BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0891 (leuC-1) DE:PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE
LARGE SUBUNIT (EC 4.2.1.33) (ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM
ISOMERASE) (IPMI).
(423 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||B75045 probable 3-isopropylmalate dehydratase (EC 4.2.1.33)... 856 0.0
sp|O67078|LEU2_AQUAE 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 585 e-166
pir||H72362 3-isopropylmalate dehydratase, large subunit - Therm... 490 e-137
sp|O27439|LE21_METTH PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE LARG... 443 e-123
sp|P81291|LE22_METJA PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE LARG... 430 e-119
sp|O27668|LE22_METTH PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE LARG... 429 e-119
gi|11499545 aconitase (acn) [Archaeoglobus fulgidus] >gi|3122334... 418 e-116
pir||C72394 hypothetical protein TM0291 - Thermotoga maritima (s... 400 e-110
pir||H75354 3-isopropylmalate dehydratase, large subunit - Deino... 399 e-110
gi|11499781 3-isopropylmalate dehydratase, large subunit (leuC) ... 381 e-105
sp|Q58409|LE21_METJA PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE LARG... 365 e-100
dbj|BAA74762.1| (AB017109) HacA [Thermus thermophilus] 337 1e-91
pir||T06300 hypothetical protein T9E8.170 - Arabidopsis thaliana... 323 2e-87
pir||D75373 3-isopropylmalate dehydratase, large subunit - Deino... 314 9e-85
pir||A71181 probable isomerase - Pyrococcus horikoshii >gi|32581... 296 2e-79
pir||H75159 3-isopropylmalate dehydratase, large chain (leuc-2) ... 295 5e-79
gb|AAK42608.1| 3-isopropylmalate dehydratase, large subunit (iso... 291 8e-78
pir||T29083 3-isopropylmalate dehydratase (EC 4.2.1.33) large ch... 262 5e-69
pir||T51171 homoaconitate hydratase (EC 4.2.1.36) [validated] - ... 257 2e-67
gb|AAG20620.1| (AE005132) aconitase; Can [Halobacterium sp. NRC-1] 254 1e-66
pir||T38665 probable homoaconitate hydratase (EC 4.2.1.36) - fis... 252 5e-66
emb|CAC14578.1| (AJ296268) 2-isopropylmalate isomerase large sub... 249 5e-65
dbj|BAA37138.1| (AB017135) isopropylmalate isomerase large subun... 247 1e-64
pir||F70453 aconitase - Aquifex aeolicus >gi|2984078|gb|AAC07617... 246 4e-64
gi|10957109 3-isopropylmalate dehydratase [Buchnera sp. APS] >gi... 246 4e-64
sp|P55251|LEU2_RHIPU 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMAL... 245 7e-64
sp|O33123|LEU2_MYCLE 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 245 9e-64
sp|P56934|LEU2_BUCAI 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 244 2e-63
gi|6320440 homoaconitase; Lys4p [Saccharomyces cerevisiae] >gi|1... 244 2e-63
emb|CAA72703.1| (Y11966) 3-isopropylmalate dehydratase subunit [... 242 8e-63
sp|Q44427|LEU2_ACTTI 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 242 8e-63
sp|O53237|LEU2_MYCTU 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 241 1e-62
sp|P49601|LEU2_USTMA 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMAL... 241 1e-62
dbj|BAB06775.1| (AP001517) 3-isopropylmalate dehydratase large s... 241 1e-62
sp|P80858|LEU2_BACSU 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 240 2e-62
sp|O14289|LEU2_SCHPO 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMAL... 236 3e-61
pir||S26864 3-isopropylmalate dehydratase (EC 4.2.1.33) - Phycom... 236 3e-61
sp|P18250|LEU2_PHYBL 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMAL... 236 3e-61
emb|CAB99455.1| (AJ278693) 3-isopropylmalate dehydratase [Yarrow... 236 3e-61
gi|10954460 3-isopropylmalate isomerase subunit [Buchnera aphidi... 236 5e-61
gi|6321429 isopropylmalate isomerase; Leu1p [Saccharomyces cerev... 235 1e-60
sp|Q00464|LEU2_CANMA 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMAL... 234 2e-60
sp|P17279|LEU2_RHIRA 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMAL... 233 2e-60
sp|P55811|LEU2_RHINI 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMAL... 232 5e-60
pir||H82070 3-isopropylmalate dehydratase, large chain VC2492 [s... 232 5e-60
gi|10954452 isopropylmalate isomerase subunit [Buchnera aphidico... 231 1e-59
gb|AAG31406.1| (AF197457) isopropylmaltate dehydratase subunit [... 231 2e-59
sp|P30127|LEU2_ECOLI 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 230 3e-59
gb|AAB19612.1| (S57886) isopropylmalate isomerase {EC 4.2.1.33} ... 229 3e-59
gb|AAG31388.1| (AF197451) isopropylmaltate dehydratase subunit [... 229 3e-59
sp|P44968|LEU2_HAEIN 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 229 4e-59
sp|Q92412|LYS4_EMENI HOMOACONITASE PRECURSOR (HOMOACONITATE HYDR... 229 4e-59
pir||H64728 3-isopropylmalate dehydratase (EC 4.2.1.33) alpha ch... 229 4e-59
sp|Q02142|LEU2_LACLA 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 228 1e-58
pir||S40586 3-isopropylmalate dehydratase (EC 4.2.1.33) alpha ch... 227 2e-58
gb|AAG31397.1| (AF197454) isopropylmaltate dehydratase subunit [... 227 2e-58
pir||G81128 3-isopropylmalate dehydratase, large chain NMB1036 [... 227 2e-58
pir||A81270 3-isopropylmalate dehydratase (EC 4.2.1.33) large ch... 226 3e-58
pir||A81836 probable 3-isopropylmalate dehydratase (EC 4.2.1.33)... 226 4e-58
gb|AAG31400.1| (AF197455) isopropylmaltate dehydratase subunit [... 226 5e-58
pir||G83255 3-isopropylmalate dehydratase large subunit PA3121 [... 224 1e-57
sp|P48573|LEU2_BUCRP 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 224 1e-57
gb|AAG31403.1| (AF197456) isopropylmaltate dehydratase subunit [... 224 2e-57
gb|AAG31394.1| (AF197453) isopropylmaltate dehydratase subunit [... 224 2e-57
gb|AAG31382.1| (AF197449) isopropylmaltate dehydratase subunit [... 223 3e-57
pir||D36889 3-isopropylmalate dehydratase (EC 4.2.1.33) chain le... 223 3e-57
gb|AAG31385.1| (AF197450) isopropylmaltate dehydratase subunit [... 222 4e-57
pir||H82564 3-isopropylmalate dehydratase large subunit XF2375 [... 217 1e-55
gb|AAG31379.1| (AF197448) isopropylmaltate dehydratase subunit [... 217 2e-55
gb|AAG31391.1| (AF197452) isopropylmaltate dehydratase subunit [... 217 3e-55
gb|AAG31929.1|AF200469_3 (AF200469) isopropylmalate dehydratase ... 210 3e-53
sp|P15717|LEU2_SALTY 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 210 3e-53
sp|P54384|LEU2_SYNY3 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 207 2e-52
pir||S10171 3-isopropylmalate dehydratase (EC 4.2.1.33) chain le... 187 2e-46
gb|AAA34389.1| (M33131) aconitase (ACO1) (EC 4.2.1.3) [Saccharom... 164 2e-39
gi|6323335 Aconitase, mitochondrial; Aco1p [Saccharomyces cerevi... 164 2e-39
gb|AAB41277.1| (U32936) 3-isopropylmalate dehydratase [Synechocy... 157 2e-37
gb|AAF54588.1| (AE003690) CG4706 gene product [Drosophila melano... 155 7e-37
sp|P49609|ACON_GRAVE ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSO... 150 3e-35
emb|CAB83173.1| (AL162692) aconitate hydratase [Schizosaccharomy... 149 5e-35
sp|O13966|ACON_SCHPO ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSO... 148 8e-35
gb|AAC61778.1| (AF093142) aconitase [Aspergillus terreus] 148 1e-34
emb|CAB72317.1| (AJ245736) aconitase [Daphnia pulex] 147 2e-34
gi|6322261 Yjl200cp [Saccharomyces cerevisiae] >gi|728783|sp|P39... 147 2e-34
emb|CAB93521.1| (AJ252018) mitochondrial aconitase [Drosophila m... 145 1e-33
emb|CAA88513.1| (Z48612) unknown [Saccharomyces cerevisiae] 140 2e-32
sp|P34455|ACON_CAEEL PROBABLE ACONITATE HYDRATASE, MITOCHONDRIAL... 138 9e-32
emb|CAA76360.1| (Y16747) aconitate hydratase [Piromyces sp. E2] 138 9e-32
gb|AAF53973.1| (AE003669) Acon gene product [alt 1] [Drosophila ... 137 2e-31
gi|4501867 aconitase 2; Aconitase, mitochondrial [Homo sapiens] ... 135 1e-30
emb|CAB46022.1| (AL023553) dJ347H13.1 (aconitase 2, mitochondria... 135 1e-30
sp|P16276|ACON_PIG ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSOR ... 134 1e-30
pdb|5ACN| Aconitase (E.C.4.2.1.3) (Inactive (3fe-4s) Cluster F... 134 1e-30
gb|AAB38416.1| (U80040) mitochondrial aconitase [Homo sapiens] 134 1e-30
pdb|1C96|A Chain A, S642a:citrate Complex Of Aconitase 134 1e-30
pdb|1C97|A Chain A, S642a:isocitrate Complex Of Aconitase >gi|67... 134 1e-30
pdb|1B0J|A Chain A, Crystal Structure Of Aconitase With Isocitrate 134 1e-30
pdb|1B0M|A Chain A, Aconitase R644q:fluorocitrate Complex 134 1e-30
sp|P20004|ACON_BOVIN ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSO... 134 2e-30
emb|CAC18623.1| (AL451109) related to aconitate hydratase precur... 134 2e-30
pdb|8ACN| Aconitase (E.C.4.2.1.3) Complex With Nitroisocitrate... 134 2e-30
pdb|1NIS| Aconitase (E.C.4.2.1.3) Complex With Nitrocitrate (M... 134 2e-30
pdb|1AMJ| Aconitase (E.C.4.2.1.3) Complexed With Sulfate And H... 134 2e-30
pir||S57528 aconitate hydratase (EC 4.2.1.3) precursor, mitochon... 134 2e-30
gb|AAD19351.2| (AF093096) aconitase [Homo sapiens] 134 2e-30
sp|Q44023|LEU2_ALCEU 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT... 133 4e-30
emb|CAC11018.1| (AJ243266) mitochondrial aconitase [Rattus norve... 132 7e-30
gb|AAF01428.1|AF186471_1 (AF186471) aconitase [Xenopus laevis] 129 5e-29
pir||S45196 3-isopropylmalate dehydrogenase homolog - Escherichi... 126 5e-28
sp|P36683|ACO2_ECOLI ACONITATE HYDRATASE 2 (CITRATE HYDRO-LYASE ... 126 5e-28
pir||C81356 aconitate hydratase (EC 4.2.1.3) Cj0835c [imported] ... 123 3e-27
pir||G83422 aconitate hydratase 2 PA1787 [imported] - Pseudomona... 122 8e-27
pir||C82303 aconitate hydratase 2 VC0604 [imported] - Vibrio cho... 119 5e-26
gb|AAC35557.1| (AF086790) aconitase precursor [Homo sapiens] 119 7e-26
sp|Q9ZL64|ACO2_HELPJ ACONITATE HYDRATASE 2 (CITRATE HYDRO-LYASE ... 116 5e-25
pir||C64617 aconitate hydratase (EC 4.2.1.3) 2 - Helicobacter py... 116 6e-25
sp|P56418|ACO2_HELPY ACONITATE HYDRATASE 2 (CITRATE HYDRO-LYASE ... 116 6e-25
pir||G81068 aconitate hydratase 2 NMB1572 [imported] - Neisseria... 113 3e-24
pir||A81801 aconitate hydratase (EC 4.2.1.3) NMA1761 [imported] ... 113 3e-24
pir||A82825 aconitate hydratase 2 XF0292 [imported] - Xylella fa... 113 3e-24
gb|AAD32176.1|AF125563_1 (AF125563) putative aconitate hydratase... 113 5e-24
emb|CAA89720.1| (Z49701) unknown [Saccharomyces cerevisiae] 112 9e-24
sp|P74582|ACO2_SYNY3 ACONITATE HYDRATASE 2 (CITRATE HYDRO-LYASE ... 111 1e-23
gb|AAK41354.1| Aconitate hydratase [Sulfolobus solfataricus] 108 1e-22
gb|AAF09127.1|AF088222_2 (AF088222) aconitate hydratase [Lactoco... 103 3e-21
sp|P37032|ACON_LEGPN ACONITATE HYDRATASE (CITRATE HYDRO-LYASE) (... 99 9e-20
sp|P25516|ACO1_ECOLI ACONITATE HYDRATASE 1 (CITRATE HYDRO-LYASE ... 96 6e-19
pir||G64875 aconitate hydratase (EC 4.2.1.3) - Escherichia coli ... 95 1e-18
pir||A83547 probable aconitate hydratase PA0794 [imported] - Pse... 95 2e-18
sp|Q9ZCF4|ACON_RICPR ACONITATE HYDRATASE (CITRATE HYDRO-LYASE) (... 90 6e-17
dbj|BAA21004.1| (D17631) ORF [Escherichia coli] >gi|4433055|dbj|... 85 1e-15
pir||C81200 aconitate hydratase 1 NMB0433 [imported] - Neisseria... 83 4e-15
pir||H81775 aconitate hydratase (EC 4.2.1.3) NMA2052 [imported] ... 78 2e-13
>pir||B75045 probable 3-isopropylmalate dehydratase (EC 4.2.1.33) large chain
PAB0891 - Pyrococcus abyssi (strain Orsay)
>gi|5458768|emb|CAB50255.1| (AJ248287) PUTATIVE
3-ISOPROPYLMALATE DEHYDRATASE LARGE GE SUBUNIT (EC
4.2.1.33) (ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM
ISOMERASE) (IPMI). [Pyrococcus abyssi]
Length = 423
Score = 856 bits (2187), Expect = 0.0
Identities = 423/423 (100%), Positives = 423/423 (100%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI
Sbjct: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
Query: 61 AIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLII 120
AIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLII
Sbjct: 61 AIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLII 120
Query: 121 GADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLIL 180
GADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLIL
Sbjct: 121 GADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLIL 180
Query: 181 HTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKE 240
HTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKE
Sbjct: 181 HTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKE 240
Query: 241 RAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQVFIG 300
RAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQVFIG
Sbjct: 241 RAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQVFIG 300
Query: 301 SCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPP 360
SCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPP
Sbjct: 301 SCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPP 360
Query: 361 TCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEE 420
TCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEE
Sbjct: 361 TCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEE 420
Query: 421 VVK 423
VVK
Sbjct: 421 VVK 423
>sp|O67078|LEU2_AQUAE 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7450352|pir||C70381 large subunit of isopropylmalate
isomerase - Aquifex aeolicus >gi|2983447|gb|AAC07028.1|
(AE000714) large subunit of isopropylmalate isomerase
[Aquifex aeolicus]
Length = 432
Score = 585 bits (1491), Expect = e-166
Identities = 284/428 (66%), Positives = 346/428 (80%), Gaps = 8/428 (1%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
MGMTI EKILA+H+ ++EV PGE++ AK+D NDVT PLAIK + G+ +VFD ERI
Sbjct: 1 MGMTITEKILAEHAGKKEVHPGELITAKIDLAMANDVTAPLAIKILEKYGIDKVFDPERI 60
Query: 61 AIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLII 120
A+VL HF P KDIKSAEQ K REF K+ GIKWFFE G G+EH +LPE GLV+PGDL++
Sbjct: 61 ALVLSHFVPAKDIKSAEQAKIVREFVKKHGIKWFFEEGE-GIEHAILPEQGLVVPGDLVV 119
Query: 121 GADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLIL 180
GADSHTCTYGALGAFATGVGSTD+A AMATGE W RVPE+MKFI+ G+L+P+V GKDLIL
Sbjct: 120 GADSHTCTYGALGAFATGVGSTDIAYAMATGEVWLRVPESMKFIFYGKLKPWVMGKDLIL 179
Query: 181 HTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKE 240
+TIG IGV+GALY+ MEF G I LS+EQR T++NMAIEAG K+GII PD+KT++YVK+
Sbjct: 180 YTIGQIGVDGALYRAMEFDGETIRNLSMEQRFTIANMAIEAGGKSGIISPDEKTIEYVKQ 239
Query: 241 RAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQVFIG 300
RAKR +KVY+SD DA+Y+ V E+D + EP+VA+P+LP N P+S++ +I IDQ FIG
Sbjct: 240 RAKRPWKVYQSDPDAEYHSVYEWDASQIEPLVAWPYLPSNVHPVSEST--HITIDQAFIG 297
Query: 301 SCTNGRIEDLRMAAEILE-----GQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGA 355
SCTNGRIEDLR+AA++ E G+KV +VR IVIP S +Y +ALKEGLI+IF+EAG
Sbjct: 298 SCTNGRIEDLRVAAKVFEAALKQGKKVHPYVRCIVIPASKAIYKQALKEGLIDIFMEAGC 357
Query: 356 VIGPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRI 415
V+ TCGPCLGGHMGVLA GER +ST+NRNF GRMGHPKSE YLANP V AASA++GRI
Sbjct: 358 VVSTSTCGPCLGGHMGVLAEGERCISTSNRNFPGRMGHPKSESYLANPAVVAASAIMGRI 417
Query: 416 ASPEEVVK 423
A P+EVVK
Sbjct: 418 AHPDEVVK 425
>pir||H72362 3-isopropylmalate dehydratase, large subunit - Thermotoga maritima
(strain MSB8) >gi|4981069|gb|AAD35639.1|AE001730_7
(AE001730) 3-isopropylmalate dehydratase, large subunit
[Thermotoga maritima]
Length = 417
Score = 490 bits (1248), Expect = e-137
Identities = 246/419 (58%), Positives = 314/419 (74%), Gaps = 2/419 (0%)
Query: 3 MTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIAI 62
MT+AEKIL+ + R+ V+PGE ++ + D ND+T PLAIKKF+E G K+V +R+ +
Sbjct: 1 MTLAEKILSQKAGRK-VEPGEFLLLEPDIALANDITAPLAIKKFKEYGGKKVKYPDRVVL 59
Query: 63 VLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLIIGA 122
V DHFTPNKDIKSA Q K REFA+E GI+ FFE G +G+EH LLPE G+V GDL++GA
Sbjct: 60 VPDHFTPNKDIKSAMQVKMMREFAREQGIEKFFEIGRMGIEHVLLPEEGIVKSGDLVVGA 119
Query: 123 DSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLILHT 182
DSHTCTYGALGAFATGVGSTD+A G+ WFRVPE++K G+ + VT KDL+L
Sbjct: 120 DSHTCTYGALGAFATGVGSTDIAGFYLIGKVWFRVPESIKVTLRGKFKDLVTAKDLVLKL 179
Query: 183 IGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKERA 242
I +GV+GA YK +EFSG ++E+S++ R T+SNMAIEAG KTG+ D+ T+ Y +ER
Sbjct: 180 ISILGVDGANYKAIEFSGPGVKEISMDGRFTISNMAIEAGGKTGLFPVDEITIAYERERG 239
Query: 243 KRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQVFIGSC 302
++Y DEDAKY + +E D++ EP VA+P LP N +S+A K IKIDQ IGSC
Sbjct: 240 IEVEEMY-PDEDAKYVREVEMDLSELEPQVAYPFLPSNAKDVSEAEKERIKIDQAVIGSC 298
Query: 303 TNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTC 362
TNGRIEDLR+AA+IL+G+ V+ VR I+IP S VY +ALKEGLI+IF++AG + PTC
Sbjct: 299 TNGRIEDLRLAAQILKGRTVSPDVRCIIIPGSQKVYKQALKEGLIDIFIDAGCAVSTPTC 358
Query: 363 GPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEEV 421
GPCLGGHMGVLA GE A+STTNRNFVGRMGHP S+V+LA+P VAAASA+ G IA P ++
Sbjct: 359 GPCLGGHMGVLAEGEVAISTTNRNFVGRMGHPNSKVFLASPAVAAASAIKGYIADPRKL 417
>sp|O27439|LE21_METTH PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7450350|pir||D69051 3-isopropylmalate dehydratase,
LeuD subunit - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2622496|gb|AAB85863.1| (AE000901)
3-isopropylmalate dehydratase, LeuD subunit
[Methanobacterium thermoautotrophicum]
Length = 419
Score = 443 bits (1126), Expect = e-123
Identities = 217/418 (51%), Positives = 305/418 (72%), Gaps = 3/418 (0%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M MT++EKILA S ++ V+ GEIVMA +D +D+T PL+++ FR +G RV+D E+I
Sbjct: 1 MSMTVSEKILARASGKDRVEAGEIVMADIDVAMTHDLTGPLSVESFRAIGEDRVWDPEKI 60
Query: 61 AIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLII 120
++ DH P I++A+ R+F +E GI+ F++ GV H +LPE G V+PG++++
Sbjct: 61 VVIFDHQVPADSIEAAQNHMIMRDFVEEQGIRNFYDVRE-GVCHQVLPEKGHVVPGEVVV 119
Query: 121 GADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLIL 180
G DSHTCT+GALGAFATG+GSTD+A+ ATG+ WFRVPET++F G+L+ +V KD+IL
Sbjct: 120 GTDSHTCTHGALGAFATGIGSTDMAMVFATGKLWFRVPETLRFDVRGKLREHVYAKDVIL 179
Query: 181 HTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKE 240
+ IG +G +GA Y EF+G + E+SV RM +SNMAIE G KTGI+EPD+KTL+YV+
Sbjct: 180 NIIGRVGADGATYMACEFAGETVAEMSVSDRMVLSNMAIEMGGKTGIVEPDEKTLNYVRR 239
Query: 241 RAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQVFIG 300
R+ + ++V+K+D DA V+E DV++ EP VA PH +N P+++ +IDQVF+G
Sbjct: 240 RSGKPWRVFKTDPDAPSLSVMEVDVSDLEPQVACPHNVDNVKPVTEVE--GTEIDQVFLG 297
Query: 301 SCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPP 360
SCTNGR+ DLR AA IL+ +KV+ VR++VIP S VY +AL EGLIEIF++AGA++ P
Sbjct: 298 SCTNGRLSDLRDAAAILKNRKVSDSVRMLVIPASREVYRRALDEGLIEIFVDAGALVCNP 357
Query: 361 TCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASP 418
CGPCLGGH+G++ GE ++ST+NRNF GR G P++EVYL++ VAAASAV G I P
Sbjct: 358 CCGPCLGGHVGLVGPGEVSLSTSNRNFRGRQGSPEAEVYLSSAAVAAASAVKGSITHP 415
>sp|P81291|LE22_METJA PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|2127740|pir||C64362 aconitate hydratase (EC 4.2.1.3)
- Methanococcus jannaschii >gi|1591201|gb|AAB98487.1|
(U67499) 3-isopropylmalate dehydratase (leuC)
[Methanococcus jannaschii]
Length = 424
Score = 430 bits (1094), Expect = e-119
Identities = 222/427 (51%), Positives = 293/427 (67%), Gaps = 9/427 (2%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
MGMTI EKILA S ++EV PG+IVMA +D +D+T PL + +E G+++V++ E+I
Sbjct: 1 MGMTIVEKILAKASGKKEVSPGDIVMANIDVAMVHDITGPLTVNTLKEYGIEKVWNPEKI 60
Query: 61 AIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLII 120
I+ DH P IK+AE R+F KE GIK+F++ GV H +LPE G V PG++++
Sbjct: 61 VILFDHQVPADSIKAAENHILMRKFVKEQGIKYFYDIRE-GVCHQVLPEKGHVAPGEVVV 119
Query: 121 GADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLIL 180
GADSHTCT+GA GAFATG+GSTD+A ATG+ WF+VPET+ F G+LQPYVT KD+IL
Sbjct: 120 GADSHTCTHGAFGAFATGIGSTDMAHVFATGKLWFKVPETIYFNITGDLQPYVTSKDVIL 179
Query: 181 HTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKE 240
IG++GV+GA YK +F G ++++S+ RMTM+NMAIE G KTGIIEPD+KT+ YVKE
Sbjct: 180 SIIGEVGVDGATYKACQFGGETVKKMSIASRMTMTNMAIEMGGKTGIIEPDEKTIQYVKE 239
Query: 241 RAK-----RKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKID 295
K R F+V K DEDA++ +V E + EPV A PH +N + A ID
Sbjct: 240 AMKKHGTERPFEVIKGDEDAEFAEVYEIEADKIEPVFACPHNVDNVKQAREVA--GKPID 297
Query: 296 QVFIGSCTNGRIEDLRMAAEILEGQ-KVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAG 354
QVFIGSCTNGR+EDLRMA +I+E +A VR++V P S Y KALKEG+IE FL+ G
Sbjct: 298 QVFIGSCTNGRLEDLRMAIKIIEKHGGIADDVRVVVTPASREEYLKALKEGIIEKFLKYG 357
Query: 355 AVIGPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGR 414
V+ P+C C+G GVL GE VST+NRNF GR G ++E+YLA+P AAA AV G
Sbjct: 358 CVVTNPSCSACMGSLYGVLGPGEVCVSTSNRNFRGRQGSLEAEIYLASPITAAACAVKGE 417
Query: 415 IASPEEV 421
+ P ++
Sbjct: 418 LVDPRDL 424
>sp|O27668|LE22_METTH PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7450349|pir||A69085 3-isopropylmalate dehydratase,
LeuC subunit - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2622758|gb|AAB86104.1| (AE000922)
3-isopropylmalate dehydratase, LeuC subunit
[Methanobacterium thermoautotrophicum]
Length = 428
Score = 429 bits (1091), Expect = e-119
Identities = 216/422 (51%), Positives = 295/422 (69%), Gaps = 7/422 (1%)
Query: 3 MTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFREL----GVKRVFDRE 58
M + EKILA+ + EV PGEI+ A++D +D T P I+ FR++ G RV+D E
Sbjct: 4 MNMTEKILAEAAGLREVTPGEIIEARVDLAMTHDGTSPPTIRTFRDIASRGGPARVWDPE 63
Query: 59 RIAIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDL 118
RI +V DH P I +AE + +REFA+E GI F+ + G+ H +LPE G V PG +
Sbjct: 64 RIVMVFDHNVPANTIGAAEFQRVTREFAREQGIVNIFQNAA-GICHQVLPERGFVRPGMV 122
Query: 119 IIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDL 178
I+GADSHTCTYGA GAFATG+G+TD+A+ ATG+ WF VPE M+ GE + +V KD+
Sbjct: 123 IVGADSHTCTYGAFGAFATGMGATDMAMVFATGKTWFMVPEAMRIEVTGEPEGHVYAKDV 182
Query: 179 ILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYV 238
ILH IG+IGV+GA Y+ +EF+G IE + V RMT+ NMA+E GAK GI+EP+++TLDYV
Sbjct: 183 ILHIIGEIGVDGATYRSVEFTGDTIESMDVSGRMTICNMAVEMGAKNGIMEPNRQTLDYV 242
Query: 239 KERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQVF 298
+ R R+F+VY SDED++Y + +DV++ EP VA P +N P+ + ID+ F
Sbjct: 243 RARTGREFRVYSSDEDSQYLEDHHFDVSDLEPQVACPDDVDNVYPVHRVE--GTHIDEAF 300
Query: 299 IGSCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIG 358
+GSCTNGR EDL++AAE++ ++V + VR IV P S +Y KAL++G+IE F+ AGA++
Sbjct: 301 LGSCTNGRYEDLKIAAEVIGDRRVHEDVRFIVSPASREIYLKALEDGIIETFIRAGAIVC 360
Query: 359 PPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASP 418
P CGPCLG HMGVLA GE +++TTNRNF GRMG P S VYLANP V A SA+ G I++P
Sbjct: 361 NPGCGPCLGAHMGVLAPGEVSIATTNRNFRGRMGDPASSVYLANPAVVAESAIEGVISAP 420
Query: 419 EE 420
++
Sbjct: 421 QQ 422
>gi|11499545 aconitase (acn) [Archaeoglobus fulgidus]
>gi|3122334|sp|O28316|LE21_ARCFU PUTATIVE
3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7450351|pir||B69495 aconitase (acn) homolog -
Archaeoglobus fulgidus >gi|2648576|gb|AAB89290.1|
(AE000967) aconitase (acn) [Archaeoglobus fulgidus]
Length = 433
Score = 418 bits (1064), Expect = e-116
Identities = 218/424 (51%), Positives = 296/424 (69%), Gaps = 10/424 (2%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFREL--GVKRVFDRE 58
M T+ EKI + S +E VK GE VMA +D +D+T PLAIK FRE+ +V+D+
Sbjct: 16 MAQTLVEKIFSKASGKE-VKAGEFVMANIDLAMIHDITAPLAIKAFREILGSDAKVWDKS 74
Query: 59 RIAIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDL 118
++ + DH P + +AE K R+FA+E GI + G G+ H ++ E V PG L
Sbjct: 75 KVIMAFDHQVPADSVHAAENHKMLRKFAEEQGILNYDVKG--GIAHQIMVE-NHVEPGML 131
Query: 119 IIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDL 178
I+GADSHTC YGALGAFATG+GSTD+ +A G+ WF+VPE+++F G+L+ +V GKD+
Sbjct: 132 IVGADSHTCMYGALGAFATGIGSTDMGFVLAMGKLWFKVPESIRFNVHGKLEKHVYGKDI 191
Query: 179 ILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYV 238
+L IG +G +GA YK +SG V+E+L + R+TM NMAIE G K GI+EPDK TL+Y+
Sbjct: 192 VLKLIGMVGADGANYKACIYSGEVVEKLGMSDRLTMCNMAIEMGGKAGIVEPDKTTLEYL 251
Query: 239 KERAK-RKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQV 297
K + + ++ KSDEDA++ +V E DVT EP VA PH +N V IS+ ++DQV
Sbjct: 252 KAMGRPYEGELLKSDEDAEFQEV-ELDVTGMEPQVAAPHRVDNVVGISEVE--GTRVDQV 308
Query: 298 FIGSCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVI 357
FIGSCTNGR EDL++AAEIL+G+KVA VRLIVIP S Y +ALKEGLIEIF++AGA++
Sbjct: 309 FIGSCTNGRYEDLKIAAEILKGEKVASNVRLIVIPASHREYRRALKEGLIEIFVDAGALV 368
Query: 358 GPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIAS 417
P CGPC+GG G++ASGE +VST+NRNF+GR G P+ ++YL NP VAAA+A+ G I
Sbjct: 369 EAPCCGPCMGGSFGLIASGEVSVSTSNRNFIGRQGSPEGKIYLVNPAVAAATAIYGEITD 428
Query: 418 PEEV 421
P ++
Sbjct: 429 PRKI 432
>pir||C72394 hypothetical protein TM0291 - Thermotoga maritima (strain MSB8)
>gi|4980790|gb|AAD35379.1|AE001711_2 (AE001711)
3-isopropylmalate dehydratase, large subunit, putative
[Thermotoga maritima]
Length = 418
Score = 400 bits (1017), Expect = e-110
Identities = 210/423 (49%), Positives = 281/423 (65%), Gaps = 6/423 (1%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
MG T+AEKI ++H R+ VK GEIV+A++D D T PL I +FRELG K V +
Sbjct: 1 MGKTLAEKIFSEHVGRD-VKAGEIVLARVDIAMAQDGTGPLMINEFRELGFKEV-KVPKA 58
Query: 61 AIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLII 120
+ +DH +P+ + + K REF KEMG+K F G G+ H +L E V PGDL+
Sbjct: 59 FLFIDHASPSPRKELSNSQKMMREFGKEMGVKVFDAGD--GISHQILAEK-YVKPGDLVA 115
Query: 121 GADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLIL 180
GADSHTCT G LGAF TG+GSTD+A+ G+ WF+VPET+K + G+LQ V KD+IL
Sbjct: 116 GADSHTCTAGGLGAFGTGMGSTDVAIIFGLGQNWFKVPETIKVVVNGKLQDGVYAKDIIL 175
Query: 181 HTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKE 240
+G +GA YK +EF GS IE ++VE R+T+SNMA+E GAK G++ D+KT +++K+
Sbjct: 176 EIARILGSDGATYKALEFHGSCIENMNVEDRLTISNMAVEVGAKAGLMPSDEKTREFLKK 235
Query: 241 RAKRK-FKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQVFI 299
+ + F+ K+D DA Y IE D T EP+V+ PH +N +S+ K IKIDQVFI
Sbjct: 236 MGREEDFRELKADPDAVYETEIEIDATTLEPLVSLPHYVDNVRKVSEVEKEKIKIDQVFI 295
Query: 300 GSCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGP 359
G+CTNGR++DL +A +ILE VRLIV P S VY AL++G+I+ F+E GA + P
Sbjct: 296 GTCTNGRLQDLEIALKILEKHGKHPDVRLIVGPASRKVYMDALEKGIIKKFVELGAAVIP 355
Query: 360 PTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPE 419
P CGPC+G HMGVL GER +ST NRNF GRMG+P +E+YLA+P AAA+AV G I P
Sbjct: 356 PGCGPCVGIHMGVLGDGERVLSTQNRNFKGRMGNPNAEIYLASPATAAATAVTGYITDPR 415
Query: 420 EVV 422
+
Sbjct: 416 RFI 418
>pir||H75354 3-isopropylmalate dehydratase, large subunit - Deinococcus
radiodurans (strain R1)
>gi|6459551|gb|AAF11331.1|AE002019_4 (AE002019)
3-isopropylmalate dehydratase, large subunit
[Deinococcus radiodurans]
Length = 431
Score = 399 bits (1013), Expect = e-110
Identities = 199/420 (47%), Positives = 280/420 (66%), Gaps = 5/420 (1%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
MGMTIAEK+LA HS + V PG+++ D+V +++T P A++ E G+ RVFD ++I
Sbjct: 1 MGMTIAEKMLAAHSGHDTVVPGQLIECATDWVLCHEITTPAALRMLEERGMDRVFDPQKI 60
Query: 61 AIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLII 120
V DH P +IK+A+ + + + +E GI+ F++ G G+ H +L GL+ PG ++
Sbjct: 61 VAVPDHSVPAMNIKAAKMYQKLKSWVQEKGIEHFYDVGRGGIAHVVLENTGLMKPGQTLV 120
Query: 121 GADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLIL 180
DSHTC GALGAFATGVGSTDLA A+ G+ WF+VPETM G+ P VT KD++L
Sbjct: 121 SGDSHTCNAGALGAFATGVGSTDLAGAIYAGKVWFKVPETMLIRVTGKTNPGVTPKDIVL 180
Query: 181 HTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKE 240
I IG +GA Y VME+ G I++L +E R T++NMAIEAG KTGI+ D T Y+++
Sbjct: 181 EVIKQIGADGANYMVMEWVGDYIDQLDMEGRFTLTNMAIEAGGKTGIVAVDDTTRAYMQQ 240
Query: 241 R--AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQVF 298
R + ++ Y+SD DAKY V+E D EP VA+PH+P N A I++ +
Sbjct: 241 RGVSPEQYTEYQSDPDAKYKVVVEIDAAQVEPTVAYPHIPSNG---RVAGSDRIRVTHAY 297
Query: 299 IGSCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIG 358
+GSCTNGRI DLR A IL+G+KVA+ V++IV+P + ++ +A +EGL+EIF++AGA +
Sbjct: 298 VGSCTNGRITDLRDVARILKGRKVAQDVQMIVVPATQAIWKQAAQEGLLEIFVDAGASVS 357
Query: 359 PPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASP 418
P+CG CLG H GVL + +S++NRNFVGRMG P +++YLA+P AASAV G IA P
Sbjct: 358 YPSCGACLGMHSGVLGPDDICISSSNRNFVGRMGDPSAQIYLASPATVAASAVEGFIADP 417
>gi|11499781 3-isopropylmalate dehydratase, large subunit (leuC) [Archaeoglobus
fulgidus] >gi|3122333|sp|O28084|LE22_ARCFU PUTATIVE
3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7450353|pir||G69524 3-isopropylmalate dehydratase,
large subunit (leuC) homolog - Archaeoglobus fulgidus
>gi|2648330|gb|AAB89057.1| (AE000954) 3-isopropylmalate
dehydratase, large subunit (leuC) [Archaeoglobus
fulgidus]
Length = 416
Score = 381 bits (969), Expect = e-105
Identities = 204/421 (48%), Positives = 284/421 (67%), Gaps = 8/421 (1%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
MG TIAEKIL++ S + + G+IV+A++D + D T PLAI++ ELG + V +R
Sbjct: 1 MGKTIAEKILSEKS-KSDAYAGDIVVAEIDQIALQDGTAPLAIRQLMELGTE-VRAADRT 58
Query: 61 AIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLII 120
+DH P+ + + K EFAK++G F G+ H ++ E V PGDL +
Sbjct: 59 HFFVDHAAPSPRRELSNDQKFIYEFAKKVGAD--FNPPGEGIIHQIMVER-YVKPGDLAV 115
Query: 121 GADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLIL 180
GADSHTCTYG +GAF+TG+GSTD+AVA+A G+ WFRVPE+ + +G L V KD+IL
Sbjct: 116 GADSHTCTYGGIGAFSTGMGSTDVAVAIALGKNWFRVPESFRVQLDGSLPKGVFAKDVIL 175
Query: 181 HTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKE 240
IGD+GV+GA YK +EF G E ++VE+R+T++NMA+E GAK GI E D+ T ++ E
Sbjct: 176 KLIGDLGVDGATYKALEFHGECAENMTVEERLTIANMAVECGAKAGIFESDENTRKFLAE 235
Query: 241 RAKR-KFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQVFI 299
+ F+ K+DEDA+Y K I DV++ PVV+ PH +N IS+ +++QV+I
Sbjct: 236 LGREGDFREVKADEDAEYEKEIYMDVSSLVPVVSKPHNVDNVAEISEVE--GTEVNQVYI 293
Query: 300 GSCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGP 359
G+CTNGR+ DL +AA IL+G+KV + VRLIV+P S VY +AL +GLI +F+EAG ++
Sbjct: 294 GTCTNGRLSDLEVAARILKGRKVKEGVRLIVVPASRRVYLQALDKGLIRVFVEAGGMVLN 353
Query: 360 PTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPE 419
P CGPC+G H G+LA GE +ST NRNF GRMG+P +E++LA+P AAASAV G IA P
Sbjct: 354 PGCGPCVGIHQGILADGEVCISTQNRNFKGRMGNPNAEIFLASPATAAASAVKGYIADPR 413
Query: 420 E 420
E
Sbjct: 414 E 414
>sp|Q58409|LE21_METJA PUTATIVE 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT
(ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|2127973|pir||B64425 homoaconitate hydratase (EC
4.2.1.36) - Methanococcus jannaschii
>gi|1591664|gb|AAB99007.1| (U67543) 3-isopropylmalate
dehydratase (leuC) [Methanococcus jannaschii]
Length = 420
Score = 365 bits (926), Expect = e-100
Identities = 200/424 (47%), Positives = 276/424 (64%), Gaps = 16/424 (3%)
Query: 3 MTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIAI 62
MT+ EKIL+ EV G+ + ++D +D T PLA K +E+ V++ ++I +
Sbjct: 1 MTLVEKILSK-KVGYEVCAGDSIEVEVDLAMTHDGTTPLAYKALKEMS-DSVWNPDKIVV 58
Query: 63 VLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLIIGA 122
DH P +K+AE K + EF K GIK F +GG G+ H +L E VLP + G
Sbjct: 59 AFDHNVPPNTVKAAEMQKLALEFVKRFGIKNFHKGGE-GICHQILAE-NYVLPNMFVAGG 116
Query: 123 DSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLILHT 182
DSHTCT+GA GAFATG G+TD+A ATGE W +VP+T++ G+ + V+ KD++L
Sbjct: 117 DSHTCTHGAFGAFATGFGATDMAYIYATGETWIKVPKTIRVDIVGKNEN-VSAKDIVLRV 175
Query: 183 IGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKER- 241
+IG GA Y +E+ G V++ + ++ R+T+ NMAIE G KTG+IE D+ T DY+K+
Sbjct: 176 CKEIGRRGATYMAIEYGGEVVKNMDMDGRLTLCNMAIEMGGKTGVIEADEITYDYLKKER 235
Query: 242 -------AK-RKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIK 293
AK +K ++ + ++A YYK IE D+T+ E VA PH P+N PIS +
Sbjct: 236 GLSDEDIAKLKKERITVNRDEANYYKEIEIDITDMEEQVAVPHHPDNVKPISDVE--GTE 293
Query: 294 IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEA 353
I+QVFIGSCTNGR+ DLR AA+ L+G++V K V+LIVIP S V+ +ALKEG+I+IF++A
Sbjct: 294 INQVFIGSCTNGRLSDLREAAKYLKGREVHKDVKLIVIPASKKVFLQALKEGIIDIFVKA 353
Query: 354 GAVIGPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLG 413
GA+I P CGPCLG H GVLA GE +STTNRNF GRMGH S +YLA+P +AA SAV G
Sbjct: 354 GAMICTPGCGPCLGAHQGVLAEGEICLSTTNRNFKGRMGHINSYIYLASPKIAAISAVKG 413
Query: 414 RIAS 417
I +
Sbjct: 414 YITN 417
>dbj|BAA74762.1| (AB017109) HacA [Thermus thermophilus]
Length = 418
Score = 337 bits (856), Expect = 1e-91
Identities = 190/424 (44%), Positives = 266/424 (61%), Gaps = 16/424 (3%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
MG T+AEKIL+ H V+ GE+V+ ++D V D K+ L + ER+
Sbjct: 1 MGQTLAEKILS-HKVGRPVRAGELVVVEVDQVMVVDSIAGSFFKRLEYLEATPRYP-ERV 58
Query: 61 AIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLII 120
+IV+DH P +++ A+ K RE+ K GI+ F G GV H +L E GL PG +++
Sbjct: 59 SIVIDHVAPAANLEVAKAQKEIREWGKRHGIRVFDVGR--GVCHQVLIEEGLAQPGWVVV 116
Query: 121 GADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLIL 180
G+DSH+ TYGA+GAF TG+G+TD+A+A A+G W RVPE++K ++ G L VT KD L
Sbjct: 117 GSDSHSTTYGAVGAFGTGMGATDIALAAASGRTWLRVPESVKVVFRGRLPKGVTAKDAAL 176
Query: 181 HTIGDIGVNGALYKVMEF---SGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDY 237
+ + GA Y +E G+ E L+ +RMT++N+ +EAGAK G++ P + L+
Sbjct: 177 EMVRLLTAEGATYMAVEIHLLDGA--EALTRGERMTLANLTVEAGAKAGLVVPSGEILEM 234
Query: 238 VKERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQV 297
R D DA+Y K +E D++ P V+ P +N +++ ++DQV
Sbjct: 235 Y-----RVPDWLYPDPDARYAKEVEIDLSALTPRVSVPFYVDNVHEVAQVKGK--RVDQV 287
Query: 298 FIGSCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVI 357
FIG+CTNGRIEDLR AAE+L G+KVA WVRL+V+P S V +A ++G + LEAGA I
Sbjct: 288 FIGTCTNGRIEDLRAAAEVLRGRKVAPWVRLLVVPASSQVLEEAARDGTLLTLLEAGATI 347
Query: 358 GPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIAS 417
G P CGPC+G HMGVLA GE VST+NRNF GRMG P +E+YLA+P VAAASAV G + +
Sbjct: 348 GTPGCGPCMGRHMGVLAPGEVCVSTSNRNFRGRMGAPDAEIYLASPRVAAASAVAGYLTT 407
Query: 418 PEEV 421
PEE+
Sbjct: 408 PEEL 411
>pir||T06300 hypothetical protein T9E8.170 - Arabidopsis thaliana
>gi|4584548|emb|CAB40778.1| (AL049608) putative protein
[Arabidopsis thaliana] >gi|7268046|emb|CAB78385.1|
(AL161536) putative protein [Arabidopsis thaliana]
Length = 509
Score = 323 bits (820), Expect = 2e-87
Identities = 189/448 (42%), Positives = 264/448 (58%), Gaps = 30/448 (6%)
Query: 2 GMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKF-RELGVK-RVFDRER 59
GMT+ EKILA SE+ V PG+ + +D + +DV P A F RE G K +V+D E+
Sbjct: 66 GMTMTEKILARASEKSLVVPGDNIWVNVDVLMTHDVCGPGAFGIFKREFGEKAKVWDPEK 125
Query: 60 IAIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVG----------VEHCLLPE 109
I ++ DH+ D ++ RE +E IK+F++ +G V H L +
Sbjct: 126 IVVIPDHYIFTADKRANRNVDIMREHCREQNIKYFYDITDLGNFKANPDYKGVCHVALAQ 185
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGEL 169
G PG++++G DSHTCT GA G FATG+G+TD + TG+ +VP TM+FI +GE+
Sbjct: 186 EGHCRPGEVLLGTDSHTCTAGAFGQFATGIGNTDAGFVLGTGKILLKVPPTMRFILDGEM 245
Query: 170 QPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIE 229
Y+ KDLIL IG+I V GA YK MEFSG+ IE LS+E+RMT+ NM +EAG K G+I
Sbjct: 246 PSYLQAKDLILQIIGEISVAGATYKTMEFSGTTIESLSMEERMTLCNMVVEAGGKNGVIP 305
Query: 230 PDKKTLDYVKERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAK 289
PD TL+YV+ F SD +A + +DV+ EPVVA PH P+N +++ K
Sbjct: 306 PDATTLNYVEACILSCFLPVYSDGNASFVADYRFDVSKLEPVVAKPHSPDNRA-LARECK 364
Query: 290 MNIKIDQVFIGSCTNGRIEDLRMAAEILE--GQKVAKWVRLIVIPCSPTVYWK------- 340
++KID+V+IGSCT G+ ED AA++ G+KV V ++P + V+
Sbjct: 365 -DVKIDRVYIGSCTGGKTEDFMAAAKLFHAAGRKVK--VPTFLVPATQKVWMDVYALPVP 421
Query: 341 -ALKEGLIEIFLEAGA-VIGPPTCGPCLGGHMGVLA---SGERAVSTTNRNFVGRMGHPK 395
A + +IF EAG P+CG CLGG A + VSTTNRNF GRMGH +
Sbjct: 422 GAGGKTCAQIFEEAGCDTPASPSCGACLGGPADTYARLNEPQVCVSTTNRNFPGRMGHKE 481
Query: 396 SEVYLANPYVAAASAVLGRIASPEEVVK 423
++YLA+PY AAASA+ GR+A P E ++
Sbjct: 482 GQIYLASPYTAAASALTGRVADPREFLQ 509
>pir||D75373 3-isopropylmalate dehydratase, large subunit - Deinococcus
radiodurans (strain R1)
>gi|6459378|gb|AAF11172.1|AE002005_1 (AE002005)
3-isopropylmalate dehydratase, large subunit
[Deinococcus radiodurans]
Length = 434
Score = 314 bits (797), Expect = 9e-85
Identities = 175/422 (41%), Positives = 265/422 (62%), Gaps = 14/422 (3%)
Query: 4 TIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKF-RELGVKRVFDRERIAI 62
T+AEKIL+ V G++ + ++D V D I++ R+LG + ER++I
Sbjct: 20 TVAEKILSQRGSAA-VYAGDLAVVEVDQVMVVDSIAQSFIERMERDLGAVPKYP-ERVSI 77
Query: 63 VLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLIIGA 122
V+DH P + A+ K +RE+A + G++ F G G+ H +L E L PG +++G+
Sbjct: 78 VVDHVAPASTVSVAQAQKEAREYAAKTGVRLFDVGR--GICHQVLMEEKLAQPGWIVLGS 135
Query: 123 DSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLILHT 182
DSH+ TYGA+ AF +G+G+TD+A+A A+G+ W RVPE++K G+L+P VT KD+ L
Sbjct: 136 DSHSTTYGAVAAFGSGMGATDIALAAASGKTWLRVPESVKVTLTGDLRPGVTAKDVALEM 195
Query: 183 IGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTL-DYVKER 241
I +G +GA Y+ +E + + +RMT++N+ +EAGAK G++ P + L DY +
Sbjct: 196 IRVLGADGATYQSVEIHAG--DRFTRGERMTLANLCVEAGAKAGLVVPGGEILTDYGYDV 253
Query: 242 AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQVFIGS 301
+ DE A Y + +E D++ P ++ P +N +++ +K+DQVFIG+
Sbjct: 254 PAWVYP----DEGAAYAREVEIDLSALHPRMSAPSEVDNVHDVAELR--GLKVDQVFIGT 307
Query: 302 CTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPT 361
CTNGRIEDL AAE+L G++V RL+VIP S V +AL++G + AGAV+G P
Sbjct: 308 CTNGRIEDLHAAAEVLRGRRVDPTTRLLVIPASSQVMEEALQDGTLLTLQRAGAVLGTPG 367
Query: 362 CGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEEV 421
CGPC+G H GVLA GE VST+NRNF+GRMG + +YLA+P VAAA+AV+GR+A PE+V
Sbjct: 368 CGPCMGRHQGVLAPGEVCVSTSNRNFIGRMGDKDAHIYLASPAVAAATAVMGRVALPEDV 427
Query: 422 VK 423
+
Sbjct: 428 AQ 429
>pir||A71181 probable isomerase - Pyrococcus horikoshii
>gi|3258157|dbj|BAA30840.1| (AP000007) 380aa long
hypothetical isomerase [Pyrococcus horikoshii]
Length = 380
Score = 296 bits (751), Expect = 2e-79
Identities = 172/418 (41%), Positives = 244/418 (58%), Gaps = 44/418 (10%)
Query: 3 MTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFD--RERI 60
MT+ E+IL + K GE+V+ +D V+ +D TMPL I+ F +VF+ + R
Sbjct: 1 MTLVEEIL-------DAKAGEVVIRNVDLVYAHDGTMPLIIEAFN-----KVFNSVKARA 48
Query: 61 AIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLII 120
+ DH P +K A K REFA+ GI EG G+ H L+ E+GL +++
Sbjct: 49 YVFFDHVYPAPTVKVANLQKEIREFARRHGIP-VVEGK--GISHQLVVEMGLAEKSRIVV 105
Query: 121 GADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLIL 180
G DSHT T GALG FA G+G+TD+AV + G+ W RVPE++ I+EG+ D+++
Sbjct: 106 GGDSHTPTLGALGVFAVGMGATDVAVVLGLGKTWLRVPESVAVIFEGKPSRTTMAADVMI 165
Query: 181 HTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKE 240
I + YK +EF + ++R+T++N ++EA AKT +I + +Y+KE
Sbjct: 166 RLITSLRNYDMNYKALEFFNV---PFTADERLTLTNFSVEANAKTALIGEEYDGRNYIKE 222
Query: 241 RAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQVFIG 300
I +++++ P+VA P P N V + K KIDQVFIG
Sbjct: 223 --------------------ISFELSSLGPMVAKPFSPANGVEVEKVE--GTKIDQVFIG 260
Query: 301 SCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPP 360
SCTNGR E ++ AAEIL+G++VA VR I+ P S VY + ++EG+++I L+AGA I PP
Sbjct: 261 SCTNGRFEHIKRAAEILKGEEVA--VRTIIGPASVNVYKRMVREGIVDILLDAGATILPP 318
Query: 361 TCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASP 418
CGPCLG HMGVL E +STTNRNF GRMG P +E+YLA+P AA SA+ G I SP
Sbjct: 319 GCGPCLGRHMGVLGDKEVVLSTTNRNFRGRMGSPTAEIYLASPLTAAVSALYGEITSP 376
>pir||H75159 3-isopropylmalate dehydratase, large chain (leuc-2) PAB0287 -
Pyrococcus abyssi (strain Orsay)
>gi|5457869|emb|CAB49359.1| (AJ248284) 3-isopropylmalate
dehydratase, large subunit (leuC-2) [Pyrococcus abyssi]
Length = 380
Score = 295 bits (748), Expect = 5e-79
Identities = 176/419 (42%), Positives = 241/419 (57%), Gaps = 44/419 (10%)
Query: 3 MTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFD--RERI 60
MT+ K+L KPGE V+ K+D V+ +D TMPL I+ F R+F+ R R
Sbjct: 1 MTLVGKLL-------NAKPGEAVIRKVDLVYAHDGTMPLIIEAFN-----RIFNEVRTRA 48
Query: 61 AIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLII 120
I DH P +K A + REFAK GI EG G+ H L+ ELGL +I+
Sbjct: 49 YIFFDHVYPAPTVKVANLHREIREFAKIHGIP-VVEGK--GISHQLVVELGLAEEAKIIV 105
Query: 121 GADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLIL 180
G DSHT T GALG FA G+G+TD+A+A+ G+ W +VPE++ + +G + Y+ D+++
Sbjct: 106 GGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMI 165
Query: 181 HTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKE 240
H I + Y +EF S ++R+T++N ++EA AKT II + + Y
Sbjct: 166 HLITSLKDREMSYNAIEFFNV---PFSFDERLTLTNFSVEANAKTAIIGEEYEGGGY--- 219
Query: 241 RAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQVFIG 300
SDE I ++ + P+VA P P N V + KIDQVFIG
Sbjct: 220 ----------SDE-------ITIELDSLPPMVAKPFSPANGVSVEDVE--GTKIDQVFIG 260
Query: 301 SCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPP 360
SCTNGR E ++ AAEIL+G++V VR +V P S VY + ++EGL+ I ++AG VI PP
Sbjct: 261 SCTNGRFEQIKRAAEILDGEQVN--VRTLVAPASINVYRRMIEEGLVRILIDAGVVILPP 318
Query: 361 TCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPE 419
CGPCLG HMGV E +STTNRNF GRMG PK+E+YLA+P AA SA+ G I +PE
Sbjct: 319 GCGPCLGRHMGVAGDNEVILSTTNRNFRGRMGSPKAEIYLASPVTAAVSALYGEITNPE 377
>gb|AAK42608.1| 3-isopropylmalate dehydratase, large subunit (isopropylmalate
isomerase) (alpha IPM isomerase) (IPMI) (leuC)
[Sulfolobus solfataricus]
Length = 416
Score = 291 bits (738), Expect = 8e-78
Identities = 163/425 (38%), Positives = 251/425 (58%), Gaps = 21/425 (4%)
Query: 4 TIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIAIV 63
T+ EKIL+ S ++ V PG+++ AK+D V +D+T I+ + + +VFD+ ++ I
Sbjct: 6 TLTEKILSRASGKD-VSPGDVIEAKVDLVAFHDLTGYHVIEVMEKANMIKVFDKSKLVIA 64
Query: 64 LDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLIIGAD 123
DH P D++SAE R+F K GI F++ + G+ H ++ E PG +I+ AD
Sbjct: 65 FDHLAPPPDVRSAEIQGYIRKFVKSEGIPNFYDI-NYGILHEVMIEQ-YANPGQVILAAD 122
Query: 124 SHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLILHTI 183
SHT T GA+GAFA G+G++D+A A+ TG+ W VP+ K + EG+ ++TGKD+ L +
Sbjct: 123 SHTTTSGAVGAFAQGMGASDIAAALITGKTWLVVPQPFKVVLEGKPAKWITGKDVALKLL 182
Query: 184 GDIGVNGALYKVMEFSGSVIE-------ELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLD 236
GD +K F+G +E ++ R T+SNM IE A + PD++T
Sbjct: 183 GD-------FKADYFNGMTLEIFVKDPLSFPMDYRATVSNMGIEMNADALMFIPDQETKR 235
Query: 237 YVKERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQ 296
Y+KE + ++ D AKY + EP+VA PH +N +++ +DQ
Sbjct: 236 YIKEMRGYEPELVTPDNGAKYVDEYTIQLDEMEPLVAAPHSVDNVKVVNELE--GTPVDQ 293
Query: 297 VFIGSCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAV 356
V+IGSCTNGR+ D +AA+I++G+KV R I IP S ++ +AL+ G I+ ++AG +
Sbjct: 294 VYIGSCTNGRLSDFEIAAKIMKGKKVKS--RCIAIPASYRMFKEALERGYIQTLVDAGCI 351
Query: 357 IGPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIA 416
+ TCGPCLGGH G+ GE VST++RNF GRMG +++VYL+ P VAA SA+ G+I
Sbjct: 352 VTYGTCGPCLGGHFGIAGPGENIVSTSSRNFKGRMGSNEAKVYLSGPAVAAISALEGKIT 411
Query: 417 SPEEV 421
P +
Sbjct: 412 DPRVI 416
>pir||T29083 3-isopropylmalate dehydratase (EC 4.2.1.33) large chain -
Streptomyces coelicolor >gi|3355701|emb|CAA20001.1|
(AL031124) 3-isopropylmalate dehydratase large subunit
[Streptomyces coelicolor A3(2)]
Length = 476
Score = 262 bits (663), Expect = 5e-69
Identities = 176/470 (37%), Positives = 245/470 (51%), Gaps = 54/470 (11%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
MG T+AEK+ DH R GE + +D ++VT P A R+ G R R +
Sbjct: 1 MGRTLAEKVWDDHVVRRA--EGEPDLLFIDLHLLHEVTSPQAFDGLRKSG--RPVRRLDL 56
Query: 61 AIVL-DHFTPNKDIK-------SAEQCKSSREFAKEMGIKWFFEGG-SVGVEHCLLPELG 111
I DH TP DI S Q ++ R+ + G++ G GV H + P+LG
Sbjct: 57 TIATEDHNTPTLDIDKPIADPVSRAQLETLRKNCADFGVRLHPLGDVEQGVVHVVGPQLG 116
Query: 112 LVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQP 171
L PG ++ DSHT T+GA GA A G+G++ + +AT P+TM +GEL
Sbjct: 117 LTQPGTTVVCGDSHTSTHGAFGALAFGIGTSQVEHVLATQTLPLARPKTMAITVDGELPE 176
Query: 172 YVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPD 231
VT KDLIL I IG G ++E+ G IE+LS+E RMT+ NM+IEAGA+ G+I PD
Sbjct: 177 GVTAKDLILAIIARIGTGGGQGYILEYRGEAIEKLSMEARMTICNMSIEAGARAGMIAPD 236
Query: 232 KKTLDYVKER-----------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ T Y++ R A +K ++D+DA++ + + P V + P
Sbjct: 237 ETTFAYLQGRPHAPEGADWDAAVEYWKTLRTDDDAEFDAEVVIEAAELAPFVTWGTNPGQ 296
Query: 281 TVPISKAAK-----------------------------MNIKIDQVFIGSCTNGRIEDLR 311
P+S A +I++D VF+GSCTNGRIEDLR
Sbjct: 297 GAPLSAAVPDPASYEDASERFAAEKALEYMGLEAGQPLRSIQVDTVFVGSCTNGRIEDLR 356
Query: 312 MAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMG 371
AAEI+ +KVA VR++V+P S V +A+ EGL +F EAGA C CLG +
Sbjct: 357 AAAEIVRDRKVADGVRMLVVPGSARVGLQAVSEGLDVVFKEAGAEWRHAGCSMCLGMNPD 416
Query: 372 VLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEEV 421
LA GER+ ST+NRNF GR G +L +P VAAA+AVLG +ASP ++
Sbjct: 417 QLAPGERSASTSNRNFEGRQG-KGGRTHLVSPQVAAATAVLGHLASPADL 465
>pir||T51171 homoaconitate hydratase (EC 4.2.1.36) [validated] - Thermus
aquaticus (fragment) >gi|3724368|dbj|BAA33786.1|
(AB018379) homoaconitate hydratase [Thermus
thermophilus]
Length = 386
Score = 257 bits (649), Expect = 2e-67
Identities = 162/417 (38%), Positives = 229/417 (54%), Gaps = 52/417 (12%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
+G T+AEKIL+ H V+ GE+V+ ++D V D K+ L + ER+
Sbjct: 3 VGQTLAEKILS-HKVGRPVRAGELVVVEVDQVMVVDSIAGSFFKRLEYLEATPRYP-ERV 60
Query: 61 AIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLII 120
+IV+DH P +++ A+ K RE+ K GI+ F G GV H +L E GL PG +++
Sbjct: 61 SIVIDHVAPAANLEVAKAQKEIREWGKRHGIRVFDVGR--GVCHQVLIEEGLAQPGWVVV 118
Query: 121 GADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDL-- 178
G+DSH+ TYGA+GAF TG+G+TD+A+A A+G W RVPE++K ++ G L V G+
Sbjct: 119 GSDSHSTTYGAVGAFGTGMGATDIALAAASGRTWLRVPESVKVVFRGRLPKGVHGQGRPP 178
Query: 179 -----ILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKK 233
+LH GA + R T+ ++ G +
Sbjct: 179 WRWVPLLHR-----PEGAT--------------TWRWRSTLRRATLQRG--------QRM 211
Query: 234 TLDYVKERAKRK------------FKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENT 281
T ERAKR ++ S Y K +E D++ P V+ P +N
Sbjct: 212 TSQLTVERAKRARVALGRSWRCTGARLALSRPRCPYAKEVEIDLSALTPRVSVPFYVDNV 271
Query: 282 VPISKAAKMNIKIDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKA 341
+++ ++DQVFIG+CTNGRIEDLR AAE+L G+KVA WVRL+V+P S V +A
Sbjct: 272 HEVAQVKGK--RVDQVFIGTCTNGRIEDLRAAAEVLRGRKVAPWVRLLVVPASSQVLEEA 329
Query: 342 LKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEV 398
++G + LEAGA IG P CGPC+G HMGVLA GE VST+NRNF GRMG P +E+
Sbjct: 330 ARDGTLLTLLEAGATIGTPGCGPCMGRHMGVLAPGEVCVSTSNRNFRGRMGAPDAEI 386
>gb|AAG20620.1| (AE005132) aconitase; Can [Halobacterium sp. NRC-1]
Length = 661
Score = 254 bits (643), Expect = 1e-66
Identities = 155/423 (36%), Positives = 228/423 (53%), Gaps = 10/423 (2%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
MG T+ EKIL DH +++PGE + ++D D T + +F L + V E
Sbjct: 1 MGQTLTEKILDDHLVEGDLQPGEEIGIEIDQTLSQDTTGTMVWLQFEALEMDEV-QTELA 59
Query: 61 AIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLII 120
A DH T D K+ + + R A G +F G+ H + E PG ++
Sbjct: 60 AQYCDHQTYQFDFKNTDDHRFLRSAAGTYGA--YFSRPGNGICHNVHKE-NFAAPGKTLL 116
Query: 121 GADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLIL 180
G+DSHT T G LG A G G D+AVAM G + +PE + EGEL + T KD+IL
Sbjct: 117 GSDSHTPTPGGLGQLAIGAGGIDIAVAMGGGAYYVEMPEVVNVRLEGELPEWATAKDVIL 176
Query: 181 HTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKE 240
+ + V G + KV+E++G +E LSV +R T++NM E GA + I D +T D++
Sbjct: 177 ELLRRLSVKGGVGKVLEYTGPGVESLSVPERTTITNMGTELGATSSIFPTDDRTEDWLAR 236
Query: 241 RAKR-KFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQVFI 299
+ +F ++D DA+Y I D+++ EP++A P +P+N VP+ + A +++QV +
Sbjct: 237 FDREDEFVDLQADADAEYADEIVVDLSDLEPLIAEPSMPDNVVPVREVAGQ--EVEQVMV 294
Query: 300 GSCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGP 359
GSCTNG ED+ AA++LEG++V+K +IV P S +EG + AG
Sbjct: 295 GSCTNGAYEDVLPAAKMLEGREVSKHTEMIVAPGSKQASEMLAREGWTAEMMAAGVNFSE 354
Query: 360 PTCGPCLG-GHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASP 418
TCG C+G GH V AS ++ T NRNF GR G VYL +P VA ASA+ G I P
Sbjct: 355 ATCGACIGIGH--VPASDSVSLRTFNRNFEGRSGIEDDSVYLCSPEVATASAIAGEIVDP 412
Query: 419 EEV 421
++
Sbjct: 413 RDL 415
>pir||T38665 probable homoaconitate hydratase (EC 4.2.1.36) - fission yeast
(Schizosaccharomyces pombe) >gi|5706517|emb|CAB52279.1|
(AL109739) putative homoaconitate hydratase (EC
4.2.1.36) [Schizosaccharomyces pombe]
Length = 721
Score = 252 bits (637), Expect = 5e-66
Identities = 151/436 (34%), Positives = 231/436 (52%), Gaps = 23/436 (5%)
Query: 4 TIAEKILADHS----EREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRER 59
TI EKI+ + E + V+ G+ V K +D + P+A+K F +G K+VFD +
Sbjct: 62 TITEKIVQKFAQNIPENKYVRSGDYVTIKPKHCMSHDNSWPVALK-FMGIGAKKVFDNRQ 120
Query: 60 IAIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLI 119
I LDH NK + + K+ FAK GI ++ G +G H ++ E G +PG +
Sbjct: 121 IVCTLDHDVQNKSEANLRKYKNIESFAKGQGIDFYPAGRGIG--HQIMVEQGYAMPGSMA 178
Query: 120 IGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLI 179
+ +DSH+ TYG +G T + TD A ATG+ W+++P + G+L ++GKD+I
Sbjct: 179 VASDSHSNTYGGVGCLGTPIVRTDAAAIWATGQTWWQIPPIARVNLVGQLPKGLSGKDII 238
Query: 180 LHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVK 239
+ G + L +EF G + LS+E R+T++NM E GA +G+ D+K L + +
Sbjct: 239 VSLCGAFNHDEVLNHAIEFYGEGLNSLSIESRLTIANMTTEWGALSGLFPTDEKLLAWYE 298
Query: 240 ERAKR----------------KFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVP 283
+R K K +DE A Y K + D++ P V+ P+ +
Sbjct: 299 DRLKFLGPNHPRVNRETLDAIKASPILADEGAFYAKHLILDLSTLSPAVSGPNSVKVYNS 358
Query: 284 ISKAAKMNIKIDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALK 343
+ K +I I + ++ SCTNGR+ D+ AAE ++G+KVA V V S V A K
Sbjct: 359 AATLEKKDILIKKAYLVSCTNGRLSDIHDAAETVKGKKVADGVEFYVGAASSEVEAAAQK 418
Query: 344 EGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANP 403
G + +++GA P CGPC+G G+L GE +S TNRNF GRMG ++ YLA+P
Sbjct: 419 NGDWQTLIDSGARTLPAGCGPCIGLGTGLLKDGEVGISATNRNFKGRMGSREALAYLASP 478
Query: 404 YVAAASAVLGRIASPE 419
V AASA+ G+I +PE
Sbjct: 479 AVVAASAIAGKIVAPE 494
>emb|CAC14578.1| (AJ296268) 2-isopropylmalate isomerase large subunit [Sinorhizobium
meliloti]
Length = 448
Score = 249 bits (629), Expect = 5e-65
Identities = 159/435 (36%), Positives = 227/435 (51%), Gaps = 51/435 (11%)
Query: 35 NDVTMPLAIKKFRELGVKRVFDRERIAIVLDHFTPN--------KDIKSAEQCKSSREFA 86
++VT P A + R G ++V E+ V+DH P K+ +S Q ++ A
Sbjct: 14 HEVTSPQAFEGLRMAG-RKVRAPEKTLAVVDHNVPTSPDRHLGIKNEESRIQVEALARNA 72
Query: 87 KEMGIKWFFEGGS-VGVEHCLLPELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLA 145
++G++++ E G+ H + PE G LPG I+ DSHT T+GA GA A G+G++++
Sbjct: 73 ADLGVEYYSENDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVE 132
Query: 146 VAMATGEAWFRVPETMKFIYEGELQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEE 205
+AT + + M +G+L P VT KD+IL IG+IG G V+EF+G I
Sbjct: 133 HVLATQTLIQKKAKNMLVRVDGQLPPGVTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRS 192
Query: 206 LSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVK-----------ERAKRKFKVYKSDED 254
LS+E RMT+ NM IE GA+ G+I PD+ T +Y+K +RA +K DE
Sbjct: 193 LSMEGRMTVCNMTIEGGARAGLIAPDETTFEYIKDKPRAPKGEAWDRAVEYWKTLHMDEG 252
Query: 255 AKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAK------------------------- 289
A Y +V+ D N P+V++ PE+ V +
Sbjct: 253 AHYDRVVVLDAANLPPIVSWGSSPEDVVSVQGVVPNPDDIQDETKRTSKWRALDYMGLKP 312
Query: 290 ----MNIKIDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEG 345
+I ID+VFIGSCTNGRIEDLR AE++EG+KV+ V +++P S V +A EG
Sbjct: 313 GTKITDIAIDRVFIGSCTNGRIEDLRAVAEVVEGRKVSPTVSAMIVPGSGLVKEQAEAEG 372
Query: 346 LIEIFLEAGAVIGPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYV 405
L +IF EAG P C CL + L GER ST+NRNF GR G K +L +P +
Sbjct: 373 LDKIFKEAGFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGF-KGRTHLLSPAM 431
Query: 406 AAASAVLGRIASPEE 420
AAA+AV G E
Sbjct: 432 AAAAAVAGHFVDIRE 446
>dbj|BAA37138.1| (AB017135) isopropylmalate isomerase large subunit [Thermus
thermophilus]
Length = 472
Score = 247 bits (625), Expect = 1e-64
Identities = 163/468 (34%), Positives = 233/468 (48%), Gaps = 50/468 (10%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
MG T+ EK+ H R+ +K G+ + +D ++VT P A ++LG++ +
Sbjct: 1 MGKTLYEKVWEAHEVRK-LKNGQSQLF-IDLHLLHEVTSPQAFGMLKDLGLRVRYPHRTF 58
Query: 61 AIVLDHFTPNKDIKSAEQ-------CKSSREFAKEMGIKWFFEG-GSVGVEHCLLPELGL 112
A V DH P D Q ++ R +E GI +F G G+ G+ H + P+LGL
Sbjct: 59 ATV-DHIVPTHDRTEPFQDPLAQSMLEALRANTREHGITFFDLGSGNQGIVHVIGPQLGL 117
Query: 113 VLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPY 172
PG I DSHT T+GA GA A G+G++ + +AT + + + EG L P
Sbjct: 118 TQPGMTIACGDSHTSTHGAFGAVAFGIGTSQVRDVLATQTLAAQKLKVRRINVEGRLAPG 177
Query: 173 VTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDK 232
V KD+ILH I +GV G L E+ GS +E + +E RMT+ NM+IE GA+ G + PD+
Sbjct: 178 VYAKDVILHIIRHLGVKGGLGYAYEYGGSAVEAMDMESRMTLCNMSIEGGARIGYVNPDE 237
Query: 233 KTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAF------- 274
T Y++ E AKR++ ++SD DA Y V+ + P V +
Sbjct: 238 TTFQYLEGRPYVPKGSEWEEAKRRWLAWRSDPDASYDDVVTFRAEEIAPTVTWGITPGQA 297
Query: 275 ----------PHLPENTVPISKAAKM-----------NIKIDQVFIGSCTNGRIEDLRMA 313
LPE P+++ A + I FIGSCTN R+ DLR
Sbjct: 298 IPIDGRIPLLEELPEEERPVAEEALAYMGFRPGQPIKGVPIQVAFIGSCTNARLSDLREV 357
Query: 314 AEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVL 373
A L+G KV K VR +V+P S V KA +EG+ E+F EAG P C CL + L
Sbjct: 358 ARYLKGHKVKKGVRALVVPGSEWVARKAEEEGIAEVFREAGFEWRMPGCSMCLAMNPDRL 417
Query: 374 ASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEEV 421
E S++NRN+ GRMG P+ L +P + AA+AV G IA EV
Sbjct: 418 EGDELCASSSNRNYKGRMGSPRGRTVLMSPLMVAAAAVAGEIADAREV 465
>pir||F70453 aconitase - Aquifex aeolicus >gi|2984078|gb|AAC07617.1| (AE000756)
aconitase [Aquifex aeolicus]
Length = 659
Score = 246 bits (621), Expect = 4e-64
Identities = 150/421 (35%), Positives = 232/421 (54%), Gaps = 8/421 (1%)
Query: 4 TIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIAIV 63
T+A KI+ +H +++PGE + K+D D T + +F +GV V ++ +
Sbjct: 5 TVAWKIIKNHLVSGKMEPGEEIAIKIDQTLTQDATGTMCYLEFEAMGVPEVKTELSVSYI 64
Query: 64 LDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLIIGAD 123
DH D ++A+ K AK+ GI W + G+ G+ H + E PG ++G+D
Sbjct: 65 -DHNMLQTDFRNADDHKYLMSVAKKFGI-WLSKPGN-GICHQVHLER-FAKPGKTLLGSD 120
Query: 124 SHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLILHTI 183
SHT T G +G A G G D+A AMA + ++P+ + +G++ +VT KD+IL +
Sbjct: 121 SHTPTAGGMGMLAIGAGGLDVAAAMAGEPFYLKMPKIVGVHLKGKMPEWVTAKDIILELL 180
Query: 184 GDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKERAK 243
+ V G L K+ E+ G ++ELSV +R T++NM E GA T I D+ T Y++ + +
Sbjct: 181 RRLTVKGGLGKIFEYFGEGVKELSVPERATITNMGAELGATTSIFPSDEITRAYLRAQGR 240
Query: 244 RKFKV-YKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKMNIKIDQVFIGSC 302
+ V D DA+Y +VIE +++ EP++A PH P+N VP+ + IK+DQV IGSC
Sbjct: 241 EEDWVELLPDPDAEYDEVIEINLSELEPLIAQPHSPDNVVPVREIE--GIKVDQVVIGSC 298
Query: 303 TNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTC 362
TN DL AA++LEG++V V V P S + G + FL+AGA I C
Sbjct: 299 TNSSFVDLTRAAKLLEGRRVHPDVVFAVAPGSKQALELITQNGALLNFLKAGARILESAC 358
Query: 363 GPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEEVV 422
GPC+G +G +V + NRNF GR G P ++VYLA+P A+A+ G I P ++
Sbjct: 359 GPCIGMGFAPPTNGV-SVRSFNRNFKGRAGTPDAKVYLASPETCVAAAIAGEIIDPRKLA 417
Query: 423 K 423
+
Sbjct: 418 E 418
>gi|10957109 3-isopropylmalate dehydratase [Buchnera sp. APS]
>gi|7707823|dbj|BAA95425.1| (AP001071) 3-isopropylmalate
dehydratase [Buchnera sp. APS]
Length = 475
Score = 246 bits (621), Expect = 4e-64
Identities = 163/465 (35%), Positives = 251/465 (53%), Gaps = 58/465 (12%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ +KI H EE ++ L + ++VT P A R+ ++V ++
Sbjct: 5 MKKTLYDKIYDSHIVYEEKNNTSLLYIDLHLL--HEVTSPQAFDSLRDKN-RKVRQPKKT 61
Query: 61 AIVLDH--FTPNKDIKSA---------EQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPE 109
+DH T ++DI ++ E K+ EF + + + + G+ H + PE
Sbjct: 62 FATMDHNVSTTSQDINASGSMAKVQMQELIKNCSEFNISL---YDIKNPNQGIVHVISPE 118
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGEL 169
G+ LPG I+ DSHT T+GA GA + G+G++++ +AT + + MK GE+
Sbjct: 119 KGMTLPGMTIVCGDSHTSTHGAFGALSFGIGTSEVEHVLATQTLKQQRFKNMKIEITGEI 178
Query: 170 QPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIE 229
Q +VT KDLIL IG +G +G ++EF G+VIE++S+E+RMT+ NMAIE GAK+G+I
Sbjct: 179 QKFVTAKDLILFIIGKLGSSGGAGYIIEFCGNVIEKMSMEERMTICNMAIEIGAKSGLIA 238
Query: 230 PDKKTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLP 278
PD+ T Y+K ++A +K KSD++A + KV+ ++++ P + + P
Sbjct: 239 PDEVTFSYLKNKMYAPRGVFWKKALNFWKNLKSDKNAFFDKVVNINISDLSPQITWGTNP 298
Query: 279 ENTVPISK-------------------AAKM----------NIKIDQVFIGSCTNGRIED 309
+ + I + A K NI +D+VFIGSCTNGRIED
Sbjct: 299 DQVISIDQKIPDFSSFDNLIKKDLAKSACKYMGLKIGTYLTNITVDKVFIGSCTNGRIED 358
Query: 310 LRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGH 369
LR A++IL+ +K+A V+ IV+P S +V +A EGL +IF+ AG P C CLG +
Sbjct: 359 LRAASKILKDKKIANNVKAIVVPGSGSVKREAENEGLDKIFISAGFEWRLPGCSMCLGMN 418
Query: 370 MGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGR 414
L GER ST+NRNF GR G +L +P +AAA+AV G+
Sbjct: 419 KDRLNDGERCASTSNRNFEGRQGR-GGRTHLVSPIMAAAAAVYGK 462
>sp|P55251|LEU2_RHIPU 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMALATE ISOMERASE)
(ALPHA-IPM ISOMERASE) (IPMI) >gi|1000692|dbj|BAA11052.1|
(D67033) LeuA [Rhizomucor pusillus]
Length = 755
Score = 245 bits (619), Expect = 7e-64
Identities = 164/467 (35%), Positives = 240/467 (51%), Gaps = 58/467 (12%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
MG T+ +K+ DH ++ ++ V ++VT P A + R G ++V +
Sbjct: 1 MGRTLYDKVWDDHVVDQQEDGTCLIYIDRHLV--HEVTSPQAFEGLRNAG-RKVRRPDCT 57
Query: 61 AIVLDH---FTPNKDIK----------SAEQCKSSREFAKEMGIKWF-FEGGSVGVEHCL 106
+DH TP K+ K S QC++ KE G+ +F + G+ H +
Sbjct: 58 LATVDHNIPTTPRKNFKNVSTFIQEADSRTQCETLEHNVKEFGLTYFGMDDSRQGIVHVI 117
Query: 107 LPELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYE 166
PE G LPG + DSHT T+GA GA A G+G++++ +AT + + M+ +
Sbjct: 118 GPEQGFTLPGCTVNCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLLQKKSKNMRVKVD 177
Query: 167 GELQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTG 226
G+L P VT KD++LH IG IG G V+EF GS E++S+E RM++ NM+IEAGA+ G
Sbjct: 178 GKLAPGVTSKDIVLHIIGVIGTAGGTGCVIEFCGSAFEQMSMEARMSVCNMSIEAGARAG 237
Query: 227 IIEPDKKTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFP 275
+I PD+ T +Y++ ++A ++ +SD DAKY + D + P + +
Sbjct: 238 MIAPDETTFEYIRGRPLAPTGAEWDKAVEYWRSLRSDPDAKYDVDVFIDAADIAPTLTWG 297
Query: 276 HLPENTV--------------PISKAA---------------KMNIKIDQVFIGSCTNGR 306
P++ V PI + A +KID+VFIGSCTN R
Sbjct: 298 TSPQDVVAITGTTPDPSTVSDPIRRQAMERALDYIGLKPNTPMQEVKIDKVFIGSCTNSR 357
Query: 307 IEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCL 366
IEDLR AA I +G+ VA WV +V+P S V +A +EGL IF EAG C CL
Sbjct: 358 IEDLRAAAAIAKGRHVADWVYAMVVPGSGLVKKQAEQEGLDRIFKEAGFDWREAGCSMCL 417
Query: 367 GGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLG 413
G + L GER ST+NRNF GR G +L +P +AAA+AV G
Sbjct: 418 GMNPDQLKPGERCASTSNRNFEGRQG-AGGRTHLMSPAMAAAAAVTG 463
>sp|O33123|LEU2_MYCLE 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|11280213|pir||T45425 3-isopropylmalate dehydratase
[imported] - Mycobacterium leprae
>gi|2414558|emb|CAB16447.1| (Z99263) 3-isopropylmalate
dehydratase [Mycobacterium leprae]
Length = 476
Score = 245 bits (618), Expect = 9e-64
Identities = 165/472 (34%), Positives = 243/472 (50%), Gaps = 59/472 (12%)
Query: 4 TIAEKILADH-----SEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
T+AEK+ DH + + +++ L V ++VT P A R G R R
Sbjct: 9 TLAEKVWDDHVVVAGQGYHQDRGPDLIYIDLHLV--HEVTSPQAFDGLRLAG--RWVRRP 64
Query: 59 RIAIVL-DHFTPNKDIK-------SAEQCKSSREFAKEMGIKWFFEGG-SVGVEHCLLPE 109
+ + DH P DI S Q ++ R E G++ G G+ H + P+
Sbjct: 65 DLTVATEDHNVPTVDIDKPIADPVSRIQVETLRRNCAEFGVRLHPMGDIEQGIVHVVGPQ 124
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGEL 169
LGL PG I+ DSHT T+GA GA A G+G++++ +AT R +TM +G L
Sbjct: 125 LGLTQPGMTIVCGDSHTSTHGAFGAIAMGIGTSEIEHVLATQTLPLRPFKTMAVTVDGRL 184
Query: 170 QPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIE 229
VT KD+IL I IG G V+E+ GSVIE +S+E RMT+ NM+IEAGA+ G++
Sbjct: 185 PAGVTAKDIILALIAKIGTGGGQGYVLEYRGSVIESMSMEGRMTICNMSIEAGARAGMVA 244
Query: 230 PDKKTLDYVKER-----------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLP 278
PD+ T +++++R A ++ ++D+DA + + D T+ P V + P
Sbjct: 245 PDETTYEFLRDRPHAPTGKQWDAAVAYWQQLRTDDDAVFDTEVYLDATSLSPFVTWGTNP 304
Query: 279 ENTVPISKAAK-----------------------------MNIKIDQVFIGSCTNGRIED 309
VP++ + +I +D VF+GSCTNGRIED
Sbjct: 305 GQGVPLAASVPDPELMIDDVARQAAEKALAYMDLRPGTPMRDIAVDAVFVGSCTNGRIED 364
Query: 310 LRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGH 369
LR+ A++L G KVA VR++++P S V +A EGL EIF+ AGA P C CLG +
Sbjct: 365 LRLVADVLRGHKVADGVRMLIVPGSMRVRAQAEAEGLGEIFITAGAQWRQPGCSMCLGMN 424
Query: 370 MGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEEV 421
LA GER +T+NRNF GR G +L +P VAAA+AV G ++P ++
Sbjct: 425 PDQLAPGERCAATSNRNFEGRQG-KGGRTHLVSPAVAAATAVRGTFSAPADL 475
>sp|P56934|LEU2_BUCAI 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|5912538|emb|CAB56192.1| (AJ006878) isopropylmalate
dehydratase subunit [Buchnera aphidicola]
Length = 471
Score = 244 bits (616), Expect = 2e-63
Identities = 162/465 (34%), Positives = 250/465 (52%), Gaps = 58/465 (12%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ +KI H EE ++ L + ++VT P A R+ ++V ++
Sbjct: 1 MKKTLYDKIYDSHIVYEEKNNTSLLYIDLHLL--HEVTSPQAFDSLRDKN-RKVRQPKKT 57
Query: 61 AIVLDH--FTPNKDIKSA---------EQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPE 109
+DH T ++DI ++ E K+ EF + + + + G+ H + PE
Sbjct: 58 FATMDHNVSTTSQDINASGSMAKVQMQELIKNCSEFNISL---YDIKNPNQGIVHVISPE 114
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGEL 169
G+ LPG I+ DSHT T+GA GA + G+ ++++ +AT + + MK GE+
Sbjct: 115 KGMTLPGVTIVCGDSHTSTHGAFGALSFGIDTSEVEHVLATQTLKQQRFKNMKIEITGEI 174
Query: 170 QPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIE 229
Q +VT KDLIL IG +G +G ++EF G+VIE++S+E+RMT+ NMAIE GAK+G+I
Sbjct: 175 QKFVTAKDLILFIIGKLGSSGGAGYIIEFCGNVIEKMSMEERMTICNMAIEIGAKSGLIA 234
Query: 230 PDKKTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLP 278
PD+ T Y+K ++A +K KSD++A + KV+ ++++ P + + P
Sbjct: 235 PDEVTFSYLKNKMYAPRGVFWKKALNFWKNLKSDKNAFFDKVVNINISDLSPQITWGTNP 294
Query: 279 ENTVPISK-------------------AAKM----------NIKIDQVFIGSCTNGRIED 309
+ + I + A K NI +D+VFIGSCTNGRIED
Sbjct: 295 DQVISIDQKIPDFSSFDNLIKKDLAKSACKYMGLKIGTYLTNITVDEVFIGSCTNGRIED 354
Query: 310 LRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGH 369
LR A++IL+ +K+A V+ IV+P S +V +A EGL +IF+ AG P C CLG +
Sbjct: 355 LRAASKILKDKKIANNVKAIVVPGSSSVKREAENEGLDKIFINAGFEWRLPGCSMCLGMN 414
Query: 370 MGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGR 414
L GER ST+NRNF GR G +L +P +AAA+AV G+
Sbjct: 415 KDRLNDGERCASTSNRNFEGRQGR-GGRTHLVSPIMAAAAAVYGK 458
>gi|6320440 homoaconitase; Lys4p [Saccharomyces cerevisiae]
>gi|1346476|sp|P49367|LYS4_YEAST HOMOACONITASE PRECURSOR
(HOMOACONITATE HYDRATASE) >gi|2131287|pir||S61067
homoaconitate hydratase (EC 4.2.1.36) precursor - yeast
(Saccharomyces cerevisiae) >gi|1089840|emb|CAA63764.1|
(X93502) homoaconitase [Saccharomyces cerevisiae]
>gi|1197581|gb|AAA88902.1| (U46154) lysine biosynthesis
enzyme homoaconitase [Saccharomyces cerevisiae]
Length = 693
Score = 244 bits (616), Expect = 2e-63
Identities = 150/451 (33%), Positives = 238/451 (52%), Gaps = 33/451 (7%)
Query: 2 GMTIAEKILADHS----EREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDR 57
G + EKI+ ++ E + V G+ V K +D + P+A+K F LG ++ +
Sbjct: 20 GQNLTEKIVQSYAVNLPEGKVVHSGDYVSIKPAHCMSHDNSWPVALK-FMGLGATKIKNP 78
Query: 58 ERIAIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGD 117
+I LDH NK K+ + K+ FAK+ I + G +G H ++ E G P +
Sbjct: 79 SQIVTTLDHDIQNKSEKNLTKYKNIENFAKKHHIDHYPAGRGIG--HQIMIEEGYAFPLN 136
Query: 118 LIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKD 177
+ + +DSH+ TYG LG+ T + TD A ATG+ W+++P + +G+L V+GKD
Sbjct: 137 MTVASDSHSNTYGGLGSLGTPIVRTDAAAIWATGQTWWQIPPVAQVELKGQLPQGVSGKD 196
Query: 178 LILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDY 237
+I+ G + L +EF+G + L ++ R+T++NM E GA +G+ DK +D+
Sbjct: 197 IIVALCGLFNNDQVLNHAIEFTGDSLNALPIDHRLTIANMTTEWGALSGLFPVDKTLIDW 256
Query: 238 VKERAKR-------------------KFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLP 278
K R ++ K K+ K+D+DA Y K + D+ V+ P+
Sbjct: 257 YKNRLQKLGTNNHPRINPKTIRALEEKAKIPKADKDAHYAKKLIIDLATLTHYVSGPNSV 316
Query: 279 ENTVPISKAAKMNIKIDQVFIGSCTNGRIEDLRMAAEIL-------EGQKVAKWVRLIVI 331
+ + + ++ +IKI++ ++ SCTN R+ DL+ AA+++ + KVA V V
Sbjct: 317 KVSNTVQDLSQQDIKINKAYLVSCTNSRLSDLQSAADVVCPTGDLNKVNKVAPGVEFYVA 376
Query: 332 PCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRM 391
S + A K G E L+AG + P CGPC+G G+L GE +S TNRNF GRM
Sbjct: 377 AASSEIEADARKSGAWEKLLKAGCIPLPSGCGPCIGLGAGLLEPGEVGISATNRNFKGRM 436
Query: 392 GHPKSEVYLANPYVAAASAVLGRIASPEEVV 422
G + YLA+P V AASAVLG+I+SP EV+
Sbjct: 437 GSKDALAYLASPAVVAASAVLGKISSPAEVL 467
>emb|CAA72703.1| (Y11966) 3-isopropylmalate dehydratase subunit [Buchnera
aphidicola]
Length = 466
Score = 242 bits (610), Expect = 8e-63
Identities = 164/462 (35%), Positives = 239/462 (51%), Gaps = 54/462 (11%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRE-R 59
MG T+ EK+ H E+ I+ L + ++VT P A + + KR+ +
Sbjct: 1 MGQTLYEKLYNSHIIYEDKNTLPIIYIDLHLL--HEVTSPQAFESLKNK--KRIVHAPIK 56
Query: 60 IAIVLDHFTPNKDIKSAEQCKSSR-------EFAKEMGIKWF-FEGGSVGVEHCLLPELG 111
+DH K K + K+++ K+ IK + + G+ H + PE G
Sbjct: 57 TFATMDHNVSTKTNKISASGKAAQIQMQQLINNCKDFNIKLYDLNHINQGIVHVMGPEQG 116
Query: 112 LVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQP 171
L LPG I+ DSHT T+GA G A G+G++++ +AT +TMK G L+P
Sbjct: 117 LTLPGMTIVCGDSHTSTHGAFGTLAFGIGTSEVEHVLATQTLKQARSKTMKIGINGILKP 176
Query: 172 YVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPD 231
Y+T KD+IL+ I +G V+EFSG IE L++E RMT+ NM IE GAK+GII PD
Sbjct: 177 YITAKDVILYIIKKVGTAFGTGYVVEFSGETIENLTMEGRMTICNMVIEMGAKSGIIAPD 236
Query: 232 KKTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
T Y+K + A +K K+D DAK+ K++++DV+ EP + + P
Sbjct: 237 ATTYKYLKNLPYAPKKEKWKNALEYWKNLKTDNDAKFDKIVKFDVSQIEPQITWGTNPSQ 296
Query: 281 TVPIS-------------------KAAK----------MNIKIDQVFIGSCTNGRIEDLR 311
T+ I+ KA K +N+ +D+VFIGSCTN RIEDLR
Sbjct: 297 TINITESNPDPKKINNIIDKQATEKALKYMNLKPNQKMINLVVDKVFIGSCTNSRIEDLR 356
Query: 312 MAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMG 371
+A++I++ +KVAK + IV+P S V +A KEGL +IF+ AG P C CL +
Sbjct: 357 IASKIIKNKKVAKTTKAIVVPGSKLVKIQAEKEGLDKIFINAGFEWRLPGCSMCLAMNDD 416
Query: 372 VLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLG 413
L GER ST+NRNF R G +L +P AAA+A+ G
Sbjct: 417 KLNRGERCASTSNRNFEDRQGR-GGRTHLVSPITAAAAAIFG 457
>sp|Q44427|LEU2_ACTTI 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|2127501|pir||I39699 3-isopropylmalate dehydratase
(EC 4.2.1.33) large chain - Actinoplanes teichomyceticus
>gi|1008942|emb|CAA59140.1| (X84647) large subunit of
isopropilmalate isomerase [Actinoplanes teichomyceticus]
Length = 485
Score = 242 bits (610), Expect = 8e-63
Identities = 167/476 (35%), Positives = 245/476 (51%), Gaps = 61/476 (12%)
Query: 4 TIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIAIV 63
T+AEK+ DH R + GE + +D ++VT P A R G +RV +
Sbjct: 13 TLAEKVWDDHVVRTAAE-GEPDLLFIDLHLLHEVTSPQAFDGLRMAG-RRVRRTDLTLAT 70
Query: 64 LDHFTPN-----------------KDIKSAEQCKSSREFAKEMGIKWFFEGG-SVGVEHC 105
DH TP D S Q ++ R+ E G++ G + G+ H
Sbjct: 71 EDHNTPTGYADPSFNTRRGELLTIADTVSRTQIETLRKNCAEFGVEIRPLGDVNQGIVHV 130
Query: 106 LLPELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIY 165
+ P+LGL PG I+ DSHT T+GA GA A G+G++++ +AT P+TM
Sbjct: 131 IGPQLGLTQPGMTIVCGDSHTATHGASGALAFGIGTSEVEHVLATQTLPQSKPKTMAVTV 190
Query: 166 EGELQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKT 225
GEL+P V+ KDLIL I G G ++E+ G I +LS+E RMT+ NM+IE GAK
Sbjct: 191 VGELRPGVSAKDLILTLITQTGTGGGNGHIVEYRGEAIRKLSMEGRMTICNMSIEWGAKA 250
Query: 226 GIIEPDKKTLDYVKER-----------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAF 274
G+I PD+ T DY+ R A +K +DE A+Y I D + P + +
Sbjct: 251 GMIAPDETTFDYLSGRKHARRGADWDAAVAYWKTLATDEGAEYDTEIILDASKISPFITW 310
Query: 275 PHLP------ENTVP--------ISKAAK---------------MNIKIDQVFIGSCTNG 305
P + VP + + A ++ +D VF+GSCTNG
Sbjct: 311 GTNPGQGAALDGVVPDPQDFLDEVERGAAERALAYMGLTPGTPFRDVPVDVVFVGSCTNG 370
Query: 306 RIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPC 365
R+EDLR AA+++ G+KVA VR++++P S V +A EGL +IF++AGA C C
Sbjct: 371 RLEDLRAAADVIRGRKVADGVRMMIVPGSYQVREQAEAEGLDKIFIDAGAEWRFAGCSMC 430
Query: 366 LGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEEV 421
LG + L+ G+RA ST+NRNF GR G +L +P VAAA+AV+G++A+P ++
Sbjct: 431 LGMNPDTLSPGQRAASTSNRNFEGRQG-KGGRTHLVSPQVAAATAVVGKLAAPADL 485
>sp|O53237|LEU2_MYCTU 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7450356|pir||G70853 probable leuC protein -
Mycobacterium tuberculosis (strain H37RV)
>gi|2791585|emb|CAA16073.1| (AL021287) leuC
[Mycobacterium tuberculosis]
Length = 473
Score = 241 bits (609), Expect = 1e-62
Identities = 170/468 (36%), Positives = 238/468 (50%), Gaps = 55/468 (11%)
Query: 4 TIAEKILADHSEREEVKPGEIV--MAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIA 61
T+AEKI DH V G + +D ++VT P A R G +RV E
Sbjct: 10 TLAEKIWDDHIV---VSGGGCAPDLIYIDLHLVHEVTSPQAFDGLRLAG-RRVRRPELTL 65
Query: 62 IVLDHFTPNKDIK-------SAEQCKSSREFAKEMGIKWFFEGG-SVGVEHCLLPELGLV 113
DH P DI S Q ++ R E GI+ G G+ H + P+LGL
Sbjct: 66 ATEDHNVPTVDIDQPIADPVSRTQVETLRRNCAEFGIRLHSMGDIEQGIVHVVGPQLGLT 125
Query: 114 LPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYV 173
PG I+ DSHT T+GA GA A G+G++++ +AT R +TM +G L V
Sbjct: 126 QPGMTIVCGDSHTSTHGAFGALAMGIGTSEVEHVLATQTLPLRPFKTMAVNVDGRLPDGV 185
Query: 174 TGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKK 233
+ KD+IL I IG G V+E+ GS IE LS+E RMT+ NM+IEAGA+ G++ PD+
Sbjct: 186 SAKDIILALIAKIGTGGGQGHVIEYRGSAIESLSMEGRMTICNMSIEAGARAGMVAPDET 245
Query: 234 TLDYVKER-----------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTV 282
T +++ R A ++ ++D A + + D + P V + P V
Sbjct: 246 TYAFLRGRPHAPTGAQWDTALVYWQRLRTDVGAVFDTEVYLDAASLSPFVTWGTNPGQGV 305
Query: 283 PISKA-----------------------------AKMNIKIDQVFIGSCTNGRIEDLRMA 313
P++ A A +I +D VF+GSCTNGRIEDLR+
Sbjct: 306 PLAAAVPDPQLMTDDAERQAAEKALAYMDLRPGTAMRDIAVDAVFVGSCTNGRIEDLRVV 365
Query: 314 AEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVL 373
AE+L G+KVA VR++++P S V +A EGL EIF +AGA C CLG + L
Sbjct: 366 AEVLRGRKVADGVRMLIVPGSMRVRAQAEAEGLGEIFTDAGAQWRQAGCSMCLGMNPDQL 425
Query: 374 ASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEEV 421
ASGER +T+NRNF GR G +L +P VAAA+AV G ++SP ++
Sbjct: 426 ASGERCAATSNRNFEGRQG-AGGRTHLVSPAVAAATAVRGTLSSPADL 472
>sp|P49601|LEU2_USTMA 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMALATE ISOMERASE)
(ALPHA-IPM ISOMERASE) (IPMI) >gi|311008|gb|AAA34226.1|
(L20832) LEU1 [Ustilago maydis]
Length = 773
Score = 241 bits (609), Expect = 1e-62
Identities = 170/474 (35%), Positives = 234/474 (48%), Gaps = 59/474 (12%)
Query: 4 TIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIAIV 63
T+ EKI H EE ++ V ++VT P A + R ++V +
Sbjct: 5 TLYEKIFDSHLVHEEADGTCLIYIDRHLV--HEVTSPQAFEGLRNAN-RKVRRTDCTLAT 61
Query: 64 LDHFTPN-------------KDIKSAEQCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPE 109
+DH P + + S QC + E K G+ +F G+ H + PE
Sbjct: 62 VDHNIPTASRKSYRDTKSFVEQVDSRTQCMTLEENVKAFGLTFFGLSDNRQGIVHIIGPE 121
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGEL 169
G LPG I+ DSHT T+GA GA A G+G++++ +AT + + M +GEL
Sbjct: 122 QGFTLPGATIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLLQKRAKNMLIQVDGEL 181
Query: 170 QPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIE 229
VT KD+ILH IG IG G V+E++GS I LS+E RM++ NM+IEAGA+ G+I
Sbjct: 182 SQGVTSKDIILHIIGLIGTAGGTGHVIEYAGSTIRSLSMEARMSICNMSIEAGARAGLIA 241
Query: 230 PDKKTLDYVKER------------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHL 277
PD+ T +Y+K R A +K SDE A+Y VI+ D + P V +
Sbjct: 242 PDEITYEYIKGRPLAPKQGEAWDQALAYWKTLPSDEGAQYDTVIKIDAKDIPPTVTWGTS 301
Query: 278 PEN------TVPISKAAKMN-----------------------IKIDQVFIGSCTNGRIE 308
P++ TVP K A IKID+VFIGSCTN RIE
Sbjct: 302 PQDVVAITGTVPDPKNASNEAEAKAWTRALEYMGLEAGTPMEKIKIDKVFIGSCTNARIE 361
Query: 309 DLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGG 368
DLR AA +L G+KVA + +++P S V +A EGL +IF AG C CLG
Sbjct: 362 DLRAAARVLHGRKVADGLYCMLVPGSGLVKKQAEAEGLDKIFQAAGFDWREAGCSMCLGM 421
Query: 369 HMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEEVV 422
+ LA GER ST+NRNF GR G +L +P +AAA AV G + +VV
Sbjct: 422 NPDQLAPGERCASTSNRNFEGRQG-AGGRTHLMSPAMAAACAVTGYLTDVRKVV 474
>dbj|BAB06775.1| (AP001517) 3-isopropylmalate dehydratase large subunit [Bacillus
halodurans]
Length = 472
Score = 241 bits (609), Expect = 1e-62
Identities = 167/458 (36%), Positives = 236/458 (51%), Gaps = 53/458 (11%)
Query: 4 TIAEKILADHSE-REEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIAI 62
TI EKI H+ EE KP + +D ++VT P A + R G + V +
Sbjct: 5 TIIEKIWDAHTVIGEEGKPS---LLYIDLHMVHEVTSPQAFEGLRLAG-RPVRRPDLTFA 60
Query: 63 VLDHFTPN------KDIKSAEQCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPELGLVLP 115
+DH P +D + +Q ++ KE GI+ + + G+ H + PELGL P
Sbjct: 61 TMDHNVPTVDRFNIQDQIARKQIETLEANCKEFGIEIAGLDSPNNGIVHVIGPELGLTQP 120
Query: 116 GDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTG 175
G I+ DSHT T+GA GA A G+G++++ +AT W P+TM+ GEL P V+
Sbjct: 121 GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLWQSKPKTMEVRVTGELAPSVSA 180
Query: 176 KDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTL 235
KD+IL I GV+ V+EF+G I LS+E+RMT+ NM+IEAGAK G+I PD T
Sbjct: 181 KDIILAVIAKYGVDFGTGHVIEFTGEAIRSLSMEERMTICNMSIEAGAKAGLISPDSVTF 240
Query: 236 DYVKER-----------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLP------ 278
+Y++ R A ++++ +D A Y +V+ + + EP+V + P
Sbjct: 241 EYLRGRPNAPKGEAFDVAIQQWEALATDAGAVYDRVLTMNASEIEPMVTWGTNPAQGTGV 300
Query: 279 ENTVPISKAAK-----------------------MNIKIDQVFIGSCTNGRIEDLRMAAE 315
VP AK I I VFIGSCTN R+ DLR AAE
Sbjct: 301 SQVVPSPDDAKDENERRAIKQSLAYMGLEPGTPITEIAIQHVFIGSCTNSRLSDLRTAAE 360
Query: 316 ILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLAS 375
+++G+KVA VR +V+P S V A KEGL EIF EAG C CLG + +
Sbjct: 361 LIKGRKVADGVRALVVPGSQQVKRAAEKEGLDEIFKEAGFEWRDSGCSMCLGMNPDTVPE 420
Query: 376 GERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLG 413
GER ST+NRNF GR G + +L +P +AAA+A+ G
Sbjct: 421 GERCASTSNRNFEGRQG-KGARTHLVSPQMAAAAAIAG 457
>sp|P80858|LEU2_BACSU 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
(SUPEROXIDE-INDUCIBLE PROTEIN 10) (SOI10)
>gi|7450355|pir||B69650 3-isopropylmalate dehydratase
(EC 4.2.1.33) large chain - Bacillus subtilis
>gi|1770071|emb|CAA99533.1| (Z75208) 3-isopropylmalate
dehydratase, large subunit [Bacillus subtilis]
>gi|2635291|emb|CAB14786.1| (Z99118) 3-isopropylmalate
dehydratase (large subunit) [Bacillus subtilis]
Length = 472
Score = 240 bits (607), Expect = 2e-62
Identities = 160/461 (34%), Positives = 237/461 (50%), Gaps = 53/461 (11%)
Query: 1 MGMTIAEKILADHSERE-EVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRER 59
M TI EKI H + E KP + +D ++VT P A + R+ G ++V +
Sbjct: 2 MPRTIIEKIWDQHIVKHGEGKPD---LLYIDLHLIHEVTSPQAFEGLRQKG-RKVRRPQN 57
Query: 60 IAIVLDHFTPN------KDIKSAEQCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPELGL 112
+DH P KD + Q + +E G++ G+ H + PELGL
Sbjct: 58 TFATMDHNIPTVNRFEIKDEVAKRQVTALERNCEEFGVRLADLHSVDQGIVHVVGPELGL 117
Query: 113 VLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPY 172
LPG I+ DSHT T+GA GA A G+G++++ ++T W + P+T++ +G LQ
Sbjct: 118 TLPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQQRPKTLEVRVDGTLQKG 177
Query: 173 VTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDK 232
VT KD+IL IG GV V+E++G V +++++RMT+ NM+IEAGA+ G+I PD+
Sbjct: 178 VTAKDVILAVIGKYGVKFGTGYVIEYTGEVFRNMTMDERMTVCNMSIEAGARAGLIAPDE 237
Query: 233 KTLDYVKER-----------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENT 281
T +Y K R A ++K ++D A Y K I D P+V + P
Sbjct: 238 VTFEYCKNRKYTPKGEEFDKAVEEWKALRTDPGAVYDKSIVLDGNKISPMVTWGINPGMV 297
Query: 282 VPISKAAKM-----------------------------NIKIDQVFIGSCTNGRIEDLRM 312
+P+ +IK++ VFIGSCTN R+ DLR
Sbjct: 298 LPVDSEVPAPESFSAEDDKKEAIRAYEYMGLTPHQKIEDIKVEHVFIGSCTNSRMTDLRQ 357
Query: 313 AAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGV 372
AA++++G+KVA VR IV+P S +V +A KEGL +IFLEAG C CL + V
Sbjct: 358 AADMIKGKKVADSVRAIVVPGSQSVKLQAEKEGLDQIFLEAGFEWRESGCSMCLSMNNDV 417
Query: 373 LASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLG 413
+ GER ST+NRNF GR G + +L +P +AA +A+ G
Sbjct: 418 VPEGERCASTSNRNFEGRQG-KGARTHLVSPAMAAMAAIHG 457
>sp|O14289|LEU2_SCHPO 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMALATE ISOMERASE)
(ALPHA-IPM ISOMERASE) (IPMI) >gi|7489951|pir||T39210
3-isopropylmalate dehydratase - fission yeast
(Schizosaccharomyces pombe) >gi|2414659|emb|CAB16402.1|
(Z99262) 3-isopropylmalate dehydratase
[Schizosaccharomyces pombe]
Length = 758
Score = 236 bits (597), Expect = 3e-61
Identities = 162/441 (36%), Positives = 230/441 (51%), Gaps = 57/441 (12%)
Query: 35 NDVTMPLAIKKFRELGVKRVFDRERIAIVLDH---FTPNKDIK----------SAEQCKS 81
++VT P A + R G ++V E +DH P KD+K S Q +
Sbjct: 39 HEVTSPQAFEGLRTAG-RKVRHPELALATVDHNIPTDPRKDMKDIASFIHQPDSRTQVLA 97
Query: 82 SREFAKEMGIKWF-FEGGSVGVEHCLLPELGLVLPGDLIIGADSHTCTYGALGAFATGVG 140
KE G+ ++ G+ H + PE G LPG ++ DSHT T+GA GA A G+G
Sbjct: 98 LENNIKEFGLTYYGMNDRRQGIVHVIGPEQGFTLPGTTLVCGDSHTSTHGAFGALAFGIG 157
Query: 141 STDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLILHTIGDIGVNGALYKVMEFSG 200
++++ +AT R + M+ G+L + KDLILH IG IG G V+EF G
Sbjct: 158 TSEVEHVLATQTILQRKSKNMRIRVNGKLPEGIASKDLILHIIGVIGTAGGTGSVIEFCG 217
Query: 201 SVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVK-----------ERAKRKFKVY 249
IE LS+E RM+M NM+IEAGA+ G+I PD T +YVK E+A +K
Sbjct: 218 EAIEGLSMEARMSMCNMSIEAGARAGMIAPDATTFEYVKNRPLAPKGDDWEQAVAYWKTL 277
Query: 250 KSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPIS-------------KAAK------- 289
+SDE+AKY +E + + P V + P++ +PI+ +AA
Sbjct: 278 RSDENAKYDIEVEINAADVLPTVTWGTSPQDVIPINGNIPDPAHVKDNVRAASIQRSLEY 337
Query: 290 MNIK---------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR-LIVIPCSPTVYW 339
M +K ID+VFIGSCTN RIEDLR+AA +++G+KVA V+ +++P S V
Sbjct: 338 MGLKPNTSIVSYPIDKVFIGSCTNSRIEDLRLAAAVVKGRKVAANVKDAMIVPGSGLVKK 397
Query: 340 KALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVY 399
A EGL +IF+EAG C CLG + L ER ST+NRNF GR G K +
Sbjct: 398 MAEAEGLDQIFIEAGFDWREAGCSMCLGMNPDQLKPYERCASTSNRNFEGRQG-AKGRTH 456
Query: 400 LANPYVAAASAVLGRIASPEE 420
L +P +AAA+A+ G + + E
Sbjct: 457 LVSPAMAAAAAIKGHLCNVRE 477
>pir||S26864 3-isopropylmalate dehydratase (EC 4.2.1.33) - Phycomyces
blakesleeanus >gi|3113|emb|CAA37257.1| (X53090)
alpha-isopropylmalate isomerase (AA 1-689) [Phycomyces
blakesleeanus]
Length = 689
Score = 236 bits (596), Expect = 3e-61
Identities = 159/467 (34%), Positives = 234/467 (50%), Gaps = 70/467 (14%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ +K+ DH +D ++VT P A + R +RV +
Sbjct: 1 MSRTLYDKVWDDHV--------------IDRHLVHEVTSPQAFEGLRNAS-RRVRRPDCT 45
Query: 61 AIVLDHFTPNKDIK-------------SAEQCKSSREFAKEMGIKWF-FEGGSVGVEHCL 106
+DH P K S QC++ KE + +F E G+ H +
Sbjct: 46 LATVDHNIPTTTRKKFKSITTFIDEADSRTQCETLETNVKEFELTYFGMEDSRQGIVHVI 105
Query: 107 LPELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYE 166
PE G LPG ++ DSHT T+GA GA A G+G++++ +AT + + M+ E
Sbjct: 106 GPEQGFTLPGTTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLLQKKSKNMRVCVE 165
Query: 167 GELQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTG 226
GEL P VT KD+ LH IG IG G V+EF GS I LS+E RM++ NM+IE GA+ G
Sbjct: 166 GELTPGVTSKDVALHVIGLIGTAGGTRCVIEFCGSAIASLSMEARMSICNMSIEGGARAG 225
Query: 227 IIEPDKKTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFP 275
+I PD+ T +Y++ ++A + +K KSD +AKY ++ ++ P + +
Sbjct: 226 MIAPDEITFEYLRGRPLAPEGAEWDKAVQYWKSLKSDPNAKYDIDVKIAASDIAPTITWG 285
Query: 276 HLPENTVPIS--------------KAAK---------------MNIKIDQVFIGSCTNGR 306
P++ PI+ KAA +KID+ FIGSCTN R
Sbjct: 286 TSPQDVAPITANVPDPSSVSDPARKAAMERALEYIGLVPNTPLEEVKIDKAFIGSCTNSR 345
Query: 307 IEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCL 366
IEDLR AA I++G+ +A WV +V+P S V +A +EGL ++F +AG C CL
Sbjct: 346 IEDLRSAASIVKGKHIADWVYAMVVPGSGLVKRQAEREGLDKVFTDAGFDWREAGCSMCL 405
Query: 367 GGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLG 413
G + L+ GER ST+NRNF GR G +L +P +AAA+A+ G
Sbjct: 406 GMNPDQLSPGERCASTSNRNFEGRQG-AGGRTHLMSPAMAAAAAIRG 451
>sp|P18250|LEU2_PHYBL 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMALATE ISOMERASE)
(ALPHA-IPM ISOMERASE) (IPMI)
Length = 744
Score = 236 bits (596), Expect = 3e-61
Identities = 159/467 (34%), Positives = 234/467 (50%), Gaps = 70/467 (14%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ +K+ DH +D ++VT P A + R +RV +
Sbjct: 1 MSRTLYDKVWDDHV--------------IDRHLVHEVTSPQAFEGLRNAS-RRVRRPDCT 45
Query: 61 AIVLDHFTPNKDIK-------------SAEQCKSSREFAKEMGIKWF-FEGGSVGVEHCL 106
+DH P K S QC++ KE + +F E G+ H +
Sbjct: 46 LATVDHNIPTTTRKKFKSITTFIDEADSRTQCETLETNVKEFELTYFGMEDSRQGIVHVI 105
Query: 107 LPELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYE 166
PE G LPG ++ DSHT T+GA GA A G+G++++ +AT + + M+ E
Sbjct: 106 GPEQGFTLPGTTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLLQKKSKNMRVCVE 165
Query: 167 GELQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTG 226
GEL P VT KD+ LH IG IG G V+EF GS I LS+E RM++ NM+IE GA+ G
Sbjct: 166 GELTPGVTSKDVALHVIGLIGTAGGTRCVIEFCGSAIASLSMEARMSICNMSIEGGARAG 225
Query: 227 IIEPDKKTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFP 275
+I PD+ T +Y++ ++A + +K KSD +AKY ++ ++ P + +
Sbjct: 226 MIAPDEITFEYLRGRPLAPEGAEWDKAVQYWKSLKSDPNAKYDIDVKIAASDIAPTITWG 285
Query: 276 HLPENTVPIS--------------KAAK---------------MNIKIDQVFIGSCTNGR 306
P++ PI+ KAA +KID+ FIGSCTN R
Sbjct: 286 TSPQDVAPITANVPDPSSVSDPARKAAMERALEYIGLVPNTPLEEVKIDKAFIGSCTNSR 345
Query: 307 IEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCL 366
IEDLR AA I++G+ +A WV +V+P S V +A +EGL ++F +AG C CL
Sbjct: 346 IEDLRSAASIVKGKHIADWVYAMVVPGSGLVKRQAEREGLDKVFTDAGFDWREAGCSMCL 405
Query: 367 GGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLG 413
G + L+ GER ST+NRNF GR G +L +P +AAA+A+ G
Sbjct: 406 GMNPDQLSPGERCASTSNRNFEGRQG-AGGRTHLMSPAMAAAAAIRG 451
>emb|CAB99455.1| (AJ278693) 3-isopropylmalate dehydratase [Yarrowia lipolytica]
Length = 711
Score = 236 bits (596), Expect = 3e-61
Identities = 160/470 (34%), Positives = 236/470 (50%), Gaps = 60/470 (12%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
MG T+ +K+ ADH E+ + +D ++VT P A + + G ++V ++
Sbjct: 1 MGQTLYDKVFADHVVHED---NGTYLLFIDRHLVHEVTSPQAFEGLKNAG-RQVRRKDCT 56
Query: 61 AIVLDHFTPN---KDIKSAE----------QCKSSREFAKEMGIKWF-FEGGSVGVEHCL 106
+DH P KD KS QC + + K+ G+++F + G+ H +
Sbjct: 57 LATVDHNIPTTTRKDFKSIGTFIKEEDSRLQCTTLEQNVKDFGVRYFGMDDKRQGIVHVV 116
Query: 107 LPELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYE 166
PE G LPG ++ DSHT T+GA GA A G+G++++ +AT + M+
Sbjct: 117 GPEQGFTLPGTTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTIIQAKSKNMRINVS 176
Query: 167 GELQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTG 226
G+LQP +T KDLILH IG IG G V+EF G I +LS+E RM+M NMAIE GA+ G
Sbjct: 177 GKLQPGITSKDLILHIIGVIGTAGGTGTVIEFCGEAIRDLSMEARMSMCNMAIEGGARAG 236
Query: 227 IIEPDKKTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFP 275
+I PD+ T Y++ E+A +K SD DA++ + + P V +
Sbjct: 237 MIAPDETTFRYIRGRPLAPEGAEWEKAVTYWKTLHSDSDAEFDIDVNIKGEDIIPTVTWG 296
Query: 276 HLPENTVPISKAAK-----------------------------MNIKIDQVFIGSCTNGR 306
P++ +PI+ + I +D+VFIGSCTN R
Sbjct: 297 TSPQDALPITGSVPDPSKESDPIKRAGIERALEYMGLEANTPLNEIAVDKVFIGSCTNSR 356
Query: 307 IEDLRMAAEILEGQKVA-KWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPC 365
IEDLR AA I+ G+K A VR +V+P S V KA EGL ++F AG C C
Sbjct: 357 IEDLRAAAAIVRGKKKADSVVRAMVVPGSGLVKRKAEAEGLDKVFEAAGFEWREAGCSMC 416
Query: 366 LGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRI 415
LG + +L ER ST+NRNF GR G S +L +P +AA + + G++
Sbjct: 417 LGMNPDILNPRERCASTSNRNFEGRQG-ALSRTHLVSPAMAALAGITGKL 465
>gi|10954460 3-isopropylmalate isomerase subunit [Buchnera aphidicola]
>gi|3282742|gb|AAD12602.1| (AF041837) 3-isopropylmalate
isomerase subunit [Buchnera aphidicola]
Length = 466
Score = 236 bits (595), Expect = 5e-61
Identities = 160/469 (34%), Positives = 251/469 (53%), Gaps = 52/469 (11%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ EKI H E I+ L + ++VT P A + R + + V ++
Sbjct: 1 MNKTLYEKIYDSHVVHSEKNGLSILYVDLHLL--HEVTSPQAFESLR-IKDRTVRQPKKT 57
Query: 61 AIVLDH--FTPNKDIKSAE-----QCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPELGL 112
+DH T +KDI ++ Q + + KE I + + G+ H + PE G+
Sbjct: 58 FATMDHNVSTESKDINASGSMAKIQMQQLIKNCKEFHISLYDLNHPNQGIVHVISPEQGM 117
Query: 113 VLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPY 172
LPG +I+ DSHT T+GA GA + G+G++++ +AT + + MK G++ +
Sbjct: 118 TLPGMVIVCGDSHTSTHGAFGALSFGIGTSEVEHVLATQTLKQQRFKNMKIEVIGKIGNF 177
Query: 173 VTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDK 232
+T KD+IL+ IG IG + ++EF G+VI+++S+E+RMT+ NMAIE GAK+G+I PD+
Sbjct: 178 ITAKDVILYIIGKIGSSAGTGYIIEFCGNVIKKMSMEERMTVCNMAIELGAKSGLIAPDE 237
Query: 233 KTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENT 281
T Y+K ++A +K K+D +A + KV D++N P V + P+
Sbjct: 238 TTYLYLKNKTYSPHGRNWQKAIEYWKTLKTDHNAIFDKVFTIDISNILPQVTWGTNPDQV 297
Query: 282 VPISK-------------------AAKM----------NIKIDQVFIGSCTNGRIEDLRM 312
+ I++ A K +I ID+VFIGSCTN RIEDLR
Sbjct: 298 IGINEKIPDFTSFQNIVKKDLAKSACKYMDLKPGTYLTDITIDKVFIGSCTNSRIEDLRS 357
Query: 313 AAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGV 372
A++IL+ K++K V+ IV+P S V +A EGL +IF+EAG P C CLG +
Sbjct: 358 ASKILKHNKISKNVKAIVVPGSGLVKRQAESEGLDKIFIEAGFEWRLPGCSMCLGMNNDR 417
Query: 373 LASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEEV 421
L+ ER ST+NRNF GR G +L +P +AA +A+ G+ ++P ++
Sbjct: 418 LSENERCASTSNRNFEGRQGR-NGRTHLVSPIMAALAALYGKFSNPNKL 465
>gi|6321429 isopropylmalate isomerase; Leu1p [Saccharomyces cerevisiae]
>gi|1708801|sp|P07264|LEU2_YEAST 3-ISOPROPYLMALATE
DEHYDRATASE (ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM
ISOMERASE) (IPMI) >gi|2131159|pir||S64011
3-isopropylmalate dehydratase (EC 4.2.1.33) - yeast
(Saccharomyces cerevisiae) >gi|1322463|emb|CAA96709.1|
(Z72531) ORF YGL009c [Saccharomyces cerevisiae]
Length = 779
Score = 235 bits (592), Expect = 1e-60
Identities = 155/441 (35%), Positives = 227/441 (51%), Gaps = 57/441 (12%)
Query: 35 NDVTMPLAIKKFRELGVKRVFDRERIAIVLDHFTPN---KDIKSAE----------QCKS 81
++VT P A + G ++V + +DH P K+ KS + Q K+
Sbjct: 40 HEVTSPQAFEGLENAG-RKVRRVDCTLATVDHNIPTESRKNFKSLDTFIKQTDSRLQVKT 98
Query: 82 SREFAKEMGIKWF-FEGGSVGVEHCLLPELGLVLPGDLIIGADSHTCTYGALGAFATGVG 140
K+ G+ +F G+ H + PE G LPG ++ DSHT T+GA G+ A G+G
Sbjct: 99 LENNVKQFGVPYFGMSDARQGIVHTIGPEEGFTLPGTTVVCGDSHTSTHGAFGSLAFGIG 158
Query: 141 STDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLILHTIGDIGVNGALYKVMEFSG 200
++++ +AT + M+ G+L P +T KDLIL+ IG IG G V+EF+G
Sbjct: 159 TSEVEHVLATQTIIQAKSKNMRITVNGKLSPGITSKDLILYIIGLIGTAGGTGCVIEFAG 218
Query: 201 SVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVK-----------ERAKRKFKVY 249
IE LS+E RM+M NMAIEAGA+ G+I+PD+ T Y K E+A +K
Sbjct: 219 EAIEALSMEARMSMCNMAIEAGARAGMIKPDETTFQYTKGRPLAPKGAEWEKAVAYWKTL 278
Query: 250 KSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAK-------------------- 289
K+DE AK+ I + + P + + P++ +PI+ +
Sbjct: 279 KTDEGAKFDHEINIEAVDVIPTITWGTSPQDALPITGSVPDPKNVTDPIKKSGMERALAY 338
Query: 290 ---------MNIKIDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVRL-IVIPCSPTVYW 339
+IK+D+VFIGSCTNGRIEDLR AA ++ GQK+A ++L +V+P S V
Sbjct: 339 MGLEPNTPLKSIKVDKVFIGSCTNGRIEDLRSAAAVVRGQKLASNIKLAMVVPGSGLVKK 398
Query: 340 KALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVY 399
+A EGL +IF EAG C CLG + +L + ER ST+NRNF GR G S +
Sbjct: 399 QAEAEGLDKIFQEAGFEWREAGCSICLGMNPDILDAYERCASTSNRNFEGRQG-ALSRTH 457
Query: 400 LANPYVAAASAVLGRIASPEE 420
L +P +AAA+ + G E
Sbjct: 458 LMSPAMAAAAGIAGHFVDIRE 478
>sp|Q00464|LEU2_CANMA 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMALATE ISOMERASE)
(ALPHA-IPM ISOMERASE) (IPMI) >gi|1399939|gb|AAB03335.1|
(U60167) alpha isopropylmalate isomerase [Candida
maltosa]
Length = 770
Score = 234 bits (590), Expect = 2e-60
Identities = 156/472 (33%), Positives = 240/472 (50%), Gaps = 59/472 (12%)
Query: 4 TIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIAIV 63
T+ +K+ DH E+ ++ V ++VT P A + + G + V +
Sbjct: 5 TLYDKVFEDHIVYEDESGSNLLYIDRHLV--HEVTSPQAFEGLKNAG-RTVRRTDCTLAT 61
Query: 64 LDHFTPN-------------KDIKSAEQCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPE 109
+DH P S Q ++ + K+ + +F G+ H + PE
Sbjct: 62 VDHNIPTISRVNFKTLLTFIDQDDSRLQVQTLEQNVKDFDVTYFGMTDDRQGIVHVVGPE 121
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGEL 169
G LPG ++ DSHT T+GA GA A G+G++++ +AT P+ M+ +GEL
Sbjct: 122 QGFTLPGTTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTTIQANPKNMRITIDGEL 181
Query: 170 QPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIE 229
+T KDL+LH IG IG G V+EF+G IE+LS+E RM++ NMAIEAGA+ G+I+
Sbjct: 182 SEGITSKDLVLHVIGVIGTAGGTGCVIEFAGKAIEDLSMEARMSICNMAIEAGARAGMIK 241
Query: 230 PDKKTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLP 278
PD+KT +Y+K E+A + +K +D+ AK + I+ ++ P + + + P
Sbjct: 242 PDEKTFEYIKGRPLAPKGDEWEKALKYWKTLHTDDGAKLHYDIKIAASDIVPTITWGNSP 301
Query: 279 ENTVPIS-------------------KAAKMN----------IKIDQVFIGSCTNGRIED 309
++ +PI+ +A K IK+ + FIGSCTN RIED
Sbjct: 302 QDALPITASVPDPANVSDPIKKSGMERALKYQGLTPNTPFVVIKMHKAFIGSCTNSRIED 361
Query: 310 LRMAAEILEGQKVAKWVRLI-VIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGG 368
LR AA++ +G K A V+L+ V+P S + +A KEGL +IF AG C CLG
Sbjct: 362 LRAAAKVAKGHKKADNVKLVLVVPGSGLIKKQAEKEGLDKIFESAGFTWREAGCSMCLGM 421
Query: 369 HMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEE 420
+ +L ER ST+NRNF GR G +S +L +P +AAA+A+ G E
Sbjct: 422 NPDILDPEERCASTSNRNFEGRQG-ARSRTHLMSPAMAAAAAIKGHFTDIRE 472
>sp|P17279|LEU2_RHIRA 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMALATE ISOMERASE)
(ALPHA-IPM ISOMERASE) (IPMI) >gi|82770|pir||JQ0160
3-isopropylmalate dehydratase (EC 4.2.1.33) - Rhizomucor
circinelloides >gi|168367|gb|AAA33422.1| (M33166)
alpha-isopropylmalate isomerase (put.); putative [Mucor
circinelloides]
Length = 644
Score = 233 bits (589), Expect = 2e-60
Identities = 138/384 (35%), Positives = 210/384 (53%), Gaps = 42/384 (10%)
Query: 71 KDIKSAEQCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPELGLVLPGDLIIGADSHTCTY 129
K+ S QC++ + + G+ +F E G+ H + PE G LP ++ DSHT T+
Sbjct: 128 KEADSRTQCETLEQNIEAFGLTYFGMEDSRQGIVHVIGPEQGFTLPATTVVCGDSHTSTH 187
Query: 130 GALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLILHTIGDIGVN 189
GA GA A G+G++++ +AT + + M+ +G+ P VT KD++LH IG IG
Sbjct: 188 GAFGALAFGIGTSEVEHVLATQTLLQKKSKNMRIRVQGKALPGVTSKDIVLHIIGVIGTA 247
Query: 190 GALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVK---------- 239
G V+EF G I LS+E RM++ NM+IEAGA+ G++ PD+ T +Y++
Sbjct: 248 GGTGCVIEFCGDTIAALSMESRMSICNMSIEAGARAGMVAPDEVTFEYLRDKPLAPKGAD 307
Query: 240 -ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKA----AKM---- 290
+RA + +K SD DAKY +E + + P + + P++ VPI+ + AK+
Sbjct: 308 WDRAVKYWKSLSSDADAKYDINVEINAADIAPTLTWGTSPQDVVPITGSTPDPAKIEDPI 367
Query: 291 ---------------------NIKIDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVRLI 329
+K+D+VFIGSCTN RIEDLR AA +++G++ A+WV +
Sbjct: 368 RRSAVQRALDYIGIAPNTPMEGVKVDKVFIGSCTNSRIEDLRAAAAVVKGKRAAEWVDAM 427
Query: 330 VIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGERAVSTTNRNFVG 389
V+P S V +A +EGL +IF +AG C CLG + L GER ST+NRNF G
Sbjct: 428 VVPGSGLVKRQAEREGLDKIFTDAGFDWREAGCSMCLGMNPDQLKPGERCASTSNRNFEG 487
Query: 390 RMGHPKSEVYLANPYVAAASAVLG 413
R G +L +P +AAA+ + G
Sbjct: 488 RQG-AGGRTHLVSPAMAAAAGIKG 510
>sp|P55811|LEU2_RHINI 3-ISOPROPYLMALATE DEHYDRATASE (ISOPROPYLMALATE ISOMERASE)
(ALPHA-IPM ISOMERASE) (IPMI) >gi|1817566|dbj|BAA09893.1|
(D63833) 3-isopropylmalate dehyratase [Rhizopus niveus]
>gi|1588638|prf||2209244A alpha-isopropylmalate
isomerase [Rhizopus niveus]
Length = 750
Score = 232 bits (586), Expect = 5e-60
Identities = 161/468 (34%), Positives = 234/468 (49%), Gaps = 60/468 (12%)
Query: 1 MGMTIAEKILADHS-EREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRER 59
M T+ +K+L DH +R+E + +D ++VT P A + R G + V +
Sbjct: 1 MARTLYDKVLEDHIIDRQEYGT---CLIYIDRHLVHEVTSPQAFEGLRNAG-RPVRRPDC 56
Query: 60 IAIVLDHFTPN------KDIK-------SAEQCKSSREFAKEMGIKWF-FEGGSVGVEHC 105
+DH P KDI S+ QC++ E + G+ +F E G+ H
Sbjct: 57 TLATVDHNIPTSPRRNFKDIATFIKEGDSSTQCETLGENIEAFGLTYFGMEDSRQGIVHV 116
Query: 106 LLPELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIY 165
+ PE G LPG ++ DSHT T+GA GA A G+G++++ +AT + + M+
Sbjct: 117 IGPEQGFTLPGTTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLLQKKSKNMRIRV 176
Query: 166 EGELQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKT 225
EG+ VT KD+ LH IG IG G V+EF G E LS+E RM++ NM+IEAGA+
Sbjct: 177 EGKPSAGVTSKDIALHVIGVIGTAGGTGCVIEFCGEANESLSMESRMSICNMSIEAGARA 236
Query: 226 GIIEPDKKTLDYV-----------KERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAF 274
G+I PD T +Y+ +RA +K K D DA+Y ++ ++ P V +
Sbjct: 237 GMIAPDDITFEYLCNKPLASKGEEWDRAVAYWKTLKFDADAQYDITVDIKASDIAPTVTW 296
Query: 275 PHLPENTVPI-----------------------------SKAAKMNIKIDQVFIGSCTNG 305
P++ PI S +KID+VFIGSCTN
Sbjct: 297 GTSPQDVAPITGKTPDLDSIADPLRRLAVQRALDYIGIASNTPLEGVKIDKVFIGSCTNS 356
Query: 306 RIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPC 365
RIEDL AA I++G++VA WV +V+P S V +A ++GL +IF EAG C C
Sbjct: 357 RIEDLGPAAAIVKGKRVADWVDAMVVPGSALVKRQAERKGLDKIFQEAGFNWREAGCSMC 416
Query: 366 LGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLG 413
LG + L GER ST+NRNF GR G +L +P +AAA+ + G
Sbjct: 417 LGMNPDQLKPGERCASTSNRNFKGRQG-AGGRTHLLSPAMAAAAGIKG 463
>pir||H82070 3-isopropylmalate dehydratase, large chain VC2492 [similarity] -
Vibrio cholerae (group O1 strain N16961)
>gi|9657072|gb|AAF95634.1| (AE004318) 3-isopropylmalate
dehydratase, large subunit [Vibrio cholerae]
Length = 467
Score = 232 bits (586), Expect = 5e-60
Identities = 158/458 (34%), Positives = 234/458 (50%), Gaps = 52/458 (11%)
Query: 4 TIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIAIV 63
T+ EKI H PGE + +D ++VT P A RE G + V +
Sbjct: 6 TLYEKIYDAHVV--VAAPGETPILYIDRHLVHEVTSPQAFDGLREKG-RPVRQVSKTFAT 62
Query: 64 LDH--FTPNKDIKSAE-----QCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPELGLVLP 115
+DH T KDI ++ Q ++ + +E G+ + G+ H + PELG+ LP
Sbjct: 63 MDHNVSTTTKDINASGEMARIQMQTLSKNCEEFGVTLYDINHKYQGIVHVMGPELGITLP 122
Query: 116 GDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTG 175
G I+ DSHT T+GA G+ A G+G++++ +AT +TMK G++ P +T
Sbjct: 123 GMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQGRAKTMKIEVRGKVAPGITA 182
Query: 176 KDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTL 235
KD++L IG G V+EF G I +LS+E RMT+ NMAIE GAK G+I PD T
Sbjct: 183 KDIVLAIIGKTTAAGGTGYVVEFCGEAIRDLSMEGRMTVCNMAIELGAKAGLIAPDATTF 242
Query: 236 DYVKER-----------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPI 284
+Y+K R A ++ K+DEDA++ V+ + + +P V + P + +
Sbjct: 243 NYIKGRKFAPQGSDWDAAVDYWQTLKTDEDAQFDAVVTLEASEIKPQVTWGTNPGQVIAV 302
Query: 285 SK---------------------------AAKM--NIKIDQVFIGSCTNGRIEDLRMAAE 315
+ A KM + K+D+VF+GSCTN RIED+R AA
Sbjct: 303 DEPIPSPSQFADPVERSSAEKALAYMGLEAGKMLSDYKVDKVFVGSCTNSRIEDMRAAAA 362
Query: 316 ILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLAS 375
+ +G+KVA V+ +++P S V +A EGL +IF+EAG P C CL + L
Sbjct: 363 VAKGKKVASHVQALIVPGSEQVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLGP 422
Query: 376 GERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLG 413
GER ST+NRNF GR G +L +P +AAA+A+ G
Sbjct: 423 GERCASTSNRNFEGRQGR-DGRTHLVSPAMAAAAAIAG 459
>gi|10954452 isopropylmalate isomerase subunit [Buchnera aphidicola]
>gi|3282734|gb|AAD12595.1| (AF041836) isopropylmalate
isomerase subunit [Buchnera aphidicola]
Length = 469
Score = 231 bits (583), Expect = 1e-59
Identities = 156/457 (34%), Positives = 241/457 (52%), Gaps = 52/457 (11%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ EKI H EE I + +D ++VT P A R + + V ++
Sbjct: 1 MKKTLYEKIYDAHIVHEE--KNNISILYIDLHLLHEVTSPQAFDSLR-MRNRSVRQPKKT 57
Query: 61 AIVLDH--FTPNKDIKSAE-----QCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPELGL 112
+DH T +KDI ++ Q + + +E I + G+ H + PE GL
Sbjct: 58 FATMDHNVSTESKDINASGSMAKIQMQQLIKNCEEFNIALYDLNNPKQGIVHVIGPEQGL 117
Query: 113 VLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPY 172
LPG I+ DSHT T+GA GA + G+G++++ + T + + MK G+++ +
Sbjct: 118 TLPGSTIVCGDSHTSTHGAFGALSFGIGTSEVEHVLVTQTLKQQRFKNMKIKIVGKVKKF 177
Query: 173 VTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDK 232
+T KD+IL IG +G + V+EF G VI+++++E+RMT+ NMAIE GAK+ +I PD+
Sbjct: 178 ITAKDIILFIIGKLGTSSGSGYVIEFCGDVIKKMNMEERMTICNMAIEMGAKSALIAPDE 237
Query: 233 KTLDYVKER-----------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENT 281
T Y+K + A R +K +DE+A + K +D+++ P V + P+
Sbjct: 238 TTYLYLKNKRYSPKNKYWDSAIRYWKTLVTDENALFDKEFTFDISDISPQVTWGTSPDQV 297
Query: 282 VPISK-----------------------------AAKMNIKIDQVFIGSCTNGRIEDLRM 312
+ I++ + NIKID+VFIGSCTN RIEDLR
Sbjct: 298 LSINEKIPDFNSFKDSIKKDLARSACNYMDLKPGSYLKNIKIDKVFIGSCTNSRIEDLRS 357
Query: 313 AAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGV 372
AA IL+ +K++K ++ IV+P S +V +A KEGL +IF++AG P C CLG +
Sbjct: 358 AATILKNKKISKNIKAIVVPGSGSVKNQAEKEGLDKIFIDAGFEWRLPGCSMCLGMNNDK 417
Query: 373 LASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAAS 409
L+ GER ST+NRNF GR G +L +P +AAA+
Sbjct: 418 LSDGERCASTSNRNFEGRQGR-GGRTHLVSPIMAAAA 453
>gb|AAG31406.1| (AF197457) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 448
Score = 231 bits (582), Expect = 2e-59
Identities = 155/454 (34%), Positives = 240/454 (52%), Gaps = 58/454 (12%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ +KI H EE ++ L + ++V P A R+ ++V ++
Sbjct: 1 MKKTLYDKIYDSHIVYEEKNNTSLLYIDLHLL--HEVASPQAFDSLRDKN-RKVRQPKKT 57
Query: 61 AIVLDH--FTPNKDIKSA---------EQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPE 109
+DH T ++DI ++ E K+ EF + + + + G+ H + PE
Sbjct: 58 FATMDHNVSTTSQDINASGSMAKVQMQELIKNCSEFNISL---YDIKNPNQGIVHVISPE 114
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGEL 169
G+ LPG I+ DSHT T+GA GA + G+G++++ +AT + + MK GE+
Sbjct: 115 KGMTLPGMTIVCGDSHTSTHGAFGALSFGIGTSEVEHVLATQTLKQQRFKNMKIEITGEI 174
Query: 170 QPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIE 229
Q +VT KDLIL IG +G +G ++EF G+VIE++S+E+RMT+ NMAIE GAK+G+I
Sbjct: 175 QKFVTAKDLILFIIGKLGSSGGAGYIIEFCGNVIEKMSMEERMTICNMAIEIGAKSGLIA 234
Query: 230 PDKKTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLP 278
PD+ T Y+K ++A +K KSD++A + KV+ ++++ P + + P
Sbjct: 235 PDEVTFSYLKNKMYAPRGVFWKKALNFWKNLKSDKNAFFDKVVNINISDLSPQITWGTNP 294
Query: 279 ENTVPISK-------------------AAKM----------NIKIDQVFIGSCTNGRIED 309
+ + I + A K NI +D+VFIGSCTNGRIED
Sbjct: 295 DQVISIDQKIPDFSSFDNLIKKDLAKSACKYMGLKIGTYLTNITVDKVFIGSCTNGRIED 354
Query: 310 LRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGH 369
LR A++IL+ +K+A V+ IV+P S +V +A EGL +IF+ AG P C CLG +
Sbjct: 355 LRAASKILKDKKIANNVKAIVVPGSGSVKREAENEGLDKIFINAGFEWRLPGCSMCLGMN 414
Query: 370 MGVLASGERAVSTTNRNFVGRMGHPKSEVYLANP 403
L GER S +NRNF GR G +L +P
Sbjct: 415 KDRLNDGERCASHSNRNFEGRQGR-XXRTHLVSP 447
>sp|P30127|LEU2_ECOLI 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
Length = 466
Score = 230 bits (580), Expect = 3e-59
Identities = 167/467 (35%), Positives = 232/467 (48%), Gaps = 58/467 (12%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ EK+ H E E + +D ++VT P A R G + V +
Sbjct: 1 MAKTLYEKLFDAHVVYEA--ENETPLLYIDRHLVHEVTSPQAFDGLRAHG-RPVRQPGKT 57
Query: 61 AIVLDH--FTPNKDIKSA---------EQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPE 109
+DH T KDI + E K+ +EF E+ + G+ H + PE
Sbjct: 58 FATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVEL---YDLNHPYQGIVHVMGPE 114
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGEL 169
G+ LPG I+ DSHT T+GA GA A G+G++++ +AT +TMK +G+
Sbjct: 115 QGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVQGKA 174
Query: 170 QPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIE 229
P +T KD++L IG G G V+EF G I +LS+E RMT+ NMAIE GAK G++
Sbjct: 175 APGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVA 234
Query: 230 PDKKTLDYVKER-----------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLP 278
PD+ T +YVK R A +K ++DE A + V+ P V + P
Sbjct: 235 PDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEISPQVTWGTNP 294
Query: 279 ENTV--------------PISKA------AKMNIK---------IDQVFIGSCTNGRIED 309
+ P+ +A A M +K ID+VFIGSCTN RIED
Sbjct: 295 GQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPLTEVAIDKVFIGSCTNSRIED 354
Query: 310 LRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGH 369
LR AAEI++G+KVA V+ +V+P S V +A EGL +IF+EAG P C CL +
Sbjct: 355 LRAAAEIVKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMN 414
Query: 370 MGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIA 416
L GER ST+NRNF GR G +L +P +AAA+AV G A
Sbjct: 415 NDRLNPGERCASTSNRNFEGRQGR-GGRTHLVSPAMAAAAAVTGHFA 460
>gb|AAB19612.1| (S57886) isopropylmalate isomerase {EC 4.2.1.33} [Saccharomyces
cerevisiae, Peptide, 782 aa]
Length = 782
Score = 229 bits (579), Expect = 3e-59
Identities = 155/444 (34%), Positives = 227/444 (50%), Gaps = 60/444 (13%)
Query: 35 NDVTMPLAIKKFRELGVKRVFDRERIAIVLDHFTPN---KDIKSAE----------QCKS 81
++VT P A + G ++V + +DH P K+ KS + Q K+
Sbjct: 40 HEVTSPQAFEGLENAG-RKVRRVDCTLATVDHNIPTESRKNFKSLDTFIKQTDSRLQVKT 98
Query: 82 SREFAKEMGIKWF-FEGGSVGVEHCLLPELGLVLPGDLIIGADSHTCTYGALGAFATGVG 140
K+ G+ +F G+ H + PE G LPG ++ DSHT T+GA G+ A G+G
Sbjct: 99 LENNVKQFGVPYFGMSDARQGIVHTIGPEEGFTLPGTTVVCGDSHTSTHGAFGSLAFGIG 158
Query: 141 STDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLILHTIGDIGVNGALYKVMEFSG 200
++++ +AT + M+ G+L P +T KDLIL+ IG IG G V+EF+G
Sbjct: 159 TSEVEHVLATQTIIQAKSKNMRITVNGKLSPGITSKDLILYIIGLIGTAGGTGCVIEFAG 218
Query: 201 SVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVK-----------ERAKRKFKVY 249
IE LS+E RM+M NMAIEAGA+ G+I+PD+ T Y K E+A +K
Sbjct: 219 EAIEALSMEARMSMCNMAIEAGARAGMIKPDETTFQYTKGRPLAPKGAEWEKAVAYWKTL 278
Query: 250 KSDEDAKYYKVI---EYDVTNWEPVVAFPHLPENTVPISKAAK----------------- 289
K+DE AK+ I + + P + + P++ +PI+ +
Sbjct: 279 KTDEGAKFDHEITLKHIEAVDVIPTITWGTSPQDALPITGSVPDPKNVTDPIKKSGMERA 338
Query: 290 ------------MNIKIDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVRL-IVIPCSPT 336
+IK+D+VFIGSCTNGRIEDLR AA ++ GQK+A ++L +V+P S
Sbjct: 339 LAYMGLEPNTPLKSIKVDKVFIGSCTNGRIEDLRSAAAVVRGQKLASNIKLAMVVPGSGL 398
Query: 337 VYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKS 396
V +A EGL +IF EAG C CLG + +L + ER ST+NRNF GR G S
Sbjct: 399 VKKQAEAEGLDKIFQEAGFEWREAGCSMCLGMNPDILDAYERCASTSNRNFEGRQG-ALS 457
Query: 397 EVYLANPYVAAASAVLGRIASPEE 420
+L +P +AAA+ + G E
Sbjct: 458 RTHLISPAMAAAAGIAGHFVDIRE 481
>gb|AAG31388.1| (AF197451) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 442
Score = 229 bits (579), Expect = 3e-59
Identities = 152/440 (34%), Positives = 230/440 (51%), Gaps = 51/440 (11%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ +KI H E+ K ++ L + ++VT P A R ++V E+
Sbjct: 1 MSKTLYQKIYDSHIVYEDKKNISVLYIDLHLI--HEVTSPQAFDSLRNKK-RKVRQPEKT 57
Query: 61 AIVLDH--FTPNKDIKSA-----EQCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPELGL 112
+DH T +DI ++ +Q + E I + + G+ H + PE G+
Sbjct: 58 FATMDHNVSTQKRDINASGSMAKKQMQQLIRNCDEFNISLYDINNPNQGIVHVIAPEKGM 117
Query: 113 VLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPY 172
LPG +I+ DSHT T+GA GA + G+G++++ +AT + + MK G++ +
Sbjct: 118 TLPGMIIVCGDSHTSTHGAFGALSFGIGTSEVEHVLATQTLKQQCFKNMKVEIVGDIPKF 177
Query: 173 VTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDK 232
VT KD+IL I +G +G V+EF G+VIE++S+E+RMT+ NMAIE GAK+GII PD+
Sbjct: 178 VTAKDIILFIIRRLGSSGGSGYVIEFCGNVIEKMSMEERMTVCNMAIEMGAKSGIIAPDE 237
Query: 233 KTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENT 281
T Y+K E + +K KSD+DA + K D++N P V + P+
Sbjct: 238 TTYAYLKNKIYSPSGACWEXSISYWKTLKSDKDAFFDKCFTIDISNLAPQVTWGTSPDQV 297
Query: 282 VPISK-----------------------------AAKMNIKIDQVFIGSCTNGRIEDLRM 312
+ I++ A NI ID+VFIGSCTN RIEDLR
Sbjct: 298 ISINEKIPHYNEFNSITQKNSAKSALKYMGLNEGAYLTNISIDKVFIGSCTNARIEDLRS 357
Query: 313 AAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGV 372
A++IL+ +K++ V+ IV+P S +V +A +EGL +IF+ +G P C CLG +
Sbjct: 358 ASKILKNRKISSNVKAIVVPGSGSVKQQAEREGLDKIFINSGFEWRLPGCSMCLGMNRDQ 417
Query: 373 LASGERAVSTTNRNFVGRMG 392
L GER S +NRNF GR G
Sbjct: 418 LNIGERCASXSNRNFEGRQG 437
>sp|P44968|LEU2_HAEIN 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|1074594|pir||F64163 3-isopropylmalate dehydratase
(EC 4.2.1.33) chain leuC - Haemophilus influenzae
(strain Rd KW20) >gi|1574017|gb|AAC22649.1| (U32779)
3-isopropylmalate dehydratase, alpha subunit (leuC)
[Haemophilus influenzae Rd]
Length = 469
Score = 229 bits (578), Expect = 4e-59
Identities = 165/470 (35%), Positives = 238/470 (50%), Gaps = 53/470 (11%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ EK+ H E GE + ++ ++VT P A R + ++V +
Sbjct: 1 MAKTLYEKLFDSHIVYEA--EGETPILYINRHLIHEVTSPQAFDGLR-VANRQVRQVNKT 57
Query: 61 AIVLDHF--TPNKDIKSAE-----QCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPELGL 112
+DH T +D+ E Q + K GIK F G+ H + PE GL
Sbjct: 58 FGTMDHSISTQVRDVNKLEGQAKIQVLELDKNTKATGIKLFDITTKEQGIVHVMGPEQGL 117
Query: 113 VLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPY 172
LPG I+ DSHT T+GA GA A G+G++++ +AT ++MK G++
Sbjct: 118 TLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQARAKSMKIEVRGKVASG 177
Query: 173 VTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDK 232
+T KD+IL IG + G V+EF G I++LS+E RMT+ NMAIE GAK G+I PD+
Sbjct: 178 ITAKDIILAIIGKTTMAGGTGHVVEFCGEAIQDLSMEGRMTVCNMAIEMGAKAGLIAPDE 237
Query: 233 KTLDYVKER-----------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAF------- 274
T Y+K+R A +K KSD+DA++ V+ + + P V +
Sbjct: 238 TTFAYLKDRPHAPKGKDWEDAVAYWKTLKSDDDAQFDTVVTLEAKDIAPQVTWGTNPGQV 297
Query: 275 -------PHLPENTVPISKAAK---------------MNIKIDQVFIGSCTNGRIEDLRM 312
P+ E P+ +A+ +IK+DQVFIGSCTN RIEDLR
Sbjct: 298 ISVNETIPNPQEMADPVQRASAEKALHYIGLEAGTNLKDIKVDQVFIGSCTNSRIEDLRA 357
Query: 313 AAEILEGQKVAKWV-RLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMG 371
AA +++G+K A V R++V+P S V +A KEGL +IF+ AGA P C CLG +
Sbjct: 358 AAAVMKGRKKADNVKRILVVPGSGLVKEQAEKEGLDKIFIAAGAEWRNPGCSMCLGMNDD 417
Query: 372 VLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEEV 421
L ER ST+NRNF GR G +L +P +AAA+ V G+ +V
Sbjct: 418 RLGEWERCASTSNRNFEGRQGR-NGRTHLVSPAMAAAAGVFGKFVDIRDV 466
>sp|Q92412|LYS4_EMENI HOMOACONITASE PRECURSOR (HOMOACONITATE HYDRATASE)
>gi|1628374|emb|CAA67943.1| (X99624) homoaconitase
[Aspergillus nidulellus]
Length = 775
Score = 229 bits (578), Expect = 4e-59
Identities = 145/455 (31%), Positives = 234/455 (50%), Gaps = 43/455 (9%)
Query: 4 TIAEKILADHS----EREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRER 59
T+ EKI+ +S + + VK G+ V + +D + P A+K F +G R+ + ++
Sbjct: 67 TLTEKIVQKYSVGLPQGKFVKSGDYVTIQPHRCMTHDNSWPCALK-FMSIGASRLHNPDQ 125
Query: 60 IAIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLI 119
I + LDH NK K+ ++ + EFA + G++++ G +G H ++ E G PG L
Sbjct: 126 IVMTLDHDVQNKSDKNLKKYRQIEEFATQHGVEFYPAGRGIG--HQIMIEEGFAWPGTLA 183
Query: 120 IGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLI 179
+ +DSH+ YG +G T + TD A ATG+ W+++P K ++G L P VTGKD+I
Sbjct: 184 VASDSHSNMYGGVGCLGTPIVRTDAASVWATGKTWWQIPPVAKVTFKGVLPPGVTGKDVI 243
Query: 180 LHTIGDIGVNGALYKVMEFSGS--VIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDY 237
+ G + L +EF+GS + LSV+ R+T++NM E GA +G+ D +
Sbjct: 244 VALCGLFNKDDVLNHAIEFTGSEETMRSLSVDTRLTIANMTTEWGALSGLFPIDSVLKGW 303
Query: 238 V----------------KERAKRKF----------KVYKSDEDAKYYKVIEYDVTNWEPV 271
+ K RA +F +D+ AKY K + D+++ P
Sbjct: 304 LRGKATTAAMGLADGPFKTRAAERFTHPLLEQLFENPLTADKGAKYAKELFLDLSSLSPY 363
Query: 272 VAFPHLPENTVPISKAAKMNIKIDQVFIGSCTNGRIEDLRMAAEILE------GQKVAK- 324
V+ P+ + P+ + NIK+D+ ++ SCTN R D+ AA++ + G K+ K
Sbjct: 364 VSGPNSVKVATPLKELEAQNIKVDKAYLVSCTNSRASDIAAAAKVFKEAAEKNGGKIPKI 423
Query: 325 -WVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGERAVSTT 383
V+ + S A G + LEAGA P CGPC+G G+L GE +S +
Sbjct: 424 HGVKFYIAAASIPEQLAAEGNGDWQTLLEAGATQLPAGCGPCIGMGQGLLEPGEVGISAS 483
Query: 384 NRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASP 418
NRNF GRMG +++ YL +P V AASA+ G+++ P
Sbjct: 484 NRNFKGRMGSTEAKAYLGSPEVVAASALSGKLSGP 518
>pir||H64728 3-isopropylmalate dehydratase (EC 4.2.1.33) alpha chain -
Escherichia coli >gi|1786259|gb|AAC73183.1| (AE000117)
3-isopropylmalate isomerase (dehydratase) subunit
[Escherichia coli K12]
Length = 466
Score = 229 bits (578), Expect = 4e-59
Identities = 167/467 (35%), Positives = 231/467 (48%), Gaps = 58/467 (12%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ EK+ H E E + +D ++VT P A R G + V +
Sbjct: 1 MAKTLYEKLFDAHVVYEA--ENETPLLYIDRHLVHEVTSPQAFDGLRAHG-RPVRQPGKT 57
Query: 61 AIVLDH--FTPNKDIKSA---------EQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPE 109
+DH T KDI + E K+ +EF E+ + G+ H + PE
Sbjct: 58 FATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVEL---YDLNHPYQGIVHVMGPE 114
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGEL 169
G+ LPG I+ DSHT T+GA GA A G+G++++ +AT +TMK +G+
Sbjct: 115 QGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVQGKA 174
Query: 170 QPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIE 229
P +T KD++L IG G G V+EF G I +LS+E RMT+ NMAIE GAK G++
Sbjct: 175 APGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVA 234
Query: 230 PDKKTLDYVKER-----------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLP 278
PD+ T +YVK R A +K ++DE A + V+ P V + P
Sbjct: 235 PDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEISPQVTWGTNP 294
Query: 279 ENTV--------------PISKA------AKMNIK---------IDQVFIGSCTNGRIED 309
+ P+ +A A M +K ID+VFIGSCTN RIED
Sbjct: 295 GQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPLTEVAIDKVFIGSCTNSRIED 354
Query: 310 LRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGH 369
LR AAEI +G+KVA V+ +V+P S V +A EGL +IF+EAG P C CL +
Sbjct: 355 LRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMN 414
Query: 370 MGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIA 416
L GER ST+NRNF GR G +L +P +AAA+AV G A
Sbjct: 415 NDRLNPGERCASTSNRNFEGRQGR-GGRTHLVSPAMAAAAAVTGHFA 460
>sp|Q02142|LEU2_LACLA 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|486726|pir||S35134 probable 3-isopropylmalate
dehydratase (EC 4.2.1.33) chain leuC - Lactococcus
lactis subsp. lactis >gi|2565153|gb|AAB81915.1| (U92974)
LeuC [Lactococcus lactis]
Length = 460
Score = 228 bits (575), Expect = 1e-58
Identities = 156/461 (33%), Positives = 232/461 (49%), Gaps = 45/461 (9%)
Query: 2 GMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIA 61
G TI +K+ H GE + +D ++VT P A + RE G +RV ++
Sbjct: 3 GKTIFDKLWDQHVIAGN--EGEPQLLYIDLHVIHEVTSPQAFQGLREAG-RRVRRKDLTY 59
Query: 62 IVLDHFTPNKDIKSAEQCKSSREF------AKEMGIKWFFEGGS-VGVEHCLLPELGLVL 114
LDH P ++I + + S ++ KE + GG G+ H + PE G
Sbjct: 60 GTLDHNVPTQNIFNIQDLISKKQIDTFTKNVKEFDVPAETHGGKGQGIVHMVAPESGRTQ 119
Query: 115 PGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVT 174
PG I+ DSHT T GA GA A G+G++++ +AT W P+ MK ++G Q +
Sbjct: 120 PGKTIVCGDSHTATNGAFGAIAFGIGTSEVEHVLATQTIWQVKPKRMKIEFQGHPQKGIY 179
Query: 175 GKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKT 234
KD IL I GV+ + +E+SG I +LS+E+RMT+ NM+IE GAK G++ PD+KT
Sbjct: 180 SKDFILALIAKYGVDAGVGYAVEYSGDAISDLSMEERMTICNMSIEFGAKIGLMNPDEKT 239
Query: 235 LDYVKER---------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVA------------ 273
DYVK R A K++ SD DA+Y K++ DV+ +P+V
Sbjct: 240 YDYVKGREHAPKNFDEAVSKWEKLVSDSDAQYDKILSLDVSQLKPMVTWGTNPGMGLEFG 299
Query: 274 --FPHLPENTVPISKA----------AKMNIKIDQVFIGSCTNGRIEDLRMAAEILEGQK 321
FP + N + +A +I + +FIGSCTN R+ DL AA+I+ +
Sbjct: 300 EKFPEI-NNDLNYERAYQYMDLKPGQTASDIDLGYIFIGSCTNARLGDLEEAAKIIGDRH 358
Query: 322 VAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGERAVS 381
+A + IV+P S V A +GL +IF EAG P C CLG + + S
Sbjct: 359 IADGLTGIVVPGSRPVKEAAEAQGLDKIFKEAGFEWREPGCSACLGMNPDQIPEYVHCAS 418
Query: 382 TTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEEVV 422
T+NRNF GR GH + +L +P +AAA+A+ G+ +V
Sbjct: 419 TSNRNFEGRQGH-NARTHLCSPAMAAAAAIAGKFVDVRMLV 458
>pir||S40586 3-isopropylmalate dehydratase (EC 4.2.1.33) alpha chain -
Escherichia coli >gi|216490|dbj|BAA01341.1| (D10483)
isopropylmalate isomerase chain leuC homolog(PIR:S10171)
[Escherichia coli]
Length = 466
Score = 227 bits (573), Expect = 2e-58
Identities = 166/467 (35%), Positives = 231/467 (48%), Gaps = 58/467 (12%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ EK+ H E E + +D ++VT P A R G + V +
Sbjct: 1 MAKTLYEKLFDAHVVYEA--ENETPLLYIDRHLVHEVTSPQAFDGLRAHG-RPVRQPGKT 57
Query: 61 AIVLDH--FTPNKDIKSA---------EQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPE 109
+DH T K I + E K+ +EF E+ + G+ H + PE
Sbjct: 58 FATMDHNVSTQTKGINACGEMARIQMQELIKNCKEFGVEL---YDLNHPYQGIVHVMGPE 114
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGEL 169
G+ LPG I+ DSHT T+GA GA A G+G++++ +AT +TMK +G+
Sbjct: 115 QGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVQGKA 174
Query: 170 QPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIE 229
P +T KD++L IG G G V+EF G I +LS+E RMT+ NMAIE GAK G++
Sbjct: 175 APGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVA 234
Query: 230 PDKKTLDYVKER-----------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLP 278
PD+ T +YVK R A +K ++DE A + V+ P V + P
Sbjct: 235 PDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEISPQVTWGTNP 294
Query: 279 ENTV--------------PISKA------AKMNIK---------IDQVFIGSCTNGRIED 309
+ P+ +A A M +K ID+VFIGSCTN RIED
Sbjct: 295 GQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPLTEVAIDKVFIGSCTNSRIED 354
Query: 310 LRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGH 369
LR AAEI++G+KVA V+ +V+P S V +A EGL +IF+EAG P C CL +
Sbjct: 355 LRAAAEIVKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMN 414
Query: 370 MGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIA 416
L GER ST+NRNF GR G +L +P +AAA+AV G A
Sbjct: 415 NDRLNPGERCASTSNRNFEGRQGR-GGRTHLVSPAMAAAAAVTGHFA 460
>gb|AAG31397.1| (AF197454) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 444
Score = 227 bits (573), Expect = 2e-58
Identities = 154/438 (35%), Positives = 228/438 (51%), Gaps = 53/438 (12%)
Query: 4 TIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIAIV 63
T+ +KI H E+ K GE ++ +D ++VT P A R ++V ++
Sbjct: 5 TLYQKIYDSHVVYED-KNGESILY-IDLHLLHEVTSPQAFDALRSKK-RKVRQSKKTFAT 61
Query: 64 LDHFTPNKDIKSAEQCKSSREFAKEMGIK---------WFFEGGSVGVEHCLLPELGLVL 114
+DH K I+S S + E IK + + G+ H + PE G+ L
Sbjct: 62 MDHNVSTK-IQSISASGSMAKKQMEQLIKNCRDFNIPLYDINNPNQGIVHVIAPEKGMTL 120
Query: 115 PGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVT 174
PG I+ DSHT T+GA GA A G+G++++ +AT + + MK G++ +VT
Sbjct: 121 PGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLKQKRFKNMKVEIIGKIPKFVT 180
Query: 175 GKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKT 234
KD+IL IG +G + V+EF G VI+ +S+E+RMT+ NMAIE GAK+G+I PD+ T
Sbjct: 181 AKDIILFIIGQLGSSSGTGYVIEFCGDVIKNISMEERMTICNMAIEMGAKSGLIAPDEIT 240
Query: 235 LDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVP 283
Y+K E++ +K KSD++A + K I D++N P + + P+ +
Sbjct: 241 YKYLKDKIYSPSGLFWEKSLDYWKFLKSDKNAHFDKCITLDISNLAPQITWGTNPDQVIS 300
Query: 284 I-----------------------------SKAAKMNIKIDQVFIGSCTNGRIEDLRMAA 314
I S NI ID+VFIGSCTN RIEDLR A+
Sbjct: 301 IDEKIPDYNNINSLLKKESAKSACEYMGLKSNTYLTNISIDRVFIGSCTNARIEDLRAAS 360
Query: 315 EILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLA 374
+IL+ +K+AK V+ IV+P S +V KA +EGL +IF+++G P C CLG + L
Sbjct: 361 KILKNRKIAKHVKAIVVPGSGSVKRKAEQEGLDKIFIDSGFEWRLPGCSMCLGMNKDRLN 420
Query: 375 SGERAVSTTNRNFVGRMG 392
GER S +NRNF GR G
Sbjct: 421 FGERCASHSNRNFEGRQG 438
>pir||G81128 3-isopropylmalate dehydratase, large chain NMB1036 [imported] -
Neisseria meningitidis (group B strain MD58)
>gi|7226276|gb|AAF41435.1| (AE002454) 3-isopropylmalate
dehydratase, large subunit [Neisseria meningitidis MC58]
Length = 469
Score = 227 bits (572), Expect = 2e-58
Identities = 159/461 (34%), Positives = 235/461 (50%), Gaps = 54/461 (11%)
Query: 4 TIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIAIV 63
T+ +K+ H REE V+ +D ++VT P A + + G ++++ + +
Sbjct: 5 TLYDKLWNSHVVREE--EDGTVLLYIDRHLVHEVTSPQAFEGLKMAG-RKLWRIDSVVST 61
Query: 64 LDHFTPNKDIKSAEQCKSSR-------EFAKEMGIKWFFE--GGSVGVEHCLLPELGLVL 114
DH TP D Q S+ + KE G +F G+ H + PE G L
Sbjct: 62 ADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPFMDKGQGIVHVMGPEQGATL 121
Query: 115 PGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVT 174
PG ++ DSHT T+GA GA A G+G++++ MAT + ++M +G+L+ VT
Sbjct: 122 PGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCITAKKSKSMLISVDGKLKAGVT 181
Query: 175 GKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKT 234
KD+ L+ IG IG G +EF G I LS+E RMT+ NMAIEAGA++G++ D+ T
Sbjct: 182 AKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMESRMTLCNMAIEAGARSGMVAVDQTT 241
Query: 235 LDYVKER-----------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAF--------- 274
+DYVK++ A ++ SDE A + K ++ + EP V +
Sbjct: 242 IDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFDKEYRFNAEDIEPQVTWGTSPEMVLD 301
Query: 275 -----PHLPENTVPISKAAKMN---------------IKIDQVFIGSCTNGRIEDLRMAA 314
P+ E T P+ ++ I +D VFIGSCTN RIEDLR AA
Sbjct: 302 ISSKVPNPAEETDPVKRSGMERALEYMGLEAGTPLNEIPVDIVFIGSCTNSRIEDLREAA 361
Query: 315 EILEGQKVAKWV-RLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVL 373
I + +K A V R++++P S V +A KEGL +IF+EAG P C CL + L
Sbjct: 362 AIAKDRKKAANVQRVLIVPGSGLVKEQAEKEGLDKIFIEAGFEWREPGCSMCLAMNADRL 421
Query: 374 ASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGR 414
G+R ST+NRNF GR G+ +L +P +AAA+AV GR
Sbjct: 422 TPGQRCASTSNRNFEGRQGN-GGRTHLVSPAMAAAAAVTGR 461
>pir||A81270 3-isopropylmalate dehydratase (EC 4.2.1.33) large chain Cj1717c
[imported] - Campylobacter jejuni (strain NCTC 11168)
>gi|6969132|emb|CAB73703.1| (AL139079) 3-isopropylmalate
dehydratase large subunit [Campylobacter jejuni]
Length = 470
Score = 226 bits (571), Expect = 3e-58
Identities = 160/463 (34%), Positives = 235/463 (50%), Gaps = 52/463 (11%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ EK+ H E E+ + +D ++VT P A + + +R+ +
Sbjct: 3 MAKTLYEKVFDAHVVYEG--KNELPILYIDRHLIHEVTSPQAFSGLK-MAKRRMARADLT 59
Query: 61 AIVLDHFTPNKDIK-------SAEQCKSSREFAKEMGIKWFFEGG-SVGVEHCLLPELGL 112
+DH K + + EQ + + KE G++ G + G+ H + PELG
Sbjct: 60 LATIDHDVSTKSMDLNACSDMAKEQITTLMQNTKEFGVRLLGLGDKNQGIVHIVGPELGF 119
Query: 113 VLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPY 172
LPG ++ DSHT T+GA GA A G+G++++ MAT +TMK +G+ Q
Sbjct: 120 TLPGVTLVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLKQAKLKTMKIECKGQFQKG 179
Query: 173 VTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDK 232
V KDLIL+ I G +EF G +I LS+E RMT+ NMAIE GAK G+I PD+
Sbjct: 180 VYTKDLILYLIAQYGTAKGTGYAIEFCGELIRNLSMEARMTLCNMAIEFGAKVGMIAPDE 239
Query: 233 KTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENT 281
T +Y+K ++ +K +SDE AKY I DV+ +P +++ P
Sbjct: 240 ITFEYIKGKEFAPKGEEFQKYCEYWKSLRSDEGAKYDASITLDVSKIKPQISYGTNPSQV 299
Query: 282 VPI-SKAAKMN----------------------------IKIDQVFIGSCTNGRIEDLRM 312
+ I K K++ +KID VFIGSCTNGR+EDL++
Sbjct: 300 IGIDEKIPKISDFKNQSEQKSLLDALYYVNLEQDQVIEGVKIDIVFIGSCTNGRLEDLKI 359
Query: 313 AAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGV 372
AA+IL+G+K+ K V+ +++P S V +A GL +IF+EAG C CLG +
Sbjct: 360 AADILKGRKIHKNVKALIVPGSMQVRKEAENLGLDKIFIEAGCEWRYAGCSMCLGMNDDK 419
Query: 373 LASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRI 415
SG+R ST+NRNFVGR G S +L +P AAA A+ G I
Sbjct: 420 ANSGQRVASTSNRNFVGRQG-KGSITHLMSPASAAACAIEGVI 461
>pir||A81836 probable 3-isopropylmalate dehydratase (EC 4.2.1.33) large chain
NMA1450 [imported] - Neisseria meningitidis (group A
strain Z2491) >gi|7380103|emb|CAB84686.1| (AL162756)
putative 3-isopropylmalate dehydratase large subunit
[Neisseria meningitidis Z2491]
Length = 469
Score = 226 bits (570), Expect = 4e-58
Identities = 158/461 (34%), Positives = 235/461 (50%), Gaps = 54/461 (11%)
Query: 4 TIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIAIV 63
T+ +K+ H REE V+ +D ++VT P A + + G ++++ + +
Sbjct: 5 TLYDKLWNSHVVREE--EDGTVLLYIDRHLVHEVTSPQAFEGLKMAG-RKLWRIDSVVST 61
Query: 64 LDHFTPNKDIKSAEQCKSSR-------EFAKEMGIKWFFE--GGSVGVEHCLLPELGLVL 114
DH TP D Q S+ + KE G +F G+ H + PE G L
Sbjct: 62 ADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPFMDKGQGIVHVMGPEQGATL 121
Query: 115 PGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVT 174
PG ++ DSHT T+GA GA A G+G++++ MAT + ++M +G+L+ VT
Sbjct: 122 PGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCITAKKSKSMLIAVDGKLKAGVT 181
Query: 175 GKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKT 234
KD+ L+ IG IG G +EF G I LS+E RMT+ NMAIEAGA++G++ D+ T
Sbjct: 182 AKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMEGRMTLCNMAIEAGARSGMVAVDQTT 241
Query: 235 LDYVKER-----------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAF--------- 274
+DYVK++ A ++ SDE A + K ++ + EP V +
Sbjct: 242 IDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFDKEYRFNAEDIEPQVTWGTSPEMVLD 301
Query: 275 -----PHLPENTVPISKAAKMN---------------IKIDQVFIGSCTNGRIEDLRMAA 314
P+ E T P+ ++ I +D VFIGSCTN R+EDLR AA
Sbjct: 302 ISSKVPNPAEETDPVKRSGMERALEYMGLEAGTPLNEIPVDIVFIGSCTNSRVEDLREAA 361
Query: 315 EILEGQKVAKWV-RLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVL 373
I + +K A V R++++P S V +A KEGL +IF+EAG P C CL + L
Sbjct: 362 AIAKDRKKAANVQRVLIVPGSGLVKEQAEKEGLDKIFIEAGFEWREPGCSMCLAMNADRL 421
Query: 374 ASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGR 414
G+R ST+NRNF GR G+ +L +P +AAA+AV GR
Sbjct: 422 TPGQRCASTSNRNFEGRQGN-GGRTHLVSPAMAAAAAVTGR 461
>gb|AAG31400.1| (AF197455) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 443
Score = 226 bits (569), Expect = 5e-58
Identities = 152/441 (34%), Positives = 226/441 (50%), Gaps = 53/441 (12%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ +KI H E+ I+ L + ++VT P A R ++V ++
Sbjct: 1 MSKTLYQKIYDSHIVYEDKNSEAILYIDLHLL--HEVTSPQAFNALRNKK-RKVRQSKKT 57
Query: 61 AIVLDHFTPNKDIKSAEQCKSSREFAKEMGIK---------WFFEGGSVGVEHCLLPELG 111
+DH K I+S S + E I + + G+ H + PE G
Sbjct: 58 FATMDHNVSTK-IRSIHASGSMAQKQMEQLINNCTEFNIPLYDINNPNQGIVHVIAPEKG 116
Query: 112 LVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQP 171
+ LPG I+ DSHT T+GA G A G+G++++ +AT + + MK GE+
Sbjct: 117 MTLPGMTIVCGDSHTSTHGAFGTLAFGIGTSEVEHVLATQTLKQKRFKNMKVEIIGEMPK 176
Query: 172 YVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPD 231
+VT KD+IL IG +G + V+EF G+VI+ +S+E+RMT+ NMA+E GAK+G+I PD
Sbjct: 177 FVTAKDIILFIIGKLGSSSGAGHVIEFCGNVIKNMSMEERMTICNMAVEMGAKSGLIAPD 236
Query: 232 KKTLDYVKERAKRKFKVY-----------KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ T Y+K++ F ++ KSD++A + K + D++N P + + P+
Sbjct: 237 EITYKYLKDKIYSPFGIFWEKSLDYWKFLKSDKNAYFDKCVTVDISNLAPQITWGTNPDQ 296
Query: 281 TVPIS---------------KAAK--------------MNIKIDQVFIGSCTNGRIEDLR 311
+ I K+AK NI ID+VFIGSCTN RIEDLR
Sbjct: 297 VISIDEKIPDYNNINSIVKRKSAKSACEYMGLQSNTYLTNISIDKVFIGSCTNARIEDLR 356
Query: 312 MAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMG 371
A++IL+ +KVAK V IV+P S V +A +EGL +IF++AG P C CLG +
Sbjct: 357 SASKILKNKKVAKHVTAIVVPGSGLVKNQAEEEGLDKIFIDAGFEWRLPGCSMCLGMNKD 416
Query: 372 VLASGERAVSTTNRNFVGRMG 392
L GER S +NRNF GR G
Sbjct: 417 RLNFGERCASHSNRNFEGRQG 437
>pir||G83255 3-isopropylmalate dehydratase large subunit PA3121 [imported] -
Pseudomonas aeruginosa (strain PAO1)
>gi|9949233|gb|AAG06509.1|AE004736_6 (AE004736)
3-isopropylmalate dehydratase large subunit [Pseudomonas
aeruginosa]
Length = 474
Score = 224 bits (566), Expect = 1e-57
Identities = 167/475 (35%), Positives = 238/475 (49%), Gaps = 58/475 (12%)
Query: 2 GMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIA 61
G T+ +K+ H ++ ++ +D ++VT P A + R G K IA
Sbjct: 3 GKTLYDKLWDMHLVKQRDDGSALIY--IDRHILHEVTSPQAFEGLRLAGRKPWRIDANIA 60
Query: 62 IVLDHFTPNK------------DIKSAEQCKSSREFAKEMGIKWF-FEGGSVGVEHCLLP 108
DH P D S Q ++ E + GI F G+ H + P
Sbjct: 61 TP-DHNVPTTRTERKGGIAAIADEVSRLQVQTLDENCDDFGITEFKMNDVRQGIVHVVGP 119
Query: 109 ELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGE 168
E G LPG ++ DSHT T+GA GA A G+G++++ +AT + + M EG
Sbjct: 120 EQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLVAKKMKNMLVKVEGR 179
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L VT KD++L IG IG G +EF+GS I +LS+E RMT+ NM+IEAGA+ G++
Sbjct: 180 LPAGVTAKDIVLAVIGRIGTAGGNGHAIEFAGSAIRDLSIEGRMTICNMSIEAGARVGLV 239
Query: 229 EPDKKTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHL 277
D+KT+DYVK ++A ++ SD DA++ V+E D +P V++
Sbjct: 240 AVDQKTIDYVKGRPFAPSAEQWDQAVACWQGLVSDADARFDTVVELDAAQIKPQVSWGTS 299
Query: 278 PE------NTVP-------------ISKAAK----------MNIKIDQVFIGSCTNGRIE 308
PE VP I +A K +I++D+VFIGSCTN RIE
Sbjct: 300 PEMVLAVDQNVPDPARESDPIKRGSIERALKYMGLRPNQAITDIQLDRVFIGSCTNSRIE 359
Query: 309 DLRMAAEILEGQKVAKWVR-LIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG 367
DLR AAE+ G+KVA ++ +V+P S V +A KEGL IF+EAG P C CL
Sbjct: 360 DLRAAAEVARGRKVAATIKQALVVPGSGLVKEQAEKEGLDRIFIEAGFEWREPGCSMCLA 419
Query: 368 GHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEEVV 422
+ L SGE ST+NRNF GR G +L +P +AAA+AV GR E++
Sbjct: 420 MNPDRLESGEHCASTSNRNFEGRQG-AGGRTHLVSPAMAAAAAVNGRFIDVRELL 473
>sp|P48573|LEU2_BUCRP 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|899390|emb|CAA50617.1| (X71612) isopropylmalate
isomerase subunit [Buchnera aphidicola]
Length = 471
Score = 224 bits (566), Expect = 1e-57
Identities = 159/464 (34%), Positives = 242/464 (51%), Gaps = 55/464 (11%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ EKI H EE I+ L + ++VT P A R + + V ++
Sbjct: 1 MKKTLYEKIYDAHIVHEEKNNASILYIDLHLL--HEVTSPQAFDSLR-IKNRVVRQPKKT 57
Query: 61 AIVLDH--FTPNKDIKSAE-----QCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPELGL 112
+DH T +KDI ++ Q + + I + G+ H + PE G+
Sbjct: 58 FATMDHNVSTESKDINASGSMAKIQMQQLIRNCNQFNISLYDLNNPKQGIVHVIGPEQGM 117
Query: 113 VLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPY 172
LPG I+ DSHT T+GA GA + G+G++++ + T + + MK +G+++ +
Sbjct: 118 TLPGMTIVCGDSHTSTHGAFGALSFGIGTSEVEHVLVTQTLKQQRLKNMKIQIKGKIKNF 177
Query: 173 VTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDK 232
VT KD+IL IG +G +G V+EF G VI+++++E+RMT+ NMAIE GAK+ +I PD+
Sbjct: 178 VTAKDIILFIIGKLGTSGGSGYVIEFCGDVIKKMNMEERMTVCNMAIEMGAKSALIAPDE 237
Query: 233 KTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENT 281
T Y+K E A + +K + A + K +DV+N P + + P+
Sbjct: 238 TTFLYLKGRRYSPQGKFWEEAVKYWKTLITRSKAFFDKEFTFDVSNLSPQITWGTSPDQV 297
Query: 282 VPISK----------AAKMN-------------------IKIDQVFIGSCTNGRIEDLRM 312
+ I++ + K N IKID+VFIGSCTN RIEDLR
Sbjct: 298 LSINEKIPDFNFFKDSVKKNLARSACDYMNLKPGSYLTDIKIDKVFIGSCTNSRIEDLRS 357
Query: 313 AAEILEGQKVAKWVRLIVIPCS--PTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHM 370
AA+IL+ +K++K ++ I++P S TV A KEGL +IF++AG P C CLG +
Sbjct: 358 AAKILKNKKISKNIKAILLPGSGTGTVKKPAEKEGLDKIFIDAGFEWRLPGCSMCLGMNN 417
Query: 371 GVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGR 414
L+ GER ST+NRNF GR G +L +P +AAA A+ GR
Sbjct: 418 DKLSDGERCASTSNRNFEGRQGR-GGRTHLVSPIMAAA-AIYGR 459
>gb|AAG31403.1| (AF197456) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 442
Score = 224 bits (564), Expect = 2e-57
Identities = 152/440 (34%), Positives = 228/440 (51%), Gaps = 51/440 (11%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ +KI H EE I+ L + ++VT P A R+ + V ++
Sbjct: 1 MNKTLYQKIYDSHIIHEEKNNTSILYIDLHLL--HEVTSPQAFYSLRQKR-RMVRQPKKT 57
Query: 61 AIVLDH--FTPNKDIKSAE-----QCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPELGL 112
+DH T N+DI + Q + R+ E I + + G+ H + PE G+
Sbjct: 58 FATMDHNVSTHNRDINGSGSMAKIQMEQLRKNCDEFNISLYDINNPNQGIVHVIAPEKGM 117
Query: 113 VLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPY 172
LPG I+ DSHT T+GA GA + G+G++++ +AT + + MK G++ +
Sbjct: 118 TLPGMTIVCGDSHTSTHGAFGALSFGIGTSEVEHVLATQTLKQQRFKNMKIEIIGQIPKF 177
Query: 173 VTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDK 232
++ KD+IL IG G + V+EF G+VIE +S+E+RMT+ NMAIE GAK+G+I PDK
Sbjct: 178 ISAKDIILFIIGKXGSSIGSGYVVEFCGNVIETMSMEERMTICNMAIEMGAKSGLIAPDK 237
Query: 233 KTLDYVKERAKRKFKVY-----------KSDEDAKYYKVIEYDVTNWEPVVAFPHLPENT 281
T Y+K + + V+ KSD+DA + K+ D++N P + + P+
Sbjct: 238 TTYAYLKNKIYSPYGVFWEKSLDFWQTLKSDKDAFFDKIFTIDISNLAPQITWGTNPDQV 297
Query: 282 VPISK-------------------AAKM----------NIKIDQVFIGSCTNGRIEDLRM 312
+ I + A K NI ID+VFIGS TN RIEDLR+
Sbjct: 298 ISIDEKIPNYETFSSLTQRNLAKSACKYMGLKKESYLTNISIDKVFIGSXTNARIEDLRL 357
Query: 313 AAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGV 372
AA+IL+ +K++ V+ IV+P S +V +A +EGL +IF+ AG P C CLG +
Sbjct: 358 AAKILKNKKISNNVQAIVVPGSGSVKRQAEREGLDKIFINAGFEWRLPGCSMCLGMNRDR 417
Query: 373 LASGERAVSTTNRNFVGRMG 392
L GER S +NRNF GR G
Sbjct: 418 LNVGERCASXSNRNFEGRQG 437
>gb|AAG31394.1| (AF197453) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 442
Score = 224 bits (564), Expect = 2e-57
Identities = 151/440 (34%), Positives = 229/440 (51%), Gaps = 51/440 (11%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ +KI H + + I+ L + ++VT P A + R ++V +
Sbjct: 1 MSKTLYQKIYDSHIVYNDQEKTSILYIDLHLL--HEVTSPQAFESLRNKK-RKVRQPNKT 57
Query: 61 AIVLDH--FTPNKDIKSA-----EQCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPELGL 112
+DH T KDI ++ +Q + + E I + G+ H + PE G+
Sbjct: 58 FATMDHNVSTQTKDINASGLMAQKQMEQLIKNCNEFNISLYDINHPHQGIVHVIAPESGM 117
Query: 113 VLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPY 172
LPG I+ DSHT T+GA GA + G+G++++ +AT + + MK GE+ +
Sbjct: 118 TLPGMTIVCGDSHTSTHGAFGALSFGIGTSEVEHVLATQTLKQKRFKNMKIEIIGEIPKF 177
Query: 173 VTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDK 232
VT KD+IL IG +G + V+EF G +I+++S+E+RMT+ NMAIE GAK+G+IEPD+
Sbjct: 178 VTAKDIILFIIGKLGTSSGSGYVVEFCGKIIKKMSMEERMTICNMAIEMGAKSGLIEPDE 237
Query: 233 KTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENT 281
T Y+K E++ + KSD+DA + K D++N P + + P+
Sbjct: 238 ITYAYLKNKIYSPRGLFWEQSLNYWDTLKSDKDAYFDKHFTIDISNLAPQITWGTTPDQV 297
Query: 282 VPISK---------------AAK--------------MNIKIDQVFIGSCTNGRIEDLRM 312
+ I + AAK +I ID+VFIGSCTN RIEDLR
Sbjct: 298 ISIDEKIPNYEEFNCLVKKNAAKSACEYMGLKPDTYLTDIAIDKVFIGSCTNARIEDLRS 357
Query: 313 AAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGV 372
A+ IL+ +KV+ V+ IV+P S V +A KEGL +IF+++G P C CLG +
Sbjct: 358 ASIILKNKKVSNNVKAIVVPGSGLVKTQAEKEGLDKIFIDSGFEWRLPGCSMCLGMNRDR 417
Query: 373 LASGERAVSTTNRNFVGRMG 392
L+ GER S +NRNF GR G
Sbjct: 418 LSFGERCASXSNRNFEGRQG 437
>gb|AAG31382.1| (AF197449) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 442
Score = 223 bits (563), Expect = 3e-57
Identities = 154/440 (35%), Positives = 229/440 (52%), Gaps = 51/440 (11%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ +KI H E+ I+ L + ++VT P A R K R+
Sbjct: 1 MSKTLYQKIYDSHIVYEDKNSESILYIDLHLL--HEVTSPQAFDALRNKQRKVRQSRKTF 58
Query: 61 AIVLDH--FTPNKDIKSA-----EQCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPELGL 112
A +DH T ++I ++ +Q + + +E I + + G+ H + PE G+
Sbjct: 59 A-TMDHNVSTTIQNISASGSMAQKQMEQLIKNCQEFNIPLYDINNPNQGIVHVIAPEKGM 117
Query: 113 VLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPY 172
LPG I+ DSHT T+GA GA A G+G++++ +AT + + MK GE+ +
Sbjct: 118 TLPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLKQKRFKNMKIEIIGEIPKF 177
Query: 173 VTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDK 232
VT KD+IL IG +G + V+EF G VI+ +S+E+RMT+ NMAIE GAK+G+I PD+
Sbjct: 178 VTAKDIILFIIGKLGSSSGTGYVIEFCGDVIKNMSMEERMTICNMAIEMGAKSGLIAPDE 237
Query: 233 KTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENT 281
T Y+K E++ +K KSD++A + K + D++N P + + P+
Sbjct: 238 ITYKYLKNKIYSPSGLSWEKSLDHWKFLKSDKNAYFDKCVTVDISNLAPQITWGTNPDQV 297
Query: 282 VPI------------------SKAA-----------KMNIKIDQVFIGSCTNGRIEDLRM 312
+ I SK+A NI ID+VFIGSCTN RIEDLR
Sbjct: 298 ISIDEKIPDYNNINSAIKRESSKSACEYMGLQSNTYLTNISIDRVFIGSCTNARIEDLRS 357
Query: 313 AAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGV 372
A++IL+ +K+AK V+ IV+P S V +A +EGL IF++AG P C CLG +
Sbjct: 358 ASKILKNKKIAKHVKAIVVPGSKLVKRQAEQEGLDRIFIDAGFEWRLPGCSMCLGMNKDR 417
Query: 373 LASGERAVSTTNRNFVGRMG 392
L G R S +NRNF GR G
Sbjct: 418 LHFGGRCASXSNRNFEGRQG 437
>pir||D36889 3-isopropylmalate dehydratase (EC 4.2.1.33) chain leuC -
Lactococcus lactis subsp. lactis (strain IL1403)
Length = 459
Score = 223 bits (563), Expect = 3e-57
Identities = 157/461 (34%), Positives = 235/461 (50%), Gaps = 46/461 (9%)
Query: 2 GMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIA 61
G TI +K+ H GE + +D ++VT P A + RE G +RV ++
Sbjct: 3 GKTIFDKLWDQHVIAGN--EGEPQLLYIDLHVIHEVTSPQAFQGLREAG-RRVRRKDLTY 59
Query: 62 IVLDHFTPNKDIKSAEQCKSSREF------AKEMGIKWFFEGGS-VGVEHCLLPELGLVL 114
LDH P +DI + + S ++ KE + GG G+ H + PE G
Sbjct: 60 GTLDHNVPTQDIFNIQDLISKKQIDTFTKNVKEFDVPAETHGGKGQGIVHMVAPESGRTQ 119
Query: 115 PGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVT 174
PG I+ DSHT T GA GA A G+G++++ +AT W P+ MK ++G Q +
Sbjct: 120 PGKTIVCGDSHTATNGAFGAIAFGIGTSEVEHVLATQTIWQVKPKRMKIEFQGHPQKGIY 179
Query: 175 GKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKT 234
KD IL I GV+ + +E+SG I +LS+E+RMT+ NM+IE GAK G++ PD+KT
Sbjct: 180 SKDFILALIAKYGVDAGVGYAVEYSGDAISDLSMEERMTICNMSIEFGAKIGLMNPDEKT 239
Query: 235 LDYVKER---------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVA------------ 273
DYVK R A K++ SD DA+Y K++ DV+ +P+V
Sbjct: 240 YDYVKGREHAPKNFDEAVSKWEKLVSDSDAQYDKILSLDVSQLKPMVTWGTNPGMGLEFG 299
Query: 274 --FPHLPENTVPISKAAK-MNIKIDQ---------VFIGSCTNGRIEDLRMAAEILEGQK 321
FP + N + +A + M++K Q +FIGSCTN R+ DL AA+I+ +
Sbjct: 300 EKFPEI-NNDLNYERAYQYMDLKPGQTASAIDLGYIFIGSCTNARLGDLEEAAKIIGDRH 358
Query: 322 VAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGERAVS 381
+A + IV+P S V A +GL +IF +AG P C CLG + + S
Sbjct: 359 IADGLTGIVVPGSRPVKEAAEAQGLDKIF-KAGFEWREPGCSACLGMNPDQIPEYVHCAS 417
Query: 382 TTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEEVV 422
T+NRNF GR GH + ++ +P +AAA+A+ G+ +V
Sbjct: 418 TSNRNFEGRQGH-NARTHVCSPAMAAAAAIAGKFVDVRMLV 457
>gb|AAG31385.1| (AF197450) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 443
Score = 222 bits (561), Expect = 4e-57
Identities = 149/440 (33%), Positives = 230/440 (51%), Gaps = 51/440 (11%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ +KI H E+ I+ L + ++VT P A R ++V +
Sbjct: 1 MSKTLYQKIYDSHVVCEDKNNTSILYIDLHLL--HEVTSPQAFDSLRNKK-RKVRQVNKT 57
Query: 61 AIVLDH--FTPNKDIKSA-----EQCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPELGL 112
+DH T K+I ++ +Q + + +E I + + G+ H + PE G+
Sbjct: 58 FATMDHNVSTQIKNINASGSMAKKQMEQLIKNCQEFNISLYDINNPNQGIVHVIAPEKGM 117
Query: 113 VLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPY 172
LPG I+ DSHT T+GA GA + G+G++++ +AT + + MK GE+ +
Sbjct: 118 TLPGMTIVCGDSHTSTHGAFGALSFGIGTSEVEHVLATQTLKQKRFKNMKIEIVGEIPKF 177
Query: 173 VTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDK 232
VT KD+IL IG +G + V+EF G+V++++S+E+RMT+ NMAIE GAK+G+I PD+
Sbjct: 178 VTAKDIILFIIGKLGSSSGTGYVIEFCGNVVKKMSMEERMTVCNMAIEMGAKSGLIAPDE 237
Query: 233 KTLDYVKERAKRKFKVY-----------KSDEDAKYYKVIEYDVTNWEPVVAFPHLPENT 281
T +Y+K + + ++ KSD DA++ K D++N P + + P+
Sbjct: 238 ITYEYLKNKMYSPYGIFWKNSLDYWRFLKSDSDAQFDKYFTIDISNLAPQITWGTNPDQV 297
Query: 282 VPISK---------------AAK--------------MNIKIDQVFIGSCTNGRIEDLRM 312
+ I++ AAK NI ID+VFIGSCTN RIEDLR
Sbjct: 298 ISINEKIPDYNKIDSLVKKDAAKSACKYMGLRSDTYLTNISIDRVFIGSCTNARIEDLRS 357
Query: 313 AAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGV 372
A+ IL+ +K+A V+ IV+P S V +A KEGL +IF+++G P C CLG +
Sbjct: 358 ASVILKNKKIANHVKAIVVPGSGLVKKQAEKEGLDKIFIDSGFEWRLPGCSMCLGMNKDR 417
Query: 373 LASGERAVSTTNRNFVGRMG 392
L ER S +NRNF GR G
Sbjct: 418 LNFQERCASHSNRNFEGRQG 437
>pir||H82564 3-isopropylmalate dehydratase large subunit XF2375 [imported] -
Xylella fastidiosa (strain 9a5c)
>gi|9107553|gb|AAF85174.1|AE004047_5 (AE004047)
3-isopropylmalate dehydratase large subunit [Xylella
fastidiosa]
Length = 474
Score = 217 bits (548), Expect = 1e-55
Identities = 141/364 (38%), Positives = 197/364 (53%), Gaps = 42/364 (11%)
Query: 101 GVEHCLLPELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPET 160
G+ H + PE G LPG ++ DSHT T+GA GA A G+G++++ +AT + +
Sbjct: 112 GIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLVTQKMKN 171
Query: 161 MKFIYEGELQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIE 220
M+ EG L VT KD++L IG IG G +EFSGS I LS+E RMT+ NMAIE
Sbjct: 172 MQVRVEGTLPWGVTAKDIVLALIGKIGTAGGNGYAVEFSGSTIRALSMEGRMTICNMAIE 231
Query: 221 AGAKTGIIEPDKKTLDYVKER-----------AKRKFKVYKSDEDAKYYKVIEYDVTNWE 269
AGA+ G++ D+KT+ YV R A ++ SD DA + +V+E +
Sbjct: 232 AGARVGMVAVDEKTIQYVHGRPFAPKGSDWDAAVAFWRGLVSDPDAHFDRVVELSAEEIK 291
Query: 270 PVVAFPHLPE--------------NTVPISKAAKM---------------NIKIDQVFIG 300
P V + PE T P+ K + + +IK+D+VFIG
Sbjct: 292 PQVTWGTSPEMVSAVDQSVPDPERETDPVKKESLIRALKYMGLQPNDPITSIKLDRVFIG 351
Query: 301 SCTNGRIEDLRMAAEILEGQKVAKWVR-LIVIPCSPTVYWKALKEGLIEIFLEAGAVIGP 359
SCTN RIEDLR AAE+++G+KVA V+ +V+P S V +A EGL +IF+EAG
Sbjct: 352 SCTNSRIEDLRAAAEVVKGRKVASTVKQAMVVPGSGLVKAQAEVEGLDKIFIEAGFEWRE 411
Query: 360 PTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPE 419
P C CL + L SGE ST+NRNF GR G +L +P +AAA+AV G
Sbjct: 412 PGCSMCLAMNPDKLGSGEHCASTSNRNFEGRQG-IGGRTHLVSPAMAAAAAVAGHFVDVR 470
Query: 420 EVVK 423
E+++
Sbjct: 471 EMMR 474
>gb|AAG31379.1| (AF197448) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 443
Score = 217 bits (547), Expect = 2e-55
Identities = 147/440 (33%), Positives = 220/440 (49%), Gaps = 51/440 (11%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ +KI H E+ I+ L + ++VT P A R + V ++
Sbjct: 1 MSKTLYQKIYDSHVVYEDKNSTSILYIDLHLL--HEVTSPQAFDSLRNKQ-RTVRQSKKT 57
Query: 61 AIVLDHFTPNK-------DIKSAEQCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPELGL 112
+DH K + +Q + + +E I + G+ H + PE G+
Sbjct: 58 FATMDHNVSTKIQNISASGSMAQKQMEQLIQNCQEFNIPLYDINNPHQGIVHVIAPEKGM 117
Query: 113 VLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPY 172
LPG I+ DSHT T+GA GA A G+G++++ +AT + + MK G++ +
Sbjct: 118 TLPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLKQKRFKNMKIEIIGKIPRF 177
Query: 173 VTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDK 232
VT KD+IL IG +G + V+EF G VI +S+E+RMT+ NMAIE GAK+G+I PD+
Sbjct: 178 VTAKDIILFIIGKLGSSSGTGHVIEFCGDVIRNMSMEERMTICNMAIEMGAKSGLIAPDE 237
Query: 233 KTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENT 281
T Y+K +++ +K+ KSD +A Y K +++N P + + +
Sbjct: 238 ITYSYLKNKIYSPSGLFWQKSLDYWKLLKSDHNAHYDKCFTVNISNLAPQITWGTNLDQV 297
Query: 282 VPI-----------------------------SKAAKMNIKIDQVFIGSCTNGRIEDLRM 312
+ I S NI ID+VFIGSCTN RIEDLR
Sbjct: 298 ISIDEKIPDFNDVNSTIKKDSAKSACEYMGLKSNTYLTNISIDKVFIGSCTNARIEDLRS 357
Query: 313 AAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGV 372
AA IL+ +K+AK V+ IV+P S +V +A +EGL +IF+++G P C CLG +
Sbjct: 358 AAGILKNKKIAKHVQAIVVPGSGSVKRQAEREGLDKIFIDSGFEWRLPGCSMCLGMNKDR 417
Query: 373 LASGERAVSTTNRNFVGRMG 392
L GER S +NRNF GR G
Sbjct: 418 LNFGERCASXSNRNFEGRQG 437
>gb|AAG31391.1| (AF197452) isopropylmaltate dehydratase subunit [Buchnera
aphidicola]
Length = 443
Score = 217 bits (546), Expect = 3e-55
Identities = 144/440 (32%), Positives = 227/440 (50%), Gaps = 51/440 (11%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ +KI H ++ I+ L + ++VT P A R + V ++
Sbjct: 1 MSKTLYQKIYDSHVVYKDKSNMSILYIDLHLI--HEVTSPQAFDSLRHKK-RGVRQPQKT 57
Query: 61 AIVLDH--FTPNKDIKSA-----EQCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPELGL 112
+DH T +K+I ++ +Q + + E I + + G+ H + PE G+
Sbjct: 58 FATMDHNVSTQSKNINASGSMAQKQMEQLIKNCNEFNISLYDVNNPNQGIVHVIAPEKGM 117
Query: 113 VLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPY 172
LPG I+ DSHT T+GA GA + G+G++++ +AT + + MK G++ +
Sbjct: 118 TLPGMTIVCGDSHTSTHGAFGALSFGIGTSEVEHVLATQTLKQKRFKNMKITIIGKVPEF 177
Query: 173 VTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDK 232
+T KD+IL IG +G + V+EF G VI +S+E+RMT+ NMAIE GAK+G+I PD+
Sbjct: 178 ITAKDIILFIIGQLGSSRGTGYVIEFFGDVINNMSMEERMTLCNMAIEMGAKSGLIAPDE 237
Query: 233 KTLDYVKERAKRKFKVY-----------KSDEDAKYYKVIEYDVTNWEPVVAFPHLPENT 281
T Y+K + + ++ KSD++A + K D++N P + + P+
Sbjct: 238 ITYSYLKNKIYSPYGIFWKKAIDYWSTLKSDKNAYFDKFFTIDISNLAPQITWGTNPDQV 297
Query: 282 VPISK-------------------AAK----------MNIKIDQVFIGSCTNGRIEDLRM 312
+ I + A K ++ +D+VFIGSCTN RIEDLR
Sbjct: 298 ISIDEKIPNHNKFDSSIIRDSIKSACKYMGLKEGTYLTDVSVDKVFIGSCTNARIEDLRS 357
Query: 313 AAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGV 372
A++IL+ +K++K V+ IV+P S +V +A KEGL +IF+ AG P C CLG +
Sbjct: 358 ASKILKNKKISKNVKAIVVPGSGSVKEQAEKEGLDKIFINAGFEWRLPGCSMCLGMNNDR 417
Query: 373 LASGERAVSTTNRNFVGRMG 392
L GER S +NRNF GR G
Sbjct: 418 LNFGERCASXSNRNFEGRQG 437
>gb|AAG31929.1|AF200469_3 (AF200469) isopropylmalate dehydratase subunit [Buchnera
aphidicola]
Length = 444
Score = 210 bits (529), Expect = 3e-53
Identities = 146/437 (33%), Positives = 224/437 (50%), Gaps = 51/437 (11%)
Query: 4 TIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIAIV 63
T+ +KI H ++ G I+ L + ++VT P A R + V ++
Sbjct: 5 TLYQKIYDSHIVYQDKNGGAILYIDLHLL--HEVTSPQAFDALRSKK-REVRQSKKTFAT 61
Query: 64 LDH--FTPNKDIKSA-----EQCKSSREFAKEMGIKWF-FEGGSVGVEHCLLPELGLVLP 115
+DH T ++I ++ +Q + + +E I + + G+ H + PE G+ LP
Sbjct: 62 MDHNVSTTIQNISASGSMARKQMEQLIKNCQEFNIPLYDINNPNQGIVHVIAPEKGMTLP 121
Query: 116 GDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTG 175
G I+ DSHT T+GA GA A G+G++++ +AT + + MK G++ +VT
Sbjct: 122 GMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLKQKRFKNMKIEIIGKIPKFVTA 181
Query: 176 KDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTL 235
KD+IL IG +G + V+EF G++I+ +S+E+RMT+ NMAIE AK+G+I P T
Sbjct: 182 KDIILFVIGQLGSSSGTGYVIEFCGNIIKNMSMEERMTVCNMAIEMXAKSGLIAPXXITY 241
Query: 236 DYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPI 284
Y+K E++ +K KSD++A + K I D++N P + + P+ + I
Sbjct: 242 KYLKDKIYSPSGLFWEKSLDYWKCLKSDKNAYFDKCITVDISNLAPQITWGTNPDQVISI 301
Query: 285 -----------------------------SKAAKMNIKIDQVFIGSCTNGRIEDLRMAAE 315
S NI ID+VFIGS TN RIEDLR AA+
Sbjct: 302 DEKIPDYNNINSEVKKDSAKSACKYMGLKSNTYLTNISIDKVFIGSXTNARIEDLRSAAK 361
Query: 316 ILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLAS 375
IL+ +K+AK V+ IV+P S V KA +EGL +IF+++G P C LG + L
Sbjct: 362 ILQNRKIAKHVKAIVVPGSGLVKKKAEQEGLDKIFIDSGFEWRLPGCSMXLGMNKDRLNF 421
Query: 376 GERAVSTTNRNFVGRMG 392
GER S +NRNF GR G
Sbjct: 422 GERXXSHSNRNFEGRQG 438
>sp|P15717|LEU2_SALTY 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
Length = 465
Score = 210 bits (528), Expect = 3e-53
Identities = 158/467 (33%), Positives = 222/467 (46%), Gaps = 59/467 (12%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ EK+ H E P E + +D ++VT P A R V +
Sbjct: 1 MAKTLYEKLFDAHVVFEA--PNETPLLYIDRHLVHEVTSPQAFDGLRA-HPSPVRQPGKT 57
Query: 61 AIVLDH--FTPNKDIKSA---------EQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPE 109
+DH T KDI ++ E K+ EF E+ + G+ H + PE
Sbjct: 58 FATMDHNVSTQTKDINASGEMARIQMQELIKNCNEFGVEL---YDLNHPYQGIVHVMGPE 114
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGEL 169
G+ LPG I+ DSHT T+GA GA A G+G++++ +AT +TMK G
Sbjct: 115 QGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVTGNA 174
Query: 170 QPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIE 229
P +T KD++L IG G G V+EF G I LS+E RMT+ NMAIE GAK G++
Sbjct: 175 APGITAKDIVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVA 234
Query: 230 PDKKTLDYVKER-----------AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLP 278
PD+ T +YVK R A +K K+D+ A + V+ P V + P
Sbjct: 235 PDETTFNYVKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVALRAEEIAPQVTWGTNP 294
Query: 279 ENTV--------------PISKAAK---------------MNIKIDQVFIGSCTNGRIED 309
+ P+ +A+ ++ ID+VFIG CTN RIED
Sbjct: 295 GQVISVTDIIPDPASFSDPVERASAEKALAYMGLQPGVPLTDVAIDKVFIGPCTNSRIED 354
Query: 310 LRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGH 369
LR AE+ +G K+ V+ +V+P S V +A EGL +IF+EAG P C C+ +
Sbjct: 355 LRAPAEVGKGPKLPG-VQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCVAVN 413
Query: 370 MGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIA 416
L GER ST+NRNF GR G +L +P +AAA+AV G A
Sbjct: 414 NDRLNPGERCASTSNRNFEGRQGR-GGRTHLVSPAMAAAAAVTGHFA 459
>sp|P54384|LEU2_SYNY3 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|7450354|pir||S76826 hypothetical protein -
Synechocystis sp. (strain PCC 6803)
>gi|1653827|dbj|BAA18738.1| (D90916) 3-isopropylmalate
dehydratase [Synechocystis sp.]
Length = 468
Score = 207 bits (522), Expect = 2e-52
Identities = 138/435 (31%), Positives = 208/435 (47%), Gaps = 48/435 (11%)
Query: 35 NDVTMPLAIKKFRELGVKRVFDRERIAIVLDHFTPNK-------DIKSAEQCKSSREFAK 87
++VT P A +E G+ RV ER +DH P + D + E ++ K
Sbjct: 34 HEVTSPQAFAMLKERGL-RVMYPERTVATVDHIVPTENQQRPFQDSLAEEMMQALEVNTK 92
Query: 88 EMGIKWFFEG-GSVGVEHCLLPELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAV 146
I++ G G+ G+ H + PE GL PG I DSHT T+GA GA A G+G++ +
Sbjct: 93 AHNIRFHPIGSGNQGIVHVIAPEQGLTQPGMTIACGDSHTSTHGAFGAIAFGIGTSQVRD 152
Query: 147 AMATGEAWFRVPETMKFIYEGELQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEEL 206
+A + K G+L P V KD+ILH I +GV G + E++GS +
Sbjct: 153 VLAAQSLSLNKLKVRKIEVNGDLAPGVYAKDVILHIIRKLGVKGGVGYAYEYAGSTFVAM 212
Query: 207 SVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVK-----------ERAKRKFKVYKSDEDA 255
++E+RMT+ NM+IE GA+ G I PD+ T Y+K ++A + ++ S+ DA
Sbjct: 213 NMEERMTVCNMSIEGGARCGYINPDEVTFAYLKGREFAPQGEDWDKAVQWWRSIASEPDA 272
Query: 256 KYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAK-------------------------- 289
Y V+ + + EP V + P + IS+
Sbjct: 273 VYDDVVSFRAEDIEPTVTWGITPGQGIGISEPVPTLDSLNPDDQAIASEAYKYMQFQPGQ 332
Query: 290 --MNIKIDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLI 347
K+D FIGSCTNGR+ DL AA++ +G +VA V+ V+P S V +A GL
Sbjct: 333 PLKGTKVDVCFIGSCTNGRLSDLEEAAKVAKGHQVAAGVKAFVVPGSEQVKQQAEAAGLD 392
Query: 348 EIFLEAGAVIGPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAA 407
+IF AG C CL + L + + S++NRNF GR G L +P + A
Sbjct: 393 QIFQAAGFEWREAGCSMCLAMNPDKLQGDQLSASSSNRNFKGRQGSASGRTLLMSPAMVA 452
Query: 408 ASAVLGRIASPEEVV 422
A+A+ G++A EV+
Sbjct: 453 AAAITGQVADVREVL 467
>pir||S10171 3-isopropylmalate dehydratase (EC 4.2.1.33) chain leuC - Salmonella
typhimurium >gi|47762|emb|CAA35840.1| (X51476) leuC gene
product (AA 1-464) [Salmonella typhimurium]
>gi|154158|gb|AAA27155.1| (M31047) isopropylmalate
synthase (EC 4.2.1.3) [Salmonella typhimurium]
Length = 464
Score = 187 bits (471), Expect = 2e-46
Identities = 134/384 (34%), Positives = 184/384 (47%), Gaps = 45/384 (11%)
Query: 73 IKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLIIGADSHTCTYGAL 132
I+ E K+ EF E+ + G+ H + PE G+ LPG I+ DSHT T+GA
Sbjct: 80 IQMQELIKNCNEFGVEL---YDLNHPYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAF 136
Query: 133 GAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLILHTIGDIGVNGAL 192
GA A G+G++++ +AT +TMK G P +T KD++L IG G G
Sbjct: 137 GALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVTGNAAPGITAKDIVLAIIGKTGSAGGT 196
Query: 193 YKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKER----------- 241
V+EF G I LS+E RMT+ NMAIE GAK G++ PD+ T +YVK R
Sbjct: 197 GHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGRDFDE 256
Query: 242 AKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTV--------------PISKA 287
A +K K+D+ A + V+ P V + P + P+ +A
Sbjct: 257 AVEYWKTLKTDDGATFDTVVALRAEEIAPQVTWGTNPGQVISVTDIIPDPASFSDPVERA 316
Query: 288 AK---------------MNIKIDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVRLIVIP 332
+ ++ ID+VFIG CTN RIEDLR AE+ + KVA
Sbjct: 317 SAEKALAYMGLQPGVPLTDVAIDKVFIGPCTNSRIEDLRAPAEVGQRAKVAGRAGAGGAG 376
Query: 333 CSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMG 392
A EGL +IF+EAG P C C+ + L GER ST+NRNF GR G
Sbjct: 377 FRSGES-AAEAEGLDKIFIEAGFEWRLPGCSMCVAVNNDRLNPGERCASTSNRNFEGRQG 435
Query: 393 HPKSEVYLANPYVAAASAVLGRIA 416
+L +P +AAA+AV G A
Sbjct: 436 R-GGRTHLVSPAMAAAAAVTGHFA 458
>gb|AAA34389.1| (M33131) aconitase (ACO1) (EC 4.2.1.3) [Saccharomyces cerevisiae]
Length = 779
Score = 164 bits (410), Expect = 2e-39
Identities = 132/446 (29%), Positives = 213/446 (47%), Gaps = 41/446 (9%)
Query: 4 TIAEKILADHSER---EEVKPG-EIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRER 59
T AEKIL H + ++++ G + + D V D T +AI +F G+ +V +
Sbjct: 60 TYAEKILYGHLDDPHGQDIQRGVSYLKLRPDRVACQDATAQMAILQFMSAGLPQV--AKP 117
Query: 60 IAIVLDHFTP-----NKDIKSA-EQCKSSREFAKEMGIKW---FFEGGSVGVEHCLLPEL 110
+ + DH KD+K A + K +F K+ F++ GS G+ H ++ E
Sbjct: 118 VTVHCDHLIQAQVGGEKDLKRAIDLNKEVYDFLASATAKYNMGFWKPGS-GIIHQIVLE- 175
Query: 111 GLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGEL 169
PG LIIG DSHT G LG A GVG D AV + G W + P+ + G++
Sbjct: 176 NYAFPGALIIGTDSHTPNAGGLGQLAIGVGGAD-AVDVMAGRPWELKAPKILGVKLTGKM 234
Query: 170 QPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIE 229
+ + KD+IL G V G K++E+ G ++ S T+ NM E GA T +
Sbjct: 235 NGWTSPKDIILKLAGITTVKGGTGKIVEYFGDGVDTFSATGMGTICNMGAEIGATTSVFP 294
Query: 230 PDKKTLDYVKERAKRKF----KVY-----KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+K ++Y++ + K K+Y +D+DA+Y +V+E D+ EP + P P+
Sbjct: 295 FNKSMIEYLEATGRGKIADFAKLYHKDLLSADKDAEYDEVVEIDLNTLEPYINGPFTPDL 354
Query: 281 TVPISK------AAKMNIKIDQVFIGSCTNGRIEDLRMAAEILE---GQKVAKWVRLIVI 331
P+SK A + + IGSCTN ED+ +A I++ + V
Sbjct: 355 ATPVSKMKEVAVANNWPLDVRVGLIGSCTNSSYEDMSRSASIVKDAAAHGLKSKTIFTVT 414
Query: 332 PCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNFV 388
P S + ++G +E F E G ++ CGPC+G + G++ VS+ NRNF
Sbjct: 415 PGSEQIRATIERDGQLETFKEFGGIVLANACGPCIGQWDRRDIKKGDKNTIVSSYNRNFT 474
Query: 389 GRM-GHPKSEVYLANPYVAAASAVLG 413
R G+P++ ++A+P + A A+ G
Sbjct: 475 SRNDGNPQTHAFVASPELVTAFAIAG 500
>gi|6323335 Aconitase, mitochondrial; Aco1p [Saccharomyces cerevisiae]
>gi|1168308|sp|P19414|ACON_YEAST ACONITATE HYDRATASE,
MITOCHONDRIAL PRECURSOR (CITRATE HYDRO-LYASE)
(ACONITASE) >gi|1077349|pir||S50387 aconitate hydratase
(EC 4.2.1.3) precursor - yeast (Saccharomyces
cerevisiae) >gi|596052|gb|AAB67348.1| (U17243) Aconitate
hydratase [Saccharomyces cerevisiae]
Length = 778
Score = 164 bits (410), Expect = 2e-39
Identities = 132/446 (29%), Positives = 213/446 (47%), Gaps = 41/446 (9%)
Query: 4 TIAEKILADHSER---EEVKPG-EIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRER 59
T AEKIL H + ++++ G + + D V D T +AI +F G+ +V +
Sbjct: 60 TYAEKILYGHLDDPHGQDIQRGVSYLKLRPDRVACQDATAQMAILQFMSAGLPQV--AKP 117
Query: 60 IAIVLDHFTP-----NKDIKSA-EQCKSSREFAKEMGIKW---FFEGGSVGVEHCLLPEL 110
+ + DH KD+K A + K +F K+ F++ GS G+ H ++ E
Sbjct: 118 VTVHCDHLIQAQVGGEKDLKRAIDLNKEVYDFLASATAKYNMGFWKPGS-GIIHQIVLE- 175
Query: 111 GLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGEL 169
PG LIIG DSHT G LG A GVG D AV + G W + P+ + G++
Sbjct: 176 NYAFPGALIIGTDSHTPNAGGLGQLAIGVGGAD-AVDVMAGRPWELKAPKILGVKLTGKM 234
Query: 170 QPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIE 229
+ + KD+IL G V G K++E+ G ++ S T+ NM E GA T +
Sbjct: 235 NGWTSPKDIILKLAGITTVKGGTGKIVEYFGDGVDTFSATGMGTICNMGAEIGATTSVFP 294
Query: 230 PDKKTLDYVKERAKRKF----KVY-----KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+K ++Y++ + K K+Y +D+DA+Y +V+E D+ EP + P P+
Sbjct: 295 FNKSMIEYLEATGRGKIADFAKLYHKDLLSADKDAEYDEVVEIDLNTLEPYINGPFTPDL 354
Query: 281 TVPISK------AAKMNIKIDQVFIGSCTNGRIEDLRMAAEILE---GQKVAKWVRLIVI 331
P+SK A + + IGSCTN ED+ +A I++ + V
Sbjct: 355 ATPVSKMKEVAVANNWPLDVRVGLIGSCTNSSYEDMSRSASIVKDAAAHGLKSKTIFTVT 414
Query: 332 PCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNFV 388
P S + ++G +E F E G ++ CGPC+G + G++ VS+ NRNF
Sbjct: 415 PGSEQIRATIERDGQLETFKEFGGIVLANACGPCIGQWDRRDIKKGDKNTIVSSYNRNFT 474
Query: 389 GRM-GHPKSEVYLANPYVAAASAVLG 413
R G+P++ ++A+P + A A+ G
Sbjct: 475 SRNDGNPQTHAFVASPELVTAFAIAG 500
>gb|AAB41277.1| (U32936) 3-isopropylmalate dehydratase [Synechocystis PCC6803]
Length = 373
Score = 157 bits (393), Expect = 2e-37
Identities = 106/340 (31%), Positives = 161/340 (47%), Gaps = 48/340 (14%)
Query: 35 NDVTMPLAIKKFRELGVKRVFDRERIAIVLDHFTPNK-------DIKSAEQCKSSREFAK 87
++VT P A +E G+ RV ER +DH P + D + E ++ K
Sbjct: 34 HEVTSPQAFAMLKERGL-RVMYPERTVATVDHIVPTENQQRPFQDSLAEEMMQALEVNTK 92
Query: 88 EMGIKWFFEG-GSVGVEHCLLPELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAV 146
I++ G G+ G+ H + PE GL PG I DSHT T+GA GA A G+G++ +
Sbjct: 93 AHNIRFHPIGSGNQGIVHVIAPEQGLTQPGMTIACGDSHTSTHGAFGAIAFGIGTSQVRD 152
Query: 147 AMATGEAWFRVPETMKFIYEGELQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEEL 206
+A + K G+L P V KD+ILH I +GV G + E++GS +
Sbjct: 153 VLAAQSLSLNKLKVRKIEVNGDLAPGVYAKDVILHIIRKLGVKGGVGYAYEYAGSTFVAM 212
Query: 207 SVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVK-----------ERAKRKFKVYKSDEDA 255
++E+RMT+ NM+IE GA+ G I PD+ T Y+K ++A + ++ S+ DA
Sbjct: 213 NMEERMTVCNMSIEGGARCGYINPDEVTFAYLKGREFAPQGEDWDKAVQWWRSIASEPDA 272
Query: 256 KYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAKM------------------------- 290
Y V+ + + EP V + P + IS+
Sbjct: 273 VYDDVVSFRAEDIEPTVTWGITPGQGIGISEPVPTLDSLNPDDQAIASEAYKYMQFQPGQ 332
Query: 291 ---NIKIDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR 327
K+D FIGSCTNGR+ DL AA++ +G +VA V+
Sbjct: 333 PLKGTKVDVCFIGSCTNGRLSDLEEAAKVAKGHQVAAGVK 372
>gb|AAF54588.1| (AE003690) CG4706 gene product [Drosophila melanogaster]
Length = 769
Score = 155 bits (389), Expect = 7e-37
Identities = 127/447 (28%), Positives = 211/447 (46%), Gaps = 38/447 (8%)
Query: 3 MTIAEKILADHSER---EEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EK+L H ++ +E++ G+ + + D V D T + + +F G+K+V
Sbjct: 47 LTLSEKVLYSHLDQPDSQEIERGKSYLRLRPDRVALQDATAQMTLLQFISSGLKKVAVPS 106
Query: 59 RIA---IVLDHFTPNKDIKSAEQC-KSSREFAKEMGIKW---FFEGGSVGVEHCLLPELG 111
+ ++ + +KD+ A+ K +F K+ F++ GS G+ H ++ E
Sbjct: 107 TVHCDHLIEAQISGDKDLARAKDLNKEVYDFLSSACAKYNLGFWKPGS-GIIHQIILE-N 164
Query: 112 LVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQP 171
PG L+IG DSHT G LG GVG D MA + P + G++
Sbjct: 165 YAFPGLLMIGTDSHTPNGGGLGCLCVGVGGADAVDVMANIPWELKCPTVIGCHLTGKISG 224
Query: 172 YVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPD 231
+ + KD+IL + V G ++E+ G +E +S T++NM E GA T I +
Sbjct: 225 WTSPKDVILKVAEILTVKGGTGAIVEYHGPGVESISCTGMATITNMGAEIGATTSIFPFN 284
Query: 232 KKTLDYVK--------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVP 283
++ + Y++ + A + + DE KY KVIE ++ EP+V P P+ P
Sbjct: 285 ERMVTYLRATGRGAIADEATKNKDLLVPDEGCKYDKVIEINLDTLEPLVNGPFTPDLAHP 344
Query: 284 ISKAAK--------MNIKIDQVFIGSCTNGRIEDLRMAAEILE---GQKVAKWVRLIVIP 332
ISK + M IK+ IGSCTN ED+ A I G + V V P
Sbjct: 345 ISKLGQNSEKNGYPMEIKVS--LIGSCTNSSYEDMGRCASIANDALGHGLKSCVPFNVTP 402
Query: 333 CSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNFVG 389
S V ++G+I++ + G + CGPC+G + GE+ V++ NRNF G
Sbjct: 403 GSEQVRATIARDGIIDVLEKFGGTVLANACGPCIGQWDRKDVKMGEKNTIVTSYNRNFTG 462
Query: 390 RM-GHPKSEVYLANPYVAAASAVLGRI 415
R +P + ++ +P +A A A+ GR+
Sbjct: 463 RNDANPATHCFVTSPEMATALAIAGRL 489
>sp|P49609|ACON_GRAVE ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSOR (CITRATE HYDRO-LYASE)
(ACONITASE) >gi|1361942|pir||S57805 aconitate hydratase
(EC 4.2.1.3) precursor - red alga (Gracilaria
verrucosa) >gi|600171|gb|AAA80494.1| (U17709) aconitase
[Gracilaria gracilis]
Length = 779
Score = 150 bits (375), Expect = 3e-35
Identities = 126/446 (28%), Positives = 214/446 (47%), Gaps = 37/446 (8%)
Query: 3 MTIAEKILADH---SEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRER 59
+T+AEK+L H ER V+ + + + V D T +A+ +F +V
Sbjct: 62 LTLAEKVLYGHLDDPERVPVRGETFLKLRPERVALQDATAQMALIQFMASARPQVAVPST 121
Query: 60 IA---IVLDHFTPNKDI-KSAEQCKSSREFAKEMGIKW---FFEGGSVGVEHCLLPELGL 112
I ++ +D+ K+ Q K +F G K+ F++ GS G+ H ++ E
Sbjct: 122 IHCDHLIAAEVGAEEDMAKAKSQNKEVYDFLASAGAKYGLGFWKPGS-GIIHQIVLE-NY 179
Query: 113 VLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGELQP 171
PG L+IG DSHT G LGA A GVG D AV + G W + P+ + G+LQ
Sbjct: 180 AFPGLLMIGTDSHTPNAGGLGACAVGVGGAD-AVDVMVGLPWELKAPKVIGVKLTGKLQE 238
Query: 172 YVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPD 231
+ + KD+IL G + V G ++E+ G ++ LS T+ NM E GA T + +
Sbjct: 239 WASPKDVILKVAGILTVKGGTGAIVEYFGEGVDSLSCTGMGTICNMGAEIGATTSMFPYN 298
Query: 232 KKTLDYVKERAKRKF--------KVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVP 283
+ DY+K + + ++DE+A Y ++IE +++ EP + P P+ P
Sbjct: 299 SRMGDYLKATGRDGIASLADSFSEQLRADENAVYDQLIEINLSELEPHINGPFTPDLAHP 358
Query: 284 ISKAAK------MNIKIDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIVIPC 333
+SK + ++ IGSCTN ED+ +A +++ Q ++ V+ + P
Sbjct: 359 LSKFKEEVEKNGWPAELTVGLIGSCTNSSYEDMARSASVVK-QALSHGVKSKSIFNITPG 417
Query: 334 SPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG--GHMGVLASGERAVSTT-NRNFVGR 390
S V ++G+++ F EAG + CGPC+G V ++ T+ NRNF R
Sbjct: 418 SEQVRATISRDGILDTFTEAGGTVLANACGPCIGQWNRSDVPKGTPNSIITSFNRNFSQR 477
Query: 391 M-GHPKSEVYLANPYVAAASAVLGRI 415
G+P++ ++A+P + A ++ G +
Sbjct: 478 NDGNPQTHAFVASPEIVTAMSLAGSL 503
>emb|CAB83173.1| (AL162692) aconitate hydratase [Schizosaccharomyces pombe]
Length = 905
Score = 149 bits (373), Expect = 5e-35
Identities = 130/449 (28%), Positives = 202/449 (44%), Gaps = 46/449 (10%)
Query: 3 MTIAEKILADHSEREE-----VKPGEI---VMAKL--DFVFGNDVTMPLAIKKFRELGVK 52
+T+AEK+L H E V P +I + KL D V D + +A+ +F G++
Sbjct: 57 LTLAEKVLYSHLVNPEESFSGVSPSDIRGSLYLKLNPDRVAMQDASAQMALLQFMTCGLE 116
Query: 53 RVFDRERIA---IVLDHFTPNKDIK-SAEQCKSSREF----AKEMGIKWFFEGGSVGVEH 104
+ I +++ H N DI S K +F AK+ GI+ F G G+ H
Sbjct: 117 KTMIPASIHCDHLIVGHRGANSDIPDSIANNKEIFDFLQSAAKKYGIQ--FWGPGSGIIH 174
Query: 105 CLLPELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFI 164
++ E PG +++G DSHT G LG A GVG D AM + P+ +
Sbjct: 175 QIVLE-NYAAPGGMMLGTDSHTPNAGGLGMIAIGVGGADAVDAMTNTPWELKAPKIIGVN 233
Query: 165 YEGELQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAK 224
G + + T KDLILH G + V G ++E+ G + LS T+ NM E GA
Sbjct: 234 LTGAMSGWTTPKDLILHLAGKLTVRGGTGHIIEYFGPGVASLSCTGMATVCNMGAEVGAT 293
Query: 225 TGI-----------IEPDKKTLDYVKERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVA 273
T I I + + ++ +D AKY ++I+ +++ P +
Sbjct: 294 TSIFPYTDSMRRYLIATHRAEVADAASEVHSEYNYLAADTGAKYDQIIDINLSELTPSLN 353
Query: 274 FPHLPENTVPISKAA------KMNIKIDQVFIGSCTNGRIEDLRMAAEILE---GQKVAK 324
P P+ + P+SK K K+ IGSCTN +D+ +++E +
Sbjct: 354 GPFTPDLSTPVSKFGEAIEKNKWPKKLSAGLIGSCTNSSYQDMTCVVDVVEQAISAGLKP 413
Query: 325 WVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG--GHMGVLASGE-RAVS 381
V +V P S + ++G+ E EAGA + CGPC+G +ASGE A+
Sbjct: 414 KVPFLVTPGSEQIRATIERDGITERLEEAGATVLANACGPCIGMWKRTDDIASGEPNAIL 473
Query: 382 TT-NRNFVGRM-GHPKSEVYLANPYVAAA 408
T+ NRNF R G+P + +L +P + AA
Sbjct: 474 TSFNRNFRSRNDGNPSTMNFLTSPVIVAA 502
>sp|O13966|ACON_SCHPO ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSOR (CITRATE HYDRO-LYASE)
(ACONITASE) >gi|7490068|pir||T38347 aconitate hydratase
- fission yeast (Schizosaccharomyces pombe)
>gi|2330789|emb|CAB11263.1| (Z98601) aconitate hydratase
[Schizosaccharomyces pombe]
Length = 778
Score = 148 bits (371), Expect = 8e-35
Identities = 128/446 (28%), Positives = 207/446 (45%), Gaps = 40/446 (8%)
Query: 3 MTIAEKILADHSE---REEVKPG-EIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T +EKIL H + ++++ G + + D V D T +AI +F G+ V
Sbjct: 59 LTYSEKILYGHLDDPVNQDIERGVSYLKLRPDRVACQDATAQMAILQFMSAGMPEV--AV 116
Query: 59 RIAIVLDHFTPNK-----DIKSAEQC-KSSREFAKEMGIKW---FFEGGSVGVEHCLLPE 109
+ + DH D++ A K +F + K+ F+ GS G+ H ++ E
Sbjct: 117 PVTVHCDHLIEAYEGGPIDLERANVTNKEVYDFLQTACAKYNIGFWRPGS-GIIHQIVLE 175
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGEL 169
PG L+IG DSHT G LG A GVG D MA + P+ + G+L
Sbjct: 176 -NYAFPGGLLIGTDSHTPNAGGLGMVAIGVGGADAVDVMANLPWELKCPKVIGVKLTGQL 234
Query: 170 QPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIE 229
+ + + KD+IL G + V G ++E+ G +E LS T+ NM E GA T I
Sbjct: 235 KGWTSPKDVILKVAGILTVKGGTGAIVEYFGPGVESLSCTGMGTICNMGAEIGATTSIFP 294
Query: 230 PDKKTLDYVK--------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENT 281
+ + +Y++ + A+ + +DE+A Y ++IE D+ EP + P P+
Sbjct: 295 FNPRMSEYLRATNRSAIADYAEEFAPIIAADENAHYDQIIEIDLNTLEPHLNGPFTPDLA 354
Query: 282 VPISK---AAKMN---IKIDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIVI 331
PISK A K N ++ IGSCTN ED+ AA I + Q + K ++ +
Sbjct: 355 TPISKFKEAVKKNDWPQELKVGLIGSCTNSSYEDMSRAASICQ-QAIDKGIKTKSLFTIT 413
Query: 332 PCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGV-LASGER--AVSTTNRNFV 388
P S V ++G ++ +AG ++ CGPC+G + GE+ V++ NRNF
Sbjct: 414 PGSEQVRATLTRDGQLDTMRKAGGIVLANACGPCIGQWKRTDVKKGEKNSIVTSYNRNFT 473
Query: 389 GRM-GHPKSEVYLANPYVAAASAVLG 413
GR +P + ++ +P + A G
Sbjct: 474 GRNDANPATHAFVTSPDIVTAMVFSG 499
>gb|AAC61778.1| (AF093142) aconitase [Aspergillus terreus]
Length = 778
Score = 148 bits (370), Expect = 1e-34
Identities = 134/448 (29%), Positives = 204/448 (44%), Gaps = 40/448 (8%)
Query: 3 MTIAEKILADHSER---EEVKPG-EIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T AEK+L H + ++++ G + + D V D T +AI +F G+ V
Sbjct: 60 LTYAEKVLYSHLDDPHGQDIERGVSYLKLRPDRVACQDATAQMAILQFMSAGMPSV--AT 117
Query: 59 RIAIVLDHFTP-----NKDIKSAEQC-KSSREFAKEMGIKW---FFEGGSVGVEHCLLPE 109
+ DH KD+ A + K +F K+ F++ GS G+ H ++ E
Sbjct: 118 PTTVHCDHLIEAQVGGEKDLARANEINKEVYDFLATATAKYNIGFWKPGS-GIIHQIVLE 176
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G L A GVG D AV + G W + P+ + GE
Sbjct: 177 -NYAFPGGLMIGTDSHTPNAGGLAMAAIGVGGAD-AVDVMAGLPWELKAPKVIGVKLTGE 234
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
+ + T KD+IL G + V G ++E+ G + LS T+ NM E GA T +
Sbjct: 235 MSGWTTPKDIILKVAGLLTVKGGTGAIIEYHGPGVNSLSCTGMGTICNMGAEIGATTSMF 294
Query: 229 EPDKKTLDYVK--------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + DY+K E A+ K + DE A+Y ++IE +++ EP + P P+
Sbjct: 295 PFNDRMYDYLKATKRQHIGEFARSYAKELREDEGAEYDQLIEINLSELEPHINGPFTPDL 354
Query: 281 TVPISK---AAKMNIKIDQV---FIGSCTNGRIEDLRMAAEILE---GQKVAKWVRLIVI 331
PISK A N +++ IGSCTN ED+ AA I V V
Sbjct: 355 ATPISKFKEAVDANGWPEELKVGLIGSCTNSSYEDMSRAASIARDALNHGVKSKSLFTVT 414
Query: 332 PCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGE--RAVSTTNRNFV 388
P S + ++G ++ E G VI CGPC+G + GE +S+ NRNF
Sbjct: 415 PGSEQIRATIERDGQLQTLEEFGGVILANACGPCIGQWDRKDVKKGEPNSIISSYNRNFT 474
Query: 389 GRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P + ++A+P + A V G +
Sbjct: 475 GRNDANPATHAFVASPDLVVAMCVAGTL 502
>emb|CAB72317.1| (AJ245736) aconitase [Daphnia pulex]
Length = 788
Score = 147 bits (368), Expect = 2e-34
Identities = 130/450 (28%), Positives = 207/450 (45%), Gaps = 43/450 (9%)
Query: 3 MTIAEKILADH----SEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
MT++EKIL H S +E V+ + + D V D T +A+ +F G+ RV
Sbjct: 68 MTLSEKILYSHLDDPSNQEIVRGQSYLRLRPDRVAMQDATAQMAMLQFISSGLPRVAVPS 127
Query: 59 RIAIVLDHFTPNKDIKSAEQCKSSREFAKEM-------GIKW---FFEGGSVGVEHCLLP 108
I DH + A+ + + KE+ G K+ F++ GS G+ H ++
Sbjct: 128 TIHC--DHLI-EAQVGGAQDLARAVDINKEVYNFLSTSGAKYGVGFWKPGS-GIIHQIIL 183
Query: 109 ELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGE 168
E PG L+IG DSHT G LG GVG D MA + P+ + G+
Sbjct: 184 E-NYAFPGLLMIGTDSHTPNGGGLGGLCIGVGGADAVDVMANIPWELKCPKVIGVKLTGK 242
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
LQ + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 243 LQGWTSPKDVILKVAGILTVKGGTGAIVEYFGPGVDSISCTGMATICNMGAEIGATTSVF 302
Query: 229 EPDKKTLDYVKERAKRKF---------KVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPE 279
+ + DY+ ++ + ++DEDA Y +VIE +++ EP V P P+
Sbjct: 303 PYNHRMRDYLVATERKAIADEIDPYGNEFLRADEDAPYDQVIELNLSELEPHVNGPFTPD 362
Query: 280 NTVPISK---AAKMN---IKIDQVFIGSCTNGRIEDL----RMAAEILEGQKVAKWVRLI 329
PISK AK N + + IGSCTN ED+ +A E L+ AK
Sbjct: 363 LANPISKLGEEAKKNNWPLDVKVSLIGSCTNSSYEDMGRCASIAREALKHGLKAK-SAFN 421
Query: 330 VIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRN 386
+ P S + ++G+ E + G ++ CGPC+G + GE+ V++ NRN
Sbjct: 422 ITPGSEQIRATIERDGIAETLRQFGGIVLAQPCGPCIGQWDRKDVKKGEKNTIVTSYNRN 481
Query: 387 FVGRM-GHPKSEVYLANPYVAAASAVLGRI 415
F R +P + ++ +P + A A+ G +
Sbjct: 482 FTARNDANPATHAFVTSPELTTALALAGTL 511
>gi|6322261 Yjl200cp [Saccharomyces cerevisiae] >gi|728783|sp|P39533|ACOX_YEAST
PUTATIVE ACONITASE IN PRP21-UBP12 INTERGENIC REGION
>gi|630161|pir||S46631 aconitate hydratase homolog
YJL200c - yeast (Saccharomyces cerevisiae)
>gi|547592|emb|CAA54757.1| (X77688) hypothetical protein
J0327 [Saccharomyces cerevisiae]
>gi|1008419|emb|CAA89495.1| (Z49475) ORF YJL200c
[Saccharomyces cerevisiae]
Length = 789
Score = 147 bits (367), Expect = 2e-34
Identities = 129/458 (28%), Positives = 203/458 (44%), Gaps = 53/458 (11%)
Query: 3 MTIAEKILADH----------SEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVK 52
+T+AEKIL H S+ ++ + + D V D + +A+ +F G+
Sbjct: 54 LTLAEKILYSHLCDPEESITSSDLSTIRGNKYLKLNPDRVAMQDASAQMALLQFMTTGLN 113
Query: 53 RVFDRERIAIVLDHFTPNKDIKSAEQCKSS----------REFAKEMGIKWFFEGGSVGV 102
+ +I DH KD ++ + S AK GI+ F G G+
Sbjct: 114 QT--SVPASIHCDHLIVGKDGETKDLPSSIATNQEVFDFLESCAKRYGIQ--FWGPGSGI 169
Query: 103 EHCLLPELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETM 161
H ++ E PG +++G DSHT G LGA A GVG D AV TG W + P+ +
Sbjct: 170 IHQIVLE-NFSAPGLMMLGTDSHTPNAGGLGAIAIGVGGAD-AVDALTGTPWELKAPKIL 227
Query: 162 KFIYEGELQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEA 221
G+L + T KD+I G + V G ++E+ G + LS T+ NM E
Sbjct: 228 GVKLTGKLNGWSTPKDVITKLAGLLTVRGGTGYIVEYFGEGVSTLSCTGMATICNMGAEI 287
Query: 222 GAKTGIIEPDKKTLDYVK-----------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEP 270
GA T + Y++ + A KF ++D+DA+Y KVIE D++ EP
Sbjct: 288 GATTSTFPYQEAHKRYLQATNRAEVAEAADVALNKFNFLRADKDAQYDKVIEIDLSAIEP 347
Query: 271 VVAFPHLPENTVPISKAAKMNI------KIDQVFIGSCTNGRIEDLRMAAEILEGQKVA- 323
V P P+ + PIS+ A+ ++ K+ IGSCTN +D+ ++++ A
Sbjct: 348 HVNGPFTPDLSTPISQYAEKSLKENWPQKVSAGLIGSCTNSSYQDMSRVVDLVKQASKAG 407
Query: 324 --KWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGERAVS 381
+ V P S + ++G+I+IF E GA + CGPC+G S +
Sbjct: 408 LKPRIPFFVTPGSEQIRATLERDGIIDIFQENGAKVLANACGPCIGQWNREDVSKTSKET 467
Query: 382 TT-----NRNFVGRM-GHPKSEVYLANPYVAAASAVLG 413
T NRNF R G+ + +L +P + A + G
Sbjct: 468 NTIFTSFNRNFRARNDGNRNTMNFLTSPEIVTAMSYSG 505
>emb|CAB93521.1| (AJ252018) mitochondrial aconitase [Drosophila melanogaster]
>gi|10728908|gb|AAF53974.2| (AE003669) Acon gene product
[alt 2] [Drosophila melanogaster]
Length = 787
Score = 145 bits (362), Expect = 1e-33
Identities = 121/450 (26%), Positives = 207/450 (45%), Gaps = 43/450 (9%)
Query: 3 MTIAEKILADH----SEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EK+L H + ++ V+ + + D V D T +A+ +F G+K+V
Sbjct: 70 LTLSEKVLYSHLDDPANQDIVRGTSYLRLRPDRVAMQDATAQMALLQFISSGLKKVAVPS 129
Query: 59 RIAIVLDHFTPN-----KDIKSAEQC-KSSREFAKEMGIKW---FFEGGSVGVEHCLLPE 109
+ DH KD+ A+ K +F K+ F++ GS G+ H ++ E
Sbjct: 130 TVHC--DHLIEAQIGGPKDLARAKDLNKEVYDFLASTCAKYGLGFWKPGS-GIIHQIILE 186
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGEL 169
PG L+IG DSHT G LG GVG D MA + P+ + G++
Sbjct: 187 -NYAFPGLLMIGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVNLTGKI 245
Query: 170 QPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIE 229
+ + KD+IL + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 246 SGWTSPKDVILKVADILTVKGGTGAIIEYHGKGVDSISCTGMATICNMGAEIGATTSLFP 305
Query: 230 PDKKTLDYVK---------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+++ DY+K E K + K+ +D++ +Y ++IE ++ EP V P P+
Sbjct: 306 FNQRMADYLKSTGRAGIASEAQKYQAKILSADKNCEYDELIEINLDTLEPHVNGPFTPDL 365
Query: 281 TVPISKAAK--------MNIKIDQVFIGSCTNGRIEDLRMAAEILE---GQKVAKWVRLI 329
PISK + M+I++ IGSCTN ED+ A I + + +
Sbjct: 366 GHPISKLGENSKKNGYPMDIRVG--LIGSCTNSSYEDMGRCASIAKDAMSHGLKSKIPFN 423
Query: 330 VIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG--GHMGVLASGERAVSTT-NRN 386
V P S + ++G+ E+F + G + CGPC+G V + + T+ NRN
Sbjct: 424 VTPGSEQIRATIERDGISEVFDKFGGTVLANACGPCIGQWDRKDVKKGDKNTIVTSYNRN 483
Query: 387 FVGRM-GHPKSEVYLANPYVAAASAVLGRI 415
F GR +P + ++ +P + A ++ GR+
Sbjct: 484 FTGRNDANPATHCFVTSPELVTALSIAGRL 513
>emb|CAA88513.1| (Z48612) unknown [Saccharomyces cerevisiae]
Length = 324
Score = 140 bits (350), Expect = 2e-32
Identities = 91/311 (29%), Positives = 154/311 (49%), Gaps = 30/311 (9%)
Query: 2 GMTIAEKILADHS----EREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDR 57
G + EKI+ ++ E + V G+ V K +D + P+A+K F LG ++ +
Sbjct: 20 GQNLTEKIVQSYAVNLPEGKVVHSGDYVSIKPAHCMSHDNSWPVALK-FMGLGATKIKNP 78
Query: 58 ERIAIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGD 117
+I LDH NK K+ + K+ FAK+ I + G +G H ++ E G P +
Sbjct: 79 SQIVTTLDHDIQNKSEKNLTKYKNIENFAKKHHIDHYPAGRGIG--HQIMIEEGYAFPLN 136
Query: 118 LIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKD 177
+ + +DSH+ TYG LG+ T + TD A ATG+ W+++P + +G+L V+GKD
Sbjct: 137 MTVASDSHSNTYGGLGSLGTPIVRTDAAAIWATGQTWWQIPPVAQVELKGQLPQGVSGKD 196
Query: 178 LILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDY 237
+I+ G + L +EF+G + L ++ R+T++NM E GA +G+ DK +D+
Sbjct: 197 IIVALCGLFNNDQVLNHAIEFTGDSLNALPIDHRLTIANMTTEWGALSGLFPVDKTLIDW 256
Query: 238 VKERAKR-------------------KFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLP 278
K R ++ K K+ K+D+DA Y K + D+ V+ P
Sbjct: 257 YKNRLQKLGTNNHPRINPKTIRALEEKAKIPKADKDAHYAKKLIIDLATLTHYVSGP--- 313
Query: 279 ENTVPISKAAK 289
N+V +S +
Sbjct: 314 -NSVKVSNTVQ 323
>sp|P34455|ACON_CAEEL PROBABLE ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSOR (CITRATE
HYDRO-LYASE) (ACONITASE) >gi|630644|pir||S44831 F54H12.1
protein - Caenorhabditis elegans
>gi|409297|gb|AAA28050.1| (L25599) aconitate hydratase
[Caenorhabditis elegans]
Length = 788
Score = 138 bits (345), Expect = 9e-32
Identities = 124/449 (27%), Positives = 207/449 (45%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSEREEVKPGEIVMAKL----DFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKIL H ++ + + E ++ L D V D T +A+ +F G+ +
Sbjct: 71 LTLSEKILYGHLDQPKTQDIERGVSYLRLRPDRVAMQDATAQMAMLQFISSGLPKTAVPS 130
Query: 59 RIAIVLDHFTPNKDIKSAEQCKSSREFAKEM-------GIKW---FFEGGSVGVEHCLLP 108
I DH + A+ +++ KE+ G K+ F++ GS G+ H ++
Sbjct: 131 TIHC--DHLIEAQK-GGAQDLARAKDLNKEVFNFLATAGSKYGVGFWKPGS-GIIHQIIL 186
Query: 109 ELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGE 168
E PG L+IG DSHT G LG GVG D MA + P+ + G+
Sbjct: 187 E-NYAFPGLLLIGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGIKLTGK 245
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 246 LNGWTSAKDVILKVADILTVKGGTGAIVEYFGPGVDSISATGMGTICNMGAEIGATTSVF 305
Query: 229 EPDKKTLDY--------VKERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
++ Y + E A++ + +D+ A Y ++IE ++ P V P P+
Sbjct: 306 PYNESMYKYLEATGRKEIAEEARKYKDLLTADDGANYDQIIEINLDTLTPHVNGPFTPDL 365
Query: 281 TVPISKA---AKMN---IKIDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIV 330
I K AK N + + IGSCTN ED+ AA I + Q + K ++ +
Sbjct: 366 ASSIDKLGENAKKNGWPLDVKVSLIGSCTNSSYEDMTRAASIAK-QALDKGLKAKTIFTI 424
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S V ++GL +IF + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 425 TPGSEQVRATIERDGLSKIFADFGGMVLANACGPCIGQWDRQDVKKGEKNTIVTSYNRNF 484
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P + ++ +P + A A+ GR+
Sbjct: 485 TGRNDANPATHGFVTSPDITTAMAISGRL 513
>emb|CAA76360.1| (Y16747) aconitate hydratase [Piromyces sp. E2]
Length = 755
Score = 138 bits (345), Expect = 9e-32
Identities = 119/448 (26%), Positives = 209/448 (46%), Gaps = 40/448 (8%)
Query: 3 MTIAEKILADH---SEREEVKPGEIVM-AKLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T AEKI+ H + ++++ G+ + D V D T +A+ +F G+ V
Sbjct: 36 LTYAEKIVYGHLADPKNQDIERGKSYLNLHPDRVACQDATAQMALLQFMSAGLPTV--AV 93
Query: 59 RIAIVLDHFTP-----NKDIKSAEQC-KSSREFAKEMGIKW---FFEGGSVGVEHCLLPE 109
+ DH D+ A++ K +F K+ F++ GS G+ H ++ E
Sbjct: 94 PTTVHCDHLIQAQVGAESDLPRAKEINKEVYDFLSSCSAKYGIGFWKPGS-GIIHQIVLE 152
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG ++IG DSHT G LG A GVG D AV + G W + P+ + G+
Sbjct: 153 -NYAYPGGMMIGTDSHTPNAGGLGMIAVGVGGAD-AVDVMAGIPWELKCPKIIGVKLTGK 210
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
+ + D+IL G + V G ++E+ G +E +S T+ NM E GA T +
Sbjct: 211 RNGWTSPTDIILKVAGILTVKGGTGAIVEYFGEGVESISCTGMATICNMGAEIGATTSVF 270
Query: 229 EPDKKTLDYVK--------ERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+++ +Y+K E AK+ + K+DE Y ++IE +++ EP + P P+
Sbjct: 271 PFNERMEEYLKATGRSEIAELAKQYKDLLKADEGCHYDQIIEINLSELEPHINGPFTPDL 330
Query: 281 TVPISK---AAKMNIKIDQV---FIGSCTNGRIEDLRMAAEILE---GQKVAKWVRLIVI 331
P+S+ A + N +++ IGSCTN ED+ + +++ V + I+
Sbjct: 331 ATPLSEFKNAVEKNGWPEELKVGLIGSCTNSSYEDMCRVSSLIKQALDNGVPAKSKFIIT 390
Query: 332 PCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGE--RAVSTTNRNFV 388
P S + K+G +E+ + G V+ CGPC+G + G+ +++ NRNF
Sbjct: 391 PGSEQIRATIAKDGQMELMKKVGGVVLANACGPCIGQWDRKDIKKGDLNSIITSYNRNFT 450
Query: 389 GRM-GHPKSEVYLANPYVAAASAVLGRI 415
R +P++ ++A+P + A G +
Sbjct: 451 ARNDANPQTHAFVASPEIVTAMVFGGSL 478
>gb|AAF53973.1| (AE003669) Acon gene product [alt 1] [Drosophila melanogaster]
Length = 683
Score = 137 bits (342), Expect = 2e-31
Identities = 113/413 (27%), Positives = 189/413 (45%), Gaps = 39/413 (9%)
Query: 36 DVTMPLAIKKFRELGVKRVFDRERIAIVLDHFTPN-----KDIKSAEQC-KSSREFAKEM 89
D T +A+ +F G+K+V + DH KD+ A+ K +F
Sbjct: 3 DATAQMALLQFISSGLKKVAVPSTVHC--DHLIEAQIGGPKDLARAKDLNKEVYDFLAST 60
Query: 90 GIKW---FFEGGSVGVEHCLLPELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAV 146
K+ F++ GS G+ H ++ E PG L+IG DSHT G LG GVG D
Sbjct: 61 CAKYGLGFWKPGS-GIIHQIILE-NYAFPGLLMIGTDSHTPNGGGLGGLCIGVGGADAVD 118
Query: 147 AMATGEAWFRVPETMKFIYEGELQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEEL 206
MA + P+ + G++ + + KD+IL + V G ++E+ G ++ +
Sbjct: 119 VMADIPWELKCPKVIGVNLTGKISGWTSPKDVILKVADILTVKGGTGAIIEYHGKGVDSI 178
Query: 207 SVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVK---------ERAKRKFKVYKSDEDAKY 257
S T+ NM E GA T + +++ DY+K E K + K+ +D++ +Y
Sbjct: 179 SCTGMATICNMGAEIGATTSLFPFNQRMADYLKSTGRAGIASEAQKYQAKILSADKNCEY 238
Query: 258 YKVIEYDVTNWEPVVAFPHLPENTVPISKAAK--------MNIKIDQVFIGSCTNGRIED 309
++IE ++ EP V P P+ PISK + M+I++ IGSCTN ED
Sbjct: 239 DELIEINLDTLEPHVNGPFTPDLGHPISKLGENSKKNGYPMDIRVG--LIGSCTNSSYED 296
Query: 310 LRMAAEILE---GQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCL 366
+ A I + + + V P S + ++G+ E+F + G + CGPC+
Sbjct: 297 MGRCASIAKDAMSHGLKSKIPFNVTPGSEQIRATIERDGISEVFDKFGGTVLANACGPCI 356
Query: 367 G--GHMGVLASGERAVSTT-NRNFVGRM-GHPKSEVYLANPYVAAASAVLGRI 415
G V + + T+ NRNF GR +P + ++ +P + A ++ GR+
Sbjct: 357 GQWDRKDVKKGDKNTIVTSYNRNFTGRNDANPATHCFVTSPELVTALSIAGRL 409
>gi|4501867 aconitase 2; Aconitase, mitochondrial [Homo sapiens]
>gi|11418186|ref|XP_010018.1| aconitase 2 [Homo sapiens]
>gi|6686275|sp|Q99798|ACON_HUMAN ACONITATE HYDRATASE,
MITOCHONDRIAL PRECURSOR (CITRATE HYDRO-LYASE)
(ACONITASE) >gi|3366620|gb|AAC39921.1| (U87939)
aconitate hydratase [Homo sapiens]
Length = 780
Score = 135 bits (336), Expect = 1e-30
Identities = 119/449 (26%), Positives = 209/449 (46%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSE---REEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E++ G+ + + D V D T +A+ +F G+ +V
Sbjct: 63 LTLSEKIVYGHLDDPASQEIERGKSYLRLRPDRVAMQDATAQMAMLQFISSGLSKVAVPS 122
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD++ A+ F G K+ F++ GS G+ H ++ E
Sbjct: 123 TIHC--DHLIEAQVGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWKPGS-GIIHQIILE 179
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P+ + G
Sbjct: 180 -NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKCPKVIGVKLTGS 237
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 238 LSGWSSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVF 297
Query: 229 EPDKKTLDYVKERAKR-------KFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + Y+ + + +FK + D Y ++IE +++ +P + P P+
Sbjct: 298 PYNHRMKKYLSKTGREDIANLADEFKDHLVPDPGCHYDQLIEINLSELKPHINGPFTPDL 357
Query: 281 TVPISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIV 330
P+++ K+ K I IGSCTN ED+ +A + + Q +A ++ +
Sbjct: 358 AHPVAEVGKVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTI 416
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S + ++G +I + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 417 TPGSEQIRATIERDGYAQILRDLGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNF 476
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P++ ++ +P + A A+ G +
Sbjct: 477 TGRNDANPETHAFVTSPEIVTALAIAGTL 505
>emb|CAB46022.1| (AL023553) dJ347H13.1 (aconitase 2, mitochondrial (Aconitate
Hydratase, EC 4.2.1.3, Citrate Hydrolyase)) [Homo
sapiens]
Length = 768
Score = 135 bits (336), Expect = 1e-30
Identities = 119/449 (26%), Positives = 209/449 (46%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSE---REEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E++ G+ + + D V D T +A+ +F G+ +V
Sbjct: 51 LTLSEKIVYGHLDDPASQEIERGKSYLRLRPDRVAMQDATAQMAMLQFISSGLSKVAVPS 110
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD++ A+ F G K+ F++ GS G+ H ++ E
Sbjct: 111 TIHC--DHLIEAQVGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWKPGS-GIIHQIILE 167
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P+ + G
Sbjct: 168 -NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKCPKVIGVKLTGS 225
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 226 LSGWSSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVF 285
Query: 229 EPDKKTLDYVKERAKR-------KFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + Y+ + + +FK + D Y ++IE +++ +P + P P+
Sbjct: 286 PYNHRMKKYLSKTGREDIANLADEFKDHLVPDPGCHYDQLIEINLSELKPHINGPFTPDL 345
Query: 281 TVPISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIV 330
P+++ K+ K I IGSCTN ED+ +A + + Q +A ++ +
Sbjct: 346 AHPVAEVGKVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTI 404
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S + ++G +I + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 405 TPGSEQIRATIERDGYAQILRDLGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNF 464
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P++ ++ +P + A A+ G +
Sbjct: 465 TGRNDANPETHAFVTSPEIVTALAIAGTL 493
>sp|P16276|ACON_PIG ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSOR (CITRATE HYDRO-LYASE)
(ACONITASE) >gi|108227|pir||A35544 aconitate hydratase
(EC 4.2.1.3) precursor, mitochondrial [validated] - pig
>gi|164315|gb|AAA30987.1| (J05224) heart aconitase
precursor [Sus scrofa]
Length = 781
Score = 134 bits (335), Expect = 1e-30
Identities = 118/449 (26%), Positives = 207/449 (45%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSE---REEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E++ G+ + + D V D T +A+ +F G+ +V
Sbjct: 63 LTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPS 122
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD++ A+ F G K+ F+ GS G+ H ++ E
Sbjct: 123 TIHC--DHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGS-GIIHQIILE 179
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P+ + G
Sbjct: 180 -NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKCPKVIGVKLTGS 237
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 238 LSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVF 297
Query: 229 EPDKKTLDYVKERAKR-------KFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + Y+ + + +FK + D Y +VIE +++ +P + P P+
Sbjct: 298 PYNHRMKKYLSKTGRADIANLADEFKDHLVPDPGCHYDQVIEINLSELKPHINGPFTPDL 357
Query: 281 TVPISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIV 330
P+++ + K I IGSCTN ED+ +A + + Q +A ++ +
Sbjct: 358 AHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTI 416
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S + ++G ++ + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 417 TPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNF 476
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P++ ++ +P + A A+ G +
Sbjct: 477 TGRNDANPETHAFVTSPEIVTALAIAGTL 505
>pdb|5ACN| Aconitase (E.C.4.2.1.3) (Inactive (3fe-4s) Cluster Form)
>gi|231188|pdb|6ACN| Aconitase (E.C.4.2.1.3) (Activated
(4fe-4s) Cluster Form) >gi|443544|pdb|7ACN| Aconitase
(E.C.4.2.1.3) Complex With Isocitrate
Length = 754
Score = 134 bits (335), Expect = 1e-30
Identities = 118/449 (26%), Positives = 207/449 (45%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSE---REEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E++ G+ + + D V D T +A+ +F G+ +V
Sbjct: 36 LTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPS 95
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD++ A+ F G K+ F+ GS G+ H ++ E
Sbjct: 96 TIHC--DHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGS-GIIHQIILE 152
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P+ + G
Sbjct: 153 -NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKCPKVIGVKLTGS 210
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 211 LSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVF 270
Query: 229 EPDKKTLDYVKERAKR-------KFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + Y+ + + +FK + D Y +VIE +++ +P + P P+
Sbjct: 271 PYNHRMKKYLSKTGRADIANLADEFKDHLVPDPGCHYDQVIEINLSELKPHINGPFTPDL 330
Query: 281 TVPISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIV 330
P+++ + K I IGSCTN ED+ +A + + Q +A ++ +
Sbjct: 331 AHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTI 389
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S + ++G ++ + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 390 TPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNF 449
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P++ ++ +P + A A+ G +
Sbjct: 450 TGRNDANPETHAFVTSPEIVTALAIAGTL 478
>gb|AAB38416.1| (U80040) mitochondrial aconitase [Homo sapiens]
Length = 780
Score = 134 bits (335), Expect = 1e-30
Identities = 119/449 (26%), Positives = 208/449 (45%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSE---REEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E++ G+ + + D V D T +A+ +F G+ +V
Sbjct: 63 LTLSEKIVYGHLDDPASQEIERGKSYLRLRPDRVAMQDATAQMAMLQFISSGLSKVAVPS 122
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD++ A+ F G K+ F+ GS G+ H ++ E
Sbjct: 123 TIHC--DHLIEAQVGDEKDLRRAKDINQEVYNFLATAGDKYGVGFWSPGS-GIIHQIILE 179
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P+ + G
Sbjct: 180 -NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKCPKVIGVKLTGS 237
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 238 LSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSMSCTGMATICNMGAEIGATTSVF 297
Query: 229 EPDKKTLDYVKERAKR-------KFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + Y+ + + +FK + D Y ++IE +++ +P + P P+
Sbjct: 298 PYNHRMKKYLSKTGREDIANLADEFKDHLVPDPGCHYDQLIEINLSELKPHINGPFTPDL 357
Query: 281 TVPISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIV 330
P+++ K+ K I IGSCTN ED+ +A + + Q +A ++ +
Sbjct: 358 AHPVAEVGKVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTI 416
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S + ++G +I + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 417 TPGSEQIRATIERDGYAQILRDLGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNF 476
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P++ ++ +P + A A+ G +
Sbjct: 477 TGRNDANPETHAFVTSPEIVTALAIAGTL 505
>pdb|1C96|A Chain A, S642a:citrate Complex Of Aconitase
Length = 753
Score = 134 bits (335), Expect = 1e-30
Identities = 118/449 (26%), Positives = 207/449 (45%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSE---REEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E++ G+ + + D V D T +A+ +F G+ +V
Sbjct: 35 LTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPS 94
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD++ A+ F G K+ F+ GS G+ H ++ E
Sbjct: 95 TIHC--DHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGS-GIIHQIILE 151
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P+ + G
Sbjct: 152 -NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKCPKVIGVKLTGS 209
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 210 LSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVF 269
Query: 229 EPDKKTLDYVKERAKR-------KFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + Y+ + + +FK + D Y +VIE +++ +P + P P+
Sbjct: 270 PYNHRMKKYLSKTGRADIANLADEFKDHLVPDPGCHYDQVIEINLSELKPHINGPFTPDL 329
Query: 281 TVPISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIV 330
P+++ + K I IGSCTN ED+ +A + + Q +A ++ +
Sbjct: 330 AHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTI 388
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S + ++G ++ + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 389 TPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNF 448
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P++ ++ +P + A A+ G +
Sbjct: 449 TGRNDANPETHAFVTSPEIVTALAIAGTL 477
>pdb|1C97|A Chain A, S642a:isocitrate Complex Of Aconitase
>gi|6729979|pdb|1B0K|A Chain A, S642a:fluorocitrate
Complex Of Aconitase
Length = 753
Score = 134 bits (335), Expect = 1e-30
Identities = 118/449 (26%), Positives = 207/449 (45%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSE---REEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E++ G+ + + D V D T +A+ +F G+ +V
Sbjct: 35 LTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPS 94
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD++ A+ F G K+ F+ GS G+ H ++ E
Sbjct: 95 TIHC--DHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGS-GIIHQIILE 151
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P+ + G
Sbjct: 152 -NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKCPKVIGVKLTGS 209
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 210 LSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVF 269
Query: 229 EPDKKTLDYVKERAKR-------KFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + Y+ + + +FK + D Y +VIE +++ +P + P P+
Sbjct: 270 PYNHRMKKYLSKTGRADIANLADEFKDHLVPDPGCHYDQVIEINLSELKPHINGPFTPDL 329
Query: 281 TVPISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIV 330
P+++ + K I IGSCTN ED+ +A + + Q +A ++ +
Sbjct: 330 AHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTI 388
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S + ++G ++ + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 389 TPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNF 448
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P++ ++ +P + A A+ G +
Sbjct: 449 TGRNDANPETHAFVTSPEIVTALAIAGTL 477
>pdb|1B0J|A Chain A, Crystal Structure Of Aconitase With Isocitrate
Length = 754
Score = 134 bits (335), Expect = 1e-30
Identities = 118/449 (26%), Positives = 207/449 (45%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSE---REEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E++ G+ + + D V D T +A+ +F G+ +V
Sbjct: 36 LTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPS 95
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD++ A+ F G K+ F+ GS G+ H ++ E
Sbjct: 96 TIHC--DHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGS-GIIHQIILE 152
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P+ + G
Sbjct: 153 -NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKCPKVIGVKLTGS 210
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 211 LSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVF 270
Query: 229 EPDKKTLDYVKERAKR-------KFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + Y+ + + +FK + D Y +VIE +++ +P + P P+
Sbjct: 271 PYNHRMKKYLSKTGRADIANLADEFKDHLVPDPGCHYDQVIEINLSELKPHINGPFTPDL 330
Query: 281 TVPISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIV 330
P+++ + K I IGSCTN ED+ +A + + Q +A ++ +
Sbjct: 331 AHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTI 389
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S + ++G ++ + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 390 TPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNF 449
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P++ ++ +P + A A+ G +
Sbjct: 450 TGRNDANPETHAFVTSPEIVTALAIAGTL 478
>pdb|1B0M|A Chain A, Aconitase R644q:fluorocitrate Complex
Length = 753
Score = 134 bits (335), Expect = 1e-30
Identities = 118/449 (26%), Positives = 207/449 (45%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSE---REEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E++ G+ + + D V D T +A+ +F G+ +V
Sbjct: 35 LTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPS 94
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD++ A+ F G K+ F+ GS G+ H ++ E
Sbjct: 95 TIHC--DHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGS-GIIHQIILE 151
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P+ + G
Sbjct: 152 -NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKCPKVIGVKLTGS 209
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 210 LSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVF 269
Query: 229 EPDKKTLDYVKERAKR-------KFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + Y+ + + +FK + D Y +VIE +++ +P + P P+
Sbjct: 270 PYNHRMKKYLSKTGRADIANLADEFKDHLVPDPGCHYDQVIEINLSELKPHINGPFTPDL 329
Query: 281 TVPISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIV 330
P+++ + K I IGSCTN ED+ +A + + Q +A ++ +
Sbjct: 330 AHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTI 388
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S + ++G ++ + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 389 TPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNF 448
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P++ ++ +P + A A+ G +
Sbjct: 449 TGRNDANPETHAFVTSPEIVTALAIAGTL 477
>sp|P20004|ACON_BOVIN ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSOR (CITRATE HYDRO-LYASE)
(ACONITASE) >gi|1364216|emb|CAA90177.1| (Z49931)
aconitase [Bos taurus]
Length = 780
Score = 134 bits (334), Expect = 2e-30
Identities = 118/449 (26%), Positives = 207/449 (45%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSE---REEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E++ G+ + + D V D T +A+ +F G+ +V
Sbjct: 63 LTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMRDATAQMAMLQFISSGLPKVAVPS 122
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD++ A+ F G K+ F+ GS G+ H ++ E
Sbjct: 123 TIHC--DHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGS-GIIHQIILE 179
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P+ + G
Sbjct: 180 -NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVTAGIPWELKCPKVIGVKLTGS 237
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 238 LSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVF 297
Query: 229 EPDKKTLDYVKERAKR-------KFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + Y+ + + +FK + D Y ++IE +++ +P + P P+
Sbjct: 298 PYNHRMKKYLSKTGRADIANLADEFKDHLVPDSGCHYDQLIEINLSELKPHINGPFTPDL 357
Query: 281 TVPISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWV----RLIV 330
P+++ + K I IGSCTN ED+ +A + + Q +A + + +
Sbjct: 358 AHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLQCKSQFTI 416
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S + ++G +I + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 417 TPGSEQIRATIERDGYAQILRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNF 476
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P++ ++ +P + A A+ G +
Sbjct: 477 TGRNDANPETHAFVTSPEIVTALAIAGTL 505
>emb|CAC18623.1| (AL451109) related to aconitate hydratase precursor [Neurospora
crassa]
Length = 809
Score = 134 bits (334), Expect = 2e-30
Identities = 122/464 (26%), Positives = 207/464 (44%), Gaps = 56/464 (12%)
Query: 3 MTIAEKILADHSEREE------------VKPGEIVMAKLDFVFGNDVTMPLAIKKFRELG 50
+T+AEKIL H + E V+ + K D V D + +A+ +F
Sbjct: 69 LTLAEKILYSHLDNVEESLLSNTNGGRDVRGKANLKLKPDRVNMQDASAQMALLQFMTCN 128
Query: 51 VKRVFDRERIA---IVLDHFTPNKDIKSAEQCKSSR-EFAKEMGIKW---FFEGGSVGVE 103
+ + I +++ ++D+K+ + +F + G K+ F+ G+ G+
Sbjct: 129 LPQPVIPASIHCDHLIVGSKGADEDLKAGIATNNEVFDFLESAGKKYGIDFWPPGA-GII 187
Query: 104 HCLLPELGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMK 162
H + E +PG +++G DSH+ G L GVG D AV G W + P+ +
Sbjct: 188 HQTVLE-NYAVPGLMMLGTDSHSPNAGGLTTITIGVGGAD-AVEALVGAPWELKAPKILG 245
Query: 163 FIYEGELQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAG 222
G+L +V+ KD+ILH G + V G ++E+ G IE LS T+ NM E G
Sbjct: 246 VRLTGKLNDWVSAKDIILHLTGRLTVRGGTGYIVEYFGPGIESLSCSGSSTLCNMGAEIG 305
Query: 223 AKTGII------------------EPDKKTLD-YVKERAKRKFKVYKSDEDAKYYKVIEY 263
A T I + D K + + K + F +K+DE A+Y ++IE
Sbjct: 306 ATTSIFPFTEASSRYLKATNREQADKDAKNFENFTKGTGQDSFFKFKADEGAQYDELIEI 365
Query: 264 DVTNWEPVVAFPHLPENTVPIS------KAAKMNIKIDQVFIGSCTNGRIEDLRMAAEIL 317
D++ EP + P P+ ++P+S + K K+ IGSCTN ED+ I+
Sbjct: 366 DLSKLEPHINGPFTPDLSIPLSEFKKTVQEQKWPEKLSAGLIGSCTNSSYEDMTRCESIV 425
Query: 318 EGQK---VAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG---GHMG 371
+ + + + P S + ++ ++ F +AG ++ CGPC+G
Sbjct: 426 KAAEQAGIKPASDFYITPGSEGIRATLERDNTLQTFEKAGGIVLSNACGPCIGQWKRQDD 485
Query: 372 VLASGERAVSTT-NRNFVGRM-GHPKSEVYLANPYVAAASAVLG 413
+ A+ T+ NRNF GR G+ ++ +LA+P + A A G
Sbjct: 486 IPKGTPNAILTSYNRNFRGRNDGNLETMNFLASPEIVTAMAYAG 529
>pdb|8ACN| Aconitase (E.C.4.2.1.3) Complex With Nitroisocitrate
>gi|442617|pdb|1ACO| Aconitase (Mitochondrial)
(E.C.4.2.1.3) Complex With Transaconitate
>gi|1942843|pdb|1FGH| Complex With
4-Hydroxy-Trans-Aconitate
Length = 754
Score = 134 bits (333), Expect = 2e-30
Identities = 117/449 (26%), Positives = 207/449 (46%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSE---REEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E++ G+ + + D V D T +A+ +F G+ +V
Sbjct: 36 LTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPS 95
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD++ A+ F G K+ F+ GS G+ H ++ E
Sbjct: 96 TIHC--DHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGS-GIIHQIILE 152
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P+ + G
Sbjct: 153 -NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKCPKVIGVKLTGS 210
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 211 LSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVF 270
Query: 229 EPDKKTLDYVKERAKR-------KFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + Y+ + + +FK + D Y ++IE +++ +P + P P+
Sbjct: 271 PYNHRMKKYLSKTGRADIANLADEFKDHLVPDSGCHYDQLIEINLSELKPHINGPFTPDL 330
Query: 281 TVPISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIV 330
P+++ + K I IGSCTN ED+ +A + + Q +A ++ +
Sbjct: 331 AHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTI 389
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S + ++G ++ + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 390 TPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNF 449
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P++ ++ +P + A A+ G +
Sbjct: 450 TGRNDANPETHAFVTSPEIVTALAIAGTL 478
>pdb|1NIS| Aconitase (E.C.4.2.1.3) Complex With Nitrocitrate (Major Occupancy
Form) >gi|443149|pdb|1NIT| Aconitase (E.C.4.2.1.3)
Complex With Sulfate (Minor Occupancy Form)
Length = 754
Score = 134 bits (333), Expect = 2e-30
Identities = 117/449 (26%), Positives = 207/449 (46%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSE---REEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E++ G+ + + D V D T +A+ +F G+ +V
Sbjct: 36 LTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPS 95
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD++ A+ F G K+ F+ GS G+ H ++ E
Sbjct: 96 TIHC--DHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGS-GIIHQIILE 152
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P+ + G
Sbjct: 153 -NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKCPKVIGVKLTGS 210
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 211 LSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVF 270
Query: 229 EPDKKTLDYVKERAKR-------KFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + Y+ + + +FK + D Y ++IE +++ +P + P P+
Sbjct: 271 PYNHRMKKYLSKTGRADIANLADEFKDHLVPDSGCHYDQLIEINLSELKPHINGPFTPDL 330
Query: 281 TVPISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIV 330
P+++ + K I IGSCTN ED+ +A + + Q +A ++ +
Sbjct: 331 AHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTI 389
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S + ++G ++ + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 390 TPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNF 449
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P++ ++ +P + A A+ G +
Sbjct: 450 TGRNDANPETHAFVTSPEIVTALAIAGTL 478
>pdb|1AMJ| Aconitase (E.C.4.2.1.3) Complexed With Sulfate And Hydroxide
>gi|999984|pdb|1AMI| Aconitase (E.C.4.2.1.3) Complexed
With Alpha-Methyl-Isocitrate
Length = 754
Score = 134 bits (333), Expect = 2e-30
Identities = 117/449 (26%), Positives = 207/449 (46%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSE---REEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E++ G+ + + D V D T +A+ +F G+ +V
Sbjct: 36 LTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPS 95
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD++ A+ F G K+ F+ GS G+ H ++ E
Sbjct: 96 TIHC--DHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGS-GIIHQIILE 152
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P+ + G
Sbjct: 153 -NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKCPKVIGVKLTGS 210
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 211 LSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVF 270
Query: 229 EPDKKTLDYVKERAKR-------KFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + Y+ + + +FK + D Y ++IE +++ +P + P P+
Sbjct: 271 PYNHRMKKYLSKTGRADIANLADEFKDHLVPDSGCHYDQLIEINLSELKPHINGPFTPDL 330
Query: 281 TVPISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIV 330
P+++ + K I IGSCTN ED+ +A + + Q +A ++ +
Sbjct: 331 AHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTI 389
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S + ++G ++ + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 390 TPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNF 449
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P++ ++ +P + A A+ G +
Sbjct: 450 TGRNDANPETHAFVTSPEIVTALAIAGTL 478
>pir||S57528 aconitate hydratase (EC 4.2.1.3) precursor, mitochondrial
[validated] - bovine
Length = 781
Score = 134 bits (333), Expect = 2e-30
Identities = 117/449 (26%), Positives = 207/449 (46%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSE---REEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E++ G+ + + D V D T +A+ +F G+ +V
Sbjct: 63 LTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPS 122
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD++ A+ F G K+ F+ GS G+ H ++ E
Sbjct: 123 TIHC--DHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGS-GIIHQIILE 179
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P+ + G
Sbjct: 180 -NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKCPKVIGVKLTGS 237
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 238 LSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVF 297
Query: 229 EPDKKTLDYVKERAKR-------KFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + Y+ + + +FK + D Y ++IE +++ +P + P P+
Sbjct: 298 PYNHRMKKYLSKTGRADIANLADEFKDHLVPDSGCHYDQLIEINLSELKPHINGPFTPDL 357
Query: 281 TVPISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIV 330
P+++ + K I IGSCTN ED+ +A + + Q +A ++ +
Sbjct: 358 AHPVAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTI 416
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S + ++G ++ + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 417 TPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNF 476
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P++ ++ +P + A A+ G +
Sbjct: 477 TGRNDANPETHAFVTSPEIVTALAIAGTL 505
>gb|AAD19351.2| (AF093096) aconitase [Homo sapiens]
Length = 780
Score = 134 bits (333), Expect = 2e-30
Identities = 119/449 (26%), Positives = 208/449 (45%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSE---REEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E++ G+ + + D V D T +A+ +F G+ +V
Sbjct: 63 LTLSEKIVYGHLDDPASQEIERGKSYLRLRPDRVAMQDATAQMAMLQFISSGLSKVAVPS 122
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD++ A+ F G K+ F+ GS G+ H ++ E
Sbjct: 123 TIHC--DHLIEAQVGDEKDLRRAKDINQEVYNFLATAGDKYGVGFWSPGS-GIIHQIILE 179
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P+ + G
Sbjct: 180 -NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKCPKVIGVKLTGS 237
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 238 LSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSMSCTGMATICNMGAEIGATTSVF 297
Query: 229 EPDKKTLDYVKERAKR-------KFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + Y+ + + +FK + D Y ++IE +++ +P + P P+
Sbjct: 298 PYNHRMKKYLSKTGREDIANLADEFKDHLVPDPGCHYDQLIEINLSELKPHINGPFTPDL 357
Query: 281 TVPISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIV 330
P+++ K+ K I IGSCTN ED+ +A + + Q +A ++ +
Sbjct: 358 AHPVAEVGKVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTI 416
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S + ++G +I + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 417 TPGSEQIRATIERDGYRQILRDLGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNF 476
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P++ ++ +P + A A+ G +
Sbjct: 477 TGRNDANPETHAFVTSPEIVTALAIAGTL 505
>sp|Q44023|LEU2_ALCEU 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (ISOPROPYLMALATE
ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI)
>gi|2120952|pir||I39573 probable 3-isopropylmalate
dehydratase (EC 4.2.1.33) large chain [similarity] -
Alcaligenes eutrophus (fragment)
>gi|695283|gb|AAC41429.1| (L36817) ORF11 [Ralstonia
eutropha] >gi|1093470|prf||2104199L ORF 11 [Ralstonia
eutropha]
Length = 267
Score = 133 bits (331), Expect = 4e-30
Identities = 90/262 (34%), Positives = 121/262 (45%), Gaps = 41/262 (15%)
Query: 197 EFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYVKER-----------AKRK 245
EF GS + L VE R+T+ N+A+E A +GI+ PD +Y+ R A
Sbjct: 1 EFGGSAVRSLPVEARLTLCNLAVEFSAFSGIVAPDDTVFEYLAGRPYAPAGAQWEPALWH 60
Query: 246 FKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISKAAK---------------- 289
++ SD DA + + + D P+V + P++ V + A
Sbjct: 61 WRSLYSDADAVFDRELSVDCRQLAPMVTWGTSPQHGVAVDGAVPNPAMAIDADTRQAMER 120
Query: 290 -------------MNIKIDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVRLIVIPCSPT 336
+I ID FIGSCTN R+ DLR AA +L G+KVA V I +P S
Sbjct: 121 ALSYMDLRPGQRMADIAIDAAFIGSCTNSRLSDLRSAAGVLAGRKVAPGVTAICVPVSSA 180
Query: 337 VYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKS 396
V A EGL +F EAG C C +R +S+TNRNF R G P++
Sbjct: 181 VKRAAEAEGLDRVFREAGFEWRESGCSMCFYAGGESFGHRQRVISSTNRNFESRQG-PQT 239
Query: 397 EVYLANPYVAAASAVLGRIASP 418
+LA P AASAVLGRIA P
Sbjct: 240 RTHLAGPATVAASAVLGRIADP 261
>emb|CAC11018.1| (AJ243266) mitochondrial aconitase [Rattus norvegicus]
Length = 780
Score = 132 bits (329), Expect = 7e-30
Identities = 119/449 (26%), Positives = 206/449 (45%), Gaps = 42/449 (9%)
Query: 3 MTIAEKILADHSE---REEVKPGEIVMA-KLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E++ G+ + + D V D T +A+ +F G+ +V
Sbjct: 63 LTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPS 122
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD++ A+ F G K+ F+ GS G+ H ++ E
Sbjct: 123 TIHC--DHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGS-GIIHQIILE 179
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWF-RVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P+ + G
Sbjct: 180 -NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWEPKCPKVIGVKLTGT 237
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 238 LSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTPVF 297
Query: 229 EPDKKTLDYVKERAK-------RKFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPEN 280
+ + Y+ + + +FK + D +Y +VIE ++ +P + P P+
Sbjct: 298 PYNHRMKKYLSKTGRADIANLAEEFKDHLVPDPGCQYDQVIEINLNELKPHINGPFTPDL 357
Query: 281 TVPISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIV 330
P++ + K I IGSCTN ED+ +A + + Q +A ++ +
Sbjct: 358 AHPVADVGTVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTI 416
Query: 331 IPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTTNRNF 387
P S + ++G +I + G ++ CGPC+G + GE+ V++ NRNF
Sbjct: 417 TPGSEQIRATIERDGYAQILRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNF 476
Query: 388 VGRM-GHPKSEVYLANPYVAAASAVLGRI 415
GR +P++ ++ +P + A A+ G +
Sbjct: 477 TGRNDANPETHAFVTSPEIVTALAIAGTL 505
>gb|AAF01428.1|AF186471_1 (AF186471) aconitase [Xenopus laevis]
Length = 856
Score = 129 bits (322), Expect = 5e-29
Identities = 118/453 (26%), Positives = 200/453 (44%), Gaps = 49/453 (10%)
Query: 3 MTIAEKILADHSE----REEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
+T++EKI+ H + +E V+ + + D V D T +A+ +F G+ RV
Sbjct: 65 LTLSEKIVYGHIDDPVSQEIVRGKTYLRLRPDRVAMQDATAQMAMLQFISSGLARVAVPS 124
Query: 59 RIAIVLDHFTP-----NKDIKSAEQCKSS-REFAKEMGIKW---FFEGGSVGVEHCLLPE 109
I DH KD+K A+ F K+ F++ GS G+ H ++ E
Sbjct: 125 TIHC--DHLIEAQLGGEKDLKRAKDINQEVYNFLATAAAKYGVGFWKPGS-GIIHQIILE 181
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAW-FRVPETMKFIYEGE 168
PG L+IG DSHT G LG GVG D AV + G W + P + G+
Sbjct: 182 -NYAFPGVLLIGTDSHTPNGGGLGGICIGVGGAD-AVDVMAGIPWELKCPNVIGVKLTGQ 239
Query: 169 LQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGII 228
L + + KD+IL G + V G ++E+ G ++ +S T+ NM E GA T +
Sbjct: 240 LSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVF 299
Query: 229 EPDKKTLDYVKERAKR---------KFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPE 279
+ + Y+ + + K + + KY ++IE ++ +P + P P+
Sbjct: 300 PYNHRMKKYLDKTGRSEIASLTDEFKSNLVPDEGLLKYDQLIEINLDELKPHINGPFTPD 359
Query: 280 NTVPI--------SKAAKMNIKIDQVFIGSCTNGRIEDLRMAAEIL-----EGQKVAKWV 326
P+ K ++I++ IGSCTN ED+ AA + G K
Sbjct: 360 LANPVWEVGAVAEQKGWPLDIRVG--LIGSCTNSSYEDMGRAAAVARQALDHGLKCKS-- 415
Query: 327 RLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG-GHMGVLASGER--AVSTT 383
+ + P S + ++G + + G V+ CGPC+G + GE+ V++
Sbjct: 416 QFTITPGSEQIRATIERDGYAAVLRDVGGVVLANACGPCIGQWDRKDIKKGEKNTIVTSY 475
Query: 384 NRNFVGRM-GHPKSEVYLANPYVAAASAVLGRI 415
NRNF GR +P++ ++ +P + A ++ G +
Sbjct: 476 NRNFTGRNDANPETHAFVTSPEIVTALSIAGTL 508
>pir||S45196 3-isopropylmalate dehydrogenase homolog - Escherichia coli
>gi|473786|dbj|BAA05575.1| (D26562) 'ORF' [Escherichia
coli]
Length = 773
Score = 126 bits (313), Expect = 5e-28
Identities = 129/463 (27%), Positives = 211/463 (44%), Gaps = 62/463 (13%)
Query: 2 GMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIA 61
G ++A+K++ + ++PG K+ V D T P+ + ++L F + +
Sbjct: 289 GFSLAQKMVGRACGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLG-FSADLVM 347
Query: 62 IVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLIIG 121
H + +F G G GV H L + +LP + G
Sbjct: 348 QSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGD--GVIHSWLNRM--LLPDTVGTG 403
Query: 122 ADSHTCTYGALGAFATGV----GSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKD 177
DSHT F G+ GS +A A ATG +PE++ ++G++QP +T +D
Sbjct: 404 GDSHT-------RFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRD 456
Query: 178 LILHTIGDIGVNGALYKVME------FSGSVIE-----ELSVEQRMTMSNMAIEAGA--- 223
L+ H I + L V + FSG ++E +L VEQ +++ + E A
Sbjct: 457 LV-HAIPLYAIKQGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGC 515
Query: 224 -----KTGIIE-----------------PDKKTLD---YVKERAKRKFKVYKSDEDAKYY 258
K IIE D++TL+ E+ ++ ++D DA+Y
Sbjct: 516 TIKLNKEPIIEYLNSNIVLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYA 575
Query: 259 KVIEYDVTNW-EPVVAFPHLPENTVPISKAAKMNIKIDQVFIGSCTNGRIEDLRMAAEIL 317
VI+ D+ + EP++ P+ P++ P+S A KID+VFIGSC I R A ++L
Sbjct: 576 AVIDIDLADIKEPILCAPNDPDDARPLS--AVQGEKIDEVFIGSCMTN-IGHFRAAGKLL 632
Query: 318 EGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGE 377
+ K RL V P + + +EG +F ++GA I P C C+G V A G
Sbjct: 633 DAHKGQLPTRLWVAPPTRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARV-ADGA 691
Query: 378 RAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEE 420
VST+ RNF R+G + V+LA+ +AA +A++G++ +PEE
Sbjct: 692 TVVSTSTRNFPNRLG-TGANVFLASAELAAVAALIGKLPTPEE 733
>sp|P36683|ACO2_ECOLI ACONITATE HYDRATASE 2 (CITRATE HYDRO-LYASE 2) (ACONITASE 2)
>gi|7465770|pir||F64734 aconitate hydratase (EC 4.2.1.3)
2 - Escherichia coli >gi|2367097|gb|AAC73229.1|
(AE000121) aconitate hydrase B [Escherichia coli K12]
Length = 865
Score = 126 bits (313), Expect = 5e-28
Identities = 129/463 (27%), Positives = 211/463 (44%), Gaps = 62/463 (13%)
Query: 2 GMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIA 61
G ++A+K++ + ++PG K+ V D T P+ + ++L F + +
Sbjct: 381 GFSLAQKMVGRACGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLG-FSADLVM 439
Query: 62 IVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLIIG 121
H + +F G G GV H L + +LP + G
Sbjct: 440 QSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGD--GVIHSWLNRM--LLPDTVGTG 495
Query: 122 ADSHTCTYGALGAFATGV----GSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKD 177
DSHT F G+ GS +A A ATG +PE++ ++G++QP +T +D
Sbjct: 496 GDSHT-------RFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRD 548
Query: 178 LILHTIGDIGVNGALYKVME------FSGSVIE-----ELSVEQRMTMSNMAIEAGA--- 223
L+ H I + L V + FSG ++E +L VEQ +++ + E A
Sbjct: 549 LV-HAIPLYAIKQGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGC 607
Query: 224 -----KTGIIE-----------------PDKKTLD---YVKERAKRKFKVYKSDEDAKYY 258
K IIE D++TL+ E+ ++ ++D DA+Y
Sbjct: 608 TIKLNKEPIIEYLNSNIVLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYA 667
Query: 259 KVIEYDVTNW-EPVVAFPHLPENTVPISKAAKMNIKIDQVFIGSCTNGRIEDLRMAAEIL 317
VI+ D+ + EP++ P+ P++ P+S A KID+VFIGSC I R A ++L
Sbjct: 668 AVIDIDLADIKEPILCAPNDPDDARPLS--AVQGEKIDEVFIGSCMTN-IGHFRAAGKLL 724
Query: 318 EGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGE 377
+ K RL V P + + +EG +F ++GA I P C C+G V A G
Sbjct: 725 DAHKGQLPTRLWVAPPTRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARV-ADGA 783
Query: 378 RAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEE 420
VST+ RNF R+G + V+LA+ +AA +A++G++ +PEE
Sbjct: 784 TVVSTSTRNFPNRLG-TGANVFLASAELAAVAALIGKLPTPEE 825
>pir||C81356 aconitate hydratase (EC 4.2.1.3) Cj0835c [imported] - Campylobacter
jejuni (strain NCTC 11168) >gi|6968281|emb|CAB73100.1|
(AL139076) aconitate hydratase [Campylobacter jejuni]
Length = 848
Score = 123 bits (307), Expect = 3e-27
Identities = 136/469 (28%), Positives = 209/469 (43%), Gaps = 64/469 (13%)
Query: 2 GMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIA 61
G T+A+K+L E V+PG + V D T P+ + +EL F+ + +
Sbjct: 367 GYTLAQKMLGRACGVEGVRPGMYIEPITLTVGSQDTTGPMTRDEIKELA-SLGFNADFVM 425
Query: 62 IVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLIIG 121
H + + K+ F G G GV H L VLP + G
Sbjct: 426 QSFCHTAAYPKVSDSNLHKTLPNFMTSRGGVSLKPGD--GVIHSWLNRF--VLPDTVGTG 481
Query: 122 ADSHTCTYGALGAFATGV----GSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKD 177
DSHT F G+ GS +A A TG VPE++ + GELQ VT +D
Sbjct: 482 GDSHT-------RFPIGISFPAGSGLVAFAAVTGSMPLNVPESVLVRFSGELQAGVTLRD 534
Query: 178 LI----LHTI--GDIGVNGALYKVMEFSGSVIE-----ELSVEQRMTMSNMAIEAGAKTG 226
L+ + I G + V K + F+G ++E L VEQ +S+ + E A
Sbjct: 535 LVNAIPYYAIKQGQLTVEKKNKKNI-FAGKILEIEGLPNLKVEQAFELSDASAERSAAAC 593
Query: 227 IIEPDKKTL-DYVKER------------------AKRKFK---------VYKSDEDAKYY 258
++ +++ +Y+K A+R K + ++D+DAKY
Sbjct: 594 SVDLSIESVSEYIKSNISLIEAMIEVGYENKATLARRAEKMREWLKNPTLLRADKDAKYA 653
Query: 259 KVIEYDVTN-WEPVVAFPHLPENTVPISKAA---KMNIKIDQVFIGSCTNGRIEDLRMAA 314
+I+ ++ N EP++A P+ P++ +S+ K ID+VF+GSC I R
Sbjct: 654 YIIDINLNNIKEPILACPNDPDDVATLSEILADDKRPKNIDEVFVGSCMT-NIGHYRALG 712
Query: 315 EILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLA 374
EIL+ + + K RL V+P + + + EG IF AGA I P C C+G V
Sbjct: 713 EILKDKGILK-TRLWVVPPTKMDKAQLINEGYYSIFGAAGARIEVPGCSLCMGNQARV-N 770
Query: 375 SGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEEVVK 423
G ST+ RNF RMG ++VYL + +AA A+LG+I S EE ++
Sbjct: 771 DGAVVFSTSTRNFDNRMG-MGAKVYLGSAELAAVCAILGKIPSKEEYLQ 818
>pir||G83422 aconitate hydratase 2 PA1787 [imported] - Pseudomonas aeruginosa
(strain PAO1) >gi|9947768|gb|AAG05176.1|AE004604_6
(AE004604) aconitate hydratase 2 [Pseudomonas
aeruginosa]
Length = 869
Score = 122 bits (303), Expect = 8e-27
Identities = 129/466 (27%), Positives = 212/466 (44%), Gaps = 65/466 (13%)
Query: 2 GMTIAEKILADHS---EREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
G T+A+K++ E + V+PG K+ V D T P+ + ++L F +
Sbjct: 381 GFTLAQKMVGRACGLPEGQGVRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLG-FSAD 439
Query: 59 RIAIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDL 118
+ H + + +F G G G+ H L + +LP +
Sbjct: 440 LVMQSFCHTAAYPKPIDVKTHHTLPDFIMTRGGVSLRPGD--GIIHSWLNRM--LLPDTV 495
Query: 119 IIGADSHTCTYGALGAFATGV----GSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVT 174
G DSHT F G+ GS +A A ATG +PE++ ++G+LQP +T
Sbjct: 496 GTGGDSHT-------RFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKLQPGIT 548
Query: 175 GKDLILHTIGDIGVNGALYKVME------FSGSVIE-----ELSVEQRMTMSNMAIEAGA 223
+DL+ H I + L V + FSG ++E +L+VEQ +S+ + E A
Sbjct: 549 LRDLV-HAIPYYAIQQGLLTVEKKGKKNIFSGRILEIEGLNDLTVEQAFELSDASAERSA 607
Query: 224 KTGIIE-PDKKTLDYVK---------------------------ERAKRKFKVYKSDEDA 255
I+ P++ +Y+K E K ++ ++D+DA
Sbjct: 608 AGCTIKLPEQAIAEYLKSNITLLRWMIGEGYGDPRTLERRAQAMEAWLAKPELLEADKDA 667
Query: 256 KYYKVIEYDVTN-WEPVVAFPHLPENTVPISKAAKMNIKIDQVFIGSCTNGRIEDLRMAA 314
+Y VIE D+ + EPV+ P+ P++ +S KID+VFIGSC I R A
Sbjct: 668 EYAAVIEIDLADVKEPVLCAPNDPDDARLLSSV--QGRKIDEVFIGSCMT-NIGHFRAAG 724
Query: 315 EILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLA 374
++L+ K RL + P + + +EG I+ +AGA + P C C+G V
Sbjct: 725 KLLDKVKGGIPTRLWLAPPTKMDAHQLTEEGYYGIYGKAGARMEMPGCSLCMGNQARV-Q 783
Query: 375 SGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEE 420
+G VST+ RNF R+G ++V+LA+ +AA S++LG++ + EE
Sbjct: 784 TGSTVVSTSTRNFPNRLG-DATDVFLASAELAAVSSILGKLPTVEE 828
>pir||C82303 aconitate hydratase 2 VC0604 [imported] - Vibrio cholerae (group
O1 strain N16961) >gi|9655036|gb|AAF93771.1| (AE004145)
aconitate hydratase 2 [Vibrio cholerae]
Length = 865
Score = 119 bits (296), Expect = 5e-26
Identities = 125/463 (26%), Positives = 206/463 (43%), Gaps = 62/463 (13%)
Query: 2 GMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIA 61
G T+A+K++ + ++PG K+ V D T P+ + ++L F + +
Sbjct: 381 GYTLAQKMVGKACGVQGIRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLG-FSADLVM 439
Query: 62 IVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLIIG 121
H + + + +F G G GV H L + +LP + G
Sbjct: 440 QSFCHTSAYPKPVDVQTHHTLPDFIMNRGGVSLRPGD--GVIHSWLNRM--LLPDTVGTG 495
Query: 122 ADSHTCTYGALGAFATGV----GSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKD 177
DSHT F G+ GS +A A ATG +PE++ ++G++QP +T +D
Sbjct: 496 GDSHT-------RFPLGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRD 548
Query: 178 LILHTIGDIGVNGALY------KVMEFSGSV-----IEELSVEQRMTMSNMAIEAGAKTG 226
L+ H I G+ L K+ EFSG + +E L+VEQ +S+ + E A
Sbjct: 549 LV-HAIPYYGIKQGLLTVEKAGKINEFSGRILEIEGVEHLTVEQAFELSDASAERSAAGC 607
Query: 227 IIEPDKKTLDYV------------------KERAKRKFK----------VYKSDEDAKYY 258
++ + +++ + +R+ + + ++D+DA+Y
Sbjct: 608 TVKLSQASIEEYLNSNIVMLKWMIAEGYGDRRTIERRIQAMEAWLANPTLMEADKDAQYA 667
Query: 259 KVIEYDVTNW-EPVVAFPHLPENTVPISKAAKMNIKIDQVFIGSCTNGRIEDLRMAAEIL 317
VIE D+ EP++ P+ P++ +S+ A I ++VFIGSC I R A ++L
Sbjct: 668 HVIEIDLAEIKEPILCAPNDPDDARLLSECAGETI--NEVFIGSCMTN-IGHFRAAGKLL 724
Query: 318 EGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGE 377
E RL V P + + ++EG IF AG I P C C+G V A
Sbjct: 725 EKFNGQLNTRLWVAPPTKMDRDQLIEEGYYGIFGRAGVRIETPGCSLCMGNQARV-ADKA 783
Query: 378 RAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEE 420
+ST+ RNF R+G + VYL++ +AA A+LGRI S E
Sbjct: 784 TVMSTSTRNFPNRLG-TGANVYLSSAELAAVGAILGRIPSVAE 825
>gb|AAC35557.1| (AF086790) aconitase precursor [Homo sapiens]
Length = 600
Score = 119 bits (295), Expect = 7e-26
Identities = 86/323 (26%), Positives = 149/323 (45%), Gaps = 23/323 (7%)
Query: 115 PGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVT 174
PG L+IG DSHT G LG + GVG D MA + + + G L + +
Sbjct: 5 PGVLLIGTDSHTPNGGGLGGISIGVGGRDRVDVMAGIPCELKCTKVIAMKLTGSLSGWTS 64
Query: 175 GKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKT 234
KD+IL G + V G ++E+ G ++ +S T+ NM E GA T + + +
Sbjct: 65 PKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRM 124
Query: 235 LDYVKERA-------KRKFKVY-KSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVPISK 286
Y+ + + +FK + D +Y +VIE ++ +P + P P+ P+++
Sbjct: 125 KKYLSKTGRADIASLREEFKDHLVPDPGCQYDQVIEINLNELKPHINGPFTPDLAHPVAE 184
Query: 287 AAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILEGQKVAKWVR----LIVIPCSPT 336
+ K I IGSCTN ED+ +A + + Q +A ++ + P S
Sbjct: 185 VGTVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTITPGSEQ 243
Query: 337 VYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGH-MGVLASGER--AVSTTNRNFVGRM-G 392
+ ++G +I + G ++ CGPC+G + GE+ V++ NRNF GR
Sbjct: 244 IRATIERDGYAQILRDVGGIVLANACGPCIGQRDRKDIKKGEKNTMVTSYNRNFTGRNDA 303
Query: 393 HPKSEVYLANPYVAAASAVLGRI 415
P++ ++ +P + A A+ G +
Sbjct: 304 FPETHAFVTSPEIVTALAIAGTL 326
>sp|Q9ZL64|ACO2_HELPJ ACONITATE HYDRATASE 2 (CITRATE HYDRO-LYASE 2) (ACONITASE 2)
>gi|7463819|pir||D71896 aconitate hydratase -
Helicobacter pylori (strain J99)
>gi|4155281|gb|AAD06299.1| (AE001503) ACONITATE
HYDRATASE [Helicobacter pylori J99]
Length = 852
Score = 116 bits (288), Expect = 5e-25
Identities = 128/468 (27%), Positives = 199/468 (42%), Gaps = 66/468 (14%)
Query: 2 GMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIA 61
G T+A+KI+ + + PG K+ V D T + + +EL + FD +
Sbjct: 372 GYTLAQKIVGHACGVKGILPGAYCEPKVTTVGSQDTTGAMTRDEVKELASLK-FDAPFVL 430
Query: 62 IVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLIIG 121
H + F + G G GV H L +GL P L G
Sbjct: 431 QSFCHTAAYPKPSDVSLHATLPGFITQRGGVALHPGD--GVIHTWLNRMGL--PDTLGTG 486
Query: 122 ADSHTCTYGALGAFATGV----GSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKD 177
DSHT F G+ GS +A A TG +PE++ ++GE+ P +T +D
Sbjct: 487 GDSHT-------RFPLGISFPAGSGLVAFAAVTGTMPLNMPESVLVRFKGEMNPGITLRD 539
Query: 178 LILHTIGDIGVNGALY------KVMEFSGSVIE-----ELSVEQRMTMSNMAIEAGAKTG 226
L+ + I + L K+ F+G ++E ++ +EQ +S+ + E A
Sbjct: 540 LV-NAIPYYAIKKGLLTVEKKGKINVFNGRILEIEGLPDIKMEQAFELSDASAERSAAAC 598
Query: 227 IIEPDKKTL-DYVKERAKR---------------------------KFKVYKSDEDAKYY 258
++ +K+ + +Y+K K K + + D +A+Y
Sbjct: 599 VVRLNKEPMIEYLKSNIKLIDEMIASGYEDKETLKKRRDAMQAWVDKPVLLEPDSNAQYA 658
Query: 259 KVIEYDVTN-WEPVVAFPHLPENTVPISKA-----AKMNIKIDQVFIGSCTNGRIEDLRM 312
VIE DV EP++A P+ P++ +S+ K ID+VFIGSC I R
Sbjct: 659 AVIEIDVAEITEPILACPNDPDDVATLSEVLADTTGKRPHAIDEVFIGSCMTN-IGHFRA 717
Query: 313 AAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGV 372
EI++ ++ RL V+P S + + EG IF AGA P C C+G V
Sbjct: 718 FGEIVKNAPPSQ-ARLWVVPPSKMDEQELINEGYYAIFGAAGARTEVPGCSLCMGNQARV 776
Query: 373 LASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEE 420
ST+ RNF RMG ++VYL + + AA A+LGRI + EE
Sbjct: 777 -RDNAVVFSTSTRNFDNRMGR-GAKVYLGSAELGAACALLGRIPTKEE 822
>pir||C64617 aconitate hydratase (EC 4.2.1.3) 2 - Helicobacter pylori (strain
26695) >gi|2313908|gb|AAD07828.1| (AE000590) aconitase B
(acnB) [Helicobacter pylori 26695]
Length = 853
Score = 116 bits (287), Expect = 6e-25
Identities = 128/468 (27%), Positives = 199/468 (42%), Gaps = 66/468 (14%)
Query: 2 GMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIA 61
G T+A+KI+ + + PG K+ V D T + + +EL + FD +
Sbjct: 373 GYTLAQKIVGHACGVKGILPGTYCEPKVTTVGSQDTTGAMTRDEVKELASLK-FDAPFVL 431
Query: 62 IVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLIIG 121
H + F + G G GV H L +GL P L G
Sbjct: 432 QSFCHTAAYPKPSDVSLHATLPGFITQRGGVALHPGD--GVIHTWLNRMGL--PDTLGTG 487
Query: 122 ADSHTCTYGALGAFATGV----GSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKD 177
DSHT F G+ GS +A A TG +PE++ ++GE+ P +T +D
Sbjct: 488 GDSHT-------RFPLGISFPAGSGLVAFAAVTGTMPLNMPESVLVRFKGEMNPGITLRD 540
Query: 178 LILHTIGDIGVNGALY------KVMEFSGSVIE-----ELSVEQRMTMSNMAIEAGAKTG 226
L+ + I + L K+ F+G ++E ++ +EQ +S+ + E A
Sbjct: 541 LV-NAIPYYAIKKGLLTVEKKGKINVFNGRILEIEGLPDIKMEQAFELSDASAERSAAAC 599
Query: 227 IIEPDKKTL-DYVKERAKRKFKVYKS---------------------------DEDAKYY 258
++ +K+ + +Y+K K ++ S D +A+Y
Sbjct: 600 VVRLNKEPMIEYLKSNIKLIDEMIASGYEDKETLKKRRDAMQAWVDNPVLLEPDSNAQYA 659
Query: 259 KVIEYDVTN-WEPVVAFPHLPENTVPISKA-----AKMNIKIDQVFIGSCTNGRIEDLRM 312
VIE DV EP++A P+ P++ +S+ K ID+VFIGSC I R
Sbjct: 660 AVIEIDVAEITEPILACPNDPDDVATLSEVLADTTGKRPHAIDEVFIGSCMTN-IGHFRA 718
Query: 313 AAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGV 372
EI++ ++ RL V+P S + + EG IF AGA P C C+G V
Sbjct: 719 FGEIVKNAPPSQ-ARLWVVPPSKMDEQELINEGYYAIFGAAGARTEVPGCSLCMGNQARV 777
Query: 373 LASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEE 420
ST+ RNF RMG ++VYL + + AA A+LGRI + EE
Sbjct: 778 -RDNAVVFSTSTRNFDNRMGR-GAKVYLGSAELGAACALLGRIPTKEE 823
>sp|P56418|ACO2_HELPY ACONITATE HYDRATASE 2 (CITRATE HYDRO-LYASE 2) (ACONITASE 2)
Length = 852
Score = 116 bits (287), Expect = 6e-25
Identities = 128/468 (27%), Positives = 199/468 (42%), Gaps = 66/468 (14%)
Query: 2 GMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIA 61
G T+A+KI+ + + PG K+ V D T + + +EL + FD +
Sbjct: 372 GYTLAQKIVGHACGVKGILPGTYCEPKVTTVGSQDTTGAMTRDEVKELASLK-FDAPFVL 430
Query: 62 IVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLIIG 121
H + F + G G GV H L +GL P L G
Sbjct: 431 QSFCHTAAYPKPSDVSLHATLPGFITQRGGVALHPGD--GVIHTWLNRMGL--PDTLGTG 486
Query: 122 ADSHTCTYGALGAFATGV----GSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKD 177
DSHT F G+ GS +A A TG +PE++ ++GE+ P +T +D
Sbjct: 487 GDSHT-------RFPLGISFPAGSGLVAFAAVTGTMPLNMPESVLVRFKGEMNPGITLRD 539
Query: 178 LILHTIGDIGVNGALY------KVMEFSGSVIE-----ELSVEQRMTMSNMAIEAGAKTG 226
L+ + I + L K+ F+G ++E ++ +EQ +S+ + E A
Sbjct: 540 LV-NAIPYYAIKKGLLTVEKKGKINVFNGRILEIEGLPDIKMEQAFELSDASAERSAAAC 598
Query: 227 IIEPDKKTL-DYVKERAKRKFKVYKS---------------------------DEDAKYY 258
++ +K+ + +Y+K K ++ S D +A+Y
Sbjct: 599 VVRLNKEPMIEYLKSNIKLIDEMIASGYEDKETLKKRRDAMQAWVDNPVLLEPDSNAQYA 658
Query: 259 KVIEYDVTN-WEPVVAFPHLPENTVPISKA-----AKMNIKIDQVFIGSCTNGRIEDLRM 312
VIE DV EP++A P+ P++ +S+ K ID+VFIGSC I R
Sbjct: 659 AVIEIDVAEITEPILACPNDPDDVATLSEVLADTTGKRPHAIDEVFIGSCMTN-IGHFRA 717
Query: 313 AAEILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGV 372
EI++ ++ RL V+P S + + EG IF AGA P C C+G V
Sbjct: 718 FGEIVKNAPPSQ-ARLWVVPPSKMDEQELINEGYYAIFGAAGARTEVPGCSLCMGNQARV 776
Query: 373 LASGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEE 420
ST+ RNF RMG ++VYL + + AA A+LGRI + EE
Sbjct: 777 -RDNAVVFSTSTRNFDNRMGR-GAKVYLGSAELGAACALLGRIPTKEE 822
>pir||G81068 aconitate hydratase 2 NMB1572 [imported] - Neisseria meningitidis
(group B strain MD58) >gi|7226819|gb|AAF41925.1|
(AE002507) aconitate hydratase 2 [Neisseria meningitidis
MC58]
Length = 861
Score = 113 bits (281), Expect = 3e-24
Identities = 129/466 (27%), Positives = 209/466 (44%), Gaps = 66/466 (14%)
Query: 2 GMTIAEKILADHS---EREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
G T+A+K++ E + V+PG ++ V D T P+ + ++L F +
Sbjct: 379 GFTLAQKMVGRACGLPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLG-FSAD 437
Query: 59 RIAIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDL 118
+ H + K F G G GV H L L +LP +
Sbjct: 438 MVMQSFCHTAAYPKPVDVKTHKELPAFISTRGGVSLRPGD--GVIHSWLNRL--LLPDTV 493
Query: 119 IIGADSHTCTYGALGAFATGV----GSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVT 174
G DSHT F G+ GS +A A ATG +PE++ + G+LQP VT
Sbjct: 494 GTGGDSHT-------RFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVT 546
Query: 175 GKDLILHTIGDIGVNGALYKVME------FSGSVIE-----ELSVEQRMTMSNMAIEAGA 223
+DL+ + I + L V + FSG ++E +L VEQ +++ + E A
Sbjct: 547 LRDLV-NAIPLYAIKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSA 605
Query: 224 KTGIIEPDKK-TLDYVKERA-----------------KRKFK----------VYKSDEDA 255
++ +K+ ++Y+K +R+ K + ++D+DA
Sbjct: 606 AGCTVKLNKEPIIEYMKSNVVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDA 665
Query: 256 KYYKVIEYDVTN-WEPVVAFPHLPENTVPISKAAKMNIKIDQVFIGSCTNGRIEDLRMAA 314
+Y VIE ++ + EP++A P+ P++ +S+ + KID+VFIGSC I R A+
Sbjct: 666 EYAAVIEINMDDIKEPIIACPNDPDDVCFMSE--RSGTKIDEVFIGSCMT-NIGHFRAAS 722
Query: 315 EILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLA 374
++LEG K VRL + P + + EG + AGA + P C C+G V
Sbjct: 723 KLLEG-KADTPVRLWIAPPTKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQV-R 780
Query: 375 SGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEE 420
G +ST+ RNF R+G + VYL + +AA + LG+I + EE
Sbjct: 781 EGATVMSTSTRNFPNRLG-KNTFVYLGSAELAAICSKLGKIPTVEE 825
>pir||A81801 aconitate hydratase (EC 4.2.1.3) NMA1761 [imported] - Neisseria
meningitidis (group A strain Z2491)
>gi|7380403|emb|CAB84989.1| (AL162757) aconitate
hydratase [Neisseria meningitidis Z2491]
Length = 861
Score = 113 bits (281), Expect = 3e-24
Identities = 129/466 (27%), Positives = 209/466 (44%), Gaps = 66/466 (14%)
Query: 2 GMTIAEKILADHS---EREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
G T+A+K++ E + V+PG ++ V D T P+ + ++L F +
Sbjct: 379 GFTLAQKMVGRACGLPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLG-FSAD 437
Query: 59 RIAIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDL 118
+ H + K F G G GV H L L +LP +
Sbjct: 438 MVMQSFCHTAAYPKPVDVKTHKELPAFISTRGGVSLRPGD--GVIHSWLNRL--LLPDTV 493
Query: 119 IIGADSHTCTYGALGAFATGV----GSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVT 174
G DSHT F G+ GS +A A ATG +PE++ + G+LQP VT
Sbjct: 494 GTGGDSHT-------RFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVT 546
Query: 175 GKDLILHTIGDIGVNGALYKVME------FSGSVIE-----ELSVEQRMTMSNMAIEAGA 223
+DL+ + I + L V + FSG ++E +L VEQ +++ + E A
Sbjct: 547 LRDLV-NAIPLYAIKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSA 605
Query: 224 KTGIIEPDKK-TLDYVKERA-----------------KRKFK----------VYKSDEDA 255
++ +K+ ++Y+K +R+ K + ++D+DA
Sbjct: 606 AGCTVKLNKEPIIEYMKSNVVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDA 665
Query: 256 KYYKVIEYDVTN-WEPVVAFPHLPENTVPISKAAKMNIKIDQVFIGSCTNGRIEDLRMAA 314
+Y VIE ++ + EP++A P+ P++ +S+ + KID+VFIGSC I R A+
Sbjct: 666 EYAAVIEINMDDIKEPIIACPNDPDDVCFMSE--RSGTKIDEVFIGSCMT-NIGHFRAAS 722
Query: 315 EILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLA 374
++LEG K VRL + P + + EG + AGA + P C C+G V
Sbjct: 723 KLLEG-KADTPVRLWIAPPTKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQV-R 780
Query: 375 SGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEE 420
G +ST+ RNF R+G + VYL + +AA + LG+I + EE
Sbjct: 781 EGATVMSTSTRNFPNRLG-KNTFVYLGSAELAAICSKLGKIPTVEE 825
>pir||A82825 aconitate hydratase 2 XF0292 [imported] - Xylella fastidiosa
(strain 9a5c) >gi|9105113|gb|AAF83105.1|AE003882_7
(AE003882) aconitate hydratase 2 [Xylella fastidiosa]
Length = 886
Score = 113 bits (281), Expect = 3e-24
Identities = 131/466 (28%), Positives = 205/466 (43%), Gaps = 62/466 (13%)
Query: 2 GMTIAEKILAD-------HSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRV 54
G ++A+KI+ + +++ ++PG K+ V D T P+ + ++L
Sbjct: 400 GFSLAQKIVGRACGLPEVNGKQQGMRPGTYCEPKMTSVGSQDTTGPMTRDELKDLACLG- 458
Query: 55 FDRERIAIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVL 114
F + + H + + EF G G GV H L + +L
Sbjct: 459 FSADLVMQSFCHTAAYPKPVDVKTHHTLPEFISTRGGVSLRPGD--GVIHSWLNRM--LL 514
Query: 115 PGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVT 174
P + G DSHT L +F G G +A A ATG +PE++ ++G +QP VT
Sbjct: 515 PDTVGTGGDSHTRFPIGL-SFPAGSGL--VAFAAATGVMPLDMPESVLVRFKGHMQPGVT 571
Query: 175 GKDLILHTIGDIGVNGALYKVME------FSGSVIE-----ELSVEQRMTMSNMAIEAGA 223
+DL+ + I + L V + FSG ++E L VEQ +S+ + E A
Sbjct: 572 LRDLV-NAIPLYAIKQGLLTVAKQGKKNIFSGRILEIEGLPNLKVEQAFELSDASAERSA 630
Query: 224 --------KTGIIEPDKKTLDYVK-------------ERAKRKFKVY-------KSDEDA 255
K IIE + +K R +K + + + D D
Sbjct: 631 AGCTVRLNKEPIIEYLNSNITLLKWMIAEGYADARSLTRRIKKMEAWLADPQLIEPDTDV 690
Query: 256 KYYKVIEYDVTN-WEPVVAFPHLPENTVPISKAAKMNIKIDQVFIGSCTNGRIEDLRMAA 314
+Y VIE D+ + EP+VA P+ P++ +S+ A KID+VFIGSC I R AA
Sbjct: 691 EYAAVIEIDLADIHEPIVACPNDPDDVKTLSEVA--GAKIDEVFIGSCMT-NIGHFRAAA 747
Query: 315 EILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLA 374
++LEG++ RL V P + + KEG F AGA + P C C+ G+
Sbjct: 748 KLLEGKRDIP-TRLWVAPPTKMDASELTKEGYYGTFGNAGARMEMPGCSLCM-GNQAQAR 805
Query: 375 SGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEE 420
G ST+ RNF R+G + VYL + +AA + LG+I + +E
Sbjct: 806 EGATVFSTSTRNFPNRLGR-NTNVYLGSAELAAICSRLGKIPTKDE 850
>gb|AAD32176.1|AF125563_1 (AF125563) putative aconitate hydratase [Neisseria meningitidis]
Length = 861
Score = 113 bits (279), Expect = 5e-24
Identities = 129/466 (27%), Positives = 209/466 (44%), Gaps = 66/466 (14%)
Query: 2 GMTIAEKILADHS---EREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRE 58
G T+A+K++ E + V+PG ++ V D T P+ + ++L F +
Sbjct: 379 GFTLAQKMVGRACGLPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLG-FSAD 437
Query: 59 RIAIVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDL 118
+ H + K F G G GV H L L +LP +
Sbjct: 438 MVMQSFCHTAAYPKPVDVKTHKELPAFISTRGGVSLRPGD--GVIHSWLNRL--LLPDTV 493
Query: 119 IIGADSHTCTYGALGAFATGV----GSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVT 174
G DSHT F G+ GS +A A ATG +PE++ + G+LQP VT
Sbjct: 494 GTGGDSHT-------RFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVT 546
Query: 175 GKDLILHTIGDIGVNGALYKVME------FSGSVIE-----ELSVEQRMTMSNMAIEAGA 223
+DL+ + I + L V + FSG ++E +L VEQ +++ + E A
Sbjct: 547 LRDLV-NAIPLYAIKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSA 605
Query: 224 KTGIIEPDKK-TLDYVKERA-----------------KRKFK----------VYKSDEDA 255
++ +K+ ++Y+K +R+ K + ++D+DA
Sbjct: 606 AGCTVKLNKEPIIEYMKSNVVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDA 665
Query: 256 KYYKVIEYDVTN-WEPVVAFPHLPENTVPISKAAKMNIKIDQVFIGSCTNGRIEDLRMAA 314
+Y VIE ++ + EP++A P+ P++ +S+ + KID+VFIGSC I R A+
Sbjct: 666 EYAAVIEINMDDIKEPIIACPNDPDDVCFMSE--RSGTKIDEVFIGSCMT-NIGHFRAAS 722
Query: 315 EILEGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLA 374
++LEG+ VRL V P + + EG + AGA + P C C+G V
Sbjct: 723 KLLEGKSDIP-VRLWVAPPTKMDAKELSDEGHYGVLGRAGARMEMPGCSLCMGNQAQV-H 780
Query: 375 SGERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEE 420
G +ST+ RNF R+G + VYL + +AA + LG+I + EE
Sbjct: 781 EGATVMSTSTRNFPNRLG-KNTFVYLGSAELAAICSKLGKIPTVEE 825
>emb|CAA89720.1| (Z49701) unknown [Saccharomyces cerevisiae]
Length = 375
Score = 112 bits (277), Expect = 9e-24
Identities = 62/146 (42%), Positives = 87/146 (59%), Gaps = 7/146 (4%)
Query: 284 ISKAAKMNIKIDQVFIGSCTNGRIEDLRMAAEIL-------EGQKVAKWVRLIVIPCSPT 336
+ ++ +IKI++ ++ SCTN R+ DL+ AA+++ + KVA V V S
Sbjct: 4 VQDLSQQDIKINKAYLVSCTNSRLSDLQSAADVVCPTGDLNKVNKVAPGVEFYVAAASSE 63
Query: 337 VYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGERAVSTTNRNFVGRMGHPKS 396
+ A K G E L+AG + P CGPC+G G+L GE +S TNRNF GRMG +
Sbjct: 64 IEADARKSGAWEKLLKAGCIPLPSGCGPCIGLGAGLLEPGEVGISATNRNFKGRMGSKDA 123
Query: 397 EVYLANPYVAAASAVLGRIASPEEVV 422
YLA+P V AASAVLG+I+SP EV+
Sbjct: 124 LAYLASPAVVAASAVLGKISSPAEVL 149
>sp|P74582|ACO2_SYNY3 ACONITATE HYDRATASE 2 (CITRATE HYDRO-LYASE 2) (ACONITASE 2)
>gi|7469257|pir||S76777 aconitate hydratase (EC 4.2.1.3)
2 - Synechocystis sp. (strain PCC 6803)
>gi|1653778|dbj|BAA18689.1| (D90916) hypothetical
protein [Synechocystis sp.]
Length = 868
Score = 111 bits (276), Expect = 1e-23
Identities = 131/463 (28%), Positives = 201/463 (43%), Gaps = 63/463 (13%)
Query: 2 GMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERIA 61
G T+A+K++ + ++PG + V D T P+ + +EL F+ +
Sbjct: 371 GFTLAQKMVGKACGVQGIRPGTSCEPIMTTVGSQDTTGPMTRDELKELACLG-FNADLTL 429
Query: 62 IVLDHFTPNKDIKSAEQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPELGLVLPGDLIIG 121
H + K +F G G G+ H L + +LP + G
Sbjct: 430 QTFCHTAAYPKPVDIKTHKDLPDFFSTRGGVALRPGD--GIIHSWLNRM--LLPDTVGTG 485
Query: 122 ADSHTCTYGALGAFATGV----GSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKD 177
DSHT F G+ GS +A A A G +PE++ + GELQP VT +D
Sbjct: 486 GDSHT-------RFPLGISFPAGSGLVAFAAALGVMPLDMPESVLVKFTGELQPGVTLRD 538
Query: 178 LILHTIGDIGVN------GALYKVMEFSGSVIE-----ELSVEQRMTMSNMAIEAGAKTG 226
I++ I + + G KV F+G ++E +L VEQ +++ E
Sbjct: 539 -IVNAIPWVAMQQGKLTVGKGDKVNVFNGRIMEMEGLPDLKVEQAFELTDATAERSCSGS 597
Query: 227 IIEPDKKTL-DY-------VKERAKRKFK--------------------VYKSDEDAKYY 258
I+ ++T+ +Y +K R ++ + D DA+Y
Sbjct: 598 TIKLSEETVAEYLRSNVVLMKNMIARGYQDARTLLRRIAKMEEWLANPSLMSGDADAEYA 657
Query: 259 KVIEYDVTN-WEPVVAFPHLPENTVPISKAAKMNIKIDQVFIGSCTNGRIEDLRMAAEIL 317
VIE ++ EP+VA P+ P+N +S+ A I +VFIGSC I R AA+IL
Sbjct: 658 DVIEVNLDEIKEPIVAAPNDPDNVKLMSECA--GDVIHEVFIGSCMT-NIGHYRAAAKIL 714
Query: 318 EGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGE 377
EG K RL + P + + +EG+ IF AGA P C C+G V G
Sbjct: 715 EGAGTVKG-RLWICPPTRMDEQQLREEGIYGIFAAAGARTEMPGCSLCMGNQARV-EDGV 772
Query: 378 RAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRIASPEE 420
ST+ RNF RMG ++VYL + +AA A+LG+I + EE
Sbjct: 773 TVFSTSTRNFNNRMG-KGAQVYLGSAELAAVCALLGKIPTVEE 814
>gb|AAK41354.1| Aconitate hydratase [Sulfolobus solfataricus]
Length = 855
Score = 108 bits (267), Expect = 1e-22
Identities = 97/339 (28%), Positives = 146/339 (42%), Gaps = 41/339 (12%)
Query: 117 DLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWF-RVPETMKFIYEGELQPYVTG 175
++IIG DSHT LG GVG + A A+ GE ++ VPE + GE+Q VT
Sbjct: 194 EVIIGTDSHTTMIEGLGILGWGVGGLE-AEAVLLGEPYYLNVPEVIGVRLTGEIQEGVTP 252
Query: 176 KDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTL 235
D++L+ + + K +EF G + LSV R T++NMA E GA D T+
Sbjct: 253 TDVVLYITELLRKKNVVSKFVEFFGPSLSLLSVPDRATIANMAPEYGATAAYFPIDDVTV 312
Query: 236 DY----------VKERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTV--- 282
Y VK+ A+ + Y +Y ++E D++ EP +A P P+ +
Sbjct: 313 SYLELTNRDGEFVKKYAQLQELFYDDSRKIRYSDIVEVDLSKIEPAIAGPRNPDERISLR 372
Query: 283 ----PISKAAKMNIK------IDQVFIGSCTNGRIEDLRMAAEILE------GQKVAKWV 326
+SK K K + I SCTN + + A IL G +V ++
Sbjct: 373 EVKGKLSKEKKKKGKYVEDNAVVLAAITSCTNTSNPTVMLGAGILAKKAVEMGLRVPPYI 432
Query: 327 RLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGG--------HMGVLASG-- 376
+ P SP V + GL+ G + C C+G + +G
Sbjct: 433 KTSTAPGSPVVAEYLKETGLLPYLEALGFHLVGFGCTTCIGNAGPLPKYVEEDIKENGIE 492
Query: 377 ERAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRI 415
AV + NRNF GR+ +LA+P + A A+ GRI
Sbjct: 493 TYAVISGNRNFEGRINPLLKGTFLASPILVVAYALAGRI 531
>gb|AAF09127.1|AF088222_2 (AF088222) aconitate hydratase [Lactococcus lactis subsp. lactis]
Length = 848
Score = 103 bits (255), Expect = 3e-21
Identities = 98/349 (28%), Positives = 145/349 (41%), Gaps = 46/349 (13%)
Query: 111 GLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQ 170
GL+ P D + G DSHT ALG GVG + M + VPE + G+L
Sbjct: 183 GLLYP-DSVFGTDSHTTMINALGVLGWGVGGIEAEAVMLGEPSSLTVPEVVGVRLIGKLG 241
Query: 171 PYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEP 230
+ T DL L + + K +EF G ++ LS+ R T++NMA E GA G
Sbjct: 242 VFATATDLALTVTKILRDEKVVGKFVEFFGEGLKNLSLADRATIANMAPEYGATCGYFPI 301
Query: 231 DKKTLDY-------------VKERAKRKFKVYKSDEDAKYYKVIEYDVTNWEPVVAFPHL 277
D +TL Y V+ AK Y Y KVI+ ++ E ++ P
Sbjct: 302 DNETLKYLRATNRSEEIVARVEAYAKANKLFYDDSYQPHYSKVIDINLEKVETRLSGPSR 361
Query: 278 PENTVPI--------------SKAAKMNIKIDQVFIGSCTNGRIEDLRMAAEIL------ 317
P++ + + K + + KI I SCTN + M A +L
Sbjct: 362 PQDMLELGDLSHNFEKFVEQQKKLKEEDGKIAIAAITSCTNTSNPHVMMMAGLLAKNAVE 421
Query: 318 EGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGVLASGE 377
+G +V WV+ + P S V K GL+ G + C C+G +L E
Sbjct: 422 QGLEVPSWVKTSLAPGSKVVSSYLDKAGLLPYLETLGFNVIGYGCTTCIGNSGPLLREME 481
Query: 378 R----------AVSTTNRNFVGRMGHPKSEV-YLANPYVAAASAVLGRI 415
+ +V + NRNF GR+ HP + YLA+P + A A+ G +
Sbjct: 482 KEITDKELVATSVLSGNRNFEGRI-HPLVKANYLASPPLVVAYALAGSV 529
>sp|P37032|ACON_LEGPN ACONITATE HYDRATASE (CITRATE HYDRO-LYASE) (ACONITASE) (MAJOR
IRON-CONTAINING PROTEIN) (MICP) (IP210)
>gi|538712|pir||B48642 aconitate hydratase (EC 4.2.1.3)
- Legionella pneumophila >gi|348945|gb|AAA25295.1|
(L22081) aconitase [Legionella pneumophila]
Length = 891
Score = 99.1 bits (243), Expect = 9e-20
Identities = 94/360 (26%), Positives = 146/360 (40%), Gaps = 60/360 (16%)
Query: 117 DLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGK 176
D ++G DSHT LG GVG + AM +PE + F G+L+ +T
Sbjct: 208 DTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVIGFKLSGKLKEGITAT 267
Query: 177 DLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLD 236
DL+L + G + K +EF G + +L + R T+SNMA E GA G DK+T+
Sbjct: 268 DLVLTVTQMLRKKGVVGKFVEFYGPGLNDLPLADRATISNMAPEYGATCGFFPVDKETIK 327
Query: 237 YVKERAKRKFKVY--------------KSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTV 282
Y++ + K + K +E+ + + D+ + EP +A P P++ V
Sbjct: 328 YLELTGRDKHTIALVEAYAKAQGMWYDKDNEEPVFTDSLHLDLGSVEPSLAGPKRPQDKV 387
Query: 283 PIS-------------------------KAAKMNIKIDQVFIG---SCTNGRIEDLRMAA 314
+S K +K V I SCTN + MAA
Sbjct: 388 NLSSLPVEFNNFLIEVGKEKEKEKTFAVKNKDFQMKHGHVVIAAITSCTNTSNPSVLMAA 447
Query: 315 EILEGQKVAK------WVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGG 368
++ + + K WV+ + P S V GL + G + C C+G
Sbjct: 448 GLVAKKAIEKGLQRKPWVKSSLAPGSKVVTDYLRHAGLQTYLDQLGFNLVGYGCTTCIGN 507
Query: 369 H-------MGVLASGERAVSTT---NRNFVGRMGHPKSEV-YLANPYVAAASAVLGRIAS 417
+A + VS+ NRNF GR+ HP+ +LA+P + A A+ G S
Sbjct: 508 SGPLPDDISHCVAEHDLVVSSVLSGNRNFEGRV-HPQVRANWLASPPLVVAYALCGTTCS 566
>sp|P25516|ACO1_ECOLI ACONITATE HYDRATASE 1 (CITRATE HYDRO-LYASE 1) (ACONITASE 1)
>gi|281806|pir||S22375 aconitate hydratase (EC 4.2.1.3)
- Escherichia coli >gi|40896|emb|CAA42834.1| (X60293)
aconitate hydratase [Escherichia coli]
>gi|1742087|dbj|BAA14828.1| (D90765) Aconitate hydratase
(EC 4.2.1.3) [Escherichia coli]
Length = 891
Score = 96.3 bits (236), Expect = 6e-19
Identities = 95/362 (26%), Positives = 148/362 (40%), Gaps = 66/362 (18%)
Query: 117 DLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGK 176
D ++G DSHT LG GVG + AM +P+ + F G+L+ +T
Sbjct: 206 DTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITAT 265
Query: 177 DLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLD 236
DL+L + +G + K +EF G ++ L + R T++NM+ E GA G D TLD
Sbjct: 266 DLVLTVTQMLRKHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLD 325
Query: 237 YVKERAKRKFKVYKSDEDAK-------------YYKVIEYDVTNWEPVVAFPHLPENTV- 282
Y++ + + +V ++ AK + +E D+ + E +A P P++ V
Sbjct: 326 YMRLSGRSEDQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVA 385
Query: 283 ----PISKAAKMNIKIDQVF--------------------------IGSCTNGRIEDLRM 312
P + AA ++++ I SCTN + M
Sbjct: 386 LPDVPKAFAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLM 445
Query: 313 AAEILE------GQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCL 366
AA +L G K WV+ + P S V K L E G + C C+
Sbjct: 446 AAGLLAKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCI 505
Query: 367 GGH------------MGVLASGERAVSTTNRNFVGRMGHPKSEV-YLANPYVAAASAVLG 413
G G L G AV + NRNF GR+ HP + +LA+P + A A+ G
Sbjct: 506 GNSGPLPDPIETAIKKGDLTVG--AVLSGNRNFEGRI-HPLVKTNWLASPPLVVAYALAG 562
Query: 414 RI 415
+
Sbjct: 563 NM 564
>pir||G64875 aconitate hydratase (EC 4.2.1.3) - Escherichia coli
>gi|1787531|gb|AAC74358.1| (AE000225) aconitate hydrase
1 [Escherichia coli K12]
Length = 891
Score = 95.2 bits (233), Expect = 1e-18
Identities = 92/360 (25%), Positives = 147/360 (40%), Gaps = 62/360 (17%)
Query: 117 DLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGK 176
D ++G DSHT LG GVG + AM +P+ + F G+L+ +T
Sbjct: 206 DTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITAT 265
Query: 177 DLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLD 236
DL+L + +G + K +EF G ++ L + R T++NM+ E GA G D TLD
Sbjct: 266 DLVLTVTQMLRKHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLD 325
Query: 237 YVKERAKRKFKVYKSDEDAK-------------YYKVIEYDVTNWEPVVAFPHLPENTV- 282
Y++ + + +V ++ AK + +E D+ + E +A P P++ V
Sbjct: 326 YMRLSGRSEDQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVA 385
Query: 283 ----PISKAAKMNIKIDQVF--------------------------IGSCTNGRIEDLRM 312
P + AA ++++ I SCTN + M
Sbjct: 386 LPDVPKAFAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLM 445
Query: 313 AAEILE------GQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCL 366
AA +L G K WV+ + P S V K L E G + C C+
Sbjct: 446 AAGLLAKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCI 505
Query: 367 GGHMGVLASGERAVSTT----------NRNFVGRMGHPKSEV-YLANPYVAAASAVLGRI 415
G + E A+ + NRNF GR+ HP + +LA+P + A A+ G +
Sbjct: 506 GNSGPLPDPIETAIKKSDLTVGAVLSGNRNFEGRI-HPLVKTNWLASPPLVVAYALAGNM 564
>pir||A83547 probable aconitate hydratase PA0794 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9946685|gb|AAG04183.1|AE004514_6 (AE004514) probable
aconitate hydratase [Pseudomonas aeruginosa]
Length = 868
Score = 94.8 bits (232), Expect = 2e-18
Identities = 92/353 (26%), Positives = 144/353 (40%), Gaps = 49/353 (13%)
Query: 111 GLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQ 170
G+ P D +G DSHT ALG A GVG + M +W R+P+ + G Q
Sbjct: 189 GVAFP-DTCVGTDSHTPHVDALGVIAIGVGGLEAENVMLGRASWMRLPDIVGVELSGRRQ 247
Query: 171 PYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEP 230
P +T D++L + + +EF G L++ R T+SNMA E GA +
Sbjct: 248 PGITATDVVLALTEFLRKQKVVGAYLEFYGEGASSLTLGDRATISNMAPEYGATAAMFAI 307
Query: 231 DKKTLDYVKERAKRKFKV-------------YKSDEDAKYYKVIEYDVTNWEPVVAFPHL 277
D++T+DY++ + +V S DA+Y +V+++D+++ +A P
Sbjct: 308 DQQTIDYLRLTGRDDEQVALVEAYARTAGLWADSLVDAEYERVLKFDLSSVVRNMAGPSN 367
Query: 278 PENTVPISKAAKMNI-------------------KIDQVFIGSCTNGRIEDLRMAAEILE 318
P V S+ A I + I SCTN +AA +L
Sbjct: 368 PHARVATSELAAKGIAGNLERARAEEAEGLMPDGAVIIAAITSCTNTSNPRNVIAAGLLA 427
Query: 319 ------GQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGGHMGV 372
G WV+ + P S V + GL+ G + C C G +
Sbjct: 428 RNADRLGLVRKPWVKTSLAPGSKVVTEYLREAGLLPHLEALGFGVVAYACTSCNGMSGAL 487
Query: 373 LASGER----------AVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRI 415
+ +R AV + NRNF GR+ + +LA+P + A A+ G I
Sbjct: 488 DPAIQREIVERDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTI 540
>sp|Q9ZCF4|ACON_RICPR ACONITATE HYDRATASE (CITRATE HYDRO-LYASE) (ACONITASE)
>gi|7437037|pir||A71641 aconitate hydratase (acnA) RP799
- Rickettsia prowazekii >gi|3861326|emb|CAA15225.1|
(AJ235273) ACONITATE HYDRATASE (acnA) [Rickettsia
prowazekii]
Length = 878
Score = 89.7 bits (219), Expect = 6e-17
Identities = 85/357 (23%), Positives = 139/357 (38%), Gaps = 58/357 (16%)
Query: 117 DLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGK 176
D ++G DSHT L GVG + AM +PE + G+L T
Sbjct: 199 DSLVGTDSHTTMVNGLSVLGWGVGGIEAESAMLGQPITMIIPEVIGVKLIGKLAGMATAT 258
Query: 177 DLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLD 236
DL+L + + K +EF G+ + L++ R T+SNM+ E GA G D++TL
Sbjct: 259 DLVLTITEILRRKKVVGKFVEFFGNGLRNLTISDRATISNMSPEYGATCGFFPIDQETLK 318
Query: 237 YVKERAKRKFKV-------------YKSDEDAKYYKVIEYDVTNWEPVVAFPHLPENTVP 283
Y++ + ++ Y ++ +Y +V+E D++ +A P P++ V
Sbjct: 319 YLEVTGREITQIKLVEKYAIEQNLWYNCEDTQEYTEVLELDLSTVYSSLAGPKRPQDRVN 378
Query: 284 ISKAAK-----------MNIKIDQVF------------------IGSCTNGRIEDLRMAA 314
++ A N ID+ + I SCTN + + A
Sbjct: 379 LNCVASNFQNELPYFALANKDIDKKYAVANQNYAIGNGDVVIAAITSCTNTSNPSVMIGA 438
Query: 315 EIL------EGQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLGG 368
+L G V WV+ + P S V GL + G + C C+G
Sbjct: 439 ALLAKKALEHGLNVKPWVKTSLAPGSKVVTEYLKLSGLDKYLDALGFNLVGYGCTTCIGN 498
Query: 369 HMGVLASGERAVS----------TTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRI 415
+ E ++ + NRNF GR+ YLA+P + A A+ G +
Sbjct: 499 SGSLNPEIENTINKNRLVVASVLSGNRNFEGRINPLTKASYLASPILVVAYALSGTL 555
>dbj|BAA21004.1| (D17631) ORF [Escherichia coli] >gi|4433055|dbj|BAA21005.1|
(D17632) 2-isopropylmalate dehydratase alpha subunit
[Escherichia coli]
Length = 205
Score = 85.0 bits (207), Expect = 1e-15
Identities = 66/211 (31%), Positives = 97/211 (45%), Gaps = 17/211 (8%)
Query: 1 MGMTIAEKILADHSEREEVKPGEIVMAKLDFVFGNDVTMPLAIKKFRELGVKRVFDRERI 60
M T+ EK+ H E E + +D ++VT P A R G + V +
Sbjct: 1 MAKTLYEKLFDAHVVYEA--ENETPLLYIDRHLVHEVTSPQAFDGLRAHG-RPVRQPGKT 57
Query: 61 AIVLDH--FTPNKDIKSA---------EQCKSSREFAKEMGIKWFFEGGSVGVEHCLLPE 109
+DH T KDI E K+ +EF E+ + G+ H + PE
Sbjct: 58 FATMDHNVSTQTKDINGCGEMARIQMQELIKNCKEFGVEL---YDLNHPYQGIVHVMGPE 114
Query: 110 LGLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGEL 169
G+ LPG I+ DSHT T+GA GA A G+G++++ +AT +TMK +G+
Sbjct: 115 QGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVQGKA 174
Query: 170 QPYVTGKDLILHTIGDIGVNGALYKVMEFSG 200
P +T KD++L IG G G V+EF G
Sbjct: 175 APGITAKDIVLAIIGKTGSAGGTGHVVEFCG 205
>pir||C81200 aconitate hydratase 1 NMB0433 [imported] - Neisseria meningitidis
(group B strain MD58) >gi|7225657|gb|AAF40871.1|
(AE002399) aconitate hydratase 1 [Neisseria meningitidis
MC58]
Length = 868
Score = 83.5 bits (203), Expect = 4e-15
Identities = 87/349 (24%), Positives = 138/349 (38%), Gaps = 45/349 (12%)
Query: 111 GLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQ 170
G+ P D +G DSHT +LG + GVG + M + R+P+ + G+ Q
Sbjct: 194 GVAFP-DTCVGTDSHTPHVDSLGVISVGVGGLEAETVMLGRASMMRLPDIVGVELNGKRQ 252
Query: 171 PYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEP 230
+T D++L + + +EF G LS+ R T+SNM E GA +
Sbjct: 253 AGITATDIVLALTEFLRKERVVGAFVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAI 312
Query: 231 DKKTLDYVKERAKRKFKVYKSDEDAK-------------YYKVIEYDVTNWEPVVAFPHL 277
D++T+DY+K + +V + AK Y +V+++D+++ +A P
Sbjct: 313 DEQTIDYLKLTGRDDAQVKLVETYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSN 372
Query: 278 PENTVPISKAAKMNI---------------KIDQVFIGSCTNGRIEDLRMAAEILE---- 318
P + A + + I SCTN +AA +L
Sbjct: 373 PHARFATADLAAKGLAKPYEEPSDGQMPDGSVIIAAITSCTNTSNPRNVVAAALLARNAN 432
Query: 319 --GQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG--GHMGVLA 374
G K WV+ P S + GL+ + G I C C G G +
Sbjct: 433 RLGLKRKPWVKSSFAPGSKVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKI 492
Query: 375 SGE--------RAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRI 415
E AV + NRNF GR+ + +LA+P + A A+ G I
Sbjct: 493 QKEIIDRDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYALAGSI 541
>pir||H81775 aconitate hydratase (EC 4.2.1.3) NMA2052 [imported] - Neisseria
meningitidis (group A strain Z2491)
>gi|7380680|emb|CAB85270.1| (AL162758) aconitate
hydratase [Neisseria meningitidis Z2491]
Length = 868
Score = 78.0 bits (189), Expect = 2e-13
Identities = 85/349 (24%), Positives = 137/349 (38%), Gaps = 45/349 (12%)
Query: 111 GLVLPGDLIIGADSHTCTYGALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQ 170
G+ P D +G DSHT ALG + GVG + M + R+P+ + G+ +
Sbjct: 194 GVAFP-DTCVGTDSHTPHVDALGVISVGVGGLEAETVMLGRASMMRLPDIVGVELNGKRK 252
Query: 171 PYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEP 230
+T D++L + + +EF G LS+ R T+SNM E GA +
Sbjct: 253 AGITATDIVLALTEFLRKERVVGAFVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAI 312
Query: 231 DKKTLDYVKERAKRKFKVYKSDEDAK-------------YYKVIEYDVTNWEPVVAFPHL 277
D++T+DY+K + +V + AK Y +V+++D+++ +A P
Sbjct: 313 DEQTIDYLKLTGRDDAQVKLVETYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSN 372
Query: 278 PENTVPISKAAKMNI---------------KIDQVFIGSCTNGRIEDLRMAAEILE---- 318
P + A + + I SCTN +AA +L
Sbjct: 373 PHARFATADLAGKGLAKPYEEPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNAN 432
Query: 319 --GQKVAKWVRLIVIPCSPTVYWKALKEGLIEIFLEAGAVIGPPTCGPCLG--GHMGVLA 374
G + WV+ P S + L+ + G I C C G G +
Sbjct: 433 RLGLQRKPWVKSSFAPGSKVAEIYLKEADLLPEMEKLGFGIVAFACTTCNGMSGALDPKI 492
Query: 375 SGE--------RAVSTTNRNFVGRMGHPKSEVYLANPYVAAASAVLGRI 415
E AV + NRNF GR+ + +LA+P + A A+ G I
Sbjct: 493 QKEIIDRDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYALAGSI 541
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.319 0.137 0.404
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 157160335
Number of Sequences: 2977
Number of extensions: 6717903
Number of successful extensions: 16140
Number of sequences better than 1.0e-10: 187
Number of HSP's better than 0.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 52
Number of HSP's that attempted gapping in prelim test: 15552
Number of HSP's gapped (non-prelim): 268
length of query: 423
length of database: 189,106,746
effective HSP length: 56
effective length of query: 367
effective length of database: 155,591,474
effective search space: 57102070958
effective search space used: 57102070958
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 166 (69.1 bits)