Score = 408 bits (1037), Expect = e-113
Identities = 235/513 (45%), Positives = 331/513 (63%), Gaps = 32/513 (6%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE 63
V IFDTTLRDGEQ+PG ++T +EK+++A+Q AKL VD+IEAGFP +SE + +AVK IA E
Sbjct: 71 VRIFDTTLRDGEQSPGATMTTKEKLDVARQSAKLGVDIIEAGFPASSEADLEAVKLIAKE 130
Query: 64 VED--------PTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPE 115
V + P I LAR +KDID+A EA++ A+K RIHTFIATS +HM YKL+ +
Sbjct: 131 VGNGVYEEEYVPVICGLARCNKKDIDKAWEAVKYAKKPRIHTFIATSEVHMNYKLKMSRD 190
Query: 116 EVKKLAVKAVEHATKY-TEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYA 174
+V + A V +A EDVEFS EDA RSD +FL + VI AGAT +N+PDTVGY
Sbjct: 191 QVVEKARSMVAYARSIGCEDVEFSPEDAGRSDPEFLYHILGEVIKAGATTLNIPDTVGYT 250
Query: 175 TPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGER 234
PEEF +L+ ++ N ++ V IS HC +DLGL+ AN+L+ AGA Q+EVT+NGIGER
Sbjct: 251 VPEEFGQLIAKIKANTPGVEDVIISTHCQNDLGLSTANTLAGACAGARQLEVTINGIGER 310
Query: 235 AGNAALEEVIVALDVRRD--FYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAF 292
AGNA+LEEV++AL R + + T +N + I +SK+V ++G+ V P+KAIVG NAF
Sbjct: 311 AGNASLEEVVMALKCRGEQVLGGLYTGINTQHILMSSKMVEGISGLHVQPHKAIVGANAF 370
Query: 293 AHESGIHQDGVLKERTTYEIIDPKKLGF---SGSKIVLGKHSGRHAFRKKLEELGYSLTE 349
HESGIHQDG+LK + TYEII P+ +G + S IV GK SG + K+ ELGY +
Sbjct: 371 VHESGIHQDGMLKHKDTYEIISPEDIGLNRANESGIVFGKLSGVMLCKPKMLELGYEIEG 430
Query: 350 EHLERAFKKFKDIADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVTSGK--VSTATVR 407
+ L+ F +FK +A++K+ ITD D+ A++ +E+ + +++ + VT G +STATV+
Sbjct: 431 KELDDLFWRFKSVAEKKKKITDDDLVALMSDEVFQPQFVWQLQNVQVTCGSLGLSTATVK 490
Query: 408 -ISMNGEERIEVAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLG--EVLVR 464
I +G E I + GP+DA + A++ + L EY +++VT G D++ VL+R
Sbjct: 491 LIDADGREHISCS-VGTGPVDAAYKAVDLIVKVPVTLLEYSMNAVTQGIDAIASTRVLIR 549
Query: 465 VE--------VNGE----IFVGRGLSTDIIEAS 485
E + GE F G G DI+ +S
Sbjct: 550 GENGHTSTHALTGETVHRTFSGTGADMDIVISS 582
>gb|AAG31383.1| (AF197450) 2-isopropylmaltate synthase [Buchnera aphidicola]
Length = 502
Score = 404 bits (1027), Expect = e-111
Identities = 211/477 (44%), Positives = 310/477 (64%), Gaps = 3/477 (0%)
Query: 13 DGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVEDPTIAAL 72
DGEQ+ SL+V+EK++IA L K VD+IE GFPI+S G+FK+V+ I+ ++++ I +L
Sbjct: 1 DGEQSLQASLSVKEKLQIALCLEKAGVDIIEVGFPISSPGDFKSVQTISQKIKNSKICSL 60
Query: 73 ARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEHATKYT 132
AR +EKDID A EA+ RIH F+ATS +H++ KLRK E+ ++A+ +V+ A +YT
Sbjct: 61 ARCIEKDIDTAAEAMSVCNSFRIHIFLATSTLHIESKLRKNFNEIIEMAIFSVKRALRYT 120
Query: 133 EDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRYLRRNISN 192
+D+EFS EDA+R+ D L + E +I G IN+PDTVGY P E +++ L + N
Sbjct: 121 DDIEFSCEDASRTTMDNLCRIVEKLIYCGVKTINIPDTVGYTVPNELSGVIKNLFERVPN 180
Query: 193 IKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRD 252
I ISVHCH+DLG+AV NS+SAI+AGA Q+E T+NGIGERAGN ALEEVI+A+ VR D
Sbjct: 181 IHKSIISVHCHNDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTALEEVIMAIKVRED 240
Query: 253 FYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKERTTYEI 312
V T++N KEI +TS+++S + I VP NKAIVG NAF+H SGIHQDGVLK R YEI
Sbjct: 241 ILGVSTNINHKEIYQTSQIISRICNIPVPSNKAIVGSNAFSHSSGIHQDGVLKNRENYEI 300
Query: 313 IDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIADRKRWITDT 372
+DP +G K+ L SGR A + + +GY ++ +++ + F +AD+K + D
Sbjct: 301 MDPNSIGLKKVKLNLTSRSGRAAVKYYMNYMGYKDSDYNIDELYFNFLKLADKKGQVFDY 360
Query: 373 DIEAII---QEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAWFKNGPIDAL 429
D+EA+ ++ L+ + S +STA+V++ + E + NGP++A+
Sbjct: 361 DLEALAFMNKKHDESEYFSLQFFSVQSISNNLSTASVKLLCGKKTYTESSTTSNGPVEAI 420
Query: 430 FSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEASA 486
+ A+NK L+ +++ + GKD+LGEV + VE F G GL+ +IIE++A
Sbjct: 421 YQALNKITQFPIVLQTFQLIAKGKGKDALGEVKILVEYKKRQFHGLGLAANIIESAA 477
>pir||D69064 isopropylmalate synthase - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2622597|gb|AAB85956.1| (AE000909)
isopropylmalate synthase [Methanobacterium
thermoautotrophicum]
Length = 505
Score = 404 bits (1026), Expect = e-111
Identities = 232/488 (47%), Positives = 316/488 (64%), Gaps = 19/488 (3%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE 63
V IFDTTLRDGEQTPGV+LTVEEK IA++L L VD IEAGFP AS GE ++++I
Sbjct: 16 VRIFDTTLRDGEQTPGVALTVEEKTGIARKLDALGVDTIEAGFPAASPGEMNSIRRIVEL 75
Query: 64 VEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVK 123
+ I LAR + +DID A + + IHTFI TSP+H +YKL+ P+E+ AV
Sbjct: 76 GLEANICGLARVLREDIDAAIDC----GVDYIHTFIGTSPLHREYKLKMSPDEIIDRAVF 131
Query: 124 AVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELV 183
VE+A ++ VEFSAEDATR+++D+LVEVY A DAGA +INVPDTVG P LV
Sbjct: 132 GVEYAAEHGLKVEFSAEDATRTEFDYLVEVYRAAEDAGAEIINVPDTVGVMIPRAMNHLV 191
Query: 184 RYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEV 243
L+ + V ISVHCH+D GLAVANSL+A+ AGA QV TVNG+GERAGNAALEEV
Sbjct: 192 SRLKEELR----VPISVHCHNDFGLAVANSLAAVEAGASQVHATVNGLGERAGNAALEEV 247
Query: 244 IVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGV 303
++AL R Y + D+ E+ S+ VS +TG+ +PPNKAIVG NAFAHE+GIH GV
Sbjct: 248 VMALITR---YDLPVDIRTVELVNISEFVSKITGVRMPPNKAIVGENAFAHEAGIHVHGV 304
Query: 304 LKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIA 363
L++ TYE I P+ +G +IVLGKH+G +A R KL+E G + EE +++ K +
Sbjct: 305 LEKAETYEPITPEMVGHK-RRIVLGKHTGANALRSKLQEYGIEMKEEQFCTLYEQVKRLG 363
Query: 364 DRKRWITDTDIEAIIQEELTKSNGKL-KVEIIHVTSGK--VSTATVRISMNGEERIEVAW 420
D+ + ITD D+ A+ L K++ ++ K+E I V +G+ + TATV++ + G+E +
Sbjct: 364 DKGKRITDADLRAMAVTILGKASREIVKLEGIAVMTGESVMPTATVKLRI-GDEVKTTSM 422
Query: 421 FKNGPIDALFSAINKALGE--EFKLREYRVSSVTSGKDSLGEV-LVRVEVNGEIFVGRGL 477
GP+DA +AI + E + +L EY + ++T G ++L EV +V + G GR
Sbjct: 423 TGVGPVDAAINAIQSLVSETADIELDEYNIEAITGGTNALAEVFVVMSDGEGNKATGRST 482
Query: 478 STDIIEAS 485
DI+ AS
Sbjct: 483 REDIVMAS 490
>gb|AAF26002.1|AC013354_21 (AC013354) F15H18.3 [Arabidopsis thaliana]
Length = 675
Score = 399 bits (1014), Expect = e-110
Identities = 239/557 (42%), Positives = 335/557 (59%), Gaps = 76/557 (13%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE 63
V +FDTTLRDGEQ+PG +LT +EK++IA+QLAKL VD+IEAGFP AS+ +F+AVK IA
Sbjct: 89 VRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTIAET 148
Query: 64 VED--------PTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPE 115
V + P I L+R +KDI+RA +A++ A++ RIHTFIATS IH++YKL+K
Sbjct: 149 VGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIATSDIHLEYKLKKTKA 208
Query: 116 EVKKLAVKAVEHATKY-TEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYA 174
EV ++A V A EDVEFS EDA RS+ ++L E+ VI AGAT +N+PDTVG
Sbjct: 209 EVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATTLNIPDTVGIT 268
Query: 175 TPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGER 234
P EF +L+ L+ N I+ V IS HC +DLGL+ AN+LS AGA Q+EVT+NGIGER
Sbjct: 269 LPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAGARQMEVTINGIGER 328
Query: 235 AGNAALEEVIVALDVRRDFY--KVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAF 292
AGNA+LEEV++A+ R D + T ++ + I TSK+V TG++ P+KAIVG NAF
Sbjct: 329 AGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAF 388
Query: 293 AHESGIHQDGVLKERTTYEIIDPKKLGFSGSK---IVLGKHSGRHAFRKKLEE------- 342
AHESGIHQDG+LK + TYEII P+++G S IVLGK SGRHA + +L E
Sbjct: 389 AHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHALKDRLTEVHSNLTH 448
Query: 343 ----------------------LGYSLTEEHLERAFKKFKDIADRKRWITDTDIEAIIQE 380
LGY L +E L F +FK +A++K+ +TD DI A++ +
Sbjct: 449 TNTCIAIFVGDTNVLLSFVHFQLGYQLDDEQLSTIFWRFKTVAEQKKRVTDADIIALVSD 508
Query: 381 ELTKSNGKLKVEIIHVTSGK--VSTATVRIS-MNGEERIEVAWFKNGPIDALFSAINKAL 437
E+ + K+ I +T G +STATV+++ +G+E + + GP+D+ + A++ +
Sbjct: 509 EVFQPEAVWKLLDIQITCGTLGLSTATVKLADADGKEHVACS-IGTGPVDSAYKAVDLIV 567
Query: 438 ---------------GEEFKLREYRVSSVTSGKDSLGEVLVRVE----------VNGE-- 470
E L EY +++VT G D++ V + + GE
Sbjct: 568 KVLFIPLRLSSTNNSPEPATLLEYSMNAVTEGIDAIATTRVLIRGSNKYSSTNAITGEEV 627
Query: 471 --IFVGRGLSTDIIEAS 485
F G G DI+ +S
Sbjct: 628 QRTFSGTGAGMDIVVSS 644
>gi|11497835 2-isopropylmalate synthase (leuA-2) [Archaeoglobus fulgidus]
>gi|7447060|pir||C69277 2-isopropylmalate synthase
(leuA-2) homolog - Archaeoglobus fulgidus
>gi|2650424|gb|AAB91014.1| (AE001091) 2-isopropylmalate
synthase (leuA-2) [Archaeoglobus fulgidus]
Length = 503
Score = 396 bits (1006), Expect = e-109
Identities = 217/493 (44%), Positives = 323/493 (65%), Gaps = 21/493 (4%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
M + +FDTTLRDGEQ PGVSL +E K+ IAKQL KL VDVIEAGFP A++GEF++VK+I
Sbjct: 5 MTTIRVFDTTLRDGEQMPGVSLPLEYKIRIAKQLDKLGVDVIEAGFPSATKGEFESVKEI 64
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL 120
++ + I LAR V++DID A NA + +H F+ TS I +++ ++ EE+ +
Sbjct: 65 SSLSLNAEICGLARIVKEDID----AAINANVDMVHIFVPTSRIQIEHTVKMSKEEIIEK 120
Query: 121 AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY 180
+V+ VE+ + FSA DATR++ ++L ++++AV +A ++NVPDTVG ATP +FY
Sbjct: 121 SVECVEYIKSHGVKCMFSAMDATRTEVEYLKQIFKAVENAKVDIVNVPDTVGVATPFKFY 180
Query: 181 ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL 240
EL++ LR ++ V I VHCH+D GLAVAN+ +A+ AGA++V+VTVNGIGERAGNA L
Sbjct: 181 ELIKQLREHLK----VPIDVHCHNDFGLAVANTYAAVMAGANEVQVTVNGIGERAGNADL 236
Query: 241 EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ 300
+V++ L +KT++ + + TSKLV LTG+++PPN IVG NAF+HESGIH
Sbjct: 237 AQVVMILHA---IEGIKTNIKTEHLFETSKLVERLTGVKMPPNHPIVGENAFSHESGIHA 293
Query: 301 DGVLKERTTYE--IIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKK 358
GVLKE +T+E ++ P+ +G +IV+GKH+GR+ +K LEE GY L +E L + F+K
Sbjct: 294 HGVLKEYSTFEPGVVTPEMVGHK-RRIVIGKHAGRYQIKKILEEAGYHLDDEKLNQIFEK 352
Query: 359 FKDIADRKRWITDTDI----EAIIQEELTKSNGKLKVEIIHVTSGKVS-TATVRISMNGE 413
K++ D+ + +TD D+ E +I E + L E+ +T K++ TA + + G
Sbjct: 353 VKEMGDKGKRVTDLDLFAIAEVVIGELKREEKAILVDEVTVLTGNKITPTAVLNAEVFGH 412
Query: 414 ERIEVAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVN-GEIF 472
++ A GP+DA A+ +GE ++ E+R+ ++T G D+L EV V VE + G F
Sbjct: 413 RKVTSA-IGVGPVDASLKAVTSLVGESIRITEFRMDAITGGSDALAEVYVTVEDDEGHSF 471
Query: 473 VGRGLSTDIIEAS 485
RG + DI+ AS
Sbjct: 472 TSRGAAQDIVMAS 484
>sp|Q39891|LEU1_SOYBN PROBABLE 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE
SYNTHASE) (ALPHA-IPM SYNTHETASE) (LATE NODULIN 56)
(N-56) >gi|7447056|pir||T08590 2-isopropylmalate
synthase homolog - soybean >gi|532290|dbj|BAA07212.1|
(D38015) soybean late nodulin [Glycine max]
Length = 565
Score = 393 bits (998), Expect = e-108
Identities = 216/468 (46%), Positives = 307/468 (65%), Gaps = 18/468 (3%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE 63
V I DTTLRDGEQ+PG ++T +EK++IA+QL KL VD+I+ GFP AS +F AVK IA E
Sbjct: 33 VRILDTTLRDGEQSPGATMTAKEKLDIARQLVKLGVDIIQPGFPSASNSDFMAVKMIAQE 92
Query: 64 VED--------PTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPE 115
V + P IA R VEKDI A EA++ A++ R+ T IATSPIHM++KLRK +
Sbjct: 93 VGNAVDDDGYVPVIAGFCRCVEKDISTAWEAVKYAKRPRLCTSIATSPIHMEHKLRKSKD 152
Query: 116 EVKKLAVKAVEHATKY-TEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYA 174
+V ++A V+ A D++F AEDATRSD +FL E+ VI+AGAT +N+ DTVG
Sbjct: 153 QVIQIARDMVKFARSLGCNDIQFGAEDATRSDREFLYEILGVVIEAGATTVNIADTVGIV 212
Query: 175 TPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGER 234
P E +L+ ++ N I V IS HCH+DLGLA AN++ R GA Q+EVT+NGIGER
Sbjct: 213 MPLELGKLIVDIKDNTPGIANVIISTHCHNDLGLATANTIEGARTGARQLEVTINGIGER 272
Query: 235 AGNAALEEVIVALDVRRD--FYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAF 292
AGNA+LEEV++AL + D + T +N + I TSK+V +G+ + P+K +VG NAF
Sbjct: 273 AGNASLEEVVMALASKGDHALNGLYTRINTRHILETSKMVEEYSGMHLQPHKPLVGANAF 332
Query: 293 AHESGIHQDGVLKERTTYEIIDPKKLGFSGSK---IVLGKHSGRHAFRKKLEELGYSLTE 349
H SGIHQDG+LK + TYE I P+++G + IVLGK SG A RK+LEELGY L E
Sbjct: 333 VHASGIHQDGMLKHKGTYETISPEEIGHKRTTRIGIVLGKLSGSQALRKRLEELGYDLKE 392
Query: 350 EHLERAFKKFKDIADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVTSGKV--STATVR 407
+ ++ F +FK +A++K+ +TD D++A++ + + K+ + VT G + STATV+
Sbjct: 393 DEVDSVFWQFKAMAEKKKVVTDVDLKALVSYKAFHAESIWKLGDLQVTCGTIGLSTATVK 452
Query: 408 -ISMNGEERIEVAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSG 454
++++G + + G +D+ + AIN + E KL +Y ++SVT G
Sbjct: 453 LVNIDGSTHVACS-IGIGAVDSTYKAINLIVKEPTKLLDYSLNSVTEG 499
>pir||F69196 2-isopropylmalate synthase - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2621811|gb|AAB85228.1| (AE000851)
2-isopropylmalate synthase [Methanobacterium
thermoautotrophicum]
Length = 496
Score = 390 bits (991), Expect = e-107
Identities = 222/488 (45%), Positives = 309/488 (62%), Gaps = 19/488 (3%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
+V + DTTLRDGEQTPGVSLT EEK+ IA ++ L D+IEAG I SEGE + ++KI +
Sbjct: 2 QVRVLDTTLRDGEQTPGVSLTPEEKLRIALKIDALGADIIEAGSAITSEGEREGIRKITS 61
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
E I + ARAV +DID A + + + +H + TS +H+++KLRK EEV + AV
Sbjct: 62 EGLRAEICSFARAVREDID----AAISCDVDSVHLVVPTSDLHLEHKLRKTREEVLEQAV 117
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
E+A + VE SAED+TRSD DFL ++ I+AGA I DTVG TPE YE
Sbjct: 118 DCTEYAVDHGILVELSAEDSTRSDMDFLRTIFREGIEAGAERICACDTVGILTPERSYEF 177
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
R L G +SVHCH+D GLAVANSL+ +RAGA +V T+NGIGERAGNAALEE
Sbjct: 178 YRGLSE-----LGAPLSVHCHNDFGLAVANSLAGLRAGASEVHATINGIGERAGNAALEE 232
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
V+VAL + Y V T +N++ + TS++V+ +TG+ + PNKAIVG NAFAHESGIH DG
Sbjct: 233 VVVAL---KSLYDVDTSINIEMLYETSRMVARMTGVYLQPNKAIVGENAFAHESGIHADG 289
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDI 362
VLK+ TYE I P+ +G G V+GKH G HA RK+L+ELG + ++ L F++ K +
Sbjct: 290 VLKKAETYEPITPEMVG-HGRGFVMGKHIGTHALRKRLDELGMKVADDKLMEIFRRVKTL 348
Query: 363 ADRKRWITDTDIEAIIQEELTKSNGK-LKVEIIHVTSGK--VSTATVRISMNGEERIEVA 419
D + +TD D++AI ++ L K + ++ + + SG TA+V++ ++ E +E A
Sbjct: 349 GDMGKCVTDVDLQAIAEDVLGVMEDKVVDLQEVTIVSGNRVTPTASVKLRVDDREVLE-A 407
Query: 420 WFKNGPIDALFSAINKALGE--EFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGL 477
GP+DA AI K+L + + L EY V ++T G D+L +V++++ I R
Sbjct: 408 GTGVGPVDAAIVAIKKSLEDFADITLEEYHVDAITGGTDALIDVVIKLRHGDRIISARST 467
Query: 478 STDIIEAS 485
DII AS
Sbjct: 468 QPDIIMAS 475
>sp|Q58787|YD92_METJA PUTATIVE 2-ISOPROPYLMALATE/HOMOCITRATE SYNTHASE MJ1392
>gi|2127716|pir||G64473 2-isopropylmalate synthase (EC
4.1.3.12) - Methanococcus jannaschii
>gi|1592039|gb|AAB99402.1| (U67579) 2-isopropylmalate
synthase (leuA) [Methanococcus jannaschii]
Length = 491
Score = 388 bits (985), Expect = e-106
Identities = 224/489 (45%), Positives = 319/489 (64%), Gaps = 20/489 (4%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE 63
V IFDTTLRDGEQTPGVSLT +K+EIAK+L +L VDVIEAG I S+GE + +K I E
Sbjct: 3 VRIFDTTLRDGEQTPGVSLTPNDKLEIAKKLDELGVDVIEAGSAITSKGEREGIKLITKE 62
Query: 64 VEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVK 123
+ I + RA+ DID A E + + +H + TSPIHMKYKLRK +EV + A+K
Sbjct: 63 GLNAEICSFVRALPVDIDAALEC----DVDSVHLVVPTSPIHMKYKLRKTEDEVLETALK 118
Query: 124 AVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELV 183
AVE+A ++ VE SAEDATRSD +FL++++ GA + V DTVG TP++ EL
Sbjct: 119 AVEYAKEHGLIVELSAEDATRSDVNFLIKLFNEGEKVGADRVCVCDTVGVLTPQKSQELF 178
Query: 184 RYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEV 243
+ + N++ + +SVHCH+D G+A AN+ SA+ GA Q VTVNGIGERAGNA+LEEV
Sbjct: 179 KKITENVN----LPVSVHCHNDFGMATANTCSAVLGGAVQCHVTVNGIGERAGNASLEEV 234
Query: 244 IVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGV 303
+ AL + Y T + ++++ S++VS L + VPPNKAIVG NAFAHE+GIH DG+
Sbjct: 235 VAALKI---LYGYDTKIKMEKLYEVSRIVSRLMKLPVPPNKAIVGDNAFAHEAGIHVDGL 291
Query: 304 LKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIA 363
+K TYE I P+ +G + +I+LGKHSGR A + KL+ +G ++++E L + +++ K+
Sbjct: 292 IKNTETYEPIKPEMVG-NRRRIILGKHSGRKALKYKLDLMGINVSDEQLNKIYERVKEFG 350
Query: 364 DRKRWITDTDIEAIIQEELTK-SNGKLKVEIIHVTSGKVST--ATVRISMNGEE--RIEV 418
D ++I+D D+ AI++E K K+K++ + V SG T A+V++ GE+ IE
Sbjct: 351 DLGKYISDADLLAIVREVTGKLVEEKIKLDELTVVSGNKITPIASVKLHYKGEDITLIET 410
Query: 419 AWFKNGPIDALFSAINKALG--EEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRG 476
A + GP+DA +A+ KA+ + KL EYRV ++ G D+L EV+V++ EI R
Sbjct: 411 A-YGVGPVDAAINAVRKAISGVADIKLVEYRVEAIGGGTDALIEVVVKLRKGTEIVEVRK 469
Query: 477 LSTDIIEAS 485
DII AS
Sbjct: 470 SDADIIRAS 478
>gb|AAG31926.1|AF200468_1 (AF200468) 2-isopropylmalate synthase [Buchnera aphidicola]
Length = 437
Score = 385 bits (979), Expect = e-106
Identities = 195/439 (44%), Positives = 296/439 (67%), Gaps = 7/439 (1%)
Query: 26 EKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVEDPTIAALARAVEKDIDRAGE 85
EK++IA L K+ VD++E GFPI+S G+FK+V+ I+ +++ I +LAR +EKDID AGE
Sbjct: 1 EKLQIALSLEKIGVDIMEVGFPISSPGDFKSVQTISKNIKNSRICSLARCLEKDIDAAGE 60
Query: 86 ALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEHATKYTEDVEFSAEDATRS 145
A+ + RIH F+ATS +H++ KLRK E+ ++A+ +V+ A +YT DVEFS EDA+R+
Sbjct: 61 AMSVCDNFRIHIFLATSTLHIESKLRKNFNEIIEMAIFSVKKALRYTNDVEFSCEDASRT 120
Query: 146 DWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRYLRRNISNIKGVQISVHCHDD 205
+ D L + E +I +G IN+PDTVGY+TP+E +++ L + NI ISVHCHDD
Sbjct: 121 NIDNLCRIVEKLIKSGVKTINIPDTVGYSTPDELTFIIKNLFERVPNIHKSIISVHCHDD 180
Query: 206 LGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRDFYKVKTDVNLKEI 265
LG+AV NS++AI+AGA Q+E T+NGIGERAGN ALEEVI+A+ VR++ V T++N KEI
Sbjct: 181 LGMAVGNSIAAIQAGARQIEGTINGIGERAGNTALEEVIMAIKVRKNILGVSTNINHKEI 240
Query: 266 ARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKERTTYEIIDPKKLGFSGSKI 325
RTS+++S + + +P NKAIVG NAF+H SGIHQDGVLK R YEI+DP+ +G K+
Sbjct: 241 YRTSQMISRICNMPIPSNKAIVGSNAFSHSSGIHQDGVLKNRENYEIMDPRSIGLKKVKL 300
Query: 326 VLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIADRKRWITDTDIEAII-----QE 380
L SGR A + + E+GY ++ +++ + F +AD+K + D D+E++ Q+
Sbjct: 301 NLTSRSGRAAVKYYMSEMGYKDSDYNIDELYTDFLKLADKKGQVFDYDLESLAFINKQQD 360
Query: 381 ELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAWFKNGPIDALFSAINKALGEE 440
EL +L+ + S +STA+V++ + E + NGP+DA++ A+N+
Sbjct: 361 EL--EYFRLQFFSVQSISNNLSTASVKLLCGNKTYTESSTTSNGPVDAIYQALNRITHFP 418
Query: 441 FKLREYRVSSVTSGKDSLG 459
L+++++ + GKD+LG
Sbjct: 419 IILQKFQLIAKGKGKDALG 437
>gb|AAG31380.1| (AF197449) 2-isopropylmaltate synthase [Buchnera aphidicola]
Length = 486
Score = 383 bits (972), Expect = e-105
Identities = 201/461 (43%), Positives = 293/461 (62%), Gaps = 3/461 (0%)
Query: 29 EIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVEDPTIAALARAVEKDIDRAGEALR 88
+IA L K VD++E GFPI+S G+FK+V+ I+ +++ I +LAR +EKDID AGEA+
Sbjct: 1 QIALCLEKAGVDIMEVGFPISSPGDFKSVQIISQHIKNSRICSLARCIEKDIDAAGEAMS 60
Query: 89 NAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEHATKYTEDVEFSAEDATRSDWD 148
++ RIH F+ATS +HM+ KLRK +E+ +A+ +V+ A +YT+D+EFS EDA+R+ D
Sbjct: 61 VSDAFRIHIFLATSTLHMESKLRKNFDEIIDMAIFSVKRALRYTDDIEFSCEDASRTTID 120
Query: 149 FLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGL 208
L + E +I G IN+PDTVGY P E HCHDDLG+
Sbjct: 121 NLCRIVEKLISFGVKTINIPDTVGYTVPNELSXXXXXXXXXXXXXXXXXXXXHCHDDLGM 180
Query: 209 AVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIART 268
AV NS+SAI+AGA Q+E T+NGIGERAGN ALEEVI+A+ VR D + T++N KEI RT
Sbjct: 181 AVGNSISAIQAGARQIEGTINGIGERAGNTALEEVIMAIKVREDILGLSTNINYKEIYRT 240
Query: 269 SKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKERTTYEIIDPKKLGFSGSKIVLG 328
S++VS + + +P NKAIVG NAF+H SGIHQDGVLK R YEI+DP +G K+ L
Sbjct: 241 SQIVSRICNMPIPSNKAIVGSNAFSHSSGIHQDGVLKNRENYEIMDPSSIGLKRVKLNLT 300
Query: 329 KHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIADRKRWITDTDIEAI--IQEELTKSN 386
SGR A + + E+GY + +++ + F +AD+K + D D+EA+ I ++ +S
Sbjct: 301 SRSGRAAVKYYMSEMGYKEDDYNIDELYTDFLKLADKKGQVFDYDLEALAFINKQQDESE 360
Query: 387 -GKLKVEIIHVTSGKVSTATVRISMNGEERIEVAWFKNGPIDALFSAINKALGEEFKLRE 445
LK + S +STA+V + + E + NGP+DA++ A+N+ L++
Sbjct: 361 YFSLKFFSVQSISNNLSTASVTLLCGKKIYTEASTTSNGPVDAIYQALNRITHFPIILQK 420
Query: 446 YRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEASA 486
+++ + G+D+LG+V + VE F G GL+TDI+E+SA
Sbjct: 421 FQLIAKGKGRDALGQVDILVEYKKRKFHGVGLATDIMESSA 461
>dbj|BAB08874.1| (AB026660) 2-isopropylmalate synthase-like; homocitrate
synthase-like [Arabidopsis thaliana]
Length = 506
Score = 372 bits (944), Expect = e-102
Identities = 201/403 (49%), Positives = 269/403 (65%), Gaps = 19/403 (4%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIA-- 61
V +FDTTLRDGEQ+PG SLT +K+EIA+QLAKL VD++E GFP +SE E + +K IA
Sbjct: 85 VRVFDTTLRDGEQSPGGSLTPPQKLEIARQLAKLRVDIMEVGFPGSSEEELETIKTIAKT 144
Query: 62 --TEVED-----PTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEP 114
EV++ P I A+AR +DI+ EAL+ A++ RI F +TS IHMKYKL+K
Sbjct: 145 VGNEVDEETGYVPVICAIARCKHRDIEATWEALKYAKRPRILVFTSTSDIHMKYKLKKTQ 204
Query: 115 EEVKKLAVKAVEHATKYT-EDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGY 173
EEV ++AV ++ A D++F ED RSD DFL ++ I AG TV+ + DTVG
Sbjct: 205 EEVIEMAVSSIRFAKSLGFNDIQFGCEDGGRSDKDFLCKILGEAIKAGVTVVTIGDTVGI 264
Query: 174 ATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGE 233
P E+ ELV YL+ N I V ++VHCH+DLGLA ANS++ IRAGA QVEVT+NGIGE
Sbjct: 265 NMPHEYGELVTYLKANTPGIDDVVVAVHCHNDLGLATANSIAGIRAGARQVEVTINGIGE 324
Query: 234 RAGNAALEEVIVALDVRRDFY--KVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNA 291
R+GNA+LEEV++AL R + V T ++ ++I TSK+V TG+ V +K IVG N
Sbjct: 325 RSGNASLEEVVMALKCRGAYVINGVYTKIDTRQIMATSKMVQEYTGLYVQAHKPIVGANC 384
Query: 292 FAHESGIHQDGVLKERTTYEIIDPKKLGF---SGSKIVLGKHSGRHAFRKKLEELGYSLT 348
F HESGIHQDG+LK R+TYEI+ P+ +G S +VLGK SGRHA + +L+ELGY L
Sbjct: 385 FVHESGIHQDGILKNRSTYEILSPEDIGIVKSQNSGLVLGKLSGRHAVKDRLKELGYELD 444
Query: 349 EEHLERAFKKFKDIADRKRWITDTDIEAII----QEELTKSNG 387
+E L F F+D+ K+ ITD D++A++ + L K NG
Sbjct: 445 DEKLNAVFSLFRDLTKNKKRITDADLKALVTSSDEISLEKLNG 487
>gi|11498562 2-isopropylmalate synthase (leuA-1) [Archaeoglobus fulgidus]
>gi|7447057|pir||E69369 2-isopropylmalate synthase
(leuA-1) homolog - Archaeoglobus fulgidus
>gi|2649641|gb|AAB90286.1| (AE001038) 2-isopropylmalate
synthase (leuA-1) [Archaeoglobus fulgidus]
Length = 489
Score = 367 bits (931), Expect = e-100
Identities = 210/489 (42%), Positives = 314/489 (63%), Gaps = 19/489 (3%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
+V I DTTLRDGEQTPGVSL+VE+KV IA+ L L VD+IEAG IASEG+F+A+K+I+
Sbjct: 2 QVKILDTTLRDGEQTPGVSLSVEQKVMIAEALDNLGVDIIEAGTAIASEGDFQAIKEISQ 61
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKL-RKEPEEVKKLA 121
+ I + AR +DID A +A AE I +S IH+ K K+ + V + +
Sbjct: 62 RGLNAEICSFARIKREDIDAAADA--GAES--IFMVAPSSDIHINAKFPGKDRDYVIEKS 117
Query: 122 VKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYE 181
V+A+E+A + VEF AEDA+R+D DF++++++ +A A I DTVG +PE+ E
Sbjct: 118 VEAIEYAKERGLIVEFGAEDASRADLDFVIQLFKRAEEAKADRITFADTVGVLSPEKMEE 177
Query: 182 LVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALE 241
+VR ++ + + +++HCHDD GLA AN++ I+AGA++ T+NG+GERAGNAA+E
Sbjct: 178 IVRKIKAKVK----LPLAIHCHDDFGLATANTIFGIKAGAEEFHGTINGLGERAGNAAIE 233
Query: 242 EVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQD 301
EV++AL+ Y +KT + + + TSKLV L+ + VPPNK IVG NAF HESGIH
Sbjct: 234 EVVIALEY---LYGIKTKIKKERLYNTSKLVEKLSRVVVPPNKPIVGDNAFTHESGIHTS 290
Query: 302 GVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKD 361
+ ++ +YE I P+ +G IVLGKH+GR + + ELGY T E ++ + K+
Sbjct: 291 ALFRDAKSYEPISPEVVG-RKRVIVLGKHAGRASVEAIMNELGYKATPEQMKEILARIKE 349
Query: 362 IADRKRWITDTDIEAIIQEEL-TKSNGKLKVEIIHVTSGK--VSTATVRISMNGEERIEV 418
I D+ + +TD D+ II+ L K K+K+E + + SGK + A+V++ ++G+ERIE
Sbjct: 350 IGDKGKRVTDADVRTIIETVLQIKREKKVKLEDLAIFSGKNVMPMASVKLKIDGQERIEA 409
Query: 419 AWFKNGPIDALFSAINKALGE--EFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRG 476
A GP+DA +AI +A+ E + KL Y V ++T G D+L +V+V+++ + +I RG
Sbjct: 410 A-VGLGPVDAAINAIRRAIKEFADIKLVSYHVDAITGGTDALVDVVVQLKKDNKIVTARG 468
Query: 477 LSTDIIEAS 485
TDII AS
Sbjct: 469 ARTDIIMAS 477
>dbj|BAB09819.1| (AB006708) 2-isopropylmalate synthase-like protein [Arabidopsis
thaliana]
Length = 503
Score = 359 bits (912), Expect = 3e-98
Identities = 189/401 (47%), Positives = 270/401 (67%), Gaps = 15/401 (3%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIA-- 61
V + DTTLRDGEQ+PG +LT +K+EIA+QLAKL VD++E GFP++SE EF+A+K IA
Sbjct: 85 VRVLDTTLRDGEQSPGAALTPPQKLEIARQLAKLRVDIMEVGFPVSSEEEFEAIKTIAKT 144
Query: 62 --TEVED-----PTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEP 114
EV++ P I +AR ++DI+ EAL+ A++ R+ F +TS IHMKYKL+K
Sbjct: 145 VGNEVDEETGYVPVICGIARCKKRDIEATWEALKYAKRPRVMLFTSTSEIHMKYKLKKTK 204
Query: 115 EEVKKLAVKAVEHATKYT-EDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGY 173
EEV ++AV +V++A +D++F ED R++ DF+ ++ I AGAT + DTVG
Sbjct: 205 EEVIEMAVNSVKYAKSLGFKDIQFGCEDGGRTEKDFICKILGESIKAGATTVGFADTVGI 264
Query: 174 ATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGE 233
P+EF ELV Y+ N + ++HCH+DLG+A AN++S I AGA QVEVT+NGIGE
Sbjct: 265 NMPQEFGELVAYVIENTPGADDIVFAIHCHNDLGVATANTISGICAGARQVEVTINGIGE 324
Query: 234 RAGNAALEEVIVALDVRRDFYK--VKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNA 291
R+GNA LEEV++AL R + V T ++ ++I TSK+V TG+ V P+K IVG N
Sbjct: 325 RSGNAPLEEVVMALKCRGESLMDGVYTKIDSRQIMATSKMVQEHTGMYVQPHKPIVGDNC 384
Query: 292 FAHESGIHQDGVLKERTTYEIIDPKKLGF---SGSKIVLGKHSGRHAFRKKLEELGYSLT 348
F HESGIHQDG+LK R+TYEI+ P+ +G S IVLGK SGRHA + +L+ELGY ++
Sbjct: 385 FVHESGIHQDGILKNRSTYEILSPEDVGIVKSENSGIVLGKLSGRHAVKDRLKELGYEIS 444
Query: 349 EEHLERAFKKFKDIADRKRWITDTDIEAIIQEELTKSNGKL 389
+E F +++++ K+ ITD D++A++ S+ KL
Sbjct: 445 DEKFNDIFSRYRELTKDKKRITDADLKALVVNGAEISSEKL 485
>sp|P15875|LEU1_SALTY 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE) >gi|79038|pir||S08431
2-isopropylmalate synthase (EC 4.1.3.12) - Salmonella
typhimurium >gi|47968|emb|CAA35931.1| (X51583)
isopropylmalate synthase (AA 1-522) [Salmonella
typhimurium]
Length = 522
Score = 347 bits (881), Expect = 1e-94
Identities = 205/490 (41%), Positives = 295/490 (59%), Gaps = 9/490 (1%)
Query: 2 RKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIA 61
++V IFDTTLRDGEQ SL+ +EK++IA L ++ VDV+E GFP++S G+F++V+ IA
Sbjct: 3 QQVIIFDTTLRDGEQALQASLSAKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQTIA 62
Query: 62 TEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLA 121
+++ + ALAR VEKDID A +AL+ A+ RIHTFIATSP+H+ KLR+ +EV + A
Sbjct: 63 RTIKNSRVCALARCVEKDIDVAAQALKVADAFRIHTFIATSPMHIATKLRRTLDEVIERA 122
Query: 122 VKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYE 181
V V+ A YT+DVEFS EDA R+ D L V EA I+AGA IN+PDTVGY P EF
Sbjct: 123 VYMVKRARNYTDDVEFSCEDAGRTPVDDLARVVEAAINAGARTINIPDTVGYTMPFEFAG 182
Query: 182 LVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALE 241
++ L + NI ISVH HDDLG+AV NSL+A+ AGA QVE +NGIGERAGN ALE
Sbjct: 183 IISGLYERVPNIDKAIISVHTHDDLGIAVGNSLAAVHAGARQVEGAMNGIGERAGNCALE 242
Query: 242 EVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQD 301
EVI+A+ VR+D V T++N E ++ + I P ++ F H SGIHQD
Sbjct: 243 EVIMAIKVRKDIMNVHTNINHHETGAPARPSVQICNIADPSQQSDCRQRRFRHSSGIHQD 302
Query: 302 GVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERA--FKKF 359
GVLK R YEI+ P+ +G R++ L L+ ++
Sbjct: 303 GVLKNRENYEIMTPESIG--SEPDTAEPDLPLWPCRRETSHGRDGLQGHRLQHGPPVRRV 360
Query: 360 KDIADRKRWITDTDIEA---IIQEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERI 416
+ D+K + D D+EA I +++ + +L + S ++TA+V+++ E +
Sbjct: 361 PEAGDKKGQVFDYDLEALAFINKQQEEPEHFRLDYFSVQSGSSDIATASVKLACGEEIKA 420
Query: 417 EVAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRG 476
E A NGP+DA++ AIN+ G + +L +Y +++ + + G R E + RG
Sbjct: 421 EAA-NGNGPVDAIYQAINRITGYDVELVKYDLNAKGRARRA-GSGRYRREPSWSPLPRRG 478
Query: 477 LSTDIIEASA 486
L+TDI+E+SA
Sbjct: 479 LATDIVESSA 488
>sp|Q57926|Y503_METJA PUTATIVE 2-ISOPROPYLMALATE/HOMOCITRATE SYNTHASE MJ0503
>gi|2127715|pir||G64362 2-isopropylmalate synthase (EC
4.1.3.12) - Methanococcus jannaschii
>gi|1591206|gb|AAB98494.1| (U67500) 2-isopropylmalate
synthase (leuA) [Methanococcus jannaschii]
Length = 406
Score = 315 bits (799), Expect = 6e-85
Identities = 180/387 (46%), Positives = 248/387 (63%), Gaps = 12/387 (3%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
++ +YI+DTTLRDGEQTPGV T E+K+EIA++L +L + IEAGFPI SE E VK I
Sbjct: 29 LKDIYIYDTTLRDGEQTPGVCFTKEQKLEIARKLDELGLKQIEAGFPIVSEREADIVKTI 88
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKL-RKEPEEVKK 119
A E + I AL RA++KDID+A E + + I TFIATSP+H+KYK K +E+ +
Sbjct: 89 ANEGLNADILALCRALKKDIDKAIE----CDVDGIITFIATSPLHLKYKFNNKSLDEILE 144
Query: 120 LAVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEF 179
+ V+AVE+A ++ V FSAEDATR+ + L++V++A +AGA +++ DT G ATP+
Sbjct: 145 MGVEAVEYAKEHGLFVAFSAEDATRTPIEDLIKVHKAAEEAGADRVHIADTTGCATPQSM 204
Query: 180 YELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAA 239
+ + L+ N+K I VHCH+D G AV NS+ + GA V TVNGIGERAGNAA
Sbjct: 205 EFICKTLK---ENLKKAHIGVHCHNDFGFAVINSIYGLIGGAKAVSTTVNGIGERAGNAA 261
Query: 240 LEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIH 299
LEE+I+AL V Y V +NL+ + ++V +GI++P NK IVG FAHESGIH
Sbjct: 262 LEELIMALTV---LYDVDLGLNLEVLPELCRMVEEYSGIKMPKNKPIVGELVFAHESGIH 318
Query: 300 QDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKF 359
D V++ TYE P+K+G I+LGKHSG A KL+ +G E L KK
Sbjct: 319 VDAVIENPLTYEPFLPEKIGLK-RNILLGKHSGCRAVAYKLKLMGIDYDREMLCEIVKKV 377
Query: 360 KDIADRKRWITDTDIEAIIQEELTKSN 386
K+I + ++ITD + I++E L K N
Sbjct: 378 KEIREEGKFITDEVFKEIVEEVLRKRN 404
>pir||H69084 2-isopropylmalate synthase - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2622757|gb|AAB86103.1| (AE000922)
2-isopropylmalate synthase [Methanobacterium
thermoautotrophicum]
Length = 391
Score = 296 bits (749), Expect = 5e-79
Identities = 166/369 (44%), Positives = 236/369 (62%), Gaps = 12/369 (3%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
++ I+DTTLRDGEQTPGV L EEK+EIA++L +L + IE+GFP+ SE E +VK IA
Sbjct: 19 RITIYDTTLRDGEQTPGVCLGTEEKLEIARKLDELGIHQIESGFPVVSEQERVSVKSIAN 78
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
E + I AL R + DID A + + + + TF+ATS +H+K+KL+ EE + +
Sbjct: 79 EGLNAEILALCRTKKDDID----AAIDCDVDGVITFMATSDLHLKHKLKLTREEALNVCM 134
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
++E+A + + FSAEDATR+D DFL ++Y + GA +++ DTVG +P+ L
Sbjct: 135 NSIEYAKDHGLFLAFSAEDATRTDLDFLKQIYRKAENYGADRVHIADTVGAISPQGMDYL 194
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
VR LRR+I V I++HCH+D G+A++NS++ + AG V TVNGIGERAGN +LEE
Sbjct: 195 VRELRRDIK----VDIALHCHNDFGMALSNSIAGLLAGGTAVSTTVNGIGERAGNTSLEE 250
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
+I+AL R Y+V N+ + S+LV T ++VP NK IVG N F HESGIH D
Sbjct: 251 LIMAL---RIIYEVDLGFNIGVLYELSRLVEKHTRMKVPENKPIVGRNVFRHESGIHVDA 307
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDI 362
V++E TYE P+ +G KIVLGKHSG A + KLEE G +T + L R ++ K
Sbjct: 308 VIEEPLTYEPFLPEMIGHQ-RKIVLGKHSGCRAVKAKLEEYGIDVTRDELCRIVEEVKKN 366
Query: 363 ADRKRWITD 371
++ ++I D
Sbjct: 367 REKGKYIND 375
>emb|CAA72697.1| (Y11966) 2-isopropylmalate synthase [Buchnera aphidicola]
Length = 375
Score = 286 bits (724), Expect = 4e-76
Identities = 147/346 (42%), Positives = 216/346 (61%), Gaps = 4/346 (1%)
Query: 144 RSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRYLRRNISNIKGVQISVHCH 203
R+ D L + E+ I AGAT IN+PDTVGY P +F +++ L + NI IS HCH
Sbjct: 2 RTSIDNLCRIVESAIKAGATTINIPDTVGYTIPSQFKTIIKNLFNKVPNINNAIISAHCH 61
Query: 204 DDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRDFYKVKTDVNLK 263
DDLGLAV N++++I AG Q+E T++GIGERAGN ALEEVI+ + ++ + V T++
Sbjct: 62 DDLGLAVGNAIASIEAGVRQIEGTISGIGERAGNTALEEVILTIKLKEELLNVFTNIKHN 121
Query: 264 EIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKERTTYEIIDPKKLGFSGS 323
E+ RTS+L+S L +P NK+I+G NAFAH SGIHQDG+LK R YEIIDPK +G
Sbjct: 122 ELYRTSQLISQLCNTPIPANKSIIGSNAFAHSSGIHQDGILKNRANYEIIDPKTIGVKAV 181
Query: 324 KIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIADRKRWITDTDIEAII----Q 379
++ L SGR A + + E+GY + L + + F +AD+K + D D+EA+ Q
Sbjct: 182 QLNLTSRSGRAAVKHHMHEMGYQDDDYDLNQLYVNFLKLADKKGQVFDYDLEALAFISNQ 241
Query: 380 EELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAWFKNGPIDALFSAINKALGE 439
E+ + + +LK IH S VSTA+V++ + E+ +NGPI++++ A+N G
Sbjct: 242 EDKDQEHFQLKYFSIHSGSTGVSTASVKLLCGNKILNEIITTRNGPINSIYQALNNIAGL 301
Query: 440 EFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEAS 485
L ++ + G+D+LG+V + V+ N F G GL+TDIIE+S
Sbjct: 302 PIVLEKFHLVGKGEGRDALGQVDIVVKYNNRKFHGIGLATDIIESS 347
>sp|Q56216|LEU1_THETH 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE) >gi|1293674|gb|AAB01162.1|
(U52907) isopropylmalate synthase [Thermus thermophilus]
Length = 331
Score = 280 bits (708), Expect = 3e-74
Identities = 146/296 (49%), Positives = 208/296 (69%), Gaps = 4/296 (1%)
Query: 194 KGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRDF 253
+ V IS H HDDLG+A AN+L+ + GA Q+E T+NGIGERAGN ALEEV++AL VRRD+
Sbjct: 8 RDVVISTHTHDDLGMATANALAGVENGAGQIECTINGIGERAGNCALEEVVMALYVRRDW 67
Query: 254 YKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKERTTYEII 313
YK T +N +EI R S+LV TG+ VPPNKAIVG NAFAHESGIHQDGV+K R TYEI+
Sbjct: 68 YKAYTRINTREIYRVSRLVERYTGMPVPPNKAIVGDNAFAHESGIHQDGVIKHRATYEIM 127
Query: 314 DPKKLGFSGSKIVLGKHSGRHAFRKKLEELGY-SLTEEHLERAFKKFKDIADRKRWITDT 372
D + +G + +VLGKHSGR AF+K LE+LGY L+EE +++ F +FK+IA++K ++
Sbjct: 128 DAELIGRRPAVLVLGKHSGRAAFKKALEDLGYKDLSEEEVKKLFARFKEIAEKKGPLSAE 187
Query: 373 DIEAIIQEELTKSNGKLKVEIIHVTSGK--VSTATVRISMNGEERIEVAWFKNGPIDALF 430
+++A+++ E ++ ++E + SG + TATV++ ER+ +GP+DA++
Sbjct: 188 ELQALVESEREPTSHFFQLEHVQFFSGSGLLPTATVKVKTPDGERL-ATHTGDGPVDAVY 246
Query: 431 SAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEASA 486
AI +A+G +L YRV ++T ++LG+V VR+ + VG G+S DIIEASA
Sbjct: 247 KAIQEAIGLRPELELYRVEAITGSTEALGQVTVRLRLGELQAVGVGVSPDIIEASA 302
>gb|AAK42554.1| 2-isopropylmalate synthase, putative (leuA-3) [Sulfolobus
solfataricus]
Length = 347
Score = 279 bits (706), Expect = 5e-74
Identities = 159/351 (45%), Positives = 223/351 (63%), Gaps = 15/351 (4%)
Query: 30 IAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVEDPT-IAALARAVEKDIDRAGEALR 88
IA++LA L VDVIEAGFP +SEGEF A +KI E+ D + L+R+ + DID+
Sbjct: 2 IARKLADLGVDVIEAGFPASSEGEFIATRKIFEEIGDQVEVIGLSRSNKNDIDKT----I 57
Query: 89 NAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEHATKYTEDVEFSAEDATRSDWD 148
+ + IH FIATS +HMKYKL+ EEV ++V++A + VEFS EDATR++ D
Sbjct: 58 STGISSIHLFIATSELHMKYKLKMTKEEVLNKIYESVKYAKDHGMIVEFSPEDATRTEED 117
Query: 149 FLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRYLRRNISNIKG--VQISVHCHDDL 206
FL I+AGA IN+PDTVG P ++Y+++ + I N G + +SVHCH+D
Sbjct: 118 FLFTAIRTAIEAGADRINIPDTVGTMHPFKYYDMI----KKIVNFVGERIIVSVHCHNDF 173
Query: 207 GLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIA 266
GLA ANSL+ + AGA Q VTVNGIGERAGNA+LEEV++ + + +T+V ++
Sbjct: 174 GLATANSLAGVYAGARQAHVTVNGIGERAGNASLEEVVMGI---KKLLNYETNVKTWKLY 230
Query: 267 RTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKERTTYEIIDPKKLGFSGSKIV 326
S+ V+ +TG+ VP KAIVG NAF HESGIH GV++ TYE I P+++G + ++
Sbjct: 231 EVSRFVAEMTGVPVPYFKAIVGDNAFGHESGIHVHGVIENPFTYEPISPEEVG-NFRRLA 289
Query: 327 LGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIADRKRWITDTDIEAI 377
LGKHSG H RK LEE G L+++ L+ + K +A+ +T D + I
Sbjct: 290 LGKHSGIHGLRKLLEEQGIYLSDDKLKIVLAEIKKLAESGNKVTVEDAKNI 340
>gb|AAK41252.1| 2-isopropylmalate synthase (leuA-2) [Sulfolobus solfataricus]
Length = 461
Score = 249 bits (629), Expect = 5e-65
Identities = 145/384 (37%), Positives = 219/384 (56%), Gaps = 17/384 (4%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
M KV I D+TLR+GEQTPGV TV+++VEIAK L+ L V +IEAG P S ++ +K+I
Sbjct: 1 MIKVGILDSTLREGEQTPGVIFTVDQRVEIAKALSDLGVSMIEAGHPAVSPDIYEGIKRI 60
Query: 61 ATEVED----PTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEE 116
++ I +RAV++DI+ A E E +RI F S IH+K K + EE
Sbjct: 61 VKLKKEGIITSEIVGHSRAVKRDIEIAAE----LEVDRIAIFYGVSDIHLKAKHKATREE 116
Query: 117 VKKLAVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATP 176
++ + + +A + V F+AED +R+D+DFLV V DAGA +++ DTVG P
Sbjct: 117 ALRVIAETISYARSHGVKVRFTAEDGSRTDFDFLVTVSRTARDAGADRVSIADTVGILYP 176
Query: 177 EEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAG 236
+ EL L R + N+ + +H H+DLGLAVAN+L+A+ GA V TVNG+GER G
Sbjct: 177 SKTKELFSALIREVPNL---EYDIHAHNDLGLAVANALAAVEGGATIVHATVNGLGERVG 233
Query: 237 NAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHES 296
L++++ A+ VK D ++ S L+ +GI +PPN I G AF H++
Sbjct: 234 IVPLQQIVAAIKYHFGIEVVKLD----KLQYVSSLIEKYSGIPMPPNYPITGDYAFLHKA 289
Query: 297 GIHQDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAF 356
G+H GVL + TYE + P+ G + + K++G+HA R K E+LG ++E +++
Sbjct: 290 GVHVAGVLSDPRTYEFMPPETFGRT-RDYTIDKYTGKHALRDKYEKLGVKISEAEMDQIL 348
Query: 357 KKFKDIADRKRWITDTDIEAIIQE 380
K K R+ D D+ + +E
Sbjct: 349 AKIKS-NTTIRFYRDVDLLELAEE 371
>gb|AAF13763.1|AF117351_20 (AF117351) homocitrate synthase [Zymomonas mobilis]
Length = 386
Score = 233 bits (589), Expect = 3e-60
Identities = 143/383 (37%), Positives = 208/383 (53%), Gaps = 13/383 (3%)
Query: 2 RKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIA 61
+ + I DTTLRDGEQ PGV+ T+ EK+ IAK L K VD IEAG P E +A+++I
Sbjct: 7 KHLIINDTTLRDGEQAPGVAFTLSEKLHIAKALDKAGVDEIEAGIPAMGASEIEAIRQIV 66
Query: 62 TEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRK-EPEEVKKL 120
+ + + R D+D+A E +H I++SP M K ++ L
Sbjct: 67 QQSQTARVIPWCRMKISDVDKALE----TGAKTVHLSISSSPFQMAAKFPDLSAADILGL 122
Query: 121 AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY 180
K + +A +V AEDA+R++ FL ++ + +AGA I DT+G P Y
Sbjct: 123 CQKVISYAKDRGLEVSVGAEDASRAEISFLTDLLGVIEEAGAFRIRFADTLGILDPFATY 182
Query: 181 ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL 240
E++ L + I + I H HDDLGLA ANSL+AI+AGA VTV G+GERAGNA+L
Sbjct: 183 EIISQLHQ----ISPLDIEFHGHDDLGLATANSLAAIKAGAKGASVTVLGLGERAGNASL 238
Query: 241 EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ 300
E+V++A +T + +++ ++ V+ G +PP+KAIVG F HESGIH
Sbjct: 239 EQVVIAAS---HIAGKETRIRPEKLYNLAQYVAQAAGRTIPPDKAIVGEAVFQHESGIHV 295
Query: 301 DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFK 360
G+L+ +YE ++P+KLG +IVLGKHSGR A R L LG E+ ++ K
Sbjct: 296 AGLLRNPASYEALNPEKLG-RQREIVLGKHSGRAAIRHALSLLGIKAQEDMIQNLLAAVK 354
Query: 361 DIADRKRWITDTDIEAIIQEELT 383
A ++ A+I + T
Sbjct: 355 MQASHQKRAIGLSEIALIHQNFT 377
>sp|O66682|LY41_AQUAE PUTATIVE LYASE AQ_356 >gi|7447064|pir||F70331 2-isopropylmalate
synthase - Aquifex aeolicus >gi|2983027|gb|AAC06637.1|
(AE000685) 2-isopropylmalate synthase [Aquifex aeolicus]
Length = 528
Score = 225 bits (568), Expect = 8e-58
Identities = 165/510 (32%), Positives = 258/510 (50%), Gaps = 33/510 (6%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGE---FKAVKK 59
KVYI+DTTLRDG Q GVS ++E+K+ IA++L + IE G+P A+ + F+ KK
Sbjct: 4 KVYIYDTTLRDGSQMEGVSFSLEDKIRIAEKLDDFGIHYIEGGWPYANPKDNLFFQKAKK 63
Query: 60 IATEVEDPTIAALARAVEKDI--DRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEV 117
+ + T R K + D E+L AE + F + +H+ L+ EE
Sbjct: 64 MNFKNAKLTAFGSTRRPNKKVSEDPQVESLIKAETPVVTIFGKSWDLHVTDALKTTLEEN 123
Query: 118 KKLAVKAVEHATKYTEDVEFSAE---DATRSDWDFLVEVYEAVIDAGATVINVPDTVGYA 174
+ + VE+ +Y ++V F AE D +S+ ++ ++V EA + GA + + DT G
Sbjct: 124 LNMIYETVEYLKRYVDEVIFDAEHFFDGYKSNPEYALQVLEAALKGGADWVVLCDTNGGT 183
Query: 175 TPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGER 234
P E YE+ + ++ K + +H H+D AVANSL A+ AGA QV T+NGIGER
Sbjct: 184 LPHEIYEITKKVKERF---KDANVGIHAHNDSETAVANSLMAVLAGARQVHGTINGIGER 240
Query: 235 AGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAH 294
GNA L +I L ++ F + + NLK++ + V+ + + +P N VG +AFAH
Sbjct: 241 TGNANLCSIIPNLQLKLGFDVIPQE-NLKKLTELANFVAEIINMPLPRNMPYVGESAFAH 299
Query: 295 ESGIHQDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEE--HL 352
+ G+H VLK TYE I+P+ +G + KI + +GR KL+E G + + L
Sbjct: 300 KGGVHASAVLKNAKTYEHINPELVG-NKRKITVSDLAGRSNLVHKLKEFGIEIDPKSPEL 358
Query: 353 ERAFKKFKDIADRKRWI--TDTDIEAIIQEELTKSNGKLKVEIIHVTSGK-------VST 403
++ K K++ + +E +I+ + V K S
Sbjct: 359 KKLIDKIKELEKEGYHFEAAEASLELLIKRHFGLVKDYFDFDAYRVLIAKRRDDSLPTSE 418
Query: 404 ATVRISMNGEERIEVAWFKNGPIDALFSAINKALGE------EFKLREYRVSSVTSGKDS 457
ATVR+S+ G E A NGPI AL A+ KAL E E +L +Y+V + +
Sbjct: 419 ATVRLSVEGVEE-HTASLGNGPISALDRALRKALEEFYPNLKELQLIDYKVRIINESAGT 477
Query: 458 LGEVLVRVE-VNGEIFVGR-GLSTDIIEAS 485
+V V +E +G+ G G+S ++IEAS
Sbjct: 478 SAKVRVLIESTDGKRKWGTVGVSENVIEAS 507
>gb|AAA22169.1| (M17349) nifV protein [Azotobacter vinelandii]
Length = 385
Score = 222 bits (560), Expect = 7e-57
Identities = 132/367 (35%), Positives = 199/367 (53%), Gaps = 12/367 (3%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
M V I DTTLRDGEQ+ GV+ +EK+ IA+ LA+L V +E G P E E + + I
Sbjct: 1 MASVIIDDTTLRDGEQSAGVAFNADEKIAIARALAELGVPELEIGIPSMGEEEREVMHAI 60
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL 120
A + A R + D+ A R+ + + S + + +KL ++ + +
Sbjct: 61 AGLGLSSRLLAWCRLCDVDL----AAARSTGVTMVDLSLPVSDLMLHHKLNRDRDWALRE 116
Query: 121 AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY 180
+ V A +V EDA+R+D +F+V+V E AGA + DTVG P
Sbjct: 117 VARLVGEARMAGLEVCLGCEDASRADLEFVVQVGEVAQAAGARPLRFADTVGVMEPFGML 176
Query: 181 ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL 240
+ R+L R + +++ VH HDD GLA AN+L+A+ GA + TVNG+GERAGNAAL
Sbjct: 177 DRFRFLSRRLD----MELEVHAHDDFGLATANTLAAVMGGATHINTTVNGLGERAGNAAL 232
Query: 241 EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ 300
EE ++AL ++ + + T ++ + I S LV +G +V K++VG F HE+GIH
Sbjct: 233 EECVLAL---KNLHGIDTGIDTRGIPAISALVERASGRQVAWQKSVVGAGVFTHEAGIHV 289
Query: 301 DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFK 360
DG+LK R YE ++P +LG S S +VLGKHSG H R +LG L + + + +
Sbjct: 290 DGLLKHRRNYEGLNPDELGRSHS-LVLGKHSGAHMVRNTYRDLGIELADWQSQALLGRIR 348
Query: 361 DIADRKR 367
+ R +
Sbjct: 349 AFSTRTK 355
>sp|P05342|NIFV_AZOVI HOMOCITRATE SYNTHASE >gi|420863|pir||S29758 nifV protein -
Azotobacter vinelandii >gi|142362|gb|AAA64727.1|
(M20568) homocitrate synthase (nifV) [Azotobacter
vinelandii]
Length = 385
Score = 222 bits (559), Expect = 9e-57
Identities = 132/367 (35%), Positives = 199/367 (53%), Gaps = 12/367 (3%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
M V I DTTLRDGEQ+ GV+ +EK+ IA+ LA+L V +E G P E E + + I
Sbjct: 1 MASVIIDDTTLRDGEQSAGVAFNADEKIAIARALAELGVPELEIGIPSMGEEEREVMHAI 60
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL 120
A + A R + D+ A R+ + + S + + +KL ++ + +
Sbjct: 61 AGLGLSSRLLAWCRLCDVDL----AAARSTGVTMVDLSLPVSDLMLHHKLNRDRDWALRE 116
Query: 121 AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY 180
+ V A +V EDA+R+D +F+V+V E AGA + DTVG P
Sbjct: 117 VARLVGEARMAGLEVCLGCEDASRADLEFVVQVGEVAQAAGARRLRFADTVGVMEPFGML 176
Query: 181 ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL 240
+ R+L R + +++ VH HDD GLA AN+L+A+ GA + TVNG+GERAGNAAL
Sbjct: 177 DRFRFLSRRLD----MELEVHAHDDFGLATANTLAAVMGGATHINTTVNGLGERAGNAAL 232
Query: 241 EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ 300
EE ++AL ++ + + T ++ + I S LV +G +V K++VG F HE+GIH
Sbjct: 233 EECVLAL---KNLHGIDTGIDTRGIPAISALVERASGRQVAWQKSVVGAGVFTHEAGIHV 289
Query: 301 DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFK 360
DG+LK R YE ++P +LG S S +VLGKHSG H R +LG L + + + +
Sbjct: 290 DGLLKHRRNYEGLNPDELGRSHS-LVLGKHSGAHMVRNTYRDLGIELADWQSQALLGRIR 348
Query: 361 DIADRKR 367
+ R +
Sbjct: 349 AFSTRTK 355
>emb|CAB91069.1| (AJ239033) homocitrate sythase 1 [Anabaena variabilis]
Length = 381
Score = 215 bits (541), Expect = 1e-54
Identities = 139/367 (37%), Positives = 196/367 (52%), Gaps = 12/367 (3%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
M KV I DTTLRDGEQ GV T+EEKV IAK L + V +E G P E E +A+ I
Sbjct: 1 MNKVLINDTTLRDGEQAAGVVFTLEEKVAIAKFLDTIGVPELEVGIPAMGEEEMRAICAI 60
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL 120
+ + A RAV DI +A R+H I S I + K + +
Sbjct: 61 SNLGLKANLLAWNRAVISDI----KASVACGMERVHIAIPVSGIQIAAKFHGQWRVSLQR 116
Query: 121 AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY 180
+ A V ED++R+D +FL++V + GA+ DTVG P Y
Sbjct: 117 LKDCISFAVDQGLWVAVGGEDSSRADENFLLDVALYAQEWGASRFRFCDTVGVLDPFTTY 176
Query: 181 ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL 240
V+ L ++ + + VH H+D G+A AN+L+ I+AGA V TV G+GERAGNAAL
Sbjct: 177 GKVKLLVSALT----IPVEVHTHNDFGMATANALAGIKAGAASVNTTVIGLGERAGNAAL 232
Query: 241 EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ 300
EEV++A+ + Y V ++ + S+LV+ +G VPP KAIVG N FAHESGIH
Sbjct: 233 EEVVMAI---KRIYGVDMGIDTPRLLELSQLVAAASGANVPPWKAIVGENTFAHESGIHA 289
Query: 301 DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFK 360
GVL+ TYE P+++G+ ++V+GKHSGRH+ LE+ G L E + +
Sbjct: 290 HGVLQNPDTYEPFAPEEVGWE-RRLVVGKHSGRHSVSNLLEQHGIFLNPEETQSVLDAVR 348
Query: 361 DIADRKR 367
+ +K+
Sbjct: 349 QQSVKKK 355
>gb|AAG27075.1|AF105225_11 (AF105225) NifV [Acetobacter diazotrophicus]
Length = 378
Score = 212 bits (534), Expect = 7e-54
Identities = 136/350 (38%), Positives = 185/350 (52%), Gaps = 19/350 (5%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
++ I DTTLRDGEQ PGV+ T EK+ IA L VD IEAG P + E + I
Sbjct: 6 RLLISDTTLRDGEQAPGVAFTAWEKLAIAGALDAAGVDEIEAGVPAMGDAEIAMIAAIGD 65
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
EVE + R ++D+ A R +H ++TS + A
Sbjct: 66 EVERARVIPWCRMRDEDV----HAARRTGLGSVHLSVSTSSGRSRRNTACRASHAGHGA- 120
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
+ V A Y V EDA+R+D +LV++ + +AGA DT+G P +E+
Sbjct: 121 RVVSRARDYGLAVSVGGEDASRADPAYLVDLLGVIAEAGAFRFRFADTLGVMDPFGVHEV 180
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
+RYL ++ +Q+ H HDDLGLA AN+L+A+RAGA VTV G+GERAGNA LEE
Sbjct: 181 MRYLCQS----SPLQLEFHGHDDLGLATANTLAAVRAGAACASVTVLGLGERAGNAPLEE 236
Query: 243 VIVALDVRRDFYKV---KTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIH 299
V+ + Y++ V L + + LVS ++PP+KAIVG F HESGIH
Sbjct: 237 VVAGV------YRLLGRPAGVKLDSLPGLATLVSRAAVRDIPPDKAIVGDAVFRHESGIH 290
Query: 300 QDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTE 349
G+L++ TYE +DP + G +IVLGKHSGR A R L LG E
Sbjct: 291 VSGLLRDAATYEALDPVQFG-RQREIVLGKHSGRAAVRHALAALGLDADE 339
>emb|CAB91068.1| (AJ239032) homocitrate synthase 2 [Anabaena variabilis]
Length = 379
Score = 208 bits (523), Expect = 1e-52
Identities = 131/333 (39%), Positives = 184/333 (54%), Gaps = 12/333 (3%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
M KV I DTTLRDGEQ GV+ +VEEK+ IAK L + VD IE G P + E +A+ I
Sbjct: 1 MNKVLINDTTLRDGEQAAGVAFSVEEKIAIAKFLDAIGVDEIEVGIPAMGKAEQEAIANI 60
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL 120
+ RAV DI +A R+H I S I + K + + V +
Sbjct: 61 VKLNLSANLLGWNRAVISDI----QASIACGLQRVHISIPVSAIQIAAKFHGQWQVVLQK 116
Query: 121 AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY 180
++ A V ED++R++ FL++V A + GA DTVG P +
Sbjct: 117 LHDSISFAVDQGLFVSIGGEDSSRAEESFLLDVVLAAQEWGALRFRFCDTVGILDPFTTH 176
Query: 181 ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL 240
++ L +++ + + +H H+D GLA AN+L+ I+AGA V TVNG+GERAGNAAL
Sbjct: 177 AKIKQLVASLT----IPVEMHTHNDFGLATANALAGIKAGALSVNTTVNGLGERAGNAAL 232
Query: 241 EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ 300
EEV++AL + Y ++ + + S+LV +G VPP KAIVG N FAHESGIH
Sbjct: 233 EEVVMAL---KHLYHHDLGIDTRRLLEISQLVVSASGHPVPPWKAIVGENTFAHESGIHA 289
Query: 301 DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGR 333
GVL+ TYE P+++G ++V+GKHSGR
Sbjct: 290 HGVLQNPQTYEPFAPEEVG-RERRLVVGKHSGR 321
>sp|P23122|NIFV_AZOCH HOMOCITRATE SYNTHASE >gi|280010|pir||C43706 nifV protein -
Azotobacter chroococcum >gi|142389|gb|AAA22161.1|
(M60090) homocitrate synthase [Azotobacter chroococcum]
Length = 382
Score = 205 bits (516), Expect = 9e-52
Identities = 130/367 (35%), Positives = 194/367 (52%), Gaps = 14/367 (3%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
M V I DTTLRDGEQ+ GV+ +EK+ I + LA+L V +E G P E E + ++ I
Sbjct: 1 MASVIIDDTTLRDGEQSAGVAFNADEKIAIRRALAELGVPELEIGIPSMGEEEREVMRAI 60
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL 120
A + A R + D+ A R+ + + S + +++KL ++ +
Sbjct: 61 AGLGLSSRLLAWCRLCDFDLSAA----RSTGVTMVDLSLPISDLMLRHKLNRDRDWALGE 116
Query: 121 AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY 180
+ V A +V EDA+R+D DF+V V AV A DTVG P
Sbjct: 117 VARLVSEARMAGLEVCLGCEDASRADQDFIVRV-GAVAQAARPPPAFADTVGVMEPFGML 175
Query: 181 ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL 240
+ R+LR+ + V++ VH HDD GLA AN+L+A+ GA + TVNG+GERA NAAL
Sbjct: 176 DRFRFLRQRLD----VELEVHAHDDFGLATANTLAAVMGGATHINTTVNGLGERAANAAL 231
Query: 241 EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ 300
EE ++AL ++ + + T ++ + I S LV +G + P +A + F HE+GIH
Sbjct: 232 EECVLAL---KNLHGIDTGIDTRGIPAISALVERASGRQWPGRRAWL-APVFTHEAGIHV 287
Query: 301 DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFK 360
DG+LK R YE ++P +LG S S +VLGKHSG H R ELG L + + + +
Sbjct: 288 DGLLKHRRNYEGLNPDELGRSHS-LVLGKHSGAHMVRNSYRELGIELADWQSQALLGRIR 346
Query: 361 DIADRKR 367
+ R +
Sbjct: 347 AFSTRTK 353
>pir||E75045 2-isopropylmalate synthase (leua-3) PAB0894 - Pyrococcus abyssi
(strain Orsay) >gi|5458771|emb|CAB50258.1| (AJ248287)
2-isopropylmalate synthase (leuA-3) [Pyrococcus abyssi]
Length = 526
Score = 204 bits (514), Expect = 2e-51
Identities = 159/510 (31%), Positives = 266/510 (51%), Gaps = 35/510 (6%)
Query: 2 RKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGE---FKAVK 58
R+V ++DTTLRDG Q G+S ++E+K++I ++L + + IE G+P ++ + FKAV+
Sbjct: 5 RRVELYDTTLRDGSQMEGISFSLEDKLKITEKLDEFGIHYIEGGWPGSNPKDIQYFKAVR 64
Query: 59 KIATEVEDPTIAALARA----VEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEP 114
+ +E+ IAA + + D AL +E F + +H+ LR
Sbjct: 65 DLP--LENAKIAAFGSTRRPRLRPEEDPNLNALVESEAPIATIFGKSWDLHVTEALRTTL 122
Query: 115 EEVKKLAVKAVEHATKYTEDVEFSAE---DATRSDWDFLVEVYEAVIDAGATVINVPDTV 171
E ++ + ++E+ + V + AE D R + ++ ++ +A +AGA I + DT
Sbjct: 123 ENNIQMIIDSIEYLRDHGMVVFYDAEHFFDGYRENPEYAMKTIKAAEEAGAERIVLADTN 182
Query: 172 GYATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGI 231
G + P E+V ++ + G+ H H+D LAVANSL A AG Q++ T+NG
Sbjct: 183 GGSLPSFIKEVVEKVKEEVKTPLGI----HAHNDSELAVANSLIAFEAGVVQIQGTINGY 238
Query: 232 GERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNA 291
GER GNA L +I AL+++ V + L+++ + V+ L +E+P N+ VG +A
Sbjct: 239 GERCGNANLISIIPALELKYGVEVVGKE-RLRKLKELAHFVAELANMEIPRNQPYVGDSA 297
Query: 292 FAHESGIHQDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEH 351
FAH+ G+H VLK TYE IDP+ +G + K+V+ + SGR K +ELG L E
Sbjct: 298 FAHKGGVHVSAVLKNPRTYEHIDPELVG-NRRKVVVSELSGRSNLIYKAKELGIELEERD 356
Query: 352 LERAFKKFKDIADRKRWI--TDTDIEAIIQEELTKSNGKLKVE----IIHVTSGK--VST 403
++R ++ K + + +E +I+ + ++E I + G+ +S
Sbjct: 357 IKRIVEEIKKLEFEGYHFEAAEASLELLIERLRGRYKPFFELERARVITEIFQGQPPISE 416
Query: 404 ATVRISMNGEERIEVAWFKNGPIDALFSAINKALG------EEFKLREYRVSSVTSGKDS 457
A V + +N R+ NGP++AL A+ KAL +E KL +Y+V + S K +
Sbjct: 417 ANVVVRVN-SRRVHTTAEGNGPVNALDLALRKALTKFYPELKEIKLVDYKVRVLGSEKGT 475
Query: 458 LGEVLVRVEV-NGEIFVGR-GLSTDIIEAS 485
+V V V+ +G+ G G ST+IIEAS
Sbjct: 476 AAKVRVLVQTSDGKKTWGTVGASTNIIEAS 505
>pir||B71181 probable 2-isopropylmalate synthase - Pyrococcus horikoshii
>gi|3258158|dbj|BAA30841.1| (AP000007) 361aa long
hypothetical 2-isopropylmalate synthase [Pyrococcus
horikoshii]
Length = 361
Score = 203 bits (510), Expect = 5e-51
Identities = 124/363 (34%), Positives = 198/363 (54%), Gaps = 15/363 (4%)
Query: 6 IFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVE 65
I D+TLR+GEQTPGV+ + E+++ IA L ++ VD IE G P S+ F +K IA++
Sbjct: 2 ILDSTLREGEQTPGVNYSPEQRLRIALALDEIGVDFIEVGHPAVSKDVFIGIKLIASQDL 61
Query: 66 DPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAV 125
+ + A +RA+ +DID + A+ + F S ++ + R + + KA+
Sbjct: 62 NANLLAHSRALLEDID----YVIQADVEWVGIFFCLSNACLRKRFRMSLSQALERISKAI 117
Query: 126 EHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRY 185
E+A + V F+ ED TR++W+ L E + I+V DT G P FY LV
Sbjct: 118 EYAKDHGLKVRFTPEDTTRTEWENLRRAIELAKELKVDRISVADTTGGTHPLRFYTLV-- 175
Query: 186 LRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIV 245
+ + N G+ ++VHCH+DLGLA+AN++ I GA V+ TVNG+GERAG L +++
Sbjct: 176 --KKVVNF-GIPVNVHCHNDLGLALANAIMGIEGGATVVDATVNGLGERAGIVDLAQIVT 232
Query: 246 ALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLK 305
L K + D ++ S++VS +TGI + PN IVG NAF H++G+H VLK
Sbjct: 233 VLYYHYGVKKYRLD----KLYEISRMVSEITGIALQPNYPIVGENAFTHKAGLHVSAVLK 288
Query: 306 ERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIADR 365
+ YE + + G + I + + +G+ R L++LG + EE ++ K+ K +
Sbjct: 289 DPRFYEFLPAEVFGRERT-IYVDRFAGKDTIRYYLQKLGIN-DEEFVKVLLKRVKSSREP 346
Query: 366 KRW 368
W
Sbjct: 347 FTW 349
>pir||G75159 2-isopropylmalate synthase (leua-2) PAB0286 - Pyrococcus abyssi
(strain Orsay) >gi|5457868|emb|CAB49358.1| (AJ248284)
2-isopropylmalate synthase (leuA-2) [Pyrococcus abyssi]
Length = 361
Score = 200 bits (504), Expect = 2e-50
Identities = 123/339 (36%), Positives = 186/339 (54%), Gaps = 14/339 (4%)
Query: 6 IFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVE 65
+ D+TLR+GEQTPGV+ T E+++EIA L ++ VD IE G P SE F+ +K IA +
Sbjct: 2 VLDSTLREGEQTPGVNYTPEQRLEIAIALDEVGVDFIEIGHPAVSEDVFRGMKLIAEQGL 61
Query: 66 DPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAV 125
+ + A +RA+ +DID + + + F S ++ + R + + +A+
Sbjct: 62 NVELLAHSRALIEDID----YVLKTGVDWVGIFFCLSEACLRKRFRITLDHALEKISRAI 117
Query: 126 EHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRY 185
E+A + V F+ ED TR++W L + I+V DT G P FY LV
Sbjct: 118 EYAKDHGLKVRFTPEDTTRTEWANLKRAIRLAKELKVDRISVADTTGSTHPLRFYTLV-- 175
Query: 186 LRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIV 245
+ I N G+ ++VHCH+DLGLA+AN++ I AGA V+ TVNG+GERAG L ++I
Sbjct: 176 --KKIVNF-GIPVNVHCHNDLGLALANAIMGIEAGATLVDATVNGLGERAGIVDLAQIIT 232
Query: 246 ALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLK 305
L K + D+ + R S LVS +TGI PN IVG NAF H++G+H VLK
Sbjct: 233 VLYYHYGIKKYRLDL----LYRVSNLVSEITGISPQPNYPIVGENAFTHKAGLHVSAVLK 288
Query: 306 ERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELG 344
+ YE + + G + I + +++G+ R LE+ G
Sbjct: 289 DPRFYEFLPAEVFGRERT-IYVDRYAGKDTIRYYLEKFG 326
>sp|Q01181|NIFV_RHOSH HOMOCITRATE SYNTHASE >gi|282178|pir||D41880 isopropylmalate
synthetase homolog NIfV - Rhodobacter sphaeroides
>gi|151969|gb|AAA26138.1| (M86823) nifV [Rhodobacter
sphaeroides]
Length = 391
Score = 200 bits (503), Expect = 3e-50
Identities = 137/378 (36%), Positives = 189/378 (49%), Gaps = 16/378 (4%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE 63
V + DTTLRDGEQT GV+ T EK IA+ L V +E G P E E ++ +A
Sbjct: 19 VALCDTTLRDGEQTAGVAFTRAEKRAIAEALQAAGVAEVEVGVPAMGEEERADIRAVAAV 78
Query: 64 VEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVK 123
++ R +D+ A + R+H + S + KL K+ V+ K
Sbjct: 79 LKTAAPVVWCRLRAEDL----AAAQRTGVVRLHIGVPVSERQISAKLGKDAAWVRDKVEK 134
Query: 124 AVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELV 183
V A+ V AEDA+R+D FL E+ +AGA + DT+G P +ELV
Sbjct: 135 LVRAASWAGHKVSVGAEDASRADPFFLAEIAHVAAEAGAIRFRISDTLGVLDPFAAHELV 194
Query: 184 RYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEV 243
+ + + H H+DLG+A ANSL+A RAGA + VTVNG+GERAGNAALEEV
Sbjct: 195 GRVVTRCP----LPVEFHGHNDLGMATANSLAAARAGASHLSVTVNGLGERAGNAALEEV 250
Query: 244 IVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGV 303
AL+ T V L ++ S+LV+ +G + P K IVG F HE GIH DG+
Sbjct: 251 AAALEAAGR----ATGVALGQLCALSELVARASGRPLSPQKPIVGEGVFTHECGIHVDGL 306
Query: 304 LKERTTYEIID--PKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKD 361
+K+R TYE D P++ G S +I +GKHS + L E G L +D
Sbjct: 307 MKDRATYESADLRPERFGRS-HRIAIGKHSSAAGLARALAEAGLPADAATLAALMPALRD 365
Query: 362 -IADRKRWITDTDIEAII 378
A KR D+ A++
Sbjct: 366 WAAITKRAAAPEDLAALL 383
>gb|AAK40481.1| 2-isopropylmalate synthase, putative (leuA-1) [Sulfolobus
solfataricus]
Length = 553
Score = 197 bits (496), Expect = 2e-49
Identities = 155/509 (30%), Positives = 259/509 (50%), Gaps = 38/509 (7%)
Query: 2 RKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIAS---EGEFKAVK 58
+ V + DTTLRDG Q +S T+ +K++IA L +L VD IE G+P ++ E F+ +K
Sbjct: 31 KSVEVLDTTLRDGSQGANISFTLNDKIKIALLLDELGVDYIEGGWPGSNPKDEEFFREIK 90
Query: 59 KIATEVEDPTIAALARAVEKDI----DRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEP 114
K + IAA KD+ D + ++ A+ + F + +H L+
Sbjct: 91 KY--RLSKAKIAAFGSTKRKDVSVKEDISLNSIVKADVDVAVIFGKSWSLHATEVLKVTK 148
Query: 115 EEVKKLAVKAVEHATKYTEDVEFSAE---DATRSDWDFLVEVYEAVIDAGATVINVPDTV 171
++ + ++ + + V F AE + D ++ +EV + AGA VI + DT
Sbjct: 149 QDNLDIVYDSINYLKSHGLKVIFDAEHFYQGFKEDPEYALEVVKTAESAGADVIALADTN 208
Query: 172 GYATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGI 231
G P E YE+ + +R + V++ +H H+D+G AVANSL AI+AGA V+ T+NGI
Sbjct: 209 GGTPPFEVYEITKKVREVLQ----VKLGIHAHNDIGCAVANSLMAIKAGARHVQGTINGI 264
Query: 232 GERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNA 291
GER GNA L ++I L ++ + +L+++ S++V + G+ P + VG NA
Sbjct: 265 GERTGNADLIQIIPTLILKMGLNALNGQESLRKLREVSRIVYEILGLPPNPYQPYVGDNA 324
Query: 292 FAHESGIHQDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSL--TE 349
FAH++G+H D V+K YE +DP +G + K V+ + SG L+ LG ++ +
Sbjct: 325 FAHKAGVHVDAVMKVPRAYEHVDPSLVG-NDRKFVISELSGTANLVSYLQGLGIAVDKKD 383
Query: 350 EHLERAFKKFKDIADRKRWITDTDIEAII--QEELTKSNGKLKVE---IIHVTSGKVSTA 404
E L++A K K++ R AI+ +EL + +E +I+ +G +S
Sbjct: 384 ERLKKALNKIKELEARGYSFDVGPASAILITLKELNIYKNYINLEYWKVINENNG-LSIG 442
Query: 405 TVRISMNGEERIEVAWFKNGPIDALFSAINKALGE------EFKLREYRVSSVTSGKDSL 458
V+++ ++EVA GP++A+ A+ AL E KL +YRV + K++
Sbjct: 443 IVKVN----SQLEVA-EGVGPVNAIDRALRMALQRVYPEIGEVKLIDYRVILPSEIKNTE 497
Query: 459 GEVLVRVEVNGEIFVGR--GLSTDIIEAS 485
V V +E R G+S ++EAS
Sbjct: 498 SVVRVTIEFTDNKMNWRTEGVSKSVVEAS 526
>sp|Q47884|NIFV_FRAAL HOMOCITRATE SYNTHASE >gi|1256029|gb|AAA96261.1| (L41344)
homocitrate synthase [Frankia alni]
Length = 351
Score = 195 bits (491), Expect = 8e-49
Identities = 123/337 (36%), Positives = 175/337 (51%), Gaps = 12/337 (3%)
Query: 8 DTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVEDP 67
DTTLRDGEQ PGV+ T EK+ IA L + V IEAG P E +++I
Sbjct: 27 DTTLRDGEQAPGVAFTAAEKLAIAAALDAIGVHQIEAGIPAMGVTERDVLREILATDPHA 86
Query: 68 TIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEH 127
I RA +D+ EA + H I S +H+K KL ++ + V
Sbjct: 87 DIVGWCRADHRDV----EAAASCGLVTAHLTIPVSDLHLKSKLGRDRAWARLRVRDCVAD 142
Query: 128 ATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRYLR 187
AT V EDA+R+D F+ ++ + + G T + DTVG P ++ + L
Sbjct: 143 ATDRGMRVSVGFEDASRADDAFVTDLAGELRELGVTRLRWADTVGLLDPVSAHDRLGRLV 202
Query: 188 RNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVAL 247
R + +H HDD GLA AN+++A++AG V TV G+GERAGNA +EEV +AL
Sbjct: 203 RAVPG----PWEIHAHDDFGLATANTIAAVQAGFTWVSTTVLGLGERAGNAPIEEVAMAL 258
Query: 248 DVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKER 307
R K+ D++ ++LVS +P KA+VG + FAHESGIH G+L+
Sbjct: 259 ---RHLLKLPVDLDTTSFRSLARLVSRAARRPLPAGKAVVGESVFAHESGIHVHGILRHP 315
Query: 308 TTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELG 344
TYE DP ++G ++ +GKHSGR + R LE+ G
Sbjct: 316 ATYEPFDPAEVG-GRRRLAIGKHSGRASVRYALEQYG 351
>sp|P54610|NIFV_FRASP HOMOCITRATE SYNTHASE >gi|1280032|gb|AAB36875.1| (U53363)
homocitrate synthase [Frankia sp.]
Length = 401
Score = 194 bits (487), Expect = 2e-48
Identities = 132/369 (35%), Positives = 188/369 (50%), Gaps = 18/369 (4%)
Query: 8 DTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVEDP 67
DTTLRDGEQ PGV+ T EK+ IA L + V IEAG P E +++I
Sbjct: 26 DTTLRDGEQAPGVAFTAAEKLAIAGALDAIGVHQIEAGIPGMGVTERDVLREILATDPKA 85
Query: 68 TIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEH 127
I RA +D+ EA + H I S +H+K KL ++ ++ V
Sbjct: 86 EIVGWCRADHRDV----EAAASCGLVTAHLTIPVSDLHLKSKLERDRAWARRRVRDCVVD 141
Query: 128 ATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVRYLR 187
T V EDA+R+D F+ ++ + D G T + DTVG P Y+ + L
Sbjct: 142 GTDRGMRVSVGFEDASRADDAFVTDLAGELRDVGVTRLRWADTVGLLDPVSAYDRLGRLV 201
Query: 188 RNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVAL 247
R + +H HDD GLA AN+++A++AG V TV G+GERAGNA +EEV +AL
Sbjct: 202 RAVPG----PWEIHAHDDFGLATANTIAAVQAGFTWVSTTVLGLGERAGNAPIEEVAMAL 257
Query: 248 DVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPP-NKAIVGGNAFAHESGIHQDGVLKE 306
R K+ D++ ++LV G +P KA+VG + FAHESGIH G+L+
Sbjct: 258 ---RHLLKLPIDLDTTSFRTLAQLV---VGWPLPAGKKAVVGESVFAHESGIHVHGILRH 311
Query: 307 RTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIADR- 365
TYE DP+ G ++ +GKHSGR + R LE+ G + E LE ++ + A R
Sbjct: 312 PATYEPFDPEVGG--RRRLTVGKHSGRASLRHALEQCGITAEESELEPLVEQVRLAATRH 369
Query: 366 KRWITDTDI 374
KR + D+
Sbjct: 370 KRGLDSRDL 378
>sp|Q07179|NIFV_RHOCA HOMOCITRATE SYNTHASE >gi|486695|pir||S34817 nifV protein -
Rhodobacter capsulatus >gi|297919|emb|CAA48488.1|
(X68444) nifV [Rhodobacter capsulatus]
Length = 382
Score = 188 bits (473), Expect = 1e-46
Identities = 128/356 (35%), Positives = 180/356 (49%), Gaps = 16/356 (4%)
Query: 2 RKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIA 61
R + + DTTLRDGEQT GV+ ++ EK IA+ L + V IE G E ++ +
Sbjct: 5 RTLALCDTTLRDGEQTAGVAFSLAEKKAIARALDRAGVAEIEVGIAAMGWAEVAEIRAVV 64
Query: 62 TEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLA 121
E+ T R D+D A + R+H + TS ++ KLR + + + +
Sbjct: 65 AEIAHATPVVWCRLRMHDLDMA----QKTGVKRVHFAVPTSTAQLEGKLRVDRDWILRET 120
Query: 122 VKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYE 181
V A+ V AEDA+R+D DFL+ + E AGA + DT+G P +
Sbjct: 121 AALVFCASDRGLQVSVGAEDASRTDPDFLIRLAEVAAAAGAIRFRIADTLGLLDPLGAFR 180
Query: 182 LVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALE 241
LV L IS + I +H H+D G+A AN++ A AGA + VTVNG+GERAGNAA E
Sbjct: 181 LVAELSARIS----LPIEMHAHNDFGMATANTIMAAHAGATHLSVTVNGLGERAGNAACE 236
Query: 242 EVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGN-AFAHESGIHQ 300
EV AL+ + T ++L + S +V+ +G K I G + FAHESGIH
Sbjct: 237 EVGAALEAG----GIDTGLDLCALPELSAVVAKASGRAFRAQKPITGDDWLFAHESGIHV 292
Query: 301 DGVLKERTTYE--IIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLER 354
D +LK TYE P + G +IV+GKHSG R L E G + L+R
Sbjct: 293 DAILKRADTYEDPRCAPARFG-RERQIVIGKHSGSAGLRAALLEAGLPADDAVLDR 347
>sp|Q9WZ22|LY41_THEMA PUTATIVE LYASE TM0552 >gi|7447065|pir||F72362 hypothetical protein
TM0552 - Thermotoga maritima (strain MSB8)
>gi|4981067|gb|AAD35637.1|AE001730_5 (AE001730)
2-isopropylmalate synthase, putative [Thermotoga
maritima]
Length = 538
Score = 182 bits (457), Expect = 8e-45
Identities = 146/519 (28%), Positives = 262/519 (50%), Gaps = 45/519 (8%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGE---FKAVKKI 60
+ ++DTTLRDG Q GVS ++E+K+ IA+ L L V +E G+P ++ + F+AVK +
Sbjct: 3 IKVYDTTLRDGAQAFGVSFSLEDKIRIAEALDDLGVHYLEGGWPGSNPKDIAFFEAVKGM 62
Query: 61 ATEVEDPTIAALARAVEKDI----DRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEE 116
++ +AA + D+ D + L AE F + +H++ LR EE
Sbjct: 63 --NFKNLKVAAFSSTRRPDVKIEEDANIQTLIKAETPVYTIFGKSWDLHVEKALRTTLEE 120
Query: 117 VKKLAVKAVEHATKYTEDVEFSAE---DATRSDWDFLVEVYEAVIDAGATVINVPDTVGY 173
K+ V + ++ ++V + AE D +++ ++ ++ + +AGA + + DT G
Sbjct: 121 NLKMIYDTVSYLKRFADEVIYDAEHFFDGYKANREYALKTLKVAEEAGADCLVLADTNGG 180
Query: 174 ATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGE 233
P E E++ +++++ + +H H+D +AVAN+L+A+R GA V+ T+NG+GE
Sbjct: 181 TLPHEIEEIIEDVKKHVK----APLGIHAHNDSDVAVANTLAAVRKGAVHVQGTINGLGE 236
Query: 234 RAGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFA 293
R GNA L VI L ++ + + NLK++ + LV+ L+G N VG AFA
Sbjct: 237 RCGNANLCSVIPNLVLKMGLEVIPKE-NLKKLFDVAHLVAELSGRPHIENMPYVGDYAFA 295
Query: 294 HESGIHQDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEE--H 351
H+ G+H + ++ TYE IDP+ +G + I + + SG+ +K++E+G+ + E
Sbjct: 296 HKGGVHVSAIKRDPRTYEHIDPELVG-NRRIISISELSGKSNVLEKIKEMGFEIDESSPK 354
Query: 352 LERAFKKFKDIADRKRWI--TDTDIEAIIQEELTKSN------GKLKVEIIHVTSGKVST 403
+ KK K++ + + E ++++ L K G + I + S
Sbjct: 355 VREILKKIKELEAQGYHFEGAEASFELLVRDMLGKRKKYFEFLGFTVMTIKNRDEESFSE 414
Query: 404 ATVRISMNGE--ERI-------EVAWFKNGPIDALFSAINKALG------EEFKLREY-- 446
ATV++ + E +R+ A GP++AL A+ KAL ++ KL +Y
Sbjct: 415 ATVKVRVPDEVAKRLGHDEPFEHTAAEGEGPVEALDRAVRKALEKFYPSLKDTKLTDYKV 474
Query: 447 RVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEAS 485
R+ + +G + VL+ + G+S +IIEAS
Sbjct: 475 RILNEQAGTKATTRVLIESSDGKRRWGTVGVSPNIIEAS 513
>gi|6320019 homocitrate synthase, highly homologous to YDL131W; Lys20p
[Saccharomyces cerevisiae]
>gi|1351812|sp|P48570|HOSC_YEAST HOMOCITRATE SYNTHASE,
CYTOSOLIC ISOZYME >gi|2131289|pir||S58735 homocitrate
synthase homolog YDL182w - yeast (Saccharomyces
cerevisiae) >gi|1004312|emb|CAA58264.1| (X83276) D1298
[Saccharomyces cerevisiae] >gi|1431295|emb|CAA98757.1|
(Z74230) ORF YDL182w [Saccharomyces cerevisiae]
Length = 428
Score = 181 bits (454), Expect = 2e-44
Identities = 118/377 (31%), Positives = 191/377 (50%), Gaps = 16/377 (4%)
Query: 6 IFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFK---AVKKIAT 62
+ D+TLR+GEQ E+K+EIA+ L VD IE P+ASE K A+ K+
Sbjct: 25 LIDSTLREGEQFANAFFDTEKKIEIARALDDFGVDYIELTSPVASEQSRKDCEAICKLGL 84
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
+ A + + +D A A+ + I TS +Y K+ + K AV
Sbjct: 85 K------AKILTHIRCHMDDAKVAVETGVDG-VDVVIGTSKFLRQYSHGKDMNYIAKSAV 137
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
+ +E ++ FS+ED+ RSD L+ +Y+ V G + + DTVG A P + YEL
Sbjct: 138 EVIEFVKSKGIEIRFSSEDSFRSDLVDLLNIYKTVDKIGVNRVGIADTVGCANPRQVYEL 197
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
+R L+ +S I H H+D G A+AN+ +A+ GA ++V+V GIGER G L
Sbjct: 198 IRTLKSVVS----CDIECHFHNDTGCAIANAYTALEGGARLIDVSVLGIGERNGITPLGG 253
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
++ + V Y VK+ L +I LV+ + +P N I G AF H++GIH
Sbjct: 254 LMARMIVAAPDY-VKSKYKLHKIRDIENLVADAVEVNIPFNNPITGFCAFTHKAGIHAKA 312
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDI 362
+L +TYEI+DP G + +G +A + ++++L +LT++ ++ K K +
Sbjct: 313 ILANPSTYEILDPHDFGMKRYIHFANRLTGWNAIKARVDQLNLNLTDDQIKEVTAKIKKL 372
Query: 363 ADRKRWITDTDIEAIIQ 379
D R + D+++II+
Sbjct: 373 GD-VRSLNIDDVDSIIK 388
>gi|6320071 homocitrate synthase, highly homologous to YDL182W; Lys21p
[Saccharomyces cerevisiae]
>gi|2492788|sp|Q12122|HOSM_YEAST HOMOCITRATE SYNTHASE,
MITOCHONDRIAL PRECURSOR >gi|2131288|pir||S67674
homocitrate synthase homolog YDL131w - yeast
(Saccharomyces cerevisiae) >gi|1419231|emb|CAA65629.1|
(X96876) putative ORF [Saccharomyces cerevisiae]
>gi|1431199|emb|CAA98700.1| (Z74179) ORF YDL131w
[Saccharomyces cerevisiae]
Length = 440
Score = 181 bits (454), Expect = 2e-44
Identities = 117/383 (30%), Positives = 194/383 (50%), Gaps = 16/383 (4%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFK---AV 57
++ + D+TLR+GEQ E+K+EIA+ L VD IE P+ASE K A+
Sbjct: 34 VKNFQLIDSTLREGEQFANAFFDTEKKIEIARALDDFGVDYIELTSPVASEQSRKDCEAI 93
Query: 58 KKIATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEV 117
K+ + A + + +D A A+ + I TS +Y K+ +
Sbjct: 94 CKLGLK------AKILTHIRCHMDDARVAVETGVDG-VDVVIGTSKFLRQYSHGKDMNYI 146
Query: 118 KKLAVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPE 177
K AV+ +E ++ FS+ED+ RSD L+ +Y+ V G + + DTVG A P
Sbjct: 147 AKSAVEVIEFVKSKGIEIRFSSEDSFRSDLVDLLNIYKTVDKIGVNRVGIADTVGCANPR 206
Query: 178 EFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGN 237
+ YEL+R L+ +S I H H+D G A+AN+ +A+ GA ++V+V GIGER G
Sbjct: 207 QVYELIRTLKSVVS----CDIECHFHNDTGCAIANAYTALEGGARLIDVSVLGIGERNGI 262
Query: 238 AALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESG 297
L ++ + V Y V++ L +I LV+ + +P N I G AF H++G
Sbjct: 263 TPLGGLMARMIVAAPDY-VRSKYKLHKIRDIENLVADAVEVNIPFNNPITGFCAFTHKAG 321
Query: 298 IHQDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFK 357
IH +L +TYEI+DP G + +G +A + ++++L +LT++ ++
Sbjct: 322 IHAKAILANPSTYEILDPHDFGMKRYIHFANRLTGWNAIKSRVDQLNLNLTDDQIKEVTA 381
Query: 358 KFKDIADRKRWITDTDIEAIIQE 380
K K + D R + D+++II++
Sbjct: 382 KIKKLGD-VRPLNIDDVDSIIKD 403
>sp|O87198|HOSC_THETH HOMOCITRATE SYNTHASE >gi|11268243|pir||T51170 homocitrate synthase
(EC 4.1.3.21) [validated] - Thermus aquaticus
>gi|3724367|dbj|BAA33785.1| (AB018379) homocitrate
synthase [Thermus thermophilus]
Length = 376
Score = 181 bits (454), Expect = 2e-44
Identities = 127/385 (32%), Positives = 195/385 (49%), Gaps = 12/385 (3%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
MR+ I D+TLR+GEQ + + ++KVEIAK L + ++ IE P+AS K + +
Sbjct: 1 MREWKIIDSTLREGEQFEKANFSTQDKVEIAKALDEFGIEYIEVTTPVASPQSRKDAEVL 60
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPE--EVK 118
A+ A + ++ +D A A+ + I TS R P E
Sbjct: 61 ASL---GLKAKVVTHIQCRLDAAKVAVETGVQG-IDLLFGTSKYLRAPHGRDIPRIIEEA 116
Query: 119 KLAVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEE 178
K + + A + E V FSAED RS+ L+ VYEAV + + DTVG ATP +
Sbjct: 117 KEVIAYIREAAPHVE-VRFSAEDTFRSEEQDLLAVYEAVAPY-VDRVGLADTVGVATPRQ 174
Query: 179 FYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNA 238
Y LVR +RR + V I H H+D G A+AN+ AI AGA V+ T+ GIGER G
Sbjct: 175 VYALVREVRRVVG--PRVDIEFHGHNDTGCAIANAYEAIEAGATHVDTTILGIGERNGIT 232
Query: 239 ALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGI 298
L + + + Y V+ L+ + ++V+ + G+E+P N I G AF+H++G+
Sbjct: 233 PLGGFLARMYTLQPEY-VRRKYKLEMLPELDRMVARMVGVEIPFNNYITGETAFSHKAGM 291
Query: 299 HQDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKK 358
H + YE P+ G I+ + +GRHA + + EELG EE L R +
Sbjct: 292 HLKAIYINPEAYEPYPPEVFGVKRKLIIASRLTGRHAIKARAEELGLHYGEEELHRVTQH 351
Query: 359 FKDIADRKRWITDTDIEAIIQEELT 383
K +ADR + +T +++ I++E +T
Sbjct: 352 IKALADRGQ-LTLEELDRILREWIT 375
>sp|O94225|HOSM_PENCH HOMOCITRATE SYNTHASE, MITOCHONDRIAL PRECURSOR
>gi|4165570|emb|CAA11503.1| (AJ223630) homocitrate
synthase [Penicillium chrysogenum]
Length = 474
Score = 177 bits (445), Expect = 2e-43
Identities = 118/377 (31%), Positives = 188/377 (49%), Gaps = 16/377 (4%)
Query: 6 IFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASE---GEFKAVKKIAT 62
I ++TLR+GEQ +K+EIAK L VD IE P ASE + +A+ K+
Sbjct: 69 IIESTLREGEQFANAFFDTAKKIEIAKALDDFGVDYIELTSPCASEQSRADCEAICKLGL 128
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
+ A + + +D A A+ + I TS ++ K+ +K A+
Sbjct: 129 K------AKILTHIRCHMDDARIAVETGVDG-VDVVIGTSSYLREHSHGKDMTYIKNAAI 181
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
+ +E ++ FS+ED+ RSD L+ +Y AV G + + DTVG A+P + YEL
Sbjct: 182 EVIEFVKSKGIEIRFSSEDSFRSDLVDLLSIYSAVDKVGVNRVGIADTVGCASPRQVYEL 241
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
VR LR + G I H H+D G A+AN+ A+ AGA ++ +V GIGER G L
Sbjct: 242 VRVLR----GVVGCDIETHFHNDTGCAIANAFCALEAGATHIDTSVLGIGERNGITPLGG 297
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
++ + V Y VK+ L+++ LV+ + +P N I G AF H++GIH
Sbjct: 298 LMARMMVADREY-VKSKYKLEKLKEIEDLVAEAVEVNIPFNNYITGFCAFTHKAGIHAKA 356
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDI 362
+L +TYEII+P G S + +G +A + + ++L +T+ + K K +
Sbjct: 357 ILNNPSTYEIINPADFGMSRYVHFASRLTGWNAIKSRAQQLKLEMTDTQYKECTAKIKAM 416
Query: 363 ADRKRWITDTDIEAIIQ 379
AD + D D ++II+
Sbjct: 417 ADIRPIAVD-DADSIIR 432
>sp|P74269|LY41_SYNY3 PUTATIVE LYASE SLL1564 >gi|7447061|pir||S75904 hypothetical protein
- Synechocystis sp. (strain PCC 6803)
>gi|1653449|dbj|BAA18363.1| (D90913)
alpha-isopropylmalate synthase [Synechocystis sp.]
Length = 547
Score = 176 bits (442), Expect = 4e-43
Identities = 146/518 (28%), Positives = 256/518 (49%), Gaps = 41/518 (7%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE 63
++++DTTLRDG Q G+SL++ +K+ IA++L +L + IE G+P A+ + + ++ E
Sbjct: 8 LWLYDTTLRDGAQREGISLSLTDKLTIARRLDQLGIPFIEGGWPGANPKDVQFFWQLQEE 67
Query: 64 -VEDPTIAALA------RAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEE 116
+E I A +AVE D + +A+ +A + F + +H+ L+ E
Sbjct: 68 PLEQAEIVAFCSTRRPHKAVETD--KMLQAILSAGTRWVTIFGKSWDLHVLEGLQTSLAE 125
Query: 117 VKKLAVKAVEHATKYTEDVEFSAE---DATRSDWDFLVEVYEAVIDAGATVINVPDTVGY 173
+ + + V + AE D R++ D+ + AGA + + DT G
Sbjct: 126 NLAMISDTIAYLRSQGRRVIYDAEHWFDGYRANPDYALATLATAQQAGAEWLVMCDTNGG 185
Query: 174 ATPEEFYELVRYLRRNIS----NIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVN 229
P + E+ +RR++ + + Q+ +H H+D G AVANSL A+ AGA V+ T+N
Sbjct: 186 TLPGQISEITTKVRRSLGLDGQSDRQPQLGIHAHNDSGTAVANSLLAVEAGATMVQGTIN 245
Query: 230 GIGERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGG 289
G GER GNA L +I L ++ D+ ++ + L + TS+L+S + + + VG
Sbjct: 246 GYGERCGNANLCTLIPNLQLKLDYDCIEPE-KLAHLTSTSRLISEIVNLAPDDHAPFVGR 304
Query: 290 NAFAHESGIHQDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTE 349
+AFAH+ GIH V + TYE I P +G + +IV+ + +G K E G +L
Sbjct: 305 SAFAHKGGIHVSAVQRNPFTYEHIAPNLVG-NERRIVVSEQAGLSNVLSKAELFGIALDR 363
Query: 350 EH--LERAFKKFKDIADR--KRWITDTDIEAIIQEELTKSNGKLKVEIIHV--------- 396
++ KD+ + + + E ++++ + V+ V
Sbjct: 364 QNPACRTILATLKDLEQQGYQFEAAEASFELLMRQAMGDRQPLFLVQGFQVHCDLLTPAE 423
Query: 397 -TSGKVSTATVRISMNGEERIEVAWFKNGPIDALFSAINKALGE------EFKLREYRVS 449
+ + + ATV++++NG+ +EVA NGP+ AL A+ KAL +F L +Y+V
Sbjct: 424 NPAYRNALATVKVTVNGQNILEVA-EGNGPVSALDQALRKALTRFYPQIADFHLTDYKVR 482
Query: 450 SVTSGKDSLGEVLVRVE-VNGE-IFVGRGLSTDIIEAS 485
+ G + + V VE NG+ + G+S +I+EAS
Sbjct: 483 ILDGGAGTSAKTRVLVESSNGDRRWTTVGVSGNILEAS 520
>sp|Q12726|HOSM_YARLI HOMOCITRATE SYNTHASE, MITOCHONDRIAL PRECURSOR
>gi|1246864|emb|CAA88928.1| (Z49114) homocitrate
synthase (acetyl-coenzyme A:2-Ketoglutarate
C-acetyltransferase) [Yarrowia lipolytica]
Length = 446
Score = 175 bits (438), Expect = 1e-42
Identities = 116/381 (30%), Positives = 192/381 (49%), Gaps = 16/381 (4%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASE---GEFKAVKK 59
K + ++TLR+GEQ E K+EIAK L VD IE P ASE + +A+ K
Sbjct: 47 KFQLIESTLREGEQFASAFFDTETKIEIAKALDDFGVDYIELTSPAASEQSRSDCEAICK 106
Query: 60 IATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKK 119
+ + A + + +D A A+ + I TS +Y K+ + +
Sbjct: 107 LGLK------AKILTHIRCHMDDARLAVSTGVDG-VDVVIGTSQFLRQYSHGKDMNYIAQ 159
Query: 120 LAVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEF 179
AV+ +E + ++ FS+ED+ RSD L+ +Y V G + + DTVG A P +
Sbjct: 160 SAVEVIEFVKSHGIEIRFSSEDSFRSDLVDLLNIYRTVDKIGVNRVGIADTVGCANPRQV 219
Query: 180 YELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAA 239
YELVR L+ +S I H H+D G A+AN+ +A+ AGA+ ++V+V GIGER G +
Sbjct: 220 YELVRTLKSVVS----CDIECHFHNDTGCAIANAYTALEAGANLIDVSVLGIGERNGITS 275
Query: 240 LEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIH 299
L ++ + + D V + L ++ LV+ + +P N I G AF H++GIH
Sbjct: 276 LGGLMARM-IAADRDYVLSKYKLHKLRDLENLVADAVQVNIPFNNPITGFCAFTHKAGIH 334
Query: 300 QDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKF 359
+L +TYEI++P G + + +G +A + ++++L LT+ + K
Sbjct: 335 AKAILANPSTYEILNPADFGLTRYIHFANRLTGWNAIKSRVDQLNLHLTDAQCKDVTAKI 394
Query: 360 KDIADRKRWITDTDIEAIIQE 380
K + D R + D+++II+E
Sbjct: 395 KKLGD-VRSLNIDDVDSIIRE 414
>pir||S02508 homocitrate synthase (EC 4.1.3.21) - Klebsiella pneumoniae
>gi|43832|emb|CAA31676.1| (X13303) nifV protein (AA
1-380) [Klebsiella pneumoniae]
Length = 380
Score = 174 bits (437), Expect = 2e-42
Identities = 119/381 (31%), Positives = 186/381 (48%), Gaps = 13/381 (3%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
M +V I DTTLRDGEQ+PGV+ EKV IA+ L + +E G P + E ++ +
Sbjct: 1 MERVLINDTTLRDGEQSPGVAFRTSEKVAIAEALYAAGITAMEVGTPAMGDEEIARIQLV 60
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL 120
++ D T+ R +I ++ + + + I S +YKLR+ + +
Sbjct: 61 RRQLPDATLMTWCRMNALEIRQSADL----GIDWVDISIPASDKLRQYKLREPLAVLLER 116
Query: 121 AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY 180
+ A V EDA+R+ L + E A + DTVG P
Sbjct: 117 LAMFIHLAHTLGLKVCIGCEDASRASGQTLRAIAEVAQQCAAARLRYADTVGLLDPFTTA 176
Query: 181 ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL 240
+ LR ++ +I +H H+DLG+A AN+L+A+ AGA V TV G+GERAGNAAL
Sbjct: 177 AQISALR----DVWSGEIEMHAHNDLGMATANTLAAVSAGATSVNTTVLGLGERAGNAAL 232
Query: 241 EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ 300
E V + L+ V+T V+ + + + V+ + P + +VG F HESG+H
Sbjct: 233 ETVALGLE---RCLGVETGVHFSALPASCQRVAEAAQRAIDPQQPLVGELVFTHESGVHV 289
Query: 301 DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFK 360
+L+ +Y+ I P +G S ++VLGKHSGR A +++GY L + + +
Sbjct: 290 AALLRHSESYQSIAPSLMGRS-YRLVLGKHSGRQAVNGVFDQMGYHLNAAQINQLLPAIR 348
Query: 361 DIADR-KRWITDTDIEAIIQE 380
A+ KR D ++ AI E
Sbjct: 349 RFAENWKRSPKDYELVAIYDE 369
>sp|Q52070|NIFV_ENTAG HOMOCITRATE SYNTHASE >gi|1480128|emb|CAA68021.1| (X99694) nifV
[Enterobacter agglomerans]
Length = 400
Score = 173 bits (434), Expect = 4e-42
Identities = 122/377 (32%), Positives = 186/377 (48%), Gaps = 13/377 (3%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE 63
V I DTTLRDGEQ+PGV+ EK+EIA+ L V +EAG P E E + + +
Sbjct: 18 VIINDTTLRDGEQSPGVAFRASEKLEIAQALVNAGVRELEAGTPALGEEECVRLAILRRQ 77
Query: 64 VEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVK 123
+ D + + R +I + + + + S + + KL + V
Sbjct: 78 LPDTVMMSWCRMNSAEIHDSARV----GMDWVDISVPASDLLREQKLHRPWPAVIASLKP 133
Query: 124 AVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELV 183
V+ A + V EDA+RS + L + E D G + DT+G P + +
Sbjct: 134 LVQLAHRLGMRVCMGCEDASRSADETLHMLAEVAADLGIERMRFADTLGILDPFSTFTRI 193
Query: 184 RYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEV 243
LR++ + +H H+DLG+A AN+L+A+RAGA V TV G+GERAGNAALE V
Sbjct: 194 SALRQHWHG----GLEIHAHNDLGMATANTLAAVRAGATHVNTTVLGLGERAGNAALESV 249
Query: 244 IVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGV 303
++LD + ++ E+ +LV+ G + +VGG F HESG+H +
Sbjct: 250 TLSLD---RCLHLDCGIDFTELPALCRLVAAAAGRTIDVQHPLVGGQVFTHESGLHVAAL 306
Query: 304 LKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIA 363
L++ +Y+ IDP +G +VLGKHSGR A LGY L+E ++ K A
Sbjct: 307 LRDPRSYQGIDPSLVG-REFNLVLGKHSGRQAVSGICARLGYILSETQTLLMLQEVKRFA 365
Query: 364 DR-KRWITDTDIEAIIQ 379
+ KR + ++ A+ Q
Sbjct: 366 EHFKRNPGEDEVIAMCQ 382
>pir||T39279 probable homocitrate synthase (EC 4.1.3.21), mitochondrial isozyme
precursor - fission yeast (Schizosaccharomyces pombe)
>gi|5531464|emb|CAB50965.1| (AL096851) probable
homocitrate synthase, mitochondrial isozyme precursor(ec
4.1.3.21) [Schizosaccharomyces pombe]
Length = 418
Score = 173 bits (434), Expect = 4e-42
Identities = 114/378 (30%), Positives = 189/378 (49%), Gaps = 16/378 (4%)
Query: 6 IFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEG---EFKAVKKIAT 62
I ++TLR+GEQ E+K++IAK L VD IE P+ASE + +A+ K+
Sbjct: 37 IIESTLREGEQFANAFFDTEKKIQIAKALDNFGVDYIELTSPVASEQSRQDCEAICKLGL 96
Query: 63 EVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV 122
+ + + + +D A A+ + I TS KY K+ + A
Sbjct: 97 KCK------ILTHIRCHMDDARVAVETGVDG-VDVVIGTSQYLRKYSHGKDMTYIIDSAT 149
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
+ + +V FS+ED+ RSD L+ +Y+AV G + + DTVG ATP + Y+L
Sbjct: 150 EVINFVKSKGIEVRFSSEDSFRSDLVDLLSLYKAVDKIGVNRVGIADTVGCATPRQVYDL 209
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
+R LR +S I H H+D G+A+AN+ A+ AGA ++ ++ GIGER G L
Sbjct: 210 IRTLRGVVS----CDIECHFHNDTGMAIANAYCALEAGATHIDTSILGIGERNGITPLGA 265
Query: 243 VIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDG 302
++ + V Y + L ++ LV+ +++P N I G AF H++GIH
Sbjct: 266 LLARMYVTDREY-ITHKYKLNQLRELENLVADAVEVQIPFNNYITGMCAFTHKAGIHAKA 324
Query: 303 VLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDI 362
+L +TYEI+ P+ G S V + +G +A + + E+L LT+ + + K +
Sbjct: 325 ILANPSTYEILKPEDFGMSRYVHVGSRLTGWNAIKSRAEQLNLHLTDAQAKELTVRIKKL 384
Query: 363 ADRKRWITDTDIEAIIQE 380
AD + D D++ +++E
Sbjct: 385 ADVRTLAMD-DVDRVLRE 401
>sp|P05345|NIFV_KLEPN HOMOCITRATE SYNTHASE >gi|479601|pir||S34848 homocitrate synthase
(EC 4.1.3.21) - Klebsiella pneumoniae
>gi|43881|emb|CAA31119.1| (X12600) nifV gene product (AA
1 - 381) [Klebsiella pneumoniae]
Length = 381
Score = 168 bits (420), Expect = 2e-40
Identities = 119/381 (31%), Positives = 187/381 (48%), Gaps = 12/381 (3%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
M +V I DTTLRDGEQ+PGV+ EKV IA+ L + +E G P + E ++ +
Sbjct: 1 MERVLINDTTLRDGEQSPGVAFRTSEKVAIAEALYAAGITAMEVGTPAMGDEEIARIQLV 60
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL 120
++ D T+ R +I ++ + + + I S +YKLR+ + +
Sbjct: 61 RRQLPDATLMTWCRMNALEIRQSADL----GIDWVDISIPASDKLRQYKLREPLAVLLER 116
Query: 121 AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY 180
+ A V EDA+R+ L + E +A A + DTVG P
Sbjct: 117 LAMFIHLAHTLGLKVCIGCEDASRASGQTLRAIAEVAQNAPAARLRYADTVGLLDPFTTA 176
Query: 181 ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL 240
+ LR ++ +I +H H+DLG+A AN+L+A+ AGA V TV G+GERAGNAA
Sbjct: 177 AQISALR----DVWSGEIEMHAHNDLGMATANTLAAVSAGATSVNTTVLGLGERAGNAAA 232
Query: 241 EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ 300
+ AL + R V+T V+ + + V+ + P + +VG F HESG+H
Sbjct: 233 WKP-SALGLER-CLGVETGVHFSALPALCQRVAEAAQRAIDPQQPLVGELVFTHESGVHV 290
Query: 301 DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFK 360
+L++ +Y+ I P +G S ++VLGKHSGR A +++GY L + + +
Sbjct: 291 AALLRDSESYQSIAPSLMGRS-YRLVLGKHSGRQAVNGVFDQMGYHLNAAQINQLLPAIR 349
Query: 361 DIADR-KRWITDTDIEAIIQE 380
A+ KR D ++ AI E
Sbjct: 350 RFAENWKRSPKDYELVAIYDE 370
>pir||S37297 nifV protein - Klebsiella pneumoniae >gi|149345|gb|AAA25157.1|
(M17350) nifV-encoded protein [Klebsiella pneumoniae]
Length = 381
Score = 166 bits (417), Expect = 4e-40
Identities = 119/381 (31%), Positives = 186/381 (48%), Gaps = 12/381 (3%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
M +V I DTTLRDGEQ+PGV+ EKV IA+ L + +E G P + E ++ +
Sbjct: 1 MERVLINDTTLRDGEQSPGVAFRTSEKVAIAEALYAAGITAMEVGTPAMGDEEIARIQLV 60
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKL 120
++ D T+ R +I ++ + + + I S +YKLR+ + +
Sbjct: 61 RRQLPDATLMTWCRMNALEIRQSADL----GIDWVDISIPASDKLPQYKLREPLAVLLER 116
Query: 121 AVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFY 180
+ A V EDA+R+ L + E +A A + DTVG P
Sbjct: 117 LAMFIHLAHTLGLKVCIGCEDASRASGQTLRAIAEVAQNAPAARLRYADTVGLLDPFTTA 176
Query: 181 ELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAAL 240
+ LR ++ +I +H H+DLG+A AN+L+A+ AGA V TV G+GERAGNAA
Sbjct: 177 AQISALR----DVWSGEIEMHAHNDLGMATANTLAAVSAGATSVNTTVLGLGERAGNAAA 232
Query: 241 EEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQ 300
+ AL + R V+T V+ + + V+ + P + +VG F HESG+H
Sbjct: 233 WKP-SALGLER-CLGVETGVHFSALPALCQRVAEAAQRAIDPQQPLVGELVFTHESGVHV 290
Query: 301 DGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFK 360
+L++ +Y+ I P +G S ++VLGKHSGR A +++GY L + + +
Sbjct: 291 AALLRDSESYQSIAPSLMGRS-YRLVLGKHSGRQAVNGVFDQMGYHLNAAQINQLLPAIR 349
Query: 361 DIADR-KRWITDTDIEAIIQE 380
A+ KR D + AI E
Sbjct: 350 RFAENWKRSPKDYQLVAIYDE 370
>sp|O31046|LEU1_STRCO 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE) >gi|2582531|gb|AAB82586.1|
(AF026444) 2-isopropylmalate synthase [Streptomyces
coelicolor]
Length = 573
Score = 165 bits (414), Expect = 9e-40
Identities = 146/532 (27%), Positives = 241/532 (44%), Gaps = 68/532 (12%)
Query: 9 TTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEV---- 64
T LRDG Q ++ K + L K+ VIE GFP + + +F V+ I E
Sbjct: 42 TDLRDGNQALIDPMSPVRKRAMFDLLVKMGYKVIEVGFPASGQTDFDFVRSIIEEPGAIP 101
Query: 65 EDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKA 124
+D TI+ L +A E I+R E+L+ A + +H F AT+P+ + R +++K++AV
Sbjct: 102 DDVTISVLTQAREDLIERTVESLKGARRATVHLFNATAPVFRRVVFRGSRDDIKQIAVDG 161
Query: 125 VEHATKYTEDV---------EFSAEDATRSDWDFLVEVYEAVIDA------GATVINVPD 169
+Y E + ++S E T ++ DF +EV EAV+D ++N+P
Sbjct: 162 TRLVMEYAEKLLGPETEFGYQYSPEIFTDTELDFALEVCEAVMDTYQPGPGREIILNLPA 221
Query: 170 TVGYATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVN 229
TV +TP + ++ RN+S + V +SVH H+D G AVA + A+ AGAD++E +
Sbjct: 222 TVERSTPSTHADRFEWMGRNLSRREHVCLSVHPHNDRGTAVAAAELALMAGADRIEGCLF 281
Query: 230 GIGERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGG 289
G GER GN L V L + V ++ +I + + +EV P VG
Sbjct: 282 GQGERTGNVDL----VTLGMNLFSQGVDPQIDFSDIDEIRRTWEYCNQMEVHPRHPYVGD 337
Query: 290 NAFAHESGIHQDGVLKE--------------------RTTYEIIDPKKLGFSGSKIV-LG 328
+ SG HQD + K Y IDPK +G S ++ +
Sbjct: 338 LVYTSFSGSHQDAIKKGFDAMEADAAARGVTVDDIEWAVPYLPIDPKDVGRSYEAVIRVN 397
Query: 329 KHSGRHAFRKKLEELGYSLTEEH---LERAFK-KFKDIADRKR-----WITDTDIEAIIQ 379
SG+ + Y L +H L R + +F + K IT T I + Q
Sbjct: 398 SQSGKGG-------IAYVLKNDHSLDLPRRMQIEFSKLIQAKTDAEGGEITPTAIWDVFQ 450
Query: 380 EELTKSN----GKLKVEIIHVTSGK--VSTATVRISMNGEERIEVAWFKNGPIDALFSAI 433
+E + G+++V T+ + V T TV +++G E V NGPI A F A+
Sbjct: 451 DEYLPNPDNPWGRIQVANGQTTTDRDGVDTLTVDATVDGAETTLVG-SGNGPISAFFHAL 509
Query: 434 NKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEAS 485
+ +G + +L +Y+ +++ G + + + ++ G G+ + AS
Sbjct: 510 -QGVGIDVRLLDYQEHTMSEGASAQAASYIECAIGDKVLWGIGIDANTTRAS 560
>sp|O86511|LY41_STRCO PUTATIVE LYASE SC1C2.10 >gi|7480336|pir||T29059 probable
2-isopropylmalate synthase (EC 4.1.3.12) - Streptomyces
coelicolor >gi|3355677|emb|CAA19977.1| (AL031124)
putative lyase [Streptomyces coelicolor A3(2)]
Length = 534
Score = 160 bits (401), Expect = 3e-38
Identities = 142/516 (27%), Positives = 243/516 (46%), Gaps = 49/516 (9%)
Query: 5 YIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEV 64
++FDTTLRDG Q G++LTV +K+ IA+ L V IE G+P A+ + + + E+
Sbjct: 12 HVFDTTLRDGAQREGINLTVADKLAIARHLDDFGVGFIEGGWPGANPRDTEFFARARQEI 71
Query: 65 E---------DPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPE 115
+ T A A A E D +AL ++ I + H++ LR +
Sbjct: 72 DFKHAQLVAFGSTRRAGANAAE---DHQVKALLDSGAQVITLVAKSHDRHVELALRTTLD 128
Query: 116 EVKKLAVKAVEHATKYTEDVEFSAE---DATRSDWDFLVEVYEAVIDAGATVINVPDTVG 172
E + V H V E D R++ ++ V +AGA V+ + DT G
Sbjct: 129 ENLAMVADTVSHLKAQGRRVFVDCEHFFDGYRANPEYAKSVVRTASEAGADVVVLCDTNG 188
Query: 173 YATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIG 232
P + +V + + G ++ +H DD G AVAN+L+A+ AGA V+ T NG G
Sbjct: 189 GMLPAQIQAVVATVLADT----GARLGIHAQDDTGCAVANTLAAVDAGATHVQCTANGYG 244
Query: 233 ERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAF 292
ER GNA L V+ AL+++ +V + L+E+ R S ++ + + ++ VG +AF
Sbjct: 245 ERVGNANLFPVVAALELKYG-KQVLPEGRLREMTRISHAIAEVVNLTPSTHQPYVGVSAF 303
Query: 293 AHESGIHQDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSL--TEE 350
AH++G+H + + Y+ IDP+ +G + ++++ +GR + K +ELG L E
Sbjct: 304 AHKAGLHASAIKVDPDLYQHIDPELVG-NTMRMLVSDMAGRASIELKGKELGIDLGGDRE 362
Query: 351 HLERAFKKFK--DIADRKRWITDTDIEAIIQEELTKSNGKLKVEIIHVTSGKVST----- 403
+ R ++ K ++A D E +++ E + G+ ++ V S + T
Sbjct: 363 LVGRVVERVKERELAGYTYEAADASFELLLRAE---AEGR-PLKYFEVESWRAITEDRPD 418
Query: 404 ------ATVRISMNGEERIEVAWFKNGPIDALFSAINKALGE------EFKLREYRVSSV 451
ATV++ G ERI NGP++AL ++ AL + + L +Y+V +
Sbjct: 419 GSHANEATVKLWAKG-ERIVATAEGNGPVNALDRSLRVALEKIYPELAKLDLVDYKVRIL 477
Query: 452 --TSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEAS 485
G S VL+ + G++ ++I AS
Sbjct: 478 EGVHGTQSTTRVLISTSDGTGEWATVGVAENVIAAS 513
>sp|Q44290|NIFV_ANASP HOMOCITRATE SYNTHASE >gi|1487879|emb|CAA68174.1| (X99902)
homocitrate synthase [Anabaena sp.]
Length = 379
Score = 154 bits (385), Expect = 2e-36
Identities = 119/360 (33%), Positives = 178/360 (49%), Gaps = 28/360 (7%)
Query: 1 MRKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKI 60
M KV I DTTLRDGEQ GV T+EEKV IAK L + V +E G P E E +A+ I
Sbjct: 1 MNKVLINDTTLRDGEQAAGVVFTLEEKVAIAKFLDTIGVPELEVGIPAMGEEEMRAICAI 60
Query: 61 ATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKY--KLRKEPEEVK 118
+ + A RAV DI +A R+H I S + K+ + R + +
Sbjct: 61 SNLGLKANLLAWNRAVISDI----KASVACGMERVHIAIPVSDVKAKFHGQWRVSLPKTQ 116
Query: 119 KLAVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEE 178
+L AV+ V ED++R+D +FL++V + GA+ DTVG P
Sbjct: 117 RLYHFAVDQGLW----VAVGGEDSSRADENFLLDVALYAQEWGASRFRFCDTVGVLDPFT 172
Query: 179 FYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNA 238
Y V+ L ++ + + VH H+D G+A N+L+ I+AGA V TV G+GERAGNA
Sbjct: 173 TYGKVKLLVSALT----IPVEVHTHNDFGMATLNALAGIKAGASSVNTTVIGLGERAGNA 228
Query: 239 ALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGI 298
ALEEV++A+ + Y V ++ + S+LV+ P + +G + + +
Sbjct: 229 ALEEVVMAI---KRIYGVDMGIDTPRLLELSQLVA-----AAPVLMSHLGRRSLV-KIPL 279
Query: 299 HQDGVLKERTTYEIIDPK-----KLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLE 353
+ V +I+ P K +++V+GKHSGRH+ LE+ G L E +
Sbjct: 280 LTNQVFMHMVYCKILIPMNHSHLKRSVGNARLVVGKHSGRHSVSNLLEQHGIFLNPEETQ 339
>pir||D75418 homocitrate synthase - Deinococcus radiodurans (strain R1)
>gi|6458984|gb|AAF10811.1|AE001972_1 (AE001972)
homocitrate synthase [Deinococcus radiodurans]
Length = 393
Score = 154 bits (385), Expect = 2e-36
Identities = 109/377 (28%), Positives = 179/377 (46%), Gaps = 10/377 (2%)
Query: 6 IFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVE 65
I D+TLR+GEQ + ++KVEIA+ L + IE P+ SE + ++K+
Sbjct: 21 IIDSTLREGEQFARGNFGTDDKVEIARALDAFGAEYIEVTTPMVSEQTRQDIRKLTGLGL 80
Query: 66 DPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAV 125
R +D+ RA + + + TS ++ K ++ A + +
Sbjct: 81 RAKFLTHVRCHMEDVQRAVDT----GVDGLDLLFGTSSFLREFSHGKSIAQIIDTAGEVI 136
Query: 126 EHATKYTEDVE--FSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELV 183
+ ++E FSAED RS+ L+ VY AV + G + + DTVG ATP + Y LV
Sbjct: 137 GWIKTHHPELEIRFSAEDTFRSEEADLMAVYSAVSELGVHRVGLADTVGVATPRQVYTLV 196
Query: 184 RYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEV 243
R +R+ I +G I H H+D G AV+N+ AI AGA ++ T+ GIGER G L +
Sbjct: 197 REVRKVIH--EGCGIEFHGHNDTGCAVSNAYEAIEAGATHIDTTILGIGERNGITPLGGL 254
Query: 244 IVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGV 303
+ + D + NL+ + ++++ + + VP N + G A+ H++G+H +
Sbjct: 255 LARM-FTFDPQGLIDKYNLELLPELDRMIARMVDLPVPWNNYLTGEFAYNHKAGMHLKAI 313
Query: 304 LKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIA 363
YE I P G K +G+HA K ELG E+ L R K +A
Sbjct: 314 YLNPGAYEAIPPGVFGVGRRIQAASKVTGKHAIAYKARELGLHYGEDALRRVTDHIKSLA 373
Query: 364 DRKRWITDTDIEAIIQE 380
++ + D +E +++E
Sbjct: 374 EQDE-LDDAHLEQVLRE 389
>emb|CAB69749.1| (AL137166) 2-ispoprylmalate synthase [Streptomyces coelicolor
A3(2)]
Length = 499
Score = 152 bits (380), Expect = 8e-36
Identities = 134/499 (26%), Positives = 227/499 (44%), Gaps = 68/499 (13%)
Query: 42 IEAGFPIASEGEFKAVKKIATEV----EDPTIAALARAVEKDIDRAGEALRNAEKNRIHT 97
+E GFP + + +F V+ I E +D TI+ L +A E I+R E+L+ A + +H
Sbjct: 1 MEVGFPASGQTDFDFVRSIIEEPGAIPDDVTISVLTQAREDLIERTVESLKGARRATVHL 60
Query: 98 FIATSPIHMKYKLRKEPEEVKKLAVKAVEHATKYTEDV---------EFSAEDATRSDWD 148
+ AT+P+ + R +++K++AV +Y E + ++S E T ++ D
Sbjct: 61 YNATAPVFRRVVFRGSRDDIKQIAVDGTRLVMEYAEKLLGPETEFGYQYSPEIFTDTELD 120
Query: 149 FLVEVYEAVIDA------GATVINVPDTVGYATPEEFYELVRYLRRNISNIKGVQISVHC 202
F +EV EAV+D ++N+P TV +TP + ++ RN+S + V +SVH
Sbjct: 121 FALEVCEAVMDTYQPGPGREIILNLPATVERSTPSTHADRFEWMGRNLSRREHVCLSVHP 180
Query: 203 HDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRDFYKVKTDVNL 262
H+D G AVA + A+ AGAD++E + G GER GN L V L + V ++
Sbjct: 181 HNDRGTAVAAAELALMAGADRIEGCLFGQGERTGNVDL----VTLGMNLFSQGVDPQIDF 236
Query: 263 KEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKE---------------- 306
+I + + +EV P VG + SG HQD + K
Sbjct: 237 SDIDEIRRTWEYCNQMEVHPRHPYVGDLVYTSFSGSHQDAIKKGFDAMEADAAARGVTVD 296
Query: 307 ----RTTYEIIDPKKLGFSGSKIV-LGKHSGRHAFRKKLEELGYSLTEEH---LERAFK- 357
Y IDPK +G S ++ + SG+ + Y L +H L R +
Sbjct: 297 DIEWAVPYLPIDPKDVGRSYEAVIRVNSQSGKGG-------IAYVLKNDHSLDLPRRMQI 349
Query: 358 KFKDIADRKR-----WITDTDIEAIIQEELTKSN----GKLKVEIIHVTSGK--VSTATV 406
+F + K IT T I + Q+E + G+++V T+ + V T TV
Sbjct: 350 EFSKLIQAKTDAEGGEITPTAIWDVFQDEYLPNPDNPWGRIQVANGQTTTDRDGVDTLTV 409
Query: 407 RISMNGEERIEVAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVE 466
+++G E V NGPI A F A+ + +G + +L +Y+ +++ G + +
Sbjct: 410 DATVDGAETTLVG-SGNGPISAFFHAL-QGVGIDVRLLDYQEHTMSEGASAQAASYIECA 467
Query: 467 VNGEIFVGRGLSTDIIEAS 485
+ ++ G G+ + AS
Sbjct: 468 IGDKVLWGIGIDANTTRAS 486
>gi|6324225 alpha-isopropylmalate synthase (2-Isopropylmalate Synthase); Leu4p
[Saccharomyces cerevisiae]
>gi|126192|sp|P06208|LEU1_YEAST 2-ISOPROPYLMALATE
SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE) (ALPHA-IPM
SYNTHETASE) >gi|82873|pir||A23872 2-isopropylmalate
synthase (EC 4.1.3.12) - yeast (Saccharomyces
cerevisiae) >gi|171841|gb|AAA34743.1| (M12893)
alpha-isopropylmalate synthase (EC 4.1.3.12) long form
[Saccharomyces cerevisiae] >gi|929849|emb|CAA90522.1|
(Z50161) 2-Isopropylmalate Synthase [Saccharomyces
cerevisiae] >gi|1302020|emb|CAA95980.1| (Z71380) ORF
YNL104c [Saccharomyces cerevisiae]
Length = 619
Score = 148 bits (371), Expect = 1e-34
Identities = 135/491 (27%), Positives = 218/491 (43%), Gaps = 49/491 (9%)
Query: 9 TTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVK-KIATEVEDP 67
T LRDG Q+ ++VE+K E +L + IE FP AS+ +F + + +D
Sbjct: 66 TDLRDGNQSLPDPMSVEQKKEYFHKLVNIGFKEIEVSFPSASQTDFDFTRYAVENAPDDV 125
Query: 68 TIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEH 127
+I L ++ E I R EAL A+K IHT++ATS + + EE AV+A +
Sbjct: 126 SIQCLVQSREHLIKRTVEALTGAKKATIHTYLATSDMFREIVFNMSREEAISKAVEATKL 185
Query: 128 ATKYTED----------VEFSAEDATRSDWDFLVEVYEAVIDA------GATVINVPDTV 171
K T+D EFS E + + +F VE+ EAV A + N+P TV
Sbjct: 186 VRKLTKDDPSQQATRWSYEFSPECFSDTPGEFAVEICEAVKKAWEPTEENPIIFNLPATV 245
Query: 172 GYATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGI 231
A+P + + + Y +I+ + V IS HCH+D G VA + + AGAD+VE + G
Sbjct: 246 EVASPNVYADQIEYFATHITEREKVCISTHCHNDRGCGVAATELGMLAGADRVEGCLFGN 305
Query: 232 GERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNA 291
GER GN L V VA+++ V +++ ++ +V I V G
Sbjct: 306 GERTGNVDL--VTVAMNMYTQ--GVSPNLDFSDLTSVLDVVERCNKIPVSQRAPYGGDLV 361
Query: 292 FAHESGIHQDGVLKE---------------RTTYEIIDPKKLGFSGSKIV-LGKHSGR-H 334
SG HQD + K R Y +DPK +G ++ + SG+
Sbjct: 362 VCAFSGSHQDAIKKGFNLQNKKRAQGETQWRIPYLPLDPKDIGRDYEAVIRVNSQSGKGG 421
Query: 335 AFRKKLEELGYSLTEEHLERAFKKFKDIADR-KRWITDTDIEAIIQEELTKSNGKLK-VE 392
A L LG L +D AD R + +I + +E ++ + + +
Sbjct: 422 AAWVILRSLGLDLPRNMQIEFSSAVQDHADSLGRELKSDEISKLFKEAYNYNDEQYQAIS 481
Query: 393 IIHV------TSGKVSTATVRISMNGEERIEVAWFKNGPIDALFSAINKALGEEFKLREY 446
+++ T +V T V++ G++ +++ NGPI +L A++ L F + Y
Sbjct: 482 LVNYNVEKFGTERRVFTGQVKV---GDQIVDIEGTGNGPISSLVDALSNLLNVRFAVANY 538
Query: 447 RVSSVTSGKDS 457
S+ SG +
Sbjct: 539 TEHSLGSGSST 549
>gb|AAA34744.1| (M12893) alpha-isopropylmalate synthase (EC 4.1.3.12) short form
[Saccharomyces cerevisiae]
Length = 589
Score = 148 bits (371), Expect = 1e-34
Identities = 135/491 (27%), Positives = 218/491 (43%), Gaps = 49/491 (9%)
Query: 9 TTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVK-KIATEVEDP 67
T LRDG Q+ ++VE+K E +L + IE FP AS+ +F + + +D
Sbjct: 36 TDLRDGNQSLPDPMSVEQKKEYFHKLVNIGFKEIEVSFPSASQTDFDFTRYAVENAPDDV 95
Query: 68 TIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEH 127
+I L ++ E I R EAL A+K IHT++ATS + + EE AV+A +
Sbjct: 96 SIQCLVQSREHLIKRTVEALTGAKKATIHTYLATSDMFREIVFNMSREEAISKAVEATKL 155
Query: 128 ATKYTED----------VEFSAEDATRSDWDFLVEVYEAVIDA------GATVINVPDTV 171
K T+D EFS E + + +F VE+ EAV A + N+P TV
Sbjct: 156 VRKLTKDDPSQQATRWSYEFSPECFSDTPGEFAVEICEAVKKAWEPTEENPIIFNLPATV 215
Query: 172 GYATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGI 231
A+P + + + Y +I+ + V IS HCH+D G VA + + AGAD+VE + G
Sbjct: 216 EVASPNVYADQIEYFATHITEREKVCISTHCHNDRGCGVAATELGMLAGADRVEGCLFGN 275
Query: 232 GERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNA 291
GER GN L V VA+++ V +++ ++ +V I V G
Sbjct: 276 GERTGNVDL--VTVAMNMYTQ--GVSPNLDFSDLTSVLDVVERCNKIPVSQRAPYGGDLV 331
Query: 292 FAHESGIHQDGVLKE---------------RTTYEIIDPKKLGFSGSKIV-LGKHSGR-H 334
SG HQD + K R Y +DPK +G ++ + SG+
Sbjct: 332 VCAFSGSHQDAIKKGFNLQNKKRAQGETQWRIPYLPLDPKDIGRDYEAVIRVNSQSGKGG 391
Query: 335 AFRKKLEELGYSLTEEHLERAFKKFKDIADR-KRWITDTDIEAIIQEELTKSNGKLK-VE 392
A L LG L +D AD R + +I + +E ++ + + +
Sbjct: 392 AAWVILRSLGLDLPRNMQIEFSSAVQDHADSLGRELKSDEISKLFKEAYNYNDEQYQAIS 451
Query: 393 IIHV------TSGKVSTATVRISMNGEERIEVAWFKNGPIDALFSAINKALGEEFKLREY 446
+++ T +V T V++ G++ +++ NGPI +L A++ L F + Y
Sbjct: 452 LVNYNVEKFGTERRVFTGQVKV---GDQIVDIEGTGNGPISSLVDALSNLLNVRFAVANY 508
Query: 447 RVSSVTSGKDS 457
S+ SG +
Sbjct: 509 TEHSLGSGSST 519
>sp|O59736|LEU1_SCHPO 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE) >gi|7489938|pir||T40500
2-isopropylmalate synthase - fission yeast
(Schizosaccharomyces pombe) >gi|3560136|emb|CAA20723.1|
(AL031534) 2-isopropylmalate synthase.
[Schizosaccharomyces pombe]
Length = 584
Score = 147 bits (367), Expect = 3e-34
Identities = 136/521 (26%), Positives = 220/521 (42%), Gaps = 49/521 (9%)
Query: 9 TTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEV---- 64
T LRDG Q + +EK+ K L + IE GFP AS+ +F V+ +
Sbjct: 50 TDLRDGNQALPDPMNGQEKLRYFKLLCSIGFKEIEVGFPSASQTDFAFVRHLIETPGLIP 109
Query: 65 EDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKA 124
+D TI+AL + E I R EALR A+ +H + A SP+ + R +E LA+K
Sbjct: 110 DDVTISALTPSREPLILRTIEALRGAKNATVHLYNACSPLFREVVFRNSKQETLDLAIKG 169
Query: 125 VEHATKYTEDV----------EFSAEDATRSDWDFLVEVYEAVID------AGATVINVP 168
+ T T++ E+S E + ++ DF +EV EAV A + N+P
Sbjct: 170 SKIVTAATKNALESKETNWGFEYSPETFSDTEPDFALEVCEAVKGMWKPSAAQPIIFNLP 229
Query: 169 DTVGYATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTV 228
TV +TP + +L+ Y NIS + V +S+H H+D G AVA + AG D++E +
Sbjct: 230 ATVEMSTPNTYADLIEYFSTNISEREKVCVSLHPHNDRGTAVAAAELGQLAGGDRIEGCL 289
Query: 229 NGIGERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVG 288
G GER GN L V L V +++ ++ ++ I V P G
Sbjct: 290 FGNGERTGNVDL----VTLAFNLYTQGVSPNLDFSKLDEIIRITEDCNKINVHPRHPYAG 345
Query: 289 GNAFAHESGIHQDGVLKERTTYE--------------IIDPKKLGFSGSKIV-LGKHSGR 333
F SG HQD + K Y+ +DP + + I+ + SG+
Sbjct: 346 NLVFTAFSGSHQDAISKGLKAYDERKAVDPVWKVPYLPLDPHDVNSEYAAIIRVNSQSGK 405
Query: 334 HAFRKKLE-ELGYSLTEEHLERAFKKFKDIADRK-RWITDTDIEAIIQEE-LTKSNGKLK 390
L+ G L KD +D K + ++ +I + + G+
Sbjct: 406 GGVAYLLKTNCGLDLPRALQVEFGSIVKDYSDTKGKELSIGEISDLFYTTYYLEFPGRFS 465
Query: 391 VEIIHVTSGKVSTATVR--ISMNGEE-----RIEVAWFKNGPIDALFSAINKALGEEFKL 443
V ++S + ++ + + GE+ R+ + NGP+ AL A+ + F +
Sbjct: 466 VNDYTLSSNGPQSKCIKCVVDIKGEKKDTPSRVVIEGVGNGPLSALVDALRRQFNISFDI 525
Query: 444 REYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEA 484
+Y ++ SG V + N F G G+ D+ A
Sbjct: 526 GQYSEHAIGSGNGVKAASYVEIIFNNTSFWGVGIDADVTSA 566
>sp|Q00853|NIVA_CLOPA HOMOCITRATE SYNTHASE, ALPHA SUBUNIT >gi|98609|pir||B39403 probable
homocitrate synthase (EC 4.1.3.21) NifV omega chain -
Clostridium pasteurianum >gi|144879|gb|AAA23268.1|
(M68878) nitrogen fixation protein [Clostridium
pasteurianum]
Length = 269
Score = 147 bits (367), Expect = 3e-34
Identities = 85/234 (36%), Positives = 133/234 (56%), Gaps = 8/234 (3%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE 63
+ I DTTLRDGEQ G++L+V++KVEIAK ++++ V IEAG P E +V KIA
Sbjct: 3 INIVDTTLRDGEQKAGIALSVQDKVEIAKIISEMGVHQIEAGIPAMGGDEKISVSKIAAL 62
Query: 64 VEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVK 123
IAA R KDID + E + +H S + +K KL K+ + V + +
Sbjct: 63 GLPSKIAAWNRMSTKDIDTSIE----CGVDIVHISSPVSDLQIKTKLEKDRKWVAENLKR 118
Query: 124 AVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELV 183
V +A + +V ED++R+D +FL+++ E + G + DTVG P+E Y +
Sbjct: 119 TVIYALEKDCEVTVGLEDSSRADLNFLIQLCEMIFALGVKRVRYADTVGIMEPKELYSQI 178
Query: 184 RYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGN 237
+ +R + + I +H H+D G+A++NS +A +AGA + T+ G+GERAGN
Sbjct: 179 KKIRDKVP----IDIEIHVHNDFGMAISNSFAAFKAGAKFADCTITGMGERAGN 228
>sp|Q9X7L2|LEU1_RHIME 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE)
Length = 569
Score = 147 bits (367), Expect = 3e-34
Identities = 132/510 (25%), Positives = 232/510 (44%), Gaps = 40/510 (7%)
Query: 11 LRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE---VEDP 67
LRDG Q + + K + L + IE GFP AS+ ++ + E ED
Sbjct: 48 LRDGNQALVDPMGHDRKARMFHLLLDMGFKEIEIGFPSASQTDYDFARWCVEEGNVSEDV 107
Query: 68 TIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEH 127
++ L + + I R EAL A + +H + +TS + + K+ +K++A A +
Sbjct: 108 SLQVLVQCRPELIARTFEALEGAHRPIVHFYNSTSELQRRVVFGKDVAGIKQIATDAAKM 167
Query: 128 ATKYTEDV------EFSAEDATRSDWDFLVEVYEAVIDA------GATVINVPDTVGYAT 175
T E+S E T ++ + +E+ AVI+ +IN+P TV AT
Sbjct: 168 ITDMAAKAGGGYRFEYSPESFTGTELEVALEICNAVIEIVRPTADNKLIINLPSTVEMAT 227
Query: 176 PEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERA 235
P + + + ++ RN+ N + + +S+H H+D G +A + + AGAD+VE T+ G GER
Sbjct: 228 PNIYADQIEWMCRNLDNRENLIVSLHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERT 287
Query: 236 GNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHE 295
GN ++ V +AL++ K D + +I R ++ + + +P VG +
Sbjct: 288 GN--VDVVTLALNMFTQGVDPKLDCS--DIERIKEVYEYSNQMVIPERHPYVGELVYTAF 343
Query: 296 SGIHQDGVLK--------ERTTYEI----IDPKKLGFSGSKIV-LGKHSGRHAFRKKL-E 341
SG HQD + K + T+E+ IDP+ +G S I+ + SG+ L E
Sbjct: 344 SGSHQDAINKGMKAIKQANKPTWEVPYLPIDPRDVGRSYEAIIRINSQSGKGGIAYILQE 403
Query: 342 ELGYSLTEEHLERAFKKFKDIADR--KRWITDTDIEAIIQEELTKSNGKLKVEIIHV-TS 398
+ G +L ++ + I D K + + I+ + + ++K H +
Sbjct: 404 DYGINLPRNLQIEFREEVQRITDEEGKELPSKRIHQRFIESYVEQPGARIKFVDHHTYPA 463
Query: 399 GK---VSTATVRISMNGEERIEVAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGK 455
G+ + I+ GE R ++ GPID +A++ LG E + +Y S+ G
Sbjct: 464 GEHKGLRVVAAEITDGGETR-QIEGKGTGPIDGFINALSIYLGIELSVADYSEHSLQHGS 522
Query: 456 DSLGEVLVRVEVNGEIFVGRGLSTDIIEAS 485
++ V VE G G G++T+I+ AS
Sbjct: 523 NAAAIAYVEVEYPGGKLFGVGINTNIVAAS 552
>emb|CAB39977.1| (AJ132004) 2-isopropylmalate synthase [Sinorhizobium meliloti]
Length = 569
Score = 146 bits (366), Expect = 4e-34
Identities = 132/510 (25%), Positives = 232/510 (44%), Gaps = 40/510 (7%)
Query: 11 LRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE---VEDP 67
LRDG Q + + K + L + IE GFP AS+ ++ + E ED
Sbjct: 48 LRDGNQALVDPMGHDRKARMFHLLLDMGFKEIEIGFPSASQTDYDFARWCVEEGNVSEDV 107
Query: 68 TIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEH 127
++ L + + I R EAL A + +H + +TS + + K+ +K++A A +
Sbjct: 108 SLQVLVQCRPELIARTFEALEGAHRPIVHFYNSTSELQRRVVFGKDVAGIKQIATDAAKM 167
Query: 128 ATKYTEDV------EFSAEDATRSDWDFLVEVYEAVIDA------GATVINVPDTVGYAT 175
T E+S E T ++ + +E+ AVI+ +IN+P TV AT
Sbjct: 168 ITDMAAKAGGGYRFEYSPESFTGTELEVALEICNAVIEIVRPTADNKLIINLPSTVEMAT 227
Query: 176 PEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERA 235
P + + + ++ RN+ N + + +S+H H+D G +A + + AGAD+VE T+ G GER
Sbjct: 228 PNIYADQIEWMCRNLDNRENLIVSLHPHNDRGTGIAATDLGLMAGADRVEGTLFGNGERT 287
Query: 236 GNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHE 295
GN ++ V +AL++ K D + +I R ++ + + +P VG +
Sbjct: 288 GN--VDVVTLALNMFTQGVDPKLDCS--DIERIKEVYEYSNQMVIPERHPYVGELVYTAF 343
Query: 296 SGIHQDGVLK--------ERTTYEI----IDPKKLGFSGSKIV-LGKHSGRHAFRKKL-E 341
SG HQD + K + T+E+ IDP+ +G S I+ + SG+ L E
Sbjct: 344 SGSHQDAINKGMKAIKQANKPTWEVPYLPIDPRDVGRSYEAIIRINSQSGKGGIAYILQE 403
Query: 342 ELGYSLTEEHLERAFKKFKDIADR--KRWITDTDIEAIIQEELTKSNGKLKVEIIHV-TS 398
+ G +L ++ + I D K + + I+ + + ++K H +
Sbjct: 404 DYGINLPRNLQIEFREEVQRITDEEGKELPSKRIHQRFIESYVEQPGARIKFVDHHTYPA 463
Query: 399 GK---VSTATVRISMNGEERIEVAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGK 455
G+ + I+ GE R ++ GPID +A++ LG E + +Y S+ G
Sbjct: 464 GEHKGLRVVAAEITDGGETR-QIEGKGTGPIDGFINALSIYLGIELSVADYSEHSLQHGS 522
Query: 456 DSLGEVLVRVEVNGEIFVGRGLSTDIIEAS 485
++ V VE G G G++T+I+ AS
Sbjct: 523 NAAAIAYVEVEYPGGKLFGVGINTNIVAAS 552
>gi|6324682 Yor108wp [Saccharomyces cerevisiae] >gi|2132057|pir||S66993
hypothetical protein YOR108w - yeast (Saccharomyces
cerevisiae) >gi|1262141|emb|CAA64028.1| (X94335)
YOR3227w [Saccharomyces cerevisiae]
>gi|1420293|emb|CAA99306.1| (Z75016) ORF YOR108w
[Saccharomyces cerevisiae]
Length = 604
Score = 146 bits (364), Expect = 6e-34
Identities = 138/542 (25%), Positives = 233/542 (42%), Gaps = 88/542 (16%)
Query: 9 TTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVK-KIATEVEDP 67
T LRDG Q+ ++V +K E +L + IE FP AS+ +F + + +D
Sbjct: 65 TDLRDGNQSLPDPMSVAQKKEYFHKLINIGFKEIEVSFPSASQTDFDFTRYAVENAPDDV 124
Query: 68 TIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEH 127
I L ++ E I R EAL A++ IHT++ATS + + EE AV+A +
Sbjct: 125 GIQCLVQSREHLIKRTVEALTGAKRATIHTYLATSDMFREIVFNMSREEAISKAVEATKL 184
Query: 128 ATKYTED----------VEFSAEDATRSDWDFLVEVYEAVIDA------GATVINVPDTV 171
K T+D EFS E + + +F VE+ EAV A + N+P TV
Sbjct: 185 VRKLTKDDPSQQATRWSYEFSPECFSDTPGEFAVEICEAVKKAWEPTEENPIIFNLPATV 244
Query: 172 GYATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGI 231
A+P + + + Y +I+ + V IS HCH+D G VA + + AGAD+VE + G
Sbjct: 245 EVASPNVYADQIEYFSTHITEREKVCISTHCHNDRGCGVAATELGMLAGADRVEGCLFGN 304
Query: 232 GERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNA 291
GER GN L V VA+++ V +++ ++ S++V I +PP G
Sbjct: 305 GERTGNVDL--VTVAMNMYTQ--GVSPNLDFSDLTSISEIVHRCNKIPIPPRAPYGGELV 360
Query: 292 FAHESGIHQDGVLKE---------------RTTYEIIDPKKLGFSGSKIV-LGKHSGR-- 333
+ SG HQD + K R Y +DPK +G ++ + SG+
Sbjct: 361 VSAFSGSHQDAIKKGFAIQNKKQAQGETRWRIPYLPLDPKDIGRDYEAVIRVNSQSGKGG 420
Query: 334 ----------------------HAFRKKLEELGYSLTEEHLERAFKKFKDIADRKR-WIT 370
+ +K + LG L E + + FK+ + + + ++T
Sbjct: 421 AAWVIMRSLGLDVPRPMQVDFSNTLQKNADALGRELKSEEITKLFKETYNYNNNEHIYVT 480
Query: 371 DTDIEAIIQEELTKSNGKLKVEIIHVTSGKVSTATVRISMNGEERIEVAWFKNGPIDALF 430
++ E+ K N + + + G+V ++ + + + NGPI +L
Sbjct: 481 ------LLNYEVKKLNPERRALV-----GQVEI--------NDKVVNIEGYGNGPISSLV 521
Query: 431 SAINKALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEI-------FVGRGLSTDIIE 483
A++ L + ++ Y S+ SG + + + +I G G+S D +
Sbjct: 522 DALSNLLNVKLSVQNYSEHSLGSGSATQAASFINLSYIKDINNHATSNMWGVGVSEDTGD 581
Query: 484 AS 485
AS
Sbjct: 582 AS 583
>pir||I40725 2-isopropylmalate synthase (EC 4.1.3.12) - Corynebacterium
glutamicum >gi|452382|emb|CAA50295.1| (X70959)
2-isopropylmalate synthase [Corynebacterium glutamicum]
Length = 638
Score = 145 bits (361), Expect = 1e-33
Identities = 140/531 (26%), Positives = 238/531 (44%), Gaps = 68/531 (12%)
Query: 11 LRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE---VEDP 67
LRDG Q ++ E K + + L ++ IE GFP AS+ +F V++I + +D
Sbjct: 96 LRDGNQALIDPMSPERKRRMFELLVQMGFKEIEVGFPSASQTDFDFVREIIEKGMIPDDV 155
Query: 68 TIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEH 127
TI L +A E I R EA A+ +H + +TS + R + +VKKLA A E
Sbjct: 156 TIQVLVQAREHLIRRTFEACEGAKNVIVHFYNSTSILQRNVVFRMDKVQVKKLATDAAEL 215
Query: 128 ATKYTEDV-------EFSAEDATRSDWDFLVEVYEAVIDA------GATVINVPDTVGYA 174
+D ++S E T ++ ++ EV +AV++ +IN+P TV
Sbjct: 216 IKTIAQDYPDTNWRWQYSPESFTGTEVEYAKEVVDAVVEVMDPTPENPMIINLPSTVEMI 275
Query: 175 TPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGER 234
TP + + + ++ RN++ + +S+H H+D G V + AGAD++E + G GER
Sbjct: 276 TPNVYADSIEWMHRNLNRRDSIILSLHPHNDRGTGVGAAELGYMAGADRIEGCLFGNGER 335
Query: 235 AGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAH 294
GN L V +AL++ V ++ +I + V + + VP G F
Sbjct: 336 TGNVCL--VTLALNMLTQ--GVDPQLDFTDIRQIRSTVEYCNQLRVPERHPYGGDLVFTA 391
Query: 295 ESGIHQDGVLK-----------------------ERTTYEI----IDPKKLGFSGSKIV- 326
SG HQD V K T +E+ IDPK +G ++
Sbjct: 392 FSGSHQDAVNKGLDAMAAKVQPGASSTEVSWEQLRDTEWEVPYLPIDPKDVGRDYEAVIR 451
Query: 327 LGKHSGRH--AFRKKLE---------ELGYSLTEEHLERAFKKFKDIADRKRWITDTDIE 375
+ SG+ A+ K + ++ +S +++ A + ++ + W DI
Sbjct: 452 VNSQSGKGGVAYIMKTDHGLQIPRSMQVEFSTVVQNVTDA--EGGEVNSKAMW----DIF 505
Query: 376 AIIQEELTKSNGKLKVEIIHV-TSGKVSTATVRISMNGEERIEVAWFKNGPIDALFSAIN 434
A E T ++ + + + T + ++ T + NG++ + V NGP+ A +A+
Sbjct: 506 ATEYLERTAPVEQIALRVENAQTENEDASITAELIHNGKD-VTVDGRGNGPLAAYANALE 564
Query: 435 KALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEAS 485
K LG + +++EY + TSG D+ V EVNG G G++ I AS
Sbjct: 565 K-LGIDVEIQEYNQHARTSGDDAEAAAYVLAEVNGRKVWGVGIAGSITYAS 614
>sp|P42455|LEU1_CORGL 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE)
Length = 616
Score = 145 bits (361), Expect = 1e-33
Identities = 140/531 (26%), Positives = 238/531 (44%), Gaps = 68/531 (12%)
Query: 11 LRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE---VEDP 67
LRDG Q ++ E K + + L ++ IE GFP AS+ +F V++I + +D
Sbjct: 74 LRDGNQALIDPMSPERKRRMFELLVQMGFKEIEVGFPSASQTDFDFVREIIEKGMIPDDV 133
Query: 68 TIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEH 127
TI L +A E I R EA A+ +H + +TS + R + +VKKLA A E
Sbjct: 134 TIQVLVQAREHLIRRTFEACEGAKNVIVHFYNSTSILQRNVVFRMDKVQVKKLATDAAEL 193
Query: 128 ATKYTEDV-------EFSAEDATRSDWDFLVEVYEAVIDA------GATVINVPDTVGYA 174
+D ++S E T ++ ++ EV +AV++ +IN+P TV
Sbjct: 194 IKTIAQDYPDTNWRWQYSPESFTGTEVEYAKEVVDAVVEVMDPTPENPMIINLPSTVEMI 253
Query: 175 TPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGER 234
TP + + + ++ RN++ + +S+H H+D G V + AGAD++E + G GER
Sbjct: 254 TPNVYADSIEWMHRNLNRRDSIILSLHPHNDRGTGVGAAELGYMAGADRIEGCLFGNGER 313
Query: 235 AGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAH 294
GN L V +AL++ V ++ +I + V + + VP G F
Sbjct: 314 TGNVCL--VTLALNMLTQ--GVDPQLDFTDIRQIRSTVEYCNQLRVPERHPYGGDLVFTA 369
Query: 295 ESGIHQDGVLK-----------------------ERTTYEI----IDPKKLGFSGSKIV- 326
SG HQD V K T +E+ IDPK +G ++
Sbjct: 370 FSGSHQDAVNKGLDAMAAKVQPGASSTEVSWEQLRDTEWEVPYLPIDPKDVGRDYEAVIR 429
Query: 327 LGKHSGRH--AFRKKLE---------ELGYSLTEEHLERAFKKFKDIADRKRWITDTDIE 375
+ SG+ A+ K + ++ +S +++ A + ++ + W DI
Sbjct: 430 VNSQSGKGGVAYIMKTDHGLQIPRSMQVEFSTVVQNVTDA--EGGEVNSKAMW----DIF 483
Query: 376 AIIQEELTKSNGKLKVEIIHV-TSGKVSTATVRISMNGEERIEVAWFKNGPIDALFSAIN 434
A E T ++ + + + T + ++ T + NG++ + V NGP+ A +A+
Sbjct: 484 ATEYLERTAPVEQIALRVENAQTENEDASITAELIHNGKD-VTVDGRGNGPLAAYANALE 542
Query: 435 KALGEEFKLREYRVSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEAS 485
K LG + +++EY + TSG D+ V EVNG G G++ I AS
Sbjct: 543 K-LGIDVEIQEYNQHARTSGDDAEAAAYVLAEVNGRKVWGVGIAGSITYAS 592
>pir||C83494 probable 2-isopropylmalate synthase PA1217 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9947145|gb|AAG04606.1|AE004551_10 (AE004551)
probable 2-isopropylmalate synthase [Pseudomonas
aeruginosa]
Length = 455
Score = 145 bits (361), Expect = 1e-33
Identities = 108/351 (30%), Positives = 170/351 (47%), Gaps = 25/351 (7%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE 63
+ + D TLR+G Q S + V++A+++A L VD+IE G P S E + V+ +
Sbjct: 2 IRVIDCTLREGCQARQCSFDSAQSVQLAREIAALGVDMIECGHPRISADERERVRAVVAA 61
Query: 64 VEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLR-KEPEEVKKLAV 122
V P +A ARA +DID A+ + F + + I ++ K + EEV +L
Sbjct: 62 VSTPVLAH-ARARREDID----AVLETGAQWVGLFASINAISLETKFKGMNREEVLELFH 116
Query: 123 KAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYEL 182
A+ +A + V + EDA R+ LV + DAGA I D+VG P+E +++
Sbjct: 117 DAIRYARERGLRVRATVEDAARTSVSDLVSMIATARDAGADRICFADSVGILLPDETFDV 176
Query: 183 VRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEE 242
+ LR + V H H+D GLA+ N+L+AI+AG + NGIGERAG +
Sbjct: 177 LSLLRHEFPD---VVFEYHVHNDRGLALGNTLAAIQAGVQWHSASCNGIGERAGITDTFQ 233
Query: 243 VIVALDVRRDFYKVKTD-VNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQD 301
++ L K D NLK + S+LV + I+ P +VG NAF H + +HQ
Sbjct: 234 LLTLLHT-----KFAADRFNLKNVLAVSELVEAHSRIKRSPMHPVVGENAFVHVARLHQL 288
Query: 302 GVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRH-------AFRKKLEELGY 345
+ + + Y DP+ + ++ L +++ H F K EL Y
Sbjct: 289 AMQENQDAYSAFDPELI---NGRVSLQRYTPMHRQSLFLTPFEKSASELKY 336
>gb|AAF66618.1|AF142777_1 (AF142777) homocitrate synthase [Neurospora crassa]
Length = 270
Score = 142 bits (355), Expect = 7e-33
Identities = 87/255 (34%), Positives = 136/255 (53%), Gaps = 6/255 (2%)
Query: 125 VEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVPDTVGYATPEEFYELVR 184
+E+ +V FS+ED+ RSD L+ +Y AV G + + DTVG A+P + Y+LVR
Sbjct: 2 IEYVKSKGLEVRFSSEDSFRSDLVDLLSLYRAVDKVGVHRVGIADTVGCASPRQVYDLVR 61
Query: 185 YLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVI 244
LR +S I H HDD G A+AN+ A+ AGA ++ +V GIGER G L ++
Sbjct: 62 TLRGVVS----CDIETHFHDDTGCAIANAYCALEAGATHIDTSVLGIGERNGITPLGGLM 117
Query: 245 VALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVL 304
+ V Y VK+ L ++ LV+ I P N I G AF H++GIH +L
Sbjct: 118 ARMIVTSPDY-VKSKYKLHKLKELEDLVAEAVEINTPFNNPITGFCAFTHKAGIHAKAIL 176
Query: 305 KERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIAD 364
+TYEI++P G + + +G +A + ++ +LG +T++ ++ K K +AD
Sbjct: 177 NNPSTYEILNPADFGLTRYVHFASRLTGWNAVKTRVGQLGLEMTDDQVKECTAKIKALAD 236
Query: 365 RKRWITDTDIEAIIQ 379
R I D ++II+
Sbjct: 237 -VRPIAIDDADSIIR 250
>pir||C83170 2-isopropylmalate synthase PA3792 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9949965|gb|AAG07179.1|AE004798_2 (AE004798)
2-isopropylmalate synthase [Pseudomonas aeruginosa]
Length = 592
Score = 139 bits (348), Expect = 5e-32
Identities = 132/507 (26%), Positives = 227/507 (44%), Gaps = 42/507 (8%)
Query: 11 LRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE---VEDP 67
LRDG Q+ + +K+ K L ++ + IE FP AS+ +F V+++ +D
Sbjct: 76 LRDGNQSLIEPMDAAKKMRFFKTLVQVGLKQIEVAFPSASDTDFNFVRELIEGNHIPDDV 135
Query: 68 TIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAVEH 127
TI L +A E I R E+LR A+K +H + AT+P + ++ + V +A A +
Sbjct: 136 TIQVLTQAREDLITRTFESLRGAKKAIVHVYNATAPSFRRIVFNQDKQGVVDIATNAAKL 195
Query: 128 ATKYTED-------VEFSAEDATRSDWDFLVEVYEAVIDA------GATVINVPDTVGYA 174
K + ++S E + ++ +F VEV AVID ++N+P TV A
Sbjct: 196 IKKLAAEQPETQWSFQYSPEIFSSTELEFSVEVCNAVIDVWQPTPDNKIILNLPATVECA 255
Query: 175 TPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGER 234
TP + + + + R++ V IS+H H+D G VA + + AGAD+VE + G GER
Sbjct: 256 TPNVYADQIEWFGRHVDKRDSVIISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGER 315
Query: 235 AGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAH 294
GN L V L + + ++ +I K+V + V P VG
Sbjct: 316 TGNVDL----VTLALNMYTQGLHPQLDFSDIDAVRKVVEECNQLPVHPRHPYVGDLVHTA 371
Query: 295 ESGIHQDGV------LKERTTYEI----IDPKKLGFSGSKIV-LGKHSGRHAFRKKLE-E 342
SG HQD + KE +E+ IDP +G ++ + SG+ LE E
Sbjct: 372 FSGSHQDAIRKGFAQQKEDAIWEVPYLPIDPADIGRDYEAVIRVNSQSGKGGITFLLEQE 431
Query: 343 LGYSLTEEHLERAFKKFKDIADRKRW-ITDTDIEAIIQEELTKSNGK--LKVEIIHVTSG 399
G SL + + DR +T I ++++ E K+ L + +G
Sbjct: 432 YGISLPRRMQIEFSQVVQGETDRLGLEMTAQQIYSLLENEYLKATSPYVLASHRLQEENG 491
Query: 400 KVSTATVRISMNGEERIEVAWFKNGPIDALFSAINKALGEEFKLREYRVSSVTSGKDSLG 459
S + +S +GE++ GP++AL + AL + ++ +Y ++ +G ++
Sbjct: 492 -TSAVDLEVSFDGEKQ-HWRGIGKGPLEALVA----ALPVKAEIMDYHEHAIGAGANAKA 545
Query: 460 EVLVRVEVNGE-IFVGRGLSTDIIEAS 485
+ + + G+ G G+ +I AS
Sbjct: 546 AAYIEIRLEGQRPLHGIGIDENITTAS 572
>sp|P70728|NIFV_AZOBR HOMOCITRATE SYNTHASE >gi|1597740|gb|AAC46178.1| (U26427) nifV
[Azospirillum brasilense]
Length = 376
Score = 132 bits (330), Expect = 6e-30
Identities = 104/323 (32%), Positives = 151/323 (46%), Gaps = 29/323 (8%)
Query: 6 IFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATEVE 65
I DT RDGEQT GV+ T++EK+ IA+ L + V +E G P E + ++ +A+
Sbjct: 7 INDTHARDGEQTAGVAFTLDEKIAIAQALDEAGVAELEIGIPAMGREERERIRAVASLGL 66
Query: 66 DPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAVKAV 125
+ R + D+ A+ + + S IH+ KL + + V
Sbjct: 67 KARLMVWCRMHDTDLKARSTAVAPSLS------VPVSDIHITKKLNGSRAWALREIERKV 120
Query: 126 EHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVP-----DTVGYATPEEFY 180
+ A + +V ED++R+D DFL+ A ATV P DT+G P +
Sbjct: 121 KTARDHGLEVSLGGEDSSRADMDFLI--------AAATVAQRPRFRFADTLGVLDPFQHA 172
Query: 181 ELVRYLRRNISNIKGVQISVHCHDD-LGLAVA-NSLSAIRAGADQVEVTVNGIGERAGNA 238
L + +I H H L A A +L+A+ GA V TVNG+GERAGNA
Sbjct: 173 RL-----HLTAAATDPEIGYHAHTPRLANATAWVTLAAVLGGATHVNTTVNGLGERAGNA 227
Query: 239 ALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGI 298
LEEV+V+L V Y V+ + + S LV + V NK+IV F HE+GI
Sbjct: 228 PLEEVVVSLKV---LYGQDCGVDTRALGAISDLVERASNRPVAVNKSIVRDAVFTHEAGI 284
Query: 299 HQDGVLKERTTYEIIDPKKLGFS 321
H DG+L +R I P + G S
Sbjct: 285 HVDGLLTDRAPTRISTPPRWGAS 307
>emb|CAA91564.1| (Z67750) putative protein [Saccharomyces cerevisiae]
>gi|1903292|emb|CAA98756.1| (Z74229) ORF YDL182w
[Saccharomyces cerevisiae]
Length = 254
Score = 122 bits (303), Expect = 9e-27
Identities = 71/219 (32%), Positives = 117/219 (53%), Gaps = 6/219 (2%)
Query: 161 GATVINVPDTVGYATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAG 220
G + + DTVG A P + YEL+R L+ +S I H H+D G A+AN+ +A+ G
Sbjct: 2 GVNRVGIADTVGCANPRQVYELIRTLKSVVS----CDIECHFHNDTGCAIANAYTALEGG 57
Query: 221 ADQVEVTVNGIGERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEV 280
A ++V+V GIGER G L ++ + V Y VK+ L +I LV+ + +
Sbjct: 58 ARLIDVSVLGIGERNGITPLGGLMARMIVAAPDY-VKSKYKLHKIRDIENLVADAVEVNI 116
Query: 281 PPNKAIVGGNAFAHESGIHQDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGRHAFRKKL 340
P N I G AF H++GIH +L +TYEI+DP G + +G +A + ++
Sbjct: 117 PFNNPITGFCAFTHKAGIHAKAILANPSTYEILDPHDFGMKRYIHFANRLTGWNAIKARV 176
Query: 341 EELGYSLTEEHLERAFKKFKDIADRKRWITDTDIEAIIQ 379
++L +LT++ ++ K K + D R + D+++II+
Sbjct: 177 DQLNLNLTDDQIKEVTAKIKKLGD-VRSLNIDDVDSIIK 214
>sp|P96420|LEU1_MYCTU 2-ISOPROPYLMALATE SYNTHASE (ALPHA-ISOPROPYLMALATE SYNTHASE)
(ALPHA-IPM SYNTHETASE) >gi|7447670|pir||G70794 probable
alpha-isopropyl malate synthase - Mycobacterium
tuberculosis (strain H37RV) >gi|2960134|emb|CAA18032.1|
(AL022121) leuA [Mycobacterium tuberculosis]
Length = 644
Score = 120 bits (297), Expect = 5e-26
Identities = 125/503 (24%), Positives = 216/503 (42%), Gaps = 52/503 (10%)
Query: 11 LRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE---VEDP 67
LRDG Q ++ K + L ++ IE GFP AS+ +F V++I + +D
Sbjct: 79 LRDGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQTDFDFVREIIEQGAIPDDV 138
Query: 68 TIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVKKLAV----K 123
TI L + + I+R +A A + +H + +TS + + R EV+ +A K
Sbjct: 139 TIQVLTQCRPELIERTFQACSGAPRAIVHFYNSTSILQRRVVFRANRAEVQAIATDGARK 198
Query: 124 AVEHATKYTED---VEFSAEDATRSDWDFLVEVYEAVIDAGAT------VINVPDTVGYA 174
VE A KY E+S E T ++ ++ +V +AV + A + N+P TV
Sbjct: 199 CVEQAAKYPGTQWRFEYSPESYTGTELEYAKQVCDAVGEVIAPTPERPIIFNLPATVEMT 258
Query: 175 TPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGER 234
TP + + + ++ RN++N + V +S+H H+D G AVA + AGAD++E + G GER
Sbjct: 259 TPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELGFAAGADRIEGCLFGNGER 318
Query: 235 AGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAH 294
GN L V L + V ++ I + V + + V G +
Sbjct: 319 TGNVCL----VTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPVHERHPYGGDLVYTA 374
Query: 295 ESGIHQDGVLKE--------------------RTTYEIIDPKKLGFSGSKIV-LGKHSGR 333
SG HQD + K + Y IDP+ +G + ++ + SG+
Sbjct: 375 FSGSHQDAINKGLDAMKLDADAADCDVDDMLWQVPYLPIDPRDVGRTYEAVIRVNSQSGK 434
Query: 334 H--AFRKKLE---ELGYSLTEEHLERAFKKFKDIADRKRWITDTDIEAIIQEELTKSNGK 388
A+ K + L L E + K + A ++ ++ EE
Sbjct: 435 GGVAYIMKTDHGLSLPRRLQIEFSQVIQKIAEGTAGEGGEVSPKEMWDAFAEEYLAPVRP 494
Query: 389 LKVEIIHVTS----GKVSTATVRISMNGEERIEVAWFKNGPIDALFSAINKALGEEFKLR 444
L+ HV + G ++ T + +NG E E++ NGP+ A A+ +G + +
Sbjct: 495 LERIRQHVDAADDDGGTTSITATVKINGVE-TEISGSGNGPLAAFVHAL-ADVGFDVAVL 552
Query: 445 EYRVSSVTSGKDSLGEVLVRVEV 467
+Y ++++G D+ V V
Sbjct: 553 DYYEHAMSAGDDAQAAAYVEASV 575
>emb|CAB60077.1| (Y18874) 2-isopropylmalate synthase [Synechococcus PCC6301]
Length = 268
Score = 104 bits (258), Expect = 2e-21
Identities = 77/259 (29%), Positives = 128/259 (48%), Gaps = 12/259 (4%)
Query: 4 VYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIATE 63
+ ++DTTLRDG Q G+SL++E+K+ IA L +L V IE G+P A+ + + ++
Sbjct: 9 IAVYDTTLRDGAQREGLSLSLEDKLRIAHCLDRLGVKFIEGGWPGANPKDVQFFWELQQR 68
Query: 64 VEDPTIAALARAVEKDIDRAGE-----ALRNAEKNRIHTFIATSPIHMKYKLRKEPEEVK 118
+ + AGE AL A + F + +H+ L+ +E
Sbjct: 69 PLQQAEVVAFCSTRRPGQIAGEDELLKALLAAGTTWVTIFGKSWDLHVVEGLKTSLDENL 128
Query: 119 KLAVKAVEHATKYTEDVEFSAE---DATRSDWDFLVEVYEAVIDAGATVINVPDTVGYAT 175
+ ++ + V + AE D ++ D+ ++ A I+AGA I + DT G
Sbjct: 129 VMISDSIAYLRTCDRRVIYDAEHWFDGYLANPDYALQTLAAAIEAGAEWIVLCDTNGGCL 188
Query: 176 PEEFYELVRYLRRNISNIK----GVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGI 231
P + E+V + ++ G Q+ +H H+D AVAN+++A++AGA V T+NG
Sbjct: 189 PHQISEIVAAVLDRFPSLAPDQTGPQLGIHTHNDSETAVANAIAAVQAGARMVHGTINGY 248
Query: 232 GERAGNAALEEVIVALDVR 250
GER GNA L VI L ++
Sbjct: 249 GERCGNANLCSVIPNLQLK 267
>pir||F57256 2-isopropylmalate synthase homolog - Haemophilus influenzae
(fragment)
Length = 91
Score = 83.1 bits (202), Expect = 6e-15
Identities = 39/72 (54%), Positives = 57/72 (79%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
+V IFDTTLRDGEQ SLTV+EK++IA L +L VDV+E GFP++S+G+F++V+ IA
Sbjct: 20 RVIIFDTTLRDGEQALKASLTVKEKLQIALALERLGVDVMEVGFPVSSQGDFESVQTIAR 79
Query: 63 EVEDPTIAALAR 74
+++ +AAL+R
Sbjct: 80 HIKNARVAALSR 91
>gb|AAA70115.1| (U18657) LeuA [Haemophilus influenzae]
Length = 74
Score = 81.1 bits (197), Expect = 2e-14
Identities = 38/71 (53%), Positives = 56/71 (78%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIAT 62
+V IFDTTLRDGEQ SLTV+EK++IA L +L VDV+E GFP++S+G+F++V+ IA
Sbjct: 4 RVIIFDTTLRDGEQALKASLTVKEKLQIALALERLGVDVMEVGFPVSSQGDFESVQTIAR 63
Query: 63 EVEDPTIAALA 73
+++ +AAL+
Sbjct: 64 HIKNARVAALS 74
>emb|CAA19166.1| (AL023596) 2-isopropylmalate synthase [Mycobacterium leprae]
Length = 342
Score = 80.4 bits (195), Expect = 4e-14
Identities = 84/338 (24%), Positives = 139/338 (40%), Gaps = 41/338 (12%)
Query: 179 FYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTVNGIGERAGNA 238
+ + + ++ RN++N + V +S+H H+D G AVA + AGAD++E + G GER GN
Sbjct: 1 YADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELGYHAGADRIEGCLFGNGERTGNV 60
Query: 239 ALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPNKAIVGGNAFAHESGI 298
L V L + V ++ I + V + + V G + SG
Sbjct: 61 CL----VTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNKLRVHERHPYGGDLVYTAFSGS 116
Query: 299 HQDGVLKE--------------------RTTYEIIDPKKLGFSGSKIV-LGKHSGRH--A 335
HQD + K + Y IDP+ +G + ++ + SG+ A
Sbjct: 117 HQDAINKGLDQMKIDADAADSDVDDILWQVPYLPIDPRDVGRTYEAVIRVNSQSGKGGVA 176
Query: 336 FRKKLE---ELGYSLTEEHLERAFKKFK-----DIADRKRWITDTDIEAIIQEELTKSNG 387
+ K + EL L E RA +K +I + W D E +
Sbjct: 177 YIMKADHGLELPRRLQIE-FSRAIQKISEGEGGEITPTEMW--DVFFEEYLSPVQPLERI 233
Query: 388 KLKVEIIHVTSGKVSTATVRISMNGEERIEVAWFKNGPIDALFSAINKALGEEFKLREYR 447
K +V G S A + +NGEE E++ NGP+ A A+ +G + + +Y
Sbjct: 234 KQRVNAAEEDGGSTSIAAT-VKINGEE-TEISGVGNGPLAAFIDALGH-VGLQVAVLDYS 290
Query: 448 VSSVTSGKDSLGEVLVRVEVNGEIFVGRGLSTDIIEAS 485
++ +G D+ V V+G G G++ I AS
Sbjct: 291 EHAMNAGDDAQAAAYVEASVHGHTAWGVGIAPSITTAS 328
>dbj|BAA90528.1| (AB029822) 2-phosphinomethylmalic acid synthase [Streptomyces
hygroscopicus]
Length = 440
Score = 78.8 bits (191), Expect = 1e-13
Identities = 95/352 (26%), Positives = 144/352 (39%), Gaps = 31/352 (8%)
Query: 3 KVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAG----FPIASEGEFKAVK 58
+V++ +TT RDG+Q G+ L+++ I L ++ D FP +
Sbjct: 38 EVWLSETTHRDGQQG-GLPLSLDTSRRIYDILCEITGDSTAIRHAEFFPYRDSDRNALIY 96
Query: 59 KIATEVEDPTIAALA--RAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEPE- 115
+ + I RA +D+ E ++ ++S H +K
Sbjct: 97 ALERHRDGAPIEPTTWIRARREDV----ELIKRIGVIETGLLSSSSDYHTFHKFGSGGRT 152
Query: 116 EVKKLAVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATV-------INVP 168
+ + + AV A + ED TRS DF+ + E V+ V
Sbjct: 153 QAASMYLDAVTMALDHGIRPRVHLEDTTRSSPDFVRALVEEVLKTAERYPAELQPRFRVC 212
Query: 169 DTVGYATPEEFYELVRYLRRNISNIKGV-----QISVHCHDDLGLAVANSLSAIRAGADQ 223
DT+G P + L R + R I ++G QI +H H+D L VAN L+AIR G
Sbjct: 213 DTLGIGLPYDDVSLPRSIPRWIRLLRGFGLSPSQIELHPHNDTWLVVANCLAAIREGCGV 272
Query: 224 VEVTVNGIGERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLVSHLTGIEVPPN 283
+ T G GER GNA LE V+V L Y VNL + +KLV GI P+
Sbjct: 273 ISGTTLGTGERTGNAPLEAVMVHL--LGMGYWSGARVNLPAV---NKLVELYEGIGAGPS 327
Query: 284 K--AIVGGNAFAHESGIHQDGVLKERTTYEIIDPKKLGFSGSKIVLGKHSGR 333
+ G +A+ +GIH DG+ K Y + L + L K SG+
Sbjct: 328 QKYPFFGRDAYVTRAGIHADGLNKFWWMYAPFNAPLLTGRELDVALTKDSGQ 379
>pir||B70567 probable mhpE protein - Mycobacterium tuberculosis (strain H37RV)
>gi|2104392|emb|CAB08693.1| (Z95390) mhpE [Mycobacterium
tuberculosis]
Length = 336
Score = 74.9 bits (181), Expect = 2e-12
Identities = 71/283 (25%), Positives = 111/283 (39%), Gaps = 37/283 (13%)
Query: 2 RKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIA------------ 49
R+ + DTT+RDG T ++ I L + IE G +
Sbjct: 8 REPIVLDTTVRDGSYAVNFQYTDDDVRRIVGDLDAAGIPYIEIGHGVTIGAAAAQGPAAH 67
Query: 50 -SEGEFKAVKKIATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKY 108
E F+A + + + A A + +D AG+ L F+ I ++
Sbjct: 68 TDEEYFRAARSVVRNARLGAVIVPALARIETVDLAGDYL---------DFLRICVIATEF 118
Query: 109 KLRKEPEEVKKLAVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINVP 168
+L + VE A +V + + D L + D G ++ V
Sbjct: 119 EL----------VMPFVERAQSKGLEVSIQLVKSHLFEPDVLAAAGKRARDVGVRIVYVV 168
Query: 169 DTVGYATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVTV 228
DT G PE+ V LR V + H H++L +AVAN+L A AGAD ++ T+
Sbjct: 169 DTTGTFLPEDARRYVEALR----GASDVSVGFHGHNNLAMAVANTLEAFDAGADFLDGTL 224
Query: 229 NGIGERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKL 271
G G AGN +E ++ AL R V D + + AR+ L
Sbjct: 225 MGFGRGAGNCQIECLVAALQRRGHLAAVDLD-RIFDAARSDML 266
>gb|AAD02153.1| (AF039534) 2-oxo-4-hydroxypentanoate aldolase [Pseudomonas
stutzeri]
Length = 346
Score = 73.4 bits (177), Expect = 5e-12
Identities = 73/281 (25%), Positives = 124/281 (43%), Gaps = 44/281 (15%)
Query: 2 RKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEA-------------GFPI 48
+ V + D +LRDG +++E+ + +A L + +IE GFP
Sbjct: 6 KNVTLHDMSLRDGMHAKRHQISLEQMIAVATGLDAAGMPLIEITHGDGLGGRSINYGFPA 65
Query: 49 ASEGEFKAVKKIATEVEDPTIAALARAVEKDIDRAGEALR-NAEKNRIHTFIATSPI--- 104
S+ E+ ++ + ++ ++AL +D AL R+ T + +
Sbjct: 66 HSDEEY--LRAVIPRLKQAKVSALLLPGIGTVDHLKMALDCGVSTIRVATHCTEADVSEQ 123
Query: 105 HMKYKLRKEPEEVKKLAVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATV 164
H+ + + V L + + A K E L+E Y GA
Sbjct: 124 HIGMSRKLGADTVGFLMMAHMISAEKVLEQAR-------------LMESY------GANC 164
Query: 165 INVPDTVGYATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQV 224
I D+ GY P+E E + LR ++ +I H H ++G+A+ANSL+AI AGA ++
Sbjct: 165 IYCTDSAGYMLPDEVSEKIGLLRAELN--PATEIGFHGHHNMGMAIANSLAAIEAGASRI 222
Query: 225 EVTVNGIGERAGNAALEEVIVALDVRRDFYKVKTDVNLKEI 265
+ +V G+G AGN L EV VA+ R V+T ++L +I
Sbjct: 223 DGSVAGLGAGAGNTPL-EVFVAVCKRMG---VETGIDLYKI 259
>sp|Q00852|NIVO_CLOPA HOMOCITRATE SYNTHASE, OMEGA SUBUNIT >gi|98608|pir||A39403 probable
homocitrate synthase (EC 4.1.3.21) NifV alpha chain -
Clostridium pasteurianum >gi|144878|gb|AAA23267.1|
(M68878) nitrogen fixation protein [Clostridium
pasteurianum]
Length = 352
Score = 72.6 bits (175), Expect = 9e-12
Identities = 52/174 (29%), Positives = 91/174 (51%), Gaps = 9/174 (5%)
Query: 208 LAVANSLSAIRAGADQVEVTVNGIGERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIAR 267
+A A ++A G+D + N AA+EEVI+AL R+ +++ D LK I++
Sbjct: 180 MATAIIINAFLNGSDIITTEFNS----NDYAAMEEVIIALKSIRNI-EIRGD--LKLISK 232
Query: 268 TSKLVSHLTGIEVPPNKAIVGGNAFAHESGIHQDGVLKERTTYEIIDPKKLGFSGSKIVL 327
+++ +T V K I+G + F +ESGIH DG+ K YE +P+ +G + K+ +
Sbjct: 233 LTRIYEKITSERVYSMKPILGEDIFKYESGIHADGIAKNPKNYEPFNPELIG-TNRKLYI 291
Query: 328 GKHSGRHAFRKKLEELGYSLTEEHLERAFKKFKDIA-DRKRWITDTDIEAIIQE 380
GKHSG+ A K +EL + + + ++ + KR + D +I + +E
Sbjct: 292 GKHSGKAALVVKFKELNLNCNNIDMNLFLQDIREKSIQEKRNVLDNEIIEMYKE 345
>sp|P51016|DMPG_PSESP 4-HYDROXY-2-OXOVALERATE ALDOLASE (HOA) >gi|94900|pir||S24420 dmpG
protein - Pseudomonas putida >gi|45684|emb|CAA43227.1|
(X60835) 4-hydroxy-2-oxovalerate aldolase [Pseudomonas
putida]
Length = 345
Score = 71.8 bits (173), Expect = 2e-11
Identities = 69/262 (26%), Positives = 111/262 (42%), Gaps = 36/262 (13%)
Query: 2 RKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEG--------- 52
+K+YI D TLRDG T+++ IA+ L K VD IE +G
Sbjct: 7 KKLYISDVTLRDGSHAIRHQYTLDDVRAIARALDKAKVDSIEVAHGDGLQGSSFNYGFGR 66
Query: 53 --EFKAVKKIATEVEDPTIAALAR---AVEKDIDRAGEALRNAEKNRIH-TFIATSPIHM 106
+ + ++ +A E+ IA L D+ A +A + H T S H+
Sbjct: 67 HTDLEYIEAVAGEISHAQIATLLLPGIGSVHDLKNAYQAGARVVRVATHCTEADVSKQHI 126
Query: 107 KYKLRKEPEEVKKLAVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVIN 166
+Y + V L + + A K E + L+E Y GAT I
Sbjct: 127 EYARNLGMDTVGFLMMSHMIPAEKLAEQGK-------------LMESY------GATCIY 167
Query: 167 VPDTVGYATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEV 226
+ D+ G + + + +R + + Q+ +H H +L L VANS+ A+ G D+V+
Sbjct: 168 MADSGGAMSMNDIRDRMRAFKAVLK--PETQVGMHAHHNLSLGVANSIVAVEEGCDRVDA 225
Query: 227 TVNGIGERAGNAALEEVIVALD 248
++ G+G AGNA LE I +
Sbjct: 226 SLAGMGAGAGNAPLEVFIAVAE 247
>dbj|BAB04853.1| (AP001511) hydroxymethylglutaryl-CoA lyase [Bacillus halodurans]
Length = 303
Score = 71.0 bits (171), Expect = 3e-11
Identities = 71/291 (24%), Positives = 128/291 (43%), Gaps = 35/291 (12%)
Query: 2 RKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAG-------FPIASEGEF 54
+KV I + RDG Q + E+K+E L++ + IE P ++ E
Sbjct: 5 KKVSIREVGPRDGLQNESTWIDTEQKIEWINHLSRTGLPYIEVSSFVHPKWIPALADAE- 63
Query: 55 KAVKKIATEVEDPTIAALARAVEKDIDRAGEALRNAEKNRIHTFIATSPIHMKYKLRKEP 114
V V+ T AAL EK ++RA A + + F++ + H + + K
Sbjct: 64 -EVLARIDRVDGVTYAALVPN-EKGLERA----LRAGVDEVAIFMSATETHNRKNINKSI 117
Query: 115 EE-------VKKLAVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVIDAGATVINV 167
+ V ++A + + Y V F ++ L++ EA++ G +++
Sbjct: 118 ADTLPVLKNVTRIAKQEGKSVRGYLSTV-FGCPYEGTTEQTKLLQTVEALMTMGIDELSL 176
Query: 168 PDTVGYATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIRAGADQVEVT 227
DT+G ATP +VR + + + K +++H HD G+A+AN +R G + + +
Sbjct: 177 GDTIGVATPNLVEAVVREVTKLVPTEK---MALHLHDTKGMAIANIACGLRTGVRKFDSS 233
Query: 228 VNGIG------ERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLV 272
V G+G AGN A E+++ L +TDV+L EI + + +
Sbjct: 234 VGGLGGCPYAPGAAGNVATEDLLYLL----HGLGYETDVSLNEIMKAAAYI 280
>pir||D69893 hydroxymethylglutaryl-CoA lyase homolog yngG - Bacillus subtilis
>gi|2266426|emb|CAA74217.1| (Y13917) yngG [Bacillus
subtilis] >gi|2634206|emb|CAB13706.1| (Z99113) similar
to hydroxymethylglutaryl-CoA lyase [Bacillus subtilis]
Length = 299
Score = 69.9 bits (168), Expect = 6e-11
Identities = 75/309 (24%), Positives = 131/309 (42%), Gaps = 53/309 (17%)
Query: 2 RKVYIFDTTLRDGEQTPGVSLTVEEKVEIAKQLAKLNVDVIEAGFPIASEGEFKAVKKIA 61
+KV I + RDG Q V + E+K+ QL++ + IE I
Sbjct: 5 KKVTIKEVGPRDGLQNEPVWIATEDKITWINQLSRTGLSYIE----------------IT 48
Query: 62 TEVEDPTIAALARAVE--KDIDRAG-----------EALRNAEKNRIH---TFIATSPIH 105
+ V I AL A++ K IDR L NA + I+ F++ S H
Sbjct: 49 SFVHPKWIPALRDAIDVAKGIDREKGVTYAALVPNQRGLENALEGGINEACVFMSASETH 108
Query: 106 MKYKLRKEPEE-------VKKLAVKAVEHATKYTEDVEFSAEDATRSDWDFLVEVYEAVI 158
+ + K E V A KA Y V F + ++ + EA+
Sbjct: 109 NRKNINKSTSESLHILKQVNNDAQKANLTTRAYLSTV-FGCPYEKDVPIEQVIRLSEALF 167
Query: 159 DAGATVINVPDTVGYATPEEFYELVRYLRRNISNIKGVQISVHCHDDLGLAVANSLSAIR 218
+ G + +++ DT+G A P + ++ L ++ QI++H HD G A+AN ++A++
Sbjct: 168 EFGISELSLGDTIGAANPAQVETVLEAL---LARFPANQIALHFHDTRGTALANMVTALQ 224
Query: 219 AGADQVEVTVNGIG------ERAGNAALEEVIVALDVRRDFYKVKTDVNLKEIARTSKLV 272
G + + G+G +GNAA E+++ L+ +KT+V L+++ +K +
Sbjct: 225 MGITVFDGSAGGLGGCPYAPGSSGNAATEDIVYMLEQ----MDIKTNVKLEKLLSAAKWI 280
Query: 273 SHLTGIEVP 281
G +P
Sbjct: 281 EEKMGKPLP 289
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.315 0.134 0.364
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 165851641
Number of Sequences: 2977
Number of extensions: 6818568
Number of successful extensions: 17331
Number of sequences better than 1.0e-10: 118
Number of HSP's better than 0.0 without gapping: 68
Number of HSP's successfully gapped in prelim test: 50
Number of HSP's that attempted gapping in prelim test: 16947
Number of HSP's gapped (non-prelim): 123
length of query: 486
length of database: 189,106,746
effective HSP length: 62
effective length of query: 424
effective length of database: 152,000,552
effective search space: 64448234048
effective search space used: 64448234048
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 42 (22.0 bits)
S2: 167 (69.5 bits)